LOCUS       DAZKOR010000001        76162 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000001 DAZKOR010000000
VERSION     DAZKOR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.001"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..1491)
                     /gene="tyrP"
                     /locus_tag="ACG8ZM_000001"
     CDS             complement(70..1491)
                     /gene="tyrP"
                     /locus_tag="ACG8ZM_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0491003.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="ACG8ZM_000002"
     CDS             complement(1734..3611)
                     /gene="tdc"
                     /locus_tag="ACG8ZM_000002"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0491004.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3902..5158)
                     /gene="tyrS"
                     /locus_tag="ACG8ZM_000003"
     CDS             complement(3902..5158)
                     /gene="tyrS"
                     /locus_tag="ACG8ZM_000003"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0491005.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5270..5475)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5738..7039
                     /locus_tag="ACG8ZM_000004"
     CDS             5738..7039
                     /locus_tag="ACG8ZM_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0491006.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7108..7707)
                     /locus_tag="ACG8ZM_000005"
     CDS             complement(7108..7707)
                     /locus_tag="ACG8ZM_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0491007.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            7962..8843
                     /locus_tag="ACG8ZM_000006"
     CDS             7962..8843
                     /locus_tag="ACG8ZM_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0491008.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            8856..10466
                     /locus_tag="ACG8ZM_000007"
     CDS             8856..10466
                     /locus_tag="ACG8ZM_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0491009.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(10543..11202)
                     /locus_tag="ACG8ZM_000008"
     CDS             complement(10543..11202)
                     /locus_tag="ACG8ZM_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0491010.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(11485..12150)
                     /locus_tag="ACG8ZM_000009"
     CDS             complement(11485..12150)
                     /locus_tag="ACG8ZM_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0491011.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(12297..12902)
                     /gene="dhaL"
                     /locus_tag="ACG8ZM_000010"
     CDS             complement(12297..12902)
                     /gene="dhaL"
                     /locus_tag="ACG8ZM_000010"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0491012.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(12930..13940)
                     /gene="dhaK"
                     /locus_tag="ACG8ZM_000011"
     CDS             complement(12930..13940)
                     /gene="dhaK"
                     /locus_tag="ACG8ZM_000011"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0491013.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(13915..14310)
                     /gene="dhaM"
                     /locus_tag="ACG8ZM_000012"
     CDS             complement(13915..14310)
                     /gene="dhaM"
                     /locus_tag="ACG8ZM_000012"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0491014.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            14464..14784
                     /locus_tag="ACG8ZM_000013"
     CDS             14464..14784
                     /locus_tag="ACG8ZM_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0491015.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(14896..15753)
                     /locus_tag="ACG8ZM_000014"
     CDS             complement(14896..15753)
                     /locus_tag="ACG8ZM_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0491016.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(15861..17165)
                     /locus_tag="ACG8ZM_000015"
     CDS             complement(15861..17165)
                     /locus_tag="ACG8ZM_000015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0491017.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(17162..17671)
                     /locus_tag="ACG8ZM_000016"
     CDS             complement(17162..17671)
                     /locus_tag="ACG8ZM_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491018.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(17846..19981)
                     /locus_tag="ACG8ZM_000017"
     CDS             complement(17846..19981)
                     /locus_tag="ACG8ZM_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0491019.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(20064..21719)
                     /locus_tag="ACG8ZM_000018"
     CDS             complement(20064..21719)
                     /locus_tag="ACG8ZM_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0491020.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(21721..22035)
                     /locus_tag="ACG8ZM_000019"
     CDS             complement(21721..22035)
                     /locus_tag="ACG8ZM_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0491021.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            22205..22465
                     /locus_tag="ACG8ZM_000020"
     CDS             22205..22465
                     /locus_tag="ACG8ZM_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0491022.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            22479..22793
                     /locus_tag="ACG8ZM_000021"
     CDS             22479..22793
                     /locus_tag="ACG8ZM_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0491023.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(23523..24077)
                     /locus_tag="ACG8ZM_000022"
     CDS             complement(23523..24077)
                     /locus_tag="ACG8ZM_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0491024.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(24203..26132)
                     /locus_tag="ACG8ZM_000023"
                     /pseudo
     CDS             complement(24203..26132)
                     /locus_tag="ACG8ZM_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(26438..27154)
                     /locus_tag="ACG8ZM_000024"
     CDS             complement(26438..27154)
                     /locus_tag="ACG8ZM_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0491025.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(27155..28978)
                     /gene="glpO"
                     /locus_tag="ACG8ZM_000025"
     CDS             complement(27155..28978)
                     /gene="glpO"
                     /locus_tag="ACG8ZM_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0491026.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(28992..30488)
                     /gene="glpK"
                     /locus_tag="ACG8ZM_000026"
     CDS             complement(28992..30488)
                     /gene="glpK"
                     /locus_tag="ACG8ZM_000026"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0491027.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(30624..32084)
                     /locus_tag="ACG8ZM_000027"
     CDS             complement(30624..32084)
                     /locus_tag="ACG8ZM_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0491028.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(32074..33369)
                     /locus_tag="ACG8ZM_000028"
     CDS             complement(32074..33369)
                     /locus_tag="ACG8ZM_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0491029.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(33701..34378)
                     /locus_tag="ACG8ZM_000029"
     CDS             complement(33701..34378)
                     /locus_tag="ACG8ZM_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0491030.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(34408..35499)
                     /locus_tag="ACG8ZM_000030"
     CDS             complement(34408..35499)
                     /locus_tag="ACG8ZM_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0491031.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(35600..36034)
                     /locus_tag="ACG8ZM_000031"
     CDS             complement(35600..36034)
                     /locus_tag="ACG8ZM_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0491032.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(36618..38075)
                     /locus_tag="ACG8ZM_000032"
     CDS             complement(36618..38075)
                     /locus_tag="ACG8ZM_000032"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0491033.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(38072..39967)
                     /locus_tag="ACG8ZM_000033"
     CDS             complement(38072..39967)
                     /locus_tag="ACG8ZM_000033"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0491034.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(40025..41800)
                     /locus_tag="ACG8ZM_000034"
     CDS             complement(40025..41800)
                     /locus_tag="ACG8ZM_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0491035.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(41850..43346)
                     /locus_tag="ACG8ZM_000035"
     CDS             complement(41850..43346)
                     /locus_tag="ACG8ZM_000035"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0491036.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(43540..45162)
                     /locus_tag="ACG8ZM_000036"
     CDS             complement(43540..45162)
                     /locus_tag="ACG8ZM_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0491037.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(45490..46803)
                     /locus_tag="ACG8ZM_000037"
     CDS             complement(45490..46803)
                     /locus_tag="ACG8ZM_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0491038.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(47048..47272)
                     /locus_tag="ACG8ZM_000038"
     CDS             complement(47048..47272)
                     /locus_tag="ACG8ZM_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491039.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(47292..47714)
                     /locus_tag="ACG8ZM_000039"
     CDS             complement(47292..47714)
                     /locus_tag="ACG8ZM_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0491040.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(47856..48566)
                     /locus_tag="ACG8ZM_000040"
     CDS             complement(47856..48566)
                     /locus_tag="ACG8ZM_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0491041.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(48584..49081)
                     /locus_tag="ACG8ZM_000041"
     CDS             complement(48584..49081)
                     /locus_tag="ACG8ZM_000041"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0491042.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(49118..49570)
                     /locus_tag="ACG8ZM_000042"
     CDS             complement(49118..49570)
                     /locus_tag="ACG8ZM_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0491043.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(49705..50310)
                     /locus_tag="ACG8ZM_000043"
     CDS             complement(49705..50310)
                     /locus_tag="ACG8ZM_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0491044.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(50760..51380)
                     /locus_tag="ACG8ZM_000044"
     CDS             complement(50760..51380)
                     /locus_tag="ACG8ZM_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0491045.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(51382..52251)
                     /locus_tag="ACG8ZM_000045"
     CDS             complement(51382..52251)
                     /locus_tag="ACG8ZM_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0491046.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(52274..52681)
                     /locus_tag="ACG8ZM_000046"
     CDS             complement(52274..52681)
                     /locus_tag="ACG8ZM_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0491047.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(52700..53989)
                     /locus_tag="ACG8ZM_000047"
     CDS             complement(52700..53989)
                     /locus_tag="ACG8ZM_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0491048.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(54016..54318)
                     /locus_tag="ACG8ZM_000048"
     CDS             complement(54016..54318)
                     /locus_tag="ACG8ZM_000048"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0491049.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(54343..56436)
                     /locus_tag="ACG8ZM_000049"
     CDS             complement(54343..56436)
                     /locus_tag="ACG8ZM_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0491050.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(56925..57689)
                     /locus_tag="ACG8ZM_000050"
     CDS             complement(56925..57689)
                     /locus_tag="ACG8ZM_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0491051.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(57800..58669)
                     /gene="mdcB"
                     /locus_tag="ACG8ZM_000051"
     CDS             complement(57800..58669)
                     /gene="mdcB"
                     /locus_tag="ACG8ZM_000051"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0491052.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(58694..59770)
                     /locus_tag="ACG8ZM_000052"
     CDS             complement(58694..59770)
                     /locus_tag="ACG8ZM_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0491053.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(59794..60387)
                     /locus_tag="ACG8ZM_000053"
     CDS             complement(59794..60387)
                     /locus_tag="ACG8ZM_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0491054.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(60391..62067)
                     /gene="mdcD"
                     /locus_tag="ACG8ZM_000054"
     CDS             complement(60391..62067)
                     /gene="mdcD"
                     /locus_tag="ACG8ZM_000054"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0491055.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(62057..62356)
                     /locus_tag="ACG8ZM_000055"
     CDS             complement(62057..62356)
                     /locus_tag="ACG8ZM_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0491056.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(62458..64104)
                     /gene="mdcA"
                     /locus_tag="ACG8ZM_000056"
     CDS             complement(62458..64104)
                     /gene="mdcA"
                     /locus_tag="ACG8ZM_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0491057.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(64111..65022)
                     /locus_tag="ACG8ZM_000057"
     CDS             complement(64111..65022)
                     /locus_tag="ACG8ZM_000057"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0491058.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            65142..65999
                     /locus_tag="ACG8ZM_000058"
     CDS             65142..65999
                     /locus_tag="ACG8ZM_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0491059.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(66186..66548)
                     /locus_tag="ACG8ZM_000059"
     CDS             complement(66186..66548)
                     /locus_tag="ACG8ZM_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0491060.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(66737..66919)
                     /locus_tag="ACG8ZM_000060"
     CDS             complement(66737..66919)
                     /locus_tag="ACG8ZM_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491061.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            66894..68333
                     /locus_tag="ACG8ZM_000061"
     CDS             66894..68333
                     /locus_tag="ACG8ZM_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0491062.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(68340..70892)
                     /locus_tag="ACG8ZM_000062"
     CDS             complement(68340..70892)
                     /locus_tag="ACG8ZM_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0491063.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(71006..72337)
                     /locus_tag="ACG8ZM_000063"
     CDS             complement(71006..72337)
                     /locus_tag="ACG8ZM_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0491064.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(72303..73781)
                     /locus_tag="ACG8ZM_000064"
     CDS             complement(72303..73781)
                     /locus_tag="ACG8ZM_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0491065.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(74086..75921)
                     /gene="tdc"
                     /locus_tag="ACG8ZM_000065"
     CDS             complement(74086..75921)
                     /gene="tdc"
                     /locus_tag="ACG8ZM_000065"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0491066.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        21782 a        16166 c        13053 g        25161 t
ORIGIN      
        1 aaaaactatt cacttggcag gacctcctac taaatcagcg acaaaacgtc ggctgaaaga
       61 cttatttttt tatttttctt ctaaactttt ttctacctca atcccccaag aatctttctt
      121 gaagtgattg atgatcaatg gaataacgaa catgaccact gtgatagctg ctactaataa
      181 tgcatcactc aatgtcgctg tacctaatgt agcaaatacg acaacgatga tagagaagat
      241 cagcatgcag ctgactaccc aagctaatcc gtttccgttt ttaccaattc ggtaaggacg
      301 ttcgaattct ggatgtttct tacgcatttt aagcaatgaa acagcaatca aaatgtacac
      361 gatacaataa ataacagttg tagcatttgt aagtgtcaag aatacagcac ttacgtcatc
      421 cattacacca taaagcaaag caaataatga aataacgatt gattgtgtta gaacgacatt
      481 tctagatacg ccgtatttgt tttctttatg gaatccgaat tttggtggaa ggaatccttc
      541 acgagctact tggatgattg tttttgatgg tccagtaacc caagctgata attgtaaaag
      601 cacaccaata aataccatga agctgaagat atttccgatc acttctggta agcctaggat
      661 ttgacagtca atcaagatag gctgcgtgat atttgctaat tccatttttc catctggtac
      721 gacatttgct acaagcatcg cattgatgac attcaataaa actaatccga tcaaagcgat
      781 aaatacgcct tttgtataat tttttgttgc gtctttcaaa cgaggcatgt aaacagaact
      841 catttcgatc ccaacaaaaa taaatgtgat accagctaag tatttcaatg aatcaatatg
      901 ttccaagtca gggaaagctt ttgaccatga aaagtcacct agatagccgt ttgagactag
      961 tccgactttg aaggctgcca aaacacctaa aacgaacatg atgactactg ggatatatac
     1021 ccctagccat acaccgatgt tcccgccgac ttttgccata tcgaatttca agttcaagat
     1081 cgtgatgatc cagtagataa ctaaaataca acctaaaacg aaccagtgat tattgccaag
     1141 ttctacatta ccaaatgtgt ttcctaacaa tggtcctaaa gtagaagcga ccattaccat
     1201 acctgggaac atttgcaccc ataatagcca tgcaacgaca aatccccatt tgctgcccag
     1261 tgctgttttt acccataact gaggaccgcc ttcttgagga agcatcgtgg aaagctcacc
     1321 ggaaaccatt gatatcggac cggcaaaaaa caaaactgca aaaattgaat aaaaaatcaa
     1381 tgtccaaccg actgcggcta acgtaggaat actccgaacc gttgcacaca gtgccattgt
     1441 cagaccgata aaggtcccaa gacccatttt acttacttca gatttactca ccgttgacac
     1501 tctccttgat tttttggata aaataacggt gtctttgaag tagtttcagc aataaacgaa
     1561 acaattcagc gaatatttga ggggattctt tgagtttatt gcttgaaggg tttcttcaaa
     1621 gacaacctca aaacaaaggg atcgaagagc ttaggtctcc ccttgctctt cagatccaaa
     1681 catgcgtcag aaacagttag tattcaagct gttttctaac atgattacct tgcttatttt
     1741 gcttcgcttg ccaataattg gtctgcgtag attttttcca atttttcttg aagagctgct
     1801 ttgatttttt cagcatattc ttcaaagtgt tcttctttgt tcatgtatgg tgtcattacg
     1861 ctggcacgca atacagttac ttttccggca cggttccatt cttcatctga gaatcctagt
     1921 tggttcacaa attgtaatgg gctgttgcca tagtctggga tagcaaagtc agtatgtgaa
     1981 gttaagaact cattaccgta aatgcttcct ttcacgtatg aagaataatc atatacgtca
     2041 tggttcaatt tattcattgc aactaagtca tcgttgcctt tttctttgaa tacatagtct
     2101 accatattga aatctggata agttaatgga tgaacttcga tttctttgtc tcctacttta
     2161 aatgacaagt cattcaagaa gttgtagaaa cgatgtgcac cttcgattga tgcacccatt
     2221 aatttaccat atcctgtgac attcaaaggt aagacatggt gtgctgccca aacgctggct
     2281 gctgttgccc ctgcttttga accttccaag atataagccc caagtaatgc tgggatatct
     2341 gcgccttttt caaatacata tgtcgcaaag taagagataa cgtctctcat gcggatatct
     2401 ttgatgacga taccaccggc agaatatgga acatagccca ttttatgtgg gtcaatcgtt
     2461 acagattctg cttcttcgat tgctttatat gcactgtgta cttcttctaa gatataatct
     2521 ttgttttctg taaagacatt gtatttgtaa tgaacatctt ttaaatcttc aaatggaatg
     2581 aagttgttgt cttcgtctaa gaaaatcgca cggccgtatc cgccataagc agcatctacg
     2641 tgtaagtaga agtaaatacc gtctttttct aatacgcgac gcaatgcaac gattttatcg
     2701 ataccgtcga ttgctccttc ttctgttgat cctacaacac cgactacacc taggattggt
     2761 gttttttcag ctgctagacc acgaacgatt ttttcaagtt cgttgatgtc catgcgatag
     2821 ttgtggtcaa ctggcactgg aatcacttgg tctaaaccga taccgatgat gtcggcagct
     2881 tttagccatg aatagtgttt agtttgtgga accaaccatt ttcctaattt ttctaaatgt
     2941 tttccgctac gtgctgagtg agctttgatt tcgtcgattt tttctgggac gctgtctaac
     3001 agattcatga tttcttctgt tgacaagttc attaattccc aatcgctttt tccagcaact
     3061 agttcaggtg tcacttcttt cattgcaagc ggcaatgatt tgatgttgcg ggcataccaa
     3121 agtccttcta agtttgctaa agaaccatca gcaacgatgt ggccccagcc atctttatag
     3181 ctcattaatt ttgcaaattc cattcctact tcttcttcca tttggcttgt tgcaggagaa
     3241 gattcatagg caacgttatt accattccat agcatagcaa aattgtacgc tagtaaagat
     3301 ggcatcaatg tttcagagtt catatgaccc cagtaacgtc cggcattatg ccaaggaacg
     3361 gaatgggtac gcatccgcgc tgaaatttct gatagaacat cctttgtttt attcacagtg
     3421 tgttcaaaac tagcactgct tttttcttct ggcgtaataa taggcatgtc ttgaggcatg
     3481 tagttttgac gccagcctaa atgttcatcc accaattcat tcaacaaagc tttatagatt
     3541 tggccgttct cagctttgtc cccaatgaat agggcattta ggtttagatc tttcgacaat
     3601 gattcactca tagtgaaacc ctcctaaaaa aatatttaaa ttttgtcgag ccctacatta
     3661 cacctgttct tctattctgt aaactaagtt ttggcctgat ttttaatcat attttaatat
     3721 gtatcaatta tccgaattgt tcacactttt gaaataatta aaaaaaacga gggaatttac
     3781 aaatgtaaac tccctcgcaa agattgtatt aacgacaacc agcgttctat tttaggatgg
     3841 gtttcaaaat tggaatgttg taatagtatt tccctttttc aaggtaaaaa cttactaaat
     3901 tctaatcaat tactttggcc aaaaagtaat ttttcttacc ttttcgaata accacaaact
     3961 taccatcgaa ttcatcggat ggatttatga caaaatttaa atcggtaacg cggtcaccgt
     4021 taatacttat agctccgttt gaaacatctt cacgtgcttg acgtttagat ggttcaattt
     4081 ttgtggaaac aagcagttcc acgatatttt caggtgtgct tgaaatttca acgttcggca
     4141 ttttaccgaa tccttgcgcg atttcttccg catttagttc tttaatattt ccagagaaca
     4201 atgcttgcgt tattttttgc gcttctttca agtcttcttc actatgcaca aatcttgtaa
     4261 cttcttctgc caaacggcgt tgtgcttcac gtttctcagg ttctgtctct acttttttcg
     4321 ctaagtcttc gatttcttct tgagacaaga aagtaaagaa tttcaagtat ttcactacat
     4381 cgcgatcatc ttgattcaac cagaattggt agaactcaaa tggtgaagtt ttcttcggat
     4441 caagccaaac ggcaccgcct gcagtttttc cgaatttcgt tccgtcagcc ttcagcatca
     4501 atgggatcgt taaaccgaac gcttctgctt ctggtccttc taattttcgg attaggtcta
     4561 atccagctgt gatatttccc cattggtctg ctccaccgat ttgcaattgc acatcgtatg
     4621 ttttatgcag atgcaaaaag tcgatagatt gcaggatttg gtaagtaaac tctgtaaatg
     4681 agattcctac ttccaaacgg ctggcaacga tatcttttgc taacatcgtg ttgatgttga
     4741 agtttttacc gtagtctctc aaaaattcaa gtaaagagat ttttgacaac cagtcgtagt
     4801 tgttcacaaa tgtgatattt gcatctttac caaacaattt tctcatttga ttggaaagtg
     4861 cgtccacgtt atgttgcact tgttccatcg tttgcaacac acgttctgtt ttgcggccgc
     4921 ttgggtcgcc gatcgttcct gttccgccgc caatcagaat atatggatga tggcctgcca
     4981 attggaatct tttcatcatc ataaatggaa tcaaatgtcc gatatgcatc gaatcaccag
     5041 ttggatcaac gccgcaataa agtgaaatgc tcttttcttc aacgagctcg cgaagtcttt
     5101 cttcgtttgt ttgctgatta atggcgtcgc gccaagttaa ttcatcaata atattcattt
     5161 tactgacctc cgtgtagttt ttttatgaaa gcaggaacct gctagcatgt gtaattaatt
     5221 atttcttcag gataggatag gaaataaaaa aagtcccttg gcatataagc caagggacga
     5281 aatctcgcgg taccacccaa tttgtatgtc tagatatcca acatacctct cgaacattta
     5341 acgctgtcgc gtggcaatca ctgccaaaga ctcctgaaat gtaattcgct ttaaagcatg
     5401 tactggtttg cagcgaccac cagctttctg aaacattggc tttaacaact acttcgtttc
     5461 gatcttcgtc ttgttattat taatatgtgt ctatcataga gcgcaacttt ttctttgtca
     5521 acagaaaaag gatatttttt ttaaattcct cattttttct tttttgaatc cgctctaatt
     5581 ccgaacattt tattttcaat tattgttaaa agaaataact ttttctgtat tttttctgca
     5641 atttatcgag aagtcacttt actttgcctc ttattctttc ttcgttacaa taaggaatgg
     5701 gacaagtaaa aaaatgaaaa agcaagggac gaggtatatg gaaaaaaagc aaaaaattat
     5761 tttaggagcc acttctgctt taactatcgt tattttaatt ctaagtttgt tttttgtcat
     5821 ccgatcatct acaaaaaaag aaccaatcat tcctgaaaaa aaaataacag aaaaagcaaa
     5881 agaaccgaca gatgataagc aacaactagt tactcaagca aaaaatctgg ctcaaagcta
     5941 cgattatgac caagcgattg cgttattgga aaaagatagt aagaacggat ctactgaaac
     6001 acaacagctt cttcaaacac taaaaaagga gaaagaacaa ctgatcgact ggtctgatcc
     6061 tactcagatc tctcatgtct ttttccacag cttgatcgtg gacccgcaaa aagccttcca
     6121 ctctcaacag gcacaaggat acaaagatta tatggtgact gttgaagaat tcaaccgttc
     6181 gatcgaacaa ctttatcaaa atgattttgt tttagttaat ttgaatgatt tgatccaaaa
     6241 agacgaacaa ggaaatcttt cctttaacgg gcttgcgcta cctgaaggaa aaaaaccatt
     6301 ggttctttca caagatgatg tcagctatta tgaatatatg gataattccg gttttcctag
     6361 ccgattggtc gtatcaaata acaatgaaat caaaaatgtc tataaagaga aagataaaga
     6421 agaaatcggt gactttgaca tggttcctct gatcgatgcc tttgtcaaga aacatcctga
     6481 tttttcttat cgaggcgcaa aaggtacctt ggccctgaca ggttacaacg gagttttagg
     6541 ttatcgaaca tcaaaatctc aatatggaga caatgaaaaa acaaaaaaag agattaaaga
     6601 agctaaaaaa gtggcggaac agttgaagaa agacggctgg agctttgctt cccatacatg
     6661 gggtcacttg aatatgactc aagcttcact agaagatgta aaaaaagaca atgaattgtg
     6721 gcagcaagaa gttgcaccta tcctaggaaa aacaaatatt ctgatttatc catttggtgc
     6781 agacatcagc gactggcagc cttattcagc agaaaatcaa aaatttgctt acttgaagag
     6841 tcaaggtttc gatattttct gtaatgtcga tgcttctaca ccagcttggg gacaattagg
     6901 tacggatttc tatcgaaatg ctcggatcaa tatcgatggc attcgttttc aatctgattt
     6961 agacggaaaa aatcctattc ttgatccatt catcaatgtc catgatgtct atgacaaaga
     7021 agcacgcgaa tctgcttgat tttacgtcat ttatgaacca aaaaagccga actgacattt
     7081 tatcgtcagt ttcggctttt ttatgcttta tttcttcttc ggtgcgattc ccttgatcag
     7141 gacttctgtc cattctgctg tatatggaag cagttttgcg tatatctccg gcttagaaat
     7201 tccttctacc attgcttgaa aatagaggat caagaactca tccgaatatt tcaaatccac
     7261 ttttccttct ttccgccctc gatggaaaag atcaagcatg atactgaaac tttcttgcgt
     7321 atacgcatcc atcgcgtcag aaaattcttt tttttctttt tttgtatagt atgtcatcaa
     7381 atcgatataa aattgtttgt tcactcgatt gagataacgg attttgtttt cactcatggc
     7441 aatcagtgtt tcttcaaatg gaagattttc cgcaatcaac gctttcgctt cttgccctaa
     7501 atcattcata aatgcaaaaa atacttcata aatcagccct tccttgctgc caaaatattt
     7561 aaaaatcgtc gctttactta cctgagcgtt ctcagctacc ttatccattg tcactccagc
     7621 ggttcctatg tctgtgttca tcaagtcaaa agctgcttgt aaaatctctt tctttttctc
     7681 ttgtgctctc ttttcaaaac cgttcatttg tagagcctcc cgtaatttta aacttttata
     7741 atatatatag ttcaaattta ttctgaggtt tattataact atctattgag aaagtatcaa
     7801 gctagcaatt ccttgtttat gaattcattg acagaaattt ataaaaagaa tattatgtac
     7861 taaaataata aaaaaaagtt ttataaaaag gtataaaacc tttttattaa actatttaat
     7921 cttgtttagt atatattttt agaaaaagga gtggggtttt tatggcagaa atcgtcaaag
     7981 tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg
     8041 tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg ttctggaaaa tccacgacga
     8101 ttcggacact tttagggatt ataaaaaaag acggcggaac ggcaacgatt tttgggcaag
     8161 atgtatggga agatagttta tctatccatg aacgtctttc ttatgttcca ggcgatatct
     8221 ctttgtgggg aaatttgact ggaggagcga tcatcgatct cttcatgaag cttcatggaa
     8281 aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt ctctcttgat cctaagaaaa
     8341 aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg cttgattgct gctttggctg
     8401 ttgattcgga tctctattta tttgatgagc ctacttctgg tttagatcct ttgatggaaa
     8461 cgattttcca agaagaagtt gaaaaattga aagaacaggg caaatccatc ctactttctt
     8521 ctcatattct cagtgaagtc gagcgactag cggataaagt cgtgatcata cgcgaaggaa
     8581 cactcgatga agaaggaaca ctcgatgaat tgcgtcattt gactcgttcg accatcacta
     8641 tagaaacaga agcagagaat agtcacttag cacaattgaa cggtgtccat gactttgtgc
     8701 aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc tttcgatgtg attttagctg
     8761 aagcgtccaa attaggtgtg aaaaaatttg aatctattcc accgactctt gaggatttat
     8821 tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac tgataaattt tcacgctggt
     8881 ccaccctttt ttctcaaaac cttagccgtg actggaaaaa gatcctgata tggctagtcg
     8941 gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga agaattgact tccggacaag
     9001 gattagctgg attatacgaa accttgcaaa acccggcaat gattgcaatg gtcggaccaa
     9061 ctccagcttc gaatgctgct gagtatactt taggagcgat gtatgcaaat gaaatgttgc
     9121 ttttttgcag tttgtttgct gctgtgatct ctgttttaca cgtggtgagc caaacgagaa
     9181 aaaaagaaga acttggtctg attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa
     9241 actctttagc tgtcatcgct gaaagtattc tgatcaattt gcttttagca atatcgatca
     9301 gctgtacgat gatttttttc caagcggatt cgatcactgc agaaggtgct tggctttttg
     9361 ggctatctgt aggtagtatg gggattttgg ggagtgccgt tgcactgttt gctgcacaac
     9421 tgatgccaag ttcagccagt tcgaatggtc tgtctttagc gtttttaggt ggactttacc
     9481 ttctacgagc aataacagat gtagctgatt cctccctttc gatgtggaat cctttaggct
     9541 ggtcttattt gacctatcca tttacaacta acaactggat tcctttgctt tatttgtttt
     9601 tgttcagtct aatcttgata atcagttcat ttgttttaga ggaacatcga gatttgaaca
     9661 ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa acgttctctt ttatccttgc
     9721 gtggtttatt attctatcta aataaaggga tgacaatcag ttggcttatc acttttggtt
     9781 tgatgggggc ggcatacggc tctatctacg ggaatatgca acagttttta gaaagtaacg
     9841 acttaatgaa acagatgttt atccaatctg gcgtctcaat tgaaaaaaac tttacaagca
     9901 cgatcatggt agtcttgatt tgtctagctg ctattttacc aattgctagc atcaatcgtt
     9961 tatttacaga agaaacccac ggacgtatga atcagctgtt cgcaacaaaa atttctagga
    10021 accaacttta ttggaccaat atgttactag ctattctatt tgctatattg gcaatcctga
    10081 ttagttctgg tgcactaggg ctttctgctt tggcttcgat gaaggatgcg aaagacatgg
    10141 atctattgat gtttcttaaa gccgggttca atttcctgcc tgccgtttta gtgtttatcg
    10201 gaattagtgg ctttctattc ggatggcttc ctcgaatcgg taaacttgct tatatctact
    10261 tagcttactc ctttgcactc aattactttg gcggaatttt ggatttacca gaatggtttt
    10321 ccaaaacagc tgtccctagc tgggttccac ggatgccaaa cgattctttt gatctttccg
    10381 tgttccttac gttatcatgt atcagccttg cactgttcgt ccttggttct attggttata
    10441 ggcgaagaga cttactagaa ggataataaa aaagcatgta caatttcatg ctagcctcca
    10501 aatctgggtc aaaaatccaa catttggagg tcattttttt atttacaagt cttctaactg
    10561 ctctttatca atgatccaga cttctcgatt ctttcttttt acataaccat tctcctctaa
    10621 taatttaaat tttctagata aagtttcggg ggtcgtaccg ataaatgcag caagttcttt
    10681 cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct ttagctaagt caagcagata
    10741 gacagcgact cgttcttcta ctttttccat catcaaaaaa gttgtctgct gctgtacttt
    10801 tgttaatttc tcaacgttga ttgctaaaag tttcagacta agggcgggat aatgcagtaa
    10861 gagttccgag aaatcttttt gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc
    10921 aaataaagac tcatttggaa cgcctagcaa ctggctctct ccctcatatc cgcccggctc
    10981 tattactcgt aaaagctgtt cctttccgct agcagataat tgatacactt tcatactgcc
    11041 ggaggccacg atcactaatt gaggttctcc attcggcgca agaatctgtt ctcctttttc
    11101 cacatagtga tgtttgacaa gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt
    11161 gaacaaagga acaaatgaaa cacataaatg atcatgactc ataatctctc ctccttctaa
    11221 acagctattt ctaataaaga aacataactt cccttattcc aactgataat tttcttttat
    11281 catagcataa acgtcaagcg aaccagatac cagcaaatag agtggatttc tttttgtccc
    11341 ttgctttatt ttccttcttt tgagacctta agtaattgat tcctatcaat tttttaaata
    11401 tttacttttt gaataatcaa aaacaaacct tcactcgcaa gccgagagta aggtttgttt
    11461 ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc gcacctagct tttgatatag
    11521 tggtgctagt tcttgtaagg tttcagcggt tatttgctca acgtgttcga tttcttgcgg
    11581 atagaagaat agtttaccca tttgaaaggt tcggtctctc atcgcttttt tcaactcttc
    11641 ttgtttcttt gtatcggaca aatagtcaga caaagctaag tggtcgtcaa gttcatgttc
    11701 tggttgcggc catatgtaat aatccttgat atcgactgtt ttgctccact ctttcagtat
    11761 cgcaagcaac tggttatacg ctgcaatgct accatatcgt tcttcgcttt tataatgttg
    11821 gtacatcaaa tagatttggt attgtttttg attcaacaaa actgctagac aggcattctc
    11881 gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt
    11941 cgtccagctt tcgattttag gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt
    12001 ctggagctga atctctttcc atttttgcca gtgttcttta tattctttct tgatttgttc
    12061 aatcttatct gttggatggc tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa
    12121 caactgataa tctgattttc caagttccat tgtttcgcct ctttccgtta tcttttgtat
    12181 tctacctgaa ttttgcgata tggacaaata gaggttgtga aaaagctgtt ctgcatcaag
    12241 tcataagaac agccaaacaa aaaactgcga tgagcatcac cgcagtttta ttgaacttaa
    12301 ggttgattga tcacatctaa aagcgttgaa aataaaatgg cactagaagc agctcctggg
    12361 tcgatatgtc cgattgcacg ctctcctaga taggaagctc gtcctttttt agctaccaag
    12421 actttggtgt gttcttttgc ctggtcaatg atttcttttg ttaaattccc tgccttcaat
    12481 gcctctgtga ctggcagcca aacatctaac atcgttttat cgtcagcttc tgcctttcct
    12541 cggctttgaa tacccgctgt tccctgctcg atcacttcgc ctaatttttc ttgagaatca
    12601 atagaatcga cctcttttgt cgctttcatc atgttcatga atgcagatcc gtataacgga
    12661 ccagaagccc caccgacttt cgatatcatc gccatagaga atactttgaa tgtatcgctg
    12721 attgtttcag gatgatcggc ttgaaaagct tcttcataag ctgaaacccc tcttcccatg
    12781 ttatttccat ggtctccatc gccaatcggg gtatctagat cactgagata ttgtttgttc
    12841 tgattgattg cctctgcaaa ttgcgtgagc cattcttgga tttcttgaac ggttagtttc
    12901 atattatcac ccttttatct tgagattttt taccatgaaa tcgtctgtac attgctttct
    12961 aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg ttagagaaag accttgcata
    13021 tctagtgagg tcataaaatt accgactttt cggaaaacta cattgacgcc tctttcttcc
    13081 aacagagcta aaacatcatt catgaacacg aactgttcca tcaaaggcgt cccgcccatt
    13141 ccgttgacta gtacacctgc ttcttttggt aaattcgagt aatcatctaa aatcttagta
    13201 acaagttcct ttgccagtac tttagaaggc tgcatctttt ctcgacgata gcctggctca
    13261 ccgtggatac caacaccata ctcgatctca tcttctccta gttcgaatcc tggtttccct
    13321 acttctggta cagttgctgc tcgaagcgct agaccgatcg tcttagttgc ctgtacgacc
    13381 ttttctccca gttctttcaa ctcagctagg gaagcaccgt ttcttgctgc atcgcccacg
    13441 attttgtgta caaggacagt acctgcaact ccgcgttttc cagcagtata cgtactattt
    13501 tcaacggcga tatcatcatc tacgatcaca ctttctactt caatatcgtc catagcagct
    13561 aattcctttg ccatatcgaa gttcaatgca tcccctgtat aattcttaac gatcagcaaa
    13621 actccttttc cactatcggc ttccttaata gccgtctgaa tctgatctgg agttggagaa
    13681 gtaaacacat caccaagcac cgctgcactt aacatccctt cccctacaaa cccggcatgg
    13741 gatggttcat gtccgcttcc tccaccagaa accaaaccaa cttgttccgc tttctgattc
    13801 ttggctacaa cacgggaaga ttctacacga tgaacaagtt ctggatagct ttttactaat
    13861 ccttccagca tttcttcgac aatattgctt ggatcattca tgattttttt caatttattc
    13921 ccatcctttc tttccggcat tttgtgcttc tgttaagatt tgctgagtat catctgttat
    13981 accggcagtg atggctgctg caaaagctcc ttccactaaa ggcgcatcca ccagatgata
    14041 acgttgctgc tgctcttctt ccaacatgtc ttttgccagt tccgagttca ataccgcgct
    14101 accgatatcc gcaaaaacaa aataactagc tgcatcgctt gattcattga ctgcttctac
    14161 gattttcatc gggtctgatc cgatctcgcc atttattccg ccgagagaaa aaatttgcac
    14221 ctcttcggat ttttgcatct gctcaatcat ttctttaata ccatctgtta ctttttgact
    14281 atgagaaata agcaaaatac tttttttcac aagaatcttc ctttctttta catagttgct
    14341 aacgattggt ttcgctttca ttaatgaaca attcaagtat ataataaatt tctaattttg
    14401 ggaaaatatg tgtagtgctt cttgaagttt gttatacttt aattaaaaga gaggagtgat
    14461 cgaatgatta caacaacaac gaacagtgta gagggaaaag ccgtagtagc ttataaaggg
    14521 atcgtttttg gtgaggtcat tactgggatc catgcattta aagacttgga ggcggggttg
    14581 cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg aactaatggg tgcaagagaa
    14641 gaagcacttg ccgaaatggc cgaacgtgca aaagcattag gagctgatgc gatcattggc
    14701 gtaaaaatgg actatgaagt attaggcagc gacaatggca tgctgatggt cacatgtagt
    14761 ggaacagctg tctccacgag ataagtaggg gacatctgtt ttaataacta aagagagatg
    14821 ggacatgtag tggcactcaa gaattagatt tttcagtcta acttcgaggt actctacctt
    14881 cccatcctct cttttttatt tctttttgcg gatgattccg taagctgctg cgactaacac
    14941 acccgcccct gctaaggaat aactgacaga agctgttttt tcatttgttt ttggcaaagt
    15001 ctgacttgtt gatttggatg gctctgtcgt ttgctgaact gtttctgtcg tgcttccaga
    15061 ggtcactgat ttttctcctg ccgaatgcaa aatatctttt attcgttgga aactagctgc
    15121 taagtcagct ttttttgctt caaaatcatt agcaactgtt tgtgcttcgc tgatctttcc
    15181 gtggattgct tgactggccg ctgtgatatc cgcttgagtt tgacttagct ttccagaagc
    15241 tgtttgagca acctgattta attgctgatt gaattctttt gcttgttgga tcttcccgtc
    15301 aagatctgtt tggacagctt ccgcttttgc tttcgcttcg gcaagctttt tctcaagttg
    15361 tgcacggtca tttcccactt tatttttaag atcagctaaa tcttgatcga ttgctgcttg
    15421 gtcttctttc aatttgtttt ttgcagcttc taatgcttct tcgagttctt ttcttttatt
    15481 ttccaagtct tgcaaatgat cgatttggtc agaagaggta gaactattta ttgatgtatc
    15541 ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt acattggaag tcatttctgt
    15601 ttccgaggca ccgttttcag caccagaaga gcttatggtt gttgaaggtt ctgtttgagc
    15661 aggttcagaa gtttccgcaa aagctgtctg tcccaataag aaaatactgg aaacagcaat
    15721 tggccagatt aacggcttca ttcttttttt catcactttt cccatcctta ctacttttta
    15781 actaatttct tattataagc aaaaatgttt ggaagaacca taaacacaac aggctgtttg
    15841 tctaaaaaac ggtgcatctt ttattttttt gtacgttccg ggcttttcca tgtcccgctt
    15901 ccataaccta aaatcgtttt gactgctgga atgaaagtcg tcacgatcgt tatcgcgttc
    15961 actatccaga acaaaagcat atatagtggt gcgaaaagaa ggtatttcaa ttttcttcgt
    16021 cgatcatcta caatcagcga agctagcaac tggaaaaatc ctgcgaccat ttcaaagcag
    16081 atgaacacaa aactgaatgt aaaagtaata taaatctgtt cgtagtttcc ttgatataca
    16141 taatgcccga ttaatccagc aaaaagaact acggaaatac aaaagaatat tgaccagaca
    16201 atactcaatg tttgatcaat gaagatgatc gttcttccaa tatgtttaaa aggatggagc
    16261 atcacttttt tgaaattcgt cagccagact tctgttccac cttttgccca acgttttctg
    16321 tgtcgatata gcatcttcaa cgtcacaggt acttccatga aaaagataat ctcaggagca
    16381 aaaacagata accagtcatt taactgatga tcccaagcaa tactgatgtc ttctgtcgct
    16441 cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt ttcgatacat cgtatttgct
    16501 ccactgtacg cataaagacc accgaaaaca gctgactgtg ttcgtttaat gatgcccacg
    16561 atacttgaga attcaaccgt ttgagatttt gcaatcaatt tggtacgatt ttggacatcc
    16621 atatttgcag tgaccgcagc aatatgtctt gcaccagggc gaatgaaata attcacatac
    16681 ctaatcagtg catctggctc cggtaccgtg tctgcatcat tgcttaaaat caatttccct
    16741 tttgcaaagg caagtccgat attaaacgcg tgggcttttc ctttattttt ttcgatccga
    16801 accacacgaa gattagcgta ttttttcatc agtctagcta atatttctgg tgtttggtcc
    16861 gttgaaccat cgtctgtcac taatacctca tagttgtgat aattcaattt cgtcatcaaa
    16921 tactcgattg tatattcgat cacaatttct tcattatggg ctggcaccat gatcgtgatg
    16981 aacggttcga cactcaaggg aacatctacc cattcttttt gcttatgttt gtataaaaac
    17041 acataacacc agacaccaat aaaccataca aatccgccta aaatcggata agaaacgaga
    17101 aaaaaataca taatagacaa acaagtatct aagaataaat tacctgtatg ggtgattgag
    17161 atcatttcag ttccacccca ttttctttat acaaatctgc aatgaatgtc gtatctagat
    17221 ttttctcttc ggagacagaa taaaaacgga ccgtttctct ctcttctttt gttccaaaac
    17281 gttccgtata cacttcttca agtaattctt gtcttttttt caatttttca tcttcatatt
    17341 caaaagattt atttactttt ttgtatagat ttcgattgtt ccacctagtc aatacaaatg
    17401 aggtaatcag gaaactgaaa aaaatcaata caaaaaagcc gattaagaaa tacagcattt
    17461 tcttttctga taaaaaagcg tacaaggaaa aatccttggc tctttctggt ctcgttaatg
    17521 ggaaaaagac ccatataaaa ggaagaacaa cgatcatcca tcctaaaaga gccactaacg
    17581 tttgccaaat ctttaaccag taattacctt ttgaaaaata cttatctttc accaaaattg
    17641 atgcaacttc ttctcttgtt acttttttca tatcctcacc tcacgttttt ttgttacgac
    17701 gtaggagttt accatacatt tcatttcttt tcacataaag tgttcctaaa tttttcctaa
    17761 gaaaaagaac aaaacatttc tcatcagcaa atgaaaaata ttttgttctt tttatcagtt
    17821 gttcttttta ttttttatct cttaattatg ttgctctttc caatacgaca atgcttttca
    17881 ttcctctaga tatcgccgtg tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt
    17941 caaatggata taagattaca tgatcaaatt ccagtccttt tgctagactc aaaggaatga
    18001 tttggcagtt ttctaatcca tatgaatttt ttttaatcga ttcttgtagc gtttgggttt
    18061 cctcttctgt ttgggtgata actgccgctt tttctccttt tagagaaacc aaaatctcag
    18121 ctagtatttt caacaattct tcagaggatc gaaaagagcg gtattcgggt tcttttcctt
    18181 gcggtcggat cgatacgatg tcgatcttct cttgatccac agcataggtt ttgaaaagct
    18241 ctgtgattgc tccgctagaa cggtaactat ttctaagatc ataccgatgg atcggcagat
    18301 ggtagtcgtc gaaacaacgc ataatatcag caaaagtagt ggaagaatta aagattgcct
    18361 gattctcgtc acctactaaa gtaaagtgag tttttggaaa taattcgatc aatagaccta
    18421 attgtgccag ggtataatct tgtacttcat caatcaatag atagcgaaat gctggtacat
    18481 ttaccttttc aacaagtaaa tgacgaatag ctaatataat gactgcttca tcaacagtac
    18541 gtgtgggttg ccaagcataa gctgagccat atcgtttttc ataaattctt tcaaacaact
    18601 gatgctcatc cacccatgcc atttcttcga tttgtctggt tatttttcga tatttttttt
    18661 gtaaaagttt ccttgcataa gctacaatac tttgttccga atcatcagaa atcaatttac
    18721 cgaataattc caactgccta tcttctgtca acgagagtac ctgattctgc acagcaggac
    18781 tttttgcttg tttcaaaaga tgattcttcc attcgctcat cagtgccttt ttcgttgctt
    18841 gaagcctatc gatcaattta ggatgagcag gaactttctc atatattttt tcgatcgttt
    18901 ttttagaaag gactgttttt ccatttttag taagtccacg gataaaattc ggatgatcta
    18961 aaaacaaagg atctgattgt tttagattat cgataaactt tttgctacgc aaacgttctg
    19021 tctgctccga tacattttct cccgtgatac gtttgaaata agcctcttca ccttctattt
    19081 cctctgctga aagctgtgaa accagctgta tgatggttag attcagtgga tttctttcac
    19141 ctaatgatgg taggacattc gaaatatagt cgataaaccg atgattaggt gatagaatca
    19201 gcaagtcatc ggatgtcatc gtatctcgat ataaatagag cagatacgca atacgctgca
    19261 tgatagcaga ggttttacca cttcctgcta cgccatttac caagatattt tgacttttca
    19321 catcccgaat gattgtgttt tgttccgatt ggatcgtagc agtgatagcc ttcatttcat
    19381 tggtctcatc ttggcctaag acttctaata atacgtcatc ttgaatagct acagacgtat
    19441 caaaaaactt gaccaaacga tctctttcca aaagaaattg tcgtctttct ttaataaccg
    19501 tatcgatttt attttgacga accgtataag aagatggacc aatcatgtta ttgtaaaaaa
    19561 gttcggcaat tggtgaacgc caatcataaa caagattttc atctgcatca tctgtgaaat
    19621 ttgctgtacc gatataaaag gattcttttg gctcattatc cagaaagtct acaacgactt
    19681 ttccaaagta gggacttttc aataaacgtt cgatctttgc tagctgattt tctgcgtttt
    19741 tctgtttgat attcatctga tcgatttctc tatttttcat ttcaatcatc gaaaaagtat
    19801 ccaggttatc taagtaactg tcaaaattca atcgaagctc gccagccatt tctttcaacg
    19861 actggtttcc ttctattttt gccgtatcca tcgattcccg cagttcgttt tttttatcaa
    19921 ttagcttttg atacacgttc ttcagatatg cttcttctaa ctgtttttgt gtgttgacca
    19981 tacacattct cctctttttt taaagtggtg gaaccgatta gtttacctct tttaagagtc
    20041 gaatgcaagc tttttaacga gtatcatttg ttaggatata tcagttcttc aggtcggaca
    20101 gtttgatata aagaaaatgc accatcaagg ttttttacgt ggtagccaga ctgcttcaag
    20161 attctttccg ctagatagct acgtaatccg ctatgacagc tgacgatata ttcttgttgg
    20221 ctgtctaatt catttaatcg gtcccgaagt tcattcaaag ggatatgttt ggcttccttg
    20281 aatttgccct tttccaattc ttctgctgtt cgcacatcta ataaaatttt tccttttgct
    20341 agttctgtcg gcaattcatg ccattgtacg gtttcactaa gcccttctgc aatgttcatt
    20401 gctgcatatc ctagcatgtt tactggatct ttggctgagc caaatggcgg tgcatacgtc
    20461 aattctaatt ccggtaaatc gaagatcgtc aaatgccctt tgattgctgt tgctaaaata
    20521 tcgatccgtt tatccacacc ttttgcccca acaccttgtg caccataaat ctcgccagtc
    20581 tctggatgga agatcaactt cagtaaaata tctgttgctt ctggataata gcttgcatga
    20641 tcttttcctg atacatgaat gacttcaaaa gacagttcgt tttctttggc tgttctttca
    20701 cttaaaccgg tggatgcggc agtcattcca aacgttcgta taattgctgt tccaataccg
    20761 ccttgatttc ttcgagaaat tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg
    20821 gctggtgaag caagagaaat aagtgcatct tttccagtga tttgctgttt tacaacgata
    20881 gcatccccca ccgcataaat atcaggaata ttcgtttgat aacgttcgtc tactaggata
    20941 cccccgcgta tgcctaattc gattccagcg gtcactgcta gctggttatc aggacgaaca
    21001 ccaatggata aaataactaa atcagcaggg atggtacgcc catcatccag tcgcaatcgt
    21061 ttcccgtggt cttcaaatcc agcgacagca tgagaagtaa tcacttctac tccttgatga
    21121 gacaattctt tttctataaa agctgccatt tcttcatcca gtgttggaag aatatgtggt
    21181 gcttgttcga tcaccatgac ttctaaacct cttcttttta ggttttctgc catttccaat
    21241 ccgatgaatc ctgcaccaac gatcacggca cgctttggct gtttttctaa tgcatgcatc
    21301 actttatcga tatctggtac atttctaata gaaaaaacat tgtcggcttc tgttatccct
    21361 ggaatcgatg gaacaactgg ttttgcacca ggagataaaa ccaatgcatc gtactgttca
    21421 atgtgttttt gaccctcatg tacgacttct accgttttgt tttcgggaaa gatggctgtc
    21481 acttcatgat ttggacgcac atctaaacaa aaacgagcgg ataatgattc tggcgtttgg
    21541 acaagaagat tttcccgttc actgatttct cccgaaagat aataaggcaa gccgcaattt
    21601 gcaaaagaaa catacggtcc tttttcaaaa acaacgattt ctgcatcttc cattaatcgg
    21661 cgaagccgtg tagcagcaga cattccacct gctacacctc cgacaataac gattttcatc
    21721 ttatttccct cctactgttc gatcaaacca ttgattcatg ccaccacgta cattcactac
    21781 atcataacca gatttcttta attctttagc tgcttgttta ctcctcatcc ctgattgaca
    21841 aatcacatag acggtctttt ctttgtctcc atcatatctg tttatacttt gtaatggaac
    21901 atttttagct ccttttatat gcccgccccg gtattctgca ggtgttctaa catcaagtaa
    21961 cgctggattt tccaaaagct tttcctttaa ttctgggact gttatcgatt ttattgattg
    22021 aaatagcaaa ttcattttct gcacctcatt tacattttta taaatacctc atggggtata
    22081 ttaaacagga gctttattaa tatgtcaacc agaatgactc ttcatttttt tcactacttt
    22141 ttatatttgc ataaaatacc gataaggggt attatctaat tataaaaata gaggagaatg
    22201 tgttatggag attgataata aaaatatcct gaaccgtttg aaacgtacag agggacaaat
    22261 tcgtggtatc caaaaaatga tcgaagacga aaaagaatgt atggatgtca tcactcaatt
    22321 aagcgctgtt cgttctagta ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa
    22381 aaattgcttt gagaaacctg atagtaatcc agaagaacaa gcggctaaac tagaacaagc
    22441 gatcaagatg attattaaaa agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg
    22501 atgaattaga aaaatggtca gaaacaggga aaaacgtaaa aaacctgata aaagcttctc
    22561 cagaaacgac gattgtcgta tctgtaaatg gcatcgcgat taccggttat ctagactctg
    22621 ccaatgcaga attcttagat ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc
    22681 gtgctaatca tatttcagaa agttctttac ctgaacaagt aatcgttgta ccagcaggtg
    22741 tccttgattt agttgaactg caatcccaag gttatgctta tatcaagcct tagtcagtag
    22801 aggttgcgac agaagtgatc agcttcaaga aatttcggct tatttttcga agaagctact
    22861 tctggaaaac cgtgaatcaa aggttgtgaa aacgctgtcc tgcatcaagt aataagaaca
    22921 gccaaacaaa aatagcttct catatttttc gtttggttgg gcttattacc gcagatgcaa
    22981 gcttttgaac accgtttaag gaagaggttg tgaaatcagc ggtttgacct aagtattaga
    23041 gaaaaaaacg ggaaaatagc tcctcatatt ttgccgtttt ttgagctaat accgaaggtc
    23101 agctgattga acaccgttta aggaagagat tgtgaaaaag tcgcttagtt tcgagtatta
    23161 agaaaaattt tgtgaaaatg gatttctaca tttttgcata aatttggctt aataccgaga
    23221 aactggcttt tgaataccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta
    23281 ttagagaaac aaacggaaaa atagctgctc atattttgcc gttttttgag ctaataccga
    23341 aggtcagctg attgaacacc gtttaccaag aggttgtgag cctaccattt aactccgaat
    23401 cacaagaaat aatttcaaaa acaacttctc ctgtttttgg ttcggttggc ttattatcga
    23461 gaagtagcta ctgggagtct atttatcaaa aaaaggaatg cgacataggt cacacccctt
    23521 ttttattttc tttgaaacat ctttcggtac tcgacagcat acaagacaaa agattgtaaa
    23581 agaccgtaag tgatgacggc aaaaatgact ctcacccaca gcgttggtaa ttctgggaaa
    23641 agcagcaaaa ggatcatgcc aacatagatg acagcagtcg ctactttgcc ataccacttc
    23701 gctccactta atttgatatt tttagatagc ccgattaatc cagctacaaa cataaagcct
    23761 tcttttatca agaatagacc aaacaacagc caaagccacg gtcgatacca tgccatagac
    23821 aaaatcagaa aaagttgtgt caatttatct gcaaacggat caagcacctt ccctaagtct
    23881 gttacctgat tatatctccg agcaatgacc ccgtctagat aatcagacaa acctgagaca
    23941 acaatgaccg ctcctgctat ataaaatgac tgtctcagca caaaataaag ataaatcggc
    24001 agcaaaaaaa tacggaatat cgataaaaga ttcggaatcg tttgtatttc tttacgccag
    24061 tctgtgctta atttcatttt atcgccctac ctcctcagca ctcttttctt tttattttag
    24121 cttacacggg gaataaaacc aatgatttca ctgtttttat ttttctttac agacactgat
    24181 attatgagct aatttgagat aatcacttgc tgttttcatc gtttactctt ctttcttcct
    24241 gtcgtttgcg catttctttt actagtactt tatgagcttt ttttgatcct cgctctaggt
    24301 cacgctgccg tttacgtgtt ttataatcga taaacagact gtctaaatcg taacctgtcg
    24361 ggtacagatc ttctgctgaa cgcaccagac gaacacgctg acgaggcacg tggattattt
    24421 ttttgtctaa atatacatca atcgtatctt cacctatatc ttcataaact aacgcttctt
    24481 tttggtattc attgacaaat atccgatcac cttttgcaaa gagctgacgt tcttttttat
    24541 tctccttcct atttgtaact gctgtcttag gaagaaattc ttttttctgt gtccgatagg
    24601 ctttttccgc caagtaacgc tctgcttgat ggatcaattt catcgacata tccattttat
    24661 gagcaatcca caaagcttgg ctttctcctg tttcttccaa cccgcaactg atatttagga
    24721 cgaagtgctt cccgatcgaa agccatagca gccggcacaa aatcttcatg atctccggcg
    24781 aatttcttga tttctccata atgggtcgtg gcaatgatca atgccccttg ctcatacata
    24841 gattccatga ttgcgatcgc tagtgcagcc ccttcatttg gctctgttcc actgccaatc
    24901 tcatctaaaa gtaccaacgt attccttttt atctttttca aaatagcagc gatgttttgc
    24961 atatgaccag aaaatgtact caacgcattt tctaaatttt ggtggtcacc gacatcgacg
    25021 aaaatctcat caaagacagc aagctctgtc ccttcttttg ccggaacttg cataccgaac
    25081 atcgccatca aagtcaataa accgactgtc ttcaacacga ctgttttgcc tccggcattt
    25141 gctccggtga taatcaagcc tcgatagtct ttaccgattt caacatccaa tggtaccgca
    25201 tgctcaggta aaaatggatg tctgccttgc ttgataatga tatgttccga tttattgatt
    25261 ctaggtgtgc ttccattgat ttctcggcta aattttgccc gagcaaagat gatatctaac
    25321 accgtgatcg tttcgatcaa taaatcgatc acttcttcgt tttctgcgat agcaccagtc
    25381 aatgccgcca ggacttgata ttcttcactg atttcttcag cttttaacaa ctgatagtgt
    25441 tcattcaatt tagaaatcac agttggttca ataaaaaccg tcgttctttt atttgattca
    25501 tcgataatcg ttccatctat tttattttta tagctggctt tgataggaat cgtatagtat
    25561 tcgccttttt gaacaatcat tgcttcttgg atcatttctt tgtttttcgg atgacgcaag
    25621 aatttcagta attttgactg gatttctttt tcgattagtt gtaattgttt gcgtacttta
    25681 cgcaaattac gtgacgcgtc gtcactgact ttattatttt ttattttctg gtcaatcaac
    25741 tcttctacat ttatcaaatc aggaagatgt ttgctataag caaacaacaa gggtgtctgg
    25801 tattgattct tatcaaaaaa ctttgtgatc atccgactgc ttctaagaaa atccgcatat
    25861 tcgatcaaat cggttggctg cagaatcaac ccttttttga cttgttctgt cagtgcatcg
    25921 atcctcggca accccataaa tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct
    25981 cttgtttctt cttgacgatc caagacagtt tgtaggtttg tcgagaccgt catatcgctg
    26041 attcgttttt tgctatagtt tccaattgct cttgcttgga tttccttttt gatccgatca
    26101 aattgtaatt gactaatcat ttcgttgtcc atattctctc cttttcttga agagatacct
    26161 gtcccaaaat aaataagggc ctagagataa ataaaaggat agaacacagc ttaaaaaagt
    26221 catgtcctat ccttattaga atacaaagat attgttttcc tgacaggtac cgtttcataa
    26281 gcacaccaaa taaactttgt ttattggcta ttcgacttac tgattagata ccagctacac
    26341 tcacaaaaca taccccattc ttctaggtct tatttatttt ccacttaact atagcaagac
    26401 acattttggt tgtcaaactt tagtatgttt tatagattta caccattaat gaatagacac
    26461 ctaccgcgat aattgctccg atcattggac ctgcgattgg tacccatgaa tatgcccaat
    26521 ctgaagttcc tttattagct attggcaata ccgcatgtgc taatcgagga ccaaagtccc
    26581 gagcagggtt gattgcatat cctgttggcc ctcctaatga aagaccgatg gctaagatca
    26641 atccacctac agcgaatacg ttcgttccag gtgcaaattc gttttgtcca aaagctagca
    26701 acccaagaac taatacaaat gtaccaatta attctgtgat cacatttgct ggataattcc
    26761 gtatagcagg acctgttgca aacgttccta aaatcgcgct ttcatctttc gttgcattcc
    26821 aatgtggcaa ataagaaagc caaacaagaa tggcccctgc aaaagctcct aaaacttgcg
    26881 caatgatgaa aggaacgaca agattccaac taattgcccc tgtcatcgcc atagctagag
    26941 agactgcagg attcaaatga gctgggccca tgaatccaga aatatagaca gccattgtta
    27001 cagcgagtcc ccaaccaaat gcgatgacaa tccagccaga agcctgtgct ttacttttgt
    27061 ttaaattcac agccgcacac acaccatctc caagcaaaac taggatcagt gtcccaataa
    27121 actcccccat gatttgagtc atatcactgt tcatttattt ctcccccttt agttcccgca
    27181 gatctgattc tgcaatcaat gcttctaaac gtttagtctg ttgcgccttc tcctcttctg
    27241 tccattcaaa atatccagca attgcatcga tcacaggttg cttgatctca tcaagctgat
    27301 ctcgttcaaa caacagatga tttgtacgac gaatgagata atcgcctggg gccaaaacca
    27361 tttcttcttc taaaccataa cgtaaccgag ctgactcagc gagactcaaa ccaggataag
    27421 gtgccatttc ttttgctaat tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga
    27481 tataagtagc atcttcttcc tctaaaccgg ctgctactcc ttcttttgct aattctgtca
    27541 ctgtttcttc taatttagtt ggatcgaagt ttccacctga aatctgatat tttttagagt
    27601 cgatttcttt cgtctctatt ccgtattctt cttttaataa ttggcgtatc aatcgcaaag
    27661 ctccttctgc catcttacgg taatcggtga ttttcccacc agataaagtc agcaaaccat
    27721 ctggttctct ttctaaagaa ctacctctgg aaatcgtaga aggtgctttt tcatcacggc
    27781 tgttttccaa atggttcaac acgtcttcta cttcagcacg agaaactttg ttttccttat
    27841 attcacttac tgtatcgaca actttattga agctcttgtc tgaaatcgat ccattatctc
    27901 caccattgta atcagaacca gaattaccaa tcaacagtgg acgaagtcct gcccagcttg
    27961 cttcgatatc attcaatgtg atatttgctt ctggatagcg atgattgatc acatccaata
    28021 gataatctac atcttcttgt gtgactttcg gatccgtaaa atctccttgg taatccgtat
    28081 ctgtcgtacc aaagtaagtc ttgttttctc ttggaatagc aaaaaccatc cgcccatctt
    28141 gctttcctgt atcgaagtat gtgggttgcg gtacaggcag ttttttcgca tctacaacta
    28201 aatggatccc tttagttgga cgcattttag gagagactgg acgcgtaaaa ttcaagtttc
    28261 gtactttatc tacccaagga ccactcgtat tgatcactaa ttttgctttg atttcgatca
    28321 cttcatctgt cagcaggtca cgagctttga cgccaacgat ttgatcgcca tcatataaaa
    28381 agccaaccgc tttcatttta cttactagat aagccccatc ttcggcagcc tttttgatat
    28441 tatcaatcac taaacgggca tcattgttgc ggaaatctag atagacaccc gcgcctttta
    28501 gcccttcttt tttcaaaaac ggttctcttt ccaatacttc ttctggtgtc agcgtataat
    28561 tctcatattt ggttcctgtc acatttgcca gtttgtcata aaggtccatt gctactttga
    28621 ccgagaacat attgaaggtt gttgctcctt cgccctcata gattggcaaa agcattggat
    28681 ctggttttgg gatgtgtggg gcaatacttt gtacgactgc acgttcacca actgtgtcag
    28741 ctactacttc cacatcaaat gtcttcagat aacgaatacc gccatgaaca agtttggtcg
    28801 agcgagagga agtcccttct gcaaaatcct gcatttcgat caatcctgtt ttgattcctg
    28861 atgctgctga ctggactgcc acaccggcac cagtgattcc gccaccgatg atcaacagat
    28921 ccaattcttc ttgctgcatt ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat
    28981 ggatttcctc cttattcaaa tgcttgggta gcttttactg cttttttcca accgttgtaa
    29041 agtttttctc ggcgctcttc ctccatttga acttcaaata gcttgccagc ttcataaaat
    29101 tcgcggatct cgtctgtatc tttccaataa ccgacagcta atcctgctaa aaatgctgcc
    29161 cctagcgccg ttgtttccaa gttttctgct cgttggatcg gtacattcaa gatatctgtt
    29221 tggaattgca tcagatattc attgtttgct gcaccgccat ctactttcaa tacaggaatc
    29281 ttgattccag tgtcatcttg catcgtatcg atgatatctc tcacttgata agcaatcgac
    29341 tgtaaagtag ctttaatgat atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc
    29401 cctcgtgctg cctgatccca ataaggagca cctagcccaa cgaatgcagg aacaacataa
    29461 acctcatctt cgcttgtaga gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac
    29521 atttgaagtc catctctcag ccattgaatc gaagaaccag ccacaaaaat acttccttct
    29581 aaagcatagt aaacctttcc atttatgccg taaccaattg tcgtcaataa attatttttg
    29641 gataattgcg gtttttcacc ggtattcatg acaataaatg aacctgtacc ataagtattc
    29701 ttgaccatgc ctggttcgaa tgccatttgt ccgaataatg cggcttgctg gtctcctgcc
    29761 atccctgaaa ttggtgcttc gcttccataa aaatgataac cttgcgtata accataaact
    29821 tctgagttag aagtgacttt tggtaacatc actcgaggaa tatttagtaa atctaaaatc
    29881 tcctgatccc aatctagctg atggatattg aacaacatcg tccgactggc attcgagtaa
    29941 tcagtaacat gtgcttgtcc atcggtcagt ttccatacta accaagaatc gatcgttcca
    30001 aacatcaatt cgcctttttc agctcgttct tgcgcgcctt ctacatgatc aagaatccag
    30061 cgtatcttcg ttgctgagaa ataggcatcg atcactaacc ctgttttttc gtggaaaaat
    30121 tcttgatagc cttcattttt taagttatct gcaattccag ctgactgtct ggattgccaa
    30181 acaactgcat gatagattgg acgtcctgta tctttttccc atattactgt tgtttcacgc
    30241 tggttcgtta tgccaatccc agcaatctga tttggtttga tccctgattc gatgaacgct
    30301 ccggcaataa ctgactgtac agaattccag atttcatttg catcatgttc cacccaactt
    30361 tcttgcggga aatattgcgt gaactctttt tgagaactgc cgatgtgtct tcctttcttg
    30421 tcaaagataa ttgctcgtga gctagttgta ccttggtcaa tcgccatgat atacgttgat
    30481 tctgtcattg tgattccctc cattatatgc aaacgctttc tttaactact cttatcatac
    30541 tactgtttta aaaaaagaaa ctgtcaaaat tttattcttt ttttcttttt ttgacagttt
    30601 tttattatga agctattttt taatcaattc ttttcttttt tcagcgtctc tattcttctt
    30661 ttcaatcgaa taatgtcgta aggtgacgca aattctgaca gataatattc acactgtttt
    30721 ttgtttctct ggcaaaatgt gatcaccaaa tcatattctt taccttcttc ataacttcca
    30781 atctcaatac cgccgattcc cctcagttct ctgatcagat attgttgaaa agcaatacga
    30841 taaatcggca gactttctaa atcgattcca atcactactg gttttgcaat ataaaaatcg
    30901 atcatcaaaa gaacattggc atactggatc atcaggtaac taagctcact atcttcagct
    30961 cttttcctcg gcaattgctg caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag
    31021 gaatgcccga gcattttctg ctgctgttcc aaaagccgat cacgctcata gacttcgatc
    31081 tttccaacaa agaaaaacca ttcattgttg atctgggaaa tttggtagcc tactgccttt
    31141 tcttctttga ttttcaaacg acgaggacga taatgccata acatcgtttc ccgaatgtaa
    31201 gtatctaaaa cagcggttgg taactttttg ctccgtgtca aatcatactg ataaaatatt
    31261 tcttcgtcta ctatcgaaaa agaaacaaaa aagctgtaaa acatcattgt ttcgtataca
    31321 ttcagttctg ctgctgttcg actaagatgc atcacgatga ttggatcgat tgcttgataa
    31381 agccggtccg actgatagag taatttcttt tctttcaaag ttgcgtacgt acttttttca
    31441 ttcaatagtc tttttttcgt aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc
    31501 gtaaacgttg tttctaatac acggtccaat cctagaatga acggtctttt ttctggtgta
    31561 ttttgcaaaa aaagcggtcg ctgatcttcc gggatatatt ggaatagttc ataataaaaa
    31621 tatcgtatct gtagctcttc tccgtataat tgaccgtttt tgatttgtaa tccaaattcc
    31681 tctaagagct ggttcaattc tttgatcttt cgaaaaacag aagactcact gatattaaac
    31741 gctgtcgcta atcgaacgat ggatacttgc tgatgttcta ataaatagac gagcatctta
    31801 aacttcaagc tttcctgcaa taaaaacaat agaatttttt ccaaacttac atcttcgtcc
    31861 aacacaagca gcgcctgata ctcatttcgc tgtacttcta cttttttttc catcattcgg
    31921 ccaatcaatt caatatcttc caaatattga tcaaacgagg cttttgacag ttggacatgc
    31981 tctctcaaac cattgataga ggctgttccc ccttgcagaa caaggtttcg caaaatcagg
    32041 atctgtaact gctcttttct ttctaataat gcttcaatct tcataggcag tattcgccac
    32101 ctttgaccac accatatctg gcacacggta ttctggctgg aaaaatagct catgttcttc
    32161 taactgttca aacgtccatc cttcttctac tgcaattgtc agttggtcaa gaaggtggaa
    32221 catctctttt ttgctttgga gctgtgctcc aagaatcttt ttttcagaat caaatagaaa
    32281 tttgagttta actttgcttt gatttattgc tgtaagcttg tagtctacgt atcctgtttc
    32341 aattttttgg ggataaaaaa tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata
    32401 gccaaaccac tgattgccga ctggacgata ggttccctga tcctttgtct gtgagccact
    32461 aattgtcttg ctgacatttc gtgctgtacg gacagcgtta tttactagag aaacatacat
    32521 tttttcattt gttggacgaa aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt
    32581 ttgagacgtt tgcaagtagt catctaccca gatcgttcca tcatcattca atgttaattg
    32641 ttcttcccac atctgattgt cgggacgaga atgattagcc agcaaaacaa gatcatagtt
    32701 ctcctttctt ttttccgtct ctacaatagc ggtttcattt tcggtcaaag acacaacttg
    32761 ttcttctaaa aaaagtgaaa gcccttgatt tttcatttcc tttactaacg gttctgtcat
    32821 ttcaggatct agataacgaa aaagaattga ctttcggctt tcgtaaagat ggatttgttt
    32881 ttttcttgat gccaaccctt cagctatttc gattcctgct tgtcctgcac caataattgc
    32941 aattttcttt gcattcatga tcttttgctg aagagccgtt acgttttgta tctttttaac
    33001 tgggtgaata agcgttgatt ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc
    33061 tagaatcaaa cgatcaaaat ccaatttttt tcctcctgct aaaacgactt ttttttcttt
    33121 caatcctatt actgtctttt ctgtgtaaac atggattcga tagcggtcag tcagttcttc
    33181 tttcgtgatc caagaaagtt gtttttcact ggtaaccttt ccttttaata tcaaattgaa
    33241 cccgctggga atccatccta acttcgattg cttttcaatc aaatatattt cacttgttgg
    33301 catcaattgg cgaagataaa tagccgcatg aatccctgca aacgacccgc cgacaattac
    33361 aatgttcatc tgcattcctc tttcttataa ggtgttctac tgctaacttc tcaatcagaa
    33421 aacacagtat tcataactcg ctttcaacct tatgatacat agcgatgcag cagaatcaac
    33481 cctctccatc ataccgtgtt ctaggtctgc ttctcaataa tgtactagtg gacctaattg
    33541 aagagtatca tttctgaaga gatgtttcaa aaaatgtact cttataaaag aaaaaaactc
    33601 gccttttacg tacctctgtc caaataggaa acagaaaaga acgaaaagac gagttttagc
    33661 gtgtgtaatg taatagttag aatagacaca tcatttattt ttaattcttt aatgcttcaa
    33721 caactgcttc gcctaattga tgagcagatt gaggattttg ccctgtaaga agccgcccat
    33781 caatttgaac cacttctgaa aaagctgctc cagactgata gtttgctcct ttttctttca
    33841 aagcatcttc tgctaagaat ggtacttcat tcgttgtacc atttgcttcc tcttcttcat
    33901 tactgaaccc agtcactgta cgtccagcta aaatagattt tccagactca tctgtaactg
    33961 gcaacaatcc tacaacaccg tgacaaacag ctgagaccag cccttcattt cgatagattt
    34021 gtgaagcgac tttcccaaga tctattgctt ctggaaaatc ccacattgtt ccgtggccac
    34081 cagcataata aaccgctgca taatcatttg catccacatc atcaggacgt aaggtatttc
    34141 ccaaggcccg atttctgaaa tcttcatctt cataataggc ccaatcgatt tcatccatat
    34201 tcaaaacact ttgcggttct aaaggtacat atccgccttt aggactgaca aaatctgcat
    34261 cgatcccatt ttttactaat acatcataaa aatgagtcag ttcacttaac cacaaacctg
    34321 taggacgctc gatcttttcg tacttttcta catttgttaa tacgatcaat actttcattt
    34381 tcccatcctc tttctataaa aattgcgtta ttgataacgt gctttcaaga aattatataa
    34441 tgggcttggc aaataaagct ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc
    34501 ctcacggttt tccaataatt tctctgccca gtcaggatcg acgatcattt gctgtccaat
    34561 tgctgcaagt tctgcatatt tcaatacctg ctctacatct tcgcgattgc ggatcccacc
    34621 gacaccaact aaagggatct ttccttgaat cgtgttatgg accacttcaa gaatcgattt
    34681 atcgttgtat ttatcagaac gagctattcg atcatacata tttaatgaaa catgcagata
    34741 atctagtttt gactcacgta atcgttcaag aagccagatc gtgtcttcaa aacggatacc
    34801 tggtgtttca tattcttctg gtgagaaacg ataccctacg atgaatggtt ttttagcata
    34861 ttgttcaacc gctgagaaaa ccgttgttaa taattcttca ataaaacgat aacgattttc
    34921 caagctaccg ccccattgat cctctcgtct attcgaatgc ggagagaaaa attgttggat
    34981 caaatacgta tttgctccat gcaactcgat tccatcaaat cctgattcaa tcgctctacg
    35041 tgtggctttg ccaaatgctt ggattgtttc aacgatttct tcttcactca ttgctcgtgg
    35101 tgtttccgca tcaggacgtt ttgctgcaac tgcacttgca gatacaggct gttctccaga
    35161 taaagttgct cgacttgtca ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc
    35221 ttctccttgg attgctttcg ctagctcact taatcgagga atcatttcat catgagcgat
    35281 gctgagttca cctggccagc cttttccgat atcttggaca tttgcagcac cagtaataac
    35341 agcaccaaga cctttagaac gttgtttata ataagtgatc tcatcttcgg tgatcactcc
    35401 attgaaaaag ctgagttgtg tcgtcatagg cgacagcatc aatcggttct tcaattgaac
    35461 tcctgaaggt aaagtaattg attgatcaaa ttgtgacatt gtcattcctc ccataattcg
    35521 ctctgattaa cctatcgaga atcatctatc attaaaccac tcgattagtt gtacatgcaa
    35581 ctaatttgct taaaaaattt tatttttttt gtaacttatc tgttgcatca ttcattgtct
    35641 gggctaattc tttacttgct tcatttccca aaactgcttt gtatgactga tgaagttctt
    35701 cccagctacc atcgacttcc ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg
    35761 acaagcgacc ttcgctaact gttttgacgt atcctttata ttctaatttt tcaacaaaac
    35821 gagcaatcgt tgatggggcg gaatagagca tttgtgcaat atctttttgt ggtattccat
    35881 ctttttcttt taatatcaat aaaataaaac cataagttgg tgccagacct gtccttctaa
    35941 acgcttcatc agctaattta ttcaacatgc gatctagctt tttcacgcta aagaaaagac
    36001 attcatctaa cgctttttca attggctttt ccatttcatc ccctccattg gcgtacatat
    36061 catagtatga tttttatttt ttgtcaaaaa acacggaata atgtccgcct cctctgtcta
    36121 gatttgaaaa aaagaagtag ccatcttcaa agaatttcgg cttgttttcg acaaaactcc
    36181 ttctggaaca ccgtgaatca gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa
    36241 aattttgtga aaatggcttt ccacattttt gcataaattt ggcttaatac cgaaaaactg
    36301 gcttttgaac accgtttata tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag
    36361 aacagccaaa caaaaatagt tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc
    36421 agctgattgt acaccgttta ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa
    36481 ggagcaattt caaaaaacag cttctcatgt tttatgacta tggtgacttg tgatcgagga
    36541 gttggcagga gaatactgtt tattcgtaaa gaaggggctg tgacaagact tttgtcacag
    36601 tcccttattt ccttttatta taggatttcg ccatttgatt ggatgacttg tctgtaccaa
    36661 tcaaatgatt ttttctttaa ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca
    36721 tagatatatc cataacgttt tgacatttgt gaagtagaag cgctgacgat atcaatgcag
    36781 ccccacgacg tataaccaaa taaatctact ccatctttga tagcttcctt catctgctca
    36841 atatgctgct tcaaatagtc aatgcggtaa tcgtcttgaa cggttccatc ttcttctact
    36901 ttgtctatag ctccgagtcc gttttcagct atgaacagtg gtttttgata cctatcatat
    36961 aattgattca aagcaattct caaaccctta ggatcaatct gccagcccca ttcacttgat
    37021 ggaagatacg gatttttaat tcctccagtt attaagtttc cttggacccc ttcccaattt
    37081 tctggatgag cactgactgc actagacatg tagtagctaa aagaaacaaa atcaacaggg
    37141 taggctgcta agatttcttc atcacctact tctattttta agttgacacc tttttcttca
    37201 aacattcgtt gcgtatacgc tggataatat cctcttgctt gaacatcact aaagaacaaa
    37261 gaagttcttt gctgctcaat tgttttttga atatcatccg gatcacaaga atacggaaca
    37321 agaagctgat agctgatcat acaaccgact tgtgaacctt tgataatttc atgacaagct
    37381 tttgtagcta aagcacttgc tataaactga tggtgtgcac cttggtattt cgcttgttcc
    37441 aaatggggat ggttttcttc aattactcct acgctaacaa aagggtgatg ttttacacag
    37501 ttgatctcat tgaaagtcat ccaatatttt actttatttt tatagcgttt gaatactgtt
    37561 tcagcgaaac gaacgtaaaa ctgaataagc tttcgattag gccatccacc atattctgta
    37621 accaaatata acggcatttc ataatgggaa agagaaacga ctggctcaat cccatatttt
    37681 agacattcgt caaacacttg atcataaaaa aataatcccg cttcattggg tgtttcttca
    37741 tcaccatgag gaaaaattct tgtccatgcg atagacatcc gatagcattt aaaacccatt
    37801 tcagcaaata aacggatgtc ttctttaaag tgatgataga aatcatttcc gtgtctttta
    37861 ggataataat acttgctgtt gggatcctgc gccgctttta cttgctgatg cgtcatcata
    37921 tgccattgat ctgtccattt ttctttaggt aaatcgctgt cgaatgtata cacatcagac
    37981 acagataatc ctttttcatc tacatcccat gcaccttcac attgattggc cgctgtcgct
    38041 ccgccccata aaaatccttc tggaaatttt ttcatatcga tccccctaaa cttgctagtt
    38101 ttcttgtttg attatttttc ttatgactag taatgatatt tttgaatcta ctactatttg
    38161 taacaataac cattgtggtc ggatcatatc cctctttgat taattcttgc aaatctactt
    38221 ccatcaataa atcgccttta cgtacttttt ttccatcaga tactgcactt tgaaagtatt
    38281 tgccatttaa attcactgta tcaataccga tatggatcag tatctctgct ccttcttcac
    38341 tgactacccc aattgcatgt ttcgttggaa aaactgttga aacttccccg tttacagaac
    38401 tgtagatttt tccgtccgtt ggaatgatcg caaacccttc tcctaaagat ccactagcaa
    38461 atgttggatc gtttacttca gataaatcaa ttatttcacc tgctgcagga gtacttaaca
    38521 ctatatcctt tgaggattct tcttcgattt catccttgta gccgaaaaga aaagttagca
    38581 tacaggaact gatcatcgct aagaaataac tagcaactag ccaaacaaat ccttcaccaa
    38641 tataaatcgg gaaagacaaa atacttcttg tagcaactgc caaagcaccg gctcctgcgg
    38701 caccagctac tccaccaaat atagctccac atatacaagc aatgtaaaat ggtcgtttat
    38761 atttaagagt cacaccataa acagcaggtt cagtgattcc aaataatgct gtgataaatg
    38821 cagataaaga taaacttttt actttcttat ttttcgtttt caaaaatacc ccaagtactg
    38881 ctcctgcttg cgcagctacg gcagtcatac atgctggacc tagcgtatca cgaccgtaga
    38941 aagaaagatt gttggtcatt actggtacaa gtccccaatg taacccaaaa atcactaaca
    39001 cttgccaaaa tccgccgata acagcaccag ataaaacagg gctaaagtta taaacagcta
    39061 aatatccagc tgcaatcagc tgacttgcat aatctgcaat tggacctaca actaataaaa
    39121 taattgggac catcacgatg atacaaatga atggtgtcaa taaatttcga atactttcgt
    39181 gcaagctttt gcgaatctta ggctcaatat aagaaagaac ccatacagct aaaattgctg
    39241 gaataaccgt acttgtatac cttgctagaa tcacgggcat ccctagaaat cgcaaggtta
    39301 tgctatcact atatgcactg acaatcgtag gataaacgag agctgctgca ataaccatag
    39361 cgatgaaacg atctgtattg aacttttttg cagcagtata tgccaaaaat ataggcaaga
    39421 aatagaaaaa tgcatcagct gctgcattca aaatgatata agttcctgat tcagtagtta
    39481 ataaattcaa actcgtacat aaggcgagta ctcctttgat caaaccactt cctgccaaga
    39541 cgcctaaagt cggggcaaaa acagcggcga tcatgtctat aaaagagttt aaaatgcctt
    39601 ttttctcagt tcctctcttt ccactagatg catcatcttc tagtttcatt tgtttggata
    39661 atacgtcata cacttcattt actgcatttc ctactacgac ttgaaacatt cctccagctt
    39721 cgactacagt gatcacacct tctagttgct ctaccttctc cttatctgcc ttcttattat
    39781 cttttagggt aaagcgtaac cttgttgcac aatggtaaac attgctgaca ttttcttcgc
    39841 ctccgacaaa tcggacaata tctttcgcta attgtgaata gtccattttt acttccccct
    39901 tgttaattaa tgccgtagca atccttctta caagataagt atagttctta gaaaacggtt
    39961 tctccattca attagtaccc cttctaatag cgaacgaaac tgtacaatag tgtgtatggt
    40021 ttacttagcc gagcaaatca ctcactgctt gaaagaaaac aagtatatcg tttctcttat
    40081 agtcaaagga gacaaaaaaa acacgcttga tcttcctttg ctgatgtata agtggtatct
    40141 caagctgata ctctgtgatt tttgtttcta cttggctttt gataagacag cacaacaaag
    40201 tctcggcatt taacggaagc ataactaggt cagaactagg agagattagt tgctgaatgg
    40261 aatctatctt tttatggatt tttatttgat tttcttcttt gtagtaatga tcaagcagtt
    40321 ttgttttctt tttttcttca actatggtca gcttttcacg caaaatctga taaatctccc
    40381 aagattcaaa ctcatctaga acactaggta gataaatcaa atcatcccac tcaaaaataa
    40441 gctcttgctc agtagtcaaa attgcatcat attccgttat tttgtttgat tgatttttga
    40501 aaagatacac tggttctatc cggcattctt tgatccacat gttcatactt tgttgaatct
    40561 gtttttttag tgtattcaaa atactaattg gctggttact aacaatcagt atctcccctt
    40621 gttttcgaaa aggattcaac gcttcggcaa gatacaaaac aaaataagcg ctttcagcta
    40681 aattgggacg taattgaaaa tgtattggga agaaacgccg aattaaataa atttctaaag
    40741 gataacggga gatgatcttt tgtacaaaat gattcatggt attatgtccg gcatccatcc
    40801 tcgttttcat ttgtttgata tgggttagca aaaattctgt ttcatggaac aataacgaaa
    40861 caggaagatc taaaaaacga ataatcgttt gctcaaatgc tcttagattc ttctctattt
    40921 ctttgttttt ttcatcatta atcatcaaat aatttaactc taatagtcga ttttcaattg
    40981 cttgtttttc ttgattggaa aaagaatagg gtagttctga tgataacttt tttgtgaggg
    41041 tctctaccat cggaataaat aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc
    41101 gaagaatggt caaaacgaga taccgagaaa atcgtatgaa gtcctctcct gaaacaatat
    41161 aattatatga tataagaacc tcttttaatg tcttttggac agtcggcatg atattggaaa
    41221 aaattgtact gatttgttga ggaaaccttg cgtgttggat caccgtttct gttccaacaa
    41281 gagataagaa aattcttttt gaattttctt cagcaatgat ttttagtcct tttgtcgacg
    41341 aaacttccag ttctaattta ggattgcgct cgatccaacg caaaatcgtt tttatttgta
    41401 atgatacggc tgtttttgaa agaaagaaaa tgtctgctat cttttgcata ctgacatacc
    41461 ctttttgaaa caataagtac gtcacaatag ttgaagctcg gaaatccatg ctggaataga
    41521 ttttatgttt cgtcacctct tgtatgattt gctgtttagt ttcagattca attgattcta
    41581 aaaaataacc tgaacgttta tttgactgga taatgatttt cttctcgtag atagcaagca
    41641 tatttttgat ttcagtttgg atcgttttct tgttggtacc taaagcttca gctaattctt
    41701 cgcttgttat ccaatgattc ttatttctta atactacaag aatttggagt tgtcgtttat
    41761 ttaagtccat ttgcttacac ctcctctttg ttataaacat acaggaaaaa cctctcaaat
    41821 gacaatagtc acttgcagag gtttaaaaat catttgaaga aagatctata ttcaaggttt
    41881 ttaccattag actggataat ttgtttataa tattcaaaac tatctttttt caatctccta
    41941 aagcttccgc tcccatcatt ttgtctatcc acataaataa agccatatcg cttcctcatt
    42001 tcccctgttc ctgccgatac gatatcgatt ggtccccacc ataagtaacc aatgatttgg
    42061 caaccatctt ttattgcctc agcaatttcc tgaagatgta tttttagata ctcacaacgt
    42121 tttggatcgt ggattcgatt caattcatct ggtgcttcat ctagtccaat gccattttca
    42181 actatgaata atggtaattc ataacggtct gtcaattcat tcatgacata acgaaaccct
    42241 agaggatcaa ttggccaact ccaaggttcg ggagattttt ccttcaaaaa gggattatcg
    42301 aaaccatttg ctccgccagt atctacgcgc atttctgctt cttttttata gacagcgcta
    42361 cgataataac taaatgcaat ataatctgcc gtgttagctt gcaagcactg ttcgtctcca
    42421 tctaacatta ctggtgcaca gtcttcttcc ttccatgtcc tgtaaatata agctggataa
    42481 tgccctcggc acatgacatc ggagaaaaac catgaattgc gtttaaattg cagtgaacca
    42541 aatacattct ctggatcaca gtcatatgca taagtcgcta atgcagaaaa gctacacata
    42601 atacccatct tagcatttgg atcgattttc tttaataatt gagtagcacg ggcgttcgct
    42661 agaaacatat gatgacaggc ttgataaatc actttttttg ttagtgtagc aatgggttca
    42721 agtttatctc tagcagggaa tgtaaccaat ccagcagctg cataaggcat acgaaaagca
    42781 ttattgatct cattaaaggt catccaatat ttcactttcc cttggtagcg ttcaaagacg
    42841 gtctgcgcat aaaattcaaa aaagtcgatt aattttcgat tttgccaccc cccatactct
    42901 cctaccaatg ctaagggggt ttcataatga ctaagagtaa ttactggctc gatatcatat
    42961 cgatttaatg tattgattag tctgtcataa aatacgagac cagcctcatt tggcttctgt
    43021 tcgtctcctc ttggaaatat tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct
    43081 aactccttca gtaaacgaat atcctcttca aatcgatgat aaaaatcaat cgcttcatga
    43141 gaaagataat ggagtgattc gtctaaagca atctcccaaa tctttttttc ttcattccac
    43201 tgaatactag gatgctggtt catgatcccg ataagcgtat ctgtcacatt aggtaatttg
    43261 ccgtcttcca accatgcacc ttcacattga ttagcagcta ctgctccgcc ccataaaaaa
    43321 ttttctggaa atatttcctg atccataact atctctcctc gctatcaaca atttcttact
    43381 tgaagtatag ctcgattcat caaaacaaga tattctaact atttcggcag aagagaagaa
    43441 aaaaattgaa tgctactctt catttttaat atttatatat aacaaataca taagacgatc
    43501 cccgaaagaa aacactcgtt aaccatctaa gcaaacgatt tattttactt ccaacacaaa
    43561 tgcttcataa ggtcgtaaat caactttgcc gtctggtaac tgtttcagag gataatttga
    43621 cacaataacc cttaaagctt cacgtggaat agtgaacgtc tgcttttcac tggacatatt
    43681 acaaataacg agccattgct ggtcttcata tatgcgagaa taggcaaaga catgctcagg
    43741 cgtcttttcc actaattcat agtctcccca gacgatgatg tcatgttttc tacgcaaatc
    43801 gatcaatttc ttgtacatat aaaaaacaga atcttgatct gctaacgctt cttctgcatt
    43861 gatttcttta taatttggat tgactcgaag ccatggaatt ccagtagtaa acccagcatg
    43921 ttcacgggcg ttccattgca taggcgtcct agcattgtct cttcctttcg ccttgattga
    43981 ttccaagatt tcctcttttg taaatccaga agaaatcctt tcgttataca tattgattgt
    44041 ctcaatatct tttgcttccg cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc
    44101 tccttgatag atataagggg tgcctttcat catatggagc aagatagcaa acatcttggc
    44161 actttctttt cgatacacat cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg
    44221 attattccaa aataaactat tccatccttc atcacctaaa gatgtttgcc atttggataa
    44281 tacttttttc agttcagaga tactcaaggg tttcaaatcc catttgtctt tgccttcttg
    44341 ctgatctaat cctatgtgtt cgaattggaa gaccatggat aattcatttc ttttaggatt
    44401 ggaataaagt ttagcgattt cggtagttgc tccccatgtt tcgccaacag tcatcagatt
    44461 tttgtcacca aaagttgctt gattcatttc ctgaagatac tcatgcagtt taggtccatt
    44521 acttgtgatt tctttatccg gttctttgcc aatcatatcg atcacgtcca tacgaaagcc
    44581 cccaatacct ttatcgatcc agaaattcat catttgataa atctcttggc gaacctgttt
    44641 atttttccaa ttcaaatcag gttgacgctt gctatacaag tgcagaaaat attgccccgt
    44701 tttttcatcc aactgccaag cagaaccact gaatattgag cgcaatctat taggctcttt
    44761 tccattgacc ggatcacgcc aaatataata gtcccgataa ggattttctt tatttttacg
    44821 ggcctcaatg aaccacggat gttcatcaga agtatgattc accaccagat ccatgatgat
    44881 acggatattt cttttctttg cttcttcaat cagttcttcc atttcttcca tcgtaccgaa
    44941 ttctgccatg attgcttgat aattactgat atcataccca ttgtcatcat tcggagactc
    45001 gtatactgga gaaagccaaa ttgcatcaat acctaaatac gccagataat ccaacctgga
    45061 aataatcccc cgcaaatctc caataccatc ttgatttgaa tcttgaaaac ttcttggata
    45121 gatttgataa acaactgaat ggtgccacca tttttgttcc atgattttcc tcctataaat
    45181 ggatgtttct attttttgct taatcattta ttttagtctc tctcactaaa aattgtaacg
    45241 ctttcaaata gctattgcac cttgttatcg gtaacagaat aaaacaaaaa aacagctgag
    45301 gaaattccct caactgtttt ttgaaaggat tgatttatgc agggaagaat gctgatttca
    45361 caacttcttt ataaacggtg ttcactcagc tgtcccttgg tattagctca aaaaacggca
    45421 aaatataaga aactattttt tcgttttttt ctctaatact caggccagaa cgctgatttc
    45481 acaacctctt tatttcacat tcaatatcca catgaatccc acaaatacaa agaacagacc
    45541 atacatgatt ggagatactt cttttcctct tttagctgct aacatggtaa ttggatagaa
    45601 gataaaacct aatgcaatcc catctgaaat actataagtc aaaggcatgc cgatcaagat
    45661 caagaatgaa gggatcgcaa tctctaagtt tccccattcg atttgtcgca aagattgtgc
    45721 catcaatact cctaccataa tcaatgctgg tgcagttact tgtgaagtaa caactgatag
    45781 taatggagaa aagaacaagc ctaacaagaa gaaaatccct gtagtaatag ctgtgatacc
    45841 tgaacgtcct ccaacggcga taccagctga tgattctaca aatgcaccaa caggagaagt
    45901 accaaataag gaacccgcaa gcatagctgt tgaatcagaa gctaaagctt tccctacacg
    45961 aggcatcttg ttgtctttca tgaaacctgc ttggtttgct aatccaacta aagttcctgc
    46021 tgtatcaaag aaagtcacaa gcaagaatgt cagcactacg acccacattt gaagagaatt
    46081 gatgtcccct acatgtttta atgcaactaa aaatgttggt ttcaaacttg gtgctgcggc
    46141 aacaatttca gaaggcattg gaatcaaacc tgtgaccaga cctaaaatcg ttgtagctgc
    46201 catcccgatg aagatgccac ctggtacacg ccgaacaagc aagatagcag ttacgactaa
    46261 tccaaaaatc gtcaaccaag tagaaccaac gttgagtgat cctaatccta ctaatgtaga
    46321 ttcattcgaa acgatgatcc caccttcgct cagaccaagg aaagcgataa atagaccgat
    46381 accgccagaa atcgcatatt tcaaatctgc tgggatcgca tcaatgatca tttcccgcag
    46441 cttgaagatt gtgatcaaaa taaaaatcaa agaagcaacg aatacgccgg ctaatgctgt
    46501 ttcccatgga atccccattc ctaaacaaac agaataagca aaaaaagcat tgatcccaag
    46561 agcaggtgcc gttccaattg gataacgggc taataccccc attaaaacac agccgatcgc
    46621 actggctaat gcagtcgcag taaacactgc tccttcatcc attccagaag cacctaatac
    46681 agttggattt acaaataaga tataagccat tgagataaat gttgtaaatc ctgccagtac
    46741 ctcacgtttt accgtggtgt tcaatttttc tatttcgaag taggaaagta ttttttcctt
    46801 catcttccat ccccctaaat ccgaacatta taaataaagt ccgttaaaat ttcgatttat
    46861 taataaaaca acgtgtttta tcatatacta aaacaaaaca taagtaaaca aaaaattcgg
    46921 attttaagca aaatccgaac gttccatctg attaaatccc tttatctcaa agttcatgaa
    46981 cattcacttt ggattacaag aatttcttta ttgtgttaat aaagtttctt cattctagtc
    47041 tcaaaaatca atagtgtgat ttgattttac catttcgttt ctgttcttct ttgatgaatt
    47101 cttttgtatc gacaggattc agattattta gtccgccaaa acctgtatcg accatttttc
    47161 cccagccttt tgtcttcttt tttcctgatt tgagattctt tgctcgaagc ttctcactat
    47221 gttttgctcg ccgatacttc aaataacctt ctaatcttcc cattagatcc actgtttttc
    47281 ctccttttta ttcaatcaga cgactcagct tttcctataa caaaataatt gccttcaggg
    47341 tcagaaaaat tgaaagtcaa gatgccattg ttatctgtga tttctcctgg ttcttccatg
    47401 ttctcgtaga tagtttcaaa ttcatccgta aaaaatacta aagaaggcgg tggtcctaat
    47461 acctctggtg aaaacttctg cacaaatact tttgggaata cgcctaattc taattctttt
    47521 gagatgctga gtacaatact cttaaattct tctggcaatt cgattgtttc agaaatttct
    47581 gcaccaattt tttctacaaa gaatcggcaa atcatctcta cgtcattgac ataaagcatc
    47641 acacgtgttt tttgtatcat catttgaccc ttctttctat ttgattttct ttcctgtcta
    47701 taaactgatt ccattatatc acgaaagcgc aaacaccttg tgtttgatac tattcaagaa
    47761 aaataaagca atagtttcta ggattgcttt cctccacaaa ctttttttac aaaaaaaaaa
    47821 cggcaatgaa tgccgctctt cttatttcga ttcttttata attctgcttc tgattcatct
    47881 ttggttagtg tgtgtctaac actagttacg ccaaatgttt gatcgatttt ttccttcaac
    47941 gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat tgatggattc aatgaccagc
    48001 accgctgctt gtgtttcttc cgtcagcatc gttctttggg cttcacgcca gttgagacag
    48061 cgacagatcg ctccttgttc atcatagtag atgatttctt ccggaagtgc cggagcatcg
    48121 gaatctgcac ctaaaggcag gaatgattcg ccaccttggg cttttcctaa atgcagattt
    48181 cctgcgatct tttcgatatc ttctccgccg caaggtacgc caaaagacaa ggaaatacta
    48241 ttatagatat ctacaagcgg attgattggg aaaaattccc gttcttgatt tgcacgtttc
    48301 agtaaagctt caatagaaga acgtgcacct tttttagtct tgaatgtggt aaatgcctga
    48361 cgccactgag caatgacttc gttttggctg aatgtttctt ctgtaaggta atttcttgct
    48421 tcttttgctg cctcattcaa taattgatga tacgtttctt cactttcttt tgttacgtgg
    48481 ttatcgatac catttacgga taagatttcg atggttgctt caggaaatac ttcccagaaa
    48541 ttggtatcca ctattacttt tttcatctgt tcacacctct tgattagaat aattgcttga
    48601 tgaatagata gtctgtttgg ggatcatctc ccatataaaa aacatgttcg ctgtatttga
    48661 taaatccttt tttacgataa aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc
    48721 aaatggctgt tttttgcttt tcttttgctt tttcaatagc tgtatctagt aaaaatgtac
    48781 cgataccttg atgtttaaat ttttttcgta catagattcg ctcgatttct aaaaaatgat
    48841 cagccatttc ctcagattga ctcgttcctg tatttagttt caggtaaccc gcaggctctt
    48901 gatcgatact cactaggaaa aaatcggttt ctttatcttt tagctcttct gtaagttttt
    48961 ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta tcttttccaa
    49021 atgtatctgt aaatgtttcg atactaattt gttgtagtaa gtctacatct gaaggcgtca
    49081 tttttcgaat ttcgtattta ttcacggctt cttcctttca atagatcctt ttattgcctt
    49141 ttttgacaaa attccaatct tcgctaacat tcttgcatac tttatttaaa agatattcca
    49201 attgttttgc ttcttcgtca gaaagaccat ttaatgcgac tgcattggag tagttgtttt
    49261 ctcgaatgat gaaaggatag atttctgctc cttttttagt cggatagagt ttcttgattt
    49321 ttttattttc tttatcttcc aaacgttcga tcatcccatt ggattctaat ttcttgattg
    49381 ctcgggcagt cgttgtacga tctactttga tcatttctgc tagtttttct tggatgattc
    49441 caggattttc gcaaatacgt actaaataaa gatactgtcc tcttgttaaa tcgacttctt
    49501 tgaattcgat attgctaata gaatctagcg ctcgggcaat cattccgatg tctcgaagga
    49561 tttcagccat ttcatcccac ctttattttg tttgtaagac tagttcacct ctttttgttg
    49621 caaatgcaac tattttcaat aaaaaaatca agttcttttt taataactag taaagtatat
    49681 tcttttcttc ttttacgaaa aagctcaatc acaatagatc tggtttttgt tttctaaaat
    49741 aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt ctatactaaa
    49801 aaatcccatg ttggaagttt cattattctc aataaacttg gcctcttcac cgtggatcgc
    49861 acaagcaaaa acaagtttat aatactgaaa aagctgtgga atatcttttt gtttattcgt
    49921 atcaaaaact gctcgcaact gagtagtttc tactgttaat cctgtttctt catagacttc
    49981 tttacgcaca ttttcttcag gagacaatcc tatttcagca aatccaccag gaagtgacca
    50041 ttctttcgtc cgtttatctt cgattagtag gaccttgcca tcttttttga tataggcacg
    50101 aacatctact tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc
    50161 gaaagtttct tttcctattt cactgatcag ttccaatgaa atcgtccgga tctcctcaaa
    50221 acgctcttta tcaaatacat cttttccata atatagacca gcttgagaaa gtgcgagtag
    50281 tcttttgtat tgagccaact tcttgttcat tctcttccct tcttttttag caaatgtatg
    50341 actatgtaat caagtataga agactggttt aattacggca aataaatata atgaaatctg
    50401 caataaataa aagaatgtta aaaaactcga gaacaaaaga cattcgactt ctctggggct
    50461 gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata agccccacca aacgaaaaat
    50521 atgacgagct attcttgtct ggctgttctt attaattgag gcaggacagc tttttcacaa
    50581 cctccccact aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca atattcatca
    50641 aacatgagaa gctgtttttt tgaaattgct ccttgtgact cggagctgaa cgacaggctc
    50701 acgacctctc ggtaaacagc gttcaaaagc caatttttcg atattaagcc atttctttgt
    50761 tacttattgt cattgatttc tttttctaat ttatccaata gtggagtgta atccacttga
    50821 gtatagtctg ttacatcaaa ttcatatagt gttcctaact ggaatttgtc atattttcgg
    50881 tcggcgatga tctttttgaa tgcttctttc gtaatatgat tggtcccttt cgttcgatct
    50941 tccaatacgt caccatagtg ataatgatcc atgatatagc tcaaatgacg atctgattca
    51001 cgatcacgtt tgtaccgacg ttgccataac acttcaaagt ctgctgttag gcgaatcgtg
    51061 atcacctgat atccgtatct atctgctaat tcttggagtc gtgctttttg cttatcacta
    51121 aaaggatact cggacacaat cactttcttt cctgcttcca tatacaaatc aagtgctttg
    51181 taatagaaag gataaacttt cgtcctttct tgttccgccc gttcttccaa attgttaaac
    51241 ccgatggatt ctgcatagta ttccttgatt tcatcgagcg tgatggcata catatctgga
    51301 aatcgttccc gtagtttatt cataagatat gtttttcctg taccaggact accagccaga
    51361 agaatgaaat actttttcat ctcatttatt tcctaagtta attggacttt ccaagttcat
    51421 aaacagtcca atcttatgct tgacaatttc ttttactgct tcatttgctg gaatcacatt
    51481 atggcgaaga gacttgttga tttctgtttc actaaaggtt tctttagcta ctttcgtcca
    51541 gttgactaaa agctccgttc caacattgaa tttgcggatc ccgttattga tcaattcagg
    51601 ataatcttct tctttcacac ctgttcctcc atggataact agggggacat caactactgc
    51661 atcgatttct ttcagcaagg gaatgtttac ttctgttttt gatttgaatt gtccatgatt
    51721 cgtaccgata gcaatagcta atgcatccac gcctgtagct tctacaaatt tcactgcgtc
    51781 ttccggtctt gtatatacct tgtcattttc agcgacatgg attccttctt ctgtaccgcc
    51841 gattgttcca agttctcctt ctacggaaac accgtgcgca tgggcatatt ctactactgc
    51901 ttttgtcttc aggatatttt ctttgaaagg cagatgggac ccatcataca tgacagaagt
    51961 atatccgctg tcgattgctt ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa
    52021 gactgcatca accatttctt cttctgcaat agctgtcatt acattgacta acacatgcat
    52081 gccgatatat ttagcggtat cgacacttgt ttgaataatg atcggcgaac ccatttcttt
    52141 tgctgcgcct agcatagcag gaagcatttc taggttatgc gtattaaaag ctccaactgt
    52201 aaaattcaac tcttctgctg tttttgttac ttctttcaat gttgtataca tcttttattg
    52261 ccaccttatc tttttatttg tttcctttga tcgttacttg ttttgcaatt tgtcgcatct
    52321 catccatttt gcccatgtag taatcgatcc cttgatcatc accagcacga gcagcatctg
    52381 cacgcttctg gttatagagg ctcatcagtt ttttataccc atctccgatc gtctgtttat
    52441 tttccaaact cttttctaaa tattggatgg cttgatcgtt cacatacaat tcagaaaatt
    52501 taagtccaac ttgttcataa agtttcgcta atttatgctt atcttgggta gtggaaatct
    52561 cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc gatagggctt
    52621 tttcttcttt tgccagaact tttttcggtt ctttttcttt tttcttaaaa aatccaaaca
    52681 tcattttccc tactttcaat taaaatgctt tcaaaactgg gcttaagatc caggcatata
    52741 agagtgctgc ggctactgta tctacatcgg ttgctgtagc attgttgaat cccatcgaat
    52801 taaagatcgt tactagcaaa gcgggcaata atgtaataaa gaaaccatga gcaataccgc
    52861 caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa tatccctgct gtccctccgg
    52921 caaagaaatt ggttagcata ccaggtaaaa tcatcgctaa gccgaacatg ggcaaagtaa
    52981 acatggcaat aatcgtacca atcgttgtcg taatgaaacc taaaatgaca gcatttgggc
    53041 tataaggaaa gaaaactgga caatctaagg cagggatcgc atcaggaact aatttcatag
    53101 ctatccctcg aaatgccggc acgatttcac ctaacagtag acgtacacca gctagcaata
    53161 catacacacc cactacgaat tggatggctt gtaaaaaggc aaacatgata tagttttgat
    53221 ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg atatatagcg
    53281 ggaccatcac gaccatgaca gacaaataag tatcttgcag aaattcgaaa tgtgtcggca
    53341 acttgatatc ttcgatcgac tttttatttt cgcctttctc accaaaaagc ttagcaacac
    53401 cggcttcaaa taagtacccg atcgtacaaa aatgacctaa agcaatatca tttgatccag
    53461 tgattttacg aatgatcggt tgcgctactg ccggcatagc aatggcgaaa caagctccga
    53521 taaaaccacc gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag ccaaagactg
    53581 tcgtcatcgt tgccatccat aaaattgctt gaccagttaa aaaaatgtac ttccattttg
    53641 tgaaacgagc caaaataata ttgaatacaa aaatggctaa gaacgtcagt gcaatatctc
    53701 tacctaaacc caaatcattc attgcttgac cattgataga ttcaatagaa gggatgatcc
    53761 cttgcatatg aaatccttca gtaaagattt tcccgaaata agttaaactt ccaacgatga
    53821 tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt ctttaacgtg ccagagatga
    53881 cttgtcctgc tgatttcttc tgcagtagta gacccaacat agcaatcaat gcaatggtga
    53941 tcgatgcctg agtcaaaata ttttcgatga taaaattgat aacgcccatg tttttaccct
    54001 cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga ttttttcttc
    54061 aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag gatcaactgc
    54121 gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg gtttcatgga
    54181 aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt tgttcaaaac
    54241 atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc atacgaataa
    54301 aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct caactactga
    54361 aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat ccaacttctc
    54421 ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt aggagtctac
    54481 tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga aatcgattgg
    54541 ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg gtcgggcatg
    54601 ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca ctgcacgaat
    54661 catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc tagcagctag
    54721 ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc tgtccgtcaa
    54781 aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt ttgtttcgaa
    54841 taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt ctttcatgac
    54901 atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta accgctgtag
    54961 ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag ttaaaatcgg
    55021 atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga aaaccagatt
    55081 gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag taatctcgat
    55141 ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca tcccatggtt
    55201 acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac tggcgatcgt
    55261 cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac caattacttc
    55321 ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat ttgttcggat
    55381 gttttctttc aatggattca tgacttggat atgattcttg attcttacaa tcagtgcagc
    55441 catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag gtacgtgtgt
    55501 ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta cccactcgat
    55561 tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat aatttctttc
    55621 tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa atgcagaaaa
    55681 gctactgtct tttatttctg tatttttcca attaatggca gagataaatt catgtttcct
    55741 gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa tatcatcttc
    55801 ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca ccaaaggaaa
    55861 ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca taaagaagtc
    55921 ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag atcgttctga
    55981 acctttataa accagtcctt gctttccata actgacgatc tctatcccat aactgaccag
    56041 tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt gaaattcttc
    56101 ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa ctagatcaaa
    56161 aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag tttctaatcg
    56221 agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg tacggatctc
    56281 cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac ggatcgtccg
    56341 ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt gggctgtcat
    56401 gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct tctgatttca
    56461 agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga agtggaaaga
    56521 aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca acgaaaaaga
    56581 catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt gtgaaaaagc
    56641 tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat ttttcgtttg
    56701 gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga ggttgtgaca
    56761 gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc atattttaca
    56821 aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag aaaaaggagc
    56881 tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc cagcataatc
    56941 ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa ataccatatc
    57001 tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc ttttcgatcg
    57061 actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc gtggatctgt
    57121 cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac accgtctgct
    57181 ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata acagcttctg
    57241 gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat cttttggatt
    57301 tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa tatctccgct
    57361 ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac ccctcctact
    57421 gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc tgtacctgga
    57481 ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact gcccccatat
    57541 gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc tccaccatga
    57601 ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc cactaacaaa
    57661 atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta ttacaaaaaa
    57721 ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat aaactgtttt
    57781 tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat gtgtcggcag
    57841 tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct gcgcttcctc
    57901 caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga cgatttggta
    57961 aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt ttctgatcac
    58021 tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac atatgcattt
    58081 gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca agcctgatat
    58141 gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt tgcttcaccc
    58201 tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca gcaagcttgc
    58261 tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt ttttgtgcaa
    58321 tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg atgccatttg
    58381 tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt tctcttaact
    58441 cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa ggagttagcg
    58501 tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc gcatccagtg
    58561 catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct tcctctacca
    58621 acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg acactcactc
    58681 cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg atatgaagta
    58741 accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga atcatgactc
    58801 ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg aaaacaaata
    58861 cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat ggcaattttg
    58921 gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg atgactaaac
    58981 tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag tctcctggat
    59041 gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt aagaaaagat
    59101 aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg gctaccccat
    59161 aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga taaggcccag
    59221 tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt gaaccaatca
    59281 atactggcca tacatatttt ttattagcaa atactttgta accgagccaa acaaaaaacg
    59341 gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc gctccatcac
    59401 ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt tgttggactg
    59461 ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact agttcaaaaa
    59521 acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc atattataaa
    59581 caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag gtaccaagaa
    59641 aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc gtttgcatca
    59701 taacagccgt tacagctgat actttctgga agtcacttac gttgattttg atttttttgt
    59761 tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg ttaatttagg
    59821 tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact cttttagtga
    59881 aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag caggatgcga
    59941 ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca aatcaaaatc
    60001 actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc tgccgccgat
    60061 tccccattta catcctgata atatgtttct agcagcatga aattgctgat aaacaggata
    60121 atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg gatgaatgac
    60181 ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg caggtccacg
    60241 taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg ttccgaacat
    60301 cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat gtttatcaaa
    60361 aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt ccattttggc
    60421 acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg tgtatcgatt
    60481 tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga ttgcttcgat
    60541 gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta ggaaatgata
    60601 gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa ctttttcagc
    60661 agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt ctttagacat
    60721 cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt gtaatccatg
    60781 tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg gatgtccagc
    60841 ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga taccgatcaa
    60901 ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga ttggccgttt
    60961 cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca tgcggaaacc
    61021 ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag gatcaggtac
    61081 gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat acacagtact
    61141 aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt aaccaacact
    61201 tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt ttgcttgatg
    61261 gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat ctaaagaatc
    61321 aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc gttcgtccat
    61381 tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt catcaatgat
    61441 ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt tgtcactaga
    61501 atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca aaccgactct
    61561 cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca taccgccaaa
    61621 tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta gagcaatgat
    61681 tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca aacgaacacc
    61741 acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt cttctaacac
    61801 atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc caccttggaa
    61861 ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac ggctgacaac
    61921 gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg cgatgatttg
    61981 atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc gttctctggc
    62041 gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc gtcaatcgta
    62101 ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct gaaattggat
    62161 aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca tcgcttcctg
    62221 tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt tccaaatctc
    62281 ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt ggatatttat
    62341 aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca cgttctgctt
    62401 attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc gccaatttta
    62461 tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt cgatcaaatc
    62521 atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga tgcctagatc
    62581 ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt tcgtgctagt
    62641 cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt cttcttttgt
    62701 ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag tatcttcgct
    62761 ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta gtgcatctaa
    62821 ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt gaacaactaa
    62881 tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg ctggtgtaga
    62941 atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac cagataaacg
    63001 tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg aaccgataaa
    63061 catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc gcatcgtgcc
    63121 gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca taccaacttc
    63181 gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa tcatcgttgg
    63241 atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg attcaccata
    63301 agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat tcaaagattg
    63361 tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa tttgcaattc
    63421 tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt tatctgcttt
    63481 cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat cgacaacttc
    63541 gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga ttgttggtgt
    63601 ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat ctgcagctac
    63661 taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca aataagtatg
    63721 gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca agctttgtgc
    63781 accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat tcaaatgttc
    63841 tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg catctacttt
    63901 ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta atacaacttt
    63961 gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt ctacatactt
    64021 gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc gtttagaagc
    64081 ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata gaaacgtggc
    64141 gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta caccaaattt
    64201 tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca gatccattcc
    64261 taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta ggttttccaa
    64321 caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat tttttatata
    64381 caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt tcttgaccgt
    64441 atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg ctgtttgcgc
    64501 ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc cagataacgt
    64561 atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat caaatgtttt
    64621 tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca ttgcataacc
    64681 gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg ctgcattctc
    64741 aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat gtcctgctgc
    64801 aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg cttttgtcag
    64861 taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa ctaaccgata
    64921 ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta acatccctaa
    64981 cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg cctcctcagc
    65041 acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat gccaaaaaaa
    65101 gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg cttcatctgc
    65161 agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa gaattattta
    65221 tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta ggtgtccctt
    65281 tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa ttttatccat
    65341 atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct tcggtcaatg
    65401 agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct attccgaact
    65461 tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg caacatcatt
    65521 taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag cctggcatga
    65581 ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa catcctcttg
    65641 ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg atgctgtcaa
    65701 agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt atcgaattga
    65761 cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag caaggattag
    65821 ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct ttcgtcttgc
    65881 gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc ttcacagaag
    65941 atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta ggaaaataaa
    66001 aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa cggtgttcca
    66061 gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc aattttcgta
    66121 aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc tattgtccta
    66181 gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc atctagcttt
    66241 tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata ggctggaaaa
    66301 tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa atcaagaata
    66361 ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac agacatcagg
    66421 accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg aaatttttta
    66481 aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag ttcttttttc
    66541 gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat accacgtaag
    66601 aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt cttttttatc
    66661 aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat taaaatgttt
    66721 attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag ttcatgatga
    66781 aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa tcagttgaaa
    66841 cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga ttgatgtatt
    66901 cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct ttagtagaac
    66961 aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata gggacaaccg
    67021 aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa ggctgggaca
    67081 tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta acaaatgacc
    67141 tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat ctgcttttta
    67201 caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat gggacattga
    67261 aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag atcgatttga
    67321 cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt tatcatcgat
    67381 tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc catgaatccc
    67441 attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa acagaagaga
    67501 ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat tgccgattag
    67561 attcttctat ccataccaac agcacactct cctccctgta tgactctgca ttgaaaaaat
    67621 tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct tctttctgct
    67681 ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc tgcctgcatc
    67741 attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca gagctgtctc
    67801 ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata ttaaaatatc
    67861 atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt ttagaaacat
    67921 atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac gatctaaaaa
    67981 aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta ctgatccaac
    68041 aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc caaggagaac
    68101 cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa cgtgtctgct
    68161 tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca gatatcttga
    68221 tttccaattt ccctctagat tctctagata tccctattgt atacatttct tctattccaa
    68281 cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta tagtaacggc
    68341 tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt catgatccct
    68401 ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa taactgttgt
    68461 gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa aataaccatc
    68521 ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg caaaagacct
    68581 tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc ttgagctaag
    68641 acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg catagccaat
    68701 aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa atgataacca
    68761 gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg atctggcgac
    68821 tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca taaaatctgt
    68881 actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat caatccttga
    68941 gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa aatccgccgg
    69001 ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac catatcagct
    69061 gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc ggatcttttc
    69121 aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc atttttctgc
    69181 aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac atcgactatt
    69241 ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc ttctaaaact
    69301 ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc tttgtgatcc
    69361 cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc tttgactgca
    69421 ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg ggttaaagtc
    69481 tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa aacacgctga
    69541 ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc agaagaaaga
    69601 gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc tcctttttcc
    69661 tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt tgcttctatt
    69721 tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc tgtcggattg
    69781 ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca gtttttcata
    69841 atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat ttcatttttt
    69901 gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt ttctaccgaa
    69961 ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac ggatagaatc
    70021 atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc aatcgtagcc
    70081 gaaagaagaa tatgccaatc cataccatat cgaaatgtcg taaaaataac taagaatagg
    70141 ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt tttaatcaga
    70201 ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat ttctgtcgtg
    70261 tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt tcctgaaaaa
    70321 cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg acttttttca
    70381 gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg tgcttcgatt
    70441 aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc tccttttacc
    70501 agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc ttcttgtccc
    70561 atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca gtaactaacg
    70621 agtccattga cgatcactac gaataaaata atacttcctt caatatattc actagaagca
    70681 aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa atgctttgct
    70741 atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc ttgatcagtc
    70801 aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata gtttttcaat
    70861 acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta attgatactg
    70921 ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg tctgaaataa
    70981 ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt cttttatata
    71041 caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt gtcgtgcttt
    71101 cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga tctttatatc
    71161 tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac aagatcgaag
    71221 accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa aacaatcgac
    71281 tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct gtaattaaaa
    71341 taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc aaactttttt
    71401 cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt gctgaggaca
    71461 aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa tggatcactt
    71521 gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga acaacaaata
    71581 aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga cagttgattg
    71641 cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg atcatttcaa
    71701 ttccagcaaa actgaataaa accataggaa atgagactac cattccagca aagccaaacg
    71761 gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg gtatgaaaat
    71821 ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag atcttgatga
    71881 ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg actgccatta
    71941 agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac cagaaatgga
    72001 tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa agccaataag
    72061 tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa tcactgatag
    72121 agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa ccataaatag
    72181 ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca attgcctttg
    72241 ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac tggatatttc
    72301 gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt ttctttttca
    72361 agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat acagagtggt
    72421 actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca tgttccagct
    72481 ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac gacagtccaa
    72541 actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt caactttgca
    72601 tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat acagtagata
    72661 acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat cagcaaataa
    72721 aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt ggatacgtca
    72781 aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct agcactttct
    72841 gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac accaatgaag
    72901 acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg gcaataaatc
    72961 gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac atttgctaca
    73021 actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa tactccgcga
    73081 atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat ttcgatacca
    73141 gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt agtatctggt
    73201 attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc aactttgatc
    73261 cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc taaccaaaca
    73321 ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt gatgatccaa
    73381 taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg cacattatcg
    73441 ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc aggaaacatt
    73501 tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc attcgatacc
    73561 caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga gatcaatgag
    73621 attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt ccagctcgtt
    73681 gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat tccgataaag
    73741 gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc ttctttcaat
    73801 agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga caaggtttca
    73861 tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt agtgaactgg
    73921 gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa gaatatcttg
    73981 gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca aataattgtc
    74041 tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt ggatgatttc
    74101 gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga aaattcgatc
    74161 atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct gttctttttt
    74221 ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat caccgtaatc
    74281 ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat cgccatccat
    74341 ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca aatctgtaca
    74401 gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca atggatagac
    74461 ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc tgaatctttg
    74521 agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca atggcaatac
    74581 acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt ccaaaatata
    74641 agctcctaac atatctggag cctttactga tttttcaaac acatatggcg cgaaataaga
    74701 aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat aagggacata
    74761 gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc tgaacccttc
    74821 atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt caaagaattc
    74881 agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg aacgcgcata
    74941 gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt ttaacctttc
    75001 tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg ttcctacgac
    75061 agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg ttttttccaa
    75121 agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct gatctaagcc
    75181 gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag ggaccagcca
    75241 tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga cctcatccat
    75301 ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata aattcaaaag
    75361 ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag ctaaaggaat
    75421 cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac catctgctgc
    75481 aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc ctacttctgc
    75541 ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt tccacagcat
    75601 cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt gtccccagta
    75661 acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga cttcatccaa
    75721 gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat ctccttcgct
    75781 aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt catctaccat
    75841 cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta taaagacggc
    75901 cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa tgtcttgcag
    75961 atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga attttttttt
    76021 tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg gatttttctg
    76081 ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa aatagataac
    76141 ctaaaaaaag cgaacaaaca aa
//
LOCUS       DAZKOR010000002        61375 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000002 DAZKOR010000000
VERSION     DAZKOR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.002"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..562)
                     /locus_tag="ACG8ZM_000066"
     CDS             complement(<1..562)
                     /locus_tag="ACG8ZM_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFQ0491067.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(749..1399)
                     /locus_tag="ACG8ZM_000067"
     CDS             complement(749..1399)
                     /locus_tag="ACG8ZM_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491068.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1942..2877)
                     /gene="whiA"
                     /locus_tag="ACG8ZM_000068"
     CDS             complement(1942..2877)
                     /gene="whiA"
                     /locus_tag="ACG8ZM_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0491069.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2911..3909)
                     /gene="yvcK"
                     /locus_tag="ACG8ZM_000069"
     CDS             complement(2911..3909)
                     /gene="yvcK"
                     /locus_tag="ACG8ZM_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0491070.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3906..4790)
                     /gene="rapZ"
                     /locus_tag="ACG8ZM_000070"
     CDS             complement(3906..4790)
                     /gene="rapZ"
                     /locus_tag="ACG8ZM_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0491071.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4925..5656)
                     /locus_tag="ACG8ZM_000071"
     CDS             complement(4925..5656)
                     /locus_tag="ACG8ZM_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0491072.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5660..6925)
                     /locus_tag="ACG8ZM_000072"
     CDS             complement(5660..6925)
                     /locus_tag="ACG8ZM_000072"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0491073.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7188..10004)
                     /gene="uvrA"
                     /locus_tag="ACG8ZM_000073"
     CDS             complement(7188..10004)
                     /gene="uvrA"
                     /locus_tag="ACG8ZM_000073"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0491074.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10014..12008)
                     /gene="uvrB"
                     /locus_tag="ACG8ZM_000074"
     CDS             complement(10014..12008)
                     /gene="uvrB"
                     /locus_tag="ACG8ZM_000074"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0491075.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12267..13769
                     /locus_tag="ACG8ZM_000075"
     CDS             12267..13769
                     /locus_tag="ACG8ZM_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0491076.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13771..14508
                     /locus_tag="ACG8ZM_000076"
     CDS             13771..14508
                     /locus_tag="ACG8ZM_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491077.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14553..14726)
                     /locus_tag="ACG8ZM_000077"
     CDS             complement(14553..14726)
                     /locus_tag="ACG8ZM_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491078.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14738..15367
                     /locus_tag="ACG8ZM_000078"
     CDS             14738..15367
                     /locus_tag="ACG8ZM_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0491079.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(15449..16615)
                     /gene="dnaJ"
                     /locus_tag="ACG8ZM_000079"
     CDS             complement(15449..16615)
                     /gene="dnaJ"
                     /locus_tag="ACG8ZM_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0491080.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16766..18595)
                     /gene="dnaK"
                     /locus_tag="ACG8ZM_000080"
     CDS             complement(16766..18595)
                     /gene="dnaK"
                     /locus_tag="ACG8ZM_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0491081.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18646..19209)
                     /gene="grpE"
                     /locus_tag="ACG8ZM_000081"
     CDS             complement(18646..19209)
                     /gene="grpE"
                     /locus_tag="ACG8ZM_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0491082.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19303..20346)
                     /gene="hrcA"
                     /locus_tag="ACG8ZM_000082"
     CDS             complement(19303..20346)
                     /gene="hrcA"
                     /locus_tag="ACG8ZM_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0491083.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20580..21746)
                     /gene="hemW"
                     /locus_tag="ACG8ZM_000083"
     CDS             complement(20580..21746)
                     /gene="hemW"
                     /locus_tag="ACG8ZM_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFQ0491084.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21762..21989)
                     /locus_tag="ACG8ZM_000084"
     CDS             complement(21762..21989)
                     /locus_tag="ACG8ZM_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0491085.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22473..23080
                     /locus_tag="ACG8ZM_000085"
                     /pseudo
     CDS             22473..23080
                     /locus_tag="ACG8ZM_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            23253..23966
                     /locus_tag="ACG8ZM_000086"
     CDS             23253..23966
                     /locus_tag="ACG8ZM_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491086.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24081..25091)
                     /locus_tag="ACG8ZM_000087"
     CDS             complement(24081..25091)
                     /locus_tag="ACG8ZM_000087"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0491087.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25128..25610)
                     /locus_tag="ACG8ZM_000088"
     CDS             complement(25128..25610)
                     /locus_tag="ACG8ZM_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0491088.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25760..26128
                     /locus_tag="ACG8ZM_000089"
     CDS             25760..26128
                     /locus_tag="ACG8ZM_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0491089.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26593..27045
                     /locus_tag="ACG8ZM_000090"
     CDS             26593..27045
                     /locus_tag="ACG8ZM_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0491090.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27169..28020)
                     /locus_tag="ACG8ZM_000091"
     CDS             complement(27169..28020)
                     /locus_tag="ACG8ZM_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0491091.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28033..28818)
                     /locus_tag="ACG8ZM_000092"
     CDS             complement(28033..28818)
                     /locus_tag="ACG8ZM_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0491092.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29170..30111)
                     /gene="ribF"
                     /locus_tag="ACG8ZM_000093"
     CDS             complement(29170..30111)
                     /gene="ribF"
                     /locus_tag="ACG8ZM_000093"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0491093.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30115..31038)
                     /gene="truB"
                     /locus_tag="ACG8ZM_000094"
     CDS             complement(30115..31038)
                     /gene="truB"
                     /locus_tag="ACG8ZM_000094"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0491094.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31406..33768)
                     /locus_tag="ACG8ZM_000095"
                     /pseudo
     CDS             complement(31406..33768)
                     /locus_tag="ACG8ZM_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            33933..34389
                     /locus_tag="ACG8ZM_000096"
                     /pseudo
     CDS             33933..34389
                     /locus_tag="ACG8ZM_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(34546..34893)
                     /gene="rbfA"
                     /locus_tag="ACG8ZM_000097"
     CDS             complement(34546..34893)
                     /gene="rbfA"
                     /locus_tag="ACG8ZM_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0491095.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34920..37226)
                     /gene="infB"
                     /locus_tag="ACG8ZM_000098"
     CDS             complement(34920..37226)
                     /gene="infB"
                     /locus_tag="ACG8ZM_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0491096.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37239..37550)
                     /locus_tag="ACG8ZM_000099"
     CDS             complement(37239..37550)
                     /locus_tag="ACG8ZM_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0491097.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37547..37840)
                     /locus_tag="ACG8ZM_000100"
     CDS             complement(37547..37840)
                     /locus_tag="ACG8ZM_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0491098.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37862..39037)
                     /gene="nusA"
                     /locus_tag="ACG8ZM_000101"
     CDS             complement(37862..39037)
                     /gene="nusA"
                     /locus_tag="ACG8ZM_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0491099.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39060..39533)
                     /gene="rimP"
                     /locus_tag="ACG8ZM_000102"
     CDS             complement(39060..39533)
                     /gene="rimP"
                     /locus_tag="ACG8ZM_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0491100.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39678..44036)
                     /locus_tag="ACG8ZM_000103"
     CDS             complement(39678..44036)
                     /locus_tag="ACG8ZM_000103"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0491101.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44248..45957)
                     /locus_tag="ACG8ZM_000104"
     CDS             complement(44248..45957)
                     /locus_tag="ACG8ZM_000104"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0491102.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46025..47293)
                     /gene="rseP"
                     /locus_tag="ACG8ZM_000105"
     CDS             complement(46025..47293)
                     /gene="rseP"
                     /locus_tag="ACG8ZM_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0491103.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47454..48254)
                     /locus_tag="ACG8ZM_000106"
     CDS             complement(47454..48254)
                     /locus_tag="ACG8ZM_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0491104.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48251..49063)
                     /locus_tag="ACG8ZM_000107"
     CDS             complement(48251..49063)
                     /locus_tag="ACG8ZM_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0491105.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49258..49815)
                     /gene="frr"
                     /locus_tag="ACG8ZM_000108"
     CDS             complement(49258..49815)
                     /gene="frr"
                     /locus_tag="ACG8ZM_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0491106.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49818..50540)
                     /gene="pyrH"
                     /locus_tag="ACG8ZM_000109"
     CDS             complement(49818..50540)
                     /gene="pyrH"
                     /locus_tag="ACG8ZM_000109"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0491107.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50676..51557)
                     /gene="tsf"
                     /locus_tag="ACG8ZM_000110"
     CDS             complement(50676..51557)
                     /gene="tsf"
                     /locus_tag="ACG8ZM_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0491108.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51656..52438)
                     /gene="rpsB"
                     /locus_tag="ACG8ZM_000111"
     CDS             complement(51656..52438)
                     /gene="rpsB"
                     /locus_tag="ACG8ZM_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0491109.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52797..53276
                     /locus_tag="ACG8ZM_000112"
     CDS             52797..53276
                     /locus_tag="ACG8ZM_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0491110.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53493..54584
                     /gene="phnW"
                     /locus_tag="ACG8ZM_000113"
     CDS             53493..54584
                     /gene="phnW"
                     /locus_tag="ACG8ZM_000113"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0491111.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54577..54705
                     /locus_tag="ACG8ZM_000114"
     CDS             54577..54705
                     /locus_tag="ACG8ZM_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0491112.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54709..55254
                     /locus_tag="ACG8ZM_000115"
     CDS             54709..55254
                     /locus_tag="ACG8ZM_000115"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0491113.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55708..57399)
                     /gene="argS"
                     /locus_tag="ACG8ZM_000116"
     CDS             complement(55708..57399)
                     /gene="argS"
                     /locus_tag="ACG8ZM_000116"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0491114.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57819..58766)
                     /gene="arcC"
                     /locus_tag="ACG8ZM_000117"
     CDS             complement(57819..58766)
                     /gene="arcC"
                     /locus_tag="ACG8ZM_000117"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0491115.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58881..59900)
                     /gene="argF"
                     /locus_tag="ACG8ZM_000118"
     CDS             complement(58881..59900)
                     /gene="argF"
                     /locus_tag="ACG8ZM_000118"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0491116.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59991..61220)
                     /gene="arcA"
                     /locus_tag="ACG8ZM_000119"
     CDS             complement(59991..61220)
                     /gene="arcA"
                     /locus_tag="ACG8ZM_000119"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0491117.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT        17060 a        13104 c        10625 g        20586 t
ORIGIN      
        1 ctataaacat caccccaaat aaaacagaaa acagtataag cattataaaa attatcaaca
       61 accttgatcc ttcttttata gattcaggaa acagtataat tttagtacta gataaattgt
      121 ttacaccgat tagtgttaaa atatctttat tcttcttgct aacataaccc tttatgcaca
      181 aaagaacagc tatacttaaa gtaacaaata aataacgtat tccaatagaa aaatcactat
      241 aaatactctc tagcatagga ccgacaattg aagaagtcac tactgcaaat gctggaccta
      301 tccaagcaac attagaatgt tctttctctc ttttcctttg taaatctttg ttgagtatta
      361 atctatttat ttcttctgca ggcaagttaa ttttcataac cttatggggg attatccaat
      421 taaaaatcgg aaaaaggtag cctccccaaa aagtatagtg atctgtgtca atcagataat
      481 aacctgattt tgattccaga ataacatatc tcaaagccga acgaaaattt ttcatgaata
      541 tccataatct cactgtttcc acacctaatt cctccaattc caattaaatg gatttatagc
      601 atcaccaatt atgttaactg cctgaccaac ctcattgata aaattggcac cactatccca
      661 tgcttcaaca atgctatata tctatcaaac aaaaaatgga aaactaaata ctcgcgcaca
      721 taaattttac attctgtttt ttataagact aataataatc attgaaaaaa tttcttatcg
      781 gtggcgcata ttcccaattg attaatgtga tcccatattc tgcaagtatt ttacgagctt
      841 cttctaagtc atagtgagaa aaatgcataa aacctgcaat acgatcacgg actgtgagct
      901 gtggcttgct tccaccatca ttcataaaat atccagcaga gccactactt aaagtgattg
      961 ttacatcttc actttctatt attaagaatt cataatgaaa tgctttcaac tctccactat
     1021 tatttaaaag ttttatactc caacttcgtc taggttgagt tgtcacattc cctctaaaac
     1081 ttcctataaa ttctgcaatc accgtatctt caatcaaaat tctttcctca tctatttcat
     1141 attcaagact aaacggaaat tctccatatg taatctctgg caatggtggt gctgtagttc
     1201 tccaaataaa tacaataact gtgaatagaa aaaatccaac gatcgatata aatgttatga
     1261 gacctgctcg aagtaaaacc cgatataata aaaatgctgt atttcgcttt gctatatcta
     1321 gaaaaataaa aagtactgca atcataatga tagtaaaagt tgtaaaaaag cacatttttt
     1381 gccctccctt tataaacatc gattctcaaa ttctaaacca aatcttgata gttcaagttc
     1441 aggtataacg tataccaacc atacatccca attactattt atgaacttat gattatcact
     1501 gaataaatca ctccatcccc ttgttaaaac ttctacatta attacacctg gttgttttcg
     1561 actagctaat ccaatgaact tatcaaccat ccttatccac caaccacgag aatcttgacc
     1621 agaagtaaga aaaacagttc tcaaggttga tcgagcagtt ccttccagtt tttaattaat
     1681 ctccagttat agataaatac ttgatcctat ctatatcagg acttctatat taagcaaaag
     1741 tttatgtata aaactaaaat cttcattcag ttgtctcccc gctttttata cgattgtatt
     1801 aaaaaacaat agaagccttt caacattatg ttactccagt aaagttggaa ttatagtttg
     1861 acggaagaaa agcaagtata gcttttaaaa aaaactgata cattcgcatc tcctgcttca
     1921 tgtatcagtt ttttctttgg attaaactgc ttcttcacgc agtttttccg caaattcatt
     1981 tattttgcga attcggtgat taattcccga ttttgatatt gcacctgagg gaatcatttc
     2041 ccctaattct ttcaagctga cctctggatg ttgaagccga agttctgcga tttcctgaag
     2101 tttttcagga agggcatgta gaccgacctt tgcttcgatc aattcgatgt tttcgatttg
     2161 tttagaagcc gcatcaatcg tcttgttcag atttgctgtc tcacaattga ctagacgatt
     2221 gaccgaattt ctcatatccc gtacgattcg aacatcttca aatcggagca ttgagttggt
     2281 tgcaccaatc aatgttaaga aatcagcaat tttttctgct ccttttaaat acgaaatata
     2341 gccattgcgc cgttcaagcg ttcgagcatg tagaccgtaa taatttaaca tatcgcaaat
     2401 gtcttggtta tgctgttcat aaatagaaaa tatttccaaa tgataacgac tcgtttccgg
     2461 attattgact gaacctgagg ccataaaagc accacgtaag taagaacgca ttttttgcga
     2521 atttcccata atctcgtccg aaacgtgtcc attgaacatc ataccatcca tgatatcaag
     2581 atccatcaac acatcttttg tgccttgctt caaccggacg atatagacat tgttcttttt
     2641 taatttcatt tttttacgaa cgagtagctc acttcgtacc tgataatgat ctttcaaaag
     2701 agaatagata cgtctggcaa ttgcagcatt ttctgtttgg acgttcaaaa taaactgctg
     2761 gttcacaata cctaaagagc cgttcattcg cagcaaagca gctaattccg ctttcgcatg
     2821 ttctcgatga acttccaaac cagttagttc tttcttcaca tctgcagcaa atgacatcct
     2881 attctcctcc tttctccaac aataaattgc ttaatatttt gaaccgaaaa ctaaacggaa
     2941 caattcttct actactttgt ctccatcgtg gaagacacca ccatctttca attctaaaaa
     3001 gtcggtggaa atcactcgac atccttcatt tctcaatccg ttgaagtcgt gtgcgacttg
     3061 tactagatac tcatcataga tctctgggtc catataatct tcaggcactt tttccgtatt
     3121 gaccaatact gtatcgataa aaggctgatt caaatgcttg tgtaaaacac gtatatgatc
     3181 ggcatctgtg aaatgttccg tttccccttt ttgggtcatg atattacaga tatagacggt
     3241 ttcagcagtt gtcttgagca ttgcttcgcc gatttctggt atcactaaat ttggaaggat
     3301 actggtgaat aaacttccgg gtcccaaaac gaccatgtcg gcgtccataa ttgctgaaac
     3361 gacttttcga gccgctttgg cttcactttt atcatttgtg ttgcagacat acacacggtc
     3421 aatggtttta cggttgatcg cgattttaga ttctcctact gcagtcgttc cgtctttaaa
     3481 aacagcatgc aaggtcaaag gagtttctga agaaggataa acatgtccat ctacatgcat
     3541 catttttgct aataattgaa tcgcctcata agtgcttccc cgcatctcgg aaactgcggc
     3601 aatgatcaga ttaccgatcg tatgattggc taaaaactga tcttcctttt tgaaacggta
     3661 ttggaaaata tcttcataga attgtggcat atccgataac gccactaaga catttctcaa
     3721 gtcaccagga ggagccatag cgatcgattc tctgattgca ccgctgcttc caccatcatc
     3781 tgcaacagtc actactgctg tgatatctgc tccttgattt cgtaaacttt tcaggataac
     3841 tggcagacca gtgccaccgc cgaccactac gatctttggt tttcttatcc ggtaggtttt
     3901 cattttcacg accggttcac cgtctctttc cttttatcct tgtcccgatg tgtcacattg
     3961 actttatagt ttttccctaa agcatttgcc aaacgttcag tcaatgccac agaacggtgt
     4021 tgaccgcctg tacagccgat tgcaactgtc aagctgcttt ttccttcttt aatgtatcca
     4081 ggcaatacag tttccagaag atccaaaaat ttatggtaga atgattgcgt ttcatcaaag
     4141 gacatcacat aatcatagac tggctgatct tttccggtca atggtcttag ttccgggata
     4201 taatgagggt tgggcaaaaa acgcacatcc atcacaatat ccgaatcgat cggcaacccg
     4261 tatttaaaac caaatgatac aaattcgatc cgaaatcctt ggtctccctt tgatttgaat
     4321 gactcattga ttttttcacg taattgtcgt ggtgtcaatg ttgtcgtatc aatgatcacg
     4381 gaagcttggg ctttcaagtc gtctagtatc gcccgttctt tacgtatccc ttcagtgatc
     4441 aaaccgtcca tcgccattgg atgtgctcgt cttgtttctt tgtagcgagc cactagttct
     4501 tcgtctgaag catctaaaaa taagatagaa gtgtcgatga agttcgtatt ttcgatttcg
     4561 atcagcatgt tttggatttc ttcaaagaag gaacgagaac gcaaatccac tcctaaagcc
     4621 atttttgtga ctttacctga ttctttgatc aattcccaga attttggaat caatcgaggc
     4681 ggcatattat cgatacaaaa atagcccata tcttcaaaac tttggatagc tactgttttc
     4741 cctgcaccac tcatacctgt tatgatgact aattgcaaat tatctgtcat atttacgaac
     4801 ccctctcaca ttgtgcataa taaaattata tcatcccggg cgtatcatta ccagttttct
     4861 ttttctttta caaaaagatt tggaccaaat atcttttagg atatttgccc caaatccaat
     4921 ttttttactt ttccgatgct gtatccaatg cctcgcttct ttcagctaat gcacgttcga
     4981 tcgttcgatc cgctaaaata gattgagcat cgatcactgg gtaatgatta tcttcagcaa
     5041 attcatgcat caaagataat tctgtgcaac ctaggatgac tttttcacag tttaggcgct
     5101 ctaccgcttc ctctaagatt tcgtaataga gctcttgatt caaataatcc gattccttga
     5161 tctcatgata gatcaggtaa ttgatttttt cttgaagcgc agtatcagga atcactgttt
     5221 cgaaaccaag atttttgact tcgcgttcat agattccagc tttcatactt ccttctgttc
     5281 ctagaatcgc tactctccct gtcgtatgct gacggacaag ttcatttgct gcttctcttg
     5341 gcatatgcaa gataggaata tccgttgccg cttgtaactc ttcaaaaaag tagtgtgcgg
     5401 tgttacaagt caacacaatg aagtttggtc gcaataaatt ttgtttctca atatcatcca
     5461 gcagaaacgg cattggattt tcttctgatc gatctaaaat ataagctgta cgatcaggaa
     5521 ctgttgcatg attgaataag acataattca aatattcttg atcttttgta gctttcgttc
     5581 ggtggttgat caagcgaaca aaactttctg tcgccatcgt gcccattccg cctaaaatac
     5641 tgaaaaaatt ctccatgaac tagccttctt tcacatgaaa atactttttg aatcttggaa
     5701 tatagttatg gaacatatat ttcatcaaca accagcgctt gattgatcgg tctttttcat
     5761 aaaatacagt agtaccgtaa cgattttctt tgatcatttg ctcagctagt tttttgtctt
     5821 cattttcccg agcatattct aaaaagattt ttttgggcac acccatccat aatcttgctt
     5881 tatctgattg attcgttcca taggtcgttt caacaaaggg tttgttgaac actcgatctt
     5941 ctgtgacaaa acgagccaga ttcaagccgt tcaatgtcac aaagaaactg ctacgacctt
     6001 gtctcaaatt gatttcaaat aatttatatt ctccatctct aggatcatac ttcatatcga
     6061 aattggcaaa gcctgtatat tcgatttttt ccaaaaatgc cttaatggtt tgatagattt
     6121 tttcgttata atctggcatg atcacaacgt aattaccaat cgatgctggt gtaggatctt
     6181 ctaatagtgg atgaccaagg cacatcatcc gaacttgatg atcttcatct acatacgcat
     6241 ttagcacacg catattacta tcatcacccg ggataaaatc ttgtacaatc atttcacttt
     6301 tataaccggc ttcgtatatc cggcctaaaa ttaaatcgaa ctcttctcta gtctctaaaa
     6361 taaaagcttt ttttcttcct tcaaattgga cagaaagata ctccacactg tttgcaggtt
     6421 tcaaagcaac tgggaattca aaaggcaatt cctgttccaa atgtccattt actaacattt
     6481 cttctctgac gatcaatgtt ttaggatacg gaagatcata ttcttcacaa acttcataaa
     6541 agcttacttt attgatcagc ctttcaaata aagaataatc aatatatgga cagataaaat
     6601 attctgacag ctcttgttta tgttgggaga tcaattctgc atatccatca ccacaagcaa
     6661 tcaaaagata ttttttactc ttatcagaat atctttcttt tgccaaacga agcatcgtct
     6721 cgataaatac agggtcttta tcaaatcctg gaataacttc tacattgaca attttactgt
     6781 agcgggtagg ggctaactga tccgaagcat atgcttggca gattttgccg tatgcttcat
     6841 taaaagaacg tgccatacca taaacgttca tgtcgcttcc taataatata gggataaatt
     6901 cttcattttc aatactgttc atcattatct ctcacttact cataatctct tgtttttctt
     6961 ttccaactac acaaggaata tcataccata aagaaaaaag caatcaaagg aaagtcctcc
     7021 gattgctcaa ttcttttttg aacggattga acttatagga agtttttatc tataccaatc
     7081 agtgtagaaa gaactaccat gtaatgattc tagaacactg atattaataa aacaagcaaa
     7141 aattcatttt tttatgtttt tttaactatc tcagctatct atcctcttta taccattatt
     7201 tttttcaaat agtgacctgt ataactttct tttacgttaa tgatttcttc tggtgttcca
     7261 gtcgcaagga tcgttcctcc accttctcca ccttctggtc ctaaatcaat gatataatct
     7321 gctgttttga ttacatctag attgtgttca atcactaaaa ctgtgttacc agcatctact
     7381 aatctttgta atacatgcaa caagcgggcg atgtcatcgc tatgaagtcc tgtcgttggt
     7441 tcatctagta tatagaaatt ctttccatta gagattttgt gcaattcact ggcaagtttc
     7501 atccgttgtg cctcaccacc ggacaatgtc gttgctggtt gccccatcgt cacataacct
     7561 aagccaacat caacaatcgt ttgcagtttg cgatgaatct ttggaatgtg cttgaagaat
     7621 tctacagcat cctctactgt catttccaaa atatcagcaa tgctttttcc tttgtaatgc
     7681 acttctaatg tttcagaatt atatcgttta ccatgacata cttcacaagg aacatagaca
     7741 tcaggcaaaa agtgcatttc gatcttaata attccatccc cgcgacaagc ttcacaacga
     7801 ccgcctttta cgttgaaact aaaacgccct ttcttataac cccgcatttt tgcctcgttc
     7861 gtttgagcaa ataacccacg gatatcatca aatacacttg tataagtcgc tggattactt
     7921 ctcggcgtcc ggccgattgg gctttgatcg atatcgatga tcttttcgat actttcgtag
     7981 ccggaaattg tcttgaattt acctggctta gcagaattct tatttaattt ttgcgctaac
     8041 gatttcttta agatactatt gaccaatgta ctctttccag aaccagacac acctgtgact
     8101 gctacaaatt ctcctaatgg aaattcaaca ctaatatttt tcagattgtt ttctgaagca
     8161 ccagtgatct tgattgcttt cccattacct ttacggcgtt cttttggtac agggattgat
     8221 tttttccctg aaagatattt ccctgtcaaa gaattttcat cattagctac ttcttcagga
     8281 gtgcctgcag ccacgatttg tccgccttgt tctccggccc caggtccgac gtcaatcaaa
     8341 taatctgctg cccacatcgt atcttcatca tgttcgacta caacgagggt gttgccaaga
     8401 tcacgcattt tctttaatga gccaatcagt cgatcgttgt cgcgttgatg caatccgatc
     8461 gatggttcat ctaaaatata caaaactcca gaaagattcg atccaatttg tgtagccaat
     8521 ctgattcgct gcgcttctcc cccagataat gttccggaag cgcgactcaa agttagatag
     8581 tctagtccca cattttccaa gaatgaaagt ctgtcattta cttcttttaa aatcggtttg
     8641 gcaattactt tctcttgttc agataacgtt aagtttgaga agaaatctac tgctttttga
     8701 atagacaact catttgtttg cccgatattc gttccattga tttgaacaga tagagcttgg
     8761 ggattcaatc gataaccttt acatgtctga caagttaatt ctgtcatata aagacgcatt
     8821 tgctcacgcg tgaaatcact gttcgtatca tgataacgac gctcgatatt cttcaatacc
     8881 ccttcgaaag tcgtttctac atcgcgaacg ccaccaaaat cattttcata atggaaatgg
     8941 aaaagatcat ctgtcccatt caagatgatc tcctgatgct cttccggtaa ttcttcgaat
     9001 ggtgtatcta aatcgatacc gaaactttca caagcctgcg ctaacatttg aggataatac
     9061 tgagagctga ttggattcca tggaacgata gcaccttgac taagtgtctt cgttctatct
     9121 ggaatcacta aatcttgatc gacttctaat ttgacaccta aaccatcaca gtctggacaa
     9181 gctccaaaag gcgcattgaa tgagaacagt cttggttcta gttcgccaac agtaaatccg
     9241 caataaggac aggcatagtg ttcgctaaac aacatttctt cttcaccgat cacgtcaacc
     9301 agagcatagc cgtctgctaa tcgcagggcc gcctctaaag aatcaaatag gcgagaacga
     9361 atcccttctt tcacaatgat ccggtcgatg acgatcgcga tatcatgttt tttgtttttc
     9421 tccagctcag gcacttcact taaatcatag atctcaccat cgactcgaac gcggacgtat
     9481 ccctcttttt ggattcgttc gaatattttt ttatgttgtc cttttttctt taccacgatc
     9541 ggtgccaaca cttggatctt tgtgcgctca ggcaaagcca ttacttgatc tgccatttga
     9601 tctactgatt gactggtaat ctcgatatga tcgttgggac aaatcggatg tcctacacgt
     9661 gcaaacaaca agcgtaaata gtcattgatt tccgtcacag tacctactgt cgaccgagga
     9721 tttttacttg ttgttttctg atcaatagaa atcgccggac tcaatccgtc gatgctatct
     9781 acgtccggct tatccatttg tcctaaaaac tgacgagcat aagcagaaag gctctctacg
     9841 tatctccgct gcccttcagc gtacagcgta tcaaatgcta gggaactttt tccagaaccg
     9901 gacaaacctg taacgaccac gaatttatct cgtggaatcg ttacatctat atttttcaag
     9961 ttatgggcac gtgcaccatg aatcgtaatt ttatcgttag ccatgttgtc ctcctacttt
    10021 gaagctttta attctaaaat catatctcgc acattcgcgg ctgtttcaaa atccaaagct
    10081 tttgccgcat ctttcatttc tttttctaat ttcatcaata acgtgtcttt ttcttccttc
    10141 gtcatttctt cgtacgagac ttgtaccgct tcttttgtat catcttccga ttctttcgta
    10201 atagaaatca aatctcggat ttctttaatg atcgtttttg gaacgatccc atgttcttca
    10261 ttgtacttct gctgaatcgt acgacgtctt gacgtttcat ccatcgctag acgcatagaa
    10321 tctgttactt tgtctgcgta catgattact ttaccttctt cattccgagc agctcgtcca
    10381 atcgtttgga ctaaggaacg ttcactacgt aaaaagcctt ctttatccgc atccaagata
    10441 gcaacaaggg aaacttcagg tacgtctaat ccttcacgaa gcaaattgat tccaactaac
    10501 acatcaaatt cacctaatcg aagatctcga atgatttctg ttcgttccag cgttttgata
    10561 tcactatgca aatatttgac tttgatccct aattctttga aataatctgt gaggtcttct
    10621 gccatttttt tcgtcaatgt cgttacaaac acccgctggt ctttttcgac tcgttcattt
    10681 atttcgccta ctagatcatc gatttgtccc atgattggac gaacttcgat cactgggtca
    10741 agcagccctg tcggacggat gatttgttgg ataacggtat ctgtttgttc atgttcatat
    10801 ggtcctggcg tcgcagagac ataaatgatc tgattgacat gtttttcaaa ctcttctaac
    10861 cgaagtggcc gattgtctaa agcagatggc aaacggaaac cgtaatctac tagcatctgt
    10921 tttctcgctc ggtcaccatt atacattcct ctgatttgag gcatggttac gtgcgattcg
    10981 tccgccacga tcaaaaaatc ttcaggaaag aaatccaata gcgtataagg cggttcacct
    11041 tctttacgtc cgtccatatg ccgagaataa ttttcaattc ccgacgtgta gcccatttct
    11101 aacatcattt caatatcgta attggttcgc tgttctaaac gttgagcttc cagaagtttg
    11161 ttttcatttc gcaagacagt taaacgctgt tctaattccg ctttgatatt cgctacggca
    11221 tgctccatat gttcatcatt cgtcacaaag tgagtcgccg ggaagattgc tacgtgttcc
    11281 gtttctccta aaacttcacc tgtcagtgca ttgacttctc ggatacgttc gacctcatct
    11341 ccaaaaaact ccacacgtaa ggcacgttca tctcttgaag ccggaaaaat ctccacgaca
    11401 tcaccgcgca ctcgaaaacg accgcgctga aaatctatat catttctttc gaattggata
    11461 ctgactaagt cacgaatcaa ctgattgcga tctagttctg ctccctgtcg aatcgaaact
    11521 acttgttttt ggtactcaaa aggcgagcct aatccaaaaa tacatgaaac ggatgcaatg
    11581 acgatcacat catttctttc taataatgaa ctagtagccg aatgtcgtaa tttgtcgatc
    11641 tcatcattta cacttgaatc tttttcgata tacgtatcgc ttgaaggcac ataagcttca
    11701 ggctgataat aatcataata agaaacaaaa tattcaacag cattgttagg gaaaaattcc
    11761 ttgaattccc cgtacagctg accagccaat gttttgttat gggcaataat cagtgttggt
    11821 ttattcactt tctcaataag attggaaatc gtatatgttt tccctgttcc agtcgcacct
    11881 aataagattt gtgctttttt cccgccgact acgccatcca ctaattggtt aatcgcttca
    11941 ggctgatcac cagccggttg gtatttcgag acaagctcaa agtgtcggga ggtatctctc
    12001 tcaatcattc ttcgtcctcc ttttcattca ttccttttca ttttacgacc aataaaaaga
    12061 aaaggaaagc aaaggcattc cttactaaaa aattatacca caccgaacat actttcgcca
    12121 cttttttatc ttattataca tgttatgttt tataacataa aatgttagaa actcgcattt
    12181 ttttcatttt ttcgagctta cagtcttgtc tgattcagca cttttcgcct ataataataa
    12241 aatattggaa atttaggagg ttgattatga aaagaaagca tttatttcac tcatttgttt
    12301 tcttaatgac aggacttgtg acacttgcgt ttggtcatgt tgcacaagct gacaacacta
    12361 caacggcaga gtcaagcaca agcgagacaa tgcaaaaaat agagcccaaa aaagacgtat
    12421 acgtaattgc cagtgactct gcttttgctc catttgaatt tcaaaacgca aagggcgagt
    12481 acgaaggcat cgatgtaaaa ttgatggaaa acatcgccaa actgcaagga tttaacatcc
    12541 gaatggacta ccgtggtttc tctgctgctt tgcaagcttt ggaatcagga caagcagacg
    12601 gaatgattgc cggaatgact gtaacagatg aacgaaagaa aagctatgat ttttctgatt
    12661 cttactttga tagcgggatc caattagctg tcaaaaaagg agatacttcg atcaagtctt
    12721 atgaagattt gaaaggaaaa acagtcggag caaaagttgg taccgaaagt gctgaatttt
    12781 taagcgacca tgaatctgaa tatggctatt ctatcaaata ttttgatgct gctgatcaat
    12841 tgtacgacgg gttaaaagtt ggcgcaatcg atgcaatgat ggatgattat ccggtaatcg
    12901 gttatgcgat caaacaagga caagaattgg aaacaccgat caaaaaagaa tctggcggaa
    12961 aatatgcatt tgccgttaaa aaaggacaaa atcctgagct tcttaatatg ttcaatgaag
    13021 gattgaaaga attaaaaaga acaggcgaat acgataagat tattaaagaa tatgtagcgg
    13081 atggttcaga aacttccagt gagtcttcca ctgctgatga atcaacattt cttggactta
    13141 tccaaaacaa ctatcaagca ttgttgaaag gactttggag aacaattctc ctaactttga
    13201 tctcctttgc tttggctata gtaatcggtg tgatttttgg attattcagt gtagcaccaa
    13261 tacgcgctct tcgcacaatt gctagtattt atgtcgatat catccgagga attccaatga
    13321 tggttcttgc gttctttatt ttctttgggt tgcctggtat cgttggattt acgattcctg
    13381 acttccttgc tggtgttatc accttgactc ttaacgcaag tgcttatatc gcagagattg
    13441 ttcgaggcgg gatcaacgcc gttcctgtcg gacaaatgga agcttccaga agtttaggtc
    13501 tttcttataa tcggacaatg caaaaaatca ttttgccgca agcgatacgc atcatgatcc
    13561 catcattcgt caatcagttc gttatctcat taaaagacac aacgatcatt tccgcaatcg
    13621 gtgtagtcga attgctgcaa actggtaaga tcattgttgc acgtacaaca caaagtacgt
    13681 atgtttactt gatcattgcg atcatgtact tgattttgat cacagcattg acaaaattag
    13741 caaaagtttt agaaaagaag gtgaagtaag atggcagaaa aaatcttagt tgagcattta
    13801 gtcaaaaaat acggcgataa cacagtatta aacgacatca atgtatccat caacgaagga
    13861 gatgtcgtct gtatcattgg cccttcaggt tcaggaaaaa gtacattctt gcgttgcttg
    13921 aatcgtttag aagaaccttc tgacggtgaa attattattg acggcgccca cttgatggat
    13981 aaaagcacag atatcaactt ggtgagacaa catatcggaa tggtattcca acactttaac
    14041 ctattccctc atctttctat tttggaaaac atcattttag caccgatgga cctgaagaaa
    14101 gaatctagag aagaagcaga gaaaaaagca atcaaattac tgcagacagt tggtctagaa
    14161 gataaaaaag atatgtatcc tgaaatgctt tctggtggac aaaaacagcg tgtggctatt
    14221 gctcgtgctc tagcgatgaa ccctgacatc atgctttttg atgagcctac ttctgcactc
    14281 gatcctgaaa tggtcggtga tgtcttgaac gtcatgaaaa agttagcaga acaaggtatg
    14341 acgatggtta tcgttacgca cgaaatgggg ttcgctaaag aagtggccaa tcgcgtcatg
    14401 ttcattgacg acggaaactt tttagaagat ggaacgcctc aacaaatctt tgaaaatccg
    14461 caaaatgaac ggacaaaaga tttcttgaat aaagtattaa atatttaata aaaaccaaaa
    14521 atgcacggtc gccatgaagg gaccgtgtat ttttatcttc ttattttctc acctaatcgt
    14581 atcagccgca tcgcttgttt tcctgcacaa acaagcaatc gctctacctg ttccatcgca
    14641 taagaaagta ccatcggttc attgatgata tcaagtacat agtcgatacc accctcactt
    14701 aattgagctt gatttttctt ctccatatta tgtagttgtg ggacaacttt ctgacaatca
    14761 gcaattgtgt caagaacgga tcaatccttt attggagttg ctcgaaaggg tcttctctta
    14821 ttacggacag gctttgctca ctgtacatag acaacaaatc attatcctag tcaatcggat
    14881 ttccagagcc tcattgctat cattgcttga taaaatccag acaaagttta ataaaatgta
    14941 tcagttacag ctaaactttg gaattgggtc gctttgttat acagagcaag agacaccgca
    15001 atctttcctt catgccaaac aagtttgcga atggatagct ttccatcaat ctgtgaacga
    15061 gatccgcttt tttgaagatt tagacctcgg gatcgtttta ccagcaatcc cgtcagatca
    15121 acgaacatta tatgtaaaac gtattttgaa aagcctgaca gaagaagaag tacatttgtt
    15181 caagaagact ttggcatgct tcagtaaaaa taacggaagc atcaaaaatt gctcagaaga
    15241 attatttatt cataaaaaca ccttgcaata tcgattgaac aagttccatt ctctcactgg
    15301 ttatacccct agaaactatg acgattatca tattctaaag ctggcttttt tgttggtaca
    15361 aacatgacaa ctacaaaaaa accaggcgaa catatgattg ttcgtctggt ttttaattat
    15421 taatgactta gcgtttttac tagaaaaact acttccgctt tttcccgcca aaagcatctt
    15481 taaacttatc gaagaaaccg tcttcttgct gttcttcaac tttgatgccg ccagcttcag
    15541 cgaatgcacg taatgcttct ctttgttgtt catttagatt tttcggtgtg atcagtttta
    15601 ctttgacatg ttggtcgcct gttgcaccac cacgtaactt cggagcgcct tttccacgta
    15661 aacggaagtt cgtacctgtc tgtgttccag cagggatctt caatttcaca tctccatgga
    15721 cagttggtac ttggacttca tcccccaaag ctgcttgtac aaaacttaaa ggcaattcat
    15781 agtaaatttc agaaccttca cgatcaaaga tatcactttc ttctacacgg aagaccacat
    15841 acaaatcacc aaatggtccg ccgttttctc cagcttctcc ttgaccagct aaacgcattt
    15901 gttgtccttc ttctacacca gctggtacat tcactttaac tgaatgtgct ttcttctcat
    15961 gtcctgagcc atgacaggtt ggacaaggtt ctttgatttc tttccctgtc ccatgacaaa
    16021 catcacaagt ttgacggctc atcactcgtc caagtggtgt ttggcgttct acattaatcg
    16081 tacctgcacc atgacattta tgacaagtag ttggttgtgt gcctggtttt gcaccgttcc
    16141 caccacaagt atgacagatt tcttcccgat tatacttgat ttctttttct acaccaaaaa
    16201 tcgcttcttc aaaagacaag tcgaccgtat attgcaaatc agcaccttga cgaggcgcat
    16261 ttggatcgac tgaccgaccg cctcctccaa agaaagagtc gaagatatct tcaaaaccac
    16321 cgaagccgcc accagagaag ccgccgaatc caccaaatcc gccaccagct ccaccagcac
    16381 cgtaatttgg atctgtgcct gcatgcccgt attggtcata tgcagcgcgt ttttgcgggt
    16441 cacttaaaat ctcatatgct tctgatactt ctttgaattt ttcttccgca tcaggttctt
    16501 tattgatgtc aggatggtat tttttcgaaa gcttacgata agctttcttt atttcatctt
    16561 ctgatgctcc ttttgagagc cctaagactt cataataatc acgttttgtt gccataggct
    16621 ttcctcctat tggttctcat cattttcacg tcgcttgtta tcatatcata ttttgtttga
    16681 aacaaaaacc gatacttcaa actatttttc aaaagaaaaa gccaaagtag caactttggc
    16741 cttctctcga aattcagtga actgattatt tatcgtcgcc atcgacttct tcaaaatcag
    16801 catcaacgac atcatctgct ccgccttgag ctgcttctgg gttttcttga gcttgttgtt
    16861 gtgccgcttg ttcgtatagt ttaacagtca agttttgaac gatttcgttc aatgcatcgc
    16921 gtttagtttt catttcttcg atgttgtttg cttcaacagc ggctttcaat tcatcgcgag
    16981 ctgtttctgc ttttttcact tcttcttcgt ccactttgcc ttctaattct ttcaatgttt
    17041 tgtccacaga gaatagcaat gcatctacat cattacgtag gtcaacttcc tctttacgag
    17101 ctttatctgc ttctgcattt gcttctgcat cttttaccat acgttcgatt tcttcgtctg
    17161 ttagacctga agaagatttg atcgtgattt tttgttcttt ttgtgtacct agatctttcg
    17221 cagatacatt tacaatcccg tttttatcaa tatcgaatgt tacttcgatt tgtggaatac
    17281 cacgaggagc tgctggaata tctgtcaatt ggaatcttcc tagagttttg ttgtcagcag
    17341 ccattgggcg ttcaccttgt aatacatgga tgtctactgc tggttgatta tctgccgcag
    17401 ttgagaatac ttgtgattta cttgttggaa tcgttgtatt gcggtcaatc aatttagtga
    17461 acactccacc cattgtttcg atacctaatg acaatggtgt aacgtcaagt aaaacaacat
    17521 ctttcacatc accagtgatc acgccacctt ggatagcagc acccatagca actacttcat
    17581 cagggttaac tgatttgttt ggttcttttc ctgtttcttt acgtactgct tctacaactg
    17641 cagggatacg agtagatcca ccgactaaaa taacttcgtc gatttctgat tgtgataaac
    17701 ctgcatcttt tagcgcttga cgtactggta cttttgtacg ttctactaag tcagctgtta
    17761 attcatcaaa ttttgcacgt gtcaatgtca tttccaaatg caatggtcca gattctcctg
    17821 ctgtgataaa tggcaagctg atttgtgtgc ttgatacgcc agataaatct tttttcgctt
    17881 tttcagcagc gtctttcaag cgttgtaaag ccattttgtc ttgagacaag tcaatgctgt
    17941 tttctttttt gaattctgct accatgtagt cgatgatttt gttatcaaag tcgtccccac
    18001 ctagatggtt gtcgccagct gttgacaata catcaaagac accgtctcct agttcaagga
    18061 tagatacgtc aaaagtaccg ccaccaaggt cgaatactaa gattttttcg tcgcgatctg
    18121 ttttatctaa accataggca agagcagctg cagttggttc attgacgata cgttctactt
    18181 ctagaccagc gatcttacca gcatcttttg ttgcttgacg ttgtgcatca ttaaagtaag
    18241 ctggcacagt gataactgct ttttcgactt tttcacctaa atagtcttct gcgaatcctt
    18301 tgatatattg aaggatcatt gctgagattt cttgtggtgt atatgattta ccttctacat
    18361 ctactttgta acctgcttca ccgatatggc gtttgattga tgaaatcgta tttgggtttg
    18421 tcacagcttg acgttttgca acttccccta cttggatttc tccatttttg aatgatacaa
    18481 cagaaggtgt tgtacggttt ccttctgggt tagcgatgat ttttgcttcg tttccttcca
    18541 atactgctac tgcagagtta gtcgtaccta agtcaatacc gataatttta ctcataatta
    18601 acgtctcctt ctttaattga aagtgtaagt tttatatccg cacaattatt gtgcaacaat
    18661 gaccatggtt ggtcttaaca cgcgatcatg taatttgtag ccttcttgta gtacttctac
    18721 aatcgtatcg gctggtgtat cttcagtcgc tggaacagtt tgtactgctt gatgaagatt
    18781 aggatcaaag gcttctcctt tagcagggat tttttcaatt ccttcttctt ccagtgcgtt
    18841 acgcaaactt tccaagacca tttccacgcc ttttttcaaa cctgcccctt gatcatctga
    18901 cacttctgtg gctaatgcac gttccaggtt gtcaattgat ggaagtaatt ttttggctaa
    18961 atcttgtgaa cggtattttt gtaattgctc acgctcattt tttcctcgat ttgccatatt
    19021 tgcgatttca gcacgagccc gcaagtattt atcttccatt tcttctaact cagcttttaa
    19081 tttcgctgtt tcgaattctt ccgcttcagc ttctgaaata cccgaatctt ctgcagcttc
    19141 gacatcgatt tccggttctg gctgagcatc tgtcatttct ttatctagtt cttcttgatt
    19201 ttctttcacg atacagtctt cctttcatta ttcagtcagc tgtttgattg ctcttactaa
    19261 gaaagagaga acgatcagct gcaaatagct tcgttattca cattacgagt ttgacaagtc
    19321 aagcgaacgg tagtaatcag caagtttagc tgctagttct cttcgataaa catcgactag
    19381 accgaacatc cgagaataag gcatactagt aggccctaac aaggctattg tacctcttcc
    19441 atgaccatct atctcgtaac ttgcttgaat catactcata ttctgcaata aatcattgcc
    19501 taattcagca ccaatcttga tatgaatatt gctatccatc ggagtgatca tttgtgtaag
    19561 tccgtctgag tctttcataa aagaaaacat cgatttgaac tgatcaagat cttgacgggg
    19621 ctcataatcc aagagattca tttgtccact gacataaacc ttatcttcaa aaacatgacc
    19681 aagaacggtg tcaaataact gagacatccc ttctgtcgtt tggaaatatt tatgaagaat
    19741 catcggaatc tccgtacgaa gtttatggta caccgtcatc agaggatctc cgactagacg
    19801 gtcgtttaca atctgtacca ttttttctag ttcctgactt cccatatatt cgggaagagt
    19861 gaacacttgg ctttctacat tccccatatc tgttacgacg atagcaataa tctgtcgatt
    19921 attcaagggt acgatacgaa agccagtcag tcggcggtct tttatttcag gtcccaatga
    19981 aaaagtcgta tagcttgtca gctcagataa tatctcagca gattgcttaa taatctcatt
    20041 gatttcatga aattctctgc ctagagactg acgaatcagc agcaaatcat cttctgctac
    20101 gcgagcaggt tttaacaaat gatccacgta ataacgatag cctaaagcag atggaatccg
    20161 cccagaagaa gagtgagtct tcaacagcaa tccttcatct tctaatgctt tcatatcatt
    20221 gcgaatcgtt gctgagctgg ccttgactcc ctcagacatc aatgttttgg aaccaacggg
    20281 cacgccacta ctcgtatagt gttggatgat cagacgtaaa atgtcgtttt gtcgctttgt
    20341 taacatcttc ctcacctctc attagcactc atatattcta agtgctaatc cacttataaa
    20401 tatagcaaga agcccttctc ctgtcaagag aaagactcca ttttttagca ctctaaacac
    20461 aagagtgcta attctaaata attgatgtaa atccagttat atcaacgttt caatagtcct
    20521 ttattgtatt tttgaacttt ttttatttaa attcagataa aaaacaaata ttctttaaac
    20581 tatttttcaa acaaaaagcg ctcaaacaca ttgttcccca aaaaggtccc tcttggtgtc
    20641 aaataaacac ggtcttcttc attaacaagt aacccttctt gctctaattc ccttagcaca
    20701 tcaccgtaca ccgattcaat tgttttttga tagcgatcat agaaatgttt tttagtaatc
    20761 cctgtttttt ttcttagtcc taagaacatt tcttcttcta tttgattttt tcgtgtcaat
    20821 ttttcttctt ccaataccgg gcgttcattt gctcgtaatg ggcgtaaata gtgttggatt
    20881 ggtcctttgt tgcggtaacg aatatctcca agataaccgc tagcaccagc tccaaaccca
    20941 aaatagtggg cattgttcca gtacatgagg ttatgttgag attcatgtcc ggagagccca
    21001 aagttactta tttcatattg gtgacgtcca tgcttttcca ttgcttcaat cgtctcttca
    21061 aacattctgg tttccgtttc ctgatcaggc agttcgagtc ttccttgtcg tacccagttc
    21121 atgaacattg ttttgttttc gaggatcaat gagtacaaag aatagtgagg cagatccaaa
    21181 gccaaagctt tttccaaagt atccagatat ccttctagtg tttgtcccgg taatgcataa
    21241 atcaaatcga tgcttacatt ggtaaaattt tctgcttcta aaaattgcat agtttgatac
    21301 acatcttctg cggtgtgttt acgtccaatt tttttcaata agcgattatc aaatgtctgc
    21361 actcccatgg ataaacgatt gacgccatag tttttcatta cttgaagttt ttcacgggtc
    21421 aaatcacctg gattcgcttc tacagtaaac tcattaccat tttgaaaagg caggatctct
    21481 ctagcacctg ataacagtcg atcaagttgt ttagcagata aagaagttgg tgtcccgccc
    21541 cctacgtaaa gtgtttctaa attcgctgtc ggatgacttt ctagcattat tttcatttca
    21601 ttcagcagca tttccacata ctcatctact ggctgccctt ctaaaaaaac tttattaaaa
    21661 tcacagtaat agcagatatg ctcgcaaaat gggatatgaa tgtacgcaga aaccttgttt
    21721 tctgcgggat ttattgtttc tatcattgaa attctttctc cttacgtcga aaagtcgtgc
    21781 ctgttttcga ccttcttaaa atcgcgttca agcaccaact cattgaaata agccgcatat
    21841 ttagaatcaa ggaaacctag ctctaaaaat gcttgcttat tgtcatcgga gctacacggt
    21901 gcttttacag cctctcgata aacggtaatc gcaaagctga ccttcggcat taagtcaaaa
    21961 tctgcaaaaa tatgaaaagc tattttcaca gattcctcct taagtgctca gaccacaacg
    22021 ctttgcttac aacctctcat atacgcatac taaacgtatt tggcgaacaa atccactaaa
    22081 aaatccaaga atcgacttct tggatgattt tttctttcat taaactggtt acctagttat
    22141 atatctctag ggttgttttc atgtctgagt gcccgatatg atttattttt cgtttctata
    22201 attacttttt gccttccagt ttcttttaat attgtactca agcaagtggg aattggttct
    22261 tttgtcatcc atgtatgcaa aatgttataa tgctggtaaa caaaaatgat tacctgtcat
    22321 ttttatttag ttcgtattta attaagttga ctatctgtat actctgtcta aaacttaaga
    22381 attgttgttt tcgctgtcgc tacccaattt taatgaatgt ctgtaggtta aaactgttgt
    22441 ccttgttata gatagtaaag gagagcttga aaatgaaaac tgcgattacg tttctattag
    22501 aggaatatgc cgattgggaa ggtgcttata ttactagcac actaaaccaa aatgagaaat
    22561 ggcaaaacaa aactgcttct atccaatcag aggtcacttc cattggagga ttaaaaacaa
    22621 aaatcgatta cttaatcgaa caaatacctg aaaaagttga cctttttatt atgatcggtg
    22681 gcaattcatg ggacataaaa aatcatcatt tatatcaaat aatcgataaa ttgttaaaaa
    22741 cagacgtacc agtagctgct atttgtgggg cagttgatta tttagctcag agcggattat
    22801 tgcaaggtta caaacacacc ggaaatgcgc aatttccttg gttagaatcg gaaaattatc
    22861 agaataaaga agattttata gaacaacaag ctgtgaaaga tcgtaatttg attactgcga
    22921 atggaacagc tcctatagaa ttcacagact tgattcttca agcagtccat tttgaaacag
    22981 aagaaataag agaaaaaacg attcgtctat acaaacttgg attctatgat tactataaaa
    23041 agtacggaga tcctttctcc taatcattct cttcaattaa atactcctta tcaatagtgt
    23101 gacaagtaat tttactgatg attatttgtc acactttttc tgacatttat caaagaagat
    23161 acactctatt tatattcata gacaaggttt tacggattca aaaattcagg attatgctat
    23221 actggtatta aaaaaataat gaggtatttt atatgaaaaa gttaggtttt attttattgt
    23281 tggtctcttt attccttgta ggatgtacgt ctcctagtaa agaaaatagt gatccatctg
    23341 cgaccacaga caaaagttca atcagacaaa ccagttcagc tgaaaaatca agtactcaga
    23401 aatcagcttc tactgaaacg aatacatctt caacaagcag taaagtctca gagcccaata
    23461 cttcttctac atctagtagt acagttgaga caaatatttc ttcttcaaca atagacacaa
    23521 cctcctcaca gtcttctcag tcaatagatc cgttagcagg atattctgat ctacaaattg
    23581 agtacgcacg tgtttggcta gctattagag gtaatacttt tagagataca tcgggtgatt
    23641 tcgaattaca tgttattcat tatcctgcag gaacacctat cgccccttat gatgcaggga
    23701 gtgcagtcta tccgaaagat gtggttatgc tcactgggaa atacagttac cagggagttt
    23761 tagtgtattc aagtaaccat gacgggacaa tcactagtta tccagttcct tcacattggc
    23821 aaatgcctgc agatcaaaca tcttatccag cgttcattag agaaacaact caagaaatca
    23881 ttgataatgc atctgtagta gctattccta caggaaatcc caatgacgtg aaacagttaa
    23941 tcgatgtgac agttatagat aattagactt tctaatatgt tatacaaaaa gaagctgaca
    24001 gcattttgtc agcttctttc actttctttt ctatttagat ttattggtct ttgatagact
    24061 ccaaggcagg aaaaaatttt ttaaataaac gtttgcaaaa cagtcgcttt tctatcttct
    24121 accgctactt ctgcataggc accattaatc aatgtcagtt gtggcggtac atgaccacaa
    24181 tcaatatcat acaagatcgg tagttttaat tcctttgaca gatcttcgta tacatccttc
    24241 attttataat ctaacaaatc ctctgccgca cttctaccga acatgatccc cgagctgcca
    24301 tcaaaccagc ctgccagttt catctgaaca agtgatcttc ttaaatcaac tgacgatagc
    24361 tcacagtttt ctagaaacca caagatcggc tttccatcat tatactttct tttaaaagat
    24421 tggacatctc cataaggagt accgattaaa tgcctgatcg tatcaataca accacctaat
    24481 aatcgtccct tcatccttac tgcatctccg ctgactgatt gccaatttgt tggttcagtg
    24541 agatggaaaa cagaagatga tgaatgctca tggggccaca ctttttgata cttttcagaa
    24601 gattgctgta gaatcgattc tccttctctt gttttcagta cactttccca cattgctgtc
    24661 gtttcatcca agtactctcc tcttaaatca ataaggttag ttccatgtgc agtggctatc
    24721 cctgttttta atgtgacagc aagtagcaac gtactaatat ctgaataacc tactatccat
    24781 tttggttgta gagcatcaaa atcgacaaaa tcgagaatct caatcaataa ttctcctccc
    24841 caagtcggaa agatcatttg gatattttca tcttgcatca tttgattgaa ttcatctgct
    24901 cgttttcttg ctggacctga tttttcctta tcttgcgtcc aaagcgtctt tccagtaact
    24961 acttggaaag atttttcttc cattctcttt aaagctgatt cgagtaacga atggtgttcc
    25021 tctgatacgc ctgaggatgg tgcagtaacc ccaatcgttg catttctttc taataatgga
    25081 taacttatca tgttgcttca cctccaagta tgcactattt tacttggtta attgtttttt
    25141 tcgtttgccg gcatcaaaaa tatcggcatc tcaagtagct ggatgatttt gtttaagtca
    25201 accgtttgga tgaccatcgt ttgatctggt ctacctggtg caaaaacaag tttttcattt
    25261 gataagagct cttcatcaat aataatcgga atctgttttt cataaccaaa cggagaaaca
    25321 gcgccctgct tttgtcccgt ttgttcttcc atgcgttctg atgaaacaat agaaagcctt
    25381 ttaccgacga tcttccctaa tgctttgaaa tccgtttgtt tcgtagtaaa agtcagatat
    25441 acgtagttat tatctttttt atcttttaag aataagcttt tggtctctgt acctgtaaat
    25501 ccttgttctt tctttattac caaagcatct tcatttgtat aaatggctcg atgatgatac
    25561 aactcaaaat atatattttc gtgtaagaat ttctttgcca agtcaatcat ttttatgtct
    25621 cttttctatt ttattatttg agacatctta tcatataaat atagcggtta caacattact
    25681 tgtgttttgt tcgtttttta tacataacta ttgtttttct ggtaaaataa atcaaaccac
    25741 ttaatggagg cgttttagaa tggaaaaatt tcagccgatc atcaatcaaa tcaaagaaaa
    25801 agaacatcaa caaaagatca aagacctttt tgctaagata acagaaacgt atccccagct
    25861 agacacaact gtaaaatgga accaacctat gtttactgac catggtacct ttattattgc
    25921 atttagtttt gctaaaaatc atttttctat cgcaccggaa aaagcagcga ttcgtgccct
    25981 tgaaaaaaat atccaagaag caggctacgt atatacggat aacgtcatca aagttccttg
    26041 gaaaagtgta atcaactggg aattgattga acaattgatt tcatttaata ttgaagataa
    26101 aaaaggtcat gataaatttt ggcgataaag ctttatacga ataaaccttt tactggcgag
    26161 gaaactattc atcaccttat acaggttttg ttcaatccta tcctttttat catccctggt
    26221 taaacactta attgacaaag tatctgctat ttttataagg agtctttctt gtgctgaaaa
    26281 aaatcactga ttgaatggct ttgaattttc tacttagtaa aattattttt tcagcaattt
    26341 tcctgcaaaa cttttgaata aggaaaaaat acgagaaaat atcagtgatc tttcataata
    26401 ctgcccatta ttttcaggta tatttaaata taatatcact ttaggagaga acgaatttct
    26461 ttagagaaat aaaagttaga attttctgac ttgattttta caatgacctt aattaaaatc
    26521 gttctttcta catttcgtga ttaagcaaat atcaactaag tcagtagaac cattcacata
    26581 ggagggtgaa cattgaaaga tttttactca gtaaatgaat tagccgaaca attaggtgtt
    26641 actacacgtt ctatcagaaa ttatcttcat gaaggaaaac taaaaggaac aaaggttggc
    26701 gggcaatgga aattttctga acgtaatctt tttgagttcc tatacggaga tcaagcagag
    26761 gaagcagcaa aagaaatgca gcgattcatg ctaaatgcac caatcacgat gcgtttcaac
    26821 cttcaatatc gtgattttac agcaatcaat caattccgag aacaattagt ccagtaccat
    26881 aacgatgttt acgcaaataa aaaagaccgt cttttacaat acgatcttta taaagacaat
    26941 catgcggaga tactaatcgg tggtaacttc aattatgtcg taaactttag tcaatggatc
    27001 aatgaaaaat tattgatcca gacagatatt tcccttgtat cttaaacaat taaaaagcgc
    27061 tagaaattcc agagatgttg gaaattctag cgctattttt ttataagtac ttactgtttg
    27121 gttcaaacaa atattttttt aataaatgaa attttaccat ctttatcctc aaataaagac
    27181 cgtgaccatg cttcctacta cgatcagcaa taatccgata atcgttaatt ttttttcttt
    27241 tggtgtctta ttttcatgca ggatgtacat accgcctaat gtagagataa ctacgtttag
    27301 ctgtgtaatg atatatgcag tagcaacccc attttcattt gctgaaaaaa tataagccaa
    27361 tgctgacaca ctaaagatgg ctccaacgat cgttgctttc caactcttgt cttcagaaaa
    27421 tgcggttggt ttacggaaaa tcacataaat cactgcccct aaaaagacac cgatcatttg
    27481 cggaaagaaa atggaaagtc cgcttgcatc aactagttta ggcaatgcac tgtataccca
    27541 ataaccaata cttgttgatg ctaatagact aattcctttt gtgataccgc ctttttttcc
    27601 aggatcatca ctgattgccg ttaaagcaga accaaataca agaagaatga ttgctgctgc
    27661 tccaatgatt ttccccatac ttccagacca ttctccaaaa gcgaagacac caatcaacga
    27721 ggttccgact aactgtaatg cagtcgaaat tggcatcgtt ttagaaacac caatcaagtt
    27781 gaaagatgta tactgtcctg tttgtccaat aacccaaaat gcaccagaaa gtaaactaat
    27841 caaaaatgta gtcactgaaa taccagctgg agaagcgaat aattgaacga gtaagccgac
    27901 aagcaccgct ccaataccag ttcctaatat ctcatttgtt ggtctgccac caatcttttt
    27961 caaaatcagt ggctggatcc cccagccaac tgccgggatc aatgcaatca ataaacccat
    28021 ttttattcac ccttatcctt ttccaccttg gaaagaagga tataaagtca tcccaccatc
    28081 tgcaaatagt gtaatacctg taacataact ggattcgtct gaagccaacc atgcagctgt
    28141 cgccgctatt tcttttggtt cacctacgcg tcctagtggg atcatacttt ccaagagttc
    28201 tttttgttca ggatcatcaa attttgccgc attaataggt gtacggatag caccagggcc
    28261 tatactattg attctgattc ccttcggtgc atactccatt gctaccgttt cggtaaataa
    28321 ttttaccccg ccttttgaag cagcgtaatg agcaaacgtt ggccatggga tctgttcatg
    28381 aacagaagac atattgatga tatttccttt tttattattc tctgtaaagt aatttaaagc
    28441 cgctttcgtt ccgataaaaa cgccagtcaa attgactttt aacactcttt cccaatcttc
    28501 aaggctaagt tcatgggttg gatgctgatt ttccatccca gcattattaa cccaaacgtc
    28561 cagtgtccca aaattttcta aagccgtatc aagtaaacgc tgtattcctt cttcagaact
    28621 gacatctgct tgtacagaga ctgcttttcc gcctgcctct tcgattgctt tgactgcttt
    28681 ttctgctcct tctttatccg agttatagtt gacaactaca ttcatttttt ctttcccgaa
    28741 tcgttgagca atcgctgttc caatcccctt tgagccacca gttactactg cgactttgtt
    28801 ttctaattcc ttgtacatag tacccactcc tttttcatac ctaaacctgt tattacggat
    28861 aacttttatt tccgccttaa agtgagtgta caccaagata tatagtctaa agcaaatgat
    28921 acgctctagc aatcgttttc atgaattgct agtgtccttt tatttctata aagaaagagg
    28981 ttgtgacaga agtggtcagc ttcaagaaat ttcggcttat ttccgaagaa gctacttctg
    29041 aaaaaccatt tatttggaaa aaaaggctgt gacaagactt ttgtcacagc ccctttcctg
    29101 ggcttattta tcacccattt tattgtaatt gctagttata cataaacaag agattttatg
    29161 cataatgttt tattgctgag aaaaatattc agctgttgct tgttcatcct ccttgagttg
    29221 ttcaatcaat tcctctacac tagaaaattt cacttgatct cttaaaaatt gattccagcg
    29281 taccgcgaca tgttcaccat agatatcttg attaaagtcc aaaatataca actctacagt
    29341 cagctcccgt ccttcaccga atgtatcatt atgtccaatt gaacccatgg ctggatacca
    29401 tgtttcccca atttttattt ccgacacata aacaccttct ttaggcaaat gaaccgtgct
    29461 ctttactttg acatttgctg tagggaatcc tagtagtctg cctcgtgcat cgccatggac
    29521 aactacccct tctacctcat atgtgcaacc taatagtttt gctgcttctt ttacttgtcc
    29581 gctgtctagt aatccacgaa tacgggtaga gctgattttt tcaccttgtt cttcttcttt
    29641 agaaactgtg acgattttga atcttccttt ggcatattca ggaaaatgag cgacatctgc
    29701 aatgtctttt ggtccatacg tataatcaaa accggaaaca gcatattttg catgtaaccc
    29761 tacgatgtat tgatcaacaa attcctgcgg tgctaaatga gcaaaagaag aagtaaaatc
    29821 aacttcatat aaataatcta tacccaaagc ggccattttt tcttcttttt gcttaaggct
    29881 agtcaaatat ttcacgctgc taggatcgat ctttttaaag acaatagatg gatgctgatt
    29941 gaaagtcatc aatgctaatt tcaatccttc tttttgtgca atttccatac ctgtctttat
    30001 gactttctgg tgtccgatat gcacgccgtc gaaaaatccc agcactaaaa cgacatcgtc
    30061 ttctggaatt tgttctgatt ggtaaggatg tctgatcttg atgatattca ttcgttattc
    30121 ctcatttctc aatactttac taggttttaa tttgtttttt tctttcgggt ttggctgata
    30181 aatactgaca acttttcctt tataaaacaa agcaatttct tctgacggca tttctgggag
    30241 cgcaaataca gtataatcaa gtctcatacc gttttttact ttctgccata cttcatcagt
    30301 aatatctact cgtttgaacc gttccactcc tttttctatt ggcagcaaac aatcatcgat
    30361 tgtcccattt ttcacagctt gagctacttc ttctaatgtc agagcatcct tctgggacat
    30421 accggcactt gctgttcggg tcagatcaga catgtgcgca gggtaaccaa gtttcttgcc
    30481 tgtatctaca gctaatgtgc ggacataggt tcctttcccg cattctactt cgaagcgcca
    30541 agaaatggtt cctgtcactt cgtctcttgt caattcactt gtccggataa aacggtaaat
    30601 ctgagccttt ctaacaggac gctccactgt ttcattattg cgggcatatt catataaccg
    30661 cttaccgttg acttttaccg ctgagtacat tggtggtatc tgggtgatct caccaataaa
    30721 tgaagccatc acttcatcta tttttttttc agataaagac atatctactg gttttgtttc
    30781 tacccgttct ccggatcgat cttccgtcgt tgtactgaaa cctagagtta tctcgcctac
    30841 atagattttc ccagaatctg tcaaatattc gataactttt gtggcttttc caatacaaat
    30901 gggcaacaca ccgtctacgt ctgggtcaag cgtaccccca tgtccgattt ttttcgtatg
    30961 taagattttt cttaatttga atacgcaatc atgacttgtc attccgcgtt ctttccataa
    31021 aggtaataac ccgtccatgt cttttctcca tttcattttt gatttaactg aattattata
    31081 acacattgca tcaattactg acgaatttga catcataatg aacggttttt atttttcggc
    31141 aataaaaacg taacaaaatt catgctcacg taaatagaaa tcatgttatg cttttttaaa
    31201 ggagggaata gaatggaaga ttttttcttt gcaccatttg attggattta tgggtggctt
    31261 acaggtaaat agtttggttt tcacttccca aaaacgaaaa aaaagactgg ccattcttcc
    31321 agtctttttt ccgttttttt tatttatttt ttcagtttct taatcaatgt aaaaaattct
    31381 attttgcggg atcgatttta tcattttatt ggtcaagatt tttccatttc caatccaata
    31441 cttctggcag atcaatacca ttttcacgaa tatagttttg atgttcttgt aatttctgat
    31501 ccattttttt cgcaaaatct tctgcttgac ttcctttgat ttttaaagct gcgtcttttg
    31561 ccaaatgata acgatcaagt tcatttacta cacgcatgtc aaatggtgtc gtgatatctc
    31621 cattttcttt gtagccatga atcattaaat gatggttatg acgatcaaag aaaatcgtgc
    31681 ggaccaatgt ttcataaccg tggaaagcaa ataagatcgg tttgtctttt gtaaaatatt
    31741 gatcaaattc ttcatcgctc aacccacgcg gatcttgact tggatgacgt aatttcaata
    31801 aatctacgac attgatgaac cgaattttta gttttgggaa ttcttccagc agcaaagtta
    31861 cagctgctaa tgcttctaag ttagattccg ttcctgcagc agcaatgacc aattcaggtt
    31921 cctcctcttt tgctgtcgaa gcccaatcaa tgattttcaa gccttcattc actaagacgg
    31981 ctgcttcttc tgctgagtaa aattgagcac gcggatgttt ggaagagatg atcaaattga
    32041 ttttttctga cgagcggaat gctttatcca tgactgcgag caatgtattt gcatccgcag
    32101 gcagatattc acggatgaac tccgccttct tttctgccaa atgtgtcaat attcctggat
    32161 cttggtggct atatccattg tgatcttgct ggaagactgt tgagcttgca atgagattta
    32221 gcgaaggata atcatttcgc caagaaaggt catgtgattt ccgcatccat ttgaagtgct
    32281 gagtcaacat agagtcaacc actcgcaaga atgattcgta acttgcaaaa aaccatgccg
    32341 tccagtcaga acgtaccctt ctaagaaccc ttcatcttgg tgttccgata attgactgtc
    32401 gatgacgcga ccagatggcg aaagccattc atcattttct ggttcgattt tttccatcca
    32461 ttggcgattt gtcgttttga aaacttgatt caatcggttt gattttgttt catctggtcc
    32521 aaatatacgg aaattttctg gatttttctc aatgatctcg cgaacgaatt taccaagttc
    32581 ggtcatatct tctgctttaa cggagcctgg tttttcaaat tgcaaagtat attttttcca
    32641 atctggcata ataagcggtc ttggatcgat tccaccattg gtgataggat tttttgccat
    32701 tctttggtca ccagtaggcg cgatttctct gatttcttct aaaatacgtc cttgtgcatc
    32761 aaataatttt tctggttcat aggatttcag ccaacggagt aaagcatctg catgatccat
    32821 atgttcttga tctacaggaa tcggtacttg gtgtgctcgg aaggttcctt caattggttc
    32881 cccgtcccat tcttttggac cggtccatcc ttttggtgtg cgtacgatca atactggcca
    32941 ttttggcatc acgacatctg ttgctgtctt caggcgagct tctttttgga tggatttaat
    33001 tttctcgatt gcttgatcca ttttttcagc cataacagga tttaattttt ctgaatcatt
    33061 tccttcaata aagaaaggtt cccagccaag tccgttaaag taatttgcta attctccatc
    33121 tgatttacgt gccaagatcg ttgggttggc tatttttgca ccatttaaat gaaggatcgg
    33181 taaaacagct ccatctatca ctggattgat aaagacattt gagaaccagc ttccagctaa
    33241 aggaccggtt tctgcttcac catcaccaat cacgacagca gagattactt caggattgtc
    33301 taatacagct ccgactgcat gtgaaagaga gtatccaagc tctccgcctt catggataga
    33361 acctggtgtt tctggtgctg catgagaagc aatgcctcca ggaaatgaaa atcgtttgaa
    33421 taatttttgc attcctgtct catcttccgt tacttctgga tagatctctg tgtaacttcc
    33481 atctaaataa gcattcgata ccatgacttg tccgccgtgt cctggccctt caatataaaa
    33541 catgttcaaa tcatatttat tgatgactcg gttcaaatgg acataaataa aattttggcc
    33601 aggaatcgtt ccccaatgac cgattggatg ttttttgaca tcttcgggtt tcagtgtacg
    33661 tctcagtaag gggttatctt taagataaag ttgcccaaca gaaagataat tagcagcacg
    33721 ccaccaagcc gtcattttat caaagtattc tttactagaa taatccatac ataacactcc
    33781 ttttcattat tactcttatt ctagcgcaga tactcccaat tgaaacctaa taagcacttc
    33841 tgttcatttg tttggatttt atatctagcc aaatgaacag gtataaaaaa ccacgaaatt
    33901 tttcctttcg ctgacgtttt ttacattgat ccaaaaaact tgtcgttatc acaatggctg
    33961 ttccgcagtt catttcgctc ctcatcatgc ataatttgct gaatgacgct gggccagtta
    34021 acacattgct ggaaaactgg ggctggatcg agcaattcct ttcctgattg atccgtttta
    34081 tgtaaaagtc tctgttatct ttgtcaatat gtgaattggt atcccagcta ctatgctaat
    34141 ttctacaggg attatccaaa atttggatcg tgaccaaatc gaagcggcgt aatcggaaat
    34201 atcaataact tcaacgtgat ttttctttta actgtcaacg gaccgacaaa ctcaaacttt
    34261 tatggtgcag gttccactga tcttctagtt acttggctat acctgactgt cactaaaata
    34321 gattacaatc tagcttccgt tatcggtatc ttgatcttta tctatatgca aaaatttata
    34381 tactactaga aaaaagaaag tcgaattttt tacttttctt tttctctttt ttggttagct
    34441 gacacgccgc aagactaaag tcatggaact cttaggtaat acgtcttaat gaaaacaaaa
    34501 aaatcccgca acccctgatt tcacaggatt tgcgggagtt tttgattagt ctttgttcaa
    34561 gtcgcgaagc aattcatcga tatggttgcc atagacaact gattcatcaa gttcaaagaa
    34621 caattcaggt gtcttataaa tacttagatt gtgccctaac tctcgacgaa tcaatcctga
    34681 tgctttattc aatccatctt gtgctgtttt cttatctgat gctaagtcag ataaaatgct
    34741 gtaatagatc gttgcttgct gtaagtcccc cgtaactttc acatctgtga tcgttacgtt
    34801 ctccacacga gggtcacgga cctttttgcg caaaatatca ttgacttctt ttaagatttc
    34861 ttgaccgact cggcggtcac ggtagttagc cataatgatt ggcctccttt tatcagtttt
    34921 tagtcatttt tgacttcttc catgataaat ccttcaatta cgtcatctac tttgatgtca
    34981 ttgaattttt caatcattgc accacattca taacccattt tgacttcttt cacgtcatct
    35041 ttgaatcgtt tcagacttgc aagttggcct tcatagatta cgattccgtc acggattaca
    35101 cggacaccgc tatctctacg aatatatcct tctgttacat aggcacctgc aatcgtacca
    35161 actttagaaa ctttgaaggt ttcacgaacg atcatttgac cggtgatttt ttcttcaaat
    35221 tcagggtcta gcatcccttt catcgcagtt tcgatttctt cgatggcttt atagatgata
    35281 cggtgaagac gaatatcgac ttcttcagta tcagcttgca cttttgcctg cggtgtagga
    35341 cgaacgttga aaccgatgat aatagcgttg ctggctgctg ctaaggttac atcactttca
    35401 ttgattgctc cgacagctga atggacgatt tttacacgga caccttccac atcaatcttc
    35461 ttcaatgatg cagccagtgc ttctgctgaa ccttgtacat ctgctttgat gattacgtta
    35521 acttctttca attcgccttc tttcaggctt tcaaataagt tatccaaagt cacacggctt
    35581 gtggcagcac ggttttctaa taatgcacgt ttcgcgcgtt cttcacccgc tgcacgtgct
    35641 gtcttttcgt cttcgaagac tacgaaacgg tcacctgctt gaggtacatc attcaagcca
    35701 gtgatttcta ctggcgttgc aggtcctgct tctttatcgc gacggccaag atcgtttgtc
    35761 attacacgga cacgtccgta agtattaccg acaacgattg ggtcaccaac atgaagtgtt
    35821 ccttcttgga caagtaaggt tgacacagga cctttacctt tatcaagacg tgcttcgatc
    35881 actgttccga ttgcgcgttg tgttggatct gcttttaaat cttctacttc tgcgactaac
    35941 aggatcattt ccagcaattc ttcgatgttt tggccgaatt tcgctgagat ctcaacaaag
    36001 atcgtttctc caccccatgc ttcaggaatt aactcatact cacttaattc ttgcataaca
    36061 tgttgagggt ttgcacctgg tttatcaatt ttatttacag cgacgatgat tggaacgcct
    36121 gcagctttgg catggttgat tgcttcaact gtttgtggca tgacgccatc atctgcagca
    36181 acaactaaga tcgtgatatc tgtgatactt gctccacgtg cacgcatact tgtaaaggcc
    36241 gcatgtcctg gtgtatccaa gaaagtgatt ggtttaccat caatatcgat ctggtaggca
    36301 ccgatatgct gtgtgattcc gccggcttcg ccgcttgtta cacgtgaatg gcgtaatgta
    36361 tccaataaag ttgttttccc atggtctacg tgtcccataa tcgttacgac tggcggacgt
    36421 gagactaatt tatctggatc gacttcttct gcctcgaaga atttatcgat atcagcaatg
    36481 tcaacttgga ctttttcttg tggttccatg ccatagtccg tcgctaacag ttcaatcgta
    36541 tctttatcca gtgcttggtt ttgattgacc atgacaccaa gcataaataa ttttttaatg
    36601 atttctgctg gctcacgatg aatttttttc gcaatatctg ctacgttcat accttcagta
    36661 tactctaata catctggcaa ttcacggaat ttgcgtggtg gtacagctgg tttagttgat
    36721 tgttgctgtt tgcctttttt acctttttta ttataacgat tacggttatt attattaaat
    36781 ttgccgcgat tgttattgtt tggtttgttg ttattgttag tacggttttg attgccgcct
    36841 tggctcgttc cttgacgatt ttggctgctg gtatttcccg tattattttg gtttgttgtt
    36901 ggtttttttc cttgattcac ttgaccgctc ccttgaccgc gatcttgata actgcggtta
    36961 tttttatttt tttgttgatt gttcgttgtt tcttttttac gttgatcgtt cggctgttga
    37021 ttggctggtt ttctgcttgc ttgttgtgca gcttgttgtt tcgtgcttgg gtttttgaac
    37081 gcttgttgca atttcttctc atcattgcta gtaatcgcac ccatatggtt tttcacatcg
    37141 atgccaagtt gttgtgcttt ttccacgacg tctttactag attttttgag ttcttttgct
    37201 aattcataga ttcttttgtt gcccatgcaa tcaccttcct aaccttcaat tagctccttg
    37261 gtcttcttcg caaatccggc atccattatt ccgaatacct tgcgaggttt tccgatcatc
    37321 tgcgtgattt ctgcttcaga aaatagctca aagcaaggaa cttcataata tgaactctta
    37381 tcttttatct tttttcttgt attctcgctt gcatcggttg ccacaaacac caattttgct
    37441 ttttgtttgc ggatttcagc aatggtcagt tcttcacccg tgattagttt tcccgcgcgc
    37501 atcgctagac cgatgagatt catggctttt tgccgattaa ctgtattcat tttccaaata
    37561 actctttacg tgctttttgg tgagtgacat agtcaagaag ttcttgataa aactcatctg
    37621 ttaatgttgt ttcgagtact cgatcgagaa tttttttatc ccaagcttgt tgtacttctt
    37681 ccggctcaag tgagacatac gctcctcgtc caggcatttt tccagttgga tcgatcgata
    37741 cttccccttc ttttgaacgg gtgattcgga ccatctcttt tttaggtttc atttcacctg
    37801 aaaccacgga tttgcgtaaa ggaattttcc tttgtttcat ctctttgcct ccttactaaa
    37861 gttaagcttc tggtttttct tcgaccgttt cattgttgaa tgcaattgtt tcatattcat
    37921 cgtctgttaa atctgactga acgattgctt catcatgcag ttcttctgtt tctttttgtt
    37981 gttcttgatt ttcataaaat tccgtcatgt cagattctga tttgatatcg attttatggt
    38041 tagtcaattt agcagctaaa cgtgcgtttt gtccgcgttt accgattgct agcgataatt
    38101 ggtagtctgg aacgacaact gtgcaggcct ttggatttgt ctcatcgaag atcacatcca
    38161 tgacttgtgc aggattcaat gcatttgcaa tgaatacagc tggatcttca ttccattcga
    38221 cgatatccat gttttcgcct tttaattcat tgacgattgc ttgaactcgt tgtccctttg
    38281 gcccgacaca tgtaccgact gcatcgatat ttggatcatt tgagcgaact gccacttttg
    38341 aacgatcgcc agcttcacgg gcgatgctga caatttcaac gattccatca tagacttctg
    38401 gtacttcttg ctcaaataag cggcgcaata ggtctggatg gctgcggcta acaaatactt
    38461 gcggaccttt agacgtattt tctactcggg aaacataaac tttgattcga tcatgtggtt
    38521 gataaaattc atttggcatt tgatcttgtt tagaaagaac tgcctcaatt ttaccaagat
    38581 tgacataaat ataacgttta tcctggcgtt cgacgattcc ttgcatgatg tctttttcat
    38641 aagcactgaa ttcgttataa ataatcgttc tttctgcttc acgtacacgc tgtaaaataa
    38701 cttgtttagc cgtttgcgca gcgatccgcc caaaatcttt tggtgttact tcaaaacgga
    38761 tcgtatcacc gatctcatat gctggattga ttaaaagtgc atctttcaat gagacttcca
    38821 actgtgaatc catcacttct tccgttactt cttttaccgc atagacatgg atctttcctt
    38881 ttttctgatc aaattccact tcgacgtttt gtgcttggcc atagtgacgt ttgtatgcag
    38941 aaaccaatgc tgcttctaaa gcatcgatca cgatttcttt tgagatccct ttttcagctt
    39001 ctaaagcgtc taacgcattt aacatttctt tactcatttt ttctgattcc tctccgtgat
    39061 taaaattgga tagctaagcg agcttttgca atattttttc ggtcaaaagt caattctttt
    39121 tctcgtgttt ttatgcgtat tttcaatgta agctgttcag catcaaatga ttgaagaaat
    39181 ccttcatatt gtttttcgcc atctactggt tggtataagg agacatggac ataatcacca
    39241 agtgcctttt catagtcgct ttcttttttc aatggccgct ctgctcccgg ggaggatacc
    39301 tccaggaaat aagcttgagg aatcggatct ggatctgctg catcaagttt ttcactcagt
    39361 ttctcactga caaaggcgca ttcttcgata tcgataccgc cttctttgtc aataaatact
    39421 cgtaagaacc agctttttcc ttcttttaca aattcgactt cgacaagttc aaaattttgt
    39481 tcatctaaaa tcggtgtcac catttctgtg actgtttcaa cgacgctact caaaagtttc
    39541 gcctccttaa aaaattaagt gcgtaattcc attaaaaaga gtgagcgaaa aatccgctca
    39601 ctcacagtta gtatcattac ctataagaac tataccatac tctgcttaaa attacaagca
    39661 gacaggaaaa cagatgctta tagcatatcg aataacgata attgattttc atctggtaga
    39721 tctttcaata cgccattgtc gttcatatat tcgatcaatg tttttgaaac tttaccgcgg
    39781 gtggcaagat cttctttgga taaaaatgga ccgtcttttc ttgcttcaac gatttgtttt
    39841 gccacgttgg ttcccaaact tgggacagct ctaaatggtg cgatcaacgt atcaccttca
    39901 ataacaaagt taacggcatc cgatttatac aaatcaatca ttccaaattt gaatcctcgt
    39961 tccagcattt cattggctaa ctccagcact gtcagttgat tcttttcttt tacggaagca
    40021 tctaatcctt tgtccgtgat ttctttcatt gcttgtttca cagcatcttt tcctttgcac
    40081 atggagacta gatcaaagtc gtctgcacgt acagaaaagt atgcacagta atacaaaata
    40141 gggaaataaa ccttgaaata agcgacacga agagccatca atacgtaagc tgcagcatgg
    40201 gctttcggaa acatatattt gatttttgaa caagaatcga tataccagtc gggcacatta
    40261 ttttctttca tcgccgatag ataggtttct cgcaattcat cagggatctt gttccattgt
    40321 cctttacgaa cagtttccat gattttaaat gccatcccgc tatctaatcc agcatggatc
    40381 aaataaacca taatatcatc acgacagccg attacttccg ccagtgtcgc atctcctcgt
    40441 cgaatcaatt cttccgcatt tcctaaccat acgtctgtac catgagatag tccggaaatt
    40501 tgcagtagtt ctgcaaaagt tgtaggatgc gtttcttcta gcatccctcg aacaaaacga
    40561 gtgccgaatt ctggaatacc taatgtccct gttttggaat aaatttgttc ctgacttact
    40621 ccaagtactt caggaccggc aaatatccgc atcacttccg ggtcatccgt agggatcgtt
    40681 tgtggatcga tccctgataa atcttgaagc atccgaataa cagttgggtc atcatgccct
    40741 agaatatcta atttcaatac attatcgtga atcgagtgaa aatcaaaatg agtcgttttc
    40801 cattccgaat tttgatcgtc tgctggatat tggatcggcg taaaatcata gacatccata
    40861 taatcgggaa taacgatgat cccccctgga tgctggccgg ttgtccgttt gactccagta
    40921 gcgccttttg ctaatctgtc gacttctgca cttcgaaact gcagattatt gtccctttcg
    40981 tatcctttta catagccata tgctgttttg tcggcgactg taccaatcgt tccggcacgg
    41041 taaacatagt cttcgccaaa caatactttc gtatagttat gggcttctgc ttgataatca
    41101 cctgagaagt tcaaatcaat atcgggcact ttgtctccat ggaatcctaa aaatgtctca
    41161 aatggaatat catgaccgtc tttgtttaag cgtgcaccac attttggaca ttgtttttct
    41221 ggcatgtcaa atccagaacc atacgtacca tcttcaaaaa actcggaata ttggcactct
    41281 ggacaataat aatgaggggc taacggatta acttcagtga ttcctgtcat cgtcgcaaca
    41341 aagctagagc caacagagcc acgtgaacct actagatagc catcttcatt acttttatgc
    41401 actagttttt gggcgatcag ataaataacc gagaagccat taccattaat agaattcaac
    41461 tcttttttta atcgtttttg aatgatctca ggtaacggat ctccgtacat ttgttttgct
    41521 tttgtgtaac tcaattctga tatttcatct tctgaacctg gaatcttcgg tgtataaagt
    41581 tcatctttga ctggaatcac ttcttcgcag atatctgcga ttttattcgt attttctact
    41641 acgatctctt tcgctgtttc ttcccctaaa aagtgaaatg cagttagcat ctcatcggtt
    41701 gttcggaaat ggacatccgg caaactatga cgattcaacg gattcgctcc gcccatagaa
    41761 ttgatcaaaa tttttcggta aatggcatct tcttcgttca gataatgtac attacctgtt
    41821 gcaacaacga ttttatccaa actttttccg atttctacca aattttggat aatttcttcc
    41881 aaatcatgtt cattttttac caattcttgc tcgatcaaag gcgcatagac cgcttttggc
    41941 ataacttcaa tataatcgta gaattttgct cggtttcttg cctcttctac ccctttttgc
    42001 atcatggctt cgaatatttc tcccttatca caagctgagc cgatcaatag attttcacgc
    42061 atctttttca attgggagcg tggtattcgt gggacgcgtt cgaagtattc gacattcgac
    42121 attgaaatca gtttgaacaa atctttcagt cctgcctgat tttttgctaa aatcgttaca
    42181 tggaaaggtc gagccctttt gtaagaatct ccttctccga tatgctcatt tagctgatca
    42241 tgataaagca tgttatgatt gtccattgcc tctttcacaa agatccatgc taagtgacca
    42301 gttgcttcag catcatagat tgctcggtga tgctgttcca agctgacacc aaatttttta
    42361 gataacacac ctaatccgaa acgtttgaat tgaggataca gatatctagc taattccaac
    42421 gtatcaatga ctggattggc cgcttcagga ataccatacc ttgcgtagct agtattcaag
    42481 aaccccatat caaaagccgc gttgtgggct accaaaattg tatcttttga aaattcaagg
    42541 aacatccgca atacttcttc ttccgacttt gatcctcgaa ccattccatc cgtgatacca
    42601 gtcagatcta ccgttgttcg tgacaaagga tgtcctggat cgataaattc atcaaagctt
    42661 tcgatcacat ttcctttata cattttgact gcggctagct cgatgatcgt gtcatatact
    42721 gcagacaatc ctgtcgtttc cacgtcaaat acgacatatg tggcttcact caatgcttca
    42781 tgtgcatcat tataagcaat tgggacacca tcatctacga cattcgcttc cacgccgtat
    42841 aagattttca cacctgcttt ttttccagca ctatgtgctt ccggaaaggc ttgcgcacca
    42901 ccgtggtctg taatggcgat agctcggtgt ccccatttcc ctgcttgtgc cacaagatca
    42961 gagatgctgt ttgttgcatc catcgtactc atattgctat gaacatgaag ctctactcgt
    43021 ttttcccctt caggagcata atctttgcga ggtgtatgtt tgacttctat gatatcttgc
    43081 gcattcatga ccaaatcacg gacaaacgta tcttcttgaa tacttcctcg tacctttaac
    43141 cagctaccga cactgatcgc atcaaatact tgttcatctt tttcgccgtt cgagaatttt
    43201 ttgacgataa atgatgaagt ataatctgtg attttcaaag tcaagatctt gcgttttgaa
    43261 cgtagttctc tgacttcttt gtcgaatacg tacccctcaa gtgtgactct tcgttcttct
    43321 tcaacgatac tgatcattgg tgtcgttggt tcatcggctg gaatatttcg tcctagctgg
    43381 atgtggatgg gtccttcaag agctggcgat tgctcttttc tttccttttt cttttgttcg
    43441 tggacaacta gactttcagc tgcttgcttc ataaaggctt ctgcctgttc ttgtttgcgt
    43501 tcttcaaaaa gtttcaatac tcgttccgct tgctgctcat ccacttctgg ttcgatacga
    43561 aattttggga atccgtaact gacgaacaac tcttctacta atggcaagta ttgttgcttc
    43621 aaataagaaa tagctcctgt actgtcgatt ggcaagatga ctttacgatc ttttatcaca
    43681 ggtacctgcg ttttcaatac ttgctgtgct agtggtgtat cgcattgctg attttccaaa
    43741 gcttgggccc agtaatcttg caagagctgt tcatcgaatg tctggtcatc tgctaagata
    43801 ttcagtttga cttgtgcaat atctttaaaa gcgatagtca gatgctgagt caatgaccga
    43861 tatagcataa taggcaaaat tttggaaaaa tgcagcgtaa attcccataa acgcgattta
    43921 cgatggacca ctacactatc catttcacca gcagtaagca gtggatgatt tttttctgct
    43981 tcctctagtt ggatttgttc caacagtttt tcaaataaat ctcgtttttc tgacaaagat
    44041 atacgccctt tctgatgaga aacaatgcaa ttgatcgcat cttatcctct tctttttcag
    44101 taccttttag tatactcgtt ttttctgagt tttacattaa aaaattggaa ctgcgattca
    44161 aatcgcagtt ccaattttaa aagcgaatca tcagattgtc aaaaacagct gattcagcta
    44221 tttttatttt ctgtgtttta acgcttatta ttcgccttgg ttcaagagga ttggtaaagt
    44281 attcaccaat tcttctttac gtacttcaac catttcgcca gttttcttga ttttcacttc
    44341 tacgattcca tccactgctt ttttaccgac agtgattctg attggacagc caatcaaatc
    44401 agaatccgcg aatttcactc ctgcgcgttc atttctgtca tcaaccagta cttcatagcc
    44461 agcttttgtc atcgcttctt ctagttcatt cgtcaggttt gtttggtatt cgtcttttag
    44521 attcatttgg acgatatgca aatcaaatgg cgcaattcct tttggccagt tgattccttg
    44581 ttcatctgaa tgttgttcta cgattgctga taataaacga cttacaccga taccataaca
    44641 gcccataatc acaaattttt ctcgtccatt ttcgtctagt acagttgctc ccatagattc
    44701 actgtaacga gtgccgattt tgaagatatg tccgatttcg attcctcgag taaaggcaag
    44761 aacaccattg ttgtctggtg aaggatctcc ttcttgtaca aaacgaagat cttcataaga
    44821 aagtacagtg aaatcgcgtt ctggattcac atttttatag tggaatcctg tttcgttcgc
    44881 tccagtaatg gcattcgcta aatcttggac ataacggtct gcatatactt ttacgtcatc
    44941 tgaaacaccg actggtccga cagaaccaaa ctcagcacct agatattttt gtgcttcttc
    45001 ttctgttgct tcttctaaaa agtcagcaga taaaaagttt ttcagtttta cgtcattgac
    45061 ttcatggtct ccgcgtacca aaacaaggac aggttgttca tccgcaatga acaatacaga
    45121 cttgatgatt ttttgaggtt caacagaaaa gaaatctgct acttcgtcaa ttgattttac
    45181 gttaggtgta gctactttct ccaattcaag ctgtgtttca tgcgattttt ttgatacata
    45241 gtagcttgta gccatctcca agtttgcagc ataatcactt tcagtcgaat aacagatcgt
    45301 atcctccccg atttctgaga tcgccatgaa ttcttttgag tctttcccgc ccattgcgcc
    45361 accgtctccg ataatcgagc gaaattctaa gccacaacgc ttgaatactt cggtgtaggc
    45421 tttttcgtaa tctttatatg tctcgtccaa actttcttcc gttgcatgga aagaatacgc
    45481 atctttcatg ataaactctc ttccgcgaag caaaccgaag cgaggtcgtt tttcgtcacg
    45541 atatttaggt tggatttgat aaagatttaa aggtaatttc ttatatgaat tgatttcatt
    45601 acgcatcaaa tcagtaaaag tttcttcgtg agttggtcca aggatcattt ttcgatcatt
    45661 gcggtctttg aaacgataca agtttggtcc atatgtttcg taacgcccgg attcttccca
    45721 aagttctgct ggcaagatgg caggcatcag catttctacc gctccgattt tttcaaattc
    45781 ttcccgcata atgcgtttca gtttttccaa tacacgattt gctaatggca gatatgaata
    45841 aatacccgct gatacttggc ggatatatcc agctcttagc aagatttgat ggcttaatac
    45901 ttctgcatca tttggaactt cgcgtaatgt tggaatcaac atttttgatt gtctcattct
    45961 atatactcct ttatttcctc aaaatagtta tcgtttgtta ttatacccta tttgtcaact
    46021 ttttctagaa aaagaatcgt tgaatatcgt tccatgtaac tagcaccatc aacaacatta
    46081 aaaatccaaa gccagctaat gtcaatattc cttctttttc ttgacttaat ggtttcccgc
    46141 gaatcccttc aaaaatattc aagactaatt tcccgccatc taaggcagga atcggaagca
    46201 gattgacgat tccgagattc attgaaagaa gcgccatcaa actaataaca gttgttacac
    46261 cttgatttgc tgcttctgaa gacaattggt acatcatgac aggtccgcct aatttatcta
    46321 aactgaaacc tgtaaatagt gatcctaagg ctttgaaaat ttccaatgaa ccgctgaaag
    46381 cttgacgcgt ccctccaatg atcttatcca taaaacctgt attcattggg gcttttatac
    46441 caagttggcc aactttttcg ccatttgact ctacagattt cggtgtcacg tcgacagatt
    46501 gcacctgacc ttctcgttcg atcttaaagt ctaatgtttt atccggattt ttcgtaatga
    46561 ctgttgtcag atcattccat gaatggattt cttttccgtc cacactgact acctcatcat
    46621 tttcttttaa gcctgcttct gccgcagctc cattaggcat gatctctcct acgcgattag
    46681 tgttcgtcac ttgaacacca ccttgcataa atgccagaat gatgaataac aggattgcca
    46741 aaataaagtt attcatgggt ccagcaaagt tggtcagcat acgctgccac aatttagccg
    46801 attggaactg tacgtctttt ggcgcaattc tgatttctgt tccgtcgttt tcaataatcg
    46861 tcgcatcgtg atcaacaggg taggttacta cttctgtttc gtctccattc acataaccag
    46921 tgatagtcag ctcatcttct aaatcataac ggctcaattc catcgggata gcatttgtca
    46981 attggatttt cttacttaaa tttatcttct ctacgatgcc gtcagaattt agtaaaagag
    47041 aaagaggcat tcctggtgcc atttctgttt catcatcccc attgcctgcc attcgaacat
    47101 aaccgccaat aggtaatagg cgaagcgtat atgttgtccc gtcttttgct tgatgcccat
    47161 aaattttcgg acccataccg atagcaaatt cgcgaacgag gattcctgat cgtttcgcaa
    47221 agaagaaatg accaaactca tgaacaatca ctaatatacc aaaaacgatg ataaatgtca
    47281 gaatcgtttt catagctttt ttctccttta cttgtttttt gtgtttttta taaaacaacg
    47341 ataaaacact ataattttac catagtatac caaagacacc aatagtatca atataatgaa
    47401 aaataaaaac ctgcgtcttt tatgaaattc acaaagaaac gcaggtatac ttactaaata
    47461 atgccaaata aatgcatgat aggaaataca aacagcaaac tgtcgaaacg gtctaagatt
    47521 ccaccatggc ctggtaagat atttcccgag tctttcacat cataatgacg tttgattgca
    47581 gattcgacca aatcaccaaa ttgtccgact gctgaaaaaa taatcgtcat cagaagcatg
    47641 acaaacatac catgattaaa tagttctttt gctgggtata gcaataaata aagaaatgcg
    47701 actacaacag cactaccaat accaccgagc gcaccttcaa tcgttttgtt gggcgaaata
    47761 tcaggactta acttatgctg accataacgt cgaccaacca tatatgctcc gatatcagtt
    47821 gcccaaacaa taaatagacc aaatatcaaa acagccaagc tttctgttcg tgcattgaca
    47881 aaattctgga aacctattcc cacatacaag ctgacaagca caggaaaacc tgcttcatca
    47941 atcgtatacg tatttttaga aataactgat gttcctaaaa gaatcataac tataagataa
    48001 aataacataa atgtacttac attctctgga agaaagaaaa accatctttc ttttggcaaa
    48061 acaagtaaca ctgcaccaac ggcggataaa actccttcaa aacttaagag tgtcaatcct
    48121 ttcatccgaa acagctcgta aactcctatt actgctaaga ttgctgctgt aagctcaacc
    48181 gctatgcctc cgatccatat aatcggaata aacaagacta gcgccacggc tgctgtgacc
    48241 acacgttgtt tcatgactga tcccctttct catcttctgt tttttttaca ccgccgaatc
    48301 gacggtctcg attttgataa gaggcgattg cttcttcaag atgcgcacca tcaaaatcag
    48361 gccaaagcgc ttttgtaaaa tacagctcac tatatgcgat ctgccataac agaaaattac
    48421 taattcgctc ttctccactt gtacggatca tcagctcagg atcttgtaac tctttaggca
    48481 agaaacctgt cattaaatgc ttagcaatca attcatcatc gatttcttct gcgtgtattt
    48541 ctccttttgc tacttcttcg gcaatttctt ggacagcagt cacgatctct gctctgcttc
    48601 cataattcaa agcaaaattc aataccattc cagtattgtt ttttgtttgc tcaattgcac
    48661 gtcttacagc atcttgcgta tgttctggca atacgttttc ataacccatg acatgaactt
    48721 tgacattttc tttaatcaat tctggtacga acgtatcaaa gaagtcgaca ggtagttgca
    48781 tgagaaaact tacttcatct tttggtcgct tccagttttc agtcgaaaag gcataaagcg
    48841 tcaacacttt cactccaagc cttgatgctt tttttgtgac tttctttact gtttccatgc
    48901 cttctttatg gccagcaact cgtggcaaac gacggttttg cgcccaacga ccattgccgt
    48961 ccatgatgat ggcaatgtgc tgaggaattg gtccatcggt attaaattga aattcgcttt
    49021 tttcttgaat atatttgttt ttttgcggaa aaaaacgtaa cattcttttt cctcctatac
    49081 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
    49141 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
    49201 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
    49261 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
    49321 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
    49381 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
    49441 ttttgcattt tctgcttctt ttttaacatc tttagctaat tcttttctgc gttcttctgt
    49501 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
    49561 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
    49621 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
    49681 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
    49741 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
    49801 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
    49861 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
    49921 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
    49981 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
    50041 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
    50101 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
    50161 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
    50221 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
    50281 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
    50341 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
    50401 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
    50461 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
    50521 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
    50581 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
    50641 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
    50701 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
    50761 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
    50821 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
    50881 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
    50941 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
    51001 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
    51061 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
    51121 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
    51181 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
    51241 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
    51301 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
    51361 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
    51421 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
    51481 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
    51541 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
    51601 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
    51661 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
    51721 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
    51781 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
    51841 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
    51901 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
    51961 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
    52021 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
    52081 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
    52141 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
    52201 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
    52261 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
    52321 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
    52381 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
    52441 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
    52501 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
    52561 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
    52621 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
    52681 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
    52741 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
    52801 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
    52861 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
    52921 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
    52981 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
    53041 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
    53101 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
    53161 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
    53221 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
    53281 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
    53341 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
    53401 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
    53461 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
    53521 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
    53581 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
    53641 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
    53701 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
    53761 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
    53821 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
    53881 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
    53941 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
    54001 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
    54061 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
    54121 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
    54181 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
    54241 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
    54301 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
    54361 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
    54421 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
    54481 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
    54541 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
    54601 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
    54661 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
    54721 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
    54781 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
    54841 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
    54901 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
    54961 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
    55021 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
    55081 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
    55141 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
    55201 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
    55261 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
    55321 taacagaata cattttgtac gctgacatga atccagaatg acttttatac aaaataagag
    55381 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
    55441 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
    55501 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
    55561 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
    55621 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
    55681 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
    55741 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
    55801 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
    55861 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
    55921 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
    55981 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
    56041 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
    56101 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
    56161 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
    56221 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
    56281 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
    56341 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
    56401 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
    56461 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
    56521 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
    56581 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
    56641 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
    56701 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
    56761 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
    56821 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
    56881 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
    56941 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
    57001 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
    57061 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta ctgtttgcaa
    57121 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
    57181 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
    57241 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
    57301 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
    57361 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
    57421 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
    57481 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
    57541 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
    57601 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
    57661 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
    57721 tagtcgtttc ataaaaaaac aatccagatg gcctaaagga gaagaaatac cacctggata
    57781 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
    57841 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
    57901 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
    57961 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
    58021 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
    58081 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
    58141 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
    58201 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
    58261 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
    58321 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
    58381 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
    58441 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
    58501 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
    58561 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
    58621 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
    58681 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
    58741 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
    58801 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
    58861 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
    58921 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
    58981 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
    59041 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    59101 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    59161 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    59221 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    59281 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    59341 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    59401 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    59461 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    59521 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    59581 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    59641 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    59701 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    59761 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    59821 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    59881 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    59941 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    60001 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    60061 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    60121 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    60181 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    60241 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    60301 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    60361 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    60421 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    60481 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    60541 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    60601 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    60661 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    60721 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    60781 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    60841 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    60901 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    60961 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    61021 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    61081 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    61141 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    61201 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    61261 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    61321 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttatt
//
LOCUS       DAZKOR010000003        60310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000003 DAZKOR010000000
VERSION     DAZKOR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.003"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..1411
                     /gene="nhaC"
                     /locus_tag="ACG8ZM_000120"
     CDS             44..1411
                     /gene="nhaC"
                     /locus_tag="ACG8ZM_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0491118.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1692..2711)
                     /locus_tag="ACG8ZM_000121"
     CDS             complement(1692..2711)
                     /locus_tag="ACG8ZM_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0491119.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(2733..3740)
                     /locus_tag="ACG8ZM_000122"
     CDS             complement(2733..3740)
                     /locus_tag="ACG8ZM_000122"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0491120.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="ACG8ZM_000123"
     CDS             complement(3733..4398)
                     /gene="pgmB"
                     /locus_tag="ACG8ZM_000123"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0491121.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(4391..6685)
                     /locus_tag="ACG8ZM_000124"
     CDS             complement(4391..6685)
                     /locus_tag="ACG8ZM_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0491122.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            6945..9110
                     /locus_tag="ACG8ZM_000125"
     CDS             6945..9110
                     /locus_tag="ACG8ZM_000125"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0491123.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            9414..10247
                     /locus_tag="ACG8ZM_000126"
     CDS             9414..10247
                     /locus_tag="ACG8ZM_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0491124.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(10349..12049)
                     /locus_tag="ACG8ZM_000127"
     CDS             complement(10349..12049)
                     /locus_tag="ACG8ZM_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491125.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            12556..13716
                     /locus_tag="ACG8ZM_000128"
     CDS             12556..13716
                     /locus_tag="ACG8ZM_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0491126.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13732..14175
                     /locus_tag="ACG8ZM_000129"
     CDS             13732..14175
                     /locus_tag="ACG8ZM_000129"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0491127.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(14658..15188)
                     /locus_tag="ACG8ZM_000130"
     CDS             complement(14658..15188)
                     /locus_tag="ACG8ZM_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0491128.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            15293..15844
                     /locus_tag="ACG8ZM_000131"
     CDS             15293..15844
                     /locus_tag="ACG8ZM_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0491129.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(15997..16470)
                     /locus_tag="ACG8ZM_000132"
     CDS             complement(15997..16470)
                     /locus_tag="ACG8ZM_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0491130.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            16608..18080
                     /locus_tag="ACG8ZM_000133"
     CDS             16608..18080
                     /locus_tag="ACG8ZM_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0491131.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            18077..18787
                     /locus_tag="ACG8ZM_000134"
     CDS             18077..18787
                     /locus_tag="ACG8ZM_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0491132.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            19139..19423
                     /locus_tag="ACG8ZM_000135"
     CDS             19139..19423
                     /locus_tag="ACG8ZM_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0491133.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(19740..20123)
                     /locus_tag="ACG8ZM_000136"
     CDS             complement(19740..20123)
                     /locus_tag="ACG8ZM_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0491134.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(20125..20709)
                     /locus_tag="ACG8ZM_000137"
     CDS             complement(20125..20709)
                     /locus_tag="ACG8ZM_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0491135.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(20756..22231)
                     /locus_tag="ACG8ZM_000138"
     CDS             complement(20756..22231)
                     /locus_tag="ACG8ZM_000138"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0491136.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            22412..22723
                     /locus_tag="ACG8ZM_000139"
     CDS             22412..22723
                     /locus_tag="ACG8ZM_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491137.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(22838..25174)
                     /locus_tag="ACG8ZM_000140"
     CDS             complement(22838..25174)
                     /locus_tag="ACG8ZM_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0491138.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            25316..25984
                     /locus_tag="ACG8ZM_000141"
     CDS             25316..25984
                     /locus_tag="ACG8ZM_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0491139.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            25987..27030
                     /locus_tag="ACG8ZM_000142"
     CDS             25987..27030
                     /locus_tag="ACG8ZM_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0491140.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(27267..28148)
                     /locus_tag="ACG8ZM_000143"
     CDS             complement(27267..28148)
                     /locus_tag="ACG8ZM_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0491141.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            28307..29035
                     /locus_tag="ACG8ZM_000144"
     CDS             28307..29035
                     /locus_tag="ACG8ZM_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0491142.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            29191..29508
                     /locus_tag="ACG8ZM_000145"
     CDS             29191..29508
                     /locus_tag="ACG8ZM_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0491143.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            29501..30109
                     /locus_tag="ACG8ZM_000146"
     CDS             29501..30109
                     /locus_tag="ACG8ZM_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0491144.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            30164..30955
                     /locus_tag="ACG8ZM_000147"
     CDS             30164..30955
                     /locus_tag="ACG8ZM_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0491145.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            31093..31581
                     /locus_tag="ACG8ZM_000148"
     CDS             31093..31581
                     /locus_tag="ACG8ZM_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0491146.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            31632..32504
                     /locus_tag="ACG8ZM_000149"
     CDS             31632..32504
                     /locus_tag="ACG8ZM_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0491147.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            32680..33042
                     /locus_tag="ACG8ZM_000150"
     CDS             32680..33042
                     /locus_tag="ACG8ZM_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0491148.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            33051..33803
                     /locus_tag="ACG8ZM_000151"
     CDS             33051..33803
                     /locus_tag="ACG8ZM_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491149.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            33817..35619
                     /locus_tag="ACG8ZM_000152"
     CDS             33817..35619
                     /locus_tag="ACG8ZM_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0491150.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            35896..36936
                     /locus_tag="ACG8ZM_000153"
     CDS             35896..36936
                     /locus_tag="ACG8ZM_000153"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0491151.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(36988..39132)
                     /locus_tag="ACG8ZM_000154"
     CDS             complement(36988..39132)
                     /locus_tag="ACG8ZM_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0491152.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(39489..40862)
                     /locus_tag="ACG8ZM_000155"
     CDS             complement(39489..40862)
                     /locus_tag="ACG8ZM_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0491153.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(40862..41257)
                     /gene="rbsD"
                     /locus_tag="ACG8ZM_000156"
     CDS             complement(40862..41257)
                     /gene="rbsD"
                     /locus_tag="ACG8ZM_000156"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0491154.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(41285..42202)
                     /gene="rbsK"
                     /locus_tag="ACG8ZM_000157"
     CDS             complement(41285..42202)
                     /gene="rbsK"
                     /locus_tag="ACG8ZM_000157"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0491155.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(42310..43323)
                     /locus_tag="ACG8ZM_000158"
     CDS             complement(42310..43323)
                     /locus_tag="ACG8ZM_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0491156.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(43404..44003)
                     /locus_tag="ACG8ZM_000159"
     CDS             complement(43404..44003)
                     /locus_tag="ACG8ZM_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0491157.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            44189..44890
                     /locus_tag="ACG8ZM_000160"
     CDS             44189..44890
                     /locus_tag="ACG8ZM_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491158.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            44900..47611
                     /locus_tag="ACG8ZM_000161"
     CDS             44900..47611
                     /locus_tag="ACG8ZM_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0491159.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            48062..49690
                     /gene="treC"
                     /locus_tag="ACG8ZM_000162"
     CDS             48062..49690
                     /gene="treC"
                     /locus_tag="ACG8ZM_000162"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0491160.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(49750..50931)
                     /locus_tag="ACG8ZM_000163"
     CDS             complement(49750..50931)
                     /locus_tag="ACG8ZM_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0491161.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            51183..52706
                     /locus_tag="ACG8ZM_000164"
     CDS             51183..52706
                     /locus_tag="ACG8ZM_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0491162.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(52775..53503)
                     /locus_tag="ACG8ZM_000165"
     CDS             complement(52775..53503)
                     /locus_tag="ACG8ZM_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0491163.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(53516..54796)
                     /locus_tag="ACG8ZM_000166"
     CDS             complement(53516..54796)
                     /locus_tag="ACG8ZM_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0491164.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(54937..55539)
                     /locus_tag="ACG8ZM_000167"
     CDS             complement(54937..55539)
                     /locus_tag="ACG8ZM_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0491165.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            55784..55990
                     /locus_tag="ACG8ZM_000168"
     CDS             55784..55990
                     /locus_tag="ACG8ZM_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491166.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            56302..57021
                     /locus_tag="ACG8ZM_000169"
     CDS             56302..57021
                     /locus_tag="ACG8ZM_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0491167.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(57102..57785)
                     /locus_tag="ACG8ZM_000170"
     CDS             complement(57102..57785)
                     /locus_tag="ACG8ZM_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0491168.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            58005..60191
                     /locus_tag="ACG8ZM_000171"
     CDS             58005..60191
                     /locus_tag="ACG8ZM_000171"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0491169.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        19227 a        10757 c        12213 g        18113 t
ORIGIN      
        1 tttttcatgg cagggccttt tcatttgaaa taggagatcg acgatgaata aaaatatttc
       61 tttaaaagaa agtatcgctg tattgatcgt gttgttggca ctcctaggag tactgatcat
      121 cggattccag ctctcgccac atatcccgat ccttgcagct ttcatgtgtt tgttgtttta
      181 tggcagattt aaaaagttca catgggatga gatccatgac gggatcatca aaggaatcac
      241 tccagggatc attccaatcc tgatttttct tttgattggt gtcttagttg cttcatggat
      301 tttatctgga accatcccta cgattatggt ttatggattt aaacttgttt ctgtgcgttt
      361 ctttttaccg acagcatttc ttgtttgtgc aattgtcgga gtgactgtcg gcagttcatt
      421 tacaacgatt tcgacgattg gaattgcttt tttggggatc ggtcatttac ttggattcaa
      481 tgatgcaatg actacaggcg cagtcgtttc cggtgccttt ttagggaata actgctcgcc
      541 tttatcagat acgaccaact tggcagcggg tattggagga gttaatctat tcgaacatat
      601 cgctggtatg cgttatacgg atttcccagc attcgtcctt tcgttgattt tttatacgat
      661 attaggacat aacagtcatt cagccgatct agctagtatc aatgaaatga tccaaaacat
      721 gaaagcgagc ttttggatct ctccttggac attgattcca ctagttattt tatttggggg
      781 ggcaatcaaa aaaattccgg ctatccctac attactagcg ggatcgacag ctgctctcgt
      841 tctttctttt atccatgaga aaagtttgac ggtcggaaaa gcagcagaca tcttgatgaa
      901 tggttatgtc gcaaatacac ctgatccaaa gctagatgaa ctgctttctc gaggcgggat
      961 catgagtatg ttaggttctg ctagtttgat tttacttgca ttggcattag gtgggttact
     1021 gatcaaatat ctgatcgttg ataccatcgt tcaagaatta aaagaaaaga tggaccgacc
     1081 ttctcgttta gtcgggttta ctgccttgag ctgtatcgga atcaatctga tcgttggaga
     1141 acagtatctg tccatcattc ttccaggaga aacattcaag cgttcttttg atgacgttgg
     1201 cttatcaaaa acctatttga cacgaacact agcggattca ggtgcgaccg tcaattctct
     1261 cgtaccgtgg ggagtcagcg gcacattcat tatgggaacg atgaaagtta gcgcgttgca
     1321 ttacttgcca ttcgcctttt tctcgattct tgcacctgtc tttacaatat taggcggttt
     1381 tttattgaat cgtcgacgtg taaaaaattg aaggaaggaa aaaagaggct gggacagaag
     1441 cgtttaactc caagaaataa gaagaaactc aagaaaattg ctttttaaat ttttgtgaat
     1501 ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg
     1561 agacaaaagt gttctttact tttgtcccac gctctttttt acagataaac aatgtgttag
     1621 aagtagtatc ttcttttaca taaaaagctg accataggat aaatggtcag cgtatttagt
     1681 gagtcagcta tttagtatgg gttttctaaa ggaacgactg tctctctttt gatcaagcga
     1741 tgagggatga ccaaacgtac gccactgctc aattcttcat ggatcaattc gcgcagcttt
     1801 tgcatagcga cacgtcctaa ttcggagata tcgatatcga tacttgtaag gtaagggtgt
     1861 gttaatgttg agaagatcga gttgttgaag ctaatgacgg agagtgtctc tggaatgaca
     1921 tagccataca tttgcgccaa ctgcatcgtg cgaagggcga agatatcgtc aatgaccacc
     1981 aatgctgtgg cttcgttttc tttcaacgtt ttctcaaagg acagataatc ctccggatgc
     2041 tctagtacta cactggataa cgcaggaaga ccagccatca tcattgcttt ttgataaccg
     2101 aaatatcgtt cgaaatacaa actttcatga gtcgtgtttg tcacaaacag gatattctta
     2161 tgtccattct ggatcaaaaa ttctgttgct tgtttaccta gaagctggtt atcattatct
     2221 acataaacga tttcgttctc atgatgataa ggctgtccga tcaaagtgaa agggatgtgg
     2281 ttttggtata aataatcaat gacagtatct tgactatctg aatacactag aataaaacca
     2341 tctacttgtt tttgtaaatg catacgctgg acgttctcta acagcaattc aaaattttta
     2401 gcagtagcga ttgctgtagt catatcataa ttccgcgctt cttcgttgat tgcttccatg
     2461 atctcaagat aaaatgggtt accgatccgc tcttttgaat caagaggggg taagattacg
     2521 ccgatggcac tggaaatgcg ttttcctaga ttgcgggcag tcatgttagg aacataacct
     2581 agttcatcca taatttttcg gactttcttc tttgttgtct cggaaatact aggatgatcg
     2641 ttgatgacac gagagacggt cgaggtggca acaccggctt ttttcgccac atcttttacg
     2701 gtaatagcca tgataaaaac acctttctct tgttattgtg cagagaaccg ataaatcgtc
     2761 tgactgatcc gttcttgacc aggatggagg ttgatcgatc caaatttggg gaaatgaacg
     2821 atatcaggga tttcttgcgg ttcgattgct aatccgtagt ttgaatgcat ctgttttcca
     2881 ttgacagaga aatcagcttc gaatccagtc gtagaaaata ataccatact tttattagaa
     2941 gttgctatag tcatttggcg accagaatgc ttctcgaata attgtaagga agttttggaa
     3001 agcctaggat gatgcaaaag aaatacatca tctagtccat ctggataaca tgcaaggatc
     3061 tcttgcagtg ttggggattt tctaaagtcg attggcagtt tttctttttt tgtccaattt
     3121 ccagtaggca gtttttcttg atctaattca agcacaccat ctaaaaagac cgttaactga
     3181 tgttcataga tgtctcttgt tccatttcct gaaaggttga agtaggcatg gttggtcgga
     3241 ttgcagatgg tttcaacaag gctactagca gaggttgtca tctctaattc gttttttgtt
     3301 aaacggtaag tgatcgtagc agtgattggt cctggatagg tagaaaattc atcttttaag
     3361 taaaagacca cacctattga tgatggagtt ttgaaaactt ctacttccca aaattggaaa
     3421 gaccagcctt tcgtgccacc gtggatatgg tgtgatccgc agtttttctc caatctatgc
     3481 tcaccccaag aagcatcccg gatccttccg gctacaggtc cgacagttgc tccaaaaaag
     3541 cttttgtctg ttagaacatc ttcgaaacga tcgtaagaca ataaaacatt ttctgatttt
     3601 ccttctttgt ctggtgcgaa gatttggtgc aggcgggcac cgtaattgag aagaacgact
     3661 tctaagtagt cgttcttcaa tgtgattttc tttatttcag tctctggaac tggtacgacg
     3721 gagatctctg gattatccat ggtcatgcca cttttctttc aagtaatcga tcgtaagaaa
     3781 agtcgtatca gggacaagcg ggatctcctc tccaaggtct tcagcacgtc ccacgccaat
     3841 tggttgagcg ccacttgaga ggatggcttg gataccggct tgcgcgtctt cgatcccgat
     3901 acattcttct gcttttagtc cgacagcttt tgctgcaagg aggaagatat ctggcgcagg
     3961 ttttccgttt gttgcttctg ccgggtcggc aatagcatca aagtaaggtg taagacccat
     4021 tttttcaagt aggaatggcc cgtttttact tgcagaagca agagcaatct tgattttttc
     4081 ttcttttaaa gaatccagta aaggcacgac cccggggaag acatcttttg gttcgatcgt
     4141 ctggatcatc tctaaataat aatcgttttt tcttttcgct aattctgcaa attcttcctt
     4201 tgaaaaagag tgttcttttc cgccataagc taaaagcaaa gagagtgagt cttctcggct
     4261 gacgcctttt agctgttcgt tgaatgtgtg gtcgatcgta atgccgattt cctgaccaag
     4321 ctttcgccaa gcacgataat ggaattcagc agtatcggta atgacaccgt ctaaatcaaa
     4381 caatacccct ttaaacattt gcttcctcct ttcttagcgt tatttctaga cgatccgcta
     4441 aagaataaga ctgttcatag acgtaaattt ctagaggaga accttctaat aactcgaaag
     4501 taacttgttc atctgtgact gagatgaaca ataaacgtcc gcgataattg atatggaaag
     4561 aataagcttt ccaagttttt ggcagaaatg gtgcgaatct caactgatca tgggcagttt
     4621 tcatttgagc aaatccttgg acaatcgaca accagctacc agtcatggaa gtgatgtgca
     4681 agccgtcttc tgtgtcgtta ttgtaattat ccaaatccaa gcgagctgtt cgctggtaca
     4741 tttctacagc tttgctttcc attcctaatt ctgctgctaa aatcgaatgg atacttggcg
     4801 aaagggaaga ctcatggaca gtcattggtt catagaaatc aaagtttttt tgcttttctt
     4861 cttttgtaaa agtatcattg aagaaataaa tcccctgtag tacatctgcc tgcttgatga
     4921 agcaagatct aaggattttg tcccaagacc aatgctgatt cagaggaaga tcgcttgtgg
     4981 gaatatcgga tacaggcatg agatctttat ctaagaatgt gtcatgctgg acaaagattc
     5041 ctaattcttc gtcaactggg aagtacatgt tctctgcgat atctttccat ttggctagtt
     5101 cttcttcgct gatagtgata gcagcttctt tttgataagt taaataattt tctcgcgtgt
     5161 aacgcaagac ccaagctgcg atcgtatttg tataccaatt gttattgata ttgttctcat
     5221 attcgtttgg gccggtcaca ccgtggatca tgtatttatt tgctcgtttt gaaaaatgga
     5281 cacggtctgc ccagaagcgt gcgatttctg ctaaaacttc cagtccttca tttttcaaat
     5341 attccgtatc gccggtgtaa ttcgtataat tgtagattgc ataggcaatc gcgccatttc
     5401 ggtgaatctc ctcgaatgtg atttcccact cgttatggca ttcgacacca gtgaaggtga
     5461 ccataggata aagcgcgcct tttaatcctt gttgctgagc gttgtggatt gcttgcggca
     5521 gttgattatg ccgatatttc agcagattct tagttacttc aggtttggct aaagcaagat
     5581 aaagagggac agcatacgct tctgtatccc agtaagtggc tccgccgtat ttttctcctg
     5641 tgaatccttt ggggccgata ttcaatcgtt catcttcgcc gtagtaagta gagaatagtt
     5701 ggaacaaatt gaatcgaata ccttgttgtg cttcttcgtc tccttcgatg accacatcgg
     5761 caagcttcca tcgttctgcc caagccgctg tttgctcttc ttttgcttgg atataattgt
     5821 ttgtctggtt cgttaattct gagacagctt tttctgcttg ctgcgcttct ggaatatctc
     5881 tactagtaag aacgataatt tctttttcca ctgaccatgt ctcaccttga gataattctg
     5941 cagacaagca accatgtaat gctaaagtat cttcgtgata gtcagggatg actgattttc
     6001 cattaacgaa atggcgcatt gctgcagtaa cagtaaattg ttcaatccca aagttgtttg
     6061 gaatagtttt agtcgtcaaa tagccgattt cttctatagt tccacgatca atctcattcc
     6121 aaaaatgttc atcatagttg ctgtcttcgt tttgaacatt tccatccaat tttgatacga
     6181 gtttgactgt tccgctacct tcaagcattt ctatagttaa atagatataa gctgattctt
     6241 ttttgacaat gctcagaaaa cgttcgaagg aaatgcgtac tttattagta gaagtagtga
     6301 ttgtgaatgt acgagataat acaccatttt tcatatctaa ttcccagtaa aagtcttctg
     6361 gttccaaaga agccaagtcg atcagctgat cgttgatcga aatatctatc gcgataaagt
     6421 tcaaggcgtt gatcactttt ccaaagtatt ctggataacc atttttccac cagccgacac
     6481 gtgtcttatc gggataccag acacccgcca aataagtacc ttgatggtgg tcccctgagt
     6541 aagtttcttc aaagttcccg cgcatcccca tataaccgtt tccaatactt gttaaagatt
     6601 cttgcagtcg aagatttttt ttatctaatt gtgtcgtgct gattttccat ggatcaatag
     6661 caaatagtcg tttgatctgt ttcatttttc tcctcctaat gagctgtttt atacgttcat
     6721 aataaacgca accgattgca caagtcaata gacaaaagaa aatatttcat agaaaaaagc
     6781 ttaaaaaaat aaatttttag gagaaaatta tagaagggaa cacacgtatt ataggtttta
     6841 tagaatggta tagacagaaa aataaaaaat aaagcattta caaaaaagaa aacgcacgct
     6901 ataatacgtt taacgaaacc gattgcgtaa aggagctaaa cggaatgaaa aagatattta
     6961 gttttgagtt ttggcaaaaa tttggaaagg ctttaatggt tgtggtagca gttatgccgg
     7021 cagctgggtt gatgatcagt atagggaaat caattccctt gatcaatcct gattggacgc
     7081 cgcttgtcac aacgggcgga gtaattgaaa atattggttg ggcaattatt ggtaatcttc
     7141 atattttatt tgctttagca ataggaggaa gttgggcaaa agaaagagca ggcggagcat
     7201 ttgcagctgg gatctcattt attttaatca atcggatcac tggggctatc tttggtgtca
     7261 caagtgacat gcttgcgaat gaagatgcat ttacccacac tctgttcggt acgaagatca
     7321 tggttaaagg attttttaca agtgtattgg aagcaccagc tttgaatatg ggagtattcg
     7381 tagggatcat cgctggattt gtcggggcaa tggcttataa taagtactat aattatagaa
     7441 aattgccgga tgctttgtca tttttcaatg gaaagcgctt tgttccgttt gttgttatct
     7501 tatggtcaac aatcgtggca ttgattcttg ctgtggtgtg gccaaacgta caggctggaa
     7561 tcaataattt tggtttatgg attgcagaat cacaagaaag cgcaccgatt ctagcaccgt
     7621 ttttatttgg aacattagaa cgtttactat tgccatttgg attgcatcat atgctgacga
     7681 ttcctatcaa ctatacacag cttggcggat cgtatgagat tttatcaggc gcacaggcgg
     7741 ggacacaagt atttgggcaa gatccgttat ggttggcttg ggccactgat ttagtgaatc
     7801 ttaaaggtgc aggggatatg tcgcaatatg agtttgtgtt gcataactgg acgccggctc
     7861 gtttcaaagt aggacaaatg attggttctt caggtatctt gatgggaatg gcttttgcga
     7921 tgtatcgtaa tgtcgatcct gataagaaag caagatataa atcaatgtat ttttcagcag
     7981 cgttggctgt ctttctaaca ggtgtaacag aaccattgga gttcatgttt atgtttgctg
     8041 ctgtgccatt atatgtcatt tattcagtga tccaaggggc tgcatttgcg atggcagata
     8101 tcttgccatt gcgggtccat tcatttggga atatcgaatt gctgactcgg acaccgttag
     8161 ctatcaaagc gggtctaggt ggagatctta tcaacttcgt gttgtgtgtg atcgcttttg
     8221 gtgttgttac gtatttctta gcaaatttct tgatcaaaaa attcaacttt gctacaccgg
     8281 gacgtaacgg aaattacgac aatgacagtg aagaaaccgt tgtatctaac agcggaacag
     8341 gaacagcaga tcagcaagtg gtccagatca ttcatttgct gggaggaaaa gaaaatatca
     8401 aagatgtcga tgcttgtatg actcggttac gcgtaagtgt aaatgatcga gaaaaagtag
     8461 ggactgaaga agcctggaaa cgagcagggg cgatgggatt gatcgtcaaa gacaatggag
     8521 tccaagcagt ttatggacca aaagcagatg tcttgaaatc agatatcgaa gatctgctgc
     8581 aatcaggagc agagattcca gagccagaaa tggtttctac aggaaatgga caagcaggta
     8641 gtaagcaata cttaggtata gaacaagaac tagtttccgc agcttctgga gaagtgattc
     8701 ctttatctga agtaaaagat ccggtattct cacaaaaaat gatgggtgac ggctttgctg
     8761 ttattccaac atcaagagaa gttgttgcgc cgatcgctgg aaaagtgaca agtattttcc
     8821 ctagcaaaca tgcaattggg ctggaaacga aagatggtat cgaagttttg atccatatgg
     8881 gaatcgatac agtccaaatg aatcagccag cttttgagat tgctgtcaaa gaaggccaag
     8941 aagtaggaac aggaacgaaa ttagctgaga tggatcttga tgtgatccaa aacgaagaaa
     9001 aagatccaac gatcatgatc gtatttacaa acgataaagt agaagaagta gtgatcaagc
     9061 aattaggtac agtgacagct ggtaaagtga tcggctccat taaaatgtaa ggagaggttg
     9121 tgaaaaagct gtcctgcatc aagtcataag aacagccaaa caaaaatagc tcctcctatt
     9181 tttcgtttgg ttgggcttat taccgagaag cagcagtttg aatactgttt agtggagagg
     9241 tcgtgaaaaa gctgccctgc accaagtgat aaaaacagcc aaacaaaaaa tagctcctgg
     9301 tccattgagc ttattactga ggagttagca ggagaacact atttgctgga gaggttgtga
     9361 caaaaatttt gtcacaaccc ttttgtttcg aaatgaagac aaggagaaaa ccaatgaaat
     9421 tcatgacact caacacgcat agctggcttg agccagaacc agagaaaaaa ttgcaagaat
     9481 tagcagataa gatcctgtta gaagattatg aagtgattgc tttacaagaa atcaaccaat
     9541 tactggaatc tgaagaagta gaacctgcga agctaatgaa gttctgtccg gtaaagaatc
     9601 aagtacctat ccgtaaagat aattttgcct accgtttagt tcagctttta aaggaacacg
     9661 gaaaagagta tttttggagt tgggaaatga gccacatcgg ttatgataaa tatgaagaag
     9721 gaaatgcact gcttacgaaa aaaacattgg aaagtcaggt ccttaccgtc tcccaatttc
     9781 aaaagaaaga agattatcag acacgcaaga tcttgattgg gaaaacaaag atagatgatc
     9841 aggacatttt tgtctcgagc tgccactttt cttggtggac agataaaaaa agtggctttt
     9901 attttgagtg gaaaaacttg gaaaactact ttttggaaac tcgcgtacca ctttttttcc
     9961 taggtgattt caataatcca gtagactctc aaggttatta tactgtaaga gaaagttgcc
    10021 ttcttcttca agattcgtat gttgtcgcga atgaaaaagg aaaagcagct actgtggaaa
    10081 agaaaataga tggttgggaa caaaatacag aaaaattgcg tatagatttt attttcgtcc
    10141 ctgaaggaat gcaggttaaa aaatatcaac ggatttttga tgggatcgat agtccaatca
    10201 tcagtgatca ctacggggtg gaaatcgaat tagatgtgaa cgaataaaaa atataacata
    10261 taacataaaa aaactgatta aaataaaact ctccaaacga ttataccgtt tggagagttt
    10321 tttgatcgga tgtatttatc ccttttgcct aagcacgtaa aagcttgtat ctatgataaa
    10381 taaataaaca tagcaaggta aaccagccaa tcaaagtaag agaaagaatg aaatagaaat
    10441 agattggcgt ttgataatga agcgtgagct cgttgattcc tttttgctgc ttcacgattg
    10501 gggtcccgat atctgttact tcataatcag tcaatgtttt tccattttgc tgtagcacag
    10561 tccgatcgta gacgataaca gggatatgaa cagttttgtc cccagtgttt ttccacttga
    10621 ttgtcaaaag accgcctgga tgtgttttta taaaagttgt ttcgttttct agtatttctt
    10681 cgttataccg gtcgtattta ttgtcttttg tctccttata gattggcaga tagtcaggtg
    10741 tggttttttg gaaaacgaac aagctggtag acatatcttt gctgaagaat gttttccttg
    10801 cttcttccgg cgtatcgaat agataagtat gtcgactgac aaatgtttca ttttcccact
    10861 tttccagatg ctggtataag ttctggatcg tctgtgctga aaaaataaga atgccaaata
    10921 tacttaaaag acggaaccac ttttgttcag accactggtt cagtgaaagg gcaaggtaaa
    10981 gtagaagcaa tagcgtaaat ggcacgaaaa atcgaaatgg aaactggatc aaactaataa
    11041 aaggcacatt tttttcgatc aatgtcgtcc atggaatcaa gctagtagaa agaaaaaaga
    11101 aaacaagact tgtgtacaac acgatttttt ggaataaggt gttcttttgt tgaaagaaaa
    11161 taaaaagcaa aaaacctgat aatatcaata taggaaaggc cttagggtaa tggagccaat
    11221 aactgccatc cagatctatc gccttttgac tcatttctct gttaacaaag ggaggaagaa
    11281 tcttattccc actgtaaaca atagctaatc ctgcccaaat atttgctgtc agaacaaaat
    11341 aaagcaagac cgacttaatc agttgaagca gtgcttttcc tttttcagtc tggttaaaaa
    11401 agtaataaag ataaaaaggg atatacatca aagctaacat gatcgaagac agtaaatgga
    11461 tctgtgtcat aagtcccact gagacagcga ctttgataac agggaacttt ttatgaagga
    11521 cgaaatcagc aatagggatc atacataaag gataaaaaca agtcccccag ctagtgaatc
    11581 cttggttgat agaccagtat tgaatagaat acgtactcat gaataaaacg gctactggta
    11641 cacttaccca atttcgaaca ttgacatatc tcagtaaaat cagcatagaa aatcctgaaa
    11701 taagataaag aatgaaattt gaaagaatct gataatgaaa ccagctacct gcaagcaaca
    11761 cgatcaatcc ttgtaaataa gcgaaaaatg ggccgtaaac ggcattcacg atcctgccac
    11821 tttgctggaa tccataaagg gaaggaaaat aatggaaatc cccatattta atttgttgcg
    11881 cggcatcata gaaccgattg taatggaatg gggcatcagc acccaaaatt agtttatgtg
    11941 tgaataaatg aggcatgaga aatgccaaag caatgaaaaa aattgctaaa aatgggccat
    12001 attttttcaa ttgtcgaact cctttgcaaa ctttagataa attcatcata tatcctatgc
    12061 gagaggtagg caagaaatta gaaaattcat gaaaatattt atgaatttta caagaaaaac
    12121 ataagtgaaa gttctaagga gagaggatgc gacataagtt ttgttgcatc tccttgtttt
    12181 gaaacagaat ttttcttaat actcgaaaca aagtagcttt ttcatgacct ctcggtaaac
    12241 ggtgttcaat cagctgcttc tcggtaataa gctcactagc ctagaaaata tgagaagcta
    12301 ttttctgact agcgctctta ttactcaaag cataacgcag ttttcacaac ctcttcctta
    12361 aacggtgttc aaaagcttgc atctgcggta ataagcccaa cccaacaaaa aatatgagta
    12421 gctatttttt tgggctgtac ttattacttg atgcaggaca gctttttcac aacctcatcg
    12481 gctttcattt tctatttttt tcctgaaccc atataataaa agaaagaaca aaaaaacttg
    12541 caagggagga cacatgtgaa gcagagtaag caaaaacagt ctcgtcgact tttgtttcgt
    12601 ttgtttttat taatatgtat cgtcgtaggg ttactgatcg ctttatatcc attttatgtg
    12661 gattctttaa atagttttat tgatcaaaaa cgtatcgaac aagtacaaaa acggaccgca
    12721 gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa accaacgatt aactgaccaa
    12781 ggattcaatc ctggtactga tcctttcaat gaaaagaaca gaaatgaaag tacaacatcc
    12841 agccaattag aggaatggct gatcggaagt atcaatatac ctaagatcca attaaatatt
    12901 tcactttatg atcggctcaa tggtaggatc cttgaaaatg gagcaggcgt cttgcaagga
    12961 acctctttcc cgcttggtgg aaattcgacg cattcagtga tctctgctca cagcggttta
    13021 cctaatcgac ggttatttac cgaattagat cgtttagaaa acggagacac gtttattttg
    13081 actgttttgg gggagaaatt ggcttatcaa gttaaaaata tacaagtggt actgccagat
    13141 gacacgtctg ttctaacgat ttcagaagga gaagacttag ttaccttgct tacatgtacg
    13201 ccgtatatga tcaacacgca tcgtttgcta gtgacaggac atcggattcc ttataacgaa
    13261 tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa ccatgcggca atggtttatt
    13321 cttgcaggaa cgataatagc tgtagtcata ctgttagtgt ttatcggacg actaatctat
    13381 cagtatagat taagtaaaaa agtgcttgat ttttcattta ttattagtga ttcgtcaggg
    13441 aaaccggtaa atggtagttc attcattttg aaacataaga aaaaaacact tactagaaat
    13501 ggtgtgccgt tttcagtaca aagcgaccat catgggaaag tcaagctgga tcaattgcct
    13561 ggtggaactt atcgaatcgt ttcagctgat gatcctaaag ttgctgtttc ttttggtatt
    13621 agaaaagtaa aacaagagaa gatatacttt tttgaggggc gaaaacttgt aaaagagctt
    13681 caaaaaaatg gtttttattt taaaatcaat gaataagtaa ggagagaaaa aatgatccgc
    13741 acaacaacaa aattgtctgc caaagaactg gaatctgttc tatcgatttg gctgcatgct
    13801 aacttggatt ctcatgaatt cattccatca acttattggg agaatcaagt agaatctgtt
    13861 aggaaagctt taccagatgc agtgatttac ttagcagaaa tagatcaaga aattattggt
    13921 tttgctgggg tgattgatca gtttatcgca ggaatctttg tgaaaaggga ataccggcaa
    13981 caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa ccaataaaac actgactttg
    14041 acagtctatc aaaaaaatca atcagctatt catttctatc tttcacaagg ttttacaatt
    14101 caaacacagc aaaaagatga agcagtaaat gaggaggaac ttgtgatgaa atggaatcaa
    14161 aacgctgatt tttgatgaac agaataacga aataatgctc ccagcctaat tttttgctgg
    14221 gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt tgttacaatc
    14281 tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat
    14341 gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    14401 tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag cctagaaaat
    14461 atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct gatttcacaa
    14521 cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa
    14581 catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc tgatttcaca
    14641 acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta ttttccgcta
    14701 cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc attgtctata
    14761 cctgcatcac ccatataagt gatcgtagca aattcaggaa caagtaattt tggataagtt
    14821 tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa agtaacagtg
    14881 atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag tttcttttca
    14941 tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt gattttgaat
    15001 acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg gcctttgacc
    15061 attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca ttcatattcc
    15121 caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa ttctttaaat
    15181 tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac atgttttcga
    15241 ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac tgatggcaag
    15301 aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga tgacaggata
    15361 tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa aacatagtca
    15421 gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc aagtagagat
    15481 cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga aggaacaatt
    15541 ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg aatttgttct
    15601 gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta ttcagcagca
    15661 gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaaa cgatctttcc
    15721 tactgaaaaa gaaaaacatg aaaattacgg acactacctg attttagcgc atgcgatcaa
    15781 tcgggaaaca gaatatgcaa attggctaac agatgtatta gaagaaagta accgaaaaaa
    15841 ataaaaaaat atccgaacag tgatggagaa ttcctttcta tatgaaagaa acgcagatca
    15901 ccgttcggat attcttatgt tcaaacaggc cttttttcaa gtatttatgt agcatctact
    15961 tattaaaaag cttggtactt aggagattag tggcgtttag agttctttta acaactgttc
    16021 gtagtcagct ttttcatcga caggaaggag cttgtaagtt aattcgtctg tctcgttgtc
    16081 atgaatgatg tacatcttag cattctcttc tttgaagtta ccgatttgta aaatcgtata
    16141 gcgttcgtcg atcaattcga tatctttgat tgcttttgaa aaaactttac ggacatggtg
    16201 cattttcttt tggcggattt ctgccttttc tgcagctttt gacaaagtaa atccgtcatc
    16261 taaatatttt ttgatcagtt ctacttttat gactgtaggt aaacgatatt ttctaggaca
    16321 attcgcatca gcttcgatcg aacgaatgaa tcccttttgt tcccaatacc ttaattgacg
    16381 tggagacaca cctacgatat cacttaattc actgatgcca acaaccaatt gatcattttc
    16441 caacaaacgt ttaagggaag ccccaaccat ttttgtcact ccttttgtcc tcttgcttcc
    16501 cttaattata tccgtattaa cttatgtgtc aagtaggttg acaaatagcg aatttaggac
    16561 taaagtattc tagtagtttc agaaaaaatt cagaaagaag gagaaatatg agtaacaacc
    16621 aacccgtaga tatttacgga aaaccatata atcggacact tttagttgtt gtcttattaa
    16681 ttggtacatt ttgtaccgtt ttgaatcaga ccttattgac gacagctttt cctacattga
    16741 tgaaagcttt cgatatttca gcgtctgatg tgcaatggtt aacgactggt ttcttgctag
    16801 tcaacgggat catgatccca ataacagcat ggctgatcaa taaattcagt tcaagaaatt
    16861 tatatctttc tgccatgacg atctttttga tcggaacgat tacttgcttt acagcaccta
    16921 attttagtac attactaatc ggtcgtttga tccaagcttg cggtgtggga gtatcgatgc
    16981 cattattgca aaatatcatg ctatctattt tcccgccaga aaaaagagga tcagcaatgg
    17041 ggatgtcagg gatcgttatt ggacttgctc ctgctttagg ccctacatta tctggttata
    17101 tcattgataa ctaccattgg cgtgacttgt ttggtatggt actgccaatc gttgttcttg
    17161 tcttggtttt agcattcttc ctaatgaaaa gtgttattcc attgtcaaat cctagcattg
    17221 atattttatc cgctatttta tcaacgattg gattcggtag tttactttac ggattttcta
    17281 gtgtaggaga tgacggctgg ggaagcttga aagtaattgg tttcttagtt gttggtgtga
    17341 ttttcatcgc cttgttctca tggcgtcaat tacatttgga acatccattc ttggaacttc
    17401 gtgtattcaa atcatctaca ttcacgattg cagctgtttt agctggtgta acaaatatgg
    17461 cgatggtagg ggcagaaatg gtcgtaccat tgtatattca aaatattcgt ggagaatcag
    17521 cattccattc tggcttgatg ttgttgccgg gtgcattgat catgggggct atgatgcctg
    17581 tgacgggtgc gattttcgat aaatacggcg cgaaacgttt agccatcaca gggatgttca
    17641 ttttgacagc ggcaactgca ccatttgctt tcttgacaaa aacgactcca gttttatata
    17701 tcgtgatcct ttatgcgatc cgtatgtttg ggatctccat ggtcatgatg ccggtaacga
    17761 cttctggtat gaatgcattg ccaaataact tgatcagtca tgggacagcg gtaaataata
    17821 cattccgtca aatcgcaagc tcgattggta cagctatttt gatcagtgtg ttgacgaatg
    17881 tgacaaaagc aaatatccca agcaaatctg tattggataa tacgccatta gcttataaag
    17941 atcaggcaat caatgccaca ctgtcaggtt accatgccgc gtttatcgta gcgatcatct
    18001 ttgggattct tggctttatt attgcctttt tattgaagaa aaaagaacct gttctaatga
    18061 aagcggggga taatgcatga ttattggagt tttgattgtc agcttgcttg cttttgcttt
    18121 aacgaacgtg ttcgctaaaa aaacatggca aaccttttta tctttgattt tcggactaat
    18181 cttcgtcgct tcactaagtt tgatcgtcgc taatttgtcc aatcactttg gaatggaaaa
    18241 agtaacggaa acaaaaacag aaaaaattgt ttccagtgct gattcccaag gagcagatat
    18301 gcttttgtat aaagctttag ggaatggaaa agaaaaagtg tatctttacc gaacaaatga
    18361 aaaacaagaa aaaccaaaag caactggtac ggataacgaa acaaataaag tagaaaagac
    18421 agacggcgat gctgaaaaag tgacgaaaac tacttattgg gaatacaaaa acgacatgta
    18481 taaattctgg ttcaatattg ctgataacaa tcacgaatat gacagccgag tgaatacatt
    18541 caaaatccca gagacatggg tagaattaag tacggaccaa gcagccaagt tagctgaatt
    18601 agtgaagaaa cagcaaagta cgatggaaag tgaagcaaaa gcttatgttc aagacggtat
    18661 ggtaaaagca atgactgaaa atccaaaaat gagcaaagca gagcaagaac aacgtacgaa
    18721 agccttagct gctgaattcc aacaacaagc attcgcaaaa ttagtgaaag aagcaaaagg
    18781 cgaataatat tttgaacacc atgtaggagg ttgtgaaaaa gctgctctgc atcaagcagt
    18841 aagaacaatc agataaaaat agcactccat atttttcatc tgattgggct tagtgccgta
    18901 gatgcaagct tttgaacacc gtttaaggaa gaggttgtga gcctgccgtt taactctgag
    18961 tcacaaggag caatttcaaa aaacagcttc tcatgtttgt tgactattgt gacttgtgac
    19021 cgaggagttg gcaggagaac accgtttatt cgaaaaaagg aactgcgaca agacttttgt
    19081 cacagttcct tctttttttt gctatgctat actagatgta aaaaatagga gaaataaaat
    19141 gagggaaatt tattcttttg aatcaaaaat ctatgcttcc gaagtcggca agggcggggc
    19201 atatattatt tttccgtatg atataagaaa agaattcggt gctggaagag ttaaagtcca
    19261 agcaactttt gacaatgtgc cgtatgaagg cagtatcgtg aatatgggcg tgaaaaatcc
    19321 agatggttca gtctgctaca tccttggact acgaaaagat attcgtaaaa acatcggaaa
    19381 agaaatcggt gatattgtag cagttacagt gaagcaaaaa taaaagaggt tgtgacaaaa
    19441 gacgtgtcac aacctcttga taaacggtgt tcaaaagcca gtttctcggt agtaagtcaa
    19501 atttatgcaa aaatgtggaa acccatgttt acaaaatttt tcttactact cgaaacgaag
    19561 cggctttatc acaacctctt gataaacggc gttcaatcag ctgtccctcg gtattagctc
    19621 aaaaacggca aaatatgaga agctattttt ccattttttt ctctcatact caggtcataa
    19681 cgctgatttc acaacctctt ctttcataat ggtttttcac catctttata ataaactggt
    19741 tatttgatat ggaaatctat tttttccaac caatctaaaa tcttgtaacc taactcttct
    19801 gacatcggaa aagaggcgtt gatttgtgca ttggagatat ggtcaacatg gataccggaa
    19861 gtgatcgtac aacttcccgg aacggcagtt ttgatgatca cagctatttt ttcggctaag
    19921 acatcgtctt tatggaaccg accatcgtgg ctagggaaac gtacagtttg catctctgta
    19981 tcacgagaaa atgtagtgac tgttccgata tgtggtgtat ctccgcctgt taaagtgatc
    20041 aataagtctt taccgattac tttaacagca gcttggatcg tgaaacctag tttggttact
    20101 tcaactatcg tttctttttc catattaatc accctgccaa cgttttgaat gctcatttac
    20161 aatttcaaat tgatccaaaa gtttcattgt atggtgatta acaagatctt ggatcgtttg
    20221 aggctgatta taaaaagcag gaattggtgg tatgatttgc acacctagtc gtgctaattt
    20281 agtcaagttt tccaagtgaa tggcatttaa tggcgtttca cgaggaacaa tgactaactt
    20341 tcgttgctcc ttcattgtta catcagcagc acgtgcaatc aggttgtcgc ctaaaccaac
    20401 agagatacct gcgactgttt tcatacttgc cggtacgatg atcattccat cggtcaaaaa
    20461 tgatccgctg gcgacagcag cacctaaatc tttgtttgaa taatggtaat caaacaggct
    20521 ttcgaattca gttagtgata agtcggattc cagctttaag ttctgttttg cccatggact
    20581 gatgattccg tgtgtttcta tcatcggata ttcttttagt ttcttcacta ggttgatggc
    20641 ataaatggtg cctgaagcgc cacttacacc aatcacgatt tttttcttcc gttgtgccaa
    20701 ttgttccatt ttccgcactc ctttttatga tgtcaaaaca gttgcagatc attcattatt
    20761 tcaaatattt ttcccaatct tctacttctt ggaattttgc acgtacaaaa tgttctttca
    20821 tatcgaaagg aactgtaccg tcaaagatag tttttgcact tatcccacgg aaacgaatag
    20881 agccaggatt atattctggt aattctgatg ggtccaatgg atggttcctc attcctggga
    20941 tcacgatgat atctttatct ccttggaaac gagtgttgaa agtccaaact acatcgttca
    21001 tgtcgaaaat atcaacatct tcatcgacta aaatgactgt cttcaattct ttgaaggctg
    21061 ataaagcaag aatagctgct tgacgctgaa taccttcatc cgcttcgttt tctttacgga
    21121 tttgcatgat ggtcatcaat ttaccaccac ctgcaggagg attgtagaca tttactactt
    21181 ttcctggaat agctttatcg accaaggcaa gaatacttgc ttctgtagga ataccggcca
    21241 tagatacgtg ttcttcactg ggaccaatcg ttgtttgcat gattggattg tcttttcggt
    21301 gagtaactgc tttgactttc actacttgta cagctggatt agcatcccca gtatagccag
    21361 gaaattctgg cattgctttt cctgagttcg tgttgatatc ttcttgcatt gtttcatttg
    21421 gcatgatttc tgcttcaatg atgaattcag cacgagcaat acccacttca tcgactgcta
    21481 taccatcaac gatttgaata ggttcgttac gcaaagctcc tgcacaccat agttcattgt
    21541 agccaagtgg agtggtaggt ggttcgaaag tcgtgccaat ggcaattgct ggatctaaac
    21601 caatattgat agtgataggc attggtttat tcaaggcttc gaattgtttt tggaagtgac
    21661 caatgtggcg tccgccaggc atgatgtaca taccgatcgt atctttatct tctagaacca
    21721 tacgatggat cgttacatcg ctcattgttt tttctggatc tgatcctaag actacaccca
    21781 ttgtgatgta tggaccagca tcttcaggag tatttgtagg tgcaggcaaa atgttgcgaa
    21841 tatcgaagtc tgcatcggta gctagatgaa cgacttcttg tgctggagcg tctgctttgt
    21901 ctactttcac aggtttgatt ggattttcta cagcgtcttt caagaaacga ccaagtgttc
    21961 gataatcatg gtgcaaaatt tcacctacac gtttacgact agccattgtt ccgatgttca
    22021 cacgtgtgcc ggggaaacct ttgatattgt tgaaagtcat agctggtcct tcttgagtcg
    22081 gacgttttac tgtaccgcca gcaccgatat aacggtaaac accagataat tctgcttcag
    22141 gatcgatttc aatatctgtt tcgtgatatt gacctggaag tgtttttagt tcttcgatga
    22201 cttttcttaa atcatacggt tgatctgtca tgagagattc ctccttagtt gtctttacct
    22261 atctatagta aaaggtgaaa aagaggaaaa caattcaact tgttcggtaa agtattctac
    22321 aatggaatgg atgcgttttt ttgataatag aataaactat gatatactta tcactaaaag
    22381 aaagcgcata cttagtgttg gaggaaggaa agtgaaaaaa atggaggagt catttcctaa
    22441 agcagtaaaa gtggaaaata tagcgaatat tttgaaagtt acatttgaaa acggagaagt
    22501 aaaatacgtg aaatctcatt ggacagaaga gataaccgat gccttgcaat ttggaaaaaa
    22561 aggaagagga aaaagaaaaa atcttctcgc actttctcgt aatatgtgga ttgggactga
    22621 agtaacgatt gaagcagatg gaacagtatt cataaatgga aaagacaggt atacgccaga
    22681 agagttgtgg tacaaaggaa agaaaagtat accagaatta taaaaaataa ttaaaaaaaa
    22741 cagcgcgaaa caaggaacat attacttcct cgtttcttgc tgttttctac tatttcagcg
    22801 ctcttatgac tcaaatctaa atgtggttgt cacaacctta ttctgcacgc aaagcgatag
    22861 cggcgtcttt tttcgctgcc attcgtgcag gaagatgacc gccaagcatc gtcaatatag
    22921 tagagacgat cactagaata atcgcatgga caggatttag ttgagcaaca ttttttagat
    22981 ccgtcatgtt gtataagatg ctattgatag ggaacgtagc tagccatgca ataaacacac
    23041 ctaatatccc agaagaaata cctaaaatac acgtttctgc gtcaaatacg cgagtgatgt
    23101 cttttttacg tgcacctaaa gcttttagta caccgatttc ttttgtccgt tcaatcacag
    23161 atgtataagt aatgatactg atcatgatca tacttgttac taaagagatc cctgcaaaag
    23221 caataaggac ataagtgata gcatccatca atccgccagt cagttcagtc atcgttccag
    23281 ctaaatcagt ataaatgatt ttatcttcat ctgatttgcc tttattgtaa tcatctagat
    23341 aatccaagat cttttctttg tcttcaaaat tgttcggata aatcatgata cttgaaggca
    23401 agctgtctcc acctaaataa ctaagcaatg tttgtttggc attttcgtca actttttcag
    23461 tagttaagac atttacacca ctgtctcttt gagctttgac gatttctgat tctttgtttt
    23521 cgttgacgat ttgagtcgtt aattgatcgc tataagcgat tcctggagat aagagattca
    23581 ttgtagaaga agatttcacg cgtaggatcc ccgagatctt caattcatca gaagcattct
    23641 gatacatcgc atcataatct gtattaggga taaaattccc agttggaagt tttgtatagt
    23701 aagtattgtt gtttactaat ttgaatgttg taccaacgat atcatcaaaa tcaagttttt
    23761 cgtcttcttt cacatcaaat ccaagattct tcaatgcgtt gatattagta ttattattgc
    23821 cgtctacgat cagcacgaca tctgttgctg aggctggata acttccggca agtaaagaat
    23881 aattgtcttt taaaaagttt tctttgctag tgtcaagctg tgtagggaaa cttgacactc
    23941 ccacacctgt cattgctgac atcgtactag ataaagaaag agattccgta tcaggatttt
    24001 gattcgagaa gctgacaggc tgtactttgc catttacatc acgtaaaaga ttgattcctg
    24061 tagtacgggt aaatccgata ttattactta agttaggatc gatgtctgtt acgtaatcga
    24121 cgtatgtttg atcaatctta ttcgtgtgct gtgctttatc ttcttcgctg acttttgcag
    24181 taatcgtttt gctgtctgga taatccgctt tgcttgcacc caatccggca tcgtcacgag
    24241 tctggttagt cgttacctta gaaatagtaa tcggaaattt cgccatcgtt tcagcttgcg
    24301 tgttatcgat ttgtttttgg aatccagaag aaagggacag aacgatcgcg attccgatga
    24361 tcccaatact ggaagcaaag gaggtcaaga aagtacgtcc tttttttgtt cggatattgt
    24421 tgaaggaaag ctttaacgct gtccaaaaac tcatctttgt tctgcgtaag ttaaattgat
    24481 catcttttgg tcgttcgatg tgaggatggg aatctgttaa gatcttacca tcagaaaatt
    24541 caatgattcg atctgcgtat tgatgggcca gttcaggatt atgtgtgacc atgatcacta
    24601 atttttcttt cgataactct tggatcaatt ccatgatctg gatacttgtt tctgtatcta
    24661 acgctccggt tggttcatcg cacaaaagga tatctgggtc attggctagt gctcgagcga
    24721 tggccactcg ctgcatttgt ccaccagata actgatttgg ctttttatgc atatgatttg
    24781 tcaagccgac tcggtgcaat gcatcttctg cccgttttcg tttttcgtct ttagaaacac
    24841 cacttaatgt catcccaagt tcaacgtttt caataatgcc tagatgacca atcaagttgt
    24901 aactttgaaa aatgaaaccg atagagttgt ttcggtaggc atcccagtca ctgtccttaa
    24961 agtctttagt agactttccg ttgattacca tatcaccgga atcataatta tctagtccac
    25021 caatcacatt cagcatagtc gttttaccag aaccactagg acctaaaatc gctacgaatt
    25081 ctttttgtct gaaagcgaca gacacaccat ccaatgcttt ggtggtggtt tcgccgactt
    25141 tataatattt ttttatatct tttaattgaa gcataatcac ccttctcccg tattgaaata
    25201 ttcggtatac taataagtat atcagaacaa tgatcgtagt atacgaaata aatgttaact
    25261 gaagatgaac tacaagaaaa aatctagaaa tttttcaaaa aacagaggga gatcaatgaa
    25321 tcgtatttta gtagtagaag atgatccgca attacagttg ctttaccgtt ctgtgttgga
    25381 acgagcgggc ttttcagtca tcaccgcatt gaatggagag gatgcgctga aaaaactaga
    25441 agcgacacgt atagaattaa tcattacaga tatcatgatg ccgaaaatgg atggctatga
    25501 cttgctagac tcattacgta gcaccagatt tgacacacct gttctaatga ttacggcaaa
    25561 ggccgatttt gaggataaga aacgcgggtt caaattagga gcagatgatt atatgacaaa
    25621 acccatcgat gtcaatgaga tgatcctacg tgtggaagca ttgcttcgac gttcaaagat
    25681 caattacagc agtagcttga taatcggaca aacaaaatta gagctagatg cttatacagt
    25741 gttacaagat cagcattcgt tgattctgcc tcaaaaagaa tttctgcttc tgtataaatt
    25801 actgtcttat cccaacaaga tcttcactcg ccaacagttg atggatgata tttggggatt
    25861 agatacgaat acagaagaac gaacgatcga tgtacatatc aaacgtttaa ggacccgttt
    25921 tgaaaagacg gaagattttc aaatcatcac agtacgggga ctaggctata aggcggttat
    25981 ttaaagatga aaaaaaagct attttcacaa ttatggatct attttgctag tattgtcttc
    26041 atttcgatct tagtgacact gctgtgtttt ctcggtttta ttttcttatt gtctcgacat
    26101 tctatctcgc ctgctgaatc ggctggaaaa ccaacgttat acccgttatt cgtctttgcg
    26161 gggttcagta tggtcgtggg tacaggaata tcgatttttg ttgggcggcg tatcctgcat
    26221 ccaatcagtg cgctagggac aaatatgagt ctggtagcaa cgggagattt ttcgattcga
    26281 atggatgaac agcagaaagt agcagaagta cagcaactgt ataaagattt caacgttatg
    26341 gtccaagaac tcaatagtat cgagacactg aggaatgact ttgtgtccag tgtatcccat
    26401 gaattcaaaa caccactagc aacaatccaa ggatatgttc aattgttaca agccccaaat
    26461 ctctctgacg aagaacgcca aatattttta tatcggatta tcgaaagtat cacgcagctt
    26521 tcacaattaa cagagaacat cttaaaattg aacaaactgg aaaaccagcg tatccaactg
    26581 gaaaagaaag agtatcgatt agatgaacaa atcagagaag taatcgtttt ttttcagcct
    26641 aaatgggaga aagaacagct agaattggat atcgaacttg ctgcagtaaa ttacacaggc
    26701 aatgaagagt ttttgtacca agtgtggctg aatatcatgg ataacgccat aaagtacaat
    26761 caaatcaatg gacagattca tatcaaacta tttgagacag ccacagagat tgtactggaa
    26821 gtgaccgatt caggtgtagg aatgaatgaa gagaccagag atcgaatgtt tgaaaaattt
    26881 tatcaaggag atacaagtcg tcaaatttct gggaatgggc taggactttc actggttaaa
    26941 aaaattctag aactgcatga tggacgtatt gattatagca gtatcgaagg agtaggaacg
    27001 acagcaatga tacggttaag taaacaatag gaaaaagaat aaaaccccta aaaattctat
    27061 tgaaggtttt taggggtttt tagtcgtttt tttgtgatta gggaaatgtt cttacggttc
    27121 ttttggcata aaaatcgctg ctttcttatt gaactacgag aaatgttaga ggtgtcattg
    27181 aaacaaggac atgatgcaag aaagagagcg aaagtcatgc gtcgctctct ttttttgggt
    27241 ggatagatgg acagaaaatg tagggcttag ttttcttctt gatttaaata ctcccaaaat
    27301 gtatctgaag ccaaagaatg ctcaccgact ttacgagtaa atgcaagata actctctgtt
    27361 gttggagaaa tagggagaat cacagtattt tcttttaata gattcttgac tgttttttcc
    27421 ataacaatag caatccccat gtcatttgca atcatattga tgatcaagtc gattcttgct
    27481 cctttataag agatttttgg ttcaaatcca gcttcgcgac acagatctag tacaggttga
    27541 tacaagttcg tagtggggcc aaggagcaaa aagttctcct ttttcagttc gcttagatcc
    27601 aatttctttt tactagctaa aggatgattt ttaggtagaa ctgctacaaa atcgtctgtt
    27661 tcagtaggca accactcaat cgaacttggt ggggcctgga aacttctagc aaagtggatc
    27721 gtgtcactat gttcattggc gtcattgtaa gttaattcga cactttccac ttctttcaac
    27781 tggactgtaa attcaggatg gcgtttcaaa aactcggtga ttttggaaaa acctttataa
    27841 ttgatcattg tcggtatcgt taagatattt aatgaaagat tgttgtcttt cgtataggtt
    27901 tcaagcactt cttgcatagc atggtattgt tctacgacat tttttacata tgggagcaat
    27961 aagcttccag cttcggtcaa ttcgattttt ctatgggcgc gttcgaataa tttgacgtct
    28021 aattctgttt ctaacgaaag aatctgttta gaaatatttc cttgggtggt gaattgtcga
    28081 tcagcagttt tggtgaaatt cattgtttcg gctaaatcaa gaaaaacttc gaatttatgg
    28141 atatccatca tattcctcct acttattcca ataaagaata ctttaacaca aaaaatgaat
    28201 tggtgtaatc ttctctgtct gttacaatct ttcttgcagc gaaagcaata attacagaaa
    28261 tttttctgat ttttttcgct tcgtagaaaa agagaggagt tttttgatgc caactggagt
    28321 tttgatcaat gtaggttccg ttttgtttgg cggactaatt ggcggattag taggaaataa
    28381 attaagtgaa cattttaaag cacagctgac gatggttttt ggtgtgtgtt cgatggggat
    28441 gggaatctat tccattgcgc cgatgaaaaa tatgccggct gttatttttg ctttagtgat
    28501 aggaacagcg attggtttga ttgttcattt aggtaatggg ataaataagg gggctgcgct
    28561 gatgcaagta cctatttcta aaatcttccc tagtgaaaag cttggtatga cccacgatga
    28621 atttatcagc acattagtga cggtcattgt tctattttgt gcaagtggta cggggatcta
    28681 tggaagttta gattctggga tgacaggaga cagtacgatt ttgatttcta aatctgtcct
    28741 tgattttttc acagcagcta tttttgcgtg taatttaggg tatgtcgttt ctgtgatagc
    28801 aattccccaa ttcattattt tttatatttt gttcttattg gcaaaattta tttatccatt
    28861 gactacacca gacatgatct tagattttaa agcttgcggc ggattcttga tggtcgctac
    28921 cggttttaga atgatcaacg tgaaaatgtt cccagtagct gacatgatac ctgcaatgat
    28981 cgttatcatg ccactgagct ggatgtggac aaattggatt atgccactgt tataataaaa
    29041 atcaaagaga agccattttt tgctgactaa aaaggatcaa atcttttagt cagttttttt
    29101 gtatttgaca aactgatttt gaaactatat tatatgttgt ataatataga ttgaaataaa
    29161 atattacact tgagaataga aggtgacgta atgcacatcc agattccaac tgttcttctt
    29221 gatggtactg tgctagcagc attaagcaag gaagatatgt atgggtatgc cttaacaaaa
    29281 atcgtacaag aaagtctctc tgtcagtgaa tctacactat atccggtttt gagaagattg
    29341 aaaaaagaag gatatcttga gacctatgat gaaccttttg aaggcagaaa ccgtcgatat
    29401 tactcgttga caccaatggg aagtcgtcat ttagaagaaa ttattgatga atggattgtt
    29461 tttcgagatt ctttagaccg gttattggag gagagcacaa atgaatgaat atttgaatga
    29521 agttgtggat catcttcaag aaattcctga agacgatcgt tttgatttga tccagtatta
    29581 tgaggaatat tttttagatt ctggaaaaac attagatcag atcattgatg aatatggtac
    29641 accaaagcag tttgccttaa aattaaaaat cagttacttt tcagaagaag aacaagcaga
    29701 aaacgagtta gtgaaaaatc caaaaagaca aattcgcctg atttggttaa tcattatcgg
    29761 tttatttgct tctcctgtcc tcattccact agcactgggg tttatattgg ttattggggg
    29821 actattggtt gctttactag cgctcatttt tagtatctat gttttatgta tcagtatctt
    29881 aggtgttggc ctattcatga tcatctcagg aatcagtgtg tttggacaat cgattctgag
    29941 tggattattc tttatcggtg tcggtctgtt cgccacagga agcgcagtat ttatttcgcc
    30001 actgttgctg aaaggaacaa aagggctttt ttatgtcatg atgaactttg ttaaatggat
    30061 gggacgacgt tttattacaa aaagagatct gcattcagcg ggcgtttgaa ggaggacaca
    30121 taaattgaga ttgaaacatt ttttgattac cggtattggt ctgatgctta ctggtggcct
    30181 cctagcaggt gcaacttata cattaggcgc tcaaaagagt ctgacttggg ataatggtcc
    30241 tcggttgatc gatatggtta ctgagacaaa aaaaatcaag gatgcaaaca aaatcgtcat
    30301 ccatgcagaa aatcaaacag tttcggtcat acgcggattg gattttgaga taaaaacgac
    30361 ttatgataag acagaaaagc cagaaataaa ggagaaaaat ggcgtgttga ctgtgaatgc
    30421 agtcaaaaaa tcaacaggtg caatcattag tttagaagac acaagtgcac aaatcactat
    30481 tgttgtccct gcagatcatg aattatctga attgacaatc gaagggaata atagtgcaat
    30541 tgatatcaac gaagtatcaa tcaaaaaagt gaatactaag acacatgatg gctgggtatc
    30601 gttcaatgaa tttacaggag gaacaataac tgcgaaagat gaattaggct cacttcgatt
    30661 agatgaggcc aatgtggata acttgatcgt cacaggtgaa aacagcaact tccatctaga
    30721 agacagtttg atcagcaaag aaggaaaagt catcctaaac ggtggcaatt tatctttgca
    30781 tgagatggaa aacagtggct atactatcca aacgtctggt gaatcttata ttgaaaaaga
    30841 ctaccagcca ataacaaata ctttgttaga acaaggacag aaaaagctaa agatcactgc
    30901 taaagacaca tcgatcaata ttacgacgga tcaagaggac aatgaaacag attgatcctg
    30961 tactcaactg gatattttaa gaaaaaacag caaagatcct tccaatcttt gctgtttttc
    31021 atcgcttaac gcacataact atagtaaaat aagaaagaaa atatctaaaa agaaaacagg
    31081 gtgaaagagt atatgaaggt agaagaaggt caaagagaaa agttaaaaac agaaatggaa
    31141 agattacata cttatatcac tcaactaagc caaaccttct atgatccaga caaagaaaaa
    31201 gtgatggtta attacccgaa caattcagaa ggtcgccaat tagagcaagt gtaccatgaa
    31261 gttttcaaac atttattgac ggtgaagaaa gagctggatt attattcttt accgattatc
    31321 gatacaggaa tattgaagta tgatgggaag aaggaacgat ttatctttaa atctgttcgg
    31381 gaagatttgc ctctttcagc agggatggat ctagaggtct tggtagaaga ctattttaca
    31441 gaagaaaagc actgggttcg aacaaaattg gattatttac cgcaggcagc cggagggaca
    31501 caggcaagcg gttggtacat cactgaagac aaagagttag aactagaagg tgtaatggcg
    31561 cgtattcgaa aaaaaaatta aaaaagtaac attagatatt tattgaagga taaaggagcg
    31621 caggaagtag catgagagaa aaaagagcat tttatatcaa tggttatgca gggattgtcg
    31681 gattggtcgt attagcttta gttggattgt ttttcttcta tttaggtatg tggcaagcga
    31741 aagtttgggc acttttttta agtatttttc tttggttgat cacgttattg ttgttaagtt
    31801 cggcaactgt agtcagtcct aatcaagcaa aagtaatttt gttttttgga caatatttag
    31861 gtacgattcg agaaaatggc tttttcctta cgataccttt ggcacaaaaa atgactgttt
    31921 cgttgaaagt tcggaatttc aatagttctg ttttaaaagt caatgatttg gatggaaacc
    31981 ccatcgaaat ctcagcagta gtagtcttta aagtcgttga tacagcaaag gcattattcg
    32041 atgtagcgta ttatcaagat tttgtagaaa tccaaagtga gacagccatt cggcatatcg
    32101 ctagccaata cccgtatgat acattcaatg agaatgattt gacgcttcga ggaaacacga
    32161 cggcagtgtc tgacgagttg caaaaagagc tgcaagagcg tttagcagta gcaggtgtcg
    32221 aagtaatcga aacacgcttg aaccatttag cctatgcaac agaaatcgct agtgccatgc
    32281 tccaacgtca acaagcaaaa gccattttgt ctgctcgaca aaccattgtt gaaggagctg
    32341 tcacaatcac tcaaatggcc ttagagcaaa tcgaagatgg acaagaaatc aatttcaccg
    32401 atgatcggaa agtccaattg atcaataatt tacttgtctc aatcattaca gacaaaggaa
    32461 cgcaacctgt tatcaataca ggcgacatga aagaccgtaa gtgacatact ttttgtaaga
    32521 taggaagaag gagtttagga cggattattg tcttgaactc tttttttagt tgttttaaag
    32581 aaaatgacaa aattgaaaga agaatgatag ttgattcgaa ggcagaattg ttcgcttttt
    32641 tctataatca gtatataaag taagagagaa ggggaaaaag tgaaaaaact tctgatcggt
    32701 ctacttgttt tcatcgttgt ggtacttgca ggggtgaaaa tcgcagatta cgtagttatg
    32761 ggtggagaag aatactatgt acaaattacg acagatggta caaaaacagt ttcaaaatca
    32821 gactctggac aagaaacagt caactataga tatatcctgc ctggctatac gaaagagggt
    32881 aaggaaaaag aactggactt caagggacaa ttgactcgac cgcttcgtaa aggagcatat
    32941 ttaaaaatca cctggaacaa aaataaaggt gtcacttcgt atgaagaagt cgaaaaaaaa
    33001 gacgtgccga aaacagcact ggaaaaacta attaaagggt agggaaagaa atgaacaaaa
    33061 tagtcgaagt acagaatgta acaaaagtat acggaaaaaa caatgagaaa aagacgcaag
    33121 cattaagcgg catcagcttc gatgtagaaa aaggcgaatt tattgggatc atgggcgctt
    33181 ctggttctgg taagtcgaca cttttgaata ttttatccac attagataaa ccaacagatg
    33241 ggcatatccg aatcaatcaa aaagatgtga ccacattaaa aggaaatcaa ctagcagatt
    33301 ttcgtgcgaa tgagattggc tttattttcc aagatttcaa tttattagaa agcttgacag
    33361 cacaagaaaa tatcgctgtt cctctttctt tacaaggcgt cagaccaaaa gaaatcaagc
    33421 aacgtgtcca aaaaattgca gaacgtttat ccatctctca tattttggaa agctatcctt
    33481 ctgagatttc cggtggacaa aaacaacggg tcgccgccgc acgtgcattg atcacacaac
    33541 caacgattct attaggggat gagcctacag gtgcgctgga ttccaaaagt gcccgggatc
    33601 ttttagatac gatggacgaa ttgaatacaa aagatcatgt atcgattctt cttgtgacgc
    33661 atgatccgtt ttctgcaagt tattgccaac gtattttatt catcaaggat ggcggtatcc
    33721 atcaagaagt gaaacgtgga gatcaaagcc gtgattcctt ctatcgagaa atactatcta
    33781 ttcttggaaa cttggaacag tagggggaga ttgactatgc tttggaaatt atcacttact
    33841 gggatcaaga gccgtttaag agactacttg gtgttattct caggattagt tatggcatct
    33901 gctatttttt acatgtttga atcaatggca agcaacgaag cttttttgaa aagtaatacg
    33961 atcgttgcgg ctacagttat cattttccga ttcggttctg ttttactagg gatcatcaca
    34021 ttcgtttata ttttatatgc aaattccttt ttgatgacga tgcggcaaaa agactatgca
    34081 atgtttatga tgctgggagc aaaaggcaga aaaatcgcac agatgatttt ttctgaaaca
    34141 ttcttagtag ggatcgcagc tactttagtg ggttcagctg tcggtgtagg attgacaagt
    34201 atcgtccacc gattattagt ggatcagtta gatctcaata tttcgcattt tacacctttt
    34261 agtattcacg ggttgttgat cactgttttg ttttttgctg ttttgttttt attagctgct
    34321 gttgtcaatg ctttttcgat tgtcaaaaaa ccgattttag cattgattcg tgcggatcag
    34381 acaccaacaa gaatcaagca gcataaattc ttgtttttcc ttgaagtagt actgggaatt
    34441 gcttttctag gctgcggtta ctatatgatg aaacacgtgg ggacctatca agtctttggt
    34501 ataggggtag ccttagttac gatcgtatta ggaacttatt tcattttcca ttctgtgatc
    34561 attttctttt tatcattact aaagaaatca gaacagattt cattgaaaaa attgaacaat
    34621 ttcacattgt cacagttaaa ctttcgtatt cgtgaatata cacaaatgct ctcaatggtc
    34681 gctatgctgt ttgccttggc actgggcgct ttgacggtag gattaggttt taaaaatgaa
    34741 attgcagact tgacagaaaa caattctgga tatgatctag tgatgaataa tcctcgggca
    34801 gaagatcagc aaaaaatcaa agaactttca ccaacacttt ctgcttctta tactcaaaaa
    34861 gaagatgcgg gaacgattta ctataatgca agtgaatttg cggctcaacc attgatcatg
    34921 ataaaacatg aagatggaac accgccgaag atcacgaaag aaaaagttcc tgctaaaaaa
    34981 atgaatgaac tgactgtaca agaggaactt cgaagctatg aattgccgga acagcaagaa
    35041 aaagaaatca aggtagtcag tcaatcagaa tttgatcaac tgaacttgcc acaatccact
    35101 ttacagctag ttcaggtaaa agacttccaa gcaaacctga aaccaatcga aacacttgtt
    35161 gctcaaaata aaatgaacaa tccatctttg caaaaagtag agtacagcaa ccagaaatat
    35221 gaaatgtatg aagtgtataa tggtctattc tctggttttg aatttatggg attcttccta
    35281 ggtttagctt tcttgaccat gcttgctagc tgtttgatgt tcaagatctt gtcaggaagc
    35341 aagagtgaca ttgctcgtta tgaaatgctt gaaaagatag gaacacgacg cgggttgctt
    35401 aaacaatcga tccgtagaga agtaggcgtg ttattcttag caccaggaat cttaggtgta
    35461 gtccatgtct tattcggatt acaaatgttt ggtctattca tgcaaaatcc atacaaagat
    35521 atttgggttc cgtttacgat attcttcgta ttgtatttca tctactatgt actgacgaca
    35581 tggttatata ctggaatcgt attgaaaaag agattgtgaa aaagctgtcc tgcatcaagt
    35641 aataagaaca gccaagcaaa aatagctcct catatttttc gtttggttgg gcttattacc
    35701 gcagatgcaa gcttttgaac accgtttgtt cggaaaaaga ggttgtgaca atatttttgt
    35761 cacaaccttt ttttcacggt cttttattag aatatagtaa attttttctc gttactcacg
    35821 gaataagctg ttaagaaaac ggtttccatg tatactggaa tcagtgcaaa tgaaagaaaa
    35881 gagggtgcaa ggaacatgga aatcaatgaa caaaattttg gcgaaaacta tcacttgatt
    35941 acgatcacga atcaaaatgg gacatctttg tctgtcagtg atcttggggc acgggttgtt
    36001 cattttcaaa caataatcga taatgaaaaa cgagagttga tcttaggttt tgatgaagca
    36061 aaagaatatc tagaaaaaga cccttatatc ggtgcttcaa tcggacgtat ggctggacgg
    36121 attacgaatg gcacattcaa attagatgat gaaacgttcg aagtaaaaac agacccggac
    36181 actggtcatt gtttacatgg tggtgcacct agctttgaag ccaagcagtg gcattataca
    36241 gtcatcaatg gtgaaaacga agcatcggtc attttctatc agactagccc agatatggaa
    36301 aacggatttc ctggaacttt agatgtagaa gtacggtata cgctgacgaa cgatgataca
    36361 tggcgagtga cggtccaagg gatcagtgac cgcaaaacag tattcaatcc tacaaatcat
    36421 gtctatttca atttaaccgg tgatccagca cagtcaatcg acgaacatat actttggttg
    36481 aatagccgat tatttgctac gctcaataaa gatacaacgc ctacaggaga agtagtttca
    36541 gtaagtggca cagcatttga cttccaacgt cctaaaaaat taggtgaggt ctttgccagt
    36601 gattttcgcc aatcaatgat agtgggcggc atcgatcatc catttttgtt gaaaaaacaa
    36661 tctggtttac taaatgctgc ggaattgaca agcccagatg aaaaagtatc gatccaagta
    36721 aaaacagatg cttcaggaat cgtgattttt acagcaaatt ttggagaaga tggaccggaa
    36781 atgcgcggga ataaattagt aaatcatggc ggtatcacat ttgagactca agaactgcca
    36841 ggagcggagc gacttgaaag ttttggagat gtcactctgg aacctaacca agtacgcgag
    36901 tttgtaacgg aatacaaaat aaatataaag aaatagaata aattaaaaag ggagtgtgac
    36961 agtgtcacat ccccttttta atttattcta tttctttaaa ggcagatcaa gccagaatga
    37021 tttgtctaaa cttaacaagg ttagtacagg aacagttgca gctttaactg tacgagtagt
    37081 tgtttctggt atctgtcttg ccgtaaaatt gttccacaaa tcccagattg aagcgacacg
    37141 gaagatggaa gaagcgttgt tgacgcgttt tatcgttgaa aattctaatc ctgtgattgg
    37201 cacgccatct gttgtccatt tgattggcac atcgaatcgc ttagacatat tgaggatttc
    37261 agcgtttgct tcttcagttg ttgctgcttc ttttttcggt tcatcatatt ttgtcaggtc
    37321 gtacgcttcg atgatcgcgt tcaatttttg tgaatattgg cgatctgtag cataacgtcc
    37381 ttccaaatat ttggttgctt ctttgtagtc ttttgtttcc gacttccaag ttggtttgta
    37441 gaaatcagga ttgccagtca gtccattttt gatcaagtcg gcatagtctt ccaaactttc
    37501 cttgtaagaa ggatatttac ggaattgtgc gcggatttgg tagctttgtc cggaactatc
    37561 ttgttcaagt gtattgaagg tcacgctgct tcctttatat gatcctttga taccaaaaag
    37621 gttatgattt ggttcgcttg ataataagct gtttccagat ccactttcaa ggatagcttg
    37681 agccatcatg actgatgcgt agattccttc tttttgcccg atttcctgtg catcatctgc
    37741 aatctttttc aagaactgat cagttgtttg gttttgactg aaatggaacg agccatctaa
    37801 tccatctaaa atagatgagc cgcctgtcag cgaacctgaa gcagcaggta aacctgctaa
    37861 gtttactcgg acagcaaaat caggcgtgaa atctgaaata gaagttactc gaactttatc
    37921 tcctggttga ggcgcatgga taaattgtcc gttcccaata tagataccta catggtgagt
    37981 aacaccggaa gaaggtcccc agaagacgag gtcgccaggt tgggcctgac ttactggaat
    38041 ctgcgtacct gcatattgct gttcgcctgt ccagccaccg atataatgac cggttgcttt
    38101 catatatacg taataagtca aaccagaaca gtcaaaagta tttggacctt ttgctcccca
    38161 tacatatggt tttccaagat gcttgaatgc ttctcgaacg attgcttgtt ggacattcgt
    38221 tgtatttgta ggcaatacag catcaattgg tgattttaca acaaaatcag aagaagcaga
    38281 agatggattc tctataccgc tttgatttgc tgtatttgga cgagatgaag cattgttcgt
    38341 tccgccattc gatgaaccat tgtttggatg gtttggttct ctctgagtat ttccattgtt
    38401 attcgatcca gatgggtttg tttgattcga agaattcccg ctgttcgaat tattgttatt
    38461 attgtttgaa ttatttgaac cattattatt gttgttatta cccacatctg gtttgtttgg
    38521 cgtttccgga tctggtgtac tagggacttc ttcactagga tcagttggtt tctctgttga
    38581 agtatccggt ccagctttaa attctgcagt aatagataaa ttgttcgtac ctactgtaaa
    38641 ggtctttgca tcataattta cttctttggc accattaaca gcgactgaag caatttgttc
    38701 accttcagct ggcgtaatag tgtaagttac cttcgtttcc gctaataaac cttctaccgt
    38761 tccactttcc gtaatatcca aaggttgctg ttcaggggaa tccgctgtcg aagcagtcag
    38821 ttggatctta ccaatcaaag aaggagcaat tgtaagggaa tatgtctcgg ctggcttttc
    38881 attttctgat ggatcagccg gatcaatttc agttggctcg gtcgatcctg ttgttgagtt
    38941 agttgttcca gttgggtctt ctgtaaaaga atccgttgtt tcactagtag ctgagctgga
    39001 tgtagtcgta tctgttgtga ctgtttgatc agctatttga ttatctacgg caaatacggt
    39061 attggtcgac aaaacagacg gagcaatcag ccaagtagca gctagcaaac gtatcgtttt
    39121 tttatttttc ataataaact agtccttttc ctttcattga ttttctgtct tattcagcat
    39181 tcattagaca ttttttggaa cagataatcg tgtcgaaaag ctttttcttt tcataaatct
    39241 gtctgtgtat tctaaataaa acaatttgta aataagcaac gagcattcat tcgtttttct
    39301 ttgacgttca ttttattgca tagaccacct ttgtttgggg ataaacaagt ggggataaaa
    39361 atggttatgc atatgtaaat tataccaaag tttggaaagt atcacaaaaa tttataaaaa
    39421 taatgatcct aaaaagaaaa agactgaaat atgtgataaa catctcatac ttcagtcttt
    39481 ttcattaact attcataaga cattaggtct tcatcaaacc cgctaacgag agtagtgatc
    39541 attgctacga ctagactaac agccagtcct gctaagtacc agaaaaaagt accggaaatg
    39601 aatgcaggca atgtggtcag actaccaaat acaaaagcga tcgctttcac ttgggcaaat
    39661 cccattacag ctccaccagc agctgcacca atgcaagcag caacaaatgg ttttttcaat
    39721 gggatcgcaa ttccgtaaac agttggttcg gtcactcccg caagtaaaat cgaagcaaaa
    39781 gcagaaccgg agatcgaacg ggtttttgaa cgtttacttc gcagaaatac acctaaagct
    39841 gcaccagcca ttccaaaatt ggctgctcct gtcaacggaa cgattcggtc gaacccataa
    39901 gcagagatat tactgatcat gacagggttt acagcccatt ggataccaaa tattaccaaa
    39961 atattccaac caccagcgat gattgcgcca ctgatcggtc cgttaatatc catcatccaa
    40021 gtaattgcgg cagctaaacc ttcgccaagg taaacacccg caggcccgaa taccattaac
    40081 gtcaaaggaa ccgtgataac taatgaaagt aaaggtacaa atactaattg catattttcg
    40141 cgaatatatt ttttgaggaa tttttcaaca aaagaataaa agaaaatacc taaaatcgcc
    40201 ggcatgacag tagacgtata ctgcatcagt actacaggaa tccccagaaa atcagtagct
    40261 gctttgccac tgtctgaaag taaagaggtc agattcggtt caatcagtgc tgcagcaatg
    40321 acagccgcaa tatatccatt cactttaaac gttttggcag cagaaaaagc aagaaagatt
    40381 ggcagaaaat aaaagacggc attcgacgca gcagacaaaa tatgataagt tccgcttgtt
    40441 tcactaagcc agccaatcgt agtcgctaaa acaaccagac cttttaatat accagaaccg
    40501 gcaaacagcg gaatgatcgg cgtaaagatt tccgatatca aaccaaatag tttttcgatc
    40561 ggatttagct tttctttcgg agctgaatga tcgatttgac gcgtcaattc cttgtaagca
    40621 ggagccaatc cctgatccat gattatttct gcatatctat cgagtaaaat gaaatactta
    40681 atggaaggga tttgctgttt taatttttct gcattggcta ctggctgttt tttcaatttg
    40741 atacgaagcc gagtcgcaca tggaaggaca gaatcaatat tgtcttttcc tcccaactgt
    40801 tcaatcagca tatgaatctg ctcggacagt accatctgtt caggattcat tgtcgtttcc
    40861 attagaagac cactccactc actaatataa tattggcgta aggtgtcatt tcgcctgtac
    40921 gaataaatgc atgacagtta ttcaactctt ttttcatttc ttcatgtggg ataaagctaa
    40981 tcggtgtatc tggtaacaat aatttgattt tttccaatat ttccgggttc attgtcttta
    41041 tttcttccgc caattcaatc gattgtacct caagttcttc taaaacgttt tctaaaacag
    41101 ataaaaaact tggaagaccg tttgagacag ccaaatctat tttttcagta ccaaagggga
    41161 caggcatgcc tgcatccccg atacttaatt tatcaaagtg ccccatttgt gcgattactc
    41221 gtgaaatatc tgaattgatt acttttcctt ttttcatgtt tgtttcttcc tctcattgtt
    41281 aattttagac tgctgattgt tctaaagctt cgcggtaagg gatagaaggt tgcgcaccat
    41341 aagactgcac agcaacagaa gaggctagat ttccataaag gatcgcttct ttcaagttac
    41401 taaggtctgg ctgaagtgtg ctgcttaatg cgccaataaa tgtatcacct gcggctgttg
    41461 tgtcaacggc atccacttta aaagcaggga cgataccgtg ttctttttct gtatgataga
    41521 aagcgccggc actgcctagt gtaatgatta ctgttccaat acctaattca tggagttttt
    41581 cggctgctgc gaccaaactg ttatgatctg tcactctgat tcctgtaatg atttctgttt
    41641 ctgtttcatt tggtataatg atatctgtct tttcaagtag ttcagtagga atcgtttcac
    41701 gagctggtgc aggattcagg atcgttgttt ttcctgcgtc ttttgcaata gaaaaagcta
    41761 ggatcgtcgc atctaagcta ctttcaaact gagcaataat aaagtcactg tttgtaatca
    41821 gttgcttgtt ttttaggact tcttgttcat ggaaagcatt atttgcccct gcatggatca
    41881 agatactatt ttctcctgct gcgtctacca taatcattgc ttgtccggtt gtggcgcctt
    41941 ggatttcagc tatcccagat gtatcgatgt tttcttttgt cagtagatct aataactgtt
    42001 gtccttcgct gtcagcccca acgcctccaa taaaagatgt ccgtgcgcct gaacgtttgg
    42061 ctgccactgc ttggttggca cctttacctc cgccagcgtg aaaaatctca tgcgcatgca
    42121 tcgtttcgcc tggcttgggc atgttggtta aacgaagggt agtgtccata ttaatacttc
    42181 caattactgt aaccgtgttc attttacaaa cctccaacta tctcatttat tttgatggga
    42241 aaattatttt ttaaaacgtt ttagtaaaac gttttaatag ttcgataaaa atatagcaca
    42301 ccttgaaagc tatttcaagg gtgcagttga aaatcttttt tctaatcgta caggtaatct
    42361 tttttcttcc cattcttttt ctgggaattg gatccgatct aacaataact tggcagatgt
    42421 ttgtcccaat tcgaagattg gttgtgcgat cgttgtcagt ttcggcttga tatactcgca
    42481 catatctata ttatcatagc caataatgct gtagtcttca gggatgcttt ttccagcttc
    42541 ctctaatccg cgatataatc caaaagctaa ttcatcattt aaagcaaaca caccagttgc
    42601 agcagaatca agaagttctg ctgtgatctg gtagccacct tgttttgaga actgtgtagg
    42661 taagaggatc agttggtcgt gagggatttg gtaaacatca agagcagatt taaacccctc
    42721 aatccgagcg tggacatttt ccggtggatt ttcaggatag acaagggcga tcgtttgatg
    42781 tcctagacta agcaagtgca tgcccgctag gtaaccaccg cgaaaatcgt ctgttcgtac
    42841 ggcatcacta aatccttcag attttttctg atccaaaaca atgaagggcc ggccttgttt
    42901 tttcaaattt tcgttgattg catctgtaga gacagcagaa gtcgcgataa tgaatccatc
    42961 cacacctcga cgtgtgagct cggctagata ttcgatttct ttttgatgat cagaatcagc
    43021 attacataat atcgtgacga aattctgctt atacaagata tcctcaattc ctcgcatcag
    43081 tgtactgaag aatggattcg tgatatcagg aacgagtaca ccaattgttt tcgtttcctt
    43141 ggtaatcatt tgtctagcaa aatagtcagg ctgatagcct aattcttctt tggcagctaa
    43201 aactttctga acagttttcg gactgaattt ctccgtattt ccatttaata tcaaggagac
    43261 agttgtgatt gaaacgccgg aaagtttagc gacttctttg attgtgatct tttttttcac
    43321 catgttcata ctcctatcta aatcctgtgg ttattttccc atgaaatcaa aaagaagtaa
    43381 atgaaaatca cgtgtgccag tacttatttg ctttgttgga tagcttcgat tatttgtttc
    43441 gttgagcgta ctcgtgcagt tttcggaaat atctttttta tgaacaattg atgggattct
    43501 ttgtcacgat cactgcaagc atcttcagca agcacgatat ggtacccgtg ttgaaaggca
    43561 tctaatgcag tagcatatac accgtttgtt gtcgctacac cgcttaaaat gatagtttcg
    43621 attccacgcc ggcgaagttg aagatcaaga gaagtaccaa aaaaagcact tggattgtat
    43681 tttgtgatag tcaacatatt atctgtatct ttcaaagaat ccttcaaaag taaatcagtg
    43741 aattctggag gtacagaagc agtcattcca cccatatcag tttgttgact caaataaggg
    43801 aaagtggaga tgtctacgtt cactaaaact ttcaaggcag cggtacgttt caatgttctt
    43861 actaacaaat cattgtttgt cagaatagat gctgcagtgt gtggagagag tgtaccgtta
    43921 tttacgatct ctttttgcat gtcgatcgcg acaaaagcag tttttgataa atcaaaaatc
    43981 atattttcct ttaaatcatt catgatgttc cttctttcaa ataacaatgt tttttatgta
    44041 agtaaatcat aaatcgttta tttgtcaact aaattaaagg attcctttct ttagtctaga
    44101 gaaattcagt ggtaaaataa ataagtgtga gaaaacgcat tcgatgcata gatatagcga
    44161 gaaatatcgt gaaaatggag gaatcagagt gaagtatgtc gaagtaaaaa atgaatacaa
    44221 acgatacaaa atgggagaaa cgaccattac agcaaatgac gatatttctt tttcaattga
    44281 aaaaggcgaa ttagtgatta tacttggtcc aagtggagca ggaaagtcaa cgattcttaa
    44341 tattttaggt gggatggata cacctgatga aggtcagatc attattgatg acacagatat
    44401 tgcgcaattt tcagataaac agcttactgc ttaccgacga acagatgtag ggttcgtttt
    44461 ccaattctac aatctcgttc ctaatctaac agcaaaagaa aatgtggaat tggcaacgga
    44521 agtttctcct gatgcgcttg atcctgtaga ggtattgagg caagtgggcc tagctcatcg
    44581 attgaataac tttccgtccc aattatcagg aggcgaacag cagagggttt caattgctcg
    44641 tgcactagct aaaaatccca aacttctttt atgtgatgaa ccgacaggcg cgctggattt
    44701 tgaaacgggc aagcaagtat tgaaattatt gcaaaacgct agcagacaac acggcaatac
    44761 cgttttgatc attacacata attctgcttt agcgcccatc gctgatagag tcattcacat
    44821 taatgatgct aaagtaaggt ctgttgaatt aaatgatcat ccttcatcca ttgatgagat
    44881 tgtatggtag ggggatttta tgaaaacaag aacatatctt aaagcaggtt ttcgcgaaat
    44941 tcggcaatct aaaggacgat tcattgcaat catccttatt gtcatgctag gaacactttt
    45001 atttgtaggt gtcaaaactg ctggacctgt catgcaaaaa acgatggatc actatgttgg
    45061 tacagcagga ctttctgatt tgcaaatcgt ttctactggt ggcttgacac aaaaagatat
    45121 tgctcaagca gaaaagatcc cagatgcaaa agtagaaaca ggaaaacaga tctattattc
    45181 caatactggc aaaaacgaag tgattcaaat attttcttat aacaaaaata acaagcagaa
    45241 caagctacaa ttaactgatg gaaaactccc ggaaaaacca aaccagcttg tattggatga
    45301 aaaagcggaa gatgaagggt ataaaatcgg tgatatttat cagattgatt cagatgaatt
    45361 gaaagaaaaa gagtatacca tcacgggctt tgtccgttct ccattgttca tcaacaattt
    45421 ggagagagga tacgcgaacg tcggaaatgg tagcgtcgat tattttgtct atttacctga
    45481 atccgttttt aaaacagata tttattctgt cttatatctt gattttacaa acgtaaagaa
    45541 tttgaatacg tacagcaaag cttacaaaga taaaatggag caaaatcaag aaaaggcaga
    45601 aaagtattta caagatcgac ccgaagaacg cttagaagag ctgaaaaaat cggccaacga
    45661 agaattagaa ccggcacagc aaaaaatagc ggatggcaaa gcacaaacaa cgcaagcaag
    45721 aacacggtta gaagatgcta aaaaacagtt agcgcaacag tcagcaacta tccagcagct
    45781 tcctgaagcg caacgaacaa tcgctgttca aacattatct caacaagaag ctcaacttgc
    45841 tgaacaagaa aggcaattag ttgagaaaga acgtgaactg gcctctgcag aggaagaatt
    45901 gaatcgaaat gcagaaaaaa ttgaaaattt aactaaacca tcatacctat acaatgaacg
    45961 ttcagaaaat gtcggttttc aagaatttgg tgatctgtct gatcgtatcg ctgcgattgc
    46021 caatgttttt cctgttttct ttttctttat tgccgcattg atcaccttta cgacaatgac
    46081 acgtatggta gaagaaaacc gcagggaaat cgggacatta aaggcgttag gttatacgaa
    46141 agttgaaatc gcaaaaaaat atgcaatcta tgcaagtcta gcctcaggga taggtattgt
    46201 tttaggaacg atattaggaa cgaatctttt gccaaggatc atttttgagc tgtctaacga
    46261 gcggtatgat attgggagtg cgcttatttt ttatgactgg ccgccgattt tacaagcagc
    46321 tgccgcattc ttcattgccg catttggcgc tgcaatgatc gttttattca aagatttaag
    46381 agaacggcct gctgcattgc ttcaaccgaa agcaccgaag ccagggaaac gcatttttct
    46441 tgaatacatc actccgctgt ggtcacgcct tagctttaat caaaaaatca gttaccgaaa
    46501 tctcttccga tataagtcaa gaatgcttat ggcgattatc ggaattgctg gatgtgccgg
    46561 gctgatggtt gcaggcgtgg gattgaaaga ttctctaagt tctgtttcag ccaaacaatt
    46621 tggaccgatc attgactatc aagcaatcgt tacattggat tcatccacgg aagatagtcg
    46681 tcaaaaagca gcggatatgc tgcagaagca agaaaaagta aatgagatcc tagctattca
    46741 tactcaaacc gtagaattga gaaaaaaagg acatgcttcg caaagtctga cagtgatcgt
    46801 accaagtaaa acagctgatt tggatcgttt tatccattta actgatcttg aagagaaccc
    46861 acgctccttg cctaaaacag gaatcgctat tacgcaaaaa gtagcagaac tatttgactt
    46921 atctacagga gatcatattt cgctttatga tgaagatcaa aaagaatgga aagtcgaagt
    46981 aaaagaaatc gtgaaaaact atctaggtag ctttatttat atgtcccctg aatattatgc
    47041 ttcagttact gatcaatcga tgatggataa tgcatttttg ctgcgtacag ataagatgac
    47101 aacaaaagaa gaagaaatgc tttccgagaa actgcttgcc acagacgctg tgacaaatac
    47161 gtctttcatg tccaaacaaa tcgaaacgca agaagaatca atggcgaact tagactcggt
    47221 tgtcttaatc tttgtcgtat tatcaggact gctggctttt atcgtcttgt ataatctaac
    47281 taatatcaat atttccgagc gtgtacgcga attatctaca ataaaagtcc ttggattttt
    47341 tgataaagaa gtcacgatgt acattgttcg agaaaatatc atctttactc ttttaggaat
    47401 catcggagga tttggtgtag gatatatatt gactgacttc atcttgcaac aagcgtctat
    47461 ggagaatgtc atctttccat tagtgatcac atggtgggct tatgtcttat cagcaggatt
    47521 aactatcgta tttacggtta ttgtgatgat tgttacccat ttcaaattaa agcatatcga
    47581 tatgattgat gcgttgaaat cgaatgaata aaaacagagg ttgtgaaatc agcggtttga
    47641 cctgagtatt agagaaacaa acggcaaaat agtttttcat attttgccgt tttttgagct
    47701 aataccgaag gacagctgat tgaacaccat gaatcagagg ttgtaaaaaa gccgtcctgc
    47761 atcgagcaaa aagaatactc aaataaaatt agctcctcat gttttacatt tgagaagact
    47821 gattgccgta gataaaagct tttgaacacc atttatagaa gaaacaagaa tattttgcta
    47881 ttttaaacaa gaggatagac aaatacatgt ctgatctctt gttttttatt gggcaacaga
    47941 cctcttagaa tgtataaaga taaattttac aaacgctttc ggttcagtta taataataaa
    48001 tgataacttg tatatataag ttgtggtagg taataaatca gttgaatgga ggtaaatatc
    48061 catgacattt aaagacaaag tcatttacca aatctatccc aaatcttaca aagacagtaa
    48121 tggtgatgga attggagatt taaaaggaat caaagaaaaa ctcccgtatc tgaatgatct
    48181 aggcatcgac atgatttggc taaaccctat ttatccaagc cctcaaaaag acaacggtta
    48241 tgatatctct gattacacag ccatcgatcc tatctttggt acgatggagg aattcgaaca
    48301 gctaatagcc gaagcaaaga aataccggat agaaatcatg ctcgatatgg tgctgaatca
    48361 cgtttcgaca gaacatgaat ggttcaaaaa agcactagca ggggaccagc attatcagga
    48421 cttcttcatt ttaagagaag agccaacgga ttgggtatca aaatttggtg gaagtgcttg
    48481 gtcgccattt ggagatacgg ggaaatatta tttgcactta tatgatcgga cacaagctga
    48541 cttgaactgg cggaaccaag cagtaagaga ggaactattc aaagttgttc gtttttggat
    48601 ggaaaaaggg gtaaaaggat tccgtttcga tgttatcaat gttatcggta aagacgaaca
    48661 attgaaaaat aataaagaaa atgatggcaa acctgaatat acggacaaac caatcaccca
    48721 tacgtatcta aaagaattga accaagcaac gtttgggacg gattccacta tcatcacagt
    48781 cggtgaaatg agctctacga cgatcgaaaa ttgtatcttg tatacagaac ctagtagaga
    48841 agaattatca atggtcttta atttccatca tttgaaagtc gattatgaaa atgggaataa
    48901 atggtctact cctccatttg attttgcaga attaaagcga ctatttcata catggggaga
    48961 aaagatgagt gagggtaacg gctggaatgc tctatttttt aataatcatg atcagccacg
    49021 tgcgctcaat cgatttgtag ctatcgatca gtatcgaaaa caaggagcag agatgctagc
    49081 aaccatgatc catttgaacc gtgggacgcc ctatatttat atgggagaag aaatcggcat
    49141 gatcgaccca gactattcat cgatttccga ctatattgac gtagaaagtt tgaatgctta
    49201 taagttactc cttgaacaag gattcacaga aaaagaagcg tttcgtcgga tcaaagcaaa
    49261 atctcgcgat aactctcgaa cacccatgca atggtcagac acagaacaag caggatttac
    49321 tacagggaaa ccttggttaa aagttggagg caagctagaa gagattaatg tagaaaaaga
    49381 actatcttct gaagattcta ttttgtccta ttacaagaaa ttgattcggc tgagaaaaac
    49441 atatccaatc gttgcacaag gagattatca tgcttatgaa gcaaatcatc cggaagttta
    49501 tggctttctt cgccaatttg aaggacagca gctgcttgta ttaacaaatt tctatgataa
    49561 agatacaagg atcgccatcc cagaaggatt cacagaagcc aatattctta tcgataatta
    49621 ttccgatact gttcttcatc aaagtctgga gctaaaacca taccaggcaa ttgctttgct
    49681 gaaaaattaa aacaaaaaca caaaacgtcg ttcctacgag ctattctcgt aagatcgacg
    49741 ttttgtgttt tatttaaaat aatcttcttc agaaaatgaa gagttttcat actctccttt
    49801 atcgccataa aatttattgt agcgttgttt gaatttacgg aaaataaaag taacgagtaa
    49861 tttgactaac gtatagatag gaataccaaa aataacgccc acaattccca ttagatcacc
    49921 catgacaagt aaaacgatta agatcgtaat cggatggatc tgtaaacgat cacccattac
    49981 tcgaggaatc accaagtcac cgtggagaag ttgtacggca aaccagacaa tgacaaattt
    50041 gacagccata aaagtagagt cttggaatgc aatgatcaaa cctggaaaga aggcgataaa
    50101 cggcccaata tatggaatga tacataacaa cccagcagaa atagctaaaa cactagcaaa
    50161 ttctaatccg atcagtaaga aacaaaccca gtagactgct ccaaggataa atgaagcaat
    50221 gatttgcccc tttagaaatg aacccaattg ttgattagca atctttgtca aatttttaaa
    50281 atctgtacga aatgctggtg ggacaatcgc taaaactgac tgataaaatt tttgaggatc
    50341 tctcaaaagg aaaaaagcga caattggacc agtaaataaa gtaataaaaa tagctgtaac
    50401 agttgaaaaa atgttgctga tccgttgtgc gccactttcc caataatttc caataaatcc
    50461 agctatatca gacgtcacgt cgtcaataga agcgaaaaat tgattaaatg aatcggcaaa
    50521 aggtgttttc tccaaaaatt gtcggagact ctcttgaaaa tcttgaaaca aatctggcaa
    50581 actactgatc aatgtctttg cctgttcttc aagaaaagga ataagccata tgcttccaag
    50641 ccccaataaa aataaaataa tcacataaac gataatgact gacaagttcc tcgaaaggtg
    50701 cttgctcaaa cgtttgatga ctggttccaa aagataatac aagatcagtg caaacaataa
    50761 tgggggcaag atagtagcga taatgattcc gacaggcttg aatataaacg agatctgatg
    50821 gaaaaaataa atgatcagcg caagtaagat aactattcct aaaacatagt aagaggtttt
    50881 tcctcctaaa aagcggatca aacgattcgt tgtatctgtt tcttttttca ctctttttcc
    50941 tcctttatct ataacttcat tatagacaaa agcgggtata aaaggaaaat gatagaggtt
    51001 gattgcaaaa atatcgtcaa tcattcatat aaactttctc atcaattaaa ataagaaaac
    51061 gcttgacagt tcttttaacc atatgataaa ttaaatcctg ttgctaaaac gtaactatta
    51121 cggtttgcga aaagcgaatc ggaacattca aatgaaagaa gatgagaaga ggagtgtcat
    51181 aaatgaaacg attgataggt atattggcca tgctggtttt agcaggtatg ttaactgctt
    51241 gcggggcaag tgggaaagcc gaggacagta aagaaaaatt gagcgtgatg acgacctttt
    51301 atcccatgta tgattttaca aaagcaatcg tcggggatga aggagaagta gagttattga
    51361 ttccagcagg gactgattcc cacgattatg aaccttctgc taaagacatg gcaaagatcc
    51421 aagatacaga tatctttgtt tataatgatg aaaacatgga aacatgggtc cccgcaatcc
    51481 aaaaaacatt acaagaaggc aatgtccata cgataaaagc gacagaaggt atgcttttac
    51541 ttccgggaag tgaagaagga catgatcatg accatgaaca cggggaagaa ggccatacac
    51601 atgagctaga cccacatgta tggctagctc caagtttagc aatcaagcag gtagcaaaca
    51661 tacgtgatca gctgattgaa gcttacccag aaaaacagga agtatggaca aaaaatgcag
    51721 cagcctatac cgagaaactt caagcactcc atcagcttta tcaagaaacc tttaagcaag
    51781 caaaacaaag atcttttgtc acgcaacatg ctgcattcaa ctatctagcg ttagaatatg
    51841 gattgaatca agtatcgatt gctggtcttt cttctagtga agaaccgtct gcggcgagaa
    51901 ttgctgaact gaaacacttt gttaaagaac atggaatcaa ctatatttat tttgaagaaa
    51961 acgccaaaga tagtattgcc agaacgttag caaacgaagc aggtgtatct ttagaagtat
    52021 tgaatccttt ggaaggattg acgaatgaac aaatagaaaa tggagaaaac tatctttcta
    52081 tcatggaagc aaacctcgaa gcgttgaaaa aaacgacaga gacagaaaac ccgctagaag
    52141 atactttgac ccccaaaaaa gaaaagagcg tctacaacgg ttattttgaa gacgaggatg
    52201 tacaagatcg ctctctttca gattgggcag gaacttggca aacagtagat acgtatgtag
    52261 aagatgggac ttttgatcct gtttttgaat ataaagaaaa attgaatcag gataagacag
    52321 cacaagaata taaagaatat tatacgaagg gctatcaaac agatattgct tcaatcaaga
    52381 tcagtgagaa agagatggca tttacgttta agaatggaac aacgaaacgt tcaaattatc
    52441 gttatgtagg gaaagaaatc ttgacctacg aggcaggaaa ccgtggcgta aggtatctat
    52501 tcgaagcaga agataaggaa agtggcgctt ttagatatat tcaattcagt gatcacacga
    52561 ttgcaccggc tgactccgaa cattttcatt tatatgcagg aaatgaaagt caagaagtat
    52621 tgctgaaaga aatggagaat tggccaacat tttatccaga tacaatggat ggacgtgaaa
    52681 ttgcacagga aatgcttttg cactgatagg caaaaaagaa ccgaagggtt tggatcggct
    52741 ggtcatttag tcggtccaaa ctattcggtt ctttttatga aatcaatttc tttaatccac
    52801 ggatagaggc tgggatggtc tcgccgtttg ccaacgtgac ttctctggtt tttttattaa
    52861 tcgaccgcac attttgtgta ttgataacat aggatttatg acaacggata aaattccgat
    52921 gcattttctc gatttctttg atcttctcat agaattgtaa ttgggcatga gtcaaatgga
    52981 cttccagttt atgtggcgct cctagcgaag tagaaaaata ttggatgtct ttgatcggta
    53041 aaaatttgat ctcatcatta aactctattt tgaagatatc tttttggatt tgggacattt
    53101 gaaaatcttc gaaaattttg ctcaggatat gctcgacatt ttcttttaaa tcataaagat
    53161 cttctttgac gatataatca gtcggttcga tgttgctttt taagattttc atcgacattt
    53221 ccgtatggga agtcacatag attatttttc ctaaaggatc taattcacgg atcaatttac
    53281 cgatcgctac accatccatt tcactgtctt ctagttcgat atctaaaaag taaataccaa
    53341 aagaagaagt tctgatcttg gctgcattga gaagttcttc cggatcagag gtagaaatat
    53401 ggacataact gtctaagttt tcaatcatga ttctattttt gatcaccgtt gataaaactt
    53461 ctaattgttt tggatcgtct tcacaaatat aaatgggaat catatcatta ctcctttatt
    53521 ttatttgtat ataaatcgtt tgagaaaaac gctggttttc aattgatgtt tcaaggaaaa
    53581 gataaggatt ggtctttgtc aattcatcca agataaacaa gccaaggcct tcgttttttg
    53641 gatttgtttt tgttgagaac cctttttgtt tcaactggtt taacggtaat gtatttgttg
    53701 ttgtattgat cacacgaatc atcacggaat gattcttctt gagtatggtc aattcgattt
    53761 ttttcttctt tgcagcacta gctccttcaa tcgcattgtc caagataata ccaagcatac
    53821 gtacaaggtt taccgtatgt ttcgtatcca gttcgattgg ctcaggaaaa tgaagctgga
    53881 cgatcaaccc tttttcctgt gccatcatga gttttaatga cagtaaacta cggatctcag
    53941 gaactttcat attatccaac atacttagat tggccggaaa aaggttcatc atctggcgtg
    54001 taggtaatat cgtttgatca taataagacc gtaatccttc taagtcgcct tctgaaatat
    54061 agctgtgaag agaaaaaagc agattttggt aatcatgttt gaattctctc aagtcttgat
    54121 agtttttttc tagatctttg atataatcct gttgcatcct gacttgttgc tctaataact
    54181 gatcagctaa tttcttatta gagattttcc aataataaac caagatgatc aggccaacac
    54241 acagatatag aatgaaaaaa ctcgtattca aagctaagac atcaggtgtt tcaccagaaa
    54301 aacgggtata aaaaataatg atatagtatg taaataatat caatgtcccg atcgtaccga
    54361 tcatacggcg gtccacagtg tgaaaccaag tctctaacga tttttttaaa caaaaagcta
    54421 ataaagcaga taaaaaagcg gataagacaa tgtgaaaata ctgcaatgaa tctcctggtt
    54481 caataaccga agatttcaaa acaaaaaaat cgccgatgct tgttaagtga tctcctaaaa
    54541 gcgacgtcaa taacccaaaa ggaagcgcgt aaaccaattg ttgacgcgaa gtcgaaaaga
    54601 agaaaaaagt aagcatcagc attcctatag caattagagc agtaaaaatc cctaaaatcg
    54661 aatagaccca tccaatcaaa atacaagaaa tcagaatgac tcccgcaaac agatatttgt
    54721 atcggatgtg atggtcaatc aataagatac aaattgtgta aatcaaatat tgcaacgcta
    54781 tggaccataa aaacataaag tacagctccc attgattttt accaaattat agcacaattg
    54841 gcgtattatt tttttaattt ttcagggatt ttgaattcag aactcatacc aaagcagcag
    54901 ccagatacaa aaaagtcaga taagttagtt aattgtttaa tgattgtttt tttcatgaaa
    54961 gattcctcca aaattcatca agtagtttgt aataggaagc aacgtagcta tttctaaaac
    55021 aatcccaagt agcaaatagg tagaaaagta agaagctgag aaaaataacg taatgagaga
    55081 aaaaattaga aacatgctga tcgccttctt taataatggg ggacgtttct cgggttcaat
    55141 gggattgatt gctgtacctc ttgatccata gcgaagcagt aacaaaaagc agctgattaa
    55201 caaaaagacc atcattggga aggagatagt caattgcaga tgtttcatca ccaagggaac
    55261 gccccataaa taaagtagtg tccacagcag acaaacgaat tcagagtcgg aatgcaatcc
    55321 ataagcatat ctgcggatgc agatataaga aagaaaggtg attcctgttt ccgcccatga
    55381 gcctgtcacc agagaaaaga ctagaacgac cgctagtttt ccaagattat tcaataaaac
    55441 ttctaagaca aattttactt ggacatacac catatcttct tccgatttgt ctccgctgaa
    55501 taaggcttct aataatttgt tcacaacttt atctttcatc tcatacgtac ctccaccaca
    55561 aattttatgc taaaaaaaat aaaaaacgtc ttttgtttca taaaaagtgg ttttgacatc
    55621 aaaaccgtca tgatctccta aaaaaatctt cgttttccaa gaaccttacc aaaaaaagtg
    55681 acagttttga tacgaaaagt actttttatg acaagtttgt gctttttcga gtgatttatg
    55741 ttaaaaaatt actgacctta taaaaggtga tggagggatt cttatggaaa atgaacaatt
    55801 gtttgaagtc ggcgaaacat atgcatttaa atctgattta tttgaatcac caataaaagg
    55861 aaaaatagaa aaaatctatg aaaatagtgt gatgatacgt gtcatgaaat gcgcagtaga
    55921 agacaatgat accgctcata atctgcactg gcagttagtt gtacgcaaat caagtacaat
    55981 agaagaataa aaacagataa aatgttgaga gcataaagag gttgtgagtc tcccgtttag
    56041 ctctgagtta caaggaataa tgttaaaaaa cagcttttca tgttttatga atattgcctt
    56101 gtgaccgagg tgttggcagg agaatactgt ttatttgaaa aaagggctgc gacaagactt
    56161 ttgtcacagc ctttattttc atgaatagga gttacttttg gctactttta gagaagtagc
    56221 ttttaaggag cagataaagt caaggctttt atttctataa atgaagtagt aaaatgaaga
    56281 acagatttta ttacaggaag gatgccgata attgtgatca ttgttgcagg aacgatcggt
    56341 gcagggaaaa gcacactaac tgaaatgctt gcccaagatt tagaaacaaa acctttttat
    56401 gagaacgtgg aagataatga agtattgccg ctattttatt ctaatccaga aaagtatact
    56461 tttttattac agattttctt tttgaacaaa cgctttttgg caatcaaaga tgctttcagc
    56521 catgatgaca acgtactaga ccgctccatc tacgaagata gtatgctgtt tcatttgaat
    56581 gcagatttag ggcgtgtttc tgaagtagaa gtaaagcagt atgaaggtct tttagaaacg
    56641 atgctgaagg agttagaaga aatttcgcca caaaagaaac cagatctact ggtttatatt
    56701 cgtgtatcct ttgaaactat gttagcgaga atcaaaaaaa gaggccggga atatgaacag
    56761 ctagaacaag atcctgaact ctattcctac tataaagaac tcaatcgacg atacgaagag
    56821 tggtatgaac agtttgatat atgtcccaaa atagtgatcg atggggacaa atatgatttc
    56881 gtagccgatc cggcatgtgg agaacaaatc gtacaagaaa tcaagatgcg ggcaaagaaa
    56941 atgacagaag aggcagatgc ccagtcgatc cgctacacta caaataaccc gaaagcaaag
    57001 ggaatcttta caactaaata atataaaaac taagcaaatt gctcactcgc agatatttct
    57061 ctgtgagcag catcttgctt agtttttttg attcattgag atcaaatagt gaaccgattc
    57121 gtatgataaa attgccataa atgaaatgtc tgagtgaggg ccatgatcaa aaataaaatg
    57181 gaaacgatca aaatcgaatg ggcgccaaac agatgatgac agaaaacaag gacaatagct
    57241 cctgcgataa aggcaagaac gacacttaga tagtggaaaa atcgatcaaa atctgctttt
    57301 tctttagaaa tacaataatc atataagttc gttgctaccc cttttaaatt tccagttgta
    57361 aataaattag tgtaggcacg cccgttgatt ttatcaaaag ctacccactg gatcgatgca
    57421 gtaaaagcaa tcaaaatcgt tactaatgcg ggatgctcat ggacgggtgt caaactaaca
    57481 aagaaaaaaa tcactgcttc ataatataga acaaacaagc gccagaaatg tttttctttt
    57541 tttctaaaat aatcaattag atactttgtt agaagaatcc ctaagaaaaa gaaaagggta
    57601 gacaatactt ttttaccaac acttagccat tctccgtcaa atgcttggat gatcgataga
    57661 atgatattgc cagtttgagc agaggcaaaa gcaccatatt ggatataagt ataactgtcc
    57721 atggctcctc cgataaatgt cagcaaaagt cctattgtac gcccctcgtg aaaggaagtc
    57781 tgcatttttt tcatcacctt ttatgaatat ttttgtcttc cttatcttag cataaacgat
    57841 tgaaaaaaaa aggaaaaatt ttcagaaaac taaaatcata tcgcttccca ttttcatttt
    57901 tagaaaggtc tgttagtata aagaaaaaat agacaacgaa actttaaata accaacttga
    57961 aataaacaaa ttcaaagaag aaagatggga aggaaatcaa tcagttgaat gaaaaagaaa
    58021 ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa gtataaaaag cttcaacaag
    58081 tctttcggct acaagaagaa atcattgcta cctatgaagc ggcttatggc gaagcacccg
    58141 gacttattga ggaagaagta caagtaaaag aaagtgaagg aaacagctct ttctttaaaa
    58201 tgatggagaa acgttcctat aatcgcttca atcaagaaca aaaagcagct atcacatatg
    58261 atatgaagaa acatcttcga atcatagcag gagctgggag cggcaaaacg caaacaatct
    58321 gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga cgaagaacgc atactaatga
    58381 tcacttttac aaggaacgct gcaaatgaac taaaaaaacg agtagataat ttcagccaga
    58441 gaaaaaccgc tgtccatatc ggaacattcc atagtatctt ttttaggata tacaatgaaa
    58501 tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca aggagacttt tcaaaggata
    58561 ctgcacaaaa agtgaatgct gttttgcaac agctgatacg taaatataat ctttatatat
    58621 ttgatcaata tggagaaaaa acgattgctt ctcggttaga ctattggcag aatatgaacc
    58681 tttcagtaga agaaatggtt cagttagtta aagaaaaata tgattccatt gataagaatg
    58741 cacgacagcc gatatcagaa cgactgtatg gattgctcac ggaactccaa gaacaaaaaa
    58801 gaagccagca attgctagag ttcaatgatg tactccaaaa tctgaaaagc gctttggaga
    58861 atgaggagat ccagcattat atcggccaaa aatatgatta tctattcatt gatgaatttc
    58921 aagatactaa tccacttcaa tgggaaatcg tgaaacggat gacaaaggaa aacaggatca
    58981 agttgattgt tgttggagat gatgatcaga gtatctatgg attcagagga tcagagccag
    59041 catacatcaa gaactttgaa aaagaatatc ctaccaaaac tcttttctta ttaactaatt
    59101 atcgttctcg ggcagctatt gtccaaggag caaatagatt gatcagctat aataaaaatg
    59161 atcggatacc aaaggcgatg attccagcac aaaaagaagc aggtgttatg gaagcctatt
    59221 tgttttctga tactaaagca gaaagtcagt ggctaatcaa acaaattcag acatttattc
    59281 aaaaaaatgg gaaatataag gaatcaatca tattataccg atcagcttca caaacgcagc
    59341 agcttgtcca atctctgctg aaaacgaata ttccttttgt actggaagca gatagtccat
    59401 atgaaggtat ctttggtatc aagtctttcc aacatttcta tcaaaaaatt gttcattggc
    59461 aaacagcact gaatctgcaa aagaaaaatc aggcctacca gcaattgctc cggcagctga
    59521 tggcagactg ctacctgaaa aaaagtgaat gcgatcaata cttttctcct aaatatcaaa
    59581 atatagccgt tgcagattat attttgtcga taagccccaa tttaaaatcg aaatctgcag
    59641 aaatccgaga gtttgaggaa gcgttgcgta aaacagacca gcatcaagaa atctattcgc
    59701 ttgttcaaag ctatcttcat ctgccacgta tagctaagga aatcgaccag agtgatcaag
    59761 aatggatacg agaagaagta aagcagcatg ctactttcag aagactccaa gccttatcgc
    59821 aagaaaccca agcgacagct aaagaattaa aaacccgctt gattgcttac agacaaggaa
    59881 aacttgatgc actatgtatc cagagcattc acaaaagtaa aggtcttagt taccgaaatg
    59941 tctttttgat tggttgtaac gaaggaagtt tgccttataa cggagcgaca gaaaagaaag
    60001 taatcgactt taatgaaata aaagcagaac cagtaacgac gatagaagaa gaacgaagac
    60061 tattttatgt tgcgatgact agagcaagaa atcgtctgta tctatgtgta cctcaaatga
    60121 aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga aaccgggagt cgtgtgaaaa
    60181 aaggaaagtg atagaagaga gggtgtgaca gaagtggtca gtttcaagaa agaagaagga
    60241 atgtacgaaa attgcttctc aaatttttgt gaaatttcag cttattttcg aagaagctat
    60301 ttctggaaca
//
LOCUS       DAZKOR010000004        60302 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000004 DAZKOR010000000
VERSION     DAZKOR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.004"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..807)
                     /locus_tag="ACG8ZM_000172"
     CDS             complement(<1..807)
                     /locus_tag="ACG8ZM_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0491170.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKK"
     gene            complement(1031..2971)
                     /gene="thrS"
                     /locus_tag="ACG8ZM_000173"
     CDS             complement(1031..2971)
                     /gene="thrS"
                     /locus_tag="ACG8ZM_000173"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0491171.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3308..3487)
                     /locus_tag="ACG8ZM_000174"
     CDS             complement(3308..3487)
                     /locus_tag="ACG8ZM_000174"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0491172.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3534..5357)
                     /locus_tag="ACG8ZM_000175"
     CDS             complement(3534..5357)
                     /locus_tag="ACG8ZM_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0491173.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5635..7914
                     /gene="helD"
                     /locus_tag="ACG8ZM_000176"
     CDS             5635..7914
                     /gene="helD"
                     /locus_tag="ACG8ZM_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0491174.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8007..8654)
                     /locus_tag="ACG8ZM_000177"
     CDS             complement(8007..8654)
                     /locus_tag="ACG8ZM_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491175.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8833..9552)
                     /locus_tag="ACG8ZM_000178"
     CDS             complement(8833..9552)
                     /locus_tag="ACG8ZM_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0491176.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9683..10834)
                     /locus_tag="ACG8ZM_000179"
     CDS             complement(9683..10834)
                     /locus_tag="ACG8ZM_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0491177.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11051..11791)
                     /locus_tag="ACG8ZM_000180"
     CDS             complement(11051..11791)
                     /locus_tag="ACG8ZM_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0491178.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11795..12118)
                     /gene="rsfS"
                     /locus_tag="ACG8ZM_000181"
     CDS             complement(11795..12118)
                     /gene="rsfS"
                     /locus_tag="ACG8ZM_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0491179.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12175..12768)
                     /gene="yqeK"
                     /locus_tag="ACG8ZM_000182"
     CDS             complement(12175..12768)
                     /gene="yqeK"
                     /locus_tag="ACG8ZM_000182"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0491180.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12752..13402)
                     /locus_tag="ACG8ZM_000183"
     CDS             complement(12752..13402)
                     /locus_tag="ACG8ZM_000183"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0491181.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13412..13723)
                     /gene="yhbY"
                     /locus_tag="ACG8ZM_000184"
     CDS             complement(13412..13723)
                     /gene="yhbY"
                     /locus_tag="ACG8ZM_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0491182.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13739..14848)
                     /gene="yqeH"
                     /locus_tag="ACG8ZM_000185"
     CDS             complement(13739..14848)
                     /gene="yqeH"
                     /locus_tag="ACG8ZM_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0491183.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14845..15372)
                     /locus_tag="ACG8ZM_000186"
     CDS             complement(14845..15372)
                     /locus_tag="ACG8ZM_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0491184.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15552..16337)
                     /locus_tag="ACG8ZM_000187"
     CDS             complement(15552..16337)
                     /locus_tag="ACG8ZM_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0491185.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16330..17199)
                     /gene="accD"
                     /locus_tag="ACG8ZM_000188"
     CDS             complement(16330..17199)
                     /gene="accD"
                     /locus_tag="ACG8ZM_000188"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0491186.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17200..18579)
                     /locus_tag="ACG8ZM_000189"
     CDS             complement(17200..18579)
                     /locus_tag="ACG8ZM_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0491187.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18587..19006)
                     /gene="fabZ"
                     /locus_tag="ACG8ZM_000190"
     CDS             complement(18587..19006)
                     /gene="fabZ"
                     /locus_tag="ACG8ZM_000190"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0491188.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19031..19507)
                     /gene="accB"
                     /locus_tag="ACG8ZM_000191"
     CDS             complement(19031..19507)
                     /gene="accB"
                     /locus_tag="ACG8ZM_000191"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0491189.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19510..20745)
                     /gene="fabF"
                     /locus_tag="ACG8ZM_000192"
     CDS             complement(19510..20745)
                     /gene="fabF"
                     /locus_tag="ACG8ZM_000192"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0491190.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20764..21501)
                     /gene="fabG"
                     /locus_tag="ACG8ZM_000193"
     CDS             complement(20764..21501)
                     /gene="fabG"
                     /locus_tag="ACG8ZM_000193"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0491191.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21513..22430)
                     /gene="fabD"
                     /locus_tag="ACG8ZM_000194"
     CDS             complement(21513..22430)
                     /gene="fabD"
                     /locus_tag="ACG8ZM_000194"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0491192.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22537..22761)
                     /locus_tag="ACG8ZM_000195"
     CDS             complement(22537..22761)
                     /locus_tag="ACG8ZM_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0491193.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22814..23776)
                     /locus_tag="ACG8ZM_000196"
     CDS             complement(22814..23776)
                     /locus_tag="ACG8ZM_000196"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0491194.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23786..24226)
                     /locus_tag="ACG8ZM_000197"
     CDS             complement(23786..24226)
                     /locus_tag="ACG8ZM_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0491195.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24532..25497
                     /locus_tag="ACG8ZM_000198"
     CDS             24532..25497
                     /locus_tag="ACG8ZM_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0491196.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25554..25784)
                     /locus_tag="ACG8ZM_000199"
     CDS             complement(25554..25784)
                     /locus_tag="ACG8ZM_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0491197.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25870..26757)
                     /locus_tag="ACG8ZM_000200"
     CDS             complement(25870..26757)
                     /locus_tag="ACG8ZM_000200"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0491198.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26846..28069)
                     /locus_tag="ACG8ZM_000201"
     CDS             complement(26846..28069)
                     /locus_tag="ACG8ZM_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0491199.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28086..29132)
                     /locus_tag="ACG8ZM_000202"
     CDS             complement(28086..29132)
                     /locus_tag="ACG8ZM_000202"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0491200.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29296..31023)
                     /gene="ptsP"
                     /locus_tag="ACG8ZM_000203"
     CDS             complement(29296..31023)
                     /gene="ptsP"
                     /locus_tag="ACG8ZM_000203"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0491201.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31023..31289)
                     /locus_tag="ACG8ZM_000204"
     CDS             complement(31023..31289)
                     /locus_tag="ACG8ZM_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0491202.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31768..34002
                     /locus_tag="ACG8ZM_000205"
     CDS             31768..34002
                     /locus_tag="ACG8ZM_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0491203.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34150..34470
                     /locus_tag="ACG8ZM_000206"
     CDS             34150..34470
                     /locus_tag="ACG8ZM_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0491204.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34675..34899)
                     /locus_tag="ACG8ZM_000207"
     CDS             complement(34675..34899)
                     /locus_tag="ACG8ZM_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0491205.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35097..36677)
                     /locus_tag="ACG8ZM_000208"
     CDS             complement(35097..36677)
                     /locus_tag="ACG8ZM_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0491206.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37078..38454)
                     /locus_tag="ACG8ZM_000209"
     CDS             complement(37078..38454)
                     /locus_tag="ACG8ZM_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0491207.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38582..39670)
                     /locus_tag="ACG8ZM_000210"
     CDS             complement(38582..39670)
                     /locus_tag="ACG8ZM_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0491208.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            39842..40276
                     /locus_tag="ACG8ZM_000211"
     CDS             39842..40276
                     /locus_tag="ACG8ZM_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0491209.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40354..40887)
                     /locus_tag="ACG8ZM_000212"
     CDS             complement(40354..40887)
                     /locus_tag="ACG8ZM_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0491210.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(40981..42159)
                     /gene="mutY"
                     /locus_tag="ACG8ZM_000213"
     CDS             complement(40981..42159)
                     /gene="mutY"
                     /locus_tag="ACG8ZM_000213"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0491211.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42152..42949)
                     /gene="recX"
                     /locus_tag="ACG8ZM_000214"
     CDS             complement(42152..42949)
                     /gene="recX"
                     /locus_tag="ACG8ZM_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0491212.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43056..44438
                     /gene="rlmD"
                     /locus_tag="ACG8ZM_000215"
     CDS             43056..44438
                     /gene="rlmD"
                     /locus_tag="ACG8ZM_000215"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0491213.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44612..45190)
                     /locus_tag="ACG8ZM_000216"
     CDS             complement(44612..45190)
                     /locus_tag="ACG8ZM_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0491214.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45512..45982
                     /locus_tag="ACG8ZM_000217"
     CDS             45512..45982
                     /locus_tag="ACG8ZM_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0491215.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46090..47316)
                     /locus_tag="ACG8ZM_000218"
     CDS             complement(46090..47316)
                     /locus_tag="ACG8ZM_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0491216.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48070..48270)
                     /locus_tag="ACG8ZM_000219"
     CDS             complement(48070..48270)
                     /locus_tag="ACG8ZM_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0491217.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48404..49012)
                     /locus_tag="ACG8ZM_000220"
     CDS             complement(48404..49012)
                     /locus_tag="ACG8ZM_000220"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0491218.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49097..49567)
                     /locus_tag="ACG8ZM_000221"
     CDS             complement(49097..49567)
                     /locus_tag="ACG8ZM_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0491219.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49615..50529)
                     /locus_tag="ACG8ZM_000222"
     CDS             complement(49615..50529)
                     /locus_tag="ACG8ZM_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0491220.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50675..51478
                     /locus_tag="ACG8ZM_000223"
     CDS             50675..51478
                     /locus_tag="ACG8ZM_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0491221.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51656..52603
                     /locus_tag="ACG8ZM_000224"
     CDS             51656..52603
                     /locus_tag="ACG8ZM_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0491222.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(52671..52964)
                     /locus_tag="ACG8ZM_000225"
     CDS             complement(52671..52964)
                     /locus_tag="ACG8ZM_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0491223.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(52992..53657)
                     /locus_tag="ACG8ZM_000226"
     CDS             complement(52992..53657)
                     /locus_tag="ACG8ZM_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0491224.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(53797..54429)
                     /locus_tag="ACG8ZM_000227"
     CDS             complement(53797..54429)
                     /locus_tag="ACG8ZM_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0491225.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(54436..55503)
                     /gene="liaS"
                     /locus_tag="ACG8ZM_000228"
     CDS             complement(54436..55503)
                     /gene="liaS"
                     /locus_tag="ACG8ZM_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0491226.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(55500..56231)
                     /gene="liaF"
                     /locus_tag="ACG8ZM_000229"
     CDS             complement(55500..56231)
                     /gene="liaF"
                     /locus_tag="ACG8ZM_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0491227.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(56400..56879)
                     /gene="greA"
                     /locus_tag="ACG8ZM_000230"
     CDS             complement(56400..56879)
                     /gene="greA"
                     /locus_tag="ACG8ZM_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0491228.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(57015..58190)
                     /gene="mltG"
                     /locus_tag="ACG8ZM_000231"
     CDS             complement(57015..58190)
                     /gene="mltG"
                     /locus_tag="ACG8ZM_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0491229.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            58394..59563
                     /locus_tag="ACG8ZM_000232"
     CDS             58394..59563
                     /locus_tag="ACG8ZM_000232"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0491230.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(59830..>60302)
                     /locus_tag="ACG8ZM_000233"
     CDS             complement(59830..>60302)
                     /locus_tag="ACG8ZM_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491231.1"
                     /translation="VVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLK
                     NHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQ
                     GGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT        17509 a        12645 c        10160 g        19988 t
ORIGIN      
        1 ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc
       61 gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt
      121 tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg
      181 cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc
      241 tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag
      301 ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca
      361 tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt
      421 ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac
      481 tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga
      541 ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat
      601 cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt
      661 cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc
      721 aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat
      781 actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt
      841 aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa
      901 tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct
      961 ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga
     1021 aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa
     1081 agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg
     1141 catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg
     1201 cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca
     1261 tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga
     1321 tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta
     1381 aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat
     1441 gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt
     1501 ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta
     1561 gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat
     1621 ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt
     1681 tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt
     1741 cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag
     1801 gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca
     1861 aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt
     1921 cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc
     1981 gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat
     2041 gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat
     2101 gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg
     2161 ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca
     2221 agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga
     2281 tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt
     2341 gattgtttga gtttccacgc caataagcac ctgccactga taataattta aatacttgta
     2401 tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat
     2461 aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac
     2521 ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt
     2581 ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt
     2641 tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt
     2701 ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga
     2761 ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga
     2821 ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta
     2881 agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga
     2941 ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt
     3001 gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag
     3061 gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta
     3121 acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc
     3181 ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct
     3241 ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa
     3301 actaaactta ttttttccat tccccattta caccgtatgt tccttttttc atctcattga
     3361 tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat
     3421 ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga
     3481 atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact
     3541 atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca
     3601 ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt
     3661 agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac
     3721 tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga
     3781 tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc
     3841 attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc
     3901 atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc
     3961 aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca
     4021 gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt
     4081 gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga
     4141 tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag
     4201 gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg accggtcact
     4261 tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga
     4321 gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt
     4381 tttattccat tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct
     4441 gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt
     4501 acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct
     4561 agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt
     4621 gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa
     4681 cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata
     4741 atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct
     4801 ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca
     4861 cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt
     4921 acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata
     4981 tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac
     5041 tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc
     5101 atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg
     5161 gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa
     5221 aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc
     5281 tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat
     5341 tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata
     5401 aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat
     5461 aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa
     5521 caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga
     5581 aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat
     5641 gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa
     5701 aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac
     5761 acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa
     5821 tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa
     5881 cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa
     5941 gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa
     6001 gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt
     6061 ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga
     6121 caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat
     6181 gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc
     6241 tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg
     6301 attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg
     6361 ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat
     6421 ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca
     6481 cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag
     6541 caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga
     6601 ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg
     6661 ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg
     6721 tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag
     6781 ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat
     6841 ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa
     6901 caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt
     6961 cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac
     7021 tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg
     7081 caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat
     7141 gctgtactat tctttttact gatgcgccga acatatccag tgacggttac acacaaagct
     7201 cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa
     7261 gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga
     7321 aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc
     7381 caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt
     7441 gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa
     7501 ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga
     7561 aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt
     7621 tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac
     7681 tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac
     7741 cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc
     7801 ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa
     7861 agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa
     7921 agacaactgc tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa
     7981 aaagtaattc aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga
     8041 tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct
     8101 gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga
     8161 ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc
     8221 aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg
     8281 tctatcttct catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct
     8341 aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca
     8401 gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg
     8461 tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga
     8521 tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc
     8581 acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg
     8641 atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga
     8701 tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa
     8761 gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt
     8821 aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag
     8881 atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac
     8941 ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt
     9001 taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc
     9061 ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc
     9121 agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc
     9181 ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg
     9241 attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc
     9301 atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc
     9361 aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg
     9421 gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt
     9481 tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca
     9541 tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc
     9601 ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt
     9661 cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg
     9721 gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg
     9781 cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc
     9841 ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct
     9901 cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat
     9961 atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg
    10021 ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag
    10081 tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac
    10141 aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt
    10201 ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat
    10261 gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat
    10321 tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat
    10381 cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag
    10441 acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac
    10501 tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg
    10561 tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga
    10621 cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt
    10681 tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga
    10741 cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag
    10801 gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat
    10861 aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat
    10921 aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc
    10981 tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca
    11041 ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg
    11101 cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc
    11161 ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc
    11221 gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct
    11281 cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat
    11341 attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat
    11401 tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca
    11461 tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat
    11521 cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg
    11581 caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa
    11641 aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt
    11701 gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat
    11761 cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga
    11821 ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga
    11881 aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt
    11941 caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg
    12001 cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa
    12061 catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg
    12121 caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt
    12181 ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc
    12241 aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca
    12301 tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc
    12361 tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat
    12421 tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac
    12481 aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac
    12541 gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc
    12601 aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc
    12661 tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag
    12721 gttttctcta gaataagcag tatattcatt actatagtcc atattcatac aacccttcat
    12781 tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga
    12841 tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag
    12901 gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag
    12961 tcagctcatc gattttatac cattttggca aatattctac catatcccca ccgataataa
    13021 agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac
    13081 ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg
    13141 ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag
    13201 gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt
    13261 cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt
    13321 gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg
    13381 tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc
    13441 tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga
    13501 cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg
    13561 tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa
    13621 cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg
    13681 ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt
    13741 aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta
    13801 ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca
    13861 cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct
    13921 gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat
    13981 gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat
    14041 caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag
    14101 gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca
    14161 tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa
    14221 tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc
    14281 ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga
    14341 cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct
    14401 catgagattt cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag
    14461 cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca
    14521 ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta
    14581 gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta
    14641 attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat
    14701 aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca
    14761 aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac
    14821 agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg
    14881 ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca
    14941 actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc
    15001 acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa
    15061 ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa
    15121 aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac
    15181 aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc
    15241 tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag
    15301 attctttaat tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta
    15361 atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc
    15421 aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca
    15481 gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa
    15541 acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga
    15601 tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact
    15661 ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct
    15721 aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta
    15781 ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct
    15841 tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca
    15901 ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca
    15961 atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg
    16021 atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt
    16081 cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt
    16141 cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca
    16201 atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc
    16261 tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca
    16321 tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg
    16381 gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa
    16441 gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg
    16501 tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc
    16561 aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc
    16621 ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat
    16681 tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt
    16741 tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat
    16801 gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc
    16861 agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata
    16921 ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt
    16981 ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg
    17041 agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca
    17101 ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg
    17161 attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt
    17221 ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc
    17281 tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc
    17341 tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc
    17401 atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta
    17461 aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt
    17521 ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc
    17581 gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc
    17641 cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg
    17701 gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat
    17761 cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc
    17821 ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt
    17881 gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa
    17941 gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt
    18001 tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt
    18061 tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg
    18121 ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact
    18181 tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc
    18241 cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat
    18301 ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt
    18361 tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc
    18421 cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc
    18481 tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc
    18541 gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa
    18601 aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc
    18661 cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa
    18721 ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt
    18781 gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc
    18841 ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat
    18901 ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca
    18961 ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt
    19021 cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact
    19081 cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca
    19141 gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt
    19201 tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa
    19261 cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg
    19321 cttgttcagg tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca
    19381 tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca
    19441 aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg atttcattga
    19501 tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc
    19561 taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt
    19621 caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc
    19681 taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt
    19741 catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat
    19801 tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc
    19861 gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc
    19921 agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac
    19981 gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag
    20041 aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc
    20101 acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat
    20161 ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat
    20221 gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt
    20281 acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc
    20341 cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg
    20401 gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg
    20461 attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc
    20521 atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg
    20581 gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat
    20641 cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc
    20701 aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt
    20761 ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg
    20821 attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat
    20881 cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt
    20941 tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc
    21001 cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat
    21061 atttccaatc aacccagata cactagagag attaatgatg gccccttctc gctgcttcat
    21121 catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca
    21181 ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt
    21241 gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc
    21301 tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat
    21361 ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc
    21421 ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac
    21481 attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat
    21541 gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg
    21601 cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta
    21661 tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt
    21721 gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag
    21781 aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta
    21841 tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt
    21901 aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta
    21961 taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt
    22021 tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc
    22081 atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta
    22141 gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca
    22201 tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat
    22261 tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga
    22321 atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc
    22381 ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc
    22441 aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt
    22501 caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta
    22561 agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc
    22621 atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg
    22681 gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc
    22741 ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt
    22801 ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc
    22861 ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa actgcttcat
    22921 ccaataagat agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga
    22981 acttttcttg cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct
    23041 gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa
    23101 cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt
    23161 ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac
    23221 ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta
    23281 agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga
    23341 ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg cagcggatca
    23401 ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag
    23461 cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt
    23521 aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct
    23581 gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac
    23641 atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct
    23701 tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca
    23761 ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt
    23821 tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga
    23881 ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc
    23941 ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta
    24001 ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt
    24061 ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg
    24121 acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga
    24181 agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac
    24241 taattagttt gattatcaaa tcttttgatg ttcaaactat atcttaactt ttttcaaaat
    24301 gcaaccttaa aagatcattt ttttactata tgagcttttt ttaagatttt actcatttaa
    24361 aacaactgat aaatcagcat ttataatttc ttttattaga aaaatcatct atctcacata
    24421 ttttgattat caaagtattt tctgtgaaca gaactataga attccctaaa acttgtccat
    24481 tatttcaaaa tttgttatag tctttttatt caaagcgtaa aaggagatcc tatgttaaaa
    24541 gaaaaaatga aaactctccc catgctgccg ggtgtctatc ttatgaaaga ccgagatggt
    24601 aaggtcatct atgttggtaa agcaaaacgg ctcaaaaata gagtaagcag ctattttcat
    24661 cagaataaac agcattcaaa aaaagtatta cgtatgattc atcatataac agattttgat
    24721 ttcgtcgtag tagacacgga attagatgct ttattgctcg agtgtcaatt gatccaacat
    24781 taccgtcctt tttataaccg acaaatgaat tatttcagta attataatta tgtacatatt
    24841 accaataagg gatttgtatt aacagatact cctacggctc gaacatacgg gccttttcgt
    24901 ctctataaaa aaatgccgtc tattttgcgt ataatggaag aaacttatca aatgccttgg
    24961 ttgtctgaaa ttagtttgtt agctttgcga gtccagcttc ctgacctaca ggaaatgact
    25021 tttgaacaga aaaaaaagga actccaaggg ctgtttcaag gccgaaataa aaagctactg
    25081 acttacttga aaaaaagaca acaacatttc atctatcagt tgaatttcga aaaagccggt
    25141 atgcttcaaa aagatatcga attagttact tattttatta gacggatcca ggaacaaaag
    25201 cagtttttaa gaacaccatc gctcaccttc agcatgccat tagcagcaga tgagagtcag
    25261 aaaaagcact acctcgtttg ttatggtcaa cttgcagaaa caatgattac ctctggtgac
    25321 gtttctcctg acttctatta tgaaaagaaa gaagctcatt tatctttgaa acgtcaattg
    25381 tcaaaagaag aaatcgatcc tgtacaaatc ttgatcagtt accgcaaaaa attagaaaaa
    25441 gaacagtttg aaattgaact gttaaacaaa aaagaagctg aaaagcaact aaattagttg
    25501 cttctcggct tcttttaggc atcttcaact tcaaatgtca attatcctaa aatttattgg
    25561 attaagtcat agatttccat cgctacgata tcgatattat caaattcgta ttgctgatga
    25621 gtatctgttt cagttaattc aaatgtatcg gtcgctttat cataagttac tgtacacttc
    25681 tctacgcctt ctttttcaaa acggcgaact tgcagttcat tatctgctga ctcgaccata
    25741 gcctccagtc gttttatgat tgcgactaat tgtgaatgtg tcatcggagg tcctcctttt
    25801 cttatttctg ttatattgta tcaaaagcat agcctacttg cattgatttt ctctaaaaaa
    25861 actcgttttt tatgattttt tttctggtat ttttccttca ggccaccaaa gtttgatcaa
    25921 agcttgggtt ccgtcattct caaaaccttt atctgccaac tcttcatata gtttcaatgc
    25981 ttgggtagtt gctggcaata caagatccat tttttctgct tcttctaatg caatctttaa
    26041 atctttgata aaatgtttga caaaaaaacc aggtgtgtaa tcttctttta aaatacgagg
    26101 agaataattg cttaaagacc agttagctgc acttccacct gaaagcgtct caatcacttt
    26161 ctttaaatca agtccgtttt tttgtgcata gacaagcatt tccgtcattc ctgtcatcgt
    26221 acctgcaatc atgatctgat tagccatttt tgtatgctga cctttcccag cagaaccatg
    26281 caatgtaaat gtcgtaccaa attctttgaa taaaggcagt acttgatcat aaacggcttc
    26341 ttctccaccg accatgatcg tcagtgtacc attctttgct cctaagtcac caccggatac
    26401 cggtgcatct aacgcatctg cgcctttttc ttttgctgtt tttgcaattt tttctgccag
    26461 gcttggtgta cttgtagtca aatcaactaa aatatgtcct gagatatctg cagaaaaaat
    26521 ccccgactgt ccatagtaga tttcttctac atctttgggg taacctacca ttgtaaagag
    26581 aatattactt tgttctgcta ccgcttttgg tgtatctgcc cagatagctc cttctgttac
    26641 taaaggatca gcttttgctt ttgtccgatt atagacagct acttcgtgtc ctgcttctag
    26701 tagatggcgt gcgacagcac ttcccatgac gcctgttccg ataaaaccaa gtttcatttt
    26761 ttcgacctcc taaaaatcaa aaatccttgc ttctatgcta gtatacgcta gaaacaagga
    26821 ttttttcata aaaaatgttt gaattttatc ttttaaatga atttaatttt attttgattc
    26881 gttcaatcga gtcttcttct tcttttcttc gcatctcttc ttcatgataa acaaccatgt
    26941 cttgataaaa ttcgatcata gcttcgccga aatgtgtaga tgaaatatca taaagtttag
    27001 cttctaaaac ttttggatct ttaggaactt cactggaaat ataatcaata agtgctggtg
    27061 caaagtcttc gtcggtttca aaagttttcc ctaatgaagg atgatcgaac aatcggttca
    27121 aataagcatt tccttccacg acacaaggtg ttccggctgc catcgcttct gtataagtaa
    27181 gcccttgtgt ttctgaagta gaagcactga caaaataatc tgccgcttta taataaagtg
    27241 ctacatcatt gttgtccact tctcctgtga actgcacaaa ttcttctaac tgcagttctg
    27301 agatcaattc ctctaactct tctacataag gtccttttcc tacgataact aatctggcgt
    27361 taggataagc ggagatgacc tgaggaaacc cattaatcaa ggcttggata tttttctcat
    27421 aagaaatcct gcttaaagaa agaatcatca aatggttttc ctgaagacct agtgactccc
    27481 ttaatttgct gatatcctgc tgagtgatat cagaacgttt gaatttttca atgtcgatac
    27541 cagtaggaat cacgcgaagc ggtgctatta caccatagct gcgtaatgta tcaataacac
    27601 gttcacttgg acaaactaca cctgtcgtat gatttgcaaa taatctagac aagtatttta
    27661 catgggattg cctgatcact tttcctttac cgatataatg aaggtagtct tcatacatcg
    27721 tatggtaagt atggatcaga ggtattttca acttcttagc taccatcttc ccaagtatcc
    27781 ctgctccgaa ttctgtatgg gtatgaataa gatctagttc tagttctttg gcaaccaaat
    27841 aagcatacca catgcctcta acaacaactc ttcgatcctt gaaagaaata aatggtacac
    27901 ttggcattcg aatcacatct ttttccaact tatccgcatt aggatcggtc gttgtaaaaa
    27961 tatagacttc atgcccatgt ctttctaatt cttgttttaa tgttttgatc gatgtagcaa
    28021 ctccgctgac ttgcggaaag taggtatcgg taaaaaagcc gattttcatg tttttcgcct
    28081 ccaaactagt ccttgacctg atttactttt tttaatttgc tagagacttt ttcattcagt
    28141 acactttcgt atacattttt aagttcttca ccgattttat tgatacttcg ctcttttgct
    28201 gtttgataac cggcttgtgt cgtactcggt agttcttgat tggtaatctg ttggatcagt
    28261 cggataaatt cttcgtttgt cttccccata tagcagttct tgctatcttc taaccagcca
    28321 ttatacactg gaatatctcg aacaagtacg ttttgtttgc ttgctaaagc ttctaaaaca
    28381 acgatccctt ctgtctcttc ataagaaggg aaaaagaata gatctgcacc tgaatacgca
    28441 ccttctatga tctctccacg aatgtaacca ggaaacagga cattttcagg atgatcttct
    28501 tttacaattt tccggatgtt ccgaggaatc gagtacatgg gaacatgacc aaaccaaata
    28561 aatgtgtatt cgggcatctt cttagcaatt tctacgaaat caatgattcc tttacgttcg
    28621 aaaaacaaac cgacacaaat gattactttt tgatccggag acagtttgaa atattctctg
    28681 aacttttgtt ctttcattgg atcagggtga tatttctcta agtcgatccc attggaaata
    28741 gcttgaatcg gcacacggat accatagctt tccaacaact gttttgaata aggtgttggc
    28801 gtgatcagat agtccgcttt ttggtacaat cctaccagat atttttttac gataggagaa
    28861 agctggttcg atctgataaa ggaatttcgg aaatcttcct ctgtagaatg cgcatgatag
    28921 acaacttttt ttccttctct tctggcttta ttgaccatat tatgactatt gattccgtat
    28981 gtattgatgt gcaggatgtc ataatcttct ttttcatcta gtgtataaga gataccgact
    29041 tccgtcagag cacgtttttg atgatcaagc gcacgcccga ttccagattt tgcaagcatc
    29101 ttttcacttt caaaatataa taataccttc accttacgtc ccccttcact tatatagtat
    29161 aacatagcct acaaactgtc actcaaactt aagccctatt tttgtctatt cttactgaca
    29221 aaaacgtttt cttaaaaaag aacaaaaaaa ctggcgtagc tcttttacaa gccacaccag
    29281 cttttgatac gttgtttatt ttacatattc atgaacaagt tcaacgactt cttccatcat
    29341 atcgcattct ttcaatgcac gatcagcaag ttcagccatt ttcgctgtgt ccaagcgttt
    29401 cattaaacta cgcgttttaa gaatagatgt cgcactcatt gagaactcat ctagacccat
    29461 acctactagc aatggaacag ctgtttgatc tccagccatt tctccacaca tacctgccca
    29521 ttttccttca gcgtgtgctg catcaattac atttttgatc aaacgtaaaa ttgatgggtt
    29581 atatggttgg tacaagtaag aaacgcgttc gttcatacgg tctgctgcca ttgtgtattg
    29641 aatcaaatcg ttcgtaccta cagagaagaa atctacttct tttgcaaact tgtctgctaa
    29701 gactgctgct gctggaatct cgatcatgat gccgacttgg atcgtatcag atacttcctt
    29761 gccttcactt atcaattttt gtttttcttc ttcaaagatc gcttttgctg cgcggaattc
    29821 tttgagtgta gctaccatag ggaacatgat acgtaaatta ccatgaacag atgcacgaag
    29881 caatgcacgc atttgtgtac ggaacatttc gtcgccttgt tcagataaac tgatacgcaa
    29941 tgcacgatat cctaagaatg gattcatttc atgaggtaat tgcagatatg gtagttcttt
    30001 atctccaccg atgtccattg tacgaacaac tacaggtttt ccttccatac cttctaatac
    30061 agctttgtaa gcttcatatt ggtcatcttc tgttgggaag tctggagaat ccatatacag
    30121 gaattcagta cggtacagac caactgcttc tccaccatta ttatggacac caactagatc
    30181 ttttggtgtt ccgatgtttg cagctaattc aaaatgcttg ccgtcagctg tgacagtttc
    30241 tgcatttttt aatttttccc attctgcttt ttgtgctgcg taatctgcac ctgctttttc
    30301 aaattcagct ttttgttcat ctgtaggatc gatgattaca tcgccttcga tcccgttgac
    30361 agccaaaata tcgccttctt tgactttagc agtaatttct tttgtcccaa cgattgctgg
    30421 aatttctaaa gaacgtgcca tgatagcaga atgtgaagta cgtccaccaa tgtctgttac
    30481 aaacgctttt acaaaattgc gatctaattg tgctgtatca cttggagtca agtcatgtgc
    30541 aacaacgacc acttcttcgt tgatcattga tggatttggt aaagtaacgc ctaataagtg
    30601 cgctaaaata cgtttagcaa cgtcacgaat atctgcagca cgctcttgca tataagcatt
    30661 atcttccatt gcttcgaaca taccgatata catatccgtt acttctttta gcgctgattc
    30721 agcatttacg ctgttgtctt taatattttg cttgatttga cctaccattt ctggatctga
    30781 aagaaccatc aaatgggcat caaatacttg cgcttctgct tcacctaagc tttgcgctgc
    30841 tttttcacga atttgctgca attcttcagt agatttagct aatgcagcat ccaaacgagc
    30901 ttcttcagcc gcagtatctt ctactgttgc cttactgaat gttaaatccg gttgaactag
    30961 caggtaagct tttgcaacgg ctacaccgtc actagcggca atccctttta gcatttcaac
    31021 cattattcag ataagccttc tttcttcatt gtttcaacga ttgctgccat tgcatcagct
    31081 tcgtcagcac cttctgcagt aatagtaaca tcagaacctt gacctacacc taaagacata
    31141 acgcccatga ttgatttcaa gtttactgat ttacctttat actctaagtt tacatcagag
    31201 ttaaatttgc ttgctgtttg tactaatagt gtagctggac gagcgtggat cccagtttct
    31261 gctactacgt gaaattcttt cttttccata ttaatcggtc tcctttaagt gagtgttgtt
    31321 tttatgtgag ggttttcttt tttagtaatt cagagaatcc taacaaatgc caccctttca
    31381 tctgtaaaga ttaacatttt ttttcgttag atacaacagt tttcattcaa tttcagataa
    31441 aagtattcaa attattccgg attctttttc atccttttct tcacatttta taacaaacgt
    31501 gtttatttcc tttttcttgt cgctttctct tctcctgacg tttatcattc tttcctttga
    31561 ccctctttgt tttcatatga aacgaattac ttgcttccct ttttattttt agtataataa
    31621 gagtcaaagg tcaggaatgg tcaatagaga tttcaccttt tctacctcat aaatctctta
    31681 aaaaactagg ggttattctt ttacctcatg aaaaacgaga tgtaaaatat ggatgtaccc
    31741 atttttgtga aaggacgtga atgattcatg ctttgtcaaa actgcggcaa gaatgaggca
    31801 acgattcact tgtatgccac tgtcaatggt cagcggacac aactcgatta ctgtcagagc
    31861 tgttatcaaa aaatcaaaaa tcaacaaaat ggaggtctga ctaatatggc ccaaaatgat
    31921 ccatttggat ttggcagttt ggacgatctt ttccgttcca tgtctcgcca aatgcaacag
    31981 caaggaaatt atgagcaaac accaccaaca caatttggcg gaaacaacaa cttcaatggt
    32041 ggtcagccac ctcaaggcaa tacagaaggt ttattgggag aatacggaat caatatcaca
    32101 gaacaagcac gacaaggtga tatcgatcct gtgatcggac gcgatgacga aatcaaacga
    32161 gtcattgaaa ttttaaatcg ccgaaccaag aacaatcctg tcttgattgg agaaccaggt
    32221 gttggtaaaa cagcagttgt tgaaggttta gcacaaaaaa tcgtagatgg tgatgtaccg
    32281 caaaaattaa tggacaaaga agtcattcgt ttagatgtcg tatctttagt ccaaggtact
    32341 ggtatacgag gacaatttga agaacgtatg caaaaattga ttgaagagat ccgtcaagca
    32401 gaaaatgtga tcctattcat agatgaagtt cacgaaatcg tcggtgctgg ttctgctggt
    32461 gatggaaata tggatgctgg taatatttta aaaccagctt tagctcgagg agaattgcaa
    32521 atggttggtg ctactacctt aaatgagtac cgcatcatcg aaaaagacgc tgctttagaa
    32581 cgtcgtatgc aacctgtacg ggtagatgaa ccaactgtcg aagaaacgat cagtattttg
    32641 aaaggtttgc aaaaacgtta tgaagactat catcatgtaa aatatactga tgaggccatc
    32701 gaagcagccg caactttatc caatcgttac gtccaagatc gcttcttgcc ggataaagcc
    32761 atcgatttgc tagatgaaac aggttctaag aaaaacttga cgatccaaat cgttgatcct
    32821 aaaacaatcg agaaaaaact gcaagaagca gaagaacaaa aagttttagc ttctcgagaa
    32881 gaagatttcg aaaaagcagc ttattatcgc gatcagatca ataaacttca aaaaatgaaa
    32941 gaacgtcagc tcactgaaga ggaaacacct gtgattaccg aaaaagatat ggaaaaaatc
    33001 gttgaacaac ggacaggtat tccagtgggt gaattaaaag aaaaagaaca gacacaactg
    33061 aaaaacttag cagatgactt gaaagctcat gtcatcggac aagataacgc tgtagatcga
    33121 gtagcgaaag cgattcgccg aaaccgtgtt ggattaaaca aacagaatcg tccaatcggc
    33181 tccttcttat ttgttggacc aactggtgta ggtaaaacag agcttgcgaa acaacttgcc
    33241 tacgaactat tcggttcaca agattcaatg attcgtttcg atatgtctga atatatggaa
    33301 aaacatagtg tctctaaatt gattggttct cctccaggtt atgtcggata cgaagaagct
    33361 ggtcaattaa cagaaaaagt acggagaaat ccttacagtc ttgtcttgtt agatgaagta
    33421 gaaaaagctc atccagatgt tttgcatatg ttcttgcaaa tcttagatga tggtcgattg
    33481 acagatgcac aaggaagaac ggtcagcttt aaagatacga tcatcattat gacaagtaat
    33541 gccggaactg gtaaagtaga agcaaacgtt ggttttggtg cggcacgcga aggtgttact
    33601 cgttctgtat tgaaccaatt aaacaactac tttacaccag aatttctgaa ccgttttgac
    33661 gggatcatcg aattcagtgc attatcaaaa gaaaacttga tgacaatcgt cacactaatg
    33721 ctagacgatg tcaaccaaat gctggcagca cagcaacttc acatcgaagt tcctacgaat
    33781 gtcaaagaaa aattagttga cttaggctat gatccatcta tgggtgctcg tcctcttcga
    33841 cgcacgatcc aagaacaaat cgaagatggt atcgctgaat tttatcttga tcacccttct
    33901 atccatgagt tgaaagcaaa actggataaa gatggaaaaa tcatcgttac ttctaaacca
    33961 gaacgtttgg ctaaagaatc agccgaagaa acggctgaat aaatccagtt ttatagcgtg
    34021 agacggtatt tctttgagaa atgccgtctc attctttttt cagaaaagtc aaaatactgt
    34081 tcattttata aaaaagaaat tataataaga gaaacaagca attaaaaata cataaaagag
    34141 gtgagcgcca tgaaattggt aaatgtaaca aacagccatt cccgtcttgt attaaaccag
    34201 cttgaaaaca ctgatgccca tctagtcaag gtctacacag ccggaaatac cactattgtc
    34261 tacactgaag cccctgaaca taatgaaatt ctgctgatca atgacaaacg taaaatccag
    34321 ccgaaagaaa tcgaagatgc taaaaattat ttcttagata aaatcaaaga cgccgtttac
    34381 cacgaggatg aaatcaaagt aatcgaattg gcaggccttg ttgaaatctc gattccaaaa
    34441 catgaattag gcgctacatt agccgtgtaa acaaattttg aacataataa ttaaagagcg
    34501 tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcgaaagc
    34561 agaagcaact ccttcggaaa taagctgaaa ttcacgaaaa ttgcttttca aatttttgtg
    34621 aattccagct tatttcttgg agttaaacgc ttctgtctca gcctcttttt ctttctagct
    34681 ttttgatttt tctcttttct tttgtacttt tctcttttca ttttcagttc tacgcgtgtc
    34741 tttattttta actttttcct ctttatggat gattgatcgt actttttgtg caatttgctc
    34801 gtttttgtcc cagtctttta catctaataa tcgacataat cgtaacatct cttctttttt
    34861 ctgttttttt ctttcacgtc tttgccattc tgtttccatg tgtcacactc tcctttcttt
    34921 aatcatatgt acgcaaaata atttttatat ttttttgatt agacatttct atgattttaa
    34981 tatagtaaaa agagccgtca cggaaaatcc gtcacggctc taaacaaaat atgagaggct
    35041 gtttttgttt ggctattctt attacttgat gcagggcagc ttgtgtcaag acttctttac
    35101 atcaaacttt ttaattttac attgggatat ttatccgcaa accagcgttc tgcaaattgg
    35161 ttctcgaata agaacaacgg ttggtcgaaa cggtcacgag ccaagatatt gcgacttgaa
    35221 ctcatcttct catccaaatc ttctggatcg atccagcgag cgattttgtg tcccattgga
    35281 gacatgatta cttcggcgtt atactcattt aacatacgat attggaatac ttcgaattgt
    35341 agctgaccaa cagctccaat gatatattct tccgttagat atgttttata aagctgaatc
    35401 gctccttctt gaactaattg atagattcct ttgtggaagg atttttgttt catgacattt
    35461 tttgcagtca ctttcataaa cagctctgga gtaaatgaag ggagttcttc ataagcaact
    35521 tttagttttc cttcgaacaa tgtatcccca atctgataat tgcctgtatc atatacaccg
    35581 atgatatcgc ctgctactgc tttttcgacg ttttctctcg aatctgccat aaattgtgtg
    35641 acattgctta atttcagttt tttatttgta cgttccaacc agacatccat tcctcgttcg
    35701 aatgttccag aacagattcg gacaaaagcg attcggtcac gatgggctgg attcatattt
    35761 gcttggatct tgaatacgaa acctgaaaat tcttcctcat aaggacttac catctgatca
    35821 tcttgtgttt tgtgtccata aggagctggt gccaattcaa cgaaagtctc taaaaaagtc
    35881 tgtacaccga agttagtcaa tgcagaacca aaaaatactg gtgtctgatc gccacgagca
    35941 attttttctt gattaaatgc atctccagcc tctttgatta gttctacttc ttctaagact
    36001 tgttcatata aggaattttc agttagagga tgattttcgg gaagcagtcc atcttcatcc
    36061 aattctacta atcgttcatc ctgataattt tccggacgat aaaactctac acgattatgg
    36121 tagatatcat aaagaccttc tagcccttta cccataccga ttggccaatt catcggataa
    36181 gattcgatat ctaatagctc ttctaattct tctaacaatt ccaatggttc acgaccatca
    36241 cgatctagtt tgttgataaa agtaaatatc gggatgccac gtttttttac aacttggaat
    36301 aatttctttg tctgtgcttc gatccctttt gcactatcga ttaccatgac cgcactatcc
    36361 acggccatca aagtacgata agtatcttca gaaaaatctt catgtcctgg tgtatccaag
    36421 atatttactc gtttcccttg ataatcaaac tgcataacag aactagtaac agaaatccca
    36481 cgttgtttct cgatttccat ccagtctgat ttggcaaaat tacctgtttt tttgcctttt
    36541 acggtcccgg cttgacggat agccccacca aacaatagta attgttctgt aatcgttgtt
    36601 ttcccagcat ccggatggga aatgatcgca aaagttctgc gattgtccac ttgttgcttt
    36661 aattttggat tattcataac ctaactccat ttcttccatt caattattta tattttctct
    36721 aggcacagat tgtccgatct cttaaagaaa gacaatttca tatagggctg tgacaaaagc
    36781 cgtgtagcag tctctttttc cgagtaaacg gcgttctaga gacagcctat cagcaataag
    36841 cccacccaag ccaaaaatac gaaaagctgt ttttgcttgg ctgctcttat tacttaatgc
    36901 aggatagctt tttcacaacc tttgtcaact actacaactg aatcagtata gcatggattc
    36961 agcgaatgaa aaagccacca aaccataaga gtcaaatatt tttttgtcag ctgactaaaa
    37021 gaaaagacgt gttgcttgtt tattcaagca acacgtcttt cataaccaag tttccaatta
    37081 tcttcttggc tcgtcatctt cgaattcttt tctgaagtat cttcgttctt cttcaggttc
    37141 cgcttctgtt tcttccggat catgaaaaat gacacggatt ttaaggattc gagaaccttc
    37201 catttcttca gaaaccagcg taatattatc gacatcaaac gataactttt cgccttcatc
    37261 cggaatcatg cctaatgctg taatcaaata accagccatc gtatctacat cactcatatg
    37321 cagatcactg tcaaaagctt cattgaactc atcaatcagc atacgtcctt ggatgatata
    37381 ttcgtgttca tcgatttttt cataaagatt ctcaacttca tctgattcat catcgatttc
    37441 tccgacgatt tcttctaaaa ggtcttctag tgttgctaac ccaacgacgc cgccatattc
    37501 atctaataaa atagccatct gattctgcgt ctttttcatt tcatataaca gatcatcaat
    37561 aaagaccgtt tcaggaacga ataaaggctc ctgcatgatt ttcttgatat ctaaattatc
    37621 aaagccgaat ttatgggcag cttttagtaa attctttgta tgaaggattc cgactacttt
    37681 atctttgtct tcgttataaa cagggattct tgaatagttt tcggataaaa cttcattcac
    37741 attttcttcc acagtgtcgt tgatatcaat cataaatgca tccgttcgtg ggaccatgac
    37801 ttctcgggca actttcgtat caagagaaaa aaccccttgg agcatttcta attcttcatt
    37861 attcaatacg ccttcgtttt caagcatata acgcatttca tctctggtca tttttgaatc
    37921 ttcatcatca aaagtcatcg gcgtgatacg cgccaataaa cttgtggaag cagaaagcag
    37981 ccagacgaag ggcttagcta ctacaccaat caagcgtatc agccctgaag tgaactttgc
    38041 gacttcttct gatttattta aagcaatacg tttagggtac aattctccaa aaacaatcga
    38101 tacataggtc aaaatagcca gcacaatgat atttgcaata ttttttgccg ctgtgccgcc
    38161 acctaatacc ggagctaacc gttccgataa agtattagct agtgaagcac cagacaaaat
    38221 gttgaccaaa gtgattccta cttggattgt cgataggaaa ttatttggat tctgcaatat
    38281 ttttaaaagc ttttgagatt tcacatctcc ttcatcagct ttttgctcga ttcgattctt
    38341 attgatcgaa acaacagcaa tttcggaagc tgctaaaaaa gcattgatca atgtcaaaac
    38401 gatcaataat aaaatctgcg ctaacagcga ctgactctca gggtcagcat tcataatcgc
    38461 attctctcct tataaaatta atttttagtt tacgatagaa aaaaacgtgg cactatagcc
    38521 acgttaaaat ggtatcacaa agcaattttc tttgcaataa aaacaagtgt actaaaacta
    38581 cctacgaggt cgtagcaatt ttcttcttgt tttcattttg cttgtcttct ttgacgattt
    38641 tgattatata ggaaatgatc gtcttgcaga ttgcgtaaaa aggaacacct aagaagatac
    38701 caagcaatcc ggcaatattt ccagctacta gaagaacgat aatgatcgtt aatggatgga
    38761 ttttcagtga tttcccaatt acattaggat aaatgatatt cccatccaac tgttggacga
    38821 ttagcacaac cacacaacaa agtaatgctt ttaccggatc attgaagact gttactagaa
    38881 atgcaggtgc tagtcctaga taaggtccta aatatggaat taggtttgtc aatcccgcaa
    38941 tgaccccaaa taagaaagca taatccacac caattaccat atatcctaaa aatgtgaagg
    39001 taccaacgaa taaacattcg attgcttgtc cgctaatgta attagccaat gttttattca
    39061 gttgccccaa taactccaca atttggtttc ttcttttttc tgggaaaaac tgttggatgt
    39121 ttggcactag tttttctccg tctttcaaca tatagaacaa gatgaatggt gctgtaatga
    39181 ttactacggt tgccgaagcg atcgttccaa cgatcgaacc taaactattg gatactccac
    39241 taaggaattg ctgaatgatt gtcccgtaag aaatgtctaa ttgctcaaaa tatttattga
    39301 tatctacttc cttgaacaaa ggcaattcgg ctacctcttt taaccagctt tctacctgtt
    39361 tgatgaaatc aggcatatta gatgccaatg aggaaatttg ctgtactaga cttgggatga
    39421 cactaagtaa aatccaaata attgctgcaa ctaaaaggag cagcacaatt gcgacagccc
    39481 atattctttt gatttttgct ttcatcaaca gcttgacaat aggattcaat aaatagtaaa
    39541 gaaagcctgc aatcagtaca ggtgcaaata acgtggaaaa aaatgtcccg atcggttcga
    39601 aaataaagtt tatttctgat gaaatcatga tcaatgcagc tatgatcaat aattcaagcg
    39661 accaaaacat tagtttagac tgtctaagtt tttcaaacaa tagatttcct cctttctttc
    39721 ttactagtat atattatatt cgttaaaaat tcttttattc tttttttatt tttgaaaact
    39781 tgccatactt tttatttgtt ttattttcca gtatactata cgaaaggaag gtggctagac
    39841 catgaaagtt gtacaaacaa aagatacaat gagcaatatt tacttagatg ctgtacggat
    39901 caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg tctcgagaaa tagacaaaga
    39961 tgaagcccat tgtatccact tcgtcctgta ctcagataaa aaagaaccgc aaggaactgt
    40021 acgtcttttg cctttagaaa acggaaaaat gaaattacag cggatggcga ttctttctga
    40081 atacagacac caagggttag gaaaaatatt gattgaagaa gcagaaaact ttgctaagaa
    40141 tcaaggctat aatacgattt tattaggggc acagtccaca gctgaaactt tttacgaaaa
    40201 actaggatat acagcttacg gcgatccttt tgaagatgct ggcatgcctc atatttatat
    40261 gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc ttttgagaaa
    40321 aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt gcttgtaacg
    40381 gttataccaa atatcaatat agccttccga gaacgggcca tctccattat tgatccagtc
    40441 aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcgggt aattcatcat
    40501 tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt caggaaatac
    40561 cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca aaaaaggtga
    40621 agctagatta caataatagg aaaccccctt ttctctgatc attgctatta cattgaacca
    40681 gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc gtccatcaga
    40741 ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg tttttaatac
    40801 catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt ggatcgtcac
    40861 aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat tttgtgcttt
    40921 gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa tgagctttgc
    40981 ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt tcatttggac
    41041 cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag ttttgttctc
    41101 ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa catgccattt
    41161 cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa caaagagttc
    41221 ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa acagatcgat
    41281 ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg tcacttccat
    41341 cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt caaaataata
    41401 cgctccagaa tgattttgaa gagctgcact gatataataa acgtcttttg gctttgcttt
    41461 ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac aaaaggcttg
    41521 gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac ctagatccat
    41581 cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca tcgcttcatc
    41641 aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca atctgctgac
    41701 tactcgcatc acgttgccgt caactgcagg ttctggaagt ccaaatgcga tacttgcaat
    41761 cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag gcgtttgcgg
    41821 catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt tccgggctct
    41881 tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag catttgctag
    41941 ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca ctgtgtctac
    42001 tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac gattatagcg
    42061 ccaaggcagg tttctttttt cttgttcata ccattgaatg aactgatctt gaaattcttt
    42121 tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc ttttccgtaa
    42181 tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc tttactttta
    42241 gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct ccttgtttga
    42301 ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagct atcgcttgtt
    42361 ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg ttggattttt ctcaataatt
    42421 ctttttggct ttttccgtga ttttttccaa acgctttttc ggctgtctga tgtgcatttt
    42481 gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca ggtttcaaac
    42541 ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag cgcatattcg
    42601 tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct tttaagcggg
    42661 ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat gttcggattt
    42721 ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa ccgaaatcag
    42781 ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt ccttttaata
    42841 atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca gataaatcta
    42901 cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata aacatcctcc
    42961 tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat tatatgttag
    43021 aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt gtcaaaactg
    43081 gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga atcggttatt
    43141 tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta ctagtgaaaa
    43201 tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa aaaacaagca
    43261 aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt caattacaac
    43321 acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa gcattggaaa
    43381 aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg atggatgagc
    43441 cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag actcaaaaaa
    43501 ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca gactgcttgg
    43561 ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg aataaatttg
    43621 atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc atggttagaa
    43681 tcggtgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa aaattcccgc
    43741 aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc cgaagttgtt tcaatcatgc
    43801 aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc cacttatggg
    43861 gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct cctcgtgcct
    43921 tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc aaagcattag
    43981 atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg atcggcttaa
    44041 gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa gcaatcgctg
    44101 atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa gtcgggacag
    44161 cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt atcatcgtag
    44221 atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa taccctccaa
    44281 aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg gttaatttaa
    44341 gtaaaaagtt caagattgaa tatttacagt ccgtcgacat gtttcctcag actgcacgtt
    44401 gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt gtgaaaaagt
    44461 ctgccctgta tcaagtaata agaacagcca aacaaaaata gctcttcatg ttttatgaat
    44521 actgtacttg tgaccgaaga gttaacagga ggacactatt tatttaaaaa aagggttgtg
    44581 acatagtttt tgtcacagcc ctttttgatt attattggat attcacgtct atcacttgat
    44641 aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa aatcacatta tatccttttc
    44701 gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg tggaacagtt gctttgtcgt
    44761 ctatttttgt gatcaattca aaatcactaa atttcaaagg ttgattagga ttccaagttg
    44821 gtttggtaat gtagtaatcc caagtacttg tccgatgttg tgctgttaaa ttccaagtaa
    44881 tatttaatgt tcctggatta accatgtttt tatgccatct gttagatgtc tgttcatcta
    44941 aaggctcaaa gccgctgact cctgcactag caatttttcc atcaatgaat gtatttttgg
    45001 tagcttcgat actttgcggt tcccattgtg ctcttccgac attttgattt aagtttcctg
    45061 ctgctgatgt tcctaggtat gcacggcttc ctggagaagt aatataacca tgcgcaaaac
    45121 ctggaatgct aaaaccgaac aaccctaaac taactaaccc aataaacaat aacgtactaa
    45181 tctttttcat gtagaaacct ccttcatttg ataaccacat tgtaaacgct ttattgtatt
    45241 aacacaaaca ttatgctttt ataattttat gttgaagtaa aaaaggtgaa aaaagtaaga
    45301 tgcagattat aacagaagta gacgagaaaa gtacaaatag aaaacatgag aatcaaatca
    45361 tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt tcaacttttt aataacaaca
    45421 attatcaatt tttttcttac ttcacaaact aaatacaggt cttgctgtga gccttcatgg
    45481 taaactaata ttatagaaag gagttagtaa aatgaagatg ataaaagata ttcgtttttg
    45541 ggtttgtgtt ataggaattg tcatattagg ctttttatca gggttgctta gcggaaatcc
    45601 cggaaaatat tactattctt tgcaacttcc accatttgca ccacctagct ggatatttgg
    45661 gccgatgtgg accttactgt atatattgat gggtatttct ctttatttgt tacttaatca
    45721 caacaacaaa aaacagcgaa acaatcttgt cggtcttttt gtgattcagt tcatttttaa
    45781 ttttatatgg tctgctctat tttttaattt aagaaatatt ttcattgcag caatcgatat
    45841 tacactttta gttattttct tgtcagtgct tatgtatcag ttgtggctgc atcatcgcct
    45901 ggcaatgtgg ctcatgattc cttattattt atgggtacta ttcgcaactc tattgaatta
    45961 ttcaatttat tttttaaact aagcgctaaa aagagacgtc ctgtaattta acacatagaa
    46021 aaagtgccac ctaagttaga ttttttgtct aatttaggtg gcactcttta ttaaccaaac
    46081 tatttttttc tagtacccac ttgttgttcc atactcatta ttctggtcat aaccataacc
    46141 attttgttcc tgtccagcgt aaggatcata actttgagta tcttgacttg gtgtatagta
    46201 ctgacttgta tcttctgttt gctgataata ggtattcgga tcttggtact gatcatatgt
    46261 gttttctgaa taggaagaat aatcggtgta cccttcacta ttcgatccat caccgtttgt
    46321 cgaatcgcca tcttccgaat catcaaagaa catcattgaa gcattctcat ttttcaaatt
    46381 aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc agttcatttt ggatcgaaag
    46441 aagatcattt tttcctaaaa tttgatagta gatatcattg atcatttgtc cttcgccttg
    46501 caactggtct tgtttgattg tttcaaacgc cggggtgtag ttcgtcgcta catcaagcat
    46561 gtcatcccaa tctaagtccg tcgtcacatt gttctcaact gcttttaaaa tttttctata
    46621 attgcttacg ccatctaaac ttaaaacttt attgacgatt ttcgtcacta cttcccgctg
    46681 acgacgttgt cgaccgatat cgccttctgg atcttcgtgt cgcattcgag aataggctaa
    46741 acctttttct ccattcaatg tttgttcacc tactggtaca tggacaccat ccaaagtaaa
    46801 gtcaatttta ttattcactt tgatgccacc gaccgcattg atcaaatctt ccatgccatc
    46861 caaattgatc gttacataat gatcaatagg aatatcgagc agttgctcaa ttgaatccat
    46921 ggccatttcg acaccaccaa atgcgtaggc atgatttaat ttatctaccg ttccataacc
    46981 tacgatattc gtataaatat ctcggtccaa actgatgatc gttgattttt tttgtttcgg
    47041 attaactgtt actaccatca tcgtatcaga acgtccttgt tccgttcttc ctaatccacc
    47101 ggtatctaaa cctaatagca aaaccgaaaa aggttctttg tcatcaatgc ttactctctc
    47161 agaacgtttg gaggatattc gattagagtt tttagaaatt ttgtttactg tttggctcgc
    47221 ttctcccata aatctgattc cgtatgctga taatccggca atcagcaaaa cgaatacccc
    47281 taacaaggtt aatagaactt tttgccatcg cttcataata cacctctgtt tcacattttc
    47341 taaaatattg tctcatataa ctgttcaatc agcaaattca ctttttaatc ataagaaaac
    47401 tttaaaaaac atttttgtgt aacgattatt attttttaaa atttctcaaa attcttaact
    47461 atcataccat accgtgaaaa agatcgaaag accgaataca cgcagttcgc aatcgtttta
    47521 tcttttttta tgtatttttt ccagaaaaag cgaacaacaa ggtattttcc aaataatcgt
    47581 gcagtaaaat gatcgcacat acctttccca tctttttaac agtctttgcc taccgttcag
    47641 attatcattc gatttcacga caatttcttc ctatgactgg tccttttgtt aaacccatct
    47701 tctattcgtt ttatttccag ctcactgcat ggctcttaaa atgactcgaa ctagtgattg
    47761 cattaccatc atcttttaat ggatagatat gctcctcttc aaagtaaaac gattttcttt
    47821 taaagtaata gaattgacat ggtaatgagt atgtatacta gtggtttcac aaatggtctt
    47881 ttattcaaca tccaaaaact gaaatttctt taaaatccat tactaacgaa gaaaatgttc
    47941 cttgcttgat aattagttag gacatgtcaa gtgaacaaat gcatttactt agccaaaaaa
    48001 tctaaaaact tcataaatag acaaaaagac acgactctct aagaagtcgt gtctttattc
    48061 aacaaattat tataatttag tcacttcagc agcttgaggt ccgcgtgcac cttcaacaat
    48121 cgtgaattct acttcttgac cttcttctag gctcttgaag ccgtctcctt gaattgctga
    48181 gaaatgaaca aatacgtcat ctccgccgtc tactgtaata aacccaaatc ctttttcatt
    48241 gttgaaccat ttaaccgttc cgtgttccat aaatgtatat cctccaaaaa agttcttgca
    48301 agtaaccttg caagaacatt atacccaatt tagaaaacgc tagcaagctt tcgctctttt
    48361 attttccaaa ttatttttaa gactgatatg acggaggaaa tggttatttt gctgcttcga
    48421 aacgtttgtt tacttcatcc caattaataa cattccagaa tgctgcaata taatctggtc
    48481 ggacattttt atatttcaaa taataagcat gttcccaaac gtccaaacct agaattggtg
    48541 tctttccttc catcaatgga gaatcttgat ttgcagtaga ggtaatagct aattttccat
    48601 tttccattac aagccaagcc catccagaac caaatcgtcc agcggctgct tttttgaatt
    48661 cttctttaaa agaagaaaaa tcaccaaaag cttcattaat cgcttctttt atttctcctg
    48721 taggttcgcc accagcattt ggtgccatga tttcccagaa aaatgaatgg ttagcatgtc
    48781 cgccaccatt gttacgtaca gctgtcttga tatctgttgg aatagcgtcc atatcagata
    48841 ataattcttc tattgttttt tcgcctaatt ctgggtattt ctcaattgct gaatttaaat
    48901 tcgtcacata agtattgtga tgcttatcat gatgcagatg catcgtttct tcgtcaatat
    48961 aaggttccaa tgcgtcatat gcataaggta gatctggtaa tgtgtaagtc ataaaaaaat
    49021 tcctcctcaa tgttttctta ataatgtcta cattacaaag agtaacaaag agaaggaaat
    49081 tcttcaattg ttatgcctag tcgtcttttt cactttgacc tacttcttct tcaaaaacct
    49141 cagtttcttt aaaaataaac aacttgctga aaatatagtt cgcaaggata acaataattt
    49201 gagtgatgat tttcgccaca tactcattgc tgttcataac attgatcaac aataccattg
    49261 cccccatgtc caatccgtaa gaaaggatac ggtagaagaa gaacttaccg cattcaataa
    49321 gtgcatgtgt aaaattcggt gattttgaat ggaataccca aagtttgttc gttacaaaag
    49381 caaacaatac agaacaaaac cacgaaatag aattggataa ggccatactt aaatcaaaat
    49441 attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt agccaaacca ccaaaaaaga
    49501 gatagactag tatctcccaa tatccttttg cttctaaata tccttttaac tgtcgatact
    49561 gcttcatgag ccaacctacc ttattaaaaa ttcttattta tcatactcta tatttcaata
    49621 actttcatct ttatttaaaa aaattaattt tttttgatcc tttgcctttg tctctgcttg
    49681 gatcgtcaca tgtgtaatac catattttaa ttttaataaa gaaattatac gaggatatat
    49741 cttttcaatc tcgcttagta gctgatcttc aaaatcaata tgtccgtcga aaataattat
    49801 cttttcgcta tactgccaaa tatggacatg atgaacatcc tttaccccat cgattgcttc
    49861 aatttcttgt tccatttttt catagtcgat agcaggtgca ctttgtatca aaatagcggc
    49921 agcttttttg atcaccatta tcgcttctat caatatatat atcgaaatga tcagtgtaat
    49981 aactggatcg atccagtaga tatcgaaaaa acggatcaaa attgctccga taatcacact
    50041 taccgaagac agtgtatcac ttaacaaatg caaataacta gattttatat tcaaactttc
    50101 atctgcttct tgtcgtaata ataatactga gaacaaattc gcgagcaatc caataactgc
    50161 tactgttagc atcaaatgac tatttatctt ttctggtgaa gtgaaccgct tgaatgcttc
    50221 aacggttaaa aacaaggata atatgatcaa tatgaatgag ttgatgaaag cactcaatat
    50281 ttctaaacgc tggtagccat acgtacgttt tttatttgct tcttttcggg ccattttttg
    50341 tgccccgtaa gaaaaaataa ttgccaatgt atcactaagg ttatggaaag catctgaaac
    50401 aagagacaaa ctccctgaca taatccctcc aataaattct gataaagtga tcaccgcatt
    50461 caaaaccact actccaaata gtttttttga tgatctctgt ggctgtgtag gcactattcc
    50521 ttcctccatt ttatttatat tggatagtct ttagacaaga ataacatttt tttgatattt
    50581 caccaatcat tcagctgttt ccttaaagca cttgtatttc acttgatact tcgctagaat
    50641 tgcaagtgat gacactacta gatgaggagt acttatgaca acaaaacaat taattatcag
    50701 ttcctttata cgattcaaag acctaaagga ggcgagaaat gtcccatttt ggaaagccat
    50761 tgtctatctt ttagttttga gtataattat ggctttgcca atctcttttc aagtttttca
    50821 agtcttaaac aacattcaat tggatgggca aaagattgct actcgcattc ctgattttac
    50881 aatccaaaac gggcagcttc aaaccgaaga aaaagaagga ttcatttacc aaacaaactc
    50941 cattatcttc acatttgatc cagagggaaa acgaacagaa caggacatct ctactgattt
    51001 gatggggaat tttgttagtg tcgggatgtt aaaaaacaaa cttatcatag ctcttcctaa
    51061 tacaggcacg acttctgctt tgttgaacaa taaccaatta gaacttccct acaccaatga
    51121 atctttaaaa aacttaacag gtaagcagct ccgttctttt ttaagtgaag catctatacc
    51181 actttggata aaagtcctga catttatatt ttctatttat cctagttttc tgaatctgct
    51241 gatcactttg ctatttacca acattgctgc ttttctctat gcacgctttc gactaacaaa
    51301 agctactttt cttgattgtt taaaaacatt gatctattca atttcattgc ctactataat
    51361 tgcaacaatc ttgatggtct ttcttccttc ttttgacact agcgccttca ttgcttttgc
    51421 aggaatcttt atttttgctc aagctgtcaa aggatgggca aaaatatcga tcaattaaga
    51481 aataaagaca actcgttatt tctacttcaa tagacccttt tagaggttgt gacatttttt
    51541 tgtcacagcc ccttttttat ttgaaaaata tttctttctc aaaaaacaag caaggcgctt
    51601 caaagttctt cttgtatgcg ctacacttag agtaaagaaa ggcgggaaat taactatgtc
    51661 agaaagaatc atttatgcag atcgtccatt tcgtgatgaa tttatcgaag aaatccatac
    51721 tatagcgcct ggttatatct ttaaaacaga attacaacca gaggatctac aaaatgtaga
    51781 gatcagtttg ggctggaact cagactacca agaaaaacta ttagcctctt ctaccttgaa
    51841 atgggtccaa tctatctcag ctggtgttga tcagctaccc cttgagactt tttcaaagaa
    51901 acatatttta ctttcaaatg gtagtggcat ccacagtgaa tcgattacgg agcatgtctt
    51961 aggaatcatc cttgggtatt ctcgtggtct ttttcaagca caaaaggcac agagtgacat
    52021 gaaatggtta ggttcatcgg tccattacca atctatccaa gggaaaaaaa tactaatcat
    52081 tggaactggc catattggaa aaatgattgc aaaaaaatgc aaagcattag acatgaactg
    52141 ctatggaatc aatacatccg gtcatccagc agaaggaatg gaaaagactt tttctttaaa
    52201 tcaactaaag gaagttcttc ctgaagtaga tttcgtagtc aatatcttac ctctaacgga
    52261 ggaaacaact gggctctttg atcagcatat gtttgaacac tttgatccaa aatctgtatt
    52321 catcaatgtt ggccgtggtg cttctgttaa gacacaagat ctggtgcagg ctttaaataa
    52381 ccagcaactt tcctttgcag cattagacgt ttttgaggaa gagcctcttc cacaagatca
    52441 tcctctttgg aaaatggaca atgtactgat tacttcccac attgcaggtt tgacacctga
    52501 ttttcaaaag aaattaatgg ctattttttt aaccaatctg aaaagctact ttgccaccca
    52561 tgagcttcaa accaatcaag taaaattgtc tgcaggatat taatagctga aaacaatatt
    52621 ttcaaaagaa aaaagagaag gtgacaaaaa ttgtgtcacc ctctcttttt ttattgtttt
    52681 ttaaagtgat aggttggctc ctccaggttg ggatcataat caacaagtaa atcatatcct
    52741 ttaaagaacc attcatctgc ttcctcgata aaaaaaagtg tcccatcaat ttccactttc
    52801 tttacaggat gttctggctg ttcaactgac attccaatcg aaaaaccatc atgaacctct
    52861 gttttgccgt agacttttcc aaaaaaacgt attccgtaag tttctggcag atcaatttct
    52921 gagtcaaacc acttttgtgc ttttggcgtt accgttaatt tcatcccaac aacctacttt
    52981 ctcttttctt tctattgatt catcttatcg tctagaatat ccacatcgcc tgtctctcca
    53041 ataaccagta gattatcatt ttcacgaaca tattcatctg ctgcaggaga agcgataacc
    53101 gtttgatcta ctcttcgtat ggcgactact gttaagtcaa aacgttgcct gaaatttaat
    53161 tcagctaatg ttttgttgaa aaatttagga ttggagactc ggatttctgc taaagaatat
    53221 ttatctgaca actctaggta atctaaaata ttctttgaca ctaaattatg cgcaatcctt
    53281 attcccatgt ctcgctcggg atggacgacg cgatcagctc cgatcttttc aagtactctg
    53341 gcatgatact cattttgggc ttttgctaaa atatttggta cgcccatttc ctttaccatc
    53401 agagtaacta agatactggc ttgaatatct tctccaatag cgacaattac atgatcgaaa
    53461 tttcttatgc ctaatgatcg aagtgtcatt tcatcctgtg catttgccac aacagcatga
    53521 gtggctatat tcatatattc attgacccga tcttcattac tatcaatagc cagtacttct
    53581 tgaccagctt cgatcagtgt tcggcagata cttccgccaa atcgtccaag acctacaatt
    53641 gcaaaatttt gtttcaccgt tttcctcctt tagtatcttc ttttctcagt ataacaaagc
    53701 ctttacaaaa acaaaagcaa gcatcattat tgtaaaaaag ccaagaaaaa taatagttat
    53761 tttctcagct tttgattatc ctctttatca tcgtttttat ttcaccaaac catgtttgaa
    53821 tgcatagata gcggcttgtg tacggtcttc cacttctagc ttagccaata tattggaaac
    53881 atgtgttttt actgttttca gtgtaataaa caattcgtcc gctatttcct gattactttt
    53941 tccttcagaa atcagcatta aaatttcatt ttcccgattc gttagttctt catgtaatac
    54001 aggctcattt cgccgactca ttttttccat catttttgtt gtcacttctg gttctaatac
    54061 tcgctcgcca cgttgcgttg cccttatagc atcagcaatt tcatgtgcgg tagaggtctt
    54121 caacaaatac cctgctgcac ctgcttcaat ggcaggataa actttttcgt catcaataaa
    54181 actggttacg ataatgattt tagcttctgg ccaatctttt aagattgctt ttgttgattc
    54241 gataccatcc atttcttcca tgactaagtc cattaaaatg acgtccggtc gaagtgtcat
    54301 cgccttctca tagccttgtc gtccattttc agcttctcca attacttcaa tgtcttcttg
    54361 aatagataaa taaaaagaga cgcctaagcg caccatttca tggtcatcta ctaataaaac
    54421 ttttatcatc cttatctatg cctccttcat caaagggacc ttgatttcta cacttgtacc
    54481 ttgaccttta aaactaatga tttttactgt tccaccaata ccagctactc gttctttgat
    54541 attgttcaat ccataacttc ccgttttcgt ttcattcata ttaaaccctg ttccgtcgtc
    54601 aataatacgt aacaaaagat tattgtctat tttgtgcaaa tatacctcta attcattggc
    54661 tttagcatgt cttaatgtat ttgataacaa ttcttgaacg attcggaaca agtgatcctc
    54721 aatggaacta gatagtttta catcttctac atcccatttc agcgaaatct ggattttatt
    54781 ttgcaattcc ttcaataact gctcgatacc ttgctttaaa cttttctctt ctaaattgac
    54841 tggacgtaga tgaagaagca gcgcacgcat ctcagactgg gaagcgttga tgatctctgc
    54901 taccattttc aactgcttgc gaaacatttc tggtgtctcg cttttttctg cttgctccgt
    54961 caatgccgac atcatcatca ttgctgcaaa caattgttgc gaaactgaat catgcagctc
    55021 acgggctaat cgatgtcttt ctaattctaa aatttcttct ttggtttgtc catctacata
    55081 ttggggacgg ctcgttacaa tttgtaattc actagatact tcaatcattt tttcttttat
    55141 cttagtgatt tccttatcaa tatctttgat gtataaatcg tccgatgcac ttgggatcgg
    55201 aagaagcaac aatttggatt cataattgcc agaagaaagt gcacgcagct tttcttcgat
    55261 tttcccgtat tttgtctttt gaataagtga taataataga aagacaatta atcctacacc
    55321 taaggaaatg gctaacaaat ggaagataag cggaacatat aataaacgtg cacgcagtaa
    55381 ttctccccac caatgacttt gattgctggc ataaaaataa gtgaaaagtg aaaatagaat
    55441 aataaggaaa gccgcaatcc ccgaataaac agctagcatc gctctggata tttttcccat
    55501 catattcgta tcacctcgac atctccaatc aaggtattag taataatctt caatctacga
    55561 gggttttcat cataatcact gctgtagatt ttgatttgtt catttttcaa agaaaattgc
    55621 tcttcttcaa acaagacatt tcctactaat gtcgcatgct ctaatcggat ggctacgccc
    55681 aaaggtacta agatccgtgt tctgcctatc ccttttctta caatcacaat attatcatct
    55741 tttggcaaaa gcgtattgcc caaatctatg atagtatcac cagaaatgac ggcgatgttg
    55801 atatcgtccc attcatagac gtcgtttcca atacgttggt taccgaataa ttgctgtctt
    55861 ttccgctcat tattatgtgt ttttaattgc tctgtttgga tcatcatgat ctgtttcttt
    55921 cgccaaaaag catttttggt taaatctatc cctgaaatct ctacaccttt cagaccaata
    55981 aataaaacgg caaatacagc catcatccat aaagcaggac tgttgactaa gctaaaaaaa
    56041 atgaccaaac ttcctaatat caattgaaaa ttttgaaatt tagtacgagg atgtttgcgc
    56101 atagctagat atatattgaa aataccgaat atcactaaaa gcaaaagtcc agtattgttt
    56161 acgatttgcc aaacagcaaa taacagcagt agtgcttcaa ctaccacaaa aaaacgccaa
    56221 gaactattca tcgctctttc tccctcctga cacattctca tttcatcata cctatttttt
    56281 taactgttgg ctataggact ttagatgtaa gatcgtttgt tgaaatctaa aatacacaaa
    56341 gagaaaaaga tctagagtgt atcatcagta agatgaactc tctagatctt tcagtcagac
    56401 tacgcttgtt ctactttgac gattttaact aacatatcgc cacctggagt agcaatagtt
    56461 acttcatcat ttttacgttt gccaatcaat gcttgcgcaa taggagaatc attggaaatt
    56521 ttgccagaaa acggatctgc ttcagcgctt ccgacaatcg tgtactcttc ttcttctcca
    56581 tctggtagtt cgatgaatgt aactgtttta ccaattgata cctcatctgc atctacaccg
    56641 tcgttatcga tgatttgagc aaaacgaatc atattttcaa ttgttgtgat tcttccttca
    56701 acaaatgctt gttcatcttt cgctgattca tattcagagt tttcggaaag atctccgaaa
    56761 ccgcgggcaa ttttgattcg ttcaatgatt tctccgcgtt tgactgtttt tagttcttct
    56821 aattcttttt ctaatttttc tttaccttct agagtcattg gataaacttt ctctaccatg
    56881 tgtaacgtct ccttttttcg ataaaatacc gcaaaaggta atcttttgag gaatatagtc
    56941 caaaaaagat taccacgctt ttcttcaaat gtaaatgttt ttctaaaaat gatcaatgtc
    57001 tagagcctgt ttttctaaga attatttaca tatttttcca ctaattccat atgttcttca
    57061 taagttttag cgaaataaac atttccagtg ctcgtatctg ctacgaaata ataatagtca
    57121 ttttctgctg gttctaatac tgccttgatt gcctcttcgc ttggattgtc aaaaggtcca
    57181 ggtccatagc cggtattggt atacaaatta tatggtgaat ctacagctgt atcttcatac
    57241 gtcactaatt ctttgtgttc acctaatgcg tataaaacag agatatctga ctgaagtggc
    57301 atatttgctt tgattcggtt aaagaagact tgtgcaatgt ttttacgatc attttctttt
    57361 acgccttctt tttcaactag agaagctaga gttaaaactt cttgtacagt caaatttttc
    57421 tgcttgatag ctgaataata gttttgcatg accgtattcg tcttgttcac catttcaatc
    57481 actagatcct taagcgaagt actcgtgtaa taatcatatg tggctgggaa taagtaacct
    57541 tccaaacggt attttgtgtt ctcagcttta gatgcacttt ccaacagttc tgggaatttt
    57601 tggtgcaact cgttaaagaa agtctcatca ttcactaaat ctaaaaattc tttcttgtct
    57661 ttaccagtta ccttcgctac ccgttcagca atctgatcga tatcgtaccc ttctggaatg
    57721 gcaattttcc catctgcaac ttttgttggc tcagaagtac cgccttcttg caactgtttg
    57781 ccaatctcgt caagagtcat attaggcgct aactgataat agccagcttg aaagccagtt
    57841 aaatttttaa attttgtata ataattgaaa acaatcccgc ttttaataat attgtccttc
    57901 tccaaaatct caccaatctg cttgttagaa gagccactcg ggatctcgac ttggactaat
    57961 tggtcatctg atttatctaa aggcttcaac ccgctatcta cataacgata cacggtaaat
    58021 ccaagaaatc cgccaataat caataatgta agagcaatca ctaaaatgat cttcctcaca
    58081 atcttatctt cttttttacg cagatttctc cgcttgtcga ttatatctgc tgtagctgcc
    58141 ttactggctt cttttttatc tttacttggg tgttgtttgt ttcctgacaa aacaactcct
    58201 ccttactatc aagttcgtaa ctttattcat tatacatgat atttgaattg gcgtgaatga
    58261 ttttatttaa atattctatc aaaaatgtaa gtagtaaatc cattactata agattaaaat
    58321 aaatgttatc aagatacaag ctattttagc tttattcttt ctgtaaaaaa gaaaggagac
    58381 taaataaact attatgaaaa aaggaaaaca tgctaaaaaa agaaaatatt catttttaat
    58441 gatcgttgtc gtgttgttta gccttagtgc gttctctaca ggaactattc ttggatggca
    58501 attttacaat aatgaacact ctcagatagt tgcccatgct caatcagctc aaaaaaagtt
    58561 gatactgact accaatgaac aacagctcgt tgaaaaaatc gatcaactat taagaaaaaa
    58621 tgattatatt ggctcaattt acgtaaagaa aaataatcgc atcatcatgg aaaaaggcta
    58681 tggctatgcg aataaactca cgcaacaacc aaatgatcct tctctttatt atcagattgg
    58741 ttcggttcaa aaagcattga ctactctttt gattttaaaa caagtcgagc gtgggcacct
    58801 ttctttagat actaagctgg ctcaattcta tccacaaatt gctggcagcc agcaaattac
    58861 tatccgtgac ttgctgtata tgcgctcagg cttgcaccga actgcttctc caaccatacc
    58921 gatgactgat gaagaagtcg ttcaatttgc gattcagcac ttaaaattta cagattatca
    58981 gacctctcat tatgattcat tgaactttac catattgact ggaattttga tcaaattaac
    59041 aaatcaaccg tatgaaaaat tattgaccga tgatatcatc gaacctttag gattacagca
    59101 tacaaaattt tatgaccaag tcaaaaattc atccaatcat gcactatcct atcaaatgtc
    59161 taaagaggat gattacttta aaccactgac agaatctgaa acagagatcc ataacgaatt
    59221 agggactggt aatatcagta tgtccgtcta tgatttaaat acgctgttta cacaagtttt
    59281 atctggaaaa ctagtttcca aagacttact cttctcatta tggcgtgaaa atgcgaatgg
    59341 acatccatat agtggtgggg tatatagcgg agataattat atcctgtcgc aaggaaatat
    59401 cgactctttt gatacatctg ctatatttag caaagattca caggatgcta tcatcatgga
    59461 aagcaatata aaagctgaca aaactatcaa actgcctact gctgatctaa gaaaccaaat
    59521 ctatgacctg atggaaggaa ctactaagtt ggcaaatagc tgatatgatg aaaagatagc
    59581 aaatactact tctttgaata gcatttgctt aaaaaaagta ctcgactaaa cgtaggaaag
    59641 agtgtgggac aaatctaaaa atcaattttt gctacagact ttaattccaa ataaacagaa
    59701 cagaaagagg tgttcttctt tctgttctgt ccaaattatt ctaagatttc tctttaccaa
    59761 cattatttga caaagggcct attaaatcta aaatagtatc tttagattgc taaaatatgt
    59821 gctagcaaat cattcatggc ttgttcttat ttttaatttt atgcaataaa aaagaagaac
    59881 aattagcgtg attatagaaa taaataatga gattttagaa aaaaatgtgt atgaatacct
    59941 gattgttact ttatttattc cactttcact ttttactgta acaaaaggac ctccttgttt
    60001 tagtactgta gcattttttg aattaacagt aaccgttaat cctttatacc ggaatacagg
    60061 taaaactatt gttttgcctt ttgaagattt aaacgtaaat gtaatttcat ctttatttgg
    60121 agaaaagcta ctataaataa cttgaccatc ttctgtaact tgatggtttt tcaaaagcaa
    60181 ttgatctttt ttatagccta taggtacata atcaaaattc tcaaattctt caagtttatt
    60241 ttccatttgt tcattagaat aagttccttg aaaattcatt tctttcacca tgttaactac
    60301 tg
//
LOCUS       DAZKOR010000005        60200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000005 DAZKOR010000000
VERSION     DAZKOR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.005"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..1076)
                     /locus_tag="ACG8ZM_000234"
     CDS             complement(33..1076)
                     /locus_tag="ACG8ZM_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0491232.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1919)
                     /locus_tag="ACG8ZM_000235"
                     /pseudo
     CDS             complement(1237..1919)
                     /locus_tag="ACG8ZM_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
     gene            2114..3043
                     /locus_tag="ACG8ZM_000236"
     CDS             2114..3043
                     /locus_tag="ACG8ZM_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0491233.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3104..3895
                     /gene="phnC"
                     /locus_tag="ACG8ZM_000237"
     CDS             3104..3895
                     /gene="phnC"
                     /locus_tag="ACG8ZM_000237"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491234.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3867..4646
                     /gene="phnE"
                     /locus_tag="ACG8ZM_000238"
     CDS             3867..4646
                     /gene="phnE"
                     /locus_tag="ACG8ZM_000238"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0491235.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4643..5425
                     /gene="phnE"
                     /locus_tag="ACG8ZM_000239"
     CDS             4643..5425
                     /gene="phnE"
                     /locus_tag="ACG8ZM_000239"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0491236.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5428..6231
                     /locus_tag="ACG8ZM_000240"
     CDS             5428..6231
                     /locus_tag="ACG8ZM_000240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0491237.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6315..7862
                     /locus_tag="ACG8ZM_000241"
     CDS             6315..7862
                     /locus_tag="ACG8ZM_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0491238.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8159..8356)
                     /locus_tag="ACG8ZM_000242"
     CDS             complement(8159..8356)
                     /locus_tag="ACG8ZM_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491239.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8432..8632)
                     /locus_tag="ACG8ZM_000243"
     CDS             complement(8432..8632)
                     /locus_tag="ACG8ZM_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0491240.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8752..9939)
                     /locus_tag="ACG8ZM_000244"
                     /pseudo
     CDS             complement(8752..9939)
                     /locus_tag="ACG8ZM_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9984..11110)
                     /locus_tag="ACG8ZM_000245"
     CDS             complement(join(9984..10882,10882..11110))
                     /locus_tag="ACG8ZM_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0491241.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(11514..12296)
                     /locus_tag="ACG8ZM_000246"
     CDS             complement(11514..12296)
                     /locus_tag="ACG8ZM_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491242.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(12361..13197)
                     /locus_tag="ACG8ZM_000247"
     CDS             complement(12361..13197)
                     /locus_tag="ACG8ZM_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0491243.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(13215..14624)
                     /locus_tag="ACG8ZM_000248"
     CDS             complement(13215..14624)
                     /locus_tag="ACG8ZM_000248"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0491244.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(14638..16461)
                     /locus_tag="ACG8ZM_000249"
     CDS             complement(14638..16461)
                     /locus_tag="ACG8ZM_000249"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0491245.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(17304..17630)
                     /locus_tag="ACG8ZM_000250"
     CDS             complement(17304..17630)
                     /locus_tag="ACG8ZM_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491246.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(17676..18038)
                     /locus_tag="ACG8ZM_000251"
     CDS             complement(17676..18038)
                     /locus_tag="ACG8ZM_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0491247.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18075..18289)
                     /locus_tag="ACG8ZM_000252"
                     /pseudo
     CDS             complement(18075..18289)
                     /locus_tag="ACG8ZM_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            18417..18752
                     /locus_tag="ACG8ZM_000253"
     CDS             18417..18752
                     /locus_tag="ACG8ZM_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0491248.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(18828..20825)
                     /locus_tag="ACG8ZM_000254"
     CDS             complement(18828..20825)
                     /locus_tag="ACG8ZM_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491249.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(20923..21198)
                     /locus_tag="ACG8ZM_000255"
     CDS             complement(20923..21198)
                     /locus_tag="ACG8ZM_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0491250.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            21430..22164
                     /locus_tag="ACG8ZM_000256"
     CDS             21430..22164
                     /locus_tag="ACG8ZM_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0491251.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            22203..23345
                     /locus_tag="ACG8ZM_000257"
     CDS             22203..23345
                     /locus_tag="ACG8ZM_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0491252.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(23440..24831)
                     /gene="sufB"
                     /locus_tag="ACG8ZM_000258"
     CDS             complement(23440..24831)
                     /gene="sufB"
                     /locus_tag="ACG8ZM_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0491253.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(24836..25306)
                     /gene="sufU"
                     /locus_tag="ACG8ZM_000259"
     CDS             complement(24836..25306)
                     /gene="sufU"
                     /locus_tag="ACG8ZM_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0491254.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(25293..26528)
                     /locus_tag="ACG8ZM_000260"
     CDS             complement(25293..26528)
                     /locus_tag="ACG8ZM_000260"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0491255.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(26525..27811)
                     /gene="sufD"
                     /locus_tag="ACG8ZM_000261"
     CDS             complement(26525..27811)
                     /gene="sufD"
                     /locus_tag="ACG8ZM_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0491256.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(27824..28597)
                     /gene="sufC"
                     /locus_tag="ACG8ZM_000262"
     CDS             complement(27824..28597)
                     /gene="sufC"
                     /locus_tag="ACG8ZM_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0491257.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(28794..29642)
                     /locus_tag="ACG8ZM_000263"
     CDS             complement(28794..29642)
                     /locus_tag="ACG8ZM_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0491258.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(29666..30352)
                     /locus_tag="ACG8ZM_000264"
     CDS             complement(29666..30352)
                     /locus_tag="ACG8ZM_000264"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0491259.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(30345..31379)
                     /locus_tag="ACG8ZM_000265"
     CDS             complement(30345..31379)
                     /locus_tag="ACG8ZM_000265"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491260.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(31442..31698)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(31774..32112)
                     /locus_tag="ACG8ZM_000266"
     CDS             complement(31774..32112)
                     /locus_tag="ACG8ZM_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0491261.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(32114..32470)
                     /locus_tag="ACG8ZM_000267"
     CDS             complement(32114..32470)
                     /locus_tag="ACG8ZM_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0491262.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(32476..33663)
                     /locus_tag="ACG8ZM_000268"
     CDS             complement(32476..33663)
                     /locus_tag="ACG8ZM_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0491263.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(33854..34699)
                     /locus_tag="ACG8ZM_000269"
     CDS             complement(33854..34699)
                     /locus_tag="ACG8ZM_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0491264.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            34933..35898
                     /locus_tag="ACG8ZM_000270"
     CDS             34933..35898
                     /locus_tag="ACG8ZM_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0491265.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            36275..37318
                     /locus_tag="ACG8ZM_000271"
     CDS             36275..37318
                     /locus_tag="ACG8ZM_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0491266.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(37380..37844)
                     /locus_tag="ACG8ZM_000272"
     CDS             complement(37380..37844)
                     /locus_tag="ACG8ZM_000272"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0491267.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            38090..39037
                     /locus_tag="ACG8ZM_000273"
     CDS             38090..39037
                     /locus_tag="ACG8ZM_000273"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0491268.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(39248..41320)
                     /locus_tag="ACG8ZM_000274"
     CDS             complement(39248..41320)
                     /locus_tag="ACG8ZM_000274"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0491269.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(41253..41630)
                     /locus_tag="ACG8ZM_000275"
     CDS             complement(41253..41630)
                     /locus_tag="ACG8ZM_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0491270.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(41759..42319)
                     /locus_tag="ACG8ZM_000276"
     CDS             complement(41759..42319)
                     /locus_tag="ACG8ZM_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0491271.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            42469..43761
                     /locus_tag="ACG8ZM_000277"
     CDS             42469..43761
                     /locus_tag="ACG8ZM_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0491272.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(43821..44939)
                     /locus_tag="ACG8ZM_000278"
     CDS             complement(43821..44939)
                     /locus_tag="ACG8ZM_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0491273.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(44953..46374)
                     /gene="arcD"
                     /locus_tag="ACG8ZM_000279"
     CDS             complement(44953..46374)
                     /gene="arcD"
                     /locus_tag="ACG8ZM_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0491274.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(46620..47741)
                     /gene="mnmA"
                     /locus_tag="ACG8ZM_000280"
     CDS             complement(46620..47741)
                     /gene="mnmA"
                     /locus_tag="ACG8ZM_000280"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0491275.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            48145..48744
                     /locus_tag="ACG8ZM_000281"
     CDS             48145..48744
                     /locus_tag="ACG8ZM_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0491276.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(48882..50168)
                     /gene="efmA"
                     /locus_tag="ACG8ZM_000282"
     CDS             complement(48882..50168)
                     /gene="efmA"
                     /locus_tag="ACG8ZM_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0491277.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(50439..50783)
                     /locus_tag="ACG8ZM_000283"
     CDS             complement(50439..50783)
                     /locus_tag="ACG8ZM_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0491278.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(50920..52065)
                     /locus_tag="ACG8ZM_000284"
     CDS             complement(50920..52065)
                     /locus_tag="ACG8ZM_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0491279.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(52245..53219)
                     /locus_tag="ACG8ZM_000285"
     CDS             complement(52245..53219)
                     /locus_tag="ACG8ZM_000285"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0491280.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(53299..54174)
                     /locus_tag="ACG8ZM_000286"
     CDS             complement(53299..54174)
                     /locus_tag="ACG8ZM_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0491281.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            54304..56211
                     /locus_tag="ACG8ZM_000287"
     CDS             54304..56211
                     /locus_tag="ACG8ZM_000287"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0491282.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(56752..60030)
                     /locus_tag="ACG8ZM_000288"
     CDS             complement(56752..60030)
                     /locus_tag="ACG8ZM_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0491283.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT        17323 a        12493 c        10886 g        19498 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc agcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gtcagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaag taaagaagcc attgccaata tatctgaatc catataatcc
     1861 tgcaggttgt gcttctcaac gtaagcagtt aattgcttcg tatcatggat ttttttcatg
     1921 aaatcgtcac ctctaagaag tttttcttta ttatagctta gattgttttt cggctcaata
     1981 cgaataatta tttataattg tttgtaaatt gtatgtaaaa gttgtgtaaa ataggacgtg
     2041 cgtcacttca ttgattttga ttccctgcta tgatttagac gtattagaaa ttatatccaa
     2101 caggaggaga aaaatgcagt tcaaacagtt agcaaaaggg ttcatcggag tcatgttatt
     2161 gaccactttg gcggcctgcg gttctgggaa tacaagtagc agctcagaaa aagagagcaa
     2221 agaaaaatta gttgttcaat tcgttccaac aaacaatgat ggtagtatgg aggcaaaagc
     2281 aaaaccattt gctaagtatt tatcgaaaaa gttagatcgt gaggtagaag tgacgttagc
     2341 tactgattat tcaacgatcg tcgaagcgat gtcttctggt caagtcgatg tcggaattat
     2401 gccgcctgca gcctatgttc aagcaaagga tgctggagct gcagatgcaa tcctgacttc
     2461 tcagctcggc gattatgatc aagaaactgg cttgccatta aaggacaaac ttacgaacac
     2521 ctttaaagga gaagtattgg ttcgtgccga tagcggattg aataagttaa ctgatttaaa
     2581 aggaaaaaat atcgcaacct taagtcctaa ctctgctagc ggctacatct atcctgtcgc
     2641 tgaattgaaa gatgctggga ttgatcctac tgcagataca aagatgacca ctgtgaatga
     2701 tattccgagt gagattaccg cagtgttgaa caagcaaatg gatgcggcgt ttgtttttga
     2761 aggcgcgcga aatgtttttg cttcgaattt cccagataat gatttattta aggatttaaa
     2821 agtcctgtac ctgacaaaag gcgatattcc gaatgacgca atcgctgtac agcctgacat
     2881 gtcaaaagat ttaaaagcga aaattaaaga gaccttctta aatatgaagg atgataaaga
     2941 tggcaaagaa gcaatggctt tgtgggggca tcagggctat gaagaagctg ctgattctgc
     3001 ctatgacacg atcagagact acacgaaaaa agcagcagaa taaaacatca gtaagtgtca
     3061 gcaagctttt ttttgcttac acttaaaaaa atggagaagg ataatgatta tttttgacaa
     3121 tgtttcgaag gtctatccca atggcgttgt tggcttgaag gatattgact taacaattga
     3181 agatggagaa tttgtttcca tcatagggtt gagtggtgct ggaaagagca ctctgctgcg
     3241 cagtatcaat cgcttggcag aaaccaccaa aggagatatt cgcattaatg gaacctctat
     3301 tacaaaagcg aataaaaaag agctgcgaca gattcgccgc cagattggtc taatctcgca
     3361 aagcttcaat ctggtaaagc gtagtacggt tcagaaaaat gtgctatctg gacgtttggg
     3421 ctattactcc acttggaaaa gtattttagg tctttttacg aaggaagact accagcagac
     3481 aaaggaagca ctggaaaaag ttggtttgtc tgataaattg cagacacgca gtgatgagct
     3541 cagtggtggg caacaacagc gagtatccat tgcgcgagca ctcgtgcagc aagcccccat
     3601 tattttagcg gatgaacccg ttgcatcatt agatccaatc acgactcaaa aggttatgat
     3661 ggacctaaaa aatatcaatc aaacgatgga taaaacagtc attgtcaatc tgcattcagt
     3721 ccctctggct cgtgagtttt ctacgcgtgt gctcgcgtta aaagctgctc aggttgtctt
     3781 tgatggtcgt ccagacgact taaccgatga acgcttagag caaatttatg gcaatgcaat
     3841 ctttgaagaa aaggcaggcg aaatcgatga gcttgaaaga aaccgttcac tttaaatggt
     3901 ataaacacct gcttattctt ctgttcttaa ttgtttgttt tcaaggaagt gcctggatca
     3961 cgtcagctga tttttctcag gtttttcaaa actccaatca aatgactgtc tttttatcac
     4021 gcttcattca tccagacttt agctatttac ctaaattagt cgagcctatg gtgaaaacac
     4081 ttcaaatgtc tttgcttgga acggtcattg gcttaatttt agcgatccct gttagttttt
     4141 tagcgaccac tgtagtgaca gaaaaccgtc ttctttctac catctttcgt tttcttttag
     4201 gtttagtacg gaccatccct accctgcttt tagccgcgct ttttgttgct gtgtttggaa
     4261 ttggtgaagc gactggcgtg ttgactatcg ctgtctttac ttttggtatg gtgtcacaat
     4321 taatgtttca agcaattgaa accattgact ttggcccgat tgaagccgct gcggctgttg
     4381 gagcaaataa aatgcaaatc gctatttggt cgatcgctcc acaagttttg agtcagtttg
     4441 cgagctatgc tttttacgcg tttgaggtga atgttcgtgc ttctactgtc cttggatatg
     4501 tcggagctgg tggtattggc gttattctaa attcctcact tgcgcttttg aattatgaac
     4561 gagtttcaat cattattcta acgatcctag taactgtcgc gatcgttgat aaattaagtg
     4621 aacgtgtcag gaggtccctg ttatgatgct tcgtgaaatg aacagaagaa cccttttatc
     4681 attagttgct ttagtgattc tcgttatttt gtcggccgct actctggatt actctggatt
     4741 aagcaccttc tctccttcca tgggaatgga cgttttgaga gggttaggtc agcctgattg
     4801 gcagtttcta tatgatggca gcggcgagga tttgattagt ttgctattat taaccattgg
     4861 aatcgcctgt ttagggacaa tgatcgctac ggtattagcg attcccatta ccttgatcag
     4921 tgcggttaac ctctggcaag cacatccatg ggtaacaaaa attggcaagc ttatctgtaa
     4981 tgttttacga gcttttcctg aactagtttt tgcaattatt tttgtgaaag tcgtcggacc
     5041 tggccccttt gctggtgtta tggcgattgg cgtccatcag atcggaatgc ttggcaaact
     5101 ttttacagaa gaaatggaag cgatggatga gcgacttgtt gaagaaatgc atgcggtagg
     5161 agcaaatttt tggcaaacga ttttctttgt tcgcatcccc tatttgatgc cgatttattg
     5221 ttctttagca ttaaatcatt ttgaaatagc ggttcgtagt gcggctacct taggcttagt
     5281 tggcgctggc ggaatcggtg ctccattgat attcgcgatt caaactcggt cttggagcaa
     5341 agtgagtatt attttgattg gcgtagtcgt aaccgtgttt attttagatc aaataaccgg
     5401 aattataaga aagagattga gataagaatg aatatattgc atatatcaga tatacacttt
     5461 cgcagagagt atgaggcttg tgaagaaggc tataagggaa tgctggctac gatgcagaat
     5521 ccccttatcc ctctggagca atgcattcat catctcctgc agcaaacacc cttggacctt
     5581 gtgattatca gcggagattt aaccgaagat ggcgaaatag acgattatcg atacttgaag
     5641 acttggctgg aaaatgcgct aggagaaacg acgattcttg ttactctagg caatcacgat
     5701 atcaaagaac atttttgggt aggctggtgc gatgaagccg cctcttctgc cccgtacaac
     5761 cagatagtca aatatcctga gtttacagtg atttcctttg ataattcaag ctatggatac
     5821 gcagatggta tagtggacga tcagcaattt caatggctga aaaaagcctt agagcaagaa
     5881 aaagaccaac cgattttctt ggtcacccat catcatctac tgtctcacca aagcagtata
     5941 ccaaaatggc ctggtaccga acgtttcttg aagctaatcg caccttacga tattcgctgt
     6001 atcctaaacg gtcataccca tcacacattt actgatgaaa ttaatgggat cccctatttt
     6061 actgtatcga gtatgtcctt tgttggtgaa gatgaaggcg atggcttcgt acgctttgaa
     6121 gaacgtcatg ggtataacct ttatcatctc gatcaaggaa ggattgttcg ccagacaaca
     6181 gaaaacttta ttccaggcaa cattttaaaa acacttcaaa tgaaggatta aggcattata
     6241 aatcaaaaaa agagaccgaa cagctgaata cgcttcggtt tttctatttt gagtcagcta
     6301 aaggagcacg cagaatgaac attacgtttt tagagacaag tgatatgcat ggctatgttt
     6361 caccaacaga ctattccggc agaaccgatc tggccttagg aacggctaaa gtatcgtcga
     6421 aactgaagga attacgggca aaagcagatg gaccggtaat tacgatcgaa aatggtgatt
     6481 tcatccaagg gtctcctttg agttattatc ttgctaaaga atatggttca gccaaagagc
     6541 ttacagactt gattaaccaa atgagctatg atgttcaagt gttgggaaat catgagttta
     6601 actatggtgt ggattatctc gaggaagcga ttaagaatta tcaagcgcct atattaggag
     6661 ccaatatggt gaacaaaaat gaccaaccat actttggaaa cgcctataag attatcgaaa
     6721 aggtgggtgt aaaaattgct atcttgggat taacgacaca atacattcct cactgggaaa
     6781 agccagaaac cttagcagga attacgttta agagtatcgt cgaaacagca aaagaatatg
     6841 ttcctctatt aaggaaacaa gcagacttgg taatcgttag ttaccacggc ggatttgaaa
     6901 aagacttgtt aaccggtgag gacacagaag cgctaactgg agagaatgaa ggctacgagc
     6961 tattgcagca ggttgaaggc attgacgccc ttttcaccgg tcatcagcac cggaaaattg
     7021 ctgaaacggt tcatggagtt cccgttgttc aaccaggcta tcgtggcgag ttcatcggtg
     7081 aaattcgtct taccgtcgag aaaaaagagg ggcaagtggt tgttacagat caccaggttg
     7141 ctttacactc agttgaaaaa tcagagcctg atccagaaat tcttgcagcg atcgtaccgg
     7201 tagaagaaaa actagagaaa tggttggatc aacccttagg taaagtgaac ggaaatatgc
     7261 gtattaatga tccgatgcag gctcgtcttg aggaacatcc ctatgtagag tttattaatc
     7321 gtgtacaatt atttgccagc ggctcagaga tttctggaac tgcgttattc aataatgaag
     7381 gaaaaggctt tggtgagacg atcacgatgc gggacgtcat caccaactat atttatccga
     7441 atactctggc agtgattcaa ataaatggtg cagatttgag agcggcgttg gagcagaccg
     7501 caaattattt agctgtagaa aataaccaaa tcgtattcaa tccagccttt attcagccta
     7561 agccccagta ctacaattat gacatgtatg aagggattca gtacactatt gatatgaaac
     7621 agcctgttgg acaacggatt accgaacttc gttttaaagg tcaccccgtt cttccgcaac
     7681 aaatgctgga agtggtgaca aatcaatatc gggcaatcgg cggaggaaat tatgcgatgt
     7741 ttggtgcgga gaaaattgtc cgagaaatcc aaatcgacat gaccgaatta attgccgact
     7801 atttgaatat tcacccacgc atcgaagcaa aagtgaatca taattttcaa gtgattttgt
     7861 agtatataaa taaaaaattc aaaaaggtgt gagcagtttt ttgcccacac ctttgttatg
     7921 ccaaattgtt aggctgtctg atttccatcc agccattctt tagccagcga tctacctcat
     7981 caaaacaaaa aattttcctc tgatggttcc ttaaataaga ctattttgat cctttttcca
     8041 tgaataagat tttgcatcag acaatacttc atttctgaaa aagttgcggc accaatatag
     8101 cctaataccc ctttccgatc ttgattaaat acaaaaaata aatccgctgc tgcgatattt
     8161 aaaaaaaagt cgtcaagatt tctggatact ctttgtctag gatcggacat gagcgaggat
     8221 aatcaattaa ttcaatgcct tttttactga aatctttttc catatctcga tattcttctt
     8281 ggaaaacaat acttccagaa ataacagctt tcacaaaatt ggcctccatt tttttatttt
     8341 tcagacactc caccataccg gatacctctc aaagaaaaga catagcaaaa atcaatagca
     8401 gctatctctc cacggttttt tactggtaat tttatttttc ttttggaaat tgtatcagga
     8461 aggtcgttcc ttcatttttt cggctggtca cttcaattga accgtggtgc aggcgcacta
     8521 gttccttcac gattgataat ccaagacctg actcgcctat ctttgtattt ttacttgacg
     8581 gatcggcttt ataatagcga tcccaaatgt ttttttgttc ctcttcaatc atgccgatgc
     8641 cattgtcctc gacagtaata aatacaggct ctttgtcaaa tgatgttggt aaagagaaat
     8701 cttagaataa tttggacaga acagaaagaa gaaactctct ttctgttctg tttatttggt
     8761 taaaaatgtt ttgccatgac gaatcatgat ccgtagtttg aaattgtaaa agctgcgaaa
     8821 gccgtacgca ttgcgtttta atactttgat gtggttgttt aaacattcca atggtccatt
     8881 tgaatagctg tagtgaagcg cattttggat ctgtggcaga aactttttaa acgttcgtaa
     8941 agtggttgta taggtttcag gaagaccgct gacttctctg tttaaacagt cctcaaataa
     9001 tgactgatct cttttcttta ccgcataaag aatgtcttgg tataactgat agccttggcg
     9061 caggttggga caagcagata acagtcgatc gacaatctca ctttcagtca aataatcttt
     9121 aaaactggca cgccaaaccc ttttttcgta gtttaacttt gcttcttctt tttgtaggag
     9181 tttccaatag cgtttcaatt ttttccctaa ccctcgttga gagtgggaag ttcctttaag
     9241 ccgtgccgtg gtttctttca ctcgatgatt caagaaggtt ctgccaatat gttggacgat
     9301 gtggaaacgg tcgatgatca actgagcatt cgggaaaagc tttttcacca atgaaacata
     9361 aggcgcatac atatcaataa caatccattg aacggcttca cgagtcgcta atgaaaaccg
     9421 agaaaaatag cattctaaat aaggcaagcg tcgattttcg acaatatcca ataatgcatg
     9481 gctttgtccg tccatcatga taaaactcat tgcactggca acacttcgaa ccgatttgaa
     9541 ttcatcaaag cataagacct ttggcaaagc ctgtttgaaa ggattcaatg ggcggtagaa
     9601 tcgcttcatg acacgataga ccgaagaagt ggaaatattt ttctgacgag cgatttccgt
     9661 catggaagta ttctctttta agcgagcaat gacagaccaa cgaacttttt gactaatgaa
     9721 acagtgtttt tcagcaacag aggtatccgc tacgaatgtt ctttggcaag acttacattt
     9781 aaatcgttgt ttcttcaact ccagataagt gcgatactca gacgcatcat tcagtaacaa
     9841 ccgaacggtt gtcgtccccc aacgaatgat cctagagggg acgacacaaa gacaatggga
     9901 acactcttct ggcttgtaca gaagagttcc tttgtaaatc atggatttga gtcaatatct
     9961 tggacacatt ttagtagaga cggttaagcc attttttctt ttgctaattc ttcaaattcc
    10021 tgtggggtta gatagtgaat cgagctatgg attcgatttc ggttataaaa tccttcaatg
    10081 tagctaaata gcgctcgatt tgcttcttca aaatctgagt agctagtcgt gtagacttct
    10141 tcttttttca atgaggcatg gaaagattcg attccagcat tatcataagg tgttcctttt
    10201 ctactataag aatgccttat tttgttggtt ttaagccatt gttccacttc tcttgctgtg
    10261 tattggctgc ccaagtcagt gtgtagaatc attccttctg gaatgtgata atgtattttt
    10321 gctttattca atgtttgaat gacacaatcc acagtcattc gttttgaaaa tgtataacta
    10381 ataatttttt tcgtgtgtaa atccatgatt gaagataagt aacaccaacc atttttctta
    10441 gtaggaatgt acgtaatgtc agctgcccat ttctcacaga tcgtttcagt agaaaagtcc
    10501 tgatttaaga gattctcttt tgaaatgatc ggtttattag acctttgagg tctatatttt
    10561 ttaactacaa tcgaccttaa atttaattgt ttcattagct tttggacatg tttaagagat
    10621 actgaaatcc cttcttttaa taaaacttga tggatctttg tggcaccata gcgttgttta
    10681 ttcgtaaaaa aaattgttga aatcttcttt ttcaacttat tatttctgat ttctgtagct
    10741 gtcaacggtt tattcttata aaaataatag acacttctag ggatttctaa taaccgacat
    10801 aacaaagaaa cagtgtaatc cttacaccat ttttcaataa attgaacaag cgaatttagg
    10861 ttttccgaga gaatatggct gcggcttttt ttaagatttc aagttcctca tttaaacgag
    10921 cattttcttt tctcagttca gctacttctt ttccagtcag tcctgtggac tgatcaggta
    10981 aatataaatt cttccatttg taaatcgttg cttcagataa gccatattct ttcgctaacc
    11041 ctcgaacaga acgtccagtt tgattcagtt caacaatcat ttgtttaaaa ttttcttcat
    11101 atcgggtcat aaaaattcat cctctcgtag ataagtttaa tttattcttg tctctacgaa
    11161 aagctgtcta agatttgata ctaacaccaa gttatcttta gaaccatttc agatacatat
    11221 gaaacaatta catatgattt tttcataaaa aaacagtact gtctagtagt ataaaatata
    11281 caaatttctt aaaaattttt tcactaaaat cactacctga aacagtctaa aacacttata
    11341 tatcaacgtt taatgaaact atctccgttt catttcagat acaaataaat atagtcgggc
    11401 tccaaatccg aaaaaaaata cccccagtag cttatcctaa gctactggag atgtataagt
    11461 acggtcatat aacaacgggg tagggtaaat aaatgaaaaa gatggaagaa tcattattca
    11521 ataacttttt taaatacttc gttgtaatca gttacctgtc cgttcttaaa taccacagaa
    11581 taatcttttt tcccattttc aagtaaccga agtggaatgt ctttaccaac ttgctctgtc
    11641 actgcattac tgatacctgt tagattagct aataaatctg ataaagattg agcatctgta
    11701 gaaatctctg tatcagtatt tatcgtgatt cctttatctt catttacaag cttccaagta
    11761 ggtggtatag ctgtgtatgt ttcgatgaat gaatcaattc tttcatctat cgtcttccca
    11821 gtatctactt ctgtctcatt actcttagat gaatcgattc tttcatccat cgtctgctcc
    11881 gtaactgctt ctgtctcatt attctcaccg gaaactctct tcacacccaa acttatatat
    11941 tgtccatctt tagtgtgcat agttaaaatt cccacgcttt tactaataaa ttcactataa
    12001 ccagttcttt caatagagac ttcaatgtat ttcacactat catactcaaa agaaaaatag
    12061 acatgtaaaa gcattttgtt gtttacatct acataataat cctttgactc tacttgagac
    12121 cttcctatac ggaatacagc tttactgtct gatatctcta taccagcatc cttattatta
    12181 atctgccaat ctccaagtag ctcttctggc aagctagtag tttcttgtga acactttacc
    12241 acttcactgg tagaggtcga aacattcacc tccgcactgt tagaagaatt ttccaaggaa
    12301 tcaggagcct tagcacaacc aacaagtctt tatatattgt gcagccgata ttttcttcat
    12361 tcagtacacc cttgaaatat gcagtgttag ataaagcagt tcgtcctgat ttgctgaggc
    12421 acccatctct tcttgaacgt actccttgat tttcaaggcc gcccgatact ctttcggaaa
    12481 acgctgcttc actgaagcca gcaaaaaatc atccttctcc ggcaactctt ccttcttaat
    12541 cattcgggca ataaaaaact gtaaatgcgt cacaaatctt aagtaaagca tcgaatcttt
    12601 atcaaccttt ttttcgggaa acagatatcc tagcatagcc aagatatcct ttagtgctct
    12661 taccgagagc atggtttctt ccatatttgc ttgttcactt tgggcattta caatgtgaaa
    12721 cgcaatgttt ccagcctcct cttctggcaa ctgaacattc atttttttgt tgatcaattg
    12781 gatagcatag cagccgatct caaattcttt tgggtaatac ttttgaattt caatcagcag
    12841 gcgattttga aaaacaatgc ctttttgact gcgttcaatg gcatgcttca agtgatcaac
    12901 caaggtaaaa aatagacttt tattcagctt aaagtcaaac ttggcgttgg cataatcgat
    12961 aattcgcttg acgcaaataa ttagatcatc cggagcagcc tcaaacagtt taatgtaatc
    13021 cttttttaga gcatcagaaa gtgaatctaa tttaaatact ttttggattt tgtcagccgg
    13081 aaccaaatcg cctttcttta gttgaaatcc aatccctttc cccatcaaaa ttacttcttt
    13141 gccttgctta tctaagcaaa gaattaaact gttgtttaac gttttaatta ctctcattag
    13201 attcaccgtt ctctctaagc taagccattt cttgcgatta ctttttgata ccaaaagaag
    13261 ctttcttttt ttattcgttt taattcttta agattctcat cggttcggtc aacatatata
    13321 aaaccataac gcttattgaa gccttgatgg gtactaacaa catcaatcgc tgaccaaggt
    13381 aaataaccaa aaacatctac accatctgtt aaagctgctt gaatagccag aatatgtttg
    13441 gctagataat caattcgatc ctggtcatta attcgtctat cttcctcaac tatgtcgggt
    13501 gccccatagc cattttcagt aattaaaata ggtaaatcat agcgatcgta caatttcctc
    13561 aatgttaatc gtagtccagt agggtcaact acccagccat atggcgtttt atctacataa
    13621 ggattttcag ctgcccggta gacaccttct tctcccaaca tgatctgttg atcccccgct
    13681 ctagcggaaa catctgatcc gtcatttttg ctggcagcga tggtggcagt tgaataataa
    13741 ttaatcgcaa taaaatcggg tttaccggag gataaaattg cctgatcctc tgcctcaatt
    13801 ttaggatata atccacgatc attcaaatag ttttcaaaca aataattgta ttttccccga
    13861 actgctacat ctaagaaaga ccagcctctc aatgtttccc aattatgggc tgcaattgca
    13921 tcttctggac tggaggtagc ttgatacata gccgttaaat tgattgctgg accgattttt
    13981 cctttaagat tcaactgatg atacaagtga attactctag cttgagctaa taaaacatgg
    14041 tgattaattt gaaagagttc tttttttgaa ggcagctctc cacttggtgg caaaccaatc
    14101 gctccaggat gaaggatcat cgtgttttgc tcattaattg tcagccaata gttcaccttg
    14161 tcaccatact cagtaagcaa tacctgtaca tagtccacaa aataatcgat agttgctcga
    14221 tttaaccaac cgccctgctc ctctagaaaa taaggtaaat cgaaatggta catggtgaca
    14281 atcggttcga ttccctgatt caggcattca tcaattactt gatgataaaa agaaattccc
    14341 ttttgattgg ttttattatt ttcaatatcc ataattcgcg tccaagcgat ggaaaagcga
    14401 aaagctttta agcctaattc tttaaacagc tgtatatctt ctttatagtg atggtaaaaa
    14461 tccgaggcaa cttcaaaatt agctacccct tctgtatgct gatacttgtc aataatagac
    14521 ggttgtttcc catcctcctg ataggcaccc tctgcctggt aggcactaat ggaggcaccc
    14581 cataaaaact tctctggaaa tgctgaaagc tgtttgtatt gcacaaaaat cctctcctta
    14641 actaatttgc atcaatggtt cgtgttcctt taagcttccg cttgcttcac taattcctgc
    14701 gtaattttca ctgttggtca ctaccacaat cactgtatca tcgtagccag cgtttctaat
    14761 ggcagcgaaa tcggcgttga ttaataattg tcctgctgac acctgatctc ctgcttttac
    14821 aaatacttca aagggttccc cttttaaatt cacggtgtcg atccccacat ggatcaataa
    14881 ttcaatacct tctttagaac gtagaccaat cgcatgttta gattctaaaa tagtaatgac
    14941 ttctccctca acaggtgaat aaatgctgtt ttcttctgga tgaatggcta cgccctttcc
    15001 caatagtccc ttagaaaaga cagcatcgtt cacttcagaa ataggacaca cggttccttt
    15061 taccggattt tcaacaatga ctgcttcggc tattgccgtt tgctctgatt catcagaagt
    15121 atcgtcaaat ccaattagat aagcaatgat aaaggttgca acaaaggcga ttacacacgt
    15181 aataattgcg tgaacaatat tcattggatt ttctccaata aaggctggta aggctgctaa
    15241 gccaggggaa acaaaggcat atctgactaa accgctcaag ccagcataca aaccagcaat
    15301 tgcaccacca atcatggaag caactaatgg ttttttcagc ttcaaggtta ctccatagag
    15361 tgaaggctca gtaattccca ttaaagcagt gatcccagtg gacacagcta gctgcttcaa
    15421 ctctttgttt ttagttttcg cggctacagc aaaggtagca cctgattgag caatattaga
    15481 agccagcatt cctggcccat tgatcatttc agaacccgta ctgctcaatt gcatagtagc
    15541 gattggcgtc attgcccaag ctgtacccgt aaccaccaaa aatggctgca aaccacccat
    15601 caaaaatgga attaaccaag ctgctttgcc gttaatccat tcagccccca ttgctataag
    15661 atcgttcagc aacgttccaa acggtccaat gacaaccaac gctaaaggcc ccataattaa
    15721 aaataccagc atcggcttag taaagaattt aataaacgaa ggcgaaattc gatccgcaaa
    15781 gggctctaca taggacatga tccaaacagt aaagataatc ggtaaaactg atcccgcata
    15841 atctgcagct aataccggaa ttcctagaaa agaaacggca tcgccagtag aaaaaagctg
    15901 tgaaatattt ggatgaagca aaatgccagc aacagtaatc gccaaaattg ggttgacatc
    15961 aaactttatg gctgccccat aggctaatag tactggcata aaataaaagg ctgcatcagc
    16021 aatagtattc aaaatgatgt aagtttgact actgtctgat agtacaccag tcaaagtcaa
    16081 gacggccaaa atggctttta ccatcccggc acctgttata gctggaatca tcggtgtaaa
    16141 ggtggtagaa atgacactaa tcaatcttga aataatactt tcttgattgt ctgaagaggg
    16201 gcccttttta attgaagttc ccacttcctt ttgaatttcc ttgaaggctg tttggacttc
    16261 attgccaatc accacttggt attgtccacc gctatccacc acactaatta caccaagtgt
    16321 tttttctaag gctttcgtat cggctttcct tttatctttg agttcaaaac gcagtctggt
    16381 ggcacaatgg gttaagctga ccacattttc ttttccccca acctgttgca atacatttct
    16441 tcctacttct ctgtaatcca ttattctctc tccttaaagc agtaaaaaag accaagtcaa
    16501 accacgcccc aatcaacatt gaagtgttag tttgtcttgg tcttgcctgc ataatcagta
    16561 acatgccaat aacgttattt tgctgtaatc caataatagc acattatgat agcgcaatca
    16621 acagggaatt taatttttta tcttttcgtt tgaccgtata gggtaatata acagttagat
    16681 cacatcaact agggtatatc tgcgtatact tactgctaaa ataagtatat ctaatattaa
    16741 ctataccagt aaaaccattc ctacataacc agcgggaaat tcccaaaatc gtgaaagcat
    16801 caaaaatgca tataacggat cacacaactt caacaattct gctcttggga aaaattgatg
    16861 aatttggtga gtaaaggatg gaataaacag cattccaaca atataggcaa caatagtgat
    16921 acttgttttt ttggtaaaaa tcagtaagca atagccaatt tcaatcattc cgagatataa
    16981 aggtatcgat agaatgattt gggctagtat tcttaaaaat taactagcaa atgtttaaac
    17041 tgatttattt ctaatctaca tgtagtaagt atttcaattc caccaattat caataaaaat
    17101 accaaacata tccataataa ttttttcttt ttcatttctt ttatattgtt ataacagtga
    17161 ataacataaa aattcccgac aatcattgta atattttttt gtcacctgta ttagtattta
    17221 atttatagga aactataaaa actccctagt gaatttacac ctttgggagt tcctgtttgg
    17281 acggtatgca taatacattt aaatcaattt aacattaaca cctcagcata tggtatctcc
    17341 acatatatac ggtctttctt tggatagtac tcaatattta tgttgatagc acttccattg
    17401 tctaatatag ctttagcccg gtatgtgtta tctccatttt catgagtaat cgtcacatca
    17461 tcacatgatc gatttaagtt gtactgttct tgtaaaatct gtgttaccaa tggtttcgat
    17521 gacttctcta atccagatgt acctgtcatt tttgaaaaaa tgatacctat aatagccaag
    17581 acaacgataa ctataatgat aaacttcttg ttcatattta ctttattcaa ctttttatct
    17641 cctatctcca aaataatttg ctcttatccc tcttatcagt acattgacat ccctaaaagt
    17701 acaccaaaga ttgctccagc tacccctgct atcatcaaaa tcgtacccat ttgttcatct
    17761 ttatctcgat ataagtagcc cataataaca gcaacagcac caaatacaac aggaataaat
    17821 gctaacgcta ctaccataga aatccaacca ataatcaaat acagcttccc tgtctcttgt
    17881 ccatgtacga tttgttcctt catttcatta attgtctgct cagtactttt ctttactgcg
    17941 tctgctccag ttgtctcatc tgtttcttgt agtgctccgc aaaactggca atacttactt
    18001 tcatctgtta tttctttgcc acattttcta caatacatgt cttctcctcc taaaaacatt
    18061 caattgttta ttagttaact gtttacttta ttaaatacat cagaaaacag ctcattcatt
    18121 ggtacactta atatctcaga aagcatcttc atttgatagg gtttaggaat cgttctccct
    18181 gtttgccacg aacttacaca ttgctgtgta acattcattt ttttgctaac tccgtttgag
    18241 aaagtccttt ttcttttctg tatcggctga gattacttac ttttttcatg attctcccct
    18301 cctgttttat tatatacaac aatatgatgt atttcaactc ataaaatata aaaaaacttc
    18361 aaataccatt acaaatacaa caacttgttg tactatactg agatgggggt gtattaatga
    18421 aacagaaaca atatgcaaag gtcattagag aaaagcgaaa agctctcggg tggacacaaa
    18481 aagaattagc tgaaaaaatc ttctctacac aacaggcagt agcaagatgg gaaaattctg
    18541 taacagagcc taacctcgat agcttaaccg ctctttctag agccctagga acccctgtaa
    18601 gccattttct agacaatgtt gtcgtagatt atgaggaaga gtttttggct ctctatcgtt
    18661 ctcttagcac tgaagatgca gttcgaacaa ttgactatat gaagctatta aaaaggcaag
    18721 aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa taacccaccg aactttaacg
    18781 gtgggtgtat tgagatgaaa taagattaag agctatggat aacctgtcta gtttaataca
    18841 attgggtctt ttctcttcct tactctccgt ccatcatctc ttgttacact tgtaaattga
    18901 taaagaggaa ctaattcaac tcctgataat atcaattctt ttattacttc atctatccct
    18961 gctccttttg ctaggtagac taaaaaacta ctacttgctt tcacattgta tagtctctta
    19021 atatccttag aagctcgttc aagagtgaat ccttgtttaa caaagtagcc tctgttttct
    19081 tttaaagcat tttccacgct gttattccca acattacgta gacgttcaca aacagtgtta
    19141 gtaagttctt tctctgtcat tagatatgtt ccttgccacg ataagccctg taaaacttca
    19201 tgtgcttcat ctaaaatcaa ttgtttttcc ttgctaagtt ctgatcggga attttgagga
    19261 aatactcttt tagcctcttt ttcgttaaaa catatcttaa cccattcata agaaaaatat
    19321 tgatatgtaa aatgaaattt ttccagaagt ggaaggatat cttcattcaa tttttgaatc
    19381 acagaagttg cattaaaaac gtctaatcga tagacattgc ttctcttatg attaaagttt
    19441 cctaacgctt tctcttcctt taacctattt aggtattcga gcatcttcaa aggaacatct
    19501 tcatcatcta atttttttat caatcctata aaagtcataa gtgatagtaa cttattacat
    19561 ttatctatgt cataagtttt accatagtaa aaactcattt ttttagctaa ttgttccccc
    19621 gacaaaatac aagtcacact ttccttacta tcatcaaaaa aattaacatc agaaagcaag
    19681 acatcataga cacgtcgcat ttcctctaga taattcctta ttgtcctgaa aagtttgtct
    19741 ttcattgatt caagagtgtc aaaaagtact tttgattcct ctttcaattt tcgaaaatgt
    19801 gtaggataca agtctaggtt ctctaatagc caattaaatg tttctacccg actgtctttc
    19861 tttaaaatta gcatcacaac tccaataaaa ttttcactga tatcacaatt actggaatga
    19921 caataaaaaa tggaatgccc agttttactg gtaaaaatac tagcagaggg attatgatcg
    19981 ttgtgaaaca gacatcttaa gtttttactt gacttaagta atttattcat atcaatggtt
    20041 aacaatcttt cataaatctc attaaaagac atgtcggagg aatcaaacag gcagtcttta
    20101 aaccactgtt tcatttcttc tttgtcgtca ttttttatca acttaacaaa atcggtttca
    20161 ttgactagtt catactctct ccctgaatca ctttgacaat ttttagaaca tgtcatggta
    20221 aaatcaatcg gaagctcagt aatatctaac atgttgtctt cattaattat tatcactgca
    20281 tctgacttat atcctccaaa aaacattcta ttcgaagcat ttacattgtc atcacacccg
    20341 gggatgttct ttttcaagta ttcgtaaaca gcaaccattt taccatagtc agtaattgct
    20401 tcttttaata gaaagacaat cttgtatttt tctagatttt cagagtaccg aatacttttt
    20461 tgtatcgcaa acgcagaatt atgaactaaa ttgatagata aaaccttttc tatgcttaag
    20521 tatctactat catctttact caaatctact accttcccct tctcatcttt aacagtgtta
    20581 tcgacatcca gaataattac tctggtttcc acaaaactct ccttgtcatt gcctttattc
    20641 tttgtcttgc ttggaataaa tgattgtgat gaatttagta tctctacaaa ctcatctctt
    20701 gtaacttctc tatagttatc taaacaccta tctcttacat cagttttttc tttttttgta
    20761 actggcttct ctaataattt ttctttatct acataaattg gaatattaat cattgatgtt
    20821 tccatttgtg tttcctcctt ataaaattct gttaaagact aaaacataca gataataaaa
    20881 acaaactctt tattactata tgttttagtc ttatttgttt actcaacctt tataccgttc
    20941 catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt ttataacagg
    21001 taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta gatattcact
    21061 tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt ctagcatagg
    21121 ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa caatgctatc
    21181 aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa attttctaag
    21241 aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg attaatcaaa
    21301 caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa agaggtgtca
    21361 atatctttgt aaaatttatt tacaacaccc ctttttcgag gtatactaat tttaggaggt
    21421 gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa gatactcgga
    21481 acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt tggtcttatg
    21541 attaacaacg gtcaattaat tgacaaaaaa actatctacg aatgggagaa gggaacctat
    21601 ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag tatagaggag
    21661 ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag agatagcatt
    21721 gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg tcaacagttc
    21781 ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt agaaccagag
    21841 atgcaagaat ttattgctaa caagacttgt aataaagtta aaaatgaaaa aattagtctt
    21901 ttcaatatct taaaaataga agaattattc atcaatgcta ttgtagaaga atttgacaat
    21961 aacattcttt tcctcactag cagtatagaa gaactactag aaagaatggt agatgaatgg
    22021 ctacctattc aattgaaaga catgtcttat cccgaagaag ctgtgagaga aattactgat
    22081 aatataaaca agctagaaca aacgatttca tcaattggga aaaaatacac taagaaaaaa
    22141 atgaagggag gtgataccat ctagtggtaa gtactccttg taaactaaga ggagatgatt
    22201 tcatggcaaa aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg atgtataaag
    22261 gacacattgc caccgatcca cgaactggtg aaaaaataaa tactacgaga agaggtttta
    22321 gtagcaaaca agaagctcaa aaagattatg acgagtacag acatcaaata ttatacggtg
    22381 ttaaaaagaa agcacctgat atgacatttg aagacctgta taatgaatgg ataactcatc
    22441 agcgtaccag tgttaaagct tcaacaatcg caatctcagt aagatacgca aataatcaaa
    22501 tacttccagc atttggtaag cttaaactat cgaatatctc tgtcccatat tgtcaaaaag
    22561 tggtagatga atggcacagt aaatatgaaa gttacgatta tatgagaaaa cagactgcac
    22621 aaattttacg ctacggggtt gcaatgcaat atattgacaa taatcctatg gaaaaaacac
    22681 ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa gaggagctta
    22741 ataatttact ggatgctttt aaagattttg gtaacatgaa acaatacgcc ttcttcagat
    22801 tattggcata cacaggtatg agaaagagtg aagtactcgc tttacagtgg aaagacatag
    22861 acacgtttaa taaagaacta catgtcaata aaactcttgc tgtagatgaa tttggtaaag
    22921 ttataattca atctccgaaa acaagagctt ctagacgagt tatctcttta gatactgaga
    22981 ctctttctat cctcaacaac tggaaattac agcaaaaaga agagtattta aaacttggat
    23041 acaatactag ctctaaagag caacatgttt ttacaacagt taaaaacact ctatacattc
    23101 caaacactgt taatgattgg ttaagatata ttttgaaaaa atataacttg cctcgtatta
    23161 cacctcatgg gtttagacat acacatgcta gtttattatt agaagcagga gaatctgtca
    23221 aggtagttca acaacgatta gggcatgaaa atagtaaagt gacattagac atctatgcac
    23281 atattacaaa taatgcacct aagaaaacag gtcaagactt tgctgatatg atggctcatc
    23341 agtaaaagag ctggtcaaaa tctggtcatt tctagtatac aggcaaaaca aaaaagctag
    23401 aacccttata tatcaagggt tctagctcct ataacaggtt tacccaacac tgccttccat
    23461 ctcataactg atcaatcgat tgagttcgac tgcatattcc attggaagtt ctttggtgaa
    23521 tggttcaaca aagcccatga cgatcatttc agttgcttca gattcggtca ggcctcggct
    23581 catcagatag tagagttgtt cttctgagat ttttgagact ttggcttcgt gttctaaaga
    23641 gacttggctg ttgtggatct cattgaatgg gatcgtatcg gatttcgacc attcgtccat
    23701 gatgatcgtg tcacattcga tatgagagat cgaacccgca ctgtttttgg caaaggttac
    23761 ttgtcctcga tagtttactt ctccgccgtc ttttgaaatt gatttagaga cgatagagct
    23821 agatgtattt ggtgcattgt ggatcatttt tgcacccgta tcttggattt ggtttgctcc
    23881 tgcaaaggcg atggagagca tggtgcctcg tgcacctttt ccatctaagt aaacacttgg
    23941 gtatttcatc gttgtttttg cacctaggtt tccgtcgatc cattcaacgg ttgcgccttc
    24001 gtatgctttt gctcgtttgg ttactaagtt gtacacattg tctgaccagt tttggatcgt
    24061 tgtgtaacgg caatacgcat cttttcttgt aaagatttct acgatggcgg catgcaggct
    24121 gttacttgaa taagtaggtg ccgtacaacc ttcgacatag tggatgcttg ctccttcgtc
    24181 aacaatgatc agcgtacgtt cgaattgccc cgtgttttcc gcattgatcc ggaagtacgt
    24241 ttgtaatgga acatcgactt tgacgccttt tggcacgtaa ataaaggtcc cacctgacca
    24301 tacggctgaa ttcagcgctg ctaatttgtt gtcggttggc ggtacaagtt ttgaaaagta
    24361 ctctttgaat aattcagggt attctttcaa agcagagtca gtgtcagtaa agacgattcc
    24421 taacttttcg aattcatctt tcatattatg gtacacgact tctgattcat attgtgcgga
    24481 agcaccggct agataagctc gttcagcttc tggtatacca atcttttcaa atgtatcttt
    24541 gattttttct ggtacgtctt cccagtcacg agcgggtttg tcacttggtt tttggtaata
    24601 tttgatggca tcgaaatcga tgtctgaaag gtcggggccc catgtttgca ttggcatttt
    24661 gttaaatgtt tctagagatt tcagacggaa ttcaagcatc cattctggtt cgcctttgat
    24721 tcttgacatc tcacggatca cttcttctgt caagccttct cctgtactga aaaccggttc
    24781 gacatcatcg tgaaacccga atttatattc ttctaattct ggtacgccca tgttctcact
    24841 cctcttcctt gtggtaatga tggaaagctg cggtaccatc gttttccatt gcttttccta
    24901 gtgctttcca ggcaagtgtc gcacatttga tccgagcggg aaatttagct acaccaccaa
    24961 gcatagcagc atcgcctaat tggtcgggat ctttgacttc ttttccttgg acaagctgta
    25021 gaaagtctac agttaaatgt tctgcttctt ctattgattt tccgatgacg gcgtctgtca
    25081 tcatcgaggc acttgccgta ctgatggagc agccactgcc gtcaaaggcg atatttttga
    25141 tgattccgtc ttttacatcg acttgcaatt cgatcacatc tccacatgtg gggttgttca
    25201 attcgatctt gcttgtcgag tcagtcaagc ttccacgatg atgcggatgg gaagaatgat
    25261 ccaagatcac ttgacggtat aaattgtcta atttagatag tcccatgttg gaaaaactcc
    25321 tttgttgcca tgattgcttc aactaaacgg tcggcgtctt gcgttgtgtt gtagaaataa
    25381 aagctcgcgc gagcagtcgc tgatacttca aggtacttca atagcggttg cgcacaatga
    25441 tgacctgctc tgacggctac cccttccatg tctaaagctg tcgcgacatc atggggatgt
    25501 aacccatcca aattgaatgc aacgactcct gtccgaaaat tcgggtcttg tggaccataa
    25561 acagtcaatc catcaattgc ttgtaatttt ggcaacacat aagcggctag ttcttcttca
    25621 tatcgatgga tattgtccat accgagtttt tgcaaataat cgattgcatg gcctaatgcg
    25681 atagcgccgg cgatatttgg tgtccctgct tcgaatttcc aaggtagttc tttccatgta
    25741 ctgtcgtata attcgacaaa atcgatcatt tcgccaccaa attcaacagg ttccatttct
    25801 tctaaccatt ttcgtttacc atataaaaca ccaatacctg ttggtccgca cattttgtgt
    25861 ccgctgaaag caaagaaatc acagtctagt gcttggacat caatggccat gtgcggggca
    25921 gattgagcac catctactac gatgaccccg ccgttttcat ggacaagttc agctaattct
    25981 tgaacaggat tgaagacacc taagacattg gatacatgag caatggagac gattttggtt
    26041 ttgtccgtaa tctttttcgc tgcatcctcc atatccagga agccttcttc tgttagttcg
    26101 atatatttca agacagcacc tgttcgtttt gccaactgct gccacgggat aacattagaa
    26161 tgatgttcca tgtaagagag cactatctcg tctcctgctt tgatatagcg gtcgccgaag
    26221 gttctggcta cccaattcaa gcttgtcgtc gtaccacgag taaacagcac ttctgctgtt
    26281 tcttttgcat ggatgaacgc ccgtactttt tctcttgctt gttcataatc atgggtcgcg
    26341 cgttctgcta acgtatggac accgcgatga acattggcgt tatcgtgttc gtaatagtac
    26401 gctaatgcct ggagtacttg ttttggcttt tgggtcgtcg ctgcattatc cagataaact
    26461 aaaggttcat cattgacgtc ttgaaataag ataggaaaat cattgcgtag ctttttagca
    26521 tcgatcatcc attcaacttc ccttcgatta cttcgactaa ttctttccga acttgttcta
    26581 cagggattgc tgttaagaca gaaccgagga atcctcgaat cactaagcgc tctgcttctt
    26641 ctttatgcaa tcctctactc atcagatagt acatttcttc tggatcaact cgtcctacac
    26701 tggcagcatg tccagcagtt acttcgtttt catcgatcaa aagaatagga ttcgcatcac
    26761 cacgtgcttt atcagaaagc atcaagacac gactttcttg ttgggcatct gcaccttttg
    26821 cccctttcaa gatgtggccg ataccattaa aggtcaatgt tcctttttct cggatcactc
    26881 cgtgttgcaa aatatgcccg atcgtatgag gggctttatt ggttactcgt gtatcgattc
    26941 cttggatttg tctgcccgag ctgattgcca cgattttgac ttctgcgtgt gcaccttctc
    27001 ctgctaaatc tgagtcaaag tcagcgatca catgaccgtc attcatgaca ccaattgccc
    27061 agtcgacaga agcatctcgt aaaatgtgcc cacggcgatt catataagaa gtaatatttt
    27121 ctcctaattg atctacggct gaatatttga tttttgaacc agctttagcg atgacttcca
    27181 cgatgatatt acctgatgat tttgctactt gttctttggt tgtctggaaa cgttccagat
    27241 aagaaaattc actatgttca tctgccacga tcaacacatg cttgaagaag tgctcatcgg
    27301 atgcgccatc ttgaatgaat aaggactcga gtggttcatc gatcacgaca tttttaggca
    27361 catacaggaa aataccgctg ttcataaaag ctacatggag tgcagtcaat tggtcttcgt
    27421 tcatgtttac tgcttttttc atataatatt cttggactag ttctggatat tcgatcattg
    27481 cagtgaacaa gtcggtaaaa atgactcctt tttctgctaa ttccacaggc agttgttcaa
    27541 aaatcgtcca tgaaccttgt tggacaagca caggattgtc tttcattgca tcaaatgccg
    27601 gaatcgtgcc tgtttcactg atggtttgtg tttctttcac atcaaataac gaccagcgat
    27661 ggaatttcac ccgatcgata tgcggcaatg gcagttcatc tgctttcgct agtgcgtctt
    27721 gacggaaggt tctcatccaa tcaggttcgc ctttacttaa agaaaaatca tttaccgcgt
    27781 caagataatc gagccagttt ttttctttca ttcttgctcc cccttacgct tcttcttctt
    27841 tatagtcgat tcctaattct ttactgatac cagcgtagcc ttctgcttcc agacgtttag
    27901 ctaggtcagc attccctgtc atgactactc ggccttccat catgatgtga acgacatcag
    27961 gtgtgatata gttcaataat cgttgatagt gggtgatgat caatgcaccg aaattgtctc
    28021 cgcgcatttc atttactcct ttagcaacga ctttcaaagc atcgatatcc agacctgaat
    28081 cgatttcgtc taaaattgca aatgtaggtt ctaacatcaa caattgcagg atctcattcc
    28141 gtttcttttc tccgccagaa aaaccttcgt tcagataacg ttcagccatt tcttctggca
    28201 tatttagtaa agccattttt tcatctaact ttttcaaaaa tgccataaca gaaattttgt
    28261 cgttttcatc acgtttcgca ttgatcgctg cacgcatgaa ttccgcattc gtgatacctg
    28321 gtatctcact tggatattgc atagctaaaa ataagccaag tcgcgcacgt tcatctactt
    28381 ccaagtctaa aacatttttc ccgtcaaaca aaatttcgcc ttctgttact tcatagtttg
    28441 gattgcccat gatagcggca gataaggtag attttcctgt gccgttaggt cccatgatgg
    28501 catggatttc tccggttttc attgttaggt ttacgccttt aaggatttct ttttcctcga
    28561 tcgacacatg tagatttttt atttctaaga cagacatgca ttataccccc aaattctctt
    28621 tcgttctcac tcattttaga ctaattgaga atgaagcgca atgaccttcc ttactaaaat
    28681 caaatatctt ttatttatta ttaaacaatt ttgatttcta aattagaata ggtataaaaa
    28741 agatgagaaa taagccatta cagacttatt tctcatcttt tttgttccat accttatttt
    28801 gcatcagcct ttactggttt aacagagccg ccccatttgt ctgtgatcca ttcttggact
    28861 tctttgcggt gaagcacttc aatcagtttt ttgattttgt cactgtcttc atcaccttta
    28921 cgagtcgcaa taatattcac gtaaggagag ttgtcctttt caagcaatac ggcatcttct
    28981 aatggattca accctgcgcc gtaagcgaag tttgcgttga ttgcaactag gtcaccttca
    29041 tcattttggt acgtagaagc taaaagtgat gggtcgatcg tatggttgaa cttcaagttc
    29101 ttagggtttt cttcaatatc atcgaatgtt gctgtttcca gatctactcc atctttgact
    29161 ttgactaagc cagcatcttg tagaattgtg atgatgcgtc cccagtctga ttcagaattt
    29221 gaagtaatga ttgttgcacc atcttttaat tcggaaacat ctttgacttt tttagagtac
    29281 aagcccattg gttcgatgtg gacagcaccg gcattgacaa agtcatatcc tttttctttg
    29341 atttctttgt tcaaataagg aatatgctgg aagtaattgg catcaatgtc gccttcttcc
    29401 aaagattggt tcggtaatac atagtcatcg aatttgacga tttcaagatc gattccttca
    29461 tctttcaata atggtttcac atgttccaaa atctctgcgt gcggtgtcgg tgaagcgccg
    29521 actttcaatg tcgtgtcgtc tgctttattt gctgaatctt tgctgtcgct gttgttaccg
    29581 catgctgcta atcctacagt cagcaaaagc gctgctgcaa atccaaaaat tttctttttc
    29641 atatcctaca tccccctata tcctattatc ttttgtctgt tttcttagtt aagaaatcac
    29701 cgattccttg aacgataaat acgattagta aaataattaa agtcgcaacg aatgtcactg
    29761 ttagattacc acgctggtag ccttcctgcc aagcaagacc tccaagtcca cctgcgccga
    29821 tcgcgccggc catcgcagta aatccgatca tcgagatcgt tgtaactgtt aatccagaaa
    29881 tcaatgcggg aatactttca ggtatcaaga ccttccagat gatttcccag taacttgctc
    29941 ccattgcatc tgctgcttcc agtacaccag ggctgacttc acgaaaagca atctctacta
    30001 gacgggcata aaatggtgct gcggataaaa caagagctgg aatggctgct tgcgcaccta
    30061 gcatagtgcc tacgattgct ttagtaaatg gaatgatcaa aaccatcagg atcataaatg
    30121 gcgttgaccg gaaaatatta ctgatgatcg atacgattcc ataaatgatt cgagcatacg
    30181 gtttgttttt gcgtccaata gagtaaagga tcaatcccaa aactaatccg atgatcgtca
    30241 ctaagaccat tgagattaat gtcatatata gtgtatcaag gatagcctga ttcatcgttt
    30301 ccgggttgat ttgtttaaaa tctagatatg tttctgaaaa gcttttatcc acgatggatc
    30361 acctcaattc ctactccttg ttctttgatg aacacttctg cttgatctaa ttctttggtt
    30421 tcaccagtca tcaagacagt caatgaacca aaggaacctt ctttcacttg tttgatcttt
    30481 ccatacacaa cgttgacatc gatgttatag cgtcgaatcg cttgggaaat gactggctcg
    30541 ttggcatttc tttcgctaaa ggtcaaagtt gccaaacggc cgcttggatt ttctttgatg
    30601 agttcagcta atagttctgt cgaatcagct tctggttcga tgtcttgttg tacaaagcgt
    30661 ttggttactt cttttttcgg ttgtctgaag acagtcaata cgtctccttc ttctacgact
    30721 ttccccaact ccatcacagc gactttgttg cagattttgc ggatcacatt catttcatgt
    30781 gtgatcaaga cgatggtcaa attcaatttc ttattgatcg ctaacaataa gtctaatact
    30841 tcttctgttg tttgcgggtc caatgcgcta gtagcctcat cacaaagcag gatctcagga
    30901 tcatttgcca atgctcgtgc gataccgacg cgttgtttct gtccgccgga aagttgtgaa
    30961 ggatatgctt gtccgcgtcc ttctaacccg actaaacgca acagttcttc tgcccgttct
    31021 ttacgtttgc tctttggtac gccagctaat tccagcggca gctggatatt ttcaagtaca
    31081 gttcttgacc ataacaggtt gaaatgctgg aagatcatac cgattttctt tcggaatgta
    31141 cgtaattctt tgttttttaa ttttgtaata tcttgatcgt ttacgatcac ttcgccgctc
    31201 gtgggaagct caagtccatt caggagacgg actaatgtac tttttcctgc gccagaatag
    31261 ccgacgattc cataaatatc ccctttttca acgtttagtg aaacgtcatt gacggcttgg
    31321 atgtttccgt ttttcccatt gaatgtcttt ttgacattcg ttagttggat taatgacatg
    31381 ccattcacat ctttctctta taatttttca aacaaaaaag ccttcgccct cttgccttaa
    31441 gcaaaaggac gaaagcttga acttccgtgt taccaccttg atttaccgtt acttcgcaat
    31501 aacagcctct tccagtactt ggacttagct tctttccgta tactgtcgcg ctgtaacggg
    31561 cactcccgta gtaagtttgc ttacgctcgc ttactctgct ctaagaccat cttccatctc
    31621 attttcgtta cccattttca gcaaacaggg ctctcttcaa acgtccgtga catgtactct
    31681 tcttttcaaa gctttctcgt ttcatatagg attgcttatg attatagcaa ctggaagaat
    31741 cagtgtcaac ttttttcaag tgattttctg attttataat tcgtcaaatt cttttggatc
    31801 aacatctttg aaacttaaga tccacgcatt catttcatct gaatcattca agatattgat
    31861 atcttcaccg attttatcat taacagaaga aactgtaccc gttagcgggc tggcaaattc
    31921 attgaccgct ttttccgctt caacttcgat cagaggttca tcttttttta gcgattgtcc
    31981 tgcttttggc agattggcaa acgtgatgtc tccaagttct tcttgcgctt catttgtcaa
    32041 accaacacag tattcttttc cattaaataa gatccaaagg ttgtcctttt tcttcaaatg
    32101 cttgcctgcc attttatatc agttctcctt cataaatttt ttcattgaat ccaattgcaa
    32161 caagctggtc ctctcgtaag atcagtggtc gcttgatcag cataccatct gtcgataaga
    32221 cttggctcgc ttttgaagcc gtaaactcgc ctacttgatc tttcagtcca agttcccgat
    32281 atttcatccc gcttgtgttg aacaggcgac ggattggtac ttgactcgtt tctagccaag
    32341 tctcaatcgt tgaggccttc ggtacttctt tgatcatatc aacggtttga taactaacgc
    32401 catgctggtc taaccacgct ttggcttttt tgcatgttga acatttcggg taccagtaaa
    32461 aggtgtacaa ttttcttacc tccttgaaga tcttgcaggt gcttcatttt ggtatctcat
    32521 cgataatatc aacccgatac cgatcatgtt ccctaaaatc gaggatcctc cctgactaat
    32581 gaaaggcaat gggatccctg ttaaaggaag taatccgata tttgctccaa tattctcaaa
    32641 gacatggaac agcagcatca tgatcaaacc gcttgcgata taggcgtaaa attcattatt
    32701 cgtatcgaaa cacacccgaa tcatacgata gattaaaata aaatacaaga aaatgacgaa
    32761 tgcaccaccg ataaagccga agttttcacc gataacggta aaaatcatat cggactcacg
    32821 aacaggcaca tagatattgc ttacgttgaa tcctttacca aacaagccac cggtaccaat
    32881 tgccaaaagt ccttgggccg gctgataact cattcctgac gtatcatgga aagggtccag
    32941 ccatgaatca atacgtgcaa actgataaga tttgaatcca actttataaa gcaagtctct
    33001 tccgccttca gtcgtaacca agaagatcgt tcctgctcca acgactactg ccaagattac
    33061 tacaggtgcg atgatgcgcc aagagattcc agacatcagg aaaatcccgc caaagatcgc
    33121 aagaaagaca agcatcgttc caaagtcatc ttgggccatg atcaatgcta aaaccggtat
    33181 tgtcacgatc aagagcttcc caatcagcca aaagtccgat ttcatcgtcc gttcttttac
    33241 ttgcgtatta tgtttagtta cgatcagtgc catcatcaag atgtacgcaa tcttcatcaa
    33301 ctctgctggc tggaaagtaa acgcaccgaa tcgaaaccag tttttagacc ctgttgaagc
    33361 agctaaattc tgatcataga atttgagcaa gagtcccatt accactaacc ccaaaccata
    33421 aaggtaagga gtcagtttcc agatccattt ggaattcaaa tgcatgatga caacgattgc
    33481 tgccgctcca actaaatacc agacaacttg catccctacg cctttcaaga ctgctggatt
    33541 gttcggatca tggctaagtg ctacatataa tgagagcaga ccaatcaaac ataacagaaa
    33601 gactggtaaa atcacgccat aatcaatacg attatcgttc gaagctttta attttttttc
    33661 catgtttcat cccttttctt aaaaaccata ctgctgatta ttatagcggt ccagttagat
    33721 aatggaaagt ctttccatag acatttggga aaaattaact ttccgtttgt gcagttgctt
    33781 gagcaaatgc agtacaaaag cctactgctt atctttagtg tatcatagaa ctttttttga
    33841 tacatgcaat tatctaccag ctgagattct cctcgctgtg agaagtttct gtgctttcat
    33901 tgtatcgaag gatctcttcg gcaatctcta gcagctgatc agaaaccaga ttggttcgtt
    33961 ggatgaaata aaaatatcga tggaaattca acggagtcat tggcacattt tctggaacag
    34021 agcgcttaga caaaatcgag cgaccgattc cttccttcag caacgcaaca attacatcgt
    34081 tacttttcac gatcatcttc ttatcttgga tattcatctc ttcaaaataa cgctgcgtgt
    34141 aatgaaaaac accagagttt gcttctctta caagccacaa tttgcttttt gggtccccag
    34201 cgatgactag ctggtcttcc atcaaagagt atctttgtac gccggaagtc tctaatggct
    34261 tttcgataaa accaaaatcg gattcatgtt tcgctacttg ttctaatgat tcatgagaat
    34321 tcgtgatttc gatctcaaaa tgcactttag gaaacctttt gatcagatgt ttcatcaact
    34381 gaggcaaaag atagacagca aaggtgtggg aagcaacgat ccgacatact tcttcttcat
    34441 tttgttcatt gagcaatttt tgctgagttt cttcccattc ttctaacaaa accagtgtcc
    34501 gttcatataa taagtcggct tgcttagttg gaagcatctc ttttcttccg ttacgttgaa
    34561 aaagatcgat attcaattcc cgctccaact gtttgatttg attcgagact gctggttgag
    34621 ataaaaacag tacttctgct gctctggaaa aatttcttgt ttcatatact gctcgaaaag
    34681 tcgttaataa cttaaacatg gcaccacttc cataactttt tatgataatt actataaaaa
    34741 ctatttgatt taaatataca aaaaaccatc tataatagaa atgtacaaca aaacagttgc
    34801 tgatccaact agtaacttag atgaagacaa tcagctgtct gcttgctcgt aatcatacat
    34861 tcctattcat tttacaatgt attcgttcat cttggtacta aaatctatta aataaaaagg
    34921 agaatattat atatgaagaa ctttttgaaa attattccga tcccgatctg cgggttgatc
    34981 cttgggttag cttcccttgg aaatctgttt aaagactatc accatgttgc tttaggcaat
    35041 ctaactggcg gtatctctat gattcttatg atattcattt ttgttaaatt gtttatctta
    35101 ttcgaacaca cgaaacaaac gttgcatgat ccaatcatcg cctccgtttc accaactttt
    35161 acgatgtcac tcatggttat ttgtacttat tttgtttcat atgaaagtat tgcaccaatt
    35221 gtcaaattca tttggctgtg cgctgttatc ttccaagtga tccttgtctt ttatttcaat
    35281 tatcaccatg ttatcaaagc tgatctttca attgaagcaa tttatccaag ctggtttatc
    35341 atttacgttg gatttggggt catcactgta actgccggga acttcttccc acttattgga
    35401 aaaattttct tttggatttc attgatctgc tatgcgttct tattgccgat catcatcaaa
    35461 cgtgtctttt tcgtaagaaa tatggctcat ccgacacgtc cgctgattac gatcgttgca
    35521 gcacctggtt ctctttgcct aacaggttat cttaaaaact ttgctgaacc taatttttat
    35581 cttgtcgtag cattgttcgc tttatctcaa gtattgtatt tcgttgtgct gtctatgctt
    35641 ccacgtttgt tcaagcttcg tttttatcca agctatgcag cctttacatt cccgcttgtt
    35701 atctctgcaa cagctatatt tactactgtt cattacttca acagtttagg tatctatagt
    35761 agcgtattgg atttcttaaa agttgcggaa tgtctgtttg caagtgtagt tgtcttctac
    35821 gttcttgctc attacgtacg tttcttaatt aaagaacata gcaaacatgg aaaatttgtt
    35881 tctaaaaaaa tcgcttaata atcgattcaa taggttgtga aaaagctgtt ctgcatcaag
    35941 taataagaac ggccaaacaa aaacagctgt tcatgttttt gtactggttg ggcttattac
    36001 cgcagatgca agcttttgaa caccgtttac caagaggtcg taaaagaagt ggtcagcttc
    36061 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    36121 ttccgtagaa gctacttctg gaacaccgtt tattcgaaac aagaggtcgt acaagacttt
    36181 tgtcacgacc tcttgttctt ttgtcaaaat actggtttag tgctactatg ttcgagtatg
    36241 cttttagtat ttattgacga aaggaaccga tcaaatgaaa tcattgtcac atccaataaa
    36301 atctgtaaag gattcagcgg tccgcgaaat cttacccggt ttactgcttt cttttgccat
    36361 cgctgccttc agcaggcttc ttgctattta tctgccacag ttaggtggag cgactattgc
    36421 tattttattg ggtattttat taggaaatac tttttttaaa cagcctgttc tagcaatcgg
    36481 cacaaaattt gctgaaagca aactattaga attctctgtt tttttactag gtgcaactgt
    36541 cacagctcaa acgattgctc aaattggtgt aaaaggaatt gtttttgtgt tactgcaaat
    36601 gtcagtcact atcatcggtg cttacttgat tggtaaaaaa ctgcaattca gcgatacttc
    36661 ttctttgatg atggctggag gaaacgctgt ttgtggttct tctgctattg cttcaatcgc
    36721 cccttccatc catgcgaaag aggaagaaaa aggacagatc atcacactag tcaatctact
    36781 gggtactgtg atgatgttga ctctcccttt tcttggatta cagttgtttg gtggaaatct
    36841 cattgaaaaa agtgcacttt tgggtggtac actccaatct gttggtcaag tagtcgctgg
    36901 ggcaagctta cttgacaatt ctgttgtcga attcgccatg ctatttaaga ttctacgtat
    36961 tatgatgctc gttgttgttg tctatctgtt tgaaaaattc atgcacaaga aacaaccagc
    37021 tctgtttgaa acgccaactg atacgcagaa aaaacgaaaa ttaccgcttc cttggtatgt
    37081 attagggttt gtgatctttt gtttggtcaa cagcttgttt tctttacctg tagttattga
    37141 ccaatcggca cattttatca gcagctggtt tgagatcact gctttagcgg caattggtct
    37201 gcgtttagac tttgcaaaat tcatgaaaga aggtacacgc ttcttatctt atggattagc
    37261 tgtcgggctt ctccaaacag tgatggcttt actatttatt tggctgatca aagtttagaa
    37321 tgaacatgaa aaaacagttg tgattctcgc atggaacaat gtccgaatca caactgtttt
    37381 taattttgca gatatccttc taacaacaac aattggcgtt tgatttcaac cccatctatt
    37441 gcgtttccca tatatcctgt cagttttcca ttttgcccga tcactcgatg acaaggaata
    37501 acgattggca atggattggt tcgatttgcc tgcccaatcg ctcgcacagc tttcggacta
    37561 tcaacagcaa cggctacttc tttatacgtt cggatttctc cataaggaat ggtcgacaaa
    37621 gctttccaga cattttgctg aaaaggcgta cctctcttta tagataaagg aacatcaaat
    37681 tcttttcttt ttcccgcaaa atattcttca aactgttctt gtgtgcgcgt taaaattcgc
    37741 tgattcgctg attcgctttc tgaaattggc tgaaaagata cagcagtcaa tccggattca
    37801 tctgcatcta accataaaaa gccgataggt gtgtgtattt tcattttgtt tgctcctagt
    37861 caatagactg gaacttcctc ctttttctct atccaaaact acttgccaac taataaaaat
    37921 tgttcttaaa agtttgataa accgaggaaa catggtaaac taaatacgtt agtttacaaa
    37981 aagaatacct gttcttttac gtaaaatcaa attttaaagg tgttatttaa ggtttattaa
    38041 attttgaatg taaaccatat ataaacatac gattgaaagg atagaaacga tgaaagtatc
    38101 aattgttgtc ccatgtttta atgaagaaaa aactgttcct cttttttttg atgaggttga
    38161 aaaattcaaa ggtgcccatg agttcgaata tttgtttatt gacgacggat caaaagacgg
    38221 tacgttagca gcaatcaaga agctagcctc agcttatcct tatgtccgct ttgtctcatt
    38281 ttccagaaat tttgggaaag aagcggctct gtacgctgga ttgcaagctt ctacaggaga
    38341 tttagtgacc gtgatggatg ttgatctgca agatccgccc gaactgcttc ctgagatgat
    38401 caaccggatc actacttctg atatcgattg tgtcggcacc cggcgttcga ctcgtgaagg
    38461 cgagcctgcg atacgcagtt tctttgcgaa aaaattctac cagttgatca ataaaatcag
    38521 cgatacagaa atggtcgatg gcgctcgtga tttccgcttg atgactcgtc aaatggtgga
    38581 tgccgtccta gaattaacag aatacaaccg attttcaaaa ggattattta gctgggtcgg
    38641 atttaaaaca gaatatattt cttatgaaaa ccgcgaacgt gtggctggcg aaacatcttg
    38701 gtccttctgg aaattattca actactcgat tgatggtatc gtgaacttct cggatgcacc
    38761 gttaacgatt gcttcattta tcggcgcttt gtcttgtgtc ggttctggta ttgcaatttt
    38821 gttcattatt ttgcgggcat tgttgtttgg tgatcctaca tcaggatggc cttccatggt
    38881 ctcgatcttc ctgttcattg gtggaatcca attgttatgt cttggaatca tcgggaaata
    38941 tattgggaaa atcttcttag aaacgaaaca gcgtcctgtt tatatcataa gagaaacaga
    39001 aaaagtgaag ccaaatgaat tgaagcctca ccaataatat acgtcaaatg aagtaaacat
    39061 ttttttaaat gaaaaaggac tgtgacaaaa gtcttgtcac agtccttttt tccgaataaa
    39121 tggtgttcca gaagtagctt cttcggaaat aagccgaaat ttcacaaaaa tttgagaagc
    39181 aattttcgta aattccttct tatttcttga agctgaccac ttctatcaca acctctttca
    39241 tatcttatta cttcttcatt gctgctttta gtgctgctgc taatgggctt tcttctggtt
    39301 cttctgcttg tgcatatttt ttcagcaagc gtcgttcttc atgtttggtc attttcttct
    39361 tctgctcttt tttatcaagc aatttttctg tcgttccatc gtatttacaa cggaaataag
    39421 caccgttttt accttcgatg atttccattt tccggtgaca ttgcgggcat cgatgattag
    39481 agacttttgg atcttttctt ctgcggtagt tgcaatctga attggtgcag acatagattt
    39541 tgccgtcacg tgtgtttttc tcgcgcagat tggccccaca ttctgggcat ttcttttggg
    39601 taatcgagaa atcttgatat tcttgtttgc ttgatttgat ctcattcacc aagcgttttg
    39661 tttctttttc gattccattc aagaaggttc ctgcttgttt ctttcctgca gcaatagctt
    39721 caaggtcttt ttcccagctt tctgtcaaat ctggtgttac taaagaggga tttaccaata
    39781 ctaataattg cttaccttta ggagatactt gcagtccttg aggggtacgt tccatcagtt
    39841 cagatttgat tagtttctcg ataatctctg cacgcgtagc tggggttccc agactgtatt
    39901 tttccatttg accaagcaag cttgcttctg acaatggttt aggcggtgca gatagttctt
    39961 ttttgatggc gaaatcggga ttgattctca ttcctttttc ccatttgact gtttccgtct
    40021 cagtttcagt ttcgcctttc catcctgctt ggacgacacg attttgccga aaaatgaatc
    40081 gttctttacc aaattcaacc gtaacttttg tttggctgac tttatggggt tgagcaaaca
    40141 gtcctaaaaa acgagagaca atcatttgat agatttttcg ctcttcatta cttaattttt
    40201 cataacgtgc tctgttttca gttggcaaaa gtgcatggtg atcggtcact ttactgtctt
    40261 ggaatacttt tgtctgttgg acgatggctc cgtttttcaa atactgcttg acctcaggag
    40321 caaagtcgct gactgcttgc aatcgttctt tcatcgtact tttcatgtca tttgtcagat
    40381 atttgctgtc tgtacgcgga taagtaacga tcttatgggt ctcatacaaa ctttgtacaa
    40441 gacttaatgt tttctttgca gaatagccat agcgttgatt ggcttctctt tggatctctg
    40501 ttaaatcata aggcaatggc gcagattccg tttttaattt ttcttggact tctgtgacag
    40561 ttcctttgct tgtactgatc aattgaacaa gtttttgtgc ttcttcttgt gtttttaaag
    40621 caaattgatt tttttgaagc attttggctt gttcttttcc agcttgcaac gtgatcgtga
    40681 aatacgtttg tggacggaac tgctcgattt tcttttcttg gtctctcact aatgctagtg
    40741 tcggtgtctg cacacggcct gctgataaat tatccgcgta cttcacagtt aaggctctgg
    40801 tcacattcaa tcctaccagc cagtccgctt ttgcccgggc aagagcagat tcatacaatg
    40861 catcatattc tttcgcaggt cttagttgct tgaatccagt tttgattgct ttatctgttt
    40921 gagaagagat ccataaccgc ttcactggtt tattgaaatg cacatactct aagatccagc
    40981 gagcaacaag ttccccttct cttccggcat ctgtcgcaat gactgcttct gagacatctt
    41041 ttcgctgagc cagctgcttg attgctttta attgatgtcc tgttttagga agtggtttga
    41101 ttcctagttt tttagggatc attggcaacg tgtccatttg ccagctctgc cattccttat
    41161 tcagatcttc cggcattttc aatcctaata aatgaccaag ggcccaagta acgatcacct
    41221 tgggtccttc ataataattt ttgtgctttt gattagcacc aagcaccttg cttaggtctt
    41281 tcgcaacgct tggtttttcc gctagaatta gttgtttcat aaaattcctt tctgctttct
    41341 actgtgatgg gctgatgatt tttcaacaac gcaagtccct tgtattgctg taaatcatcg
    41401 tcacactctt cacaccagcg ctcatctccg tcattccaaa aggcgctgag aaagttactt
    41461 tttgcattga tatattcata attttgatgg atttcgttcc attgtttttc gtacgcttgt
    41521 cgtgcttctt cgaaagactc gaatgtttct tcttcttcaa tatcttcttc ccagtcttca
    41581 aaaaaccacc aaggctcatt gtccccgaac atggtaatta cttgatacat actcgtgctc
    41641 cttctttcag cttttctaga ctcatcttag tcacttttca agaaagttgc aagaattttc
    41701 tcttataaaa gggtcattta atgaacatct taaaattgtt acaaatgaat ctgattgttt
    41761 aaggcaaagt agcgataaag ttattgatca tccaaaaata gctgtcttct ggatcgattg
    41821 ttggttcttt tgtaaagtat cctaaaaaac gtaaggaaag ataaccgtgg atcatactac
    41881 gaagttccct gcttcgatga actttttctt ctttcgttaa ttgaaaagcc tcaaatatct
    41941 gcaaaatgat ctgattcgtt tcatggattc catctaataa aacagcttca ttggtatgcg
    42001 gtacactgat cagtaactca tacactgctt gatttgaaaa agcaaaggat cgataagttt
    42061 ctgcatagat ctgcagtgct tcctgaccac tttttcccac aagagcccgt cgcagctggt
    42121 cattaagttc ctgcagaagt ttcgctgtta gcgctgtttt gacatcacga atatttttga
    42181 aatggttata cagcgaggga gccttgatat ccaagcgttt ggctaaatgc tgaaaggtta
    42241 tttgctgaac atccatctct tctgctagtt ctcgaaagca gtcgatgatt ttctcttgag
    42301 acagatttct ttttttcatt tccatgtttc ctctcataaa ataaatcctt cactctttca
    42361 gtttaccacg aaaaagaaag agaaaaaaga gactttcatt gataaactac atattgtagt
    42421 ttataataaa gagctgtaga tgaaaatttt cagaaagaag gagtctaatt ggctgcaaga
    42481 ataaaggaaa aattatttag gaaaagagaa acttgggaac aaaacttgtt tgtcttatgg
    42541 tttggaacat ttatggctgg tatcggattt agtttagtca tgccttttat gtcactttac
    42601 atagatacgt taggtaatta tagtacgtca caattgaact tttggagcgg attgactttt
    42661 tcatctactt tcttggtgac tacgcttatc tctccttggt ggggaaaatt agccgaccaa
    42721 aaaggaagaa aattgatgct gttgcgtgct tcattaggaa tggcaatcgt catttctttg
    42781 atgggttgcg tgactagtgt gtatcaatta gtcgcattgc gtctgcttca agggattttt
    42841 tctggttata tcagtaatgc cactgccctt gtcgcaactg ggacgccaaa agaaaaaagt
    42901 ggtcaagttc ttggaacctt agcaactggt tctgttacag gaacattact tggaccgctt
    42961 cttggcggga tctctgcttc agcgtttggc tatcgcccta cctttttcat tacagggatc
    43021 atcttatttt tcgtcttctt gttgagtctg tttttcgtcc atgaaaaatt tgtcccagtg
    43081 aaaaaagaaa acatggtttc tgccaaacag attttcaaag acttgaaata tccacacgtc
    43141 gtcatcggta tgttcgtcac aacgatgatc atccaagcat ccaacaattc cattagtcca
    43201 atcatcagtc tgtacattcg ccaattgctg catggacatg ggaatgttac gttgatcagc
    43261 ggaatcattg cttcgatacc tgggattgcc actttgatag ccgctccaag atttggtcgc
    43321 ttaggtgaca agattgggag tgaaagaata cttgcgattg gtttgggttt tgcgattctg
    43381 gtttatatcc caatggcatt cgtcacgaat gtttggcaat tagctgcctt acgttttttg
    43441 attggtatct ctgatgcttg tctgctccct gctgtccaag cattgatcac aaaatattcg
    43501 cctcatcaag ctgctggacg gatcttcagt tataaccaat ctttccaagc aactggtaac
    43561 gtagtagggc ctatgatcgg ctccagtgtt tccagtgcat ttggttatcg cggcgtcttt
    43621 ctttcgactt ctcttcttgt tttggtcaac ttcttgcttg tacgacacaa tacacaagag
    43681 atccatcagc aagaaactgc agaagaaaaa gcttcaggag tctccatagc ttcagggcac
    43741 tctccttctc atagacaata agcgtaaatg aaaaacactg cttgaacagt tgtcacagac
    43801 tgtccttgca gtgtttttgt ttaggcctgt tttttcaaga actcagataa gaggaataat
    43861 cccttttcca aggtttcttt tttacaacag taacctagtc gggcatgctt cggtaaatca
    43921 aaggcacttc ctggtactaa tagtactcct gtttcttcta ataaattgat acaaaatgtc
    43981 tggtcatctt cttcgatggc taatttaatg aaagaagtag agacgtaatt gggagcgaca
    44041 agttcaactt tcggttcatt ggctacccat tgttttaaaa tcacaaggtt ctctgtaatg
    44101 atctttttgt ttcgcgccaa tattttttct ttattcttca atgcatgtac agcaagatca
    44161 tctgacagga ctccgccgca aatcatcgtg tagtctcgat actttcgaaa aacttctgcc
    44221 aactcaggtc cagtagctgt ccagccaata cgaatacctg gaatggagta ggttttggat
    44281 agggaattgg ttgctatacc tttttcatag acatctacga tcgacaagaa ttcaccttta
    44341 tccgttaaag gcgcatatac ttcatctatc aatacatatg catctactgt acgagcaatt
    44401 tctgcgattt tttctaaagt tttccgatct agcaatgtgc ctgtaggatt gtttgcacta
    44461 ttcaaacaga tgatttttgt ttcaggtttg actaaggctt ctagctgttc taaatcaaac
    44521 tgccaatctt cttcttcctt caaatgaaca aagtcaacag cggcacctaa agatttgggg
    44581 atgtcatata attgctgata agtaggaagc atcgaaacaa catggtctcc tgcttccact
    44641 aacgcgtata gagctaaaag attagcacca gtcgctccat tcgtttgtaa gatgttttcc
    44701 ggatcgacgg tttgataaag agaagcgact tcttgtttga aaacatctga tccttcgatc
    44761 cagccatagt ccaatggttc gtttttgcgt ttttcgaaaa atttacttac gctagtccca
    44821 tctaagccaa cgagttcgtc taatgtcaat gcttcgatcg agctttgtgc aatatcatat
    44881 accgctttct tttcccattt atttagccat tcttctacac cgaaatttgc gattttcatg
    44941 tttctttccc ccttatatct tgatccagcc tacagcaagc aacccgattg aaagcagtgc
    45001 ggccacacaa atcagcccaa tcattacttt ttcttttccc gtaatttgat gtttggtttc
    45061 acgacaagcg atccagtaga agcaaaaacc aggaagatag gcaatactac ataaaaggac
    45121 ataagaaaat cctgctaaaa tgatgcccat caattcaaaa actacagcaa tcccaccgat
    45181 cactacttgt tttttgattc cttcttttaa agaaaactgg acttgataga tcccgacaag
    45241 tagataacaa atcaaaattg ccgctgtaca taaagaatag gcaaattgat aggcataatc
    45301 agtaactaag aaagtcaata agaatagatt agtcaagatt gcagtaacaa ctaacgaata
    45361 agttggggca ttttttttat tcgtttttcc ccaacctttc ggcaacagac cgtctcgagc
    45421 catcaacatc gttgtttctg ctgggagcat cgtccaagac aaccaagaac caatgatcga
    45481 aataatcaag ccgatattga tcagcgctgc tccccaattt cccacgattc cttttaaaat
    45541 atttgccata gcaggttgtg aaatagttgc taactcttct tgagataaga cgccgtatgg
    45601 caagatcgaa gcaaggatat agataaacaa taaacaaagc agaccaatga tcgatgcttg
    45661 ctgggcttct gaacgtttct tcgcgcggtt ggctaaaaca cttgcgcctt cgatccctac
    45721 aaagacccac atcataacca tcaaacaacc tttaatctgc aaccagatcg ttccttgctg
    45781 ccctgttcca ttcactagat tatcagatac gttaccccaa aaatcagcag tgaatatatt
    45841 cactttaaat gcgataaata caaatattaa aaataggaat aatggcacta atttacagat
    45901 cgtcacgatt gtattgatga cgctcgctcc ttcaactcca ttgtttacta ataaagtcag
    45961 accccaaata aaaatacttg caaataaaat agaaggaaca ttttgtccac ctttgaatat
    46021 cgggaaaaat tctcccatgg cgctcatcag catcgttgca aaagcaacat ttcccagcca
    46081 cgcagacaac cagtaacccc aaccactgat gaagccacct aatttaccaa atgaagcttc
    46141 cgcgtaaccg aaaataccac catctagatc tggacgcttt tctcctaaat gattcagcga
    46201 taataccaaa gctaaaatac caatcccaac gattatccat gaaaggatcg ctggccctgg
    46261 agctgcagaa ctagccagat cacttgtgat cccaaacaca ccgcttccga ttgacgaact
    46321 gacaaccaga gcaaccagtc cccatttgct aatacccgtc gatttctttt ccatttccat
    46381 caccctcact tataaaattg aacaaatcac ttgcaaaaga ttcccttcta ttttgcaact
    46441 gctcttgctt aacaaaaagc aactttattc atattattgg atattatgaa ctttttacac
    46501 aagtatttca agtataaaat gaataaaaat taattttatt gcaaaaaaac agctatgcta
    46561 tccaaaagaa aaggtgtttc ttttgttagc catagctgtt ttgattcatt taatatcgct
    46621 taaacgtatt gtaaaacttt tgtttcttga tatgcgtggt cgatcaaacc accacccaga
    46681 cattccattc catcataaaa tacaactgct tgtccaggag tgattgcacg tacaggttcg
    46741 tcgaatacga cttctgcacg attatcatcc aataagcgaa ctgtcacagg aacgtcttgc
    46801 tgacgataac ggaatttcgc tgtacacttg aattcttttg gcattggttc attcgtcgta
    46861 aagtggattt cacttgcatc caagcttgtg gcatacaatg caggatgatg gaacccttgc
    46921 cccacatata atgtgtttgt cgctagatct tttcctacaa caaaccaggg ttcttgcgaa
    46981 tctcctccgc caccgatccc tagtccttga cgttgaccaa tcgtataata catcaagcca
    47041 gcatgttgtc ctttgacttc accatcaaga gtgaccatat ttcctttttt tgctggcaga
    47101 tagttgctca aaaattgttt gaagttcttt tcaccaataa agcagattcc cgttgaatct
    47161 tttttcttag cagtcgcaag gcctgcgcgt tcagcaattg cccgtacttc tgatttttcc
    47221 atgccgccta gagggaacat cgtttttgaa agctgttctt gtgataattg gctaaggaag
    47281 taagtttgat ctttattatt gtctactcca cgaagcatgt ggaccacgcc gttttcatcg
    47341 cgagtgactt gcgcataatg tccagtagct acataatcgg cgccaagatc cattgcataa
    47401 tccaagaatg ctttgaattt gatttctttg ttgcacatca cgtctgggtt aggtgtacgt
    47461 cctgcgcggt attcggctag gaaatattca aatacacgat cccaatattc tttttcaaaa
    47521 ttcacagagt aataaggaat cccgatttga gcagctactt tcgctacatc cttataatct
    47581 tccgtggccg tgcatacacc gttttcgtcc gtgtcatccc aatttttcat gaatattccg
    47641 atcacgtcgt atccttgttc ctttaacaaa agtgctgtta cagatgagtc gacaccgccg
    47701 ctcatcccta cgacaacgcg tgttttgctg ttgtctgtca tgatatcacc atcatttcaa
    47761 ttagattctg aaacatctac gagttgaagg tcgcaacttt tcagtatttt acattatacg
    47821 tcatatttct gaaaatttca tcccttaatt atcaagtcgt aaaaatactg ttctactgga
    47881 cctattcagt acacaaacat gatagtcaac caaaaaatca ctcatctttt gagcattcga
    47941 tataaaaaat tggcatactc ataaaaaggg ccagcctttc ggcaataagg aataatttta
    48001 taaattgtga aaaactattt ttcaaaattg cttcttatcg cttaaagctg cacgtctcat
    48061 ttacaacctt ttctttttag atgttatgag ttgctcagat aagtgatttt gtttactcta
    48121 agaaaaaagg gaggcaaaag atacatgaat tactttatca gtgatatgca ctttttccat
    48181 gaacgcttat taggaaaaaa cgactttgct ccgcggctct ttgcaaatgt ggatgaaatg
    48241 aaccaacagt tgatcactag ctggaatgca gtaataaaag aaaccgacca tgtttatcat
    48301 ctaggcgacc ttgctatgca tccgcaatat gaaaaagcaa atgcagaaat tttggaactg
    48361 gtcactcaac tgcaaggaac aatccacttg atcaaaggga accatgacag tcgcgctttt
    48421 ttccgttatt tagaaaaaaa tgatcctagg cttactgacg gaagtcccaa attttatttt
    48481 tatgatgtag gtgccatcgt taaattcgac catcaacaat attacttgac tcattatccg
    48541 atgttgctcg ggcctaacga cactatccgc aatcttcatg gacatatcca tcattacagc
    48601 gtacctatcg agaacgatgt caatgtaggc gtagacgcac cggaaagaga actcttaaag
    48661 gaaaaactac catttggcac tccgttatct gagcaacaaa tcaaagaaat ctatgaagca
    48721 aagaggaagt acgataggtc gtgaaaaaac tgccctatat caagtattaa acaaataaag
    48781 ataattactc atatttttca tttgagtagg cttattgcca caggaacatt aggatcagaa
    48841 atggagttgt aacaagactt ctgtcacaac tccattccat actattcgtc tttttgctca
    48901 tggtgttctt ttaccagttt tttctgtaat tctttatcat aatttcgggc agctgacgtt
    48961 aaaaataaaa cgattccgca taataaaatc ccgattccgg aaaacaagaa cattttttct
    49021 acccctaaac gatcagctaa tggtcctgca aataataatc caattggacc tgccaggttc
    49081 atcaaagagt tgaagttccc taagacacga cctaaaacat tcgattcata actctgctgg
    49141 atcattgcca tcagcagtgt attgaaatat ggcgttgcac aacctgctcc tgcattcaag
    49201 ataagaaagt acaagaaccc ttgactatcg tttggaacca atccgcttgc accgatcgtc
    49261 aggcccataa tgatgtaggc tataataata ggtttcattc ggtctttcca tgttcctata
    49321 aaactgatca gtgcaccgcc taagagcatt ccgactgcgt agactacttc cactgctccc
    49381 gcctgtccaa cggaaccacc aaagtaatct agcgtcatca atggatacag actgattgct
    49441 ggcatgaaca acagcatcac aaatgcacca ttgatcgtta tgtaccataa ccctttatta
    49501 tccatcaatt gctgcagccc aaattttgca tctttaaaaa tagtaagcgg aacttccaat
    49561 atttcaggag aaacttttgg gatcttcaca aaaatcaata agccgacacc aaaaactgcc
    49621 cccaatacat ctaataaaat aaggtaattg acaggaacga ctgaaaacaa agccgcacct
    49681 agagctggcg caatgatgaa attcgctgat tggaccattc ctaactgtcc attcgtcctt
    49741 gttatgtgag aagatggaac gattgtcggc ataatcgact ggatcgttgg catttggaat
    49801 gtttgtgcga ccgaacgcat aaacaaagac acaaaaacta gccaaatcgg gaaggattct
    49861 gaaatcgttc cgactactgc taaaataagc gcgaaaatcg ctacaataat atccgtgaca
    49921 atcaaaagag cttttttatc ccagcgatcc actagcggtc ctacaaatgg gcttagtaaa
    49981 accatgggaa tcattcctag taaagtagca aaactcaaaa ttgtcgccga accggtttct
    50041 cttgtcaaat accagatgat cgcatattgg acgaccatac tagtgattcc tgacaaaaac
    50101 tgtcccgata aaaagaacaa ataatttctc ttccaattcg ttaaaacgac cgactgttca
    50161 ttttccataa acatccctct tttctttttc attttgtata gcgtttgcac aacaatcatt
    50221 atacgggatt cttttttcaa aaaatacctt tttccctaaa taacaatcgt ttcaatttaa
    50281 atttctttac ttagaaacaa taaatcagca aaatattaaa aataacaaca tgtgttaaat
    50341 ttaaaaataa aaaagacaac taatttgcgt cgtccttaca aagttagtca tctaacaata
    50401 ggccgtttca aaactagttg tctttttata tccacagatc attcgacttt ttcgaataac
    50461 ccacggctaa tcatcccatc catcagaaaa taaaatttat cttggctcat tttctgtttt
    50521 ggatctttga aaatattgac tgctttcaat ccatttgctg tagtgatcga cattttcaat
    50581 gtttgaatat cttttgatac agtgattttt aatttgaatc cttctttgct ttgcggcgtg
    50641 gcatctaatg gtcggatcaa ttgataattg cctccgtttg tttctgtaaa tccattatcg
    50701 cgcaatgtat atctttttgc atcaggatgc aaacgatagc taattggtgc gcccagtacc
    50761 gttgctgttg tttcaaatgc catgattttc acctcgataa aattagtttc tctttattat
    50821 aaaagtttat cctgattttt actaggtttt tcagttaagt tccctaaaaa atgaaggcgt
    50881 tctctcagac atctgttcat ataagaatga cttcactagt tattttttga cagcacgaac
    50941 taattgatca aggaaatact ggatgtcgtc ttcggtattt cccaaaccaa agctgatacg
    51001 gatggattct tttagtatcg gtgtatcttc tccatacatt gcttctaata cgtgtgaagg
    51061 ctcgatattt cctgctgtac aagctgaacc agtagaaata gcgaccccat taagatcaag
    51121 cttcatcagt agcaaatcat ttgcttcgcc aattaagcga agattcaaca catgagggag
    51181 tttattcgtt aaatcaccat ttacttgata agggatttcg tttgcttcta atgtatcaag
    51241 aatcaattgc tggaatcctt tatatttgtt gttacgtgct tcacgttctt caggtgacag
    51301 cagttcaact gcttttgcca taccccagat ccctgctaaa ttttctgtac ctgcacgccg
    51361 cttttcttcc tgctccccgc ctaaaagcaa tgcagggata tttactccgt ctcgtttata
    51421 taagaaaccg acgccttttg gcccattgat tttatgagca gagatactta ataaatcgat
    51481 tcctaattct tctggatgga tcgtttggtt tccgtacgct tgtaccgcat ctgtatggaa
    51541 ataagctgga tgatccgcta aaagttcacc gatttctttg attggcatca aatttccgat
    51601 ttcattgttc ccgtacatca cagagaccaa gatcgtatct tctcgtaacg cttttttgac
    51661 ctcttcgatc gacagattcc ctttttcatc tacaggcaga tatgtaacgt caaacccctt
    51721 tgtctctaaa tatttcatgg tattaagaac agcaggatgt tcgatagctg tagtgattag
    51781 atgtctgcct tctttttgac gcgagaacgc tgtttctaag atagctgtat tatccccttc
    51841 tgttccccca ctgttaaaga tgatctcgtg aggttttgct tgcagactgt ctgcaatgat
    51901 ttgtcttgct gtttccaatt tttcatgcgc gtggcgacca aatccatgta tgcttgaagg
    51961 atttccaaac gtgtcttgca tgattgccgt catcgcttca attacttgtg gatgcatggg
    52021 ggtagttgcc gcatgatcca gataaatagt tttcacttca ttcatccctt ccaaattatt
    52081 gctaggcaca ttctagcata aaatagcgaa caaaccaaag aagagctcat ttttgtctag
    52141 aggacgtttc atgggcatag aaaaaaggag ctgtgacaaa agtcttgtcg cagctccttg
    52201 aagctgctca cttctgtcac aacttctttc cctctttttt ttgtttattc gaattctttt
    52261 tcttccagac ggaataacgg actcatcggt gtattttcat ggatgcgttt gaccgcttca
    52321 cccattaatt ctgaacatgt aacgattgtt aagttttctg gatgacgatt ttcttctgtg
    52381 aaaacagaat cagtgataca aatatctttg attggtgcat tgtctaaaat acttttcgca
    52441 tgatcggaca ataatccatg tgacgcacag gcatatactt cttttgcgcc gttttctttt
    52501 aatacatttg ctgctcgtgc aagcgttgct cctgtgttca agatatcatc taccatgata
    52561 catgtctttc cttgaacatc accgatcaca taaccaccct cagttgaagc atcatctgca
    52621 tgatctacga tggctaatgt gctgttcaaa tattctgaaa gactacgtgc acgctgaaca
    52681 ccgctgtttt ttggcgaaac aactacgatg tcatctcccg tcagacctaa ttgacggtaa
    52741 taatgagcaa acaacggcat cgtaaataaa ttatctacag gaatatcaaa gaatccttgt
    52801 acttgaaccg tgtgcagatc taaagtcagt actcgagttg cccctgcttc ttcgatcaag
    52861 ttagcgatca atttcgcagt gatcggttca tgaggtttcg ccgtgcggtc ttgcctagca
    52921 tacccatagt aaggcaatac gacattgatc gttttcgcac ttgctcgctt cattgcatcg
    52981 atcatgatca gtagttccat atagtaatca ttgacaggtg cattcgttga ttgaacgata
    53041 tacacatgat cccctcgaat actttcttcg atattgatgg aaatctcgcc atcactaaat
    53101 tgcttcacga cgcttttacc taattctgtt ccgaacactt tcgcgatttt ttcagctaat
    53161 ggacggttgc cattcaaact aaaaatcttc aacgtgtcat cctgatactt attactcatg
    53221 tagtcctccc caaaacttct ctattcttcg atagtttaac atatttttcc aagattttct
    53281 gttaatttca gcaaattctt aaggaagtga aactgttccg ttactccaac tggcaggaat
    53341 gactttatga taatcgcccc agttgtcgtg tactttgtaa taacgttctc ctgtttcagt
    53401 ttcaaaataa gcgtacgctg tcacccaatg attgccatat gtactgccaa gtactttcaa
    53461 aataccaatc atcacaggtt tgccttcacg tatacgtttt gtagctcgct gccatgaacc
    53521 tagaagagtt gctcgtgcac gctcatgatt gccattcttc tttaaaaaac tagaaatccc
    53581 tgtactaacc tgaaaaggaa tcgtaggaag tcctagtggt tggatctggc tacgcaatgc
    53641 cttcgtcaaa accaacgaat cagccgtatt tttcttgcga atctcatttg gaagcatcca
    53701 ccccttacga tagtcttgat aataggcaag gagaactgct gccgcgtaag taccacaaag
    53761 atagcctgag ggggtagccc atttcttata cctcaaaaaa cgttcttctg gcaatcccac
    53821 ccagcaattt gcgagttcct ttcctgacct tttttcttcg atcaaaacgg gaatatcttc
    53881 ttttctgttg atccatctag ataactcgat tggctcttca ttgatcagct tccatttttt
    53941 acaagaaaga ccctcttgat aagtatattt tgctggagaa tattctaccg gatgtcctga
    54001 ttgatcgata atcacataac tttgatcttc aaaaaccaga gctaaaaaaa gcaatttact
    54061 ttttcttgac tgcagctgct gagtgctggc taatttttta atttttttat ctactttgaa
    54121 atataaaggt ttatccacta cttgattcag ttgggatcgc ctcctttttt tcattataag
    54181 tgaaggattg aaaaaaacaa atcagtttga ttgttcgaat tttcaatttt ttatgttcta
    54241 tttgttaatt ccttcatttt gtaagataat gggcacacga aacaaaaaag gagcgatcga
    54301 tgaatgaaca aagaacgatt gaaacaagat ttctatgaag cagtcaatga agaatggcaa
    54361 aaaacagcaa aaatccctgc agataaacca gctacaggcg gtttccaaga tttagttgac
    54421 gggatcgacc aacttttgat gggagaaaca gacgatctgc ttgcccatcc agaaaaagtg
    54481 tcttcagaaa aaatgaaaca tttccttgca tattatcaac tggcaaacga ttatgaccga
    54541 agaaatgagt tgcatgctca accgcttcaa cctataatcg aaaaagttga atcattgcag
    54601 tctttcgacg aattgaataa gcaatttcca gaatggctgt tggatacatt accattgcca
    54661 tttaatctgg atgtagatgc agatatgaag aatgcgcaaa cgaacgcctt ctttgcttat
    54721 ccgccttctt tattccttcc agataagacg tattatgcat cagaccatcc caacggtgca
    54781 caactaatag gcgttttctt tgatatggct gttcagttat tagagcttgc cggcaaagaa
    54841 aaaagtcagg cagaagcaat cacggaacaa gcagtgcaat ttgataaatt gattgctcct
    54901 catgtaaaaa gtgcagaaga aaacgcagat tacagcaaaa tgtataatcc gcaatctttt
    54961 gatacattta ttaaatacac agataaactg gacttgaaaa aacttgtcac gggtttgatt
    55021 gggacaactc cagataaagt gatcgtaact gatccagtgt acttcgatca tttgaatgac
    55081 atcctgacag aagctcattt tccgttattc aaaagctgga tgatcattaa aacagtacgt
    55141 tctcttagtg gttatctatc agaagaattc cgccaagtca gcggaatcta ttctcgcgta
    55201 ttgtctggaa cagacgaagc aatgcctcag aaaaaagcag cgtattacct tgcctctgga
    55261 caattcgatc aagtagttgg cgattactac ggaaaaaaat attttggcga agctgctaaa
    55321 aaagacgtag aacaaatggt gaaaaaaatg atcgccgttt ataaaaaacg tctagaagaa
    55381 aatgactggc ttggggatgc aacaaaagaa aaagccgtca taaaattaaa taacttaggt
    55441 atccaagtag gctaccccga taagatccca gcattattcg accagttcaa aacagtacct
    55501 gcagatcagg gtggtacttt gctttcaaat actcttgttt tcagcaaact cacattaaaa
    55561 gatcgtttca gcaaatggaa caaaccagtg gatcgttcag aatgggaaat gagtgctgac
    55621 acggtcaatg cttattatca tccatttcgg aatgtgatcg tctttccggc agcgatcctt
    55681 caagcaccat tttacagtct agaacaatca agctctgcta attatggtgg aatcggagct
    55741 gttatcgctc atgagatttc ccatgcattt gataataacg gcgctttatt tgacgaatat
    55801 ggcaacttga acaactggtg gacagaagaa gatcttacac aatttcaaaa caaagcacaa
    55861 gcaatgatcg aagaattcga tggtctgcct tttgctgacg gaaaagtaaa cggaaaatta
    55921 accgtttcgg aaaatattgc tgatgcaggc ggtctaagct gtgcattgga agcagcaaaa
    55981 acggaatcag atctttctat cgaggaattc tttgtcaatt gggcaacgat ttggcgtaca
    56041 aaagcaaaaa aagaatacca acaactttta ctgcaaatcg atgtccatgc ccctgctaaa
    56101 ctccgagcaa acgtgcaacc aaaaaacctt gacgaattct atgaaacatt tggtatcacg
    56161 gaacaagacg ctatgtatct agcgccagaa aaaagagtgc gaatctggta agaggttgtg
    56221 ccagaagtgg tcagcttcaa gaaataagaa ggaatttggg aaaatcgctt ctcaaatttt
    56281 tgtgacattt cggcttattt ttcgaagaag ttacttctgg aataccatgt attagaggtt
    56341 gtgaaaaagc tgcttagttt caagtagtaa gaaaaatttt gtgaaaatgg ctttccacat
    56401 ttttgcataa atttgactta ctaccgagaa actggctttt gaacaccgtt tatcgagagg
    56461 ttgtgaaatc agcgtcctga cctgagtatt agagaaaaaa acggaaaaat agttcctcat
    56521 attttgccgt ttgttgagct aataccgagg gtcagctgat tgaacaccgt ttagtggaga
    56581 gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaatcaaaa gcagcaagga
    56641 gaacctcatc tataaaaaga tagaaggaag tgagacatct attggtctca tttccttcta
    56701 tttttacttt cctcagtcaa ttgttttaca tcataaaagg agaaattcca cttatttttt
    56761 tcgctgtttc atgacataaa caactacacc gattaacaaa attcctccag ctaatgctag
    56821 caacgggtta ttaaattctc ccgtttttgg gagtccttga gtgccatttt tttcactttc
    56881 ttttgctgtc ctatccggat gagaaggttt tccatttggt tttccatttg gtgattcatt
    56941 tcctggattt tctctttctg gtttgtcatt tcccgggggc acatcggcag tttctcgcac
    57001 agtgatcgct ttttgctggg taacagattc tgtatgagca gaccattttt ccccatcaaa
    57061 tatttccgaa cgataagtga cttctagcgt atattctcct tttgaaggtg ctgaaaaaat
    57121 agcggtatga ttagaaaatt gagcacttga ctgtgtttca acgatttgga tcttttctgg
    57181 gaaataacgc atatcttcaa agacaggcgt tccttgattt ggtgtgaaag ttgacacaaa
    57241 aactgtaaaa ggcttcagtt caatgatatt tccttctgca ttctccgcca caccatttgc
    57301 ttcttgtata ataatcggat tattttgttc atttggtttt gtctgatttg gttcttgtac
    57361 gattgcatac tctggatatt ccgaagacgg tgaggtttct ccgtcaaaaa tagcacctac
    57421 agataatgga agaccatcca ctctttcctt ttctgtttga cgattccccg gtttaatctg
    57481 catcagtcca tgaacatgga tacttccatt ttcatagcgc gtaacaggct gctgaccttc
    57541 tttcgaacat gtatttagag actcgatcca atcttttgat atggtatgtc caagagtatt
    57601 ttctgattgt ttctctcgtt cattccaata gtagttcaac gagtggtaga tttgatcttc
    57661 gttttgattc aatgggcgga tctgctctct catctcaaga ttctctgttc ggaaagataa
    57721 gataccagaa gcatggtagt ctgtgtttcc tgctgaagag gtataaagag ccacattata
    57781 tgaaccgtta ttaaaacttg tactgctatt gacgataata tctgttccgc tattggaatc
    57841 gattcctttc gcaagatttt cgaaagacaa actattgatc aattcatgag ggcccgttaa
    57901 agaagaaccg cccattttga atccattacc gttaccttct ccctcaattc ctggtaccca
    57961 gccgtttcga taagcaactg agtttttgat gattactttt ccgatcgaac ctgtttcatt
    58021 tttagcaaaa aggtcccaac cgtcatctgc attatgatac gcgatacaac cgtcaaatac
    58081 atttccttct cctattgtca gtttggccgc aaatccatcg gcatcttcaa atcctggatc
    58141 ggcattttca aaagatgtac agttcttgac taagttatgc gcgggccatg cttcgaaagg
    58201 atcatttgag ctaccgctga tttggattcc tgtatttcca ttccgataag catgaatctc
    58261 ttcgatcaaa ttatgatttc cagaaatctg taatcctttt tgcatagctg cactatttgt
    58321 aatgtcaaac cctcgtaaac cccagtagtg actgaacagt gtcatcccat ttccagttcc
    58381 tttaaaatca agtacaggac gcttgttttc tggatttttc tctgcaatca actgaatcgg
    58441 gttttctctt gtcccgttaa cgccttttgg aatcgtcaat cctctttcga aggaataagt
    58501 tccaggcgcc atgacgattg tctgcccagc atatgcgtat ctcaatgctt gtgtcagaga
    58561 taatggattt tgtctgcttc cgtttcctgc atctgaccct tctggtgtta cataaactgt
    58621 cgaatattta ggtttacggt agtccactgt atgaagaatc tcttttgttt cataactagt
    58681 catctctacg tattcttctg gcggatagtt tttatctggc gtaaaactga aagtaaattc
    58741 gttcgttcct tccatcagtt ttgtctgata atcaaattcc cgattcgcag tgatagccaa
    58801 gttttttgcg ttcttaagca cctctcctgt tgctttattt ttaattgtca ataatccgtc
    58861 agcatttgct ctaaaggaca tagtatgatc ttggtttcct gaatgattac tagaggtcac
    58921 agtgtaaatc ggatcaatca agcgagtagg tctttcttgc ccaggaggat ctgtcgcagg
    58981 atctgagtaa gcaacttcga ttccttctac cttgatcgtc atattccttg ctgcagcaaa
    59041 tcctgcataa ataaccgatt catctgaagc gtataatttt tcccagtcat acatgatgtc
    59101 agaagccatt tcttctcctg tttcaggatc gtaataatat gcctctaaac cagtattcgt
    59161 ttttttcatc tctacgtcca gtttagtaat cgagttcgtg gccggaaaca gattcccatg
    59221 accgtttccg aatatattat aagaacctgt ttctttttcc aagaaccctg ctgaagtctc
    59281 taaaggtgtt gtttccactg agacactccc aggagctggc gcagcttgcg gatcttcaga
    59341 tgatattcct gtgattgccc gtgtccccaa tccaagtcgc atcgtatatt tgctgccgct
    59401 cgtgttgacc acttcttgtc tatccgagtc ccagacatat tcgatccgac tgcctagtat
    59461 ggcaaatgaa tttgaataaa atgcctgccc aaacaaatga ttttttggta ctgcgtctct
    59521 taccattaaa gcaaatcctt cttgggcatt cgtataggtc caagattcta tttcgatcgt
    59581 cgcccgcatc ctaaagttga tgtctttggg gattgcttga taataatagg tcattccatc
    59641 tgatccgctt gactggaatt ttccgccgtt attcgtggaa gagagccgca cgccgtcttc
    59701 taatggttca atggcatacc tactagcact tgtgctttct ccgaaaacaa cccctttcca
    59761 ctcttcattt gcttcttcag attgcttttt tgtcttttca ttttctgatt ctttcatcat
    59821 aggatcattt ccttcttccg tatccatatc ttcactagtt cttggtattt cctccattgt
    59881 tgacatccct tcaatgttca cgtgtgcatt ttcttcgttc acagactgtt gttcgttagc
    59941 agcaacttca acgacagaaa ttaaaaccgg ggtactgagc ataaggagat tcatcattag
    60001 cgcactgtat tttatacgtt tttttaacaa gtgtattcct cccgtaacaa attagaaagc
    60061 gtttacttaa agtattacct aatttatttt tactgtcaat aaaaaacaaa attataataa
    60121 gtgtatttta taaaaagaaa aaatataact taagttgttg ataataaaga gatttattaa
    60181 cgtaaaaaga ataagttttt
//
LOCUS       DAZKOR010000006        57321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000006 DAZKOR010000000
VERSION     DAZKOR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.006"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(525..1028)
                     /locus_tag="ACG8ZM_000289"
     CDS             complement(525..1028)
                     /locus_tag="ACG8ZM_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0491284.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1387..2412
                     /locus_tag="ACG8ZM_000290"
     CDS             1387..2412
                     /locus_tag="ACG8ZM_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0491285.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2735..3613
                     /locus_tag="ACG8ZM_000291"
     CDS             2735..3613
                     /locus_tag="ACG8ZM_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0491286.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4089..4205
                     /locus_tag="ACG8ZM_000292"
     CDS             4089..4205
                     /locus_tag="ACG8ZM_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0491287.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5133..5471
                     /locus_tag="ACG8ZM_000293"
     CDS             5133..5471
                     /locus_tag="ACG8ZM_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491288.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5565..5795
                     /locus_tag="ACG8ZM_000294"
     CDS             5565..5795
                     /locus_tag="ACG8ZM_000294"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0491289.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6195..6566
                     /locus_tag="ACG8ZM_000295"
     CDS             6195..6566
                     /locus_tag="ACG8ZM_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0491290.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6840..7532)
                     /locus_tag="ACG8ZM_000296"
     CDS             complement(6840..7532)
                     /locus_tag="ACG8ZM_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0491291.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7535..8320)
                     /locus_tag="ACG8ZM_000297"
     CDS             complement(7535..8320)
                     /locus_tag="ACG8ZM_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0491292.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8557..9957
                     /gene="lacG"
                     /locus_tag="ACG8ZM_000298"
     CDS             8557..9957
                     /gene="lacG"
                     /locus_tag="ACG8ZM_000298"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0491293.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9982..10299
                     /locus_tag="ACG8ZM_000299"
     CDS             9982..10299
                     /locus_tag="ACG8ZM_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0491294.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10312..11985
                     /locus_tag="ACG8ZM_000300"
     CDS             10312..11985
                     /locus_tag="ACG8ZM_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0491295.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11999..13630
                     /locus_tag="ACG8ZM_000301"
     CDS             11999..13630
                     /locus_tag="ACG8ZM_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0491296.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14121..15686)
                     /gene="guaA"
                     /locus_tag="ACG8ZM_000302"
     CDS             complement(14121..15686)
                     /gene="guaA"
                     /locus_tag="ACG8ZM_000302"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0491297.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15745..16668)
                     /gene="coaA"
                     /locus_tag="ACG8ZM_000303"
     CDS             complement(15745..16668)
                     /gene="coaA"
                     /locus_tag="ACG8ZM_000303"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0491298.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16864..17469
                     /locus_tag="ACG8ZM_000304"
     CDS             16864..17469
                     /locus_tag="ACG8ZM_000304"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0491299.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17777..18793
                     /locus_tag="ACG8ZM_000305"
     CDS             17777..18793
                     /locus_tag="ACG8ZM_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0491300.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18934..20235
                     /locus_tag="ACG8ZM_000306"
     CDS             18934..20235
                     /locus_tag="ACG8ZM_000306"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0491301.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20281..20943
                     /gene="deoC"
                     /locus_tag="ACG8ZM_000307"
     CDS             20281..20943
                     /gene="deoC"
                     /locus_tag="ACG8ZM_000307"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0491302.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20964..21362
                     /locus_tag="ACG8ZM_000308"
     CDS             20964..21362
                     /locus_tag="ACG8ZM_000308"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0491303.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21428..22510
                     /locus_tag="ACG8ZM_000309"
     CDS             21428..22510
                     /locus_tag="ACG8ZM_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0491304.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22722..24284
                     /locus_tag="ACG8ZM_000310"
     CDS             22722..24284
                     /locus_tag="ACG8ZM_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491305.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24277..25410
                     /locus_tag="ACG8ZM_000311"
     CDS             24277..25410
                     /locus_tag="ACG8ZM_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0491306.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25410..26366
                     /locus_tag="ACG8ZM_000312"
     CDS             25410..26366
                     /locus_tag="ACG8ZM_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0491307.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26465..27631
                     /gene="deoB"
                     /locus_tag="ACG8ZM_000313"
     CDS             26465..27631
                     /gene="deoB"
                     /locus_tag="ACG8ZM_000313"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0491308.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27655..28473
                     /locus_tag="ACG8ZM_000314"
     CDS             27655..28473
                     /locus_tag="ACG8ZM_000314"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0491309.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28490..29197
                     /gene="deoD"
                     /locus_tag="ACG8ZM_000315"
     CDS             28490..29197
                     /gene="deoD"
                     /locus_tag="ACG8ZM_000315"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0491310.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29495..30181)
                     /gene="gpmA"
                     /locus_tag="ACG8ZM_000316"
     CDS             complement(29495..30181)
                     /gene="gpmA"
                     /locus_tag="ACG8ZM_000316"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0491311.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30353..30934)
                     /locus_tag="ACG8ZM_000317"
     CDS             complement(30353..30934)
                     /locus_tag="ACG8ZM_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0491312.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(31067..31750)
                     /gene="rpiA"
                     /locus_tag="ACG8ZM_000318"
     CDS             complement(31067..31750)
                     /gene="rpiA"
                     /locus_tag="ACG8ZM_000318"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0491313.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            32069..32482
                     /gene="rpsL"
                     /locus_tag="ACG8ZM_000319"
     CDS             32069..32482
                     /gene="rpsL"
                     /locus_tag="ACG8ZM_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0491314.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32566..33036
                     /gene="rpsG"
                     /locus_tag="ACG8ZM_000320"
     CDS             32566..33036
                     /gene="rpsG"
                     /locus_tag="ACG8ZM_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0491315.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33130..35217
                     /gene="fusA"
                     /locus_tag="ACG8ZM_000321"
     CDS             33130..35217
                     /gene="fusA"
                     /locus_tag="ACG8ZM_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0491316.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35389..36576
                     /gene="tuf"
                     /locus_tag="ACG8ZM_000322"
     CDS             35389..36576
                     /gene="tuf"
                     /locus_tag="ACG8ZM_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0491317.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36983..37291
                     /gene="rpsJ"
                     /locus_tag="ACG8ZM_000323"
     CDS             36983..37291
                     /gene="rpsJ"
                     /locus_tag="ACG8ZM_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0491318.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37322..37951
                     /gene="rplC"
                     /locus_tag="ACG8ZM_000324"
     CDS             37322..37951
                     /gene="rplC"
                     /locus_tag="ACG8ZM_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0491319.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37979..38602
                     /gene="rplD"
                     /locus_tag="ACG8ZM_000325"
     CDS             37979..38602
                     /gene="rplD"
                     /locus_tag="ACG8ZM_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0491320.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38602..38892
                     /gene="rplW"
                     /locus_tag="ACG8ZM_000326"
     CDS             38602..38892
                     /gene="rplW"
                     /locus_tag="ACG8ZM_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0491321.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38931..39764
                     /gene="rplB"
                     /locus_tag="ACG8ZM_000327"
     CDS             38931..39764
                     /gene="rplB"
                     /locus_tag="ACG8ZM_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0491322.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39807..40085
                     /gene="rpsS"
                     /locus_tag="ACG8ZM_000328"
     CDS             39807..40085
                     /gene="rpsS"
                     /locus_tag="ACG8ZM_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0491323.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            40108..40455
                     /gene="rplV"
                     /locus_tag="ACG8ZM_000329"
     CDS             40108..40455
                     /gene="rplV"
                     /locus_tag="ACG8ZM_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0491324.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40469..41125
                     /gene="rpsC"
                     /locus_tag="ACG8ZM_000330"
     CDS             40469..41125
                     /gene="rpsC"
                     /locus_tag="ACG8ZM_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0491325.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            41128..41562
                     /gene="rplP"
                     /locus_tag="ACG8ZM_000331"
     CDS             41128..41562
                     /gene="rplP"
                     /locus_tag="ACG8ZM_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0491326.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41552..41740
                     /gene="rpmC"
                     /locus_tag="ACG8ZM_000332"
     CDS             41552..41740
                     /gene="rpmC"
                     /locus_tag="ACG8ZM_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0491327.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41765..42031
                     /gene="rpsQ"
                     /locus_tag="ACG8ZM_000333"
     CDS             41765..42031
                     /gene="rpsQ"
                     /locus_tag="ACG8ZM_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0491328.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            42089..42457
                     /gene="rplN"
                     /locus_tag="ACG8ZM_000334"
     CDS             42089..42457
                     /gene="rplN"
                     /locus_tag="ACG8ZM_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0491329.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42494..42802
                     /gene="rplX"
                     /locus_tag="ACG8ZM_000335"
     CDS             42494..42802
                     /gene="rplX"
                     /locus_tag="ACG8ZM_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0491330.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42830..43369
                     /gene="rplE"
                     /locus_tag="ACG8ZM_000336"
     CDS             42830..43369
                     /gene="rplE"
                     /locus_tag="ACG8ZM_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0491331.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43388..43573
                     /locus_tag="ACG8ZM_000337"
     CDS             43388..43573
                     /locus_tag="ACG8ZM_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0491332.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43609..44007
                     /gene="rpsH"
                     /locus_tag="ACG8ZM_000338"
     CDS             43609..44007
                     /gene="rpsH"
                     /locus_tag="ACG8ZM_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0491333.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            44039..44575
                     /gene="rplF"
                     /locus_tag="ACG8ZM_000339"
     CDS             44039..44575
                     /gene="rplF"
                     /locus_tag="ACG8ZM_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0491334.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44736..45092
                     /gene="rplR"
                     /locus_tag="ACG8ZM_000340"
     CDS             44736..45092
                     /gene="rplR"
                     /locus_tag="ACG8ZM_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0491335.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            45113..45613
                     /gene="rpsE"
                     /locus_tag="ACG8ZM_000341"
     CDS             45113..45613
                     /gene="rpsE"
                     /locus_tag="ACG8ZM_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0491336.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45628..45807
                     /gene="rpmD"
                     /locus_tag="ACG8ZM_000342"
     CDS             45628..45807
                     /gene="rpmD"
                     /locus_tag="ACG8ZM_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0491337.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45851..46291
                     /gene="rplO"
                     /locus_tag="ACG8ZM_000343"
     CDS             45851..46291
                     /gene="rplO"
                     /locus_tag="ACG8ZM_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0491338.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46291..47586
                     /gene="secY"
                     /locus_tag="ACG8ZM_000344"
     CDS             46291..47586
                     /gene="secY"
                     /locus_tag="ACG8ZM_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0491339.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47648..48295
                     /locus_tag="ACG8ZM_000345"
     CDS             47648..48295
                     /locus_tag="ACG8ZM_000345"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0491340.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48486..48704
                     /gene="infA"
                     /locus_tag="ACG8ZM_000346"
     CDS             48486..48704
                     /gene="infA"
                     /locus_tag="ACG8ZM_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0491341.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48737..48853
                     /gene="rpmJ"
                     /locus_tag="ACG8ZM_000347"
     CDS             48737..48853
                     /gene="rpmJ"
                     /locus_tag="ACG8ZM_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0491342.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48871..49236
                     /gene="rpsM"
                     /locus_tag="ACG8ZM_000348"
     CDS             48871..49236
                     /gene="rpsM"
                     /locus_tag="ACG8ZM_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0491343.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49264..49653
                     /gene="rpsK"
                     /locus_tag="ACG8ZM_000349"
     CDS             49264..49653
                     /gene="rpsK"
                     /locus_tag="ACG8ZM_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0491344.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49733..50671
                     /locus_tag="ACG8ZM_000350"
     CDS             49733..50671
                     /locus_tag="ACG8ZM_000350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0491345.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50699..51079
                     /gene="rplQ"
                     /locus_tag="ACG8ZM_000351"
     CDS             50699..51079
                     /gene="rplQ"
                     /locus_tag="ACG8ZM_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0491346.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51351..52190
                     /locus_tag="ACG8ZM_000352"
     CDS             51351..52190
                     /locus_tag="ACG8ZM_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0491347.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            52166..53035
                     /locus_tag="ACG8ZM_000353"
     CDS             52166..53035
                     /locus_tag="ACG8ZM_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0491348.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            53025..53822
                     /locus_tag="ACG8ZM_000354"
     CDS             53025..53822
                     /locus_tag="ACG8ZM_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0491349.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            54025..54771
                     /gene="truA"
                     /locus_tag="ACG8ZM_000355"
     CDS             54025..54771
                     /gene="truA"
                     /locus_tag="ACG8ZM_000355"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0491350.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            55435..55629
                     /locus_tag="ACG8ZM_000356"
                     /pseudo
     CDS             55435..55629
                     /locus_tag="ACG8ZM_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(55817..56593)
                     /locus_tag="ACG8ZM_000357"
     CDS             complement(55817..56593)
                     /locus_tag="ACG8ZM_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0491351.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            56716..57123
                     /locus_tag="ACG8ZM_000358"
     CDS             56716..57123
                     /locus_tag="ACG8ZM_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFQ0491352.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT        18350 a        10215 c        12196 g        16560 t
ORIGIN      
        1 aataacatat ctcattttac acttttgtta ttttatactt aaaaaataac gacacagaaa
       61 ataccgtgtc tcagatttct ctactacttc taaaaactac tgtttttttc ctttgaaatg
      121 ccttcaaaaa gtaggatcag gcagaatata ctggcagaat caaggagaag ggagcctaaa
      181 taaggagaaa aaccatagaa aatataaagt aattacttat aaaacaagct ttttatagaa
      241 aaataatata cctgagtagg gtataaatta agacggaatt cctttctaga ttgagatatt
      301 gactaggaag aatgttaaga ttttaagcct ttaagctaaa aaaaaaccct ttttgttaaa
      361 taaggggaat atagcctgtc agtttcaaac tgcaagtcgt cctgctgcaa taatatagta
      421 gattagagat gaaaatagat ttttttaaac caatgactga ataaaaagac cgtcatagat
      481 acacattgga atgtgtctat tacggtcttt ttttacgaac gattttattg aagacggaca
      541 gttcgggtca aaaataaaaa agctacgatc agaaataaga ataaaatgta tcccaaaata
      601 atggctagac ggtctaactt atttgtcgat agatgtttgt ttagaagttg ataggcccat
      661 aaacctatca gtcctcctgt tgtattagtt atcaaatcag tgatgtccgc tacgccaata
      721 ccgaatagga attgaatcaa ttctgccaag aagcttacaa ggaaaataat ccctaatttt
      781 ttagaaaaag aaagtctctt gaaattgaca ttcaaaagca ccccaaaagg aaaaaagaca
      841 atcaagttat aagtggtctc acgtaatacc gtcgtatttt caaaagaaaa agtagaaaaa
      901 gggacgagat tgacattgct gtaagaataa gtgagaaatt gtggaagatt aaatgatagc
      961 ttgaataaaa tcaaccataa tagaaagaat aaataaatcc ctaataaaaa ttttgataca
     1021 taccgcatag acatctcctc catttaaaaa gtataccatc gaaaaccgct gaaaaatcat
     1081 aaaaaagtat gaagtttaag gaccaacgta gttatcgtac gaaaattaga agcttttttt
     1141 tgttgggaat ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta
     1201 tcttttttct gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt
     1261 ttactttttg atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct
     1321 gacaaacggg attggcattt catcttggtt cagctttttt tccaattact aggaaggagg
     1381 gatagattgt tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc
     1441 atgaatctga tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt
     1501 ttgatttcaa gtttttcatc attagggatc tttacttttt tctattatca aaacataccg
     1561 atgaaatatt tgctgaaacg tatgtttctg atcggttgcg gactgttggt tgctttcgtt
     1621 ttggggatct tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct
     1681 ttttccagca gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt
     1741 gcgatgcttt ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc
     1801 tcactttatt tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta
     1861 ctggattcac ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa
     1921 gagcctttag tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat
     1981 gtcagtcacg gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt
     2041 ttgctagcag aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg
     2101 atgggtgggt tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc
     2161 atttttctga tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca
     2221 gttgctaata tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca
     2281 tacttgattt ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt
     2341 ttaggtgttg cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa
     2401 aaattagaat agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac
     2461 aacctctaat tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa
     2521 aatgtggaaa ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat
     2581 gatggtaact gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat
     2641 attctttctt catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata
     2701 ccatatggtg tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg
     2761 aaatttgatg aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc
     2821 caaagtcgtt atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga
     2881 gcaatatagt catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga
     2941 caaagtctat gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca
     3001 aagttggaaa aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat
     3061 gaatgctttt actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga
     3121 ccgtggtttt tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga
     3181 gtacgaaatg acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg
     3241 cctttctctt catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta
     3301 cgaaagtgcc attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta
     3361 cacagatact tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat
     3421 ctgtcttttt gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt
     3481 cccttggatc tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt
     3541 acagcgacaa gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat
     3601 tcttacttca taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag
     3661 aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg
     3721 gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc
     3781 ttggagttaa acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg
     3841 ttcaataggt aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt
     3901 ttaggtagct aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag
     3961 tctgatcgac tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac
     4021 aaatcgtaat tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg
     4081 tcgtccatat ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata
     4141 tcaaaacacg aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa
     4201 ggtaagaaga aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca
     4261 ataatcggtt tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat
     4321 atgataaatt ggtagaatct ttcttttcaa attgatacca ttccctaatt ccagttatat
     4381 agttaactac agaaccacaa agcacgtacg tttataaaag tattatctgt agttattttt
     4441 caattccctt tcaacctctt attttgacca gtagtattga tcactttacc ttttaacacc
     4501 tttgttcgtt tttgaaacat ttttagtcaa gaatggaaac gtaactacat aatcaaaggt
     4561 tttgcgaaat atttcatgaa atatttcatg tagaagcgag caatacggtt gcaaataacc
     4621 attatcgttt aataagcagt tacgaaagct cccaatatct ctaacgttca gctcactcca
     4681 tttcttttct tcaaaaaaat cagtgattcg tatctctttc tatttgtcga aaaagttaac
     4741 tttaaaaagt aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac tattgctaga
     4801 ataacatcaa ggaataataa gttttggttt tcagataata gtcgataagt tgtgtattgg
     4861 aattttagat acagtaataa agtatttttc atgaaatttt gtgagaatca ctattacaag
     4921 tatctttaat aaaattaatg gttaaggagg aacttaaagt agaatgtaaa tgaatagttg
     4981 tagtcagtcg attgtgatac tagactcaaa gttgcaggaa taagtttttc aatttgtgat
     5041 tggaagataa aaaatgaaaa agaagggctt ttttttatac tttgttgatt gtagtgttta
     5101 ttttccctct gtttagccaa gtaacatttt ctatgactca accagtttca agtgaggaac
     5161 atgataacct ttttcgtgct acagaagagt ttaagaaata tcaggaaaat attgttccgg
     5221 agactccatt agctagtgag ccaattactg atattgatgg aaaccaaata ggatggtata
     5281 tacagtacga agttatggtg gtaaataatg actcaatagt aaaaaataca gtaggcttag
     5341 agctaacacc gattgaaaag gtgagttcat tgttaacgta tatttatact gatgaaagtg
     5401 acgagactga gatcatttta acggattact caaaaattgc tactgataaa aagtatatat
     5461 tgtggatgta gacactagtg aaatacttta tgatatagaa attgatgaaa atttactaga
     5521 tgtagcacaa gaaatcgatc atcaaaagca ggaagctatt cgatatgctg gtagaaaagg
     5581 gaaaagaaaa tatttattac gtgaatgtag caaaagttag agaagatgaa aatgagtgga
     5641 aagaattcaa gagtcgctac tcaattaact cgactccgac atttacagtt tacagagaag
     5701 gatcgataga aaaaactgtt ttttggacta aagaaagtgg aatgtcttta gctgaagtag
     5761 aagagttttt ggattatgtt agtatgcaac agtaagggag ttcttattat gaaaaatttt
     5821 gacacatatt atgttattgt tttttcgata tctttgctgt taatattact aactttaatg
     5881 cagtataaag agtatccaga gtattttatt ttaacttttg tatatttagt aagtagctac
     5941 acacttctat ataaattcag aagttcttct agagtttcta aagtttgttt cttaattagc
     6001 ttattgattc tataatattt ttgatttata taggaaatta aaaatattat agaatcaaca
     6061 ctcagagaac gggttggttt ttaatagatt taataagtac tgataatgaa atttctcctg
     6121 gttgggaaaa cgaagttggt taccctgtag aagaattttt tcaagattac tttgcggagt
     6181 atgccgatag gataatgaga gattacgtaa cagataaaac tcaagaagtc atcaatcaat
     6241 cccagtcatt aaatgaatta tctgataagg cgaaagaaga agcagttgcc attatttctt
     6301 cagggattag tgactatatt gatatgagtg gtgaaatcaa ttatggcgat aaagacaaaa
     6361 ttgtacgcat gataaaagct tttatcaaaa cttttgatgt gaaggcagat cttaagttta
     6421 atgatactta cctagtagga aaattaatta atatccttgg gacagagaaa gatacggaaa
     6481 cgtttatttc ctctttgtct atgttgttta cagataagca tttttcaact cagcttggtg
     6541 gagaggaact agtggaaatc ttttaaccat tacgcccagc attaataaaa gtatatcaaa
     6601 aaagtactcc tgatgatttt taagattggt ttgagaaata atgtgaaaac actattttta
     6661 atgttaatta tagatagaga tgaacgcgtc atatggttaa aagaatgaaa aagtcaaaga
     6721 tacactgcta tatttcacta aggatatagc agtgtatctt tcatgtcgtt tcactggcag
     6781 ttttttataa aaggaggtgt gcatttcagt tgcttttctt tggtagttag ttgaatcgtt
     6841 taagaattaa cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa atctttgatg
     6901 gttccatctt tatttagctg tgcctgttca aatactgttt tgtttttacc gaatggatca
     6961 attgcaataa ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga gtaagcatct
     7021 tcgataacaa gacaatcagc cggagctaag ctcagtttgt ctgccgcttt aagaaaaata
     7081 tccggttgcg gtttcccagg gaaagagccg tcgtcataga caatcttgtc ccagtcaaac
     7141 cagcgggcta aatcgaaaat atcaaaataa aaagcaacat tctctttgac agtagccgtg
     7201 gcaatcgtta atggaatctg ttggtcagtc agctggtcaa gagtcgcttc aagccctttt
     7261 gtcaactgaa gctcgttttc attttgcaag caaagcatac gataatagct ttctttttct
     7321 tctgacaata cggcgatctc agaggcggtc aaatgctctg aaatgaaatg tttcaaaatc
     7381 tccgtattcg tccgaccatg aatatttttt aaaatatcct cgtctgataa accatctttt
     7441 gtatgatcat gtatcatatg cagccaagca gcttcatgca gatgagagtc caaaaacatt
     7501 gttccgttga aatcaaatat aatccctttc atagttacgc tcctatagtg gtattttcgg
     7561 ttgaatcagc aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc tcccgcgaga
     7621 tctggcgatc cgtaatgagc agatcgaaat catgcaaatt ataaaacaca taaaaatcat
     7681 ctcgattgaa ttttttatta tcaacgagta gatatttctt acgggaatta tttagagcga
     7741 tctgctgtgc ttctccttct tcggtactgt aagtagagat cgcttcgttg tgaacgccat
     7801 ttgaactgat aaatgctttt gtaaacttta gtcttcttaa attgtcgttg gtgatcggtc
     7861 cgacaaatgc gccggtattt ttccggaaat caccgccaat cagtagaatt tctggtccat
     7921 ctgcttgagc aagaatatca aaaactggat aattatttgt aatgatccga attttcttgt
     7981 tcagtaaatg cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa atcgtttctc
     8041 cctcgtgaat caaggaggct gctgtggcag caatttcttt tttttcttga atctgcagag
     8101 tggctttttc agtgtgagaa agttcttgat ctaaagtgta ggaaagactt tgagcccctc
     8161 catggacacg cagcagcttg cccgattttt ctaattcatc taaatctctg cgaacggtca
     8221 tatcagaaac atctaattga ttgatgattt cattaacggt aaggattcct tttttattga
     8281 ccatttctac gatagtaagc aatctttctt ttttcaacat tgtcttcacc tctaatttaa
     8341 ggataatcta tcatataaac ggacaacagt caatctaatt cgaacaaaag taaacattta
     8401 gggtggtttt tatgtttgtt acaatagatt ttattgctgt tttctggttt ttatgattat
     8461 ttgtgtttgt ttttgttgac aaatgttttg gatgcgttta caatgcaatc agttagaaag
     8521 agcgaaaact gattatagat ttgaggagag atgtacatgc ttaaattacc agaagatttt
     8581 atttttggcg gagccacagc agcctatcag gttgaaggag ccactaagga aggtggcaaa
     8641 ggcgctgttg cttgggatga ctttttagag gaacaagggc gattcagtcc cgatccggca
     8701 agtgattttt atcatcaata tgcaaaggac attgaattgt gtgaacgttt tggtgtaaat
     8761 ggtctaagat tatccattgc ttggagccgc atttttcctg atggagctgg gaaaccaaat
     8821 ccagaaggaa tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag aaacgtaacg
     8881 ccgtttgtga cgttgcatca ttttgatacc cctaaacgtt tgtttgacca tggagatttt
     8941 ttgaatcgag aaacaataga agcatttgtt tcctatgcca ttttttgttt tcatgaattc
     9001 aaagaagtca aggtgtggag tacctttaat gaaatctatc cagttgcaac caatcaatat
     9061 ttgttaggtg ttttcccacc aggaatcaaa tatgatttta caaagatcgt cgcctgtctt
     9121 cataatatga tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa cgaacttccg
     9181 ggtgaaattg gtgtagttca ctcattagag acgaaatatg cggcaacaga cgcgccggaa
     9241 gataagcatg ctgccttttt agacgatgca ttgtcgatcc gtttcttatt agatgctacc
     9301 tatttaggct attattcaac tgaaaccttg actgctcttg atgagatatg tgaagcaaat
     9361 caggccagct atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc atccacgcgt
     9421 aatgactact tagggatcaa tcactaccaa tgccattttg tcaaagctta cgatggagaa
     9481 aatgcaatcc atcataacgg tacgggtgaa aaagggacct ctgtttataa agtgaaggga
     9541 atcggagaac gtatttataa agaaggaatt cctcgaactg attgggactg gctgatttat
     9601 ccagaaggtc tatatgactt gttgctgcga attaaaagcg attacccaca ttacaacaaa
     9661 atctatatca ctgagaacgg gatggggtat aaagatcagt ttgaagatgg cattattatg
     9721 gatcagccga gaatcgacta tttgagagtc tatttggaat ccttaagcaa ggcgataaca
     9781 gccggggtaa atgtgaaggg gtattttttg tggtcgctta tggatctttt ctcatggaca
     9841 aatgggtata acaaaaggta tggcttgttt tatgttgatt ttgaaaccca gaagcgttac
     9901 ccaaaagaat cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat tatttaataa
     9961 gtgataatgg aggaagaaaa gatgaataaa gaggaattac aaatgcttgg ttttgagatc
    10021 gttgcatatt caggcgacgc tcgcagtaca ttattgaaat tattgaaaga agtaagacaa
    10081 ggaaactttg aacatgttga ttcggcgttg aaagaagccg acgaaaattt aacccttgca
    10141 cataattcgc aaacaaaaat tttagcggaa gaagcatctg ggaaagagat ggagatggga
    10201 ttcattttta tccatgggca ggatcattta atgaccactt tattgctgag agatctgatt
    10261 caagacttta ttgtactgta cagacagaat caaggatagg agaagaacgc tatggatgga
    10321 atcattaaac aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg taatagttat
    10381 ttaggagccg ttcgtgacgg atttttaaca gcgatgccag ctattttatt ttcaagcatt
    10441 ttcattttga ttgctgcggt tcctgagatt tttggttacc aatggcctga atctgtctcc
    10501 acatggctat ggaaagtcta taattattcg atgggtgttg tagggttact cgtttcagca
    10561 accactgctc gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa caaagtaatc
    10621 aacaccacct ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag cgtctctccg
    10681 attgaaggtg gattttcaac ggaatatatg gggacaaagg gactactggc ttcctttgtc
    10741 gctgcatttt taacagttaa tttatataag ttttgtgtgc tgaaagatat taccattaaa
    10801 atgcctaaag aagttcctgg aacgatttcg caaaccttcc gagatatttt tcctttctct
    10861 ttttcagtat ttgcggccgt tttaattgat ttaggcattc gacaaatctt ccaaatctca
    10921 tttgcagaag caatcattac ggtgctgcag ccgctattta cagcagctga cgggtactta
    10981 ggaatcgcta ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt tcatggaccg
    11041 tcgatcgtgg agccggcaat tgcggcaatt atctatgcca atgtggaaac caatctgcaa
    11101 ttgttcaatt ccggtgaaca agcttctaaa gtactgacgg tgggtttagg gaattttgta
    11161 ggaactatgg gaggaactgg ggcaacattg gttgttcctt ttatcttcct actctttgct
    11221 aagtctaagc agttgaaggc agttgggaaa gcttccttta ttccagtaag tttcgctgtc
    11281 aacgaaccat tgctatttgc ggcacccatg attttgaacc cttatttttt tattccattt
    11341 ttgttaacac ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt attgggcatg
    11401 aacagcttta tgtacgtttt gccttgggca actccagcac cgatcggttt ggttctagga
    11461 acgggcatgg gtatcttagc ttttgttcta gcagcggttc taatcgtcgt tgacttcctg
    11521 atctatctac cattttgcaa agcatacgat gctgtcttag tagagaaaga agcacaaaaa
    11581 gcagaggtga tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga agtacctgtg
    11641 gcagcgagtc aagttatcag cgatgcaaat aaagaaatca atgtgttggt actttgtgct
    11701 ggcgcaggga caagtgcgat gttagctaat gctttaaaaa aaggtgcatc tgaatcgaaa
    11761 gcaccgatca gtgcaatggc gggtgcttat ggttctcatt acgaaatgct gcaggatttt
    11821 gatatggtgg tcttagcacc gcaagtcaat tcttactatg atgatatcaa agaagacacc
    11881 gatcgcttag gaatcaagct ggttgcgaca aaaggagcag aatatatcgg attaacgaga
    11941 gatcctgaaa aagctgttca gtttattctg aaccaatttc aataaaggag gagccataat
    12001 gaaaatggga atttggttgc gtaaagctgt tttatttgca gctgtgttag gggctttggg
    12061 ggctgtttca aaaactgttg aagcatctgc cagcgaattt attcgcggag cagatatttc
    12121 gatcctgtca gatatggaaa aatctggtgc aaaatattat gaagacgata tttcaaaaga
    12181 tgccttagag attttaaaaa ataatggtgt caactatgtt cgactgcgtt tgtggcagga
    12241 tccttataat agtaatggag aaagttatgg agcagggaca aatgatctgg aaacgacgat
    12301 tgaccttgca aaacgagcaa aaaatctggg acttaaggtg ctgctggatt ttcactacag
    12361 tgatttttgg gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag gtctgacctt
    12421 tgaagaaatg aatacggcat tgtacgacta tacaaagaac gttctgtcgg aaatgaagca
    12481 gctggacgtt tatcctgata tggttcagat cggcaatgaa ctgaatagcg gaatgctctg
    12541 gccttatggc aagtcttggg gagaaggcgg gggagaattt gatcgattgg ctgctttttt
    12601 gaagtctggt atccaagctg ttcgagacac tcaacctaaa acaactccag taatgctcca
    12661 cttagctgat ggtggcgata caggagcatt tacttggtgg tttgatgaaa tcacgagtcg
    12721 aggtgtgtca tttgatttaa ttggagtgtc gtattatccg tattggcacg gttcaatgaa
    12781 tgaacttcaa accaatatga atgcgatcag ccaacggtat ggtaaagaag tgatcgtagt
    12841 tgaaacagct tatgggcata caacagcgaa tgccgatact atgccaaatg cttttggtga
    12901 agccgaagca gctgcggggg gatatgaacc aacaccgaca ggacaagcag agtatttgct
    12961 ggatcttgcc gatcggattc aggcagtacc gaataatcgc ggggcgggct tcttctattg
    13021 ggaacctctt tggtacaacg gaaatgtgag ttgggcaaca caagcaggta tgaattatct
    13081 cagtgttcaa tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg attttgctgg
    13141 aaacgccttg ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa atctagttaa
    13201 aaatgcaagt ttcgaaagcg atggaccaac gaccagtcct tccaattggc aaacctggtt
    13261 tcaacaagga accgctaagg gtacgatcaa aacggaagcg ggcacttttt ttggggacta
    13321 caaactaact ttttggcagg atcaagcata cgaggcatca gcttatcaaa atgttagtgg
    13381 cataagcaaa ggaacttaca cattgagtgc ctatgtaatg tctgggggag agtttgatac
    13441 taatcagtta tatatcaaaa attatggtgg agcggaacta aatactgcgg ttgttaaaac
    13501 aagtgaatgg acaaaaatta caatcaaaga cattccaatt acaacaggtg tttgtgaaat
    13561 tggggtttat agcaaggctt caggaaatgc ttggacgaac gttgattttg tttcattggt
    13621 gaaacaatag actgcggatc gtttcgattc aagatcggag gttcccttca gccacatcgt
    13681 tagcgtagaa ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa ccagaaacga
    13741 tttccagtca tttttaccaa aacgagtaaa gcaccagact tcatctacac tttgtattat
    13801 gctaaagaga gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa agttgtgcag
    13861 ataacataaa gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa tatgatttga
    13921 gcacttagat ggatagaaaa aagttctcgg aaaacagttc gtttttcgag aacttctttt
    13981 tatgataaga atgaaacaaa ttaacttata ttatagtctc ttttggtagg gaaccatctt
    14041 atttttaatg aaaatcaagc attttagcta ttgaagaatg ttgatttata gggattcccc
    14101 ctccatatta tgttggctct ttattcccac tccactgttg ctggtggttt agaagtgatg
    14161 tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga gattttttgc
    14221 aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga agttacagca
    14281 cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac agagcggatc
    14341 cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc tgcgatttct
    14401 tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt gatttcgcca
    14461 agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat tgaatcaggc
    14521 atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt taatggttcg
    14581 atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga tttgatcgtc
    14641 tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg tgctaggaaa
    14701 gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata tacgaattca
    14761 ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt gtctaagaag
    14821 cgatctcttg cattgacttt gatgatgttc aagccgaatt ttccgcctag actttccatt
    14881 acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg
    14941 tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc acctgaaaga
    15001 ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg catatcgata
    15061 aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa gtggcgcagc
    15121 aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc ataaaaatta
    15181 cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac tgtttcaaag
    15241 ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg ttgttttggt
    15301 gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt cccatattcg
    15361 cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg cataccatag
    15421 caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc aaatgcattc
    15481 tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt catcgctttt
    15541 acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag atcacgaata
    15601 cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat cttttcaacg
    15661 ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa ttttatctca
    15721 aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat cgatgatatg
    15781 attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga tatattcctg
    15841 taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg cttcttcacg
    15901 atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg tatccagtaa
    15961 cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat cgacatagac
    16021 agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt
    16081 gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt ctggaatgat
    16141 atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag attttacacg
    16201 gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt tacggtccat
    16261 gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg
    16321 aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg ctgttgtact
    16381 ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt ctacgtattt
    16441 atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat aaagataaat
    16501 cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc ggtcattcaa
    16561 acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac gataaaattc
    16621 tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat tagcctcctg
    16681 cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca ccacttaaga
    16741 gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc ttattcttga
    16801 cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag aaggatggaa
    16861 gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg
    16921 gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt attttcacgt
    16981 gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga agagttacct
    17041 gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt agctttggca
    17101 tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt aagtctagcg
    17161 caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc gaatatctat
    17221 gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat ccgtgctgga
    17281 aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa gggcggtaca
    17341 ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa
    17401 acatttggta atgcagaaat cgtcacaaaa gataaaggat attatatcat caaaagtgtg
    17461 aaagaataac tagctgaaaa atagtggagc gacgaaagag tggaactggg cgtgcatgta
    17521 cgggtacgta tcagttcttt ttttgttgag aagtttttaa aaacaaagaa ctattttttc
    17581 ttcaaaaaga aatagtagga acagagtcgt tgttttttac gataaaataa ataagataca
    17641 gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac
    17701 tttccttttt tctctattct tttcttgggg gttttggtac actgataggg aatttagata
    17761 gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc
    17821 agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag
    17881 aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa
    17941 tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt
    18001 cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat
    18061 gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca
    18121 ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt
    18181 agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg
    18241 ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt
    18301 tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt
    18361 agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg
    18421 gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt
    18481 actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg
    18541 ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt
    18601 cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag
    18661 ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag
    18721 aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt
    18781 gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg
    18841 ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt
    18901 ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa
    18961 aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg
    19021 aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac
    19081 atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc
    19141 gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac
    19201 acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg
    19261 tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga
    19321 tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg
    19381 gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac
    19441 gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc
    19501 gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag
    19561 aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac
    19621 cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat
    19681 agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta
    19741 gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc
    19801 gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc
    19861 aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt
    19921 ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc
    19981 gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa
    20041 gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca
    20101 aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa
    20161 ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac
    20221 atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa
    20281 atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct
    20341 gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca
    20401 gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta
    20461 atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc
    20521 atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag
    20581 gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg
    20641 cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat ccgagcttgc
    20701 gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc
    20761 ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc
    20821 aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct
    20881 acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat
    20941 taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta
    21001 ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg
    21061 taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa
    21121 cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta
    21181 cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac
    21241 aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg
    21301 tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat
    21361 aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt
    21421 tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt
    21481 atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc
    21541 agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa
    21601 ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa
    21661 tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca
    21721 agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat
    21781 ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga
    21841 tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg
    21901 tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg
    21961 tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga
    22021 attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa
    22081 aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc
    22141 aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa
    22201 aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa
    22261 cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga
    22321 agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg
    22381 attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc
    22441 agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga
    22501 agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg
    22561 attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca
    22621 actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca
    22681 atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg
    22741 tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca
    22801 atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat
    22861 ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc
    22921 gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg
    22981 tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa
    23041 gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag
    23101 tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg
    23161 gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt
    23221 ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga
    23281 aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca
    23341 aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg
    23401 ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca
    23461 aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg
    23521 tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg
    23581 aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga
    23641 ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata
    23701 aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat
    23761 acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa
    23821 aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat
    23881 tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag
    23941 gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga
    24001 ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc
    24061 tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa
    24121 ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg
    24181 atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag
    24241 aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc
    24301 cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg
    24361 atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt
    24421 ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca
    24481 ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca
    24541 ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt
    24601 gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt
    24661 ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac
    24721 tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc
    24781 tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta
    24841 acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt
    24901 attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga
    24961 ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg
    25021 atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat
    25081 caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc
    25141 ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg
    25201 aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta
    25261 tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct
    25321 aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg
    25381 aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc
    25441 ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt
    25501 ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta
    25561 gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg
    25621 ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag
    25681 tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt
    25741 taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga
    25801 atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct
    25861 ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga
    25921 tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg
    25981 ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct
    26041 tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg
    26101 gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc
    26161 ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg
    26221 gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt
    26281 tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg
    26341 ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa
    26401 gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat
    26461 aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc
    26521 agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc
    26581 aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg
    26641 tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg
    26701 gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg
    26761 tgtgtttcct aatggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg
    26821 taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca
    26881 ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc
    26941 ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga
    27001 tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga
    27061 accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca
    27121 cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa
    27181 cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg
    27241 cgttgaccag ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt
    27301 agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat
    27361 tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct
    27421 aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga
    27481 atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg
    27541 atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa
    27601 tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc
    27661 aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa
    27721 attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat
    27781 gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat
    27841 gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga
    27901 ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc
    27961 ggactagggg cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt
    28021 gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg
    28081 atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg
    28141 cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa
    28201 ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct
    28261 cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct
    28321 cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa
    28381 gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa
    28441 gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca
    28501 tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg
    28561 agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg
    28621 tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat
    28681 gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa
    28741 attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt
    28801 actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca
    28861 ttttcctcca atcagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa
    28921 gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt
    28981 gacagagacg ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc
    29041 tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga
    29101 ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat
    29161 gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct
    29221 gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga
    29281 cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg aatctgccgt
    29341 ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg
    29401 tgacttgtga ccgagaagtt ggcaggagaa aaccgtttat acgaaaaaag ggactgtgac
    29461 aaatcgtgtc acagtccctt ttttatagtg ttttttatag gtagtatttt ttagcaactg
    29521 ttaagtcatc atttaattca taaacaagtg gttgacctgt tggaatttca agatccatga
    29581 tgtcttcgtc agagatacct tcgatatgtt tagctagggc gcgtaatgag ttaccatgtg
    29641 cagctactaa aacagttttg ttgtctagta atgctggagc aatttcatct tgccagaatg
    29701 gcaaagcgcg ttctaatgtc actttcaagt tttctccacc aggaatatcg cgtttgtcta
    29761 acattgcgta acgacggtca tttgctgcag aaccttcgtc agtttcttcc attaatggag
    29821 gaagagtatc gtaagaacga cgccagatgt gcacttgttc gtcgccgtat ttttcagctg
    29881 tttctttttt gttcaaacct tgtaatttac catagtgacg ttcgtttaag cgccatgatt
    29941 tgatttgtgg tacccaaagt tgatctgaat attctaagat caagttacat gttttgattg
    30001 cacgagttaa aacagaagtg taagcaacat caaattcgat tccagcttct ttgattttgc
    30061 gtccgccttc ttttgcttct tcgattcctt ctggtgctag atttacatca gcccaaccag
    30121 taaattggtt caatgcattc cattcactaa gaccgtgacg agaaaaaact aattttggca
    30181 tgtttagctc tccttttaag ataggtttat atttctgaaa ttttcagttc tgttttattg
    30241 tacaattttt tggaagagat ttccatcgaa aaattagata agattctatg agaaaacaag
    30301 tagagaatat aaaaaaacga acagtgccca cgcaactgtt cgtttttagg tattagatgc
    30361 ataattgctc gatttctttt tcaggtaaat tgatatattt ataacatgtt ccaactgaaa
    30421 caccgcattt tgaagaaata aattggatcg tttctttttt ttcataatag agacgtcgga
    30481 tttttttgat cgtttttgca tcgatttttg gtcttccgcc gatttttcct tttttacgag
    30541 cttcatctaa tccaactaat gtacgttctt tgatcaatgc gcactccatg tttgctaggt
    30601 tttccattaa ttggaaatag atttttccca tttcgcgcgt atcgattcct tcatcaagac
    30661 taatcaagtg cagcccgcga tctttgaata attgcgtgag ttctgttaat tgccgcgtag
    30721 atttgcctaa tcgatgaagt tggcaaatga ctagtgcatc gccttgttgc atgtcagcga
    30781 tcactgactc caaatgaaca gcagtagaat cattttgttc cacttcatat gtctcatgga
    30841 aaatttcttg acaaccaaag tcagataatt gatcaacctg agttgttaaa tcaacgtcat
    30901 ttatggttgt gcgtgcataa ccaataattt tcatggtgat tcctttctag cagtgatttt
    30961 ctaactggat ataacagtaa aaatgtagca taaagtacgt caaaaagata gttttttcgc
    31021 aaattttccg ctgtcttttt ttattggatg atcaagcagt ttttctttat ttacgacgga
    31081 ttgcgtcaag aatctcaacg cctttttctg agccgacaac tactttagaa accatgttta
    31141 ggaaaagccc atgttctact accccaacaa gttcatctaa ataggtagct aagctttctg
    31201 gctgttcgat gacttctaga tgcagatcaa tgatgtaatg accaccatct gtggttaaga
    31261 cttctccgtt ttcgtctgta cgcaagacgg gttggaatct tttttcttca aaaatatgaa
    31321 gtagctgttg ggagccataa gggatcactt caactgggag tgggaacttc ccaagtttat
    31381 cgactaattt agaagaatca acgatccaaa tcgtttcttt tgagtacgta gcgactattt
    31441 tctcgaaaag caaagcagcg ccgccgcctt tgatcccttg aaaatcagca ctgatttcat
    31501 cagcaccgtc tattgtcaaa tcgactacgg ggacttcatc aatactcttt aacggtattc
    31561 ctaatttttc cgcttgttct tttgtcgcat ttgaagtagt aactcctgtg atacgtaaac
    31621 cttcttcacg catacggcga ccaatctctt ctaccatata ataggcggtt gaaccggtcc
    31681 cgagacctac gatcatcccg tcctttacga atttcgcggc ttcaatgcca gctaattgtt
    31741 ttaaattcat ctttgcgtcc accttttttt agtttgagca tcgtgctttc ctcactattt
    31801 tagcgtgatt tacggtaaaa agataccatt cttttttatt ccaataaagc tttttcaaaa
    31861 aaaattgcat ctcttgttga catgtttttt cattcgtgat attctaacaa aggtgcttta
    31921 tctacaggtt tctgtgaaga aacgtgctga ctgtttatca aagcatattt gataaggatg
    31981 cagacattgc atttattttt tatcacaaaa aaagaaccac ctggatgtgt ggaactagac
    32041 gcaaatcgtg ggaaggagga aacaaggaat gcctacaatt aaccaattag tacgtaaacc
    32101 tcgtaaatca aaggtggaaa aatctaattc acctgctttg aacaaaggat ataacagctt
    32161 taagaaaact caaactaacg taaactcacc tcaaaaacgt ggggtagcaa ctcgtgtggg
    32221 tacaatgaca cctaaaaaac cgaactcagc tttacgtaaa tatgcacgtg ttcgtttgtc
    32281 aaacttaatt gaagttactg cttatatccc agggattggt cataacttac aagaacacag
    32341 tgttgtatta ttacgcggtg gtcgtgtaaa agacttacca ggggtacgtt accatatcgt
    32401 tcgcggtgct ttagatactg ctggtgttaa cgaccgtaaa caaagccgtt ctaaatacgg
    32461 aactaaaaaa cctaaagctt aattttaatt attaaacaaa tgaaacttgc ttctactata
    32521 tagaagtaat tactatgaac aatatttcgg aaggaggagt tacggatgcc acgtaaaggt
    32581 cctgttgcaa aacgtgatgt tttaccagat cctatttata actcaaaatt agtaactcgc
    32641 ttgatcaacc gtgtaatggt tgacggaaaa cgcgggattg cagctaacat tatctataat
    32701 gcctttgact tgatcaaaga atctacaggt aacgatccat tagaagtgtt tgaacaagca
    32761 atgaaaaacg ttcaacctgt tcttgaagtt aaagcacgtc gtgttggggg ttcaaactac
    32821 caagtaccag ttgaagttcg ccctgaacgc cgcgttactc taggtcttcg ttgggtagta
    32881 aactacgctc gcttgcgcgg tgaacacaca atggaagaac gcttagcaaa agaaatcatg
    32941 gatgctgcaa acaacacagg tgcttctgtt aaaaaacgtg aagacactca taaaatggct
    33001 gatgccaacc gtgcgtttgc tcactatcgc tggtaagatt ctctctgttt ggtcggcttt
    33061 tataagtcga cctcagatag tgtccttatt tttagcaact agatagtaaa gagaggagaa
    33121 acaaggagaa tgaccagaga attttcttta caaaacactc gtaatatcgg tatcatggcc
    33181 cacatcgatg ctggtaaaac gactgctact gaacgtatct tgtactatac tggtaaaatc
    33241 cataaaattg gtgaaactca tgaaggagct tcacaaatgg actggatgga acaagaacaa
    33301 gaacgtggta ttacgatcac ttctgctgct actacagcac aatggaaggg tcaccgaatc
    33361 aacatcatcg acacaccagg acacgtagac ttcacagttg aagttgaacg ttcattgcgt
    33421 gtacttgatg gtgctgtaac tgttcttgat gcacaatcag gtgttgaacc tcaaactgaa
    33481 acagtttggc gtcaagcaac aacttatggt gtacctcgta tcgtatttgc aaacaaaatg
    33541 gacaagatcg gtgctgactt cttatattca gtatcaactt tacatgaccg tttgcaagca
    33601 aacgctcacc caatccaatt accaattggt gcggaagacg acttcacagg aattatcgac
    33661 cttgtaacaa tgaaggctga aatgtataca aacgatcttg gaacagagat cgaagaaaca
    33721 gaaattcctg aagaataccg cgaattagct gaagaatggc gcgaaaaatt ggttgaagct
    33781 gtagctgaaa ctgatgaaga acttactttg aaatacttag aaggtgaaga aatcactgaa
    33841 gctgagttaa aagaaggtat ccgccgcgca acagttaacg tagaattcta cccagtacta
    33901 tgtggttcag catttaagaa caaaggggtt caattactat tggacgctgt tcttgattac
    33961 ttgccatcac cgttagatat cccagctatc aaagggattg accctaaaac tgatgaagaa
    34021 gtagaacgtc ctgcagacga cagcgctcct ttctcagctt tagcatttaa agttatgact
    34081 gacccattcg taggtcgttt aactttcttc cgtgtgtact ctggtgtttt acaatcaggt
    34141 tcatacgtac aaaacgcaac aaaaggcaaa cgtgaacgtg ttggtcgtat cctacagatg
    34201 catgccaact ctcgttcaga aatctctgaa gtttacgctg gagatatcgc tgctgctgtt
    34261 ggtttgaaag acacaactac tggggacaca ctatgtgatg aaaagagtct agttattctt
    34321 gaatccatgg aattcccaga accagttatc caagtcgcta ttgaacctaa atcaaaagct
    34381 gaccaagata aaatgggtgt ggctttacaa aaactatctg aagaagatcc tactttccgt
    34441 gctgaaacaa acgtggaaac aggcgaaaca atcatcgctg gtatgggtga acttcactta
    34501 gatatcatcg ttgaccgtat gcgtcgtgaa ttcaaagttg aagctaacgt aggtgcgcct
    34561 caagtatctt accgtgaaac tttccgtgct ggcactcaag ccgaaggtaa gtttgtacgt
    34621 cagtctggtg gtaaaggtca atacggtcac gtatggattg aatttacacc aaatgaagaa
    34681 ggtgctggtt tcgaatttga aaatgcgatc gtcggtgggg ttgttcctcg tgaatacatt
    34741 ccagctgttg aaactggttt gaaagatgct atggaaaatg gtgtcttagc tggctatcca
    34801 ttagtagata tcaaagctaa actttacgat ggttcatacc atgatgtcga ttcaaatgaa
    34861 actgccttcc gtgttgctgc ttctatggca ttacgtgcag cagctaagaa agctaatcca
    34921 gtaatccttg aaccaatcat ggctgtagaa gttgttatcc ctgaagatta tttaggtgat
    34981 gttatgggtc acgtaactgc tcgtcgtgga cgtgttgaag gtatggaagc tcgcgctggc
    35041 ggccaacaag ttgttcgtgc gatggttcca cttgctgaaa tgtttggtta tgctacaact
    35101 cttcgttctg ctactcaagg tcgtggtaca tttacaatga catttgacca ctacgaagat
    35161 gtaccaaaat cagtacaaga agaaatcatt aagaaaaacg gcggaaaagc tgaataattt
    35221 cttaaaaccg gatagtcctg caaagagatt atccaaagag actatatttt taatatagtt
    35281 tcgtgtaaaa taattatcga tgatatgggc atgtggtgta ccttgtgtac atcatatacc
    35341 tatcagacaa ataaaaaata tattgtattt ttaggaggac attttaaaat ggcaaaagaa
    35401 aaatttgacc gttctaaacc acacgttaac attggtacta tcggacacgt tgaccatggt
    35461 aaaactacat taacagctgc aatcacaact gtactatcta agaaaaacgg cggccaagct
    35521 atggcttacg atcaaatcga tggtgctcca gaagaacgcg aacgcggaat cacaatctca
    35581 acagctcacg ttgagtatga aacagataca cgccactacg ctcacgttga ctgcccagga
    35641 cacgcggatt acgttaaaaa catgatcact ggtgctgctc aaatggacgg agctatcttg
    35701 gtagtttctg ctgctgacgg cccaatgcct caaactcgtg aacacatcct attgtctcgt
    35761 caagttggtg ttccttacat cgttgtattc ttgaacaaag tagacatggt tgatgacgaa
    35821 gaattactag aattagttga aatggaagtt cgtgacctat taacagaata cgaattccct
    35881 ggtgacgatg ttcctgtagt tgctggatca gctttgaaag ctctagaagg cgacgcttca
    35941 tacgaagaaa aaattcttga attaatggct gcagttgacg aatacatccc aactccagaa
    36001 cgtgacaacg acaaaccatt catgatgcca gttgaagacg tgttctcaat tactggacgt
    36061 ggtactgttg ctacaggtcg tgttgaacgt ggacaagttc gcgttggtga cgaagttgaa
    36121 gttgttggta ttgctgaaga aacttcaaaa acaacagtta ctggtgttga aatgttccgt
    36181 aaattgttag actacgctga agctggagac aacattggtg ctttactacg tggtgttgca
    36241 cgtgaagaca tccaacgtgg acaagtttta gctaaaccag gtacaatcac acctcataca
    36301 aaattctctg cagaagtata cgtgttgaca aaagaagaag gtggacgtca tactccattc
    36361 ttcactaact accgtccaca attctacttc cgtacaactg acgtaacagg tgttgttgaa
    36421 ttaccagaag gaactgaaat ggttatgcct ggcgacaacg taacaatgga agttgaatta
    36481 atccacccaa tcgctatcga aaacggtact aaattctcaa tccgtgaagg cggacgtaca
    36541 gttggtgccg gtgtcgttac agaaatcaaa gcttaattca cttaagtttt aaagtaaagc
    36601 aacatttatt cggacgtaat cgctcggaac gaaggtctta ggatctttgt tccgagcttt
    36661 tttatttatt gcaggatcag gaaaaaagac aggagtatta taaatagaag aaacacgaaa
    36721 taaaagagtc agtattttct aaatgaaact ttatgaaaac aaaatgtttt ttacaagaaa
    36781 aagccttgct ttgtgagttt atatatatta taatgacaaa ggtgcgttta ctttagtgaa
    36841 ttgcacagac gtaatcagaa tgatttcgtc ggctaagaaa cgagaggttg cgacacgccc
    36901 ggacgctttg ccacgaacga gcgtgacgga aattttcgtg gagctatgtc tacttttcaa
    36961 aataggcgaa ggagggaaca agatggcaaa acaaaaaatt cgtatccgtt taaaagcgta
    37021 tgaacaccgt attttagatc aatcagcgga taaaatcgtg gaaactgcaa aaagaactgg
    37081 agctagcgtg tctggtccaa ttccattacc aactgaacgc agtctttaca caatcatccg
    37141 tgcgactcat aaatacaaag attcacgcga acaattcgaa atgcgtacac acaaacgtct
    37201 tatcgacatt gtgaacccaa caccaaaaac tgttgatgct ttgatgaagc tagacttacc
    37261 atctggtgta aacatcgaaa tcaaattata aaactaaaac aaaaataatg gaggtgtact
    37321 catgaccaaa ggaatcttag ggaaaaaagt gggaatgaca caaatcttca ctgaatctgg
    37381 tgaattaatt ccagtaactg ttgtagaagc tactccaaac gtagtactac aagtgaaaac
    37441 aatggaaacg gatggctacg aagctattca agttggttac caagatatgc gtgaagtttt
    37501 atcaaacaaa cctgcgaaag gtcatgttgc aaaagcaaac acggctccta agcgcttcat
    37561 tcgtgaattc aaaaatgttg agctaggaga atatgaagta ggaaaagaaa ttacagtaga
    37621 tgttttccaa gcaggagaca ttattgatgt aacaggtact acgaaaggta aaggattcca
    37681 aggggttatc aaacgccacg gacaaagccg cggaccaatg gctcacggtt ctcgttacca
    37741 tcgtcgtcct gggtcaatgg gtccagttgc acctaaccgt gtatttaaaa acaaacgtct
    37801 tgctggccgt atgggtggca accgcgtaac tatccaaaac ttggaagttg tacgtgtcga
    37861 tgcagaaaga aatgttatct tgatcaaagg taatgttcct ggagctaaaa aatctttaat
    37921 tactatcaaa tcagctgtga aagctaaata attgtaggaa gaaaggagga actaaggaat
    37981 gccgaatgta gcattattca aacaagatgg aagccaaaac ggtgaaatca cacttaacga
    38041 agaaatcttc ggaattgaac ctaatgaaag tgttgtttac gatgcaatcg taatgcaacg
    38101 tgcgtcatta agacaaggaa cacacgcggt taaaaaccgt agcgctgttc gcggcggcgg
    38161 acgtaaacca tggcgtcaaa aaggaacagg tcgtgcacgt caaggatcta tccgctcacc
    38221 acaatggcgt ggaggtggcg tagtctttgg accaacacca cgttcttaca gctacaaact
    38281 tcctaaaaaa gttcgtcgtt tagcaatgaa atctgtattg tcagaaaaag ttgctgaaaa
    38341 caacttggta gcaatcgaag gcttgaactt cgacgcacca aaaacaaaag aattcaaaca
    38401 agttcttgct aacttatcta tcgactctaa agtattggtt gttctagaaa aaggtaacga
    38461 ctttgctgct ttatcagcac gtaacttacc aaacgtttct gtagtaactt ctgataatgt
    38521 gagtgtgcta gacgttgtat caaatacaaa agtgttagca acacaaactg ctcttactca
    38581 aattgaggag gtgcttgcat aatgaactta ctagacgtaa tcaaacgccc agtgatcact
    38641 gaaaaatcaa tgcttgccat ggatgacaag aaatacactt ttgaagtgga cactcgcgca
    38701 aacaaaactt tagtaaaaca agcggtagaa gctgcttttg acgttaaagt caaaaacgtg
    38761 aacatcgtga acgtacgtgc gaaattcaaa cgtatgggca aatatgcagg atatacaaaa
    38821 aaacgtcgca aagcgatcgt tacattgact gaagattcaa aagaaatcca attattcgaa
    38881 gctgctgaat aagcactaat agataactca ttaaattagg agggaaaaac gtggcgatta
    38941 aaaagtacaa acctacctca aatggtcgtc gtaacatgac aggttctgat tttgctgaaa
    39001 tcacaacatc aacacctgaa aaatcattgt tacaaccatt gaaaaaccat gctggacgta
    39061 acaacaacgg ccgcatcact gtacgtcatc aaggtggcgg acacaaacgc caatatcgcg
    39121 tgatcgactt caaacgtaac aaagataacg ttgttgcaac tgttcaaacg atcgaatatg
    39181 atccaaaccg ttcagctaac atcgcgttag tacactatga agatggagtg aaagcttaca
    39241 tcttagcacc taaaggacta gaagtgggta tgaaacttgt ttcaggtcca gaagcagata
    39301 tcaaagtcgg aaatgctctt ccattagaaa acattccagt tggtactgtt atccacaaca
    39361 ttgaaatgaa acctggtaaa ggtggacaat tgatccgttc tgctggtaca agtgctcaag
    39421 tacttggtaa agaaggcaaa tacgtattga tccgcttgaa ctctggtgaa gttcgtatga
    39481 tcttagctgc atgccgtgct acaatcggtt ctgtaggtaa cgaacaacac gaattaatca
    39541 acatcggtaa agctggacgc tctcgttgga tgcgtaaacg cccaactgta cgtggtagcg
    39601 taatgaaccc taatgatcac ccacacggtg gtggtgaagg taaagctcct atcggacgta
    39661 aagcaccagt ttctccttgg ggtcaaccag ctcttggtta caaaactcgt aacaaaaaag
    39721 ctaaatcaga caaacttatt gttcgtcgtc gcaaaaataa ataattactt tgaccatgtg
    39781 tcgcacattt tgagaggagg ttcaccatgg gtcgtagttt aaagaaagga ccttttgtcg
    39841 atgagcattt aatgaaaaaa gttgaagctc aagcaggggt tgaaaagaaa aaagttatca
    39901 aaacttggtc acgccgttca acagttttcc caagttttgt tggatatacc atcgcagttt
    39961 atgatggacg taaacatgta ccagtttaca tccaagaaga catggtagga cacaaattag
    40021 gtgaatttgc acctacaaga acttatcgtg gacatgctgc tgacgataag aaaacgaaac
    40081 gctaatttga ggggaggata aatccaaatg gcagaacaaa tcacatcagc taaagcaact
    40141 gcaaaaacag ttcgcacttc acctcgaaaa gcccgtttag taattgacct tatcagaggt
    40201 aaaagtgtcg cagatgcaat ttcaatcttg aaattcacac caaacaaatc tgctggaatc
    40261 attgaaaaag tattgatgtc agcagttgcg aatgcagaaa ataactttga cttagatgtt
    40321 gaaaacttgg tagtaagcga agcttttgtt aacgaaggac ctacaatgaa acgtttccgt
    40381 cctcgtgcaa aaggatcagc ttctccaatc aacaaacgta caagtcatat cacggtagtc
    40441 gtatcagaaa aataaggagg gataatcagt gggtcaaaaa gtacatccaa ttggaatgcg
    40501 tgtaggcatc atccgcgact gggatgctaa atggtatgct gaaaaagagt atgctgagtt
    40561 cttacacgaa gatttaagaa tccgtaaatt tatttcaact aaacttgctg atgctgctgt
    40621 atcaacagtt gaaatcgaac gcgcaacagg tcgcgtaaac atttcaattc acacagctaa
    40681 accaggtatg gttattggta aaggcggttc tgaagtcgaa agcctaagaa aagaattaaa
    40741 caaattaact ggtaaaagaa tccatatcaa tatcgtggaa atcaaaaaac cagatctaga
    40801 tgcaaaatta gtaggtgaag gaatcgcacg tcaattagaa aaccgtgttg ctttccgtcg
    40861 cgctcaaaaa caagccatcc aacgctcaat gcgtgctggc gctcaaggga tcaaaactca
    40921 agtatctggt cgtctaaacg gagcagatat cgctcgttca gaaggatact cagaaggaac
    40981 tgttcctctt catactttac gtgcggacat tgattacgca tgggaagaag cagatacaac
    41041 atacggaaaa ctaggagtta aagtgtggat ctatcgtgga gaaattcttc caactaaaaa
    41101 aaacactgag aaaggaggga aataatcatg ttagtaccta aacgtgtaaa acaccgtcgt
    41161 gaattccgcg gaaaaatgcg cggggaagct aaaggcggaa aagaagtagc attcggtgaa
    41221 tacggtttgc aagctgttga ttcacattgg atcacaaacc gccaaatcga agctgctcgt
    41281 atcgcaatga ctcgttacat gaaacgtggt gggaaagtat ggattaaaat tttccctcac
    41341 aaatcttata ctgccaaagc aattggggta cgtatgggtt ctggtaaagg ggcacctgaa
    41401 ggatgggttg caccagtaaa acgtggtaaa atcatgtttg aaatcgcagg cgttcctgaa
    41461 gaagtagctc gtgaagcgtt acgtctagct tctcacaaat taccaatgaa aactaagatc
    41521 gtaaaacgtg aggaaatggg tggtgaatcg aatgaaggtt aaagaaatca gagaattaac
    41581 cactgccgaa atgcttgatc aagaaaaaca attaaaagaa gaattgttta atcttagatt
    41641 ccaattagca acaggtcaat tagaaaacac tgcacgtatt aaagaagtac gtaaatcgat
    41701 tgcacgcatc aaaactgtgt tgcgtgaaca agctaagtaa ttatggaagg aggccattaa
    41761 gcgtatgact gaaggaagaa atcaacgcaa agtttatcaa ggtcgcgtgg tttcagacaa
    41821 aatggataaa acaattaccg ttgctgtcga aacaaagaaa aaccacccta tctatggcaa
    41881 acgcatgaac tattctaaga aatacaaagt gcatgatgaa aacaacacag caaaagttgg
    41941 cgacatcgtg agaattatgg aaactcgtcc attatcagct acaaaacgtt tccgtttgtt
    42001 ggaagtagtc gaagaagcag ttattatcta ataacacgag attttcctat atagattgaa
    42061 tgcaaaatct gaaaggagga tacacaacgt gatccaagca gaaagtcgtt taaaagtcgc
    42121 tgataactca ggcgcacgtg aaattttgac gatcaaagtc cttggtggat ctggtcgtaa
    42181 aactgctaac atcggtgatg tgatcgttgc tacggttaaa caagcaacgc caggtggggt
    42241 tgtcaaaaaa ggtgacgtcg ttaaagccgt tatcgttcga acaaaatcag gagctcgtcg
    42301 tacagacggt tcatatatta aatttgatga aaatgctgca gtgattattc gtgatgataa
    42361 aagccctcgt ggaacacgta tctttgggcc agttgcacgt gaactacgtg aaaacaactt
    42421 catgaagatc gtttcactag caccagaagt tttataatct tgagacactt atcaaaggag
    42481 gtgcgaaaca gttatgtttg ttaaaaaagg cgataaagtt aaagttatca ctggaaaaga
    42541 caaaaacaaa gaaggcgttg tactagcagc gttcccgaaa aaagataaag ttattgtcga
    42601 aggtgtaaat gtcgttaaaa aacaccaaaa accttctcaa gctgctcctc aaggtggaat
    42661 tgttgagatg gaagcgccaa ttcatgtttc taacgtgatg gtggttgact ctactggtgt
    42721 agctggccga gttggctaca aagaagtaga cggcaaaaaa gtccgtgttt ctaaaaaaac
    42781 cggtgaagtt ttagataaat aaaagtttaa ggaaggaggg tcttactgaa tgaaccgcct
    42841 aaaagaaaaa tatcttaaag aagtaactcc atcattgatg gaaaaattta actatagctc
    42901 tgttatgcaa acacctaaag ttgaaaaaat cgtcatcaac atgggtgttg gtgatgcggt
    42961 atcaaacgct aaaaacttag ataaagcagt tgaagaatta actttgatca ctggtcaaaa
    43021 accaatgatt acaaaagcta aaaaatcaat cgctggattc cgtttacgtg aaggaatgcc
    43081 aattggtgca aaagttacat tgcgcggaga aagaatgtac gaattcttag acaaattggt
    43141 ttctgtttca ctaccacgtg tacgtgactt ccatggtgta agtaaaaaag cctttgacgg
    43201 tcgtggaaac tatactttag gtattaaaga acaattgatc ttcccagaag tagattatga
    43261 tttagtagat aaagtacgtg gtatggacat cgttattgta acaacagcaa actctgatga
    43321 agaatctcgt gagttgttga cacaattagg catgccattc caaaaataat taaaggaggc
    43381 gaactacgtg gctaaaaaat caatgattgc taaaaacaaa cgtcctgcaa aattttcaac
    43441 acaagcttat actcgttgcg aacgttgcgg acgtccacat tcagtttatc gtaagttcca
    43501 tctttgccgt atttgcttcc gcgaacttgc ctataaaggt caaattcccg gcgtgaagaa
    43561 agctagctgg taaaaagtaa attcgcataa gaaggaggta aaagatcaat ggtcatgaca
    43621 gatccaattg cagattttct aactcgtatc cgtaacgcaa acatggttaa acatgagtct
    43681 ttagaagtac ctgcatcaaa aattaaacgt gacatcgctg aaatcttgaa acgtgaaggt
    43741 ttcgttcgtg atgttgaata catcgaagat gacaaacaag gcgtgatccg tgttttccta
    43801 aaatttggta aaaacggcga acgtgttatc acaaacttaa aacgtatctc taaaccaggt
    43861 ttgcgtgctt atgtcaaagc tgacgaagta ccaaaagtat tgaacggttt aggtatcgca
    43921 atcatttcga cttctgaagg tgttgtcact gataaagaag ctcgtgaaaa aaatatcggc
    43981 ggcgaagtta tcgcttacgt atggtaatca agtagaatct acaaggaggt gtctttaagt
    44041 gagccgtatc ggaaataaag tcgttgttat ccctgaagga gtaacagtta ctcaagacgg
    44101 aaacaacgtt acagttaaag gaccaaaagg tgaattgact cgtacttttt cagcagatat
    44161 caaaatgaat atcgaaggaa acgaagtaac tttcactcgt ccaaacgaca gtaaagaaat
    44221 gaaaacaatt catggaacaa ctcgtgcgaa cttcaacaac atggttgtag gtgtcagcga
    44281 aggtttccaa aaagcgttag aactaatcgg ggttgggtac cgtgcacaat tacaaggatc
    44341 aaaacttgta ttgaacgttg gttactcaca tccagtagaa atcacaccac cagctggagt
    44401 aactgttgaa gtaccagcta acacacaagt aattgttaaa ggtgcgaaca aagaagaagt
    44461 tggcgaatta gctgcaaaca ttcgtggaac tcgtcctcca gaaccttata aaggtaaagg
    44521 tattcgttat gttggcgaat atgtacgccg taaagaaggt aaaactggta aataatcgtt
    44581 tagaaaaatt gcatagaagc cagcagcagg aaaaaggcat ttggctgaag tttaccttca
    44641 aaaaaataca tttttacttg tgccgcagct tctatttttc gaaacgtatt tactgcttga
    44701 aatagtagta caataaaaat aaagaggtga caattgtgat tacaaaacca gataagaaca
    44761 aaacacgtca aaagagacat cgtcgtgtac gtaacaatat ctctggtact gctgagcgcc
    44821 cacgcttgaa cgtttttcgt tctaacaaaa acatctacgc tcaaattatt gatgacgtag
    44881 cgggtgtaac gctagcaagt gcctctacct tggataaaga aatttcaggt ggaacaaaaa
    44941 cagaagcagc acaagctgtc ggtaaactag ttgctgaacg tgcagctgaa aaaggcatta
    45001 aagtagtagt ctttgaccgt ggtggatacc tttaccatgg ccgtgtgcaa gctttagctg
    45061 aagctgctcg cgaaaatgga ctagaatttt agaagaagga ggaataccat tcatggttta
    45121 tatcgatcca aaacatttgg aattagaaga ccgcgttgtt gcaattaacc gtgtaacaaa
    45181 agttgttaaa ggtggacgtc gtctacgttt tgctgcttta gttgttgtcg gtgacaaaaa
    45241 cggacacgta gggtttggta caggtaaagc tcaagaagtt cctgaagcaa tccgtaaagc
    45301 aattgaagat gcgaagaaaa acttagttga agtacctatg gttggttcaa caatcccaca
    45361 cgaagtaatc ggtgctttta gtggtggtag aatcctaatg aaacctgctg tagaaggttc
    45421 tggggttgct gctggtggac cagttcgtgc cgtattagaa ttagctgggg tagcagatat
    45481 cacttctaaa tctttaggct caaatacacc aatcaacgtt gttcgcgcaa cagttgaagg
    45541 tttgaaacaa ttgaaacgtg cagaagaagt tgcagaactt cgcggcaaat cagttgaaga
    45601 attaatcgga taaggaggac aacaataatg gctgaattaa aagttacttt aaaacgcagt
    45661 attatcggac gtcctcaaaa ccaacgcgat acagttaaag cgttaggtct tacaaagatt
    45721 aacagttcag tggttaagcc ttctaatgaa gcaattaaag gcatgatcaa tactgtttct
    45781 cacctagtgg acgttgaaga aatttaattt agaaaaatca taactagaac attaaggagg
    45841 tgccaaacct atgaaacttc atgaattaaa acctgctgaa ggttcacgcc aagtacgtaa
    45901 ccgtgttggt cgtggtactt catctggtaa cggaaaaact gccggacgcg gacaaaaagg
    45961 acaaaaagct cgttcaggcg gcggtgtgcg tttaggattt gaagggggac aaacaccatt
    46021 gttccgtcgt ttaccaaaac gaggattcac aaacgtgaac cgtaaagact atgcagtcat
    46081 taatcttgat gtattgaacc gctttgaaga cggagctgaa gtaactcctg ttgctttagt
    46141 tgaagctgga atcgtgaaaa acgaaaaagc tggaatcaaa gtattagcta atggggaact
    46201 aaacaagaaa ttaactgtga aagcagcaaa attctctaaa gcagctcaag aagcaattga
    46261 agcggctggt ggatcaatcg aggtgatcta atgtttagac tattaaaaga tgcttttaaa
    46321 gtaaaagaca ttagatcgaa aatcctattt actgtatttg tgttgtttgt atttcgccta
    46381 ggtgcacata ttactgtacc aggcgtaaat gctgcttcat tgcaaaacct ttcttctttg
    46441 ccatttttca acatgttgaa cttggtaagt ggtagtgcga tgcaaaactt ttccattttc
    46501 tcaatggggg tttcaccata cattactgct tctatcgtca ttcagcttct tcaaatggac
    46561 atcgtgcctc gatttgttga atggtcgaaa caaggggaag ttggtcgtaa aaaactgaat
    46621 caagcgactc gatatctgac attagtattg gcatttatcc aatctgtcgg tatcactgct
    46681 ggattcaatc aatacgctca attaggtttc gtgaataatc ctaacgtagc aacttacgta
    46741 acgatcggtt tgattctaac agccggtacg atgtttgtta cttggctagg agaacagatc
    46801 acagaaaaag gtatcggaaa tggcgtatca atgatcattt ttgcaggtat catttctaga
    46861 ttgcctgaaa gtatcagaga aatttatgaa gattacttta taaatattga tccttcagac
    46921 atatggaaat ctgctatttt tgtggccatt ttactagtag cggtcttgtt gatcgtgaca
    46981 ttcgtaacat ttttccaaca agctgaacgc aagatcccga tccaatatac gaaacgcgta
    47041 gctggcgcgc ctacaagcag ctatctgccg ttgaaagtaa atgcagctgg agttattcca
    47101 gttatctttg ctagttcgtt tattgctacg ccaaatgctg ttttacaagc attgagcggt
    47161 agattcggtt ctgaaaactg gtacaaagtg atgatggaga tctttaacta taatacagca
    47221 cctggtgcta cgatttatac gattctgatc gttgccttca ctttcttcta cgcctttgtt
    47281 caagtaaacc ctgagaaatt agcggaaaac ttgcaaaagc aaggaagtta tataccaagc
    47341 gtacgccctg gtaaggggac tgaagagtat gtctcaagat tactgatgcg tttaagtaca
    47401 gttggtgcag tcttccttgg tttagtggca ttgcttccaa tcattgctca aatggtttgg
    47461 gatcttccac aatcaatcgg attaggcggt acaagcttac tgatcgttat cggtgtagct
    47521 ttagaaactg cgaaacagtt agaaggctta atgctgaaac gtaaatatgt tggattcatt
    47581 gagtaagaaa gcgtgttgag gtttcccctc aacacacgtt ctcacacatt tattaggagg
    47641 gaaaacaatg aacctcattt taatggggct gccaggcgca ggtaaaggaa cacaagctga
    47701 aaaaatcatt gatgcttatg gtattccgca catctcaaca ggagatatgt tccgcgcagc
    47761 gatgcaaaac gaaacagctc ttggcctgga agcaaagtct tatatggata aaggtgcatt
    47821 agttcctgat gaagtaacaa atggaatcgt gaaagagcga ctagctgaac cagatacaga
    47881 gaaaggcttc ttattagatg gctttccacg tacgctagat caagcaaaag ctctggacgc
    47941 aatgctaaaa gatttgaata aaaaaattga tgctgtcatc gatatccatg ttggtgaaga
    48001 aatcttggtc gaacgtctag ccggccgatt tatctgcagc aattgtggag caacttacca
    48061 taaaattttc aaccctacaa aagttgaaga cacatgtgat cgttgcggtg ggcatgaatt
    48121 ctatcaaaga gaagatgata aacctgagac ggttaaaaat cgtctggcta tcaacatcaa
    48181 aaatagtgaa ccaatcttgg cttattataa agaacaaggt ttgctaaaca ctattgatgg
    48241 tgctcgtgaa atcgatgctg tatttgcaga tgtacaaaaa atcattgaaa aatagcacga
    48301 ttgcccgtag ccgacttgca ttatgcagtt actcttgtca agttcaataa attatgatag
    48361 aatatgtcag tccgacttct agagaatcct ctagatgatc cattttgaga attttgaggt
    48421 gggaactgca gtttccgcca ttttatcgtg tgaaaatgcg aaacaagtac aaggaggtac
    48481 tgtgcgtggc aaaagaagat atgattgaag tcgaaggtac agtcgtcgaa actttgccga
    48541 atgcaatgtt taaagttgaa ctagaaaacg gacaccaagt tcttgctact gtttcaggta
    48601 aaattcgtat gcattacatc cgtatcttac ctggtgacaa agtaacggtt gaattgtctc
    48661 catacgatct taaccgtggc cgtattactt atcgctttaa ataattgtac tccgtaaaac
    48721 tacaggaggt attatcatga aagtaagacc atcagtaaaa ccaatgtgcg aacattgtaa
    48781 agtaattcgt cgtaaaggac gcgttatggt gatttgccca gcaaatccaa aacataaaca
    48841 acgtcaagga taattggagg tgctataaat atggctcgta ttgcaggagt agatattcct
    48901 cgtgataaac gtgtagtagt ttcacttact tatatctatg gtattggtaa cactactgct
    48961 aaaaagatct tagctgatgc tggtgtatct gaagatgttc gcgttcgtga attaacgaat
    49021 gaacaaacag atgctatccg tgcagaagta gataaattaa aagttgaagg ggatcttcgt
    49081 cgtgaagtga acttgaacat caaacgtttg atggaaatcg gttcttaccg tggaatccgt
    49141 catcgccgtg gattgcctgt tcgtggacaa aacacgaaaa acaatgcacg tactcgtaaa
    49201 ggcccagctc gcgctatcgc tggcaagaaa aaataattct taagtgaagg aggttaaaac
    49261 ttcatggcag caaaaaaagt gaatcgtaaa cgccgtgtga agaagaatat agaagcaggt
    49321 attgcacata tccattctac attcaataac actatcatca tgatcactga tactcacgga
    49381 aatgcattag cgtggtcatc agctggttca ttaggtttca aaggaagcaa aaaatcaact
    49441 ccttttgccg ctcaaatggc agcagaaact gctacaaaag cagcaatgga acacggatta
    49501 aagactgttg aagtaacagt taaaggacct ggttctggac gtgaagcagc aattcgttca
    49561 ttacaagcta caggtttaga agtgactgca attcgtgacg tgactccagt tcctcataat
    49621 ggtgcccgcc ctccaaaacg ccgtcgtgtt taatgagtgc cgctcatttt gagttttatc
    49681 agtaacgtca ttgaacaaga cacttttcgt tttgaaaggg gtaaagataa gaatgattga
    49741 gttcgaaaaa ccaagaatcg caaaaattga tgaagaaaaa gattatggca agttcatcgt
    49801 tgaacctctt gaaagagggt atgggactac tttagggaat tccctacgtc gtattttatt
    49861 atcttctttg ccaggtgctg ccatcactaa tattcaaatc gatggtgtac tgcatgaatt
    49921 ctctaccatc aaaggtgtta gagaagacgt cactcaaatc atcttgaaca ttaaaggctt
    49981 agcgctgaaa atgtatggcc aagaagaaaa aacccttgaa atcgatatta ccggacctgc
    50041 tacagtaact gctggcgata ttatcgttga tagtgatgta gaaattctta acaaagatat
    50101 gtacatctgt agtgtctctg aaggagctac gttccatgct cgcttaacag tgaaacctgg
    50161 acgtggttat gttcaagcag atgaaaataa aaaagaagat atgccaattg gcgttcttcc
    50221 agtcgattca atctacacac ctgttcgtcg tgtgaactat caagttgaga acacacgtgt
    50281 tggacaccgt gaagattttg ataaattgac gatggagatt tggacagatg gttcaattga
    50341 accgttggaa gcaatgagtt tgtctgcgaa aatcatgact gaacatttgg atatttttgt
    50401 gaacttaact gatgaagcaa aaaatgctga aatcatggtc gaaaaagaag aaacacaaaa
    50461 agaaaaaatg ttggaaatga cgatcgaaga gttagacttg tctgttcgct cttacaattg
    50521 tttgaaacgt gcaggaatca atactgtaca agaacttaca aataaatctg agcctgaaat
    50581 gatcaaagtc cgtaacttag gacgtaaatc attagaagaa gttaaagcaa aattgcacga
    50641 cttaggttta ggtttacgta aagaagatta aaacctttta cgaaggaggg aaaccaacgt
    50701 gagttatcgt aaactaggac gcacatctag ccaacgtaaa gcgatgttgc gtgatttaac
    50761 aactgattta ttaatcaacg aacgtattgt gactactgaa gctcgtgcga aagaagttcg
    50821 ctcaactgca gaaaaaatga tcactttagg taaacgcgga gatttgcatg cacgtcgtca
    50881 agctgcggct ttcgtacgta atgaagttgc cagcgtgcgt gaagaaaatg aggacatcgt
    50941 cattgaatca gctttacaaa aactgttcaa cgatattgca cctcgttacg cagaacgtca
    51001 aggcggatac acacgcattt tgaagacaga accaagacgc ggtgatggtg caccaatggt
    51061 tgtcttagaa cttgtctaaa atcacactaa taacatcact ttatagatga ttcttttaga
    51121 gtgttatgat gttggaagat atttttccga gtctagctca gcgctgctcc tcaagcgaat
    51181 atcgcttggg gagtaagcac tcatcataaa gtgttttttt ataccttcaa ttacctgtca
    51241 tgacagatat gtttgaaggc ataaaaaaac attttaagcg agaaataatt gaaaattttg
    51301 tctttttttg atagtgttag taggaacttt aggatgaaga aggagtaatc atggagccaa
    51361 ttattgaatt agaaaagata aattataaat accagccaga tgatcttcgc cccgcattga
    51421 aagatgtctc ctttacaatt gataaaggtg agtggatcgc tattattgga cacaatggtt
    51481 caggaaaatc gacacttgcc aaaacgatca atggattgct tctgccagaa tcagggatcg
    51541 tgaaagtggg aaatcaaata ttggatgaag aaaatatttg gacgatccga cagatggttg
    51601 gaatggtttt tcaaaatcca gacaatcagt ttgttggttc gacagtagaa gatgacgttg
    51661 cttttggtct tgaaaatcaa gggattccca gagaagaaat gctagtgcgt gtgaaagatg
    51721 cacttgagaa agttagaatg gctgagtttg cttcacgcga gcctgctcgt ttatcaggag
    51781 ggcaaaaaca acgtgttgcc attgccggcg tggttgcctt acgacctgat atcattattt
    51841 tagatgaagc aaccagtatg ctagatccag aaggtcgtga agaagtgatc tctacgatca
    51901 agaagatcaa agaagaaagt caattgaccg ttatatctat cactcatgat attgatgaag
    51961 cagctaatgc caatcggata ctagtgatga gacaaggcga acttgtccgt gaaggtacac
    52021 caaaagaaat tttttctgct ggtcctgaac tgatcgactt aggtctagat ctaccatttc
    52081 ctgaaaaact gaagagcgca ctgaaagaac gtggggttga tgtgcccagt gaatatatga
    52141 cagaagaaag gatggtggat tggttatgga catccgtttt gaacaagtag acttcactta
    52201 tcagccgaat acaccatttg aacaaagagc cttatttgac atcaatatga cgatcaaaga
    52261 aaacagttat actgcattag tagggcatac cggaagcggg aaatcaacct tacttcagca
    52321 tttgaatgcg cttgttaagc caacaagcgg tacagtccat attggagaac gagatattca
    52381 gccggatacg gataataaga atttaaagcc catccgaaaa aaagttggca tcgtctttca
    52441 gtttccagaa gcacagctat tcgaagaaac ggtagcaaaa gacattgctt tcggtcctaa
    52501 aaactttggt gtcagcgaag aagaagcact agtcctagca aaagaaacat tagaacaagt
    52561 tgggctggat gaaagctatt tggaacgttc gccatttgaa ctttcaggag ggcaaatgcg
    52621 tagagtagca atcgctggtg tgcttgccat gaggccggaa gtacttgtat tggatgaacc
    52681 aacggcagga cttgatcccc agggtagaaa agaaatgatg gagatgtttt ggcgtcttca
    52741 taaggaacag cagatcacta ttgtattagt tacgcattta atggatgatg tagccaattt
    52801 tgcagattat gtctatgtgt tggaaaaggg acgaatcgtt aatagcgggg agccgcaaga
    52861 ggtctttcaa aacattgaat ggctaaaaga aaaacaacta ggcgtaccta ctgccaccga
    52921 gtttgcagaa gaattaatgg ctaaagggat gaactttgct accttgcctt tgacagctga
    52981 agaattagca gacgctatcg tccattatgc tggaggaaat gcccatgatg aataaattga
    53041 tatttggtcg ttatatgcct ggagattcct tgatccatcg attggatccc cgagctaaat
    53101 tattagccag tttttatttt atcggaatca tttttttagc aaataattgg gaaacgtacc
    53161 ttttgttggc atttttcaca ttactttctg tcttcttgtc aaaagtagat ttgaaatttt
    53221 ttatccgagg cattcgccca ttgatctggc taatcctctt cactgtagct ttgcaggtct
    53281 tgtttacaag tggcggagaa acttattgga gttggggtgt tttccatatc actgaatttg
    53341 gtgtgatgaa cggcctcttt attttctgtc gattcgtgtt gatcattttt atgtcgactc
    53401 tactcacgct tactacaccg cctttggaat tatcggatgc gattgagtac ttgcttcgtc
    53461 cattgaaagc cgtacggttc ccagtccacg aaatttcatt gatgctgtcg attgctcttc
    53521 gttttgtgcc tacattgatg gacgaaacag aaaagatcat gaacgctcag cgcgctcgcg
    53581 gtgttgattt tggtgaagga aacctagttc agaaaatgaa agcagtcatt cctttgctga
    53641 ttcctttatt tgtcagcagc ttcaaccgtg cggaagatct tgcgactgct atggaagcaa
    53701 gaggctacaa aggtggggaa ggaagaacga aatatcgtgt attgcattgg cataatagag
    53761 acacgatggt tatcattgct tttgtattat taactgtgct actgatcttt ttgagaggat
    53821 aaacgttcag tgggccgcta tctattcttt tcttgctgat ctgtttgcaa gaaaggatgc
    53881 gagcagtgta tgacaagaaa tattagggtc tgtggcagac taaggtcaca ggcctctttc
    53941 ttttatctga aaagaaagaa cgaaaaaatt ttataaaaaa atcattattc cagataacct
    54001 tttaggaaca aaggagagaa aaacatggta cgttacaaag caatcattgc atacgacgga
    54061 acaaatttta atggattcca aaaacagcca aatgggcgta ctgttcaaga agaagtagaa
    54121 aaaacattac aaaaaatggc aaatggaaaa gaaatcacgg tctttggttc gggaaggaca
    54181 gatgccggag tacatgcgat tgggcaagta atccattttg attatccaga ggagcgtccg
    54241 ttagaacgaa tgcgtttcgc gctagatacc cagtcaccag aggatattgc tgtaagacaa
    54301 gtagaaattg tttcagaaga cttccacgca agatatttgg tcaaagaaaa gacgtatcaa
    54361 tttcgagtgg atatagggaa accacgcagt ccttttcgac gccattatgc aagttatttt
    54421 ccttaccctc ttgatttgga aaaaattcag cgggcgcttc ctgatttatt agggactcac
    54481 gatttcacct ctttttgtgc atcaggaagc tcgatagagg ataaagtacg tacgatctac
    54541 gaggctaaga tggaggtaaa tgaaacaaaa gacgaattgt tgtttacatt taggggaaat
    54601 ggcttcttgt ataagatgat acggatctta gtaggaacgc ttttgaaaat tggcaatggc
    54661 cgattgcctg aagatagtat cccagatatt attgcgaaaa aagatcgtaa tgcagcagga
    54721 cctacagcac atcctgaagg actttatttg tatgaagtga aatatgaata agaggttgtg
    54781 acagaagtgg tcagcttcaa gaaataagaa ggaatttacg aaaattactt ctcaaaattt
    54841 tgcaaaattt cggcttattt tcgaaggagc tacttctgga acaccgtgaa tcagaggttg
    54901 tgaaaaagct gccctgcatc aagtaataag aacaaccaaa caaaaatagc tcgtcatatt
    54961 tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt taaggaagag
    55021 gttgtgaaat cagcgttttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    55081 tattttgccg tttgttgagc taataccgaa ggtcagctga ttgaacaccg tttaagaaag
    55141 aggtcgtgag cctgtcattt aactctgagt cacaagtaac aatttcaaaa aacagcttct
    55201 catctttgat gaatatcgtg acttgtggac gaggcgttga taagtgaatc atctttttgt
    55261 tacctatttt ttctgtaacc aaaaaacgat ggaacgttgt tttgattgcc cttaatgagg
    55321 acagtcggaa caggtttgtc atttttatat ttaaatggca caaatttttc taattatttg
    55381 tcatttagcg ctgaaaattt ttgctattta tctaaaaaag tgataactac ttaagatgaa
    55441 gcagaatatt tacgccatac ggcatacaac cgcgaaaaat ataaaaaaaa gaaaacgatt
    55501 gaacgcgttt ttgcagtcgc aaaggaaaag catggaatgc gctacatcaa gtatcgagac
    55561 ttggaaaaca atcaggtgta tacgctgatt gtttttgctg caatgaatct gaaaaagttg
    55621 tccacttggc agttggaaca tatgtcttca tatcttttat tatacaaaaa tagcaaaagc
    55681 accagtctac actcttagta aagaggaaac tgatcctttt gtctacattc ttaaagtact
    55741 taaactaagt actttatttt actctttagg attgatacag tagcatctat ccctttatta
    55801 ataatctttt atttttttaa ataaagatgt tgtctccatc ttcaggcact aaaacttttt
    55861 cggaaattcc tttttcttta ttgaaagact ttaattcttc tcttgacagt ccccaatggt
    55921 ttacagcttc catatgaacg gcaataattg tggaatctgg tattgcttta tgcaattcat
    55981 ataaatcgtc tttccccata ataagagatt ctccaactaa aaaatgatta tctccagcat
    56041 ttgctactat gatttctgga ttatactgat caatagtttg tttaacagct tcataccaaa
    56101 ctgtatctcc tgtaaagtat agtgtcttct cagtcgaatg tttgaaaaca ataccgcaaa
    56161 cattacctac ataattcaag atttctcctc tgccatgttt gccttttgtt ttaaccaatt
    56221 gtatagattc aaaaactgtg tcttctcgta gtacctccac atttttaaaa ccatatagtc
    56281 gaatttcatt cgcatcattt tcatcttgca caaataattt tatttcttta ggtaggacct
    56341 ctttcgctat atcgtcccaa tgatctagat gtaaatgtgt tacaataaca gcatcaatac
    56401 cgttgataat atcttcgact ggcattggca aatcaactag aggattcatt tgatcttttc
    56461 ttggtgatgg aaaaggacct ttcgaaataa atggtggaag tgttcctttt tccgctaaca
    56521 ttggatcaat taaaaatttt ttacctccat agtctaagat agctgttgca ttacgaatgt
    56581 gatgtatatt cataaacgtt gtctcctttt tgttgatatg aaatattttt agtataattc
    56641 aattccatac aataaataaa ccgaacaaaa aaagtctttt actattttta cttaatttat
    56701 gaaaggttgt ttaccatgtt agaccaaaca gatataaata tcattgagga actatcaaaa
    56761 aatagtcgaa tcacaatgaa agaacttgga gaaaaagtac atttgactgg acccgctgtc
    56821 tcggcgagag tgacaaaact agaggaaagt ggagttattg aaaattatac agtaaaatta
    56881 aatatggaaa agctagggtt ttcgattcac gcttttttaa ttataattac gcaaaattta
    56941 aatcataaac cctatttatc atttattaaa aatcatgaaa aattcttgat tagaaattat
    57001 aagattagtg gagggggctg ttatcttttg gaatgtagat ttacttcaaa aaaatcaatg
    57061 aacagctttt tggaacagct taatgaatat gcgaattata aattatctat tgttatagat
    57121 taataataaa actagcagat ggtaaaaata ctaatatgcg agacacacat tcaaccataa
    57181 ctatataact tatcaattta aaactaaact gatggaaaca agtaaggcaa aaacatttat
    57241 attttgccct acttattttt ttaattaaat tcattgatga ttcatattta gaaaattcta
    57301 tgaaggccaa atgagtatcc t
//
LOCUS       DAZKOR010000007        56250 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000007 DAZKOR010000000
VERSION     DAZKOR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.007"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1612)
                     /locus_tag="ACG8ZM_000359"
     CDS             complement(44..1612)
                     /locus_tag="ACG8ZM_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0491353.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1616..2026)
                     /locus_tag="ACG8ZM_000360"
     CDS             complement(1616..2026)
                     /locus_tag="ACG8ZM_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0491354.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2023..2946)
                     /locus_tag="ACG8ZM_000361"
     CDS             complement(2023..2946)
                     /locus_tag="ACG8ZM_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0491355.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2943..3821)
                     /locus_tag="ACG8ZM_000362"
     CDS             complement(2943..3821)
                     /locus_tag="ACG8ZM_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0491356.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3808..5145)
                     /locus_tag="ACG8ZM_000363"
     CDS             complement(3808..5145)
                     /locus_tag="ACG8ZM_000363"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0491357.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5148..6251)
                     /locus_tag="ACG8ZM_000364"
     CDS             complement(5148..6251)
                     /locus_tag="ACG8ZM_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0491358.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6325..7833)
                     /locus_tag="ACG8ZM_000365"
     CDS             complement(6325..7833)
                     /locus_tag="ACG8ZM_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0491359.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7799..8491)
                     /locus_tag="ACG8ZM_000366"
     CDS             complement(7799..8491)
                     /locus_tag="ACG8ZM_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0491360.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8513..9487)
                     /locus_tag="ACG8ZM_000367"
     CDS             complement(8513..9487)
                     /locus_tag="ACG8ZM_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0491361.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9787..10659
                     /locus_tag="ACG8ZM_000368"
     CDS             9787..10659
                     /locus_tag="ACG8ZM_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0491362.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11117..13315)
                     /locus_tag="ACG8ZM_000369"
     CDS             complement(11117..13315)
                     /locus_tag="ACG8ZM_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0491363.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13547..14539
                     /locus_tag="ACG8ZM_000370"
     CDS             13547..14539
                     /locus_tag="ACG8ZM_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0491364.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14599..16020
                     /locus_tag="ACG8ZM_000371"
     CDS             14599..16020
                     /locus_tag="ACG8ZM_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0491365.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16013..17512
                     /locus_tag="ACG8ZM_000372"
     CDS             16013..17512
                     /locus_tag="ACG8ZM_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0491366.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17551..18945
                     /gene="uxaC"
                     /locus_tag="ACG8ZM_000373"
     CDS             17551..18945
                     /gene="uxaC"
                     /locus_tag="ACG8ZM_000373"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0491367.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19104..19787
                     /locus_tag="ACG8ZM_000374"
     CDS             19104..19787
                     /locus_tag="ACG8ZM_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0491368.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19789..20625
                     /locus_tag="ACG8ZM_000375"
     CDS             19789..20625
                     /locus_tag="ACG8ZM_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0491369.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20636..22390
                     /locus_tag="ACG8ZM_000376"
     CDS             20636..22390
                     /locus_tag="ACG8ZM_000376"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491370.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22666..23007)
                     /locus_tag="ACG8ZM_000377"
     CDS             complement(22666..23007)
                     /locus_tag="ACG8ZM_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0491371.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23075..25246)
                     /locus_tag="ACG8ZM_000378"
     CDS             complement(23075..25246)
                     /locus_tag="ACG8ZM_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0491372.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25457..26314
                     /locus_tag="ACG8ZM_000379"
     CDS             25457..26314
                     /locus_tag="ACG8ZM_000379"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0491373.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26385..27242)
                     /locus_tag="ACG8ZM_000380"
     CDS             complement(26385..27242)
                     /locus_tag="ACG8ZM_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0491374.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27227..29929)
                     /locus_tag="ACG8ZM_000381"
     CDS             complement(27227..29929)
                     /locus_tag="ACG8ZM_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0491375.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(29942..31231)
                     /locus_tag="ACG8ZM_000382"
     CDS             complement(29942..31231)
                     /locus_tag="ACG8ZM_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0491376.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            31381..32427
                     /locus_tag="ACG8ZM_000383"
     CDS             31381..32427
                     /locus_tag="ACG8ZM_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0491377.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            32429..34582
                     /locus_tag="ACG8ZM_000384"
     CDS             32429..34582
                     /locus_tag="ACG8ZM_000384"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0491378.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(35055..36506)
                     /locus_tag="ACG8ZM_000385"
     CDS             complement(35055..36506)
                     /locus_tag="ACG8ZM_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0491379.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(36503..38227)
                     /locus_tag="ACG8ZM_000386"
     CDS             complement(36503..38227)
                     /locus_tag="ACG8ZM_000386"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0491380.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(38220..38834)
                     /locus_tag="ACG8ZM_000387"
     CDS             complement(38220..38834)
                     /locus_tag="ACG8ZM_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0491381.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(39179..40636)
                     /locus_tag="ACG8ZM_000388"
     CDS             complement(39179..40636)
                     /locus_tag="ACG8ZM_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0491382.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(40677..41597)
                     /locus_tag="ACG8ZM_000389"
     CDS             complement(40677..41597)
                     /locus_tag="ACG8ZM_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0491383.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(41609..42556)
                     /locus_tag="ACG8ZM_000390"
     CDS             complement(41609..42556)
                     /locus_tag="ACG8ZM_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0491384.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(43035..43754)
                     /locus_tag="ACG8ZM_000391"
     CDS             complement(43035..43754)
                     /locus_tag="ACG8ZM_000391"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0491385.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(43803..45449)
                     /locus_tag="ACG8ZM_000392"
     CDS             complement(43803..45449)
                     /locus_tag="ACG8ZM_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0491386.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            45628..46218
                     /locus_tag="ACG8ZM_000393"
     CDS             45628..46218
                     /locus_tag="ACG8ZM_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0491387.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            46310..47815
                     /locus_tag="ACG8ZM_000394"
     CDS             46310..47815
                     /locus_tag="ACG8ZM_000394"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0491388.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(47899..49251)
                     /gene="celB"
                     /locus_tag="ACG8ZM_000395"
     CDS             complement(47899..49251)
                     /gene="celB"
                     /locus_tag="ACG8ZM_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0491389.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(49251..49769)
                     /locus_tag="ACG8ZM_000396"
     CDS             complement(49251..49769)
                     /locus_tag="ACG8ZM_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491390.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(49785..50111)
                     /locus_tag="ACG8ZM_000397"
     CDS             complement(49785..50111)
                     /locus_tag="ACG8ZM_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0491391.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(50124..52103)
                     /locus_tag="ACG8ZM_000398"
     CDS             complement(50124..52103)
                     /locus_tag="ACG8ZM_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0491392.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(52124..52438)
                     /locus_tag="ACG8ZM_000399"
     CDS             complement(52124..52438)
                     /locus_tag="ACG8ZM_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0491393.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            52606..52899
                     /locus_tag="ACG8ZM_000400"
     CDS             52606..52899
                     /locus_tag="ACG8ZM_000400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0491394.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(52977..54089)
                     /locus_tag="ACG8ZM_000401"
     CDS             complement(52977..54089)
                     /locus_tag="ACG8ZM_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0491395.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            54242..55090
                     /locus_tag="ACG8ZM_000402"
                     /pseudo
     CDS             54242..55090
                     /locus_tag="ACG8ZM_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(55443..>56250)
                     /locus_tag="ACG8ZM_000403"
     CDS             complement(55443..>56250)
                     /locus_tag="ACG8ZM_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0491396.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPI
                     EFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLT
                     TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        16768 a        11505 c         9909 g        18068 t
ORIGIN      
        1 tagtgaaaat aagttagtga acctcattta ttaaagaatt aaattacaga tgatcttttc
       61 catcatactt gaaaaactca aaatcagcat aacttcctct ttttttattt agatcatgca
      121 ctgctatccc gataaaattt ccagtaaacc ctcctgagag tatacacatg tttttttgcg
      181 gacctacttt cttccattct tcagttgata cgtctttcaa atgaaactgt gctgtctctt
      241 tctttacagt gactttcaaa aaagtcttct tttctgtgag tggaatcgtc caatcttcta
      301 tttgtgctac tccgttttct gattctatca tacgtaggca ttttcccttg tcctcattcc
      361 aagtaacata acaataaata taattggatt catttaagta cagtaccagt ccagccatct
      421 gattaaaatt attaggcatg tattcaaatg ctgtctccgc atcaaaagta aaatccgttt
      481 gccgaatagc taacaaatga tgttcgaagg tagattgaag agattcaccg gaaatcagac
      541 gtaaaaaacc tggtctacat tttgtatcac accaatcagc atttggcaaa atgcgtctgg
      601 cattccagtt cttctttagc tctccttcaa atgtatccat aaactgatga tctttttctt
      661 gttgtacctc ttcatttgtc tcaatcatta tttcttccat cggagttgtc tggctatctt
      721 taagtcttaa ccaatcgtcg gacgtccaat acacttcttg aatagcggtt tctcttccca
      781 aaatagccgc accgtttaat ggtctggtac aaagatatgt catgtaccat cttcctgacg
      841 gggtttgtac taagctccca tgtcctgtac attgcaatgt agaagcaggt ttgtcactag
      901 ctgtcagcat aggaaaacca ggatcaagct cgtaaggacc agtaatattc tttgctcggc
      961 aaactgtaac tgaatgtcct cttcctgtac cgccttctgc cgtcatcaaa taataatacc
     1021 cgttacgaaa atacaaatgt ggtgcctccg ttttcgctaa ttctgtacct gcaaatattt
     1081 tatagatggg accatcaagc ttcttcttct ctgcatcata ttcttgtaat acgataccgc
     1141 tggatttatt cggtgtgtcc agacgataat cccatagttc attgacaagc catttttttc
     1201 ccgtttcaag atcatggaac aaagaaggat caaagccgtt actatttaaa taaattggtt
     1261 cggaccaagg acctgtgatc tcagatgcag tgatcaaata attgtggaca tctttaaacg
     1321 gacggcctgc acttttgaca tctgtgtaaa caagataaaa caatccatca gcataactga
     1381 tttgtggcgc ccatatactt ccatttgtag gatttccttg caaagatact tgagtggtca
     1441 aaatatccgt tgtatgtgac cagttgacta aatttttcga ctcatatact cggattcctg
     1501 gaagccattc aaaagaggag acaataatgt aataccgctc tttcactctt aatatattag
     1561 gatctggatt gaatcctctt aaaataggat ttttcacttt gatagtctgc atgattcagg
     1621 ctccttttct cattcttact cgatataaac tgaatgcccc agctgcgaaa taaaaaaatg
     1681 caaaaggaaa cgcaataaac tgagtgccaa tcaacaatat gaatcctgtt ataaaataca
     1741 aaccagtggc agttgttaag tattttcctt tagcagtcaa aaacaatgct actgctaaaa
     1801 aagcaagtga aataaacaac acaacggcaa acagaccagt catgtcctgt aataatccaa
     1861 atgtattttc taaagacaaa gttgaatgat ctacagcaag cgactgataa ctttttttaa
     1921 actctgacgc agacatatta cttaaagtaa ctgctaaccc gccaaagaaa atcaaagata
     1981 gactgccacc gaccattcct agcaatagtt cccattttct tttcatttct gaaccccctg
     2041 cttttctaaa tgtatccatt caggtctttt gatcgatgtt tcataattgt tattacgttc
     2101 agagaatttg acagtctgtt catttttctt gtcatcccaa ttatcccaac cagttgggtg
     2161 aacatgccca cctatcttac acgcttcaaa taagacattt gcatacggac gccacggtct
     2221 tcctaaatag acgtttctag tatcaggttc tgcagtcaag gtacagcttt caaaacgata
     2281 ccctgttttt tgatttttcg gcgtagatgc tgctgtgatg tatccaggat ttttactggc
     2341 taagctatgg atatgacact gatgaaaaac ggcatctgcc ccaccaaata tgaaatccac
     2401 cgttccttca atccagcaat attcataggt ctgacgacaa tactcaggat tggcagaagc
     2461 cggtgtacaa aaaggagtac catctttttg caaatcaggc aacggacccg tgcataaggt
     2521 atcctgataa ccagatagtc gacagttttt taaagatacg cgatctcctg aattgaataa
     2581 agcaacagcc tgtcctacct tttctcctgg acctgctgta ttttcgatgt gaagattttc
     2641 taatacgaca ttcgatcctt ctaagaaaaa agtagcactt cggaaagtcc ctaactggct
     2701 accgtcaaga tactcctgtt ttgcaaaacg attaccggag ataactactg ttcctgctcc
     2761 cacaagttta aaattggata atctgctttc tatatattca tcatatcttc cttcttttac
     2821 tatcaagacc tttggatggc tatccggcag gcctgccaaa taatcgattc cttcttggat
     2881 agtttcaaaa tccgtcgggg tatctatagc attctttcca attatcagtt tgtctttcat
     2941 cctcataaat gctcccttat gaattgtttc caaatatttc tcatttcttg tgtttccata
     3001 tgaccgcctg ttaaagaaaa agaagaaaag ttttctggac tacccattga ttgatacttt
     3061 ttgtccagcc cttttctcag cttcagtacg ccttcgtcca agcacatcgt atcgtttttt
     3121 ccaactaagc taagacgagg gcgcggagcg attttttctt ggattgctaa cgttgtataa
     3181 gctgtcaata gatttggcac atagtaataa aacccatgat gatctaggcg gcgatgtagc
     3241 aaaagtattt cgatgtccac ctgtgccgct agatcaatac aaactttgac acgagaatcc
     3301 aatgctgtca cccaccagct taataagcct cccatagaaa gaccaatcgt tgcgagccgt
     3361 tgagtatctg tgtcttccct tgtctccaaa taatcgatca agctgatcac atcataaata
     3421 cgcattcccc ataacgtttt tcctgttaat aaaaattgtt tgaacaactc gctttcagaa
     3481 atacctccgc gttcttcaaa tccccaagca tcaatcgacc aaacagcata tcccattcct
     3541 gtcagtactt ctgcaaaaga tggagaagct aagtaaggag caccatggat caattcactt
     3601 ttcccgactg aaaaatctcc gccgtgagaa tgcaggtaaa ggaccggcgg aaacggtcct
     3661 tctttttctg gataagcata gagggctggt actgtttcca attcatttaa gtacaattga
     3721 tagtattcaa gcataaaacc gttttgtttt tctcggtata atcgttttcc actaggggta
     3781 tgtctgctag atggagcacc taaaaaatca ttcaactgca taagcaaagt ttttacgaac
     3841 aaccagttca tccatatttt ctgattgatt tgattggaat ttctcaatac tgatttcttt
     3901 agtattttct aatagaaatg ctggtccctc gtgattttca atcgacatct ggttaaagga
     3961 aaccttaccc gcaaagccaa tataaaatcc ttgtctgctc atttcctcta ttcctgtcat
     4021 catagcagga acaccgggaa ctgcatcttg ggccatcgag acatcgatat tgtcaaatgt
     4081 gatttcttgt atgaattgtt ccgctaatcc ataaataaaa cctgcagaag catggacatt
     4141 ccttgccgtg atattcgaaa aatggatcct tctgaatgct ggtgtccgtt catctatcgg
     4201 ataagccttt ttttcccata catacggttc tttccctcgc ggaccacaga aataataaag
     4261 attcagtata aatggacaca tcacattatc catgactata ttggatactc gaatatcttc
     4321 aacgatcccg cctcgacctc gtctagattt caatcgtatt cctcgatcag tttcttggaa
     4381 gatacaattt gaaatggtaa tattacgaat actaccactc atttcacttc ctaatacgac
     4441 accgccatgt ccatggacca tcgtgcaatt tgtgatcgtg atattttcgc aagcgatcct
     4501 ttcgtacgta tcttcagtcc ctgccttgat tgcaatacag tcatctccca cgtcaatatg
     4561 gcaattacta atacgcacat ttttgcatga ttctggatca attccgtctg tatttggtga
     4621 atccgctggg ttcaagatcg tcaagttatc aaacgttgca ttactgcaaa gaatcgggtt
     4681 gatcgtccaa cttggtgact ggatcaattt aatatctttc acagtaattc gatgacagtt
     4741 atggaaactc atcaatttag gacgaggata agcgagatta tctggttcat ttctaaaagt
     4801 atgccaccat ttttttccat ttccatctaa tgtaccgaaa ccagtgacag agatgttctc
     4861 tacattttga gcatagatac aggaagcata aacttttcga tggactcctt cccaacgtga
     4921 atgaacaaca ggataatctt ttggatcgtc agagaatttc agaaccgctc ctgcgcttaa
     4981 atgaagttcg acattgcttt ttaaaaataa agctcctgtt agaaattcac ctgctggaac
     5041 aactacggtc ccgcctccgt cacttgctgc agcatcaatg gcttgttgta ttgcttcagt
     5101 attcaattca tcaattgatg cgccaaatgt caaaatatca tacatattta taatgcctcc
     5161 acttcaatca atgcttgcaa gaatgcacca acgccttttt gatcattaca aataattggt
     5221 tcactgatgt agtaagcata tgaaccatct cgctgatctg ctccgcctaa ccctgctact
     5281 tgacagtttt tattcaagtt cagccagcct tcttttgttt ccagaacaaa ttcatcaagt
     5341 aatccttgat gtgatttctt cacttgtgga agccattctt ctttgttcag cagtcccaaa
     5401 cgtattcctt tagccattgc gtaaacaaac atactactgc cggaagcttc cagataattt
     5461 cctttccgtt ttgtttctgt tgttacttga taccatgtcc cgctgttttc ttcttgatat
     5521 ggaagtaatg cagccagtgt cttttctaga atttctttca gcaaagaaca atctgtttct
     5581 tcatgaagta attcataggt atctgctgca gccatcaaat accagccaat cgatcggctc
     5641 caaaaatgat gggatagacc tgtttttgga tcagcccatg gttggacctt cttttcatcc
     5701 catgcatgaa tcagcaaatt tgtttttgga tcgaatagat gtttataact gatttcaaac
     5761 tgtctcaaaa catcttggta atctttttct tcactaaaat tctggataaa ttcagcataa
     5821 aaaggagaac ccatatataa gccatccaac caaatttgat aaggataaat ttctttatgc
     5881 caaaaagctc cttcggatgt gcgtggatgg tttgccaact gctgggaaag gagttctgct
     5941 gcttttttat atttttcttc gcctgtttct ttgtacaata aaaagaaaag ctttcctgta
     6001 tttacatggt cgatattgta ttcttcaacg ctgtagccac ggatcgttcc atcttcttgc
     6061 acgaaatagt cgatattgtc ttttatgaaa tcaaaataaa ttttttcttg ggttcgttcc
     6121 caaagtaatt ggaacccttt aagtacgacg ccatagtcat aagaccattt tcctttatac
     6181 cacttttctt catataagcg aggtgttctt tccatgatac tttttgctaa tttctttgac
     6241 cagttttcca ttatttgctc tcctttaaaa attaaaatag gatttgggac aaataccaag
     6301 aagtatttgt cccaatcgtc tatattactg tgccgctgct ttgtatgctt cctcgtactc
     6361 ttgtgtgatt cgatcgcctc cttgtgcttt ccagtcttct acagcttttt tgaatccatc
     6421 ttcgtcaagc tgtcccatga tgtattgcac cgtagcatca atgacgattt tttcaatttc
     6481 tgatcctact tcattgttcg tcggtgaatc caatggcaca gaaggatcca ttacagcaaa
     6541 ttcttcattt tctttgatca attgattaga gaattctttt tcagggtctg tatctttgaa
     6601 agagaacgtc acttcactag gacgagagct tgataacggt tgcacctctt gttgccaaag
     6661 atccatatcg ataaattcga tagaaccatc atcattcaat tcataatgcg ttccttcgat
     6721 accgccagtc atcaatttat acacatcttc ttctaaaaga tcatcaacaa attgaagtaa
     6781 tttcttcaat tgtgcttcgt cttttacttg agatttggga aatgctagca agccgccaat
     6841 accatttcct tctgaccaaa catgataacc gccgccttca ccatctgaga tcttgtttac
     6901 aggcactaat tccatatcat cttgaatacc ttgacccatc gtcttcagat tcttgatatc
     6961 caccattcca gtgtagattc cagttcttcc ttgtgcaaat tgttcttgct gatcagtctt
     7021 ttgggtaact gcaaagtctt gtgccaaata gccattctca aatagatctc ttgaaaaatt
     7081 caaagctttc atatactctt ctgtatcaaa ttctggagtg aatttgccat tatcgtctac
     7141 agcccagcca tttggtgctc caaaataaga agtgaaaagt ttaaagctgg tataacgaat
     7201 atcactaggc gcacgatcgc caaaaccaac tgtgtcgtcc acaccatttc catcaggatc
     7261 gttttctgta aattgtttag ctacttcata aagctcatcc atggtagttg gaacatctag
     7321 tcctagatta tccaaccagt cttttcgaat gactaagcca gcacgagcgt agtctttttg
     7381 tatcggtacg ccgtacaagg caccgtcgat actcgcagct tcgattctat cttcagaaat
     7441 ttgacttaaa tttttatagt cttttaaata ttctgataca tcccagaata cgcctgattt
     7501 taacgcattg cggacagaag agtttttcaa catcgcaatt tgaagtgtaa cgatatctcc
     7561 tagttcatca gaagccaatg cagttgttaa tctttcttct tttgaagcat caggaatcca
     7621 gttgaatgtc agttccgtat cggttttttc ttcgatcaag ttcacaatcg tgtctgtagg
     7681 cggtgaagca tgatgcaaaa tatttaacca agtgatttta gcttttccat cttctgtctc
     7741 tgaagaactg ttacttgaac tgctgccgca tgcggcaagt gctagaattc ctgcaaaact
     7801 aagacctaat tttacgagcg ttgctttttt cataaatttt ctccttcact gttttaataa
     7861 tatttttgtt ttcttctgtt ttttctgtat atgagttctt ccattccaaa tggcaaaata
     7921 tagaatttgc tgtccacgtg actgcggaaa agaagagact gccaccgata aaggttaaaa
     7981 cacctggcat tgtttggatg atcatcagat aaatccctac catacttaat atcaacacta
     8041 atgagtaatg aagatgtgag aatccgaata aaaatgccat tttcattttg aaaaaatatc
     8101 ctttccaatc ataatgagcc atgattggga aaatatagag tgctgagagc acatatatga
     8161 acgcagcaat atacaaagcg atcctaagat accaattgtg tatgaaagaa agatccacaa
     8221 gtaaaacaat cccgatggcg ccatatatcc agctcatcag caaggtttca cgatagtttt
     8281 ctttaaaata tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag cgcagccatt
     8341 ttctaatcac tgcaaccatc gcatacgatg caggaccgaa agtaaaaact cccaaacctg
     8401 cgatgactaa accgccccac agaaaattca aataaaccaa ctgtaaaata cgatttagcc
     8461 ttccgtttat tttatctaat gactgcagca tatctttccc tctctttcta tcttagtaaa
     8521 cgccgtcttc tcctagcttt ttggcaatct tattagctaa aattaccata aacaatccta
     8581 cgaccccttt aaacaatccc attgctgtac tgaaacttaa ctgtccattc ttcaatccag
     8641 ctgtataaat ataggtgtcg aaaatttctg caacgcttcg attcaatgaa ttcaataaca
     8701 agtacatatg ctcgaaccct aagtctaatg tctgaccgat ttttagaatc agcaaggtaa
     8761 tgattactgg acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc
     8821 catccatttc tgcagcttca tacaactgtg tatcgactgc agttatcgct gaaaggtaga
     8881 tgatcgtaga ccagcccaac tctttccaga tcacttgtcc gatatatact gtgcgtgtcc
     8941 attcagggga tgttagaaaa ctgatttttt caaatcccat tgttgcaagg aattcattca
     9001 atacccctcc gtcaacattc aaaaatacat aagtaatcga aacaatgatt acccaagaca
     9061 tgaagtgagg gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg ttcgttactt
     9121 catttaatag taaagacaaa atgattggca tcggaaaaaa cacgaagata tttaatccga
     9181 acaagatcaa cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga
     9241 aatgtttgaa accgacccaa ggacttccca ttattcctaa aaatggttga taatcttgaa
     9301 aagcaataac gagtcccccc ataggaagat acctaaaaat caagaaataa aggactcccg
     9361 gcagaatcat cacatataaa aatttattcg ttttgattcc tgcaattatt ttttttcgtc
     9421 ttctgagctt ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt ttctttacac
     9481 tttccataag cccctcctta gttttgtgaa tgtttgctgc ttatgacttc atgatagata
     9541 actatctgaa ttacgtatat aatcattttt accgtaagta aaaacgctta catagcaatg
     9601 atttttgtgc tatacaaaac gataaagtgt attttttcac aaataaacta cgcatagcaa
     9661 accttctatt tttttctatc tgacacttag cataggaaat gattatatac gtaatctttc
     9721 accgatgata aattgaacat atagaaacag aaacatttaa tttattttga ggaggagcct
     9781 atctatatga aaaagaaagt ctctatagga gaaagaagtt ttacactttt caacaatacg
     9841 tttctaattt tgctagcttt gatctgtatc gttccttttt taaatatcat cgctacatct
     9901 tttgcttcta cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac taccttttct
     9961 ttagacgcgt atcgctacat tctttctacg ccgaccattt ttagagcact tgctgtttcg
    10021 attggtgtta caggtgtcgg aacgatcgtc agtatgtgtg caacttcact gatggcctac
    10081 ggtctttcca gaaagtattt gtttggtcga ggtttcgtca actttcttgt tgtattttct
    10141 atgttgttca gcggggggat gattccaacg ttcttagttg ttcgttctct agggctggtc
    10201 aattcttact gggccatgat cctgcctgtg gctgttaatg caatgaatat gatcattatg
    10261 agaaactttt tccaagcctt gccggatagt ttggaagaat cagcaaaaat ggatggttgc
    10321 acagattttg gtgtattttt caaaattatg ctgccattag cgttaccgtc tattgctact
    10381 atctcgcttt tttatgctgt tacttactgg aacacttata tgactgctat tttgtatatc
    10441 aacgattcat ctaaatggcc gatacagatt cttttacgcc aaatcgtcat tgtatcaagt
    10501 gggatgcaag cagaaagtag tgccgttgat gtgattccac cagcacaaac agttaaaatg
    10561 gctgttatcg ttattgcaac agtgcctatg ctgattgttt atccttttgt tcagaaatat
    10621 tttgtcaaag gagcactcgt aggttctgtc aaagggtgat tatgtactga acgcaaaatg
    10681 gtagttgtgt cagaagtggt cagcttcaag aaataagaag gcatttacga aaattgcttc
    10741 ttaaattttt gtgaaatttc ggcttatttt tcgaagaatc tactcctgga acaccgtgta
    10801 tcagaggttg tgaaagagac gttcagcctt gagcgatgat aaaaactctc acaaacattg
    10861 cgacgagtaa cccacaggag caataagaag cgattttgaa aaatagcttc ccatatttca
    10921 caaaattatg ccttattgtc gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga
    10981 actgtgacaa gacttttgtc acagttcttt ttgcgtgctt agccttgaaa tttaactatc
    11041 cataaataca aatactaggg ttattctaac ttcttttatt gcttgaatca gtccacaaga
    11101 aaaacagcct cttgattcac aaatattttt ttcgatattc tcccggcgta atcgattcct
    11161 tctttttaaa gaaacgaata aaattttgcg gatttcgata ttgcaatctt tctgcaatct
    11221 cttttacgga gaggttagtc tctttcaacc atttttttgc tacctctaaa cgatgatttt
    11281 gaacaaaatc accaaatttt tctcctgttt cctttttaaa tacattactt aaatagttag
    11341 ggttataatg caacctatca gcgatcacat ctaaagacag atcacgatca tattctgtgt
    11401 agacgatatg cataatattc tccgacaaat ttttaaattc atgatcagtg attgcttgag
    11461 agcattctgt gattggcaga actaattgtt ctaaaacaag tttttctagt tctttgggat
    11521 gataattgtt cagtgctttt acataaattt gtttcatgtt atcaaagttt tttgaatcta
    11581 ctcccaataa ctgtcctaat tgaacgagtt cattgatcaa gcggaccgaa actacttccc
    11641 gactcaaagg atttttattt tgtgcaaaca atgcatcaat caattcatgt actaaattct
    11701 tcacttcctg atttccagaa cgaatggctt caaacaatcg attttgttct tctatcggat
    11761 atctaataag tgttttttca tgagcagcag gaacgatttc ctgataaaaa ataattgaac
    11821 ttggaccagt atttacacga taatacaggg cttcttttgc ccgatcaacc gattgcttgc
    11881 tttcattcag cgtttcgaac cggttactga tgcctatact gatagttaaa ttcaagtatt
    11941 cttttacacg tttttgaatg agtcggcaat actccatgac ttttttgctt gcttctgcat
    12001 catctttatc cattcgataa atagtggctt gcatttcttc attcaaaacg atcggcagca
    12061 ttcgattttc ctttggcaca atttccgtga tcatgttgtt gatcgctaaa agtagcaaat
    12121 cacgctcccc accgtgacta tcttctaaca gatcgatttg aaccaaagct gtataatagc
    12181 agtcattttg agaactataa ccaaattgct gcaaccgctg atctagttct cgcctttcta
    12241 ctcgatttct aaataaacta agtacaaata gcgtttccag ctgtggcttt tgggtaacta
    12301 agtgtgcact taacgcttct ttttctccta tgatccgact gactgattga gcgaccaaat
    12361 ctagttcttt cccactgaat ccttttgatt taagattcag acgttcctgt atttgagaaa
    12421 tcggacgggc aaatctgtcc gaaaataagt aggaaagaag accaatgagt cctaccagtg
    12481 ttaaagacac tgcaactagc ccgattctca gattgcggat cattgcgcca attgcactag
    12541 agtcaacttc taatctgtaa atccaattat tataatctga tttcagtact atatacgttt
    12601 tatctttttc tcttatcact ttcagctgat tgtaatctag atccacgtcc gttaatacag
    12661 ttggttgctc tttatttttt actgtctgag tagtaaaaag gttgccaact tcgttttcaa
    12721 tagaaagtat gtcttctcca ctttcaccaa caacttgttc tattgtatgt ttctctatcg
    12781 tagcaattcc caatgcatat ttttcttttt caaacattgg caaagaaatc accatttcga
    12841 ttcctttgtt tttcttttgc caaaataaat tatttttgtt atttatgtac ttctgttgat
    12901 acttttcaac ttcctcttcg gtcagttgac tcaacgatcc attgattact ccccatttac
    12961 cagccaaact gactaaatca taattattat catccataac aatcgtttct ataaaattta
    13021 gttctttcct gatttcagaa taagtggagt aatctgtata tgtcagcgga tgcttgaata
    13081 tgtccgagaa atttttggtc gaaccatacg tagaaaacga atattcaatc gttctaattt
    13141 tccattcaac gttagtttta acttgcctga gcaaattctt tttgtctgtt aaataactcg
    13201 attcgatcga ttcataggta ttctggtaaa tgaataaact aaatcctatg acaagtacgg
    13261 ctcctaataa aaacatcggt aaaaatattt tataaaatac tgtacttttc ttcattttct
    13321 tcctctcctc cttttaaaat aaaaggcttt catttttctg taaaattgat actcatctaa
    13381 ttaaatgagc attccccgct ttcaagaata gcataattaa aaataaaaat ccaagtaatt
    13441 tttaacgtgt gcaatctttt catatcaaag atctattgac ttatcgtagt tatcgtaata
    13501 tgatgaatgc gttaacaaaa agtgaaaaag taaggaaaat gatggaatgg taacgatcaa
    13561 agatatcgct aaagcagctg gcgtttctca tacgacagtt tctcgtgctt tgaataacag
    13621 cagtttaata aaaaatgaaa caaaacaaaa aatacgacaa ctagctaagg aaatgaatta
    13681 ttcgccaaac tatagtgcaa aaggtttagt gaatcaaaaa aaatatctaa tcggactatt
    13741 tttctcaagt ttgcataaag gaacttcatc aaatttccta gcagattcca ttgcaggcat
    13801 ccattcagta ttagacacaa attacagttt atctgttgaa agtatcgatg ttgttgattt
    13861 aacagaaatc aacctgcaac gctacgatgg actgatcgtt atgagtcaaa gtgatgatga
    13921 tcagcctttc atcgactatc tcaaagaaaa agaatttcct ttcgttgttg tcaatcgtca
    13981 catcaatgat ccagcggtta tcaacgtcac tgccgatgat gcggccggtg ttatgcaggc
    14041 agtagattac ggtattggat gcggacatac gaagatcgct tatattggcg gaaagaaaaa
    14101 ttttcgttcg acaaaagaac gggaaaaagg ggtattgaaa agtctaaaaa aagcgagcat
    14161 ccctgtcaat ccagcctatt ttttaacagg agattacagc ttagaaagcg gcctccaaaa
    14221 catggaatac ctattatcac tttctgacct gcctactctg gttttttgcg gcaacgacga
    14281 catagcgatt ggtgcattga gagctgtaca tttagctggc ctccgtgttc cccagaacat
    14341 ttctctgatc gggttcgatg attctccagt cgtctcctat ctcaatccgc cgcttaccac
    14401 cgtacgtaag cctttgaaag aaatgtgtca agaaggaaca aagcttttac tagatttgat
    14461 cgaaggcagg acgatcgaac aatctaaaat tgagctctcc accttgttga tccttcgtga
    14521 gtcgatcaat tacttgtaaa ctacaagtaa ttttatataa aaatgttaac gtgtgcataa
    14581 cacagaaagg aagtttatat gaaaaaactg acaaaagaat tagtgaaaaa aaacgaagca
    14641 cctgtaaaag ttcttcagtt tggtgaaggc aattttatgc gtggatttat tgattggcag
    14701 attcagcagc tgaataacca acaattgttt aaaggtaatg ttgcaatcat tcaaccttta
    14761 gaacaaggtc ttggaaatat gatgaaccag caagataatt tatatacggt cattttacaa
    14821 ggactcagca atggcgaaat cgttgatact tccgagatta tcaccgtagt agaacaaatg
    14881 atcaatcctt atgatgaatg ggatgcttat ctatctctag cagaaaatga cgacttggcc
    14941 tatatcgtct ccaatacaac agaggcagga atccaattcg tggagactga taatttaaca
    15001 gatactcccc cacagagctt cccgggcaaa ctgacagcct ttttataccg acgtttcaaa
    15061 ttaaacaaag ctggctttac catcattcct tgcgaactga ttgacagaaa cggcgaacgt
    15121 ctaaaagaaa tcattttacg ctacgcagaa aaatgggatt tagaacctgc ttttgttgag
    15181 tggttagaaa aagaaaatgt tttctgttgt agtttagttg atcgcatcgt cccaggttat
    15241 cctcgcgaag atgcaggtgt tttcaatcaa cggcataact atgaagatca attgatggtc
    15301 aaagcagaac cattcatgct ttgggtgatc gaagggccta aagaattgga gaatcaacta
    15361 ccattcaaac aggctggctt gaatgtcatc atcacagatg atatgactcc ttatcgtgaa
    15421 cgtaaggtgc atttgcttaa tggaccgcat acagcgatgg tccctcttgc tttgttggcg
    15481 gaattggaaa ctgtagaaga agtaatgaaa gatcctttat tctccgcata tgttgatcag
    15541 ttattcaatc tagaattgat tcctatgctt tctttaccta aagatgaatt agccatttat
    15601 gctgaccaaa tcaaagaacg tttcttgaat ccttttgccc atcacaaatt agaagcgatt
    15661 tcattaaata gtgtttcaaa gttcagcacc cgtctgcttc cagtgttcaa gaagtatatc
    15721 gaagaacaaa atcaggtacc accgttaatc actgtttcac tagctgcgct acttcttatg
    15781 tatcgaggcg atcaagtaaa gccacatgat gatgaaaaga cgataagcga atttactgat
    15841 gcttggtctg ataatggaac agctattcct cgattattgc aaaacgctgc gctttgggga
    15901 gaagatttgt ctcaaatacc gaatgtgaca gatacagttc aagaaatagc tgcaaagatc
    15961 gatcaaaaag gtgtccggtc agtattacaa gaaatgaaag gaacgatggt ccatgcttga
    16021 aaccctgcaa ccaacaatga aattaaatcg acgagacagt gtggtcgtcg cattaacccc
    16081 tattccaaaa caaacacaac tgacgattga tcagcaaacg atcaccactc ttgaagatat
    16141 cccgcaaggt cataagattg cattgggtga tttgaaagct ggagacaacg tgatcaaata
    16201 tggctatcca atcggtcatg tgacgacaga cgtaactgcc ggtcaatggc tgcacactca
    16261 taatgtaaaa accaatcttt ctggtgaatt ggattatcga tatgaaaaag atgtccatcc
    16321 aagtcattac ctttttgaga accgaacatt tcaaggttac ttgcgaaaaa atggaaaagt
    16381 agggattcgt aatgatttgt ttttagtacc taccgttggg tgtgtgaatg gcattgctga
    16441 actgatcgtc aaacagttca aggaaaaaca tccagatctg ggacagtttg accatattac
    16501 gattttaaag catccttatg gctgttcgca gcttggaaaa gatcatcaaa atactcgaga
    16561 aattttagcg gacgcagtaa atcatcccaa tgccggagga gttttagtat ttggattagg
    16621 ttgtgagaac aatacagttc ctgaattcaa aaaaatttta ggtgaatatg atgaagaacg
    16681 agtgaaattt ctagtggctc aagatgttta cgatgagatc gaacaaggtg tggctctatt
    16741 agaagagctt ttaacagcgg cggaaaatga ccagcgcata tcagtcccgc tctctaagtt
    16801 gaatgtcggt ttgaaatgcg gcggttctga tggtctatcg ggaataactg ctaatccttt
    16861 acttggtgca ttttctgact acttgattgc tcaaggaggc agcacgatct taacagaagt
    16921 gccagaaatg tttggcgcag aacaagtatt gatggcacga gcggaaaatg aagaagtttt
    16981 tgactcaatc gttgacttga tcaatgactt caaacagtat ttcttgtctt acggggaacc
    17041 agtttacgaa aacccatctc ctggtaataa agcaggtggg atcactacat tagaagataa
    17101 gtctttaggc tgcacgcaaa aatcaggaag ctctcctgtc gttgatgtgc tacaatatgg
    17161 cgaaaaaatc agaaaaccag ggcttagttt attgcaagct cccgggaatg atcttgtagc
    17221 tgcctctgct cttgcttcat ccgattgcca gttggtcttg tttaccacag gaagagggac
    17281 tccgtttggc tcatatgtcc caacggtcaa ggtatctacg aatacaactc tttttgaacg
    17341 caaaggtcat tggatggatt ttaatgcagg cgtcttatta gaacaaccaa tggaagtaat
    17401 cttagaatcg tttgttcaaa aaatcatcgc tgtagctagt ggagaagaaa cgaaaaatga
    17461 acaaaatgac gttcgagaaa ttgccatttt taaaaacgga gtaacacttt gattttcata
    17521 aaacagtata aaaagaaaga aggaagattt atgtttttat ctgatgattt tttattaaaa
    17581 gacgattggg cgaaaaagct ttaccattcc tacgcaaaaa acatgcccat tattgattat
    17641 cactgtcact tagatccaaa agaaatttac gaaaacaaca atttcagcaa tctgacagaa
    17701 gcttggttag gaggcgatca ttacaaatgg cgcttgatgc gagcttgcgg cgtgcctgaa
    17761 gaaaaaatca caggaaatgc ttctgatttt gaaaaatttt tagcttggtg tcaaaccgta
    17821 ccaaaaatta tcggaaatcc gctttacagt tggtcacatc ttgaattgaa gcgtttcttc
    17881 ggtattgact tgttgataaa tgaagaaaat gcagagaaaa tttggacaca agcaaatgaa
    17941 attttggcag ctcctgattt tcgccgtcgg gaaatcgcaa aaaaatcgaa tgtagaagtg
    18001 atttgtacaa cagatgatcc cgcagacgac ttgcactatc atcagttgct tgctcatgaa
    18061 gaaccagatt tgaaggttct accttcattc cgtccagata aagcactcaa tattgaaaaa
    18121 gaaggattta atgcatgggt cacactactg gaagaagcag ctgaccaaac gatcaccacc
    18181 tatcaagaac tgatagaagt cttagggcag cggatagaat acttccatca acatggatgt
    18241 cgtgtttccg accacgcttt agacatctta cgctataaag aagcggatga aacagtatta
    18301 gaagagattt ttcaaaaagc acgagctaac aaactattat cttcagatga aatcgatgct
    18361 tatcgtacag aaacactgat acatttgacc cacttctatc attctcataa ttggacgatg
    18421 cagctgcaca tccatgctta tcgtaactgt aatacacaaa tgttggaaaa attagggccc
    18481 gataccggct atgatggaat gaatgatctt tctttgacta ttcctctgca aaaattattg
    18541 aaccgtgcag aagaaacgga tcagttgcca aagacgatcc tttattcatt gaatccgaat
    18601 gactatcctg cgattttagc tttgatgggc tgtttccaaa aagaacagaa tggaaaactg
    18661 cagttaggtt ctggatggtg gtacaacgat actcgagctg gtatgcgcga tcaattgaca
    18721 caatttgctg atggtagtgc tttggggaat tttgtcggca tgctgacaga ttcaagaagt
    18781 ttcctgtcct acactagaca tgaatatttc cgtcgtgttc tttgtgaatt tttaggcgaa
    18841 atggtcatgc gaggagaagc acctgaagat gaacaacttt taggaagtct agttcaagcg
    18901 attagttata caaatgctaa aaattacttt ggcttttttg cctaattgat taatagtaac
    18961 agtaacgaat caaaaacagg atagtgaaat aacggaatcc gtttatccac gttttagaga
    19021 agaagacgga ccaaatttgc tccgtcttct tctcttgctg ttctgcttca ttgcctataa
    19081 acctttatga aagaagagat aacatgccta ctatccatat tgccggtgat tctactgcgg
    19141 cagaaaaaga tgaaaacaag cgtcccgaga caggttgggg agaaaaaatc tctgcttatt
    19201 tttcacccga gatccagatc aataaccaag caaagaatgg acgcagcacc aaatcattta
    19261 tccaggaagg aaggttagcc cgtctagctt cacagtttca gccgaatgat ttcttatttg
    19321 tacaatttgg acataacgac cagaaaatcc aagaagaaag aggtacgctt cccttcggta
    19381 gctatttaaa aaatctgcag atattttttg aatgcgcaaa aaaagcgaac gtccaaatgg
    19441 tcttactgac atctgtgacg cgacgagatt acttagaaaa cggaacattg aatccagata
    19501 tacttggaga ctatccaaaa gcgatgcgtg catttgctga gaaacaccat attcctcttt
    19561 tagatgtgtt ctcaagatca caagaattgt tccagacctt ctcaaaagaa gagacaaaaa
    19621 aattctacct gcatcttata cctgatactt gtaaaaatta cccagaagga ctaaaagaca
    19681 atacccactt tagtccagaa ggcgctgata aagttgcccg aataattgtt gaattgataa
    19741 aagaatcccg tctgcctctt gctaattatt tacaaaaagg agtttgagat gtttccatta
    19801 aatttaggca tccgtgccca cgatttagat gttcgaaatc gtgaagaaat cgttcaaaaa
    19861 gtatcacagc ttcacttgtc ccatatccaa tttgcaccac aaaaagcatt tcctgaacaa
    19921 cttaaattaa ctgacatgac acttgggagt gctgcctatt ttggtgaata cttccaaaaa
    19981 aacaatatcg aactttccat tttaggatgc tatatcaacc tatccagcaa aaatcaagaa
    20041 gttcgtgcac aagcattggc aactttcaaa cagcaaattg ctctgtgctc tgcctatcaa
    20101 gccaagatga ttgctacgga aaccggtagt gtctctgtcg gttacacaaa agaaaatttt
    20161 actgaagaag cttatcagat cgccagaaat tctatcattg aattagtcgc tcatgcagaa
    20221 aattttggcg taactgttgc tgttgaagca gggatcaatc atccaatcta taactatcag
    20281 ttagctaaac gtttaatcga tgaagtagct tcaccaaatt taaaaatcat tttagattgt
    20341 gcgaatttga tgcataaaga aaatcatgct gatcaggaaa aagtgatccg aggtgccttg
    20401 gacaatttac atggttatat tacagcgctt catctcaaag actatatcat ggaaaatgga
    20461 aagatccgca tggtacctgt tggcaagggc tgtttagatt tccgcccggt actgcattat
    20521 atcaaatatg agcgtcctct aatgtacgcc actcttgaag ctactcctga gatttatgtt
    20581 cctgaagcaa tagaacactt gcagtttatt taccaagatt gttaaggaga agattatgga
    20641 ggaacgaata cagaaaagtt atcaaaaata tcatgcaaat gcttttcaga cattgctgca
    20701 tctttatcag ggaaattatt ggcgttttgc catgtcagga ctctttttta ttatcaaaca
    20761 tcttccatct tggttaatgc cgatcgctat tgccaatgta atcaatgcag cttcatcgaa
    20821 agattcttct cagttgcagg ccatttattt caacgctttt ttgatggctt gtctgattgg
    20881 tcaaaatata ttgacgaatt attttcatgt aaaataccat agcacttcga tccgtcaagt
    20941 agaagctgga ctaagagcga cccttatcaa gaaaatccaa gaactatcta ttccttatca
    21001 aaaagaattg caatctggac gaatccagtc aaagatcatc cgtgatgtag aagctatcca
    21061 aacgttgtcc tctcaagtat ttgtcagcgg attaaatatc gctgtcaatt tattcgtagc
    21121 tttaggtatc acgctttata aaagtcctgt tgttttcatg tttttcttat tgactgttcc
    21181 tctcgccgca ttcgttactc tctttttccg taaaaggatc agacagtcca actcagactt
    21241 caggcaagaa atggaagaga cttctgcaaa agtgatcgaa gtgatcgaat tagcaccgat
    21301 tgctcgagct catgggttag aaaaacacga aacaactcgc ttaaataagc attttactca
    21361 aatctatgaa aaaggattcc acttagatat cctgcaatct gttttcggat cagtcagctg
    21421 ggctagtttt cagatatttc agctgatttg tttgattttc actgggatat tagctttaca
    21481 agggcgcatc caacccggcg atgtcgtgat gtatcagacg tatttcacga cgatcgtcaa
    21541 ttccgtttct ggaatgatca cgctgattcc tacgatttca aaaggcatgg aatctatcac
    21601 ttctattggc gaactgttga ttgctggcga tgtagaaact tatcacggta agaaaaaaat
    21661 ccaacatttg gatggaaatt ttcgttttga ggatgtttct tttctttatc cggactcact
    21721 caaaccgatt atcgatcatt tttctctaga ggtgaaagct ggagaaacaa ttgcttttgt
    21781 tgggccatct ggctctggaa aatctacact ccttaactta ttgattggat ttatccagcc
    21841 gacttcagga aaaatttttc tagatgatca gcctttggat gaactggata tgcgaagtgt
    21901 gcgaaattat ttagccgttg ttcctcaaac cactttgttg ttctccgctt cgatcaaaga
    21961 gaacatcact tatggcttga aaaacgtatc aaaagaacgt ttagatgaag tcatcgaagc
    22021 tgcgcaactt tcttcactga ttgaccagct tcctgatggt ctggatacct tagtaggcga
    22081 acatggcaac aagctgtctg gtggccaaaa gcaacgtatc tccattgcac gagcgttgat
    22141 acgtcagcca aaaatcattt tgctagatga agcaacctca gctttagata accagtcaga
    22201 gaaaaaaatc cagcaagccc tgaattacct gacagaaaca cccactactt ttattgtggc
    22261 tcatcggctt tcaacgatca aagaggcaga taaaatcgtt gtgatcgatg aaggaaaaat
    22321 cgttgaaata ggtacttacg aaaaactgtt agctcaaaaa ggctatttct accgcttgaa
    22381 aaatgaatga caaaaaatag gagctatgac aagacttttg tcgtagctcc tatttctgaa
    22441 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatagtcatc aaacatgaga
    22501 agctgttttt tgaaattgtt ccttgtaact cagagttaaa cggtaggctc acgacctctt
    22561 ggtaaatggt gttcaagaga cagcctatcg gcaataagaa gcaattttgt catcgctcaa
    22621 ggccgaacgt ctctttcaca ccctctgttg tttcataaaa tagttttatt cgctgtaaag
    22681 gtcgcgaact ggtgtcatga tgatacggtt caagtcattg atgattgtac tgaagccttg
    22741 ctctttttgc atcaagtcat tgattacttc ttctgattgg attttcacag ccatttcttg
    22801 tgctttcatt gcatcttcgt cgctgaactc ttgtccactc atttgttttt cttgtaattc
    22861 gatttgcatc gcttggaagt ctttgaacag tttgtgtgct tcttcgtttg cttgtacttt
    22921 ctcgtacgct tctttcaacg ctttgaattc tggtaattca cggatctcac gttcgatttg
    22981 attcgcgcta tcgtagatgt ttgacatgtt atgccctctt ttcttttagt agttactctc
    23041 tattgtaaca tggggttatt cgttttcaaa ggggctactc tccttgatat ttccgccaga
    23101 ggtctttagc cgcttcacca attccctgaa cgccttcttt caccatatcg ccgaagtttt
    23161 tcagcccttc ttttgcttgt tcaccatatt gattcaagtt atcttgccaa tttccagact
    23221 ctccattaga atttcctgtg tttccaactt gatctgcagc taccacttgt cctcctgtgg
    23281 cataggcatc tgctacacta aatcctgttt ttgaagtata cggaaggatg ccttctgccg
    23341 ctgatttgaa gactttcccg acaacatttc cacttgtccc ttccaaatag tgcagcttgc
    23401 tggattcttc gtaacccatc catgtcgaaa tcactacgtc tggcgtgtaa ccgatcatcc
    23461 attggtcatt tgcttttgtg gcatcgaaat tagtttctgt ggttcctgtt ttccctgcta
    23521 tcgtataacc tgcaggattc gcagcagccg ctgttccatt tgaaaatgta ccaagaagca
    23581 tgctggtcat ttcatcggct gtttcctttg atatgacctg ttttggtttt ttgtttgtgt
    23641 tatcgacgat caccgctcca gtcgaatcaa cgattttcgt gataagatgc ggcgtataca
    23701 tctgaccatc attggcaaat gcactgtaag cacccgccat gatcaatgga gatactcctt
    23761 tttccagtcc accaagggct aagccataat acttatcaga ttttgctaaa ggcaggccga
    23821 attcttctgc tttatcatat cctttttgta aaccgatctc atgcaaaagc catacagtag
    23881 gcaaattcaa actttgtgca acagcttgat acatcggtac ttctccttgg tatgtcccgt
    23941 caaaattatg cgcatcgtaa taactttgcg gttcgtcttt caagatactg gaaggcttgt
    24001 atccagcttc cagtgctggt gtatatacgc ttaatggttt gatcgttgat cctggtgaac
    24061 gacgcatctg tgtagcaaag ctgaatcctc taaagacatg atctcctctt cgtcctacta
    24121 gtgcttgtac accacctgta ttcggatcta aagctactga agcagattgg accatggcac
    24181 catctgaagc atttgctggg aataatgcac tgttttgata agtagcttct aattgctttt
    24241 gataatcctg atctaaagct gtatagattt tatagccttt attcatgaca tcttcttcat
    24301 caagaccata ctcgttaact gcttcatcga tcactgcatc aaaataataa ggataacgat
    24361 aaccagaatc tgaattatcg tatgtgtcat taagatacgc gctgatatct gtctggcttt
    24421 ctgcttgttc ttgttctttt gtgatttttc cgttttcttc cattacactt agtactgtgt
    24481 ttttccgatc attcgcattt tttgggtgat cgatcggatt ataaatacca ggacctttca
    24541 gcattcctgc caaagtagca gcctcagaca gcgtaacatt cgttgcatct actccaaaat
    24601 actttctgga agcgtcttgg acaccccata ccccgttgcc aaaataggca ttgttaagat
    24661 acatggtcaa gatttcttct ttgctgtact tcttttcgat ctcgattgcc aaaaataatt
    24721 ccttcgcttt tcgatcaaac gtttggtcta atgtcagata cgcatttttg gcaagctgct
    24781 gagtgatcgt acttcctccg ccccctgatg ttccgccaga caagaccatt cggactgctg
    24841 cacgggcaat ccctttgata tcaaacccgt gatgctcgta aaaattccta tcttctgtcg
    24901 agattactgc atcttggata tagggagaaa tcgcgtttaa ttctacatat gttccttttt
    24961 gggcgtacag tgtgccagct tcttcgtttt tcttatcata aatgactgtc gattggctta
    25021 atcctgattt taacgtttcg acatttgctt gttttgctaa aataaagagg tagatacttg
    25081 tcactaagac acagatcaaa ccaatcagca aaatcaattt gttcacatga tattttttcc
    25141 agatgcgctt tcgccatcga tggaattgtc cgagatatgg tctgatccaa cgccaaaaac
    25201 tgatcaaagc agcttttatc ttttctaaaa tgctttgaaa atccatttgc ttcttctcct
    25261 gtctatttga ttcaatcgtc ttattttacc atagtgaaag agtgttttct tcctgcttaa
    25321 gttgtagttc aatatgtttt tttacgttag tgcaagtaga gtaacataag aatgaacaga
    25381 aaaatgtaag ttttttctta aatatgtttt aaaaatggta caattagtct gcgattgcac
    25441 tgaaaggagt acacttgtga aaatcacgat cactttacct atgaaccatc ctgaaacaac
    25501 aattcgcgac ttgcttgaaa aggaatggtt agttcctaga aaggttcgac actttttgcg
    25561 tatacgtaaa aacgtttgga tcaatcaaca acctgctttg ttccatcatg aagtaaaggg
    25621 caatgaccag atcacattgt tattcgaaga aacagattat aattatcaag aagttcaatt
    25681 aggagatgcg aagcacatcc gtgttttata tgaagatgag catttgatta tagtggataa
    25741 gcctgcagga atgaagactc atccaaatga accgacagaa aacagtacct tactcaatca
    25801 tttagctgct tatctcgaca ctaaacagca acgaccttac gttgtccacc gcttggacaa
    25861 agaaacaagc ggtgccgttt tatttgcaaa aaatccattt gtcttgccga tacttggaag
    25921 aatgttggaa caaaaaatga tctatcggag atatcaagcc atcgtatggg gagaaattgc
    25981 caaagatatg accatcacca ataaaattgg acgagatcgc catgatcgga gaaaaagggt
    26041 cgtagatgat aaaaatggtc agagcgcctt gacacatgtc actgtcaaca aagtactttc
    26101 tgaacaaaca caagtctatt gtgttttgga aaccggacgt acacaccaga tcagagttca
    26161 tttagctcat aagggacatc cgatcgttgg agaccctctc taccaaaata gaacagctga
    26221 acgactgatg ctccatgcat tagaactgca catgatccat ccatttactc aagaaaaaat
    26281 agtcggtaaa gctttacctg gattatggga atagaaataa aaaagaacag agtcttatca
    26341 gcagataatg ataagactct gttctttttg tgtaatttgt ctgcttactt ttgatgttcg
    26401 tgttctgcta acagcaaact accgataatc ccactttcat tcggcagtcc ccaacgaacg
    26461 atatattcac ttacttgagg tgtttccata taaccaccca tcaattcaac aaattgctga
    26521 cggatttttt gaaggagatg atcctgattc attaccccac cgcctaaaat aatcttttca
    26581 ggtgctaacg tcaacgtata gttgatcagt gcttgagcaa tataataagc ctgaatagcc
    26641 caaacaggat gatcttgcgg cagatcttgc cctttgatcc ctgtgcgtgc ctcaatagac
    26701 ggtcctgctg ctaatccttc taaacaatct ttatgataag gacaagtacc ttcataatgg
    26761 tcatctggat ggcgtttcac ccaaatatgt cccatttcag gatgtgcgat ccctgaaaaa
    26821 atttcttcat tgatcacgac acctgcaccg attcctgttc ccacggtaag gtaaatacaa
    26881 ctattttttc cttgtgctgc tccttgcttc aattccccat atgcagcagc attgacatcc
    26941 gttgtccaag cagcaggaat attgtagcgt tccttgattg tgccaagaaa atcgaattgt
    27001 gcccaccctt ttttgggagt tgccaaaacg tatccgtaat tatcttttgt tttgtcgata
    27061 ccaatcgggc cgaatgagcc aatcccgatt cccttcaatt cataacgatc aaaaaattca
    27121 aatacctgat ccaatgtttc ttctggtgtt gtggtaggga tacttaattt ttctaaaatt
    27181 tctccaccat ctgaaaccgc acacacaaat tttgttccgc ctgcttctat tccgccatac
    27241 attcgttttc ctcctcgaat ccgattgtcc gaatctcata aggttggatt attgggacaa
    27301 ccgcttgctt cgttggttct tcaagcagat tcaatatgac agtttttcca ttgtctttag
    27361 ttatttccaa tttttctaaa tgactagaca tattgaaacc tcttactact actttatcac
    27421 taaacttgct gcgtttcaaa gctgtaattg caaacgtttc actttttatc gttaaatatt
    27481 gttgttttga aatcaatgtt ccttcatgat gtttgatttg ttgagtggaa aaaggaatct
    27541 gtgctgcgta tgcatgcaaa taagtagaga atttttcttc tggtccatgt agttcaatgc
    27601 tgtaattgaa ccgatgctca cctagacact gtgcttctgg tgttggaaaa tatccccaat
    27661 cgcctagttc tcccacactg cgaagtagcg tcactgcgat ttgtccgtct tctgttactt
    27721 catattcatt caaaccaaag tttccgactg ttacaccgta ctcttcatca tgaatattga
    27781 caaatgcttg ctgatgttgc ggattcgtgg ggttttccca acttttagac acatggtttg
    27841 ggcgcttcac cacttcaaaa atactatctg cttcgtgatg ttccacatgt aactttgtag
    27901 gaaacaagac acgtaagcga tgatctttca tctgattgtc gagagtcgtt tcaaagtcga
    27961 tttttttact atcttttctg attgtcatga ttgtttttat ttgaaggatt cgtttttctt
    28021 tggatcgttc tgcttttcga taacgaaatt ccacaaccat ctgctgttct ttttctaaac
    28081 gctcgtctgc agaaacaggg atttccattt cctgtagaat acttattttt gctatttccg
    28141 gtgtatctac taatactttt acttcagagt tttctttgtt actggatagg attggctgat
    28201 cacagaaagg ttgtttaaag atatactcat ttcctatatc gccgatatct tcaaaaatat
    28261 gcagattatt caactttcgt ttgtttttgc gatcttccat tgtaatggta ccattttttt
    28321 caatcgtcaa atgtagaggg ccgttttcga tgattcgtcc actctgatga atcattgttt
    28381 ccttttcttc tgctttcgct tccccttcta caagcgcgaa tgtttcccaa gaaaatgctg
    28441 gcatgttttc taatggtaaa gtcactttga tcattcttgc catatatggc acacggaaac
    28501 ggtctttcgg caaatcataa ccaaattgga cagtttcttc agataaaata gctggtatag
    28561 tggccccact ttttgtttct acatgatact tacgtttagg caaattttct aactcttgat
    28621 atagttgttc aggtattcct tcagcgaatc tttttctttc taattcgatg gttatttctg
    28681 cttcaccagt tttatccatt cctgcagtat ttacaatgac aaatggaaag ctgtcttttg
    28741 ggaatccggt cgtatcgatt gctgcggtca gctgttctaa ggaatcttgt gcaagatact
    28801 ttccaacttc atctgctttt tcaaaacgag gtatcatttc ccgatggaca gaatcgactg
    28861 aacaaccgca aatactgtcg tgaggatgat tttgcattaa tgtcttccaa gcataatcta
    28921 attgatcatg gggatagttt ccactaactt cataagccat cgtcgcaagt ggttctgtaa
    28981 tattttctag ctgtcgctgg accttggtat tccattgttt taagtaaacc cgagcagatg
    29041 ctgtattggc taaagtatac cagccatccg tttcttgact tgtcaattct ccttctactg
    29101 agcctaagtc ttctggcaca tctttttgaa ctgcttccag atagtcagta aaattagaat
    29161 gaataaactc gtaatctgga aacagttcat ttgcgagatg gattgctttt gatatatctt
    29221 tttgtaccgg ttgatgatca acaccattca tcatcaataa gtggttcgta gaggcatatt
    29281 gttctgcatc cgctaatttt tgcttccaaa aggcaagagc tgcttctttt tctactggaa
    29341 tctcattccc gttgctatac caattggcaa atagcagacc aaagattgct gtttgatcag
    29401 gtcctttcca ccacatctct gagtattggg aagaatagtt ttctgcttcc aaaacttgat
    29461 tatcaaagcc aattggtttt acaccacggc caaacgctgc tgctgaaata ccagcttgtt
    29521 tcattaattg aggcgtctgt cccatatttc caaatgtatc gggaaaatag cctaacatga
    29581 caggggttcc ccatttacgg ctttcttcca tgccaatcag catatttctc acgttcgatt
    29641 ccgagctgat aagaaaatca tcttgcaaga tataaaatgg tccaattctc aattttcctt
    29701 cattgattgc ttgttggatt gcctgtcttt tttctggcct tacttgaaga tagtcatcta
    29761 aaataatcgt ttgcccatcc aaatgaaaac tgttgaagga cggatcaact tcaaatagct
    29821 ctagtagatc gtctatcaat tcaatcagtc gcatgtgatg ctgttcgtaa gccatgtacc
    29881 attcacgatc ccagtggcta tgagaaatga tatatacttt ctttttcatt tacgatacct
    29941 cctacttttc tactctaata tcaaagtaat ccatgactaa ttcgcaaaac atcatatttg
    30001 cccaagaaaa ccattctctc gtataatttt caggattgtt cacatcgaat ccttcgtgca
    30061 taaggtgtgt tcctgcgtca gttgctacaa gtaaattcaa tatccgctct ttttcagatt
    30121 tgtctgacgt tgtcattcct tccatagcta atgcaatcgg ccaaacgtat ttttctggcg
    30181 tatgggagga tccaattcct tttgcgtact ttccttcata gaaatatgga ttttctttac
    30241 tcaacaatgt tcttcttgtt tgcagataat cttcctcttg ttcagaacaa tatcccaagt
    30301 aaggtgcggc gatcagattg ggcacattac tgtcatccat gactgtggcg tttcccattc
    30361 catctacttc atatgcataa atcgtttgcc ctttttgatt aaccgttcgt ccgtgattct
    30421 gaatcccctc ttggatctct ttcttcaatt tctgtgtttg ctgtacaatt tctttatcat
    30481 cgagtatcgt ttgaaaaatt tcttccacat aacctaacac gaccaccgca aacatattgg
    30541 atggtaccag atatccataa cggcaagtat catcgctagg acggaaacca gaccatgtca
    30601 ttcctgtaaa agctgttttt gcacctcgcc cgtcattgat cagcgtatct tcttttcttg
    30661 tcgtgtctcg tacaaatgta taaggagagt gttcatgatc ttgctctgtt ttgaacacgt
    30721 ccaagatttt tttgatacct tcaataaaag attgattgaa ttgatcagtt tttcctgtat
    30781 ttttgtaaag aagataggcc aactgtattg ggtagcataa agaatcaatt tcatattttc
    30841 tttcccagat ccaatcgttc atttttgtat gatctgtttg gtggccagca ccgttagctt
    30901 cttcgttgaa ggcattcgca tatggatcga tattgatata aaaaaattgc tttttgacta
    30961 acccgctgat catgtcagct aattcctcat cttcttttgc aatcactaaa taaggacgaa
    31021 cttgtgcagt agagtctctt aaccacatcg ctggaatgtc tcccgtcaat aaaaaggtgg
    31081 ttccgtcatt ttgctttttg actgttgtca acagagtatt ggcaaatgct gctgtaaaat
    31141 tctttgccca gtccgcatgg atatccccac atttttgtgt gatatcttcc ataaatttct
    31201 gcaccgattt tggtatatct ttataagcca ctaataaaac cttctttcta tttgatatat
    31261 ctttaaagat tatacttcat acagccaatt atgtctacgc tttcaaaaaa tatcttttta
    31321 tttacttttt catccattat ggtattattt gaatatgata tatcattaag aggtgtttat
    31381 atgaatcaac cattgtacca gacaatattt aatgatttgc aaacggctat tttaactggt
    31441 gaattacctg tagacagcca actaccaact gaaaaagaac tttcacgtac ctataaagta
    31501 agcagaatca cttccaaacg tgcattgact gaattagagc aattaggact tatttatcgc
    31561 gttcgcggga aaggcagttt cgttaaatcc gcttctccgg aacagattct tgcttctcct
    31621 acaaaaacac gacggattct gtttcttctt ccatatgtcg ctgatttgtc tgttggtgat
    31681 tttagcaagg gattgaaccc tgtgatgcaa gataaacaga ttgatgtcat gatgacgacg
    31741 cttgactttc tacaaaataa aacagcaaaa gagctaatca atgaatttga cggattgatt
    31801 tattatgctc ttgatacaga acaacatctt gacttacttt ttgagctttc attgaaagaa
    31861 ttccctgtcg tcatattaga taaaaaaatc tatgagttac cttttcctac cgttctttcc
    31921 gataatttcc aaggcggtgc gcttgctgcc tcttatttga tcgaacaggg acattcaaaa
    31981 atcgcttatt tgtttggtac gcagactcat ccacaatccg ttcgacagcg ttaccttggt
    32041 taccttgaag cattggacaa agctggtttg actttccaca cttcactgga tgacaaaagc
    32101 gcgacattag atgaattgct ttcttatatc actaagtatg aagtaactgc ttttgtttgt
    32161 gaaaacgatg ttgtcgctat ccagaccatg aatctcttga gacagcatgg ttatcatatc
    32221 cctgaagatt tttcaattat cggatttgat gatatccaag cagcagctct tgttgacccg
    32281 ccactgacaa ccatcgcaca agattttgaa cagttaggta agcttgcggg agatgctttg
    32341 gtcacttggt tagagacaaa agatcgacca tccgatagta aatttccagt ttcattcatc
    32401 aaaagacaat caacaaagga gaaataaaat gaatatcggt caaatagata ctagacatgc
    32461 aacagcgaat cagccaaatt tctctaatgg gaactgccag ccttatactg gcgttccttt
    32521 tgggatgaat tattttgcac cgcaaactac ggatcaaaac ggcagctggt ggtttcatcc
    32581 tgatgaccgc gtttttcaag gctaccgtct tacccatcaa ccaagtcctt ggatgggtga
    32641 tttcagtcat atgctgttta cgccgatcaa tggaaaacta caggagaaca cccttttcca
    32701 tgctcaaagt tcttaccgcc cagaagaaag tatattttgc ccgactcatc tgtcgatctg
    32761 tcaattacgt tatggcatct gttctacatt gatacctagc atgtatggcg gaatcttatc
    32821 tattgattac agcaaaaatg acagcggttt acttttatcc tttccgggac gccatcagct
    32881 ctttgttata gatccttaca cagtggaagg aaaaatcaca cattttgctg gttcagaaga
    32941 tcctgatttt tctttttatt ttatattgag atttgaatat cctttaacaa ttgatgagtt
    33001 agctgtttcc aacaaaaata gcgactcgat cccgttgtat tttgaacaga caaaaaaaca
    33061 aactatccga tttgggacat cttttatcag ctcggaacaa gcagcttata acctggaact
    33121 agaacaaaac caaagcaaag agacttacct tgctcacagt caagaacaat ggcagtccta
    33181 ttttgatcgt atacagctca cacaccatga tcaacaacaa ttgcggacgt tttatcataa
    33241 tttatatcgg gtgtttttat ttccacaaac attttatgaa attgatccta gtgggcaaaa
    33301 aatccactat gatacgacta gtcgatctat aaaacctggt gttctctata caaataatgg
    33361 gttttgggat acgtataaaa cggtttatcc tctttattct ttgattgctg tagaaaaata
    33421 cgaagaaatg ctggaaggtt ttttgaacag ctatagagaa agtggctatt tgccaaaatg
    33481 gctgtctcct gatgaacgtg gacttatgcc tgggacattg atcgatgcgg tcattgcaga
    33541 tgctgctgta aaagatatcc gacgagactt gatgcctgaa tttctaacgg caatggaaaa
    33601 aggtgctacg gttcaaagca aacatccaaa ttatggacgc caaggaacag aagattactt
    33661 gacatatggc tatgttccta atcactacca cgaatcagtc aatcacacat tagactatgc
    33721 ttatagcgat ttttgtatta gccaagttgc caaggtacta gaagaagagg aaatgacgaa
    33781 gaaatatcag cagcaatcat tgaactatca aaatatcttt gactatgagt caggatttat
    33841 gcgttcaaaa gataaagatg gcaagttcag agaaccattt tcagctaatc gttggggaca
    33901 agattatgca gaaggaagtg cttggcaaag cagctttgct gtttatcacg atttttcagg
    33961 actgatcgga aaatatggtg gcagtgagag atataaagaa aaactcattg agctgtgcaa
    34021 tcagccccca acgtttgacc caggaggata tggttttgag atccacgaaa tgagtgagat
    34081 ggccgcgata gaatttggac aattagctat ttcaaaccag ccaagttttc actatcctta
    34141 cttgttcagt tatataggac aacctgaaat ggcacagcca ctgcttaaac agttgatgac
    34201 tcagaccttc gacgattctc caatgggtta ccctggagat gaggacaatg gaagtatggc
    34261 cgcttggtat atcttcaata gtctgggatt ttatcctgtc acgcctggaa ccggtgaata
    34321 tgttatcggt atgccattaa ttgacaaggc tgttttgctt ttgtcaaatg gaaaggaatt
    34381 gacgattgaa gcaacgccta atcggccgca gcaacaattt atccataaaa tcaaacggat
    34441 ggacaaagaa tatacgaaat tatattttac ccatcaagat ttgctgaaag gcggcacgat
    34501 ctcttaccaa ttagggatca tccctcaccc taagaaatat caagcaaaag atttgccttt
    34561 ttccttatca aactataatt aacaagaggt tgtgaaaaag ctgtcctgca tcaagtaata
    34621 agaacagcca agcaaaaata gatcctcata tttttcgttt ggttggtctt attaccgcag
    34681 atgcaagctt ttgaacaccg tttattgaga ggttgtgaaa tcagcgttct gacctgagta
    34741 ttagagagac aaatggcaaa atagttcctc atattttgcc attttttgag ctaataccga
    34801 gggtcagctg attgaacacc gtttagtgga gaggtcgtga aaaagctgtt tagtttcgag
    34861 tattaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt ggcttaatac
    34921 cgagaaactg gcttttgaac tccgtttatc gagaggtcat gaaaaagctg ccttgcctca
    34981 agtaataaga acagcccaaa attagatttc tagacaattt tttataagtt atttcattac
    35041 attggctttt tcttttatga ttctccactg ttttctcggt actctttagg ggatctgccg
    35101 acgattttct tgaataattt agagaaatac gtggtgttgt tgtagccaat ctcacttgct
    35161 atctcattga tgttttgatt actgtgcaaa agcagttgtt tcgctttttc catacgtaaa
    35221 tggttcaaat aagctgaaaa actttttttc gtttcttttt tgaacagctg tcctagatac
    35281 atgacattta aatgcaggtt ttctgacact tctttgagtg taagtggttc ttgataatgc
    35341 tggtgcagga ttacgattac tttttccaca ttgtcactgt actttttctg tctctgatgc
    35401 tttttgatga atgacagaag tagctgctga agttcttgca ccgtttttgc ctgatggatt
    35461 ttttcgattc cttgtaaata ttcctcatct tccagatcaa tcaattcccg ttgaatctcc
    35521 ataaacagta aaaaactgga atatcgtata tcttccggca tcatggctgc taactgaaac
    35581 tgttcaaaaa aatcagtgat cattttttga gcgacttcca attgtttatt ttgcagcacg
    35641 cgtctgaaac ttgaaaaatc gatggtttga tcttttgttt ctgcttgtac tgcgtaaaaa
    35701 acatgctcgt ctttatctcc ataaaattga tgcaactgta gattgtcttt cgcttgttgg
    35761 aatgtttcag ggattttgtc caaagaatct gtctcttccc cgatactgat cagccattcc
    35821 tgatcaggaa agttttcttg aacaaaacaa cagaagccat caacttcatc tgcctccaac
    35881 aacaacgtga tcaatacacg gtctccgtaa tttcgttgat agtaaaagat ttgttgccgt
    35941 tcttgcaacc atctattgat aagagtatca tgagttcttg ccagttgaat caatactatc
    36001 ctatgctgct tctcacttat ttgtcctatt atttcttctt cacttatttc atccaattct
    36061 tcattcaacc attgactgaa taacagttct tgatagattt cttgttgatt tttttgttca
    36121 ttttgctgat ctaaccgtgt tttgattttc ttgaggcttt caaccaattc gaacttattc
    36181 acgggtttca tcaaataatt cacggcgcct aattgcatac ctcctttcaa ataatcgaat
    36241 tcttggtatc ctgataaaat cataaattca aaattatgac gttctttttg agccgcttca
    36301 ataaattcca gtccatttat ttctggcatt gtcacatccg ttactactaa atcaatctgt
    36361 cgctgttcaa gtacagctag tccttctttg gcattctctg ctgttgctac tatttgaaag
    36421 cctaaacttt gccaatcaat tatctttttt aacccattca aaatcatgta ttcatcatct
    36481 accaataaca cttgatacac gctcatccct ccttgaatgt aatagaaacg gtcagtccac
    36541 ctgtctcatt attctcaagc gacatataat aactgctgcc gaagctggca cgtaaacgtt
    36601 cattcacgtt ttgcagaccg atagaaccgt gaagttccac tttaggatga gacagttttt
    36661 cttcaatctg ttttagccgt ttttcagtca tcccttttcc attatctttg atgatgatcc
    36721 ttacccgttt gccttcctgc aagactttga cacttagcgc attatcgaat cgagtaaaat
    36781 caataccgtg tttaaaataa ttttcaacta atggctggat cacaaattta ggtacttcga
    36841 tcttttccaa atcaggatcg atcataaaat gataggctac tcgattagga taacgcattt
    36901 gatacaaata gacatatttt tcgcaaaagt ccagttcttc ttttaaagtg atcgttttct
    36961 cttgattcgt attgtttcga agcaaagcgg aaaacgcata cacaacatct gctaattctt
    37021 cacttccttc gctgattgca tacattcgga tatattctaa tgtattatat aaaaagtgtg
    37081 gattgatctg tgcttgaagc gcccgcatat gtgcgtcttg ctgtttgatt tctaatttgt
    37141 agatgtcttc tacaaactga ttgatgctat ccaacatttc gttgataccg attgaaagtt
    37201 ccttcagttc atattccgtg tttgttatgt cgatccgcgt ctctaaattc cctgctgctg
    37261 tttgatccat ggcctgcatg acgatagata cttgctgtga atatcgtttg aatgttttat
    37321 aaagaaagat aagcagtata ttgtctaaaa taatgatacc aatgatcagc ggggcaagat
    37381 tttttcccgt ttcgattttt acactttttt tatctagtac agctaatata cgataatcat
    37441 cagacagttc ttggtgttga acaaaattgt ttttttccag atcgtcaatc ggcaattgtg
    37501 aatttttctt cagactctct gtgatgatca tttcttgtct ttcaaacgtt tctttagcta
    37561 ttttattatc atggaatgtg tatagtcgat ttgtcgttcc agaaatcatg tataaagaga
    37621 gtccattgaa tgacgttaga ttttctaaaa tcatatctag ttctttcttt tgaaaaccaa
    37681 taaaaatact tcctgactca gctcctcctt gtgtgatagg caacttgata taaaaggcgt
    37741 ctagtaattt tggtgtcccc ttttttattt ccccaccttt gttttctcta gaagattcat
    37801 aatactccgg tagttgattc aacacgatat atatcgcact tagttcttca taatttgcgt
    37861 agagatcttt gatttgattt gggaaagaaa ctaattcatc ggtactgttt tgctgttcgt
    37921 atacatacat cagatactga tctatgggtt ttgttaagta ggtcgtcaca ttttcgatct
    37981 tttcggaatt ccctacaagc tggtttttga tgatcctact caaggttgtt ttatcattca
    38041 gcattctgct tgtgctttga acggcatcac ttgtcatgtt ttctgcctgt ttttgatttt
    38101 gttcccaagt atggatacta aaaataacgg tagcagtcat cgttacaccg atcatgataa
    38161 ccgcgtattt ccgcaacaga cgatttagta aggttccttt tgaaaataga atccgaaact
    38221 tattcataac tttccaactg tttatctaat tcttcacgcc aatgtgttgt catcgtacca
    38281 ttccaaaccg tcaaaagacc aatgacacca aacaggatca atcctttgaa ttgccaagtc
    38341 acgcctaaaa ttataccgct accaataatt atttttagaa aagacgaaaa gctggaaaaa
    38401 accgaaataa aacttagctt tagcagatta ggaacagaga tcgtatattc actatctaaa
    38461 atcatactgt attgataagt agcaaatagg cagagcaagg caaaaagtaa aataaaatca
    38521 atagctaaaa aaatgagccc tttgatttga acagataaat ataaattgta actcaataaa
    38581 gcagtaccag ataaaaataa actaaatagc agattgcctc ttaaaaaatt tcttttatat
    38641 atattccagc ctctttttat tgtgatttct ttatactcga atccgtacaa ataaaatagt
    38701 tcattgactg ttttccatgc cgcacctaat ccaaagatga tcccgccggc aaatgtatat
    38761 atccagaaat acaaggttag ttttataatg acccagatcc ggataaataa ctcttccaaa
    38821 accttcccta acatatcttc tcctccaaac attaagttta tactttctca ttatacaaaa
    38881 aaaggttggg caatcagcac cttgacctga ttattagaga aacaaacgga aaaatagttc
    38941 ctcatatttt gtcgttttgt gagcgaatac cgagggttag ctgattgaac accgtttacc
    39001 aagagtttgt gaaatagccg cttagtttcg agtagtaaga aaaattttgt gaatatggct
    39061 ttccacattt ttacatatat ttagcttaat accgagaaac tggcttttga acaccgttta
    39121 ttcggaacaa gaggttgtga cacgtctttt gtcacaaccc ctttgtcttt attcattatt
    39181 attcttgtcg gaactcatca tattgttttt gcatttcttt caatacctta tcatagccag
    39241 ccttgtccaa agcgtctttc aatttcggta atgtttcatc tggatcaaca gtccctgtat
    39301 tcaatccatc taaatattga ttcatgacat tagaaatatt acttaattct gttttgacac
    39361 tgtcagtgtt gaagctgaat cctaaaatcg gtgaagtttc tgcatcagca atcgattgat
    39421 cacgtttggc aatcatgtca tccgtaatag actcttgtgt atatagaatc ttgttgtttc
    39481 ctgtattcca agcagacata tgtgtatttg gctgataacc atctagcaat ttgattttat
    39541 catctgaacc atcgactttt tcccatgctt ctccctcgat cccccaaaca agcccattca
    39601 ataattctgg atcactgtta agcagcccta gaagttctac tgatttttct ttattttttg
    39661 aagtatttga tacgacaaaa tttgccattt gtgcttgagc tgtagatttt agtggaacag
    39721 taattgcttt tgagacaagt tcttgttttg ctgcatttgt caaaattgta tcgccataat
    39781 catacggtcc ttgtgtttct ccacgcatta gccaagtatt tccttctagt ggataatcct
    39841 tattgctcgt tgcagcgtca cttggaatga gtccttgttt gtaccagcta tgcattgtac
    39901 gtaacagtcc gatatacgtt tcattatcgt attggttgat gattttcgaa tcatctccat
    39961 ccagatcaat agcaaatggt aacgtattcc ctaaaggata atcaaagttc ccttggactt
    40021 tgaagccttg tccaatggca aaagctgccg tgtttggttc tttttcatgg aattccttca
    40081 agactttttc tgcatcagcg taagattgga ttccatcaat tgaaagatcg tatttatcta
    40141 gtaaagcttt gttaaaagta agtacttgtt gagcaaagac atttccattg actgggaaag
    40201 catataattt tccattgacc agatttcctt taatatatgc ttcatctaaa tcattataag
    40261 ctttttcagc gtacttaggc gctaattcag tcaaatcagc aaaggctcct ttttgggcat
    40321 tggaaacata gttgttcgca aaggcgatat cataattttc cccggaagaa atgatgacgt
    40381 tcattttctt ttcgtaatcg ccccagccaa tgtaattgat attgactttt acaccgatct
    40441 ctttttcgat tcgtttgttt gctacttcca tcaattgatc gaaattatct ggtttatctc
    40501 cgatttgata catctgcaaa gtgtccgaat tgctagcttc tgcctggtct ttagaaccag
    40561 aacctccgca tgctgctaat gttcccaaca acaacgctga aacacctgta aaggctagtt
    40621 tcttccataa cttcattttt cttcctccta attttatgat ttatattttt gtttctttat
    40681 tcttttactc cgccgattgt tagacctttg acaaaatgct tctggaaaaa tggatagctg
    40741 atcgcaatag gcaacgtcga gatgactacg atcgccattc tggcagattc tcctggaact
    40801 gaggctaatc cgcctgtcaa ttggcttcct gctcctgaat tttgtgtcag atattgaata
    40861 ttactttgga ttttcatcaa aagatactgt agtggaacta gcttatcatc ttggatgtat
    40921 agtaaagcat tgaaccagtc attccagtaa cccaatgcgg caaataaact gattgtcgca
    40981 atacctggaa cagctaacgg taaaacgatc tgaacaaata ttcgcagttc gctagctcca
    41041 tcgatccgtg ccgattcaat aatcgaatca gagaccgaac gtttaaagaa tgtgcgcatc
    41101 acaataatat tgaagggact gacagccatt ggcaagatca atgcccaaat cgtatctttt
    41161 aactgaagaa gatttgtcat aacaagataa ttggccacca tccctggtga gaacaacatc
    41221 gtaatcaaac aaaaaacggt aaaaaaacga cggaatggaa agtttgaccg tgaaatcgca
    41281 taagcatata aagaagtagc cgtgctattg atcagtgtac ctaaaacagt aacaagtaca
    41341 gtgacgaaaa gcgcttggaa aattttatcc tgcatctggt cgaacaagta cgtataacca
    41401 aatatactga acttttctgg ccaaaaactg tacccgtgag tcgacaatat ggactcatcg
    41461 gtaaaagaga tggcgataac aaatataaat ggcaaaatac aggaaatagc aaagagtgca
    41521 atcattatat tagagaaaaa attagccgtc ttattgaaag agcggacttg gactctctcc
    41581 actttctttt ttttcatttt tttcctcctt agaataatgc agaatcgcta tcaaaacgtc
    41641 gggcgatcgt atttgtgatc atcaacaaga caaagccgac aactgattgg taaagtcctg
    41701 cagcagccgt cataccaaaa tctcctgtgg atgtcaggcc attgtagata tacgtatcca
    41761 aaacttgagt cacatcatat aatgcaccgg aattcctagg aatgttataa aacaggccaa
    41821 aatcagctcg gaaaatgttt cctacggcta aaatcgttaa aatcgtcatt agtggaataa
    41881 gttgtggtat cgtcacatgt ttgatctgct gccatttgct agctccatca accatagcag
    41941 cttcataata agtcggatcg attcccataa cagatgcaaa gtaaataatg ctgttataac
    42001 caatcccttt ccatgtacca atgaacacca agataaacgg ccaatatttc ggttcattat
    42061 accaattgac agcttctcct ccatgtccag tgatccactg attgaatatt cctttgtcag
    42121 gacttaaaaa agcgtaaaca aagtaactga taatgaccca agatagaaaa taaggtagta
    42181 aggacatcgt ttgataaact ttaacgagtc gtttatttct caactcgctc ataatgattg
    42241 caaatacgac tgagatcagc agattcaaaa ggataaatgc tacattatat aaaattgtat
    42301 ttttagtgat gagaaatgct tgatccgaag aaaataaaaa tttgaaattt tcccaaccca
    42361 cccaaggact ttccctcaag ctagccaaga acccatctgc tgaaatatga aaatttttga
    42421 atgctacgac attcgccatt acaggtatgt aaaagaaaaa aatcatccaa agcatacccg
    42481 gtacagccat caggacaagt gctttataac gccatagatt cataaaaaaa tgattgaacc
    42541 cctttcgttt tttcatctaa aaaccccttt cgattcattc gcaacgtaat tatagcgctt
    42601 tcaataatga gaataaataa acaaagtata gggaaaatga aacaaagtat aggagaggtt
    42661 gtgaaatcag cgttttggcc tgagtattag agaaaaaaac gggaaaatag ttcctcatat
    42721 tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt atcagaggtt
    42781 gtgaattcag cgttttggcc tgagtattag ggagatcgtg tccttactat ctaactctga
    42841 gtcacaagga acaatgggga ctgtaacaaa agtcttgtca cagtccctta ttccgaacaa
    42901 acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa aagccatccg
    42961 aaagtcagac ggacaagtct gcttttcaga tggcgctaca actactctgc ttattctttt
    43021 agctatccat tcaattattt ttctctcact tgttctagct cttctttggt catgggtcga
    43081 tattgccctt tattcaaatc tttaggcagc caaaattttc ccatacggat tctttttaaa
    43141 tataagactt cttttcctac tgctttgacc attcgcttaa cttggtgaaa ctttccttca
    43201 tgcaaaatca gacggataat tgaagtttct gtctcttgat ctacttgatc aatttggagt
    43261 tttgccggca atgtctgctc atcgccatca ataatcagtc cttctgcaaa taaacggata
    43321 tcttcttctg tcattatacc gctaacctca gctagatatt ctttctccac atgtttttta
    43381 ggtgatagta actgatgggc aaacaccccg tcattcgtta aaatcaagag gccctctgtg
    43441 tctttatcta atcggccgac tggaaaaaga tcttcccgat aatcctggtc ttccaataaa
    43501 tcgattacgg tttgatcatg attatcctga gtagcagaaa tgactcctgc aggtttatgc
    43561 aaaatgtagt aaaaatcttt ttgatacgta atgatttctc ctaaataagt aaccgcatct
    43621 tcgaattcat ttacttgcgc tttatcgctt ttcaccactt gtccattgac ttcgatcgtt
    43681 ccttttttga ttaatccttt gacttctttg cggctgccaa gtcccatatc tgctaaaaac
    43741 ttatctaaac gcatgcgttt cccccatttc aaaagagcat caatctagct cttctgattc
    43801 acttatttta tttttaattt atgtcgtaaa cgcgctattc ctggtcctag tagtctttct
    43861 gccaagcgga ttttcaaagc taaataacca tacactaaag cccctatacc tgctactaca
    43921 agaatcaaca atagagactg gaatttgcta tcttcgttta aaaaagcacc aaaaatcatc
    43981 tttgtgatcc acgaagccaa aagcataatc aaggtaagaa taaagatcag caatgtcctg
    44041 cgccagacaa aacttggttt aaagcgggct acttcatgga ttctctttaa gatcaaggta
    44101 caagacacag caaatccgat cattgtcgcc aataaagggc cgtatacttc aaataaacga
    44161 atggctgggt attgaagaag gagtttcact agaaacccaa taaccaaata attaattgcc
    44221 gccttgtttt caaacattcc ttgcagcatg ttggaaacta gcatatatag tcctaggaag
    44281 agtcctacaa aacttgcttg gatcagtaca ttacttccta gagaatccgg tgtataaaat
    44341 aatgtattta gcggataggc aagtaacata acaccaaatg tcgccggaaa cataaaaaat
    44401 gaaaacaact gtaaatttcc gcttgtcaat tttgccagac ctactcgatc tttcaacgta
    44461 acagcttctg taatcaacgg taacccagta gacgcgatcg atgttgccaa tgcaataact
    44521 accatcgtca acttatctgg attggcacta aaaatcgaaa aaagatccag taactgtgca
    44581 ttggaatact cggtggatgc agacatgatc ttcataaaag tgaactgatc gactaatttg
    44641 aagatcgtta cccctgaacc cacaataata aacggaatcg cttctttaac ggtatctaac
    44701 aacaattcct tcgttgcaat atttattttg ttttcgctgt agtgaataaa cgccgatgta
    44761 tacgcttgat gtttcttcaa aaaatacaaa aggacaagaa tacttgctag cataccgata
    44821 aatgccgcga aagttgattg cgtgaccgcc gtcacatagt caccatccat gactttcatg
    44881 atgatgaatg tagccaatag catataaaaa atacgtgcca cctgttctac aatctgcgac
    44941 agcgcataag gcatcatttc ttgattgcct tgcaaatacc ctcgtatcac actcatacaa
    45001 ggaaaaacca atacagcgat actcaaagaa cgcatcgttg gaatcagttc ttctccgcca
    45061 ccagaagcat gagctagcca aggagaggcg atgtacatga acaaagcaaa caacaggcct
    45121 aacccgccca tcatctgtaa tgcacggata aataatcgtc gtgaagtgcc atattcattc
    45181 aatgaattat agcgtgcagt ttgttttgca atcgcagcag gtattcctgc tgtagagatc
    45241 agtagaaaca atgcatatat attgtatccc atattgaata atccattggc agccttagca
    45301 ttttcgccca tccaagcata ccagggaatg atatagacag cgcctagcaa tcttgaaatg
    45361 atattactgg cggtcatcca agcagagcct cgtgccattt tttcttgcgg tgttaattga
    45421 tctaatgctg gttttctttg attatccatc ggtcagattc caactttcta caaaacatta
    45481 ctatcatttt aattggtaaa acagaaactg gcaatcgatt ttatataact tttataagaa
    45541 caattgtttt tttgaattcg gtttcgtttt tttggtaaag taaagaaata tacagccttt
    45601 tatggctaac atttatagga gatatcaatg acacaagaaa taaaaaaact acccgttata
    45661 tttttaggtc ttttctttat cacagttgga ttgaattggt tcttgctccc tcacgatata
    45721 gcttccgccg gagtgggcgc catcggacat ttagttgaaa cggccttttc tatccaacgc
    45781 tcagttactg tttggagcat caatcttatg atgctgcttc tagccgctgt cctattagga
    45841 aaatcagtat tttttaagac tgtagcggga agtctgcttt ttcctgttgt tttagagatt
    45901 gttccaaaag tggaaatgat ctcctcacat ttcttttcac tgatttcagg aagtcttttg
    45961 ttcagtcttg gggtttacac attgtacaca gtcggggcct caaatggcgg tataacgata
    46021 ccgccgatta ttttccacaa attttttcgg cttccaatgc cgcaaggact tttacttacc
    46081 aatagtctta tcgtcttttt gaattattat gtttttggat tgttggagac actattcgtt
    46141 ctcctatcta ttacagtcag ttcattgttt atgaaaatac tcatccgctt gtcccctgtg
    46201 tcaagaaaat ttgaataaag ctggattttg tcatagcgga aacaaacgat atattaaaaa
    46261 gagtgtgcta taataaacca agaaattgaa taaagcgagg gatcaatcaa tgagcttctc
    46321 tttagaagac atccgtcatt tactattaaa agaaaatcta ttaaaagaat ttgtttcaac
    46381 aagaggctgg catcttgatg taccagaaaa agctgaattt cttcagttaa gttatgattc
    46441 aagaaaagct gatgcttcta ctttgttttt ctgtaaagga atgaatttca aagaagaata
    46501 tttggattca gcaatcgaac agggtattca atattatgta tcagaacaac catacgaaaa
    46561 ttcagcagta ggaatcatcg taacggatat ccgtaaagca atggcgcttc ttgctatggc
    46621 tttttacgac tatccgcaaa ataaattgaa agtgatcgga ttcactggaa caaaaggtaa
    46681 aacaactgct gcgtacttta caaaagcaat cttggatcac acaacaaata aaaaaacagc
    46741 attgctttct actatgaata caacgttaga tggcaaaaca tatttcaaat cccatttgac
    46801 aacgcctgag tcattagatt tatatcgtat gatgtcagaa gcagttgaaa atggtatgac
    46861 tcatctagtg atggaagttt cttcacaagc ttacaaaaca caacgcgtat acggcttgac
    46921 tttagatgtc ggaatcttct tgaatatctc gcctgaccat atcagtccga tcgaacatcc
    46981 gacatttgat gactatttct attgcaagcg acaattgatc ttgaattcaa aaacagttgt
    47041 tttaaatcat gaaagtgact attttgattt acttagggaa acagccgatc tattccagat
    47101 tccaacgatc acttatggtc gttcggaaga aagcaattat caagtgatcc gttcagataa
    47161 aggtactcac ggttttactt tatcttctca tgaagatcgc ttagctatct gcgatactgc
    47221 ttatgatatt ttgctggctg gaggattcaa ccaagaaaat gcagccagtg cgatcattgc
    47281 cgcagcacta acaggtgcct ccagtcaaga tgcacaagac ggattaaaag aagctcgtgt
    47341 accaggccga atggatcaat tgatccaatc caatggagcg catgtctatg tggactatgc
    47401 acataattat ctaagcttga aaacattgct gaaatttgcc aaaaatgaac atccagatgg
    47461 ccgtgtaatt gttgtattag gcagcccagg aaacaaagca atctcccgtc gacatgattt
    47521 tggaaaagtg ctatcagaaa cagctgacat cgcctttttg acggcagatg acccagcatt
    47581 tgaagatccg aaaaaaatcg cggaagaaat caatgaagca attactaatc cagatttgat
    47641 cgttcattat gaaatggatc gtcctgaagc gattcgacgc gctttagcag aaagcgctcc
    47701 gcaagattca gtcgttattg cgggaaaagg cgtggatccc tatcaaaaaa tcaatggtgt
    47761 agatgaacct tatgaagggg attatgcaat cgtgaagcgg ttgattgaag aataatggta
    47821 tttatatact agtggtttcc catttctaag aaaaaaaggg actgtgattt tttgtcacag
    47881 ttcctttttt cttagaaatc attttaactt ttcactagct aattctgctg ccttttctct
    47941 ttcttcattt aataatttat tatcatagaa tttgataaat gggaaccaga taatgaatgc
    48001 gatgattgca caaatgactg ctaacacagc agctttccaa tcaccaccac taccaacaaa
    48061 tgctccgatc ccgactggtg aaggccacgg catctgtgca agcatcggtt tgacgatttc
    48121 cagccgaatg gcaaaataag cgagtgtagc agaagccatc ggagctaaga aaaacggaac
    48181 cgctaaatag ggattgtaga cgattggcat cccgaaaatg attggttcat tgatattgaa
    48241 taatcctggt acaagtgagg cttttcctaa cactttcagc tgatcagatt tcgcgaggaa
    48301 tgcaataaag atgatcaatc ctaaagtcgc acctgatcca ccaacggtta cataagaatt
    48361 gttgaattct cctgccaacg gataatttgc gccttgtgcg ttagctgcca tattagctaa
    48421 tacgatcggg gtcaagaaag aagtgatgat attcgctcca tggatcccta cgatccacaa
    48481 tgcatggatc aagaaataaa tcaccatgac accaagccat gtattcgtca actgcgtaac
    48541 aaacccaaat gggatagcaa tcattttgaa gatatccgtt cctagtgcaa ctaataatcc
    48601 attgatgatc atcaccacaa acgcaatgac aaatgttggg atcagagcag tgaaagaacg
    48661 agaaactcct ggcggaacaa catctggcat ttttatgacc caatttctct tgacacacat
    48721 acaatacaat tcgaccgcaa taatcgacat aataatagct gtaaagattc ctgaagttcc
    48781 taaacgttcg acaaaagagc ccatcctaat accatttact acagtttctg tctcattgat
    48841 agaagcaatc aagctgattt taccatcagt catgatcaat tcagggatac acataaagaa
    48901 tgcaaaaaca gatagtaatg cccccgtgag cggattaaca tttaattttt gttcatctcg
    48961 ttcaattgac gttaattcat aagcaaacac aatggcaaag taaatagata aaatccccat
    49021 cgtcattgta ttgacgatca tgtacaagtc actaactttg aaaaaagttg cattaaaaaa
    49081 tccttctaaa aatgggaacg gtaatggcaa aatatttaaa accaaaaaca tcgagcctac
    49141 aatcgtgaaa ggaatagaag ccatccccgc tgccatgaca gcccgaacga ttttaagttg
    49201 cgcaattttc cccataggtc ccatcaggta tttctgcaaa aaatcaaaca ttagttattc
    49261 tcctttccta aatataacgc tttttcatat tgtttaatac ggatatactg ttggtcttgg
    49321 ttgagtgaaa ttttctttaa acgatactgt ataaatgcac tggcaaaaat accgcttaca
    49381 acaagaatga aaacaaataa ctgtcctcca ttgttatttg ccataaaagg atacaagaat
    49441 gtaaatcctg aggtaaacaa cactggaatc actaataaat taaagacact ctggaacaaa
    49501 aaataccact ttgtccatgg aacgttcgtt tggtgttttc gataaagctt tacttgttct
    49561 cctactgcag gcaaaatagc taaaatcaga atcatcggta aactacttcc taaactttta
    49621 gctgatagaa gcagaatcat ccattgcatg ttcatgaaaa agaaaaaggc agtcaggtaa
    49681 cgtacgatta gataacgatt gaaatacatg tttttcaaac taagttgctt tttttctaat
    49741 tcgattttcc tttttactgt atcctgcaca atgatccttc ctttctattt atttttttga
    49801 tacaaataat tcatttcctg tgcaacttcc aataatgtca tcgtcgtcat cagatgatct
    49861 tgtgcatgga ccatgataat ttccatttct atttttgtcc cgctggcata ttctttcaga
    49921 agttgagttt gtgactgatg cgcatgaaga atctcttcat ttgcttgtcg caacttctct
    49981 tctacttctt caaacatctc tttacgcatc gagcgaaatg cttcatgtat cagtgtccgt
    50041 gcatttccgc tatggaaaat gatatcaaaa gaagtcactt gtagtgtttc actgtttatt
    50101 ttttcagcca tctgttttcc tccttattcg atcagtttga taaatttact gctgaaattc
    50161 tcaaaaatag gttcagctaa gatttcctgc tgtactttta gctggtcaac taacctaacg
    50221 attgctttag tcacgaccgt aattccttca ttttcgacat aagaaggcga aactaaaaaa
    50281 acaaaatgaa tttcttgata ttgatcccac tgcatcccag aaggaatcaa tgcaacggct
    50341 acctttgttt cgattccaac tggaacagcg ggatgtggga ctgccactgt atcactgaat
    50401 atgatttgtc ccatattttc acgttgctgg atctgctttc gcatttctct tatatatcct
    50461 tcttcttcat ttatgctcag cttttgcagc aaatcatgaa gaatttcttc ctttttcggt
    50521 tcgtacgtat aagtcttgaa aaatttttca gacaactgtt cggtgtaaat ctgctttttt
    50581 tgctgtagag aaaatgctgg tatcggtcta tctgaggaat gatagggtat agattctcca
    50641 actacctttc gtattttacg aacatcatct tcattcaaaa aaatactgac atgtaaaact
    50701 ggaattttga aaaacattgt cgaaagatca atagaagaaa taatcaggtc gattcctttt
    50761 aaagttgttt cattgatctc ataatagcct tttacactta ccactgtaat atttttaccg
    50821 aattcactga ctacgcgatt ctttaatagt tgggcactgc cgtaacccgt tgcacagata
    50881 atcaatgcat gtagtttatg cgcagctctt tctttttcca atgccgccat gagatgcaaa
    50941 gccaaatagg cccattcatc ttcattgatg gtatagtttt tcaaagccgg catgttgctg
    51001 aaatgccttt ttactgcgtc aaagactttg ggattttctt tctttatctc ctcagtcaat
    51061 gggttctcaa tgcgaatacc tcggctcaat cgaatcatca tcggtgacaa atgatcgagt
    51121 agtccattga aaagctggag atcctctact aatgaaaaat gaaattcctg agacaatctt
    51181 ttcagaactt tcgctaattc ctttgatagt tcctgatcat tttgattttc tttgtgattc
    51241 gacttagcca tgagatgtaa tgtcaaataa gcaatttctt cttcgggaaa cgatacacct
    51301 gcgattacct caatacgttg aacgatccgc tgcgccactt gatactctac tcgcttagaa
    51361 acttcttgtt cgatgcctaa ttccttgatt tcaaaccctt ctcggagccg tttgatcccc
    51421 aaagccaagt gtaaagtcag attctgtatg ataaaatccg aaacttttaa cttagcttct
    51481 cttgtttcat cgagaatgat aatgactagc tcttcaaaac tgatgtctgg gaaaaaacgg
    51541 ctttgcccta aatattcttt caatgaattt ccataatggt ttccaaaaaa agtatccatg
    51601 ataaaatgcc gtcgatttct ttcttcccct tcgatccatg tcccttgacc atgcttagaa
    51661 acgattttta gtgaatatgg aatcaatcgc tccttgattt cttgcatgtc ctttgtcaaa
    51721 ctggaacgac tgatataaag ctcttctgcc aaatcatcca taaaaagaat ggcatcttcc
    51781 aaaagcaatt ggttcaatat atacttttgt cgatcttcgg actcataaaa tccttgcggt
    51841 tctctagcat atccaggtac tatttcttgc cgactcaaaa acaagtcaaa tgtcagtctt
    51901 tgtaaaatat gcagttggta gccataacct ggtttagcaa tgatcttacc tccgtttttt
    51961 tctactaact ctttgatccg ctgcaagtaa ttacggattg tccgatcaga taataacaat
    52021 tcagaagcta aacgttgact ggtgacatac tccttcttgt gatcaatcaa atatctcaac
    52081 agcaattttt ccttctgttt cattatcatc cactcctatt ccatcatatg ttctcttcga
    52141 tcacctctgc taacttctgg atgcctgtag ggattgggac atacgcttgg aaagggatac
    52201 taacaacagg ctttccaaca gattctccta attttttgaa tttatccaaa aacatcgtcg
    52261 tttgcgggct gatcagaaat aaatcaaaat cactattctt aatcatatta tcgccttctg
    52321 tagctgaaac tgcatctacg agtacctctt ctcttttttg cttaaagaaa tccgttgtct
    52381 ttgaggccat catcgaggat gacattcctg ccgcacaaat aatcaatgct tttttcataa
    52441 atcaatcact ccttttattt ttataaacca agcatatctt gtaagcgttt tctattcaaa
    52501 atatatattt ccgtagcatt gcggaaaaaa tgtgtatcat atcttgaata ataatttctg
    52561 gcaccgtgat atactagtga tagaattttt tcaggagggg atttagtgag tatcacagtg
    52621 aatattttgt atacaagtac aaatggacaa gccagaaaat ttgcagaaga aatggtcgaa
    52681 agaaacattg tcaaagcgat tcgtgcagaa gaaggaaacg agaaatatga ctattacttt
    52741 ccactagaag atccagaatc tcttttactg atcgaccgtt ggaaagatga agaagcaatc
    52801 gaaaagcatc acaaatccga aatgatggct caaataaacg aactccgtaa aaagtataaa
    52861 ttaaaaatga aagtcgaacg tttcactgat ttaccgtaaa aagatacaca tggaatgata
    52921 gcaatttatc atccatgtgt atcttcactt tttccttatt ttttattcga tctcatctaa
    52981 tgattcaaac cgtgtttttc tcttattttt cggataagta tttcagtcac cccaatatac
    53041 atgattccta aacatacccc aaggatattc tcaaaaatgc ggatcgttac tgctcctgga
    53101 accccgaata aactggcagt gattgccaaa gcaccaaaag aattaaaaac tgttttatat
    53161 ctataggtag aacaaacacc taaagctaga ccacctaata taccgaccca gttaaaagga
    53221 ataaattggc tgataccaat aaataaaaca catccaatca atgagccaat gattcggtct
    53281 actgctcgtt ctttgaaacc aatcgacatc aatccatagc tagatacaat agaagaaaat
    53341 gcaaaccctg cccacatcaa tcgctgaaaa gggacatact ctcctgcaac gatcaaaaga
    53401 ctaatgccaa atgcatagat gagcatccaa attttttcct gcgaatacat gcctttcaaa
    53461 aaaatctctt taaacaagct tttgtctttg ttcttttctc gatgttttct gtaaaggaga
    53521 actgaaaacc aacaaaaaaa caaaaataat aagctacttg tttgtgtaaa gaaattcttg
    53581 ttcaacgaat ctttaggtaa cgaataaaca ataaagagat atgaaaagcc gtacaaagaa
    53641 gcattcccca ttttgggatc actagcggtt gccattaata tgctgcttag taaaataaaa
    53701 tgaatcaaca tgcccataac tgaccatttg atcagctgga catacggtgc tactaatagt
    53761 gagaacatga cgattgctaa acctatgatc gaatgggaaa tcttataacc aaagtcaaca
    53821 aaacgaatac tcatcaacat acagaataaa accacagcgt atggtttatg cttcgattca
    53881 aataatgttg taattataat gataaaacaa atggcaaaca atacgatgaa taatgatcgt
    53941 aaaaacaatg aacaaatata catgcacttt tcttttttag aagctgcttc tcgtattttt
    54001 tgttttaaaa taaacggatc tagttgtaga gactgataaa aatccacctg ttgtcgtcct
    54061 ttctttttac ttttattttt tacagtcatc tatgacaggt tgtaaatact atggaacatc
    54121 atatggcgtt gtcattttct tgtcaactat ttttcctatt catgttttta taaaaaaaca
    54181 tatcgttttc ttgtatgatt aaatcaccac aaaataaaca actgagagaa cgatatgctt
    54241 tatgaatggt tctatcaaaa aaattctgag atcaatagac aaaaatttga tgattacaga
    54301 ggtctcttac gaaaccttac agaagaaaaa aacattgatc gttgctgcag ttctcttccc
    54361 tgctccatgt gtctgtagaa attgtggttc tgctgtggta gatggaaata ggaaattaat
    54421 ggtggtgaaa aacaagaaga aggaaaccat tgtccacttt gaataatata atcatatgcc
    54481 aatggttata cgcctaaaaa agcagcgcta tacctgtaaa aactgtcgaa gccattggaa
    54541 cgctcaaagt tattttatcc gtccaaggca ctcgatttca aatcatgtca gacataaaat
    54601 cacttcttta ctgactgaaa aagtgtcgtt attttttatt tcgaaaagtt gttaggtatc
    54661 tttgacaacc gttattcgca cattgaaaga gttgagaagc tatttaccaa agtcttccaa
    54721 aagaattctg tcttgagtgt tgagggttgt gaatttcgtt ctcacgtttc cgttgaggat
    54781 aaactgagtt ttatttccgc ggatggcgag acagaaaaat taatcgagaa aatttaccta
    54841 cacaaaaaat tcctcgatta acaaactatt tccttagctg cactaatcct aaagaaatag
    54901 aattcttagt tacagacatg aacgccacct actttcagct caccaaacat gttctaccaa
    54961 atgcgaaatt ggtgattgat cgattacttg gtttttccac gcctttcaag gaggcgtaac
    55021 ctttattcac gagttggttg aagctttccg agacaaaaga cccgacttgt ttttctcctt
    55081 attgacaaag agccattaaa actagagaaa aaattacaac tgatcgctga ttatttgtag
    55141 aaagaaaatc aaagtattac ccaataaact tgagtcatta ggcaaaaaac actgcgaaac
    55201 tcaagaatga ttttcgcagt gtttttgtat attttttatt aactagtatt actacaaagg
    55261 tttatttttc acttctcaac gcagcacaaa attgtcttta cttatttact ggttgggttc
    55321 aaccttctct actccgatac taatatttgt ttgtataccc cttctaaaat cggtagtttc
    55381 cattcaattg atgggtacta gttgatttta aaaactatgc ttattcaatt ttttatttta
    55441 gctcatatat ttcgtttctt tctaaatttt atactgatga aaacaagtag cacacccaat
    55501 attgacaata taaactggct aatgatggtt tctccagttt ttggaagatg cttatttcca
    55561 ctgcttgttg gattgtttcc tcttatatct gagctatcag caacttttct attctctttt
    55621 gttaaaaaga tcgccacttc ttgttctttt tcgatcttga aagttactgg tgtcgcatcc
    55681 aattcatacc ccgttggcgc ttgagtttct accagctgat atgctcctgg tgctaagcca
    55741 tctatcgcca gttttccatc tgctcctgtg gttagacctg actgcagaac ggttccattg
    55801 gcatcttgta actcaaaaac agctccttgc aggacttctc cgctttggtc atcgatcttt
    55861 gtcaacacga caccgcctgg tgtaagtcga ttttctttcg tcaattctac caccgctgtc
    55921 tgacttcttt cgatctcaaa ctcgattggt gtcgcatcca attcataccc cgttggcgct
    55981 tgggtttcta ccagctgata cactcctggt gctaagccgt caatcgccag ttttccatct
    56041 gctcctgtgg ttagacctga ctgcagaacg gttccattgg catcttgtaa ctcaaaaaca
    56101 gctccttgca ggacttcccc gctttggtca tcgatctttg tcaacacgac accgcctggt
    56161 gtaagtcggt ttgctttcgt caattctacc accgctgtct gacttctttc gatctcaaac
    56221 tcgatcggtg tcgcatctaa ttcatacccc
//
LOCUS       DAZKOR010000008        55998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000008 DAZKOR010000000
VERSION     DAZKOR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.008"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..540
                     /locus_tag="ACG8ZM_000404"
     CDS             22..540
                     /locus_tag="ACG8ZM_000404"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0491397.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            668..1351
                     /locus_tag="ACG8ZM_000405"
     CDS             668..1351
                     /locus_tag="ACG8ZM_000405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0491398.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1660..1733
                     /locus_tag="ACG8ZM_000406"
     tRNA            1660..1733
                     /locus_tag="ACG8ZM_000406"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1694..1696,aa:Arg,seq:tct)
     gene            1892..2251
                     /locus_tag="ACG8ZM_000407"
     CDS             1892..2251
                     /locus_tag="ACG8ZM_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0491399.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2374..4005
                     /locus_tag="ACG8ZM_000408"
     CDS             2374..4005
                     /locus_tag="ACG8ZM_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0491400.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4067..4708)
                     /locus_tag="ACG8ZM_000409"
     CDS             complement(4067..4708)
                     /locus_tag="ACG8ZM_000409"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0491401.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4894..4970
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4990..5298
                     /gene="rplU"
                     /locus_tag="ACG8ZM_000410"
     CDS             4990..5298
                     /gene="rplU"
                     /locus_tag="ACG8ZM_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0491402.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5325..5669
                     /locus_tag="ACG8ZM_000411"
     CDS             5325..5669
                     /locus_tag="ACG8ZM_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0491403.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5682..5975
                     /gene="rpmA"
                     /locus_tag="ACG8ZM_000412"
     CDS             5682..5975
                     /gene="rpmA"
                     /locus_tag="ACG8ZM_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0491404.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6409..9216
                     /locus_tag="ACG8ZM_000413"
     CDS             6409..9216
                     /locus_tag="ACG8ZM_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0491405.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9407..9898
                     /locus_tag="ACG8ZM_000414"
     CDS             9407..9898
                     /locus_tag="ACG8ZM_000414"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0491406.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10001..10990
                     /locus_tag="ACG8ZM_000415"
     CDS             10001..10990
                     /locus_tag="ACG8ZM_000415"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0491407.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11023..11826
                     /locus_tag="ACG8ZM_000416"
     CDS             11023..11826
                     /locus_tag="ACG8ZM_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0491408.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11847..12761
                     /locus_tag="ACG8ZM_000417"
     CDS             11847..12761
                     /locus_tag="ACG8ZM_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0491409.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12844..13218
                     /locus_tag="ACG8ZM_000418"
     CDS             12844..13218
                     /locus_tag="ACG8ZM_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0491410.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13357..13602
                     /locus_tag="ACG8ZM_000419"
     CDS             13357..13602
                     /locus_tag="ACG8ZM_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0491411.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13774..14838
                     /locus_tag="ACG8ZM_000420"
     CDS             13774..14838
                     /locus_tag="ACG8ZM_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0491412.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15084..15509
                     /locus_tag="ACG8ZM_000421"
     CDS             15084..15509
                     /locus_tag="ACG8ZM_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0491413.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15502..15951
                     /gene="nusB"
                     /locus_tag="ACG8ZM_000422"
     CDS             15502..15951
                     /gene="nusB"
                     /locus_tag="ACG8ZM_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0491414.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16048..16902
                     /locus_tag="ACG8ZM_000423"
     CDS             16048..16902
                     /locus_tag="ACG8ZM_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0491415.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16903..18240
                     /gene="xseA"
                     /locus_tag="ACG8ZM_000424"
     CDS             16903..18240
                     /gene="xseA"
                     /locus_tag="ACG8ZM_000424"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0491416.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18245..18469
                     /locus_tag="ACG8ZM_000425"
     CDS             18245..18469
                     /locus_tag="ACG8ZM_000425"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0491417.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18471..19361
                     /locus_tag="ACG8ZM_000426"
     CDS             18471..19361
                     /locus_tag="ACG8ZM_000426"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0491418.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19370..20188
                     /locus_tag="ACG8ZM_000427"
     CDS             19370..20188
                     /locus_tag="ACG8ZM_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0491419.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20295..20744
                     /locus_tag="ACG8ZM_000428"
     CDS             20295..20744
                     /locus_tag="ACG8ZM_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0491420.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20762..22444
                     /gene="recN"
                     /locus_tag="ACG8ZM_000429"
     CDS             20762..22444
                     /gene="recN"
                     /locus_tag="ACG8ZM_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0491421.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22499..22615)
                     /locus_tag="ACG8ZM_000430"
     CDS             complement(22499..22615)
                     /locus_tag="ACG8ZM_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0491422.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22688..23644)
                     /locus_tag="ACG8ZM_000431"
     CDS             complement(22688..23644)
                     /locus_tag="ACG8ZM_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0491423.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23815..24183
                     /locus_tag="ACG8ZM_000432"
     CDS             23815..24183
                     /locus_tag="ACG8ZM_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0491424.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24353..24784
                     /gene="mraZ"
                     /locus_tag="ACG8ZM_000433"
     CDS             24353..24784
                     /gene="mraZ"
                     /locus_tag="ACG8ZM_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0491425.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24805..25764
                     /gene="rsmH"
                     /locus_tag="ACG8ZM_000434"
     CDS             24805..25764
                     /gene="rsmH"
                     /locus_tag="ACG8ZM_000434"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0491426.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25769..26170
                     /gene="ftsL"
                     /locus_tag="ACG8ZM_000435"
     CDS             25769..26170
                     /gene="ftsL"
                     /locus_tag="ACG8ZM_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0491427.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26171..28363
                     /locus_tag="ACG8ZM_000436"
     CDS             26171..28363
                     /locus_tag="ACG8ZM_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0491428.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28389..29351
                     /gene="mraY"
                     /locus_tag="ACG8ZM_000437"
     CDS             28389..29351
                     /gene="mraY"
                     /locus_tag="ACG8ZM_000437"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0491429.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29365..30726
                     /gene="murD"
                     /locus_tag="ACG8ZM_000438"
     CDS             29365..30726
                     /gene="murD"
                     /locus_tag="ACG8ZM_000438"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0491430.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30752..31840
                     /gene="murG"
                     /locus_tag="ACG8ZM_000439"
     CDS             30752..31840
                     /gene="murG"
                     /locus_tag="ACG8ZM_000439"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0491431.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31855..33075
                     /locus_tag="ACG8ZM_000440"
     CDS             31855..33075
                     /locus_tag="ACG8ZM_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0491432.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33231..34556
                     /gene="ftsA"
                     /locus_tag="ACG8ZM_000441"
     CDS             33231..34556
                     /gene="ftsA"
                     /locus_tag="ACG8ZM_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0491433.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34579..35820
                     /gene="ftsZ"
                     /locus_tag="ACG8ZM_000442"
     CDS             34579..35820
                     /gene="ftsZ"
                     /locus_tag="ACG8ZM_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0491434.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35837..36514
                     /locus_tag="ACG8ZM_000443"
     CDS             35837..36514
                     /locus_tag="ACG8ZM_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0491435.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36528..37127
                     /locus_tag="ACG8ZM_000444"
     CDS             36528..37127
                     /locus_tag="ACG8ZM_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0491436.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37208..37429
                     /locus_tag="ACG8ZM_000445"
     CDS             37208..37429
                     /locus_tag="ACG8ZM_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0491437.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37478..38260
                     /locus_tag="ACG8ZM_000446"
     CDS             37478..38260
                     /locus_tag="ACG8ZM_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0491438.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38281..38988
                     /locus_tag="ACG8ZM_000447"
     CDS             38281..38988
                     /locus_tag="ACG8ZM_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0491439.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39250..42048
                     /gene="ileS"
                     /locus_tag="ACG8ZM_000448"
     CDS             39250..42048
                     /gene="ileS"
                     /locus_tag="ACG8ZM_000448"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0491440.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42097..43623)
                     /gene="zwf"
                     /locus_tag="ACG8ZM_000449"
     CDS             complement(42097..43623)
                     /gene="zwf"
                     /locus_tag="ACG8ZM_000449"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0491441.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43638..44285)
                     /locus_tag="ACG8ZM_000450"
     CDS             complement(43638..44285)
                     /locus_tag="ACG8ZM_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0491442.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44469..44798)
                     /locus_tag="ACG8ZM_000451"
     CDS             complement(44469..44798)
                     /locus_tag="ACG8ZM_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0491443.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44975..45703
                     /gene="sfsA"
                     /locus_tag="ACG8ZM_000452"
     CDS             44975..45703
                     /gene="sfsA"
                     /locus_tag="ACG8ZM_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0491444.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45719..46732
                     /locus_tag="ACG8ZM_000453"
     CDS             45719..46732
                     /locus_tag="ACG8ZM_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0491445.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46732..48009
                     /locus_tag="ACG8ZM_000454"
     CDS             46732..48009
                     /locus_tag="ACG8ZM_000454"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0491446.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48072..50774
                     /locus_tag="ACG8ZM_000455"
     CDS             48072..50774
                     /locus_tag="ACG8ZM_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0491447.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50926..51243
                     /locus_tag="ACG8ZM_000456"
     CDS             50926..51243
                     /locus_tag="ACG8ZM_000456"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0491448.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51273..51593
                     /locus_tag="ACG8ZM_000457"
     CDS             51273..51593
                     /locus_tag="ACG8ZM_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0491449.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51701..53161
                     /locus_tag="ACG8ZM_000458"
     CDS             51701..53161
                     /locus_tag="ACG8ZM_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0491450.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53229..53450
                     /locus_tag="ACG8ZM_000459"
     CDS             53229..53450
                     /locus_tag="ACG8ZM_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491451.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53481..53663
                     /locus_tag="ACG8ZM_000460"
     CDS             53481..53663
                     /locus_tag="ACG8ZM_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0491452.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53663..54076
                     /locus_tag="ACG8ZM_000461"
     CDS             53663..54076
                     /locus_tag="ACG8ZM_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0491453.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54199..55380
                     /locus_tag="ACG8ZM_000462"
     CDS             54199..55380
                     /locus_tag="ACG8ZM_000462"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0491454.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55451..55615)
                     /locus_tag="ACG8ZM_000463"
     CDS             complement(55451..55615)
                     /locus_tag="ACG8ZM_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491455.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55769..55842
                     /locus_tag="ACG8ZM_000464"
     tRNA            55769..55842
                     /locus_tag="ACG8ZM_000464"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55803..55805,aa:Arg,seq:cct)
     gene            complement(55911..>55998)
                     /locus_tag="ACG8ZM_000465"
     CDS             complement(55911..>55998)
                     /locus_tag="ACG8ZM_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0491456.1"
                     /translation="YLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        18504 a         9463 c        12072 g        15959 t
ORIGIN      
        1 taaagaaaga agttgaaaac gatacctgat tacaaaaaaa taacttttat aagttctata
       61 acgttgatta tatttttaat tcttattctt ctcgtcgtct tggttccccg aatttttggt
      121 ttgactcctt atattattga ggacaaagaa atgagtccat attatgcaca aggaaatctg
      181 atatatgtac gaccgaaaaa tacaaatgag attttagtcg gagatattat tacatattat
      241 gaaaattcag gaaaaaaaat ctcgacgcgt cgcgtaatag ccgttgaaaa caaccacgaa
      301 gatttttata caaaagctga tacaaagact tatattgagc ctggagtagt aaaaaagaga
      361 aatattatcg gtagtcccat ctttcagatt ccatatattg gactagtcct ttcaaaaacg
      421 gcatttgcat ggattcaggg gttattcttt acgattgctt tttgcctaac aggaatcaca
      481 gcatggaata cctttgtaga gttgaaaaag aagcggagac gaacacagaa atttttgtaa
      541 gaatgttaaa aacataaatg tattccagag atccaacgac tcttctgaac atgaaacacg
      601 agaaaacact catgcagctt gtagatttag tcagttgttc gtataatgtg tgaagaggag
      661 gagtagtatg cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac tattggattt
      721 tatgcgtacc cagcaaaaaa aattaccggg tgagctaggc aaactggaag aagaagctca
      781 tgtagcagaa gtaccagtta ttccccatga aacagttgta tttttgaaat ttttacttgg
      841 acagcttcag cctgaacgaa tcttggagat cggagcagct atcggttttt catcaagtgt
      901 catggccacg acaatcggag aaaacgctca tgttacgaca attgatcgtt tcgatgtcat
      961 gatcgagaaa gctaaaaaaa actatgaacg tctaggactg acagataaag tgactttgct
     1021 tgaaggtcaa gcagcagata tcttgccgga gttgtctggt ccgtatgact ttatttttat
     1081 ggatagtgca aaatcaaaat atattgaatt tttacctgaa tgcttacgtt tgttaagaaa
     1141 aggtggcgtt ttgatggtag atgacatctt ccaaggagga acgatcttgc tcccagatga
     1201 agagattccc cgtggcaaac gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt
     1261 gatggatcat cccgatttga catcgacaat cttgccatta ggtgatggtg tgatcttaat
     1321 gacaaaggaa tcagaaacaa tcgagttgtg atacatgatt atggcagcct gtagataatc
     1381 gattgaatcg agggctcaat tcgaacttga tgtaaaagtt tctatatttg taagtcaatt
     1441 aaaggataca ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt
     1501 caattgataa ccttagaaca gctgaaggag aatgtctcgt tttaaaagat tgatttagta
     1561 gttgggccaa aataattcat tatttgctaa ttaaataact atttcgtatt gacagaagca
     1621 atttaattat ctataattat tgatgttccg ctgattcttg tcatagtagc tcagctggat
     1681 agagcatccg ccttctaagc ggacggtcgg gggttcgaat ccctcctgtg acgctaaaaa
     1741 accgttagaa actgaggttt ctaacggttt ttctttgttt tttgtgccaa tattgtttgt
     1801 tttcaaaggc aaataaaata aaaaaacatc cttatcgagt acaattataa ggagcaaaaa
     1861 aataaaactc atcagaaaag aggaagaaga tatgacgata ctatctagaa ttttagtgac
     1921 tctagttgca attgaatttt tttatatcat gtatatcgaa acggtgagaa ctgattctga
     1981 cacgacaagt agggttttta aaatgagtaa agaagagtta agcagaaaat ctgttcaaac
     2041 attgtttaag aaccaaggag tgtacaatgg actattagga gtgggactac tttatggagc
     2101 ttatctttct tccgcatcta aagaaatcac aagtatgcta ttaatatcta ttttttttgt
     2161 tgcattatat ggcagtttga ctagcgatcg ttggatttta ctcaaacaag gtgggctagc
     2221 tctgttggca ttgctttcta ttttcttata aatatagaaa aaataagaat ttatctaaca
     2281 ggaaactttg tgaaaaatat tactttatct catgaaatat gatattctaa tgaatggtat
     2341 tttagaaaat ttcatgaaaa aggagttaga taggtgttca aaaattatgt ttcctcacta
     2401 aaaaaagaat tttcaggtta tgatctttca agatttcaga aagatctttt ggctgggatc
     2461 acagtagctg ctgtagcatt gcctttagcc ttggcttttg gggtatcaag cggagctgat
     2521 gcggccgcag gtttgattac ggctatcatt gccggtatcg ttattggcgg tttatcaggc
     2581 ggtttttatc aaatatcagg tccgacaggt gcgatggcag ctatcttgat gtctattgcg
     2641 gcaacaaagg gaatgcaagg tgtattgctt gctacttttt tagcgggtct atttttactt
     2701 gcagcaggac ttttccgatt aggaacactg acttctttta tcccagcacc cgtaattacg
     2761 gggtttactt caggtattgc tatcattatt gcattaggtc agatcgataa tttatttggt
     2821 gttcactcgg aaggtacaaa cgttattgaa aaactaagta gttatcaaac gataggattt
     2881 ccgataaaca cagcctcttt gatcatggga gggctagtca ttttagggat gtttatctat
     2941 ccaaaaaaat gggcgaaacg aatgcctagt tcgctgctgg ctatcatttt ggcaacagca
     3001 gttatgctat tgttccattt accagtagca actgtgggta agattccaca aacattgatc
     3061 agtagtaatc gtttgaatat gggagatttt agcttttctg ccttgcaaca agtggcagtt
     3121 ccagctatta gcattgcttt attaggaatg attgaaagct tgctatgtgg tgcttcggct
     3181 ggccgaatgg caaataagcc cttagatagc aatcaagaat taatggcgca aggaattgga
     3241 aatctgttgc ttcctttctt tggcgggatt cctgctactg cggccatcgc ccgtacaagc
     3301 gtggccatca aatctggtgc acaaactaga ttagctggta tgatacatgc agttgtctta
     3361 ctgctctcca tgcttgtttt tgcgccagtg atgtcacaga ttccaatgcc agcacttgca
     3421 ggtgttttga ttgtgacggc ttggcgtatg aatgaatggg aaacgatcaa ggagctattt
     3481 actaataaat attggtcagc agttctattg tttatcctta cgatgatttg tacagttatc
     3541 tttgatttaa gtattgctat cgtgatcggg gtgatcggcg gctgtgtatt ttttgttgtc
     3601 aaaagcgctg ctatcacgat ttcattagaa acgatcgatt ggcaaagaat ggggctgcca
     3661 gaaacggaaa agctgaataa ctggacagta gtgtatatta gtggtccact gttttttatg
     3721 tcttcagaaa aactcaaaaa taaattggaa gaactaaatc atcacgatgg tgtcattttc
     3781 tccatgcgag gcgtaccaag tattgatttg acggcacgtg cagtttttga tgaatttcaa
     3841 gaaaaagcag agcaaagagg acaaacgatt atctatactt ccttgcagcc agaagtagaa
     3901 aaacaacttt ctcacttgtg ggaacataca ttcacgcatc aacatcaaac agttgctcat
     3961 gcgttgacag ctttacatgc gaaatatact gcaattagcc attaaaacaa tgaaaaagaa
     4021 cagacaagac tgtaaactga agattttatg gtcttgtctg ttctttttaa tattgctgta
     4081 aaagtgaagc tagtacttgc cagtcttgat ggataacagg tacaagggag cgattgtaga
     4141 aaatagctgc tggatgaaaa agtggaagaa ttcgatagtt tttttctgac cattcgtagg
     4201 catccttttg ttctgttaat tgtaagacag gccctgtata gagttgacca tgatttttgg
     4261 taaccgtata ttctttgcca agtaaacgtt gaagaccaat gtttccaagt gtcgcaatta
     4321 attttggctg gaccttcatc agttcgtaat ccaatattgg tgcatgcgca aatacttcgg
     4381 aacgagtagg tgtccgattt ggataaacag tttctgactg ttgtgtccgt ttattgatcc
     4441 gatgagtcac gcgatacggg cggctcctta cagcactggt gatatacacc tcttctcgtg
     4501 ttaagccagt tgaagaaagt gcctgcatca attcttttcc agcttgaccg ctaaaaggaa
     4561 catgcgtatc aatctctgtt ttccctgggg cttctcccac gagcatcagt ttaggatgaa
     4621 gaggtccttg cccagcgata aatccttcta caggacggcc gttcattctt aaagaagctt
     4681 cttgtatcag ttcttttggg tattccattt atgctcctcc tttttctttt attataagaa
     4741 taaaagaaac acaatgcgac tattttattt ggtagaactg tgaggataga aaagcagaag
     4801 tattgcttga atttttgttt tactttttga ttttaaattt agttgactaa cggttgctgt
     4861 tctggtaagc tagtactgtt gtaattgtgg actccacagc tacaaccgct cagaacaagt
     4921 aataagcaca gcatatgctg tcacttcgga tggcgagtct aagtatatca gaaggaggtg
     4981 ctttatagca tgtacgcaat tattaaaact ggtggtaaac aagtaaaagt tgaagtggga
     5041 caagcaatct atgttgaaaa acttgacgtt gaagcaggag aaaaagttgt ttttgacgaa
     5101 gttatcttag taggcggaga atctacaaaa gtaggcgctc caactgttgc aggtgcaact
     5161 gttgaaggta ctgttgaaaa acacggcaaa caaaagaaag tcgttacttt caaatacaaa
     5221 cctaaaaaac attcacaccg taaacaaggt catcgtcaac catatacaaa agtggttatc
     5281 gacgcgatca acgcttaata gcgtttgaga aagaaggcct atatatgatc aaaggaacat
     5341 ttaagcgaaa tgacgcaggt cagatcgttt cattcacttt gaccgggcat gctgatgctg
     5401 gtccttatgg aagtgatatc gtttgtgcgg gtgtttctgc gttagcgatc agcactgtca
     5461 acgggattgc ctcattagcc ggttttgagc cgattgttga aatgaatgaa gaagaaggcg
     5521 gttatcttta taccgaagtc acttcaggaa tgacacaaga acaaaacaat attgctgaga
     5581 ttctattaga aaatctttta ttaggattac aatcgataga agctgaaaac tcagaatata
     5641 tccaaattaa aacaattaat gataaatagg aggtgcagac catgttgtta tctatgaatc
     5701 tacaattatt tgcccacaaa aaaggtggcg gttctacatc taacggccgt gactcagaat
     5761 caaaacgtct aggtgctaaa cgtgctgatg gacaaacagt tacaggtgga tcaattcttt
     5821 accgtcaacg cggtacaaaa atctatccag gtgtaaacgt gggtatcggt ggagacgaca
     5881 ctttatttgc aaaagttgat ggcgtagtac gtttcgaacg taaaggccgt aacaaaaaac
     5941 aagtgtctgt ttatccagta gctcaagaag cttaattgaa tcttatgatt aaagaggttg
     6001 tgacaagact tttgtcacag ccttttttgt ttataaaaat aaaagttgta gtggaggcta
     6061 cgaagtttac tttgtctttt tacacttaat atttttttgc caatttggaa tcaggatgtt
     6121 ttgataacta tcgttttcaa ttgatggaag ctatattgtt tgctatcagt gaaataatta
     6181 tatggtaaac agtgttcttt ttcttgtttc cattataaaa atcgtatcgg gatttttcgt
     6241 tcttttaaat tgaacatttc tatctatccg cgtgttatca acaaaacaaa atcaggtaaa
     6301 tagttgacga aacagaaata aatacatagt attatgtttt gaagcctaag tacaatagtc
     6361 tatagaaaag gagcgatacc attggcaaaa cgcattgacc gaatttatat gtatgtcaaa
     6421 gaaaaaaccg ctcatttaac aaaggcagaa tatgatcaag gagtaacaac acaggaaatt
     6481 gctgaagtgt tagggattca gcgaacaaat agcagtaaag atctgaatca gctagtgcgc
     6541 gaaggaaaac tgataaagac agacggacga ccagtacgtt acatatatca aacactcgtt
     6601 acgcacgtaa agccagttac aaaacatgta gtcagttaca aagaaacgcc ggtctctgaa
     6661 gaaaaaaata cactccaaat agatactaag gatatctttg caaaaatcat tggagctaac
     6721 ggcagcatga aaaattcagt ggaacaggca aaagctgcga ttttgtatcc gccaagagga
     6781 ttaaattgtt tgattacagg acctaccgga tcaggaaaga cttattttgc acacgcgatg
     6841 tatcattttg caaaagcgca tcatgtaata gatgaggaaa cggaactgat tgttttcaat
     6901 tgtgctgatt atgccaataa ccctgagctt ttgatgagtc atttatttgg gtacgcaaaa
     6961 ggtgctttta caggcgcaga tgaagaaaaa atggggatta tcgatcaagc ggatggcggt
     7021 atgctgtttt tggatgagat tcaccggttg ccgcctgaag gtcaagaaat gatcttttac
     7081 tttatggatc atggaactta cagtcgtctt ggggaaacaa cgaaatccca tgaagcaaat
     7141 gtacggatcg taggtgcgac cacagaagac ccaggttctt ctctattaga aacttttgtt
     7201 cggcgtatcc caatcaatat caaacttccg gcttttgaaa aacgtcctgc aaatgagaaa
     7261 gtcgatttag taaaaatcat gattgcccac gaagcaaacc gcacgcagcg aaagatttct
     7321 ttgacagaag atgtagcaaa agcattgatc ggcagtgtga cctatggaaa tataggacaa
     7381 ttgaagtcaa atatccagtt agtatgtgca cgaggatttc taaatcatat gcaatcaccc
     7441 gaaatctcga tcacaatcga tgatttgaca gaagggattc gtagcggatt gattcaatta
     7501 gcaagcaacc gcagtgcaat ggcagagctt tctaaaatat tggagccaaa gataactgta
     7561 gcaccaaatg atacgatcat gaaaatccaa tcggactcct atgagttacc ttataatcta
     7621 tatgatatca tcggagataa agcagcttta ctgaagtcgg atggtttgga tcaagaagca
     7681 atcaatcatt tcatttctac agatatcaac atccatctga agtctttcta taaagaccat
     7741 ggtttttctt ttaatgcaga cagcaaatta gcagaatttg tagatccgaa aatcatagaa
     7801 gtgaccaatc agatttatgt aatggtcaaa agatctttac catatgagtt tcagcaaaac
     7861 tttatttatg ctatgagctt gcatatcagt tcttttttga aaaaaatcaa cttaggagaa
     7921 gaacggcata cgaatgataa catccgtgag atggcttttg attttccaga tgaatatgct
     7981 gttgcgaaag aagtgcgtag ttatatcgaa acgtattttc aagtggagat tccagaaagt
     8041 gaagactatt acttgaccgt tctactcgtc tcgttacggg caaatcaagc aagcggcaga
     8101 atcggtgtag tcgttgcagc tcacggaaac agcacagcaa gcagtatggt ccaagttgtc
     8161 caacagttgt tagatgcaga gaacgtacga gcagtagaca tgcctttgga tatggatccg
     8221 aaaacagcat tggaccgaat tgaacgcaat gtacaagaag tagatgaagg aagcggggtc
     8281 atactcttag tagatatggg gtcccttgcg tcgtttaatt cgcaaattca aagagacact
     8341 ggaattccag tacgtactgt tgatatggta acaacttctc tagttttgga aactgtacga
     8401 aaggtctcag tattagggac agatctagat atgttgcatg aatcattgaa aaattttcga
     8461 ggttacgcag aaatatcttc tgtagaatct gttcaggaag caaaacctat tcataagaaa
     8521 aaagctgttt tggctatctg cgcatctggc gaaggtacag cacaaagaat caaagaattg
     8581 atcgaacgag cggtaaacaa gcgtcaagaa acagaattag aagttctagc tttgtcgatt
     8641 gtggaagtga aagaagaact gccagttatc caaaaagaat atcagatcat agctactact
     8701 gggattactg atttgaatat ccaagcccca tttattcctt tagaacgttt tattgatcaa
     8761 aatatcgaag tcattttgga tcagttgctg atggaatcag aattggagga aactgaattc
     8821 atctccctag atgaagagtc agctaaaaat acgtgtgttg agtttatctc tgataacttc
     8881 atctttatta atggtagtaa gttaatcgat ccaatgtggc aattttctac acaaatctct
     8941 cagacgacgg gaatcggcga tgaagaatat ggattcaaaa tcaacctagt gatgcacaca
     9001 gcaggaatga tcgaacgtat tattcggaat gaaccattga ctgtggaaga aaacgaattg
     9061 acaaatacaa cgaatgatcc tctttatagc caattggcag cctcagttgt tctgttggaa
     9121 gatcagataa aagtaaaagt gccgatagaa gaaatgtatt atctgctacg tctagtccat
     9181 aatcagttag ataaaaaaga atacactgtg ccatgaatac acaaaaacga tacactttta
     9241 tagtgtatcg tttttgtgta ttggttaaga atcctttaaa ttcaattagt gtatcgtaaa
     9301 aaagttggca cgctttttgc ttatagtagt gtgtgctagg aggtggttgt tcacacagaa
     9361 atgatagggg tttctgtgaa gtagtgagga agaaggagtt ttgcagatgg atattcgatt
     9421 ggcacgtata gatgatcgtt taatccacgg gcaagtcgca acagtttggt cgaagcaaac
     9481 aggtgttgaa cgtatcattg tgatcagcga tgtagtcgct caagataaat tgagaaagtt
     9541 tctactaaaa gaagctgcac cgccggggat cacagcaaat gtaataaccg tgcagaaaat
     9601 gattcaaatc tatcatagtg gattattatc acgagcaaaa gtgatgcttt tattcaccaa
     9661 tccccaagat gttgaagcgg ttgtccgctg cggtgtacag ttgaagtcgt tgaatatcgg
     9721 cggtatgagt tttactgaag gcaaatcaat gattaccaat tttgtttcag taaatcaaca
     9781 agatattgat tcctttcgtt acttgaacag tcaagaagtg gagttagaga ttcgaaaagt
     9841 acctgctgac aggaaaatcc ttttgatgga actactagcg aaaatcaaaa aggtgtagca
     9901 agaatggttg ttggaaaagg tcaaataatt ctgttgtaat ggttagcaat tattaatatt
     9961 tttgaatttg ttacaacaaa agtaaatagg aggtataaat atggtaggga ttatcctagc
    10021 aagtcatgga caatttgctg aaggaatctt acaatccggt tcaatgatct ttggcgaaca
    10081 agaaaacgtc aaagccgtga ttttgaaacc aagtgaaggt ccagatgact tgagagccaa
    10141 attggaagaa gcggttgctt cttttgataa tcaagacgaa gtgttattct tagttgattt
    10201 atggggcgga acaccgttca atcaatcaaa cacattattc gaagagcaca aagataaatg
    10261 ggcaatcgtc agcggtttga atttaccaat gttgattgaa gcctacgcat cacgcttttc
    10321 aatggaatct gctcatgaaa ttgcagcaca catcattgaa acagcaaaag acggtgtaaa
    10381 agtaaaaccg gaagaattag aaccggcaga agcaccgaaa gcagcagttg aagacgcaca
    10441 acctaaagga gcacttccag aaggaactgt ggtcggagac ggaaaaatca aatatgtatt
    10501 agcacgtgtt gactcacgtc tgttacatgg acaagttgcg acagcttgga caaaagcagt
    10561 gcaaccaaac cgtattatcg ttgtttcaga tgcagtatca aaggatgatc tgcgtaagag
    10621 actaatcgaa caagctgctc ctccaggtgt aaaagcaaat gttattccta ttagcaaaat
    10681 gatcgaagta gcaaaagacc ctcgttttgg aaataccaaa gcattgcttt tatttgaaaa
    10741 tcctgaagac gtattgacag ctgttgaagg tggcgtagac atcaaagaat taaatgttgg
    10801 gtcaatggcg cattcggtcg gtaaagtcgt tgtaagtaaa gtattgtcta tgggacaaga
    10861 agacgttgaa gcgtttgaga agcttgaaca aaaaggtgtg aaattcgatg tacgtaaagt
    10921 acctaatgat tcacgcgata acatggatga tattttgaaa aaagcaaaag ctgaattagc
    10981 aaaagcgtaa taaataatat taaaatttat aggaggctta tcatgtctat tatttcaata
    11041 attttagtcg tattagttgc ctttctagct ggtatggaag gtattcttga tgaatttcaa
    11101 tttcatcaac cactagtggc atgtacattg atcggtttag ttaccggtaa cctcgaagct
    11161 ggtatcgtac ttggcggtac gcttcagatg atcgctttag gttgggcaaa tatcggtgcc
    11221 gctgtagcac cagatgctgc attagcatca gtagcatcag caattatttt agttttaggt
    11281 ggacaaggtg ttaaaggtgt tccttctgcg atcgcgattg ccgttccttt ggctgttgca
    11341 gggttgttct taacaatgat cgtacgtaca atcgctgttc caatcgttca tttgatggat
    11401 gcggctgctg aagaaggaaa catccgtaag gttgaaatgt ggcacatcat tgctgtatgt
    11461 ttacaagggg ttcgtatcgc ggtcccagca gcagcgttgc tatttatccc agcacaaact
    11521 gtgcaatcat tccttgaatc aatgccagca tggttaacag atggtatggc tatcggtggg
    11581 ggaatggtcg tagctgtagg ttacgctttg gtaatcaaca tgatggctac aaaagaagta
    11641 tggccattct tcgttattgg tttcgttgta gctgcaattt ctcaattaac attgattgct
    11701 ctaggtgctt taggtgttgc attagctctt atttacttga acctttctaa aatgggtggt
    11761 tcatcaaata atggaggcgg aagcaactct ggtgaccctt taggcgacat tttgaatgac
    11821 tattaatctt gaaggaggaa aataaaatgg cagaagaaaa aatcaaatta tcaaaaaaag
    11881 atcgtttagc tgttgcgtgg cgctctacct tcattcaagg ttcatggaac tacgaacgta
    11941 tgcaaaacgg tggctgggca ttttctatga ttccagcaat caaaaaatta tacaaaacaa
    12001 aagaagaacg ttctgcagca ttaaaacgtc acttggaatt ctttaataca cacccataca
    12061 ttgcttctcc aattttaggg gttacattgg ctctggaaga agaacgtgcg aatggcgcgc
    12121 ctgttgatga tgtagcgatt caaggggtta aagtaggtat gatgggtcct ttagctggtg
    12181 ttggtgaccc agtgttctgg tttacagtac gtccaatgct aggtgcacta ggtgcttcat
    12241 tagctatggg aggaaatatc cttggaccaa tcatcttctt cttaggttgg aacattattc
    12301 gttgggcatt catgtggtat acacaagaat ttggctacaa agctggttct aaaattactg
    12361 atgacctttc tggcggattg ttacaagacg taacaaaagg tgcttcaatt ttagggatgt
    12421 tcgtattagc tgcccttgta caaagatggg tatcaatcaa attcttacca gttgtatcaa
    12481 ctgtaaaatt agataaaggt gcttacatcg aatgggataa attgccttca ggtggtgaag
    12541 gaatcaagag tgcatttgaa caagttaata atggcttagc cctttctcct gaaaaggtaa
    12601 caacattaca agacaactta gaccaattga ttcctggttt agctgcatta ggattaacat
    12661 tgttatgtat gtggttgctt aagaaaaagg tttctccaat cgttatcatc cttggtttat
    12721 tcgtagtcgg tgttgttgga cacgtcatcg gacttttata atccataaaa atatagctcg
    12781 ggcgttgtcc cgagcttttt ttatatagta tactgttcat aaacaaatat ctggagggag
    12841 aaaatggttc aatcaatcaa tacaaaagta gacttggtaa ttgatgctac ttcgtttacg
    12901 ggtctgacag attacgggca aatcatgata ggtaataatg gttttgagtt ttaccatgcg
    12961 cgagatagac gcaaatatat ccaaattcca tgggaagaag tggattatgt actagcatca
    13021 gtcatgctaa agggaaaatg gattcctcga tatgccattc aaacgaaaaa aaatggtaca
    13081 tacacatttt cttccaaaga acctaaaaaa gtgcttcgtg cgatcagaga atatgtagat
    13141 gaagatagaa tggtacaatc attgagtttc tttgatgtag tgaaacgtgg tattcgtagt
    13201 atgtttaaac gcaagtaaaa aataaagaaa acttctaaga ggaggttttc tttatttttt
    13261 tactttgcag aagaaaatat gaaatagcaa aaccaattat ttgtcccctt taaatcccat
    13321 atgctaaaat tgattagcaa agaaggaggt tatattatga gttggttatg gacattgatt
    13381 gttggagccg tgatcggagc aattgcaggt gcgatcacca gtaaaggaaa ttcgatgggc
    13441 tgtgtaacga atatcattgc tggactagtc ggttcaagta ttggacaagc gattttcggc
    13501 gaatggggac caaaagctgc aggtatggca cttattccat ctattttagg cgcagtgatt
    13561 ttggtagcag ttgtctcatt tttctttgga aaacgatcat aagtttttac aaaatagtaa
    13621 actaaaagga gttgcagcga gactgtgact cctttttggc tttatttatt taaaataaaa
    13681 agaagttttc cggaaatttt tatttaactg tcgtgtacga agaaaagttt gctataatgg
    13741 aaaaaagcgt gcaaagaaag agggagtttc attatgatgc tacgtgtaga aaaattgcgc
    13801 aaaaaaatgc aagaagaaaa tttggattca tttttggtta ccagtccgta caatcttcgt
    13861 tacttaacga actttacagg tacgacagga ttagctgtta ttactttgga aaaagcattt
    13921 tttattactg attttcgtta taccgagcag gcggcagcgc aggctcaagg gtttgaaatt
    13981 attaaaaatg tcggacctat ttttgatgag gtagcaaact tagtacgcaa agaagaactg
    14041 cactcattag cttttgagga aacaacagtc tcatttttag aatattcggt tttagaagaa
    14101 atcatcgatg cggaattagt acctgtttca cagatgatcg aagaactcag agaagtgaaa
    14161 gatgaagaag aaatcgctat tatcgaaaaa gcatgtcata ttgcggatat ggcatatgat
    14221 cacatcctga aaatgatcca accgggcatg actgagattg aagtcgcaaa tcaactagac
    14281 ttttatatgc gttctcttgg cgcttcaggt gtttcttttg atacgatcgt tgctagcgga
    14341 ttgcgttcgg ctatgccgca tggtgtagca agcaaaaaaa tcatcgaaca aggtgacttg
    14401 atcacattgg actttggttg ctattacgaa gggtacgttt ccgatatgac acgtacattc
    14461 gctattggtg atcctgggga aaaattaaaa gaaatttatc aaatagtctt agatgctcaa
    14521 ttagcagtta ttgatgctgc aaaaccagga atgactggtg tccaattaga tgcagttgct
    14581 agagattata tttcaaaaca tggttacggg gaagcatttg gacattctac aggacatggg
    14641 atcggtttag agatccacga aggaccaaac gtttcttctc gtgcagaaaa gcaatttgta
    14701 ccaggaaatg tgatcactga tgaaccaggg atctacttac caggaatcgg tggagtacgt
    14761 attgaggatg acttgttgat cacagaagat ggaaatcgtg tgttgactcg ttcaccaaaa
    14821 gaattgatca tcttatagtt tcatcaatag aagaagtaag cgtaaaaatc agtgttcaca
    14881 gactttgtta tagaaaatca ctcgatttaa tgatacacta tagaagaact tttaagctgt
    14941 aagagataat aaagttgggt gttttctcca attcatagca gtttgcagct gatcggcggc
    15001 agttttctta ccacgtgatc actatgccgc atcatagaga attcggatgt atctcgtgct
    15061 taggcaaagg gaggaataaa taaatggcag atgaaaaaaa tttgatttta aacgcaaacc
    15121 aagatttagg agagatcgtg atcgctcctg aagtcatcga agtaattatc gggattgcag
    15181 cttctaaagt agaaggcgta tacggaatgc gcggaacttt tgccagcaat gtcactgaat
    15241 tattaggacg tgcagctcac ggaaaaggtg tttacttagc aaacgaggaa gatggcttga
    15301 aagtagatct ttactgttat ctagaatatg gtgtttcagt tcctaaagta gcaatggaaa
    15361 tgcaggaacg ggtcagccag caagtccttt tcatgacgga tatcgattta tctgaagtca
    15421 atatccatgt agtagctgtt gttcctgaaa aattagaaca accaacaatc gaagaacttt
    15481 ttgaagacga agaggaagag aatgaataaa gaattatcaa gacacgagat tcgggaaatg
    15541 gcattgcagg ctttatttcc attagacttc aatgcagacc taacaaaaga agatgcgatt
    15601 ttcaacgcaa tcgaattaga ccaccgcgac atgatcaacg aagatgaatc agagtttgtt
    15661 cctgtttatc ttgatacgct agttggcggt gtctgtgcaa aaaaagatga attggataaa
    15721 gtgattgaaa aacatttaaa aaacaattgg cacatcaatc ggatctcaaa aatggatttg
    15781 gtgatcttgc gtatcgcgat ttttgaaatg acttatgtga cagatgtacc agcggctgtt
    15841 gctttaaatg aagcaatcga gttggcaaaa acatttagtg atgaccgttc acgtaaattc
    15901 gtcaacggtg ttttatccaa tgtattgaaa gaattagaag ctggggcgta agtcctagct
    15961 tttttctatg aatcatcata aacctgttat aaatcgggaa aattctatgg tagaatgaaa
    16021 acacaaaaaa tatctgggag tggttagatg gcagaattga ttaacggaaa agaattagct
    16081 gaaaaaatgc aggcagagat tgctgaaaaa atccaaaaat tgaaagataa tggtatccat
    16141 cccggattag tcgttttatt ggtaggggaa aatccagcca gccaaatcta tgtaagaaat
    16201 aaagaacgtg tagcaaaaga aatcggaatc cattcattgg tagagcgtta tcctgagacg
    16261 atttctgaag acgaactact tgcagaaatt gaaaaataca atcaagatcc tagttttcat
    16321 ggtatattgg tccaattacc attgccaaag catatagatg aagaaaagat cctgttagct
    16381 atccgccctg aaaaagacgt agacggtttt catccgatga atttaggcag actccttgcg
    16441 ggtaaaccgg acaagattcc ttgtactcca tacggcatca tgaaaatgtt tgaagcttat
    16501 tctatcgatc cggcgggtaa acgagcagta gttatcggac gaagcaatat cgtcggtaaa
    16561 ccgatggctc agctactctt gatggcagat gcaactgtta ctgttgctca ttctaaaacc
    16621 gaaaatctgg ctgaactggc acaagaagca gatattttag tcgtagcaat aggacgtgga
    16681 cattttgtca caaaagaatt cgtaaaacca ggtgctgtag tgatagatgt agggatgaat
    16741 cgtgatgcta acggtaagct gatcggtgat gtgaaatttg atgaagtgga gcctattgca
    16801 ggctggatca ctcctgtgcc aaagggtgta gggccaatga ctatcacgat gctgatgtat
    16861 caaaccgtaa aatctgccga agcttttgga gcaggtgaat agatggcaca agaatattta
    16921 actgtcacta cattaacgaa gtatttgaaa agaaaatttg atgccgatcc ttatttagaa
    16981 cgcgtatatt taacaggcga aatatcgaat ttccgtttac gtgccaatgc ccatcaatat
    17041 ttcagcttaa aagatgacca tgcaaaaata tctgctatta tgttcaaagg agcattccaa
    17101 aaattacgat tccagccaaa agaaggaatg aaagtccttg tcgttgggcg tatttcatta
    17161 tatgaagcgg gaggttctta tcaaatctat gtagagcaca tggagcctga tggaataggt
    17221 gctttatatc aagaattgga agaacgaaaa gaaaagctga tgaaggaagg cctttttcaa
    17281 gggcctaaaa aaatgctgcc acgtttccca aagcgaatcg cggtccttac cagtccaagc
    17341 ggagcggtta tccgcgatat catcacgacg gtgaaaagaa gatatccaat tgtccaattg
    17401 gttttgttcc caacagttgt acaaggaaac caagcagcgg acaatgttgt caggaatatc
    17461 cagagagtag aagagatggg caatttcgat acaatgatca tcggtcgtgg gggtggttcg
    17521 attgaggatt tatggccgtt taatgaagag cgggttgctc gggcgattta tcaagctaat
    17581 acgccggtga tttcatctgt aggacatgaa acagatacca cgatcgctga tttagtagca
    17641 gatgttcggg ctgccactcc aacagcagct gcagaattag ctgttcctgt tttgtcagaa
    17701 gagttgatgc gcattcaaga acggcaagct agattgcagc aggcgtttgt acgccagatc
    17761 cagcgcaaac aagaacggtt cgagcgtgct cgacgttcct atgtatttcg gcagcctgaa
    17821 agattgtatg aaggtcagac catcaaatta gaccagttga aacagcgatt atatcaagct
    17881 acacagcaaa tttatcatgc gaaagaaaaa caaacgatcg cattaagcca ccaattggaa
    17941 caagtggctc cgctttatcg agttaaagca gctcgccaag aaactgctta tctagaaaaa
    18001 cgcttagaag agaaaatcca acaatatatg aaacaacagc agcagcgttt tgctcaagct
    18061 gttcaatctc ttgatttgtt gagtccattg aaaatcatgg gaagaggcta cagctatacg
    18121 acgttaaatg atcaagtagt aaaaagtgtc accgagattc atcaagggga tttgctgacg
    18181 atccattatc aagatggaat cgttaaagga caagtacaag agatagacaa aggagaataa
    18241 aaatatggaa aatccaactt ttgaagagtc actgcaagaa ttagaaaaaa ttgtcatgca
    18301 gttagaacaa ggagacgttc cattagaatc agctctagat tcgtttaaac gaggaatgga
    18361 attaagcaaa cattgccaag atacattagc taaagcagaa gagaccttga cgaaaatgat
    18421 gacagaatca aatgaagaag ttgattttga cggaaacgag gaaagctgag atgagattaa
    18481 ctgacttttc agctattcat ctacctgctg tagaaaaaga aatcttgtct tttttagatg
    18541 agcatacaac ggatagagga ctctatgatg cgatgagtta ttcagtgaaa gcaggcggga
    18601 aaagaatacg tcccttatta cttttaacgg ccgttgcttc ctttgatgag cctattgatg
    18661 ttcccgtcta tcaagtagca gctgctttag aaatggtgca tacgtattcc ttgatccacg
    18721 atgatttgcc tgcgatggac aatgatgatc ttcgtagagg aaaacctacc aatcataaag
    18781 tattcggcga agcattagct atattggccg gagatggttt gcttacagga gcattccagc
    18841 taatcagtat ggcacactta ggtaattctc ctaagcttct gctgcttcag caattagctg
    18901 tttgtgcggg atcacaaggt atggttgctg ggcaagctgc agatatcgag ggcgaatcga
    18961 aaaagctttc tttagaagaa ttagcattta ttcatgaaag aaagacaggg catttgattc
    19021 gttatgcctt gctggctggt ggcatattag cgaagcaacc tgaagaaatc ttattgctac
    19081 ttcaacgatt agccgaacat ttaggtttag cttttcagat aagagatgat ctactcgatg
    19141 tgattggtac aacgaaaaca ttagggaaaa cagctggtaa agatgaacgt atggaaaaaa
    19201 atacttaccc gcgtttatta ggcctagaaa agacacgtga ggcgttggag attgagctgt
    19261 tatccgccaa caagatcata gacaaattag aagaggacgt tttttctttt gatggcgggc
    19321 tgatgcgcca gatgatcaag caatttgatg tggagaaatg agggaaagaa tggaaaaaga
    19381 acgcgtagat gtattagcgg tcaaacaagg attatttgaa acaagagaac aagccaaacg
    19441 ttcagtgatg gctggtttga tttacaacga aaaaaacgag cggtttgata aaccgggaga
    19501 aaaaattcca gtgagtagtg aattaagagt caaaggtaaa aaacttcctt atgtttctcg
    19561 agggggatta aaattagaaa aagcactcaa agcatttgat ctatctgttg aaggaaaaac
    19621 attgttagat attggtgcat ccactggcgg atttactgat gcagctttgc aaaatggcgc
    19681 aaaaatgagt tatgctttag acgttggcta caatcagcta gcttggaaaa taagacaaga
    19741 tcctagagtc gtcgtaatgg aaagagtgaa ttttcgttat gcaaaacccg aagactttac
    19801 cttaggaatt ccagaagttg ctgttatcga tgtttctttt atttcactaa aactgatgct
    19861 gcctccatta catgctattt taaaagaaga gggagaagtg attgctttaa tcaaacccca
    19921 attcgaagca ggcagagaag ctgtagggaa aaacggaatt gttcgtgacc ctcagaccca
    19981 tcaaaaagtt ttagaagaca ttttgtcatt tgcggcacaa ggtggatatg atgtgctgga
    20041 attaagttat tctccgatca cggggggaga aggaaatatc gagttcttgg ctcacttgag
    20101 gaaagtaccc gaaagcggaa caatcaattc agctattaat atggcagaag tggtttcaaa
    20161 tgcacacgaa cagtttgacc ataaataaat tgtaactgtg aaaatcaagt gagaagtatg
    20221 cggattcttc tttattatac caaggaaata agctatcatt gattaagaag aatatgctaa
    20281 ggagcgaaga cgtaatgaga aaaaaagatc ggcatcgttt gattactcga ttgttgagtg
    20341 agcaagatat ccgaaagcag gaagaatttg ttgaaatcct gaaaagcaaa ggaatatctg
    20401 ttactcaagc tacgatttcc agagatataa aggaactgaa actgatcaag gtaccggctt
    20461 cagatggcgg ctaccgttat agcataccag ctgaaacaaa cgaagatgta aatactaaat
    20521 tggaaaaact attaaaagac gctttcgtgg cagttgacca aatggaaaaa tttgttattt
    20581 tgaaaacatt gccgggaaat gcttcggcag cagcaaattt gattgataaa cgttacaaga
    20641 aagaattatt ttcgattatc aatgatgacg ataatgtttt gatgattaca cgaactgaag
    20701 accaggcagt tttattgaag aaagacttcc tgcattacct ttaagttgag gagcgaatgt
    20761 gatgctacaa gaaatcagta ttactaactt tgcgattatt ccggaattgc ggctttcttt
    20821 tcacgaagga atgaccgcgc tgaccggaga gactggtgcg ggtaaatcca ttattatcga
    20881 tgctttaggt ctgttagcag gaggaagagg ttcaagcgat tatatacgtc aaggagcaga
    20941 aaaatgtgtg cttgaagggt tgtttgaact accaaaacaa gaaggattct ctgaactgat
    21001 ggttgaatta gggatcgaaa cagatgagga caatctgatt gttcgacggg atatgtcctt
    21061 aactggaaaa aatgtttgtc gagtgaatgg tcatattatc acattagcta atctgagaag
    21121 aatcggcagt tatttagtag atatccaagg acaaaacgaa catcaggaac tgctccagcc
    21181 tgaatcacat cttgctttat tggatcgttt tggagatgta gctttccaac agaagaaaaa
    21241 gagttatcag caagaatata tctcttatcg tgagctggaa aaaagggtac gaaaagtgca
    21301 gcaaaatgaa aaatcctatg ttcagagaat agatatgctc catttccagc aagaagaaat
    21361 agctgctgct gatttgcaag tcggagaaga agagaaatta aaagaagaaa gagaaaaact
    21421 tagcaattat caaaaaattg ctgatggatt agctgcaggt tacggagcac tgacgaataa
    21481 tgaacaaaac agtgtagatg gtgttggctt ggccgtttca gaactgcaag ggatcgccca
    21541 tctagatgtg gagtatgaag caatttatga aaatatccaa agtgcttact atttgctgca
    21601 agatgcaatc ggcgatatga gtcgtcagat tgatttattg gagttagatg agagtcgttt
    21661 agaagaagtt acgcaacgtt tagaattaat ccgacagttg aaacgtaaat acggcgaatc
    21721 cattgagtca atattggctt actatgacga aatcaccgaa gagctagctt cttctgattt
    21781 ttcagaaggt caattggaca aaatgaaaga ggaactagag caaaaagagc ttctacttca
    21841 gcaacaagcc gcagatcttc atgaagcgcg taaaaaaatc gcaaaagaat tggaaaaatc
    21901 cattctgcat gaattgaaaa gtctgtatat ggaaaacact gagtttgagg tacgtttctt
    21961 aaaagaagaa aatagacaat tgaatcatga tggatttgac caaatcgaat tttacattac
    22021 gaccaatcca ggagagccac tgaagccttt ggtgaaagtc gcttctggtg gcgaactgtc
    22081 tcggatgttg cttgctttga aaactatttt ttcttctgag caaggtgtga caagtattat
    22141 ttttgatgaa gtggatacag gtgtgagcgg aagagttgca caggccatcg cagataaaat
    22201 ttctcaaata tcgaaatact ctcaagtttt gtgcatcact catctgcctc aagttgctgc
    22261 agtcgccgac tatcaatatt atattgtgaa agcagtgatt gatggacgta cgcaaacttc
    22321 tgtttcagag ttgaaaacaa aagaaagaga agaagaaatt gcacgtatgc tggctggaag
    22381 tgagattaca aaactcacac tcgaacatgc aaaagaacta ttgaaactcg caaaaaggac
    22441 ctgagccacc tcgcagtggt tcaggtcctt ttcagcagct gatagctata ttttattttt
    22501 acatgacact cattacagca gtcaatacaa cagatccaat cgttaaaatc agcattgtcc
    22561 aaatgacaaa ttttgttact ttagcaaaag tacttgtttg ttttttttca cgcattgttt
    22621 tcaacctcgt cattaattag ttgattcaag tgtacaatct tttttccatt tccacaagag
    22681 ggctttttta taaaaacttc ccttttttta aataataggt agttacgata caaataaata
    22741 atgttaatag aactagccat ataaaggcat catccctttc agcaataggt aacttaatgt
    22801 tcattccata gatactgccg acgattgtcg gtatagttaa aataatcgtt aaagaagtta
    22861 aaattttcat aatattattc agattattcg atacgattgc tgaaaaagtc tgattcattt
    22921 gttctactag ttttaattga atgcgagaag tagtcattgc ctgctctgtt tccaccaata
    22981 catcacgtaa atgatttttc aaagcatgat tttcttggaa aagtttattt tgcgctagtt
    23041 ctttcaatgc atctaaatta gcagaagtcg cttctctgaa ataaacgagg cttttttgga
    23101 tatccatgat ttgataaagt tgtttgtttt cagtggatac ttgaagtttg gcttccaaag
    23161 tatcgcattg agattgaagc gtagctaaat aactgttgta agagagtgtg acctgccaaa
    23221 tgaattgaaa gaagatattg agattaggcg aaatatcact ttttggaaag gtctggctaa
    23281 agactttctt taaaaacagt ggttcatgat tcgtaattgt gataagcttc tttttgggcg
    23341 tcaaaataat agagagcggg tatgtatcca cttgaaaata accgctagga cttgttttag
    23401 caaaaggata ttgtaacaat aataacgctg gctttgtcaa ctttgattga ttgaattctt
    23461 ctgtacggga attttctgca tcgtccaata tggaagtgat gtaatctgac ggaatttcaa
    23521 attgatgtgt cagctgatga atctcttctt gattaggacg ttctacacaa taccagatcg
    23581 tttggtcagt ttcctttccg gtattacgca gtttctcatt atctaatgta aaatatttga
    23641 tcatgatttt cctcctgttg cctgtttctt ttattataac ggatagggaa ttggaataca
    23701 tggaatgtgt ttgtaagata tagaatataa ctgcataaat ttaaataaaa tgaaacagtt
    23761 gtgtaaagta tatacagtat acttgaagca acgataaaaa aggagtttac gttcatgggc
    23821 acattcacac cacttatctt gttttggtat atttacccgg taatcgtttt gtttggttgc
    23881 caatttcttg taaaattatt ttcgttaaat cgtcgtttta aaataaaagc ccctgatttg
    23941 gcgattcctt ttttgtgggg cggtatgcat gcattgtcta gaaatacagg gacaatttct
    24001 atcttaccgt ttttctttat ttctgttttg ctaatgggaa tttgtattgc tgttttccaa
    24061 gcatattatt atgaagaaat tatttatccg cgttatttca agatgacttg gcgtttggtt
    24121 tttctgttga caatcgtctt gtacgctgtc ttgatcattg tgaacatact ttcttaccta
    24181 tgaaaaaaat agaaaagctg taaaaagtct tttaaaaggc tttttgcagc ttgattttta
    24241 aaaaaatgtt tatgaataaa aaaagttgtt cccggatttc ccagaaaagg tggtagaaag
    24301 tgggggattg tggtagacta attttagtta gtggaggaat ggggggcttt caatgttgat
    24361 gggtgaattc caacataata ttgatgcaaa aggacgttta atcgtgccct caaaacttcg
    24421 tgaagaactt ggcgaaaagt ttgttctgac acgcggattg gatggctgtc ttttcggcta
    24481 tccgatgagc gaatgggaaa atctggaagc taaactgaac gaaatgccgt tggctaaaaa
    24541 agacgcacgt acttttgtgc gattcttcta ttcggctgcc actgaatgcg aacttgataa
    24601 acaaggacga atcaatatac ccagcacgtt aagaaattat gcagctttga caaaaggttg
    24661 cgtagtcatc ggtgtatcta accgaattga aatttgggat gagacacgat ggcaagattt
    24721 ttctgcagca gcagaagaga actttgacga aattgctgaa agtatgattg atttcggact
    24781 ataagaatag gaagaggctg actaatgact gaaacatttc aacactatac agtcatgttg
    24841 aaagaaacgg tagatggatt gaacatcaaa ccggatggcg tttatgtaga ctgtacactc
    24901 ggcggtgccg gacatagtga atatttatta agccaactaa gttctaaagg gcatctgtat
    24961 gcgtttgacc aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc ttcttatgtc
    25021 gaacaaggta tggtcacatt tatcaaagcg aacttcagag aattagaaga acgcttgagt
    25081 gagcatgtag ataaggtaga cggcattttg tatgatctag gggtttcctc tcctcagcta
    25141 gatgaagcag aacgtggatt tagttatcat caagatgcac cgttggatat gcgcatggat
    25201 cagtcagctc ctttatccgc ttatcacgta attaatgaat attcctacca tgaattagta
    25261 aagattttct tccgctatgg cgaggagaaa ttttccaaac aaattgctcg tgaaatcgaa
    25321 cgagtacgaa aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat cattaaacaa
    25381 gtcatcccag ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat ttttcaagct
    25441 gtgcggattg cagtaaatga cgaactcggt gccgaagagg cctctttaga acaggcaatc
    25501 cggttattga aagtcaatgg aagaatcagc gtaatcacat tccattcatt ggaagacaga
    25561 attgtcaaaa gtatgtttaa agaatatagt acggtacaag atctaccacc gggacttcct
    25621 gtcatacctg acgaattcca accagaactg aaagtcatta ctcgtaaacc aatccttcct
    25681 tcagaggaag agctaacaga aaacaatcga tcacgcagtg caaagttacg tattgcagaa
    25741 aaaataagaa tgaaagagga gtagaatgat ggcagaatta aagaaaatgc aagattacga
    25801 atatgattta cctgaattgg tggatcagcc ggaacaacaa atgcctgatt cttcttcgaa
    25861 tcaagaaatc attcttccac aatcaccggc aaaacgatta aagcagattt ctgctttgga
    25921 aaaaattgct gtggcaagcg tggcgattgc tattgttgct ttatgtgttt taacagttat
    25981 gctgcgcaca aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa cagaaatcaa
    26041 tgataaaaag caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt ctcgtactga
    26101 acggattaaa caaatcgcag aagaaaaagg attgtctatt aatgacgaca atttaaggaa
    26161 agtgaagtaa atgagtctta aaaataaaat tcggcggttt atggaaaaga aaaatctaaa
    26221 tccgatgaac aatcgcaaaa aagtaggtat cattttattt gctacgagta ttggattgtt
    26281 ctttttattt gccgtccgtt tctcgtatat cgttattgga ggtcatgttg ctggtacctc
    26341 attagctgaa aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag caaaaagagg
    26401 aacgatttat gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt attcaattaa
    26461 agcgatactc tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg aagaaaagaa
    26521 ttttgataaa atagcagaaa tcctgcataa gaatctttct atagacaaaa aagatgcact
    26581 gaatatttta gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg gtagctatgg
    26641 gaaaaatatc tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa aagaaggtgt
    26701 cgctggtctt tattttgtcg accatcaggc tcggatgtat ccaaatggcg tcttttcttc
    26761 tcattttatt ggatacgcag ttccagacaa agatgagaat ggcttagttg gtaaactagg
    26821 tttggagtct gcttacaatg atatcttgag cggtaaagat ggtaagatta tttatcaaaa
    26881 agataatttt caaaatcctc tgccaggtac agtggcagaa gaagaaaaag ctgtcgacgg
    26941 acaagatatc tacacgactt tagacagcag actacaaagt tatttagaaa cactgatgga
    27001 tcaagtaaat gaagaatatc agccagaaga attgacagca gtcctgatga aagcaaaaac
    27061 cggagaaatc ttggcaatgg gacaacgacc tactttcaat cctgaaacaa tggaaggatt
    27121 gactggaaaa gatgctatct ggcgtaattt cttggtacaa gatagttatg aaccaggatc
    27181 aaccatgaaa gttttcacta ctgcagctgc tatcgaagaa ggagaattca acgagaatga
    27241 aaccttccaa tctggaaaaa tccaagtaga agatgctacg atcaatgacc atgactttgg
    27301 tgaaaaaggt gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg tcgggatggt
    27361 catactggaa cagcgattag gcggacgatg gtataactat ttacaaaaac tgggatttgg
    27421 acaaagtact cattctggat tagatgatga agtaaacggt gctttaccta cattaaatat
    27481 tgtcgaccga gcaatgagtg cttatggaca agcggtaggt gtgacgaatt tccaaatgat
    27541 gaaagctttc acttctattg cgaataacgg tacaatgatt caacctcgat atatcagcaa
    27601 agtggtggat ccccaaactg gagaagagcg aacaactcaa acagaagttc tagggcaacc
    27661 tttttcaaaa gaaacaacag aaaaagtccg tgaatatatg agagatgttg tcgaaagcga
    27721 aaactacggt agtgcttatg gtgtatatag tgtacctgga tacaatgtat cggcaaaaaa
    27781 cggaacagcc caaatcgctt ctgatacagg gggatatcaa acaggagata cagcctacct
    27841 gtattcgatt gtggagatgg ttccttctga agatccagac tatgtcttgt atctaacaat
    27901 gaaacaccct aagacatatg atcgaatggc attggctaaa atcgcaaatc cattgatgaa
    27961 acgggcaatg gattttaaag aaactgaaga agattcagat acagaaacaa aaacagaaaa
    28021 agtatccgta gccgattatc gtaatcttga agcggacgtt gcagcagctg atgctcaaaa
    28081 aagtggactg caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac aatctactgc
    28141 caacggtgat cagttgatct ctggagaaaa actgatccta tatactggtg gtgataagct
    28201 gatgcctgac gtaacaggat ggtcaaaagc agatatcatg aagctgggga aaatcctcgg
    28261 tattgaagtt agttttgatg gagatggtta ttgtgtcaaa caagaacttg ctccatatga
    28321 aaaaatcact gaagacaaac ttagttttac tttagaagaa taagaagaga aataggagag
    28381 aaaagagcat ggaatggaca caagcattga ttcccattgt cagcagttgt gcaatgacaa
    28441 ttgccgcaat gccgctattt attggttatt ttcaaatgaa aaagcaaggt caggcgattc
    28501 gtgaggaggg accaaagtgg cacaatagca aagccggaac tcctacaatg ggcggcttag
    28561 tgtttctgat cggctctatt ttgacaggaa tttgggttgg agcttggcaa aaacagctaa
    28621 ctcctactct tttcatcttg ctttttgtat tagcgttata cggagtaatc ggctttcttg
    28681 atgattttat aaaaatattc aagaaaagaa acatgggact gaactcgaag cagaaattga
    28741 ttggacagat tctaggggga atcatctttt atcttgttta ccgttctgaa ggttatccag
    28801 gtgtattgaa tttcttcgga ctagatctac ctttaggaat catttatggt attttcgctc
    28861 tcttctggct tgttggattt tcaaatgcag taaatctgac agatggcatc gatggattag
    28921 ttgctggctt aggaagtatc tcttttgcag cttacggtct aattgcttgg catcagcatc
    28981 agtatgatgt attaatcatt tgcttaagtg ttttaggagg tttactgggt ttcttcgtct
    29041 acaacagaaa acctgccaag atttttatgg gagatgtcgg ttcattagcg ttgggcggac
    29101 ttttagcggc aatttctata atgctaaacc aagaatggac attgcttttg attggtttga
    29161 tctatgttat ggaaacagca agtgtgatgc ttcaagttac ttcctttaag cttacaggaa
    29221 aacgaatttt taaaatgtct ccaattcatc atcactttga aatgtgcgga tggtcggagt
    29281 ggaagattga tacagttttt tggttgatca gcattgtgac ttcgttgatc acattatgga
    29341 tcgtttggta ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa taagaaagtg
    29401 cttgtcttag gactcgcaaa aagcggagta agtgcggcca aattattaca tgaattgggc
    29461 gcacttgtca ccgttaatga tggaaaacct tttgacgaaa atccggaagc acaagagctg
    29521 ctttctttag ggataaaagt aataacaggt agtcatccaa tcgaattgtt ggatgaagaa
    29581 ttttcattga tggtcaaaaa tccagggatt ccctacagtc atcctttagt agcaaaagcc
    29641 caagaaatgg gtattccggt aattacggaa gttgagctag cttacgaagt agcggaatgc
    29701 ccaattattg ggattacagg aacgaacgga aaaacaacga caacaacgat gacaggacta
    29761 ctactgaatg caggagcaga tcaaggaatt gcacgtttag cgggtaatat tgggtatcca
    29821 gcaagtggtg tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga attgtctagc
    29881 ttccaactca tgggaatcac agatttccgc cctcatatag cggtgattac caatatctat
    29941 gaagcacata ttgattatca tggtacaaga aaagaatatg tcaaagctaa atggaacctt
    30001 caaaaaaaca tgacagaaaa agattatctg atccttaact ggaatcagtc agaactacaa
    30061 gaattagcgc aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga agtgttggaa
    30121 gacggtgtgt atgctgacga ttactcaata tactataaaa aagaaaaaat aatggaaata
    30181 tctgaacttg gcgtaccagg taagcataat gtagaaaatg cactagctgc tatttctgta
    30241 gcaaaattgt atggtatatc aaatgaagct atacgagaaa cgttgcattt tttccatggc
    30301 gtaccacatc gtacgcaata tgtaggagaa atccaaggca gaaaatttta taatgattca
    30361 aaagcaacca atattttagc gactaaaatg gctctaagtg gatttgaaac gtctaaggtc
    30421 gtgttattgg ctggcggatt agacagagga aatacatttg atgaattaat tccttcatta
    30481 aaaggaatca aagcaatggt cgtatttggc caaacaaaag aaaagttgat ggatgctgga
    30541 aaaaaagcag gcatcgagac aatagtaaca gccgattctg tagaacaagc agttccttta
    30601 gcattagaaa acagtacaga tggtgatgta gttttattat caccagcgaa tgccagctgg
    30661 gatcaatacc caaattttga aactagagga aacagattca tggaagctgt gaaccgatta
    30721 aaatagaaaa agtagaagcg agcgataaaa aatgaaaatt ttggttacag gcggaggaac
    30781 agggggacat atttatccag ctcttgcctt tgtcaattat gtaaagactc aagaaccgaa
    30841 tgctgaattc atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg tccctgatac
    30901 aggaatgcct ttccatacct tggagattca aggatttaaa cgaaaaattt ccatgcataa
    30961 catcaagacg gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa tcctcagtga
    31021 ttttcaacca gatatcgtta tcggtactgg aggatatgtt tccggagcgg tagtgtacgc
    31081 tgcagccaaa atgggtattc caacagttat ccatgaacaa aacagtgtac caggtgttac
    31141 caataaattt ttaagccgat atgtggatcg gatcgctctt tcatttaaag atgcggcttc
    31201 cttttttcct gaaaacaaag cagttcttgt aggtaatcct cgagcacaag aagtagcaga
    31261 tactaaaaaa tcagatattt tgaaaacatt tgatttggat ccagaaaaga aaacagtctt
    31321 gatttttggc ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag tttttttaag
    31381 tgaatatatt ccttcttctt accaagtttt gtatgcttca ggagatagat attatgaaga
    31441 gatcctaaat aaaataaatc ctgaaataca aaatattagt gtccagcctt atatcaaaaa
    31501 tatggcagag gtcatggctt ccagtgatct gctggtgggg cgtgctggtg caacttcgat
    31561 cgcagaattc acagctctgg ggttaccggc agtgttggtt cccagcccat atgttacgaa
    31621 tgatcatcag actaaaaatg caatgagtct tgttcacgca ggagcagcaa aaatgattgc
    31681 agataatgaa ctaacaggtg aatcactaag tcagacagtc aacgaaatca tgggtgatga
    31741 agaattgcaa aagcaaatgt gccgagcttc taaagaacaa ggaatccctg atgcttcaaa
    31801 acgtctttat gacttagtaa aacaaataat caaaaaataa aggcggtggt cttagtgagt
    31861 aagaaaaaag aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa tttgactcct
    31921 tggcaaaagg ctaaccgaaa atatctggca gaacaagaga tgaaaaacca agagccaaaa
    31981 aaagaagaca taacattaga tacacccctt tcagattctg aagaagaaaa aacggttcaa
    32041 gaggaacagc aagaagaaag ccagtccgag aaaaaaatcg aattttttga agagatcaag
    32101 caaaagaaaa acggtggacc atataacggt tcttttttga accggctgcc caatttaaaa
    32161 agtcaacggc acaaagtact atttcgtcgg ttagctttga tcatcactgt attgggtata
    32221 ccgctgattt ttttgatcta ttatgtttct ccttatagca aattacaagc agttgcagta
    32281 tcaggcaata agacagtcaa cagccaagaa atcatctctg atacaaaatt gtcattagga
    32341 gaaaatgttt ggggacagta ttttcatcga tctacttata tcgatcgcct taaaaaagca
    32401 cagccaagaa tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt cgatttggat
    32461 gtaacagaat ataaagagat tgctttgatc gcaaaaaaca atcaatacta cccagtgatt
    32521 gaaaatggaa cagtattaga tgaaaaagta gcaaatccta caaaaaatct ccctatcttg
    32581 gaagagttca aagactctgc aaaaatcaaa gaattaacaa agcaatacaa tcagttatca
    32641 tcagaattac aaaaagcaat ttctgaaatc aagtacacgc caagagcaag caataaaaat
    32701 ctgatccaat tgaatatgaa tgacggcaat caagttattg taaatatcaa taatcttgcg
    32761 aaccaaatga aatattattc gcaagtagcc aaagatatgg atgaaaaagg tgtgatcgac
    32821 atggaagttg gtatcttctc gcatccttac ggtagcactc aagaaacgga agaatcgggc
    32881 caatcggaag aaacaactga aagtgaagaa aatgcggatc aaacaaacga atcaagtgaa
    32941 gaggcagaaa cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg atcatctgat
    33001 tcaaatgatt caagtgaaac aagtcaaata gaagataatc caagtacaac tccgtcaagt
    33061 ttaaatgaaa attgataaaa caactttaaa aagtaagaat gtactttcta aaaaccagta
    33121 atttatggta gaattggaag cagtgaatat atctattaat aagtatattt taaaacggac
    33181 gctagttgtt cgcttgtata tggaatagta agtaggaggg aatcccattc atggcaaaaa
    33241 caggaatgta tgtaggcctt gatattggaa cgacatctgt taaagttgtc gtggctgaat
    33301 atatcgacag ccaaatgaat attattggag taggaaacgc aaaatcagaa gggattaacc
    33361 gaggcattat cgttgatatc gataaaacgg tccaagcgat acaacgagca gtacgacaag
    33421 cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca gcaaacatgc
    33481 ttgaagtaga aaattgccag ggaatgattg cagttaacgg agattcaaag gaaatcaccg
    33541 atgaagatgt cagaaatgtg gcttcagctg cccttgttcg ttcaatacct cctgaacgtc
    33601 aaatcgtgtc aatattgcca caagatttta ctgtcgatgg gttcgaggga atcaaagatc
    33661 ctcgtggaat gatcggcgta agattagaaa tgtacggtct gttgtttaca ggacctaaaa
    33721 cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc aacgaaatgg
    33781 tgattactcc tttagcgctg actgaatcaa ttctttctga cggagaaaaa gattttggta
    33841 cgatcgtcat tgatatgggt ggcggccaaa caacaacggc tgttatgcat gataagcaat
    33901 tgaagtttac caatcttgac caagaaggtg gcgaatttgt cactaaagat atctctattg
    33961 tattaaatac gtcatttaac aatgcagaag cgttgaaaat aaactacggg gatgcatacc
    34021 cagaacggac atcaccagat gaagaatttc cagtagatgt gattggtcag tcagaaccag
    34081 tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa cagattttca
    34141 acaaagcaaa agatgcattg gaccaaatcg aagcattaga gttgccaggt ggcgtcgtat
    34201 tgacaggcgg agctgctagt cttcctggtg ttgtggactt ggcacaagaa atcttcggcg
    34261 taaatgtaaa attgtatgtt ccaaatcaca tggggctgag aaacccggta tttacaaatg
    34321 taataagtat cgtggattat tcagcaaact tgagtgaagt gtatcaattg gcaaaaattg
    34381 ctgttacggg tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa acaacaaaca
    34441 catatgagag ctatgaagca cctgaagaaa caatctacga tgagccggaa ccaaaagagt
    34501 ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac aaatattttt gattaattca
    34561 tcctgggagg aataagatat ggaattctca attgataata atatcaatga cggcgcagta
    34621 atcaaagtca tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat gattgaagaa
    34681 aacgttaaag gtgttgaatt cattacagcc aacacagacg tgcaagcatt aaaaaattca
    34741 aaagctgaaa cagttatcca attaggcccc aaatatactc gtggtttagg tgctggttct
    34801 cagccagaag ttggacaaaa agcggcagaa gaaagtgaac aatccttacg cgaagcttta
    34861 gacggtgcag atatgatttt catcactgca ggaatgggtg gaggaaccgg tacaggtgct
    34921 gcaccaatcg ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg tgtcgtgaca
    34981 cgtccattca cttttgaagg accgaaacgt ggccgttttg ctgcagaagg tatcgctcgt
    35041 ttgaaagaaa atgtggatac attattaatt atctcaaata accgtttatt agaagttgta
    35101 gataaaaaga caccgatgct tgaagctttc cgtgaagcag ataatgtttt acgacaaggt
    35161 gtacaaggta tctctgattt gatcacagcg ccaggttacg ttaaccttga ctttgctgac
    35221 gtgaaaacag tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt ggctagtgga
    35281 gaagatcgcg tagtagaagc aacgaaaaaa gcaatctcct ctccattgct tgaaacatct
    35341 attgatggag ctgaacaagt acttcttaac atcacaggcg gtttggatat gacattgttc
    35401 gaagcacaag atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt aaatatcatt
    35461 ctaggtacat ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt catcgcaaca
    35521 ggtatcgatg aatcaaaaaa ggaacgcaag tctagccgac cagctagaca agcacaaatg
    35581 caatcacctg ctcaaaagac tgtattagat atggatcaag ctaaaccaac ttcttcagaa
    35641 gaagaaaaca gttttggcga ttgggatatc agacgtgaac aaaatgttcg tcctagagtt
    35701 gatgattcca attttgacaa tatcgaaaaa aaagaatttg atacattcaa ccgcgaagaa
    35761 acaaaatctc atggcgatga tgaattaaat acgccaccgt ttttccgtcg taaaaaataa
    35821 aggagaggaa gagcacatga ttgctgacaa cttgcagaaa atcaagcaag aaattcagga
    35881 ttcgtgtgct cttgttcatc gtgatccgaa agaagttaca ttagtagctg taacaaaatc
    35941 tgttgaatcc gctcaagctg aagaattggt tagagctggc gttatgaatt gtgcagaaaa
    36001 ccgagtagat aaattattag aaaagaaaca agatttattt gattttcctg aaataaagtg
    36061 gcatttaatt ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg aaattgatta
    36121 ctttcatgcg ttggacagtt taagattagc agaagaaatc cagaagcggt tagagaaaga
    36181 attatcctgt tttattgaag taaatgtttc tggtgaggaa tcaaaacatg gaatttcacc
    36241 agatgagctt ttaccgtttg tggaatcttt agctgcctgc acaaaaatca aggttgttgg
    36301 gttgatgact atggcgccta aagaagcttc tgatgagcaa atccataaag tgtttggtac
    36361 gctgaaagaa ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac catgtagtga
    36421 attaagtatg gggatgagtc aagactttca aattgcgatt gaagaaggtg caacatttat
    36481 ccgtgtagga acagcgctat tcaaagacga ataaaggagg aacaaaaatg ccgcttttaa
    36541 acagaggagc aattgctaat tttttcggat tagctgatga ggaagagtac gaataccaag
    36601 attatcctga aacaacaaaa aaggctgtac agccaactaa aaaaaataca acacagaatc
    36661 aatcaactca ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg gcgacttcac
    36721 gtcctgtaac gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt gaactacatc
    36781 aatcacctac aaaaactgta gcgaaagaaa gaacagcttc aagaacctct acacaaccta
    36841 aagcaaatct tgctggaaaa atcactgtaa tggaaccaag agcttattct gaagctatgg
    36901 cgattgctaa acgaatcatt gctggagaag caaccttagt gaattttcat ttagtagaag
    36961 aaaaacaagc gagaagaatc gttgattttc taacaggaac agtttatgca ctagatggag
    37021 atatcaaacg tgttggagat gagatttttc tttgtacacc gactgggatg gagattgaca
    37081 gttcaaccgc tcaatctttt gcagatgcga atttgtttga tctgtaagag gaggaactac
    37141 gatttatact attgcactat tacttcattt acttcaacgg gcagcctatt tctatagcat
    37201 tatcttgatg gtatatgcat tgctctcttg gtttccaggc gggtatcaat cagggctagg
    37261 acgattatta gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc tgcctttgag
    37321 gattggaccc ttggatttca caattttagg ggcaatcctg atcttgaact tagccgtaca
    37381 aggtttagca cagatcgtta tatggataac tgtccattta gtttattgat tgaagcaaca
    37441 aaatctttat tctcagaata gataggaggg gaaaattttg gatgcaaatg tataccagca
    37501 ctttcgggca gatgaacgta catttatcga ttcggtaggc gattggattg aacaggttca
    37561 aggacaatat gcaccctatc tgaccgaatt tcttgaccca agacaatcct atattttaga
    37621 aacactgatt cgacaaagca gtgatttgcg tttcatgttt tatggcggat atgaacaggc
    37681 agaaagaaaa agatgtttga ttttccccga ttattacgaa cctgtagagg aagattttga
    37741 aacagcattg tatgaaatcc attatcctag caaatttgcc agcctaagtc atgggaaaat
    37801 ccttggaact ttagtgggaa cggggatcaa acgctcttac tttggcgata tcatctcgga
    37861 cggtgatcgc tggcaagtat ttattgcaaa tgaggtggat cattttgttg ctgcacaagt
    37921 aacaaagatt ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag aaatgattat
    37981 gccgaaagat agctggacac aagaaagagg aacagttagt tccatgcgac tagacagtgt
    38041 gatctctgag atttataata tttccagaca gcggtccaag cagctgatcg aagctggtaa
    38101 agtaaaagtg aactggacag aaaatacaag acctgatttt gtacttgaac tattagatat
    38161 tatttcagtc agaggttatg gcagagtcca gatccaagaa cttgagggga agacgaaaaa
    38221 agaaaagtac agggtactgt tcggtgtatt acgtaaataa aataaaaagg tggtttactc
    38281 atggctttaa ctccattaga tatccaaaat aagacattcc cgacaaaaat gcggggatat
    38341 aatcaagacg aagtagatga tttcttagat ttagttgttc gtgactatga ggaattgact
    38401 caaagaaatc gagaattaga aaaagcagtc aaacattctg aagaaaaact tgagtacttc
    38461 aatgaattaa aagatgcttt gaaccaatca atcatcgtag ctcaagatac ggctgataaa
    38521 gtaaaaacaa gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca aaacaaagca
    38581 gatgagttag ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga tgcagaagaa
    38641 aaagcaagaa aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc gacagaaact
    38701 gaagatctta agaaaaaaac acgagtattc catcaacgta tcagcttgat gcttgagtct
    38761 caattagaac aagtgaaaag tccggaatgg gatgaaattt tacaaccatt ctccagctac
    38821 gtgactgaca gccatgaagt gatcaaagaa gttttagcaa aacagcttga caatgaaaat
    38881 gatactgatg taaactcaga cacaggagtc atagaagcac ctatgacggc atttgataca
    38941 caggctattg atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa cagaaaagca
    39001 ctttgttatt cttaaagcga gccgatgaga gtggaagttc ggtaagacac tgcatggtga
    39061 aagatcctct acttgagacc tgcttttccg aaattagagt aagaagcagc ggctgacctc
    39121 gttattggtc acagagagtt tgcactagat agatgcaact aaatttgggt ggtaccacgg
    39181 catgttcgtc ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa taaaggagtg
    39241 tatggttgaa tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt ccctatgcgt
    39301 ggtaatttgc caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa tcgaatctac
    39361 caaaaaagac aagagttgaa cgaaggaaaa cctagctttg ttcttcatga tggacctcca
    39421 tatgccaatg gtaatatcca tttaggacat tcattgaata aaatcagtaa agacattatc
    39481 attcgttcaa aatcaatgtc tggctatcgt gcaccatatg ttccaggatg ggatacacat
    39541 ggattgccga ttgaacaagt attggctaat aaaggcgtga aacgtaaaga aatgtccatg
    39601 gcagaatact tggaaaaatg tcgtgaatat gcactttccc aagtagataa gcaacgagca
    39661 gatttcaaac gcctaggagt tgcaggagat tgggaacatc cttatgttac attagatcct
    39721 tcttatgaag ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaagg ctatatttac
    39781 aaaggattga aaccaattta ctggtctccg tcaagtgagt cttctttagc ggaagctgaa
    39841 attgaatata aagatgtaaa atctccatct atttttgtag ctttcaatgt caaagacggc
    39901 aaagatattc ttgatgaaga tacagctttt gtcatttgga cgacaacacc atggacattg
    39961 ccagcaaacc aaggtattgc tgtaaatcca aaatttacgt atgtattggt ggaagccgat
    40021 ggccgtaagt ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga aatcggttgg
    40081 gaaaatgtaa cagttctaaa agaatttgct ggacaagaaa tggaatatat gactgcaagt
    40141 catccattct atgaacgtga atcacttgtg attttaggcg atcatgttac tttagacgct
    40201 ggtactggat tagtccatac tgctccagga catggggaag atgactatat cgctggtagc
    40261 aaatataaac tatctgtagt gtcacctgta gactcaaaag gattgtttac agaagaagca
    40321 cctggatttg aaggtatctt ttatgacaaa gcaaatccta tgatcactga tctattaaaa
    40381 gaaaaaggag ctttgctgaa gctagatttc tttactcata gttatcctca cgattggcgg
    40441 acgaaaaagc ctgtgatcta tcgggcaact ccacaatggt ttgcatctat cgataaattc
    40501 cgtcagaata ttttggatga agtagaaaaa gtagagtggg ttatcccatg gggtaaaaca
    40561 cgtttgtata atatgatccg tgaccgcggc gattgggtaa tttcccgtca gcgtgcatgg
    40621 ggagtaccac ttccgatttt ctatgctgaa aatggagaag caatcatcac accagaaaca
    40681 atcgaacatg tggcaaaatt atttgctgaa catggatcaa ttatctggtt tgaaaaagaa
    40741 gcgaaagaac ttcttcctga aggatttacc cacccaggtt ctcctaatgg cgaatttaca
    40801 aaagaaaaag atatcatgga tgtctggttt gattctggtt cttctcatga agctgtttta
    40861 cgtcagcgac cagaattatc attcccagca gatatgtatt tagaaggatc agaccaatat
    40921 cgtggctggt tcaactcaag tatcacgact agtgtggcta tcaatggtgt agcaccatac
    40981 aaatcagtta tttctcaagg atttgcttta gatggtgaag gccgtaagat gagtaaatct
    41041 ttaggaaaca tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga tatccttcgc
    41101 ttatgggtat ctagtgtaga ttatgaagca gatgttcgtg tttctatgga tatcttaagt
    41161 caagtggcag aagtatatcg taaaatcaga aatacgatgc gtttcttatt agcgaataca
    41221 gaagatttcg atcctaaaaa agatactgtt tcgtacaatg atttacgttc tgtagacaaa
    41281 tatatgaccg ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg gtacgacaaa
    41341 tataacttca tgcatattta ccgtacagtt atgaacttct tgacggttga tctatcgtca
    41401 ttctacctgg attttgctaa agatgtagtt tatattgaag cagaagatga ttatcaacgt
    41461 cgttgcatgc aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt attgactcct
    41521 atcattccgc atacagcaga agaaatctgg tcattcctga aagaagaaga ggagtacgtt
    41581 cagttaagcg agtttcctgg ttatgaagag tttgcaaacc aagaagaatt gatggatacc
    41641 tggactgctt tcatggactt ccgtgataat gtcttaaaag cattggaaga agcaagaaac
    41701 agcaaattga ttggtaaatc tttggaagca aaactaactg tttatccaaa acaacaagtt
    41761 cgtgaaatgt taaaagcttt ggatgcagat attgcacaat tattgatcgt ttcaccagac
    41821 tactttgaag ttatggcagc agatgaacaa gtgccagata acgctatggt atttgatgat
    41881 gttgctatta cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg ccaagttcgt
    41941 aaagatgtag gagtagatga aaaacttcct catctatgcg gtcgttgtgc aaaaatcgtt
    42001 gaagacttct atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa gggaaaaggg
    42061 ggtgtgacat gttctttgtc acaccccctt ttgttatcaa tcgtttaatt ttccacgttc
    42121 acgataccag ttatctggtt gccagatcca ttcgaatccg tctttttcaa gtagttcgaa
    42181 agctgcttta ggtcccattg atccagcttt gtagttggga aaatccggtt gtgtttggtc
    42241 ccaagctttt cgaatgacat ccacgatatg ccaagattga gcgacttctt cccaatgaga
    42301 gaagttcgtt ccatcaccat tcaacgcatc taatagtaat ttttcgtaag cttctgggct
    42361 attttcaacc atttctgcac tatttctaaa ttccaatttg attggttccg tttcaaatcc
    42421 ttgccctact tctttcccat tcaaggataa agaaaatcct tcagttggct gaatatagat
    42481 cgttagaaca ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag atgttttgaa
    42541 tacattaaca ggaacttgtt tgaatacgat gttgatccga gtcccttttt cagtcaaacg
    42601 tttgcctgta cgtacataga aaggaacacc tgaccaacgg aagttatcga tagtgaattt
    42661 gcctgcgacg aatgtttcag tctctgattg agcatccact ttgtcttctt cgcgatagcc
    42721 cttgaattgt acaccatcca attcaccttg agcatattgc ccgcgaacga agttttctaa
    42781 cacttcttct tcgttataaa tgcggatagc tttcaatgct ttgatctttt ctgtacggat
    42841 ttctttttcg gaaaatgcta ctggcggttc cattgcaagt aatgctacaa cctgtaaaat
    42901 atgattttgc accatgtctt taagtgcgcc actatgatcg tagtagcccc cgcgatcttc
    42961 tacaccgagg ttttcgccaa aagtaatttg gatattgtcg atatagcgat tgttccattg
    43021 agcctcaaaa atattattgg caaaacggat agctgagatg ttttggatca tttcttttcc
    43081 taaataatga tcaatcctga aaatatcttg ttctgggaat acttctctga tttgctggtt
    43141 caattcaaaa gcagactggt aatcagaacc gaacggtttt tctatgatca aacgattgaa
    43201 gccatttttt gtcataatat tttgtgattt caaatgagaa acgatcgttc caaaaaattg
    43261 aggtgccata gccaaataat agacacggtt accgtttaat tgatagtgtc catctaatcg
    43321 gtcagacaat tcttttaatg tctgataatg cgttgaatca tttacatcat gggattgata
    43381 gtaaaaatgg cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg cacggattgt
    43441 ttctttgaca acttcacggt agtgctcatc gctccaggga cggcgagcgg ttccgattac
    43501 agcaaaatgc tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg ggtacaattt
    43561 acgctgggct aggtcacctg taccgccgaa tatcgtaaac aatacatttt tttcattaga
    43621 catgtggata cactccttta tattttatcg acaaaaatcg tgctggccgc cttaaagctg
    43681 acggggattt tctttctatc attttggata gtgatcggtc cttcataagc tgcgatgtca
    43741 acgatctcat attcttcgtt gatattcagg tcaatagtga ctaagtaatc caacaattct
    43801 ttttcatcaa gcactcgtgc gatacgcact tttgtaccga caggatagga ctccaatgtt
    43861 tgtcggcgtt cttcatggac aacttcctct tttttaggaa tcgatcctcc atgaggacaa
    43921 tgttcaggat gattcaaata gttttctaaa tgatctgcaa gatgttccga agtcacatgt
    43981 tccaatactt cagcatcatc atggacttca ttccagctat agtctaaatg ttcaactaaa
    44041 aaaacttccc ataagcgatg tttgcgaatc agactgctag ctttttgcag tccgcttgca
    44101 gtcaattgta caccttgata aggggaatgt tctacaagct cttctttcac aagtttagaa
    44161 atcatttcac tgacagatgc agctgagaca tctagactag atacgatttg tttattgttg
    44221 actttcgttg tatctccacc tagttctaaa atgattttca aataatcttc tcgatttggt
    44281 gtcatattcg atcatccttt catggtaaaa aaccatcatt agtaagtgta acaaaaaaaa
    44341 cgttaattga ctatttttct aaataaaaaa caaaggattt ttagacgttt ttacaacaaa
    44401 aaacgaaaga tgatagagaa gactcattct ttcgtttata aaggatatat atatacattg
    44461 ggagggatct atttttcaga taacaactta tatgaagtgt ctgcagattc gtcataaaga
    44521 gtttgttcgg gataagaaaa gataagataa aaaccatact gatcacgtac gactaaacga
    44581 tattcatcta tttctaccac agatgtgcca agagactgac cgttgatcaa aaacgtgtaa
    44641 tcagatgtaa tctgtaattg ttgtttttgc tggctatcac taaagaacca tgtaccttga
    44701 tactttgaga gtccttctga cttttccgct tgttttttct ttttcaagta aataaaactt
    44761 gagagaacgg taatgagtga accaagaaaa tagatcattt tttttatcac caaatcactc
    44821 ctttatgaga aaataatcgc atagacatat tctttcttct ttatttcatt gtaccgaata
    44881 aaagctaaaa tttctctaag actttcgact ggttttttta ctgtttttct acaaattatg
    44941 ataggataca tagtaggaag aaaggtgaca taatttgaag tatcagcaag tggaaatagc
    45001 tttttttatt gaccgcccga atcggtttat agcacattgt atgaaaaaag acggagaaat
    45061 aattattgcg catgtcaaaa atacaggtag aggaaaagag gtcctgatac caggcgctga
    45121 agtggctatt gtatttgctc ctggacctaa acggaaaacc gcctatgatc tgattgctgt
    45181 aaaaaagaag ggtgactggt ttaatatcga tagtcaattg cctaatcggc tggcaattga
    45241 tggtatttta gacggcacga tccttttacc aggattaact ggtccattta cggtatacaa
    45301 gcgtgaagta acttatcttc actccaaatt tgatatttat ttagagacgg tggtcggaca
    45361 aaaagcattt gttgaagtca aaggtatgac acttgaaaat aaagcgattg gtgcttttcc
    45421 tgatgcgccc acactcagag gattgaaaca tataggagaa ttaacgtatg ccgcacaaga
    45481 tggttatgcc gcttatgtct tgtttattgc tcagttcgaa catcttcatc tggcaacgat
    45541 ccatgaagaa atgcaaccag cactagctga tatggttcga catgcgcagc aaagcggagt
    45601 tcaaatcctc gcctataatt gtcaagtatc ggcagatgag gtaactgtca agcaggccat
    45661 tccatttgat gtagatatcc cttttgaaga tcctatacaa tagaaagaag gtaaaataat
    45721 gatcaactta ggtattatcg gaacaaattg gattactcat caatttgtcc aagcagcttt
    45781 aagcacaaaa aaatatgaat tgacagcggt atattctaga catttggaaa aagcacagga
    45841 atttggttct cattatgaag gggatatcga attcgcaaca gatttgaaga cttttttcaa
    45901 tattactcat atggacacca tttatattgc ttctccgaat agtcttcact ttagtcaagc
    45961 taagcaagca attttggcag gaaaaaatgt gatagttgaa aagccagctt tttcgacgcc
    46021 aaaagaaatg gatgaaatca tccatttggc aaatgaacac aatgtgtatt tttttgaagc
    46081 agcaagaaat atccatgaat caggttttaa aaagattgcc gattacttac cattaaaaga
    46141 cgagataatc ggtgcaaatt tcagctatat gaaatattct tcgcgctatg accaagtatt
    46201 agatggagaa gaaccgaata tcttctcgac tcatttctca ggtggtgcat tgatggattt
    46261 aggtgtttac cttgtatatg cagctgttgc ttggtttgga atgccaaaag aagtccatta
    46321 tttcccaaga aagatttcta ctggtgtaga tggtcttgga tgggggatct tacgttatga
    46381 gaattttgat gtatcgatcc agccaggtaa aatcggtgat tcttatttac cgtcagagat
    46441 ttattttgac tcaggaacgc tagtgatgaa tggagtcaat gcaattgaac aagcacaatt
    46501 tcatgatcgc gcgcataaag aaatcattga tttggcggta acagctgcag aaaatccaat
    46561 ggcagatgaa gcagaggatt ttgccaatgt catggctcat ccgaaagatc cagcttgggg
    46621 attgttgtat gaagagtgga tcgagctagc tagaaatgtg aaccaagtga tttatgattt
    46681 aagaaaaaat ggcggcatca aattcgatgc agataatgag gaagattttt aatgaacgaa
    46741 tttgtacaag tatttcccga aaattggcag aaaaaatatg aggataaagg gtttatctca
    46801 ccttctctta ttcaacgagc agtttttcag ccactaagta ataaacaaag tatagtagcg
    46861 gtttctccta caggttcagg taaaacactg gcttatcttt ggcctctact actaaacgtg
    46921 gaaccaggtg aagcaagtgc gttggtcatt tttgcttctt ctcaagaact agctattcaa
    46981 gtagcagatg ttgcacgtga atggggaaaa gacaaagagt tgaaagtcca atctttggtg
    47041 ggcggagcca acgtcaaacg tcagattgag ggtttgaagg aaaaaccaga aatcttgatt
    47101 ggtacaccag gaagaatatt ggaattgatg aaagcaaaaa aaataaaagc acatcaagtg
    47161 aaaacaatgg tatttgatga agcagaccag ctatttgacg ctgggaacag ccaaatcatt
    47221 gatcaaattc ttcatcaagc tccaacagag tatcagctag cttttttttc agcgacagca
    47281 gatcgttccc ttgaaagcat tgaaaaaatc actgggaaaa cgtttgagac aattgacgtg
    47341 acagcagagg acgattctcg aaaaggactg agacattact ttttacgtgt accgatacga
    47401 aagaaagatg actacttgag acgtctcacg catatagaag attttcatgg tctgattttc
    47461 tttaatcaac tgactgaatt aggcgtaatg gaagaaaaat tgctgtatag aggtgtaccg
    47521 gtaggaagtc tggcctctga tcagaacaag ctactccgaa aagcagcgct agaacaattc
    47581 aaaaagaaaa tggtcagagc acttttgacc acagatgtgg cggctagagg tttagatatt
    47641 acaggtcttc cttatgtagt gaatgcagaa gttccgttaa gtgaagaagc ttatttgcat
    47701 cgttcgggaa gaactggtcg aatgggtaat gaaggaatag taattacact cgtacaagat
    47761 aataatctaa gagatttaaa acggctttta cgtccatcaa atataacact tgaagagatt
    47821 tttttatacg gaggtaagct gcaaactgag cagccaacca aagaagatgc agctcctgca
    47881 gtaaataaaa agtatgcgaa aactttttct ggaaaggagc agaacccaga atataacaaa
    47941 gaaaaaataa gaaaaaaagt taaaaataaa tcaaaaaaag agagaaataa aggagcaagg
    48001 cgcaaataag cgccttttta tgctatgatt atgaaaacgg gaacaaaatt ggttgccaga
    48061 ggaggggtta tgtgaaagaa cgtcaagaag agatcttgca gctattgaca gaaaaccaac
    48121 aaggattgac agcaagtgat gtagctgagc gcttaactat cgatcgaagc aatgcaagcc
    48181 gatatttaag tgaactatac aaggcccatc atattgtaaa aaccgctggc cgaccagtcg
    48241 tttactcttt gccaacggaa aagtcaaaat ctgatgaagt gcatgtcgac tcttctacgc
    48301 aagtgacttt tgaaacactc gttggagaaa atgattcttt aaaagtcagc atacagcagg
    48361 caaaagctgc gattctttat ccaccgcggg ggttgcatac gattattttt ggagaaactg
    48421 gtacagggaa gtcgatgttt gctgaatgta tgtatcattt tgcaatcgat tcagagatgc
    48481 tttctgcaga tgcgccattt gtttctttca attgtgcgga ttatgctcaa aatccgcaac
    48541 tgctctttgg acacatcttt gggatcaaaa aaggggctta cacaggagca gctcaagata
    48601 gtcccggtct aatagccaaa gcagacggcg gtatattgtt tttagatgag atccaccgtt
    48661 taccgccgga agggcaggaa atgctgttta ctttcattga taaaggtatt taccgtccgc
    48721 ttggagaaag tgcacaagta catgaagcat ctgttcagat cattggggcg acgactgaat
    48781 cttcagaaag tttcttatct acgttcaatc gacggattcc tatggcaatc accttgccga
    48841 atcttgcttc acgttcactg gatgagcgtt atgaaatcat ctctcttttt atcaaacaag
    48901 aagcgaatcg tttgaatcaa cgtatcgata tcgaaaaaga agcgattctt gccttcatgc
    48961 tttatgatgc ggaaggaaat atcggtcaag tgaaacgaga cttgaaacta gtctgtgcaa
    49021 aatctttttt acattaccgt actcatcatg agaaaaagct aatcattcga aaagaagaac
    49081 tttcccttca agtacaaaaa ggattactga aaatcaagga aataccggaa cgtctcgacc
    49141 gattcatgga cagcaaaagt cagtatttga catttgaacc aggatttgct gatgttgtct
    49201 ggtcacaaga tccagaacga aacatgcagg tctataacga tattgaggaa aaagtcactt
    49261 ctctttccga aaccggtgtg gagaacattg atctagagac cctcatttca aaagatgtgg
    49321 atgcttattt ccagacatat gtcaaagaat tgactaaaag ttcgattccc aaagatcttt
    49381 tgccagatga aatctggcgg ttaaccaatc aattgtatga tgtggctgaa aaacggttag
    49441 accgtattta taatgaaaaa gctcgttttg cttttgcttt gcatctgcaa agtacattgg
    49501 atcgtgtgag agaaggtcat atgatcgtcc acccagattt gaataacgta cggaaaaatt
    49561 ttaagaggga attccaggta gcaatcgatc tttcaacgat cattgaagaa gaagaacaag
    49621 ttgagattcc ttttgatgaa atcggcttta ttagtatgtt tctttctatc aatctaggag
    49681 aaagtgagcc tgttaaagaa aacaaagtag atgttctcat attgatgcac ggcagacaga
    49741 ctgcttcaag catgttggag atggcacaag aactattagg aacaaaagta gggaaagctt
    49801 ttaatatgcc actggttatg gaagttcaaa caatgtatga agaagtgtta caatatgtca
    49861 aaaactatca agagcatttg acgaatggcc tactcttgtt aacagatatg ggatcattaa
    49921 acaccttcag tgatttgatt tatgaagaga ctggcattcg gacgaaagct atcacgatga
    49981 caagtaccat gattgtgata gaagcgatac ggatggcaag tgttggaaga agcttagaag
    50041 atatttatca aaatatccag ctttcctttg aatcgatcgt acgtgatcag ttcaaagcgg
    50101 aaaagtctga aactttacag cgtaaaaaag ccgtaatcgt gacatgcttt actggtgaag
    50161 gagtagctgc caaattatat cagcgtatta tgccagtgat taatcaaaat aaggtagaac
    50221 tgatccagat gcagttttta gaacgtgaat cattcaaaaa acacatcgat gccttattgg
    50281 aagaatatga gatcaaagca attgcgggaa cagttgatgt tgattatcaa aatatcccat
    50341 ttttttcagc ctatgacgtt tttgatgatg aaaaactaaa tattttaaag cgtatagctg
    50401 atgatgagat cccgacagct aaaattgtcg attcacttgc tggatcatta agcgatgtct
    50461 tttctattga tgaactgatc cgttcattgc aaaagacagt tcagcagatc cagaccgatt
    50521 tgtcgattat cgtggaacct ggagtagata ctggaatcgt tatccattta gccttcatgt
    50581 tagacggatt gatcaaacaa gaaagccatc ggcaatttga gaaactgtct attttctcaa
    50641 aaatttatcg tttggaaatg gatatcgttc gggcaaactt gatggcacta gaacgaaaat
    50701 atcaggtaaa aattccagaa gatgatatcg catttcttac acaaatgttt cttgaaaata
    50761 aaataaaaaa atgaacacac taatcatact gtttacacac taaacatctg tgtgcgcact
    50821 ataaaaaaga gctaaaaagc atgaaaacgc tatttcagct ctttttttta ttggcatgct
    50881 tcttgcgtta tataaaagtg taaaaaaata aaaggagtgt taaaaatggc aaaaaaaaca
    50941 atcatgttag tttgttcagc agggatgagt acgagtttat tggtcacaaa aatgcaaaaa
    51001 gcagcagaag aaaaagggat ggaagccgat atttttgcgg tctctgcctc agatgcagat
    51061 aataatttag aatctaagaa tgtcgatgta ttattattag gtcctcaagt acgtttcatg
    51121 aaagctcaat ttgaacaaaa acttgcgcct aaaggtattc cattagatgt catcaatatg
    51181 caagactatg gcatgatgaa cggagaaaaa gttcttgcac aagcagaaaa tctaatgaaa
    51241 taaatcaagt atgcagttgg gggtataaca acgtggaaga tcaacaaaat ctagaagcca
    51301 tcatggggtt aatcatgttt ggcggtaatg caaagagcga tgcaatggaa gcgattgcgg
    51361 ctgctaaaat cggtgatttt gagttagcgg ataggaaaat cgctgatgca gaagaatcac
    51421 ttatacaagc gcatcatgca caaacagaca tgttgacaca agaagcacaa ggaaatcata
    51481 tgcaagtgac attattgact gttcacagtc aagatcactt aatgacatct attgcgttta
    51541 ctgatttagc aaaagaaatc atcgaacttt atcgtcgcgt atacagcgaa taaatacaac
    51601 ttcttttttg aacggaagaa gaaaaatttt tgaacggatt ttttattttg ttataaggag
    51661 aactctcttt atataaaaaa cttataggag gaagtaaaaa atgaacggac tatcaagttg
    51721 gatggagaaa tacatcctgc cggttgccgc aaaaatcgga gcgcaaaaac atttagttgc
    51781 tttgcgtgat gcttttatcg gcatactgcc cgcaacaatg gcgggatcag tcgctgtaat
    51841 gatcaatgca attattcgtg atttaccgca acagtttaac agtacctatg ctgacagttt
    51901 agcagcagac aaaggaatct ttgccgtatt gaatgtcatc atcggaatca atggttttgt
    51961 ctggaacggt acactagcaa tcgccggatt gatctttgcc ttttcttggg ggtataatct
    52021 tgcccgagct tatggagtaa atgatctagc tggggggatc gttggtttag ctaccttgat
    52081 tcaaggtatt tcctttggtc cagcattatc agcagcatta gatgtgaatg ttccttcaaa
    52141 tgtcgcagat gcaatcaata gttctgagat tggtgcaacg attgcagatg gcacactgtc
    52201 agttggatta tggggttggc taaaactaga tcatttgaat ggaaacgctt attttacagt
    52261 tatgatcatg ggagctattt cagtcattat tttttgtaaa ttgatgcttg ccaacattac
    52321 gatcaaaatg cctgattcag taccaccagc agtatctaaa gcatttgcag ccattttacc
    52381 agcgacgatc gctttatacg tcattgctat tatcaattat atcgtaggtc gtgtagctga
    52441 tgggcaactg atcattgatt tggtgcaaaa atatattgca gaaccattcc taggattatc
    52501 tcaagggttg ggagcagtat tgatcgtcac agtatttgtt caagtattct ggttctttgg
    52561 tattcatgga tcaaatgtat tggcaccagt gctagaggga atctggggac aagcacaact
    52621 aatcaatatt gatattttcc aaaaaggtta taacggaaaa actggtacac cagccgtgtt
    52681 agatgcaatc gaagatggaa aagcttatat gtgggtccgc ggatcgttcg atgcttttgc
    52741 atggtttggc ggatcaggcg gaacgatcgt cttactgata gctatcttga ttttctctaa
    52801 acgtgcagat tatctaacag tagctaaatt atccattggg cctggtatct tcaatatcaa
    52861 cgaaccagtg atgtttggtc tgccaatcgt attgaatgca atcatgttta tcccattctt
    52921 gattgctcca gtagtagcaa cagcaattgg atggacagct acttatcttg gcctagtcgc
    52981 tccggtatcg cagcaagtca catgggtagt accgccgttc ttattgtcat tcttagcaac
    53041 aggagcggat tggcgggcac cgatcgtaac attagtatgt atggtcgtga ctttcttgat
    53101 ttgggcacca tttgtcatct ctgcaaacaa aatggaccct gccgcaggcg aacaacaata
    53161 agatgaacgt gaggagcggg acgctttaag tttcgttcct cataatgaat tttcgaggag
    53221 gaactgaaat gattacaatt gccagtgcaa tgcaacaaga tgaaatcaaa gaattgttag
    53281 aaggatacaa tgaagaagga atgactttta catttaaaga aaaacaagga atcaagctgt
    53341 tctttgaaac aagtgcagaa gatccggaag ttgcggctaa aaaagcaaag gaactaatca
    53401 aagcacagcc ttggggaaca gtcctttatt ttcaatcaac agctgtttag aaaatggttt
    53461 aaaaagatag gatggttgag atgataaaaa acggtttatt tttatgtagt attggttctt
    53521 tgatcgtgtt gtatagtttg aatccacatt cgcaaaggta tgatttgtat agtatggtca
    53581 caggtctttt tctaacagga ttaggaatct atttcttcat gaaggggaaa aaaagagaag
    53641 aagcaaagga gaaaaataac taatggaaat cacgaaaaaa ttaaatattc cagcatcgtt
    53701 tttttacgat aaagttatgg actctgtcct tttcgatgta cgtaaagcga ccggcaaatc
    53761 agtaactaga aaacagctga aaaatttgga gtacgtcaaa caattctcgc aaaataaccg
    53821 ggctcgcata aaaatcgaag aagtcattga aaaccagtct tataagtttc gtacatcgac
    53881 aacgaagaat gaattcgtcg ttcattatca aatcaaacca ttggacgatt cgacctgtga
    53941 ggttcattac acagaaaaaa tggagtctta tggcatgctt caaaagttga atgatatgct
    54001 tttaggaaca atcttgatgt attttaaacg gagacgtttc aaaaaaatgc tagatatgat
    54061 cgaagcaacg tattaaatca atgaaacatg gtactattta gttattgaaa aatgaagtaa
    54121 gctagaagga atttttcata ggaagataga tctttcctgc gaatcctcac tagctttttt
    54181 gtaggaaggg gcagcaaaat gacaaacaaa gtaagagagc taggtgtatc ttcgattgag
    54241 gaaatgtttg atttagccac ttatgcattt aatggggcag atacacctga aaggcgagaa
    54301 cgttttcgta cacttgcgga gaattcttgg aattacggat tttttgatga ggaggatcgg
    54361 ctaacaagtc aagtcatggc gacccctttt ccagtgaatt tttacggaca agagtaccta
    54421 atggccggca ttggctatgt tgcttcttat ccagaagcaa ggggacaagg cgggatcaat
    54481 cagatcatgg aaaagatcct tgaagattgc cgtgatcgta aagtaagctt atcttatctg
    54541 gcgccattct cctatccctt ctatcggcgc tatggctatg aacaatcatt tgataaaatc
    54601 agttatcaac taaacagtcg tgatgtgccg tttatcaaaa aaaaatctgg aaaaataaag
    54661 cgtatggatt ttgaagatgc aaaagctgca atcagaagaa tttatgaaca gatgccggaa
    54721 aaccaacgag ctggtctgaa gcgcgaagac tggtggtata cgtataaatt caagcaaaaa
    54781 ggcaattacc agtttgcttt gcattttgat gataatgagg aagcaacggg atatatcgtt
    54841 tatcagcttg caacgtcttc ctttattatt gcggagtggg gatttttgga tcatgatgct
    54901 ttttgcagcc ttgttcgctt cgtaagttct cacaatggtg cttttgaaac atttgaatat
    54961 tcatgcggct acggcggtaa tgatcaaaat tatctattag aaaatccatt tgcttctgta
    55021 atgatcacac cttatatgat ggcgaggatc gtagatattt ctttgttttt ggaaaattat
    55081 ccatttaaaa agagacaagc tgcatttgcc ctacaaatag aagaagatca ttatgcgaaa
    55141 tggaatgaag gaatcgtgga agtaacaatc gaaaatggaa aaaatcatct ctcaaaagta
    55201 gaacaaacaa aactcccaat cgttcgagga agtatccaac atatcacgca gctattatta
    55261 gggtatcgta aagtggaaga gttggtattc caagaaagaa tacaggtgac aaaagaatat
    55321 gaagaaacgt taagctcaat cttgccacag caacagccaa ttcttaatga ctatttttga
    55381 taaaaaacgg gtgatgacaa tatttttgtc accacccttt tgtttttcat ttggcatttg
    55441 aaggaaaatg ttaaagtaaa tcctcttttt tcgtaaattt gaattttttg ttttcttttg
    55501 ttttagggtt ttcttttttg tggtcttgga ttgcttcttt cgtaatcttt tcccgatttt
    55561 ttttagatga atctaagcgt tctgcgtata ctttttcttt tggcttatca ttcattggaa
    55621 aggacctccc tacattattt cattctacct ctagtttatc atctgttaag gactagtgga
    55681 aacgttttgc ctaacttata agctgatttt tttagcagat aagcttgcaa gtaaaaaaca
    55741 aacaagctac aattgattta ttgaaattgt tcctgtagct cagtaggata gagcaatcgc
    55801 ctcctaagcg ataggtcgct ggttcaattc cagtcaggaa cgtataaata acccctgtat
    55861 cctttgcggt acaggggttt gttttgtatt tgtgccaaaa atgtgccaaa ctagttatca
    55921 tttttatata aactatcgaa cacagtattg attttttctt tatcctgatc ttcaagctct
    55981 cgaacaatat gaagataa
//
LOCUS       DAZKOR010000009        55934 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000009 DAZKOR010000000
VERSION     DAZKOR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.009"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..379
                     /locus_tag="ACG8ZM_000466"
     CDS             <1..379
                     /locus_tag="ACG8ZM_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0491457.1"
                     /translation="NFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDDTFWIMLL
                     SHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHEVDMGMPE
                     EEMFSLEVQDLDGNTLEPVWMKM"
     gene            616..1158
                     /locus_tag="ACG8ZM_000467"
     CDS             616..1158
                     /locus_tag="ACG8ZM_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0491458.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1241..1792)
                     /gene="lepB"
                     /locus_tag="ACG8ZM_000468"
     CDS             complement(1241..1792)
                     /gene="lepB"
                     /locus_tag="ACG8ZM_000468"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0491459.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(1944..2171)
                     /locus_tag="ACG8ZM_000469"
     CDS             complement(1944..2171)
                     /locus_tag="ACG8ZM_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0491460.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2168..2686)
                     /gene="msrA"
                     /locus_tag="ACG8ZM_000470"
     CDS             complement(2168..2686)
                     /gene="msrA"
                     /locus_tag="ACG8ZM_000470"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0491461.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(2706..3419)
                     /locus_tag="ACG8ZM_000471"
     CDS             complement(2706..3419)
                     /locus_tag="ACG8ZM_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0491462.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3422..4318)
                     /locus_tag="ACG8ZM_000472"
     CDS             complement(3422..4318)
                     /locus_tag="ACG8ZM_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0491463.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4476..5318)
                     /locus_tag="ACG8ZM_000473"
     CDS             complement(4476..5318)
                     /locus_tag="ACG8ZM_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0491464.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5450..6103
                     /locus_tag="ACG8ZM_000474"
     CDS             5450..6103
                     /locus_tag="ACG8ZM_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0491465.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6166..6693)
                     /locus_tag="ACG8ZM_000475"
     CDS             complement(6166..6693)
                     /locus_tag="ACG8ZM_000475"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0491466.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6713..7660)
                     /locus_tag="ACG8ZM_000476"
     CDS             complement(6713..7660)
                     /locus_tag="ACG8ZM_000476"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0491467.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(7672..9555)
                     /locus_tag="ACG8ZM_000477"
     CDS             complement(7672..9555)
                     /locus_tag="ACG8ZM_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0491468.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(9718..11796)
                     /locus_tag="ACG8ZM_000478"
     CDS             complement(9718..11796)
                     /locus_tag="ACG8ZM_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0491469.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(11809..12084)
                     /locus_tag="ACG8ZM_000479"
     CDS             complement(11809..12084)
                     /locus_tag="ACG8ZM_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491470.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12446..12900)
                     /locus_tag="ACG8ZM_000480"
                     /pseudo
     CDS             complement(12446..12900)
                     /locus_tag="ACG8ZM_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13054..14262)
                     /locus_tag="ACG8ZM_000481"
     CDS             complement(13054..14262)
                     /locus_tag="ACG8ZM_000481"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0491471.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14374..14706)
                     /locus_tag="ACG8ZM_000482"
     CDS             complement(14374..14706)
                     /locus_tag="ACG8ZM_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0491472.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            14854..15729
                     /locus_tag="ACG8ZM_000483"
     CDS             14854..15729
                     /locus_tag="ACG8ZM_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0491473.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(15811..16392)
                     /locus_tag="ACG8ZM_000484"
     CDS             complement(15811..16392)
                     /locus_tag="ACG8ZM_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0491474.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16398..17657)
                     /locus_tag="ACG8ZM_000485"
     CDS             complement(16398..17657)
                     /locus_tag="ACG8ZM_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0491475.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(17659..18705)
                     /locus_tag="ACG8ZM_000486"
     CDS             complement(17659..18705)
                     /locus_tag="ACG8ZM_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491476.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(18940..19215)
                     /locus_tag="ACG8ZM_000487"
     CDS             complement(18940..19215)
                     /locus_tag="ACG8ZM_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0491477.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19458..20768)
                     /gene="der"
                     /locus_tag="ACG8ZM_000488"
     CDS             complement(19458..20768)
                     /gene="der"
                     /locus_tag="ACG8ZM_000488"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0491478.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(20950..22179)
                     /gene="rpsA"
                     /locus_tag="ACG8ZM_000489"
     CDS             complement(20950..22179)
                     /gene="rpsA"
                     /locus_tag="ACG8ZM_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0491479.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(22314..22994)
                     /gene="cmk"
                     /locus_tag="ACG8ZM_000490"
     CDS             complement(22314..22994)
                     /gene="cmk"
                     /locus_tag="ACG8ZM_000490"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0491480.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(23043..23678)
                     /locus_tag="ACG8ZM_000491"
     CDS             complement(23043..23678)
                     /locus_tag="ACG8ZM_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0491481.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(23744..25147)
                     /locus_tag="ACG8ZM_000492"
     CDS             complement(23744..25147)
                     /locus_tag="ACG8ZM_000492"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0491482.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(25134..26165)
                     /locus_tag="ACG8ZM_000493"
     CDS             complement(25134..26165)
                     /locus_tag="ACG8ZM_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0491483.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            26196..26417
                     /locus_tag="ACG8ZM_000494"
     CDS             26196..26417
                     /locus_tag="ACG8ZM_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0491484.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(26582..27319)
                     /locus_tag="ACG8ZM_000495"
     CDS             complement(26582..27319)
                     /locus_tag="ACG8ZM_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0491485.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(27472..28812)
                     /locus_tag="ACG8ZM_000496"
     CDS             complement(27472..28812)
                     /locus_tag="ACG8ZM_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0491486.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(29049..30572)
                     /locus_tag="ACG8ZM_000497"
     CDS             complement(29049..30572)
                     /locus_tag="ACG8ZM_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0491487.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(30950..31561)
                     /locus_tag="ACG8ZM_000498"
     CDS             complement(30950..31561)
                     /locus_tag="ACG8ZM_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0491488.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(31684..31809)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(31891..32607)
                     /locus_tag="ACG8ZM_000499"
     CDS             complement(31891..32607)
                     /locus_tag="ACG8ZM_000499"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0491489.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(32613..33182)
                     /gene="scpB"
                     /locus_tag="ACG8ZM_000500"
     CDS             complement(32613..33182)
                     /gene="scpB"
                     /locus_tag="ACG8ZM_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0491490.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(33166..33936)
                     /locus_tag="ACG8ZM_000501"
     CDS             complement(33166..33936)
                     /locus_tag="ACG8ZM_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0491491.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(33933..34304)
                     /locus_tag="ACG8ZM_000502"
     CDS             complement(33933..34304)
                     /locus_tag="ACG8ZM_000502"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0491492.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(34323..35210)
                     /gene="xerD"
                     /locus_tag="ACG8ZM_000503"
     CDS             complement(34323..35210)
                     /gene="xerD"
                     /locus_tag="ACG8ZM_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0491493.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(35306..35779)
                     /locus_tag="ACG8ZM_000504"
     CDS             complement(35306..35779)
                     /locus_tag="ACG8ZM_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0491494.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(35884..36747)
                     /locus_tag="ACG8ZM_000505"
     CDS             complement(35884..36747)
                     /locus_tag="ACG8ZM_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0491495.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(36943..39042)
                     /locus_tag="ACG8ZM_000506"
     CDS             complement(36943..39042)
                     /locus_tag="ACG8ZM_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0491496.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(39262..40371)
                     /gene="rpoD"
                     /locus_tag="ACG8ZM_000507"
     CDS             complement(39262..40371)
                     /gene="rpoD"
                     /locus_tag="ACG8ZM_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0491497.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(40395..42236)
                     /gene="dnaG"
                     /locus_tag="ACG8ZM_000508"
     CDS             complement(40395..42236)
                     /gene="dnaG"
                     /locus_tag="ACG8ZM_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0491498.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(42418..44733)
                     /locus_tag="ACG8ZM_000509"
     CDS             complement(42418..44733)
                     /locus_tag="ACG8ZM_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0491499.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(44918..45778)
                     /locus_tag="ACG8ZM_000510"
     CDS             complement(44918..45778)
                     /locus_tag="ACG8ZM_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0491500.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(45837..46469)
                     /locus_tag="ACG8ZM_000511"
                     /pseudo
     CDS             complement(45837..46469)
                     /locus_tag="ACG8ZM_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            46636..47397
                     /locus_tag="ACG8ZM_000512"
     CDS             46636..47397
                     /locus_tag="ACG8ZM_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0491501.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            47423..50014
                     /gene="mprF"
                     /locus_tag="ACG8ZM_000513"
     CDS             47423..50014
                     /gene="mprF"
                     /locus_tag="ACG8ZM_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0491502.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(50202..51389)
                     /gene="tuf"
                     /locus_tag="ACG8ZM_000514"
     CDS             complement(50202..51389)
                     /gene="tuf"
                     /locus_tag="ACG8ZM_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0491503.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            51757..52113
                     /locus_tag="ACG8ZM_000515"
     CDS             51757..52113
                     /locus_tag="ACG8ZM_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0491504.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(52354..53358)
                     /locus_tag="ACG8ZM_000516"
     CDS             complement(52354..53358)
                     /locus_tag="ACG8ZM_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0491505.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            53524..54375
                     /locus_tag="ACG8ZM_000517"
     CDS             53524..54375
                     /locus_tag="ACG8ZM_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0491506.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            54392..54637
                     /locus_tag="ACG8ZM_000518"
     CDS             54392..54637
                     /locus_tag="ACG8ZM_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491507.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            54824..55528
                     /locus_tag="ACG8ZM_000519"
     CDS             54824..55528
                     /locus_tag="ACG8ZM_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491508.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        15747 a        11619 c         9601 g        18967 t
ORIGIN      
        1 taattttcct gttgctgatg tcaatcgttc cgttgctttt tatgaaaagc tgggttttaa
       61 aaaaaatgaa gaattttcta ctgaagaagc tggttcaatg gtatgggatg acactttttg
      121 gataatgctt ctctctcatg atttttataa aaaatttata aaagataaag aaatagctga
      181 taataaaaaa acaagtggtg ctttgatttc gtttagtgtt gaaaatgcgg atgaagtaaa
      241 aaaatttgct caaattgcaa aagaaaatgg tggtactttc cacgaagtag atatgggaat
      301 gcctgaagaa gaaatgtttt cattagaagt ccaagatcta gacggaaata cattagaacc
      361 cgtatggatg aagatgtaag cagataacta atctgacgtt ttttataaca aaaaaacaaa
      421 ggcggatgat acgataagta ttctccgcct ctattttttt tattcgtaca atctcttttt
      481 tacagtttat aaaagcagtc ttctcaccct ttttttaatt gttagatgcc agtgtatgta
      541 aatcaccaat gtgtcatgac acattggtga ttttatggta tccttttatt attaactgac
      601 aaaggagaga atattttgaa cactttgatt gcaaaagacg tttttagatt gcccgaaatg
      661 aatgcaagat tgttgtcgac ctctgctctt ttattagccc ttgatgtcat cttatacaag
      721 atagcgattg gtcctgctta ttttcaaatc acattcggat ttttaagtat ggcgcttatg
      781 ggttatctat atggaccgat ctgggctggt ttgcttgaaa tgttgtcgaa cactttaaat
      841 ttcacgatat ttggttcagg aacgtttcaa gctgcttttc ttatcacggc tttccttggt
      901 ggatttatta atggcttatt tttatatcat aaaaagataa acctgccttc cgtcatcgct
      961 gcccaattat tgattatgat cccagtcagc ctttttatca attcttggct gatgtctgtt
     1021 ttgtttggtg cagatttcaa agtaattttt ctaggtagat tcatcaggaa tatgatccaa
     1081 atccctattc aaataatggt tgtttattca tttatcaaaa ctttagaggc tagaaaagtg
     1141 tttaaacgag ctgtttgatt ttacgtacta aggtagtaaa ctgattttgg attaaacaat
     1201 aaagcgtgca gtctcaacag actgcacgct ttattgtttc tcactggatc cattttattt
     1261 catcaagtgg ataatagaca aatcttgctt tacctagaat atcagtttta ttgatcgtgc
     1321 caaacgaacg actatctttt gaaactcttc gattatctcc aagtacaaaa taattatctt
     1381 ccggaagctt ggattgtccc gttagctctt gtagtgtaaa gtccgtcgta taggatgcat
     1441 ttgcatgatc tgatttcagg tttttagtta gataaggttc cttaactact ttcccattga
     1501 tcttcaattg atcattttgg tagctgacat tttctcctgg aagaccgatt actcgtttga
     1561 tataaatcgt gccatctgct aattgaaaaa caacaatatc aaatcgtctg atctccgaaa
     1621 atttttccat cacaaccata tcaccttgct tcaacacgtt ttccattgaa ttgccttcca
     1681 ctggtaccgg gatgaataaa aatccccgca acatgaacgc aaggaataca gaaacaagta
     1741 agtatttgaa tacaagccag aagcgattaa tatagcgttg tttttttgtc aatctctatc
     1801 aatccatccc tctttttttc attctttctt tattataaga aaaatatcaa aaaaatgcga
     1861 ctagatagct gaaataaaaa taattctttc aattaggcta ttggacaaaa aacttttttg
     1921 atcataaagt acaaaactat ctcttatatg tcatgattat tttctaaata tttttcccat
     1981 gcttcgtcaa agatatccat ggacggcaaa tagtcgacat tcagttccag ataattagaa
     2041 atttcttcgt agctagagct gtgcttagga aattggatgt ctttaccggc ttctgtagcg
     2101 aaaacagata aagcatcatg tgtgccaccg cctcgcagtg tcattaaata atgaaaaaag
     2161 cttcgattca cccttcgttc ctccaatttt cttcgataaa ggcagcccgg cgtgcatggt
     2221 ttcttttaaa attttcagga tgctttttat aatactcttg atggtcttct tctgctagat
     2281 aaaatggttg ggctggttcg atttttgtaa cgattggctg ttcgaaacgt ccactttcag
     2341 ctaactgttt cttacttttt tcagcaatga tccgctgttc ttctgtcaaa taaaagatca
     2401 ccgggcgata attgtcaccg cgatcctcga attgtccgaa agcgtcagtt ggatcagttt
     2461 gctgccagta aagttctact aattgttcat atgaaatcaa tgttggatca tagataatct
     2521 ccactgcttc tgtatgtcca gtcgtttggc tttttacttg ttcataagtc ggatctggta
     2581 catgccctcc agtatatcct gacatcactg tatggatacc aggaagcgtg tcgaaaggct
     2641 ggatcataca ccagaagcag cctcctgcaa atattgctcg ttccattatg attccccctc
     2701 taatactact catccgatga ttttttattt tcttttgtct tgctttcagt actgctggtg
     2761 ctagttttgt tggtatttga gttgtttgaa gcatcagagg tatcttttgg caaatataga
     2821 ctaaaccgaa tgtcatcatt tactaagtcg attttatctg ctttaatata catcccgttt
     2881 tgcattcgga attgatccaa cctaagcatg atagtcagat catctgtatt gacttcgatc
     2941 caatctggta atttatagtt tctcttgatc atattcatca catctttgat tggcaaactc
     3001 aatgttccga tagaaagact tctagctttt agttggacat ttccgttttc catcacatac
     3061 ggatcaaaat ataaatagaa atcgatagga aaacctagta cttcgaaagt accattgagt
     3121 agtgcttcat tttccaaata aaatttatat tcaatatcac tatctttttg atactcacta
     3181 aggaaaaagc tgatgatttg attgactttt tctttatttg aattgatcgt taagacagga
     3241 tctccttgtc tttccgtaag agctggtatt tctgggagtt ctggttcccg attagcaaaa
     3301 atacggctgc ctaaaaatac ggtcgaacca agtactaccc caagaaggat aaggaaggcg
     3361 aatttccagt aatttgtttt ctttgttttg tttgaagata cgtcagatct tgttttcatg
     3421 tttattgatc atcctccttc tctaaccatt ggttttgtgt ctcgatcagc tgatctctca
     3481 ctgcatttgc catcaattga taacctaaat tatttggatg gaaatgatct tcttcataga
     3541 gcacattatt tttgatgcca tttagatttt ctgtcgtgct agttgtttga tctgttcctt
     3601 cttctgtgac acctacttcc tgattcaatc cttgataaag caaatcattg attgggataa
     3661 agtagcagtt cggcattttt tttgtcaccg tttctgttcc tttgttccaa ttgttaacaa
     3721 ttgtctgcat gtcagtgatc tcaggaaagt tcaaataaaa tggattgtag atgcctaaaa
     3781 tgtaaatagg tgcggtttta ttgagggatc gaaattcttc caatagttct gtgacatttt
     3841 cctgatagtt tttcagtggc tttttaaagg aattgaccgt taacccgaat aaattatttt
     3901 ggataacttt catcaaatca ttaccgccaa cagtcagtgt gataaaatca gctgttttga
     3961 tattgtttcg aatttcttct tttgttttta ctcgctttaa gatctggtca cttcgttcac
     4021 catttacacc atagttttct gtttcaaccg cggtcaaatt ataacggtct tttagatcat
     4081 ttgcgacaag gggaacgaat cctccttgct tggtttgatc tcctactcct tcagtcagcg
     4141 aatctccgat agctatgtag tgaagttttt cttttttatt tggatcttct ctcacagtag
     4201 ttactggttt cattagtggg ccagctttcg gtatagaagc agctaaacct gcatagatga
     4261 tgatcgcaat taataatggg aacaaaacga ggatcaaatg tcgtttcgtt aatttcattt
     4321 tttcacctct tgtattcgat tggttctaat gttatcaaaa attttatcaa aaaggtagtc
     4381 tttatgaaaa aagaaaacaa ttattttcct aattttataa agaaaaagag aataagctgc
     4441 aagcctttca ggctgagggc ttatcctctt ttcgactaat ctacataata catgattgca
     4501 aacgcgtctt taccagtatg cgtagcgata accggattcg tatgcagtac agggatatgc
     4561 atgtccggga ataattcttg caaaccctct ttgaattctt gcgccaattc ttttgcatcc
     4621 gcatgtgaaa taccgatttg tcgcacattt ggcaattctt tcagttcatt tttcaaatta
     4681 ttaaaccatt tatcgaacgt tttctttcct cgccccttag ttacagggat taattcagaa
     4741 tgggcgaaat tcatcaccac gcgtatgttc aaaacactag acagcaaacc agttgttcgg
     4801 ctgattcttc caccttttac caagttatct agtgtggata ttccgatgta taattttgta
     4861 ttgtcttgta cttttttgac ttcttcaagg attttctcta ctgttttacc ttcttttgct
     4921 aattcagctg ctcgaattac ctgaaaagat aatccttggt cagtggtatc actgtcgata
     4981 accgttactt tcgtttttgt tagattactg gcttgtcttg ctgcttctac tgttccactc
     5041 aagccttttg tcatatggat cgaaatcact tcactgccgt cttctcctag ctcatcatac
     5101 atcgagatga attctccaat tggtggttgg cttgttttgg gcagattttt tgctttcgcc
     5161 atcatttcca taaatctttc gccgtctagt tggtcatcat cgggatatac tgttccgtcg
     5221 atcatgatgg atagcggcat gacacgaatg cctaaagcat cgcgtacatt tttttccatc
     5281 gtacatgaag aatctgttac gatttttaca tttgtcatta ttttctctct ctctttcttt
     5341 aacaagatgc cttattcatg ttaaactaca tgtaagacaa tttctatttt tagtataaca
     5401 gacaaataat gatttttcat taattacacc tagtaaagga gccgttttca tggaaaaaac
     5461 gaaatttaat cgaacgtatg aaatattaaa tgaagtgttt aatgcagtca cccatggaat
     5521 tgggactgga ttgagtatcg cgggactagt ggttctttta gtaaaaggtg cccacttgca
     5581 ttcctccctc catattgtct cttatgctat ctatggatcg atgatgattc ttttattttt
     5641 aacgtctact ttgtttcatt cattgatttt tacaaaagca aaaaaggtgt tccaagtatt
     5701 cgatcatagt tcgatttttc ttttgattgc aggaagttat actccttttt gtttgctgag
     5761 tatccgagga tggctaggtt ggactttatt tgtattaatc tggttgtttg cgattcttgg
     5821 cattatatat aaagcgttga cgttgcataa acgggagaga gtatcaaaag tatctacgat
     5881 catctatatt tttatggggt ggctctgtgt cgtggctgct gttcctcttt atcactctct
     5941 tggaatgact ggtgttattt tgatggtagc aggcgggctt tcttacacga taggcgcatt
     6001 cttttatagc ttgaattcta tccgctatat gcatgttgtt tggcatttgt ttgttatgtt
     6061 aggtgcaggt tttatgtact tctctgtttt attctttact taataaaaaa aataaagcta
     6121 tggatgggac agctctaaat aacagctagt ctatccatag cttttttata tcgtcaattc
     6181 gcctgtcccg ctttttacag gattctcatt acgctgatac gtttcaaatt gatgcggata
     6241 acgatttttt tcatccattt ctccttgact agtattgatc agtgaccaat cagaccagtc
     6301 aacaggtgga aaataagcat ctccatcgaa tacatgatgg atgactgtac ggtataatat
     6361 cgtacaaaat ggcagaaatt cttgataaac agctgaccct ccagcgatga aaaagatacc
     6421 gtctgtttct ttttccagag caagcacgtc ttccacacta tgcatgacat gcacgccttc
     6481 tgctttatag gaagaatctc tcgtcaaaac aatcgtctga cgattcggca acggacggct
     6541 acccatccct tcgaatgttt ttcgtcccat cactagtgta tttgcttctg tcatttgttt
     6601 gaagaatttc agatcatttg gcaaacgcca aggaagacgg tcttcttttc caatcaatcc
     6661 gttttcatcc tgtgcccaaa ttgcagctaa cattttctca tcctttctga ttctaaacag
     6721 cgataggagc tttgatcgcc ggatgtgggt catatccttc taattggata tcttccatct
     6781 caaaatcaaa tactgactgc ttttcctgat tcaaaactaa agtcggaaat gcgcgtattt
     6841 ctcggctcaa ttgttctttc atttgttcga tatgattgat ataaagatga gcatctccta
     6901 atgtgtgaac aaattcgcca accgctaaac ctgtttcatg tgcaattagg tgagtcagca
     6961 acgcataact tgcaatgttg aacggaacgc ctagaaacac atccccactg cgttgataca
     7021 attggcagct tagccgtccc tcttgaacat aaaattgaaa catcgtatga caaggaggca
     7081 aagccatgct aggaacatct tccggattcc atgcagaaac gatcaatctt ctagaatcag
     7141 gagtcttttt gattgcttcg atcacttgtt tgatttgatc gataaagcca ccgtcttttg
     7201 tttcccagtg acgccattga tagccgtaga tgtgtccaag atcgccgtat gtttcagcaa
     7261 aggaatcatc ggttaggatc ttctcgcaaa aaagcttgct ctgcttttta tattcttcat
     7321 tgaattcagg atctgtcaaa catctatggc caaaatcagt catatctggt ccattatagt
     7381 ctggactttt gatgtagcgt tcaaatgccc attcatccca aatatggttt ttattttgta
     7441 atagatagcg gatattcgta tctcctttta aaaaccaaag caattcgctt ttgatcaatc
     7501 caaaaggtac tcgtttcgtt gttaacaaag gaaacccttt ttgcagatca aaccgcattt
     7561 ggtatccgaa taagcttaat gtgcctgtgc ctgttctgtc ttctttacga tgcccttctt
     7621 ccaacaattt tcttcctaag tcaagatacg cttgttccat cctttttcct cctaatcttc
     7681 tacccattca ctcaagtatt cccaccgtgc cattttttct tcaagttgtg cttctgtttc
     7741 agatacgtcg ttttgcaatt cttgtaatcg agtgaaatta tctccttgat gattcatctc
     7801 ttcttggagc aatgagattt tttcttcaag ttctgcaatc tcatcttcaa tcgtttccca
     7861 ttctttttgt tccatgtaag ataatttctt cttttcagca ggttccttgc tctgtatttt
     7921 tggtttcgct ttttcagcag gctgctgggt ctctttttgc ttcagtaaat aactggacat
     7981 gtttccgtaa taaagttctt gttttccgtt tccttggaat actaataact tatctgctac
     8041 cttgtccaag aaataacggt catgggaaac agtgatcaca gcacctggaa aacttctaaa
     8101 ataatcttcc aaaatcgtca atgttgctat gtccaaatca ttggtcggtt catccaataa
     8161 taaaacatta ggttgctgaa tcagcagttt caacaaaaat aaccggcgtt tttctccgcc
     8221 tgaaagtttg cctatcagtg tcccatgcat aaatcttgga aataaaaatc gttcaagtaa
     8281 ttctgctact ccgatttgac tgccatctgc tgtttttgct tcttcagcag cttcttgtaa
     8341 gtaagcaatc atccgtttat ctggtgccat ttcttcattt tcttgtgtat aatacgcaag
     8401 atgaaccgtt tcaccgatag acattgtacc gctatccagg ggaattctgc cagctagaat
     8461 attaagcaat gtcgattttc ccgcaccatt tttaccagta atacctagtc tttcccgaga
     8521 ttgaatcaat aaatctaaat gttctagcag tgtttggtca tttatacggt aactgccatc
     8581 tttgatttcc agtacttttt tccctaaacg ttgtgtcgct aggttcagct ctaaatcatc
     8641 ttcttggttt acttggtaca agttctcctt tagatcttcg aaacggttga tccgtgcttg
     8701 ctgcttggtg ccacgtgcct gtacgccagc cctcatccaa gctaattcct gtttataaag
     8761 ccgcttgcgc ttttcttctt gttctacttc tacacgatca cgttctgctt tttctaagac
     8821 gtaagcttca taatttcctt tatactcgta taattttcca aatgaaagtt cgaagattcg
     8881 gtttgctact ctatctagaa aataacgatc gtgggtaacc atcaatagtg cgccacggta
     8941 ttgtttcaaa tagttttcca accactcgat cgcttcataa tctaaatggt ttgttggttc
     9001 gtctagtaat agtaaatcgg gttcatcaat caacacttgc gccagactaa ctcgtttttt
     9061 ctgaccacct gataaagtac tgattttttt atctaattca ctgattccta atttttgcaa
     9121 aataatcttt gcgttggtat ctgtagtcca agcatcttct ttattcattt gttcttctgc
     9181 tttagcataa cgtttttgca catcagggtc ttctccgttt tcccctagtt cgatcaatgc
     9241 ttcctcatat gctcgaacag tttggatcaa ggggctgttt ccttgaaaga ctgcttggag
     9301 aacagttaat tcatcggaaa attcagctgc ttgagaaaga taaccaatac ggtaatcagt
     9361 tggataaaac actgtttgcc gatcaccgtc gctgctatcg ataccggcaa taatatttaa
     9421 gagacttgtt ttacccgtcc cgttcgtccc aatcaacccg atacggtctt tttcgtggac
     9481 caaaaaagaa atctggtcaa acaaatcttt ttcaccataa gtttttttga ggtcagttac
     9541 ttttaattct ttcaacatgt tccctcctta tcttggatat tgtatcataa aaatagtgaa
     9601 cttcaggaaa atttttttgg tatttgttct ttttgtgttc tcaaaaaaag aacaggaccg
     9661 attcgatcct gttctgatat gttttgtttt ttataaaact tgaaaatttt taccttttta
     9721 ttttattgga ctcgtttttt ttgactttgc tcgaaacgca aatcggaaag aaaagttatc
     9781 agatggtttg ttcatcgtat aagattctaa caaattgata ttcctgcgat agctgttcgc
     9841 agaaaacgtc gtgatttttt cagcaacttc gtattcatca atgatttttc tttcaagata
     9901 ataacctctt agcatttttt tcgtaaacag actatcttgc tggatcatcc acgaagaaat
     9961 caaattcccc gagttcattt ttattccttc aagtttgcgt tgctggataa aaaattgaac
    10021 catcgtttca tcataaatat cagtcaagtt atctaaactt tcaataatca actgagtatt
    10081 tttttgatat gtctctctga tttcttggat atcttcatca ctaataaatg tctgacgatc
    10141 agcatatctt ctttgccaaa acattttcgg atggagaacg attcgcatta acctacggac
    10201 aacaatgaac caaaagccga taaaagacaa aatctggctt gtgatagaat gttcgaatcg
    10261 agaaagaaaa cgggagtaaa aacgataacc attagaagaa agcttgcccg aacgataact
    10321 ttcatccaat ccatcccgtt cgatcgacaa aatcagtgct tgtatttctt gtacttcttg
    10381 cttttcatca tcggttaaat cttccaagta cagatcacgt atccgttctt gataattttc
    10441 tatcactgct tcagtggcta aaaattcttt tgtattacga tcaatttcct ttatttcttt
    10501 tcgcaaaact tcaacgacct cttctaaaat catcaaagca ttcaaatccg tgcttttcgc
    10561 ttcctctgtt tccgtcagca taggcaagac aatgatccct atgaccaaag tcactaagat
    10621 gacacccgcc gtcagaaaca aaagcaaaga ccgctggtaa aacatcatat tattgattga
    10681 aaatggcaaa ataaaaatcg tagccaaact gactgttccc tttacaccgc caaatgttaa
    10741 gatcaagatc tcgttcaact gttttttgaa ctttttactg ccatctttga atacataaaa
    10801 aagactgata gaaatgaatc tgatcacaaa aagcatgaca gagattaata cgataatcgc
    10861 taataaaaga ccgtttggat ataaaccgtc tccccatact ggcgagaaca cttgcgtcaa
    10921 ttcgattcct aaaaatataa atactaatgc attcagtgta aaagcaatag ttgtccatgt
    10981 actgtgggac aagctcgaca attctgcatc aaacacagag attttcctaa aaccagaagc
    11041 ttggagaata cctgctgcca ctgcagcaat gattccagat acatcaaatg cctctgccaa
    11101 tacataggct agaaaaggaa gcaaaagttc gatcagcaga taccctgtga catcttgtgc
    11161 tgatgctttt tcgatcaaat gaattatttt ttgtttgaac cagacaagca aaaagccgat
    11221 caaagctccc ccaatactag aaactactag actcatcccc gcgtttactg ctgaaaaaga
    11281 tccggtgatc agcgcaccaa gagcaaactg gaaggcagta acaccagatg catcattcaa
    11341 aagaccttct ccttccaaaa tatgcatcgc ctttttaggg atattcactc gtccagaaag
    11401 agaactgact gctaccgcat ctgtcggacc taacgctgct ccaaaagcaa agcaggcagc
    11461 aagtgggaca cttggcaaca ggaatgataa cgtcgcacct accccgacaa gggtcaaaat
    11521 gactcctcca aatgccaaga acaaaattgt cccgaaattt ttgagaatcg aagaaatatc
    11581 tgctttttct ccttctctaa ataacaatgg tgcaataatc ataactaaga acaattcagg
    11641 ttcaaaatcg attgattgtc cccactctgt cagacctaat accactccaa ggaaaatttg
    11701 gatcagtgga gctggaatca ttggcaaaat gcggctagct atgttcgata aggtgatcgt
    11761 tactgcaaat acaataacta actcagcaaa ttccatcgtc catccccttt attttttatt
    11821 ttgtgtaagc atttttaaaa attgttcttt tttctttaaa attttggttt cgactacttc
    11881 aacctgtgtt tggttcccct ctaattcaca ctgtacacgt tttgactcca aacgaagcag
    11941 atcatgtttc agacgttcca actgacgata catcaatata tttcttttac gcaacttaca
    12001 aagtatttga cggcggtcat ccatcgaaag ggcgccatag cgatcatgca acagtttttt
    12061 ttgttgttca aaactctctc tcattttcca gatctcctat acacaaactt ccatcattat
    12121 agcaaagttc tttttggatg aaaaatattt cattgtttca tcgtttattg ctagttagaa
    12181 atttccataa atttttcaac gcctcattct attccaaata gtatcagata tcaagtaaaa
    12241 cttaagaaag ataaaagttt ctgacaaaag gatgtttact ttcccattcc tgcctttctt
    12301 gttcttttat cacaacaaaa aaatggggct gcgacaaaaa tcttgtcaca gtcccttatt
    12361 ctgaataaac ggtgtggaag agacagccta tcagtagtaa taagacacat ctctttcaca
    12421 gcctcatttt tgcgtaaaaa tcgatttatt cgaaaaattc cccgtataca tcttctacac
    12481 gttgatctgg attgatcacg atatacaagt tacctgtttt tccctgattt ttgttgattg
    12541 atattgattg tctaatcctt ctgttccttt atctagatat gggaagtaca caagatcagg
    12601 tgtgccattt cggtaaagga tttccattcg ctcgatatct tcgtatttca atgcgcgatt
    12661 aaacatgccc atttccagtc cgcctaggtt gatatcatgt gtcgcaactg cgtccccttc
    12721 atggaagatt tcgattttaa atccagcaca cggatggatt tcaacaaatt cactgccatt
    12781 gattcgtcca aaacttgttg tgatttgttt gatccaaaga tcgccgatat gacggcggtt
    12841 gatcgtccag gtttctccat ttctaaaata aaattttagc gctgtcattt ctttactcat
    12901 taaaaagact cctttatgat gtaatttatt cttgtactac ctcagtttag cacaaattta
    12961 tgtaagtggt cacatcttat gttttttctt ctaatgacgt atctgcccac tttaagtagc
    13021 caacaaagct gagcagatac agtctagtgt tttttaaatc aatttttctt tcgataaaaa
    13081 atccaaaacc tgttttttat cattttttat tctttgttct acgacggctt tttcagctaa
    13141 ttgcaaagct tcactaaccc acttaccagg tttttttcca gtttcttcta gcaagatttt
    13201 accatctact gctaattcgc ttaatgactt gataggtaaa cgatcatagg attctcctac
    13261 ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat tcttctactt gcagtgcatg
    13321 ttttttgcct aagaagtaca attcgaatgg ttccaaagct ccagtttctc gttgtcgtag
    13381 tccttggaca agtgcttgaa cgtctttaat catttggtta gaacacttcc acgatttcaa
    13441 aaaggaacga atctcatttt cttttagatc cagttggctg atcaataatg cccatgcttg
    13501 gctctcttct ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg cttcgccttc
    13561 ttctttcaaa ccgggacagc aaatgtagca ctctgtttca ataaatgcat ggattcccgc
    13621 ggcccgttca tttcccaaga gcatcttgat gaattccaca tttatacgtt ctacagatat
    13681 tttacctaat agctcgtggt tttctttgat ggcgtagaag gtttcttcct ctaatttgaa
    13741 ccctagttga ctgacaaaac gcaacccgcg catcattcgt aaggcatctt catggaaccg
    13801 ctcatgagga ttgcctacag ctcgcaagat ctgttcttct agatcttgca gaccatcgaa
    13861 caaatcgatg atttctccat cttctttcaa agcaaacgca ttgatcgtaa aatctcttct
    13921 tttcaaatct tctgccaacg aacgaacgaa ttcgacatga tctggtctac ggtagtcctg
    13981 ataagttgat tccgtccgaa atgtggtgat ctcatattgt tcatcatttt ctaataccaa
    14041 cacagttcca tgttcgatcc caatatctat cgtttttgga aaaagctgtt tgatttcttc
    14101 tggaaatgcg cttgttgcga tatccacatc atggattggc tggttcagca agatatcacg
    14161 aacacttcca cctacaaaat aagcttcata tcctgcattc tctaattttt tcagaactgg
    14221 aattgccttt tggtattcca gaggtaattg ttttaatttc atttttccaa cctttcctgc
    14281 gtgtttcttg tcctgagata aagactcagt tgcatggtga ctgtttgaca agtccgtttc
    14341 agagtcttca aggccaggac cacttcatct tgtttactcg tccttacgct caaagcgatt
    14401 atggtctctt tgattgaatt tttccatgtt tttttcaaaa acttcagtta agtcgatatt
    14461 caatgaatta gccatgatca tcgtaacgaa taaaacatca ccaagttctt ctgctaccga
    14521 gttaactggt tcagaagcct ttttgctttt ttctccataa gtgtggttga cttctcttgc
    14581 caattcacct acttcttcag ttaatcgagc catctgagct aatggagaga aataccccac
    14641 cttgaattgc tggatatatt catccacttc ctgctgcata ctgtataaag aacgcttttt
    14701 atccatcttt ttccctccaa aactatctta ccaaaacttt aacgcaaatc ctagataaga
    14761 gcaattgaga aaatcgaatt tccatgctac atttaggaag gaatttctaa atggaaattc
    14821 tgtgaaaata cataattgaa agctggaatt catatggtca aacgaaaaac agtaaaagaa
    14881 gttcttttta tcattatagg aacaagtatt tatgcatttg gtcttgttta cttgaacatc
    14941 gctaatcatt tagcagaagg gggagtctca gggatcacct tgatcttaag agctttattt
    15001 ggtattgatc cagcttatac gactttatta atcaacatcc cattgatttt actaggagga
    15061 aagatcctcg gtaaacgttc cttggcctat actgttctag gtacactttc tttgtcattc
    15121 tttttatgga tctggcaaag ggttccacta gaaatcaatt tacagcatga tctactgatc
    15181 gtttctctac ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta ccgcatgggt
    15241 ggaacaacag gcggcagtga tattatcgct cggattttag aacaaaatta cggcatcact
    15301 atggggcgtt ccctattggc atttgatatc atcgtcttac ttgcttcatt aacctatatc
    15361 gacttgaaac gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg tgtgattgat
    15421 tcgatcttag atggcggata ttctgcaaaa ggtctacttg tcgtctccaa caaaagcgaa
    15481 gagattgcac cactactgat gaccggtctg caaagaggtg tgactttcct ttctggagaa
    15541 ggcgcattct ctggtaattc aaaaaaaatc atctatatgg tcgttagctc tagtgaatta
    15601 actgaagcta agaggatcgt ccacgagatt gacgaaaaag ctttcctttc aatcatcaat
    15661 gtgcatgaag tggaagggca aggctttact tatttgaagc cacagacaag attactgaaa
    15721 aaactataag aaaaaagaca aagataggct atgaggaaag attccacatc cagctgacct
    15781 ttgtcttttt tagtactata ctatttttaa ctaattgtta tgttcactta cccggttact
    15841 tttcttttgt gtctgcaaaa ggatctttcg gttaacttca tcagataatt tcaaaaaagc
    15901 atccctatct ccatcttcta gtgcttgatc gatttgagca taaagcattt ttagttttgc
    15961 ctcttcttct ttttcttgaa aatatcgttc aattaattca gtatcttctt cactgactga
    16021 agaatcccaa gaagaaaatg gattgtcttc taagatagat agatattctc ttgtctgcca
    16081 agcattttcg aatatacatt ccacatataa aggctttttc caattcaaac ggatttcatg
    16141 aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa aacaatttca tgggttcccc
    16201 ttcaacagat agatcactga tctgcagtcc tcgatccgca aaattcgccc cttcaacaaa
    16261 gtggacattg cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc aaaaaacttc
    16321 tcttcggcta aaagatacat gattgaccat ccacgttaaa aaattcttct tttcccgtac
    16381 atcaattagc atttttctca cctctctgtt aggtcatcta ataatgacag tgcctccata
    16441 tctcctggtt caagtgttaa ataataagtc aataattctt gcgctctttt cagctgccct
    16501 tcttctctta agaaaaaggc atactctttc aagaaatctg gttcatgtga tagttcatga
    16561 taagcctgtt catagtgtac agctgcaacg gtaaagtctt ctaattcatt ataagcatgg
    16621 gctaggttcc attctgcata aggattagca gtttcttcca tctgattgat cacttgtatc
    16681 acatcttcat atctttcatc atccaagtaa agattactta aagtaagtaa tgtttcatct
    16741 tgtttgtctc ctacttctaa agctttcagt aaaagttctt ctgctcgctg tctatcatgc
    16801 agacggaaag cattttccga ggccaggtga taaagttcga cttggtaagg attttctttg
    16861 attccttctt ctaacactgt ttgtgcttct tcaatcattt cttcttcttg aagagcttgt
    16921 cctagatata gatataacga ttgataatgc ggattaactt ctcgaagttc ttccagatag
    16981 ttgattgcct gctgattttc tttcaattga agattcgtaa aagctagctg gaacagtcga
    17041 tcatctgtcc tttcttcttt cagtgcttct tctaaaggta cgatagctgc ttcgaattct
    17101 ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg agattcctga aatttcattt
    17161 atcccgcttg ccaacaatgt ttcataaacc actgctgcct ctttgaaacg atcaactgaa
    17221 aaatacaatt cagctagcgc aaactggatc aatggttcat caggaagcaa cccagctgct
    17281 tctttcaatt ttgcttcact cacttcaggg attccgataa cttggtacaa atcagctatc
    17341 gccaataagc tttgcggata gtaatcactt tgcttatcca ctttttccag gtattcaaaa
    17401 gctaagtcga tctcattatc ttcgattgca atttctgcta acgggatgtt cagtccgtca
    17461 ttttctgggt attggttgat cagttgttca aaaatctgtt ttgcttcgat caaaaagcca
    17521 atagccagca attcttcgcc tagttcagct aatatatctg ctgggtcttc ttttaatgct
    17581 ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg ataaagcttg caacattttt
    17641 tcgctatttg tattcatttc aattccctct tatctcactg acgatttgtt gaaataccgt
    17701 cattgttctc aataattctt tttcgtttgg atgttggtat ccgtctatta tacctatttt
    17761 ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa agaacttttt ccaaatactc
    17821 catctttgaa atctgttcca gccagtggat tggatagcca agcaagtcca gccacacaaa
    17881 aaagtttttt agttggaaat caaaaggata tgcttggaga ttccacacga aatgaaacat
    17941 cattcctaaa aattttttca tactagcaga aagtagatgg ccattttcgg tcagataaaa
    18001 agcttgttca aacagtttac cataatttga caggttttct gcctctaatt gataaaaata
    18061 gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt tatttggata
    18121 gtttttatac agattttgca aaaaacgatg atcacaaatc attccgcaac agataaaagt
    18181 caaaaagtca atctgctttc cgtccacctg ccgctcgctg attgtttgat cataaacgat
    18241 gcgttctggc agattgatgg tttgcagcac tacacgattt ggtagctgca aaatcgtccg
    18301 ctgcggtgtt aatgctcggc ttagagaacg gatcgataca gggatcatcc atagtgatga
    18361 atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc atgatccctt catttccaaa
    18421 tccgatgata aacatggatt gattttcggg atagcgtttg acgaaatcaa gcaggttctt
    18481 taattctgtt aaatgattgc attgagtgtt ggcacaaata taccagtcat tgtttgcttg
    18541 atttgcaaat aacgggttga tcttatctgc aaacaaatca taatagcgct gattcgttaa
    18601 aaataagatt ggctgatcaa tggaaagttc tcttagctga ctgactagcg tttctccgta
    18661 aaacactttc gtcaacgcat cttctacttg ataatcaagt tccatacgca tccctcctct
    18721 tcttattttt attttaccat tagtaagcaa attctagaaa agataagagc aaagctcacc
    18781 tcataacttg caaataaata aaaattaaat aaaatacatg tgttttctat gtatgacata
    18841 cggattcgga atctctgtct cttatttatt ccttttctgc taggaaataa aaagaaaagc
    18901 acttgtagcc ctagagccac aagcgctttt tcaactgtct tatttaacag cgtcttttaa
    18961 tgctttacct ggtttgaatg caggtacttt gctagctgga atttcgattt cttcaccagt
    19021 ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact tcaaagttac caaaaccgat
    19081 taattgaact ttttcgccgt tagctaaagc atcttgaata gttgaaaata cagcatctac
    19141 tgctgcagtt gcatcttttt tagttaaatc agtagctgaa gcaacttttt cgattaattc
    19201 tgctttgttt gccatggata gattcacctc ctccacaaga gatatgatag aaatatcatt
    19261 tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa atattgatta acttcaatat
    19321 tctgttatca cgatatcata tgaaccgctt aatagcaagg tttttaccaa ttttcacatg
    19381 ctttttttag tcatagaaac gtaacaaacg aaactgtctt tttcttttga aaaaaaaacg
    19441 tcgatatggt aaaatgccta ttttctccta cgagggataa ttcggatgga cgtcccttca
    19501 aaagtaaatg cttttctgat ttgattttct aagaaacgtt cataagaaaa atgcatcaat
    19561 tcctcttcat tgacaaaaac gacaaatgta ggtggtttga cagcgacttg tgtagcataa
    19621 aaaatcttca ggcgttttcc tttatctgtt ggtgttggat tgattgctac agcatccata
    19681 atgatatcat tcaacacagc cgaagagata cgaagattct ggttcatact tacttgttcg
    19741 atcatttcag gcagacgttc caaccgttgt ttagtcactg cagaaacaaa aacgattgga
    19801 gcgtaatcaa gataccggaa ttcatcacga atctcttgtt cgaaatcacg cattgtattg
    19861 gtttctttct ttacaagatc ccatttattc acgacgataa taattccacg accagcttca
    19921 tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc cttcttcagc attcagtacc
    19981 atcaaaacga tatcagaacg gtcaatggca cgcattgcac gcatcacgct gtatttttca
    20041 gtattttcgt atacttttcc gcgtttacgc ataccagctg tatctatcat taagaatttc
    20101 tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc gggtcgttcc ttcaatatcc
    20161 gaaacaatta cacgttcttc tcctaaaatc gcattgatca aagaggattt accaacattt
    20221 gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct cttcttcagt ctcttcagag
    20281 aaatacttca ctgcttcatc taaaatatca ccaatcccta gtccatggct tcctgaaaca
    20341 ggataaggat cgcctagccc taaggaataa aattcataga tatcgtttct catttctgga
    20401 ttatctactt tattgactgc taaaatcact ggtttattac ttctgtataa aattttcgca
    20461 accatctcat ctgcgtctgt cacgccttcg cgtccgctga ctacacaaat gattacatct
    20521 gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat ccataaaagg ttcatcgcca
    20581 agatcaattc cgcctgtgtc gatcacgctg aattctcgac ctaaccattc accttttgca
    20641 taaatacgat cacgagttac tccaggagta tcttcgacaa tcgagatacg ctcaccagcg
    20701 atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc caacgattgc aattgttgga
    20761 tttgccatat tatttcctcc tcacaagttt ccaatctcac ttagtttaac gaatgcattg
    20821 ccaactttca agcatagatt gtcaattaaa cgaagtattt ttattcctta ttgaaatact
    20881 aatcgctttt caaaagaaaa actgagacaa aagcagctgc tttcatctca gtcacagata
    20941 caaaatcaat tatttttcag aatcatcggt tgctttttct tctacttctt ctttcaacgc
    21001 atcacctaaa atgtctccca tagtaaatcc agtattttct tctggtaatt cataagactc
    21061 aacatttttt ggttcttctt ctgaagctgg tttttcttct aaagctttga tactcaatgc
    21121 aatacgatgt tcttctggat ggatctctaa tactttcact ttcacttcgt caccttcatg
    21181 aagcacttca tgtggtgtcg caatatgttt atgtgaaatt tgagagatat ggactagccc
    21241 ttctactcca gggaacactt caacaaaggc accgaagctt gtcaaacgtt taactgttcc
    21301 gtctaatact gtgccaactg gagcttttgt atcgatttct gaccaaggtc caggctgtgt
    21361 gtctttgatt gacaaggaaa cacgttcttg ttcaggattg atagacaaca ctttgacatt
    21421 tacatcatca ttgacagcca atacatcact tggttttgct acatgactat gtgaaatctc
    21481 tgagacatgg actagaccgt caattccgcc taggtcgaca aatgcgccaa aatcagtaag
    21541 acgtgcaaca cgtccactga gaacatctcc ctcatggata gttgaaagaa tttctttttt
    21601 cttttcttct ctttcgtttt ccactaccgc tttatgcgat aagatcaaac gattttctga
    21661 tggttcgatt tcgatgattt taaagctcaa tgttttgcct ttatagtctg aaaagtctgc
    21721 aacaaaatga tcttccacca ttgatgcagg gacaaaacca cgtacaccga cgtcaacgac
    21781 taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg accttgccct cttggaaatc
    21841 tctttcgata tcttcccaaa cttttttagc atctaaacgg cgttttgaaa gtagatagct
    21901 gccgttttct ttatctttgc cgatcgtact gataacgact aaatctaata catcaccgac
    21961 ttttactact tcgctgatat cttctaccgg caaagttgat aattcttttg ccggaacgac
    22021 tccttcaact ccagcccctt cgattccgac aatcacttgt ttgtcttcaa tgactaaaac
    22081 ttcgccctta acaacatcgc caacttttac ttcttgtacg cttttcattg cgtcttccat
    22141 tgactggttg ttctcttgat tttgatcaaa ttctgtcata acacttgtcc tcctaaccaa
    22201 cattctgccg taagtacggt actgttttta gtttaattga ttattcaaaa aaaataaagc
    22261 gatttcgttc gttttccttg aaaaaacaat tgtttttcat tttttttctt aatttaaaaa
    22321 agaagataac cttttttcaa aacaacagct tttattgctg caacgacttc tggaatcgat
    22381 ttgccagtcg tatcgatacg aacagcatcc tctgcttgct tcagcggaga gacgtccctt
    22441 gttgaatcat aatggtctct ttcctgtatc tctttcgtca gtgtttcaag atcagtcgga
    22501 attccttttt cttggttctc tttgaaacgt ctttctgcac gctcacttac gcttgctact
    22561 aaaaagattt tcacttctgc attcggtaat acagctgtcc cgatgtctct tccgtccatg
    22621 acaacgccac cgcgttcccc tatttttcgt tggacagcaa ctaattcttc tctgactttt
    22681 ccatgtgcag agacttcaga aacagctttt gtgacatctg gttgacggat ttctaaagtc
    22741 acatcttttg atgctatgaa tacatgctgt ccatcttctt gttgcgaaaa agagatagga
    22801 tactttttga tcagcgaaac tagcccttct tcatctgaaa aagatacatt gttcgtaata
    22861 gctaaataag ttactgcgcg atacatggca ccggtatctg tataaatata attcaattct
    22921 tttgctaata tcttggcaac ggtacttttc ccagatgaag caggcccgtc gattgcaata
    22981 ctgatttgtg tcatgatata actccttttt ctcagaaaaa aagccgcaaa gcgcggctat
    23041 tattatttaa ttcttaattc ttgaccaggg taaagcataa agttgtttgg atctaagcca
    23101 ttcaattcaa acaattgatc agtggtgatc cctgcacgct cagccacttg ttttggacct
    23161 tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg atgaagaagg tgttgtttct
    23221 gctgcattgc tgcttgaatc ccctgcttga taagttgcct cacttgagga agtcggtgtt
    23281 gtctctgctg caatactgct ttctgacgtt acacctgtat cttgagacac agtagattct
    23341 tccgcgattg tactagattc tactgttgaa gtctcagttg actcctcttt tgttgtactt
    23401 gtagaacttt ctactactga tgtcgaagtt tcagaagaag ttgctgctgt tgcattgttg
    23461 cttgatccat ttctgatcca gaaataagca cctgctggaa tagcgataca taagaccagt
    23521 aaaatcaaaa atagcgaaag aaataatgta tttcctttct tttgttgtct ctgagaactt
    23581 cttgatgaaa cttcatcatc tgtatcgtag attggttgtt cccatggttc ttgtgtttcg
    23641 ttattttctg tagattgatg tctatctttt ctgctcactg ttttacctcc taaactctat
    23701 cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa aaatcaatgt tccatgtttt
    23761 ttttaaataa atttagtaaa atcgttcccc aatgaagttg ttcagtctta gaaaaaagct
    23821 tcctctttgg cagaagttct gcttttgctc catcaatatc acaacaattc tgcggctttt
    23881 cggagagttt ttcgccaaaa taagctaaga tgaattttct gcggcagttt tcttcattaa
    23941 tatagcgaag catctgctgc aggcggaatt ctttttcttt ttcctgacgt ttgagccgtt
    24001 ccacccaatt ttctggtttt tcagactgct ggatcagctc catccatttt ttttgcagct
    24061 catcaaaggg agcttgctct gtggcatatt ccaaataaag ctcaaaactt tgtcgctgtt
    24121 ctctagaaag ttgattaaag aaataatgga tacgttcatc cccactttga taaaaaagaa
    24181 ttgctgcact ttcttcctga tctctgcctg ctcgaccgat ttcttgtaca tagttttcta
    24241 gactgtctgg caaatcataa tggaccacat aacggatatc agatttatcg atccccatac
    24301 caaatgcatt cgtagcaatg agaaactgca gctgattttt tacaaactgc tgttgaagtt
    24361 gtcttctttg agctgcatca agaccaccgt gatagtatcc aactgtaaaa cgttccctga
    24421 ataaatgata caatcgttcg acttcttttt ttgttgcaca ataaatgatt gccgctcctt
    24481 gtgctctttc catgaagtgt tcaagttctt gttctttttg ctgcgttttc cgaacaaaaa
    24541 gcgagatatt ttgccgattg actgattgtc tgatctcttt tggtggtcga tcaaatagta
    24601 catgacgtat atctcttgct actaaatctg ttgctgtcgc cgttagtgcc agtgtgaccg
    24661 ggtttcctaa aagctttcgg atttttccta actgttgata ttctggacgg aaatcaactc
    24721 cccattgaga cacgcaatga gcctcatcta ctacatataa cgcaatattt tgttttttca
    24781 gatgctctaa aagagaagga ttagtcaaca tttcgggact caaaaacaaa aatttatatt
    24841 gcgatagatg acggaggacg tatcttcttt cagattgcga aagtaaacta ttgaaagcag
    24901 ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg atcttccatt agagaaagca
    24961 gcggtgacac gatgatgacc agaccttcca ttaaataccc cgtcaattga tagcaaaggc
    25021 tttttcccgt tcctgtaggc aaaatagcga gagtatcttc tccatcaagc aacgcctgaa
    25081 taatttcttc ttggccggga cgaaatgaat caaatccaaa atattttttt aattcattta
    25141 ttaacattct gtatcgcttc ctttctcaat agatagattt gatacaaacg aaattctcca
    25201 taatctaatt gtccatctac attccattcg ctaaagcgcc actctcgcac ggaatcctta
    25261 tttggttcca aacgttcgat tgtttctgca gagagaatcc actcgtatgg aaaatcatca
    25321 aaaaacaagg cccactcaat aagatgatct gttactgttc ctctttttaa atgacggatt
    25381 gccatgatct ggtcaattgt ctgaccattc ataaacatct gcttggtgat catcatgctt
    25441 tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt gccaaagcga atctggccgt
    25501 ttttcgattt gtgctaacaa aaggtggata catctgcttt gttgcagata aaccgctgac
    25561 tcatcattgt tttttgccag ctgataagga agcagccctg ttcgctcatt tccagataat
    25621 tggttagcta aaaaatctgc tgcttcttct ggtatctccg aaaacagctg caccaaactc
    25681 tcatacgcag tatcaatcaa aatttcacgt ggcaattggc tttcactaag ccattttttt
    25741 agttggaaag tataaaaagg acttgtctct gctggaagat agtcttgaat acccgttgcc
    25801 agattagaaa ttacttgaac agcaaattta atgagccgcc aactagtttc ccctgttctg
    25861 ccatagcggt caaaacgcaa accagtatgt tctatttgat gttcggacaa catatcagcc
    25921 cctgcaggcg ttagctttgc ttcattttca tttatttcga tccaaccttg ctgcgcaatt
    25981 ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg gaaaactgtt atggataaac
    26041 aaaagttcgt gggtaaatcc ataaataagt actgaagtcg ttcgttttcc cacaaggagt
    26101 tggtaaagag aactaatccg cagcttgtgt cctgatgaaa atagcgataa aataaaaaca
    26161 atcatattat tctccttaag ttaggatggt tagaaatgtc actactatgt aaacttgtac
    26221 ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc gccagatatt ttcgattatg
    26281 atgaagaagg aatcgtttta tttgcccaag aaccagaggc tcatcaacag ttcgtgatgc
    26341 cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg tccagttcga gcgattttat
    26401 tagaaaataa aatataaata taaacgaaac tttaaaaacg tacactaagt tctctaaact
    26461 tgaaaataag ccgggatctg tttctcttgt tccgtgaaat cccgacttat tttgaaagat
    26521 tcatttgcta tctatagtta attgactagt tttttcacgg accctatagg acccatttat
    26581 attatttcaa ctttttcagc cagttttcca atgtattgac gatcacagtt tgctggtctg
    26641 cgttagaaat ttctgcctct ccatctcctt tttgctcacc atagctgcca aaacctccat
    26701 gatttccgcc attgattgat acatactctg ttctttccgg taaatactgc ttagcttctt
    26761 gataagcttc ctgattcaat actttatctt ttgtcgccgt gatcgataaa acaggaacag
    26821 ctgattctgt caatgttcct tttttgtcgg gataacttgc catgaagaat acccctttta
    26881 aatgtttgtc tgaatgattc ttattgacat aacgacttgc cattacgcca cctaatgaat
    26941 ggccaccgat cacataatcg gaatttggaa aatcttcttt gaccttatcc gctttgtttc
    27001 cgcccatcac tgctagatca agtggcattt tgacaatagc gactgaaaag ccgttttttg
    27061 ctaaatcttg cgcccaaata ctataacttt ctggctctac gagagcacca ggataaaaaa
    27121 taagtaatgg agactttcca tgattctttt tctctccttc aaaaaataag taatcttctt
    27181 tgttttttcc ttcttttacc aacacttgcg cttctgacga tggttcgtac gtattttgct
    27241 gtatatagta ataaccggct ccaccgagta atcctaaaat gatgactaag ccaattagta
    27301 ttcgtctaaa tattttcact ttctcccacc ttttctatct gagtgacccc tttttcttaa
    27361 tcaagtatga ttatacctta aaaaaataag agagaaatgc aaactttcca gcatcaaata
    27421 aaaaaagacg gaatatccat ttgaatatcc gccctttttg tatgtttatt tttatttagc
    27481 acataaacga tacacagctt ctgcataaat ttccattgct ttatacatac tacttaattc
    27541 ccatcgttca ttcgcctggt gcataaagtc aggagtatca gggaacatcg caccgaatgc
    27601 cacacactgg ttcatcgtac gagcaaaagt agcgccgcct gatacaaacg gttcagtcat
    27661 gtctccagta atatcgcgat atgtccccaa tagatttttc actaactcac tgtctagtgg
    27721 cacgtataat gaatcaagga agtcaaattc ttcataagtc aacccatatt tttctgctgt
    27781 tttagttaat ttagctacta aatcatcttt cttgaatgta acagggattc tcatatctac
    27841 accgattttt gtttgttctg gtgtgatttc caaacttgca aagttcatcg ttagctcgcc
    27901 ggattgttca tcttctgttt tgcccacgac agcttcacca gttgcatttt cttgaactaa
    27961 ttttcctaag aaattgattg ggccaaaatc aaatacttct gaaagagcaa ttgccagacg
    28021 agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg atacttttac caagaacgac
    28081 gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt ttcaatgctt cttttacttc
    28141 agaaagcttt tctccagagt aaggagctgc atcaggaaca acattcaacg ctcctccagc
    28201 ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa gcttgcaata agcctttttc
    28261 tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct tgcgtaattc cttcttcttt
    28321 ttcgttatat ttttctaaac aacgccataa attttcttca tctgtaccaa agataaaacg
    28381 aatacgtgtc ttgaattcca cgccagcatc aatcaaagct ttgacggcat acattgccgc
    28441 aatagaagga cctttgtcat cttgggatcc gcgaccaact aaccagccgt cttttatagt
    28501 tggatcgaat ggttttgttt cccaattatt ttcgtcccct gctggaacaa cgtccatatg
    28561 acacaagata ccgaataatt catctccaga gccaatctct gaataaccgt aatagccttc
    28621 tggatcttta aatgttctaa atcctagctt ttcactgatt tccaatactt tatccaaagc
    28681 ttcaatcact tttttaccga agggatgtcc ttgtcctgaa tctgcttcat ctagtacgga
    28741 aggaattcgg atcaattcgc tcaatgattc taatgcttgt tcatgatgtt gttctgtcac
    28801 aaattttttc atgatattat cctcctaata tgttaaaaag ggtcatgtca aattcctccg
    28861 acacaacctc ttcttctcaa ataatgacgt tttaagcctg ctccatcagc aagcagaatg
    28921 ttcttaacaa atgatgaaag aaaaaacact cactgaatgg agctattgac ttatccacgt
    28981 gcttatcgat agccgtttat cggtttcttc attcttatga tagaccttgt acctgtattt
    29041 gtctaggttt agaacattgc tgctacacct aggatcacac agatggctac aaataatatc
    29101 gcaattaatt taccggtaaa tttccaccac actgaaacat cgaaacgagc aattgctaca
    29161 gcacccatta cgatagcaga tgttggtgta attaagttta ctaaaccaga tgcagattga
    29221 taagcagtga ttactaaatc cttaccgacg ccggcaaatt cacccatagg tcccatgatt
    29281 cccattgttg cagcagctaa gccggaagtt gaaggaatca agaatgacat agggatatag
    29341 aaaataaatg taataatgat gaatacactt gaagataatg aacttaatcc ttgttcgccc
    29401 caatgtaaga tggtgtctgt gattaatcca ttgttcataa ccacttggat accacgagca
    29461 acagctacta cgattgctac accgatcaaa tcagaagcac cagctaaaaa ttcagagaca
    29521 aattcagact ctttcatgcc ataagtgatt gctactacga tggacattac taagaataac
    29581 atagtaattt ctgggaagta ccagtcacct aatggaagca tgcttttacc tagaaccgta
    29641 cctagtaccg gaaggtttgt taaccagttt gtcaatgatt caaaaatcgt gaaattcgca
    29701 ttcaaggttg tccaaggaat caaactgatg atcatgatac caaaagtaat aaagaacaat
    29761 acattaacat gtttttgttt tttcgtgatt acttcttgac gaccgattgc ttcaatatcg
    29821 aaatgcttca tatcttcttt acgttggttg tatactaatg attttgttgg atctttttcg
    29881 atctttgatg cataacgata cacatataca atagaaatag ctaacaaaat aacgaacatc
    29941 aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa cagcttgtga agctacgcct
    30001 gttgcaaaag gatttaccgt tgaagctaga cagcctactt gagaccctac taatacgatt
    30061 gctacagcta ctactgtatc aaagcctaca gccatcatga ctgggatcag taaagcatag
    30121 aatggaatcg tttcttctgc cattccgtaa gtagagccgc ctaatgcaaa taacagcatc
    30181 aatactggaa taagcatttt ttcttttcct ttgaatttct tcacaactga agcaatcccg
    30241 gcatctagtg cccctgtttt gttgataata cctaaaaatc caccaacaac caaaatgaag
    30301 aaagagattg ggattgcagc aggtgtttct ttagttagct cccccgctgg tacacctagc
    30361 atcccattga ttggcgccat gaaaatatcc cacagccctt gaggattttg ttgtactcgt
    30421 tcatatgatc cagcgacgat gttgcctgca tcgtctacac tatagtgccc agctggtata
    30481 aaccaagtaa gtacagcgat tactgcaata ataataaata agaccgtata cgctgaaggc
    30541 atttgaaatt ttttcttttt tgtttgttcc atcttcttct tcctttcttt tccgttagag
    30601 acaacaattt tggctctaat gttattttat caaaaagagt atcagaacaa aaataataac
    30661 acgcattttg taaaactttt gttcaaaaag ttcgtttttc cctattccct cctctttttt
    30721 caggttttta aagctttctt ctttttcatt gtagcgtttc ctttttttta taaacaaaca
    30781 ttttcgtctt ttaaaaggag aagaatttca catttgcaaa gaattgattg atttttatgc
    30841 ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt tacattattg catgtcttgt
    30901 atttttttcc aaaaaaaaag accaagaaat ttcttgatct ttttgatatt tatttgactg
    30961 ctgtatgacg ttgttcgaac tgatgttgtt ttcgacttaa ccatggaaga agttgcgcat
    31021 gcagcactaa aaagacagca ctcacgatga atcctttgat caagttgaaa ggaacgatcc
    31081 caatcagtac atagtttgct aaagacatgc ccagcatctg tgttgcgttc agccctaaaa
    31141 acattaagta aagaggcgta ataacgaaat aatttgcgat actcataaaa atagttagag
    31201 cgatggttcc tgtaatgaca cctaaaaatt tgtttcttcc tttatgctgt ttctttacac
    31261 taaagaaata ataaatcggg aatgtaaaga tcgttgttga taagaaacta gcagtgtctc
    31321 ctaccaaatt atccggagaa aaacctgtag tcagcagatg taatactgaa cgcaagaacg
    31381 ctgttgcaac tccggccgct ggaccgaata agaacatgct gatcaatacc gagatatcac
    31441 taaaatcgat cttcaaaaaa ctaaaagccg gcatgatcgg aaaagcaaaa tactgtaaaa
    31501 ctaatgcgat cgcagcaaac atcgctactg caaccatttt ctgtacacgg gtgttcttca
    31561 tttgaaatcc tcctgttagg actcctcttc agcgaacttt ttgacgtttc tctccaaatg
    31621 aaaaaactct attttccagg gaaaatagag ttagagttat ttgatcaagc gtcgtcaaat
    31681 aagctctatc ttctttatcc agactatact gtcggtatcg gaatttcacc gattcagcta
    31741 tttttttgca aaataggtcg tggactataa ccaccggtcg ggaatttcac cctgcccctg
    31801 aagacgaacc ttatataatt tttgtttaca tattcattat acatatctat tcaaaaaacg
    31861 caacatctaa cacttataaa aagtaagtgt tcattttgag tggttcagca aactgctgac
    31921 ttcttttttg ttcagttcac ggtaatcccc aggacgtagg ccttgtaaag tcagatctcc
    31981 gtatttttca cgttttagtt tttgcacagg aagaccaact gcttgaagca tatttttcac
    32041 ttgatgatta cgtccttcgt ggatcgtcaa ttccacgata ctgtggtttg tcttaggatc
    32101 cactgataaa atttggaatt tagctggaga tgtttttcgt ccttcgatct ttattccttt
    32161 tgtcaaaggt gcaagcatcg attttgtagc gatccctttt acctttgcaa cgtaaacttt
    32221 atccacttca tgttttggat gtgtcaggcg ctgagcaaaa tctccatcat ttgtcagtag
    32281 caagatcccc gaagtatcgt aatccagtcg acctacagga tagatacgtt cttttaccat
    32341 cggcaaaaga tccgtaacaa cttttcttcc tttgtcatct gcaacagcag aaataactcc
    32401 tttaggtttg tacaataaat agtaaccgta ttcttcttga taaattggga cttcatccac
    32461 ttccacagtg tcatgtttcc caacttttat ccctaattcc cttacaactt gtccgtttac
    32521 ttttacacgt cctgcaagga tgaattcttc tgcttttcga cgagaagtga tgccagcgtg
    32581 ggcaattact ttctgcaatc tctccattac aattattctc ctttattcat tccatcaaag
    32641 aatagatctg gtgacacgtc ttcttcaagt gaaatttcca tttcttgtgt atcaggcagt
    32701 tcttctaatg atttcaaacc aaaataatct aagaagtagc cagttgttcc atataatatc
    32761 ggccgacctg gtccttctac tctacctttt tcttcaatca gtttatgggc agctagacgc
    32821 tgtaccgctc ctgaaacctg aactcctcga atctgctcga tttcagctct tgtgattggc
    32881 tgtttataag cgatgattgc taaggtttcg acggcggctt gagataatgt gtttgatgtc
    32941 ccttgcgcat atcgcttaag taagggagcc agctcttttt tagtcgttaa tacataatgg
    33001 ctttccgttt caaaaatatg caaagctgag tgcgagtttt gagtgtaaga ttcttttaat
    33061 tctttcacta aacttgtgac ttctgcttcg ctgagattta acagtccgct taattcttca
    33121 ttactgatcc cttcctcgcc aacaataaaa agaagggctt caagttcact taatttcgtc
    33181 atgttcggta tctcctactg ctggataaag cataattgat tcatagtttt ctttttgttg
    33241 cgcaatgatc tgcccttttt tcatcaattc tagcaaagcc ataaaagtag tgacaacttc
    33301 acttttagaa ggtgactcaa aaaaagaatc gaaagtacag ccatgcgcat ttctttttat
    33361 acgctcttcg ataaatacca ttcgttcttc gatcgttgtg tcatcgggcg tgatcgtttt
    33421 ttccactttt tgagaaaatt tcttacgttc taagattttc tgcatggcac ggatcaaatc
    33481 gatttttttg acttgtcctc gcttcaacgg cttttcagca tccgctaacc catcgatgtt
    33541 cataggttct tttgtgtaat aaagacttcg ttcctctgct ttttctgtca gtctttcagc
    33601 tgcatattta tatttacggt attctaaaag ctgagtgacc aattcttctc gaggatcttc
    33661 ttcccataat tcttcatcgt ctgtgacgaa ttcttggaca ggaagcaatg tcttgctttt
    33721 gattgccatc aatgtagcag ccatcactaa gtattctcct gcaacagcta gctcgagtgt
    33781 tttcattgta tggatataat tcatatattg ctccgtgatc gtagcgatcg gtatatcata
    33841 tatatcgatt tccatttgct gaatcagatg aagcagcaga tcgagaggac cctcgaatac
    33901 atctaattta atattgatct ccgtcaactc attcactctt tcttcttttt ctttgctagc
    33961 cattttccaa tgtaaggctc tgtgatttca gttgcacgga tggagacatc cgggtacttt
    34021 tcctgtaatt catctaacat ttgaaagccc atattctcac cgcgataaga cggattcaaa
    34081 gaaatgtcat ggatgatcag ctgatcgttc gttgcccagt aaataccaat aatcccttga
    34141 atgttggttg aaccttctgg cgtaaagaaa aaaagttcat aatcgtcttc agattcgtac
    34201 gtatcaatct ccttcaacaa agaagtttta cctgtcagtt tcttatggaa actcaatagt
    34261 cccatagcaa ttttacggtt ttctttccgg taatgtgtta acatcttctc accttcctat
    34321 gactaagccc ttggaaaatg ctgcttataa acatccgtca ttcttttctt cgtgatatgt
    34381 gtataaatct gagtcgtaga gatatccgcg tgacctaaca attcctgtac tgtccgcaaa
    34441 tcagctccat tttctagcag atgtgtggca aagctatgcc gcagcgtatg tggtgttact
    34501 tctttgtaaa tcccagcctc tcgtactaac tgcttcagat ttttccagat tccttgccga
    34561 gaaagacctt tcccatgatt attgacaaat acatgcattt cagatgcgtc ctttacaagt
    34621 aatggacgtg cttcgtctaa gtagcgttcc agccattgga tcgcataatc acctaaagga
    34681 atgatccgct ctttatctcc ttttccaact gtctgtacca gtccaagaga aagatgaaga
    34741 tctcccaatt tcaatccaat caattcactg acgcgcatcc cagtagcata catgacttcc
    34801 aagattgcac gatctcttat ccctaacgtt tttgtcgtat caggtgcttc aatcagcttt
    34861 tctacttctg tcagagtcaa tgtacttggt aatttctgtg ctttcttcgg cgtatcgata
    34921 tgttgcatcg gatcatgatc agtgatacgc tcttgccgta aaaactgatg gaatcttctg
    34981 agacttgaga tcatccgagt aacagttgct gtcgcttttt tttcttcatg caacttctcc
    35041 agaaaacgga tgactaaaaa gcgatcgaca tcctgccatg aagcaatttt ctgctcctgt
    35101 agaaaatgca agtactgttc caaatcacgt tcatagcttt gtcgtgtatt ggcggacaat
    35161 ccccgttcaa tattcaagta gtggagataa tcgttgattt gctctttcat aataatcccc
    35221 ctcatgcctc tctatcatat cagataccgc ttcattctga tagtcttttt ccataatctg
    35281 agcaaagaaa aagccgtcta gacactcaga cggactggtt tcttttttct ttttcatagc
    35341 attcctgaca gattccatgg aacgttagcc gatgatcttt tactaaaaaa tgataacgtt
    35401 cttctactac ttgctccact tcaagtaaaa gatcctcttc tacttcctcg atcgtgccgc
    35461 agttcaagca tagtaaatga tgatggaaat gtttggcgcc ttcttttctc aaatcataac
    35521 gggctaatcc atcattaaaa cttactttat cgacaatttt caattctgtc aagatttcaa
    35581 gggttcgata aacagtagca agtccaattt ccggactctt ttttttcact aaaaagaaga
    35641 tttcttctgc agataaatgc tctttctcat tttccaaaag cacaagcaga gtagcttctc
    35701 tttgcggtgt cagcttaaac cccgcttcat gtagctgctg ttttgttttt ttcatcgccg
    35761 aaatagcatt catgaccatt tactcaaccc caacaattta taataattac aaataaaaaa
    35821 agatataaca ctatgttaat taaaattatt ataagctagt taagaaaaac atgcaaggga
    35881 ttctcaatta gattttttga ctagttttgt cacaccgttt tcttgtgtaa tcaatccttg
    35941 tttcaaaaga ttacctaatg cgcgcttaaa tgctcctttg ctgataccaa aagctttcat
    36001 gatttcttct ggatccgatt tatctgtaaa gttgatttgc tgatctgcag aacgttctaa
    36061 aatggttaaa atcatttgtg catcatctga tatagcttca tatgctcttg gcttcaatga
    36121 aagattcagt acaccgtctg gtcggacacc aatcactcga ccttttatct gctcgcctag
    36181 acgaggttca ttatatcgtt cagaaggatg gataaaccca agatagaaat cttccgttaa
    36241 aacgtacgta cctgttaatt tcaagcggta tgctgttcct atcacatctt tgtttttcat
    36301 ttcttctgtt ccatatttgc ttaaagattt gaatattttt tcatctgcta aactgcccca
    36361 aatacggtct tttgcatcaa ctctaaggga aatcatcaag cgatcccctt ttttaggcca
    36421 tagctctgtc attgtcggca attcatcaag agatacgaca aagtctttat ctggaagacc
    36481 gatatcgaca aacgcaccta aatcttttcg tgtagcagta acgattccaa aagcgtaatg
    36541 accgatacgg cttttaggaa tttgtgtagt gatccgattt tcatgttttt gattttggta
    36601 accgaatcct tcaactgctt caccgatttt gtgttcacct tctgatttgc ttaagccaaa
    36661 ggtttgtccg tttttttgga caaagtagtt tttttcattt tcatcaataa taatacctgt
    36721 aaatatagtt gctaataaat cgttcatttt tttctccctc tatagtgaaa ggttttctta
    36781 atacatttct ttgctatcat aacataatgt tatctacttg aatagctgag ccttggtttg
    36841 gaaaggtgaa aaaaggagta tgacaacagt tgtgtcatac tccccttttt cgaataaacg
    36901 gtgttaaacg gcaggctcac agcctctttc atttgcctaa aattaattgc tcccatttcc
    36961 tccgaaccat ccagttttac agttgatggt tacgatgtaa aatggcaaag cactttctcc
    37021 aatgcctttc atcaatgcag attttgttga agtggcatat agattataat agccttcccc
    37081 attttcgatt ctggcttttt ctaatctgta acctccttca acgatatatc cacgttggat
    37141 acatgtagca atcactttgc tttggacacc tggtgcccac gtccatgctg gattagatgc
    37201 atcggcaata tcacttgctc tggcagattt gattggctga ttcgctgagt ttgaagagga
    37261 aggggtcgag gatgccgctt cttgctgtgc tgcttgtgcg accgcagctt gttgagcggc
    37321 ttgagctgca gctgctgctt gagcggcttg ttttgcttgt tcttcagctg cttttttcgc
    37381 ttcttctgct tttttctttt cctcttcttc ttttttctct ttatcagtga gcatcttatc
    37441 tacttgtttc aattgttcat tcaactgagc agatagatct gtatttttca ctttttctac
    37501 tgcttctttt gccgtcttgt attggtcgcg tgttgctgaa tccactactt ttccgtcttt
    37561 aaagactata gctgtttttt ctttagctgt ttgcaattca gcatattgat ttttagcttc
    37621 tgtttgtgct tgttgaatca attttccaaa atctgtgtta tccgtttcga ttgcttggat
    37681 ctctttatct gctttcaact tgatgtcatc tgctagatga tcatcgacga tggcaggtgc
    37741 tatgaaccac tcattcactt tttggatatc tcggatctta gattggatat cttggtaagt
    37801 tgtttctaac tctgaatatc ctttttcatt cttaagactg gataaatcat tttttagttt
    37861 tgataaatct tgatgaatca tatctgcacg aataaattga tggtgatcat ctgtataaaa
    37921 ggcagcaagt ttctcatgaa gttcttccat ttgttgctgt ctttttgctt ctgctgctac
    37981 ttcggcttct actttttgat gatggtcata ataagcccag cttcctccac cgagtagaag
    38041 tactgctgct aaaacgagat aaggtacttt ttttgatttt ttcttttgcg ctttatctgg
    38101 atctgttttt tctaagtttg attcttgatg tgttttttca gtatctggca cgattgttat
    38161 tgatttcgct tctgcttcaa acagcttagg ctggttctct tctttttcta tttttgcttc
    38221 aagatcagta ccctctttgc ttacagattg attttcagca tcggattcac tatttatttc
    38281 atcgggctct gttgactttt tgtctgtgtt ttcgagtatt tcttttttct ctgcttccgg
    38341 cagtttattc gttttttcct tttctaattg tttttcagta aatacttcag ttttttctgt
    38401 gatatccgtt tttacgtctt ttatttttac tgtttcttta cctgtggcgt tttctctatc
    38461 agagtctttt tcagtttctt gttccttttc tggctctact ttgttttcct gtgcaatttt
    38521 ttcactgatc attttgagca gctctttcgc gtgctcttct tttgtatctt cacgatgtgc
    38581 tttgatataa gcggatagta tcgaattttc ttcctcatta tcgacgattt ctttcagttc
    38641 agctactctt cttttttgtt ttgattcaag cgtttcttct tgattctttc ctgattcttc
    38701 cgaagtctca tgaacagttg ctttcttctg ttcatttttc tctatattag ttacttgatc
    38761 gtgagcaggt ggttcttcgt cttctggttc tgctttttcg aataattcca ttacagattc
    38821 tagaggtaaa tctttcaatt ccgaccattc gattggatcg tttaattctg tatcaggaaa
    38881 gactgcatct tcagttgatt tatcctgcat agctgcatct gtatttgttt cttcaattga
    38941 ttcttttatt tgctgatctt ctgcagttat ttgatgaccg cagtctggac agatagtatt
    39001 ctgctccatt tttttcgatc cgcaatttgg acattttttc atcacttagc gatcggctcc
    39061 tttactaggt ttacgctacc atttaatcta tcatattcta tacgtcacgc caatagctta
    39121 tttttaggat ttacttaaaa ataatgaatg aatttccttc ctatagaaaa aaaagcttga
    39181 gactgcgtag tctcaagctt tttttctttt aaaataaacc aaacagtttt actgatttct
    39241 cttctggtca atagtccacg cttattctaa gaaatctttt aattgttttg aacgagaagg
    39301 atggcgcaat tttcttaatg ctttagcctc gatctgacga atacgttccc gagtaacacc
    39361 aaatactttc cctacttctt ccaatgttct cgtccggcca tcatctaaac caaaacgtaa
    39421 gcgaaggaca ttctcttccc gatcagtcaa tgtatctagt acatcttcca attgttcttt
    39481 tagtaactca taagctgcgt gttctgctgg acttgttgct tcctggtctt ctatgaaatc
    39541 acctaagtga gagtcatctt cttcaccgat tggcgtttca agggaaacag gttcttgcgc
    39601 gattttcaag atttctcgga ctttttctgt tggaagatcc atttccgcac cgatttcttc
    39661 tggcgtaggt tctcttccaa ggtcttgcag caattgacgt tggatacgta tcaatttatt
    39721 aattgtttca accatatgaa ctggaatacg gatcgtacgt gcttggtcag cgattgctcg
    39781 agtgatggct tgacgaatcc accatgtagc ataggttgaa aacttaaatc ctttgcggta
    39841 gtcaaatttt tcaactgcct tcatcagtcc catgtttcct tcttggatca agtcaaggaa
    39901 ctgcatgcct cgtcctacat aacgtttagc gatacttact actaaacgaa ggtttgcttc
    39961 tgccagacgc tgtttcgctt cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc
    40021 ttcttccgca gttaaaaggg agacacggcc aatttctttc agatacatac gaaccgggtc
    40081 attgattttt acaccggttg gcgcagaaag atcttctgtt tttgcttgtt cagctttttt
    40141 ctcagcactc ttcaagctgt gaattgatgg atcaccattt tcatccacca cactgatacc
    40201 tgcgtcttca atcttttgga tcaatttgtc catttcatct gcattcaaag tgaatggtgt
    40261 agcaagctga ttggataaat catcatatac aacttgtcgt ttgggtttgt tttctttgat
    40321 gaaagctgct actgctgctt catacgtttt tttatctgtt ccatttgcca taaaagaagg
    40381 ccccccttga gtagctattt ggcttgtttt aactgtttcg ttaaattgat gatttcaatc
    40441 gtcaattcaa gctcaagctg tttgtttcca gttcgtctgg cttcttgctg catttgtttt
    40501 tttaatgtta tttcatcagc taagccagat ttgctgatca catctagcaa gtcttggatt
    40561 tctcgtggcg aactttcttc tgccaccggt aaatattcga tttccaccac caaccgcctt
    40621 aaattttcat cttgcaaaaa gtctaaaaac tctgcaaggt tgaattcata atgttgtgag
    40681 acatatgcat cgaataaaaa ataaacttct gcatatgcat catgaataaa ctgaaattct
    40741 cgttctttta aaatattacg gacagaaact tctttaaatg cacgatataa tagaatctgc
    40801 tctgcctttt ctacttgcgt caaaggtctc tttttccgtg cagtttcttg tctcgtaacc
    40861 tgagtgtgtt gactttcctg aggttcttga cgattttgct gatttgtcct tcgaagatcg
    40921 cgcagttgtt tttgtatcgt ctctctgccg atctgaaact ctgttgacag ttgagataga
    40981 tacatatctt gttcgattgg tgaaacaact cggctaagtt caactagtaa ttcttcgagg
    41041 tattcgatct tgtctttttc attatctaaa tttttccctt gttttaaata acgtgattta
    41101 aaagtaaaga ctgtttcgcg cccatgagcc actaattctt ggaaagatgc ttctccatac
    41161 ttacggagat attcatcagg atcaagcttt tctggaacga ctacgacaga aagtgctaat
    41221 cgactgtttt gccgaagcaa ttcaatccct cgattggttg cttcaaaacc tgcgttgtct
    41281 ccgtcatagc aaaagaccaa ttctttcgtc agcttttcca tttgttggat ctgttgagct
    41341 gttagacttg tccccataga agctaatcca gttttgatac cagcctgcca tgcagcaatc
    41401 acatccataa acccttcgaa caacaaaatt tctccgtttt tccggatatc gcttcttgct
    41461 ttatcaagat taaataaaac ttgtctttta ttgaacagtt ctgtttctgg actattcaag
    41521 tatttgggct gatctttttc ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg
    41581 atcgtctggc cttgagaatt acgaagtgga aacatcactc tttgataaaa acgatcagac
    41641 atacggccat catcatgcgt gacaaacaat ccagagcgtt ctaacaattc atttgaaatt
    41701 gattcattct tgaatacttg ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg
    41761 aattcttcaa ttaattctaa agacaaccca cgactttgca aatattctaa agctggctgt
    41821 ccagcttttg tatgcaatag catgtgatga tagatttccg ctgctttttc atgagcagca
    41881 atcagtttcc ccgtagcaga ttcaggattt tggacagcta tagtacgctg acgccattga
    41941 tcttctaacg gtatctgttc gaagtctgcc acttttacca ctgcttctgg aaaacttaga
    42001 ccttctagtt cttgaagaaa agtaaacacg tttccgcctt taccacaacc aaagcaatga
    42061 aaaatctgct tgtcttctgc aacagaaaaa gacggtgtac gttcttcatg aaaaggacac
    42121 agccccaagt aatttttacc agactttttc aactggacat attgtccaat cacctcaacg
    42181 atattcgttt gactgcgtac tgtttcgata acctcttgag gaatccgttg agccattttt
    42241 tcaccccctg ctctattgat atcttttggg tttaacagaa gatatttcag gagaataacc
    42301 ggtaaaaaat cgcactaact gaaatcagtg cgattttaaa ggacacaatt atacatgttt
    42361 gattgtatca tatttcattg ctttttcaag tataacacat ttctatctca ttaagcatca
    42421 atttgctact cttttcagta actgattttg aatatcagcc ttccagtaca atactcgaag
    42481 aacaatattt acgagcatga atacaaagaa gtatcctacg ctctctccca accctaagtt
    42541 tacttccaaa attccatgga acagcattgc agctacatat atccctacga tcgtcgttac
    42601 tgctaggaag atgcgcaatg ggttgaaggg ataacaagct tttacaactg ccatgcaact
    42661 gatcccaatc agcaagtaat acatcaaagt agtagtttca gcatttcccc atgcgaatgt
    42721 ttttccaagt agatacaccg caatgatatt cactactaca agcaaggcat ttggtaaagc
    42781 attaataagt gctgtaggca aataacggta agtgattttt cttttatctt gttcaaaaga
    42841 taaaaagaag gaaggatacc cttcaatcgc taaatcaatc agcgtgattt ggatagggat
    42901 aaacggaaat ccaattgctg taacggcaca tataatcgat aaaataaatg aataaatcgt
    42961 tttgataaaa aagatgcctg agactttcgt cacattgttg accactcggc gtccttcaaa
    43021 caggacttct ggcaatgtag taaaatcaga gtccaacaat accagattcg caatctgacg
    43081 tgttgctcca tcaccttctg ccatagcaat gctgcaatct gcttctctta acgccaaaac
    43141 atcattcaca ccgtcgcctg tcattgcaac cgttctgccg ctttctttca attcatttac
    43201 cagcaatttc ttttgctgcg gactcactcg tccaaaaacc gtatagcgat gagcagcttc
    43261 tcgcacttcc atttcttcag taatttgaga aagatcgaca tacgcatcat aattcggtaa
    43321 tcctgcacgt ctagcgatat tggagacagt aaaaggattg tctccagaaa tcactttcag
    43381 atccacacct tcctcatgaa gataagctag tgtttcttga gcgttttttc ggattggatc
    43441 atcgatttca aacagagcaa ccggctcaac aaatgctggt ttttcttcgt tcaattgctg
    43501 atttggagcg atccccaaca taagtacgcg atagccattc gcttgggctg tttttagact
    43561 gtccggaagc cgatcttctt ccaccaaacg ttcaggcgct cctacgtaca cgactcccag
    43621 ttctggaaat tccatcgcgc cccatttgcg atcagaagaa aatgcgactt tgtttgtcac
    43681 atcataccga ttagaaagct caaaataatc tctcatagcc cgcatagtga tgttgttatc
    43741 actgctttct gccagatacg tccccataat ttctgggatg gctttctcgt acagcggatg
    43801 tagtgcttct acccgctgga tactcatttt cccttcagta atggtccctg ttttatccaa
    43861 gcataatgta tcgacatggg ccagcgtttc aattgaatac atgtcttgaa caaggattcg
    43921 tttttttgcc agtttggtta cccctgtcgt taatgcaata cttattagca ataccaaacc
    43981 ctttggaagc atgcctaaca aagctgctgc agatgcaata acagaaattt tgatagaagc
    44041 atcgcggatg aacagtgctt ccaaaaatag tatgatcccc aaaggaataa tcacgaaact
    44101 agtgaatttg gatactttgc gaatcgaatc tacaagttca gaacgaatag ctttgtgagt
    44161 ctttgcctcg ctagtcaagc ggacagcata attatctgca cctactcgga taacttcagc
    44221 catgacagaa ccgcttgtca aaaagcttcc agaaagcaat tcagctccag tttctttgac
    44281 aatcaaatca gattcgcctg tcaaaagagc ttcatttgct tctgcccgtc cagatataac
    44341 ctgtaaatcg gctggtatct gttctcctgc tccgattttt accaaatctc ctaataccag
    44401 ctctttagta gaaatcgatt gttctttacc gtcccggatc actagtactt ctgattggga
    44461 aacaatcgat aatttccgta caagatttct tgcatgtatt tcttgataaa ttccaataac
    44521 gatattgacg acaataattg ctaaatacgc catgttggaa tacgcaccta caaataacag
    44581 acaaatccca ataccaaagt tcagaaaatt gaataacgtc aatatattat cgctgaatat
    44641 ttgttttgta ctttttgatg catcttcctg gtagtcattt tgctggccag aagccatttt
    44701 ctgaactacc tcttgctgtg taagtccatt cattttcttt ttctccattt tccgccaact
    44761 ttcaaaaata atcacgtgtt taagtctagc gtgttccaag attaaaaaca acttgttttt
    44821 ataaaattta tagaaaaaca cacgaatgat tttacaaaaa agatgccaac ctttttgttt
    44881 gaatcacccg tgtgctgttt ttattatcaa tctatcttta cttatattgt tcctccaaac
    44941 ggctcatcca ccaacctagt gctgtaccgg ctataaaaag aacaggacaa gctagtaccc
    45001 cagcaactcc caatcccgcg taatcggtag gaatgatcat aatcgttgaa gcaaaaacta
    45061 cacctaaaat gaaatggaat agcttgctat atgcctttga gaaaatgtaa tccatgattt
    45121 ttgacaatcc aagaacacaa agaatcccac caattccgat cggcacaatc acttcaagtc
    45181 gtacttcttt aaatccgtca gccatcgctt tatacaatcc catatacacc aaaaaattag
    45241 aagggctcaa tcctgggacg atcataccca aaccaatcaa tccgccggca atcagccatg
    45301 ttccaaaatt ctgatcgact tgcccgtcaa ataattgagc gccgtataat aaaaacaaat
    45361 aagccagaac aaaagtaaca accatgatga tcacatcaga agtatctctt ccttttttcc
    45421 ctgcttcttt ccataacgca ggaacagttc cagcgataca tccgacaaaa aaccataata
    45481 tcgttgtttc atacgcacct aataaaaaac tgactaaaaa agaaagtaga aagatgcctg
    45541 tcactccgcc tagaccaaca ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa
    45601 ggtgggctaa aaaagaaatc agccgctcat acatgccaaa gacagctgct aatgcaccac
    45661 cgctgactcc tggcaagata aatcctgaac cgataaacat tcccttcaca aaccgtaaca
    45721 tccagtcttt ggttgtgtac cccacttctt gattttccat tttttgcctt ttttccacta
    45781 tttgcgtact ccttctttca tttaatttta ttcatgattg aacatttcct gctattttac
    45841 ttaaagactt tttcataggt ctctattatt ttttcattca ttttgacaat atcaggataa
    45901 aacatgttac ctccattatg atagagatag ccgccattgt aaagcaagga ctggatccgc
    45961 caatatcggt attgtttccc ttcctcattt cctaaaagtg ggctcagcac atctttagaa
    46021 tacgcttctg ctaattctac tgtgttttct ccgccatgat tttttacata ggcaatataa
    46081 tctttcccaa aattatatgc ttggacagct gtccataaat cgcatccttg tttctttgct
    46141 tctactatca tttccgccaa atatttgaca ccttgatcaa tactgtcagc tggattatca
    46201 atgatctcag cttctccatg cagactttcc gagctctgca tgagatctgt cccttttcct
    46261 tttgattcgg tcaatattat cgccttgatc aaattctcgt agttttccat atgataattt
    46321 tttgcagctg ttgttatttc tatttgatag ctttccactc gttttaccga acgataagtt
    46381 cttcctcata aaaacaacac cgcaccaata accgtcagaa tgatcatgta aaaaagaata
    46441 cggataatag ataatcgttt tcgcttcatt tcatcaccta gttattttga catctttttt
    46501 tatcttacta tttctttatc ttcttggcta tttcttcctt gatttctgtg tacgccttaa
    46561 gaaaaaatga attttcctgt attttttgac atcttacgaa aatgatcgtt gtttttcagt
    46621 aaaaacaatt gcccaatgac tgatataatc gttatacttt acacagtagg tgattatgtg
    46681 aaaaaagaaa agaaatatgc aaaaatgcca gatgggagcg aaatttacta tgaaaagagc
    46741 ggtcaaggct tccctctctt tttactgcat ggcaatgacg gcagcggccg ctttttttca
    46801 gaacaggtcc ctgtactcga acgctactat accgtgtatc tagtagatag ccgaggacat
    46861 ggtcgttcga caaacgaagc atctatgttg aattttcagt taatggccga ggatctgaat
    46921 acgatcatgt tgctggaaaa aatcgatcag gcagattttc ttggatttag tgatggagca
    46981 aatctagcac tggtatttgc cagcagcttc cctaaaaaag ttcatcgatt gattttaaat
    47041 tctggaaaca cgcttgttaa aggcgttcgt ttttctgcca gagtcatcag taatattcat
    47101 tatgcatggg tatggttact ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt
    47161 atcaaacttt tgctgcatga catcgggctt acagaaaatg atttaaaaaa aatcaactcc
    47221 cccactttga tcatcgtggg aaaaaaagat gtaatcaagc taaaacattc tttgtatata
    47281 gctaaaacta taccaaaagc ttcctttgtg cttgtgaaag aacaaggaca tgaattagct
    47341 agaaaagatc ctgaacgctt caatcgagaa gttcttcaat ttctttctga aacataacta
    47401 ttaggaatag aaggtgattt atttgttaaa aaataccata caatggctga aaaaccacct
    47461 aggactgttc aaaacaatct ttttgatttc tgtcattgtc attattgtcg gtgaattgat
    47521 gtccatcggt aaaactctat ctatccagca attaggagaa acgtttgcca ctatacctgt
    47581 atggaaaagc ggactaatgc tattgattgg tcttatctct gtgttaccta tggttggtta
    47641 tgatattatt ttgaatcgga tgcttgaaca aaagcagaat cctcgttacc tgtttgagac
    47701 aagctggctg atcaatacga tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg
    47761 gcttcgctct gaactatatg gacaaaagaa agagagcaga aacgtgatcc aagcattatc
    47821 gaaagttttc ttgtttttga tggctggact ttctatttac agtctgcttt cttttttact
    47881 tgtagtcttc acgccagttg ctcctttttt gaagcaatat tggatatggt tgatcggcgg
    47941 cggtctatat tttcctgtcg tctttttatt tactactttc aaaaagaaag gattccttgg
    48001 tggtacctca cttaaaactc aaggacagct attgctagtt tcattgttgg aatggaccgg
    48061 tgtcctgttt agtttccttt ctgttggtta tttaatggaa atccggattg atctgtggca
    48121 aacaattcca ttattcattg ctgcttcagt gataggaatc gtgtctatga ttccaggaga
    48181 aattgggagt ttcgatgtaa tgatgattat cgggttgtcc gctattggtg tgccaagaga
    48241 aatagtcgtt gtgtggatct tgctctatcg cctgttctac tacatcattc cgtttttgat
    48301 tggaatcgtc tttttcttca aaaatatcgg ttccactttt gatcagcgtt attctggtat
    48361 ccctaaacaa ttagcaacag aaattgcgca taaaatcgtg gttgtcttat tatacttttc
    48421 tggaatcatg ctagtacttt ctgccactat cccgcaagct tttacagagt ttcgctggct
    48481 tcatagtttg aacccattga aatttcattt tattatccaa tttcctagta tactattggg
    48541 atttctttta atcattatgg gcagagggat cgcagcacgt gtgaaacgag catatttgcc
    48601 tacaatcttc ttgattgttc tagctttact ttatgtttta ttaagtgatt ttagtttcac
    48661 accggtggtt ttcttgtcta ttctgcttct gatcattctt gcttctaaaa acgaattatt
    48721 tcgggaacaa ttgatctatt catgggagtg gcgtacgatc gatggaatca ttattggtgc
    48781 actgtccttg ctttacatcg ttattggggt atacaatctg ccggatttcc ctcatcggcg
    48841 tcaccatttt atttcctttt tcttatttcc ttctgaaaag atttggtttt cgggattgtt
    48901 ggccatcatc gctgttagtt tcatgatcgt tctatttgtt catttccttc aaggagaaaa
    48961 gaaacagatt ggcgaagcct ttaatgaaga aaaggccctg aagatcctta caacttatgg
    49021 aggaaactcc gatagtcaat tgatcttctt aaaagataag cggatgtttg cctatgaaaa
    49081 agacggtgaa ccaactgttc ttctccaatt cgcttgcttc aataataaat gtattgtaat
    49141 gggtgatcct tctggtaaga aagaagattt tccagaagca atcgaagctt ttatcgaaga
    49201 aacagatcga ctatgctatt taccagtttt ctacgaaaca agcgaagaga ttgttatgat
    49261 tttgcatgaa ttcggctacg atttcattaa gatgggggaa gaagcttatg tagacctgaa
    49321 tagttttaca acttcaggga aaaaaatgaa gggcactcgt gctgtcttga atcggatcga
    49381 acgagaagga tttacatttg atgtcttaca accaccattt tctactgagc aaatgagtac
    49441 tttcaaaaat atctctgata attggttggg ttcacgaaag gaaaaagggt tttctttagg
    49501 ctttttctct gaagattatc tacaaagagc gcctattgca gttgtcaaaa atacccaaga
    49561 agaaattgtc gcttttgcaa cgatcatgcc tacttacacg aacaataaag taggaacgat
    49621 tgatctgatg cgatatgatt ctaaaaaagc accttctggc agtatggatt tcttgttctt
    49681 gcatttgttt gcctatatga aagaagaaca tatccaatgg ttcaatttag ggatggcccc
    49741 tctttcaaat gtcggaactt ccagaaagag ttttttacag gaaaggattg catacttggt
    49801 ttacgagttt ggttctcatt tttattcttt ccatggatta agagaatata aaagcaagta
    49861 cgctacaaac tgggtctcac gttatactct atattctaga gatagttgga ttgcatatgt
    49921 tatgattgct ttgctgatta ttgataatgc gccagttgag cagacacaag gtagaataag
    49981 tggtttgaaa aaatggatac gaagaaagta ttaaaaactg cataccgata gatgaacact
    50041 ctcatgatga ataaaagaga ctgaagtaaa acgaaagttc atgcttctac aatctcttct
    50101 tatacaattg aagaacaaag agcggtccaa acagaaatcc tgtttggacc gctcttttat
    50161 tttaaacatt tttcattacc aaaatattcg agtcgcgttt cttatgcttc gatctctgta
    50221 acgatacctg aacctactgt ccgtccgcct tcacgaattg agaatgtcgt tccgttttca
    50281 acagcgattg gatggatcaa gtcaacatca atcgtcacgt tgtcaccagg catgaccatt
    50341 tctgtatctt ctggcaatgt gattgttcct gtaacatcag ttgtgcggaa gtagaactgt
    50401 ggacgatagt tgttgaagaa tggagtatga cgtccacctt cttcttttgt caacacgtac
    50461 acttctgctt tgaattttgt atgtggagta atggaacctg gtttagcaag tacttgtcca
    50521 cgttcgatat cgtcacgttg gatcccccgt aacaacacgc ctacgttatc cccagcttcc
    50581 ccgtaatcta acgttttacg gaacatttct acacctgtta caactgcttt ttgtgtttca
    50641 ggtttgatcc ctacgatctc tacctcatca ccgacacgaa cagcaccacg atcaatacga
    50701 cctgaggcaa cagttcctcg acctgtaatt gagaagacgt cttccactgg caatagtaat
    50761 ggtttatctg tatcacgttc tggtgttggg atatattcat ctactgtatc catcaattcc
    50821 ataatagcag cttcagcatc tggatcgcct tgcaatgctt ttaatgcgga acctttgatc
    50881 acaggagtgt catcgcctgg gaaaccatat tcgctcaata actcgcgaac ttccatttct
    50941 accaaatcga tcaattcttc atcatcgact aaatcaactt tgttcaagaa aacaatcagg
    51001 tattttacac caacttggcg tgacaataaa atgtgttcac gtgtttgagg catcggaccg
    51061 tcagttgcag aaacaactaa gattgcacca tccatttgtg ctgcccctgt aatcatgttt
    51121 ttcacatagt ccgcgtgtcc tggtgcatca atatgcgcgt agtggcgttt ttctgtttca
    51181 tattctacgt gtgcggtatt gatcgtaatc ccacgttcac gttcttctgg agctgcatca
    51241 atactagcat agtcttgagg atttgctagg ccttttttgc ctaatactgt agtgatcgca
    51301 gcagttaaag tagttttccc atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt
    51361 ttacttctgt cataatgttc ttttgccata atttacaaac ctcctaaata atatagatca
    51421 ctgaaaaatt tcagctctca cctttttatt atagtctttt tttgactaaa tcaaaaagaa
    51481 tacgtccgac ttactttttg taagattcga actaagctta ttatacgtca atattggtca
    51541 aagtcaacta agagcactcg tctgtcttat acgcgaaaaa agtacctttt ataaataaaa
    51601 aaatgtgttt tttgagaaaa acttcaagat agtccttaac tttcttttga agcaataaga
    51661 acaaaatgag gaaaagttta aaattcagct gttgccaaca actttcaata gacgaaatct
    51721 tctatttcgt taataataag gaaagaggtg aagtacatga aaaaattaat tggcgtactt
    51781 gtcgttttag gcatatttct cggcggaagt tttgcagctt atcattattt ttacggcggc
    51841 gaagcttatt atacaaaaat cacgacgaat ggcgaaatgt cagctaccca agcagataac
    51901 ggagagaaat tcacgactta tacctacaag caagcggctt ataataaaga tggtgaaaaa
    51961 aaacaggtga cgcttcgcga agaaagagaa catccattaa agatgaatgc gtatcttaaa
    52021 ctcaaagtca atcctcgtaa aggtgtatta agctgggaag aaataaaggc ttctgaagtg
    52081 cctaaaaatg cggcagaaaa aatcgataat tagtaaatgg tcttaaaatt ttattagata
    52141 aaaagaggct gggacagaag cgtttaactc caagaaataa gaaggagttg cttctgcttc
    52201 cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg tctcacgctc
    52261 tttttttgtt atattcgtct tttccgatcc ttctcttgac acaattcatt taactgatca
    52321 ctggtacttg aacacctaat attctagtca tttttataaa cgattcaata cttcatccac
    52381 atttttgatc atcacggata tcgtaccatc cggattattc acaaattcaa tcaaatctgg
    52441 gttgcgatat acatctactg gtacgatcag ttcgatcccg tttgatagct tgaatttttg
    52501 ttttccaaat tttttctcag aaatttcttg tacttctttt acaggaggtg cttctgggac
    52561 aaatccttgc tcttgtactt cttctttaaa agccattttt gccgtgatgt tttccttgaa
    52621 gactttttcc gcaatcatct catgatccaa ccgacctttt tcttctatcg tatcatagac
    52681 agcttctttc aaatcagcca tgatatcaaa ttcttctgtc tcaaattttt ttccaagatg
    52741 cttggctact ttttggatca cttttacatt ttcttctaaa gaaggtgcag gaacactttc
    52801 gatcactctt ccagaaaaat aaagctcttt tttgccagaa aactcatatt ttttttcaat
    52861 taattcgtag gtccaatcag tcaaattcac agcgaatgct tcatctgctt tttgtgtttt
    52921 cgcccctaga attgctcgat tcaaaatcaa gcggttgtcg atccctgctt cgtctgcttc
    52981 aacaaagtga gtgtaagcct cacgataatt gactttcaat aaagataagt acatcactgt
    53041 atctaattca tacaagacaa caaatacatc tgcactaggt gcttcttcac tttcttggta
    53101 gatatcgaac caccgctgaa cgattgcttc acttgcttcc acaaaatttt cttgagcgat
    53161 tttaagtgat tcaacgatca tgctttcttc tgctagttgt ccagtttttg tttgagcaga
    53221 agagattttt tggatcttct tttgaagaaa atcacgaata tattcttttg tcagatccag
    53281 ctctttttgt gaaaagactg gagaaccagt ttctctgtca ataatatgca gaattgcttt
    53341 ttttaaatag atatccattt tctcattccc ttctgtctta gtttacggaa aaaaaatcaa
    53401 aaataccaga atcaaaatca ttttctttta aacaaaaatt tcaagagtag aaacaaaatg
    53461 gattcttttt ttcgaaaaat gcgctacact tatagttgaa cgaataaaaa aggagctgaa
    53521 agtatgaatt cattaacaag cacttatcgt ctatcaaatg gttacgaaat cccggtagta
    53581 ggattcggca catggcaaac acctgatggg gatgtcgctg tttcctctgt taaagaagcg
    53641 ctggctgcag gttatcgcca tatcgatact gcccaaggat acaagaatga agaaagcgtc
    53701 ggccaagcga ttaaggaaag tggaattcct agagaagaga tattcttgac aacaaaatta
    53761 tggaatgcaa accacagcta tgaacttgtg atgtcttctt ttgaagaatc attgaagaag
    53821 ctccaaacag attatttaga tctgtttttg atccactggc caaaccctgt tgcttttcgt
    53881 gataattggg aacaagcaaa tgcagataca tggcgagcat ttgaagaact gtatgaagca
    53941 ggtaaaatca aagcaatcgg tgtgagcaat ttcttacccc accatctaga tacactagca
    54001 aaaacagcaa aaattatgcc gatggtcaat caagttttcc ttgcaccagg cgaattacag
    54061 acaactgtag ttgagtatgc aaaaaaacat gatatgatcc tcgaagcata tagtccatta
    54121 ggcactggta aaatcttcga tgtgcctgaa atgaaacaaa tcgctgaagc acatgacaag
    54181 acgattgcac aagttgcttt gcgttggtcg ctgcaacacg aattccttcc tcttcctaag
    54241 tcagtgacac caagcagaat caaagaaaat actgaattat ttgattttga attaacagaa
    54301 gaagaaatga aacaaatcga tcagttagat ggtgtagtcg gcaaagcaaa agacccagat
    54361 actacccaat tttaagtcga aaggtgattt tatggatcca atcgaacgct tgaattctct
    54421 ctcggaagaa gttacccaga cttttcattc cgattttgtc tttttgatcg atgcagagaa
    54481 aatccaacat tttcctgcaa gaaactggac acatgatcaa atcatcgaag aactgaaaaa
    54541 acggttcgat cattcgctaa tggtcaaacc ctggcacgaa catgaagtca tttattcacc
    54601 tgaactacct gtttttgcct tgataccgaa aaaatagaaa gaatggaacg gattatgtat
    54661 atatacataa tccgttccat tttttatttt tctttcaaaa aacgggaaaa tacgcaaatt
    54721 tttctgttgt aatttgcgta tacgcaagtt tttttgcatt actccttttc atttggctgt
    54781 ttttttctta taatcgaaag ggtatgagcg aaggagagaa catatgaaga aaaaacagaa
    54841 acaaaaatta ttaaatcaat tccgtccttc attggatggt gtaagaggac aattattccg
    54901 ttctctagaa gaagagagct tgcgacgtta tggtttgccg ttacaagtca tgcttgatcc
    54961 caaaaaccgt caaatcttaa cgatcggatt agctgggaaa acaaatgaag atacacggat
    55021 caacgtgcca atcgatgaca acttcaatac tgtattaaag agaattcgtt caggagaaaa
    55081 cgggattttc gaacgattcc gtgacaatct tcttattgaa atcgtgtctt actggaatga
    55141 tcaacgattg aaaaacgatg aaccaactgc gcaagctgtt gcgacaccag ttacagaaga
    55201 aacagtcagt gtgccagaag aaaaaacgga aaaaccaaaa gcggacacaa ctgaaaatac
    55261 acgctcaact gatacgaatg ccgctttaag ttttgctgat ttttcaaaag ccgttgctga
    55321 gtatcctaaa ttttacacag aacaaaatga acatgaagta gttattaatg aaaaaaatgc
    55381 tgatgaacct cgtaaattag caactatttc tatgactact gaaaatgagt tcgttatcga
    55441 aaaagcactc gaacggaaat acaaagtcaa attgactttg attcctttga tcgaacaatt
    55501 tgcagcgaca gcgattactg atcgataaaa attctatttg tcataaaagg ctgggattta
    55561 aaatcccagc ctttttagtc atataataat ttctacagca agtaaaaaga aattccttct
    55621 aacatcaggc tcaaatatca gcttgacctg tccaacaaat cacctatgat cctacaatgt
    55681 taggcgactt ctcttctacc tgaagatatc ttggagttgc ctgatttctt aagatgccta
    55741 accttaacta taaaaaattc taataataaa catattttct gatagagaaa aaagcggttc
    55801 tataatattt tgtgttggtg gaaaatcgaa agcgattttc tactaacact tttttcttcg
    55861 tttcaactca aaaagagact cacttttggt aaaatttagt cgaccaaaac gtcaccctac
    55921 caaaggagtc tctc
//
LOCUS       DAZKOR010000010        49387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000010 DAZKOR010000000
VERSION     DAZKOR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.010"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..2690
                     /gene="adhE"
                     /locus_tag="ACG8ZM_000520"
                     /gene_synonym="adhC"
     CDS             93..2690
                     /gene="adhE"
                     /locus_tag="ACG8ZM_000520"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0491509.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2919..4025
                     /locus_tag="ACG8ZM_000521"
     CDS             2919..4025
                     /locus_tag="ACG8ZM_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0491510.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4042..5070
                     /locus_tag="ACG8ZM_000522"
     CDS             4042..5070
                     /locus_tag="ACG8ZM_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0491511.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5076..5555
                     /locus_tag="ACG8ZM_000523"
     CDS             5076..5555
                     /locus_tag="ACG8ZM_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0491512.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5571..6866
                     /locus_tag="ACG8ZM_000524"
     CDS             5571..6866
                     /locus_tag="ACG8ZM_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0491513.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6877..7701
                     /locus_tag="ACG8ZM_000525"
     CDS             6877..7701
                     /locus_tag="ACG8ZM_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0491514.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7866..8765
                     /locus_tag="ACG8ZM_000526"
     CDS             7866..8765
                     /locus_tag="ACG8ZM_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0491515.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8891..9760)
                     /locus_tag="ACG8ZM_000527"
     CDS             complement(8891..9760)
                     /locus_tag="ACG8ZM_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0491516.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9913..11373
                     /gene="rhaB"
                     /locus_tag="ACG8ZM_000528"
     CDS             9913..11373
                     /gene="rhaB"
                     /locus_tag="ACG8ZM_000528"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0491517.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11370..12626
                     /gene="rhaA"
                     /locus_tag="ACG8ZM_000529"
     CDS             11370..12626
                     /gene="rhaA"
                     /locus_tag="ACG8ZM_000529"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0491518.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12640..13485
                     /gene="rhaD"
                     /locus_tag="ACG8ZM_000530"
     CDS             12640..13485
                     /gene="rhaD"
                     /locus_tag="ACG8ZM_000530"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0491519.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13487..13798
                     /gene="rhaM"
                     /locus_tag="ACG8ZM_000531"
     CDS             13487..13798
                     /gene="rhaM"
                     /locus_tag="ACG8ZM_000531"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0491520.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13880..15031
                     /gene="fucO"
                     /locus_tag="ACG8ZM_000532"
     CDS             13880..15031
                     /gene="fucO"
                     /locus_tag="ACG8ZM_000532"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0491521.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15158..15916
                     /locus_tag="ACG8ZM_000533"
     CDS             15158..15916
                     /locus_tag="ACG8ZM_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0491522.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            16016..16660
                     /locus_tag="ACG8ZM_000534"
     CDS             16016..16660
                     /locus_tag="ACG8ZM_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0491523.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16667..17674
                     /locus_tag="ACG8ZM_000535"
     CDS             16667..17674
                     /locus_tag="ACG8ZM_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0491524.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17690..18532
                     /gene="kduI"
                     /locus_tag="ACG8ZM_000536"
     CDS             17690..18532
                     /gene="kduI"
                     /locus_tag="ACG8ZM_000536"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0491525.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18552..19355
                     /locus_tag="ACG8ZM_000537"
     CDS             18552..19355
                     /locus_tag="ACG8ZM_000537"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0491526.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19604..20668)
                     /gene="fni"
                     /locus_tag="ACG8ZM_000538"
     CDS             complement(19604..20668)
                     /gene="fni"
                     /locus_tag="ACG8ZM_000538"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0491527.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20665..21750)
                     /locus_tag="ACG8ZM_000539"
     CDS             complement(20665..21750)
                     /locus_tag="ACG8ZM_000539"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0491528.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21763..22740)
                     /gene="mvaD"
                     /locus_tag="ACG8ZM_000540"
     CDS             complement(21763..22740)
                     /gene="mvaD"
                     /locus_tag="ACG8ZM_000540"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0491529.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22733..23677)
                     /gene="mvk"
                     /locus_tag="ACG8ZM_000541"
     CDS             complement(22733..23677)
                     /gene="mvk"
                     /locus_tag="ACG8ZM_000541"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0491530.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23993..24652
                     /locus_tag="ACG8ZM_000542"
     CDS             23993..24652
                     /locus_tag="ACG8ZM_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0491531.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24737..25642
                     /locus_tag="ACG8ZM_000543"
     CDS             24737..25642
                     /locus_tag="ACG8ZM_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0491532.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GRGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25644..26276)
                     /locus_tag="ACG8ZM_000544"
     CDS             complement(25644..26276)
                     /locus_tag="ACG8ZM_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0491533.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26427..26552
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26596..27120
                     /gene="infC"
                     /locus_tag="ACG8ZM_000545"
     CDS             26596..27120
                     /gene="infC"
                     /locus_tag="ACG8ZM_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0491534.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27192..27392
                     /gene="rpmI"
                     /locus_tag="ACG8ZM_000546"
     CDS             27192..27392
                     /gene="rpmI"
                     /locus_tag="ACG8ZM_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0491535.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27445..27804
                     /gene="rplT"
                     /locus_tag="ACG8ZM_000547"
     CDS             27445..27804
                     /gene="rplT"
                     /locus_tag="ACG8ZM_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0491536.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28056..29393
                     /locus_tag="ACG8ZM_000548"
     CDS             28056..29393
                     /locus_tag="ACG8ZM_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0491537.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29441..31570
                     /locus_tag="ACG8ZM_000549"
     CDS             29441..31570
                     /locus_tag="ACG8ZM_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0491538.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31545..32234
                     /locus_tag="ACG8ZM_000550"
     CDS             31545..32234
                     /locus_tag="ACG8ZM_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491539.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32642..33255)
                     /locus_tag="ACG8ZM_000551"
                     /pseudo
     CDS             complement(32642..33255)
                     /locus_tag="ACG8ZM_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(33391..33585)
                     /locus_tag="ACG8ZM_000552"
     CDS             complement(33391..33585)
                     /locus_tag="ACG8ZM_000552"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491540.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33635..34075)
                     /locus_tag="ACG8ZM_000553"
     CDS             complement(33635..34075)
                     /locus_tag="ACG8ZM_000553"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0491541.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34079..34924)
                     /locus_tag="ACG8ZM_000554"
     CDS             complement(34079..34924)
                     /locus_tag="ACG8ZM_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0491542.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35073..36491
                     /gene="pepV"
                     /locus_tag="ACG8ZM_000555"
     CDS             35073..36491
                     /gene="pepV"
                     /locus_tag="ACG8ZM_000555"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0491543.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36548..37495)
                     /locus_tag="ACG8ZM_000556"
     CDS             complement(36548..37495)
                     /locus_tag="ACG8ZM_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0491544.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37718..38068)
                     /locus_tag="ACG8ZM_000557"
     CDS             complement(37718..38068)
                     /locus_tag="ACG8ZM_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0491545.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38268..39347
                     /gene="pepA"
                     /locus_tag="ACG8ZM_000558"
     CDS             38268..39347
                     /gene="pepA"
                     /locus_tag="ACG8ZM_000558"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0491546.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39491..39811
                     /locus_tag="ACG8ZM_000559"
     CDS             39491..39811
                     /locus_tag="ACG8ZM_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0491547.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39833..40297
                     /locus_tag="ACG8ZM_000560"
     CDS             39833..40297
                     /locus_tag="ACG8ZM_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0491548.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40502..41107
                     /gene="ytpR"
                     /locus_tag="ACG8ZM_000561"
     CDS             40502..41107
                     /gene="ytpR"
                     /locus_tag="ACG8ZM_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0491549.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41146..42435)
                     /locus_tag="ACG8ZM_000562"
     CDS             complement(41146..42435)
                     /locus_tag="ACG8ZM_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0491550.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            42863..43342
                     /gene="rlmH"
                     /locus_tag="ACG8ZM_000563"
     CDS             42863..43342
                     /gene="rlmH"
                     /locus_tag="ACG8ZM_000563"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0491551.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            43427..43936
                     /locus_tag="ACG8ZM_000564"
     CDS             43427..43936
                     /locus_tag="ACG8ZM_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFQ0491552.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            44036..44686
                     /locus_tag="ACG8ZM_000565"
     CDS             44036..44686
                     /locus_tag="ACG8ZM_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491553.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            45137..46075
                     /locus_tag="ACG8ZM_000566"
     CDS             45137..46075
                     /locus_tag="ACG8ZM_000566"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0491554.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            46088..47140
                     /locus_tag="ACG8ZM_000567"
     CDS             46088..47140
                     /locus_tag="ACG8ZM_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0491555.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            47406..48041
                     /locus_tag="ACG8ZM_000568"
     CDS             47406..48041
                     /locus_tag="ACG8ZM_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0491556.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            48232..49023
                     /gene="msrA"
                     /locus_tag="ACG8ZM_000569"
     CDS             48232..49023
                     /gene="msrA"
                     /locus_tag="ACG8ZM_000569"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0491557.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT        15671 a         8809 c        10567 g        14340 t
ORIGIN      
        1 tcacaaaaaa gtgtttacat agaaaaataa atattgtatg atatacatgt gaattattta
       61 acaaataacc atgacccatt aggaggataa tgatggctaa agtaatagaa aaagaaaaaa
      121 cgaagacaat tgacgtacaa gcaatgattg atgatttggc agaaaaagca aatgcagcat
      181 tgaaagaaat ggaaagcttt gatcaagaaa aagtagacca tatcgtgcat gaaatggcaa
      241 tggcagcttt ggatcaacat atgccattag cgaagatggc tgtagaggaa acaggccgcg
      301 gtgtttacga agataaagca atcaaaaata tgtatgcgtc cgaatatatt tggcataata
      361 tcaaacatga taaaacagtt ggtgtgatca atgaagatgt ccaaaaaggg ttgattgaaa
      421 ttgctgaacc agtcggtgtg gtttgcggag taactccaac aacgaatccg acttccacaa
      481 cgattttcaa atcaatgatc gcgttgaaaa caagaaatcc aatcgttttt gctttccatc
      541 caagtgccca aaaatcttct gcagaagcag cacgagtggt acgtgatgct gctatcgctg
      601 ccggagctcc tgaaaattgt atccaatgga tcgaacatcc ttcgatcgaa gcgacttcta
      661 tgctaatgaa ccatccaggt atcgcgatcg tcttagcgac aggtggagct ggaatggtca
      721 agtctgctta ctctacagga aaaccagctt taggtgtagg tccaggtaac gtaccagctt
      781 atattgaaaa aacagcaaaa attaaacgtg cagtgaacga tttgatcgtt tcgaaaacat
      841 ttgataatgg aatgatttgt gcttctgaac aagctgtgat cgtggataaa gaaatctatg
      901 cagcagtaaa agcagaattc caagcacacc aagtttatat cgtaaaacca gatgaattgc
      961 aaaaattaga agatgcagtg atgaacgaag gaaaatatgc ggtcaatcca tcaatcgttg
     1021 gacattctgc gatggaaatc gcaaaattgg caggaatcag tgtaccaaaa ggaacgaaaa
     1081 tgctgattgc tgaattggaa ggcgtaggcc cagattatcc gctatcaaga gaaaaacttt
     1141 ctccagttct tgccatgatc aaagctaaca acacggacca tgcgtttgat ttgtgtgaag
     1201 gtatgttgaa tcttggagga ttaggacata cagcagttat ccattcagaa aacgaagagt
     1261 tgcatgtcaa attcggtctt cgaatgaaag cttgtcgtat tttagtgaat acaccatctg
     1321 cagaaggcgg catcggtgat atctataacg aaatgattcc gtcattgact ttgggatgcg
     1381 gttcttatgg taagaactct gtttctcgta acgtctctgc tgtaaactta atcaatgtca
     1441 aaactgtagc gaaacggaga aataacatgc aatggttcaa attaccgcca aaaatcttct
     1501 ttgaaaagaa ctctctattg tacttagaga aaatggaaaa cgttgaacgt gtcatgatcg
     1561 tttgtgaccc tggtatggtc caattcggtt actgcgatac agttcgtgaa gtattggcac
     1621 gacgtaaaaa tgatgtgaag atcgaagtct tctcagatgt tgaaccgaac ccatcgacaa
     1681 ataccgtata cgcaggaacg aaactgatgg cagacttcaa gccagataca gtaatcgctc
     1741 ttggaggcgg ttctgcaatg gacgcagcta agggaatgtg gatgttctat gaacacccag
     1801 acacatcttt ctttggtgca aaacaaaaat tcttggatat ccgtaaacgg acatataaga
     1861 tcgacaaacc agaaaaaaca caattcgtat gtatcccgac aacatcaggg actggttctg
     1921 aagtaacgcc atttgcggtc atcactgaca gtgaaacaca tgtgaaatat ccgttagcag
     1981 attatgcatt gacgccagat gtcgcaatcg tcgatcctca attcgtcatg tcagttccag
     2041 catctgtcac agcagatacc gggatggatg tgttaacaca tgcaatcgaa tcctatgttt
     2101 cagtcatggc ttcagattat acacgtggat tgagcctaca agcaatcaag ttagtctttg
     2161 actatttaga aaaatcagta aaaacacctg atatggaatc acgtgaaaaa atgcacaacg
     2221 cttctacaat ggctggtatg gcgtttgcta acgcattcct aggtatttgt cactcagtcg
     2281 cacataaaat cggcggggaa tacgggatcc ctcatggacg gacaaatgcg attcttttac
     2341 cgcatattat ccgctataat gcaaaagatc cacaaaaaca tgcgatgttc cctaaatatg
     2401 actatttccg tgcagataca gattacgcag atatcgcaaa attcttaggt ctgaaaggcg
     2461 aaacaacaga ggaattagtc gaagctttag caacggctgt ttatgaatta ggaaaatctg
     2521 tcggcatcaa catgagtcta aaagctcaag gagtcacaca agaaacacta gatacaaccg
     2581 tagaccgtat ggctgaatta gcttacgaag accaatgtac gacagctaat ccgaaagaac
     2641 cattgatctc tgaattgaaa cagatcatca ttgatgctta caacggataa gcaaaagaaa
     2701 gcgtagaaaa aagataagaa aatagatgaa aaagaacttg gaaaacgttg tttctccaag
     2761 ttcttttttt aatttccaag taactgaaag ttacttttgt tatttaacat ttaggtcaat
     2821 tttagacagt ttttgttcaa aaagcgatat ttatagtgga cggtattact gctatattaa
     2881 ggatataaaa tgaaagcgtt taaaaaggtg gcgtaaagat gattattaaa gaagagattt
     2941 ttgtacctaa agaattagtg aaagaaaaaa ttgacctgct cgttgaaaac ctgacaacga
     3001 tcaaagacga taatggtgaa tttttgttgg actttgatgg actgaaagta gatgacaaaa
     3061 gctggacagt gtggaattgg ccacaaggtg tcggtctata tggtatttat aaaaattacc
     3121 agatgacaaa aaatcctcgc gcttatcaag tggtcaatga atggtttgag gatcgtatgg
     3181 aagaaggcgc tccgcccaaa aacgtcaata caatggcacc gctattgact atggcttact
     3241 tgtatgaaga cacgaaagat tcacgctatt tgccttattt agaacaatgg gcagaatggg
     3301 tcatgaatga catgcctcgg acaaatgaag acgggttaca gcatgccaca tatggaccgg
     3361 aaaataagaa tcagttgtgg gacgatacat tgatgatgac agtgcttcct ttggcaaaaa
     3421 tcgggaaact gttgaatcgc ccagaatatt tagaagaagc tcgttaccag tttatgatcc
     3481 atatcaaata cttgatggat aaacgaacag gattgtggta tcacggctgg acgtttgaag
     3541 gaaatcataa ctatgccgaa gcattttggg ctcgtggaaa ctgctggttg acgattgcta
     3601 ttccagagat cattgaaatc ttagaattat cgaaagaaga tgcattgcgc aaattattga
     3661 tcgaaacact tgaagcacaa gtgcgtgcat taaaaactta tcagtctgaa agcggtttat
     3721 ggcatacgtt gttggatgat ccttcttctt acgtagaatc ttctgcaact gcaggatttg
     3781 cttacggtat tttgaaagcg gtccataaac gttatttacc acaagaatat aaagaagtcg
     3841 cttatcgtgc tatccaagga ttgcttgaac aaatcgatga aaaaggagaa gttcaaaacg
     3901 tttcaatcgg gacaggaatg ggggacagtt tagattttta tcggaatatc ggaatcacag
     3961 caatgcctta tggacagtcg ttaacggtgc tttgtttgac agaattgctc gtctcttatt
     4021 gttaaaagaa aggaaaccca tatgaaaaaa acatcactgc tgtttgcagc aagttcgctt
     4081 agcctaggtc tgctaggtct aagcggctgc ggaaataatt ctagtgtggc aactgtcgca
     4141 gatgcagatg taaatgagat agttaccttg cgctttgctt atgcaagcaa cagccagcca
     4201 gtaattgatg caatgaatga atttgggcgt ttagtaaaag aaaaaacaga tggttccgta
     4261 cagatccagt atttcccaga tggacaactg ggtggagaac gtgaactgat cgaattgaca
     4321 cagtcaggag cagtagattt cacaaaagta agtgcctcag cgttggaaag tttctctaaa
     4381 gattattcga ttttttcagt tccttatcta ttcaatgatg aagaacattt ctttaaggtg
     4441 atggataatg aagaaataat ggagccaatc taccaatcga ccaaaaattt aggatttacc
     4501 ggattgacct attatgattc tggtcagcgc agcttttata tggtagacgg tccgatccat
     4561 acgccggaag atttggaggg aaagaagatc cgtgtgatgc aaagcgaaac cgctatcaaa
     4621 atggtagagc ttttaggcgg ttcgcccgta cctatgggaa gtgatgaagt gtacacgtct
     4681 cttcaatcca atttgatcaa cggatcagaa aacaatgaat ttgtcttgta tacagctgga
     4741 catggtggtg tggctaaata ctattcctat gatgagcata cacgtgtgcc agatatcatc
     4801 atcatgaatg atgccatcaa agaacagcta actgatgagc aggaaaaagc cattgaagaa
     4861 gcagcgaaag aatcaacggt ttttgaaatt gaagcttttg caaaagcaat tgaagaagaa
     4921 aaagctttag cacaagaaga acatggagtc caattcaatg atgtagataa tacaccgttt
     4981 cgagaggcag ttgccccact tcatgatata ttcaaaaacg atcctaccta caaagacctt
     5041 tatcaaaaga tccaagagca aggagcgtag ctgtgatgaa aatgatttta aacaaggtat
     5101 tagagatcct gggagctgtt ttgcttgttg ggatggtcgc tgttgtttta tggcaagtat
     5161 tttccagaac ggttttagga aatccaaata cggtcacgga agagcttgtt cgattcggtc
     5221 ttgtctggtt tgccatgttg tcttccgcgt atgttgtggg acaaaaagca catttagccg
     5281 taacggtttt aagtgaaaaa ttaactggca aaaaaagtaa aattttagaa gttgttgttc
     5341 aaagtttatt tctggtattt gctgcgatga tcatggtgta tggcggatgg aatgctgtca
     5401 cattgacaat ggggcagatc tcgccttctt taagtattcc tatgggatat gtttatctgt
     5461 cagtacctgt atcgggggta ctcatcatta tctatagcct gatcaatcta atcgatacat
     5521 taagcaacaa gccagcaatt caagaaaaaa tataaaaatg gaggaataaa atggcaatag
     5581 aagccggatt agtgttattt tttgtatttg ttgtgttgct tgtattaggg atgccgattg
     5641 ctataagtat tgcattctca tcgatgatga ctttattgtt agttataccg tttgatgttt
     5701 ctgcttttag ttcagctcaa aaaatggtgg gaagtatcaa tagtttttcg ctgatcgcta
     5761 ttccgttttt cgttctctcg ggcattatca tgaataatgg agggatagct aagaaactag
     5821 tggattttgc gatgttgttc gtcgggcgcg tgccgggggc tttggctcat acgaatgtgt
     5881 tagggaatgc tttatttggt tccatctcta gctcggccat tgccgcttct accgcgattg
     5941 gcggagtgtt gatccctcag caagtggaag aaggatatga ccggaagttt gcgacagcgg
     6001 tcaatatcgc gtcagctccg acaggtatgg tcattcctcc aagtactgct tttatcatgt
     6061 attcacttgt tgctggtggt gcttcgatct ctgctttgtt tatgggaggt tatctcgtag
     6121 gcgctttgtg ggcgctagga atcatgctag tagcattcat cgttgctaaa aagaataaat
     6181 accctgttgt cgaaaaagga cagatggctc atgctggaaa aataataaaa gaggcgattc
     6241 ctagccttct attgattgtc atcattatcg gtggtatctt gacggggata tttacagcag
     6301 tagaagcttc agcgatagct gtcatttact ccctgttgat tgctatgttt tactataaaa
     6361 cggtaaaact aaaagatata cctaatatgt tgtttcaatc agtagcaatg tcagggacga
     6421 tcatgttctt attggctact tcttccatga tgtctttcgc catggcgttt accggtattc
     6481 ctcaggcaat tagttcgttg attatgggcg taacggataa taaatttatt attctgttat
     6541 tagtcaatct tgtgttatta ctggtgggta tgtttatgga tgtggcgcct gctatcttga
     6601 tttttacacc aatattttta ccaatcgtca caagtgtagg ggttgatcct gtacattacg
     6661 ggttgttttc aatcatgaat ctgtgtgtag gctccatcac gccgccagta ggtacaggac
     6721 tatatgtcgg tgcaagtgta ggaggcgtaa aagcagaggc aatgctgaaa ccgcttcttc
     6781 ctttttatgg ggttatctta gtcgtcttgt ttttgattac ttacttcccg caaatcgtca
     6841 tgtggctacc caatatgctg agttaggaga atagttatgt tgaacattac aattagagga
     6901 catgatcttt ccgaagtcca aacgatagaa gatcttgcag aaaaaacaaa agaacaaggg
     6961 attcatacac tgcagctcgc cttaggtctt tcgtttcctg aaacaccttc tggcgcaaat
     7021 gagatcaatt ctgggatggg aaattatgtc aaacgagttt tagaaaagca agaagtgacc
     7081 gtaggaattt taagttgcta tatcaatatg attcatccag atttaacgat acgacaagag
     7141 ttgctgacaa agttcgagca gtatcttcgc tatgcttctt cctttggtgc gtcgatggtt
     7201 gcttcggaaa ctggatgtgt tttgccggaa atccaatata cggaagaaaa tttcacagat
     7261 gaagcatttg ctgaagctgt gtccgttatc cgtcgcttgg taaaagctgg agaaaaatac
     7321 caaatgatgg tggggataga accaggtttg aatcatccgg tgtattcttt agcaagggta
     7381 gagcagctga tccaagcagt agattcggac tatctaggaa tcattttaga tccgaccaat
     7441 ttgatcacaa gtagtgatta tcaagaacaa gtccaattgg tagaagaagc ttttgaacgt
     7501 tttggtgaaa agatatgtgc ggttcatttg aaagattttc gtgtcgaaca ggaaaaaatc
     7561 gtgccggtaa atttgggaga cggtgtgatc gagtacacaa aaatcaaaga aatcatcatg
     7621 aagaatcgtc cgtacctgta tgtggttctt gaagaaacaa aagatgatgg gattcgttat
     7681 ggaagaagtc ttcttgaata atgtaaagaa gacgttggtt aactaatatt tagaatggag
     7741 ggggcggggc tagaagatgc cacgtctctt cttttttata agagtatata aaagcagctc
     7801 tttttgatta caaaaaacgt ggtataataa aattgtgcat aagtgaacaa aaggaggtgc
     7861 ggcacatgct ccaaaagaaa atcagcggtg aacaagtcaa tacgatggtt tctgatcagt
     7921 atgaaatcta tcatgtcaaa gatgtcgtca gccaaaataa aacgatctat catcatcatg
     7981 atttctatga gatccatgct acattaaagg gagaagcgac tttttatttg aacggacgac
     8041 aatttacgat caaagcagga agtgtgctgc tcatccattc caatgatttg catcgtatcg
     8101 tccgccaatc ttcagatgta ttcgagcgtg tctatatctt tgtcacccct tcttttctag
     8161 aaagtcgttc cacgccatgg tctaatctat cggcttgttt tcaaccaatg ggagagaaga
     8221 ggagtcgtgt attgcaaata gaacaggaag tattgaagca aaaattatca tttatcgacc
     8281 atcctcctga taaaaaaatt tacggtgaag acataaaata tgaacaagct ctggtagaat
     8341 acttgatttt tctcaatcaa gttgtacaaa aagaagagag tgcgcaagaa cagaatgctg
     8401 tagttcctaa tgagcgtgtg gaacaaatgt tacaatatat ctcaaaaaat ttatctcatc
     8461 cgctttcttt agaggaaatg gaaaagcagt tttttgtcag caaataccat atcacacgag
     8521 aatttaaaaa gtacactgga tttacgtttc atcagtatgt aatgaagaaa aagctgatct
     8581 atgccaagca cttgttgcga gaataccgca gctctagtgc tgtatatagt aaaagtggtt
     8641 tttcttctta tccgcatttt ctgaaagcat tcaagagaga gttcggcata acgcctaaag
     8701 aatttttgaa acgtgataca cagaaacagt tggttcatta tgaacatttt gaagatgaac
     8761 aatgagaatg atcactaaaa aataaccgca gtagctgctg ttgtttacat agaagcagaa
     8821 actgcggtta ttttattttt atctatatat ttcaaaatat tttgaaaaaa tgacgtacat
     8881 attatatgaa tcagctgaat ttacgaaagt ctctcggcga tagtcctgtt tttgctttga
     8941 acaggcggct aaagtaagca aaattctcaa tccctatttc ttcggaaatc attcgaaccg
     9001 taaattctgt ataccgaaga aggtctttag cgtaagaaag ttttttgctg atcaaataat
     9061 cgattggtgg agaaccgata aatttggaaa aatctttgct cagctgatat ttattcaata
     9121 agaattgttg ttctaattga tcaagtgtaa tcgtttcttt gaaatgttcg tctaagtatt
     9181 gcttcagttt gtaaacgtag gcgggaatat cctcttcttc gaaatctagc tgatactttt
     9241 gcaaaatcag ttcattcagc aactcgtgga tagctacaga ggactggaaa tcggtacggg
     9301 cattcgtgcc ttgttgcatg tggatcaatt ctgtgatcaa atggtggatc ttgttttctg
     9361 atggatcccc tgttgaatgg aaggtgttat ggccgtcttt tgtgaactcc tgataaaatg
     9421 attggacgtt ctgaccattc aaatggatcc aatccatttc ccatggttct tcgctgatcg
     9481 tttgataacg ctgatattct tggcagtcaa taaaaaataa gtctccagct tttagctgtt
     9541 gctgattatc attatatgta agcgaacctt tgccagacaa agtaaacttt atcaagtaag
     9601 aaggtaaatg tgctcgttca gtatagtaag ggattggtgc tttgaagtaa ccgatttctt
     9661 ggatataaaa aaataaattc ctgcttcttt gtgtaggcgt caagatcact ctttttgagt
     9721 cttccgacca tgagtggttg gctttttccc agtaatccat gtaaacactc ccaacttgtc
     9781 aagataatgc aattatatac caaaatagtg tgaagcaaaa tataagattc tgtattatga
     9841 tagcattgtt aataaatgat aacgctttat taataaagtg ctaatcaaca ataaagtaaa
     9901 agaggtggag aaatgaatcc aacatttgtt gccatcgata tcggtgcatc aagcggacga
     9961 ctaatgaaaa gtacgaggga tgcaaaaggt tctttgatct tggaagaagt acaccggttc
    10021 aaaaacggct ttcggaacaa agacggtcac gatcgctgga agatcgatta tcttatcaaa
    10081 gagattttga ttggatttga aaaattaaag cagacaggga ttgatcgcct ttttgcgggg
    10141 atcgatacat gggcagtcga ttactgtctg attgatgaac aaggggatcg gataaatgat
    10201 ccgattgctt atcgggacaa gcgtaccaat ggagcagtag aacagttttc tgaaatctat
    10261 tctctagata agttgtatga gcagacaggg attcagatcc agcctttcaa taccttgttc
    10321 caattgtttg ctgaagaaaa agaaaatttg gcaaaagcac ataaattgtt actgatacct
    10381 gattatttag ggtatgtctt tactggtaac atggtgacgg agaaaactaa tgcatctact
    10441 atgcagctgc tgaatgcagg gacaaaagaa tgggaaatag aacttcttga aaaaatcggt
    10501 gtctcgaagg aattatttcc accattgaca gagccgggaa cgatactagg agatctgcaa
    10561 aaagaacaat tttcaaaata tgatttgcca gatgtgacct ttatcacagt agcaagtcat
    10621 gatacggcgt cagctgtatt aggaacgcca ggtcaaggag aacgttgggg ttatatcagc
    10681 agtgggacat ggtcgctttt agggattgaa acaaagatcg ccaatgtttc tcgcaatgct
    10741 ttttctgaaa attatacgaa tgaatggggt gcccataaca cggtgcgttt tttgaaaaac
    10801 atcatgggga tgtggttgat ccaggaagtt gcccgaatgc aagagtacga gtacagttat
    10861 gcggaattgg cagagcttgc tgcaaaagaa aaaccgctgc agcaagtgat ggaaatcaat
    10921 gaccctcgat ttttgaatcc cccaaatatg atagaagaga ttcagcaata ctgttttgaa
    10981 acagagcagc cagtccctgt ctcatcggga gctatcgctc gttgtgtgta tgataatctt
    11041 gctctttgtt acgcacaaga gctgatcaaa gtagaacact taactggaac gaaattggaa
    11101 aagctgcaca tcgtaggggg cggatcgaat aatcgtctac tgaatcaatt gacagcagat
    11161 tatgccggaa tcattgtaga agcaggacct agtgaagcca cagcaatcgg gaatgttgcc
    11221 atgcaaatgc tcagtacagg tcagttttcg aatatagaac aagcaagaaa tgtaatcaaa
    11281 cagtcgtttc catgtgaaac tttttaccca gatgaaaata agcaaaacac gatggaatgg
    11341 tacgtgaata aaatcaaagg agtaatcgta tgaacacgat tgaaagaaat tacgaacaag
    11401 caaaagaaaa atatgcaact atcggtgtag atacagatgc tgtactagaa aagatacagg
    11461 acatcaaaat ttctatgcat tgctggcaag gagatgatgt caaaggtttc ttgacaccgg
    11521 atggcgaatt gaccggtgga atcatggcga cagggaattt tccaggagcc gcacgaacac
    11581 ctgaagagct tagacaagat ctggagaagg cttattcgtt gataccgggc aaacacaaat
    11641 tgaaccttca tgctatctat ttggatacag aagaatctgt cgatctaaat gaaattgagc
    11701 cgaaacattt tgaaaaatgg gtagagtggg caaaaaaaga agaaatcggt cttgacttca
    11761 atccgacttt cttttcgcat ccaatgatga aagacggctt tacattggct catccagata
    11821 aagaagttcg tgaatactgg atcgaacacg ggaaaagaag ccgcaaaatc gcggagtact
    11881 ttgggaaaga aactgggcaa acaagtatca ataacttttg ggtacctgat gggatgaaag
    11941 ataatccgat tgatcgtttt accccacggg aacgtttaat ggaatcactc gatgaaattt
    12001 ttacagaaga gttagatgaa cggtatgtac aagatgcagt agaaagcaaa ctatttggtt
    12061 taggcgctga agcttataca gtcggttccc atgaatttta tatgggttac gggttgactc
    12121 gtgacaaact tgtttgtcta gatgctggac attttcatcc aacagaagtg atttccaata
    12181 aattatcttc attagcttta ttcagcaaag gaatcatgtt gcatgtttcc cgtccagtgc
    12241 gttgggattc agaccatgtg gttttgatgg atgatgaatt gcaggaaatc gctaaagaat
    12301 tagtccgtaa cgaattgttg gaaaaaacaa atatcggtct ggatttcttt gatgcaacga
    12361 tcaaccgtat cgctgcttgg gtcatcggta cgagaaatac acaaaaagca ctgctgaaag
    12421 ctatgctgga accagttgag cgattgaaag aaatggagct ggcttttgac tttacttcaa
    12481 gaatggctta tacggaagaa ttgaaagatt tcccgtatgc cgatgtatgg aattatttct
    12541 gttacaaaaa tcaagttcca gttggtttgg attggctgga agaagtccag gaatatcaaa
    12601 aagacgtgtt ggaattaaga aaataggagt gtggctatat tgaaaaaaat agatatttta
    12661 caagcgcctt atgtgaaaga aatgatcgaa acgacgtcga atctttatcg cttgggatgg
    12721 gatgaaagaa atggcgggaa tatttcttat ttactaaaag aagaagagat cacaccgttc
    12781 ttggatcctg aaaaagtgat ccgccgcatc tcgatgattt ttgatgccag ccgtttagca
    12841 ggatgctatt ttattgtaac tggttcagga agatatttta aaaatgtagc gagcgaacct
    12901 gccgagaatc ttggaatcgt tcgagttgct gaagatggca aaacactgga acttttgtgg
    12961 gggctggaaa acgaatgcct tcctacaagc gagttgccaa gtcatttcat gagtcatatc
    13021 gcccgtttag aagtagaccc agataatcgg atcgttatgc attgccatgc tagtcatcta
    13081 ttagcgatga gttttacgca tgaattagac gaacgttcct ttagtcgtac gctatggcaa
    13141 atgtgtacgg aatgcttagt tgtttttcca gaaggaattt ccatcattcc gtggatggtt
    13201 ccgggaacaa atgagattgg ggaagcaaca gctgaaaaaa tgaaagaaac tcgattggtt
    13261 ttatggccac aacatggaat ctacgggtca ggaaaagaca tggatgaagt ctttggtttg
    13321 atcgagacag cagaaaaggc agcagaagtc tatacgtacg tcaaagcgca aggaccaatc
    13381 ttgcaaacaa tcacggacga aaacctgtgg catttagcag atgcgtttgg tgtcactcca
    13441 aaagcaggtt atttggagga agttcatacg aaagcaggtg tctgacatgg tgaaaaaagc
    13501 tttttgcatg aaagtttacc cagaaaaaca agaagaatac aaaaaaagac atgatgaact
    13561 ttggccagaa atgcgtacag cacttcgtga acacggggtc atcgactatt cgatctatct
    13621 tgatccgagt acaagtttgt tatttggtta cttagtgatc gaagatgaaa agttatggga
    13681 aaaaatggca aatacatcta tcaatcaaaa atggtggaag tacatggaag atatcatgga
    13741 gacaaatcca gatgcctctc cagtcagttg ggaattgatg ccagtctttg aactgtagca
    13801 acaagcaact gggcaaccgg ttgcttgggg ttctttcgtt tttattgtga aaaaaaatac
    13861 aaattaggag gaatagaaaa tgagtaatcg aatgatttta aatgaaacat cgtattttgg
    13921 aaaaggagcg attgagtcga tcgtccctga atttcagcag cgaggattcg gcaaagcagc
    13981 tgtcattact gacaaaggat tgatcgagca cggtatagca acaaaagtaa cagaacttct
    14041 agataaagca gcaatcgctt atgctttata cgacgggatc gtacctaatc caacgatcca
    14101 aaatgtaaaa gatggagtgg cttttgttaa agaggcagag gcagactgtt tgatcgcaat
    14161 cggtggaggt tcgccaatcg atacggcaaa agccattggg attattttga cgaatccaga
    14221 gttttcagac gtcattagtt tagaaggtgt tgctgatacg aaaaatccat gtttacctat
    14281 attagctata ccaacgactt ctggaacagc agcagaagta acgatcaact acgtcatcac
    14341 agatgaagta aatcaccgaa aatttgtttg tgttgatcct catgatatcc caatcgtcgc
    14401 gttcatcgac agcgatatga tgatgggaat gccgaaaaaa ttggctgcat caacaggtat
    14461 ggatgctatg acgcatgcta ttgaagggtt tatcacaaaa ggcgcttggg aaatgactga
    14521 tatgctgcat ttaaaagcaa tcgaaatcat cggccattct ttggaagttt ctgtagatgg
    14581 cgatcaaaat gggcgagaaa aaatggcatt aggacagtat attgcgggaa tgggcttctc
    14641 aaatgtcggt ttaggactgg tacacgggat ggctcacccg ttaagtgcat ggtacaacat
    14701 tcctcatggc gtagcgtgtg cagcactttt gccaactgtc atgaaatata acaaagaata
    14761 tactggagaa aagtaccgtg agatcgctct tgttctaggg attaaaggag cagctgaaat
    14821 gtctttagaa gatgtaagag aagcggcttg tggtgagatc gatcggttga gcaaagcggt
    14881 tggaatccca gaaaccattt ctgaattagg tgtaaaagaa gcagatattc cagcaattgc
    14941 agaagatgct ttgcgagatg tatgcacacc aggaaatcca agagaaacga cagtagaaga
    15001 aatcattgct ttatatcaaa gtttgatgta aaaacgaagc ggatcttcaa cattaaattg
    15061 cctgtaaaga gaaatggtag tttatatctg gatatctctt ttaaattgat ggtatttccg
    15121 ctataatttt tcttataaag aaataggaga atattatatg gaaaataaaa agccttatgg
    15181 aactgtattg atcaaagcct ctaaaatact agactattta gcggaaaacc aagatgtctc
    15241 tttgcagacg atcgcaaagg gtgtgggaat gacctcttct acagtcttga aaatactaga
    15301 tactttgttg atgatcggct acgtaaataa aaattcagag aaaaactatc gtttaggtgc
    15361 aaagcttatt cgctacgcga ataaaaacat tgaacaaatc gatttggtag agttgacttt
    15421 accgttttta gaacagctgc agcaaaagat cgatgagact atccacttag gggtattgga
    15481 caacaatgag atcttgtacg tgaacaaact agatccaaaa catcaaacga tccgcatgtc
    15541 ttcaaaaatc ggaatcaccc gaccattata caattctgcg atgggcaaag ccgtattagc
    15601 agaattcagt gaagagcagt acactgatta tcttgaaaag catccgttga tcccttacac
    15661 agaatatacg atcaccaatg cattacggtt gaaaaaggaa atccaaacgg taaaagaaac
    15721 ccacatcgca tttgacgatg aagaaatcga acgtgatatt ttttgtatag gagcctcaat
    15781 cgtgaaaaac gaacaaatca ttggtgcatt tagtgtaagt atgccgaaat atcgtttaac
    15841 agatgagaca aaagaacaaa taatcagtgc cctcaagcaa acaaaaagcg aaatcgagca
    15901 aagaattcaa aaataatgaa aaaagcgttt gcaatttttc ttttctgccg tataatatgg
    15961 gtatagattt ccaagttata aaataaaatt tctaaatagg aaataggtgg agaagatgaa
    16021 aaaaattgat attttatctc gattgaaaaa cgccggagtg attgcggtgg tacgcggaaa
    16081 aagcaaagaa gaagctttga atgcttgcca cgcaatcatc aaaggtggtc taactggtat
    16141 tgagttgact tttacagtcc ctcaagcaga tcaagtaatc aaagaacttt tgtcattcta
    16201 caaagaccaa ccagaaatag tgattggcgc aggcacagta ttagacgctg tcacagctcg
    16261 cttggctatc ttagctggcg cagagtatat tgttagccct tcctttgatc aagaaacggc
    16321 agagatgtgc aatctgtatc aaattcctta cttaccagga tgtatgacta ttaccgagat
    16381 caaaacagcg ctgaaaagcg gggtagatat cgtcaaacta tttcctggaa gcgcttatgg
    16441 accaagtatc atttctgcct tcaaggcgcc gatgcctcaa gtcaatatca tgccaactgg
    16501 cggtgtgagt ctggacaata tgaaagagtg gttcgatgct ggtgtcgtaa cagtcggagt
    16561 aggtgggaac cttttagcac ccgcagctac aggagatttt gacaaagtaa cggaagtcgc
    16621 gcaacagtat gcagcaaaaa tgaaagaaat caagaggtag ctgcttatgg gaaaagtagt
    16681 gacactcgga gaaatcatgt tgcgattgtc cacagagcag ggaacccgta tcgattcaag
    16741 tgaacgattc gctgcccatt atggcggagg ggaagcaaac gtagcaattt ctttagctaa
    16801 ttacggacat gaagcgattt ttgctacaaa agtacctgat aacgcattgg gaaaagcagt
    16861 caaaaagcac ttgcaatgtt atggagtaga tacgaaacag gtattatctg gtggatcaag
    16921 actaggtact tattatatgg aaactggtag tggcgaacgc gccgcctcag tgatctatga
    16981 tcgtgccgga tcaagttttg ctgaaatgga tcatttggaa tggtcgctag aggagttatt
    17041 ccaaggaata gatatatttc atgtttcagg aatcacccca gctttgtcta aaaaatggca
    17101 aaaaatgacg aaacaggtga tagaagctgc aaaaaaagct ggctgcctga tcagcttcga
    17161 tatcaactat cgtggaaaat tatggacaca gcaagaagcc agcgaagtaa tccatcagct
    17221 tttaccttta gtagatatct gctcagctgg aaaaatggat gctctttatt tgttaggtat
    17281 cgagaaagcg ccagaagcag aagaacaacc tctaatttat tattatcaaa aaatgcagga
    17341 aaagttcccg aatattcagg tgttttattc gaccaagcgt accgttcatt cagcaagtga
    17401 aaatgaattg aaggggacat tgtggatgaa ccatgaatat gtggaatccc aagtccatag
    17461 aatcacccca atcgtggatc gagtaggtgg cggagatgct tttgcaggtg gcgtgctcca
    17521 tgggatttta tccaatatgt ctccgcaaga aatcattgat tttgctacgg cagcttctgc
    17581 cttgaagcat acgatccatg gggactgcaa ccaatttagt caagaagaag tcgcacaatt
    17641 tttagcatgc ggctctggaa aaatcattcg atagaaggag tagaagaaaa tgcaagaaat
    17701 ggaaacacgt tatacacaca gtccagaaga tatccgtcat tattcaacgg aacaattgag
    17761 aaaagaattt ttagtagaaa aggtatttga accagggaaa atcagtttga cttatacgca
    17821 caacgatcga atgatctttg gtggagtgac acctactgaa aaagaattag aaatcacatt
    17881 agacaaagaa ctaggtgttg attacttctt agagcgtcgt gaactaggtg tgatcaatat
    17941 cggtggacca ggattcatcg agatcgatgg gcaaaaagaa gaaatgaaaa aacaagacgg
    18001 atactatatc ggaaaagaaa cacgacatgt tgtattttct tctcaagatg ctgaaaaccc
    18061 agctaaattc tatattagtt ctgttccagc tcatcataaa tatccaaatg taaaaatcag
    18121 tatcgaccaa gtaaaaccaa tggagactgg tgaaggcctg acactgaatg aacgtaaaat
    18181 ctaccaatac atccatccaa atgtatgcga aagctgtcaa ttacaaatgg gttatacgat
    18241 tttagaacca ggaagcgctt ggaatacgat gccttgtcat acacatgaac gtcgtatgga
    18301 agcttatgtc tactttgatt atgccaatga agatacacgt gtgttccaca tgatgggcaa
    18361 accagatgaa acaaaacatt tagttgtaga caatgaacaa gccattattt cgccaagctg
    18421 gtcgatccat tcaggtgtag gtacaagcaa ttactcattc atctgggcaa tgtgcggaga
    18481 aaatatcacc tatacagaca tggatatggt gcaaatggat caattaaaat aagcataagg
    18541 gagagaaaat gatggaattt agtatggata tgtttcgttt agatggaaaa gtagcattag
    18601 tcaccggtgc tgtctatggt attggatttg agattgcacg ctctttagct acagcaggag
    18661 caaaaatcgt atttaataac ttgaatcaag aatcagttga tgaaggtata gcgcattata
    18721 aagaagcagg catcgatgca aaaggttatg tctgcgatgt gaccgatgaa gaagcggttc
    18781 aagcgatggt tcaacagatc aaagaagatg taggttcagt tgatattcta gtcaacaatg
    18841 cagggatcat caaacggaca ccgatgatcg aaatggatgc ggcagacttc cgccaagtaa
    18901 tcgacgtgga tttaaatgcc ccattcatcg tttcaaaagc agttattccg gatatgatcg
    18961 aaaaaggcgg cggtaaaatc atcaatatct gctccatgat gagtgaatta ggacgcgaaa
    19021 cggtcagtgc ttatgctgcg gcaaaaggcg gattaaaaat gctgacgaaa aacatcgctt
    19081 ctgaatacgg tcaatacaat atccaatgta atggaattgg accaggttat attgctacac
    19141 cgcaaactgc tccattacgc gaaacacaag aaaacggtga acgtcatcca ttcgatcaat
    19201 ttatcgttgg acgtacgcca gcagcacgct ggggcgagcc aatcgatttg gctggaccat
    19261 ctgtcttctt ggcttctagc gcatctgatt tcgttaacgg ccatgtcttg tatgtggacg
    19321 gaggcatact tgcctatatc ggaaaacaac cttaaaagag ctttagtaca ctgtttagtg
    19381 gagaggttgt gaaatcagcg gattgaacga ataccgaagg acagctgatt aaacatcatt
    19441 tattcgaaaa aagaggctgg gacagaagca tttaactcca agaaataaga aggaattcac
    19501 gaaaattgct tttcaaattt ttgtgaattt cagcttattt ccgaaggagt tgcttttact
    19561 tccgccgttt gtacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc
    19621 tcttttctgt gcgtcttcca tacttcacta agtcgatccc tcttgcttcg caccaatcct
    19681 tcactggtcc ggaaaagatc agagattgtt ggtgtagggc cgcggtattc gttgcgccga
    19741 ccatagtata aagcagacga agctcttctt gccattgctt catcagtata atagtttctt
    19801 ctacgccgtg attcatcaga tgcgtcagta cggttcctga agtacccacg gcatttgcgc
    19861 cgagacaaag ggctttaaat atgtcatagg cattgcggat acctccggag gctaggattt
    19921 ccatagaagt gtctgcttca tttgcttcaa gcaatgaaga gactgtcgat tgcccccagt
    19981 cagctaaata actcagttcc cgcttggagc gacgggcgtt ttcgatctgg gtgaagcttg
    20041 ttccgcttcg accgctgata tcaactgtat gaactcctaa agcagctaga tcattgagcg
    20101 tttctcttgt catgccaaaa ccgacttctt ttacgatgag ggggacgtca atggctgttt
    20161 gagtttcttg aataagtact ttccaattgg taaaatcacg gtctccctca ggcataacca
    20221 actcttgagg tgcattcaaa tgaatctgta aggcatctgc gtgaaacaat cggatagctt
    20281 ctttggctct ttcgacagaa gtccctgcac cgatgttggc aatgattttc ccgtgaggat
    20341 attcttgtcg cataatcgta taagtatcgg ctaaagaagg gactttcaag gcagcgctga
    20401 ctgaaccagt ggcaatcatc aaatctgctt ctcttgcgac gattgcaaga tcttggttga
    20461 ttttttttgt tttttcgctt ccgccagtca tcgcattgat ataaaatggg ctgcttagtg
    20521 taaacccaac tgcctgtgta cttagatcga catcagaaac tgctatttgc ggcaaagggt
    20581 tatggatgat ccgtacgaag tcaaattcgt ttttttgttt atcatgaaag gctttagcta
    20641 gtgaaacatg ttcgtctttt cgattcatcg gttttccttt ctttgatcgc tatagacatg
    20701 taacggcagt ggagtgattt ctgctttttc ccatgcactc attaaaggaa gaatgccaga
    20761 tttctggtca acaattacga ttccgcaatc gcccccacct gcaccagaag attttgcggc
    20821 tccttcatac tgttcagcta aattacacaa tttgttcaaa gcaggcgttt cgatgacgac
    20881 cccagtgatt gcagataaat catgcagtaa ttgtcggttt tttcgaatca tctgttggat
    20941 caacgttaca ttattttctt taaacccttt gatcatttcg ttgacacatt ctgtactatt
    21001 ttttaagaaa agctgataag ccaccatttt atcttctctc gaacggtgaa cttgatcgac
    21061 caaatcagaa gtagaggcag ggctacccgt ccaaccaatc aataaacgta aatcttcagg
    21121 agcaatcaat ggctcaatgg atagacctgg ccaatccagg gcaagtaact cactgataga
    21181 atgctgagtt tcttgttctt ggagccaagg atggtcgaag gttgagaaag cgatccagcc
    21241 accataacag ctagcagcga tgtcgccgca ggaaccatta tcttgaactg ctaaattggc
    21301 tagtgctgca atcttgaaaa tctccaactg agataaattc aacgcgtaaa aaacattcaa
    21361 ggctttgacc gttgcaacgg tcacggcacc gctagaaccc aatccatatt tccggccatt
    21421 tgaactgtct aactcacttg ttactttcag atcataaaag gataaaagaa tgtttttttc
    21481 ttgtgcatac ttttcagtca agcgaatagc agcaaggata taatgaaaag gattttcccg
    21541 aatatctaaa actaattctc cgttgcgtct tgtccaacgt acaggcatcc cactatattg
    21601 tgcagattgg atacttccga cttttcgtgc agattctaca gtgactgtca cgaattgatc
    21661 gactgcagcg ataactgctg gatggcctgt ttcaacaact gcatattctc cggcaatata
    21721 gagtttgcct ggtgcagata cttcaatcat aaaaatctcc ctttattcaa taatcgcaat
    21781 tcctggacca gcatgtgcag taattacttg ttctttgcta aataatttgg taaatgtttc
    21841 ttgtacctca gataaatggc tgttttccac taaaactttg acattagggc cagcatccat
    21901 cgtaaagtag catggaatgc cttgctttct caattgtcgc accgcatcca tcgcttttaa
    21961 gctgtcagga gaccagtaag tgaatggagg ctgggcagct aaagtcgttc catgcatttt
    22021 tagtccgttt ctttccatcg tttctccgag aagttggaaa tcttttgttt tgattgcttg
    22081 tttcaattga tatagatccc cttcaacgga atctaaccag ccttgataaa aactggatgt
    22141 ttcgactgtc cgacgcatcc catttctgct ggacacttct tttttctggt cgttgatcaa
    22201 aacgaaaacc attgctaaat cgtcttcgaa agaatcggaa gggactggct tagcgtaaga
    22261 acttaagtcg tcatggcctt tttcccattc gacgaaacca ccgaaaatac ttcggcaagc
    22321 tgaaccagac ccgcgtcgag caaatcttga aagagagaga tcatctaatc caagcttaag
    22381 agcagtattg caagcgcctg ctaaagcagc tagcccgctg gcagaggagg ctaatccagc
    22441 tgcggtagga acgaaattct gactgattac tgaagctttt agtgagaggc cggcttcttt
    22501 acgaaaaagg tcaagaaact gactgacttt ttttgttgct ttttcgtctt gcaaggtgcc
    22561 atctagataa aattcatcta ccatatagct atcagaaaag atgacttctg tctctgtata
    22621 aaatgcatcc aatgttaacg aaaggctgtt gttcattgga aggatgagtt cttcattttt
    22681 cttaccccaa tatttgatta gagcaatgtt ggtatatgcg cgtgctttgc ctttaaacat
    22741 aggtatgtac tcctaatcct tgtatccaag tttcagcagc acccgcatct tcaagtgctt
    22801 gactgatttc ttgggctttt gtttttgttt gtgctaatgc aatcatacag ccaccgcgtc
    22861 caccgccagt taatttggca cccaatgccc cggtatcttt tgctgtttgg atcaatagat
    22921 taagaaaatc attgctgatt gtcagctttt ccagaagtga ttgtgattca tccattgtct
    22981 gtgccaacgt ttctggtgaa ttttcaatga ttgcttgttt tgcttgcttc gtcaagtatc
    23041 ctaatttttg gatcatttgt ccagtttcat gaggctgggt ttcaaagagg tgagcgacat
    23101 ctttgacggc ttctcttgtt tgtcctttga ttcctgtatc agcgacaatc aaaaaagcat
    23161 cgatgttcaa agaaaagtag tcgaaaggat gcccttttgt aaaataaatc ggctgcaagc
    23221 tgctagtagc ggctgcatcg attccactag gattaccgtg ggcgattttt tccgaaagct
    23281 ggacattttc taatagtatt tcacgagaca aaggaaatgc taagtaatca taaaaagcac
    23341 gagtgactgc tgtggcgact gcagcgcttg atcccattcc acgttcagca ggaatcgtgc
    23401 tttcaatggt cagttgcaag tttttttgga tgtcatgctg tttttttaag tgaatgaata
    23461 attttttgat attttttaat gcatgaggag cttcggctaa atttcctgaa tagtaggaag
    23521 aaaccagttg atcgtccatt gcatccagct cttcaaggaa tgcggtgact tttgttgcat
    23581 agaaaggaaa ggcaatcgct ggttctccat aaacaaccgc gtgctcgccc atcaatatga
    23641 tctttccgct tgattctcct tggccatagt ttgccataat ctaactcctt ctcacgtaaa
    23701 agtgattttt aagcaaaaaa taaagcgccc aaaacgagtt gcgctgcttc acgaattttt
    23761 ttgtccacta agggtctcaa aaaacattcc tcctttattt tactaaaacc atctcagaag
    23821 taaaactaag aatacgtttt ctaaagaaaa aacacttttt tcttaagaat tttttgactt
    23881 tttttcttct ttgactagaa aaaaatacaa ttgacactac tagatattgg gggtatattt
    23941 aaaaaggttg gagttaaaca caatatttag tgatgaagtg agctggaggt aaatgagcgt
    24001 gatcgtttta gcaggtacaa ttggtgccgg aaaatcaagt ttaacagaaa tgatggctga
    24061 acactttgat agtcaggcat tttatgaatc gattgatgat aatgaagtct tgcccttatt
    24121 ttatgcaaac cctgaacagt atgctttttt gctgcaaatt tattttttga ataaacgttt
    24181 tgcaagcatc aaacaagcaa tgaaagatga caataatgta ttggaccgtt cgatttatga
    24241 agattcactg cttttccatc taaatgcaga tctaggaaga gcaacagaaa cagaagttcg
    24301 tgtgtatgat gaactgctgg aaaacatgat ggaagaactt ccctatgctg cccataaaaa
    24361 acatccggat ttattagttc atattcgtgt atcttttgat acgatgttgg agcgtatcga
    24421 aaaacgtggt cgttcgtatg aacaattgtc ctttgatcca agtctatacg attattacaa
    24481 agaattgaac agacgttacg atcaatggta tgaagaatac aaagaaagtc caaaaatcca
    24541 aatcgatgga gaccgctata attttgtgga agatcctcaa gcaaaagaag aagtcttgaa
    24601 aatgatcgaa gaaaaattag cagagattcg ccaaaccaaa gtagccattt gatttacagt
    24661 tgctcaaata aagggtaaac ctttatttga gcgacttttt tctttatact ggaaacaaat
    24721 gtcaggagga tagagtatgc gggaaaaaca ctattttaat caagaccgta atttttggat
    24781 caatgtacag caaaacgatg tgctagctgt taaaaactta aaagaagaat atggcattag
    24841 tgacgagatg ctgacttatt ctctagataa aaatgaacgg gcgcgcgtag agtacgattc
    24901 ttttgatgaa gcgttactgc ttgtatcaaa tgttccgcac cagcaaaaag tcgataatca
    24961 ttatgaaact agtcctatcg cttttatttt aaaagaagac ggtttgttta catttaccac
    25021 gccgaatacc gagtacgtga ttcgactaat tcgatcgctt ttggaacgaa tgccagacat
    25081 gagtgtatac agtttattgt ttcgtacatt gtttttgatt tctgattcct tttttccttt
    25141 aatcgaggaa gtgaatagtg aacgtcagcg gcttaatctg aaattaagag aaaaaacaac
    25201 gaacaagaat ctgctgcaac tttcagattt agagatcggt ttggtttact tagttacagg
    25261 aacaaaacaa aatgtagtac ttttagaaca aatcaaggca ttggctattt atcgcaagtt
    25321 gtcagaaaag gaaaaagaag agttggacga tgcgctgatc gaagcacgac aagcagttga
    25381 aatgacgaac ttagcttcgc agattttaga ccagctctct ggcacttata ataatttgct
    25441 gaataacaac ttgaatgaca cgatgaaatt tttgacggtc tggtcgctga tcttgaccgt
    25501 tccaactatt gtgacaggat tcttcggaat gaatctccag ttacccttta cccattccgt
    25561 tttcggacgg ggaatcgcct tgatcatcag tttggtctta tctatttgga tgcttattgc
    25621 actgtggcgg cggattcgtt gatctagtct ttgatctgca aaccaagggc ttgggcgatc
    25681 acaagtgctt gctcttggtt gacgattagt cccgccaaag aatcttgaga taaagcgatt
    25741 gctgtgaaga aattagtcgt aaagtctaat cccttcagag aagttcgaaa ccagttcgaa
    25801 ccggtcaact gattgtcaga caaaaaaagg tttttccaag tgacctcata aaattcgctg
    25861 tcttctaatt gacaatgatc gaaggagaca gctttcaagt ttgtactact aaaggaagcc
    25921 atcgaagcaa tacaatcagt gaaagtacag tctcgaagat aactttcggc aaaattcgta
    25981 cctgttaatt tacattgatg aaacacgact tggtggaaac tgccaccaat ccactccaga
    26041 ttggaaaaat cacatttttc aaaaatgaca ttactgcatt caaaatgacg taaaactgtt
    26101 ttttgcatcg taagtttttg gatatgactc tctcttaata ccatattttt ggcatcgaga
    26161 tagctttggt cacccccgtt gatcagaaga ccagagaaga tctcctcatc ttctaaataa
    26221 aactctccct ttgtaagaag aggaagagaa ggtggaatag gttcgatttt tttcatgtgg
    26281 gttcacctcg tttttcttta gtatagcaaa gaagcgggaa tcactgctag aaagtctgct
    26341 aatctaatca ttttcagaaa aggaagacaa actgtatgaa aaacattgac aaaaagatta
    26401 gtttttgcta tactatgatg gttgagaaat aaaagcagaa gcacccgctt ctcgccttag
    26461 ttgataatgt cactgggcta gatagttttc actggttcta ttaggtgaag tacgcgcggg
    26521 ttctgtaaga gaactcgttt ttttattgcc gtttattcgg taagcttttc tttctctcat
    26581 aatatttgga ggtgaatgac catagcaaag gatatgatgg ttaacgacgg cattcgtgca
    26641 cgagagttac gattgatcgg ttcagatggt gagcaattag gagttaagac aaaagcagaa
    26701 gcattgaata ttgcagaaca agcaaacttg gatcttgtgt tagttgcccc tggtgcgaaa
    26761 ccaccagttg cgcgaatcat ggactacggt aaataccgtt tcgaacaaca aaagaaagac
    26821 cgcgaagcgc gtaaaaaaca aaaagtgatc aatgtaaaag aagttcgctt aagtcctacg
    26881 atcgacttga atgacttcaa tacaaaactt cgtaatgcac gtaagttctt ggaaaagggc
    26941 gataaagtga aagcttctat ccgtttcaaa ggccgtgcca ttacccataa agaaattggt
    27001 caaaaagtct tgaatcgctt agctgaagaa actgcggata ttgcaacagt ggaacaaaaa
    27061 gcgaaaatgg acggacgcag catgttcttg acgctggcac cgaaaaacga cagtaagtaa
    27121 tttgaatgac taactggctg ttggacagcc gatagtcgga taacgaaaga ttttgaggag
    27181 gaaatatagt catgccaaaa caaaaaacac accgcggatc agcaaaacgt ttcaaacgta
    27241 ctggtaaagg cggattgaaa cgcttccgcg cgtttacaag tcaccgtttc cacggcaaaa
    27301 caaaaaaaca acgccgtcaa ttgcgtaaag caagcatggt ttctagtggc gacttcaaac
    27361 gtattcgcca acaactagca aaaatgaaat aagaagtaca aataacagaa tctgaagatt
    27421 tatttttatt aggaggaatt atacatggca cgtgttaaag gtggaacagt aactcgtaaa
    27481 cgtcgtaaaa gagtgctaaa attagcaaaa ggttactacg gttcaaaaca cactttattt
    27541 aaaacagcaa aagaacaagt aatgaattca tacaactacg catatcgcga tcgtcgtcaa
    27601 aagaaacgcg acttccgtaa attgtggatt gctcgtatca acgcagcagc tcgtatgaat
    27661 ggcttgagct actcaaaatt aatgcacggt ttgaaagttg ctgaaatcga catcaaccgt
    27721 aaaatgttag ctgacttggc tgttaacgat gcagcagcat ttactgcatt ggctgaccaa
    27781 gcaaaagaag ctttggctaa ataagctaaa caattataaa gaggaaagaa gataacgaaa
    27841 agttatgttc tttcctcttt ttttatttac aatcctccat caaatgaaac aggacaataa
    27901 cgtataagtc taaaatagca taattaaggt tttttaattg aaaactgaac atttatggtc
    27961 accctttaat gaaagcgcta actacataag ttagaatgag tttgactgat atattagttt
    28021 gcttatcaga aaaacaaaat ggacggagga ataacatgag tgatattatt attggtattt
    28081 tgttagttat ctcattcttt ttcatggtct ggtactgtgt aaaaggctac aacttgatgg
    28141 tcggctttgc catcatggct actgtatgga tgggattagc gctagtcgga aacacgtttt
    28201 caccaaaccc ggcgatggaa gggcaaggag taattgatgt gttgacgcat atctatacga
    28261 cggggccagc tgagtacgcc aaatcgatct tggtaaatgt cttctttggt gcctttttcg
    28321 ggcgtgttct agtagatagc ggaatcgcag ctaccctgat tcgaaaggta gttgaacttg
    28381 gaggggacaa gccaagaatc acgatgagct tactatgtat tgtaaccgct gttatattca
    28441 tgtcgatgac aggaatcgga ccagtgattt ctattgcagt tattgtgttg ccaatcttga
    28501 tgtcattagg tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg
    28561 gaatctttgc taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc
    28621 cagcggctga aagctatact tataatgact atttccaaat tggtatgatt gggatgattg
    28681 taagcttagt tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg
    28741 caatggcagc aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg
    28801 tattattacc agttttaggg gtcgttcttt tagatcttcc aatcatctta ggttttattc
    28861 tagcaggtat ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct
    28921 gtcgtcagtt tgcgaaatta tttacagatg gcgcagtaga tgttgcacca atggttggtt
    28981 tcttgatgac cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag
    29041 ctatttttgg agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat
    29101 tgacaccact aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt
    29161 tagctgtcgt attagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg
    29221 caatcacaac gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat
    29281 taggttatac aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga
    29341 tcatcggagc gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa
    29401 aacgtcgata aaaacgatta gatgaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat
    29461 gggaattgat gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga
    29521 aatcatcgga aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagccgcagg
    29581 tgtcgttcag tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt
    29641 gtttgttgca cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa
    29701 agtcggcgta atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac
    29761 tagattgaac gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt
    29821 acctgtcaaa cacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga
    29881 aagagcggag gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg
    29941 agttgtttat gaagcagcat cggtaaaatc gatccctaca acgatggctt ctgacatcac
    30001 aaaattatat ggattgacaa gaagaactcg gacagcagca atcaatgcat ctattttacc
    30061 aaaaatgcta gatacggcaa cctctacaga agattccgta cgagaagcag gggtcaatgt
    30121 ttctttgatg attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg
    30181 acctgtttta accatgcttt ccggtccagc agcatcagtc atgggctctt tgatgtactt
    30241 gagagcgtct aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat
    30301 taaaaatgga cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc
    30361 ttcattagat gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc
    30421 aggaatcatc gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt
    30481 tacggagaca gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga
    30541 ccctgctgat tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac
    30601 atgtgcagcc aatgtccttg gattagtgca agaagaacat ttttcttatg gaaatgtccc
    30661 atctgcacga aaagccatcc aagcattagc tgattattgt catacgacgg ttgaagacat
    30721 cgcagaacag atcatggaaa aatcctatgc taagattgaa ccggtgattt tagaattggc
    30781 agacaagtat cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc
    30841 ctcattgatt acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc
    30901 cgaagtgatt tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat
    30961 catcccttcg ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc
    31021 gattgaatca ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacagac
    31081 ttcaaaagta acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa
    31141 ggagatcaca acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa
    31201 tgaagtttgc ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc
    31261 aaaaaatgct ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg
    31321 acatgcttca tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg
    31381 ggaagatatg gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt
    31441 aggagcaaga gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga
    31501 tctggaagta tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa
    31561 acaaacataa gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg
    31621 gattctacca gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa
    31681 ttcttataca acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt
    31741 acccttttgc cgatgtagga gaaatttgtg aacaaatggc tgttccaatc gtttcctgtg
    31801 aatcagagca aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggattc
    31861 gattgatgac ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag
    31921 aaacagcgca agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta
    31981 agggaattta tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc
    32041 aatcaaacat tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc
    32101 aaagcgattt ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc
    32161 gtagccaagg gatcttagaa gaaataaaaa aaatagaaaa aagatgtacg gagtatgatt
    32221 tagattacaa ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa
    32281 acgatgttct cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc
    32341 tgttttttga aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga
    32401 aaacggtgtt caaaagcttg catctgcggt aataagtcca gccaagttaa aaacataaga
    32461 agctattttt acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc
    32521 aataaacggt gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg
    32581 aggaactatt ttgccgtttc tttctctaat actcaggtca aaacgctgat tccaaaacct
    32641 cttatttgat gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt
    32701 attcttgctc ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa
    32761 agttatgatc aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt
    32821 ctaattctga aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt
    32881 ctagccaatc ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag
    32941 tatccaaatc aaaaaaagct tttttatttt gagcgattac tgcctgatcg gtttttccaa
    33001 cttttcccgc gtataacggg ctaatgaagc agatcggata tcgatttttg aaacataagt
    33061 gccggactgt ggaatggttt ttacaagctc ttgattgcgc agttggatca gtgcttctcg
    33121 aatcggtgta cgcccgatag ctagttgttc ttctaacact ttatcggaga ttttacttcc
    33181 aggtgctaac tcggaataaa tgatttgttt acgtatagat tcataagctt gctgttgcaa
    33241 attattgata accatttaac cttcctttct atgcagatat aatctattat aacataaatc
    33301 gcttacaaat cgtgtgggca taaaaacaca gtctgcaaaa aaagccctga taggcatgcc
    33361 tattagagcc tcgaaaagaa aaatattgaa tcaatctggt cgtacctctt gtatcggttt
    33421 gacacgaccg ccgattcttt tagcaaaagc ttcggattct tttttgtctt tgaaaatgag
    33481 cagattgtct aagttttttt cccgagtgcg acagccagtt ttatcgatgt aaccgtgcat
    33541 tactttaacg acatacatgg ggatcacctg cttttttctt ttcattatac gaactactcg
    33601 acacaataca agaaaaatgc ttagacggtc tttattaaga atttacatca tccaaagaag
    33661 cgtacgcttg gaataactga tctaactccg gattgacttt gctcagcttc actaatcgtt
    33721 gcgaatcaga aagtaaaaaa cagtgtttac tactgccagt agtttttaat tttttgtctt
    33781 cagttcccac aatttcgtag ctaaaatcca accgtgtccc cgtatacttt tggatagttg
    33841 gatggatgct cactttttct ccgtaatgga tcatttcttt atagctgcaa tttacttcca
    33901 gaacggggat gatgatccca gattcttcga tttttttata gggaaaccct agatgtacca
    33961 ataaggctac ccgagcctct tcaaaccagc ggatataatt gctgtgatga ataatcccca
    34021 tttgatctgt ttcataataa aaaggttctc gcacataacc tgtaaatttc tccatgactc
    34081 atgcctcgtt ttccattgat tttttacaaa aataattcat ccatttcgga agtgcttcgc
    34141 tgatttgtgt cggcaaaaca atatattgtt cttttgccag ctggtctcca atgaaactat
    34201 gaagataaat agctgctgca atcgtttggt cattttgagg aaactgggct aaaaatcctg
    34261 tgatcatccc agctaaagtg tcacccatgc cgcccgttgc catagcagca gaacctaaag
    34321 gattggtgta gaagtgctgg tgaccataaa tttttgtctc atgacttttc aaaacgagca
    34381 cagcaccgat ttcctgttgc ttctttttgt tttctttatc ccgttgttct tgtatcggta
    34441 gagaactcaa ccgttgccac tccatttgat gaggtgtaag tacagtttgt tcgggatagg
    34501 gtagcaaaag atgattagca gcaagtaaac ttaaagcaga gccatcaatc accagccatt
    34561 gttcttttga ttggttttct agtacttgtt tgaaacgttc caaactctta gaagaagtac
    34621 ccaatccggg accaatcaag agtacatctg cttgctttat tacggaaaga aactggtcag
    34681 tatccgtcca aactacaatc attgcttctg gcagatgact gtgaagagca ctccgatttt
    34741 tttcatcagt tactacagtc gtgagacccg ctccgctgtg tacggcagct aaagcactca
    34801 taatgattgc tccgccatat tgttcattgc cgccaatcaa aacgatacgt ccaaatgttc
    34861 ctttatgtga aatttttgga cgaggtgtga tgactctttt cactagttgt tcgtttatca
    34921 gcatctaaaa acctcctttt actcattata gcgtatattt tttttagaga aaaacagata
    34981 agaaaaggaa ttgtcagatt ttttgaacta tttttttctg taatttgtgg tatcatgaac
    35041 acgaagactt aaataagtgg aggattaact ttatgacaat tgattggcaa aaagaagtag
    35101 aagcacgtaa agaagacttg cttgaagact tgaaaaatct tttacgtgtc aacagcgagc
    35161 gcgatgactc aaaagtaaca ccagatgcac catttggacc tggacctcgc gacgcattga
    35221 aacatatgtt agcttatggc gaacgtgatg gatttaaagt aaaaaatgtc gataactatg
    35281 cagggcatat tgatcttggc gaaggagacg aaacgctagg gatatttgga cacatggatg
    35341 tcgttcctgc aggagatggc tgggacacag atccttatga accagtaatc aaggatggaa
    35401 agatctttgc acgcggatca agcgatgaca aaggtccaag tatggcagct tactatgcga
    35461 tgaagatcat taaagaactt gacttgaaac tatcaaaaaa agttcgcttc gtagtcggca
    35521 gcgatgaaga aagcggctgg ggcgatatgg cttattattt cgaacatgaa gaagaaccag
    35581 attttggttt ctcaccagat gctgaattcc caatcatcaa tggtgaaaaa ggaaatgttt
    35641 ctttagcttt acgcttcaaa ggcgacaatg caggagatta tgtcttgaaa tcttttgttt
    35701 ccggacttcg cgaaaatatg gtgccaggaa cagcaactgc agcacttcaa gtaccaagtg
    35761 cagatgcagc tatcgcgatg gaagaagcat tttaccaatt catcgaagca aatccagtaa
    35821 gcggaacgat tgaagcggac aatacgtatg taaaaatcga attagttggt aaaggcgcac
    35881 atggtgcaag cccacaatct ggtatcaatg caggttcttt cttggcattg ttcttagata
    35941 actatgaatt cctaggatct gctaaacaat ttatccatgt agcagctgct tatgtccatg
    36001 aagattttta tggagaaaaa ctaggagtcg cttatgaaga tgaaaaaatg ggcaaattga
    36061 caatgaatgc tggactattt gcttttgaag aaaatggtac agaggaagcg aacttcatca
    36121 atatgaattt ccgttttcca aaaggtgtaa cagtagacgg attgcaaagt gacatcgaac
    36181 aaacagtggg gcaagaaggc gcaacagtta ctcgaggtgc tcgcgtaatg gaaccacatt
    36241 acgtaccaat ggatgatcca ttggtggcta cattattgca agtctatgaa gaccacactg
    36301 gagaaaaagg ctacgaacaa atcatcggtg gcggaactta cggacgtttg ttgaaacgtg
    36361 gcgttgctta tggagcgatg ttcccaggat atactgatac aatgcatcaa gcaaacgaat
    36421 ttatgagtct tgatgacttg ttcagagcaa cagcaatcta tgcagatgcc atctatcgtt
    36481 tggcgaaata attaattaaa aagaggatca agaattgcaa attatttttg cagtcttgat
    36541 ccttttttta ctttctactg tggtattcat gataaacatg atatagattc aattcatatt
    36601 ctttcttagc gttcgtgacg actgtatcca aaatcaaccc tgttactaat gaaagaagtg
    36661 ctaaagtcat caatccagta cttaaaatcg cagaagggat gcgtgtaata aagcttgtca
    36721 gcatatattc gcgaatcaca ggaatccccg tgaccaatcc aaataaaaag aagaagatgg
    36781 tccaaatccc aaagaaaagc aaaggcttat aatctttaaa cattttaata atcatcatga
    36841 tcactttgaa tccgtcagag aatgtattca attttgattc gctgccttca ggacgatcac
    36901 gataatcgat tgggatttca acgaacttga attttttatc taaagcatgg atcgtcaatt
    36961 ccgtttcgat ttgaaaacca gaactcataa taggaaaact tttgacaaag atacgattga
    37021 atcctcgata gccggtcatg acatcaagaa tttttgtttt atacattcta gtaattgtat
    37081 ttctaacaag gttatttcca aaatcgtgga aaggtctctt attctcatca aagtaggttc
    37141 catttgatag gcgatcacca atgaccatat ctgcttcacc agaacgtaat ttctcgagca
    37201 aaccatgtac agcttctgca ggataagtat catcgccatc taccattaaa taatagtcgg
    37261 catcgatatc aaagaacatt tggcggatca catttccttt tccttgacga ggttcttttt
    37321 ttacgattgc tccaccttga acggccaatt cgtatgttcg atcggtcgaa ttattatcat
    37381 agacataaat atctgcttct ggtaattctc gtttaaagtc tgcaatgact gttgaaattg
    37441 ttgcttcctc attgtaacaa ggaatcagaa cagcaatttt ttcttgttgt tccatttttc
    37501 actctcctta actatagaaa acaaaagcta aattaaaaat acctcataat caaaggaaat
    37561 gctagttttt cccgctaaat atcatttatt taaaaagaaa atactggaat atatgtttgg
    37621 aaagctctat ctaaaaaacc acccactaca aaagaaggac atgatacctc tcttttgaat
    37681 agtgggtgcg tattcgttct ctcacttttt tgacgtatta atctgttgca cctttttttc
    37741 ttgaaatttc taatacggtt cctgtatgag catctgctaa aaattcataa taaacagttt
    37801 cgccatcttc tattcgagaa agacctcccc gataagcact cgaccgaacg gcgaatttcc
    37861 taataggttc cttttcaaaa gagatccaag aaccttcgat tgcaccttct tttagaaaag
    37921 atgacttgat attttcaagt acttggtctg ctgacaatac ttttttgcga tgataccaaa
    37981 tcgttgaagc taccccgctc aataatccta gaccgattcc aactgctaac ccgcctttaa
    38041 aatagcttag ttcgctttct tcatacatgt ggacatacct ccttcttctc acttgttact
    38101 ccttgtgctt attttaccac actctcatat tttctttgaa ataaataacc gctaccagaa
    38161 aaaataagct ataataaata cgatcggtct gcagaaaaaa tcatatttta tccgcagatc
    38221 aatataagtc tgagaggagt ttagtctgaa atttagacga atagcatatg gaagaaaaaa
    38281 catttcaacg aatcaaagag ttaacagaat tacaaggaac aagcggtttt gaacaagatg
    38341 ttcgcgctta tatggaaagt catatggaac cgctggtaga tgagttacag ttagatggtt
    38401 tgggcggcat ttttggtctg cgccatcatg aagaagcgga tgcaccccgt gtaatggtcg
    38461 cagcacatat ggatgaagtc ggattcatgc tgacacaaat ccaagaaaac gggcttttta
    38521 aagtcgtacc tctaggcggc tggaatcctt acgttgtttc tgctcagcgt tttacactta
    38581 aaacctctac tggtaagaat tatccatgta tttcttcttc cgttccccca catttattgc
    38641 gaggaacaag cggacaaaaa tcagtagaag taaccgatat tttgttcgat gctgggtttg
    38701 aatcaagaga agaggcaatg agttacggtg tccttccagg agacacaatc gttccttacg
    38761 ctgaaacgat caaaacagcc aacggaaaga atattatcag taaatcatgg gacaaccgct
    38821 atggctgtac aatggtatta gaagctttag aagcgttgca aaatgaaaca ttaggtcata
    38881 cattgattgc aggcgcgaat gtccaggaag aagtaggctt gcgtggttct aaagcttctg
    38941 tcaataaatt caagccagat ctgttctttg cagtagactg ctctgcagca gatgatacag
    39001 tgacgaaaaa tggcacattc ggacatttag gagaaggtac attgatgcgt atccaagatc
    39061 caggtttgat catgcttcct cgtttgcgcg agtacttgct ggatatcgct gaaacaaaca
    39121 atatccctta tcaatacttt gtttcaaaag gcggtacaga tgcaggtgct gctcatacac
    39181 aaaacgaagg aatcccaagt acagtgattg gcgtagtggg acgttatatc catacgcatc
    39241 aaacgatgtt cagtattcgc gattttgaag ctgcacgaga aatgttgatt caaacactga
    39301 aaggtttgga taaatcaaca gtgaatacga tcgtatacgg aaaataatgc gatagtgaaa
    39361 ctataaaatc tggcaaaaga ttgctttttg ccagattttt tttataaaaa gaggatcaat
    39421 gtttgttagt tcgaaagaaa ggtgtataat ttaattgaaa aatacataaa cagaaagtag
    39481 aggacaaaga atgattatcc caaatagttt agaagaatta gcaggctacg tagaaactgg
    39541 aaagagtgta tttttcttta ctgccggctg gtgcggagac tgccgtttta tcaaaccgca
    39601 aatgcctgaa attgaaaatg acttttctga ttggcaattt attgaagtag accgtgacaa
    39661 gtacatagat gtagccgctg aatggaacat ttttggtatc ccaagttttg tcgtgatcca
    39721 agacggaaaa gaattaggtc gtctagtgaa taaagatcgt aaaacaaaac aggaaatcga
    39781 atcgttccta gaaagtgtaa caaaaggctg acatctgtta ggaggggtca agatgaatca
    39841 agaatataag aatattttag taggtatcga cggtagtgaa caagcacttg ctgctttcaa
    39901 aaaagcggta gaagtggctc gccgtaataa tgggaaagta tatgtagcaa acgtaatcga
    39961 tcagcaattt tataacttta tgggctatgc acctattgat caaaatctag tcgatcagca
    40021 aactgaaaca gccaaggaaa tgatcgaaga atgtaaagaa tatgggaaat ctgttgatta
    40081 cacaaatata gaaggaatta ttgcatatgg ttctgttcgt gaatccatgg cagtcaaatt
    40141 gccaaaaaaa tatgagatcg atctaatcat ggttgggcag tcaggtctaa atgcagtcga
    40201 acgctttatg acaggaagtg tagccagcta cgtgatacgt cattcacctt gtgatgtctt
    40261 gatcgtttct gacgaagaag aacagccaag taattaagca agtggaactt gtacgacctt
    40321 tttaatcaat taactaaaac agtaaaggga ctgtgacaaa agtcttgtca cagtcccttg
    40381 ttacgaataa atggtgttcc agaagaggct tcttcggaaa taaagcatga aaaaaatttc
    40441 atgcagtgaa tgcaattttt tgttaagata aatctcagca aaggataatg gaggaaagaa
    40501 catgattttt gcttataaca aagcacacgt cggagacacc ttgatggtta ttatagccga
    40561 cgataaagga acagaaaaca ctgttgaacg caaatggaat gttgctcgga tcaaagatga
    40621 acaaggtcaa gttgttgctt ggaacttttt ccatatctcc gatcatctaa caatcgaagg
    40681 gaacggccaa gtaagcttaa cagatgagga attagcgaaa ttgaatcgat tgatcaaaga
    40741 agcaggattc gaagaacagc ttgaagctga tcatgaaccg aagatcgttg tcgggtacgt
    40801 aaaaacatgc gtcccacatc ctgattctga ccatttatcg attactgaga cagaagtaga
    40861 caatggtaca gtattgcaaa tcgtttgcgg tgcacccaat atcgaagctg gacaaaaagt
    40921 tgtcgttgca aaaccaggag ctatgatgcc agatggaatg atgatctggc caggaacttt
    40981 gcgaggggta gatagctatg gaatgatctg ttccgctcgt gaattgcatt tgccaaatgc
    41041 tccagcaaaa aaaggaatct tagtattgcc tgaggatgcc gtagtcggag aagcatttcc
    41101 aaaataaaga ggttgtgaca agtctttgtc acaacctctt ttttattaat tttcactatt
    41161 ttgattttgt tttaatgccg attgatcgat cgtcaattca acagtagttg ttttttcttt
    41221 cgagccttca tagtaagtga ttttgacaga atctcccact tttttcttat acaaagcaga
    41281 ttgaagttct acaccagaag aaacttcttt gtcatcgatc ttagtaatga cgtcgtattg
    41341 tttcaatcca gctttttcag ccggtgtagc tgtttggaca tttgtcacga tgacaccgtt
    41401 agttacagat gaaggaatct tcaagatttg ttcttgttgc tgagtagaaa cggcagataa
    41461 gtcgaccatt gtgataccta gagctggacg tgtcactttt ccgtcttttt ccagttgatt
    41521 gatgatattc actacatcgt tacttggaat agcaaatccc attccttcta cgcttacatt
    41581 agaagtagat tcagaagtac ttgcgatctt acttgagttg ataccgataa cttgtccttc
    41641 gatattaact aaaggaccac cagaatttcc agggttgatt gctgcatctg tttggatcgc
    41701 attgatattg actgtttcgt tcgattcatt cgtacttgtc acttgtcggt tcaaggaaga
    41761 aatgattcct gaagtgacag aatttgcata ttcagatcct agtggggaac caatcgcaat
    41821 cgcaggctca ccaaccttca gagcacttga gtcaccaaaa gaggcaactg tttcgacttt
    41881 gtctgaattg atcttcaaca ccgctaaatc tgaataagca tctgttccaa ctaattcagc
    41941 ttttaccttc gtaccgtctt tcatcaagac ttcaagacct tgctggccat ctactacgtg
    42001 attattcgtg actacataag cagtattccc gtcttttttg tagatcacgc cgcttccttc
    42061 actataagct tccaaattac tatcatctga gctgctactt tcttcttgct gcccgaatag
    42121 tccgccaaaa ccgcttgatt ggttttgact ttgtaagttg ataacagaaa cgacagcgtc
    42181 ttgaacttta tctaccgctt tggtgatatc agaatctaca ttgactttga cattttccac
    42241 aactgtttca ccggcagaat tctgattgcc gcttgtagca gtagaactat tgttgccagt
    42301 gcccatcgct gcataaaaaa tgccagctgt cagtagtcct ccaacgatcc ctccaacaag
    42361 ccccagacct aacttacgcc agaggctatt atttttactt tttttcatct ttggtgtcac
    42421 attttttcta tccatgtgtt cctcctccaa ttacattcat ttatctactg ttagtatagt
    42481 cttatttgtt gactttagtc tactttgaga ctgtgaactt tttatgaaaa actttaggaa
    42541 aaatcattgt tttcagatga aaatatttga ttttcttttt ccacagatac tgtgtaaaac
    42601 tacgcggata ggattggaaa aataagggat aaaacatatc cacagcctgt gtataaagtg
    42661 gataaattct tataaaacgt atgtttgcct aatgaatgtg atgaaataag gtaaaataac
    42721 actgttttct gtggataaac ctgtggatgt tgtgcataac tgttggggaa atctttttaa
    42781 caaaaatagc caaaataaac atatagacga aaaaattgat tataataaac aaagtgagca
    42841 tgaagaccaa aggagagaaa acatgaatat caaaattatc tcagtaggaa aattaaaaga
    42901 aaaatacctg atccaaggta tcaatgagta tgtcaaacgg ctgaatgcct atgcaaaaat
    42961 cgaattgatc gaagttcctg acgaaaaagc cccggagaat ttaagcgaag cgcaaatgag
    43021 acaagtaaaa gaaaaagaag gggaacggat cttagcgaaa atcaaagaac aagaatacgt
    43081 ctatgcctta gcaattgaag ggaaaaatcc tacaagtgaa tcttttgcta aacaaatcga
    43141 ccaattaggt atccaaggaa aaagccacct tgtttttgtt atcggcggct ctttaggact
    43201 cagcgaagcc gtcatgaaaa gaagcaatgc tcagatctct tttggaaaaa tgacgtatcc
    43261 acatcaattg atgcgtctga tccttgtcga gcaaatctac cgtgccttcc ggatcaatgc
    43321 gggggcgcct tatcataagt gatgcggttt ttatcattga tcctaaaaat cttagcaaga
    43381 caaaaagtac gtaagaaagt aaagactaaa tacttcggag gaacaaatga ataacaatga
    43441 taggttattg cgattgcgtt atgcgattga tttgaaagat acggatttga ttaaagcttt
    43501 tgagctaggc ggtgtaccac tgactaaaga agaagctcag gcggtactga ccaaagtaca
    43561 ggataagaac aaagataatg cagaaaataa tgtgtatgaa aaaaccatca acaaccaagt
    43621 ttttgacgca ttcctaaatg gcctgattct cttggcgcgt ggtccgcaaa aagataagcc
    43681 gatagtagac acgccgcaaa aaagcaagga aatcaaatat atcaacaatg tgttgctaaa
    43741 aaaattaaaa atcgccttgt ctatgaccac tgatgatatt ttagacgttt ttgctgaggc
    43801 agaaatctat ccatcaaaag gcgaaatcgg ggcatttttg agaaaagaag gccaacgaaa
    43861 ttttaagcct tgcggagata aatatatgcg taattttctc aaggggttag gaatttataa
    43921 tcggagaaaa gtataaagaa atttcatgag aaatcaaaac aatggttgtt ttgatgagag
    43981 ctagtttttg tatgagatta aaatattatc ttaatcgagg agctggattg gttttatgaa
    44041 tgatgaatat aagaatgatg aagataagat gctttttgaa gagatagaga atcgatgtag
    44101 gctcaatttt gagcttcggg gaaagatgtc tttaatacaa caaaaaaagt atttagcgaa
    44161 taaatcagag tttactctgg gacatgttga aaaattaatc tcggattgga ttagtagtag
    44221 aagcgaattt acaaaaataa aacagccgat taaatttgac atgaaaaaac ttttgttaaa
    44281 taagagtgag ataggtaata gagatcaata tattagagct aaagggcaag aaataattga
    44341 ttcattaggt gaaatgaggt catacaatta tttgtatgta acacatagag ctgatggaat
    44401 ggtcattact gtggggaaat cttcttctaa tgatattttt ttagatggag atctttttta
    44461 tcaactcaat acaaatcact tgtctggcac tgaaaatata attttaagga cggaatatgg
    44521 caatgaaata tttgcaaaat atgatgagat tttaaaaaat tatttggact gggcttggat
    44581 aattccggtt gagtcaggag atgctaagaa actggagaga ttattgggcg atgaactaat
    44641 taataaaaaa gtaccaattt taaattatta ttcccataga caataaaagg ctatttttca
    44701 tgataatact agttataact gtaaaaatgg aagaaaatct gggaaagaag tttttaaata
    44761 agtgtattgt tcgattttaa taaataataa gcaataccat ccgtctgtgt tgcgcaacat
    44821 cgtactacaa tgaatatttt ttcacaaaaa gaaaatcatc catcgaatga atggaaggtt
    44881 ttagtataca cgaggttaat gccccctctt ttataatcag aggagtgagg ttttttctat
    44941 gaatgtttct attttcttat tgctagtcta gtttctctta cgatgaaatt gggattctat
    45001 tccagttttt attgatttgc tgataaaata acttgaatgg tggatataaa catgataaat
    45061 aagagaagaa taacaaaaca aggttgtgaa atgattctca aatgaatata atagaaagaa
    45121 aaaggaggaa gaatcaatga aaatagcaat tgcaggcgct ggcgcaatgg gaagtcgctt
    45181 tgggttaatg cttcaccaaa gtgggaacga agtattatta atcgatggct gggcagaaca
    45241 tgtccaacaa atcaaagagc atggattaca agcaaatttt aatggtaaag aggtagaagc
    45301 aaaactacca atcgttcttc aatccgaagt agaaaaagaa gatcaagttg atctgattat
    45361 tctatttacc aaagcgatgc agctggaaaa aatgctgcag gatatccaat cattaatcaa
    45421 aaaagataca gaggtcttat gtctattaaa tggtatcggg catgaagata ttattgagaa
    45481 atttgtacca atggaaaata tctatattgg aaataccatg tggacggcag gtcttgaagg
    45541 tcctggtcag gtcaaattat ttggaagtgg ttcagtagaa ttacaaaatt taggtgatgg
    45601 aaaagaagca gctgcaaaaa aattagcaga caaattgtct gaatcaggat taaatgctca
    45661 tttttctgac aatattcact attctattta tcgcaaagca tgtgtgaacg gaacaatgaa
    45721 cggactatgc acaatcttag acgttaatat ggctgagcta ggaaaaacat cgactgctca
    45781 taaaatggtg gcgacgattg tcaatgagtt tgccaaagta gcagcagtag agaagattga
    45841 actagatgtc ccagaagtca ttgcacattg tgaatcttgt tttgacccgg aaacaattgg
    45901 gttacattat ccttcaatgt atcaagactt gattaagaac catcgattaa cagaaatcga
    45961 ttatatcaat ggcgcaattt ctagaaaagg gaagaaatat ggtgttgcga cgccttattg
    46021 tgatttcttg actgagcttg tccatgcaaa agaagatagt ttgaacgtaa aataaaggag
    46081 agattgaatg gaaacttcac aaaaaatatc accgaaaatt tttctgaata aagtcctcgc
    46141 tggtacagca acaggaatca ttattggatt gattcctaat gcagtattag gtgccatttt
    46201 aaaatatttt agtgcgtatc cctttgcggt aacgatcgca cagttagcag ttatttttca
    46261 actagcaaca cctttgatca ttggtggttt gattgcctta caatttggat ttgatccgat
    46321 gagaatgatg gttgtagccg gagcttcttt tgttggttct ggtgtagtca aattcacacc
    46381 tgatttgggt gaaacgggtg tttacgttgg tgctggtaca ggcgatatta ttaatacgat
    46441 gctgacagca tcgattgctg tcgggctgat tttactgatt ggaaatagtt ttgggtcagt
    46501 gtcgattgtg ttaacaccga ttgttgtagg tgtaggtgca ggtttacttg gtttttattt
    46561 attccctttt gtaacagcaa ttacttctgc aattggatca ttgattaata acttcactac
    46621 cttacaaccg ctgttaatgt ctatcttgat tggttgttcc tttgcctttt tgattatctc
    46681 tcctatttca acagtagcaa tcggaatggc aatccaacta gatggtgttt ctgctggtgc
    46741 ggctgcgatg ggcgtagctg caaccacttt tgttttagta gtgaactctt ggaaggttaa
    46801 taagtcaggt gtgacaattg cgattgcttt aggcgctatg aagatgatga tgcctaatct
    46861 atttagaaaa cctgtgattc ttattccttg tttagttagt gcagtgatta cagctattcc
    46921 tgtcgctcta ttttctattt caggtacgcc tgcttctgct ggtttcggcg tggtcggact
    46981 tgttggtccg ttggcttctt tagatgcagg gctaaatggc tttcttgttg ttctttgttg
    47041 gttagtcatc cctgtttttg cggcactaat tactcaattt ttatgcgaga aagtatttaa
    47101 actgtatgat cgagaagaag tcttcaaatt tttaggctag tagattttac taagaaattt
    47161 tcaatcaatg gatgcgtaag tgagatgtca acaacatctg acttacgttt ttttgccttt
    47221 ttgagtagga gattgatgct gacaactgaa ctcttcagtg ccattcgatt ttttaaaatg
    47281 tagaagaaac tcacctttat gttattgatt gttcaaaagg caactagaat aaaaatatgt
    47341 gttaaaatga gaaaaaattt gattgttgga tgtatcgaat aatcactaaa aagtaggaga
    47401 agaaaatgat ttttttgcaa aaatatggtt tttatttttt attattaggt gtgattagtg
    47461 atttatcgac accgtatatc cttggccttt tttatcccaa actgaatcaa atgactaccg
    47521 taattagtgt gtttggtgac gtagatagtc cagtcagacg tgctttcttg gtctggtcag
    47581 ttgtatctgg actttttttt gttctttcgt tgcctgcgtt gtatcaccta tttattggaa
    47641 catcaaaaac acttgcaata cttgctgttg caacagttgg tttatatggg ataggtgatt
    47701 gtatttttac tgggttattc agtatcaata cgaatgaaag ctcttggaat ctatcgactt
    47761 ggatccataa tataggttct gggctaggct atgcaggctt tctacttttc ccattgttac
    47821 ttgttctgct ttatcgtcaa agtggtcaag gtacgcttag ccatttttat cttgttttaa
    47881 cagttattag tttactaatt gcaggtcttt acggattagc acggattccg tcgattagtc
    47941 agtttgcctt ctttaagcaa ttgggcttct ttcaacgctt aagttttttc tttaactatt
    48001 tgccgatgat ttgtttaagt ttttttcaac tacgaaagta gaaaaagcag tgtgaagctg
    48061 attaagtaaa agaatttctc tatgggtaac cattcgtgtg gatgtgttcg actaagaata
    48121 gagatgagga gagcgaaaag caagttagtc ttaacttgtt atgcgttgtg ctaaatttca
    48181 ggtagtcatt ttttgaatga ataagaagaa aacttgaaag agggtgactt catgttagat
    48241 gtggagcaga tggaaataga cctttataat attattttaa atcgaaatac aagagaatgg
    48301 gaaagaacac aatttgaaga ggcaaaagaa gacctagcaa aaatgtcgat aaagcaggtg
    48361 atagaaaaac tagaggtcaa cttgcgtccg ctagcactac gagataattt gacaccagat
    48421 gcgatggatt tttatttaag aatcacaggg gagtcagcga gtgcaaaggc ttatgattat
    48481 gccaaacata agataacttc acccttatac caagaacggg cgatttttgc cggtggctgt
    48541 ttctggtgta tggttgagcc ttttgaaaca aaaccaggtg taatctcagt gttatctggt
    48601 tatacaggtg gagagaccaa aaaaccaaat tacgatcaag tcagtggtgg atatactggg
    48661 catgttgaag ctgttgagat catatttgat acacgtcaaa taacctatga agagctgttg
    48721 gagatttatt ggcaaatttc agaccctaca gatgcctttg gtcaatttca agatcggggg
    48781 aaacaatatc gttcaattct atttgttgtt aatgaaaagc aaaggcaatt ggctgaaaaa
    48841 tcaagagaac aactgattca atctaaccga tttagccgtc ctatcgttac agagattcgc
    48901 caagcaaaga ctttttggcc agctgaaaat catcatcaac aattttatca gaagcaacct
    48961 aaaagatata aaaagatcaa gcattcgcat cagcttcatt tattttttca gcgacataga
    49021 tagaggaagg ttgtggcgca aagcgttcag ctccgacaaa ataatgagca actgacaaaa
    49081 atagcttttc atatttttgg acagttatga attatttcga ggagttgctt tgagaacacc
    49141 gaggaaaagg ttgtggcgca aagcattcag cttcaagtcg caaggaaaca ggaggtatga
    49201 caagtctttt gtcacaactc ctgttttttt actagccttt atttccatgt gaaagagaat
    49261 tcgaattgta gaatcttgat ttttctatgg aattttgtag agaatcatct tgataattaa
    49321 tagacaccta atcgaattcc acattaatga tgaactaaat tccgtataaa agcgcaaaca
    49381 ataacta
//
LOCUS       DAZKOR010000011        47050 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000011 DAZKOR010000000
VERSION     DAZKOR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.011"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..1139
                     /locus_tag="ACG8ZM_000570"
     CDS             69..1139
                     /locus_tag="ACG8ZM_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0491558.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1154..1822
                     /locus_tag="ACG8ZM_000571"
     CDS             1154..1822
                     /locus_tag="ACG8ZM_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491559.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1842..2420
                     /locus_tag="ACG8ZM_000572"
     CDS             1842..2420
                     /locus_tag="ACG8ZM_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0491560.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2821..3732
                     /locus_tag="ACG8ZM_000573"
     CDS             2821..3732
                     /locus_tag="ACG8ZM_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0491561.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(3820..4737)
                     /gene="rnhC"
                     /locus_tag="ACG8ZM_000574"
     CDS             complement(3820..4737)
                     /gene="rnhC"
                     /locus_tag="ACG8ZM_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0491562.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            4939..5376
                     /gene="zapA"
                     /locus_tag="ACG8ZM_000575"
     CDS             4939..5376
                     /gene="zapA"
                     /locus_tag="ACG8ZM_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0491563.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5435..5983
                     /locus_tag="ACG8ZM_000576"
     CDS             5435..5983
                     /locus_tag="ACG8ZM_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0491564.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6098..8458
                     /locus_tag="ACG8ZM_000577"
     CDS             6098..8458
                     /locus_tag="ACG8ZM_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0491565.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8545..8859
                     /gene="trxA"
                     /locus_tag="ACG8ZM_000578"
     CDS             8545..8859
                     /gene="trxA"
                     /locus_tag="ACG8ZM_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0491566.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9041..10819
                     /gene="uvrC"
                     /locus_tag="ACG8ZM_000579"
     CDS             9041..10819
                     /gene="uvrC"
                     /locus_tag="ACG8ZM_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0491567.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            10964..11863
                     /locus_tag="ACG8ZM_000580"
     CDS             10964..11863
                     /locus_tag="ACG8ZM_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491568.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            11856..13118
                     /locus_tag="ACG8ZM_000581"
     CDS             11856..13118
                     /locus_tag="ACG8ZM_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0491569.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13279..15177
                     /locus_tag="ACG8ZM_000582"
     CDS             13279..15177
                     /locus_tag="ACG8ZM_000582"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0491570.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(15507..16226)
                     /locus_tag="ACG8ZM_000583"
     CDS             complement(15507..16226)
                     /locus_tag="ACG8ZM_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0491571.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    16416..16463
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            16563..16895
                     /locus_tag="ACG8ZM_000584"
     CDS             16563..16895
                     /locus_tag="ACG8ZM_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491572.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            16882..18885
                     /locus_tag="ACG8ZM_000585"
     CDS             16882..18885
                     /locus_tag="ACG8ZM_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0491573.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            18904..19374
                     /locus_tag="ACG8ZM_000586"
     CDS             18904..19374
                     /locus_tag="ACG8ZM_000586"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0491574.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19395..19979
                     /locus_tag="ACG8ZM_000587"
     CDS             19395..19979
                     /locus_tag="ACG8ZM_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0491575.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            19994..20980
                     /locus_tag="ACG8ZM_000588"
     CDS             19994..20980
                     /locus_tag="ACG8ZM_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0491576.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            20970..21281
                     /locus_tag="ACG8ZM_000589"
     CDS             20970..21281
                     /locus_tag="ACG8ZM_000589"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0491577.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21303..23084
                     /locus_tag="ACG8ZM_000590"
     CDS             21303..23084
                     /locus_tag="ACG8ZM_000590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0491578.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23077..24453
                     /locus_tag="ACG8ZM_000591"
     CDS             23077..24453
                     /locus_tag="ACG8ZM_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0491579.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24460..25095
                     /locus_tag="ACG8ZM_000592"
     CDS             24460..25095
                     /locus_tag="ACG8ZM_000592"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0491580.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25125..26480
                     /locus_tag="ACG8ZM_000593"
     CDS             25125..26480
                     /locus_tag="ACG8ZM_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0491581.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26672..28066)
                     /locus_tag="ACG8ZM_000594"
     CDS             complement(26672..28066)
                     /locus_tag="ACG8ZM_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0491582.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28158..29129
                     /locus_tag="ACG8ZM_000595"
     CDS             28158..29129
                     /locus_tag="ACG8ZM_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0491583.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29126..29908
                     /locus_tag="ACG8ZM_000596"
     CDS             29126..29908
                     /locus_tag="ACG8ZM_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0491584.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30077..30448)
                     /locus_tag="ACG8ZM_000597"
     CDS             complement(30077..30448)
                     /locus_tag="ACG8ZM_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0491585.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30582..31025
                     /locus_tag="ACG8ZM_000598"
     CDS             30582..31025
                     /locus_tag="ACG8ZM_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0491586.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31068..31877)
                     /gene="yidA"
                     /locus_tag="ACG8ZM_000599"
     CDS             complement(31068..31877)
                     /gene="yidA"
                     /locus_tag="ACG8ZM_000599"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0491587.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(31896..33263)
                     /locus_tag="ACG8ZM_000600"
     CDS             complement(31896..33263)
                     /locus_tag="ACG8ZM_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0491588.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33247..34077)
                     /locus_tag="ACG8ZM_000601"
     CDS             complement(33247..34077)
                     /locus_tag="ACG8ZM_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0491589.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34240..34677
                     /locus_tag="ACG8ZM_000602"
     CDS             34240..34677
                     /locus_tag="ACG8ZM_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0491590.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            34753..35373
                     /gene="rpoE"
                     /locus_tag="ACG8ZM_000603"
     CDS             34753..35373
                     /gene="rpoE"
                     /locus_tag="ACG8ZM_000603"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0491591.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            35562..36068
                     /locus_tag="ACG8ZM_000604"
     CDS             35562..36068
                     /locus_tag="ACG8ZM_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0491592.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            36085..36984
                     /locus_tag="ACG8ZM_000605"
     CDS             36085..36984
                     /locus_tag="ACG8ZM_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0491593.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            37071..37955
                     /locus_tag="ACG8ZM_000606"
     CDS             37071..37955
                     /locus_tag="ACG8ZM_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0491594.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(38147..38218)
                     /locus_tag="ACG8ZM_000607"
     tRNA            complement(38147..38218)
                     /locus_tag="ACG8ZM_000607"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38183..38185),aa:Arg,seq:ccg)
     gene            38278..39612
                     /gene="rpoN"
                     /locus_tag="ACG8ZM_000608"
     CDS             38278..39612
                     /gene="rpoN"
                     /locus_tag="ACG8ZM_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0491595.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            39761..40852
                     /locus_tag="ACG8ZM_000609"
     CDS             39761..40852
                     /locus_tag="ACG8ZM_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0491596.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            40893..41894
                     /gene="gap"
                     /locus_tag="ACG8ZM_000610"
     CDS             40893..41894
                     /gene="gap"
                     /locus_tag="ACG8ZM_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0491597.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            42004..43194
                     /gene="pgk"
                     /locus_tag="ACG8ZM_000611"
     CDS             42004..43194
                     /gene="pgk"
                     /locus_tag="ACG8ZM_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0491598.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            43298..44053
                     /gene="tpiA"
                     /locus_tag="ACG8ZM_000612"
     CDS             43298..44053
                     /gene="tpiA"
                     /locus_tag="ACG8ZM_000612"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0491599.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            44169..45467
                     /gene="eno"
                     /locus_tag="ACG8ZM_000613"
     CDS             44169..45467
                     /gene="eno"
                     /locus_tag="ACG8ZM_000613"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0491600.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            45866..47005
                     /locus_tag="ACG8ZM_000614"
     CDS             45866..47005
                     /locus_tag="ACG8ZM_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0491601.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        15630 a         8147 c        10038 g        13235 t
ORIGIN      
        1 tattcgccaa aaatttatca agtgataaaa ttgttgtata aaatgattat ttcaggagga
       61 ggagttaaat gtttttagca ttaaatgaaa ttatgcattc aaagttacgt tatgcgttag
      121 ttgcaggggt gatgtttttg atcgcctacc tggtattttt tttgactggg ttagcttatg
      181 gcttagctca agataatcga acagcagtcg acaagtggga ggcagattcg attgtgcttt
      241 cgaaagatgc aaattcaaat ctagggatgt ccatgattac taagaagata gcagaagaag
      301 tcgaaggtgg gaaagtggcg tatcttgccc aaacacctgg agtcgtgact agtaaagaca
      361 gtacagaaga aggaaaaatc aatgttagct tcttcggtat cgataaaaat caattcatca
      421 tgcccaatct tgttgaagga aaagcttttg ataacgatga tgaggcagta ggcgatatta
      481 gtttgaaaga agaatatggt ctagccatag gagatacagt taagttatct ggtagtgaca
      541 agacgtttaa actaactggc tttacggatc atgccaaatt caatgtctca ccagttctct
      601 atacaaccat caatgcttac caagaaatcc gcttcgaaaa agaagataca agtgaaaatg
      661 cacggattaa cgcaatagtt gtcagaggta aaatcagtaa tttacctgaa gatttagaac
      721 agatcaaaat ttctaaattc atcaatgaat tacctggata taatgcgcaa gtattgactt
      781 ttggatttat gatcggattt ttgatcgtta ttgcggctat tgtgatcggg attttcattt
      841 atgtactgac gatgcaaaag attaatatct ttggtgtaat gaaagcacaa gggatcacag
      901 gaggatttat tgcgcgttct gtagtggcac aaacattcat tttatcattt gttggtattc
      961 ttcttggtct attaggaaca gtgggaacat cccttgttct acctgatgcg gttccattcc
     1021 aaagcaattg gctgtttttt ggcgtcatta gcttgttgat gttagtagta gctgtgttag
     1081 gcgcactatt ttctgttcgg acaatcgtaa aaatcgatcc gttgaaggca attggataag
     1141 aaggaggaag aacatgaaag caatagaatt taaagaagta gaaaaaagtt tccaagatgg
     1201 cgaccaagta gttaaagcat tgaaagaaac aaccatgagt atcgataaag gggaatttgt
     1261 tgcaatcatc ggtccttctg gttcaggtaa aagtactttt ttgactttgg caggcggatt
     1321 gcagacgcct agcagtggct ctgtatttat caatgatcaa gaattcagta atcaaaaaga
     1381 aagcgaacgc gttaaactac gttttaaaga aattggcttt attttgcaag cttccaatct
     1441 ggttcctttt ctgacagtaa aaaagcagtt attattagta gataagatta aacatgaaca
     1501 tcgtcaaaaa gaagcaaacg aattatttga gcagcttggc gtggataagt tgatcaacaa
     1561 gtatccagaa gaactatctg gtggagaacg gcaaagggtc gcaatcgctc gtgcattgta
     1621 caatgatccg tccattattc tagcagatga accaactgct agtttggatt cagaaaaggc
     1681 acgagaagta gtcgatatct tggcaaaaga atcaaaagac aaaaataaag caacgatcat
     1741 ggttactcac gatacacggc tgattgacaa atgcgataga atatttatga tggaagatgg
     1801 tgtattgaaa gaaaaagaat aaccaaaaag caggtggaaa aatggtccat cgatacaaca
     1861 aagatacaca gcaaaaaatt ttgaaagaag cgactgactt gtttatgtcc aagggatatc
     1921 tcggcacttc aacaagagaa atcgcacaaa aagttgggat cacccagccc aatctctatc
     1981 attatttcgg tgataaagaa aaactatata cggcagtttt agaaaaccac ttaaaagatg
     2041 ttggaaaagc tttgagagaa atcgttcaaa caagcgaaac gggatttcaa gtaaccttga
     2101 caaaaatggc gcagtttttg attgagacac atctggtcga tttgtttctg atgcttcacg
     2161 atcttgaaag caatctttca aaggaaacaa gagatcatct tttctttcta tggaagaaaa
     2221 attatcgtga accttttgaa gaaatctttt cagcaaacca atctgtgcta agaaatggga
     2281 tttcaaaaga aatcgcagca aggcattttt tcttagtgtt agcaccttac atcactcggt
     2341 cgaaaagacc tgtggaagaa ccgctaaatg tggcgcaatt gatcgattta tatttgaatg
     2401 gtgtattagg agaagcgtaa agctgttata tagggagaaa atagttaggt gaagaagcaa
     2461 gatatgacga agatcgttat atcttgcttc tttatatagg tagtttttca taaagacaaa
     2521 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
     2581 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
     2641 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
     2701 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
     2761 ttacttaagc tgattttaag ttttggccct tagtctggat ctacgaggtg agagtcaaga
     2821 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
     2881 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
     2941 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
     3001 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
     3061 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
     3121 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
     3181 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
     3241 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
     3301 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
     3361 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
     3421 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
     3481 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
     3541 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
     3601 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
     3661 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
     3721 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
     3781 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt tattttttta gtagcttcaa
     3841 agctttttct gtatttgcaa agtgaagttt ggcatatttt tcaagcatag gcatgccgcc
     3901 tttctccaat agttttgccg ctacttggtc agatttcgtt ccagctcctg atggaagggt
     3961 gattccagct tcagctgatg ctttgtgtaa ctcttccaaa aagctggcac gactgatgat
     4021 agaagcagca gcaacagcta aatgatattg ttctccttta gtaacgaagt acagtttttc
     4081 ttgtaattga tttttttcta aacgcgcata ttttttaaaa tttgcttccg gcgtaaactg
     4141 atcgatcaaa atcgcttctg gtttttctgg ctcgattttt tttagcagca aatggatcgc
     4201 ttgattgtgc aaagcgactt tcatccgaac ggcattgtat tttggttgga tctcattgta
     4261 tttttctgga gataagacaa gcagtttata ggggatggtt ttttttaaga tgtcagcaag
     4321 ctggatgatt tggctatctt ttaactcttt tgaatctcta acaccaagtt tacgtaattc
     4381 tgcaagctga tcacgtttca cataagcagc acatactgtt agcggaccga aatagctgcc
     4441 atttccgacc tcatcactac ctaaaacgga taattgactg atattttttg gcaaagaaga
     4501 ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc ccccatttat cagcttctat
     4561 tgctgcattc gtaccttgaa acatcacttt tccggatgta taagcggtaa tggtcgtccc
     4621 gtcttttttt gcagcaaagc tagtataagg aatctgttta tttaacaaat aagagcgata
     4681 gtatgctttc atttgactaa gttggtcgtt tgttgctttg ataatgacgt tgctcataaa
     4741 attctcctcg tttttataca taaaactata ccgcaactca cgacttttcg ttttctaaga
     4801 gattgcgaga catagcattt ttttaaagtt tatcatataa tagttaagta tgtgaaaaat
     4861 atttgtggag aacggtgaaa gaaccaactt catggtacac taaataaaac tttcatcaaa
     4921 taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct acaaagcggt gattgccaac
     4981 caaacatata ctattatcgg acgggaaacc aagcatcata tggatatcgt aacgaagctg
     5041 atcaatgaac agttagcaga attgaaacaa ttatcaccac aaatggataa tgaacaagca
     5101 gcaatcttga tggctgtcaa tgcgctttcg gatcaattga agaaacaaga aagaatcttg
     5161 gagctggaag aagaaacagc agaattaaag aaaaaaatga tcaaatttac agagcttgaa
     5221 aatcgtgtga aacgcattga agcaatcgag aacgaagctc gggaagtatt gaaagaaaat
     5281 ggtcaagcag atcataccat taaaaatcat gtggaagcac aacagatttt aaacgagaaa
     5341 cgaaaaggac aaattaaaca aaaatcttct aattaagatc cttttatcaa aataagaaaa
     5401 atttaaaaaa agttagaaga gaaaggaacg tagcatgctg agtttgctga ttttattttt
     5461 attgctaatt gcgttttttt ctggagcacg tcgcgggttt gctttgcaaa tcatattcgc
     5521 gattggatat gtattgtcat ttattgctgc ccagcatttt tacaaaccat tagcaagtca
     5581 tttggaatta tacattccat acccagctgt cacgccaaca tcaaagttag cattttttga
     5641 tcaggtattt gcctttcacc tagatgaagc tttttatgca ggaactgctt ttctgattat
     5701 tttgctaatt ggctggctgc ttacccgatt tgtgggggtt tttgtccatg gattgacgta
     5761 tgtgccgatc ttgagacagg tagactggat tgcaggaggc atcctgtcac taatcatggc
     5821 ttacgttact atatttttga ttttgcagtt acttgccttt gttccattag atgtggtcca
     5881 aaatcaattc ggcgagcata gtttcgctcg ctttatcgtt gagagaacgc catttcttac
     5941 caacaagatg tatgagcttt gggtaacgaa tattttgaat taaggaaaat agtgctggaa
     6001 aatatttcgt tttttgtaca gagtagatta tttaagaagg ctgcgtagcg tatgtccggc
     6061 acagctcttt tttcatagtg aaagataagg tgataaaatg aataaccgaa tccttgaaac
     6121 attggaattt gaaaaagtca aacagatggt cagacaattt gtagtgactg cacaaggaaa
     6181 agaagaactg gcagaattgg taccagtgag cgaggaacaa acgatcacaa actggcttca
     6241 agaaactgag gatggattga aagtgcaacg tctgcgaggc gggatcccta tacctaaact
     6301 tgaaaatatc cgtccccata tgaaacggat tgagatcgga gcagacttga atggagtgga
     6361 gcttgcacaa gtttccagag tattgtctac gacaagcgaa ttgaatcgtt tcattgatga
     6421 tctttatgac agtgaaatcg aatttgcacg tttgtatatg tgggcagatc agttagtcac
     6481 gatccctgtt ttgagccgcc gtttaaagga agcaatcgat gaagacggac gcgtgactga
     6541 tgacgcttct cctgagttaa agagcataag acagaacatt cggcgaagtg aacaagctgt
     6601 tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa gtgatgcgat
     6661 catcaccatg cgaaatgacc gctacgttat cccagtcaaa caagaatacc gcggtgtatt
     6721 tggcggtgta gtccacgatc aaagtgcttc aggacagact ttatttattg aaccaaaaca
     6781 agtggtggat ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa atgagattca
     6841 acgtatttta agtgaacttt ctgctgaact tgttccccat cgtcaagaaa tcatccacaa
     6901 tgcttatgtg attggtaaaa tggatctgat gaatgcaaaa gcacgatttg gtaaagaagt
     6961 aaaagctatc gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac aagcaagaca
     7021 tccactgatc gatcaggaaa aagtagtttc taatgacatc acaatcggaa aagactatca
     7081 agccatcgtc attacaggac ctaacacagg tgggaaaacg atcacgttga agactttagg
     7141 tctgttacaa ctgatggggc aagcaggatt accgattcct gcaggtgaag aaagtcaaat
     7201 cggtattttt gaggaagtat ttgcggatat aggagacgaa cagtcgattg agcaaagtct
     7261 atcgaccttt tcttctcata tgacgaacac tgtagatatt ttatcaaaag tcaatgaaaa
     7321 aagtctggta ttatttgacg agctcggtgc aggaacagat ccgcaagaag gtgctgctct
     7381 agcaatcgca atcttagatg atcttggtaa aaagtctgct tatgtgatgg caacaacgca
     7441 ttatcctgaa ttgaaagttt atggatataa tcgggcaaat acgattaatg ccagcatgga
     7501 gtttgatgtg gatacattaa gtccaacgta tcgtttgctg attggcgttc ctggacgaag
     7561 caatgcgttt gaaatctcta gccgattagg tcttgataca gaagttattg atgaagcgaa
     7621 gcagttgatg aatgatgaga gccaagattt aaacgaaatg atcacagatt tagagaatcg
     7681 ccggaaaatg gcagagaccg aatatctgga aatgcgtcat tttgtttctg aggcacaaga
     7741 acttcacgat gatttgaaag aagcttacag ctatttcttt gaagaacgtg aaaaagaaat
     7801 ggaaaaagca aagaaaaaag caaatgaagt cgtgtcagag gcggaagaaa aagcagaaaa
     7861 aatcattgct gatattcgta aaatgcaaca gcagattggg caaggaaatg tgaaagagca
     7921 tcagttgatc gatgcaaaga cccaattagc aaatctacat caagaagaaa ctttgaagaa
     7981 aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag acgaagtgtt
     8041 ggttacgact tacggccaaa gagggacgct tttacgtaaa aacgggaatc agtggcaagt
     8101 agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg ttgctcctca
     8161 aaaagagccg acacaacgag ttatccatgc tgtacgatct gaatctagta gtcacgtgcc
     8221 gaatcaattg gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg tagatcaata
     8281 tttggattct gccattctag ctggttatcc tcaagtgact attgtccacg gaaaaggaac
     8341 aggcgcactt cgcaagggaa ttactgatta tcttaaaaac catcggagcg tcaaaagctt
     8401 tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat ttaagtaaag
     8461 cttactaaag gtaagcagat ggttcgctat ccaagtatag aaatgctata ctagtcatag
     8521 attttaaaaa tggaggtctt tgatatgtca caagtaatta cagataagac atttaatgaa
     8581 gaaacagata aaggacttgt attgattgat ttttgggcaa cttggtgtgg tccatgtcgt
     8641 atgcaagcac cgattttgga tcagttggaa caagaatatg atgaagaaga attcagaatt
     8701 gctaaaatgg atgtggatga aaatccagaa actcctcaac aattcggtat catgagcatt
     8761 ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg tgttcattca
     8821 aaagaacaac tacgtcaaat gatcgatcaa tatttataaa aacttacagc aaaaataagc
     8881 tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt taaggttatt
     8941 caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt ttgacagaca
     9001 tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac gaatcaaaaa
     9061 caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata aaaatggaac
     9121 gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat attttactgg
     9181 tagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg aatacatcgt
     9241 tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga aaaatgatcc
     9301 taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga taacgaatga
     9361 aaaatatccc cgtttggtga ttactcgtaa ggtactaaaa gacaaagcac tgtattttgg
     9421 accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc gattatttcc
     9481 tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc atttgggaca
     9541 atgtctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa tcgttgatga
     9601 agtgaaaaac tttttaaacg gaggacacga aaaaatcgag tcagagatcc atcagaaaat
     9661 ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag accagatccg
     9721 tgccattgag acaatcatga ctcgccaaaa aatgacaaat actgatttac tggatcgaga
     9781 tgtatttggt tatgcgattg ataaaggatg gatgtgtgtc caagtgtttt tcgtaagaca
     9841 aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag cagaagaaga
     9901 tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc ctaaagaagt
     9961 cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta cgaaagtgct
    10021 gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgagtaaga atgcctcagt
    10081 tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga tcggagcagt
    10141 tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt cttttgataa
    10201 ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg atggaaaacc
    10261 ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg atgattatgc
    10321 atcaatgaga gaagtcatct atcgcagata ttcgcgagtg attcgcgaag ggttgccttt
    10381 acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa aagatgtctt
    10441 agaaaatcaa ttaggaatcg atattcctat tgctggcatg gcaaaaaacg ataaacataa
    10501 aacaaatgag ttgctattcg gtacagattt ggctgtagta ccgcttgagc gaaattcacc
    10561 agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa tcactttcca
    10621 tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg aagggttagg
    10681 accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata tcacagcagc
    10741 cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa acgtatttga
    10801 taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg tcctatcatt
    10861 ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg cttacgtctg
    10921 gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag aagtcaagga
    10981 tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc aaatccccga
    11041 tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga cttttcgttt
    11101 gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc atcatcttgg
    11161 ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt atccgaaagt
    11221 aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa gaaaagagat
    11281 tgaaccaaaa atcgactttt ggatggaaaa atttcaggta aaagggaaaa agacggataa
    11341 agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca cgttaatcca
    11401 tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg taaatgctga
    11461 attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca ttttttctag
    11521 ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc gaaacggaaa
    11581 aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta cgaagttatt
    11641 cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga aaaaagtgat
    11701 ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg ggaaagaaat
    11761 ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac cgccaacttt
    11821 ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg attattacca
    11881 aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata ttagtccctt
    11941 ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat agtgatgtca
    12001 ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc caagtcaaaa
    12061 cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa gagctttcgg
    12121 aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata aatgggatgc
    12181 tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa caactgactg
    12241 gcttgcaatc gatgcttcga gcgagtcaat taggcatttc accagaagaa gtagctagct
    12301 tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa ggaaaaatga
    12361 cagtcggaga agataactcg acagtccaat acgtcgtaag ttatgtagca acgattgtct
    12421 tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct tctgaaaaag
    12481 gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat ttttacggaa
    12541 aattaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt attatttttg
    12601 gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta gacggtatct
    12661 cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg atctttggca
    12721 ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa gcagaagata
    12781 cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatacatg cttggattga
    12841 tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat attccattct
    12901 tatcttcata tattatgccg gttcgcttag caaatgagac agtcggtatg ggtggagcat
    12961 tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg tctgcaaaca
    13021 tgtacaaatc gaatgtactc gtttatagcg aaggcggatt atggagttca ttgaaacaat
    13081 cgatttcaat tatgaggaat gaacgaaaaa aagcataaaa caatcctaaa atacagaagg
    13141 aagctggaca tctattgttc ccagcttctt tttgtataga cctattttat ttattttact
    13201 atattggata tatattcatt gaaaatagtg tataacaact ttataatatt gagtatacca
    13261 atttttggag gcgttttaat ggaccgttat ttagatttac tttcagaaaa attcccctca
    13321 acggaggatg tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct tccaaaagga
    13381 acagagttat ttttgagtga tatacatggc gaatttcctg cttttgatca tattctgcgt
    13441 atcggttcag gaaatcttaa agaaaagatc aaagaccttt tttctgaaca aatgacggaa
    13501 acggatatca gccaattcac tatttttacg gcttatccag aatatgcatt aacgactgat
    13561 tggtacaaag aacaagataa atcgcagctg atccatcatt tgatcgatct tttacggttt
    13621 acgacagtca aatatacccg gtcaaaagtt cgaaaaggat tgccgaaaga atatagctat
    13681 atcattgagg aactcttgta tatcgatgac cgtatcaatg gaaaaaaaga ctatgtaaaa
    13741 aaaatcattg aacaacttgt tttgatgaag gaagaagaaa aatttttaac gaaacttgca
    13801 cagacgatcc aaaaatctgt catcgatcat ttgcatatcg ttggagatat ttttgaccga
    13861 ggaactcaag cagccaaagt gatggatcga atcatggatt tttcttctgt agatatccaa
    13921 tggggaaatc atgatatttt gtggattggt gcgtattgcg gctctgaggc atgtttactg
    13981 acacttttac ggattgcagc acgctataat tatttatttg aattagagaa agaatatggg
    14041 ttgaatttac gtccattgtt ttcttttgct catcaaactt atcagaagaa tccagtattt
    14101 gcgcctaaaa acagtaaaaa tctatccgaa agagagcaag aagaattaga aaaaatccat
    14161 caggcacttt ctatccttca attcaaatca gaacatcagt tattggatcg tcgacctgaa
    14221 tttaaaatgg acgatcgtaa attgctggaa aaaatcgact atgaagcatc aacgattgat
    14281 ttagaaggtc agctctatga gttgaaagga acttgttttc aaacgatcca gccagatgat
    14341 ccaaaaaaat tcttggaaga ggaacaaaaa gtgattgata gtttgatgta ttcattccag
    14401 cactctttga gaatgcaaaa acatatgacg tttttgatcg aaaaaggggg aatgtatctc
    14461 acgaacaatc agcatgtgct gtttcatggc tgtatacctg tagacgagaa gggacagctc
    14521 ttagcatttg aattagacca atcttatcgc ggaaaagaac tgctgggctt ttttgaaaaa
    14581 cagataactg aaagcgcaaa agatctgact gcaaaagaag atttagcaac cgatttgatt
    14641 tggtatgcgt ggagtgggcc attttcgcct ttattcggca agagtaaaat ggcgactttt
    14701 gaacgttatt ttctcacaga gcctcatacg cacgtcgaga aaagcaatcc ttattatcac
    14761 ttgcgagatg aagagtggtt cagtgaaaaa attttagcag aattcgacac aaaagaagaa
    14821 gggtcagcaa tcattaatgg gcacacgcca gtaaaagtaa aaaaagggga gtcaccgatc
    14881 aaagcaaatg gaaaagtatt tgtcattgac ggaggtttat ccaaagctta tcaaaaaacg
    14941 acaggaattg ccggttattc actattatgc aattcttatg gtttccaaat cgtgacacat
    15001 tatccgtttt tgcctattga acaacaattt gcaaaaaaat ccgaccaaac aaatgtgaaa
    15061 aaagtaatcg aacagcaact accacgcaaa ctcaatcgtg atacaacaaa aggtatggaa
    15121 ctgcagcagc agattaccca gctcaaaagg ctgttggact atcgaaagga cgattgagaa
    15181 gaattccagt gaacattgtt ccatagactg gagactgaga caaaagtcgt gtcgcagtct
    15241 ctttttccgg ataaacggtg ttcttctgcc aactcctcgg tcacaagtca cactattcaa
    15301 caaacatgaa aagctgtttt ttgaaattgc tccttgtgac tcagagttga acggcaggct
    15361 cacgacctct tggtataaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta
    15421 tgcaaaaatg tgaaaagcca ttttcacaaa atttttctta atactcgaaa ctaaacagct
    15481 ttttcacaac cccctcagaa agtctattaa aattcattca caaagaaatc gaataagcgc
    15541 tgttcagatg gatgtacacg gtgggtaagt tccggatgcc attgaacacc gaggatttta
    15601 tgcatatcat ctttgctttg aaccgcttca atgagtccat cgttagaaaa agcgatggct
    15661 tttaataaag gtgaaagatc tttgactgcc tgatgatggt aagaattcac aaaatatgga
    15721 tcggggagaa ggttgcttaa caaactgtct gaaacaatct tgacttcgtg agtcgtaaat
    15781 tgcggggcag tgggctgttg ttcatgtttt actgaccact ttggatataa tgaaagatct
    15841 tgataaagtg taccttccaa aacgacattc aatagttgga gtccccggca gactgcaaaa
    15901 attggttttc tttgtttcaa tgcttccaag atcagtgcct gttcaaattg gtctcgttga
    15961 atatttgttt ccgttagctt tggatgagga tcttccatgt aaagttgtgg actgacatct
    16021 tgaccacctg caagaataag tttgtcgatt tgtccaatca gttgaggagc tgaatctgga
    16081 gacatgactg gcagaataag aggcaatcct tgagcatcta aaactgcatc cacgaatcct
    16141 tgcggtgtat agctcacttg atttccttgg aaagtatctg ttgctcgaat cagttgattt
    16201 ccggcaatgc cgatgattgg tttcatttgt attcccccta aagtagactt attgtaacaa
    16261 ggatttaaaa aaaattaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg
    16321 ttactatctg taaagagaaa tttcgtgtga aattcatgac gatagttgaa aatattgctt
    16381 aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct
    16441 agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct
    16501 ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg
    16561 aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaaa
    16621 agcgtgctca aacactgcgg cagcagctga agatctatgc aacagaaaaa gagcaggaat
    16681 tggaaacgat ccaaacaaac tatcgacaac aactaaaaca gttggtggaa gaaaaagaac
    16741 aatttgaaat acaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg
    16801 tgcaagcaac tttgaaagat caatatcaaa agcatcagag tcaagcgatc ggtgcgatca
    16861 ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca
    16921 gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa cgttgaaatc
    16981 cgggatctgt atcaaaatag aacaaacaac cagtgggtgg agaagtattt tccagatacc
    17041 acgattattg ataaagaagc aaaattaagc gctttaggta atcggttgaa agacatccaa
    17101 gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa
    17161 cgtcaagaac tttccttaca agatttggaa gccaattatt ccgaagaaaa tttcataaat
    17221 aaattgaaag aagtccttga tttaaaagaa aagtgggaac aactaactga agaacgtgat
    17281 cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt
    17341 atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa
    17401 agtttcaagg aagaactgag taagcttgat gacgtttata tggaagaaat agattatacg
    17461 ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgt tatagaagaa
    17521 attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa
    17581 aaactgcaag agaccaagca gcaattatcc attgttcacg agcagcaaaa gaagataacg
    17641 acagcgttag gtcagtgcag tggctatatc caagaatttg aatgggtaga agaagtcaca
    17701 cttgccgttg cagaacgaga aaaaataaaa gaacggttta tccatgcgac ttatctgatc
    17761 gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgctgcatgt gttaaacgaa
    17821 accgtatcag aagaagatat atacgtcagt ttcgaaacac caaactcaga agagattcag
    17881 caggaagtcc ctactaaatt gaaaaaccat ccgcttgtcg aaccttttga attgctgact
    17941 gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa caccttggat gacaccattt
    18001 tatcttgtct tcttcgggat gatggtcgca gatgttggtt atggtttgct gatgctcatt
    18061 gggacaatac tagctcaaaa actacttgtt ctaccacgcg ggatgaaacg tttcgtaaaa
    18121 ttctttgaga ttctctcgat accatcgatc atctggggat tgatctacag ttcatttttc
    18181 ggacagtcat taccaaaaga actgtttggc atccgcttgc ctttcccaat cctatctacg
    18241 acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat acagattttg
    18301 gttggattat ttgtggcagc aaaggaaaat atcaggagaa aagattactt gagtgctgtc
    18361 agtgatggat ttgcctggca agggatccta attgggatcg ttattgcgtt ggttggtgcc
    18421 atgttgataa aaaatacagc atttgtttac cttggcgctg ggctatcgat cattgctgcc
    18481 ttatgtatct tgatcgttcc gattatccaa acgccgtcta aagtcaaagg tgctgccaaa
    18541 ggtgcgtata atctttacgg actgaccggc tatatcggtg atttagtcag ttatacacgt
    18601 ttgatggcat tgggtatttc aggcggaagt attggagcgg cattcaatat gctagttgct
    18661 ttcatgccgc cagctgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca
    18721 ctgaatatgt ttttgacact gctaagtgcc tatgtccatg gcgcacgatt gcagtatgtc
    18781 gaattttttg ggaagtttta tacaggaggc ggacgtgcct ttgatccgct gaaaacggcg
    18841 gaaaaatacg tcaacatcaa tcacaagaaa aaaaacaata aataattgga ggatttttat
    18901 ataatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcggt attagcgatg
    18961 gcaacagcga cgattttttc agggatcggt tcagctaaag gtgttggaat gacaggtgag
    19021 gcagcagcag cattgacaac aagtcaaccc gaaaaattcg ggcaggctct gattctgcaa
    19081 ttgcttccag gtacgcaagg tttgtatgga ttcgttattg cctttttgat ttttatcaac
    19141 ttaggaaatg acatgtcagt tgtacaagga ctgaactttc taggtgcttc tctgccgatt
    19201 gcttttacag gcttgttttc aggtattgcc caaggaaaag tcgcagcggc aggtattcaa
    19261 attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa
    19321 acatacgcga ttttaggttt cgttatttcc ttcctattag tgttgaatgc ctaagcataa
    19381 aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaac gaagcagcag
    19441 agcaggaaag agcggcgtta gaacaagaag agcgaatgaa gatcgatcaa aactttgaac
    19501 aaaaacgtac gcaagttgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac
    19561 tcgaaaaaaa ataccgtcag ctaaggaatc gccagcaagt agaagtacga caagaaaatt
    19621 tgaatgcgaa gcaggaattt ttaagacgat tgtttgcaga tgcggtaact gaaatggaaa
    19681 attgggatga atctgaacaa atccagttta tcaaaaatgc attgtattca ttacctttga
    19741 caggaaaagt agcattcatt gctggagaaa aatcagcagc ctatctttct cagacgttgt
    19801 tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg
    19861 atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc
    19921 ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag
    19981 gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga
    20041 acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg
    20101 tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt
    20161 cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc
    20221 ggagcctgaa atcgtttgga tctatacgat gcgttatact ttccataatc tgaaggtttt
    20281 gacaaaagct gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta
    20341 ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac ttcctgaaag
    20401 cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg
    20461 aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct
    20521 aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac
    20581 gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag
    20641 ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta
    20701 cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg
    20761 aaatattgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca
    20821 agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga
    20881 ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaatcaga
    20941 aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca
    21001 aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc
    21061 aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca
    21121 cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac
    21181 ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc
    21241 aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa
    21301 ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt
    21361 ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta
    21421 tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc
    21481 caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca
    21541 tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa
    21601 gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt
    21661 tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag
    21721 aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca
    21781 aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa
    21841 atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca
    21901 aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct
    21961 tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag
    22021 tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg
    22081 gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta
    22141 atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag
    22201 tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca
    22261 tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg
    22321 agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt
    22381 cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc
    22441 gcgaaggaag tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac
    22501 cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag
    22561 cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag
    22621 aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg
    22681 gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg
    22741 attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt
    22801 atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca
    22861 aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg
    22921 cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca
    22981 aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa
    23041 cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc
    23101 ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa
    23161 ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa
    23221 gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct
    23281 tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga
    23341 gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat
    23401 ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc
    23461 caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca
    23521 gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca
    23581 acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt
    23641 gaagaagctg aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc
    23701 atgtttatga atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct
    23761 ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca
    23821 gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca
    23881 ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca
    23941 ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa
    24001 gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc
    24061 ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt
    24121 tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg
    24181 atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt
    24241 ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt
    24301 gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat
    24361 ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac
    24421 atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat
    24481 ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat
    24541 aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag
    24601 aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta
    24661 ttggcaaatg cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa
    24721 aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat
    24781 tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt
    24841 gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg
    24901 acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga
    24961 gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt
    25021 atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg
    25081 ggtaccactg actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca
    25141 aacaaataaa gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga
    25201 ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg catgggacaa
    25261 attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat
    25321 tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga
    25381 tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag
    25441 tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg
    25501 tgatgaagct catgctttac atcctaaaat tggcctttgt gatccaattg attggtacgg
    25561 ttgctttatc ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt
    25621 tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag
    25681 ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg
    25741 ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga
    25801 ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt
    25861 tctttttcac agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg
    25921 caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc
    25981 tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat
    26041 ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc
    26101 gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg
    26161 cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca
    26221 tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg
    26281 aggtcttctc tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg
    26341 caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg
    26401 tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag
    26461 aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa
    26521 gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt
    26581 tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca
    26641 ataagatttg attcctttat ttatcaatag gttataattc atattttttt attttttctt
    26701 ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa
    26761 cgaatgcatc tggttgcgta cgtgtagtaa agcgtctgga atgataaagt cggataccgt
    26821 tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc
    26881 gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat
    26941 tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga
    27001 ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg
    27061 cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag
    27121 aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga
    27181 aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa
    27241 taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt ttgtgagaga
    27301 tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt
    27361 ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa
    27421 cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg
    27481 ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa
    27541 caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat
    27601 ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa
    27661 caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt
    27721 caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg
    27781 tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca
    27841 aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat
    27901 aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa
    27961 tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt
    28021 ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt
    28081 aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt taatcttttg
    28141 tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt
    28201 ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca
    28261 gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga
    28321 tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc
    28381 cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc
    28441 aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg
    28501 ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa
    28561 ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca
    28621 ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta
    28681 aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc
    28741 cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg
    28801 taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa
    28861 gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag
    28921 aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg
    28981 gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga
    29041 cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata
    29101 acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc
    29161 agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga
    29221 aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa
    29281 gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg
    29341 tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac
    29401 gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta
    29461 catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc
    29521 gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga
    29581 tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg
    29641 aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt
    29701 tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc
    29761 ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg
    29821 cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt
    29881 gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg
    29941 gagagtggca accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt
    30001 aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta
    30061 aaatgcccca tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga
    30121 caaagtagta agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccag
    30181 cagctttatg ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat
    30241 agccgtctcc tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt
    30301 catccccagg aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc
    30361 ggctttcttc aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca
    30421 ctcgaacaca tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc
    30481 ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt
    30541 aaaatggata gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg
    30601 gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa
    30661 gagatcagca gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg
    30721 aaattgaagg agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg
    30781 gaattgtggc agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt
    30841 atcgtgcaac ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta
    30901 taggagaacc aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg
    30961 tgaaattgaa acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa
    31021 actgaaagaa agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat
    31081 tttttgattg cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct
    31141 tctttgacaa gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg
    31201 gataagtcgt tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc
    31261 tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa
    31321 tagggcgcac ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt
    31381 gctgcatcta aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt
    31441 gtcatttctt caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc
    31501 gtataagcgc tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga
    31561 tgactgccga cacggcgtgc catcacttcg atctctagga aatcttcatg tgtcagtccg
    31621 tgacgggcga tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata
    31681 acgtaatcat tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag
    31741 ggacggccag tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct
    31801 tttacttctt ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg
    31861 actagtttga tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca
    31921 ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc
    31981 aaggacggat caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc
    32041 gctaatgcag caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc
    32101 aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga
    32161 tcgtagctag aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac
    32221 aaagtgagtt cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt
    32281 ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac
    32341 cattgagtaa agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta
    32401 aagcttccgt caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct
    32461 tcttgataca actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg
    32521 gaaacaggat ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc
    32581 actgcatgca tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt
    32641 aatattcggg taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc
    32701 aaatagtcca ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg
    32761 gggtaagttt tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta
    32821 agaatctgat acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga
    32881 ttcgtatgga agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac
    32941 agtgccgcac ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt
    33001 tcttttgaaa aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct
    33061 aatgaatgag taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat
    33121 tgtttaatgc gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga
    33181 tgctggacat ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt
    33241 tgatatttat aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct
    33301 ctggatttgg ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc
    33361 ctgtaaatca cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc
    33421 ttttacaaaa aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac
    33481 tgcaggatag ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat
    33541 gacttgcccg cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc
    33601 atttttgatc cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata
    33661 agttccagga caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc
    33721 gtatttagtc aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc
    33781 actttggggg aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc
    33841 acctgaattt ctgatcactg gagcgtaacc tgcatctttc agtgtttgga tcccgtcact
    33901 tagaaaagga gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa
    33961 atgtaaaaag atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa
    34021 aggggaaaag tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca
    34081 accggctcct ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt
    34141 gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact
    34201 aggaacaatg tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca
    34261 ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat
    34321 tttgatttga aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag
    34381 attcaggagg agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat
    34441 gtacagctga tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg
    34501 ttagaaacat cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa
    34561 ctgcatgtta gtttgaaaga ccgtcccaca gcagggaagg ttcgaatcga ttatgatctg
    34621 ttcatgggac cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata
    34681 taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag
    34741 gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg
    34801 atcgaagtag cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta
    34861 gctaaccaga tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca
    34921 caattttata ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg
    34981 ggcttgcgtt catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac
    35041 gaagaggatg aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat
    35101 gatgatgaag acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa
    35161 gaagatgacg atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat
    35221 aattctgact tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca
    35281 ggaaatatcg aagaagattt aagtatcata gacgaagacg atgatgatga agattatgaa
    35341 gaggaagaat tttctgactc tgaaaaagaa taaacattat tttcttacga gaggttgtga
    35401 cagagacgat cagcctatca gaataaggag gtgtgacacg atttttgtca caccttcttt
    35461 tttgtatata aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc
    35521 accttgtacg tcatattaag tgaggcagaa aggagtggag aatgattttt actggatata
    35581 agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa tggactcatc gaaactgatt
    35641 atcaaaaaat gttccgtatc acgatgaatt acgtacatga taaagaagag gccttagatg
    35701 tgatgcaaga tagttttcat aaagccctga actattttga aaaagaaaaa gagatcgagc
    35761 attttagtgc atggttttat cggatattga tccgcacggc attagatagt tggcgcagac
    35821 aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat cccccaacag ttacctttag
    35881 aaacttccgt tgcagagctc catatgattt tagataaaat agacagtccg gaaaaagaga
    35941 ttctgatcct ctactttttt gaaggatttc gtttgaaaga aattgcgact atcttggata
    36001 tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc
    36061 tagaatgaag gagagaagag tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag
    36121 aaaacttata aagaacaagc aattcccaaa gggcttaaag aggagatccg ccagcgtttt
    36181 attatcgaag agcaatcatt tataaaagca aagcggagaa agagacgtct ccaagggttc
    36241 ggagtatctt tggctatcgt aatgattaca acaagtagcc ttctttttaa tagtcaagtt
    36301 cgaagctttg cagaagattt acccatctta ggatcagtca ttgaattgat cttaggcgaa
    36361 cgctttactg atcgatctga aaaaatcgat attcaggtac cgaagatcaa tacacaaaat
    36421 gagaaagaaa ataaaacaat tcatggatta aatcaaaaat actttcgcga aggacaagca
    36481 gattttgaaa aagctgaaaa agaatatgga aattttgaaa cggatcatta ccaagttttg
    36541 ggagattatc agaaaatcgt agacgataac cggtttctcg tgattgaaag gcagatcacc
    36601 caaacagcag cagatagcca tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt
    36661 gtccagctct cacttccact tttattcaaa gatgacacct atctatctgt tttgacaaaa
    36721 gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc catctaagta ctattggact
    36781 gagcaagata ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc tacacgaagt
    36841 ttttatttga acaaagagca tcaattagtt ttgacttttt ctcaatacga aattgctcct
    36901 ggttatatgg gtacaccaga atttgtcatt cctaagtccg tgacaaaaac gattttggca
    36961 tcggaagatt atttggatca ttagacgaga agtagttgcg actagcagtt tcaattttca
    37021 agtaagtaca agtaaataat aagaagagaa aaagagaaag aagagatata gtgaaaagaa
    37081 attatttagt tatcagtatt attgctttat ccgtgctttt gtttttcgaa attacttcgg
    37141 tggcctcctt gatcaccgaa gtaaaaggac aaacacactc ttcggagcat tcgtcacaat
    37201 ctttcaaaca atcagaacaa tcaaccatta aaagccaatc aactgaaaca gctaacgtca
    37261 aagaagccat tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa tcaacacaag
    37321 acagtacaaa aacgagccga gatccggctg tcaaaagcgt cgcaatcagc tttgatgatg
    37381 gaccaggagc gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg
    37441 ccactttttt cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag
    37501 ctgaagcagg gcacgagatt ggcaatcaca catacgatca tcagaatctg gccactcttt
    37561 ctgcccagtc gatgacggag gaagtgacga aagcagacac tgagatcaaa aaagtcactg
    37621 gcaaaacccc cacttttgtt cgtccgccct atggttctgt cacgaatgta ggagcaacga
    37681 tcatccagcg accgatcatc gaatggtctg ttgattcaga agattggaag acaagaaatc
    37741 ctgatttgat tttgcagaag atccaagcaa cagtatacga tggggcaatt attttattcc
    37801 atgatatcta tcctgaaacg attcgtgccg ttcctcaagt gatcgattat ctgaaagagc
    37861 aagggtatcg catcacgaca gttggggatt tattagggca tccaacggct gtcgagaact
    37921 attatggcag aaacgatcat cggccagttc agtaagtgag acaagtatct ttaataaata
    37981 acacatttta ataagtagtt gaacacataa ctaccttact tgcaaaggct taacaatatc
    38041 tatacaggta caacaaaaaa ctgtccacca attttctttg gcggacagtt ttcacttaaa
    38101 aataaagaag caactattcg taaattatgc taaaatagca gtagaacgcg tcctgaggga
    38161 atcgaacccc cgtctcaaga accggaatct tacgtgatat ccactacact aaggacgcaa
    38221 gcactgggat aattgtaact aaacagataa aaaattacaa ggggattgga caaaaaaatg
    38281 aaatttgagc aaaatttttc acaaaaacag cagcagacac agaaactagc catgacgcaa
    38341 cagttgcaac agtccatcca agttttacaa tataacagtg aggagctgtt ggcattcgtt
    38401 gagaatcaag caatggaaaa tccattggtg gaagttgttg agccggaatg gcagccagat
    38461 tacataaaag cttcttcatc ttcttatgaa ggagaagaga caaattacct aaatcagatt
    38521 cctgatacaa aagggtcttt atttgattca ttgatcgaac aagtccattt gaattaccga
    38581 gatacttttt tgaggaaaat cgttctttat cttgtagaat acatcgattt gaacggattt
    38641 ttgacgatta gtttagaaga agccggaaaa gagattggag ctacgcctat ccaaatgctc
    38701 gatgcgttga ctctgattca gcaattagaa ccagctggag taggtgcacg tagtttgcaa
    38761 gaatgtctca tgcttcaaac agaacgagat gattatgcgc cggaattagc ttatatcgtg
    38821 ttggaagaat gttttgagga actggtggag cgaaaatgga aagaaatcgc acaacgtttt
    38881 tctgttgatc tacatgctgt tcagcagata ttcgactatt tacaaacgtt gagtccaagc
    38941 cctggccgga ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt ccgggtatta
    39001 gtggacgacg ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc aaatatacgc
    39061 tttcaagaga gttacttcaa acaaatgtca caacaagcag ataaagaaac tgaaaattat
    39121 ttaaaagaac ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca acgtggggat
    39181 acaattttgc gggttgctca agtcattgtt tcccggcaaa aagctttctt tatcgacaag
    39241 gaacgtccaa tcaagcctct tacgttaaag gaagttgcaa ctgagataga tgtgcatgaa
    39301 tccacggtca gtcgtgcagt caacgggaaa tatttggaaa cagacttcgg tgtatttgaa
    39361 ttaaaaaaat ttttcactac acgaattcct acgaatagta cagaacagac agaagacctt
    39421 tcagcagata cagcgaagaa aaagctgcag gaattagtcg atcaagaaga caaaaacaag
    39481 ccgttgtctg accagaaatt ggttgaattg ctgaaaaaag atgagatagc gatctcaaga
    39541 cgaacagtag caaaatatcg tgatttatta ggaatcccga gttcatcaaa aagaaaacgt
    39601 tatgataact aatcagtcaa aagactagaa aaagtatttc actttttcta gtctttttag
    39661 ttgaaaaacg agagcaaaac tgttagactt acagtcgtga ggttgagatt tccctttatt
    39721 gatgtgaaat tcgaatagtt aatttctttt tttatcgtat atgggtcgtt ttaagtctac
    39781 attggacgta aagtgtccaa ctaaggagat cgctatgctg gatgaaataa aaatgattga
    39841 atctgttgca ccagatatta tcgaagtgtt gcaagaacgc tataaaattt tacgaaatat
    39901 ctattggatg cagccgattg gacgtcgaag tttatcggaa agtatgggga tcacagaacg
    39961 agtattgcga acagaaacag atcttttgaa aaatctgaat ttgatcgaca cctcaaaaag
    40021 cggtatgacg ctgacaacta agggcgaaga agtttatcaa agccttgaaa acttcatgga
    40081 tcagctttta ggtacgcatc aaaccgaaca acagttggca gaatattttg gtaaccaacg
    40141 ctgtatcgtt gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg cattcggcga
    40201 agcgctgagc gaagccctag accgactact tccagaaggc gagaatatca tcgctgtgat
    40261 gggaggaact actatggctg tggtagccga acaattgtct aatctggaaa ataagaaacg
    40321 gcataacctg tttgtccctg caagaggcgg gattggtgaa gcgataactg tacaggcaaa
    40381 ttctgtcagt gcaagaatgg ctgctaaagc aaacggtaac caccgtgcac tttatgttcc
    40441 ggaacaatta agtctggcaa cttacaattc tttactcaat gagccatcta tccaagaagt
    40501 cttaaacttg attagtgaag cgaattgtgt tattcatagt attgggcgtg cattacacat
    40561 ggcagcacgt cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag caaatgctgt
    40621 tgcggaatct tttggctact ttttcaatga aaaaggtgaa gtcgtttata aggtccctag
    40681 aatcggcata gaattgcaag atttggaaaa agtcccgatc atcatggcaa tcgcgggcgg
    40741 aaaatcaaaa gctaaagcaa ttcgagctta tatgaaaaat gcaccaaaac aaacgtggct
    40801 catcactgat gaagctgctg caaatgagat tttaaaaggg gtaacccttt aaaataaata
    40861 tttttttgat tttcataagg aggaaatctt aaatgacagt taaagtaggt attaatggtt
    40921 ttggacgtat tggacgtcta gcattccgtc gtatccaaga tgtagatgga atcgaagtag
    40981 tagcaatcaa cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa tacgacacaa
    41041 ctcaaggacg tttcaacgga acagttgaag ttcatgaagg ttcattcaac gtaaacggaa
    41101 aagaagttaa agtattagct aaccgcaacc cagaagaatt accatggggc gaactaggcg
    41161 tagatatcgt tctagaatgt actggtttct tcacttctaa atcagcagct gaaaaacatt
    41221 taacagctgg tgctaaacgc gttgttatct ctgctcctgg cggaaacgat gttccaacaa
    41281 tcgtttataa cacaaaccac gaaacattaa ctggtaaaga aacagttatc tcaggtgctt
    41341 catgtactac taactgttta gctcctatgg ctaaaacatt gaacgacaaa tttggtgttg
    41401 ttgaaggatt aatgacaact atccacgctt acacaggtga ccaaatgact ctagacggac
    41461 ctcatcctaa aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc gttcctaact
    41521 caacaggtgc tgctaaagct atcggtttag taattcctga attgaacggt aaattagacg
    41581 gagctgctca acgtgttcct gtaccaacag gttcattaac agaattagta acagttcttg
    41641 aaaaagaagt aactgttgac gaaatcaatg cagcaatgaa agaagcttca aacgaatctt
    41701 acggatacaa cacagacgaa atcgtttctt cagatatcgt tggtatgact tacggttcat
    41761 tatttgatgc tacacaaact aaagtaatga cagttggcga caaacaatta gttaaaactg
    41821 ttgcttggta cgacaacgaa atgtcataca ctgcacaatt agtacgtact ttagaatact
    41881 tcgctaactt ataagataga caaacgtaca agtgtaaatt atagacatgt acatttaaaa
    41941 gcggggaagc aatcgcgctt cttccgcttt ttttgataat aagtttattc aggaggaaat
    42001 aaaatggcta agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt
    42061 gttgacttca acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca
    42121 gcattgccaa caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac
    42181 cttggacgtg taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa
    42241 cgtctaggcg aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa
    42301 ttagaagaag caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacga
    42361 tttgaagata ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg
    42421 gcttcattag gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct
    42481 tctaacgtag gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa
    42541 gaaatcaagt tcatcggtga agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt
    42601 ggtggcgcaa aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat
    42661 aaaatcttga tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc
    42721 ggtaactctc tagtagaagc tgataaagtg gaactagcaa aagaattaat cgaaaaagca
    42781 ggcgacaaac ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt
    42841 gaaacacaag taatcgaagg agacattcct gatggtttga tggctttaga catcggccca
    42901 gcatcagtaa aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga
    42961 cctatgggtg tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca
    43021 atcgctaact tagaaggcgc aacaacaatc atcggtggcg gagactcagc cgcagcagca
    43081 gaacaattag gatttgctga caaattcaca cacatctcaa caggtggcgg cgcaagcttg
    43141 gaattgcttg aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc
    43201 aagtttgctc aattctgacg agtaatcgaa caaaaggtga agggagtttt catgactccc
    43261 tagccatttt tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg
    43321 taactggaaa atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa
    43381 caaaatccct tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca
    43441 agaattaatg acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg
    43501 ggaaaactca ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt
    43561 tcattatgtg atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga
    43621 catcaataaa aaagcgaaag ctatctttgc aaatggtatg acaccaatcc tatgctgtgg
    43681 cgaatcttta gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac
    43741 agctggttta gctgatcttt cagctgaaca ggtgtcaaac atggttattg cttatgaacc
    43801 aatctgggca atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg
    43861 agttgtacgt caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat
    43921 ccaatacggc ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt
    43981 tgacggtgct ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt cattgttaga
    44041 cgctgtaaaa taattgttga aaatttgcga aaaatgcaag aaatgtttgc actaatgagc
    44101 aaaattaact aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga
    44161 gacaaaacat gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta
    44221 acccaacaat cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc
    44281 cttcaggtgc ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc
    44341 gttatggcgg taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag
    44401 caatcattgg ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag
    44461 acggaactcc taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg
    44521 ttgcacgtgc agctgctgac tacctagaag tacctttata ccactaccta ggcggattca
    44581 acacaaaagt attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata
    44641 actcaatcga cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag
    44701 ctctacgtat gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat
    44761 tagcaacttc tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag
    44821 gttttgaagt aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg
    44881 ttcttgctat ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac
    44941 tagctgattc aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat
    45001 tagtttctaa atatccaatc atctcaatcg aagacggatt agacgaaaac gactgggacg
    45061 gattcaagaa attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt
    45121 tcgtaacaaa cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc
    45181 ttatcaaagt gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta
    45241 aagaagctgg ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa
    45301 tctctgatat cgctgttgca acaaacgctg gccaaatcaa aactggttct ctatcacgta
    45361 ctgaccgtat tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg
    45421 cagaatacaa aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg
    45481 aagatacatg atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa
    45541 ctctctactt gctgaaatat cagcaggtag agagtttttt tgcaactagc ttctgtatat
    45601 gatgtttaca aagaatggta caagaaaaga tcatccttga aaaaggaaag ctaacaataa
    45661 ctagcatcat ataagagctt ttaaggatag aacagtagaa taggaatcta tgagcaagtc
    45721 ctctcgtata aataaaatag gagggaagta gcagcatatt tttcataaag tggctctctt
    45781 tcttcatttt tactttggtt gctcctattt gaaaattcga gcacactcaa atacaataaa
    45841 aagaaatggc aagggagagg attagatgaa aaaagaagaa aaaatcgcga ttttacaaga
    45901 gatcatccga attaaatcag tgaatggaaa cgaaggtgaa gtagcagcct atttgaataa
    45961 gcttttggca aagcatgaca taacaggaga gattgtttct tacagagatg gtagagataa
    46021 tttgattgct cgttaccaaa agggccaatc aggaaaagtg ttaggcctat ctgggcatat
    46081 ggatgtcgtg gcggctggtg atgaatcgtc ttggacctat gcaccctttg cagcagaaat
    46141 ccatgggaac cgtttgtacg gacgtggtgc tacagatatg aagagtggct tggcagcaat
    46201 ggtgattgcc atgatcgaac tgaaagagtc aggaaaacca tttaacggaa cagtcaaatt
    46261 attagccact gtcggagaag aagtgggtga actaggtggg gaacagctca cgaaagcagg
    46321 ctatgtagat gacttagatg ccttgatcat cggagagcca accaattaca gtttgatgta
    46381 tacgcacatg ggatcaatca actatacagt cacctcgcac ggaaaagaag cgcatagctc
    46441 tatgccagac caaggttata atgcaatcaa tcacctaaat gaattcatta cgaaagcaaa
    46501 tgctgaaatg aatcacctcg ctgaaacaat cgagaatcct gtcctaggaa aaacaatcca
    46561 taatgttaca ttgatcagtg gcgggaatca agtcaacagc atcccaagcc atgcgcagct
    46621 ccaagggaac attcgatcga ttccagaata cccaaatgat aaaatcattg cattgctgca
    46681 atcaatcgtc aatgaattga atcaagagac agactatcat ttggaattga tgatcgatta
    46741 caacaaaata cctgtcaaag ctgatccaga ttctcctttg attcattgca ttcagcaaca
    46801 attcagccag ccattacctt tagttggagc ggcagcaaca acggatgcag ccgaatttac
    46861 gaaagctaac cattccttcg actttgtcgt ctttgggcct ggtgtcgtta ctttgcctca
    46921 tcaagtagat gagtatgttg aaatcgataa ttatttggat atgatcgaaa aatatcaagg
    46981 aattatttta tcctacttag cgtaagattt ttttaataaa tgaaaaaaca gagccagatt
    47041 agcattattt
//
LOCUS       DAZKOR010000012        45080 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000012 DAZKOR010000000
VERSION     DAZKOR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.012"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG8ZM_000615"
     CDS             212..604
                     /locus_tag="ACG8ZM_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0491602.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG8ZM_000616"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG8ZM_000616"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0491603.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG8ZM_000617"
     CDS             2698..2943
                     /locus_tag="ACG8ZM_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491604.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG8ZM_000618"
     CDS             3165..4775
                     /locus_tag="ACG8ZM_000618"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0491605.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG8ZM_000619"
     CDS             complement(4752..4964)
                     /locus_tag="ACG8ZM_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491606.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG8ZM_000620"
     CDS             5067..5936
                     /locus_tag="ACG8ZM_000620"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0491607.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG8ZM_000621"
     CDS             6101..7372
                     /locus_tag="ACG8ZM_000621"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0491608.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG8ZM_000622"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG8ZM_000622"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0491609.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG8ZM_000623"
     CDS             8763..9026
                     /locus_tag="ACG8ZM_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0491610.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG8ZM_000624"
     CDS             9471..9920
                     /locus_tag="ACG8ZM_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0491611.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG8ZM_000625"
     CDS             9977..11395
                     /locus_tag="ACG8ZM_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0491612.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG8ZM_000626"
     CDS             11395..12255
                     /locus_tag="ACG8ZM_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0491613.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG8ZM_000627"
     CDS             12429..13202
                     /locus_tag="ACG8ZM_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0491614.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG8ZM_000628"
     CDS             13349..13714
                     /locus_tag="ACG8ZM_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0491615.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG8ZM_000629"
     CDS             complement(13862..15070)
                     /locus_tag="ACG8ZM_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0491616.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG8ZM_000630"
     CDS             complement(15089..15367)
                     /locus_tag="ACG8ZM_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491617.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG8ZM_000631"
     CDS             15560..16216
                     /locus_tag="ACG8ZM_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0491618.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG8ZM_000632"
     CDS             complement(16658..17455)
                     /locus_tag="ACG8ZM_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0491619.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG8ZM_000633"
     CDS             complement(17452..18636)
                     /locus_tag="ACG8ZM_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0491620.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG8ZM_000634"
     CDS             complement(18661..19908)
                     /locus_tag="ACG8ZM_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0491621.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8ZM_000635"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG8ZM_000635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0491622.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG8ZM_000636"
     CDS             21888..22202
                     /locus_tag="ACG8ZM_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0491623.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG8ZM_000637"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG8ZM_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0491624.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8ZM_000638"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG8ZM_000638"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0491625.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG8ZM_000639"
     CDS             23546..23875
                     /locus_tag="ACG8ZM_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0491626.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG8ZM_000640"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG8ZM_000640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0491627.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG8ZM_000641"
     CDS             24863..25687
                     /locus_tag="ACG8ZM_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0491628.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG8ZM_000642"
     CDS             25680..26027
                     /locus_tag="ACG8ZM_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0491629.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8ZM_000643"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG8ZM_000643"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0491630.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8ZM_000644"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG8ZM_000644"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0491631.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8ZM_000645"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG8ZM_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0491632.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8ZM_000646"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG8ZM_000646"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0491633.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACG8ZM_000647"
     CDS             31684..32451
                     /locus_tag="ACG8ZM_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491634.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFIVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACG8ZM_000648"
     CDS             32441..34483
                     /locus_tag="ACG8ZM_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0491635.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="ACG8ZM_000649"
     CDS             34764..34913
                     /locus_tag="ACG8ZM_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0491636.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8ZM_000650"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="ACG8ZM_000650"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0491637.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8ZM_000651"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="ACG8ZM_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0491638.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8ZM_000652"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="ACG8ZM_000652"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0491639.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8ZM_000653"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="ACG8ZM_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0491640.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="ACG8ZM_000654"
     CDS             39330..40412
                     /locus_tag="ACG8ZM_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0491641.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="ACG8ZM_000655"
     CDS             40412..41215
                     /locus_tag="ACG8ZM_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0491642.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="ACG8ZM_000656"
     CDS             41285..42025
                     /locus_tag="ACG8ZM_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0491643.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="ACG8ZM_000657"
     CDS             42036..43040
                     /locus_tag="ACG8ZM_000657"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0491644.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="ACG8ZM_000658"
     CDS             complement(43085..44929)
                     /locus_tag="ACG8ZM_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491645.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14514 a         7845 c         9370 g        13351 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgagggtga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc atagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga
//
LOCUS       DAZKOR010000013        44823 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000013 DAZKOR010000000
VERSION     DAZKOR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.013"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..1013)
                     /locus_tag="ACG8ZM_000659"
     CDS             complement(3..1013)
                     /locus_tag="ACG8ZM_000659"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0491646.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1193..1537)
                     /locus_tag="ACG8ZM_000660"
     CDS             complement(1193..1537)
                     /locus_tag="ACG8ZM_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0491647.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1530..1964)
                     /locus_tag="ACG8ZM_000661"
     CDS             complement(1530..1964)
                     /locus_tag="ACG8ZM_000661"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0491648.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2102..2839
                     /locus_tag="ACG8ZM_000662"
     CDS             2102..2839
                     /locus_tag="ACG8ZM_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491649.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2832..4043
                     /locus_tag="ACG8ZM_000663"
     CDS             2832..4043
                     /locus_tag="ACG8ZM_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0491650.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4247..5029
                     /locus_tag="ACG8ZM_000664"
     CDS             4247..5029
                     /locus_tag="ACG8ZM_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0491651.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5099..5761
                     /gene="trmB"
                     /locus_tag="ACG8ZM_000665"
     CDS             5099..5761
                     /gene="trmB"
                     /locus_tag="ACG8ZM_000665"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0491652.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5859..7613)
                     /gene="ade"
                     /locus_tag="ACG8ZM_000666"
     CDS             complement(5859..7613)
                     /gene="ade"
                     /locus_tag="ACG8ZM_000666"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0491653.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7745..8674
                     /gene="comGA"
                     /locus_tag="ACG8ZM_000667"
     CDS             7745..8674
                     /gene="comGA"
                     /locus_tag="ACG8ZM_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0491654.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8631..9680
                     /gene="comGB"
                     /locus_tag="ACG8ZM_000668"
     CDS             8631..9680
                     /gene="comGB"
                     /locus_tag="ACG8ZM_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0491655.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9677..9955
                     /gene="comGC"
                     /locus_tag="ACG8ZM_000669"
     CDS             9677..9955
                     /gene="comGC"
                     /locus_tag="ACG8ZM_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0491656.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9930..10400
                     /gene="comGD"
                     /locus_tag="ACG8ZM_000670"
     CDS             9930..10400
                     /gene="comGD"
                     /locus_tag="ACG8ZM_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0491657.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10372..10719
                     /locus_tag="ACG8ZM_000671"
     CDS             10372..10719
                     /locus_tag="ACG8ZM_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491658.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10682..11107
                     /gene="comGF"
                     /locus_tag="ACG8ZM_000672"
     CDS             10682..11107
                     /gene="comGF"
                     /locus_tag="ACG8ZM_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0491659.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11079..11438
                     /gene="comGG"
                     /locus_tag="ACG8ZM_000673"
     CDS             11079..11438
                     /gene="comGG"
                     /locus_tag="ACG8ZM_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0491660.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11610..12617
                     /locus_tag="ACG8ZM_000674"
     CDS             11610..12617
                     /locus_tag="ACG8ZM_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0491661.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12642..13826
                     /locus_tag="ACG8ZM_000675"
     CDS             12642..13826
                     /locus_tag="ACG8ZM_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0491662.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14263..15846)
                     /gene="abc-f"
                     /locus_tag="ACG8ZM_000676"
     CDS             complement(14263..15846)
                     /gene="abc-f"
                     /locus_tag="ACG8ZM_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0491663.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15951..16079)
                     /locus_tag="ACG8ZM_000677"
     CDS             complement(15951..16079)
                     /locus_tag="ACG8ZM_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491664.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16424..16567)
                     /locus_tag="ACG8ZM_000678"
     CDS             complement(16424..16567)
                     /locus_tag="ACG8ZM_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0491665.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16790..17269)
                     /locus_tag="ACG8ZM_000679"
     CDS             complement(16790..17269)
                     /locus_tag="ACG8ZM_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0491666.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17342..17626)
                     /locus_tag="ACG8ZM_000680"
     CDS             complement(17342..17626)
                     /locus_tag="ACG8ZM_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491667.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17704..17898)
                     /gene="ssrS"
                     /locus_tag="ACG8ZM_000681"
     ncRNA           complement(17704..17898)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8ZM_000681"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17970..19256)
                     /locus_tag="ACG8ZM_000682"
     CDS             complement(17970..19256)
                     /locus_tag="ACG8ZM_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0491668.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19394..20065
                     /locus_tag="ACG8ZM_000683"
     CDS             19394..20065
                     /locus_tag="ACG8ZM_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0491669.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20031..20519
                     /locus_tag="ACG8ZM_000684"
     CDS             20031..20519
                     /locus_tag="ACG8ZM_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0491670.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20532..21479
                     /gene="prmA"
                     /locus_tag="ACG8ZM_000685"
     CDS             20532..21479
                     /gene="prmA"
                     /locus_tag="ACG8ZM_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0491671.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21480..22244
                     /locus_tag="ACG8ZM_000686"
     CDS             21480..22244
                     /locus_tag="ACG8ZM_000686"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0491672.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22477..24690
                     /locus_tag="ACG8ZM_000687"
     CDS             22477..24690
                     /locus_tag="ACG8ZM_000687"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0491673.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24701..25147
                     /gene="dtd"
                     /locus_tag="ACG8ZM_000688"
     CDS             24701..25147
                     /gene="dtd"
                     /locus_tag="ACG8ZM_000688"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0491674.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25318..26577
                     /locus_tag="ACG8ZM_000689"
     CDS             25318..26577
                     /locus_tag="ACG8ZM_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0491675.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26666..27613)
                     /locus_tag="ACG8ZM_000690"
     CDS             complement(26666..27613)
                     /locus_tag="ACG8ZM_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0491676.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28007..29299
                     /gene="hisS"
                     /locus_tag="ACG8ZM_000691"
     CDS             28007..29299
                     /gene="hisS"
                     /locus_tag="ACG8ZM_000691"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0491677.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29314..31083
                     /gene="aspS"
                     /locus_tag="ACG8ZM_000692"
     CDS             29314..31083
                     /gene="aspS"
                     /locus_tag="ACG8ZM_000692"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0491678.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31192..32067
                     /locus_tag="ACG8ZM_000693"
     CDS             31192..32067
                     /locus_tag="ACG8ZM_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0491679.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32333..34216
                     /locus_tag="ACG8ZM_000694"
     CDS             32333..34216
                     /locus_tag="ACG8ZM_000694"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0491680.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34291..34575)
                     /locus_tag="ACG8ZM_000695"
     CDS             complement(34291..34575)
                     /locus_tag="ACG8ZM_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0491681.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34565..35299)
                     /locus_tag="ACG8ZM_000696"
     CDS             complement(34565..35299)
                     /locus_tag="ACG8ZM_000696"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0491682.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35428..36051
                     /locus_tag="ACG8ZM_000697"
     CDS             35428..36051
                     /locus_tag="ACG8ZM_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0491683.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36243..37364
                     /locus_tag="ACG8ZM_000698"
     CDS             36243..37364
                     /locus_tag="ACG8ZM_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0491684.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37371..40499
                     /locus_tag="ACG8ZM_000699"
     CDS             37371..40499
                     /locus_tag="ACG8ZM_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0491685.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40518..43712
                     /locus_tag="ACG8ZM_000700"
     CDS             40518..43712
                     /locus_tag="ACG8ZM_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0491686.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43858..44565)
                     /locus_tag="ACG8ZM_000701"
     CDS             complement(43858..44565)
                     /locus_tag="ACG8ZM_000701"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0491687.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14381 a         7961 c         9406 g        13075 t
ORIGIN      
        1 ttttattttg atgaatcagt tgtttcttca gttgaagatt cttttgttga atcagttgat
       61 tttgtgtcag aactctttgt tgaagctgta gtttcacttg agtctttcgt acttgatgaa
      121 gttgttgtga atgcactcaa cacattttca aaggcatcat ctttgatttt tacatttgca
      181 tcttttaatt cttcaccgat tactttagtt gtgaatgcat tgtcacttag tttagtttca
      241 gtagcgatat cttttagttg atctttgtat ttatccatgt cattgccttt attttggttt
      301 ttcaccattt tgacaacgta gtattctgtc gcataagaag taggatttgt tgtagtaata
      361 acatcggaga tctcaccgtc tttcaatttg aaagctgctt cttttacttc tgcaggaata
      421 gttgttgtcg ttgagtcgaa tttaacttta ccgccatctt cttttgtctc tgtatcagtt
      481 gatttgtctt tggctaattt agagaaatca tctccatcat ccgctgattt tttcacatct
      541 tttgcttcat cttcacttga taatttgatg atttgtgctt ctacttctgg atggaaagat
      601 ttccaagcag ttttcaaatc gtcatctgta atatctacgt gagctttcaa gccagcttca
      661 aaagctaaat tgttacgaat atattcttta taagtatctt tcgtatatcc agcagcttct
      721 aattgtgatt caaaagtatc acctaatgat ttcgcttgtt tgtcgtattc tgcatccact
      781 tgcttatcgg ttactttatc gccatatttg ttattgaata ctttataaat gatcatccgt
      841 tgtactaagg attggttcga tgattcaagt tttgcttcat catagaaatc agatacagtg
      901 atcgttccgc ctttcatcgt tgcgatatct ttattagtat cgcctgaaca agctgctaga
      961 gttaaaacag ccaacgcact agtagctgcc aaaataattg attttttttt cattaaaatt
     1021 tgcactcctg taattagtta ttatttttat atattaacta taccatatcc catttccttt
     1081 aaaaacgttg tcgtggtagt ttcaaacaaa attcaaaatt tcatcacatt cctaggaaat
     1141 acgcaaactt ttctcactta tatagttaaa tctgttgaga aaataatcat tattatattt
     1201 gttcaggata ctctggcaga tcagctttta gtttttctac ttgttcattg atttgtgtga
     1261 tacgtggttc ggcttggaat ttaaaatctt cgatatcttt tttgattgct tcttggaaag
     1321 caggaatcac agattcagct gtttcttttg tttcgatcaa tgcgctttta aaatgattca
     1381 ggctgttatt taaatcattt gtcagagttg ttgcttcttc caattcttcg atcagtttct
     1441 ttcttgtttc attcccgctc ctgggcgcca ataacagacc tccgacagtg ccagcagcag
     1501 ctccaaataa cagtcctttg aaaaagtttt taatcatcgc aggacacctg ctttacgatt
     1561 gtttcttgaa tagcttttag ttcttccgat ccataactat cttgatgatt gacaaagtga
     1621 atagaaaaat catcctcttt gctgtagcga gggactaaat ggatatgtga atgaaaaaca
     1681 gattgatagg ctagttcacg gttgttattc aagatattaa gacctttcat ttctggaaaa
     1741 gctttttcca atgcttgagc aatcttaggc acacgcgaaa aaacctctcc tgcaagttcg
     1801 ggatcatatt caaaaatatc tgctacatga cgtttaggca caactaatgt gtgtcctttc
     1861 gttgcttgag agatgtctaa aaaagcatag accttgtcgt cttcatatat tttatagctt
     1921 gggatttctt gattgatgat tttgcaaaaa atacagtttt ccatataaaa catcctttct
     1981 ataaagaata aatacttcag cttaacttta ccataagtta aagctattgt aaggaaaaaa
     2041 acaaaatacc gtcattttct tcgtggtata ataaataaaa ataaatggcg gaggaaaaag
     2101 aatgagttta attatcgatc atgtcacagg ggggtacgga catctccctg tgttaaaaga
     2161 tatcagtttc gatgtacagc ctggagagat agtagggttg attggactga acggagcagg
     2221 gaaaagtacg accatcaagc acattatcgg tctgctggaa gcttcaaaag gaacgataga
     2281 aatcaatcat ctatccttaa agaaaaatac ggaagaatat cgcaaacaaa ttgggtatat
     2341 tccagagact ccttctttat atgaagaatt gacattgaga gaacatatcg aagtcactgc
     2401 gatggcctac gatataccta tggatatagc aatgaaaaga gcagaaaagt tgttggagac
     2461 ttttcggtta tctaatcgtc ttgactggtt cccagctaat ttttcaaagg gaatgaaaca
     2521 gaaagtgatg atcttatgcg cctttttgat cgagccttct ttatatatca tcgacgaacc
     2581 atttttaggc ttggatccac tagctatcaa tgcattgctt gaattgatgg tagaaatgaa
     2641 aaaagaagga gccggtattt tgatgtctac gcatatttta gctactgcag aaaaatattg
     2701 tgatcgtttt gtggtcttac ataacggaga gatacgagca gacggttctc ttgagatgtt
     2761 gagaaaagaa tttcagctgc cggattcttc tttggacgaa atctatattg ctttaacaaa
     2821 ggagcaggca catgagtagt ttttttcaaa aacgtttagc aagacatcaa aagaaaatga
     2881 tgaaatatat gcgttatgta ttgaacgatc attttgtttt agtttgtctt ttcttgattg
     2941 gcggtgtggg attatattat tctaattggt tgaaaaccct gcagccaccg tttgcaattg
     3001 gagggctcat aatctctgta ttttggatgc tctgtttatt cgtgggtaag atggctactt
     3061 ttgcagaggc agcagatatt gtttttttac ttcctaaaga aaaagaaatg caaacctatt
     3121 tggaacgagc ttttcgctat tcttgcattt ttccgttgat ctttttgata ttggcatgcg
     3181 gattggccat gccattagtg gtggttagta cggagcaatc cttttcgatg tttttcgtct
     3241 atctgatcat attatggagt ttgaaattca gtcatctgca aatcaagaga gcagaattgt
     3301 ttcgactaga tcaacaagtt attcgttatt ggaaattagg ctggtttttt tcttctctga
     3361 tctttttgtt tctatcattt tattggctaa tatgggcagg aatagcaggt gcattagtac
     3421 aggcaagtct atattactgg tttttgtgga agcaacaaga aaaaggattg gattgggaga
     3481 aaatggtgca tgtagaagaa cataggttgc atcagatcta tcaatttatc aatttattca
     3541 cagatgttcc tgaaattacg gcaaagatca aaagaagacg ctatttggat cctatattga
     3601 ctcatattaa aagagaatca aaaaacactt atctttactt gtttgcaaga agattggcaa
     3661 gaggttctga ttttagtggt ctttatttcc gattagtaat tataggtggg ttattgattg
     3721 ccggagtagt cgacttttat tttagtctga ttatcggttt cttattttta tatttgatcg
     3781 gtttccaact gttaccttta gcgaaccagt tccgctatat ggttctcgtt cagctttatc
     3841 caatccataa gaatcagaag aagcaggcga ttcagtatct actcacatgg gtattaatta
     3901 tcgcgtcggt tatttttggt ttgattgctg cagtcgtatt agatgggcag cagcgatggg
     3961 ttccgctagc ggcttatttg attgttacta tgctctttgt taaactatat gtaccaactc
     4021 gtttgaagaa gatgtcagac tgaaaacaaa gctgtgtagg gtaaaaatga gaatgaaaaa
     4081 tagagcgttg acagtaagaa tgctgttcgt tctatttttt ttgagaaaag ctggtttttt
     4141 gaatggcaat taaatgaaaa agaacgtata tgtgcttgac gtgtagtgtt caggcgacta
     4201 gaataataag tactgcaaaa caaagagtaa gaggacggga gaatgcatga tggagtttca
     4261 gttagaccaa gagtggcggt tacgtcccat caaaggcgac acaggaaaaa catacatagg
     4321 catgcgtgat gaggacaaag tctttattaa acgaaacacg actccgatgt tggctgctct
     4381 ttcaagggaa ggaattacac caaaacttgt ttggacaaaa cgaacgggga atggcgatac
     4441 tttgacagca caagagtggc tggaaggtcg cttgctgact cctaatgaga ttggtcaaag
     4501 aaacgacgtg attgatgttt tgtaccattt acatcattca aattctctta aaaccatgct
     4561 gacccgtatc ggtggtcgtg tcatgacgcc ggagaagatg ctgcaaaaat atgaaaaaga
     4621 attaccggaa gaactccaga aaaatcaatt tctgcaaatt gtccatcgtt ttcttcgtga
     4681 gcagatcccg tcaatagata aggaaaactt atcagtagta cacggagatg taaaccatcg
     4741 taattggtta gtatgtaaaa attatttata tctagtcgat tgggattcaa tcatgtttgc
     4801 agaccctgcg gtggatattg caacgatttt aggaaattat gtgccactgt ctagctggaa
     4861 tcagtggctg atcagttatg gcatacgccc gactgacgaa atgcttgaaa aatttcattg
     4921 gtatgcggcc atgaatttgc tccaagaaat caccagatat tgcaaacgtg aagatcatcg
     4981 acggatgaac gaagaaattt tacaattaaa acgaattttc agtggctgag gtttggccca
     5041 aactctgcgg agatttggcc agaccttttt aattagagga aaggaatgga gataaataat
     5101 gcgtgtccga aatcgtccgg gagcagcgga aatgttagcg gctcacccga attttgtaat
     5161 tagtgatcca acattgtgga agggaaaatg gaatgaattg tttgaaaacg atcatccaat
     5221 ccacattgag attggtatgg ggaaaggcca gttcatcact ggaatggcaa aagcccatcc
     5281 tgaaatcaat tatattggag tagaaatgca ggtcagtgtt gtttcgattg cactagacaa
     5341 gttgatcgaa caaccgcttc ctaacctgaa acttctacat gtggatggat cagcgttgac
     5401 agagtatttc gctgacagcg aagtagatca aatctatctg aatttttccg atccatggcc
     5461 aaaaaaacgt catgaaaaac ggcgattgac atataagact tttcttgctg tagatgaaca
     5521 gattcttcgt ccaaatggag aaatccattt caaaacagat aatcaaggat tgtttgagta
     5581 ttctctggca agtttttctc aatatggcat gatcctcaaa caggtatggt tagatttaca
     5641 tcaaagtcaa tttgaaggca atattatgac ggagtatgaa gaaaaatttt cttcaaaagg
     5701 tcaacgtatt tatcgagtag aagctcaatt tcaagataag accaaaaaga aaagtctgtg
     5761 aaactaactt tctgctttca taaaaacatt aatatccata aataaatccc agaaaatgaa
     5821 cattacagat gtaatgtatt ttctgggatt tatttgattt aaaatgtatt tatttcatcg
     5881 gtttttccac aaggtggaat caattggaag cgagaaaatg aaaataatcc tttgtcggtg
     5941 atcttcaatt cgggtatgac agataatgtt aagaaagaaa gtgtcaaaaa aggatcaaag
     6001 gttttagctg ctcctagttg atgtgcttta gttattaatt cattcaactg ttcatagacg
     6061 atcgaaaaag gttcctgtgt catgattccg cctataggaa gagaaacaca agctatttcc
     6121 tgctgattgt tgacaacggt catcccgcca cctttttgaa tcaaaagatt agcagcatac
     6181 agcatatcct cgtcgtttgt cccaacgaca actagattat gcgagtcatg ggcaatcgtt
     6241 gtcgctatcg ctccttgttt cagctgaaat cctttcacta tgcctaatcc aatgtttccc
     6301 gtttgatggt gacgctcgat caccgcaatc tttaattggt ctttttcgat tgaaggaatg
     6361 aatcgttcgc cttgtcggtc cactttttct accaaatctt tagtcactag actattcggg
     6421 ataatttcga tcacatgggc ttgtgaagaa gaaagaagca gctctaacga atgtggattt
     6481 aatggttgag cgtttagttt tggcagacaa cacgtatatg aatttgaatg atcagcaaat
     6541 aaatggtctt ggagttgccc agattgaaca actaatgttc ccttatagaa aacttcatga
     6601 attggcagct tggtaagatc gtctgttaag aagaaatcag cttgataccc aggggcaatc
     6661 gcacctagtg ttttcaaatc aaaacattct gctgcattca atgttgccat ctgaatggct
     6721 tgtaatggat cgattccaag tgcaatagct tgacggacga tatgatcgat tgatccttct
     6781 tttactaaat cattgatcag tttatcatcg gtgacaaaga agcatcttct tgaattttca
     6841 ggtgtgatcg ccggtagtag cgatgccaga tcttttgcaa cggtcccttc tcgaaccatt
     6901 aaatacatac caagatccaa acgttcttta gcttcttttg cagttgtagc ttcatgatcc
     6961 gtccgaatac ctgctgcagg ataaacattt aactcttcca tgccgatacc agctgcatgc
     7021 ccgtcaattt ttttcttatg ctggtgcatc aattggatct tttctaggat gtctgtttca
     7081 ttatttgcta ctgcttgata gttcatgact tcagctaagc ctaaaacttc gggtttttgt
     7141 aaaaaagggg ctaatttttt tgcatttaaa tttgcaccat tggtctcaaa aggggttaca
     7201 ggtacacaag aaggtagcat aaaaaagata ttcatgagtg tttgtcttgc atcttctatc
     7261 ataaactcaa gacctgtact cccagcaatg tttcctattt catggggatc tgtcactaca
     7321 gtagtaactc catgaagcaa ggatactttt gaaaattcgc ttggtgataa aagtgagctt
     7381 tcgatatgca catggccatc gatgaatcct ggcactagat attgcccatt agcattatgt
     7441 acgctttttc cttcgtattc accgattcct acgatcattc cttcgcagat tgctacatct
     7501 ttttccatga tttctttcgt aaagacgttg acgatcttag catgcttgat cactaggtca
     7561 gcaggctttt ttttacttgc tacttcgata tatttcctta atgttgtttc caatggagtg
     7621 cctcccaaga taaattttta taagtataac ataaattcgg tttttgccaa tatttttttg
     7681 taaattcatt ttatttgaaa caaaaagatc aaaaagacgt atgttttttg tggaggggag
     7741 aataatggaa ataaaacgat tatcggatca aatgataagt gaaagttttg ccaaacatgc
     7801 ccaagacgta tatatattgc cagcttcaga agtattttac atctattttc gctatggaaa
     7861 aaaacgaaca ttctggaaga aagtctcttc ggcagatgga cagcaactga tctcaagatt
     7921 taagtatttg ggacagatgg atatcggaga aaaaagaaaa gctcagttag gtgcatttac
     7981 ttattcttta gaaaaacagg aagttcgtct gcggttgtct tctgtaggag attaccttca
     8041 acgagagagt ttagtgattc ggctattata cggtactttt gaaacacccc atcgttgctt
     8101 tagttctcga gattttgcaa ctattttaca gaaaaccgtc agacgtggat tatacctttt
     8161 cagcggacca gtcggatcag gcaagacttc tctaatgtat cggttggctt tggaagaaaa
     8221 attgcaagtg attactatcg aagatcctgt agagatagaa gaaccgtttt ttctgcagct
     8281 acagatcaat ccgaaaatag cacagactta tgatacactt ttgagactcg cattacggca
     8341 tcgtccagac ttgctaatca ttggcgagat aagagatgaa tcaactgcac atgctgcggt
     8401 aagagcagca ctaacagggc accgggtttt tgcaaccgta catgcacgca atcttgaagg
     8461 tacattggtc agaatccatg aactgacaaa gaaaaaagaa gagctatctg aatgtttagc
     8521 tggtgtcatt tatcaagaac ttttggaaga tgccaatcac catccttctg ttttatggag
     8581 ttatcaattt atggaggaag aatcagtaaa aaaatcatgg gaggaaagtt atgaagaagc
     8641 tcaggaaaat atttggcagt cagaactatt ctaaaggatt aacaagaagc cagcaaaacc
     8701 agtttgcaca aaccatggga gaactgcttt tatctggttt tagtctgcaa gaatcccttt
     8761 atttattact cacgatccgt tcgtttccac ctgttatttt ggcaaatatc caacatcagc
     8821 taaaggaagg gcaagcgttt agtgaggtac tttcgcaaat aggatatgga gcagagcaac
     8881 tggtacagat cgagttagct gaacgtcatg ggaatctgcc gacaactttg ttggaaattg
     8941 ccaaacagtt tcggcttgtt gaaggatttc gcaatgattt gaaaaagctg cttgcatatc
     9001 cgatgctatt gctcaccttt ttgctaggta tcttgtttag tatgcggttg ttcgttttgc
     9061 ctcagcttat tggttcagga atggtcatgc aagagcattg gggtattcag ctgatacaag
     9121 tctttcattg gtatgttcta ctattcatcc tagcggttac tctattggct ttactagtaa
     9181 ggtatcgtct gatgaagttg tctcctgttg atcagagtga atggataagc agacaattat
     9241 tgattggacc gatatattcc ttgtatcaat cggcttattt gtcactagag attggaaagc
     9301 tttttcatga aggtttcgaa ttaaggcaaa tcatagtctg cttaaaggaa acgaaacaag
     9361 aaagtctgat tcagcatctt gcctctaagt tgattcaagg attggaggca ggtatgtcat
     9421 tggctgagca gttccagaat tatcgttttc tatcagaaga attcagtaaa attgttttac
     9481 agggagaggc aaagggaagt ttaggtaaag aactgatttt ttacagcgaa tggacaagaa
     9541 aacagctttt caaacgtctt catcactgga tgcattttat tcagccaatc atttttttgg
     9601 gcgtagcggc tttgatttta ttagtttatg ccgctgtttt attacctgtt tacggaagca
     9661 tagaggaggt tttgccatga aaaagagaaa aatatcttat tatagcggtt ttacattgat
     9721 cgaaatgcta atcgtcttac tgatcatcag tgtattagtg ttgttgttcg tccctaattt
     9781 gtcgcgttat aggaatcatg tagaccaaga aagtcgtgaa gcgatcatcc agttggtaga
     9841 cacacaaaaa gagctctatg cgcttcaaaa taatggaaga gttcctactg tggaggaatt
     9901 gttgaatgaa ggctatatca aacgagaaca tgcagaaatc tatcagcggc cttgaaggta
     9961 aggttttagc atcaggatat acgttgtttg aatcactctt actgatttta ttacttacaa
    10021 cgatcctttc ttttccagtt ctcgcatttt ctgcatggaa gaatgaattg gcagtctatc
    10081 aatttttcgg ccaatttgaa aaacgaatct attctacgca aaaaatcgct attgtgaatc
    10141 agatccaaac aggcttttat tggaatagtg aagagaatca aatcattttt catgttccga
    10201 atccagataa aataaattgg gaagtattag atattcctga tgagattacc ttgaaacgcc
    10261 atgcttctat tacttttgct gctagaaccg ggaatgaaag ttcactaaaa gcttatcaat
    10321 tttattggga agaaaaagag cggacgatta cgtatcaatt ccagataggg agtggtcgct
    10381 atacaaaaag aattgaataa aacaggattt gttttgttgg agtcccttac agcgcttatg
    10441 atcgcttctg tctgtgtcta cttagtgagc tttggccagc tgtttttgct tcaggcagaa
    10501 aagaaaagtg gggaggaact gctatttatt cgaatgattt atgaagatat caagacgcag
    10561 cggatgtatc acaaaagacc agagcgaagg gaagaaatga tcccatacga tatccaaatt
    10621 gtcgataata atggagtaat cgaaaaagtg tttgtcaata tggagaaaga agcgatcagt
    10681 attgaaaaaa caagtaattc cagcatttac gctgattgaa tgtctaattg gactgatgat
    10741 attgagcagt ctattgctca cattcaatct tttgattcag caaaccatcc aagtaagtga
    10801 atttttgcag cgtaaagatc aaaaagagtg gctaatattt ttagcacaat tagaagaaga
    10861 actcaagagc agtcacaatg tctctgtgaa aaatcagcaa ctgcactaca cgatcgagga
    10921 taaacagtat atcatcgaaa gatatcaggc aatgatacgt aaaagaagaa cttccggcgg
    10981 acatcaacct atgttaacaa gtatcactga gcttcaatta ttggaaaaag aacacacaat
    11041 ttctttttat gtacactttg aaaatggaga agatggatat gccatatgga caaaaaacga
    11101 tcagtagaaa atgttataag ggctctattt tattttctgt cttgctttta tttcttttat
    11161 gtacgttttt attgatggca gcattagaaa gctaccgttt gtcaatggat cttaatatac
    11221 gaatcaaaaa ttattatata gcaaaaataa tgaagcagat ggctttatcg gaacagtcta
    11281 cgcttgccga aagttcggaa ggagtttttt actacacaac tggatctgta acatatcagt
    11341 gggtacaaca gtccctattc ttagaggtgg aagtctcacc gtttatcttt cggttcacag
    11401 aagaagtaaa aaatgataca gatactagta cagaatgatg gaatgttcgt gaaaacgccc
    11461 tcttttctgt tataacactg tttttaataa aaaaatgttt gccgtttcac aaataaaaaa
    11521 cagtgaaaac gattgaaatt ttgtcaattg ttcgatagaa ttaaacaggt gaaaaaattg
    11581 ttataatata tctagaaatg aggtgcacca tgttaccaga aaaaatggaa caagggttta
    11641 atcaaaacct ggaagctatt cagcttttac agaatgcatt gggaacttcc tttttagaag
    11701 cctatgtcga aaatgcagaa aatttaatag atgattatca agtacgtgtg gtggatggag
    11761 tgccgacaaa agagacaacg caacggatca cagctcttta tgaagagttg aagaaacttt
    11821 ctttcgagcc agaagaatgg cgcagactgt ctcaattact tttattgaag ggcagccaga
    11881 cagaacattt gcaggcgaac catcaactca ctcctgatag tatcggtttt ttatttgttt
    11941 ttcttattga acaactatac acaaataaaa aagcaccggt taagattctg gatattgcgg
    12001 caggaatggg caacttgtta ttaacagttc ttttaaacct atcgaatgcg gggtaccaaa
    12061 cagaaggaat tggtgtggat attgatgata cattgttggc tgtcgcagct tctacaagtg
    12121 atttgacaca agcaaatgtt caatactttc atcaagacgg gttgcaggaa ttattaatag
    12181 atcctgtcga ttttgcaatc agcgatttgc caatcggtta ttatccgaat gacgaaaaag
    12241 caaaagagtt tttaacaagt accgaagaag gccatagtta tgcacatcat ttattgctag
    12301 agcaatcaat gaaatatgtg aagccggatg gttttggttt gtttttgatg ccgagtggat
    12361 ttttagaaac ggatcaaagt gaagaaatca aaaaatggtt taaagaagaa ggttatcttc
    12421 aaggaatgat tcaattgcca gatgaattgt tccgtaataa acaatcgcaa aaaagtattt
    12481 tgatcttaca gaaaaaagga ccacaagcac aacaagtaaa agaagtcttg cttgtgaaac
    12541 ttgcttcatt aaaagaacca gagaaggtga cggaattttt caacgagttt aaaaactgga
    12601 aatcatctgc tctgtaatca ttcaacgaga aggagagtca tatgtctaaa acaattgcaa
    12661 tcaatgctgg gagttcaagt ttgaaatggc aattatatca aatgccagaa gaaaccgtag
    12721 ttgcgaaagg gatcgtcgaa cgtatcggtt tacaagattc tatttttacc atcaagtacg
    12781 gcgataatca aaaattcgaa caaatcatgg atatcgaaaa ccacgaagta gcagtaaaaa
    12841 tgctgttaga tcagttgatt gaactgcaaa ttttaagctc ttatgatgaa atcaccggag
    12901 tagggcaccg tgttgttcac ggaggagaaa cttatgggga ttcagtagtg atcgatgaga
    12961 aagtaatgga ccgcattgct gaattagcag aatttgctcc tttgcataat cctgcaaact
    13021 taatggggat caaagcgttt aaaaagattt tacctgacat tttgagtgtg gctgtattcg
    13081 atacatcttt ccactcaaca atgccaaagc aaaattattt atatagctta ccggtgtctt
    13141 attatgaaga tttcaaagta agaaaatatg gcttccatgg cacaagtcat cgttatgtgt
    13201 cggaacgtgc ggcaaaatta ttgaacaaac cgatcgaaga actaaaaatc atcacttgcc
    13261 acttaggaaa tggtgtatcg atcacagcag tcgatggagg taaatccatg gatacttcta
    13321 tgggattcac accattagct ggggtgacaa tgggtacacg ttcaggtgat atcgatccag
    13381 ctgttctccc atatttaatg gaaaaattag agattgatat tgatgaaatg atcaatgttt
    13441 tgaacaagaa atctggtctg ctaggcctaa caggattgtc tagtgatatg cgtgatcttg
    13501 aaaagaacta tgaaaaagaa catattcgtt tagcttatga cattttcgtg gaccgcatcc
    13561 gaaaatacat cggcggttat gtgacaacga tgaatggtgt tgatgcaatt gtctttactg
    13621 ctggtatcgg cgagaatgac ggacatgtac gtagtgaaat catcaaaggt atgtcttggt
    13681 ttggttgtga aatcgatcca gcgttgaatg aattgcgtgg tgaagaagta gatatctcta
    13741 caaaagaatc aaaagtaaga gtattagtga tcccaacaga tgaggaacta atgattgccc
    13801 gtgatgtgga gcgtctgcgc ggctaagcca aagcattcgt taggataaaa gatgaaacgc
    13861 atgcaagagg gaattcttgc atgtgttttt tttgcggaga agaccgcaaa atggtaattt
    13921 aacttcaatt tcccttcttt tttacaggat cttcttaggg aattttgctt ggaaaagtgg
    13981 tcaaaaggga aaagactggc attttattta ttgattttca ggaaaatccc aatattttat
    14041 tttcattcat ctttataagg gacattacat atgtaaaaag aaaaaacatt tttagatcag
    14101 cctcgtgggc tgtcgacttt attcttcgct ttatgtttag ctagaaaacc gatccgaaac
    14161 attcattgat cgaataataa aaacttgatt ttccacctgt actttgcctg aatcgtgaaa
    14221 gcacaggtat gaaaatcaag ttttttgttg cgaaaaaacg tcctagtcaa tcagttggcc
    14281 gagcggctgg cggctgatct gctcctgatc aataacgagt gcttcttgga agtgttgctg
    14341 caaaaagtag cggtcatggc tgactgctag gagcgttcct tgatagtctt gcagcatttc
    14401 ttcaatttct tctcgagcat agctgtcgag gtgattggtt ggttcatcca aaatcaggac
    14461 gttgattttt tctaaaaaga gtttcagcaa cgccaaacgg attttttcac caccggataa
    14521 atctttgatc cgtttaccca catcgctttg ataaaagcca cttttggcta attgccgtcg
    14581 agcgacttct tcgtttccag tcaatgtgat ggcgtaagcc agcaaacggc tatcgggatc
    14641 ggcaaactgc agctgctgcg gcaaataacc gatttgcaag ctactaccta atttgatggt
    14701 gccaccgtaa ggcttttcct caccgagtaa taatttgagt aaggttgatt ttcctgcacc
    14761 attgtcgccg atgatggcta aatgatctcc acgatagact gcaaaactgc tgtctttgaa
    14821 tagtacttga tcagcgtact gctggtgcaa gttttgaatg atgaaaactt ctttgccaga
    14881 ttttccacca ttagacaagc tttgcagccg atttttcggg ggaggtggcg gtttgacgag
    14941 tgtcagtttc gccaggcggc gttccagttc tttggctttt ttgaagaagg actcattgtc
    15001 tccttcatgt gcccactgac gaaaacgacg aatcatgact ttcaaccgct ggacttcttt
    15061 ttgctgcagt tcgtaatttt ttgtcagctc agccaagcga gcttctttca agactcgaaa
    15121 acggctgtag tttccgggat actcaatcaa tgcaccttct tccagttccc atgtttttgt
    15181 ggtgacttga tcgagaaatt gccgatcatg agaaacgacc agaaccgatt tttttgtatt
    15241 ctgtaaatag ttttccaacc agcgaatact gttaagatct aaatgattgg tcggttcatc
    15301 gagtaaaagt aaatcggcat cggctgctaa aattttggct aaagcgactt taactcgttc
    15361 gcctccgcta agttctgtta accgtgcttc ctgacggtgt ggaatcccta agccttgcaa
    15421 gatcgctgca atccggtctt ctaatacgta accgcctagt tcttcaaagc gatgctgcag
    15481 ctcaccgtaa cgggccagac acttctctaa gtttgtatct ggttctgtca tcaatgcttc
    15541 gtagcgactc atttgctgtt tgaggtcctg tatctcagcg gcagaatgga gcaagtagtg
    15601 gttgacggtc gcttcggaaa ctgtaagttc ttgttcgacc gtcccgatct ttaagccttt
    15661 tttgcgactg ataacaccgc tgtcgacacc ttctctgccg gtcaagattt gtaaaaaggt
    15721 tgatttgcca gttccgttga taccgactaa gacaattttt tcaccagctt caatttgaca
    15781 ggataaatcc tcaaataatg gattcccgcc gattgttttt ttaatagaaa ttgcttgtat
    15841 gatcatgatc tctctccttg agaccactca aaaaggccat ggttatccat ggcctgtgtt
    15901 atttaaactc agattatcat gagaaaaata ccttgagatg gccattgatt ttacgtgaat
    15961 tcaactgttt tggacaaact tgtcccgtca acagcaactg taaaaccaat tgcatggcta
    16021 aatgcgatat ggtatcgttg gtaattttca ttacttgtta ttttagtcat gcgcatcata
    16081 gtattttccc tccttcgtgt agttgtcttt tccagtgtac ctgaaagtgt cagaaagttc
    16141 aactagcgct gacatttact taatatttat cgatgtgtaa ttctgtcaga gagctttttt
    16201 tatctgactg agggtaatgt ctcgtggacc acatcattgt attctgagct aaaccagcgt
    16261 tcaggcgaaa tcatatggtg tttgataaaa aactttgtct acatgacttc cttttgccat
    16321 ttgcttaata ccgttgcatt aaacagctct tctctttgga aatctcacca acatcgcgtt
    16381 caggagtatc gttgtggttg caatggcaag cggtagcctt tcatcaattc ttgaatgatt
    16441 taccgaatgt agcacttgcc ttctcgcggt acccgaaccc gaaataaaat tttagtttgg
    16501 cgataaaatc ggtatccgcc atcgatactt catatttttt cgtatcagat tcatcggaaa
    16561 cttccacatt gacgaaccaa taagctttgg catgcttagg tcgttttaaa ccacaccagg
    16621 gcgactaatc gccctggtgt tcactttttt ttattttact tgaactcatt gactgttttc
    16681 cctttatttt aaacattata aagaccgatg agtgcttgcc gcgtcttttg tttttatcac
    16741 ggttttttgt gcaaaaaaac ctgccgatag ttggcaggtc gtctacgtgt taagctttgg
    16801 tgtggtcttt agctggcaat tggttttcta aatcagttcg gacaacaagc acatcgcaag
    16861 ctgcgtttct aataacatat tcagaaacag aaccaataaa taaacgttct acagcattca
    16921 atcctgtggc tcctaacata atcaaatcta cttgattatc ttctgggatt tctctggcaa
    16981 taatttgttt tggtgagcca tattctacga cagaggttac attattcaag ccagctttct
    17041 tcgcattgct ttcataatct gccaatgttt gctttgccat ttcagtagct tgttctgcaa
    17101 gcattccatc aaaggatgaa acggtttgga aagaacgtgt gtcaatgaca tgtgctaata
    17161 gaagttctcc gttatttcgg atagctacat tgaccgcctt tttaaaagct aattcagctt
    17221 catcagaacc atctacggca accatgattt ttttgtattg ttgtaacata agcattcact
    17281 cctcaagtca atcgtattgt actcttttat tgtagaacac tttagggaaa aagagaatta
    17341 tttactgctt tggttcagca attttttttg caaatcggat actgaaggtg gcagaaacaa
    17401 taattacgat cagtaaaaag accaaggcaa taaagcgttg attgaaaaaa gctgttaaaa
    17461 tacccattac gcctaaaaca gcatatgcag ctccgtaaaa ttgtaggaat ccttgatttt
    17521 tatcattttt gttgatcaat acaaaaaaga tatcctgttt attaaataaa taatagctaa
    17581 cgaataataa tactaaagtg gcgataatca ttaaaatcct aatcatgaac aaatcctcca
    17641 aaataagcaa caaatagaaa aagcaatttg ccaagagcaa attgctttat tctttaaaag
    17701 atggtaatct ccgttaatgc cgttgtttgt cctgtagtat tgatcccgcg aattcaagca
    17761 ggtgggtccc atggtcgtag cttcgaacta ccaaatgata aggcatgtta ccagcagtga
    17821 cacaagacgg gtcccaagat atcaataaaa atgttcggtc aacacacaaa gaggacaatt
    17881 cgaacatcac agatttccca cctctatagt agcatatgaa tttaaaaaga gcaatgagag
    17941 agctcgcaga aacagaaccg ttttcttgtc tagttttttg gaggatgctt tttccactct
    18001 tttatacggt aatactgttg acccagagcc tgttcatatt tccctgtatc ttttggctga
    18061 taatactgag cattcttcag tttatctggt aaatattgct gattgaccca agcctgatca
    18121 aaatgatgag gatactgata acccacgcca cggttgagtg atttcgcacc tttgtaatgg
    18181 ctatctctca aatgatcagg aacatctcca gctttgccct ctcgaatatc agcaagtgct
    18241 gcatctaatg ccatgtaggc agaattggat tttggcgaca agcaaagatc gacgacgacg
    18301 tctgctagtg ggatacgtgc ctcaggcaat cccaattttt cagcagctaa tacagcgttc
    18361 actgtacgag ccgcagcagc aggattacct aaacctatat cttcataacc gataaccatc
    18421 agacgccggc aaatacttgc cagatcacct gcctcaacta aacgggcgag ataatgaagt
    18481 gcagcatcca catcacttcc acgaatcgat ttttgaaaag ccgaaataac gtcataatga
    18541 gcatctccat ttttgtcatg ggtaagagct tttctttgga cacattcttc aatgatcgac
    18601 agtgtaagat gaatccttcc ttctttatct gaaggagtcg atagtgtagc cagttccagc
    18661 ccattcaatg cactccgcaa atcgccgttc gttgcacgcg aaaggtgaag aagagcatct
    18721 tcgtctaact ggattgcttg ttgtccaagg ccgcgttctt tatcccttaa agcatgttcg
    18781 actgccaact ggatgtcttg ttctgttaat ggtttgactt caaagatctg tgtacggctg
    18841 cggatcgctg gattgatggt aatataagga ttctcagtag tcgctccgat caagatgatt
    18901 cggccacttt ctaaatgagg aagcagaaaa tcctgttttg ttttatctaa tcggtgtact
    18961 tcatccaata agaggatcac tgtaccactc atttttgctt cttccgctac aacttgtaga
    19021 tccttttttg aatctgtagc ggcgtttagc atgcgaaatg cataattagt agagccggcg
    19081 atcgcacttg cgatactcgt ttttccagta ccaggaggtc catatagaat catggaagag
    19141 agcatgcgag catcgaccat gcggcgtatg atttttcctt ccccgactag atgctgctga
    19201 ccaacgactt catcgatcgt tcgaggtctc atgcgataag ccaatggctg ttgcatagga
    19261 ttttcctcct tcttgttttt acgtccctta attatatcat atttagcaag atgcctagaa
    19321 agaacagcct tctgttgttt tgttttcata gcttatccaa ctaatattgt gtatgataaa
    19381 gtaggtgaga aaaatgacgg ataaacaaaa gatagatgaa tttttttgga ataaaacgac
    19441 gccagaagtg gcacgggatt tattggggat gtatttggag catgacacac cagaaggaag
    19501 attagctgga tatattgtag acgcggaagc atacctaggt ccagaagacg aggccgccca
    19561 tagctatggt ttaagaagaa caccgcgtgt gcgggcaatg tatgaaaaac caggtacgat
    19621 ctacctttac acgatgcata ctcatcggat tttgaacatc attactcagc cagaagggat
    19681 tcctcaagga gtaatgatac gtgcaatcga acccgccgca atgattgatc aaatgagtaa
    19741 aaatcgtggc ggaaaaactg gtccagatat cagcaacggt ccgggaaaat tagtggaagc
    19801 attagcaatt cctcaagaac tttatggtca gtctatagca gatagttcgt tacgcttagt
    19861 ttttgagaaa aaaaagacac caaaaaagat caatgctttg cctagaatcg gtatcccaaa
    19921 caaaggtgta tggacagaaa agccgttgcg ttttgttgtg tcgggaaatc cttatatctc
    19981 tttacaaaga aaaaaccaaa tcgagaaaga ttggggttgg aggaaagaaa atgaaaaaga
    20041 aggatctatt aacatattta gatgaaataa ttgaaaaaca agtgacagac tatgatgtag
    20101 ctttagattg ggatacaaaa aatcatacga tcgagatcgt tgttcgcttg tttgcagaaa
    20161 ataaagcaca atttgagatt gatgatgcag aagggatcgt ttcgcaagaa ccaattatcg
    20221 aatttgaaga cggcgtactt ctttttaatc ctcagaaatc tgtttttgat gaacgcaatt
    20281 atctagcagt aatcccttat gaaggaaaaa aagggttggc aaaatcagta gctgattctt
    20341 tagtggaata tctaaatgaa gtattagctc aagggcaaag cgatttgctt gattttttgg
    20401 atgaagacga tgacgaagct gtattcgagc ttcactggga taatcagcaa ctagcagaat
    20461 tagtagaagt aaaacagcaa aatgaagcag atacttattt accgtaccca agttattaag
    20521 gagtgacaga tatgaaatgg aatgaagtaa aagtagaaac tgaaagtgaa gcagtcgaag
    20581 cagttgctaa tattttgatg gaggcagggg cgagtggtgt cgcgattgaa gattctttag
    20641 atgttgaaca ttttcagtcg gatcaatatg gtgagatttt agataaaaat gattttacat
    20701 cgctgaaaga aggcgcacta gtcatggctt attttccgga aacgactttc ttaccggaaa
    20761 tcttgccttt tatcaaagat cgtatcacac aattacctga gtttggtcta gctattggaa
    20821 aaaataaagt aacagttagt gaagtagagg aaagcaactg ggcaacagcc tggaagaaat
    20881 actatcatcc ggtgcgtatt acgcgaatgc tgacggttgc gccaagctgg gaaaactacc
    20941 agacagaaga tcctctagaa aaaattatca cactagatcc tggtatggca tttggcacag
    21001 gcactcatcc cacgacttcc ttgacattga cagcactaga aacagtcttg cgtggtggtg
    21061 aaacattgct agatgtggga actgggtcgg gcgtgctaag tattgcagca aaatatcttg
    21121 gtgcaaaaga tgtctatgcc tttgatttag acgaagtagc cgttcgttcg gcgaaagaaa
    21181 atatggatat gaatgaaata gccaaagatg tccatgtatc agctaacgat cttttgacag
    21241 gaatcgagat tgaaagtgat gtaatcgttg cgaacatttt agcagatatc atcttattga
    21301 tgatcccaga tgcttggcgt ttactgaagc aaacaggcac gctgatcgtt tcaggaatca
    21361 ttgaagagaa gaagcaaatg gttctggatg ccatgttaga acaagggttt atcgtggatc
    21421 aaattttcca gcaaaaagac tggtatgcaa ttattttgaa aaaaccagag gtggaataga
    21481 tgcagcggta ttttttaaaa gaatcatatg aatcaaaaga ctattttgtt ttagaagggg
    21541 aaaactatca ccacattgta cgggttatga ggatggaacc cgaggatgaa gtttttttgg
    21601 cattcaatga tcaaatagcc attgttgcag aaatcacgga gattactgaa gaggctgttt
    21661 atctgaaaga aattaaaaaa gaagaaaatc aaaaggaact gccaagttcg attaccatag
    21721 cgagtggcta tcctaaaggg gaaaagctgg attggatcgt tcagaaatcc actgaattag
    21781 gagcgcaagc ttttcttggt ttcccggcat cagcctctgt cgttaaatgg gatgcaaaaa
    21841 aacgtatgaa acgacaacaa cggcttgaaa agattgctca agaggcagcg gaacaatccc
    21901 atcggcagat cgtgccatca gtagagttat tggaaacgaa acaagattta tttgagcggc
    21961 tagatgatta tgacgtggtc ttggttgctt atgaggaatc agcaaagcaa ggcgaaaaat
    22021 caaatgcagc caaaattctt agtgaaacaa aaccagatag tcgaatctta gctgtctttg
    22081 gaccagaagg tggattatcc cgcgatgaag tcgattattt tgtagaacac aaagggatac
    22141 tttgtggatt aggaccaaga atccttcgta cagaaacagc acctctttat ttattaagtg
    22201 ctgctagcta tcaatgggaa ttaatgaatc cttctttcag ctaacgttct tcattaataa
    22261 aagagtatcg acagaaagaa ttgaaaaaat agtcaacaat ttgtataata gaaacaatta
    22321 tttatacaat ggatacgaaa aatcgtttga aaaagctttt aaaaagcgaa catttccgta
    22381 aaaaaacgta taactgcacg gattttggga caattatcaa acagatattt gtcccgattc
    22441 ccgttttacc gaaaaaagaa agggggatcg attcctgtga caaaagaaga aattttaacg
    22501 ggacccggtg tcataaagct cgttagtcaa tacatgggtc ccgaacatgt agcatttgtt
    22561 gagaaagcat gcgaatacgc gactgctgca catgacggac agtttagaaa atcaggcgaa
    22621 ccctacatta ttcatcctat ccaagtcgca ggtatattag cagatttaaa aatggatccc
    22681 catacagtgg ctacaggctt cttacatgat gttgttgaag atacagaaat cactttagaa
    22741 gatctgagag aagaatttgg cgatgacgtt gctatgttag tggacggcgt aaccaaatta
    22801 gggaaaataa aatataaatc ccacgaagag cagctagcag aaaaccaccg aaagatgctg
    22861 ctagcaatgg ctcaagattt acgagttatc atggtcaaat tagctgatag actacataac
    22921 atgcgtacgt tgaagcacct gcgagaagat aagcaaagaa ggatcgctca ggaaacttta
    22981 gaaatctatg cgcctcttgc acatcgtcta gggatcagcc ggatcaaatg ggaattagaa
    23041 gatacagcac ttcgttatct aaatccaaaa cagtattacc gtatcgtcca cttgatgcag
    23101 acgaagagag aagaacgtga aaaatacgta agtggtactg ttgaagatat tcgaatagcg
    23161 acagaagagc tggggatttt tgcagaaatc tatggacggc caaaacacat ttattcaatc
    23221 tatcgtaaaa tgaaagatca gaaaaagcag ttcaacgaaa tttatgactt gctagctatt
    23281 cgagtgatcg tagattcaat caaggattgt tatgctgtat taggagcaat ccacacaaaa
    23341 tggaaaccaa tgcccggcag atttaaggat tatattgcca tgccaaaagc taatatgtac
    23401 caatctttgc atactaccgt gatcggtccg gcaggaaatc cggtagaaat tcaaatccga
    23461 acacaggaaa tgcatgaaat cgctgaattc ggggttgctg cacactgggc ctataaagaa
    23521 ggaaaaaatg aaaaagtaga accagatggt atgacgaaac aattaagctg gttccatgag
    23581 atactcgaac ttcaagacga aagctatgat gcttctgaat ttatggaagg cgtaaaagga
    23641 gatatcttta gtgataaagt ctacgtcttc acaccaaaag gagacgttac tgagttacca
    23701 aaaggatccg gaccattgga ctttgcatac agtatccata cagatatcgg taacaaaacc
    23761 actggtgcaa aagtaaatgg caaaatggtg cagcttgatt acaaattgaa aaacggagat
    23821 atcattgaga tcatgacttc tccaaattca tttggcccaa gtcgcgactg gttgaaatta
    23881 gttgctacta gcaaagcaag aaataagatc aaacgtttct tcaaagccca agatcgagaa
    23941 gaaaatgtga tcaaaggcca cgaatccgtg gtcaaatgta ttacagatct aggatttacg
    24001 cctaaagata ttttgacgaa gaacaaactg caagaagcac tcgatcgttt taattatcaa
    24061 acagaagatg atctctatgc agctgtaggg tatggagaag ttagcccctt gacgatggcc
    24121 aatcgtctga ctgaaaaaga acgtaaagaa caaaaaatcg agcagcaaaa gcaagaagca
    24181 gaagaaatca tgaatcagcc gaaaaaagaa cctgacaaaa tgaaagtacg tcatgaaggt
    24241 ggcgttgtca ttcaaggggt agaaaactta ctaattcgta tcagtcgctg ctgtaatccg
    24301 attcctggtg atgatatcgt tggttatatc actaaaggca gagggatatc cattcatcgt
    24361 cgagattgtc cgaatgttca gcctgacaaa ccaaatgtag cagaacgttt gattgaagtc
    24421 gaatgggaag atacatcgaa tacacgaaaa gagtatgatg cagatttgga aatttacggc
    24481 tataatcgtt caggcttatt gaatgatgta cttcaaacag tcaatgcgct aacgaaaaat
    24541 ctcaacagcg ttgaagcacg gacgaataaa gataaaatgg cgacgatcca tttgacggtt
    24601 ggtatccaga atttatccca cctaaagagt atcgtggata aaatcaaagc agtacctgat
    24661 gtctacagtg tacgccggac gaatggatag gaggacagat atgagagcag ttgttcaacg
    24721 ggtaagtaaa gctgctgtgt cagtcgatca gcaagaaatt ggaaaaattg gctacggcct
    24781 acttgtttta ttaggcattc atgaaaccga tacgcaaaaa gatgtggact atctggtaaa
    24841 aaaaattgca caaatgcgta tttttgaaga tgaacaagga aaaatgaata ttagtgtaga
    24901 agatgtggga ggacaaattt taagtatctc tcaattcaca ctgtttgcgg atacgaaaaa
    24961 aggaaatcga ccaagctttg tggaagccgc acgaccagaa acagctattc ctttatacga
    25021 atcattcaat gaaggaatca gaagtcgcgg catcacagtc gctaccggac aattcggagc
    25081 agatatgtcg gtttctctcg tcaatgatgg acctgtaacg atcattattg attctaaaaa
    25141 caaataaagt aatctggttt tcactagtaa aaactatgaa acacgtcgct taagttagac
    25201 tgataaactc taacttaagt ggcttttttg tctgcaaaaa gcgcttcttt tgtcagcagt
    25261 cggaaaaaaa gttaagctta aattatacaa gttatttttt ataggaggtt gctgaagatg
    25321 aaagaagtac cagtagtaga tatcaaaatc acagatttaa aaaaattgta tcaaaaagga
    25381 tacaacatgt tggaggagtt acgccatgaa gcggacgctc ccgtcgtcaa agcaaagatt
    25441 ttcaataaag aagctatcac gatctacgga tcatcagcag caaaagtatt ttatgatcca
    25501 aggaatttca aacgaaaagg tgctatgcca aaattagtat tgaagacact ttttggtcaa
    25561 ggtggagtac agactttgga tggagctgct catcaccatc ggaaaaatat ttttatggat
    25621 ctaatgacac cggaaagaat ggaagattat catcgtatct tagataagaa tctgacacaa
    25681 gcacttgaag cacagcatgg tcaatttgaa ctatttgatt taagtaagat ggttttcttt
    25741 acatctattt gcgagtgggc aggtatcaat ttatcagcga tttctaaaga tgaggtcgaa
    25801 aaattagcag aatatcaaat ctctatgatc agcggaacgt ttacttctcc gattgatcac
    25861 ataaaaggtg tcgaaaatcg caaaaaatca gaaaaatggg cgcaaggctt gattgaagaa
    25921 gctcgacaaa atcctgtggc tggaaaagaa aatgtggcat tatatgcttt tgccaatgcc
    25981 acagatttag atggtcagct tttacctctg gaagtggcag ctgttgaatt attgaacatc
    26041 atccgtccaa cggttgctct aactgtgtgg gcggcattga tgggacatgc acttttcagt
    26101 agaccagatt tgtatcagca attaaaaaat gacttttcga cgttacaaga tccgtttatt
    26161 caagaaatga gacgctatta tcctttcttc ccaatgctac ctgcgatttc gctgaaagaa
    26221 gtagaagtag atggttatcg tataccagaa ggcagttggg ttattttaga tttatatgga
    26281 acagaccatg atgaacgaac agttgaagcg ccagattcct ttatgatcaa acggtatgta
    26341 gggaaagcaa aagatatttc ctataaagaa gaatatgaga tgattgcaca aggcggggga
    26401 aattttcgac aaatgcatcg ctgtgcagga gaatggatca ctcttcatag tctacgtgtg
    26461 ttttccgatc agctagtaaa taagtttgag ttctctgtac ctgaacaaga ttggacgatt
    26521 ccattcaatc agtttccgac ttatccgaat agccgagcgc tactttacaa gaattagatt
    26581 agcataaact tatcgaaaaa aaggaagatc aaacagtgct gatcttcctt ttttcatatt
    26641 actataaatt gataaggtca attgatcaaa atccacgaaa tgcttcatca atggtttgat
    26701 aatcttcatt ggataaatgc aaatctaaag cttggacatt gtcagatact tgctctggac
    26761 gcttagctcc aggtataact acagacactc gtggattctt catgtaccaa gctaaaacaa
    26821 gttgcgcagg agtggcttgg tatcttttag caatggggcg aagtacatcg acagcggtta
    26881 aaatttctct gaaacgttct ccttggaaat ctggatttct ttttcgagga tctccttcac
    26941 caaattgttt ttcctcacca agttcatatt ttcctgtcag caaaccggaa gcaagaggaa
    27001 aatagggaac aaaactgatt ttgttttttt caagataagg gaataattct ttttcagcct
    27061 gccgatggat caaactgtat ttatcttcta ctacatcgac atatccatct gcattcgctt
    27121 ctttcaattg ttctaacgtg aagtttgaca caccgactgc tcggattttt cctgcttctt
    27181 ttaattcgtg caatgtagca actgattcat tttttggtgt gctttcatcc ggaaagtgga
    27241 tataaaaaat gtcgatgtaa tcagtttgca gacgttttag tgcatcttcc accgattgtt
    27301 tgagaaactc aggagaatta tcaaatgttc gccctttatt tggtacatga gcagcttttg
    27361 tagcgatgac gacacgtgag cgatcatatt ctttttcctt cagtacctcg ccaatcagtt
    27421 cttcggaacg gccgtttccg taagcataag cggtatcgat caaattgatc ccgctattca
    27481 aagctgtacg aactacttgt tttcctgtct catcttccaa accgtcaaaa agattatgtc
    27541 ctccaacggc atttgctcct aatccaagct gttctgcgta gacttgtgaa tgtccgattc
    27601 ttatttctgc catagctgat tcctcctttt tgtctagtct aaaacaaaat accaaaaagc
    27661 tgaactaaaa cgtttttgtg aggggaaata gtcagatatc tcttgacttt gagactggtt
    27721 tttagtaggt tataagtaat atcgaatgtc aatgaaagag cgagtaagtt tttgaaactt
    27781 tacaagagag gatcatcgag gctgaaaatg atctaaagtt tcattagcct aaagacactc
    27841 tggagatgga cgagtgaaaa gccgtcccgc gtacgagcgt tattcgtaag aggaaagaga
    27901 aatctttcaa aattaggtgg taccgtgtat gaatttcatt cacccttgtt catgatgaac
    27961 aagggtgatt tttttattta catagctaag aaagggagag aaaactatga gtttccaaag
    28021 accaaaagga acaaatgata ttttgccagg cgaatcagaa aaatggcagt ttgtcgaaga
    28081 aacagcacgt ttattattca gagattatca gtataatgaa atgcgtacac cgatttttga
    28141 acactatgaa gtgatatccc gaagtgtagg ggatacaacg gatatcgttt caaaagagat
    28201 gtatgatttt tatgacaaag gggaacgcca tgtgacgctt cgaccagaag gtacagcacc
    28261 aatcgttcgt tcttttgttg agcataaatt gttcggccca gagtatgcga aaccgttcaa
    28321 aactttttat atgggaccaa tgtttcgcta cgaacgccct caaaaaggac ggttacgtca
    28381 attccatcaa atcggagtag aagcatttgg atcagaaaac cctgcaactg atgtggagag
    28441 catggtgatg gcacttgatt tcttcaaaca gttggggatc acgcaaatcc ggctagtcat
    28501 caattcatta ggtgataaag aaacgcgtca agcctatcgt caagcattga tcgactatct
    28561 tttacctttt gaatcagagc tgagtgaaga ctctaaacgc cgccttcatg aaaatccatt
    28621 acgcgtattg gacagtaaag acaaacgaga tcaaaaattt gtggcagaag ctccatctat
    28681 cttagattat ctaagcgagc ctgctcaaaa acattttgat accgttgttt ccatgctgga
    28741 tgcattggct gttccttatg aaatcgacag taatatggtt cgcggactag attattacac
    28801 acatacgatt tttgaaatca tgagtgacgc accaaaaatg ggggcacaag caacgatttg
    28861 cgcaggcgga cgttatgaca atctagtgga agaactaggt ggtccttcaa cacctggctt
    28921 cggatttgca atgggtatcg agcgattact cttgactatg gaggcagagg aagtagttat
    28981 ccctgcattt aatgagttgg atgcttacgt ggtcgcactc ggagacgaaa caaatatcga
    29041 agcattgaaa gtggtccaag cgattcgaaa ctttggtttt tcagctgacc gcgacttcat
    29101 gaaccgtaaa gcaaaagcgc aatttaagac agcagataaa gcgaatgcca aattagtatt
    29161 ggtgatcggt gaagatgagc ttgctagtca aacgattaat gtcaaagcaa tggcaagccg
    29221 aaaagaaaaa agttttccat tagaagagat atatgaacat tttgatgaag tctatgatga
    29281 aatgacttta ttcgagtagg aggaaaaaaa taaatggcac aaagaacgat ctattgcgga
    29341 ttagtttccg aacaacaatt agaacaaaca gttacattaa aaggatgggt acaaaaaaga
    29401 cgagaccttg ggggagtaat ctttatcgac ctacgtgatc gtgaaggaat cgttcaaatc
    29461 gtctttaatc ctgaaatcaa caaagaagca tgggaaatcg cggataaatg tcgcagtgaa
    29521 tacgtgatcg aagtgactgg ggaagttaga agacgcggag aaaatgcagt aaatcctaaa
    29581 atggccactg gtgaattcga agtgattgca agtgaaatcg tgattttgaa tcaagcaaaa
    29641 accccgccat ttcaaattga agatgatcaa gcaatcagtg acgaaatacg tttgaaatat
    29701 cgttatttgg atctgcgtcg tccaaaaatg actaaaaaca tcaaattaag aaatgcgaca
    29761 acaaaagcga ttcgtcatta tttagatgat aatgactttc tcgatatcga aacaccttac
    29821 ctagggaaat ctacaccaga aggcgcaaga gactacctag ttccttcacg tgtccatgct
    29881 ggacatttct atgcgttgcc gcaatctcct caattgttta aacaattgct gatgaatgct
    29941 ggattcgacc gttattatca aatcgttcgt tgtttccgcg atgaagattt acgtggagac
    30001 cgtcagcctg aatttacgca agtagatatt gaaacaacgt tcttaacagc agaagaaatc
    30061 caaacttaca cagagggatt gattgcgaag gtaatgaaag aagttcgcgg tatcgaagtc
    30121 acattgccat ttcctcgtat gacctatgat gaagcaatgg ctcgctacgg cagcgacaaa
    30181 ccggatacac gttttgatat ggaattgatc gatctaagcg atacagtcaa agaagtagaa
    30241 ttcaaagtct tccaaatggc tcttgaaaac ggcggtgtcg tgaaagcatt gaatgctaaa
    30301 ggcgctgcag atcgctactc acgaaaagac atggatcagt taggtcaata tgtaggacaa
    30361 ttcggagcta aggggcttgc atggctgaaa gtagaagaag acggattgaa aggtccaatt
    30421 gctaaattca tgggagaagc aacagaagca atcattaaag caacagatgc aaaaccaggt
    30481 gacttgctga tgtttggcgc agataaaagt gaaatcgtcg ctgctgcatt aggtgctatc
    30541 cggacacgtt taggaaaaga attaggacta attgatgaat cgaaattcaa tttcttatgg
    30601 gtaactgatt ggccacaatt tgaattttca gaagaagaag gtcgatatgt ctctgctcac
    30661 catccattca cgatgccaaa agaagaagat atcccgttat tggctgaaga ccctgcaaaa
    30721 gtctatgcag aagcttatga tatcgtcttg aacggttatg aactaggcgg aggttctatc
    30781 cgtattcata ctcgtgagct tcaagaaaaa gtactagaaa ctctaggctt tacaaaagaa
    30841 caaatggaag aacaatttgg tttcttgtta acagctttag attacggatt ccctccacat
    30901 ggcggtattg cattaggatt ggaccgtttt gcaatgttat tagccggaga agataatatt
    30961 cgtgaagtca ttgcctttcc taaaaacgga aaagcagcag atgtgatgag tgatgcacca
    31021 agtatggttt cgcctttaca attgttcgaa ttgaatattg atgtaacgaa tatagaagaa
    31081 tagccgataa aaaggtaaaa aggatttgtc gaatacgctt cttttgacaa ccttgattta
    31141 attgaaaaac actttataat gaagccataa catataaagc ggggcttttc catgaaaaaa
    31201 acattcgaaa attgggctta tcatgattac acgaccaagc tttcggtatc tatcgtttat
    31261 gcgttgatcg catcagtcgc attgaatttc ttttatcagc caggacatat ttattcaagt
    31321 ggtattactg gtctagctca gattttgacg actttgtcaa aatcactgat aggattcgaa
    31381 gtaccggtat caacaacttt gtatttactg aatatacctt tgtttgtatt ggcgtggtta
    31441 aaaatcggga agaagtttac aattttcact tttatcacag tcactttgac ctccctttgt
    31501 atccatattt taccggaaac agtcctatct accgatccaa tcatctgtgc gttgtttggg
    31561 ggggctgtga tgggactagg tattgggcta gcgttgagaa acggcctatc atcgggcggt
    31621 ctagatatcg tgagtatcac gattcgtaaa aaaacaggaa agtcgattgg ttctatttcg
    31681 atctacttca atgcattgat cgtttttgtt gccggaattc tatttggctg gcaatatatg
    31741 ttttacagta cgttgtctat tttcgtcagt ggaaaagtaa ctgatgtcgt ttatactaaa
    31801 caaaagaaga tgcaggtaat gatcgtaacg aaacatccag aaaaagtagt aaatgaaatc
    31861 caaaactgta tgcgtagagg gatcacgatc atcaatgaag cagaaggtgc ttataagcat
    31921 gataagcaga cagtattgat gacgatcgtt acacgatacg aactgcctct acttagagaa
    31981 gcaatgaaag cttctgatcc acaagctttt gtaagtattg cagaaaacgt ccagatactt
    32041 ggtcgtttct atgaggatgg catttagatc gagtttgaca aaaattcatg aaatactgct
    32101 gtagacagac gttcgtttgt ctgcagtttt tttctaccat agtagaatca gcaaagaaaa
    32161 actgcttttt tatttgaaat tttcttaaag ttgaaaaaaa gcatgtttat ttttttaaat
    32221 ttatgggatg atagaaaaaa atagtagaga aaggacacga ttctacttgc tattttcact
    32281 taatacccgt atattacgaa tgaaaaaagt ttatgaaaag ttggaatatt gaatggaaaa
    32341 acaaaaaagg ttaaaaaaca gccgctacat cactgggttt gatgggctcc gttcattagc
    32401 tgtggtgggt gttatcttct atcaccttct gccaactagt atgaaaggcg gctatttggg
    32461 cgttccgatt tttttcgtcg tatctggtta tttgatcacg gatctgctgc ggcaggaatg
    32521 ggaacaaaat ggaaaaatca atatttggca attttatgtc agaagaatga agagactcta
    32581 tccggcactt gtttttatgc ttgtgacagc ttcggcttat atcactttgt ttcagcgagg
    32641 gctgttaaac aatcttcgag gagttgtggt cagcagctta ctgtatgtca acaactggtg
    32701 gcaaatcaat aacggtttat cttatttcga tcgatttgct aatgagtcac cgtttacgca
    32761 tatctggtct ttagcagtag aaggtcaaaa ctatttggtt tggccgatct tgtttgtatt
    32821 gttgatggta tttgttcgta aaagaaaatg gatcttttat actgtgttag gtacatcctt
    32881 cctctccgcc atcttgatga tgatcttgta tacaccagga ggcgatccaa cccgtgttta
    32941 ttacggtacc gacactcgtc tgttttctat atggttggga agtgcgcttg cgtttgtttg
    33001 gccaagtacg cgtttaaaga aaaacatccc gaaacaagcg aaaaatattc tgaatatcgc
    33061 tggaggaatc tcgcttttgg cactgattct ttgcttcttc tttttagatg atcattacaa
    33121 tttcatctat tacggaggaa tgctcctagt gagtctgtta tgcgtagtgc tagttgcagt
    33181 gacagcacac cccggagcaa gtttgaatcg atggctgaca aatccagtat ttacttggat
    33241 tggcaaacga agctatggca tttatctgta tcagtttcca gttatgattt tttatgaagc
    33301 aaaggtgact gatattgccg atcatgtttt gttgcatact ttagtggaaa tcacattgat
    33361 tctaatcatc agtgaacttt cttatcgata tgtggagcgt ccgctagcac gttttgatta
    33421 tggtcgtaca ttccaagtga tgaaaggatg gttcagcaaa ccgatcatca gtaagagcaa
    33481 accatgggtt gttccaggca cattacttgt agcaattgca ctcgttggtt tcgttacggc
    33541 accgaaaaac gctgtaacag ccgatcaaaa acaactgcag gcaaaaatcg ctgaaagtaa
    33601 aaaattagca gaagaaacca gaaacaattc agaaaacacc actagttcgg ttgaccaagc
    33661 gatccttgat aagtacgagt tgtccaaggc acaagggaat aaggcacaaa aattagaact
    33721 aacggcattt ggtgattcag ttatgttgga tgctgcaacg gatctacaag aactttatcc
    33781 aaaagtagtg gtagatggag atgtcggacg acaattatat gccagtgtac cgtacattga
    33841 aaaattaaag gaacaagatt tgttgaaaga cactgttctg atcggactag gaacgaatgg
    33901 ctcattctcg gaagctcagt ttgatagtgt gatggatgca atcggtgatc gaaaagtata
    33961 ctgggtcaat gtccgagtac cgacgaagcg ctggcaaaat gacgtgaatg cgatgcttga
    34021 acagatgacg aaaaaatacg acaatatgac gctgattgat tggtacagct acagtaatga
    34081 ccatagtgac tggttttatg atgatcaagt ccatccaaat cctgaaggga tgacacatta
    34141 cattcactta gtcagcgaag ccttgctagg aggaagttca agtacgaaca gcagtgagac
    34201 aaacgtgaca gaatgatagc tacaaaaaac ttcaggcaaa aacatttgtt tttgcctgaa
    34261 gtttttttat atttaccgtg tttttaacat tcatatagat tcaggaagca aaacggttga
    34321 gtgtgtctgt aaataacgtt ctttttgttt tcgggtgagt tttttgaatg cagcttctgc
    34381 ttttgttgct tcgcttcttg aagaaaatac ttctgcatgg atcatcttaa caggtcttct
    34441 tttttgaaga cgagtatatt tagcaccagt accattgtta tgttcctcta atcgtctaga
    34501 aagatcggtt gtatatccac cataaaatgt ctgatcttta cagaataata cgtaaaaata
    34561 atgttcattt gccatagagc atctcacgaa tctctgcagt gtattctcca tccatattgt
    34621 aagtgatcaa tggtggagcg actttgaatc cgtctaattt gccttctttg attccttcaa
    34681 ctaacaaaat atttgcctct tttccaggtt ttgggtaaac aaattggatc cttttaggtg
    34741 caattcgata gtcttccatg acctgaatga tttctaagaa ccgatcagga cgatggacca
    34801 tagtaaagcg tccatttgtt tttagtagct tacttgaaat atcgatcact tcttctaacg
    34861 tcgtatgaat ctcatgacgg gcgattgcca ggtgtttatt agggttcgtt ttatttgttg
    34921 gcagcccttt gaaataaggt ggattacaga cgactaagtc acaagaatta tgtcgaacga
    34981 cagataagct gtctttcaga tcgatggtgt ggacagtcat ttgttcattt aaatggttaa
    35041 gcgcgatact acgtcttgcc atgtctgcta gacgaggttg aagttcgata gcgtcgatag
    35101 ccgcctgggt tcgtttactt aaaaaaagtc caactgcgcc atttcctgca caaaggtcaa
    35161 caatcttccc ttttttaggt acacgggaaa agtgagcaag cagcacagca tctaaagaaa
    35221 aagaaaacac tttggaactt tggataatct tgatatcttc tgcgtataat tggtcgattc
    35281 gttcgtcttc gtgaagcatg gattccctcc aaaatgaaaa aaatagtcgt ctgtattata
    35341 ctatttttat taaaaagaac caaggtggct tgtcaaacct ctatttttta tgcatactat
    35401 gaccataaga agggaaagga agagactatg ttttatcgat ttatgcgcgg ggtagttcga
    35461 gtactattag ccttagttaa tggaaatgcc cgctatgaga ataaagaagt tttaccgcaa
    35521 gatgagaatt acatactagt tgctccccat cgtacttggt gggacccatt atatctggcg
    35581 gtagctgcag cacccaagga attcagcttt atggcaaaag aagaactgtt caaaaatcca
    35641 gttctgcgat ttatattaac tcgatccaat gcttttccag ttaaaagaga aaatccagga
    35701 ccaagcgtca ttaaaacacc tgtaaagatt ttgaaatcga caaatttgag tttgatcatg
    35761 tttcccagtg ggacaagaca tgctacagaa cttaaaggcg gaatggcatt gatttccaga
    35821 atgtctaaag cgaaaatcgt gccagctgta tatcaaggtc cgttgacact tggggatctg
    35881 ttcaagcgtc agagagtgac agtgcgtttc ggcgaaccaa tcgacttatc agatatcaag
    35941 aaaacggata aagaaggtat ggcggagatc gaacgacgaa cacaagcagc ttttgacaaa
    36001 ttggacaaag aagtgaaccc tgatttcaaa tatgaagtca aaaaaaaata aaatgataag
    36061 tttatgcgtg ggcggtcttt tgaatctact gtcctgttca gttcagactt tttcaatgaa
    36121 aacgagggag agacgtcaag tctgccctct ttttgcgttc caatatgtaa aatagaagac
    36181 aggtgtcatg taatggatca aagtgttata ataattagaa ggacattttt tgaggtgatt
    36241 gaatgcgttt tttacataca gccgattggc atatcggaaa aaaactacaa ggttatgact
    36301 tgattgaaga ccagcgtcat gtactatctt ctatactaga tatcgcgaaa gaagaaaaag
    36361 tagaggcaat cgtgattgcc ggagacttat atgataggag tgtcccagct gttgatgccg
    36421 tagaattata caatgaattg atggtcgatt ggaatttaaa ggaaaagatc ccgatttttg
    36481 ctatatcagg aaatcacgat agcagtacga gactttcagc gggtacgcca tggttcagtt
    36541 attcgaacta ttttttgcga acacagctag cagaagcatt tgaaccagtc gagtttaaaa
    36601 acgtacaatt tttctttctt ccttactttg aaccaattgc agcgagatta tattttgaag
    36661 atgacaatat ccgaacgatc aagcaagcgg tagccaaggt gatagataaa atgcaggagc
    36721 tatttgattc agaaaaaaaa caagtattga ttagccattt ttttgtaatg ggaagtgtaa
    36781 aatctgattc ggaaacgaaa atcgaagtag gcgggttgga ttcgatccct ggtgaactac
    36841 ttacaccgtt tgattatgta gcactaggac atttgcataa tcaagctgct ttgagacagg
    36901 ataatgctcg ctatagcggt tccccattga aattctcttt atctgagatc aaccaaaaaa
    36961 aaggcgtatg gatcgttgac ctgacaacag gcctggagtt gaattttagg gagatcgaac
    37021 ccctgcatga tattgaagaa ataacagcga gtttcaaaga attgctggat cctgcctttt
    37081 atcagtccgt caatcgggag aattacatct atatctatct cactgataga acgatcatcc
    37141 cgaatatgat gaaccagctt cggcaaatat atcctcgtat tttggggatt gaacgggcaa
    37201 acggtcgagc agaacaaaca aagcgcaaac gaaaagtact agaaatgaaa gatcctcaaa
    37261 aattagcaga agcatttttt agtgaaatga cggaagagca gctaactgca aaccaagaaa
    37321 aatggctcgg agaaacgttg caagaaatca tggaaatgga atgatcaaga atgaaaccga
    37381 aaaaattaca acttaaaaat tttggtcctt ttatcaatga gaccgtagat ttttcacgtt
    37441 tgacagaagc accgcttttt ttgatcagcg ggaagacagg agctggcaaa acgacgattt
    37501 ttgatgggat caccttcgca ctttttggtg aaacttccgg aagacttcgc tctggaaaag
    37561 agatgcgttc tttgtttgct acaccagacg aagaaacaag cgtgactttt tcttttgaac
    37621 atcagcagat gagctatgaa atcgaacgaa aaccagaaca agttttagca aaacgaaaag
    37681 gaaacggaac aaaaaaacag gcagccaaag taacgttgac catttttgat gggaaaggaa
    37741 aagaaataaa acaaatcaca aaacgtacag aagcagatca gttgatcaga gaactgatga
    37801 atctcgatgc gaaacagttt tctcaaatcg ttttactacc ccaaggagaa ttccgtaatt
    37861 ttttggtttc atcaagcagt gataaagaga ctgttttacg tcatttattc ggtactcaat
    37921 ttttccaaca gttcaatgag cgattaaaag aaaaagcaaa acaacagcaa caaaaattgg
    37981 atcatttaga gcaagagctg catcttctgc aaaaaaggtt tgttttagtc aaagaagagg
    38041 aaccagcagc agggtttgaa gccgttttag cccaatggac aaaccatcag gcaatactcg
    38101 aaaaacaaat cacggaagaa aaagaagctc taacggaact tcaggaaaaa caaaagcaac
    38161 tagaaaattc ctactatcgt tttgaaagct tgaaaaaaag ttatgaagaa aaaacgatat
    38221 taataaacaa gcaaaaggaa ttagctgctc aaaaagacga aatagaggaa aagaaacgat
    38281 ggattgggta ttatgaattt acgcaacgat tagtcgaacc aatccagcgc ctacaagaaa
    38341 taaaagttga aaaaatagaa ctgtcacaaa aaagtagtga gcaaacaaag aaactagcga
    38401 tgactacgca gaattatgaa gagtggcaaa aaaatgaagc aaaccgcgta cgccgacaaa
    38461 caaaaatcaa agaagctgct caaaaactgc aaaacaaaca gatgatggtg ccaattgtcg
    38521 aatccttacg tagtaaaaaa caagacgcag aacaattaac aatttcattg gataaagaag
    38581 aattgaatgc cgagaagctg tatcaaaatc tcgctatcta tcaggaaaga actcgaattc
    38641 ttcaagaaaa tttactggat caggaaaaac tgcaagaaga tcgtcttgct tttggacagt
    38701 taaaatatct agatgaaaag cagcaagaaa tagaaaaaca aaaagcaaaa gacactgggt
    38761 cgctcgcaca gataagagaa gaactcaaaa aagcaactga agaaataaca aaaaatagtc
    38821 agctattgaa agaacagaca gaacaagcag aacagctgaa aagccaatgg gcaaagcagc
    38881 aaattgctcg tcttcagctt tttttgcgtc caggggagcc atgtcttgtt tgtggatcaa
    38941 cggaacaccc catacaacaa ttaaaacacg aacagccaaa taaggaagat atcttaaaag
    39001 tagaacaaca actggcagaa gctgagtctg ctgtagaaag aactcagcaa atcattgcca
    39061 aaagtgaagc acatgaaaat aatcttcaac aacaattatc agatttagaa caaaaaatca
    39121 tggcaacgaa tatacaattg gctgaacaag aagaacactt gcaacaggct ttgaaaaagg
    39181 catttcctga gataacagaa caaaaaacaa aaacaactct tgtagtaatc gaacaatctt
    39241 tagatagacg aaaaagcaag atggccaatg caagaataga gctggaagaa ataagacaac
    39301 aagcagaaaa gctcgaacaa caattagctg tctacaaaca gcagcagaca gaaaaaaata
    39361 atcagctcca gcaactgcaa ggagaaatca aaagtttaga acaacaattg caagatacgg
    39421 atagtaactg tttagaagag gagatacgac gacttgaaaa acagttagaa gaagactcga
    39481 atcaactagc tgttgagcag aaagcaggag tgcaattgtt gcaagatctt catacgcttc
    39541 ggacacagct tgatcttcaa catgaacaaa tagaaaaaat agatgaaaaa atagaaaagg
    39601 aacaagaaag aatagctaaa aagttagaaa gccagtcgta tttttcaaca attgaagaaa
    39661 tctgtgcatt tgcacaacag caagcacaat ataaagaaaa ccaacgtaca gttacttctt
    39721 atcatgaaga gcaattagtg acagctgacc ggctgcgtca attagaaaat atccagctaa
    39781 ctgagacatt cccagataca gaagagatca gccaacagtt agaagaaata agacgtatga
    39841 tcgaaataaa gcaagaaaag atatacggtt tacaagaaca aaaaattagt aatcaaaaaa
    39901 tctacgatga atgtctttcg atttatcaca caagccagac gaaattagat gagcttacgc
    39961 aattacagca gctttcgcaa acattcaacg gagaaaatcc taagaagact agtttggaac
    40021 ggtatgtgct tcaggtttat cttcaagaag tgttacaagt agcaaatgat catctccaac
    40081 gtttaacgaa aaatcgttat caatttgagc tagcggatac cattggcagt tatcgaggga
    40141 aaaccggatt agaaatcaat atctatgatg atcaggcagg tatgtctcga agtgcgcata
    40201 ccctgtctgg cggtgagagt tttattgcag cactttcttt agcattagct cttgccgatg
    40261 tgatccagac acaagctggt ggtgtagcaa tcgatgcatt gtttattgat gaaggatttg
    40321 ggtcattaga tgaagaagct ttggaaatgg caatggaagc tttagaaacg atcgaaagtg
    40381 aaggaagaat gatcggcatc attagtcatg taagagaatt gaaagaacga gtattacaac
    40441 aaataagaat tgatacagaa ggtagcggac aaagcaaaat acgctatcaa cttggataaa
    40501 aagggagatt ggtaactatg aaatggagta ttccagaaaa agtcattgca cgtggacgtc
    40561 aatacttgca agaaggccga gtgttatcgg ttgtccctga ccaagaagaa aacgtgtggc
    40621 atgcagaagt cttgggcagt gaattgtata tggttgattt ggatggtaca gcaaaagaga
    40681 tagattactg ccaatgtccc tattgggagg aacataaata ttgtaagcat acagttgctg
    40741 tggaacttta tttacggtcg aaaaatctat ctcgtatcat gaaaaaagac cagccaatgc
    40801 tagaaccggt aaaagcaaca agtgagggag aaatcattgc aaaaggtttt tctcgtctaa
    40861 aaacacaaaa caatcctcaa gcggtgcaac ctttgattat tgaataccaa gtagaaacga
    40921 ttgagactaa tcagtatcac ccagagcttt ctattttggc actttacttg cgtatcggtt
    40981 cattggcatc accgaaaaaa acatatatcg taaaaaacat ctatgatttt ttacaagctt
    41041 acacagaaaa agaaacttac actgttaata aacagtacca gtttcgtcta gataaacggg
    41101 cgtttcaaaa agaagatcaa aaaatactca cgcatcttgc aggaagtgct cagacgcatc
    41161 aattattagg tacgaacggc ttacaagtca aaggaaaact agataaaaga tatctgcttc
    41221 tcccagtaga acaagcccgc ttcctgttag agcagatgaa tcagactaca cgtttgcatc
    41281 tgcagataaa tgaacaaaac gatcaaggaa tacaattttc cgatcaaaac aaacctttat
    41341 cattcaaagt ggaaaaattt ggagaagatt atttgctgca agcattgaat gattttgatt
    41401 ttttctttat gcattatcag tggggagtta tccaagggac attttattct ctgacgaaat
    41461 atcaacaaac ggtttatcaa acttggaaac aattgattcg ccgattggag aaaccagaag
    41521 tagtgttcaa gaaaaaagaa ttaccgtctt tgtttaaaga gattatccct ctgctgtcag
    41581 agatcggcga cgtagctatt gcccctgcag tagaagagga agtagcagat atccccgtcg
    41641 aatttatttt ctttttccga aaagcaaaag gaaaaatcca agctcgagtc gatttcgtct
    41701 atggcgaagt catctattcg actgacccga agcatgaggt ctcgacagaa caggcttttc
    41761 aggtgttacg tgacacagta gaagagaaac gagtacttga tctattcaga atgtatcgct
    41821 accagaaaaa tgaaactggt tatgaacgtc cattgccaag cggggaagaa ctatatgcct
    41881 ttttccgcac agaactatct gtcttccgtc agtttggtga agtcagactt ggaagaaaat
    41941 tgcgagagtt gtatttagat gcgcataaac atcaaccgaa aatcgaagta accgagaccg
    42001 gttcatggct cgatattcga ttcgatgtga cagggataga ggagcaagaa atcgatcatg
    42061 tcctgcaaag cttgctgaga aatgatgctt tttatacatt agaaaatggt caggttcttt
    42121 cttttgattc agaagaattt caacaaacaa gccaagtgtt gcaacaatta cgggaatcga
    42181 tacgtacaga agaaggaacg atccatgtac ctaaaaatca aggattgatc attcaaaatc
    42241 aattggaaaa aagcaatgct acttttagtg aatcgttcca aacgatggta caagatctca
    42301 ttcatccaga aagataccag gcacagcttc ccaaaggatt gaatgcaacg atgagagatt
    42361 atcaaaagca aggttttcga tggttgaaga tgttaggtca ttatcagttt ggcggtattt
    42421 tagccgatga aatgggactt gggaaaacac tacagacaat tgccttttta ctttcggaaa
    42481 aagaagaacg gaaaagcttc tctgctttga tcgtagcgcc tgcgagctta atttacaact
    42541 ggcaggcaga ggtgagaaaa ttcgcccctt ctttaagtat ccaagtaatc aatgggaata
    42601 aaaaagaacg tgaagaatta ctggctaaag acacagatat ccgagtgact tcttatgcca
    42661 gcttaagaca ggatctagct aattatcagt cgcaaaaaat tgattatttg attcttgatg
    42721 aggcacaaat ggtcaaaaat agcagtacga aaactgcaca agcattgaga gaattagctg
    42781 tacctcagcg gtttgcactg agtgggacac caatcgagaa taatttagaa gaattgtggt
    42841 cattgtttgc aacgattatg ccaggatttt tcccgactaa aacaaaattc agggaacttg
    42901 ccgcagaaga aattgcgcaa atgattcgac cgtttatttt acggcgagat aaacaaactg
    42961 tgttgaaaga cttgccggaa aaaacagaga tgaatctata ttctgcactt acagaagaac
    43021 aaaaaactgt ttatttagct tacttgcgtc aaatgcgtga agaaatcaca tcaatggatt
    43081 ctgaagcatt caagaaaaac cggatcggga tacttgctgg tctgacaaga ctgcgacaaa
    43141 tttgctgcga tcctcgtctg tttattgaag attaccaagg cggttccggc aagctcgaac
    43201 aagtaaaaga tctactggta gctgccaaag agaacaaacg aagagttttg ctgttttctc
    43261 aatttacagg gatgctgact atcttgcaag aagagttagc cgagctaggg atctcgacat
    43321 tttatttaag aggcagtacg aaaccacaag atcggttaag tatggtagac gcatttaatg
    43381 ctggcgaaaa agatgttttt ctgatttcct taaaggctgg gggaactgga ttgaatctca
    43441 cgggagcaga tacggtcatc ttgtatgacc tttggtggaa tcctgccatt gaagaacaag
    43501 ctgctggacg tgcacatcgg atcggacaaa aaaatgtggt agaagtgtgg cgtatgatct
    43561 ctgaaggaac gattgaggaa cgcatggatt cactccagca agaaaaacgc gaactgttcc
    43621 aaaaagtgat ccaaggtaat gaagaacagt tgacgaaact gactgaagaa gacatccgca
    43681 tgattttaag tgtaggagaa atcgaggagt aagagactcg aaaaagcgtt cagtaatggg
    43741 taagaaagcc tttctaaaga agcgattgtt tatttgattt aaaaaatagc aaggaggctg
    43801 ggattccatt ttatcccagc ttccttgcta tcattttttt atttctattt ttaaaggtta
    43861 tacaaagatt gtcttcattg tctctaacag ctgatcaaaa gaagaaatct cgatatcgca
    43921 aattggagtc tgttcaattt tgcgttctcg atggttgaac caaagcgctt gccagttagc
    43981 actttttgca cctagaacat cattgtcgta attatcgcca acgtataagg tctcttctgg
    44041 aagcatatga aattctttct cagctagctg aaaaatctct ttttctggtt tttggaaacc
    44101 tgttgcttga gaaatgagca tattgcggga aggaatccaa ttgttcaatt gaagttgctt
    44161 cagttttttt gtctgatggt cagtcggccc atttgtgata attcctaatg ggacattctt
    44221 ttccttcaag aaatcgaggg tttttttgac ctcctcatgc aagcagatat tatcaagttc
    44281 ttcctcgtaa attttttgaa ataagaggcc atcttcttcc gtaatatgag ggtagtctaa
    44341 atcttttagt gattggctga tccgatgtgc tcgcatatat tctagtgtcc aatctcctgc
    44401 cattactttt gggaagtttt catcactatg atggcggaac cggatatata gtggatgcat
    44461 atctgcatct gaaacaagcg gaacgactct tttgaccgcg tttcgaaatg gctgctgctg
    44521 atcatacatc gtatcatcaa catcaaacac aactgattta atcaaaattt tacacgcctt
    44581 tcggttttca ttgatttttt cacaaatcct tgactattgt aacagaaaaa attgtctaaa
    44641 aaagctgata aataaaggtt tttcttacta tatttctatt tccttgacag aataaggttt
    44701 ttatatttca gtttttttca ctaaaaatcg tgaaacacgt caataagttt gatgaacaaa
    44761 ttaatcatta tggtagaagt cgattgattt attttaatgc aatctctgtg gaattttcga
    44821 aag
//
LOCUS       DAZKOR010000014        38723 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000014 DAZKOR010000000
VERSION     DAZKOR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.014"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..767
                     /locus_tag="ACG8ZM_000702"
     CDS             66..767
                     /locus_tag="ACG8ZM_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0491688.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQNPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="ACG8ZM_000703"
     CDS             764..1885
                     /locus_tag="ACG8ZM_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0491689.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="ACG8ZM_000704"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="ACG8ZM_000704"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0491690.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="ACG8ZM_000705"
     CDS             3264..4172
                     /locus_tag="ACG8ZM_000705"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0491691.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="ACG8ZM_000706"
     CDS             4207..4479
                     /locus_tag="ACG8ZM_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0491692.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="ACG8ZM_000707"
     CDS             4490..5536
                     /locus_tag="ACG8ZM_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0491693.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="ACG8ZM_000708"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="ACG8ZM_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0491694.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9233)
                     /locus_tag="ACG8ZM_000709"
     CDS             complement(8547..9233)
                     /locus_tag="ACG8ZM_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491695.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFK
                     SYKLEKVKNT"
     gene            complement(9211..9705)
                     /locus_tag="ACG8ZM_000710"
     CDS             complement(9211..9705)
                     /locus_tag="ACG8ZM_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0491696.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="ACG8ZM_000711"
     CDS             complement(9725..10945)
                     /locus_tag="ACG8ZM_000711"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0491697.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="ACG8ZM_000712"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="ACG8ZM_000712"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0491698.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13123..14034
                     /locus_tag="ACG8ZM_000713"
     CDS             13123..14034
                     /locus_tag="ACG8ZM_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0491699.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            15030..15281
                     /locus_tag="ACG8ZM_000714"
     CDS             15030..15281
                     /locus_tag="ACG8ZM_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491700.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            15394..15636
                     /locus_tag="ACG8ZM_000715"
                     /pseudo
     CDS             15394..15636
                     /locus_tag="ACG8ZM_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15726..15965
                     /locus_tag="ACG8ZM_000716"
     CDS             15726..15965
                     /locus_tag="ACG8ZM_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491701.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            16112..20395
                     /locus_tag="ACG8ZM_000717"
     CDS             16112..20395
                     /locus_tag="ACG8ZM_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0491702.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            20494..20859
                     /locus_tag="ACG8ZM_000718"
     CDS             20494..20859
                     /locus_tag="ACG8ZM_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491703.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            20861..21313
                     /locus_tag="ACG8ZM_000719"
     CDS             20861..21313
                     /locus_tag="ACG8ZM_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491704.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            21762..23237
                     /locus_tag="ACG8ZM_000720"
     CDS             21762..23237
                     /locus_tag="ACG8ZM_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0491705.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            23260..23571
                     /locus_tag="ACG8ZM_000721"
     CDS             23260..23571
                     /locus_tag="ACG8ZM_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491706.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            23932..25188
                     /locus_tag="ACG8ZM_000722"
     CDS             23932..25188
                     /locus_tag="ACG8ZM_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0491707.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            25185..25730
                     /locus_tag="ACG8ZM_000723"
     CDS             25185..25730
                     /locus_tag="ACG8ZM_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491708.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            25731..26231
                     /locus_tag="ACG8ZM_000724"
     CDS             25731..26231
                     /locus_tag="ACG8ZM_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491709.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            26312..26443
                     /locus_tag="ACG8ZM_000725"
     CDS             26312..26443
                     /locus_tag="ACG8ZM_000725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491710.1"
                     /translation="MSLEFLGEPIEKEEFVSHYMDLFIQVIKNFVRLMNFIHANKTI"
     gene            26590..26868
                     /locus_tag="ACG8ZM_000726"
     CDS             26590..26868
                     /locus_tag="ACG8ZM_000726"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491711.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            26888..27232
                     /locus_tag="ACG8ZM_000727"
     CDS             26888..27232
                     /locus_tag="ACG8ZM_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491712.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            27251..28162
                     /locus_tag="ACG8ZM_000728"
     CDS             27251..28162
                     /locus_tag="ACG8ZM_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0491713.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            28241..29233
                     /locus_tag="ACG8ZM_000729"
     CDS             28241..29233
                     /locus_tag="ACG8ZM_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0491714.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            29230..29448
                     /locus_tag="ACG8ZM_000730"
     CDS             29230..29448
                     /locus_tag="ACG8ZM_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0491715.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            29461..29871
                     /locus_tag="ACG8ZM_000731"
     CDS             29461..29871
                     /locus_tag="ACG8ZM_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFQ0491716.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            29923..32451
                     /locus_tag="ACG8ZM_000732"
     CDS             29923..32451
                     /locus_tag="ACG8ZM_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0491717.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            32467..34677
                     /locus_tag="ACG8ZM_000733"
     CDS             32467..34677
                     /locus_tag="ACG8ZM_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0491718.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            34685..35776
                     /locus_tag="ACG8ZM_000734"
     CDS             34685..35776
                     /locus_tag="ACG8ZM_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0491719.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            35797..36339
                     /locus_tag="ACG8ZM_000735"
     CDS             35797..36339
                     /locus_tag="ACG8ZM_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0491720.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            36534..36791
                     /locus_tag="ACG8ZM_000736"
     CDS             36534..36791
                     /locus_tag="ACG8ZM_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491721.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            36805..37137
                     /locus_tag="ACG8ZM_000737"
     CDS             36805..37137
                     /locus_tag="ACG8ZM_000737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491722.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            37182..37730
                     /locus_tag="ACG8ZM_000738"
     CDS             37182..37730
                     /locus_tag="ACG8ZM_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0491723.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            37737..38189
                     /locus_tag="ACG8ZM_000739"
     CDS             37737..38189
                     /locus_tag="ACG8ZM_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491724.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            38286..38627
                     /locus_tag="ACG8ZM_000740"
     CDS             38286..38627
                     /locus_tag="ACG8ZM_000740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491725.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
BASE COUNT        13146 a         6056 c         8217 g        11304 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aacccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatcct ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaatcta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 ttaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc cagtagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggatgagc aagatagtgt gaaataactt ataatcaatc gaaaaaccta ttaaataaac
    12961 ctttttgagt agatgcaaca ctgaggatta tctgtataaa agattttaaa ctgtcatttg
    13021 gttatcacgg cgatactaaa attaacaaaa ttggaatatt tttcttgaag atttttagct
    13081 gtcagtatat gatgaaagca tctcatagga ggtgcttttt tcatggttga taattcaagt
    13141 ggaagggttc ttaaatctct aagaaaagag aaaaaactat ctcaaaaaaa gttagctgat
    13201 ttagcaggta tttcacaaag tactttagtt aaatatgaaa aagggtcaag aaaaatccct
    13261 aaagacgtag ataatactct aagcaagatt ctgaatatag aaactctaat aaaagatgag
    13321 gaagataaga ttgagattct catcggtaaa ctaattgcat acagagatat gaataagtta
    13381 cttaacaaag agttagctga taatatagga attagtgaag tattactcag ttatgtgtta
    13441 aatagaaaga gaaatccgtc aaaagaaatg caaaaaaaaa tcgatatttt tctattaagt
    13501 aatgaaaaag aaattttgaa ggagattaat cgtgatagtg agatatttag tttatcaaaa
    13561 gacgataaaa ttgttatggg aaaaagaata cgagaagtcc gaaagaaccg ggaagagacg
    13621 cttgaaaaat ttggtaaaaa ctttaccatc tatacaggca aaaatgttat aagtagatgg
    13681 gaaaaaggta ttaatattcc agatattgaa aaactaatga atattgccta tcttggtaag
    13741 gtcactgttc cttaccttat gtacggagaa gattataaga atatacttcc aaaagatgaa
    13801 agagttagcg attttaaaaa gataaattct tttagtatgg gattaaggat gaggaaaatt
    13861 aggaaagatt attatctaga aagggaggag tttggaaagc ttttttctcc ttcaatttca
    13921 aaatggtcca tagataggta tgagaatggt agagatatac ccaatactaa tcgaattatc
    13981 caatatgctt atataggaaa tctttcttta gaatttttga tttacgggat ataaaaatag
    14041 tttacgtcga tacttattaa gttatcgacg ttttttatta tagaaaaaat ttataaaagg
    14101 aggtttcttg atatgataag aaacaaaaag ggtttgttga aagttgcttt actggcagct
    14161 tttttctaag taactttttg acaagctcaa tggcaacaca agcggaagaa gtaatacaag
    14221 aactaactgt tatgaataac agctagataa aatgggctat gtatgttatc caacaattta
    14281 gtaatcacta gaagcctagc gaaagttcgc ttgataagtt atagatgagt attgatttgt
    14341 agtgtttcca aatcaaaagg gtacagagcc acatgaaata tgttgtcctc tgcagcgtca
    14401 ggatgggaat ccataccatg gagattcgga gatctgtgag ggaatactta cgtggttaat
    14461 atgtcagttg tgttggacgt agtaatggtc atagtgggta gattgactga attcttatat
    14521 gagaaaatga aaccaacaag aagatagggt tatttataca gaattgggga tcttttctgg
    14581 aaaagtaatc ggataatgcg agattcgtac ggtctttttt aaatttctct aaaaaaaacg
    14641 gcatcgaatg ggttgggtga ttgataagca atgaatcata gactgatata gaataaaaaa
    14701 tgattctatt tatcttttta catctatcgt tttttcaagt agccgcaaat tatatcagca
    14761 ttcaacaatt tttgtataca agttctagta aaaggatctg aagaaaaagt tggttgttgt
    14821 gaagataaaa aaagaatcag gttttactga gtttttcttc aaaatttgtg ttattaggtt
    14881 aggacaaaga taagaagaag tatgaagatt taaaaaagga atgaggaaaa gatagtaatt
    14941 tccgattatt ggagtttttt tatttataaa gaagaagcga ataagttgtg attcctagat
    15001 ttcgcgcgtg attaattaat gagcaaaaaa tgcggaaagt tttagtgttt aatacacgag
    15061 gacaaagttt cttaggtatt gaaggcacac gtgtaacggt tgatgggtgg tgtgattgtt
    15121 tcgaggttgg aaaacatgtt gaacttatgg catttactga gctaaaagat aagaacaacg
    15181 tggaattttt cgagagtgat attggttggg acgatcatga agaagtttat ggaaaattta
    15241 ttttgcggaa gggaaattct tatacgaatg gggaagcata agtagcgatt tgttagagat
    15301 cattgatagg attgaaattg tcgggaatat ttatgagaat ctagagttat ttgaggtgat
    15361 agaatgagtg atggttttga acaagtatca ataattaaaa atcaagttcg ggagattctt
    15421 agaaaaaaag gctatttggt agatagttat tttgaaggag attacgaaac atgggtaggt
    15481 gtctatgctc gtccagagaa taaacctacc tatttagatc ctacgacgtc agaagatgcc
    15541 tatttacaaa ataggtatcg agtagatggt tttaagcaag actttgcgga gtggtttgaa
    15601 tgggaaattg aaaacggaga agtaaaagaa gaataagagt acttgttgta tcaaaataag
    15661 ccagtagaac aaccaaacca gtagctagta atgacaataa tactgaggaa tcggatcatt
    15721 atggtatgat tgatatttct cttccagaag atcttaagcc agatcctgta acaattggca
    15781 atagtgtcat gatttttgat tcattagatg aagctatgtc atatggttgg aaagaaatta
    15841 aggatgaagg tagtaattgg tatggcatga gatttggtga tggtgaagtt gccaaaaaag
    15901 ctggtattcc aattgggaaa tggacagttg atttccacga agcaggttct ttttttgttc
    15961 tgtaattata gtgggagggt aaaatcattg atgaaatgtg ttcagataag agcgccagat
    16021 aaaaatagaa gagacagttc acgtcgttat cacttttggt aacggcgttt tgttttatta
    16081 tataaaattc tcaggaaaag gaggaattct tgtgtatgca aaaacgaaaa tgaattggtt
    16141 gaaagcactc gcattggtgg ctttttctca attctgtttc ttggtgggaa ctgctcaagc
    16201 acatgcagaa gaaacgacgg ctatacaacc tacgatggaa gccattgccc aagtcgcttc
    16261 tgaagtgaca gaagtccaac cagcagaagc cggggtccca gctggtgaag ccactgttga
    16321 acaggcccct gtgacagaag cgccaacggt caattccaaa acgactctcg atcagacgat
    16381 tggggaaaca caacacccag ggaatagtcc tggtacttca caaccagttg aagaaactgc
    16441 ctcaattaca acccctgctt cagaaacgtc cactctagaa ggagaaaaag ctgccgaagc
    16501 aacgccttct gtgcagccag agtcttctga gaaacaagcg gaagaaaaga aacgaataga
    16561 agaaacagtg gaacaaccag cggctactgc tgacaaagaa ggaaaaaatg aggaaaacgt
    16621 tgaaaaaaca atctcttcaa ccgcagctag tcaaaatcaa ccaacgaaac caaacaaaga
    16681 atctacggct agtattgaaa atcaaaagaa aaaagagcag aacattccaa aaacaggtac
    16741 acctacactg ttagtaacag taccaaccaa tcgttcggtt cgtgttcgcc gtgcaaccaa
    16801 tgcagctgtt ttaggtggtc ggaaaattga ttggggcgaa aatattggtg ttacttctag
    16861 tccgaacaat ctaaacttca ctttaaatta tatggttcgt tttgagatta atggacaagt
    16921 ggcgttttgt attgagcctg gtgtctcagc cgcagaaggt caagtttata cagctactgc
    16981 tttgaaaaac tatttgaaaa attcaaatgt acggaaaaaa acaagcttga tctcttattt
    17041 aggatatgtc acaaataaag acaaatccga tgaacaatac attgcggcac aatttatgat
    17101 ttgggaagct ttgggcacaa aaatcccaat caaaaatatc cgtgttgatt atgatcgtcg
    17161 aaaagcagaa attcaacgtt tgattaataa ctttggtgaa aaagcaactt ttaataatca
    17221 aacacatacg gtaaaaattg gcgaaacgtt gaaagtaaag aataaaaatg attttttgaa
    17281 atatgtgaaa gacattcaaa aaccaaaagg tgtcaatgta tctattcacg gcaatgaagt
    17341 atggattaca gtcacaaaag aggcaccgga taaagcaaaa attcaattga ataaaattaa
    17401 agttcaaggt gcgccattag cctatcgtcg tccaggagct caaacaattg gtgtgttaca
    17461 tccaacagaa cctaatgcgt ctgttattaa tttacaaatt ctaaaacaag gaaacattcg
    17521 tgtgtttaaa gaagatgcgg aaactggtgt gaaagcacaa ggtgcagctt ctttagaaaa
    17581 tgcggtctat ggtttgtatc aagcagatgg gaaaaagatt aaagaaatta ccttgaaaaa
    17641 aattaacggc aaagtacagg cagaaattaa agatcttgat cctggcaaat atgttttaaa
    17701 agaaatcaaa gcgccaaaag gttatgtgct ttctgatgaa gcaattgaaa ttgttgttga
    17761 acctggaaaa actgtcaatg cgaccgcaaa agatcaagtg atcaaaggaa ccattgattt
    17821 agtgaaagtt gctaataaag atttagtcga ttcaacgaat ccagataaca aaccaaaatt
    17881 agctggcatt gaaatcactt tgacgtcaaa aacaactggc aaagtggtga aaaccgttgt
    17941 gacagacaat gatggttatg ctacttttgg taaaaatacc gttgtctttg atacgtacat
    18001 tctttctgaa accaaaggaa aagaagggta taaactattt gaaccacttg aagtcacaat
    18061 ttctgaaaat gggcaaactt tccattatgt tttagaagat aaagtaattg aacaacgtct
    18121 taaagtggtg aaagtggatc aagaaacagg aaaagtgatt ccacttgcta atacagaatt
    18181 caagattttt gatactttag ccaatcgtta tgtcacaatg gagattccaa atgatacaga
    18241 aacaacggat atctttaaaa ccaatgaaaa aggctttttc ctaactaacg gtacacttac
    18301 ttatggaaaa gatcgctatc gtttggaaga aatcaaagcc cccgtaaatt acgttttgaa
    18361 tcaaaaccca ttagtgttct cgattaccaa tgatacagaa ccgatcaaag tgatccattt
    18421 tgcgaaccgt caagcacgga aaagtgtaaa attattcaag tatgaagaat ggaacaagaa
    18481 gaaaacgccg ctcgcaggtg tgaactttac cttgtataac ggtaaaaatc aagcaattgg
    18541 tgaatatacc actgatcaaa acggggaaat tttagtgaaa ggtctagttg ccgatgacta
    18601 ttacttcttg gaaaatgccc cattagaaag tttccaacca aatacagata aacaagcatt
    18661 cacggttgca ttcaaacaaa atgcgccaga tgatggggaa acgttagtgg tttctgtacg
    18721 taattacttg attccaccaa aactagaaac acaagcgtca aataaagccg atggtgaaaa
    18781 agtggttgat ccattagaaa cgatcgtcat taaagacagt gtttcttata ccaacttgtt
    18841 tgtcggaaaa gaatacacca tttcaggagt attgatgaac aaggcaacga atgcgccaat
    18901 tttacaagat ggcaaagaaa tcacaagtac cttggccttt attgcagaca aagtcaatgg
    18961 cacaaaagac cttgaatttg tggttaatgc tagtgttctg aaaggtcaaa cgatcgttgt
    19021 ttttgaaaaa cttttccgtg atggcattga agttgcagca catgcggata tcaacgatga
    19081 agcccaaagt gtgaaagtga ataatccaac cgttcatacc acggctgtct ttgaagacgg
    19141 attgacagtc gcagacccaa caggattgtt taccttaaac gataccgttg aaattaccga
    19201 gattcttcca ggaaaagcat acttggtcaa agggatctta atggataaag caaccggtga
    19261 acccttgctt gtgcatggaa atacggtaga aacgagtgtt ggctttattg ccaaagcaga
    19321 aaatgaaacg gtgatcgtac catttacctt tgatttgaca ggtataaatg gtcgtgaaat
    19381 cgtcgtgttt gaaagtcttt ttagaaatga tgaagaacta gcaaaccata aagacatcaa
    19441 cgacaaaggg caaacggtac gagtaactaa cccaagtgta cgtacaacgg ctacacataa
    19501 acccactggc ttgaaagagg ttaatccatt aacgaaagtc accattacag acgctgtgaa
    19561 ataccacgat ttggtcgttg gaaaaacata cacagtcaac ggtgttctaa tggacaaagt
    19621 aacggaaaaa ccagtgctag tagatggaaa agaagtcaca agtaccttaa cgtttgtggc
    19681 agaaacaacg gatggtacag tcaatctaga ctttattcta gacgcacgtg cgttgcgagg
    19741 caaagaagtc gtggtctttg aagatctgtt tagagaaggc gtattattgg cttctcacgc
    19801 agatattacc gacaatgacc aaaccatcaa aatcagtaat ccaaccatta cgacgaaagc
    19861 gacgaataaa aatggcggaa aagagatctt ggccttgcca aatcaaactg tgcttgaatg
    19921 ggtcaaagta gacggtcttg cgattggtca aatctatcgc ttaattgttc aaggctactt
    19981 aacaccagac ggcacaccat ttgaaggcac tcatgcggaa aaagtctttg cggccgataa
    20041 agaaatgatg gaattcttat ttgaattctt agtggacggt cgcaatcttg caggaaaagg
    20101 cttgagtttt gcggaatggc tccaagcgaa aacggaagat gagaaagaaa cgtttgaaga
    20161 agtggcgaaa cacaatgtag atctaacaaa caaagatcaa acggttcaaa ttgtggaagc
    20221 gccaaaacca ccgcaaccag tgaaaccaga acaacgggtt aaaccagcta ctgttgtacc
    20281 aatgttgcca aaaacaggtg aacaaacaag tagtatactt atggttcttg gattagtagt
    20341 attagtcatt gctttaggta taacacttta taaagtacgt aaagccaaag attagtcatg
    20401 aggaacaagc ggttaggatt tttaaccgct tgtttgattg taatgtagaa atatttagaa
    20461 tttattttgg tttttaagag gaggaaaaac tttatggcag aaaaagcatt gcgtatgaag
    20521 aaagagtttt taggtcaagt atttttattg ggttcacggg aacctaatcc accaaaggaa
    20581 attaatggtg tgtctgggcg atatgttcaa ttacaagcag agtgttcgcg agaagaacag
    20641 cctgcttttt ttgtttatct cacagctgaa gaggataaaa gattacgatt accagaaaaa
    20701 gaggtgcgta tttcatttga agaagcttat tttgtttttc gtaaagaacg agtgaatagt
    20761 cgtgaaacaa atgatatggg acaaacagag cgtcgaacaa ctggctatca agatgttggt
    20821 gtctttttaa ctggtgtaaa aattggggag gttttgtaag atgggattaa aatttccaaa
    20881 taattatgac attacagaag cattagatga aacttgttta ggagccgtac gctttggcgc
    20941 attagaagag tctgatcgtg tatttgataa tgaaacaggc gattatacgg gagaaattcg
    21001 atcacagcga gcacaattag tttttggaaa tccagatgaa ccaaagattg cacgacgagt
    21061 taaatttcca gcagatgtag atttgactgg ttttaaacga cgtgatcctg tgaaattaat
    21121 tgatccagaa tatcatgtgg tatatattcc agcacaagat ggcaatcgtg aatattcaga
    21181 tttagtgatt tatgcgaaag gattagagaa agtagaagct ccaacaaata atcctaatat
    21241 gaaacaatca caagaaaaag gtttagatca aaaacaacca agtgttgatt ctaaggaaaa
    21301 agagaaaaaa taaggttttt ttgaaagaat aggaaggagt cttcctattc tttttatcta
    21361 agatagaagg aaggggagat tttgaattca gataaaacca atcaaatact ttgagagatt
    21421 gtgcttgtta tcaaaatatc caacaattga ttgagtattt tggagacaat ccaacgattt
    21481 ttgtacaaac aaatgcatat atttgtgcgg ttattgggca gtgtatgttt ttacgtgatc
    21541 ttgttcgttt ttcattaccg taccagtgga aacaagatca tcaggaagtg atttggattc
    21601 gtatagtacg aatatgtaat atgtgtgcac acccttataa aaatcgatta aatctttttc
    21661 gattatgttt ttttactaaa tgtgatattc ctgtattagt tgctttttgt caaaagtttt
    21721 tagcacagga aaagtaaaaa cagaaaggag gaatgtgaca attgaaagct cattttttca
    21781 agtatcgtgg tcggcgaatt cgctatttca atcgttattt atggttttgc tattatggtt
    21841 tctttttgtt tccatttttg attctgttag gttggtttag ttggaaagta ttttatccac
    21901 gtttaagtac gttttcgaat tggcaaatgt atcttattcc tggaataagt attgtgttgt
    21961 atttgttgct ggttagtggg ttaattttag ggttgatgca ttggtcgcga ctaaaagaag
    22021 gatattttgc ctctgtttat tggcgtcagt tacttgttcg aatgttaatt gacaatcggt
    22081 ttgtttatac aaagaaacaa gttagtgaga aacaaacaag ggaaaaaatg agattaccaa
    22141 aagtttattt ttatcaaaag aaacaagaat tagtggtttc ttttccttta gatggtgggc
    22201 gttttcacga acgattttta aaaatgggtc atttgttatc tgaagtattt ttgcgtgatt
    22261 taattcgtga agaacgagaa aaagcccgaa tcaattatgt attcctttca gattctggtc
    22321 ggattcctat tgatcaatgt atagcagaaa atggacgtat tcatttaatg aaaactttgg
    22381 attgggtata tgatcaatca ccaaatatgt tgattgctgg tgcaatcggt ggtgggaaaa
    22441 cctatttatt gtattcttta attcaagctt gtttaagtgt tgggacagta gatatttgcg
    22501 atggaaaagc ggcagattta gcagcattag gtgacgtcga tgtattcaaa ggacatgtgt
    22561 tttacaaaat aaatgaagaa atgattattt gtttacgaaa tgcattaaaa gaaatgaatc
    22621 aacggtatgc ctatatgaaa accatgaaag atcctaaata tgaaccaggg aaaaattatg
    22681 cttattatgg gttaccaccg cattttatct tttttgatga atgggcagct ttttatggtt
    22741 cgttggatat ggaagcgaga aatagagtcg ataaattgat tcagccatta gtattaaagg
    22801 gtcgtcaagc agggatgtac attattttag caatgcaacg accagatgca gaatattttc
    22861 caagtggtgt tcgggataat ttaacattcc gtatttctgt tggtcgattg tctcccattg
    22921 gttatttaat gacttttgga gaagatttta aaaataagcc attttacaac aaagatcaac
    22981 ctggtcgtgg atatgctttt ccaggatttg atgtacctgt agagtttttt gcgccatttg
    23041 tttacaaaga ttatcacttt atcaaagaat ttgagaaatt tactgaaatg attgtctatg
    23101 attttacagt aaaagaggat agtcaagatc aaacgatggg agatgataat cagttgttac
    23161 aagaacaact agagcgccaa atgtatttag caaaagatcc aaaaggaaca agtttttctg
    23221 atgcgcatat tttgtaatca actaaggagg tgaagttaaa tggcagagag ttgttattat
    23281 agagtagcta cagcaatacg aatgattaat ccttctcttt cttccagaac gttttatgat
    23341 tggttgaatc gtattgaaca agtaactgat taccgctttt tgcgcaaaga acgtgttttt
    23401 acaggaaaag ttattaatca agtattgcta acgaaaaaag atatagaacg attgactcga
    23461 ttgtatcatt atcgagtaga tttagaagag gatttaactt tgtcaattta tcgcgttttt
    23521 tctcctgaaa aatatagtga aataacaaaa ttagatcatt taattttgta gttattatct
    23581 atcgggaggg ctctagacgt agtctggtag ccgcactctg cctggcggtg cgcattaagg
    23641 ctgcgaagca tgggcctata atgcgcactc gctagcagcg tgtgcagctc acaaagaggt
    23701 cgtgcgcttg cgcacggcct ctttgtgtaa agccccccga tatataatat ggggggcaga
    23761 aataacataa cacatagaaa aaagttttat aaaccctttt gtatcaagtg attatctaag
    23821 gtcagcagtt gtactacttt tcccaaaaaa cgagggggac tagttgatat atcggtgttt
    23881 ctaaagggtt aaaaaaaatc gattttgtta ttttataaaa ggagcgagaa aatgacaccg
    23941 attgagctaa aaagctacag aaagaagtta agtgcaattc ataatataag tggagataaa
    24001 aagccgctat cacaagcaag atttgcgcaa ttgatgggct gctcaaaaga ttacattaga
    24061 aagatggaac aagggaaaag aaaaatccca gaggattttg aacaacgaat caaggaacgc
    24121 tttcgattgt atatgaaaag cggaatagat cttcgagcaa tgattgatta tttgcgtctt
    24181 tccttttttg ggacagacta caaaacagtg attcaacgtg tattacgaat ggatctgaca
    24241 gaatttgaaa cacgtccaac gggcttgtat ctttttgact gtctttctgt tcgtgggaat
    24301 atctgggtgt tctggcattc aaaacgaact gtaacagaaa atgtacttgt gcaattttca
    24361 ggacaaggtt gccgggaata tgaggaagtc atgcgtgaac aacaaacaga ttggcgaaca
    24421 tggctatatt ccatttggaa aaatcaagga aagttggaag ctggttatac acgtgtgcaa
    24481 tgtagccgat tcgatattgc gttagatgaa ctttggggat tggaaacgga agaacatttc
    24541 gatctctttg aattactaga aaaacaaaaa gcaggtttat tagaaatgga ctttaagaga
    24601 ttccagaata ttggcggttg ttttttagat gatggctatt ttcgaagtga aggattatct
    24661 ttgtattttg gatcgcgaaa gagtcctctc ttttttaatt tttatgagaa acgttttgaa
    24721 attgcgaacc gagaaaaaat ttcagaatta gaagcattaa cgaaatatgg tatttataat
    24781 cgatatgagt tacggttagc caatgagaaa gcaacacaag cagtagaagc atttatttta
    24841 ggtgattcac cgaaacgttt aggagagatt ggcgttggtt taattaatgc aaaacttatt
    24901 gtttgggata gactggaaag tgatgcacgg attgttgata aacgatgggc aaaattattt
    24961 cgttcgatgg aagaattgga ttttacagta aagccagaaa aattctcatt agatcgttca
    25021 gaacgctggt ttttgaaaca agtagctccg acattaaagt tgttacaaga agcagatcgt
    25081 attttaggaa gagactttgt acaagaaggg attgataatg ccgaactaaa tgacgagcaa
    25141 caaaactatc tagaatggat gggaacgtca ggagggaaaa ttgcatgaat gcatatacga
    25201 caacagaatg gctgcgcctg ttggatttaa aggctgcaat cgaagcatta aatgaaaaga
    25261 tggttgattt gtcttatttt cgttttcgag ttccttatat tgaacaagcg gtaaaagctg
    25321 gacgttatca ggaaaaagag aactggcaag aaattgcacg cttattagaa gttcgtaagg
    25381 gatacgaaca agaactcgaa gagttggaat ttagccgaag gaaaggaagg ctagaattca
    25441 ttcgctttta tcgattttca ttgccaatac ctgcgatttt agcggttaaa aaaggctgcg
    25501 ataaaatgaa gatatatgaa aactgtgtgg cagcacttag cagcgaaaaa cctcttatgg
    25561 aagaaatttc attggtgact gtcacttggg aaatgagtcg ccagccaaca gaagaacaga
    25621 catatctttc tttagaagag attgagatag aattagagga aattggtcgc tatgccactt
    25681 gttctaccta ttgtggatcg gtcattagta ttgctggtgt gattgtctaa atgaaaaaaa
    25741 ttatgattta tgttggtgcg tattggtcac gtgatccaac agtattagaa aatccagagg
    25801 cggtacacta ttgcttgcgc caattattct atctgtataa agaacgatta gaatcgttga
    25861 ttcgtcagtt accatatact gacagacgtt tagatgaact tcttctaagg tatccagcaa
    25921 tgtataaacg acgaaaaaat agattgttac cagaagagta tcccattgaa aaacatgaac
    25981 ttgaagggcg atttgtggct tatttctatg atgatgttcg tatgcgctta gtagaacaac
    26041 gaatggaaat agaaaatgat cgctactatt ttatcaatta ttgtaagaag cgtaagtatc
    26101 aagtaactga tgatttttat gattgtattt tacaagatgg ggaagtgatt ctatccaaaa
    26161 ttgctccaag ttttcgtgaa cttgtgccga ttgatttttt gaaaaaatgt catttgcgga
    26221 ttcttccgta aaaaagagct ataaaaggaa agaaaagtag aagccaaatc ggtttctgct
    26281 tttttatttt ttttaggaag gagaagatgg tatgtcactg gaatttttag gagagccaat
    26341 tgaaaaggaa gagttcgttt cgcattatat ggatttgttt attcaggtga taaaaaactt
    26401 tgtgcgattg atgaatttta tccacgcgaa caagactatc tagccgctga acgaataaaa
    26461 atcagtttat tatgggaaaa attcacagat gaaaataacc gaccgccaga ttatcgctat
    26521 ttatcagatt gtttaacgaa taatgtgctt tctaaagaat acctatttca caaacgtgag
    26581 aaaaatcaaa tgtcggttga acatttttgt gattgttata ttgcattatt gcgtagtcaa
    26641 cagttattaa cagtagaacg atatggtgca gagtttggtt atgtcatgca agctgagcgt
    26701 catagagcta caagatgcta tgaaaaagaa gatcaagtga ttgatggcta tgaagaatta
    26761 tgtattcgaa acaatcgcta tttgcaacaa cgtttatatg aacaattgat gatggatttt
    26821 tcaaatctct atagagggta tgaatgttcg atgattcaag aaaggtagaa gaaaaggggg
    26881 aatttttatg tggcgattat tatttttggt ggtatgtggt atggcattgt tctatgtgta
    26941 tcgtgtctat ctctatccac gaatggaacg atggacaagt tggtatagag atcctttaaa
    27001 acaacgaaaa ggattgaagc ggtatcgtta tgaattactt gttgaatttt cggatgggcg
    27061 acaagcacag tattattttt ttgcaaacta tccaaaatac aacaccagtg aactattgat
    27121 tcaattattc aaaaataatc catatgttgg gttagaagaa aacggacggg agttctttta
    27181 tcgttcagat aatattagtg cggcgtctat gaacaaaaca cgaattgcat aaaaaattgg
    27241 aggaataatc atgaaaaaaa caacattagc agtcgtaaca acactaggaa tttcattatt
    27301 ttcgtttcaa ttagggagtt cattggttta tgcagatgaa actgtagaaa cacctaagtc
    27361 agaaattgta gaaacattgc caggggaagg attaccagat gttgagacag agctaaatgg
    27421 aggaacaacg gaaaaaccag cagaacctga acaattgcag tcagagaaac ctgaaaaaga
    27481 gccagtgaca ccagaaccag agtttcctga atcaacttta ccgactgatt cgatcgaaga
    27541 agaaggtgtg catagttcaa caacaacgga ttcaagtata gtcacaactc cggaacaatc
    27601 aggaacaacc agttcttctg aaaagaatac tgattcgtca agtacggaac agccaacgat
    27661 gtcgtctacc agtgaagaac aaaaagaaga accgaaacaa cctgttgaaa aatcagaagc
    27721 aattccaaaa gaaaataaac ctattgttcc agcaaaagaa gtcacagtat cggtaacccc
    27781 aagcggtgaa attacgacga atacttctca aggaatgagt gtaccgattg tcactagtaa
    27841 tgtagaggaa ttgacccata ttccaacacc aaccacccca ttaaaagtag caggtgatca
    27901 aacaattgtt ggtgtaaaag atggtattcc attaatccaa gatagtgaag ggaatttggt
    27961 aaaggatttg tcgattcctg taaagaaact tccaagtggc aatattgaaa tcaaaacagc
    28021 agatggaaaa acaaaagtct taccaaagac tggtgaagaa atacatgttg cactttctgt
    28081 tctaggagga gtgttgacaa gtattgctgg atttgttgga tataaaagaa agagaaaagg
    28141 aatagaaaat gagggtaatt gaatggacta tttttcagaa aaacaagctt atcgttgtca
    28201 atcgtacgta ctagtaaaaa catactagaa ggagtagctt atgaaaaaac gacaactacg
    28261 gcggaaagaa aaagtttata cagagaaagt accgaagcaa aagaaaatcc attcaaaaaa
    28321 agcagtgaaa tggatttggt gtttggtttt atttctttgt ttttcagggg gcttggcatt
    28381 ttttcaatcc atgaaaacga aggttcaagt gtcgtttttg aaagatgaaa tccaacaatt
    28441 acaaaagaaa gaagtacaaa gtcggtcaga attaacgtac acacctgaaa ttgaatcctt
    28501 tatgaatcgt tttgttgctt tgtatatgaa tacattcgat aatcatgaac aagaacaaca
    28561 acgtcaagaa acattaaaaa aagagtttta tgcacaaaac tttattcaag aaccaggaag
    28621 tgtttcaggt gttcgaaaat taactgctgc ttcgtttatt ggattaaaag aaattgaaac
    28681 cattccaact gctagttata aagtaactta tgatattcgc tcgatgaatc cagagagtaa
    28741 gaaagaaaca agtgtgacaa tcacacaagt actcaatctt cccttttcta tttcaaacaa
    28801 gggctgtcgt attattgctt atccttattt tacggcacta ccaaagaata cagaaaaaca
    28861 acgaatgatt acttatgatg acacattata cgaaacggtt gatgcgtcgg aagcgaaaga
    28921 tgttgaagga tttgtggaag atttcttaga taagtatgcg aaaaacaatg caaaagatat
    28981 gtcatacatg atgaaagaac ctgaaggact caatcaaaca gctaaagttg atcaagtaga
    29041 gatgaaagta ttgcgttata aagaaacgaa aaaacctttt attgtaaaag caacggtcgt
    29101 ctttcgagat ggtgatacgt cacttttaca aaaagaacaa atgacgattg tcattacgaa
    29161 aaaaacaaat cagtattatg ttgaaaaatt aacacataat tggttggata ggaaaggaga
    29221 agaacggaaa tgacactgaa gggaattgta gattttatat taacgcaagg tggctatttt
    29281 ttaataatgg tggttgttta tctgggattg cgaaatttta tgaaatcaaa agtaggaaat
    29341 gcattaatat ctatcgcagc aggtgccgca gtttatttct tcttaaatga tccaacacgt
    29401 gtatttgatg cgtttggtaa aatcattgaa aaattattta tggattagga ggatagtgaa
    29461 gtggattggg aaccttatga ttattcgatt gtttatgcgg aaccttatcg cgtacaacag
    29521 attacaagca attggcgtct tccatttcca gttgcattag tcaatgcagc aacttttttg
    29581 ttggtcgtaa tctttgaata tttattttta cgttcattta ttggttgggt catttctttt
    29641 tttccaccag caggagttgt tttttatata ggactcccct atttatgtgt gttagcactg
    29701 gcaaaactaa aacctgatgg aaaaaaagtg catttttatt tatgggattg gttttgttat
    29761 atgattcgta tgatgaatgg tgattgtgtg tattataact gggacaaggt atcaaacaat
    29821 gcattggaag aattactttt tgtgaataag tatgtttatt tagaggatta gaaaaaggga
    29881 caatcaactg gttgttcctt ttttattggg aggtgaaatt cattggtgct tgtaaaagaa
    29941 ccagtaaaag cattcaagaa aaattgtttg ttaaatggag aggattgtat ctgggcctac
    30001 tatcatgttt ttccacgaac aatttcaaaa aacacaccaa aaaagacaca ggaaataaaa
    30061 gaaatgatgg ctcgtttact gttgctttta gcagattatg aagatgtgga gttggtgatg
    30121 tatccacgaa caatgaattt acggacgaag tttgacacat taggaaaggg attttctccc
    30181 gatagtcaag caattggtgc gtattatgca aaagaaacag tgacattgtt ggaacgagaa
    30241 ggtcaagtct atcgtccttc atttatttta ggaatcaaag tcaaagaaaa agcaacggtg
    30301 gagagttttc atgatgtaat aaatctaatc tggttagatt tagcaggaag tttagcacgc
    30361 ttatttggat tagaagtaga tgatttggaa actttgtttt ctgaaagtga tccttatgta
    30421 aaagaagtga cacaattact ttatagcttt tctattcgtc ccttagtaaa aaaagagctg
    30481 ttgtatctca atcgtctgca atatattcga ggaatggctc acaaagtaga agaacaaaat
    30541 aattgggaag tactgaaaga caatcaagga acgtatgtac gtataccgaa ggaacataaa
    30601 ggccttcttc aattagaaga tgagttaggg agtagttatt tagcattgtt gcctattggg
    30661 gaaacacctg aaaatatgag aaattgtgat ctatttcaat ttgctcagga tctttctttc
    30721 ccagcagaat tgcgaatgaa aattcactat ccacgcttga aaggacaggg aggtattggt
    30781 ttaaaactga attggctatc ggaacggttc aaaacagagc ataaggaatt ggttcaaaat
    30841 gatgaagaac ctagtgaacg cttattaact gttcgaggat tagtaaaaca tttaaagcaa
    30901 cgagtagata atgggcaacc tgtcgtcgat tggatcgcat gttttattgt atacggtcaa
    30961 acggttgaag aagttcgtca aaatagtgca acggttattc aacaacttca tacgattatc
    31021 catatccacc gtgcaaaaaa tgatcaacga gaattgttct ataaatgttt attagggcaa
    31081 ccattgggac ggcagttaca ttggaagcac cgaacaactg tagaagcagt tggcgaatgg
    31141 ttgttttcag cagtatcaga attaggtatg gaatccggct ggtatattgg tcggcaagat
    31201 acattagggg atggtggttc tcgaggagaa aaaacaccat tagaagaatt aatttatgcg
    31261 tcaaatcgtt ttgtcttttt gaatcttcta gccattgcgg aaggaatcaa aggtgcgttg
    31321 tttgatgcgc ctcatgtagc agtaactgga aaaaccggta aagggaagac ctttttagtt
    31381 ggcttgctct ttttgtatag ttcgtttatg aatgtacaaa gtttatttgt ggatcccaaa
    31441 ggagaaaagc gcaaatggtt tacgaaactc attcaagacc cgtattatca gaaacattac
    31501 cctttgtttg tagaacattt gcaaaaaatg agttatgtca cgttagatgc tacaaatccc
    31561 cgtaattatg gtgtattaga tccaattgtt tgtttatccc gagtagatgc gaaacaagta
    31621 gcccaggata tgattgatga gttaagtccg ttaggtacca atcacctttt aaaaggggcg
    31681 gtattaaagg gaatccaaga agttttagtt caaaaagaag ccggacaaca agttggcttg
    31741 atgcatgtgg tcgactattt agaacaaatg gaacaaaaag aggttcgaga atatggtgag
    31801 tttttacgct tgacggtaga agattcgatt ttacgtttag gattttctta tggagaaaac
    31861 ccgggattag actttaatgc caaaacaacg attttagaga tccaagattt gaaattacca
    31921 aatgacaatg tccgaccaga actatataca gatgcggatc gaaagtcttt gtgtttgatg
    31981 attagtttag gtcgtttttg tgaaatgttt ggaaaacgag atagtagtaa gaaaacagcg
    32041 atttatttta cagaagcatg ggtatttaat aattcgaacg caggacgtag tattattgca
    32101 gcaatggcac gtgtggggcg ttcccagatg aatcaattag tgttagatac acaatttatt
    32161 ggtgacttag gttcagataa agaaaaaggg aattttggtg tcgtctttgc ttttgatgaa
    32221 gacagtgagc gagaaaagat tttgaatcat ttacgtttgg aagtaaatga agcgaactta
    32281 gctgaaatga gccacatgat taaaggacag tgttggatgt tagatcccta tggacgtgta
    32341 ggaaaactca atgtacacag tttgtttgaa gaaatgaccg ctgcattaca aacaacggaa
    32401 aaaacaagtc actctcaagt agaagaacag gatacgttaa tcatgcaata gaaatgaggt
    32461 gaaacaatgg cttatataaa gcataaggaa aagaaatggt gcgttttgtt ttttttaacg
    32521 attggattcg gactgctttt tgttggtgga ttggtgtatg cggcagaaaa gcaggtggat
    32581 caatcgatct atgagattgg gaaatttcat attgatacct tttcttctta tttatctgat
    32641 agtgaatttt cattaggcaa aatgttattt ggggcaaatg gcacagattt tctaaaaatg
    32701 atgacgaata tgttctttag tttaagtaaa ttgatttggc aaggccttga ttttgtcatt
    32761 gagaagctct atgaaggagc tgcaatgaac gagttaattc accagttttt cactttttcg
    32821 gatactattt attcaaaaat atacgatcct attggtgcgg ggattgtcgt cttgtatgtg
    32881 ttatatctat ttactcttcg tatatttaga gggaaacatt acgcaaaaat ggtgtttatt
    32941 cgatttctct tcgttgtcat tttttcaatg gtttggtttg gctatggtag taacgtacct
    33001 agtaaaggag aagcttttac caaagggtta aatgatttgt ccgtggaggt agaaggcttt
    33061 attttttctg ctacaaatag tctagatgaa ttggcgacaa aagatgtcaa tggtgtttta
    33121 gtgccaacga cacaaaagga agccattcaa aaagtgcgcc aactttatta taaagcggct
    33181 gttgttgatc cgtatatatt gttaaattat gggacaacaa atttagaaga attgtatcaa
    33241 gcagagattg aaccacaaga atttctagcc aaggaagcgt caagtgaaga attatctgat
    33301 attgagacaa aagtgaccac tgctgcagga gaagatagag ggaaaaagaa ggcttatcgt
    33361 gtctatattc aaccaactaa agctgtttat aaagcagtcg ttggttctat ggtgccattg
    33421 cttaatctaa gtattggatt accacttgta gtgattggca tgattcgttt tatctttcaa
    33481 atgttggtgt tgtgtttatt acttgtgatt cctttttcat tactcgcttc ttttgttcca
    33541 gtatttgatt atatgctcat gaatacctta aagtcctttt tgggcttgat gtttcaaaaa
    33601 tctttttaca gtgtgattat tttggtggca tttttgatct ataacatagt agacgattta
    33661 attccaatga atactgtttt tggtttttta ggaaatatgt ttgtcaaagc actcctatca
    33721 attttactca ttgtaaaacg gaaagattta ttaaagaaac tagggttagg agctgcagat
    33781 caaactataa acaacacgaa aaacatagca agtcattctt tgtctcaaat gaaacaactg
    33841 cctggtcaac tatcaactgc tggacaaaat ttggcaatca aaggtgcagg attagcaggg
    33901 aatgtgtatc gagcaacgaa tccactcgct tctgcagcga tacatggatt tcaaaagttt
    33961 caacaaaaac gtacaccaca agaatcggta aaagggaggc aagtggttaa tccctttgaa
    34021 ctaggtgggc gttcaaatca agccttgcct tatagtggtc gtcatgttcg ttatcatggg
    34081 gtagagcctg aaatgtcttc taccaaatcg aaaacagagt ggaattctgg tctttcaaat
    34141 ggtttgattc caaattcgtc catgaattca gtaagaccaa aacaacaggt acaatcattg
    34201 gcacaattga gtaagcaaca agggaataat caaagaaaac cattgttaaa taaaggtatc
    34261 gcttttgttg gtcaatcgat tccgttgcct tctggaaaag tacccgtgcc acaattaaca
    34321 gctgctggtt ctctttcaca acagattcgc caagtgaaaa atcaggcgaa aaatgggagt
    34381 tatccagcaa tgcagcagtt attgaaccaa tactctgttc ctaattcgtc ggtagccact
    34441 ctatcaggaa atcgttctgt gcaacaaccg gggcaacgtt cgtctgaaag taaaccgtcg
    34501 atttccgcac caaaatcgtt acaacctgca ttttcttctt tagataagcc aacagagcaa
    34561 tcggaaaaaa tagaaaagga tcctaaaaca ctgccgattt tggacgcaaa accaggaact
    34621 gattttactt taccaaaagg acgaacagtc caaaaaccat tatcgaagaa aggatgagga
    34681 agaaatgaag aaagggtgtc taggttgtct tcttttcttt attcttccct tttttctatt
    34741 gccattcttt ttttcaggag aactttctga gcatggagga gaagctacag gtctagctgt
    34801 cgcttttaat ggggaatata ccgaaggatt accgattttt tcagagatta aaggacgtgg
    34861 tccaatcaca gatgaacatg cgcaagcagc agttggtgca gctgtaaagt atcagttatt
    34921 accgagtgtg attctttctc aattaggcta tgaatcagct tatggacagt cacaatctgg
    34981 aagaaacgat acgaactttt ttgggattac ctggtttcct ggttgtccct acccacaagg
    35041 ttctgcacgt ggaattggtg gttctgaagg tggtttctat atgaagtttc cgaacttaaa
    35101 agcttgttat gactattatg gctttatggt ggcgagccaa agcaacttta atcaatgtgt
    35161 gggcaataaa gatcctggaa gttgtttatt gattctaggt cgtggaggct atgcggcagc
    35221 aggtattacc gaagggagtc cgtattatcg aggagcaatg agtatcataa cgtcctataa
    35281 cttaacggaa tatgatactt ttgcgatcaa gcattggaat agcctaccaa caaaaaaaac
    35341 ttcgcttatt gcaggaaaaa gtaaaggcaa tgcgaaggtt ttggatcgga tcattggacg
    35401 atccgtaaat aatggggaat gttatggtgg tacggcatgg tatgtatctc aattaggtgg
    35461 cccacagtta atgggttctg gacacatgtt tgcgatgtta ataggggaag attataactg
    35521 gacaacctat ggttggcaag taatcatgaa cccaaaacca agtgatttac aagcaggaga
    35581 tgtggtgaat tggcaagcag gtggtgcatt gtctcctgga atctatggtc acaccggaat
    35641 tataacagat gtttcatatg gcggacaaca tttctctagt gtggaacaga atgccgagca
    35701 aggacggatt atggctcggt atcaacgcac gtacaatcaa acaaaaattc gtagtctggt
    35761 tcgtaaggtg aaataagaaa ggcagtgact agaaacgtga aaaaagggat aattttagta
    35821 tttagttggt tatgtttgat aggtcttact ggttgtcatt ggttttcttc tgaaaaacaa
    35881 aaaacacaat tgagaacgac agaagaaaca atcgaagcag taaaagaaac aagtggaact
    35941 cttcaaagta ctgaaagtaa cagtcaaatt ggttcattag aagacgagac agcgcaacaa
    36001 atgcaaatag atgaaatgac aaaaaatttc ttaaaagatt ttgctagaaa atggagtaat
    36061 ttcacagatg tttataaacg caatcaagct gtgagggact atatgacaga tcgttgtatt
    36121 aaagacaata gtattgatgt agacccgcat gtacaaatga aaacaactgg gaaactttac
    36181 caggtgacac aagatataga gaatccggat caatatttgt tatttggaga agaaacaatg
    36241 aataaggtta ctcaatttgt attacttcaa ttgaaagtag atcaagaagc tcaaaagatt
    36301 gatgcgatca agatttatta tgtacggagt gcgtattaaa ataaaaaatg aacactcata
    36361 tttattatac accgaagaaa tagaaaaaaa cagactgttt ttttaagaaa aactatgtca
    36421 ttctttactt gaaaaaagtt gttataggac agaaaggata aaacaaaaac ggaaaaaata
    36481 tgtcttatga ataactataa gaactattct attttttagg agatggatac gagatgaaaa
    36541 aactaattgg ctatagtgtg atgtgtattg gatttgttgg tgtgggattg tttgtatcga
    36601 ctgaatctgc ttctgcggct gaatggtata atcctatctg gtataatccg aacattgaat
    36661 gtcaaaaagg ctatccgtat ggaccgacaa atctgaaaaa tggaaaatgt agagtaaatt
    36721 ggaatggcgt tgtagatagt gtaattactg gttcaatgga ctctgctcta catggcttta
    36781 cgggtcacta gaaggaggaa gaaaatgtct gaagagaaaa aagaaatcgt tgaatcatta
    36841 gtagctttaa aagattctct ttctaagatt gaatatgttg ataggcaaga aattcaaaaa
    36901 ctaattgatg atactattat tgaaatccaa gatgcgcgtt gtgaaggtat aaaaatttct
    36961 gtagctttat ctaaagtaat agaaaaaatg aaccgttcat tagcgtttaa tggattgaag
    37021 ctagatagac aaactagtct gatatgggat catttaaaag acttgtatga caagtctaaa
    37081 agaagtgaac ggactgctgt atcaatctta aaaggccttt ggggaatgaa ttcataaaca
    37141 agatatttaa aaagtaactt attttttatg gaggagaaat tatgaaaaga ataattaaaa
    37201 tatttggtgt tttaggtgtt ttcttattag gtttaagtac ttttgttagt actggaacaa
    37261 gtgtgtatgc cacagaaaaa gtgaatacag ttgatttaga aataaagaat aaattagtag
    37321 atgaattaac tttttatttt gatacagttg gttttttaga cgaacatggt gtataccata
    37381 taaaggattt tggtgctatt cgtgaacgaa tcagtcaaga taacgaacaa ggaagagcag
    37441 caagagtctt ttatgaacat attattgttc ctagaatgga gaaagttgat tggaaacgat
    37501 atgctggatg tgtagccatt aattctcttc ctggaggagg agtggcttgg gctataggta
    37561 cgcagggaga ttttattgaa gcattaaaag cttttaattt tgaaaaagct gcggatttat
    37621 tattaaaaat tgcagaaaaa caattaggaa aagctgcagc taaagagtta gctaaggtaa
    37681 atattgtagc aggaattgca gcagctgctg ctagctgtgc attagagtaa aagtttatga
    37741 aaagtttaga acaagagcat atggctttta aacaagcaat gtttaaagaa aatatctatt
    37801 taaaccataa ctatattcgt gtttctaagg catgttcacc agtattgaat atgcttggag
    37861 gtggaaatgg attatatcat ttactgtttg tcgatgtttg ttggttaatt tttttaccag
    37921 atgaactagt tatagtcaat gaaaaaataa caagtaaaaa tgaagtgttt aattattctt
    37981 taacaagaat taattacaaa gagattacca agttttcact agaaaaagtt cctttttggg
    38041 gagaattttg tttaaaaatt aaatgtaact ggaaaagaat gtatttctat attgatggtg
    38101 atgatgctct tacattcggt aaaactactt tttctagttt taattttcag tttctattaa
    38161 aaaataattt ttatggttta ttgaaataag gttgcgaagg aagatgtaga gttatttgca
    38221 ctgttgtgtt ggtatatctc ttaatgctgt gtctttttgg ggagattaat tagtggagtg
    38281 attatatgag tatagatgat tgtttagaag aacaaataag attattttta aagtttttaa
    38341 aagttaaagt tcaaaataac tacttcacat gttatagtta tgcacaccct gttttaaata
    38401 gtcttccttt taattcttta gtgcaaggat tgattacaaa tattgtttta ttagtctgta
    38461 caccaaatga aatatttgta aaaaagatag gcagtggtat ttcttcagta agattaaaag
    38521 agaaagaata tactcaaaag attattcata ttcctaagcc tgaaattaag cgttttgaga
    38581 taaaagacta ttctgcttta gggctgttct caattggtta ctggtgataa aaaataagaa
    38641 tactaaaact atacgtagaa tataagtaat cggcatcaat cctaattatg tgattgaatg
    38701 ccgatttttg ttacgcgcta ttt
//
LOCUS       DAZKOR010000015        38155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000015 DAZKOR010000000
VERSION     DAZKOR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.015"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1071..1397
                     /locus_tag="ACG8ZM_000741"
     CDS             1071..1397
                     /locus_tag="ACG8ZM_000741"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0491726.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            1405..2313
                     /locus_tag="ACG8ZM_000742"
     CDS             1405..2313
                     /locus_tag="ACG8ZM_000742"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0491727.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            2939..3193
                     /locus_tag="ACG8ZM_000743"
     CDS             2939..3193
                     /locus_tag="ACG8ZM_000743"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0491728.1"
                     /translation="MIKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESL
                     DSFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            3735..3893
                     /locus_tag="ACG8ZM_000744"
     CDS             3735..3893
                     /locus_tag="ACG8ZM_000744"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0491729.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            3965..5190
                     /locus_tag="ACG8ZM_000745"
                     /pseudo
     CDS             3965..5190
                     /locus_tag="ACG8ZM_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5279..5671)
                     /gene="rpsI"
                     /locus_tag="ACG8ZM_000746"
     CDS             complement(5279..5671)
                     /gene="rpsI"
                     /locus_tag="ACG8ZM_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0491730.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(5687..6115)
                     /gene="rplM"
                     /locus_tag="ACG8ZM_000747"
     CDS             complement(5687..6115)
                     /gene="rplM"
                     /locus_tag="ACG8ZM_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0491731.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(6649..7116)
                     /locus_tag="ACG8ZM_000748"
     CDS             complement(6649..7116)
                     /locus_tag="ACG8ZM_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0491732.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(7236..8774)
                     /locus_tag="ACG8ZM_000749"
     CDS             complement(7236..8774)
                     /locus_tag="ACG8ZM_000749"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0491733.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            8854..9540
                     /locus_tag="ACG8ZM_000750"
     CDS             8854..9540
                     /locus_tag="ACG8ZM_000750"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0491734.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(9741..10268)
                     /gene="msrA"
                     /locus_tag="ACG8ZM_000751"
     CDS             complement(9741..10268)
                     /gene="msrA"
                     /locus_tag="ACG8ZM_000751"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0491735.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(10352..11029)
                     /gene="pgmB"
                     /locus_tag="ACG8ZM_000752"
     CDS             complement(10352..11029)
                     /gene="pgmB"
                     /locus_tag="ACG8ZM_000752"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0491736.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11026..12154)
                     /locus_tag="ACG8ZM_000753"
                     /pseudo
     CDS             complement(11026..12154)
                     /locus_tag="ACG8ZM_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(12154..14337)
                     /locus_tag="ACG8ZM_000754"
     CDS             complement(12154..14337)
                     /locus_tag="ACG8ZM_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0491737.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(14334..15359)
                     /locus_tag="ACG8ZM_000755"
     CDS             complement(14334..15359)
                     /locus_tag="ACG8ZM_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0491738.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(15404..16231)
                     /locus_tag="ACG8ZM_000756"
     CDS             complement(15404..16231)
                     /locus_tag="ACG8ZM_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0491739.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(16228..17295)
                     /locus_tag="ACG8ZM_000757"
     CDS             complement(16228..17295)
                     /locus_tag="ACG8ZM_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0491740.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(17321..18157)
                     /locus_tag="ACG8ZM_000758"
     CDS             complement(17321..18157)
                     /locus_tag="ACG8ZM_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0491741.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(18154..19023)
                     /locus_tag="ACG8ZM_000759"
     CDS             complement(18154..19023)
                     /locus_tag="ACG8ZM_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0491742.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19093..20376)
                     /locus_tag="ACG8ZM_000760"
     CDS             complement(19093..20376)
                     /locus_tag="ACG8ZM_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0491743.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(20390..22075)
                     /locus_tag="ACG8ZM_000761"
     CDS             complement(20390..22075)
                     /locus_tag="ACG8ZM_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0491744.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            22350..23375
                     /locus_tag="ACG8ZM_000762"
     CDS             22350..23375
                     /locus_tag="ACG8ZM_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0491745.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(23970..27623)
                     /gene="rpoC"
                     /locus_tag="ACG8ZM_000763"
     CDS             complement(23970..27623)
                     /gene="rpoC"
                     /locus_tag="ACG8ZM_000763"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0491746.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(27702..31319)
                     /gene="rpoB"
                     /locus_tag="ACG8ZM_000764"
     CDS             complement(27702..31319)
                     /gene="rpoB"
                     /locus_tag="ACG8ZM_000764"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0491747.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32047..33015
                     /locus_tag="ACG8ZM_000765"
     CDS             32047..33015
                     /locus_tag="ACG8ZM_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0491748.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33033..33938
                     /locus_tag="ACG8ZM_000766"
     CDS             33033..33938
                     /locus_tag="ACG8ZM_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0491749.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            33935..34741
                     /locus_tag="ACG8ZM_000767"
     CDS             33935..34741
                     /locus_tag="ACG8ZM_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491750.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            34898..35866
                     /locus_tag="ACG8ZM_000768"
     CDS             34898..35866
                     /locus_tag="ACG8ZM_000768"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0491751.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(36183..36707)
                     /locus_tag="ACG8ZM_000769"
     CDS             complement(36183..36707)
                     /locus_tag="ACG8ZM_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0491752.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            36994..>38155
                     /gene="scm"
                     /locus_tag="ACG8ZM_000770"
     CDS             36994..>38155
                     /gene="scm"
                     /locus_tag="ACG8ZM_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0491753.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTS"
BASE COUNT        11002 a         8056 c         7102 g        11995 t
ORIGIN      
        1 cctctttatg gtcttgtcct tttttgcctt caaaatattt aaataaaccc ctttcaaaag
       61 catttctagg ttttggacaa taaggaatga cttttttctt catataaata cctcatttaa
      121 ctatttgata tcatcacatt atcaaaaaaa ataggttttt tattaaaact atcttgacct
      181 ttcgggagat ttcactgtga tttattttta ttcccatact tttttcgttg gtcactttcg
      241 tatacctaat ctaagcgatc atcggcgacg gtgcttttac aacgaatggc aggacaaaac
      301 aaggggcgga aaaacttttt tgcctatttc ataaaaaact ttttcctaca gcaatccctt
      361 gttttgtcct accacacgtt gtgaacccaa agcacgccga cgaacgctta gctatacgaa
      421 aatgacctcc aaaaacaaaa aagtgaggtg aatagaaaca aaaagaaatt gaaatctccc
      481 aaaatgtcgt ccgcctcgga gacaacttgc tgctgaaaat ggttggttaa ttcatttaag
      541 tattaaataa gataaatcat caaattaatc ggaaaaatag catgattggc cttatcaaaa
      601 ataaactgta aaaacttata tttctatgag tggtggaaca atgctaatac caaattaatt
      661 tatcgattac ccacctgcac aaaccaaaga tagcattatt aggaaacgaa tcaatcagcg
      721 aatccagcat tataatgagg atactaaaaa taaagttttc ataattataa atccgccttc
      781 ctacgaaagg caccaatgag gtgatgaaaa ccttagagcg ctttctttct ttttttattc
      841 cgtgttaccc tttccttgca ggtccctggt acactacaga acccgaggag gtgagtggcg
      901 gggctgtata gcggcaaccc cgcataatta tatggtgtcg gtcatccgtt aaggcatcaa
      961 tgaatggcgc aaacccgtag cccgtaaact tatctcttcc gaagttgact cgatttcgcc
     1021 ggatgaccag tccctaccaa acctgtacta tttttttaca gaaggttgct atgttcaaaa
     1081 tctttcgtaa aaattgttgt ggcttggatg tccacaaaac gtggatctat gcgtgtatcg
     1141 gaatcacaga tgcgaatggt cgtacagtgt ataagcaagc tcgcttctct tcctttacaa
     1201 aaggtctcaa tgaattagct gaatggcttg ctaaatacaa ctgtaaggat gtgtgtttgg
     1261 aatcttccgg taaattttgg attcctgttt ttaatattct tgaaaaaact tgttttgtca
     1321 cactggctca tccgaagtac accaaacccc agaaaggaaa taaaacagat cgcaaagatg
     1381 caaaaatgga tttgtgatct ctttatgtgc gacatgatca aacccagttt tattcctggt
     1441 cttgaaatcc gccagcttcg tgatctaatg cgttatcgga cgaaactgac caacatgaaa
     1501 acaagtgaaa agaatcgtgc tttaaactgc ttaaccgttt ctaatctgaa actagatgat
     1561 gtttttagtg atgtgttcgg aaaatcatcc acttctatca tccaatactt gttagaacat
     1621 cccggtgaat catttgatgt tcgaccattt atccatgccc gttgtaaaac tcccgttgaa
     1681 gaaatccaag cggcagttga gggggcgatt tctgtacctc aagcgattaa gcttcgccaa
     1741 tgcctcaacc atattaacga gattgagaag catcgagaag agatcaatct agagattcaa
     1801 gctcttgcag aacccttctc ccctgttttg gaactacttc gtaccatacc gggcttagac
     1861 actcagccaa taacagcgat tgcgattctt tcagaaatag gaccggatat gtccgtgttt
     1921 ccaacttcga aacactttat ttcttgggct ggatgctgtc caagaaacga tcgcagtgcc
     1981 cgacgaacaa aatcaacacg tatctctcgt gcagggcagt cgttgaaacc gctacttgta
     2041 cagattgcca atgctcttct cagatccaaa gaacatccag aatttaaaaa tcgctatcgt
     2101 agaatcaaag cgaatcgtgg acacaagaaa gctatcattg ctatttgtaa gatgctcctg
     2161 accgctatct ggaatgtctt atcaaaggtg gaaccctatt ctgctaaagg atatctggag
     2221 ccaaaacctg cgaaagaaga gaaagttctg acgcaatccc aagctctgga ccttttaaga
     2281 aaacgcggat ataccattat agatagtgct taaaccaaag ttacgttgat tcttcctaaa
     2341 tagactcgtt gtcttccatt tcaaaaattt tgaaaacggt aggcttattt agtgtaccgt
     2401 tcttcaggta tcttcaaata gatttatttt caaacttatt tcctttcttc ttttactctc
     2461 ctatagctta gcatttaagt gggcactcgt gtcaccttgc tagatagcta tgaatgccgt
     2521 aataagcctc ctagtatata ggcagactgc caattataac tgattcaatc cacattatga
     2581 taaaatccac cttttcgttt catatgtgtt gaaatatcac atatgtcaaa agtgcacgag
     2641 tcttttcatt cagtgctttg taaatactca aaattattcc ttttcacttt aaaaaaaaga
     2701 taattagtac aaaatcagaa atgaggaaag tattaggatg gactagaaag gagttgttga
     2761 atttgttgca acagattggt catttatcaa ttaaaaatta ggtgaagttt tattcatcac
     2821 tgcagctgct gtttcccaga gaataaaacg tctagaagaa actggtcagt tactgtatta
     2881 tcaagcagca attgactacg aacaagcttg attactaacc aaagcgttta tccacctgtt
     2941 gattaaatca aatcaaaaat caggttttta tacgttcata aaagcaatct ccagtgtact
     3001 cgaatgtgac tgtatcatag gtccttattc gatgttaatc aaaatggggt ttccttctac
     3061 cgaatcattg gacagtttta tcaatgattt atcaaagttt ggacgaacca acacccaaat
     3121 cgttttctcg actcttgttt ccttaatggt ttatcagttc tggaatgttt atcatgcgct
     3181 tttattaaaa tagaaaagag cttttttcat gcgacaaagc agtgatcatt tattggcaca
     3241 tgcaatcttt gtggatccat tttttgaaaa tacgatattt agttttgacc caaaaatctt
     3301 taaatacgta tttgataatt tatcttaaca accagataat tttattcagc accgtattat
     3361 ctatattgaa ttctcggaat caagataaat ttttttccgt ttttgtttct tttttaatct
     3421 tcttcttgtt attaatagtg attaggatga tattcctata ttgatctttg ataattagat
     3481 ataatttttg aataacgttc cctgctattc gagctagaaa tacaagtacg ccatgaccta
     3541 gttagcgaca actacttaga gatttctttt gttctgggac aacaaaatgc catgacaata
     3601 gcagtaaata atgatacttc cgtaaggctc gccgtaaact ggggagaggt gaaattccta
     3661 tgtgcttacc caaagcggtt caaaaatttt aaactattat tttaggagat gattttataa
     3721 aatcgaaagc ctacttgaat aaaaaacaag cctgtgaatt tctaggaatt gataataata
     3781 ctttagataa gtggattcgt gtatataatt ttccagtaat aaaaattgat ggcgtagttc
     3841 gctttgacgt agaggatatc aaagatttca tgaatacatt caaaaaaaat tgaaattttc
     3901 ttctcgtcac atatactata aatagcgaaa tgtactgcct tactttttaa aggagtgagg
     3961 aagtttgcct acaaagcttt caaatgggaa atataaaact aatctgaggt atccaaaaaa
     4021 attcagagaa atcactggaa ttgcatcaga gaagtatcaa aaagtatttc caactaggca
     4081 attagctatt aaggccgaaa gggaaatgaa aaagaagatt gaaacagttt taagagagga
     4141 gaatgcaaac tcactagaat tgaaaggaaa aataaaattt aaagattttt atgaatcaaa
     4201 atggcttcct agatatgaac taggtcaaac tactaggtca aacagggctc caagtaatgt
     4261 aacaattagt aatactaagg atatattccg attgcacatc cttccaatgt ttggtgaata
     4321 tgcaatgaat tatctgaatt caaatactga gataatatca gatgaattaa caaagaaatc
     4381 gaaagaatac gcaaatataa aaattatcaa aggctatgtt cgctctatgt ttgatattgc
     4441 cgaaatacta tgttatatcg aattcaaccg aactaccaaa attattcaat gtattaccgc
     4501 gcctaagaaa atggctttag aagaaaaaag gataagggaa gggaatcaag cattatcatc
     4561 agaagagctg actgattgga tagaagcagt aaacaatgac ttaaataatc acttgcttac
     4621 acttcacgat tacacacttt tcatgcttac cttatatcta ggtgatagaa aaagtgagac
     4681 ttatgctctg caatggaaat atattgattt taataaaaat acggttcgtc tcaaatatgc
     4741 tttagataaa tatcaaagaa agacacatac aaaaggttgg aaagacaccg ttattcaggc
     4801 accagaagtt gtgatgacat tattaagaga atggaaatca gctcaggctg aacaattgtt
     4861 gaaactaaaa attatacaaa cacctgacca gtacctcttt acctattcca aaccttctgg
     4921 agaagtaaat tgtcctgttc acgctgacta cttaaactat cgaatcaatg caataaaaaa
     4981 aggcatcctg aattggctca attatctcca cacaaacttc gtcataccta tgcaactata
     5041 gctagggaag gtggagcaaa tatgaatcaa atctctaatg ctttaacgca ctcggatatt
     5101 tctactacaa aaatatacgt gaacacacct gacgtagttg ataaatctgt ttttgaagca
     5161 tttcaaaaag gattgaataa gtgtgattaa catgtgttct caaatgtgtt cttattataa
     5221 aaaaagacac tttccaaata attgaaaagt gtcctgaaat attgatataa aaagaatatt
     5281 aacgttttga gaattgtgaa gctttacgag cttttttaag acctggtttt ttacgttcga
     5341 ccatacgtgc gtcacgagta agtaagccag cacgtttaag agctaaacgg aagtcaggat
     5401 caacttctag caatgcacga gcgataccat gacggatagc tcctgattgt ccagcgtatc
     5461 ctccgccgtt tacgttaaca aatacgtcat atgcgccttt tgtttcagta acagcgaaag
     5521 gttgattgat tacttcacgc aagtcagcat gtggaatgta ttcttcaaca tcttttttat
     5581 ttacagtaat tttgccagtt cctggtacta gacgtacgcg agcaactgcg tttttacgac
     5641 ggcctgtgcc gatatattga acttgtgcca atgaaattcc ctcctattag attaagtttg
     5701 tgatgtctaa aacttctggt tgttgtgctg catgtggatg ttctgctcca ccgtatacgt
     5761 tcaattttgt aaattgtttg cggcctaaag tgtttttagg aagcatacct tttacagaag
     5821 tttcgatcaa acgacgagaa tttttagcac gcaattcgcc agcagtgatt gatttcaatc
     5881 ctcctgggta gttgctgtga cggtaataaa ttttgtcagt cgctttttta cctgttaatt
     5941 tcactttgtc tgcattgatg acgatgacga aatcgccagt atccaaatga ggtgtgaaag
     6001 ttggtttatt tttaccacgt agtacagatg caacaactgt tgaaagacgt cccaatggaa
     6061 catcagttgc gtctactaca taccatttac gttctacttc gcctggtttg gccatatatg
     6121 ttgtacgcac gttattttcc tccaatttaa gttctgatgt ttgacataca cttcgagttt
     6181 ccggggctct ttgtggggca aacaatacca tttaacatat tacccgctaa tctagtcaat
     6241 gtcaacgttt ttctgcttga ttgaaaaatt tatatgaaac tttacttctt ttctttcttt
     6301 tatataaaag ggtcgtttcc tgtcaaaatg aaaaacaaaa agaggctgtg acaaaagtct
     6361 tgtcacaacc ccttattttg aataaacggt gttctcctgc caactcctcg gtcacaagtc
     6421 acaatatcca taaaacataa gaagctgttt tttaaaattg tttcttgtga ctcggagctg
     6481 aacggcaggc ttacaacctc ttggtaaacg gtgttcaatc agctgaccct cggtattagc
     6541 tcaaaaaacg gcaaaatatg aggaactatt ttgacgtttg tttctctaat actcaggtca
     6601 aaacgctgat ttcacaacct ctttttcggt caagttcaac aacagttttt acgcgtcgat
     6661 ctctggcgct ttgcccaaat gagcttggat catccagata cgtttttctg tggctgtttt
     6721 gaattcgata aagatatctt gtgttggatc gtcgccttct tctccagaaa cttcgattcc
     6781 tttttggtaa agatctgcta gataacgata gcctgcaaca agtgtttcca aacgttctgg
     6841 aatagtacga tcccagcggc caatttcatc agggattttt gtgttatctg caaattcacg
     6901 caatgtagaa taaggtgaac cgtctaatgt gatcaagcgt tcggatactt catctaactg
     6961 agcgttcaaa tcatccatga actcatccat cattgggtgc aatgtaagga aacctggtcc
     7021 tctcatgtac caatgtgttt ggtgaacaac aacagacatt tggcttagat ctgcaactaa
     7081 ttgatttaat acttctttgg tttgttggaa tttcatcttt cttgcctcct aacattttct
     7141 tacacttaaa ataatagtag actttcaatg aaaagtacaa caaaatcatt tacctgagaa
     7201 cgattttcag ttcgcttttt tatccgtaaa gccaatcatt tttcagaatc agctaattct
     7261 tctcgcagtt gagcaaattg ctgataagtc tgtgctaaat gcttttgtgt cgaaacaaat
     7321 aatggataga ttttctgata ttttttatga tcagtttctt gtactccaac ttgctctttc
     7381 aattctatca attcctgtgc ttcactgata ctttggatct ttccgatgct tctccagcct
     7441 aaaatcacag ctcctaatgc ggatgcttct gtgacagtag ggaaaacaag cgggtaaccc
     7501 agtacatcag ccatcatctg acgaaaaatc ggagattgag caaacccgcc tgtagcacgg
     7561 atctctgtga gctgattttc ctggcaaact ccttttaaaa tccgataaag gttgagacag
     7621 atcccttcta gaatcgcctt catcatttct ttttctgtat gttgccgctg taaaccgata
     7681 aaactgccaa acgcatctgc gtcccaaagc ggtgctcgct caccaagtaa atagggatga
     7741 aaaatcagac cattggcacc cgctggcaca aaagcgatag ccgaaagcag tgcatcatat
     7801 ggattttctc ctttttcttt agcagattga acgacttcct gtaaaaatgt atgccctgcc
     7861 caatcaaaga tgccagcacc attgctagtc gctcctccca ctacccaatg tttctgatcc
     7921 agaacataac aaaacgtctc catttctgga tggaactgag gctgagtagt aataaaacga
     7981 agtgctccgc tcgttcctac ggtcaaagcg gcgatcgtat caccaaaagc acctaaccct
     8041 agattagaaa gcggcccgtc tgcccctccc aaaacaaatg atatatcatt tggcaaacct
     8101 agttctgctg ctgtttttga tggtagacct gttacttgat aggtggaagg aaagacttgc
     8161 ggcaattgct ctattcgaat gttcaagtag tcaagcactt cttctgccca ttggaattca
     8221 tggatatcaa aatatccagt tcctgaagct gttccgtagt cgctgatcag ttcgccaaac
     8281 aatctataaa agatatagtc tttgataccg acaaaataag cagttttttt gaataattca
     8341 ggttggctgt tttgcagcca cttgattttg ttcaatggtg tcattggatg aatcggcgtc
     8401 ccagtctgcc gataaaattc tttgccaagc acggtatatt tcagtttttc tgcttcgtcc
     8461 gctgctcggt tatctgccca agtgatcatt cgagataacg gtgttttctc ttcatccaac
     8521 aatatcaggc catgcatcgc agaggagaag gagacaaatt gaatgattgt gtctgaaggc
     8581 aactgtttaa ttacttcttt tataacggtg cagacacctt gaaagatcaa ttcctgatct
     8641 tgttctgcca tcccttgtgt ttcttggatc aaagggtagc gaaaataagc ggatgctgct
     8701 tcttgggctt catctgtgaa agcaactgct ttggtttgtg tggttccgat atctaatccg
     8761 attgaaacgt tcattttttc acctcaaatt ttatgtatgt acaaaaattt agtttaagct
     8821 tgcgtatctt ggttttgaaa agggggagat ggcgtgttac tttattttat cattggttgc
     8881 tgtatcggat catttttatg tttaaccgcc cagcggattc cgttaggtca ctctattatt
     8941 tatcctagat ctcattgtgt caaatgctgt cgttcgcttt cttggtatga attgattccg
     9001 attctctcga tcatcgtcca acgatttcgc tgccgctatt gcaggtgtcg gttgcccttc
     9061 tactatttgt tggcagaagc cttatgtggt ggtctttttg cctggttctt tactttttct
     9121 tccgcaagaa atttttcgac gttcatatgg atgctttcgg ctttgctatt ttcgttgatg
     9181 gatttatttt acttcatggt ccattctcac acgctattct gttcttgggc aattttatgg
     9241 attttttggt tgcaggccgg cgtcttccaa tggcaaagtg tgctgttaac gtttatcgta
     9301 ggcggtgtgt gccttcgcta tggtcaatcc tatctcggag ctggcgatac tctgttgatg
     9361 ctttcttgga gtggtggctt gtctctagaa gagttgcttc agatcttgtt tcttgctagt
     9421 ctgtttggaa tatccgtttt ttctgtttac tttatctgtc ataaaaaaaa gttggaaaag
     9481 ctgcctttca ttcctttttt gagcagtgcg ttgctgattg tccttcatct taggtattga
     9541 aaaaaggttg tgacaaacga cttgtcacaa cctctgatat atagtgttct cttaccaaat
     9601 ccttgtgact tggagctagg cgacaggttc acaacctctc cactaaatgg tgttcaatca
     9661 gctgaccctc agtattagct caaaatgctg gttttacaac ctctgatata cggtgttcca
     9721 taagtagctt tttcggcttc ttatttcttg acgccccaat gtcgacgaag gaaacctgca
     9781 cgtccagaac cttctcgata aagattgtaa tggagcttgt ttttcttgta ataatcctga
     9841 tggtaatctt ctgcaggata aaattcgctt cttggttcga tttttgtcac gattggctta
     9901 tcgaatcgcc cgctgtcttg caatgcttgt tttgaacgct ctgcaatatt tcgttcttct
     9961 tcatcattat agtagatcac tggacgataa gaatcaccgc gatcagcgaa ctgtcctaat
    10021 gcgtcagtcg gatcggtttg ctgccaatag atttccacca actgatcata agaaataact
    10081 tctggatcat attcgatctc tactgcttct gtatgccctg ttgtgcctgt tgttacttgt
    10141 tcataagttg gatttggtac atgcccgcca gtataaccgg atgtcacaga aatgatccct
    10201 ggctgctctt caaatggttt gaccatacac caaaagcatc cgcctgcgaa aactgctttt
    10261 tttgtcatat aaaatccccc ttttttctca tcataccttg atttccaaaa ggaggataac
    10321 gttttgcttg tgacagaaaa aacggaaact attatatacg tgatacggct tctgataaat
    10381 gccaaaacga acgtacttga tcaaaaacgg ttaaatcagg aatcacatat tcagcagaag
    10441 gaagatttat gttttcacct attcccaata catgcatgtt accagccaaa gctgcttgac
    10501 aaccagcttc tgaatcttca atgaccaagc atgcatttgg cggtacattc aattgctgtg
    10561 ctcctttgag aaaaacttct ggatcaggtt ttgcttttga tacttgcgtg ccgtcaataa
    10621 ggacatcaaa ataacttgtc aaattcgttt tttctaaaat gaggcgtgca tttttgcttg
    10681 ctgatcccaa tccgatcttg atgtttgttt ttttcaagta ctcaagaaca gctattgcac
    10741 caggcaaaac ggatgtttca tccatttttt gaatcgcttg aagatataga ttgttttttt
    10801 gctcagcaaa ggctgatttc tctttttctg agaatttctg ttccattttt ccaaatgaca
    10861 gcaatcgctc tagtgactcg gttctggata ttcctttcag ttgctcgttt tctttctcag
    10921 tgaatggaat ggacagctgg ttagctaaaa ccagccacgc ttcgtaatga taacgagctg
    10981 tgtctacaag aacgccatct aaatcgaata gtgctgcttt gattttcatc gattacctcc
    11041 ttgaaaatga acgacttgaa gtaaagatgc gatgttttta gctgtgcgat gaagattatc
    11101 agtggcttgc tgtaaagctt cttgtaaagg cacagctgca ggaaggatcg gaaaaacggc
    11161 cgaaatgcca acatctgctg ctttttcgct tcctttacca acacttccgc aaatagctat
    11221 cacaggaacg ccctctggta ctcttctagc aatcccaact ggcgcttttc cttttagact
    11281 ttgtccatct agacgtcctt cgcctacgat tacgaggtct gcttgctgta cccgtatcga
    11341 actccagtaa atccaatacg tagtcgatgc cagagactat tttccctgaa gcaaatgcta
    11401 acagtcctgc acccattcca ccaccagcac tactgcccga tgcctcgatt acttgagggg
    11461 caaatctcat ataaaaatca tgcatggctt ggtcagccac tgctaattca ttttctttca
    11521 agcctttttg tggtccgaaa acaaaagaag cgccttcttt tccgcataaa agattagtaa
    11581 catcagtaat gacagtgatt ttggctttaa tcagctcaat aggcacgttc gttttatcaa
    11641 ctgtagcaat tttacctaga ttttctccca gtgcttctac tttttgatta tcttgatcgt
    11701 aaaaagcata acctagacct tctgccatgc cgatgccgcc atcgttggta ctacttccgc
    11761 caacgccgat gatgatgtgt cgaacacctt gttgcaacaa ttgtcgtatc agttctccta
    11821 ctcctcgcgt ggataaccta agtggctgtt gttggtttag tgggacatct ttcaatccga
    11881 cgatttctgc catttcaaaa atcgcagttt gaccgtctga agcgtactgg atttctttgt
    11941 ttttgccaaa tgcattagta acgactgctg tttgccgctt caaccttaaa ctgtctgcta
    12001 atgatgaaac cgttccttct cctccatcgc ctataggtag cagatcatat tcagcctgaa
    12061 caaaaacttc ttgaaaacct gattgaatgg cttgtgctgc ctctaaggct gtcagacttt
    12121 ctttgaaaga atctggcgca atcagaatac gcattatttt cccctccgtt ccaagatcag
    12181 ttctttttcc agctggtatt ggtctccatt gacaaaaata ggtaatgttt ctttcgtctc
    12241 tttttttatt actatttgtt tttttgtcaa aacaaatagc aacctgtgat ttttccaaat
    12301 aaaaggaaaa gaaatttttt cccatgctga aggaagtctt ggagaaatag aaagagttcc
    12361 attcgttact gtaatacctg aaaaaccaaa tataactgct agccatagtg ctcctaatga
    12421 agcagcatga attccgtcat ctgatgaatg cggatctggt cctaaatcaa tcaggcacgc
    12481 ttcttggaat aaatgatacg cccattccat ttcttggata cggcaagcct ctatggcgtg
    12541 aatcgcttta ctcaacgatg agtcgtggat tgttttgttt tcatagtaat gcaaattctt
    12601 agaaacgatt ggttgagaaa aatactcagg taataagtat aacagcatga caagatctgc
    12661 ttgcttgctg acttggagtt catttacttc gctacgggca taatcaagta aaatagactg
    12721 gctgccagga cgttctttgt aagtggtcaa atcaataact ggcttactga gatacgtgtc
    12781 atcttgcgga atcagctgtt ctttgttagg cactggcaaa tataacttat ctaaaaaatt
    12841 ttgacattct ttcaaaaaaa gcggatcgcc agcttgatat ttaacagcta aggccacatt
    12901 ataatgcgcc agatagttcg tatatgcatt attgtcgata tattctgtgt attcatctgg
    12961 accaatgact ctgtgtatct ctaaatgatc atcgactttt acagctctgc tcagccaaaa
    13021 agctgctgtt tcatgaatga gcgtttttcc ctttgtttct aaaaacgatt gatcctctgt
    13081 tgcttggaca tactcttgaa cggcataagc aaggtctgca ctgatatgat gttctgcggc
    13141 agctgaagca acttcttgtc ttgttccggt tttgatattc aatgcagcaa atttgggtgt
    13201 ctcttcctga ccagtgtgtg cgctttccca tgggaaaagt gcaccttgat agtgataatc
    13261 tcttgctttt tgccgtgcgc cttctaatcg atcataacgg tacagcagca aattctttgc
    13321 tacttcagga aactgatata aaaagaaagg caggataaaa atttccgtat cccagaaaac
    13381 atgcccttta taaccttcgc cagttagtcc cttcgcccct acactaacac ttggatcatt
    13441 gcggggagtc ataatctgta aatggtaagc agcgaagtcc atcgctaact gatcgaaaga
    13501 agaatcgcct tggatcgtta tccgatgctg atgccaaaaa tctgtccaat aagacatcga
    13561 ctgctctaaa atttctgtat aactaacgct tttctgggct tgggcaatac ttttcttaaa
    13621 cgggtctgtt tgctgaagag aggtagagag cacgatgatc ttctccagcg taaaggacgt
    13681 attttttctg atcttttctg aaaaggaagc ggtgatccgg cgattttttg cttggaatac
    13741 acctgcttta ttgaatttag ctgcaatccc aatatcaatt ttactttctg ttgtctggta
    13801 ggatgcactg atcaaatcgt caccaaatac tcgcagttct ttttcaacca actgctgtgt
    13861 accaaaattg gtttgctgtc catcgatacc cgttttcaca tctacttggc agtcttcggt
    13921 caacggcttg atgaccactt tcagggctgc ctgatggaga ttattttgat cagcaacacg
    13981 acaagtaatg acttccactt tttttccttg agcagtttca atgagcgcat agcgtttaag
    14041 ctctcccatt tctaaatcta gtgcccgctc gtatgcaaga actttcatcg tctcaagaga
    14101 aaaatgttca ccgtcgattt ttatttccca tctgatgaca tctgggagat tcactaactc
    14161 ggctgattct gctcctaaag gtcggttata tacaccagct gcaaacattc cccttacttg
    14221 attccgatag tcttcctcct ggcttcctct gataccaaaa aatccatttc caatggtcat
    14281 ctgactcgca tacttattga tatactctaa atcaaactgt ttttcttcta ctttcataag
    14341 gtaataactt ctcctgtttc tgccgaacga tataaatttt ccaccagatt ctggatgaca
    14401 tatccttgcc agccatctgc aatcatctga gtctggtcac cgagcgcccg attcacaaaa
    14461 tgagccatac ttagttcatg tgggtcatta actggcgtgg tcttttgtaa ggaaaccagc
    14521 tttccatcta catcttcgaa tagttcgcct ggatacagat tagcacctgc ctgatcgcca
    14581 caaaaagcca cattcagttt gtcttctctg atattcaaag caaacgaagt attcagttgg
    14641 atgatcttgc cgttttctaa ttccagtgtg ccaaaaattg catcttctac tgtgaatttg
    14701 cttggatccc atttcccgaa tttccctgaa ttttttttcg taccaatttt ttggaagctg
    14761 tacgcccgaa cgctttttac tttaggaaat ccagtaatat acatcgctgt atcaagcatg
    14821 tgaatgccat aatcgatcaa aggcccgcct ccttgaagtt ctttgtcgat aaacgttccc
    14881 cagcctggaa caccagaccg acgaagcgca ttcgcttcta cgtaataaat atccccgaga
    14941 acatcaagat gattcttgat cattgttgct tcgtcagaaa aacgatgctg gaatccataa
    15001 gccaaaatct gcccgttttt atcggctgtc tctgccatct ttcttgcttg atcagctgtc
    15061 atagctggtg gtttctcgca catcactgaa gcattagctt cgagggcagc caatacatgt
    15121 tcgaagtgaa agcgattggc tgtacagaca ctcacgatat cgggagaaat ctcattcagc
    15181 atttgtgtaa ctgatgtata cgcatgagaa aatcctgtac gtcggcaaaa gtcttctgct
    15241 cgctggctga ctggatcaac cacagcggcc aattctaaga catctgatct tttttgataa
    15301 taagcagcat gtactttttc tgctacttgt ccagcgccta tgattgctac tttcttcatt
    15361 tatttctctc tcctttgctt atttacttat tgttattctt tctctattct ttctctaaat
    15421 catttaaatg acgcgctgtt tccagacagt ttttcaagta ttgcaatgat tcctgataaa
    15481 ttttcacttc attttctcct cgcaaacgac attcatatac catataacct tcatactcat
    15541 tttgaagtag cgtttgaaaa tggcgagtaa aatcaattgc gccagatcct ggttggtagc
    15601 gatgattatc tgctaaatgg atatgaccca catactcttt ttgcgtagtc aatgcttttg
    15661 aaatatcatc ttcttcaata ttcatatgat aaaaatcagc gatgatctgc acatgtttca
    15721 attgattaga gtcaatgtaa tgacgcgcat ccgctaacaa attgatcata tggtcttggt
    15781 atcggttcaa aggctccaaa taaataaccg tacctgtttc ttcggctact ttatctaaat
    15841 aacgaagtga ttcgctgact gctttatagt ctccttcttt gcttcggggt gaaaccatcg
    15901 gcggtaatcg ataagtgaac attccccaag ctgctgggat aacgattcct ttgcctccaa
    15961 cctcgcttag tgcacgcaaa atctcagcaa tttgacgtac accattaaga cgccgttctt
    16021 cgataaagtc accgatccac ccatcgtagc cgttacaagc tgtcactact gggatccctg
    16081 tttctttgat tgcctgtttg actgtgtcga tattttctat taacaactta ccgtcgattt
    16141 cgtattcttc aaatccacag tctttgataa atctaaattt ttctaaaatg tctcttggga
    16201 aaaatgcttg atcttgcgta ccgaatttca ttgttctcct cctcggactt gaacgcccat
    16261 tttgatactt tcttctgggt gctggtctac ataacgcatg taagcttctg cgctttctgc
    16321 aaatggcaca actggatcaa tgatttcttc acaatccaag taaccgttca tcaataattc
    16381 ccaacatgtg tcctcgattc gtttacggtt ccatcttggg taatctgggt ttggttcact
    16441 agatgctcga gcaaagacga tttttgcatt attgaaatgt gcttcgcgac caagattaaa
    16501 cccagctgga aaaggtttag caaaggcaac ataagaaatg atcccgccat agccgatccc
    16561 tcttaaagaa gcttgtaatg cggcagcact gccactcgtt tcaataactg aatctactcc
    16621 acgtttatct gtcaattcct ttagacgaaa accgacatct gctttcatag gatctagcgt
    16681 gtaatcggca ccgtattttt ctgctaattc tctacgctgc gctatgggat cgatcccaac
    16741 gatggtagta gcacctgctt ttttagctaa ttgaatcgca atttgcccga tagcgcctaa
    16801 accgaagatt gctacagcat cacctgctcg aacatgtgct tcccggatac cacctaaagc
    16861 gaattgagca ggatcgtaac atactgcatt tttccattga tccaaccgat caagtttacg
    16921 tagtttataa ttatcaacac cgtttgtaat cagcgtttca cgaatgggtc cataagtgca
    16981 gactaactct cctttttgat actctgtcac caaactgccc gtttcttcga tcgtaccgac
    17041 aatcatattt cctaattgaa attcgccaaa taccacaccg cgttggcttc cttcttctct
    17101 tggcaagaat aggcgccatt cttctgaaaa ttcttcatcg ataaatggac ttaatcctct
    17161 aaagtcgaca atttctgttc ctggttttgg agtagcgaaa attactcgga tacgtacttc
    17221 gttcttttcg atctcacggt cttcgtaatc tttgaatccg gctattcttg gttctaatgc
    17281 aactaatttt ttcatctttt ctttcctctt tcttttcttt ttatcctttt acgccgcctt
    17341 cgactaaatc acctttaata aatcgttcag ataacgcata cataatgatg actggtaaag
    17401 cggtcaccaa tgaagcggcc atcattcgtc cccaaatgta gtctggtgta ctgaataatg
    17461 tgttcaaacc gattggtaaa gtaaattttt cggaggtcga caggaaaatc gaagcaaata
    17521 aataatcatt ccacgaaacc ataaagcaat aaacgaagac agaaatgatt ccagaaatag
    17581 ccaatggtat aatgatccga ataatgatct ggatccggct aagaccatcg atcattgctg
    17641 cttcttcaat atcagtcgga atcgtgtcaa aataactttt tagcatgaat atcgcagtag
    17701 gaagtgtttg aacgaccatc gtgataatca gtgctgtatt ggtatcatac aaacctagac
    17761 cggaaatgat tttaaataat ggaacaacga tcaaaatacc ggaaaacata taaacggtgt
    17821 aaaagctagc attgattttc gagcgagctt tgaagcgtag ttttgacaaa gcgtatgccc
    17881 cgctgatccc aatcactact gcagttacgg aggctatcaa ggagatcact aaactatttt
    17941 tgaaatacat gatgaatgga aaaatctcag cattgaaaat atcctgataa tgttcaaaag
    18001 tccattcttt cggaaaaata gtttgtgtcg ttgcaattgc ttcttgtgaa tccttgaatg
    18061 atgtcatcag catgatcaaa aatggaaaca aattgacagc taatagcaac aaaatcgtcg
    18121 cgtaaaataa gattttcctg ctctttttat ttttcatttc aagtccacct tctttcgtga
    18181 gagcaagacg accgctgcaa taatgatgaa caatagaatc gatatagctg ctgcttttcc
    18241 aagatcattg aacgcaaagg ctgtctgata caaataaacg cctaagatat tgactttatt
    18301 cgtcagtaga tagacatctg taaacatata gaacatccag atagatcgta aaacaatcac
    18361 agttgctagt actggtttga ttgccggcag tgtcacgatc ttgaacttct gccaagcatt
    18421 tgcaccgtcc atttcagctg cttcataaag cgttttgtcg actgtttgca aaatagccaa
    18481 aaatgaaata aacgcatacg gatagtatct ccagatagca aacaaaacga ccataaagaa
    18541 gctagcaact ggctggtcga accacagcgg tgcttcgttg aaaagctgca acctatctac
    18601 taacagatca ttgacgattc cataactatt attgaacata tatttccaag caaaaatcaa
    18661 agaaatagat ggcgtaacat aacttaataa aatcagtgaa cgcaccgttt tcctaaatct
    18721 gaacttgcgg ttaagcaaaa tagctacggc taatcctaaa gctgtactgc ctgctacaac
    18781 taagaatgtg taagcaactg ttaaacctaa agaacgataa aattcactat cacttaaaat
    18841 ttcaagataa ttgcttaatc cgataaaaac ggcgtctgag ttaggattca gcggcatatc
    18901 gaaaaaactg atctgaatat tggaaagcat tggaatcagc actaaaacag ctaataaaag
    18961 catgcttggt gctaaaagtc ccatggctaa ttttaagtca gaatgtttcc ttggacgatt
    19021 cattgaaacc tccccctgat aatctgccaa tagctatcta cttttttcga acaccattgg
    19081 cagaacttct tgttatttcg tatttttttg tgcgtcagct aaagcttgat cgacatcttc
    19141 gtttccgaca gttacttggt tcactgcttt tccgatcaca cctgagcttg tgatatctcc
    19201 cattttcgtg aaattctttt ccccgactag tccaaagact tgtacttcat caaaagcact
    19261 agcaatctca gatggcagct cgccaaaaga cttgatgact tcattttctt tgtaaccatc
    19321 cagttcgact acttgtttat tcactggttg agcaccacca ggagacatca agacccactt
    19381 ttccatgttt ttcggttgag ataagaaagc tgtgaatgct tcagcagcct ctttttgttt
    19441 ctcgtctaga ccagaagaaa tcgtcaatcc agatactgtg ccatagacag cttgatcttt
    19501 ttgtgttggg atagcaaaac cgatattttc tgagttcccc gcttcataaa cagaaggcaa
    19561 aatgtaagtt gaatagattg ccattggtgc tgttccattc atgaaagcat ctttgatttc
    19621 tgtcacatca ttagagcctg gcatcgtgta ttgggataag ttttgataaa acgacaatgc
    19681 ttctctcatc ttttccgtat cgatcgttac ctcgccttca tcatccaaaa catttgctcc
    19741 atttgacaaa gcaaattgag agaacgcttg ctcactcatt gttccttcag cagtaggcat
    19801 agcaattcca tattgtttat tctctttatc tgtgaaatgt tgagcaattt ttaaaatatc
    19861 atcccaattt tctggttctg aaaaaccagc ttctgataat ttttctttgt cataccaaat
    19921 accttgaacc cagccgctaa ttggtgcagc aatatagctt tttccatctt ctgaacgaac
    19981 aaggtttttg gctccgtcat aataattatc ttctccagct tcctcgataa cgttttggac
    20041 tgccgtctga tcaatcagtt catctttatc catcacttta gcaaaatcct gactgacttc
    20101 cattacttct ggtaatttac cggaacgagc taacgtaacg atttttgtat tgaacgctga
    20161 ttcttctacc ggcacttgct tgattttgat gtctggattt tctttttcaa aatcagcaac
    20221 cagttcattg atcacactta atcgttcctg ttcgacagaa gagtgcatga attgaatcgt
    20281 tgtctgttgt gattcgttag agctttgact ttcaccacaa gcagtaagaa gaaatccagt
    20341 agctagcaga caggtagtaa caataatttt tttcattttt ttccacctct tattttatta
    20401 ttttcagcca catatattca tacggctgga atatcaactt tcgttcttga ctgcaatgga
    20461 taatttgtcc agaaaacagg tctaggtaat ctccttttgg taaaagataa cctgctgatt
    20521 gattggaaaa attatggata actaagactt tctcttcctt cgcttcgcga ataaaactga
    20581 taaaagaatc tgctgagtcc agtaagtaaa tcaaactatt cggatggaag gctttttctt
    20641 tttttcgaat agatagcatc tgggtcaatt caccatatac tgctgttctt aatggatctg
    20701 tctgcaactc tttctcaata tctgctaaca aaaatttttg ccgattgatt tttcgattct
    20761 ctcctgtttt ttctacgcct ttgtaatcat ttcgacttcc cagcacactt tgaatataaa
    20821 ttgccggcac gcctggtaac gttaccaaaa atgaatgagc agctaaaaag cgtttgcttc
    20881 tccattccgt cgtatctgtt ggagaactta acgcatctag atacgtagcg tttatctcat
    20941 atggactggt cgttccatct gggtttttct tgtagtttac tcgggcaccc tctttcttca
    21001 gctgttccac taattcaagg atcgtttttt ccggcagaat acctcgtacc ggattcaatc
    21061 cgataccgtc atgggaggct aaaaaattaa aaaaagttgt ttttttcgat gcgaaatcga
    21121 tggtttttaa ccattctttc aacttacttg tattacccga acgaatcgct tgaagggtca
    21181 aaggtggtag cgaaaactga tagaccatct gtgcttcatt ctcgccattc ccaaaataac
    21241 tgatattatc tttgtgtggc acatttgtct cagtgatcaa taccgttcct ggagctgctt
    21301 catcagcgat tgcacggaat aattggatga ttttatgcgt ttctggcagg tgtatacaag
    21361 aagttcctag ttctttccat aaaaatccca cagcgtccaa gcgaataaat gaagcgcctt
    21421 tttctaagta aaacaaaaga atatcgatca ttttcaataa aacttctgga tttttaaaat
    21481 tcaagtctac ttgatcttcg ctaaaagtcg tccatacgtg tttttctttc ccgtttttgt
    21541 caatgaacgt tgtaagtaac gggctggtcc ttggtctagt cacgctgctt gtatctgtat
    21601 ccagtggcaa atcaataaaa aaatctttgt acgtatcatt caagtttaaa tattcctgaa
    21661 accaagtact ttcggcagac atatgattgc agacaaagtc gaacattaac cgtgtttcgc
    21721 ttctcatttc ttctatatct tcccatgtgc ccaaacggtc gttgattgcc tgataattta
    21781 caacggagaa accatcatca gatgtatatg ggaaaaatgg caagaaatgt acaactctaa
    21841 atgtatctgc caaatacttt tgataaaatt tcttaaatgt acgcaaagtt ggttgctgct
    21901 cttcaaaaaa ttgatcccca taagtaatca agaaaatatc ttcttcgtcc caatgcagtt
    21961 tgcctgcctg gcctttcttc tgctgataaa cactgacttt ttgttcaatc atagaggcgg
    22021 ttttctcaga caaatctgag tcgcgataaa tcgcttgtaa aagttttgta tacatcggat
    22081 tcccctttct tttttgggag cgttcccaac gatttgatca tattatggaa aacgcttact
    22141 gtcaatagca tttttttaaa taattttgat atacacaaaa atacaaataa gaaagcttat
    22201 gtatcaataa ttaatagctt cttaaaaaaa tatggtctct catgatcatg caagctattc
    22261 ctaaattttc aaaatcatag acaaaaaaga atgtctattg tactatggaa ttattctaaa
    22321 acgctcccaa aaaggaggtg catataaaat tgaaacctac tatttatgac attgcgaaaa
    22381 aagctaatgt ctcaaaatca actgtctcca gagtattaaa taaccagcca aatatctctg
    22441 ataaagcaaa agcaagtgta cttcaagcaa tcaaagaatt gaattatgaa ccgagcaaaa
    22501 ttgcaagagg actgtcagga aatggttttg acgcgattct agcattatct catcgtactt
    22561 ctcatacgac ctatggcaat cctttttttt cagatgttat tcaatccatt tctgccgttt
    22621 ctgaacaaaa taacttcgac ttgattctac aaacggcaaa aacaccagaa gaagagctag
    22681 aaaaatgcgc gaaaaagatc caagaaaaaa tgatccgagg aattattatt ttaagctcta
    22741 cgatggatga aacacttctt tttgaattgg atcaatacaa tattcctatt gtggtaatcg
    22801 gaaaagtcgg aaacgactac acgaacatct actcagtgga cacggataat ttccaggata
    22861 gttacgattt ggtggaaaca ctgatcaaga atggacaccg tcatatcggc tgtctccatg
    22921 ctccgacaga agtttatgta tccaatgatc gtctcaatgg ttaccgtcaa tgcttatttg
    22981 atcaccacct agatatccgt aatgaatgga ttatagacgg cggctttcgc ttagaagata
    23041 gcatggcagc aatgaatgat ttaatgaagc tacgagaaag accaactgcg attttttcta
    23101 ccgatgcatt aaaatcattg agtatttaca aagtggcaga acaatctgga aagcagatac
    23161 ctgatgatct ttcgatcgca ggattcagtg atcccttatt gtcctcccta ttttcgccac
    23221 agttgactcg gatagacatt ccgacaaatc agttaggaat caaagccaca gaacttttat
    23281 tccaactaat ccaccaccaa taccctgaag aaaaaagaat cattctttca accgagatta
    23341 ctctcacaga ttcgatccaa caacaagaaa tgtagaacag aattataaaa gaacagaaac
    23401 actgaggata agtcagttac tctgttcttt tttcaatcac tcatccctcg ttaccccctc
    23461 ccggtatttt agcgaatttt tcttattttc catctgtttt agatagctgt ggataactat
    23521 aatgtttgtg ttaaactatt ttttccacag ggaagaaaaa actattttta accacgttgt
    23581 gtataagttt ttatagttcg tttttttaaa cacatttacc cttatttcaa tccatgttat
    23641 ttggggatac ttatctctaa aacataaata gaataacaga catgtctact ggttttgttt
    23701 tattcgagga attattcaat aatagctagc aaaatattaa aaataaccgc tctatcttcc
    23761 catcaattta ttttgcattc aaaaaatgat ctatctttac ttttgttgat tgacttctct
    23821 tggaagatga tcgttccgat accttaattg gatacgaacc gatgttgtta agatcctttc
    23881 ccacttttct tctagttaga caaattattt tagacaaacc aaaaaagagg ttgtgacaat
    23941 aatttgtcac aacctctttt ttaaaatatc tattttgttt gattgttcat tgcgtcttct
    24001 gcagccattt gcgcttcaat atctgaaata ctgtagacat tttcgcttgc tacaccgact
    24061 tcttttggtt ccatgtcacg ataacgttgc ataccagtac cagctgggat gatcttacct
    24121 ataataacat tttctttcaa accaagaaga ggatctttct taccgcggat cgcagcatcc
    24181 gtcaagacac gagttgtttc ttggaatgat gcagcagata agaaactatt tgtttctaat
    24241 gatgcttttg tgatacctag taagacaggt cgagaagttg caggaacgcc tcctgagact
    24301 agcgtattgt agttttgatc tttaaagtct gcgatgtcta ataatgtacc tggtaagata
    24361 tctgtatcac ctggatccat tacacgaact ttacgcagca tttgacgaac cattacttcg
    24421 atatgcttgt cgccgatttc taccccttgc atacggtaaa cacgttgtac ttcacgaagc
    24481 aagtagtttt caacagataa tacatcgcga acttgcaaca attgttttgg atcgattgat
    24541 ccttctgtca atggtgcacc acggtggatc atatcgcctt ctgctacttt catacgtgct
    24601 gtataaggaa cagtatacgt acgtgtatct gtttttcctt tgattgttac ttctttggta
    24661 cgtgttgcag gatcttctga gatatcgatg acatcccctg ttacttctgt gatcactgct
    24721 tgccctttcg gattacgtgc ttcaaagatt tcttggacac gaggaagacc ttgagtgata
    24781 tcgtctccgg caaccccacc tgtatggaat gtacgcatcg tcaactgagt accaggttca
    24841 ccgattgatt gagcggcgat cgtaccaact gcttctccga cttcaacgtc agagccagtt
    24901 gcaaggttgc gtccgtaaca atgtttgcag acaccatgtt ttgtattaca tgtgaagaca
    24961 gaacggattg tcactgtttc gacacctgca tcaacgattt gttttgcgat gtcttcggaa
    25021 atcaattggt ttcctgcaat gatcaattca tttgtttcag gatgacgtac ttctttacgg
    25081 gtgtaacgtc ccactaaacg atcttctaat gattcgatga tttcatttcc ctcgcggatg
    25141 gcttggattt caagacctcg gtcagtaccg caatcttctt cacggatgat tacatcttgg
    25201 gccacgtcaa ccagacgacg agtcaagtaa cctgaatcgg cagtcttcaa ggctgtatcg
    25261 gtcatccctt tacgagcccc gtgagttgag ataaacattt ccaagacgga tagtccttca
    25321 cggaagttcg aaatgattgg caattccatg attcgaccat taggagcggc catcaaacca
    25381 cgcattccgg caagctgtgt aaagttggaa atgttaccac gagctccaga atcagacatc
    25441 atgaagattg gatttttcgc ttccaaacct tccatcagtt tttgttggat gctgtctttt
    25501 gcagcattcc aaacagcaat gacacgttca taacgttcgt catctgtgat cagaccacga
    25561 cggaattgtt ttgtaatagt ttcaacttgt ttatgggcat tttcaatgat ttcttgtttt
    25621 tcattcagta cactgatgtc agcgatccct actgtgatac cggcatgtgt cgaatgtttg
    25681 taccctaagt ctttcatacg gtcaagcatc ttagaagttt cttttacttt gaatcgtttg
    25741 aagacttctg cgatgatgtt tcctaagttt ttcttcttga acggtaatac taattcttgc
    25801 tctttgatat gtgcaggaat atcagtacca gcttcaacaa agtatttgtc cggtgtttgg
    25861 acggtcaagt tgaagtctgt tggttcattc agataaggga attcaggagg catgatctcg
    25921 ttgaagatga tcttaccaac tgttgtgatc atcgtacgtt ctttttgcca ttcagtaaat
    25981 ggtttttcac ctaatgagtt agggttcaca ccgatacgtg aatgtaaatg aacatatccg
    26041 ttgcgccatg cgataacggc ttcgttttga tctctgaaga ccattccttc gccttcgcgt
    26101 ccttcttctt ccattgtcaa gtagtagtta cctagaacca tatcttgaga aggagtaaca
    26161 actggtttac catctttagg gttcaagatg ttttgagcag ctaacatcaa catacgtgct
    26221 tctgcttgtg cttcttcatt caatggaacg tggacagcca tttggtctcc atcgaaatcg
    26281 gcattataag cttcacatac aagtggatgc aaacggattg cgcgtccttg tactaaaact
    26341 ggttcgaatg cttggatacc aagtcggtga agcgtaggtg cccggttcag caatactggg
    26401 tgttccttga ttacgtcttc taaaacatcc catacttcgt cttcttgacg ttcgatttta
    26461 cgtttcgcat ttttgatgtt tgaagctagt tcgcgttgaa caagctcacg catcacgaat
    26521 ggtttgaata attcaatggc catttctttt ggaagtccac attggtacat cttcaagaaa
    26581 ggtccaacaa cgataacgga acgacctgag tagtcgacac gtttacctag caagttttga
    26641 cggaaacgac cttgtttccc tttcaacata tgagaaagag atttcaatgg gcggttacct
    26701 ggtccagtaa ctggacgtcc gcgacgaccg ttatcgatca atgcatctac tgcttcttgc
    26761 agcatacgtt tttcattttg aacgatgatt ccaggtgcat tcaagtctaa caaacgtttc
    26821 aaacggttat tacggttgat tacacgacgg taaagatcat tcaagtcact tgttgcaaaa
    26881 cgtccacctt ctaattgaac cattgggcgt aaatctggtg gaataacagg gataacgtcc
    26941 ataaccatcc agcttggttg atttcctgaa gcgcggaatg cttctaaaat atccaaacgg
    27001 cggattgcac gtgtacgttt ttgtccttgt gcagatttca attcttcttt caattcggct
    27061 gcttctgcat ctagatcaac attatcaagt aattgtttaa tcgcttctgc acccatagca
    27121 gcttggaatt cttgtccata ttgttcacgt ttttcgcgat attcgcgttc tgtcaacaat
    27181 tgttttttct ctaaattcgt atttcctggt tcgatgacta catatgaagc aaaataaatg
    27241 atttcttcca aagcgcgtgg gctcatgtct aatacaagac ccatacgtga aggaatccct
    27301 ttaaagtacc agatatgtga aacaggagct gctaattcaa tatgtcccat gcgttcacgg
    27361 cgaactttcg aacgagtaac ttccactccg catcgatcac aaacgattcc tttataacgg
    27421 atacgtttgt attttccgca ggcacattcc cagtcttttg taggaccaaa aatccgctca
    27481 cagaacaacc cttcgcgttc aggttttaag gtacgatagt taatcgtctc aggttttttt
    27541 acttcaccgt aagaccagct tctgattttt tcgggagaag ccaaacctat ttgcatactt
    27601 tcgaatttat ttacatcgat caaaaggggt tccctccatt tcatttaatg gcaaaccgca
    27661 agacggctga tcttcgaccg ccttgcagct catagaccgg attagtctaa attatcttct
    27721 gcggtttcaa agttttggat gacatcgtct ttttcatctt ccacttgttc agcagctttc
    27781 ttttctaatt ctttggcagt ttgctgttcg gcaaattttg tcaaagcatc aacagtgatc
    27841 aaatcgtcgt cttcatcgtc catgtcgcga agttcgatct ctgtttcttc gatatccaag
    27901 acacgcatgt ccagacctag tgattgcaat tctttcacca atacgcggaa tgattccggt
    27961 acacctggtt ttggaattgg ttcacccttg acgattgctt cgtatgtttt cacacgtccg
    28021 actacgtcat ctgatttgta tgtcaagatt tcttgtaaag tataagccgc accgtaagct
    28081 tccagtgccc atacttccat ttcaccaaaa cgttgtccac caaattgagc tttacctccc
    28141 aatggttgtt gtgtaaccaa tgagtaaggt ccgattgaac gtgcatgcaa tttgtcatcg
    28201 accatgtgag caagtttgat catgtacatc acaccgacag acacacgtcc atcaaatggt
    28261 tcacctgtac gtccgtcgta cagaacagtt ttcgcatcgc tggccatacc ggcttcacga
    28321 actgtttccc atacgtcttc atcacttgca ccatcaaata ctggcgttgc tacgtggata
    28381 cctaactgac gtgcagccat acccaagtgt aattccaata cttgcccgat gttcatacgt
    28441 gaaggcaccc ctaaaggatt cagcatgata tcgattggtg ttccatcagg taggaatggc
    28501 atatcttctt ccggcataat acgggataca acccctttat ttccgtgacg accggccatt
    28561 ttatcccctt catggatttt acgtttttga acgatataaa cacgaactaa catgtttacg
    28621 cctggtgaca attcatctcc agcttcacga gtaaagattt tcacatcgtg aacgataccg
    28681 ccgccgccat gtggtacacg taatgatgta tcgcggactt cacgagcttt ttcgccaaag
    28741 atcgcatgca atagacgttc ttctgcagat aattctgtta ccccttttgg cgtgactttc
    28801 cctacaagca aatcgccatc ttggacttct gcaccaatac ggatgatacc catttcgtct
    28861 aaatctttta atgcgtcttc cccgacatta gggatttcgc gtgtgatttc ttcaggtcct
    28921 aattttgtat cgcgggcttc tgattcatat tcttcaatat ggatagaagt gtatacgtca
    28981 tcttttacca aacgacggct catgatgatc gcatcttcat agttgtaccc ttcccaagtc
    29041 atgaaaccaa ctaaaacgtt ttggcctaga gccatttcgc cttgttccat agatggtccg
    29101 tctgccagag tatccccttt ttcgactttc tcacccaaat gaacgattgg acgttggttg
    29161 tagcttgttc cggagtttga acgacggaat tttgttacat cgtatttatc taatgcaccg
    29221 ttgtcgcggc gtacgcggat ttcagaagca tcaacgtatt caacgacacc atcatgttta
    29281 cataacaaag cagctcctga gtcatgcgct gatttgtatt ccataccagt accaacccat
    29341 ggtgcttgag gattgatcaa cggcactgct tgacgctgca tgttggcacc catcaaggca
    29401 cggttggagt catcgttttc caagaaagga atacatgctg tcgcaacggc aactacttgt
    29461 ttaggtgata cgtccatgta gtctacttta tcaatagata cttctaagtt ttcactttga
    29521 gcacgagcca taacaacgtc ttgagcaaat gttccatctt cattcaatgg gctgtttgct
    29581 tgggctacga tgtaatggtc ttcgatatca gctgtcaagt agtcgatttg gtcagtaaca
    29641 cgacctgttt cacggtctac acgacgataa ggtgtttcga taaatccgaa tttatttact
    29701 ttcgcataac tggacaaact attgatcaac ccgatgtttg ggccttcagg agtttcgatc
    29761 ggacacatac ggccatagtg ggagtagtga acgtcacgaa cttcataacc ggcgcggtca
    29821 cgagtcaatc cgccaggtcc taaggcgaaa agacgacgtt tgtgtgtcaa ttcgccaaga
    29881 gggtttgttt gatccatgaa ctgtgacaac tgagaagaac caaagaattc tttgatactt
    29941 gccacaactg gacggatatt gatcaattgt tgaggtgtca atgtttcagt atcttggatc
    30001 gacattcttt cacgaaccac acgttccata cgtgctagcc cgatacggaa ttggttttgt
    30061 aaaagttcac cgactgaacg gatacgacga tttcctaagt ggtcgatatc atcgacattg
    30121 ccgatacctt ccatcaagtt caagaaatag ctcattgaag caatgatgtc ggctggacga
    30181 accgttttta cagctgcttc tggatagccg tttccgatca cgtttacttc acgttcaggg
    30241 tctcttggtg aaaatacttt gatgacttgg acagtcattg gatctgtgac cacaccgtct
    30301 tctgatggat agtaagtcac tgaatttagg ccattatcaa tgaaaggagc tagtgtttcc
    30361 atgacttggt gagtcagaac tgttcctttt tcaacgatga tctcgcctgt ttctggatca
    30421 actaatgttt cagctaatgt cagattcaat agacgtgttt tcaaatctaa ttttttgttt
    30481 actttgtaac gaccaacgtt tgctaagtcg taacgttttg gatcgaagaa acgagcattc
    30541 agcaagttac gtgagctgtc tgctgttttt ggttcgcctg gacgcaaacg ttcgtaaacg
    30601 tctttcaaac cttcttctgt tcttgagtcg cttgcgtttt tatgcaagtc tttttcgact
    30661 gtattgcgta atgtttcgct gtcgccgaag atttcaaaga tcgtatcatc ggaaccaaat
    30721 cctaacgcac gaactaatac ggttaaagga atcttacgtg tacggtcgat acgtacataa
    30781 gagatgtctt ttgcatcagt ttccatttct agccaagctc cacggttagg gataacagta
    30841 gaaccgaatc cttcttttcc gtttttgtcg acttttccat ggaaatagac acctggtgaa
    30901 cggactaact gagaaacgat tactcgctct gccccgttga tgatgaatgt tccttgttct
    30961 gtcattaatg ggaaatcacc gaagaatact tcttgtgctt tgatttcgcc agtctcgcgg
    31021 tttgtcaaac gcaatgttac atgtaatggt gcagagtagt ttgcatcatg cgcacgagct
    31081 tctgctactg tatactttgg ttctttcaat tcataatcta caaattccaa agatagattg
    31141 ccattgaagt catcgattgg caaaatgtct tcaaacattt ctctaagtcc ctcatctaag
    31201 aaccattggt aagagtctgt ttgaatttcg atcaaatttg gtaattccaa cacttcactg
    31261 attcttgcga aacttctacg ttcgcgatgt tttccgtatt ttactacgtg tccagccaag
    31321 ctcttcaccc ctctaaaatg tcattcaaaa agactacccg atcaattgtt acaataatat
    31381 ttcaaatatt atttttaggt aacaacagac attttttgcg gcttttaggc aaaaaaaaac
    31441 caaaaataga acattctctt gaaaccttac ttcgtgagct tctacttttg tttccttata
    31501 ttaaagccga aacggacaat atttcgtttc tgccttttct tcatcagata gtttttgcaa
    31561 cttcatatac tacacttatt ttctataaaa gtcaacactt cctggcgttt cttctgcttt
    31621 ttcgcaacat tttcttaatg ttttcaattt tatgttttca attatctgac aattcttaat
    31681 tgacagacct ttttatctcg gttatacttt agaaaattca ttttgtggag gaacttctat
    31741 gcgaaccaac atcaaccaaa tcgcaccaac acaactgaat aacttttact ttagctactt
    31801 tagataggtt tgtattcatc atgatggata caaacgttaa acgacagttt gtatctatga
    31861 tggctaaaga actttgtatt gcagcaaaaa gccacgtaga caaaatgcta agtggctgag
    31921 cagtgaaaat acaaagtaaa agtatcaagc attctacttt ttcaatttat tttccgactt
    31981 ttcaatccat ttggacattt tgtctgaatg gattttttgt ttcccaaaat taaggaggaa
    32041 aaagaaatga aaaaaaatat aatcatcgga ttaggcgctg ccctgctact agctttagga
    32101 ggttgcgcac aaaaagacaa ttccgcagag gcatcagatg gacaaaaaac aattggcgta
    32161 ttgcaacttg tacaacataa ctcgttagat gctgcctata aaggattcaa agaaggatta
    32221 gcagaagaag ggtataaaga aggcgataat ctaacaatcg attatcaaaa tgcccaaaac
    32281 aatcaagaca acctgaaaag tatgagtgag aaactcgtca aagaagatcc tgatttgcta
    32341 cttggaatcg ccactccagc agcacaatct ttattaaatg aaacaaccac tatcccgatg
    32401 actgtcacag cggtcacaga cttggaacaa gcccatttag tcacatccaa tgaaaaacct
    32461 ggcggcaatg tgactggcac gacagatatg gtgccaatcg aaaaacaaat cggcttactg
    32521 ttaaatatcg tgccagacgc caaaacaatc ggcatcatgt ataacaacgg tgaagcaaac
    32581 tctaagatcc aagctgattt ggcagaaaaa gaattgaaaa aagcgggcgt caaagtcaaa
    32641 gtactgacag caaattccac aaatgatgtc caacaagtga cgaaaagtct agctaaagat
    32701 gtagatggaa tctatattcc tactgataac acgttcgcaa gtgcggcaag tgttgtcggt
    32761 gaagtcgcaa aagaaacaaa aacacctgtc gttgctggcg cagttgatca agtcaaagac
    32821 ggcggtctag caacagtagg aatcgactac gaaagcttgg gcaaacaaac agggatcatg
    32881 gcagcaaaaa tcttagacgg caagaatcca agtgaacttc ctgtcgaaac agcgaatgat
    32941 ttggaactgt atgtcaataa agacatggcc aaagcattag gcatcaatcc agacagcatc
    33001 aaagaagctg aataaaacac aggaggattc tcatgacaaa tttgctctct cttttcttat
    33061 catctgcatc gcaaggggtc ttatgggccc tacttgcgat tggtgtatac gtcactttcc
    33121 gtatcttgga cattgccgac ttgactgctg aaggaagctt tcctttaggt gcagggatcg
    33181 cggcagtaag tattacaaat ggctatcatc cattgacagc ttgtcttcta ggctttttag
    33241 ggagtgcggc agcagggctt gtttccggac tgcttcacac aaaattgaaa attcccgccc
    33301 ttcttgcagg gatcatcacc atgactggtt tatattctgt cacatcccgt gtgatgggtg
    33361 cgccgaacat ctctttattg ggtcagccaa cgatctttca ttgggtcgag tcattcggca
    33421 taacaaaaga caacgctgta ctgatcgtcg gtctgctttt tgctttgatc gttgtatgtt
    33481 tacttgtttt gttcttacgt acagaaacag gacttgctat tcgtgcaact ggcgataatc
    33541 tggcaatgag cgaagcaaac gggatcaaca cagataatat gaagatcatc ggttatatga
    33601 tttctaatgg atgcattgct ctttctggct ccttgcttgc gcaaaacaac ggctttgctg
    33661 atttaaattc tggtattgga acgatcgtaa ttggacttgc ttctattatc atcgcagaag
    33721 ttttgttacg taaccagcca ctatttctcc gtctcttgac tgtcgtttta ggtgcggtca
    33781 tttatcgttt tattttagcg ttagtcttcg aactgaatgt tgaaccaagt gattcgaaac
    33841 ttgcttctgc tttcgtgcta gtgatctgtc tttctttccc gcagatccag caaaaattcc
    33901 atttacctca aaaaaagaaa ggcggtagtg tcaaatgaat gacgtcttaa cattggaaaa
    33961 tctccatcat acctttgaaa aaggaacgat caacgaaaat catgtcctga aaggcattaa
    34021 tctttcctta gatcccggcg acttcgtgac gatcatcggt ggaaatgggg ctggtaaatc
    34081 tactttgctg aacagcatcg ccggtacatt ttctgtcgat cagggtcaaa ttttattgaa
    34141 tggaaaaaat atcacgaaaa aatctgttgt cgaacgttcg aaatccatca gtcgcgtatt
    34201 ccaagatcct aaactaggga cagccgttcg tttaacggta gaagaaaatc ttgctcttgc
    34261 tatgaaacga ggaaaaaaac gcggcttctt ccgtggtgtc aaaccacaag accgaacatt
    34321 tttcaaagac cagctggttc ggctaaattt ggggttggaa aatcgtttat cctcagaaat
    34381 cggtttgctg tctggaggac aacgtcaagc tattacgtta ttgatggcca cattacagcg
    34441 tccagaactg atcttattag atgaacacac tgcagcgctt gaccctgcta cttccaaaac
    34501 ggtgatggaa ttgacagaaa agctgatcac tgagcagaat ctcactgctt ttatggtcac
    34561 tcatgacatg gaagacgcga tccgctatgg gagtcgactg atcatgctgc atcaaggaag
    34621 gatcgtcgta gatatccagc aagaggaaaa acagcagctc acagtgcccc aattattagc
    34681 gttgttcaaa caaaacagcg gcacggcatt aaaagacgat caagtacttt tatcttcata
    34741 atcagaaaga ggatgggaca aaaaatgaac gcccaccctc ttcttttttc tctttccttc
    34801 tttcgttttt gctttatgta tcaaccgttg tttcttcgaa aactattttg ttctctcctt
    34861 cttcctgata gaatggatgg gaaaggagct tgatgccatg acaacgaaag acaaggtact
    34921 tcaattattg aaaaagacac cagattttct ttccggagaa aagttggcac agcaactgga
    34981 agtttcccgg acaagtattt ggaaagccat caaagaattg gaaaaggcag gctatcgttt
    35041 tgagcacggg cctacagggt atcgttacct gccctctgac gtactagatg caaaagaaat
    35101 ccagcaagag ctacggtcgt ttattcctga tttgaaagta caaacgatga tgacttctga
    35161 atctacgatg aaagatgcca aactggcagc agctcaagga gattctgttt cgaagttatt
    35221 tgttgctgat acacaagaac aagctcgtgg acgttttgga cggccttttt tcgctgaacc
    35281 tggacgagga atctacatga gtctcttgct ccatccagac aaacggtttg atgaattgcc
    35341 tcaatatacg atcatcgcag ccagtgcctg tgtgaaagcc attgaatctg taaccggtcg
    35401 ttctgcttcg atcaaatggg tcaatgatat ctacatggat ggaaaaaaag tctgtgggat
    35461 tttatctgaa gcagtcagtg atatggaaag cggcaggatc agtcatatca tcattgggat
    35521 cgggcttaat ttttctgttc ctcaaaatca ttttccagct gatctccaag aaaaagccat
    35581 ctctgtcttt cccgacggac aagctagtgt cacaaggaat cagctaatca gcaaaatctg
    35641 gcaagaattg ttcacttatc tttctcaccc agaaaaagcg ttggacttct ataaagaaaa
    35701 atcattcgta ttgggaaaaa ccgtgtcgtt caatcaaaaa ggaacgactt atgtcggtct
    35761 tgcaaaagaa atcggtgatc atggcgaact gatcgtccaa acaaatgaac agaagatgag
    35821 tttgtcttct ggcgaaatca gtctcacatc tatccgtacg caataattat cagatttcac
    35881 aaaaggacac ctgatctcat acagtgtcct tttttctgaa ttctcttcaa aagactctcc
    35941 ttattctatc agctgacagt ttctctatcc tctagaaaaa ggtattctaa aaatatctat
    36001 aaagcatggt acactttctt taaatgaatc aatgcaaaaa acaagagagt catcagttta
    36061 cacgatcaaa agagatagca ggccgcttcc tttttcaatc ttatagatag accatgggta
    36121 acgataaaaa caaaaaagaa cttgcgactt attttgccgt aagttctttt ttagctagtg
    36181 ccttaatctc ttaatcccaa caaacgtctc gcatagatca cctgaccaga gtgcatagca
    36241 gcatcatcaa taatagaaac gagacgcatt cctcgtgtga ctggtggtgt ccagttctcg
    36301 tcaattactt catctagctc ggaaggttcc agccttctta agtaagaaat agcaaaatca
    36361 gcagctcctc gcaagtatcc tagcagatct tctatttgat tagcttggat ctgctttgct
    36421 tgttccagac tatgcttcca atcttgttcg ttatctgcta tcgtcatcgg aaaccggctt
    36481 tcccagtctt gactatgcca aattggagaa gttcctgcta aatcagcaat ctggaaatct
    36541 aattctcgag cggtatgcca agccagccaa gtcattgatt tgatctgcgg ggctctatct
    36601 tccactggaa aacgattggc ctcctcaata gagacgcctt caaacaaacg ttcaaaccga
    36661 tcgtatgccc gatccacact ctcgcatagc atatctaaat aatccattta ttttcctcct
    36721 ttttatttat cctacttact ttttcctaat tctaggatac ctacttatat gttactacac
    36781 acccttattt tttacacttt aacagtcacc atcatcaata gtttggagat tgaaagaaaa
    36841 agatagtgtc gaactatcaa ttgaacaaga agcaacaata tgaacgtttc attaatattc
    36901 tttaaataaa caatgatttt tcaaacgaac agtttaagac taggagaaaa agagtataat
    36961 aggaaaatgt ttgaaaggaa aggaagggga aaaatgaaaa aaagggcaag tttactagtc
    37021 ctagttgctt tgttgttcaa tatcttttcg tatggtgtgg ccgtttcagc ggaaacatac
    37081 attcctaaag accctgggaa tatttataat gatctaactg gtaactatgg cttgttagga
    37141 attgctagtc aattccacgt atttgctaaa aataagacaa ctgtgaatgc acatacagac
    37201 ggaaatatag caacacatga acttgatgca aaaaataatt ttggtacaga gcttcatgaa
    37261 ggtttattga accaagaaat aaattatata caaaaaactg attctttcgc taattcttca
    37321 ggcataccta caggtagcac aagaatgaat aaatttgtcg ttggcagtaa cgtggatgta
    37381 gcacttcaag atggtcatcc tacagtcaat ggatccaggc ttgatcactt aactccagat
    37441 gatttttata aagatcgtgc tgggcatgaa tatattgatt tcgagcagga atttgctaaa
    37501 ttgaatgtag cagcaaatga tcttgcaacg atcactccag ctaaaacata tactgctgca
    37561 gattttccag acatgaacaa tcggacaatc gatttaactg atttatcaga taccgggttt
    37621 ttattggtaa atatcgatgc agaagtgtta acgatgaata cgccacttca aatcattaat
    37681 ccaaatgatc aagtagttgt atttaatgtc atcaacagtt cttctgcatt gaacgtacag
    37741 tcaccaatca aatacaatga tcgttcaaat catgaaacag aagatttttc ggatgcgaat
    37801 atctcttgga attttggaaa tgagatgact gatttgacaa tcagtgctcc ttttcaagga
    37861 acaatccttg cgcctaatgc tactatccgc attactcaaa accaagacgg aacgattata
    37921 ggaacaaatg tgattttaga tgcagctacc aatcgttggg atcctaacga aatatttatc
    37981 agcgacaatg gtacagatac gacagacaca agcgacagta cagatacatc taccacaagt
    38041 gatagctcag attcatcaac ttcaagcgac agtacagata ctacctctac cacaagtgat
    38101 agcacggata catcagcttc aagcgacagt acagatacta cctctaccac aagtg
//
LOCUS       DAZKOR010000016        36568 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000016 DAZKOR010000000
VERSION     DAZKOR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.016"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8ZM_000771"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8ZM_000771"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0491754.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8ZM_000772"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8ZM_000772"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0491755.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8ZM_000773"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8ZM_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0491756.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8ZM_000774"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8ZM_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0491757.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8ZM_000775"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8ZM_000775"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0491758.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8ZM_000776"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8ZM_000776"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0491759.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG8ZM_000777"
     CDS             complement(8053..8523)
                     /locus_tag="ACG8ZM_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0491760.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG8ZM_000778"
     CDS             complement(8569..8994)
                     /locus_tag="ACG8ZM_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0491761.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG8ZM_000779"
     CDS             complement(9061..9330)
                     /locus_tag="ACG8ZM_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0491762.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG8ZM_000780"
     CDS             complement(9341..10936)
                     /locus_tag="ACG8ZM_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0491763.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8ZM_000781"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8ZM_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0491764.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8ZM_000782"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8ZM_000782"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0491765.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ACG8ZM_000783"
     CDS             15402..16376
                     /locus_tag="ACG8ZM_000783"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0491766.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ACG8ZM_000784"
     CDS             16546..17946
                     /locus_tag="ACG8ZM_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0491767.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ACG8ZM_000785"
     CDS             complement(18024..18746)
                     /locus_tag="ACG8ZM_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0491768.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ACG8ZM_000786"
     CDS             complement(18825..19331)
                     /locus_tag="ACG8ZM_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0491769.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ACG8ZM_000787"
     CDS             19454..21253
                     /locus_tag="ACG8ZM_000787"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0491770.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ACG8ZM_000788"
     CDS             complement(21321..22895)
                     /locus_tag="ACG8ZM_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFQ0491771.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8ZM_000789"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ACG8ZM_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0491772.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8ZM_000790"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ACG8ZM_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0491773.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ACG8ZM_000791"
     CDS             complement(24672..25310)
                     /locus_tag="ACG8ZM_000791"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0491774.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8ZM_000792"
     CDS             complement(25311..25961)
                     /gene="rpe"
                     /locus_tag="ACG8ZM_000792"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0491775.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8ZM_000793"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ACG8ZM_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0491776.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8ZM_000794"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ACG8ZM_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0491777.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ACG8ZM_000795"
     CDS             complement(29104..29844)
                     /locus_tag="ACG8ZM_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0491778.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8ZM_000796"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ACG8ZM_000796"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0491779.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8ZM_000797"
     CDS             complement(31215..32153)
                     /gene="fmt"
                     /locus_tag="ACG8ZM_000797"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0491780.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8ZM_000798"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ACG8ZM_000798"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0491781.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8ZM_000799"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ACG8ZM_000799"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0491782.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8ZM_000800"
     CDS             complement(35313..35618)
                     /gene="rpoZ"
                     /locus_tag="ACG8ZM_000800"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0491783.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8ZM_000801"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ACG8ZM_000801"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0491784.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7988 c         6705 g        11989 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaata
//
LOCUS       DAZKOR010000017        36472 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000017 DAZKOR010000000
VERSION     DAZKOR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.017"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..598
                     /locus_tag="ACG8ZM_000802"
     CDS             278..598
                     /locus_tag="ACG8ZM_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0491785.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            752..1657
                     /locus_tag="ACG8ZM_000803"
     CDS             752..1657
                     /locus_tag="ACG8ZM_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491786.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1654..2427
                     /locus_tag="ACG8ZM_000804"
     CDS             1654..2427
                     /locus_tag="ACG8ZM_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0491787.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2608..4032)
                     /locus_tag="ACG8ZM_000805"
     CDS             complement(2608..4032)
                     /locus_tag="ACG8ZM_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0491788.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4243..4689
                     /gene="nrdI"
                     /locus_tag="ACG8ZM_000806"
     CDS             4243..4689
                     /gene="nrdI"
                     /locus_tag="ACG8ZM_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0491789.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4754..6736)
                     /locus_tag="ACG8ZM_000807"
     CDS             complement(4754..6736)
                     /locus_tag="ACG8ZM_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0491790.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6750..7646)
                     /gene="murQ"
                     /locus_tag="ACG8ZM_000808"
     CDS             complement(6750..7646)
                     /gene="murQ"
                     /locus_tag="ACG8ZM_000808"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0491791.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7649..8710)
                     /locus_tag="ACG8ZM_000809"
     CDS             complement(7649..8710)
                     /locus_tag="ACG8ZM_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0491792.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8921..9448)
                     /locus_tag="ACG8ZM_000810"
     CDS             complement(8921..9448)
                     /locus_tag="ACG8ZM_000810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0491793.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9515..11017)
                     /gene="eat(A)"
                     /locus_tag="ACG8ZM_000811"
     CDS             complement(9515..11017)
                     /gene="eat(A)"
                     /locus_tag="ACG8ZM_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0491794.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11275..12396)
                     /locus_tag="ACG8ZM_000812"
     CDS             complement(11275..12396)
                     /locus_tag="ACG8ZM_000812"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0491795.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12522..13463
                     /locus_tag="ACG8ZM_000813"
     CDS             12522..13463
                     /locus_tag="ACG8ZM_000813"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0491796.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13613..14509)
                     /locus_tag="ACG8ZM_000814"
     CDS             complement(13613..14509)
                     /locus_tag="ACG8ZM_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0491797.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14778..16118
                     /locus_tag="ACG8ZM_000815"
     CDS             14778..16118
                     /locus_tag="ACG8ZM_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0491798.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16336..16779
                     /locus_tag="ACG8ZM_000816"
     CDS             16336..16779
                     /locus_tag="ACG8ZM_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0491799.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17283..18701)
                     /gene="nhaC"
                     /locus_tag="ACG8ZM_000817"
     CDS             complement(17283..18701)
                     /gene="nhaC"
                     /locus_tag="ACG8ZM_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0491800.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19104..20327)
                     /locus_tag="ACG8ZM_000818"
     CDS             complement(19104..20327)
                     /locus_tag="ACG8ZM_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0491801.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20358..21197)
                     /locus_tag="ACG8ZM_000819"
     CDS             complement(20358..21197)
                     /locus_tag="ACG8ZM_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0491802.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21214..21894)
                     /locus_tag="ACG8ZM_000820"
     CDS             complement(21214..21894)
                     /locus_tag="ACG8ZM_000820"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0491803.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21896..22969)
                     /locus_tag="ACG8ZM_000821"
     CDS             complement(21896..22969)
                     /locus_tag="ACG8ZM_000821"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491804.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23025..23263)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23306..23539)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23616..24287)
                     /locus_tag="ACG8ZM_000822"
     CDS             complement(23616..24287)
                     /locus_tag="ACG8ZM_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0491805.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24374..27022)
                     /gene="mgtA"
                     /locus_tag="ACG8ZM_000823"
     CDS             complement(24374..27022)
                     /gene="mgtA"
                     /locus_tag="ACG8ZM_000823"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0491806.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27364..27538)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27711..28148
                     /gene="ndk"
                     /locus_tag="ACG8ZM_000824"
     CDS             27711..28148
                     /gene="ndk"
                     /locus_tag="ACG8ZM_000824"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0491807.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28170..29036)
                     /locus_tag="ACG8ZM_000825"
     CDS             complement(28170..29036)
                     /locus_tag="ACG8ZM_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0491808.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29196..31289)
                     /locus_tag="ACG8ZM_000826"
     CDS             complement(29196..31289)
                     /locus_tag="ACG8ZM_000826"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0491809.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32038..32661)
                     /locus_tag="ACG8ZM_000827"
     CDS             complement(32038..32661)
                     /locus_tag="ACG8ZM_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491810.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32820..34217)
                     /locus_tag="ACG8ZM_000828"
     CDS             complement(32820..34217)
                     /locus_tag="ACG8ZM_000828"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0491811.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34249..35220)
                     /locus_tag="ACG8ZM_000829"
     CDS             complement(34249..35220)
                     /locus_tag="ACG8ZM_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0491812.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35594..36019
                     /locus_tag="ACG8ZM_000830"
     CDS             35594..36019
                     /locus_tag="ACG8ZM_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491813.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        10552 a         7603 c         6391 g        11926 t
ORIGIN      
        1 ttactgtttt ttttaaatga ttaataaata acaacttctt tctatgaaat tgcaaagcat
       61 tcatcttctt ttccgctaac gatatatccg tcagtgcttg gtacacttta ctaaaaagta
      121 tgagattgcc gatatctgga taggtgtgct atactacaga aaaagatata caatctgcca
      181 ccgggtagga taaagaggat gatcattcca ttaggtcttt gaagggatga ttttcctctt
      241 tttgtgtatt tttttatagt taaggaggaa tgtaagtatg gcatggatgg aattaatcat
      301 agcaggaatt ttggaagtat tctggtcaac gatgatgaaa tggagtgatg gtttttctaa
      361 aatcaattat tctttttaca cagtgattgg catgattgct agtttttact ttttgtcaaa
      421 ggcaatcaaa agtttgccga tgagtctctc ttatcctatc tggacaggga ttggcgcagt
      481 tggttcggtc ataattggtg tggtcttgtt tcatgataag ctgaatcttt ccacttggtt
      541 ctttgtgggg atgctactca tcagtatcat tggcatcaag attactagcg gccactagac
      601 acttggaata agtactgatc agaaaaaata agccttttta tttaatattc aattttttta
      661 tgaaaaaata agaaataagt ccaatgacct agcgacttct ccactcatca ggtgtatgct
      721 tgtactatct atagaaaagg agtgaatatt tttgaaaagt ctttcggtcc aagaattatc
      781 caagaacttt ggtcacaaac aagccctcag cgatgtcagt tttagcttat cccctggcaa
      841 aatcgttgga ttagtaggtc ctaatggcgc tggaaaaaca acgatcatga aagctatttt
      901 aggactgatc cattactcag atggaacaat ctgtattggt gaaaatgaga tttctccctc
      961 tagtcataaa gggttggaac aagtaggagc attgatcgaa tacccgggta tctatccatt
     1021 cttaacaggc tatgatcatc tgaagttatt ctctgaaacc aacgatgtat ctgctatcga
     1081 cactatcgtg aatcagctca agatggaaaa gtacattcac aaaaaagcga aatcctactc
     1141 gttgggaatg aaacaaaaat tgggaattgc tcttgcctta ctgaaccatc cgtctatcgt
     1201 tattttagat gagccgatga acggtttgga tccacaagca acaagagatg tgcgaaaaat
     1261 cattaccgac ttggctactc agggcacctc tttcttgatc tccagtcact tactcagcga
     1321 gcttgaaaaa atggtcgatg agctgatcct gatcaatcaa ggaaagatca tcaaacaatg
     1381 tacaatggct gaattgaacc aaacagcgca agatttcatc gttatctcta caacaaatga
     1441 tctagctgct cgaacgatct tgacagaagc tggatatact ttggaagatc ctgataaaat
     1501 caagctttta aaagaaaacg aagaattatt aggaaccgtg atccagctat tgtctgcaca
     1561 aaatatccat gtgacagacg tccagcatct gacgaatgat ttggaatctt ctctattgga
     1621 aattctggat tcagtaaagg gggaagaaga aaaatgacat tgcttgtaaa acaagaatta
     1681 tttaaattga tcaagaaaaa gtcaactgct gttttatctg ttctattagt tgttttgctc
     1741 ataggaacag cacttctagc caaaaaatac acgacgatca ttgatccagt tgaaatgact
     1801 gctcagcttt ttagtgccac atcttggatt gtctttatca tgattgcagc tgcaagcacg
     1861 atcatttcga tggaagcaca atatggtaca ttgaaaaatc tcctttatcg aaagtattct
     1921 cgaggagaaa tattagtaag caaatggatc acattagtta tttattctgt ttatttatac
     1981 cttttagcta ttattgtcac tgtgttgatg aaactgattc ttttcccttc gatttccttc
     2041 acggagaagg tttctactgg tcaaacactg atccaaagct tgttcactta tactttgggt
     2101 agttatatcg gtttatggct gattttaagt ttagtgttga tgattgcttg ctttatcaat
     2161 agttccggcg catccatctc agcaggaatc gtcttttatt ttgcttcgtc aatcatatct
     2221 ggtatcctta ttgcactcat ccaaaaatgg gagtggatca aatggaatcc gatcagtatg
     2281 ttgaatctgc aaaaccaaat tggaaacgaa gagatcatga agcaattgac tcatctttct
     2341 actaatcaaa tgctattcgg taatttagct tacatcgttt tgttcttagc attaggatat
     2401 ctcgtattta agcgaaaaaa tgtatgatga ctcatcaaaa aagaggctgt gaaagagacg
     2461 ttcagcctta ttgccgattg gctgtctctt aaacaccgtt tattctgaaa aaggagctgt
     2521 gacaacattt gtgtcacagc tcctttccta gtatgttttt gaataaacga caggctcaca
     2581 acctctcttt atacttcttt tatacttcta tacgtgaagc atcccagtgg caaataaaac
     2641 cattgcaatc actccgacca tcgtcacacc tataagtaaa atcttatcta agtggatatg
     2701 ttgtacaggc tcgttttgct ccttacgtgc tttgaggtaa aaataaatgc ctggcaagaa
     2761 aagaacaaga acaagcatta cttcattaaa ggctacgact agcatagccc atacttgaaa
     2821 ggcacttgct aaaagcccta ctaaaaattg gacccattct ttccgctgat aagaaagttt
     2881 catttggtac aatcctaaga aaagccacgt gaagaaaata gctgctgtac caagtgtgta
     2941 agcaaagtta taagcactgt cagtgaataa taatgaaagt aaaaagacat tcgtacataa
     3001 cgtcgtcaat acgatagcat aagttggtgc cccatttttg ttgagttttc cccatgcttt
     3061 aggcaaaagt ccgtcttcag ccatgtttcc cattgtttca atcggtaaga tcgtccatga
     3121 gagccaaaca ccaactaaag aaatgatcaa accgccgttg atcacgactg ctccccattt
     3181 accaaccatc atttcaaaca catagcccat agcaggctgc gccttgatcg tttgcagctg
     3241 attcatcgtc attgtcccat atggaagcat ggaaaccaag acgtaaataa taatcaagcc
     3301 gaatatccct agaatcgttg ctttcccaac atctgattgt ttttttgctc gtgaacctac
     3361 gacagaggct ccttcaatac caacaaatac ccaaagcatc accatgatgc aactcaatac
     3421 ttgactggaa aacgggatcg gctgtccatt cgcattcaat ataatgtcgc tagaaaagtt
     3481 atcaatgaat tgttggatcg aaaacgtgct gatagctact acaataaata aaaataatgg
     3541 cactaattta cagaatgtga cgatagcatt gacgaaacta gcattttcga ctccccgatt
     3601 cactagccat gcatatcccc ataaaaagac tgtactcatc acgatcgatg ccagattttg
     3661 cccaccttca aatactggga agaaatatcc taatgcgctc atcaacaatg ttgcaaaagc
     3721 tacgttgcct aaccagcatg tgagccagta accccaaccg ctgataaaac cagcgaaacg
     3781 gccaaaacct tcttctgcgt aactaaagat cccgcctttt aaatctggac gttttttatt
     3841 gatattattg atcgacaagg agagcatcaa cattgctgtt cctacgagta cccaactgaa
     3901 aatagctggg ccgccagccg atccggagat gtccgtcata ataccaaata cgccacctcc
     3961 aatcgctgaa ccaattacta aggcagcaag tggaattaaa ccaattcctt ttttctgttg
     4021 ctctgtatcc atgtaagcat cctccaaaat aaaaccgctt ttatcaaaaa gtataacaaa
     4081 ccttttttat ttttacttaa ttaataaaaa ctttaataga aatataattc taccggcttt
     4141 tttctcatta tacaagcaaa gcgcctagtc cctttccaaa ttacaccacc aaaaaactta
     4201 aaaaagatgc tatactaaaa ggaaagaatt ggaggatgat ccatgaacat tttatatata
     4261 tcgatttcag gaaatacgcg tgcctttgcc aaacatctag cagaatatgc tgaaaaaatg
     4321 catgcagaag atcctgtcaa tcctgaagtt actttaaaag aaatccatga aaatagtgat
     4381 tttacgaaag aaacagaacc attttttact ttcgttccta cttatttaga cggcggaaat
     4441 ggtttagata acggtgatac agaaatcttg accgaaacga tgcgtgaata tctggaacat
     4501 gaagacaatc ataaactttg tcttggtgta gtcggtagcg gaaataaaaa cttcaataat
     4561 cagtattgtc taacagccaa acaatatgca caaacatttg gctttccttt cttggcagat
     4621 tatgaattac ggggaacacc atctgatgtg acacgaattt atcagatttt agcagaaaac
     4681 aataaataat aaaaaagaag aagctgaaaa atagatttca gtttcttctt tcgtttctat
     4741 tgtttctatc atattaagct ttgacatcca tcacaggtgc ttttgctttt gctttacctg
     4801 ttttttccaa gacaaagttt tttacacgat ccatattggt taccaagacg atcatggatg
     4861 tttctttgcc tgctgctttg atcgcttcta agtcagcttc tgctacaagc gtatcaggtg
     4921 tcacttgctg cccttctgtt actttcacat caaatggcgt accgcctaag tcaactgtgt
     4981 tgattcccat atgaagcaag atttctaaac catttggcgt tgtgatccca atagcgtgtt
     5041 tcgttgggaa gacagagctg actaccccag taactggaga atagatatgt ccgttttctg
     5101 gttctacaaa gtagccttca cccatcatct tttgtgcaaa aactggatca cttgctttat
     5161 ctaaagaaac aactgttccg tcagcaactg atgagaggat agtcacactt tcagctgaag
     5221 tttgaggtac tactttttca ggcacttcat ttactgtggc tgtttcagtc gctaattctt
     5281 ctttttctaa acggtcttta ggaataccga agaagaatgt agcaacgaag ccaccagcat
     5341 aagctgccaa taatccaagg atgtagccta accatttacc gtcagagatc aatgggatca
     5401 aagcggctcc tgaaggtcca atagcgatag ccccgacatt tccgatcatt ccgatcactg
     5461 ccccgccgat acctccaccg atacaagcag taataaatgg gcgtcctaga ggtaatgtta
     5521 ctccgtaaat caatggttcg ccgattccta ggatcccgac tggtaaagct cctttgatca
     5581 tttctgttaa ttctttatct tttttgcagc gaatccataa tgccaatgct gcacccactt
     5641 gtcctgcacc agccatcgca agaattggaa gcaatagcgt acgaccagtt tcatcaatca
     5701 tttgcacatg gattggagtc aaaatttgat gcagtccgaa cataaccatc ggcaagaata
     5761 atgtgcctag aacgaagccg gcaaatgctc caccaacgcc aagtacccag ttgatcccac
     5821 cgaccatgcc atcagaaatg aaacctgcta aaggcatgat gaagaaaatt tcaatcagac
     5881 cgatggcaat caatgaaaga gttggtgtaa cgatgatatc taccgcatca ggtactactt
     5941 tatgcagttt tttctcaacg attgacaata gccaaacagc gaagatgaca cctaggatcc
     6001 caccttgtcc agctgcaaga gcttcaccag taaataagtt tttcagtggt gcttccggat
     6061 tcatccctgt cagcatcaca acggccccga taactccacc aagagttggt gttgcaccaa
     6121 aaacttgtgc agcattgata cctgtataga taactaggta gctaaacatc ccgtttttca
     6181 agatgttcat cacatcgata tattgttgcc atgttgctgc atcaagtgtt ccggctgtga
     6241 ctaagtttgc taaaactgcg gcaatccctg ccacgatccc agtaccgaca aatgcaggaa
     6301 tcattggcac aaagatatta gaaatgtctt ttaataccga tttgatttta gatggttttt
     6361 gtttcgattt ttgtgccgct ttcatttctt gtgcccgttc gtttacacgg tcttttccag
     6421 atgctgatga atttgctgta gctgtactta cagaagaaag tttttctcct aatttcacgc
     6481 ctgccaaatc aaccatttgc tgtgctactt tgttcacttt cccaggtccg atgatgactt
     6541 gaagctgttc atcttctact acaccaagca ctcctggaat cgcttttaat tcagagacat
     6601 ttacaagatc atcattgatg atcttcatac gcacacgagt catgcagtgg acaatgcttt
     6661 ctacattgcc catgccgcct gcccctttat aaatttgttt tgccagttct tccaaagtca
     6721 gttcgttttt tgccatggtc ttttccccct cataaggtcc cttttacaaa tccgtttgct
     6781 tcagctaatt ttgcttgagc ttcctcttta tcactatttg tcaaaatcat gacgatagcc
     6841 agcttaacat tttgatctgc ttcttcaaag tacttttcag ctgtttcgta atcagcttct
     6901 gtcgcttgca tgatgattcg ttttgagcgt tcgattagtt tttcatttgt tggtttgaca
     6961 tcgaccatca agttattgaa tactttacct ataccaatca tactgatagt agaaatcata
     7021 ttcaagataa gtttttgtgc tgttcctgac ttcaatcgag tagagcctgt caaaaattct
     7081 ggacctgcat cgacttcgat tgggaaatcc gcgtgtttac tgatttcggc ttgtttattg
     7141 caagaaatcg ttcctgtgac agcaccgatc gattttgcgt aatctaatcc accgatcaca
     7201 tatggtgtac gaccgctagc tgcgatacca ataactatat cattttttgt caaattcagc
     7261 ttgattagat cttcttgccc tagctcataa gaatcttcgg ccccttcgac agctacagtc
     7321 attgcttctt ctccaccagc aattagacct atgaccattt ccggttctac accgaatgtc
     7381 ggtacgcatt cagcagcatc taacacacct agtcttccac ttgttccggc tcccatataa
     7441 atcagacgtc cacctttttt aaaggactca atcgtgttag caatcactgg ttcgatactt
     7501 gtcagttctt tttcaaccgc agcggccact ttctgatctt ctttgttcat tagttgaacg
     7561 gcttgagaaa tactcatctc atctaatcca aaagtttctt ggtttctgcg ttccgttgtt
     7621 aggttctcta aattgatttt gctcattttt atcttccttc ttttcttata aattgatagt
     7681 tcataccagc tttgatttgg ttgatcaatg gtagctcttc tttgataact ttagctacta
     7741 cattcacttt ttcatccgct ggcaaatcat atttggttaa ttggatctca cccatatagc
     7801 gcaaatactt ttcgttatct aaagtaatag accctttcat acgcgtagct gtatttcttg
     7861 ctgggatagg cggaatctct cgaaaacgtg catctacgct tcgtatgaca tctcttgcat
     7921 cgtcttgccg attcgtatgc ttccctaata tatattcgta aaattcttca tctacaggtt
     7981 ccgtatggag caagactgtc tgttcttttt ggaaagaagc aaattgctcc tgaacgtttt
     8041 ctgacagacc tgcatctccg atgtaaacaa tatccgtgtt acttgctaat agatctagag
     8101 cgcctgcaag aggatggatc cctcgatgtt cttccaaagt tggcaatcct tgatatagtg
     8161 ggcctctcaa ctctgtatct cctggtacaa aaccttggac aaggagacct tgaattttga
     8221 gccattgatt ttttttctga taccattctt tgtccagccc tgtttcggga cgtggataat
     8281 aattatgcca agcttcaatc tgtgagaaat cagcttctgc ttctttcaat tcatcgatat
     8341 ctttaggcgt gatcgtgcta gcattcaaac tgattttcaa ttgatgtgac cagtcggcaa
     8401 tctgctgatt ggaaatatga taatccattc ttaaacctgt tacaccaatt gctttcaaag
     8461 gcgttagatc ttctaatgaa aaacccgctc gttccaatgc ctctcctgaa atatcgacca
     8521 tcaattctag tttattcttt ttcgcttcgc ttcctagttc gatcaatcgc ttttgatatg
     8581 ccgaaacatc atcttctggt atatgcatgg aagtgaaaat accgacaaag ccattggatg
     8641 ccatttggcg gatatactgt ttcttctctt ctgacaaaga atcgttcata aatattgaaa
     8701 agccaaacat attatccctc tttttctgta tccgtcttca tttctatgac tagagtatat
     8761 atctaaagaa acaaaatttc aatactttta ttaaaataat taaatttcat ttctaaaggt
     8821 tgagtttata ttatcaaaag aattgtcata acctaaaaaa ggcactgcta agcaaaagca
     8881 gtgccttgaa atattttatt taaataacac aaaaacataa ttactcataa gaaaccaatt
     8941 ctgtattatc taccatgaat gtaaagaacc cctcattagg catcggatga aaataagcat
     9001 tgatcactct tatcaaggca ttatggcaaa ctaacagata gacattttct tcgtcttcga
     9061 tgcattcttt caataaagga acgatccgtg catacacatc taagactgac tccccatttg
     9121 ggaatcgtac agcgaacgct aaacgtgctt tttgaaagtt ctcatctttg cttggcagtc
     9181 catcataatc cccaaaatcc atttctacca atcgttcatc aacggtcagc ggcaagctga
     9241 gacgatcagc aactgcttgt gctgtatctc ttgctcgttg aagaggggaa tggatgattt
     9301 tcgtgattgg aacaggcaaa tctactacct tttctgctag tttctgtgcc tgcatctgtc
     9361 ctttttcagt cagcggcaca tctgcccgtc cgcaaactaa tccttgtgcg ttccaagttg
     9421 tttctccgtg tctcgttaca taaagcatgt gattctctcc ttcgttggca aagttcccct
     9481 taaccatacc ttgttgttct tgagattaca agggttagat tctctcaaga gaaataatct
     9541 cagtagatac tttatccagg aaggtttgat catgttccac tagtaacatg gcaggtttca
     9601 cgttcaagat cagttgttct aattgttctt gattgaagac atccaaataa ttcaatggtt
     9661 catcccatgt atatagttca gctggctgtg ataaagattt agccaattcc acttttttcc
     9721 gttggcccat actcatctgc tcgatcttgt tatgaaaaac atctctttcc atcccaagct
     9781 ttcggaggtt gttcaaaaat gcttggtagt ctacttggtt tttctctgca aattccgcca
     9841 acgttcctcg attgtcttca taattttggc ttgcataact aatgctcaga tgttttggct
     9901 ggtatttttc tcctataacc ttgccgttga atgcccccag aagataatgg ataatggatg
     9961 actttcctgc accgttagga cctgaaatcg ccacccgctg atgaggctcg attgtaaaat
    10021 gaattggctc gaataacaga ttttcatacc ctaattgaag atcttctacg cttaaaagtc
    10081 gcttatggtg agacgcttgg ctattcatcg tcaaagaatc gatatattcg atatctttta
    10141 atagtttttc tttctcgctg atctgggtct ccatccgatt cacgattgct ttggatcgtt
    10201 tcatcgtccg tgcagcatca gcacccactg ctcctttatt cactcgtcta gattcagtat
    10261 cgatgaatcc gacttgtttc tttgttttat ctccttctcg ggaacgagac cattcggctt
    10321 tttcagctgc tgttttcttt agcctactga cttctttttt caatttttca ttttgagcca
    10381 tttcaaattc atcacgaagt tttttctgtt cttcatagat agagaaattc ccttgataaa
    10441 gttccagttg acttttttcg attgctaaaa catggtccac tacttcatcg ataaatcccc
    10501 ggtcatggct gaccacgatg aagccttgtt ttttcttttt caaataagcc gctacttgtt
    10561 ttctaccaga gatatccaaa tgattcgttg gttcatcgat taacgggaaa tgagtgtcat
    10621 ccacaaataa aagtgccagt aggacttttg tcttctctcc ccccgatagt gtgctgaatt
    10681 ctctccataa gatttcagga tctgtttgca tcaattggag ctctctttcg atttcccata
    10741 tctcaaaatc cgtaatatca tttaacacgt aataggttaa acgttcttta tcttttgtct
    10801 gttgcgggaa ataggcaaat tcttgctgat ggattacttg cccttgataa ggtagtttgt
    10861 tttgtagaat attcagtaaa gttgtctttc ctcgaccgtt tcgtccgata agtcctagtt
    10921 tccattgtgt gtcaaaattt agatttgctt gttcaaataa taatgtgcct tggctgtcgt
    10981 agccgaatgt cagatttttt atttcgattt tagacatttt ccatcacctc gtaaaaaaag
    11041 accagtccta ttttcagaac tggctaaaga ggatgcaagg gttggataca cacaaaaaga
    11101 cccatacatc gaaaaaacca agccaatccc gaaaatctgc acactgcttc cttgtctcca
    11161 caaggtacag tctgaatgca gctttcatca atcgacttaa tttttcaatg cacggatccc
    11221 tcactttgtc ctatatttga aattacgata gcataaagaa aatgattttg tcaactactt
    11281 cgggaggatg gtgaaaaatc tgctaagtgt ctgtcctaag atttttgttt tttctaatgt
    11341 ttgcctgtgt tccattgctt cttctaaacc tattggaccc aaatgaatac ttaagacaat
    11401 tgggagaaaa tcagaaagtc cttctagctc ttggtctcta cttccgcaaa tagcgattat
    11461 tggttttcga tatttttttg caagcactgc tacaccgtat ggaacttttc cgtggatcgt
    11521 ttgctgatcg atccgccctt ctcctgtaat gatgacatct gcttgttcga tctttttttc
    11581 caatccagtg atttggctga ctaaatcgaa accagaaacc atcttcgctc ccaacaaaaa
    11641 aagggctcca ccaataccgc ctgctgctcc acttcctgga atatcatcta aatccacctc
    11701 ccagaaatct tttatttgct gagcaatctt tcctgcttgt ttatctaaga aagctagcgt
    11761 cgtatctatt cctccttttt gtcttccaaa ttgccaagca gctccttgtt gacctgtata
    11821 tggattggta acatctgcag cgataatcaa ttttacttta gaaacaagaa cgtttcttcg
    11881 atcaaaatga agttttttag tagataataa aggatttcct tctgtgtacc cttctaaaga
    11941 agcacccaag gattggagta atcctagacc accatcgcta cacccgcttc ctcctaaagt
    12001 gacaataatt tgttctactt cttgaagcag tgcatcttta ataagttctc ctaaggcata
    12061 actggaagca agtcggatag tttgatccgt tggtttatca agtagatcta accccaatgc
    12121 ttgtgcagat tcgatgactg cggtgttttt tccttgccaa gttgtcaata aatagctgat
    12181 ttttcgctga cggaataaga gatccatccc atcaatggta cgccatgttc ccctgagcgc
    12241 gtaatagatt gcttctgcag tattttctcc gccatctgca atcggcacat tgatttttct
    12301 tcctttacga tcttcccagc catttagaac tgcctgattt agttcaaatg aagaagcact
    12361 tcctttaaat gaatcaattg ccgccaaaat atccatctgt atccctcact ttcttcttaa
    12421 gtaagacact ttttttcatt tttttcaaac aaaacaatgc tattttcttc catttttcta
    12481 tatactaaaa gggaactcat tgaataaagg agagaaaaaa catgaaaatc gcaatagctg
    12541 gggccggagc aatgggcagc cgaatcggat tgatgctaca tcaaagcgga aatgaggtcc
    12601 tcttgatcga tcgttggcct gctcacattg aagccattcg tacaaatggg ttgattgctg
    12661 actttaacgg taaagaagtg gtcgcaaaac tgccaatcta ttcaccagaa gaaatcatcg
    12721 aaagcaacga acacgtggac ttgatcgtag cattaacaaa agccaatcaa ttagatgata
    12781 tgttttgttc gatccaatcc attattactg acaatactta tgttttgtgt ttactaaatg
    12841 gattaggtca tgaagatgta ctggaaaaat atgtgccaaa gaaaaatatc ttatttggga
    12901 tcacgatgtg gacagctggt ctagctggtc ccggaaaagt cacattgtta ggtgatggag
    12961 aaatcgaact agagaacttg gaaccagaag gcgaagcatt tacaaaaaaa gttgtagaag
    13021 tctttcaaga agcaaattta aatccgatct atagccataa cgttcgttat tccatttggc
    13081 gaaaagcttg tgtaaatgga acattgaatg gtttatgcac gattttggat tgtaatattg
    13141 ctgaactagg cgcgcaaaaa gcggcagaga gcatggttcg aacaatcgta tctgaatttg
    13201 cttctatcgc agcaaaagaa ggaatcatac tagatcaaga agaagtgtac caacatattg
    13261 cttctactta tgatcctgac aacatcgggt tgcattaccc atctatgtac caagacctga
    13321 tcaaaaacca tcgtttgact gaaatcgact acatcaatgg tgccatttgg cgaaaaggac
    13381 aaaaatatga tattgctaca ccatattgtg cctttttgac ccaattagtc catgcaaaag
    13441 aaggaatcct tggcgcagaa taaaaggctc tctgatgaga atcagctgaa tcagcagctg
    13501 gttctctttt tttaagccca ataaaagatc aaaaagaaaa cacaactctc atgatgccat
    13561 aagaattgtg cttgtttgtt tcagtacata atctcttcaa aatgtaggtt ttttatttgt
    13621 tttcaacaga ggagaggtca tattcgtatt gctcataaat agtggaatcg ttagtgataa
    13681 atcgttttgc ccaattttct gcatcataaa attccaatag ctctgatagg attggcttaa
    13741 atggtgtatg cagaaattcc ggtaattcat ctaaatcacg ttctcgaagc aaaaaaatat
    13801 tttcaggacg ataaaaatga taatgagcaa tcgcttgatt atccgtcact agtttctttt
    13861 gaaaatacat ggattcaaat gtccgcaaag tcactccttg ctgaccctcc cgcaacagtt
    13921 ccagaatccc gcgcgattcc cctgtttttt tcaggtactc ttcataagta agatatccag
    13981 gttgttcatt ccctcgatgt ggaagaatga aatagttggt cgtaacatct ctttttataa
    14041 attcctgctt atagctttct gctcgttctt ttctcccatt attcgttgca ccaaaaaata
    14101 gatccgtctt tggttcgcta gaagaaaaat tcatatgttt cgaataaaat gaactattat
    14161 gtttcagccc aagttctttt gcttccaaaa aatcaaaatg ataaatctca tcgattgccg
    14221 gttcatcttg tgcctgcttc agcatcgcct cgtgttctgc tttgaagtga ttccaaaaaa
    14281 ataacacaat ttttgcattg gtaaatgggc gcacgcgtcg agccatctcc atatcaaatc
    14341 cataatctaa aaaaacgatc agctgataat ttgccaactg ttgtctagct gatttaatga
    14401 attccgactg tagtaaatct tttccggacc aaacatctaa agagcggatg gaaggcaaaa
    14461 aataaagtga tttatttttt gcaactagca gtacgtcatt tttgttcatc ttttccgcct
    14521 cactttctct gtcacttttt cctatgatag cctgtttaaa tagtaaaaag ccagtaaaac
    14581 gtttcctata tttgtatgaa aatttaacat ttgtatgaaa atatgatgaa ataaatcatt
    14641 cttctattaa aattcgccta aaagttttct gaatagttgt aattgtctga aaatttgtta
    14701 tcattagtca ttgttaaata ttgctattta ttcttaatat aaattttgat tgtgaattta
    14761 aaagaaagag gtcatttatg gaagataaaa aattgaaaaa acaacttagc tccagacata
    14821 tcaccatgtt agctttaggt ggagcaatcg gtgcaggtct atttaaagga agtggcgaag
    14881 cgattgggat cgccggccct tctgtattga ttgctttttt gattggtggg ctgattttat
    14941 atatcgtgat gaaaggtctg ggaaaaatcg ttttaagcgg tggcgacacg catcacggat
    15001 tatctggttt gatacgccct tatctaggtg ctcattctgc tgattttact gattgggtct
    15061 actggtctat gtggattatc aatatcatcg ctgaagctgt tgcagctgct tcctttttgc
    15121 agttgtggtt cccgcacatt ccagcctggg tatttgtttt tatgttggct gttttaacta
    15181 cgatcatcaa tctttattcg gtacggttat tcgcagaaac agaatattgg ctggcttttg
    15241 ctaaaatcag tgtcattatc ttactgatca tttttgctac ttatcttgtc ggtcagcaaa
    15301 tgttgggcac aggcgttttc cctacccttc agcaattgac tgaccatggt gggtttactc
    15361 ctcatggtat gaagggaatc atcagttctt tgcttgtagt tatctattct tacggcggtt
    15421 ctgaactgat tgctatcacg gtaagcgaag cagatgatcc taaacgtgcg attcctaaag
    15481 caatcaaagg tgtcatggga agaatcattt ccttttacat cattcctttg tttttgcttt
    15541 tgattctttt tccttggaat actttagcag gcacgaatgt cagtcctttt gtgatggtat
    15601 ttgaaaaaat gaatatccca tttgcagcgg atatcgtcaa ttttgtgatt gtccttgctt
    15661 tgttctcatc tatcaattcg ggtgtgtatg catcttcaag aattttgttc ttccgtttga
    15721 aggatcgaaa aggttcctct agaaaactag ctgttttaaa caagcatcaa gtaccgcaac
    15781 gtgctgtttt attttgtacc agtgttcttt atcttggtgt agctctttct tattttgtcg
    15841 gtgacaaatt atttggctat ctggcaggtt ccctttcgta tacagtatta ttgatctgga
    15901 tcattatcag tgcggcatcc tttgtactgg cactaagaaa aggaacatta tgggataaag
    15961 gaatcagctt ttttgcatta gctgtcttag gattgatttt ccttggtatc ctgttcacga
    16021 actccattgg ggtaactgta ttgactggcc tgctttacct gttcatttat ttcagttatc
    16081 aaaaaaagaa tgatgcgttc gtattgacga atgaatgaaa tcgatagaaa attttatttt
    16141 atttatgtgg aatagatgga gctattgaag aaaggagttg tgacaaaagt cttgttgcaa
    16201 ctcctttttc cgaataaaca gtctcatgac ctcttggtaa acggtgttca aaaaccctct
    16261 gtgcaaaata attgattgtt ctccatgctc atgacatgat agaagaaagg aatgaaaaaa
    16321 aggagtttgt tttaaatgaa aaagtattgg aagtttttgt tgatgatcgg tgtttcaacg
    16381 gtagttatgt ttggtatgat gtacttgaat gtctatgcgc tcgatcatat cttcttcagt
    16441 cgtacacgag tattcatggc gttgatgatg ggtgcagtca tggcagtcat catgctgttg
    16501 ttcatgtgga aaatgtatga aaacaaaaag ataaatgtta tgatcttagc cgctagtctt
    16561 cttgtttttg ccggttcctt gtttatggta cgaagccaaa ctactgtcaa cgacacagct
    16621 tggatgaagg ccatgattcc tcatcattct atcgctatcc tggcaagtaa acgagctgat
    16681 ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca agaagaagag
    16741 atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac cattatccgt ccaaataaaa
    16801 aaagaggatg ttccagaagt tatcagcttc aagaaataag aagaagctac ttctggaacg
    16861 ccgtgaataa ggggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    16921 aaatagcttc tcctattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa
    16981 caccgtttat taagaggttg taaaatcagc gttctgacct gagtattaga gagacaaatg
    17041 gaaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga
    17101 acaccgttta tcgagaggtt gtgaaaaagt cgcttagttt cgcgtattaa gaaaaatttt
    17161 gtgaaatggc tttccacatt tttgtataaa tttggcttaa taccgagaaa ctggcttttg
    17221 aataccgttt attcgtaaaa gggattgtga caaaagtctt gtcacaatcc ctttttatgt
    17281 ttttattgtt ctaattgcgc tgtcttgctt actgcttctt gcggtttcac aaattttttt
    17341 gagattcccc aacctgtgaa ggcactgacc atcgaaaaca caggagaaag caaactgaaa
    17401 aatacgaatg gcaagtaagt gagtgtagga ataccaaatg tacttgctac gaaacttccg
    17461 gcaattcccc aaggcactaa gtagttgatt actgttccac catcttctag cgttctacct
    17521 aatacagttg gatcaaatcc tgctttttca taagtttctt taaatgcatt accaggcaga
    17581 ataacagata ggaattgttc accaataaaa atatttactc cgatacttgt catcaatgtt
    17641 gtaaaaatca aatttggtgt gttcgtcatc tttcttgata ctcgttccat cacggttgag
    17701 atcaagccaa attcgatcaa caatccacct aatgacaaag ttagaataat caatgacaca
    17761 gtaggcatca tactttcgat tcctccgcgg ctaagcagtg aatcgatttg ttggtttcca
    17821 gtatttgaaa caaaccctga ttcgatgacc ccagccaact tcgttaagga agtttgtggg
    17881 ttttgaataa aaatcattcc tactgctaca gcgatattca acaaaatcgt taaaattgcc
    17941 ggcacctttt tccatgcaca cagtaacatt aaaaataaag gaatcagtga ccagattgaa
    18001 atagtaaagt ttgcttctag aacatcagtc acttcacgaa tagatgaaag acttgcggct
    18061 ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa caacaaatgc cgggacagtt
    18121 gaccacatca tatgcttgat gtgtttgaac aaatcggcat caacaatagc tgctgctaga
    18181 tttgttgatt cggataatgg agacatctta tctccaaaaa cggcacctga aacaatagct
    18241 ccaacaacca gcgctggatt taagcctagt gttgtcccaa taccgaaaaa tgcaatccca
    18301 atcgttgaca taacagtaaa ggcactccct acagcgctac ctacgattgc acacacaaca
    18361 aatacagaag ggacaaacca ctggacactg atcaatttga acccaatgac cattagagtt
    18421 ggaatgatcc ctgcttgtat ccatacagca atcaatgaac cgactaaaat aaaaataaag
    18481 attgggatga ttcctggttt gatgccattg atgattcctt cgtgcagcca atctgtcggg
    18541 tatcttttta tcactgcata caacatgacc attccgatcg ctgctaagat cgtgatatta
    18601 ggtgagacct ttaatccgac aacgccaatc gcaatgccta aaacaataac cattaataca
    18661 agtgttgctt ctaaaaaact tggtgttcct ttttttccca tgataatctc tcctttttta
    18721 gttgttctta tgttctaaaa aagttaagat atcggattac tccgccgcaa ttcatccgcg
    18781 attcctcctt tataaaaata aaaagacctg ttttaaacta aggacgaatg actccgcggt
    18841 accaccttaa ttaaaaacag atctgttttt cactttcaaa cttttattca aatgtatgga
    18901 ctttccatgt aattcgtcaa ttaggcttga tcggttcaca gcgaccaccg actttctgaa
    18961 caaaacacct aaccgctact gcgtgaaaag ttgacttgta cgaagtttac cttccataag
    19021 atcatttgtc aatgaaaaag aaaatatttt ttcatttttc tgaaaattat ttttataact
    19081 ttcacacaca agattatttt ttactagaaa gtagcgccct cttgtttttt tcttgctact
    19141 atttcttgat gttcttgctt gattctttct aatagctttg gctctttcaa cagatcaaga
    19201 gctgtattgg cgagtagttc tgctgcaatc gcaatagaag caaggccttt gtcactttta
    19261 gcagcagctt tgaattcttc tgtgtgtccg gcaatatatg tctcagagat tgctaccgta
    19321 ggctggattg ttggtatgac ttggctgaca tttcctacat cagaagaacc gagacttgtt
    19381 ctttgttccg tttctatttc ttcatctgga acacctgctt cttttgcatg ctggaagaat
    19441 aagtcatcaa aagaaggagt aacgatgaca tcatcaactg cattttggaa caatccaaac
    19501 tcaaaagtcg tgcccgtact tatcgcagca cctcgtacga tattttccac tttttcatat
    19561 actttatcta atgtttgccg atttcctgca cgtaaataga aacgtccagc agcgtaatct
    19621 ggaatgacgt tggcagctac tcctccgtct gtaatgatcc catggatatt gacatctttt
    19681 ggcaattgca agcgtagagc gttgattcca ttaaacactt ggatcaatgc ttccaaagca
    19741 tttactcctt tttcaggtgc tgctgctgca tgggaagcga caccgtaaaa tttgatgtcg
    19801 actggatcat tcgctaaaga ttctgtcgtt ttcccataac gaaaagctgg atggacgcat
    19861 aacgctacat cgacatcctc aaaaaaacct tctcgaacaa agctgccttt agctgatccg
    19921 ttctcgcccc cttcttcccc tggggtgcca tatacacgga tttctccacc agtttcatca
    19981 atgaattgct tcactatgct ggcggctaat acggagtaag taccaaacaa attatgcccg
    20041 catgcatgac caatacctgg taaagcatcg aattctgcca aaaaggcaat tgtgggaccg
    20101 ggtttattgg aaatataacg tgcatcaaag gctgttcggt gtccagctac ttctttttct
    20161 acccaaaacc tttctttttc taattgttta atcaacgctg ctgaggaata aaactcatag
    20221 ttacttactt caggatgttc gtggatatct agagcaagtg cttgatattc tggtaaacga
    20281 tattggatta gatccttcaa ttggtttgtt gagattgttg ttgtcataaa aaattcctcc
    20341 tatgctagtc atttttctta ttttctcccg aatgtttccc atgcaggcac gcttgatcct
    20401 tttgatgttt tctcgattac tttgactgtg tcttctgttt gataagcgtc tactattttt
    20461 tgatacgttt tattgttttc atcttctttt cttgcaacga tgatattcac ataaggacgt
    20521 gcatggtcat ccactggttc taagaagatg gcatcagaag caggattaaa tccagcatct
    20581 acagctacac cgctattgat cactgaaatg tctacatctg acaaagcacg tgctgtttgg
    20641 gaagcatcta attcagaaat gtctagattt tttctattct cggtaatatc agaaacagtg
    20701 ggcgcttgtc cttttgcctt atccactttg atcaatccag cagtttgcaa taacagaaga
    20761 gctctgccgc catttgtaac atcattaggg attgctactt ttgctccgtc tttgatatca
    20821 gctacatcct tcaaagtgtt agaataaatt cccagcggtg cattgacagt atttccgatc
    20881 gatactaaat cagttccgtg ttctttattg taattgtcta aaaagaattg atgttggaaa
    20941 gaattcaaat caatcgatcc atctgctaaa gccacatttg gttgattgta atctgtaaat
    21001 ttcacgtatt ccaaattaat gttctccttt ttcaaacggt ctttcacgtt gtcccataca
    21061 tctgtatctt ctccaataat ccctagtttt acgatagttt cttttttgtc agttgtatct
    21121 gcttttcctt ggctacaccc tgccaaccaa ataattgcta caaatgtaat gactacaaaa
    21181 cgaataattt ttttcatttt ttctcctcca ctttcaatgt gtaatttttt taatcagttg
    21241 attactgata aattgactta aaaaaactaa gattagaatc aataatgtag ccacaatcgt
    21301 tacatctgtt tggaatcgat tataccctct cgatatagca agattcccta atccgcctgc
    21361 acctactgct ccagccatgg tagtcagtcc gatcacgttg atgatagtca acgccgagac
    21421 ccgaatgata ccaggcaaac cttctaacaa atacacacgt aaaatgatac cgattggact
    21481 ggttcccatc gcttctgccg cttcgatcac cccaggatca acttctagca gtgcgttctc
    21541 gatttgacga gcaaaaaacg gaacaactcc aaaaatcaat ggcactaaag ctgctttcgc
    21601 accaatcgtc gttccaacta agaaacgagt agtcaatgct aaaagagaaa gcaagataat
    21661 gaaagggatg gaacgaatga tattgatcag cttatcaagt atttcaaata gcagacggtt
    21721 agctagtaat ccattagggc gtgtcactac taacgtcaca cctaagatga ttccaatcat
    21781 cccgccaatg aatgctgtcc aaaaagacat atatagtgtt tgcttcgtcg cttcgacaaa
    21841 ttcatccgga atcaaataaa cattgggcaa atatgtttta aagaaatcag tcattttatc
    21901 ttcctccttc gatcactttg atacgactat ctaaatcgtt tttttgtaaa atatttattt
    21961 ttacaccaga tttgatcaaa taatccaatg cttttttccg ctgtttggtt tctccagcta
    22021 aagtaacgat caaactccct aacggcgttt cttgtaataa ctctacattg ccatataaaa
    22081 tattcgctgt tacttgatat ttgctgtaca gatgagcgat tagaggttcg ttcgtctgat
    22141 ttccaatata acttaattcc accagccatt tattggcgat ctgatcggca aaagcagaat
    22201 gggcaatgat cgtttctaat gcttggtcaa gatgagtagc tgttcgaata aaatctttgg
    22261 tcaattcttc ttcaggattg ctgaagatct ggatgctgct acctttttca acgattcttc
    22321 catcttccat aaccgctacc ttgttgcaga tttctttgac aacttgcatc tcatgagtga
    22381 tcaggacgat tgttaaacct aactggcgat tcaatttttt taacaatgca aggatctgaa
    22441 gggtcgtttt cggatcaagt gcactggttg cttcatcaca taataatacc ttaggttcat
    22501 tcgccaaagc tcgtgcgatt gctactcgtt gtttttgtcc ccccgacagt tgtttaggat
    22561 aagcagatgc tttttcttcc aaaccgacaa gttccagtag ttcattcact ttttgacgac
    22621 gttcctgttt cgattttcca gaatacttta aagaaaaatc aacgttgtca aaaatagttc
    22681 ggctttccat tagattgaaa tgctgaaaga tcataccgat ggcctttctt tcttttcgca
    22741 attctttggg agagagctga ctcagattcg tctgattgac aatgacttct cctgctgttg
    22801 gtttctgcag caaattcatc acacgaacta acgtactttt ccctgcacct gagtaaccga
    22861 cgattccata aacatcgcct ttttcaatcg ttaaatctac agaatctact gcctgtatct
    22921 ctttttcttt ttgtttgaaa catacagaaa catctcgcaa ttcaatcatt aaaattcctc
    22981 cttatctaaa aaaagcgtcc tcaagaaaat gaaaatgtca ttttcttgag gacgcttttt
    23041 tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca actcctcttc aagtacgatc
    23101 atactctagc aagatatcgg tcgcagccgt catcgcctta catctatgct cgggatgaat
    23161 tgctcgatgg ccattttcca atagtcgttc cttgcttctt ctcagctacc gaagctctct
    23221 gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa taatattcaa aaaaaaagtc
    23281 cttttactaa cataaactat gtcagtaaaa ggacgacgta tcgcggtacc accttttttc
    23341 ggaagtttcc ttccctcaag cccaacatct caaaaagaga gattggcgtt cgttttaacg
    23401 gtcgaacagt tccgaatttg cctacttatc gacaaattat tctccaaggc cattttcagc
    23461 ttttcttact tgactcttct caccagccag agcctctctg caaagtttcc aaacttactt
    23521 tccttttcaa cgaaatcgat attttgttgt tgggattaat ctacatgagg aaaactgaaa
    23581 agtcaagtac gaaattcaaa ttattttttc tatatttatt tttctgtttt tttatttaac
    23641 atttcaataa cagttcgaag atcttttcct tccgcaagaa gtttcaattt ttccttttct
    23701 tcgacggtat aatttcggac aaaactggaa tcaggtatca aaacaacgcc taatcctgct
    23761 cgtgaagcag ctgttgctcc tactaaagaa tcttccactg caaaggctga actagcttct
    23821 aatcctgttc gttccaaagc tgtcagataa ggaatcggtg aaggcttgtt ctccgacact
    23881 tcgtctccga atgtaaacgt gtccaagtaa tcagctaatt taaagtgatc caagatatct
    23941 gttgctcttt tcgtaacagt ggtcgtagca atgcctagtt tcatatgacg ttcttttaag
    24001 tctgccaata tttcttttgc atatggtttt aagtggatgg ctccgcttgt cagctgttga
    24061 taaaaatatt cttcacgctt cgctcggact tcctgtactt tttcagggct acctgctgct
    24121 ttcactaaaa tatcaaaact ttgtttccag ttttttccgc cccaagcgtc aacgatttct
    24181 tttgggatat ggatatcaaa ttgctgaaat ccccaaagcc agccatcacg atacgttttt
    24241 tctgtgtcaa ctaacaaacc atctaaatca aatattgcac ctttcatcta tacattaatc
    24301 ctttccaaaa caatacacat agtttagcac gactggtcca aaaagaacag aagcaaagaa
    24361 ccagccttct cgtttataag agttcttgga atcgacggat atagattttc ttcacagctg
    24421 tcacaagcgc cagataagcg atgactgtag caaataacca taaccagtaa ttgccaggaa
    24481 gtggcatcaa gcctaattca gcaccaaaac ttgtaaaagg aaggacagaa ccgacaataa
    24541 ttcctaatgt agtgataaca aacatggcaa atgttgcgtt gctttggata aatggtactt
    24601 tcggtgtacg cagtgcatgg agcactaagg tttgtgtcca tagtgattcg acaaaccatc
    24661 ctgcatggaa taatgcgatg aaaatcactc gctgtgaact atccaatgta tggaaacttc
    24721 cgcccactac agcaggacaa ataacaaaat acatcaacaa gtaagtagta atatcaaata
    24781 tcgaacttgt tggaccaaag taaaccatga attccccaat actagatgca ttccattttt
    24841 ttggctcgtc caaatattct ttatccatcc gatcccatgg caaggaaatg caggaaacat
    24901 catagatcag attcaaaaac aaaagttgta aaggcagcat tggtaaaaat ggcaggaacg
    24961 tacttgcaat cagcacggaa aacatattgc caaaattaga acttgctgta gccttgatat
    25021 atttcatgat attcccaaaa gtttctcgtc cagaaagaag gcctctttcc aaaatcgtca
    25081 aatctttttc taaaaggatc acgtctgcag attctttcgc aatatccaca gctgtatcta
    25141 cagaaatacc gatatcagcc actttcattg caggagcgtc attgatacca tctcctaaaa
    25201 atccgacagt atgaccattt ttacgtaaag ctgtcgtcaa acgtgctttt tgctgaggat
    25261 tcaattttac gaatacttca tggtcttcag ctacttgtgc taattcatga tcactcattt
    25321 ccgagatttt ctcaccggta accaattcat tgatctctag acctacttga cggcacactg
    25381 aacgtgtaac taattcgttg tccccagtca gaacttttac agctacacca tgttctttca
    25441 gagctttcaa cgcttgtttc gttgtttctt ttggtggatc aaggaaagca agataaccaa
    25501 tcaaaaccat gtctgactca tctttgactg aaaattctcc aacgacgctt ggattggtct
    25561 tttgtgcgac accaatcact cgtaagccat cttcattaag tgactgtact tttgcaagta
    25621 cggcttcttt tcgttctttt gttaatggtg taacagtacc gccaagatca ataaatttag
    25681 aaatgttcag catttcttca atcgcacctt ttgtgatcat ttgtgtttta ccagctggat
    25741 cagcaaccac aacactcata cgacgacgtt caaaatcaaa aggaatttca tccacttttt
    25801 gataattgat cttatctgta tctaaagttt ctgttgcttc atcaatgata gccacatcta
    25861 acaaattttt cagccctgtt tgatagtagc tatttagata cgcatggcgc aaaacacgat
    25921 catcttcatt tccttcacaa tccatatgat attcaagaat gattttatct tgtgtcaatg
    25981 tccccgtttt atccgtacat aacacatcga ttgcaccaaa gttctggatg gcattcaaat
    26041 ttttgatcac tgttcctttt ttggccatca cacttgctcc tttgaccaaa ttcgtcgtaa
    26101 cgatcattgg aagcatttca ggtgttagac caactgctac agatagtcca aaaagaaaag
    26161 cttctagcca atcccctttt gtaagcccat tgatgacgat aactgctgga gccatcaatg
    26221 acataaattt gatcagtagc atggatgttt tatggatgcc taattcaaag cttgattgaa
    26281 ttggtttttc agatagcgtt tctgctacat gaccaaatag tgtttcattt ccaacagaaa
    26341 caatgattcc ttccgcactt ccactgatga cattacttcc cataaagaca agattttcgt
    26401 aacttgtttc gctttgctga tcgtcttcac atactttcgc atgcttttct actggatagc
    26461 tttcccctgt caatgctgct tgggaaatga ataaatcctt tgcggacaat aaacgcatat
    26521 ccgctggtat catatcccct gctgaaagcc gaacaagatc tccacagacg atttcttcca
    26581 taggaatctc tgtgtacttt ccattacgtt ttacagctgc cgttactttc acaagtgatt
    26641 tcagtttttc agccgcttga ttggaacgta cggactgaat caacgtcatt gtcccgctga
    26701 cgataaccat agtgaagata atggctacac cgaaaagatc tttatctcca ggagctgcta
    26761 atacatattc cgtgatgaat gaaataattc ctaatgcgat caacactaat gtaaatggcg
    26821 tgatgtatgc tttcaatatt tctacaatga atggtgtttt tttgccatat gaaatcgcat
    26881 tttcaccgta attttctctt gaacgttcga tttgttcttc tgtcaatcca tttgctgatg
    26941 catggaatag ttcaaataat tcttgtttcg tttttccagc aaaagctgca tagatttttt
    27001 gtgtatcttt ctttttttcc atctcaatct ctcctttgtg tccaaaagac agctgttctc
    27061 gctgcttttg gtctttagct attcatcgga tcgatagaga aaaaactgaa aggggctgag
    27121 aacagataga taatagccct ggcctttcct ctagatcctt gtttttcgaa tgactgtcat
    27181 ccatttgttc tcatctcctt tactaagtaa ttttaattca tgacttccaa tcatttttcg
    27241 aatagttatt tttccaatta gaccacctcc tttttatttt tcaccgatgg ggatttgcgg
    27301 caacaaaaaa acctctcgcc aaatactcag cgaaaggttt gaaaaagcat attaaaaaaa
    27361 gcttctcaga tctcatcgtt gagttttgac actgtacaac gtaaaaagca gatacctgct
    27421 tttagctacc ttatgaaaag ccttatctcg acaattcctg ttctacccat tggcgtctct
    27481 cgacgttttt gggcagtagc ctatgtttgt ataggagcct cacctaacaa atcttattcg
    27541 gttttccttc gttaatataa caactaaaaa agaggatgtc aaatcaattt tccatgtttt
    27601 tcatactttt tttaaaatac acacgttata ttcctgtctc acgcggtttt atttgtctca
    27661 ttttccacct cccgctataa tagaaatata gtaggaaacg aggtgctgtt atggaaagaa
    27721 cacttgttat tatcaagcca gacggcgtga gacgccattt agtcggaagc atcatccaac
    27781 gttttgaagc aaagggactg gccattgctg aaatgaagtt tgaaactatg actcctgaat
    27841 tagcaaaaga acattatcag cacttgactg agcgcagttt ttttgatgaa ctgatcgatt
    27901 acatgacttc cggtcctgtc gtttatcttg tgctggtcgg cgaggaagtg atcgatatcg
    27961 tccgtaagat ggttggagca acaaaagctg ccgatgctgt tccaggtacg atccgaggag
    28021 actacgcgct gccggggact gaaaacatca ttcacgcttc tgattcccgt gatgctgcgg
    28081 taaaagagat cgcccgcttt ttcccgccat tcgatataaa caaaccgtct gaaaaagcgg
    28141 cgaagtgata caaattctaa actgctggtc tagttgctgg gttcagcaat ggccagcagt
    28201 tttttctcta aggacctccg ttgattcttt cctaacatat attcgagcac tgtgaagaat
    28261 tcttggtagt ctttcgtctt ttcttcccct aaagcagcag aaatcaatct ttgataataa
    28321 tctgccatca atgtttcata gatcactgta cttctttctt tatgtgctaa gctccgtttc
    28381 atatcttcat tcacactacg tgcctttttc aaccgaccat tttctatata aaacgaagtc
    28441 aggttcatta aaaaagtaaa gagatactgc tcatgattgt tcaactgtgc taatcgtcgc
    28501 attctttttt tgattttcaa ataattgttc tcaatgaacc gatcactgaa tatccaagga
    28561 aaatcagcta gaatagatag ttcaaattga ccatagtctg gtatctgcat caagtattct
    28621 tcaatgattc ggcattcata acgtaaaaat tcaaattctg tctcagatac ttctgttttt
    28681 tcgaccatgt gaagattcaa aaccaaataa tacagatctt ctgttttctt aaaatgccta
    28741 cggttattgc tgatctcttt ataaggatca aagctgtctt cttgtacccg atctagcatt
    28801 cgtctttttt tctttaactt tattggttca tccagctggt ttcgcatcca ttcatattct
    28861 gcaagtgtca tgttcattcg ctctaaaaat gatctcaatg ttacaaatga catttttttc
    28921 actgaatttg tctcaatccc gactagagaa gtcttaaaac agattccttc cgctaattgg
    28981 gactgtgtca acttcttgtt gactcgaagt ttcttgatca attcaccatc atacataata
    29041 gttctccttt ttaatttaaa tttttccaca ttcaataaat agatacgtta aaaaggaaaa
    29101 atacacgaaa aaaataaaaa aatgtttttt tcataaaaaa taccaacctt caagcattag
    29161 aatgttgtct aactactgaa ggttggtttg attgtttatt gattattttt ttgctgttca
    29221 acgtctgttc ttgttactgg atataagttg gttgttgttt tattaccatt ttttgaaaag
    29281 acacttgtcg aatgttcgcc tttttgcttt tcgattgctt cattacgttc cgcttcattt
    29341 ttatagtcat attttttcgg atcaatcgtc tcaaatccgt ttggcacata aaaccgcaag
    29401 agattttcct gattcagctt atctgaaaga cttaacgcag tcttgacttt ttcctgatct
    29461 ttggagattt cctcatcgat tcctgctgct ttcgtatcga tgatttctcc tgtctgatta
    29521 ttgtaagctt ttcctcctag gacggtgtat ttgggtgtca caaagttccc atttcgaaaa
    29581 gcaactactt gatcatgctg agaagataat aagtctgtac caaaatgaag ataatcttta
    29641 tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat cgatttctcc accgtattca
    29701 tgattgactg tccctttagt ataaccaggc atatggatca tcaatggcac tcgttgcatc
    29761 tgtgcgttgt caaattcatc ccatgtatct ggatctttac caagagcgct tgctaaatct
    29821 ttattatctg tattcgagat cccaaaatga tccccgtaaa tcacaaagat tgaattttgg
    29881 taaataccag attttttcaa atacgtaaaa aattgttcca atgattgatc cagataatgt
    29941 gccgttcgaa cataattatc tactgtgcta ttgccagtat tcaaagatgg aaagtcgaaa
    30001 ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg aaagaaattt cgtgtaaaac
    30061 ggctgttgta gatgctcaag atattgggca gattctctga ataaatcttt atctaatatc
    30121 ccataaccta acgtttcgtc ggattcatca aaataagatc gatcaaagaa attgtcataa
    30181 cctaagtttt tatatacatg atctctattc caaaaactag ctacattccc atggaaaaca
    30241 gcgcttgtat agccttgctt ttgtccaagg atcgctggtg cggcttgaaa gacattgtct
    30301 gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa atgtgccagt ttccaacata
    30361 ttttcagcat cacttgtctt tccctgtcct acttcatgaa agaaattgtc aaagctgatc
    30421 gttgcttgat tctggaaaat actatttaaa aacggtgtga cttcttgtcc gtctaccttc
    30481 atattaatca aaaactgctg gaaactttct aaatggagaa cgatcacatt tttcccttta
    30541 gcgatcccaa atgtttcggg atttggctct gcataatgtt tctttgtata atctaaaact
    30601 tcatcaatcc cattactgct tgcatgcgcg cgaacactgc tagtcattcc tgttttgatt
    30661 ccgtcataaa tagtatacgc atcgattcct agatatttga caatatatga acggtcaaat
    30721 gttcgctgca gcaattgtgg acggttggct tcgctcagcg ctagatttcc tgaaaaaata
    30781 agccctgaaa gacaggtaac agcgatagcc atcggtttgc cgacagggtt gcttttgata
    30841 ggtattttct tgaccttcaa ccaaaccagt agcccaatga acacagcgat atccagccag
    30901 tagatgacat catggggctt catcaaagaa aaggagcttc ctgacaatcc ttgcgacact
    30961 ttcgaaaagc ctaatacaga tttgactgta atgaaatctg tgaattcccg ataaaaaata
    31021 atattcaagt acaacaaaag cgtatttaaa atatcaatga tcaaaagaat agctaacgct
    31081 ggctttggct ttttaatata caaagaaagg ccaaaaaaca ataaggtagt cgcaattggg
    31141 ttgatccaca agataaagta ctggatcgta ccttccacac ctaatgaaaa atctgcatag
    31201 taggcaatga tcgtcttcag ccagaaaaag aaaacagctg tcaaaacgat tgctaaacga
    31261 ttttggcaca attttttaag ttgtgtcacg tatatcactt ctctctattc acattttttc
    31321 ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt gttatcttaa gattttttta
    31381 ttctttctaa agattattcc gtccactttt cacagcataa aaccgctgcg atgtaagcga
    31441 ttaaactaat gaagagataa aagtcttgtg gtgaccgctt cactccacgg tattcaaaag
    31501 ccagtttctc ggtattcgta caaaaaaaga ggctgtgaca aaattcttgt cgcagctcct
    31561 tatttcttga tgcaggacag ctttttcaca acttcttcct taaacggtgt tctcctgcca
    31621 actcctcggc cacaagtcac aatatttata aaacatgaga agctgttttt tgaaattgtt
    31681 ccttgtggct cagagttaaa cggcaggctc aagacctctt gatacacggt gttcaatcag
    31741 ctgaccctcg gtattagatt atgtcaggac gctaatttta caacctcttg gtaaacggtg
    31801 ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatataa ggagctattt
    31861 tttgaaattg ctccttgtgg ctcagagtta aacggcaggc tcaagacctc ttgatacacg
    31921 gtgttcaatc agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
    31981 ttgccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct ctgtttttca
    32041 ttgtccatag gattgatatt gtccttgtga tgactcattc gtaataattg aggcttgatt
    32101 ttcttcactt cccgttatcg tttgtccact gccagaatct gaaggacttg ttcctgtcga
    32161 ctcgtttttt tgagaagaga cagttgtttt cttgacttgt tcatttgtct ttttccattc
    32221 cgctaaacga tcggcgacca cttttttgtc tactttttca gaataagcgg tgatcacttt
    32281 tccatttttc aaacgggcta ctttgtcgcc atcatgagtc aaaagcaaaa cggtcaaatg
    32341 cttgtcttta aattgatatt gtctgagaga aatcgcacta agtacgtcat cgatattaat
    32401 aaattgattt tcttcttttc cttttggcaa ataaatgtcc aattcgcctt tttcgtaatt
    32461 ggcagttatt ttatactccg cccaccaata agttgagaat tcttttttga aattatctac
    32521 tgattctttg cttgctacga ttttttctgt ttctttctga gcaagatctg ctgtcatttt
    32581 tcctgaaaat gctgcggata aaataagcag taagaaaaaa ccgaataacc aaaagagaag
    32641 aaacggtctt tttttcctca tgctttcctc cactcctatt taattttgac tattctatta
    32701 tatacaaaaa atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat aattgaactt
    32761 ctttttcaca aaatggtaag aatggagaat ttcacgcatt tgctctcaac tattctttct
    32821 taagcctcaa atccgttttt atctgatacg tctttgaacc aatgacccga ttttttgatt
    32881 gttctttttc catcttgtgc taagtctact gaaataaagc catatcgatt cttatatgca
    32941 ttggtccacg accagttatc catacatgtc cacatatgat agcctaaaca attggcacct
    33001 tcttgaatgg cttgatggac atatttcaga tgagattgga caaattcgat ccgataatca
    33061 tcgtggatca tgccatcttc tttgataaat cgtgcttcgt tttctacacc cataccgttt
    33121 tcagaaataa agcagtcgat atttccataa tgatctcgta cattgatcaa aatatcatag
    33181 attccttgtt catagatctc ccatccgcga taaggattca ttttacgacc aggcatttca
    33241 taattgtcga aataatcctc aggcattgga ccgtttttag tatcgattgg tgtttctttt
    33301 gcttttaccc ggcgtggctg gtaataattg atacctaaaa gatctactgt attttctttg
    33361 ataaccgtca aatcctctgc atggatcaca ggcatgagat caagttcttt tactatctca
    33421 atcaattcac ttggaaactc gccttttaca gctggatcaa ggaatgaacg attgaagaac
    33481 gcatctgcta tttgcgctgc tttaacatct tcagggtttg attcgtctct tggatagctt
    33541 ggtgtcagat tcaagatgat accaataaca ccaccttgct ccatttcatg gtaagatttg
    33601 atggctaacg ctgaagccaa cgcttcatga tagccaactt ggatcgcttt tttcatatct
    33661 gactcttctg gataatgcca tcgatataaa taacctgctt cgactggaac gattggttca
    33721 ttatgagtaa accatttttt tactttcgaa ccaaacaatt caaaacaact tttggcatat
    33781 ttttcatagg cttcaaccgt ttctcggttc aaccatccgc ctttttcttg cagatacatc
    33841 ggcatatcga aatgatacag attcacaaag ggttcgatac cgttttcaat caactcatca
    33901 aaataacgat gatagaaacg aacggcttct tcgttgattg ttccagattc atcaggtatc
    33961 aaacggctcc attggataga tgttcgaaat gaattatgcc cagtctgttt catcaactgt
    34021 acatcctctt gatacttcgt ataaacttgc gaggtttttt caggtcctac ttgatcaaag
    34081 aacttttgcg gttctttttt gtaccaataa tcaaaaatgt tctctccttt tccatcgcct
    34141 gaaatccttc cttctgtttg tgggccactt gctgcagaac cccaccaaaa atttgctgga
    34201 aattgatatt ttttcatcaa agcacctctt tttctttttt agttcgtttt agttgattcc
    34261 atgtatgata ttttccatca caatcacttt ttcattttct tcctcttctt ttttgattcg
    34321 ttttaacagc tgttctgctg ctcgttcccc gattttatct gttggtggcg tgatcgtgat
    34381 tttcggaagg atactcagat cgtttttatc taaataatca tagtgactga acgcaatatc
    34441 cttcccgatt ctgacacctt cttgattgaa acattcgatg gcaccaatcg acatattgta
    34501 attgctgaca aaaatagcag ttgccccttt ttctaataat gcttttgccc cgtaatagcc
    34561 gctttttcta gaatagtctc ctgtaaagac ctctaattgg atcgatggat cattttggac
    34621 cgcttctttg gctcctgcta aacgttctct tgctacgtag tctgtttgag tggcagcaat
    34681 aaatccgatt tttttatgtc cttgttcacc atgatgagcg atcacttttg ctacactttc
    34741 tcgatcattc actaatattg tatcaatacc tgctggtcct tctggacaat tcaatgcgat
    34801 aactggaata ctcatttttg tgagcatttc aaatccagac cactcttctt cggataagaa
    34861 gacaatcagc ccatcgatca catgcttcat caggtattca attttttgtt caattttttc
    34921 tggctgatcg gcaaacccgc ttaataataa gctataccca ttttcttcag ctactctttc
    34981 gatccctgag acaacttccg acccaaaacg gctggaaatc ccgtttatca aaagtccaat
    35041 agagaagctt cgattattct tcaacccttt agcaatgatg ttttctttat agtccaacgc
    35101 tgaaatcgct tctgctattt ttttcatgtt cttttccttc agttgctgac cattcaagta
    35161 acgggaaact gttcccactg aaacaccagc catcttagcc acatcacgga tcgatgccat
    35221 acattctcac tcctgccagt tacatttgat ttctctttct tgttgttcga ttatgatgac
    35281 cgcattcttt gcatagtgtt gcttgtcatc aatatccata taactaaagt cttcagtaga
    35341 gcaacttcct ctcactatta attatactta aacaagtatt tgaaccggtt catttttaat
    35401 atagcatttt tttaaaagat tgcaacactt ttttaaaacg ctttctgaaa gtggcggatt
    35461 ttttgccttt ttaaaatttt attctaaaat ttactctatt tgtgggtgtt ttgtgcgttt
    35521 ttctcttcca tccgtttgaa gttttacatt ccaatggtat aatttcttta tcctatcaaa
    35581 tgggggaagt attttgaaga aattaatcgt actatttatt ttgtctgcgg gacttttgac
    35641 attgactggc tgtgatttgt ttggcggctc tgaccaatcc tcggacgaaa caaccgattc
    35701 ttctgtctta aaagcggaaa aagcagatac caccaacagt gtatatacgc taaaagtacg
    35761 taaagatatg gatttatccg cgggacagtt gacatttaca gataaaaaaa tggaatggac
    35821 acgcacgtat cagacacaag gaactgattc ttctgaatca tctgaagaaa aaacagtgct
    35881 gacagatatc aagatcacta ccaaagatga cgaatatatt atttcaggaa aagaaaataa
    35941 aaaagaaaca acagtcatat ttacaaaaat cggtaattac cgtttgaagg atgaggatgg
    36001 caacatctac agtctatagt gccgtctgtt ggatcttctt ttgtttctcc ccttcttttc
    36061 tgctatagta aagaagcaag atgattgtaa aggagacgaa atggatgatc gatagtcctg
    36121 attattgtac atgtaataac aacaaaaata tccatacaga atttaatgac caaggaagct
    36181 tgagcatatg cgacaactgt cacaaactag tcaaagattc atttaaaaca tttgaatctt
    36241 atcctgttca aaattatgta aaaagtagaa gaagataaac aaatccaagc aaaactcact
    36301 atggctgatt tttatgaact tcaaactcag cttcttcctt tgatcaagct tcatcagtaa
    36361 aacaaacagt ggatcatttc agtttacaaa tcaaagagta aagatttatt tatttcaaat
    36421 atttgaatat aaaataacca aacgatagaa aggcttattc tacatcacgt ag
//
LOCUS       DAZKOR010000018        35005 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000018 DAZKOR010000000
VERSION     DAZKOR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.018"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..435)
                     /locus_tag="ACG8ZM_000831"
     CDS             complement(<1..435)
                     /locus_tag="ACG8ZM_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491814.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASL"
     gene            complement(730..1065)
                     /locus_tag="ACG8ZM_000832"
     CDS             complement(730..1065)
                     /locus_tag="ACG8ZM_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0491815.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1052..1336)
                     /locus_tag="ACG8ZM_000833"
     CDS             complement(1052..1336)
                     /locus_tag="ACG8ZM_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0491816.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1392..2915)
                     /locus_tag="ACG8ZM_000834"
     CDS             complement(1392..2915)
                     /locus_tag="ACG8ZM_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0491817.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(2908..4083)
                     /locus_tag="ACG8ZM_000835"
     CDS             complement(2908..4083)
                     /locus_tag="ACG8ZM_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0491818.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4087..4272)
                     /locus_tag="ACG8ZM_000836"
     CDS             complement(4087..4272)
                     /locus_tag="ACG8ZM_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491819.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4238..5932)
                     /locus_tag="ACG8ZM_000837"
     CDS             complement(4238..5932)
                     /locus_tag="ACG8ZM_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0491820.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(5929..6402)
                     /locus_tag="ACG8ZM_000838"
     CDS             complement(5929..6402)
                     /locus_tag="ACG8ZM_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0491821.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6471..6626)
                     /locus_tag="ACG8ZM_000839"
     CDS             complement(6471..6626)
                     /locus_tag="ACG8ZM_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFQ0491822.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(6760..7140)
                     /locus_tag="ACG8ZM_000840"
     CDS             complement(6760..7140)
                     /locus_tag="ACG8ZM_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0491823.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7144..7359)
                     /locus_tag="ACG8ZM_000841"
     CDS             complement(7144..7359)
                     /locus_tag="ACG8ZM_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491824.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7362..7769)
                     /locus_tag="ACG8ZM_000842"
     CDS             complement(7362..7769)
                     /locus_tag="ACG8ZM_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFQ0491825.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8035..9510)
                     /locus_tag="ACG8ZM_000843"
     CDS             complement(8035..9510)
                     /locus_tag="ACG8ZM_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFQ0491826.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9500..10348)
                     /locus_tag="ACG8ZM_000844"
     CDS             complement(9500..10348)
                     /locus_tag="ACG8ZM_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFQ0491827.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10385..10747)
                     /locus_tag="ACG8ZM_000845"
     CDS             complement(10385..10747)
                     /locus_tag="ACG8ZM_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491828.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(10748..10906)
                     /locus_tag="ACG8ZM_000846"
     CDS             complement(10748..10906)
                     /locus_tag="ACG8ZM_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491829.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11047..11671)
                     /locus_tag="ACG8ZM_000847"
                     /pseudo
     CDS             complement(11047..11671)
                     /locus_tag="ACG8ZM_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(11668..11979)
                     /locus_tag="ACG8ZM_000848"
     CDS             complement(11668..11979)
                     /locus_tag="ACG8ZM_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491830.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12023..12316)
                     /locus_tag="ACG8ZM_000849"
     CDS             complement(12023..12316)
                     /locus_tag="ACG8ZM_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491831.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12510..13157
                     /locus_tag="ACG8ZM_000850"
     CDS             12510..13157
                     /locus_tag="ACG8ZM_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0491832.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13218..14363
                     /locus_tag="ACG8ZM_000851"
     CDS             13218..14363
                     /locus_tag="ACG8ZM_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0491833.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14444..14749)
                     /locus_tag="ACG8ZM_000852"
     CDS             complement(14444..14749)
                     /locus_tag="ACG8ZM_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0491834.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(14774..15205)
                     /gene="ruvX"
                     /locus_tag="ACG8ZM_000853"
     CDS             complement(14774..15205)
                     /gene="ruvX"
                     /locus_tag="ACG8ZM_000853"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0491835.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15202..15468)
                     /locus_tag="ACG8ZM_000854"
     CDS             complement(15202..15468)
                     /locus_tag="ACG8ZM_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0491836.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(15723..16448)
                     /locus_tag="ACG8ZM_000855"
     CDS             complement(15723..16448)
                     /locus_tag="ACG8ZM_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0491837.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(16809..16879)
                     /locus_tag="ACG8ZM_000856"
     tRNA            complement(16809..16879)
                     /locus_tag="ACG8ZM_000856"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16845..16847),aa:Gly,seq:ccc)
     gene            16996..17748
                     /locus_tag="ACG8ZM_000857"
     CDS             16996..17748
                     /locus_tag="ACG8ZM_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0491838.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            17764..18243
                     /locus_tag="ACG8ZM_000858"
     CDS             17764..18243
                     /locus_tag="ACG8ZM_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0491839.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(18494..20074)
                     /locus_tag="ACG8ZM_000859"
     CDS             complement(18494..20074)
                     /locus_tag="ACG8ZM_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0491840.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20067..20810)
                     /locus_tag="ACG8ZM_000860"
     CDS             complement(20067..20810)
                     /locus_tag="ACG8ZM_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491841.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(20986..21762)
                     /locus_tag="ACG8ZM_000861"
     CDS             complement(20986..21762)
                     /locus_tag="ACG8ZM_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0491842.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22010..22846)
                     /locus_tag="ACG8ZM_000862"
     CDS             complement(22010..22846)
                     /locus_tag="ACG8ZM_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0491843.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(22857..23786)
                     /locus_tag="ACG8ZM_000863"
     CDS             complement(22857..23786)
                     /locus_tag="ACG8ZM_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0491844.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            23978..24331
                     /locus_tag="ACG8ZM_000864"
     CDS             23978..24331
                     /locus_tag="ACG8ZM_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0491845.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(24405..25061)
                     /locus_tag="ACG8ZM_000865"
     CDS             complement(24405..25061)
                     /locus_tag="ACG8ZM_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0491846.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            25287..27221
                     /locus_tag="ACG8ZM_000866"
     CDS             25287..27221
                     /locus_tag="ACG8ZM_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0491847.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(27307..28530)
                     /locus_tag="ACG8ZM_000867"
     CDS             complement(27307..28530)
                     /locus_tag="ACG8ZM_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0491848.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(28524..30395)
                     /locus_tag="ACG8ZM_000868"
     CDS             complement(28524..30395)
                     /locus_tag="ACG8ZM_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0491849.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(30416..31534)
                     /locus_tag="ACG8ZM_000869"
     CDS             complement(30416..31534)
                     /locus_tag="ACG8ZM_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0491850.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(31617..32450)
                     /locus_tag="ACG8ZM_000870"
     CDS             complement(31617..32450)
                     /locus_tag="ACG8ZM_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0491851.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(32443..33360)
                     /locus_tag="ACG8ZM_000871"
     CDS             complement(32443..33360)
                     /locus_tag="ACG8ZM_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0491852.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(33587..34711)
                     /locus_tag="ACG8ZM_000872"
     CDS             complement(33587..34711)
                     /locus_tag="ACG8ZM_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0491853.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            34894..>35005
                     /locus_tag="ACG8ZM_000873"
     CDS             34894..>35005
                     /locus_tag="ACG8ZM_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0491854.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLL"
BASE COUNT        10508 a         7069 c         5712 g        11716 t
ORIGIN      
        1 tagacttgct aaagcataca acacattttc caaatttgct tccttataat ttgaagaacg
       61 accatgtttt actctgttat atattttcca ccaatctggt gtatcttcag gccacccatc
      121 tccaaaaggt tttagtgaag tcaatgttaa agaattcata accctaacac cattatccaa
      181 acacattaat tcgctagatt taataacagc tttaaaatca gttatgcttt tattgctact
      241 gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac tccgaaccaa tcgctagtag
      301 tagtttttga tattcaatag agaatgtttt gaaattattc tcatcgagca tcacaaaatc
      361 tgtggtatta ataaactcat tttctaaatg tagataatac ttccaataaa aatttgtaaa
      421 ctctgctttg tccattaaat cacctctttt gaattataac aaaaaaaaca cgtccacaac
      481 agacgtgttc tttgatttct acacaatcag cttgccaaaa ccatccaatc agtcctagca
      541 tgcctgtatt aaaattgaac gttcatcatc tatcatcttt cttagtccag catgtttgca
      601 accacaccta caattttcag tccaacaata gatttcgact agctaagtct tgcaaaattc
      661 aggcagctaa cccttcattt actaccttaa ttatactaaa tacccacaac gtttacaagt
      721 gtaatcattc taagaaatct gtttagctat gatcgttgta taatctttaa acgccgttcc
      781 ttcattgatt gaaataattt tataactctt gtttttccaa gctactttca tatcgtttgt
      841 tagttcataa tcttgatcgt atcgaataat aaacgtcaac gttccttcaa gaatggtacc
      901 tacactagca agcacgtctt ttagcatttg tgaacgcaca ttagcccaac attcaaatac
      961 agtttcttca ctatccacca tctgcccatc ttcatctttt acccgttttg tctttttaaa
     1021 cttgactctc tcagtcaatt catttacatt cttaatgatt ggcataataa gcacctctca
     1081 tttgttgtac taatgcttgt accgttactg gtatccgttc gctgctggct tcttgacggt
     1141 tcaaatacca atgttgagta agtaatgaca ccgccaaatc aaattgctga taattatcca
     1201 tatctttatc cgttgcgcta ctatcaatag cactttttat atattcgcct gctgtatcaa
     1261 tcaattgttg aataaagtca tcatcaatag tgtgatcgat ccgcatactt ttttttatgc
     1321 tatccaaaat taccatttta tctctcctaa tgaaaaaaag gcagtgggat aaaccgccgc
     1381 ccaatatttt tctatacttt tggtgttaca actggcgtaa actcaatata cactgaagca
     1441 ttttgatcaa ttttcttata atcgttgcga acgatcactg caagtccttg tgagtagtaa
     1501 tcgaattttt cccattgagt cgttacttga tttctacgag caacaaagat agattgtgca
     1561 atatccccca taatcatagg gaatgttcca gctttaggat tggcaaacaa tgtatctgca
     1621 atcaatacta ctggcatacc gaataatgat ttaccactag gtgctgtcac atcgggttgt
     1681 aaaatgtaac gtccatctga atctttcaat gtatctaggt ggtaatatcc gctttggttt
     1741 agaattacca ttttatttaa tgctgggtct aatgccacat tgtatagttg tttcaaatcg
     1801 tccaacgtag cagccgtttt cttagtgaat gtttttaata aatccatgat atgcttattg
     1861 tcagtgtttt ctaccaattt cgctaactga tctttgacct cttgtacaat attcactgct
     1921 gaatcttcca caacctcatt tgataaggca atcttaccag cacgagtttc tactttataa
     1981 tcaactgaag taaacatttc agcgtcaata tcaccaattt cagcaagttc agcttttgtt
     2041 gctaacactg cttgttggtt agttgctact ggatatttac cttgtccatt tgatactgtt
     2101 tttactgttg catattgagc taagttataa tttgaacgtt tcaaatcaaa gacttcaccg
     2161 attacttctt cgggaacaac tgccgctgca tttacagtag tgactccatc acgcacttcg
     2221 ccttgtgaac ggatatattc ttcataccca cgcaattctg ttttttcgtt atcgattaaa
     2281 gttttttcca tgttattact ctccttttgt gctttagaag tggattctaa gctattgttt
     2341 gtttgttttg cttggttgtt actcataaac gattcgtaag aacgcttatt tacttggaca
     2401 tttgatgaat cataagcggg aacggtcacg acactaattt cattaagtgc tttgatttta
     2461 tctattgatc gttcaattgt gccatcttct tttttgtcga atgaatctaa tccaagcacg
     2521 aacccaaagg acatagaatc aacaacccct tttgagatat tctcatacac atcatttgaa
     2581 taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg tatcatcgac attcaatttc
     2641 aacgtacctg ctttgacact agctagtggc tttgaataat cgtgtccata taataagaac
     2701 acatttgata aatccacctc ttttaatgct tcgggtgtaa tcacttctac aaagccgcct
     2761 aaatcttttg acggttgccc gaatttcaac gcatagcctg aaatagtttt gccgtctgtt
     2821 ggttgttttt cattttcttt ctctactttg ggagaatcgg ctgttagctc agcttcttct
     2881 gtcagccgtt tttccttttc ttcttcatta ttcaactggt gtaactcctt cctgttcttt
     2941 taattgatat ttctccaacg tatctaaata cgtaaagttc aaacttgcta gtaaacgatc
     3001 gccaccatcc atggggggta gccccatttt cgaccgcccc tcattaatcg tgagaagtga
     3061 ccctttgacc tgatccaata cattttttac ttttgtttca ggatcagtct ctagtaactg
     3121 atcggaatta aagcggatat tggtttcaag tttcgtatct aattcactca caaacacatt
     3181 aaagtaatga atcagcgtac tttggatata ctggagatta ctttgaacgg tgctagaatg
     3241 ttcattttca acgcctaaac gctcaatcgg tacaccaaac gccttggcaa tttgttttgt
     3301 gttccaatca ttggaattga taagcttcaa aacatcggtg tttatttcta atgttttgta
     3361 gtccattgtt tcatcaagaa tgatcgttct aagagcatta tctccactac ttgaaccgtt
     3421 ggcttcttca aacttttcac gaattgcgtt ctttgctgat ccgtctaaat ctgacttatg
     3481 aaccttcaaa atacctgacc cactgacacc acgagtaaag aaattgtgca atgtgcggtt
     3541 gccagcttgt tgaattttca gttcatcacg taacgcataa agtggtggta atcctgttaa
     3601 accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta gacttcacac gtcttttttt
     3661 atcaccaatc tgataagaca acgtaccatt atctaattga gtaaccgtta cacttgagtt
     3721 aggtaataag tgaatttctt ctaccttttc accgttgcgt ttaatctctg caaagctatt
     3781 accgtttagc aacatattga ccgccaaagc aaactttaaa tgccaaccat ccatttctga
     3841 attaggtttc tcgtttagta acttcaccag ttcattatca gcttgtggca tattgttttt
     3901 gaccaattga atcggacttg aagcaatatc actagcaata attcgaacgg ctgcaaacac
     3961 atcactattt cttaatgcac gaacgcttgt aaagctggtg gtgtaatcat ccgattggat
     4021 tgataccaca tgttgtagga aaggatctcc cgttgtattt tgtctcgctt taaaaaatgc
     4081 catcttttaa cctcccttct gttctgtatt tagcatgacc gctaccccaa taagcactat
     4141 gcccagcgct aacaagccat agtagacatt tgttagtaag gtgatagcaa ccaacacaca
     4201 caccaatcca agaagtaaaa gaacggtatg aatgtaatta gaaagaaaat tcttcacttg
     4261 tataaaattc attgtctgct ttcgctcctt ccgttgtggt gaagtaatcc attgcaaaca
     4321 cataggcatt gattaatgca gctattggat caattttgtt actgttcttt gatttattaa
     4381 tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt cactgcatgg gtgagaatcg
     4441 tatttttatt gtgaataacg ttgccttcat aaacttgttc tctaaaagtt cgggtcggaa
     4501 cgtttagagt aatcgttcct tgtcttactt ctaacattgg gtagttggct ttttcagctt
     4561 tactgataag cgaattagcg ttatacgggt cataacaaat ccccatacat tccaaatcat
     4621 tttcttgaat caaatcttga ataaactgaa atacttggtc atagtccacg attccacttt
     4681 ctaattgagt aatggaacac tcaccagctt tttctagttc tcgataaggc aaaccgtcgc
     4741 gcttttcttt gtcttgaagc ccatatttgg tggctacaaa gctatgtgaa tcacaataaa
     4801 gttcgccgtt atctagcggt acgatccatg aaacgctagt taaatcatct gttttagata
     4861 aatccactcc gatataaacg ggcttgcctg taatatcttg tggttctact tcggttgcct
     4921 gccagtcttc agcaatcata tagctgtcct cactcgcttg tctccataaa ttgaagtttt
     4981 tcaccaatac agaattaagg ttattttgtt tcaaagcaag gttcacatca tcttgaatgg
     5041 ttggaatcat tacttttttg atttcttcac tttcaaaaat cggattggct ttgatccatt
     5101 tctcttgatc gtgaatttct tcgggatcgt ccaattccca tatcgctata aagtacctgt
     5161 cggcttgttc ttcgcctttt agcacacgat ctaacaataa gtattcctcg tacataggaa
     5221 cgtttaaatc aagcccagcg gtactaataa cacacaataa gccgttcttt tgttgggtca
     5281 ttcctgactt aatgacgttg tacgttttac gagtttttgc ttcgtgccac tcgtcaagaa
     5341 ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc actagccagt gccatagcga
     5401 acgaattaga gggcaagtgc tgaatcttag agttcataat tttcagttgt ggtcttaaaa
     5461 acttactaga cttgaccacg tttctaagtg aattagacag catatcatag cccaatttag
     5521 cttgtttcaa agcgttactt acaaacaata cttgccttgc ttctgctggt tcttgttcca
     5581 taatcaacgc attggcagcc atgccacttg cgatatacgt tttcccattc ttacggctca
     5641 tactggtaaa cgctcgattg aatcgcctgt aattcccttc tttagtacgc cagccataaa
     5701 gactgcctag aatgaatttt tgaaatccta acatcttgat tgctttaccg tccgtagatg
     5761 gtagcaactc aatgaattta atcgctttgg tggcttgttc ttcatcgaac acataaggga
     5821 agctatctga cttagaacgc tccaaatcgc ttatatgacg ctcacacgct tggataattt
     5881 tcttgggtgc aatcagctta cctgatagga ctttctcaat gtaatcattc atgaattcat
     5941 caactctttg aacgggtctt cgggttcatc atttttaacc ttgttcaaag ctagttttga
     6001 tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt ttcaaatcac tcatagcttg
     6061 tgattgaacc cgaaccgctg gattggcttt ctttactccg ttttcgcatg tgataaacgt
     6121 acctgatttt cttatctcgg cttcggctgt tttcattcga gcaaaggcta aacaataggc
     6181 taccaatgaa ccataatctg cttcactcaa tggataatct ttttttaatg ttggtacgat
     6241 acgattccat tcacttctag cgctcgctgc catccagtca gggggcgtga tatttattaa
     6301 tggttgctgc ttgaacaatt cttctctagc gtccacacgg attgcttttt cttcacttga
     6361 tatattgcct tttgtatcgt gtaatatttt agggtgtccc atgttgtcct cctttcagac
     6421 aaaagaaaaa ggaacgtaag caattaagct cacattcctt cgtttttcga tcagaatttt
     6481 tctttatcta atttatctat ataaatgatt tttccgttct gcatttgaat ggtcacttga
     6541 ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt tatcagtaat aacaattgta
     6601 tttttcaata aatctcctac tttcattata tactttttca cttgtgatta caagtgtaat
     6661 cgttatttgt aattaccctt atgccttttc acaacggtta tttgtaccaa aatgagtagg
     6721 catcgattgt cagtaagttt cacactatgc ccccttaact caaagggtac tcttttctcg
     6781 tctttgtctt tcattggctg tcttggaatt atggtgtgca tggcataaag aacgaaaatt
     6841 agtttcatct aaccgtctat tccaatcatc ttttagttca attttgtgat ctacaacatc
     6901 agctttcctt atcaagcctt cttctaggca atcctcacat actggatgat ttaaccgata
     6961 caagtatgaa gcctttcgcc atgttctact ttgatagaat tgaaaatatt taccttcgct
     7021 ttgctttctt tcttcacgct taattgccgt ttgctttgct ttatgcttcc cacaatactt
     7081 ctgcctataa tcaatcaatg ttttacatcc agcatgatta cattgttttt taactgccat
     7141 agattaccaa ccaaagcgat ctcgttcgtc agaatcatct tgttcccatt ttgatttgta
     7201 ttcaatataa ggcgaaacta tcttcaagca cgttccgtct ttaaaattaa ctttagtgat
     7261 tttatcttcc tcaaaactaa taaagtctac atcttcttga gtgtttgaat actcaatgtg
     7321 tttctcacca tcgaagtatc taatactttc catatgcata attattctac ctccaataaa
     7381 ttagttatca attcatcatc tgttagtagc aaagccaaga acttatcatg gatgtttaaa
     7441 aacaaattat cctcattcga tgtaagctct cgttgtctcg ctcgatcata taggttaagc
     7501 agacaattaa attcatccat tagctcacct gtatcatcat agttgccact atctaccaat
     7561 gatcccacta ctagcgattg acaccacgct actgactcct gcagccaatc aggttcttct
     7621 tccattatct ttaatacttc tggatcaaat gtggaataat cttctgttac caatcccaca
     7681 ctaaatgcca gcgtgttcat tagaaaccga tccatttcat ctgtatattg aaagttcggt
     7741 gctttgtttg ttgaaataat attgagcatt tactttcctc cttgattcgt aacacgtggt
     7801 aacacctacg taacaggtac ccgttaccac tcaatcccaa cggggacaag ggttctagcg
     7861 ttttggtaac aggtaacggg taaaatccag taacattatt ccccagcata ttagctaact
     7921 atttcaaaag ttttaaggaa ataaggtgtt accccgttac caaattgctg tatcccttgg
     7981 ggcagtaagg gtaaactagt aacgggctag gtgttactga cccgttacta cccgttactt
     8041 tttgtacttt ccagctcgat ttttattttc ataggcattt gacttattgt aaaatcctcg
     8101 tcgtttattt ctttttggca gttgcttccg ttcccaacca ggaatactgc taataatcaa
     8161 ggcaatcttt ttagtttctg catctgatcg accattcaat aaatcctttg cttgtttatc
     8221 aaaaacagct tccaaaatat cagcggtgag tacgctatca actaaataaa actcttgttg
     8281 catagtagct ccattataga aatgctgtaa atttttctca ttacatttac tgtcaggata
     8341 gttctgataa taagaacgtc tcgcccacgc tggagcttct tcccatttat acgggatttc
     8401 catttccaaa tatttagtga ttgcttcttt aattggattc tccatcttgg catcctcttg
     8461 atagtctttt gcaatttcta agacttcttt atcttcatcg ggactaaaat agattcgttg
     8521 tcctttatca aaaagcacct ttgcttcagc taacacttga agaaaatatt catcctttgt
     8581 tttaaacgga ttcatctttg gcttattctt acaaggcaaa ggataaaaac ggcggtttcc
     8641 tgtatcatct ttcaaatact gcccatcatt cgacgttcca ataaatacac attgccttgc
     8701 ccaagctatg acattccgac cataaggagg tctaatttta tcgatcttgg cactgatgaa
     8761 gttttttatc tgttcaattt tagttttgtt cattgaagat aattctccaa gttcaataat
     8821 tacattgcct tgaagctgca tataatcatc tttcttttca cctagcgaat ctaatgaatc
     8881 ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta ctcttaccaa tcccttgcgg
     8941 tccatccaaa actggaacga tctcaaactt tattcctggt ttatagatac gtgcgactga
     9001 tccgactatc caacgttttg ttacttctcg tgtgtaaagg ttgtcgtcta cccctaaaag
     9061 gtcaataaac agtgtttcca gtctaggctg ttgatcccat ttaacttttt cgatacgttc
     9121 ttttactggg ttgaaaacat tctttctagc cacatttact actgctttgt gtattttgtt
     9181 gtcatcaaaa actacattgt agttttcttc aatatatgcc gttaaggcat cctcaaaact
     9241 gtcttcccat tctccagcgg tacgattgat ccaaggagaa tcttttaata ggtgaatgta
     9301 tccgctaaat tcgttataag caattgttcc agctaaattg ctatcattgg ttaaaatcaa
     9361 ttctgcattc acaacactgt tacttttaat ctgatcgtct ttattgcatc ttaatcgtga
     9421 catccaggtt ttcaattcaa tgatattgtc ttcacgctcg ctggcttcgg gaaagtctaa
     9481 aatactatct aatttatgct tatgagccat gagctttccc cctcaaatct tttttaatca
     9541 tcgatcgaat aatttgattc acttctttat cttttagcgg tggttcattg tacgcatttg
     9601 ctgtataaat caattctgct aaagtttgat tatctacacc gtgccatagt aaccaaccgc
     9661 ataacttagt taaatgatcg tttctactac cttcgccagc gccttgaaca atcatatcta
     9721 atttttttgc tgtccctgtt tttgctacgg gtcggtattg agtctttatt tcttgcggtt
     9781 tttttagcag taggttcact agccaatcgg gagctggtgc aatattattt agagatacgt
     9841 tttcaatagg ttcatatgct ctcccaacac ttcttgaaat aatcatagtt ggcgctattg
     9901 gaactccttt agtgataata tcaatcccgc ttaaactttt acctttatca tcacgcccga
     9961 agatatcagc aaatgcacct gtttcttgtt ggataattac accatcagga attttaaaga
    10021 agaaatggat tcctttacct ggtgtgattt ccgtataagt agaaggaaat ggatcataca
    10081 ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt ttcagaatgt tgatctaggt
    10141 caactaccac catttgatga tcttcaagca ttaacccaat atttctagtt gcatcttccg
    10201 tccaccattc attaatttga ttagaatcgt tagttgcatc attccaacca ctagagcctt
    10261 ttattggaat tttagttcca ggagataagg gataaattga ccatcccgac ttttgtaatt
    10321 ccgttgctgc ttgataatta tttgccaata aacctctcct cctttttcaa aatactggta
    10381 taaatcattc agacacctcc atgagcttca actgctcaag ttcgtatttc aacccatccc
    10441 gaacaaattc agcacctttt aattgattct gatactttac ccatgtatta aatgttttca
    10501 cccttgcttc ttgcatatca gtaatgttta accatgcggt taaaatctga ttttttagct
    10561 cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt tttgagtaca tacaaaactg
    10621 ttagtttttc attgaatgaa tttccaaagt aggttttccc gatattattt tctagttcag
    10681 cgatcccttg attaatttcc tctaattcaa ggctatcgag ttggtttaat agttttgttt
    10741 tgtacatcta cttatgccct ccagttctta attgatagtc gtactcgtca taagcagtga
    10801 agagatataa cgctaatcct gcaaagatag ctatttttgc aattaatggt aataagccga
    10861 aaattccgcc gattaaaatg cttcctataa ttaagtttcg tttcataatg gttccctccg
    10921 attttttaat atggtataat cggaacaaag aatatcgggt tttatcccaa ttaagttgcc
    10981 tttatcctgt ccgccaagac tgttatggat aaggcttttt ctttgttctc gatcatgtta
    11041 atgctcctaa gttgtctgca attcagcaga acgattataa attttctcac tcactgaaaa
    11101 ctgcaaccca tatttttctt taacagtcat taattctact gtctcatcta aaataggttc
    11161 acgatcaacc aacatttcag gagtcatatc tttctttttt accattttgg aatgaccata
    11221 aatagttgat acagctttat tggctatcac attcgcttta atgtagtcta ctggttttgg
    11281 ttcagtaata gcgtggctta atttagtcat tgcttctttt tggtgttctt tgtctagcat
    11341 gcggaatact tgaaagcctt caagtcctgt ggactggcgt agttctttga tgatgtcgaa
    11401 aatccagtct tgaaatgcct cggcttcttt tttacggctt ttgaatgcca tgcggtaaat
    11461 attttttttc gttgatgatg tttacatctt gtgttctgcc caatgaatcg atgaccttac
    11521 tagtagtaac cccatcttta ggcaaagagt gaatggctct agtaacctgt ttaagaccta
    11581 acgcttccgc aacatctttc gccactgccc accattcatc attaatatcg acaaatctta
    11641 taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc tccagttcaa gccctagcaa
    11701 gctcgctaac aactcaactt gctgatacaa aatttcttga tactcttcaa tagaaagtcg
    11761 ttctttaaac tctgcttgta ctaaatcgtt caagccacgc aatactccgc ttaccgttgg
    11821 ttcttgtggt agcaattttt gatagtccat ttgttcgtac aaacattcaa acgtttcttc
    11881 tagcgctttg ccttccaagt ttgctaattc tgccgctgtg tcttctgtaa gaattgttga
    11941 taatactttt tcgattgctt tattttgtgt cgattccatt attatttcct cctattaatc
    12001 cctttgcccc cacggcttgc gtttattttt ggtattcttc cataaagcga tcacattcat
    12061 cttgtgaaat tcttacgact ccatcaacta taatcaactt taaaccgtta ggaacatatt
    12121 tctgcatcat agtattgtat gaaacgttca tatacttagc agcttgtgcc ttgttcatat
    12181 attttggaaa ctggtacttt ttttctaata atgaaagtaa cgttccttga gtttgctcta
    12241 cttgttgaac aactcgatcg tcaataattt taaaagctgg atcatcaagc gtgagtttgt
    12301 caatttttaa ttccatattt atcccccctc gttagcatac atttctatac aaagatttta
    12361 aaaaaatgca agaggctctt gaatacattt ctatacctct tataaactca atataccatc
    12421 ataccttttc atttgcaaga attttaacat acttttttga acttttatgt ttcacacata
    12481 tttaaatcgt gatatagtag agaaaatata tgaatggtgg tgaaaatgtg gctagaaaaa
    12541 aataccccga agcagtggag acaggacaaa gaattagact ggctagaatt gaaaagggac
    12601 tctcaatgga acaacttgga ggtaaattat ccccaccggc cagtaaaggt gccgttagta
    12661 attgggaaaa tggctataat cttcccaata acaccaggct taaacaactt tcaaaggtat
    12721 tagatgtatc cactacctat ctattaaacg gatcttatac attaagagat atgcatatga
    12781 tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa aaaaactatt gaaaaaaata
    12841 actctataat tgattctaat ctttctaatg ctatcaataa catcgaagaa gataacctga
    12901 ctgtctcaca gaagatttat ttaacaaacg caataaaata tcttaaaaca ggatctcgaa
    12961 ttgaatttcc tgacgggagt accccaattg cttatctcgc agctcttcta aatacgcttt
    13021 tacgatatga ctcattaatt aatgattcag aaaaaaatac agatgagaaa aaagagtatc
    13081 aagaatttta tgaaaatgat cttctaaaag agttcacagc tcttgttact gagctaaata
    13141 aacactattc aaaataataa taaactgcct cctccaaccg cccccacggc ttgctacact
    13201 atggagggaa aactattatg gcaacattca agcaatatga aactaaaaaa ggtaaatttt
    13261 ggctctatga agcctatctt ggaatgaaca aaatgactgg caaacctgat aaggttcgta
    13321 gacgaggtta caaaacaaaa aaagaagccc agcttgcctt gtcacgcttg caagtggact
    13381 acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt taaagaagtt tatgacttgt
    13441 ggctagaaac atataaaacc actgtccgag aagccacctt catgaaaact gaaatcaagt
    13501 ttagaaagtg gattcttcca aaatatggaa atcttcgtgt gaatgaagtc acggtaaaaa
    13561 aagcccaaca aattgtaaat agctgggctg aaactactga ccaatacaag gtacttcatt
    13621 caacttctaa gcgtattttt agctacgcta tcaatcttgg gattattgag actaatccac
    13681 tagacaacgt attaatgcct aaaagaacca ccactaagca taaagaaaag gtcaaagttt
    13741 acagtaaaga acaactgatc accttgtttg attacctaaa cagcaaacct gaaacttatc
    13801 gaaacgactt tgataaaacg ttactacgct ttctctttta tagcggggtt aggatcagtg
    13861 aagcccttgc attgaattgg tcagacattg attttcaaga aaatacggtc accatcaata
    13921 aaacactaag ccaatccaaa aatggctata aaatcagtga tcctaaaact gattgtagtg
    13981 ctggggttct tccactagat gaaagaacaa tttctatatt aaaaaaatgg cagattaatc
    14041 agcggaaata tatgctgaca cttggtgtaa ctgatccgac tatgattttt tgtggtatct
    14101 ataaagaact aatcacacac cacgctattt atgcaagatt gattactatt tgtgaaaaag
    14161 ctatggttcc atttctcggt gtgcatgtag caagacacac tcatgcttca atgctcttgg
    14221 acgctggagc tactatggta gaagttcaaa ctagattacg gcatacaaaa attagtcaga
    14281 cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga aaagactgtt gaaaaattag
    14341 tacaacactt aaatattgac tagatggcat acaatatggc atacagtaaa aaataaaaaa
    14401 aggagaaacc ctatcatatc aaggtttctc ctagctttat agtctattct tcttcttccg
    14461 ccatgaatgt attgaatact tcttcaatca tgtcccattc tgcgtctgtt tcgatttgtt
    14521 ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc ataagcttgt aattctacat
    14581 cttcatcttc tgaaacacca gcaggatata aaagaacata gttacgtcca aattcttctt
    14641 gtccgtcaac tgttagtagg atttcatata atgtttcatt tccttggtca tccaccaatg
    14701 tgatgtgttc gtgtccttca tggtcgtggg aatggttatg ttctgtcata agattacctc
    14761 ttttctttta ttattaaaat aattgcttac ctgagccgtc taaataattt tgtaagatca
    14821 tcacagctgc taatttatcg atgacttttt ttcgtttggc tcttgaagta tttgcctgct
    14881 ctactagcat acgttctgcc tgaacagtgg ttaatcgttc atcttgataa tcaactggca
    14941 aggagaatag ttctttgatc ttttctccgt atgacatgga agcttctgcc cttggtccga
    15001 tggaattgtt catattttta ggtaatccaa ctacaaaccg gtctacttca tattgtgcta
    15061 ctaattcctt caaccgatca aagccaaact cttcttctgc ttcattgatc cgaatgatct
    15121 ctactccttg agctgtccag ccaaaggggt cactgatagc aacacctact gtcttagaac
    15181 cgacatccaa gcccattact ctcataaatt cactccatga tttgagaggt agtccttgac
    15241 aattacttcc atgatctcat cgcgttcgtg acggcgaatc aaatttcgag catcacgata
    15301 acgaggaatg taggacgggt ctccagaaag caagtagccg acaatctgat tgattggatt
    15361 gtagcctttt tcttccaaag cccgataaac aatcgctaat gtttcgctca cttctttttt
    15421 gcgactatcg tcaaaaccaa aacgtacggt ttcatctgta aaacccatga ttctacacct
    15481 ctttcccgca aggtaattcc agcttacctt ctacaacctt attttactcg aagaaaggga
    15541 aaactacaaa caaatattcc aaacttccga ataaattaag aaacactatt tgtatgtttt
    15601 tttccttttt tctgtagctt tatcaaaagt actcttttca taaagtatac gctatatttc
    15661 tttgaatgta tgtttttaca caattttttc ataatattca taaaaaattc gaatagctgc
    15721 tactaatttt ttgtttttgt tgcttgtcct gtctcataat tgatcgtgta gccatcttga
    15781 acattgtaga taaagacatt gaattcaatt tttttgtctt caatgctttc tgcttccaat
    15841 tgcactcccc tggctaccag ttcattcccc tcaaaatgag gagtgacacg gtagcgtaca
    15901 tgatgatttg tttctttgat atatgccgca atatcatttt cgtgagcaag catgtatgga
    15961 gcattcaacg aacgtgtccc tgtcatcaag tttttaatgt tgttattttg accagtcagc
    16021 tggaaaccaa ttaaatggct gcgattatat aaccattgcc cgtcacttaa tttcttttgt
    16081 ttccaacctg tcgggtcaat atacaaaggc tctctctttt cagtagggaa caaatctttt
    16141 ccaagcatag cattggcttg tcctactctg ttcaatttat caatatttga atagctttcc
    16201 catgttcctt tggaaaggct aagatcctct tttgtaaatt ccggctgatt cccattgatt
    16261 tctatttctt gatgttttcc atcatattct ggcacatccg ctacttgaga agcttgtggt
    16321 gtggaatcaa aaatagttgt aatctgctga aagatatctg aatctttttg tgtggtggaa
    16381 acaccgaatg cccctgcaat cacagcagcg acgatcccca cgatcgcctt ggttgttttt
    16441 ttgctcattt ctttactcct ttactaacag ctccctaaat cttaacatat gttcccctat
    16501 aaataaatac tagaaagaaa tagaaacagc aaaaaataaa acctgtttta agatggctta
    16561 aaaatacaaa aaagagacgg attcctcgtc tctaatcagt caccactcta tctgtaaggt
    16621 atttattatt attcaaaaat tttcttcata ctcaagacaa tacactgatt tcacaaccgc
    16681 ttggtaaacg gtgcgcaaaa gcttgcatct gcggtaataa gtccatccaa gttaaaaaca
    16741 taagaagttg tttttgcttg gctgttctta ttacttgttg cagagcagct ttttcacaac
    16801 ctattttaag cgggtgacgg gaatcgaacc cgcgacatca gcttgggaag ctgggatttt
    16861 accactaaac tacacccgca atacaaaaac agtatacgcc tcttttattc cttcctcaag
    16921 tttttttatt ctttacgctt ctggtatgtt ttgtagtaaa ataaaactaa tcaatcaatt
    16981 attggagaag atgctatgac gggaaaagaa ttagatctgt tgctggacac ttgtttactc
    17041 gctgggaaaa tcatgatgga aagcaatgcg gagatgtatc gggtagaaga tacgatgagt
    17101 cgaattgctt tagcttctgg aaatttccgt ttggtcagct atgtcactca aaccggtctc
    17161 tttattggtc ttgatcgtac ctcgaccatc cgaatggaac aaattaccaa ccgttcgatt
    17221 aatttagaaa aagtagtaaa agtaaatgat ctttcacgta aatatgttgc ttcagagcta
    17281 acattagaag aactttactg cgaactgcaa gcaatcgacc gagaccgttc tttctttcct
    17341 gtctggttgc aaatcgttag cgcggcagtg atcagtggaa caattatggt gttatttggc
    17401 ggaaagatga ccgatttacc tgtgacttta gtcattggag gattgggata cgcagcttat
    17461 ctatatagtt tgaaattttt tagaatcaaa tttttatcgg aatttttatc ttctttattg
    17521 attggcatag ccgctatcta cagcgtacgt tttggcttag gaagcaatca ggatctaatc
    17581 attatcggat gtgtgatgcc tttagttcct ggagttcaaa ttacgaacgc tatccgtgat
    17641 ttgctagctg gacattatgt ctcaggagtt tctcgcggaa cagaagcgat gatgactgct
    17701 acgatgatcg gttttgcgat tgcttttatt ttccagcttt tttattagga aaggaacaca
    17761 agtatgggga cgatatttat tcagttttct tttagttttt tagcgactgc ggcatttgcc
    17821 gttattacaa atgtacctag gcgttcattg atttgttgtg gtctttctgg tgcttttggc
    17881 tggatgatct actggatctg tgttcaaata ggcggatctg ctgcttttgg ttcattgctt
    17941 ggttctttag gagtagcatt catcagcgat cttttctcta aacgtttgaa aatgcccgta
    18001 acgattttca atattcccgg gatggttcct ctagtccctg gtggtttagc ttatcaagcg
    18061 gttcgaaatc tcgttactga atcctatcga gaagcggctc attatgcggt ccaagcaatt
    18121 atgattgctg gagcaatcgc tttaggtctt gtattatcag aagtctttaa ccataacatc
    18181 cgtaatttca aagtaaagcg tgacgcaatc atgatgaaac gaaaaaaacg gctttttaaa
    18241 taaacgaagt gtaacaaaga aaaggttgtg aaagagacgt tcagccttga gcgatgacaa
    18301 aatatctcac aaacattgcg acaagtaacc gcaggagcaa taagaagtaa ttatgagtat
    18361 taaaaaaagt tttgtaaaaa tagcttctca catttttaca taaaattgac ttaatactga
    18421 gaaactgact tttgaacacc ctctatctgt aaaaaagggg ctgcgacatg atttttgtca
    18481 cggcccctct tctttattca aattttttcc aaaacgttcg ttgataataa acgatccata
    18541 gagcggaaag tattagaact aaaccgatca caggaatatt cactaaccaa aatggataaa
    18601 acaaagccaa cattccgtat aagacgataa gaataatact gatgatcaat gaacccatca
    18661 tagttgcgac taaacccaaa cttcctgaac ctctgttaaa taattggtta atattggtcc
    18721 agttcagcag cagcaaccga taatctctag cgaaataacg tagtgacaat aagtagcagc
    18781 cgaataaaga accaaatagc aagctaggga taaaaaacaa aggcagctga aaagacagtc
    18841 cgcttaataa tgcgatccca ccagttaaaa aactctgcaa tatccatccg aatctgaact
    18901 tttcttttaa atattggttc atagagattg gcaaagaacg gataaacaga aaattttctt
    18961 gatccaaaga aataagattg ctgataaaag aagtctgatt aactgttaag aaagccaatg
    19021 cgatccctac caggaaaaca acaccgatta acttattatc taaatcagtt aattttattt
    19081 cgcctcctag ggcaaaagca agaataaaaa tgattggagt taacaaggaa gaagaaagaa
    19141 cttgcataat caaattaggt tcttttacta attgagcatt gtaactaaat aaaagctgat
    19201 gcacattttg gttcttttta tgggttcttt ttattttgaa ctcactgttt gaatcagtca
    19261 gttgctctgt aagctttggt actatcagct gttttacagc aaggaaaaat cccagagccg
    19321 tgatagctag tattccccaa cccaataaac cttgttgaga aagtggctga ctggcagcat
    19381 catagaatgg caataatatc gaaatcggat gccgatctgt taactcattt tcaatagtcc
    19441 ccgtttgatg attcatccac atgattccag caaatgcgat cagcatcgag ctgccaagca
    19501 agatactaga aactgttttt ctatgttttt taaacactgc tgttcgagcc aaaccaaaaa
    19561 caatcaaact agccagacaa aaaagaataa tcaaaaatag agaaaacaaa aggattgaga
    19621 taatgatagt aagcaaaatc cctagatcag ctcgccatcc ggttaaaata aacatgacta
    19681 gaagcggaaa aacaaaagga atgatggtca aagcaactac caaaatcttt gctgaaaaaa
    19741 cctgttcttg tccaaaaggc agaggcaaaa atacttgcag gtcatttcct tcaaaaaaga
    19801 tattataaat gactgatata ctttgagtta aacctaacat accaaaaagt gccacgtaat
    19861 aagtaaagaa acctggcaat ttaacaaagt tcatagcaaa catagcaaat ccgtaaatca
    19921 gtaaaaaaat cagccctgaa aaaagatttt gagacaaaag atagatagtt aatcgctttc
    19981 ccttttttcc tttctctctt gctttattcg tgagctgtgg attaacatat aaaagattaa
    20041 ctcgaattaa ttctaataat tgtcgcttat acatacggtc catctcctgt cgcttgcctt
    20101 ccagccattt tcaaataaat cttttctagc gtttcatcag gtgagccatt caataattca
    20161 ctaactgccc cctgataaat caattcacct ttcttcaaaa tagccagttt atcgcataac
    20221 tgttgtgcgg tatccaatac atgtgtagaa aaaatgactg tttttccttt tgcggcatga
    20281 gccttcatca tttctttcaa atcaaatgct gcttgaggat caagtccttg caaaggttca
    20341 tccaataccc aaatatctgg atctggcagc aacgtgccga tcaaaatcgt tttttgacgc
    20401 attccatgtg aaaaacttgc gattgtttca ttttgatgac tatacatatc aaaaagcgct
    20461 gtcaattctg ctaaacgctt ttcttttttt tgtgtgtcta attcataggc agctgaaatc
    20521 aagtcccaat attctccagc cgtcaattgt aaaaaaatat ctggggaatc aggcacataa
    20581 ccgatttttt gtttgatact caatcgattt tccgtcaatt tcattccatc tactaaaatc
    20641 gttcccgatg atggttcaat aatactaact aaacttttaa tcgtagttga ttttccggca
    20701 ccattatgtc ctaaaaatcc aaaaatttcc ccttgtctta tcgagagaga caagtctttt
    20761 aatgcttgtt ttttcccgta acttttactt acattgatga attcaatcat ttctgtactc
    20821 cttttgtttg ttattacttt ttattgtacc cttcgttatt ggtggagtaa atgtcaaaaa
    20881 gagtgcataa caaaaaagga tagaaacgac taggctgcta cttgggttgt tctcttctac
    20941 aggaaaacac tcttctaaca ggtaagatat gagtctttgt tttctttaaa atgattcatt
    21001 ttctatcttt tcttgcataa aattctttag tttatcaata cttctctttc ccatctctag
    21061 ttgttctcca tttgcaaatg ttactatttc gtttactctg ctaatagatg taatgttttc
    21121 agtattaatg atataagctt ttaaatcttt acataaatac tcatattcga gtgatttttt
    21181 atatttttta attgtgccgt ttacgagaat ctcttcatct ttcaatacta ggcctatttt
    21241 atttttataa cctggcatgc tcattagata aaggatttca tcgtaatgga tatagtgatc
    21301 gctattacca taacggacag ttgtccacct ttttttcgta gcatgttgaa caggatagtt
    21361 ttctttttct ttcaataagt aactttggat gcttttttta tttttttcat aagtgaattc
    21421 ttttatcaaa taaccactag gcgtaatctt tcgattaata acagttattg ctttattcgg
    21481 atgagacgtt agataaacaa ttttacactt ttcattcttt tctctaagca tttcgcctag
    21541 ttcgataccg ctataatagg cgttcaaatc gatgtctagg aaaaaaatat ctgaggaata
    21601 gatttcatta tttttaatga attcaacaaa ttgtataacg ttcaatccct gaacgatctc
    21661 aaatttccaa ggacttaata aaattctgga ttcttccaaa atttctacta gcattttttc
    21721 aaaaaccaaa ctatcatcca aaataaatat acgcgttacc ataaaaagta cctccactcg
    21781 atattttact atcaaaatca tttcatacaa cttttcaaca tacatgttag gaataaattg
    21841 taattctctt cacaatcttt tccaacacct ttgaaaccga tatcaaatac tttcaggatg
    21901 ctgcttctaa aataaatatc aaacaaatga atacatcatg caaaaaaagc cagacaaccg
    21961 acgtccttgc gtgtcagctg tctgagctat gtttatttat tttgttcttt taaaaagaac
    22021 gatacacata ctgatttttc ggagcagaga ctttctcagc gtttgtaaca actacagatg
    22081 ggatttcacg cttgaatggt tgataatagt ctgattggat ggttccttct attttatacc
    22141 attcatcatt tttaaccgtt actccttccg gcatatgcgt caacaaacca aagactcctg
    22201 aatctgccac acaatggata atgccgaatc ggaacaagaa gatatctgta ttgctatcgt
    22261 tcggtgtttg gtaaacaaat ccttcatagc tgattttttt gccaatgaat tcgctagggt
    22321 aattataaat tagctccata acttctaaat agttttcatc tgtgatcttg atagtttgct
    22381 tatccccata cttttctttc aactcattca tttggtctaa gtagtctgat ttgttgaaat
    22441 aaatactggt atccggcttt aagtattgtg tctgcatctc cggatcccct actgattctt
    22501 tacttactgg gaaattaaat cctttggctt ctacgatcgt agtgtcgaga ctaactgttg
    22561 gaaacaacgt ccccacgatt actggcaaag ctagcagtac ataagcgatt ccacgttgat
    22621 aaggttttga caacccatgg tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt
    22681 ttttgaccca caaatacagc tgaactaaag ccaaaagagc agataaaatc attgaaagaa
    22741 tagctaagta gcgataatgg acattgatat attgatcgag tttgccagaa acttgcaagt
    22801 acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt tccctcctaa
    22861 ataaataatg tgaatccaat aacgctaagg gcaatcagtc cgatcagcga taaaataaag
    22921 cgtcctgtaa aatatcgttt catcatcagt agatttttga tatcgaccat tgggccaaag
    22981 accaaaaagg cagctacagg tgctgtgccg aacagactta acaaagaaga tcctataaac
    23041 gcatctgctt ctgagcaaag agacatcgta aaggccaata acagcatgat gataatggct
    23101 aaaatctttg ttgagccgag cgttagcata attctagttg gtaaataagt ctgcatcgca
    23161 gctgcgatta atccgccaat gatcaaataa cgacctgtat caaaaaattc gtcgattgaa
    23221 tgtgtcaaca catgcccagt ttgatggatc agctgatttt ttggttcatt cttctcactt
    23281 tgttcttctt catggacatg atgatggtga ttttctgcta ctttagacgt gattttttct
    23341 ttcaatacag cgtctttttg aaaataggca agccagacac caatcacaag ggcaacaaaa
    23401 aagctgccta tcacccgcca gcttgccatc tgccaagaat tgccaaatgc gataaaagtg
    23461 gaaaaaatca cgattggatt gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga
    23521 actggtacgc cttttttaac aaattgatga acgatcggta cgatgccgca ttcacatgaa
    23581 ggaaagaaaa atcctaagat cgaaccagta aaaatagaga agatacgatt ttcaggaagc
    23641 catctcttca cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat aagacagcct
    23701 aataatacga aaggtaatgc ttcaataacg atcgataaga aaatcgtact catctgcaaa
    23761 accgaatttg gaataaaaga aaacatgagt ttcctccttt ttatttctag ttcattcgct
    23821 ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc
    23881 ttatttttta ccaaagcact tcaattgact ttttctgtta ttcccattaa attaaggtct
    23941 catacatatc acttctatca taaaagggag aataaaaatg aaacaaacca ctaacttgac
    24001 tgaagttcaa gacatactgc aacagtcatc cgttgccgtc atttatcttt cgatgcctaa
    24061 ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa cttctcacac attatgatat
    24121 tccggcactt catttggatg catttgaaac accagaagta gctagtaaat ttgaagtgct
    24181 gactgcgcca gttgtcctga tttttcatca aggaaaagaa gtctttcggc aagcacggtt
    24241 catcgacttt aaaaaaatcc gttatctact agatgaactg acagcagaag atgattccct
    24301 ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa aagcgattga
    24361 acaagtgaaa gttttgcctt catttgttca atcgcttttt atttttattc tgctgtattt
    24421 tcggaatgcg catttgcgtc agaatgcaag aaatagatca aggtttgcaa ttcattcgtc
    24481 aaatcaacat tttggatcaa tacatctgac ggagcattca atcgagcagc ggtaaagttc
    24541 aagattcctt tgattcctgc ttccgttaac tgattgacta cttcttgtgc tctttcagcg
    24601 ggtatcgtta aaattgctac ttcgatttgt tggatcttga tttgttctga catatcttcc
    24661 attggataaa cggggatacc gtctacgatg cgcccaacga tttcctcatt cacatcaaaa
    24721 gcacaactga tacgtatgct attactttga tggaatttgt attttagtaa agcacttccc
    24781 aagttcccta caccgatcag cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca
    24841 aaaaagtgca taagatcttc tacatcataa ccatacccac gttttcctaa ttcgccaaaa
    24901 taagaaaaat ctcgacggat cgttgcacta tctacttgaa cggcttcact taattcagtt
    24961 gaagacactt ttttctttcc agcattattt aaaatcctta aatagcgata atacaaaggc
    25021 agtcttttag ctgtcgcctt tggaataata tgatctttca cgaaatgacc tccacgtatt
    25081 gtctaattac atctttgatt tctccatcat agcagtattt ataggaaaaa atcaattcca
    25141 ttgctcagat attgtgaaat tttattcttt attcatttat ttatcatttc tgcaaatctc
    25201 gcagtataat ggaatttatg gtagactact aattgaaaaa tagtttttta tttgaggaga
    25261 attcacaaag tagaaaagag gattccatga tattattaca agccaatcag attgctcgcc
    25321 attttggtgc agaagtctta tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg
    25381 tcgctttagt tggcagaaat ggcgctggaa aatcgacttt attaaagatc attgctggaa
    25441 ttgaagcacc tgatgaagga aagatcgcta aaaataaaac agcgactctc ggatacctcg
    25501 ctcaggacac aggacttgat tccaatgaaa cagtttggaa cgagatgctg aaagcttttg
    25561 aacaagttag acaaatggaa gagcggatgc gcgagctaga gattttcatt agcgaaggta
    25621 cgccagacac acctgcttat agttcttatt taaaagaata cgaccggctg caacacgatt
    25681 tttcagatcg aaacggctat ggttatgaaa atgaaattcg ttccgtattg catgggttta
    25741 aattcgattc ttcattttat gatcagaaaa tcgatacttt atcaggcgga cagcgcacac
    25801 gtttagcttt agctcgcatg cttttgcaac accctgacat tttgattcta gatgaaccaa
    25861 cgaaccattt agatatcgag acactttcct ggcttgagaa ttatctgcaa agctatactg
    25921 gtgcattatt gattgtttcc catgaccgtt attttttaga taaagtagtc aatgaagtct
    25981 atgaattaag cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa tatctagatt
    26041 tgaaagcaga acagttagca agtgaatgga aggcttatga aaagcagcaa acagaaatcc
    26101 ataaattaga agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc
    26161 aaagccgcag aaagacatta gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa
    26221 aacaagcaaa ctttctattc agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg
    26281 aagatgccgc tatcggatat gataatgtca ccttatctga accaatcaat ctagatatcc
    26341 gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat cgggaaatcg acgttgctga
    26401 aatcaatcat tcaccagatc ccttttatcc gtggaaaaga aacgctaggg acaaatgtca
    26461 ccatcggcta ttacgatcaa ggtcaagctg atctgcatac aaacaagacg atcctagcag
    26521 aattgtggga tgagcatcct accactccag aaaaagagat ccgaagtgct ttaggtgctt
    26581 tcttattttc tggcgaagat gtagaaaaac caatccctct gctaagtggc ggtgaaaaag
    26641 cacgggttgc tttagccaag ttagcgatga acaaagaaaa ttttttgatt ttggatgaac
    26701 caacaaacca tttagatatt gacaacaaag aagtattaga aaatgcattg atcgattatg
    26761 acggcacact gctttttgtt tcccatgacc gatatttcat caatcggatc gcgacaaaag
    26821 tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg agactatgat tattatttag
    26881 agaaaaagca ggaagaagaa gaaatcgccg aattgttggc acaagatgag ccagaatctg
    26941 ctcctgctcc cacgaacaaa tctgattatt accaaagcaa agaacagcaa aagcttatcc
    27001 gtagccttca acgaaaaatt actcaggttg aagaagaaat ggcgcggatc gatgaatgga
    27061 tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac gtaaaactca
    27121 atgagctgaa ccaagattta gaagctgcta gacaagatca agaaacaaaa ttgacggaat
    27181 gggaagaact aagcttggaa ttagaagaat atgaaaatta acgaaaaaac caaaacagaa
    27241 accattcatg ctgttttggt ttttcttttt ttattttatc aaacaaaaat tccatattta
    27301 cctttttcac caataagaag accaattact agtcattctc actaaagctt ctaaataata
    27361 gtaatcaccc caaatacaag gtgtatttac cccattattt ttgggcttat tataaacaga
    27421 ctgtgttaaa aagccatctt cttcttccgt tgtagcatat ctttctttca attgtcgcat
    27481 aattttccaa acagcagact ggtataagtt cttttctgtt ttgtctaata gacctgattc
    27541 tattaattct aataatccac aaaccatgat caccatacta gatgtatctt tttcttctct
    27601 gctagaatca tcaaagatca aatcccaata acaaatggta tcttctggca accgattcat
    27661 aaaataattg gctaatattt tgcttaaatc aaaatatttt tgttctttca aataacgata
    27721 acttaatgca aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg aattatcact
    27781 ataaccttga tgcgtacttc catatcttgg ttttccatct ttgacatcca tataaaatgt
    27841 atgataagtt gaaccatctt ctcttactaa ataatctgct gcattttgaa tatgagcctg
    27901 agcagctttt ttatattttt gatttccagt ttcttcactc gcccaataca ataatggaag
    27961 attcatgcaa caatccatga tcattctacc ttgttgtgaa ggatcactag gatctcccca
    28021 agcttgtata actcctgcct tctcccaaaa acgctgaagt aatgtttctg cagcatcaat
    28081 tgaagcttcc ttcatttttc gatttcccgt tattttataa tctgctacgg ttgataaaga
    28141 atacaaaaaa ccaagatcgt gatgattagt gataatttga cagtcaatcc tgtggataaa
    28201 atctatatct gctttttcag ctgtttgttg aaataaagga tcacccgtct tctcaaataa
    28261 caaccaatta attcctgtcc aaaatccttc tgtccaacca atattgtctc ttaatggata
    28321 acgtcctttt ataggacact cgcttgggaa ttgatactga ctgttactga tacgtttgat
    28381 taacctctcc gtgtgtctgc gacaacgctc gatctcatta ttccattctt tttccattaa
    28441 ttctttataa cattcattta tccatttttc tggatgtatt atctcttcaa acatcacttt
    28501 atcaagcatt tccccaccag cctttagcat aattttagtc tttttttatt gtttttattt
    28561 tggatagtta gcccggttgt cccatcaaaa tcaattttgg gacgattgtt ccatacttct
    28621 aaactataat cccgatcttg atgagcaacc actcctgttc cgagccaatg ggttcctttt
    28681 ccaaatgtac cctctaatcc aggaataaaa gtgttttccc atgtcatcaa gtttgtattg
    28741 ggcaaaccca taatagctgt ggattttcgt ttaatagtat cgtcacttaa acagataata
    28801 cctgaaaagc cagcttcact caccctatag ttttccttgt cttcttctat gattattcct
    28861 tctttctcag gagaacattc aatagcaaac cctgtctcat atagaacata ttctgtatct
    28921 aattctactt tgtgtacgcg aatctgccag ccttcaaaag gaataagcca agttctgatt
    28981 tttgcctgta aagtaggttc ccatgtagag gcaagtacct ctttcttaca tacaatgtct
    29041 cgtacattat ttcgaacgat gaactgtcca taggttttga tgctctcatt cttaaaactg
    29101 gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg aatccattgc catttctgct
    29161 ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc aaattttaaa
    29221 tatttttcat tggtcaatgt atggaaattt ggccctaatt gtcccgcatt caaaaaaaat
    29281 gtatgtccat tatcatgttg tatgagcaaa ttggcttctg aaagaagttt agttccttca
    29341 cgaataggca taggcatttc tttagccgtc caatactcat ggtcttcagg tagcgccaac
    29401 aataaaaaaa tcttattcag ccataatgga gataacgtac tattataggg ttcagtcaat
    29461 gtcaattgag ggtaggtata accaatattc aggatacctt tttcatcaaa tatttctttt
    29521 tgcatccacc atcttatatt ccgattgact aaccccttga tttcacctaa tttaaaaggc
    29581 aaaagatcgg cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg
    29641 cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa aatgaatata gtctctagaa
    29701 aaatcaatag ctcggttctt atatatctgg ctaatgggct tttctggttc tagtacgcta
    29761 taaatcaaac cataataatg aaatgcaaaa ggattataat aatcttggcg acctcttgaa
    29821 ctgtctcgat accagccatt ttgaagataa caattttcta tattatcaaa tgcttctttc
    29881 agtgctgatt ctttaactgg ataaccaaat ttttggataa ttgttccgac aattatctta
    29941 aaaaattgcc aattaccatc tgcacattca tattcaaaaa tttgcgagaa ccaatgaata
    30001 atctggcttt tttctcctgt atctaatttt ttccataata attgttcatg atgatataac
    30061 gctaataaca ttgcgggcat ttctaccata cgctggtctc tttcatgtat ttctccccaa
    30121 taatcgggat gggaaggatc tgttccagct tttattcctg atagaattaa tttcaaccat
    30181 cgttcatcta attggtcaat tgcgggcccc gctccccata aaacccgaga gaatccttcc
    30241 attcctgcaa ttttatttcc atatcctacc ccttgatgta tataatcaat ttgtgcgcca
    30301 cttgctcgat aaaacggctg gagtggttga tttatctttt taaataagtc aaagtaatcc
    30361 tgtctggtcc ttaatagtgg tactggatgt ttcattgtat tttctcctct ttttactacc
    30421 aaatagctaa ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa taatcagaat
    30481 agatcagtgg cacattataa gtcagttctc tatggtatgc ttctgctccg cctccaatga
    30541 tcccatcttc atccatattc caatttgcat agtgcttttc tgtcttttgg agtatattga
    30601 tagcagcttc atgatacatt tttgatcgta cagaggaaac atatcctgct aattccagaa
    30661 gaccgcatgc agcgcatact ccagcactgg tatcaatatt cgaatctttt acagcaaaat
    30721 caatgagagg aatataacca gttttttcga ttttctgaat aaaatttact gctatctttt
    30781 ctgctatagc tagatagtac ggattttttg tgtaacgata acttaacgcg aatccatata
    30841 aagcccatgc atttcctctg ctccaacaag aattctcatt gtatccttgc ccgccaagta
    30901 accgaatata tttccctgat tctgcatcaa attctgcgat atgcccggtt gatccatctt
    30961 tcctcactag atacttagct gttgtatctg catgctgccg agcaatttta gagaacctcg
    31021 gatcaccagt ttccctcgaa gcccaataca gtaatggtaa attcatcata ctatcaataa
    31081 tcatccaacc aggtttatgg tcattccatg ctactaaata gttccctgaa atattatagc
    31141 gtccagcaag taccgtagct gcatgtaacc ctagttgtcg actatgtttg ttttgagact
    31201 gttgataatg tgcaatagcc gatggtaaga ataaaaaacc tacatcatga tgaacgtctg
    31261 aaaaatggta cagggcagat tctaataatt gttcttgttg tattgctgcc tctttgaact
    31321 tttctcgttt tgtcttgtga aagagctgcc ataaaatacc actccaaaaa ccattggtcc
    31381 accagtcgat tcctctgtcc tttattacgt ctgtatattt tccgttgtaa gaaatatatg
    31441 gaattttatt ccccattctg tctaattccg catctatctt ttcagaagct acattcaaaa
    31501 tttttcttga ccattccttc gtctgttcat tcatggatat cccgccattc ttttaatttt
    31561 tatgaatgtg gagttgctcc tagaacgaac tagatggtta acattcgtta ttttttttac
    31621 ttatttgcag ctccaatgtc attctcctta aatttagact gcgcccacac ataaatcaag
    31681 accattggga tcactgaagt tgctaaaatt agcataattg cgccataatt aattggctca
    31741 cctgtactag aaaccgtcat atttttgata attaagggaa ccgtatattt atctttatct
    31801 gaaagcatga ctaatggaaa tagatattga ttccaacttc ccataaagat aagtaaacca
    31861 ctagtaataa tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat cacccactca
    31921 ttggcaccat cgattctggc agcctccatc agtgctgtag gaaattgact gctatattgt
    31981 cgcatcataa acactgaagt tgtactggca atcgaaggga tgatcacacc tgcataagta
    32041 ttcgataatc ctaaacgact cataatgtaa aataaaggaa tcaatgtggt aaaaaaaggc
    32101 accatcctag atagcataac tgtattgaac aacagtttat tccccataaa acgatacttt
    32161 gctaatgcat aacccgccat tgtagaactg gctgtagcta aaactgtact gattacagca
    32221 ataacaatcg aattccaaat aatcaaggga taattatatc tcgccatgat ggcttgccag
    32281 ttttttaata gcatatcccc aaacttgaaa ttgattccgc cacttaaaat ttcttgatta
    32341 ctttgcgttg aagctacaat catatataaa tatggcatca aaaatattaa ggcaccaaac
    32401 acaataatga cgtacgtaaa aattctgctt tgtatctttt gtttagtcat ttttatcacc
    32461 tccgaattta aattgaatag ccgataatat aacagataca aacaccatcg tccaggcaat
    32521 agcagaggct tttccaaaat caaccatttc aaaacttgtt ttatataaat tgactcctac
    32581 gaccaaagct gaattttctg gtccattgga agtcccaaag aatatatttg ggatattgaa
    32641 attacccaag ccattgatag tggctaaaac tgtggccatt aaaatgattg gctttagcat
    32701 ggggatcgtt aataaaaaga attgccgaaa tttcgatgct ccatctattt cagcactttc
    32761 gtatatctct ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt tatatcctac
    32821 actaagccaa atcatcgtga tgatcattga tattcgtatc caaaaaggat ttgttaacca
    32881 ctgtacaccg ttaattccta gcgcattcaa aaatccattg aataacccag tcggattgaa
    32941 aaaagtcatg aatacggaag ctacagccac cgttgatgta atggttggaa tataatagac
    33001 agttctaaaa aatccttttc cttttaactg tgctgaattc aataaaacag caaagtaaat
    33061 agctaatagc atcgttattg gaattgatcc tgccatgata atcaaaacat tcgtgtaaga
    33121 tttaataaac aatggatctc ggaagatagc tagataattg tcaaatccaa caaattcaaa
    33181 tgagtagcca attttcataa aactaaaagc aaacgagata atgatgggaa tcaaaatcgt
    33241 cacaataaag aaaataacaa aaggtgtgac caatccataa gcactttttt tatgagacgt
    33301 atctgttgct tttttcttgc tgttctttat tgtcttgctt ggtaacgaag attgcatcat
    33361 cgttaagtcc tcctaatatt ttttaatcac cagttaattt tacgccgtac ttatgagcaa
    33421 aattttctgc ttcttttaga tatgaatttt caaaatcacc tgtttgccaa tagttatatg
    33481 cagcggtagt caaataagac gtagcatctg aataagtggc tggtaaaaca gtatttgatg
    33541 tattttcatt gattttattc aatagttcat agtatttttg tcctccttag tagtcaaact
    33601 ttttctcagc tgcttctgtt tgataggcca atgagttaga tgtagccacg cccagctcca
    33661 gtgcgttggc taagcattct tcatctgtca gcataaatgt aagaaattgt tgtgccactt
    33721 gtggattttt tgcatcttta gggacataaa aacttgatcc tcctgatact gcaccttttc
    33781 ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca gatgcatcag
    33841 gaaaattcaa agacatattc gtagcatacc agccgccagc aacaaacatt gcagattctt
    33901 gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac aatatctgag tcgatcattt
    33961 ctttgattaa ctttgcagca tgtttcgctt ctttagtatt cagacggata tttccttctt
    34021 catcaagcaa agattgattc tgctgggcta gcatgttgat aaacaattct tggtccccag
    34081 attctggcaa ggcaattcca aaactaccag ttttttcttt taatgctttt gccacctgga
    34141 tatattcttc ccaagaactg atttcttcaa aatctacacc tatttcagca aatttttttg
    34201 gttgataata acaaagtgct ggggtaaaat cactagccca aggaataatc gtttcattac
    34261 tcgtttgttg acgcaaaata ttcaattttt gatcataaaa ctgatcgcca tatttgtcat
    34321 aaaatccata ttcatcagca ctataaaaag catcttgaaa tttttccagc acacctgctg
    34381 ctgaagtgtc tgccatggag ataatatccg gcatatcttc gtgtgttacc aattttgggg
    34441 tgatcatttg tactgttgct gttcccgaag ctacattcac aatttttaag tcaatatttg
    34501 ctccagtttt gcggttatag atttttgcag cattttcaac atagtttgtc agtttgccgt
    34561 ttgcgacata tacttctaat gtagtggctt tttttatcat tggatctgcg atgtttgctg
    34621 ttacttcttc gttagttttt ccacatgcac ttaagctgaa taatagggta atgtttagca
    34681 aagctatcac caatttgtat ctttttttca tgtctatccc ttctttcttt tcaagttctt
    34741 atataaaacg ctttcactta tgtatattaa cggaattagt aacacaaata tttatataaa
    34801 aaaatatttt acttgtcgtt ttggcttttc atttttgtta taatagtcat taattataaa
    34861 tagaaagctt tttatataaa aagagaaagg aaagtggttc ttttgttaac aaacaaccaa
    34921 tcctccaatc ttgtcaaaaa aacactaacc tattccatag atacccatct tcatttatct
    34981 gctgctccct attttcgact actaa
//
LOCUS       DAZKOR010000019        34588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000019 DAZKOR010000000
VERSION     DAZKOR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.019"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..974
                     /locus_tag="ACG8ZM_000874"
     CDS             144..974
                     /locus_tag="ACG8ZM_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0491855.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(1218..1467)
                     /locus_tag="ACG8ZM_000875"
                     /pseudo
     CDS             complement(1218..1467)
                     /locus_tag="ACG8ZM_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1658..1852)
                     /locus_tag="ACG8ZM_000876"
     CDS             complement(1658..1852)
                     /locus_tag="ACG8ZM_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491856.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(2357..2542)
                     /locus_tag="ACG8ZM_000877"
     CDS             complement(2357..2542)
                     /locus_tag="ACG8ZM_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491857.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(3392..3733)
                     /locus_tag="ACG8ZM_000878"
     CDS             complement(3392..3733)
                     /locus_tag="ACG8ZM_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0491858.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(3733..3963)
                     /locus_tag="ACG8ZM_000879"
     CDS             complement(3733..3963)
                     /locus_tag="ACG8ZM_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0491859.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            4250..4588
                     /locus_tag="ACG8ZM_000880"
     CDS             4250..4588
                     /locus_tag="ACG8ZM_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491860.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(4793..5355)
                     /locus_tag="ACG8ZM_000881"
                     /pseudo
     CDS             complement(4793..5355)
                     /locus_tag="ACG8ZM_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(6087..6668)
                     /locus_tag="ACG8ZM_000882"
     CDS             complement(6087..6668)
                     /locus_tag="ACG8ZM_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0491861.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(6851..7477)
                     /locus_tag="ACG8ZM_000883"
     CDS             complement(6851..7477)
                     /locus_tag="ACG8ZM_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0491862.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(7499..8830)
                     /locus_tag="ACG8ZM_000884"
     CDS             complement(7499..8830)
                     /locus_tag="ACG8ZM_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0491863.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(8985..9386)
                     /locus_tag="ACG8ZM_000885"
     CDS             complement(8985..9386)
                     /locus_tag="ACG8ZM_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0491864.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(9472..9678)
                     /locus_tag="ACG8ZM_000886"
     CDS             complement(9472..9678)
                     /locus_tag="ACG8ZM_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491865.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(9773..11275)
                     /locus_tag="ACG8ZM_000887"
     CDS             complement(9773..11275)
                     /locus_tag="ACG8ZM_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0491866.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(11293..11748)
                     /gene="argR"
                     /locus_tag="ACG8ZM_000888"
     CDS             complement(11293..11748)
                     /gene="argR"
                     /locus_tag="ACG8ZM_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0491867.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(11846..12463)
                     /locus_tag="ACG8ZM_000889"
     CDS             complement(11846..12463)
                     /locus_tag="ACG8ZM_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0491868.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            12684..14057
                     /locus_tag="ACG8ZM_000890"
     CDS             12684..14057
                     /locus_tag="ACG8ZM_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0491869.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            14132..15244
                     /locus_tag="ACG8ZM_000891"
     CDS             14132..15244
                     /locus_tag="ACG8ZM_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0491870.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(15316..16953)
                     /locus_tag="ACG8ZM_000892"
     CDS             complement(15316..16953)
                     /locus_tag="ACG8ZM_000892"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0491871.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(17121..17729)
                     /locus_tag="ACG8ZM_000893"
     CDS             complement(17121..17729)
                     /locus_tag="ACG8ZM_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0491872.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(18113..19264)
                     /gene="queG"
                     /locus_tag="ACG8ZM_000894"
     CDS             complement(18113..19264)
                     /gene="queG"
                     /locus_tag="ACG8ZM_000894"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0491873.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(19283..20035)
                     /locus_tag="ACG8ZM_000895"
     CDS             complement(19283..20035)
                     /locus_tag="ACG8ZM_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491874.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(20176..21125)
                     /locus_tag="ACG8ZM_000896"
                     /pseudo
     CDS             complement(20176..21125)
                     /locus_tag="ACG8ZM_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            complement(21126..21965)
                     /locus_tag="ACG8ZM_000897"
     CDS             complement(21126..21965)
                     /locus_tag="ACG8ZM_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0491875.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(21962..22708)
                     /locus_tag="ACG8ZM_000898"
     CDS             complement(21962..22708)
                     /locus_tag="ACG8ZM_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491876.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            22946..24316
                     /locus_tag="ACG8ZM_000899"
     CDS             22946..24316
                     /locus_tag="ACG8ZM_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0491877.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(24371..25267)
                     /locus_tag="ACG8ZM_000900"
     CDS             complement(24371..25267)
                     /locus_tag="ACG8ZM_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0491878.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(25381..25551)
                     /locus_tag="ACG8ZM_000901"
     CDS             complement(25381..25551)
                     /locus_tag="ACG8ZM_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491879.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(25548..26225)
                     /locus_tag="ACG8ZM_000902"
     CDS             complement(25548..26225)
                     /locus_tag="ACG8ZM_000902"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0491880.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(26316..27221)
                     /locus_tag="ACG8ZM_000903"
     CDS             complement(26316..27221)
                     /locus_tag="ACG8ZM_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0491881.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            27396..28820
                     /locus_tag="ACG8ZM_000904"
     CDS             27396..28820
                     /locus_tag="ACG8ZM_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0491882.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            28866..29897
                     /locus_tag="ACG8ZM_000905"
     CDS             28866..29897
                     /locus_tag="ACG8ZM_000905"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0491883.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            29904..31091
                     /locus_tag="ACG8ZM_000906"
     CDS             29904..31091
                     /locus_tag="ACG8ZM_000906"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0491884.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            31247..32629
                     /locus_tag="ACG8ZM_000907"
     CDS             31247..32629
                     /locus_tag="ACG8ZM_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0491885.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            32666..32986
                     /locus_tag="ACG8ZM_000908"
     CDS             32666..32986
                     /locus_tag="ACG8ZM_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491886.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(33186..33854)
                     /locus_tag="ACG8ZM_000909"
     CDS             complement(33186..33854)
                     /locus_tag="ACG8ZM_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0491887.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(34043..>34588)
                     /locus_tag="ACG8ZM_000910"
     CDS             complement(34043..>34588)
                     /locus_tag="ACG8ZM_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0491888.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
BASE COUNT         9894 a         6985 c         6227 g        11482 t
ORIGIN      
        1 aaaataacaa tttataaggt atgattctaa aaaaattatt aatgatacca atactttata
       61 taattcgcga gcgctttctt tgaatttttt aatctttcgt agcagaatga gaatgtatta
      121 aaaatagatt aggaggaatt ttgatgttta gacaccagaa agaattgcag tttgaagcaa
      181 aaccagatcg tccgaatcca ttaatcgcga aatatctgca ggagctgatc ggcggccaat
      241 atggcgagat gagtgtagct atgcagtacc tgttccaagg ctggagctgc cgaggagaag
      301 aaaaatataa agatatgctg atggacattg caacagaaga aatcggccac gtggaaatgt
      361 tggcgacaat ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa gaagcagcga
      421 tgcaaaaaga tccaacaatc ggtgccatta tctctggaat gaatccacag cacgcgatcg
      481 tagctggctt aggacctcgt cctgctgaca gcgtgggcaa tccatggagc ggtgcctata
      541 tcattgcgag tggcaacttg ttagcagact ttagagctaa tttaaatgcc gaatcacaag
      601 gaagacttca agtagcacgt atttacgaaa tgatcgatga tcgtggcgta aaagatatgc
      661 tgtctgttct ccttgcaaga gatagctatc atcaaaatct atgggaatgt gcagtgaaag
      721 aattagaaga aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt gacaaagaac
      781 gaaaagacat tgcttatgac ttctataact tctcagaggg agaccaaagt agtgaaggtt
      841 cttgggctag tggcaaagca ctggatggcg aaggcgaata tcaatggttg aaagaaccta
      901 agatcgaagg cgctgcacca aaattagaac cagtaacacc aaaaatgtat gggacacctc
      961 cattcaagaa ataaatatct acccgtagaa acacccgttt tttgttccac acgctttgtg
     1021 agcaaaactg actaaagtca ttaatcataa ccacccttca aaagggtggt tttctctaga
     1081 gctataagct caaatcaccg gccagcgcct aaagacgctg gctttcatat tttatgttca
     1141 agccatattg cctttgccag ttttgcccac ctcttaaaag gtttatgtac tactccttag
     1201 cccttaaaag gatcgtctta ctctcgtaca ctcagcttat ctaacgcact atcatgtttt
     1261 tcttgttctt gaatgtactt tttgatggtc gcttcatttt gtcctacttt tgtcacatag
     1321 tatccttcca cccaaaagtg tcgataccca aatttatatt ttagatttgc acaatctctt
     1381 gatgatctcc tgaatattgg ctcgatattg attataaatt atttttcttc tatactttgg
     1441 tgtgaataca atgtggtatt tacacatcta ctttgtacgg gctaaactat ttccctttat
     1501 tgctccaccc gcatagcaag tggttttgta tttcaatcgc tcggcgcgct caattaggtc
     1561 taaagacaat aaatctaaaa acccgctgct tcactgacag aaacaacggg ttttcagaaa
     1621 aaaatgaaat gaaaaagatc tcttacggca atctatttta tttttttgat tttttataaa
     1681 tggaaaatgc ttcaagaagt ccaagaaata aagccgtata aaaacaagaa cctatgaaat
     1741 ctttatttat tagatattca acagaaatcc cgataacact agccaacaat atatttatca
     1801 gaagtgtaaa ataaggatgc tttttataga atagaactag tgttgttttc atagactctc
     1861 ctttcttcat atttccaatt aatcacaaat tcacggcatt actagaaaaa ttaaaggggc
     1921 gcagaagtgg aaaaatactc ttcgactttc cctacaaacc aggccatctt gtgtttaata
     1981 actataccaa atctaaggta cctctaaatc taaattccat ttgtggcggt gacctccaat
     2041 ttttaactct tctatctccc atccagtata cataaccttg atacttcgac cacttatata
     2101 tcgaacacac aaatacctat ccattgcagg agtactacct aacttagttg ctacttgcat
     2161 ctaaataaca gctgaaaaga taggacttac tcttagtaaa gtatctgaac tattaatttt
     2221 taaactatca tttttatcct caactacatg cacatctgtt gcaacttcac tttctgtctc
     2281 atccagataa atgattaagg aatcgggaat tgcaatatct tcagctgacg ctggaatagt
     2341 tgagctaaat tagtcatcat atctttatta taaaataacc aacagcaaaa aatacaatga
     2401 aaaacaaact tggaactaat gtatcattaa taaatgttga aaaattcagc aactcattac
     2461 tgtatgattt tagcagccca accaacatat accctataaa agctaataag atggttagga
     2521 atcctttaga atttttactc attcatagac acttccttaa ataactttta ttatagaagg
     2581 tattgtaaat aataatattg acaaaaagtg actcaaaata aaaaaaacct tacattatac
     2641 tggaatgaac tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg
     2701 gtaaagtaat tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa
     2761 aggtactttt acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc
     2821 aattgcacat tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta
     2881 gcggtagatt tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt
     2941 tctcttttca ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta
     3001 tagaaattct ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata
     3061 gtttaaaatg acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag
     3121 ttgttctttt tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat
     3181 gtcgtgaaaa aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa
     3241 tcattcgttt aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac
     3301 cacactagta gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat
     3361 ataaaaatat tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa
     3421 ttttagccat gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat
     3481 caagagattt taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg
     3541 agtaacgtgt cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg
     3601 ttttacggtt aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt
     3661 ttcctacgga gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc
     3721 tttcttctga cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt
     3781 ctcataatac tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt
     3841 aattgttcca tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc
     3901 gttatcagaa ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag
     3961 cattatcatc actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc
     4021 ctttgtaaac tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga
     4081 aataaattgt agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa
     4141 cctatggatc caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc
     4201 agataaaacg ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta
     4261 taaacaaaaa gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa
     4321 actagcaggt gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac
     4381 attggttcaa cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc
     4441 tatttttgta gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca
     4501 acctcttatc tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt
     4561 tagattccaa gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga
     4621 ctcgcagcac gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct
     4681 atagatgcag tatatgcagc ttatgagcat ttcttctgtt ctctttgttt ttgggatctg
     4741 tgctgaagtc tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct
     4801 tctccattac taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg
     4861 ttacccaagg ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg
     4921 cgactgtgcg gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat
     4981 ggctacgata aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt
     5041 ctttttcttc attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa
     5101 ggtctttttt tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa
     5161 tccggtaaat ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa
     5221 cgtcacaaca agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg
     5281 ttgtttcgtc tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa
     5341 ataacgacca cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga
     5401 agttcgcacc aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac
     5461 aaactctcca gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa
     5521 caaacatgag aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct
     5581 tacgacctct gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg
     5641 caaaaatgta gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt
     5701 ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
     5761 aaacgaaaaa tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag
     5821 ctttttcaca acctctccac taaacggtgt tcaatcagct aaccttcggt attagctcaa
     5881 aaaacggcaa aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc
     5941 gctgatttca caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc
     6001 gaaatttcac aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg
     6061 accacttctg tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct
     6121 ttgatcaatt gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag
     6181 atacttccct ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggac
     6241 agtttttctt cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact
     6301 aattctagta gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta
     6361 cctacaggac agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac
     6421 atttcttcta atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct
     6481 gtctgccata cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa
     6541 aagtaataat aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac
     6601 gtttcatgat agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt
     6661 gttttcaatg atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat
     6721 cattttttct tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac
     6781 tataaaactg ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc
     6841 aattcctatc ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa
     6901 taaagccaaa gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat
     6961 ctgtcgctac cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac
     7021 acaagtttcc gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga
     7081 gcgccaaatc attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt
     7141 catccaaata aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt
     7201 catattgact ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc
     7261 caaattgcca tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc
     7321 ctttttcttt tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta
     7381 agatatcttt tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt
     7441 cagtgagaac tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt
     7501 attttaatac atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca
     7561 ataacgtata aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag
     7621 aatcttccgc atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt
     7681 tgaatacacc tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact
     7741 tgaataattt gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg
     7801 ataaagtcgg agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt
     7861 tattctcgcc cttcaactga tccataatga ttcgataatc atctagagag atataagtga
     7921 actgcaatcc gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt
     7981 attcatccac agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa
     8041 gtgatgctgt acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta
     8101 cttcttggcc atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat
     8161 ctacagatac acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt
     8221 gcgcgatatc ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt
     8281 tagatccgta ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga
     8341 tcactaattt ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc
     8401 gacttgtatt caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa
     8461 tccgttcgcc ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag
     8521 aaagaaaacg tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat
     8581 aatttttggc gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt
     8641 cgattcctct ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac
     8701 atgttccgcc atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag
     8761 ctaacgcctt cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt
     8821 tttccatcat ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac
     8881 cgataattgt ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag
     8941 cggtcctttc acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact
     9001 tctacatcga tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct
     9061 gcaatttttt gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg
     9121 atctgatagc cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct
     9181 gctgtcattc ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa
     9241 cctgctgcaa ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt
     9301 ttcatatgca tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc
     9361 attgctgttt cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt
     9421 ggctataatt atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa
     9481 taccagaatg ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa
     9541 cagcgtcata tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt
     9601 gattttaaga tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat
     9661 aaccatattc gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta
     9721 accataccac tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg
     9781 ctgttaatga ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca
     9841 acttgttctt tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc
     9901 ttaaacggat caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt
     9961 cttcattttc cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt
    10021 ctaatatctt gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt
    10081 gtacttctac gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt
    10141 ttaattctgg caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat
    10201 agttttcctt gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa
    10261 gacggttaag ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt
    10321 ggatgcctcc tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca
    10381 cgattcttgt tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat
    10441 ttcgattgat cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga
    10501 aaatgatccc aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt
    10561 ctttattatc aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg
    10621 aacctgttga atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac
    10681 catttccggc atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa
    10741 tccccttttt aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt
    10801 ctacgcttcc agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat
    10861 cttctttttc tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa
    10921 gatggctagc agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca
    10981 taaacatagc aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca
    11041 gactttggat caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc
    11101 caatcgtcca ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta
    11161 gtccccagcc gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg
    11221 gtaatgcgat tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa
    11281 agtcctcctc atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc
    11341 ttctgagcgg caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg
    11401 gtaagccact tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac
    11461 gataaccatg aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa
    11521 acgttcttca tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt
    11581 ttcattttga ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt
    11641 cacatcggcg cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg
    11701 ttctgtaatg atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc
    11761 tcaatgttga ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt
    11821 ttattttttg ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct
    11881 tcattttttc aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag
    11941 aagctctttc atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt
    12001 cttcggcata gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga
    12061 atactacttg ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg
    12121 ctatcgcttc tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt
    12181 ttttcatcac tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat
    12241 agacagtata catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg
    12301 catagctgac tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc
    12361 cgatccctct gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt
    12421 tcatactata gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa
    12481 attccattta gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac
    12541 actatttttc agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt
    12601 cggcgtgtcg agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac
    12661 gaaatttttt agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag
    12721 taattgcaac agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa
    12781 ctttcccaac tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa
    12841 cgatttatct tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt
    12901 ttagtctcaa aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg
    12961 atttcttctc tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga
    13021 caggtatcgc tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac
    13081 gaggcgcgat gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca
    13141 cttatggagg cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc
    13201 ccgttttgtt tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa
    13261 aaagtggtta tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc
    13321 taacattttt gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta
    13381 ttggctggtt ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt
    13441 ctattctttc aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt
    13501 tattacttgg tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc
    13561 cttttgtcgc tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt
    13621 tttccgggaa aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc
    13681 tttctttgat tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat
    13741 tgattgcttg tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga
    13801 ccaccggaat gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata
    13861 ccttgcagca gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg
    13921 tccagcatca tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat
    13981 taggcgtctt gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct
    14041 ttgctaaaaa agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg
    14101 aaaatggttg tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg
    14161 gatcattggt atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt
    14221 tgatgaaggg acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc
    14281 gaaacgcaga aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa
    14341 acttataaaa gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct
    14401 tcgacctgaa aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc
    14461 gccagaaatt ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt
    14521 attgaaacca gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata
    14581 tttagaacat cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt
    14641 cactttgcat aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc
    14701 tgatcgtgta attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta
    14761 tgctgcagat atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat
    14821 gagtcaagac tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga
    14881 tggaaagatc cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac
    14941 tagcgaagct tataagggat tgactaagag tacagaacag ttcttgactg atccagatca
    15001 gataaaaaac tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc
    15061 cccatttttc ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc
    15121 ttctggcttg accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg
    15181 tcattggcct acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc
    15241 atagaaaaac agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt
    15301 gaaaaagctg ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc
    15361 atctggcaag agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg
    15421 ttgtttcacc agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg
    15481 taaatagtgg atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat
    15541 tctttcaacg attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt
    15601 attcaggaca tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc
    15661 ttccctaaga ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag
    15721 ctgtcttttt gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa
    15781 agacgctgct aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat
    15841 gatccaaaaa acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata
    15901 acgtgatttg actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc
    15961 tcctttactt gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg
    16021 atcatcagac ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa
    16081 accatttttt cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc
    16141 cgtatgtttt gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat
    16201 cgtataggtc tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc
    16261 attgcgattt cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta
    16321 acgtaatcct agcacagccg ctgtattatg gagagcatca aacaaaatga gtccatttgg
    16381 aaatgtttca tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga
    16441 aacagacttt tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata
    16501 agaatacaaa ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc
    16561 ttcagatagg ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac
    16621 agcttctctt aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg
    16681 ccgagcactt gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc
    16741 agctggtaaa tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg
    16801 actccctttt gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc
    16861 gtaaaatgtc gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat
    16921 ttcgtttaat tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa
    16981 aacgttttct ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc
    17041 aaacgagtta tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc
    17101 atcattttcc ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca
    17161 tcccatgaac cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct
    17221 gaatagtcgc cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta
    17281 gtagctgtgt atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa
    17341 gttggcgctg ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca
    17401 acgtatgtat gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg
    17461 ttttctgttg ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa
    17521 atgcggttga tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct
    17581 cctgcaaatt tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca
    17641 tcagcgtgag catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta
    17701 cctaaaacaa ttttcttaaa tgaattcata ttaaactcct ttttttctcc gttttttatt
    17761 ctttcgacgc tattaacttt atcatttaga aatatttcat aggtaaaaga aacatgacat
    17821 ttaatgacaa aataacgaat tatttaatat ttgtaacatt taaacaaata tttttttgat
    17881 acaaaaccat aaaagccttg ttgtataagc aaagtaatcg tttttctatg tttttttatt
    17941 tttgaatgaa aaacgcagat tctgtaacat tcactgcaat aatccataaa aaaaagcaaa
    18001 aaaatttttg atttttatca aattctctta aaaaaatgta aaaaatacaa aaaaaccagt
    18061 aattgttaca acaattactg gacaaaaact ttgaataatg ttaactattt ttttatttta
    18121 actttctccg caataatttc tgggcatgtt ccaattcgtt tataacgact tcttctgttt
    18181 ctttagctaa tatttcatct aatacatcaa gccatttttc cggttctttt gttcctagac
    18241 gtccaatagc ccaagctgct gttcctctga tgacagggcg gacatctgtt aaacattctg
    18301 aaatctcagg caatgccgtt cgatcaccta gattagctaa agcaatcaat gcgtttctct
    18361 gcaaaggttt cttcccacgc catgaaccag ctaaatgccc aaattgctgt ttgaagtctt
    18421 tattagacat cgaaagcatt ggctttaatt ttggatatat ttcgtctact tttggttcca
    18481 tctcttcatg aaaatgaaaa tcttttcctt gattgtaagg gcagactaac tggcagatat
    18541 cacatccata aatgacattt cgcatttttt tacgatattc ttccggcatc atcccttttg
    18601 tctgagtttg ataagacaaa catcgctggg cattcattct tccgtcacca agcaatgctt
    18661 ttgtcggaca agcagtcacg cagcgcatac agtcaccgca accaaattct cctggtttat
    18721 caggctcaaa tacaatatta gtaacgatct caccaagata gacaaatgaa ccgaactctt
    18781 cagtgatcaa caatccgttt cgtccaataa atcctaaacc agctcgctgt gctacagcga
    18841 catcgatcag ttctccagta tctacttgcg gagctagacg ccattcctct tctttttgcc
    18901 atttttctgc tcttgtttgg atgaattcga tcaatcggtt caaacggtct ctgagtatat
    18961 catggtaatc gatcccccaa gaagcacgag caaactgccc gcgtttttca tccctaggca
    19021 cttcttcatg gatcctagta ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat
    19081 caaatgtcat ttctggataa agacgctcat cgatatttgg atgttcaaag ccagaagtat
    19141 ggccagctgc tttttgttct tccaacgatt cttttaatga atcaaaaggt tccgccgtgg
    19201 tgaacccgat tttgtcaatt cctaatcgtt tgctttcttg tatgatttct tcttttaatg
    19261 tcatggtctt cctcgcttgc tgttaacggc tgtataaccc ataatatagt ttttcttttt
    19321 tcagctggac aaactggatc tgctgatcgg caatcttctt ttcttccatc catttttttc
    19381 ttgcagcaaa tacttcccaa taatttgttg aaattaattt tcttttgtta tctaatgcat
    19441 taaaaaaatt caatgaaact ggttttgtac ttaacagttc atggagcctt acctcttccc
    19501 atggcgaaaa ctcagacaga tcgcgggaat acaatacttc ttcatagaga tttttttgct
    19561 gtgtgtacgt gaatgtttgc gggattttcc caggcctagc aatttctact agacgattat
    19621 aaaacatatt cattaatttg ctgttggcaa taaattgact tttataatag gccgactgga
    19681 tctttttgat tactccttct gctttgatga ctgaaaataa tgcaggccat gctttgacgg
    19741 ttatttcgtc gttttgttta actaatacgc caggtgtgaa gccgactgga gaaaggaaca
    19801 caaaataacc gataccggta ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc
    19861 ctatgtacag ctcttcatct ggttctgttg tctcattttg atttgctatg aataatttag
    19921 ctaattctgc ttcaagaaat tgaaggtctt gatccttgtc ggtattttcc tgctggctga
    19981 tcaatcgaaa gacttcttct ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca
    20041 caccttcctt ccttactcta actataacgg aatttcttca aaaaaggtat ctgtacgacg
    20101 aaaaacagca taattttttt tcatatctct tataaaaaat gcaaggtctg ctcctcaagc
    20161 tagaccttgc acttttcagc tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat
    20221 attccatttc atcatctgat agtaagaatc tccttgttct ccttcttttg ctatggaatc
    20281 agtgaacagt ttatcataga ttttcaaacc cgtttctttt gctacccgtt ccatgcttct
    20341 tgagtctaca ctggtttcca cgaataaaac cggtacttct tttgctctta tctgatcaat
    20401 gatcgctttc atttgatcag gcgtaccttg actttctgta ttgatctccc atatataagc
    20461 tgctggcaaa tcatatgctc gtgaaaaata cttgaatgcc ccttcacttg ttactaataa
    20521 ttttttgttc tcttcgatag aagcaaaact ttccttcgct tgtgtatcta attcttttag
    20581 tttttccaca tatgcttctg cattttttta tagagtgtcg cattttctgc atctttttca
    20641 gagaatatac gtgcgatttc ctccacatat tttattccgt tagataggtc tagccaagca
    20701 tggggatctg cttttgtatg ttcttcaccg ctagttaaat aaagaggttc tacatttttg
    20761 ctaactgcaa aataatcttt tccttcttct tttttagccg tttccatcaa gttatcgaac
    20821 cagctgttac ctgtttcaag attcaaaccg ttgtataaaa taacatctgc atcgcttgcc
    20881 tttttgatgt cttctggtag tacttcatat tcatgcggat ctgttccgac tggtacgata
    20941 ctgtggatat ctatttgatc tgttccgacc tcttttgcca tgtccgccaa gatcgaattg
    21001 gtagccacga ctgacaactt ttctttactt ccttcatccg cttggtcaga agcctgcttt
    21061 ccgcaaccgg ctaataccaa gaaactaact gccaagatta aaaagagtga tttttttgtt
    21121 ttcatctatc tgcttccttt ctgaaataaa attccttttg ttggagaaaa taaaaaagca
    21181 aggataaaaa atgcggtaca tgctaacacg atcgtagctc ctgatggcca attgtaacta
    21241 taactgaaaa acacaccgac aactgcactt agcataccga ttcctgcagc tgtaatgatc
    21301 attttcagta aatggttcgt tagcaagtaa gccgtggcgg ctggtgtgat caacatggca
    21361 atcaccaaga ttgttcctac tgtctgtaaa ctgactacag ccactaatgt caaaaagaac
    21421 atcaaaagat aatggatcag cgatgtgttc agcccataag cttttgccat tgtcggatcg
    21481 aatgatgtga tcttcaattc tttataaaag aaaaagacaa atatcaagac gattccacta
    21541 acgagtgctg tcagcaacaa atcgctttcc ctaacagcta agacattccc aaataaaata
    21601 tgatacaaat ctgtcgcact ctgggcatat gaaatcaaga taatccctaa tgcaaaaaag
    21661 gagctaaaga caattccaat cgctgtatca tttttcaatc ggctccgctg agtcacgaaa
    21721 ccaatcactg ctgctgttag catcccaaaa gcagtggctc caaagatgta actaaaacca
    21781 aacatataag aaatggctac acctggaaga actgcgtgag agatagcgtc tcccatcaaa
    21841 gacatccctc gtaaaataat aaaagaaccg atgactccag aagcaagtcc tacaagcatg
    21901 gaagtgacca aagcgtattg caggaattga taattcatta atccatcaat aaaggattgg
    21961 atcatttttt ctctcctcct tttactaaat gtctgatgat ctcaccatag gccaattgaa
    22021 ggttcttcga tgtaaagact tcttgtactg gaccggaagc gatcagctgt ttatttaaaa
    22081 gaataacctc atcgaaatat tcttctactt tatgaagatc atgatggacg atcaaaatcg
    22141 tttttcctga attcttcaat tcctgcaaga tgtcaaagat ctttcgttca cttaacgcat
    22201 caatccctac gaatggttca tctaaaaaga tccattctgc tccttgggct agagctcttg
    22261 caataaaaac tctctgaagc tgtccacccg atagttcgct gatctgtctt ttggcatatt
    22321 cttccatccc tacttttttc aaagcttgtc ttgcacgttc tttttctttt ttcccaggtc
    22381 tttgtccaat tcgtaaagat ggatatgttc ctaaaagtac aacgcctaat acatcaattg
    22441 gaaaggaaag atccaattca cttcgctgtt ccacgtaggc tattttttta cggactgagt
    22501 ttaccgactg tccagaaaag gtgactttac cagtagtagc tggtatcaac tctagcaaag
    22561 atttcataaa tgttgatttt cctgcaccgt ttggtccgat aataccggta attttttgtg
    22621 gatttatcgt caagttgata gttttcaaaa cagttttccc ttgataagcg gctgaaaggt
    22681 cttgaattac aatggccgta cgttccaaat cttacactcc tttttaggtt tacctaactt
    22741 tttatttaaa tatagtttaa ctaaaatgga aagcattttc aagtagaatg atgaaaaagt
    22801 ttttttcgaa ttttcctcgc attttatgaa aattttctgt agaatcacat caagagttca
    22861 ataaggctaa cctaataatg aaaaacatgt gttgaaacgt ttgattattt tttcttgttg
    22921 actcaacaac gaaaggagtt tttgtatgga agaagaaaag ttaactcgaa gtcttagtgc
    22981 ccgtcatatc caaatgatcg cactaggtgg aacgatcggt gtcggtctgt ttatgggtgc
    23041 ctcctcaacc atacgttgga cagggccctc agtgatggga gcctatgcga ttgcaggatt
    23101 ctttttgtat ttgattatgc gtgctttagg cgaaatgctt tacgttgatc cttcgactgg
    23161 ttcttttgca aattacgcga gcgagtacat ccatcctgtg gctggctact tgaccgcttg
    23221 gagtaatatt tttcaatata ttgtcgtagg aataagtgaa gtgattgctg taggatctta
    23281 tatgaattat tggtggcctg atttgccggc aattattccc ggaatcattg tcgtcttgtt
    23341 tcttatgctg gcaaatctta tttctgtcaa ggcgtttggc gaattagaat tttggttttc
    23401 gatcatcaaa gtcataacga tcattttgat gatcattgca ggtcttgggg taatcttatt
    23461 tggatttggt aatcatggtc aggcagtagg gatttcaaat ctttggaaaa atggtggatt
    23521 cttcactgga ggcgtcaaag gatttttctt tgcattatcg atcgttgttg cttcctatca
    23581 aggaatcgaa ctgatcggaa tgactgctgg tgaagcagag aatcctaaaa agacgatcat
    23641 tgaagctgtc cagtccacta tcggtcgaat cttgattttt tatattggtg ctatttttat
    23701 cattgtgagt atctatccgt ggaatcaatt gagtgagatt ggttcacctt ttgtacaaac
    23761 tttttctaaa gtcggcatta catttgctgc aggactgatt aattttgtcg tgattacagc
    23821 ggcattatca ggttgcaatt cagggatttt cagcgcaagt cgtatggtct atacacttgc
    23881 cataaatgga aagatgtcta aaaaatttct gaaactatct cgtcacggtg cgccttttta
    23941 tcctgtcgta gcaatctcag gtggaatctt aattggtctg atcttaaatt atcttctacc
    24001 aatgttcatc aaccagtcag aaaatatttt cgttttcgta tacagctcaa gtattttgcc
    24061 tggtatggtt ccttgggtag tgattttgat cagcgagatt cgcttcaaaa agaagcacgc
    24121 agataaaatg catgatcatc cttttaaaat gccactatct ccttatacga attatgtgac
    24181 acttgctttt ttagctatcg tattgatttt catgtttatc aatcctgaaa cgagaatctc
    24241 attgattatt ggactcgttt tcttgcttta catgacgatc cattactttg tacgcgaaaa
    24301 aagaaccgga aaataaaaaa agaacgaagc ttcaccttag tggaagcttc gttctttttt
    24361 attgctttta tcaattcaat tttagcgtct caccgggagt caggaaataa ttctccatgc
    24421 tcatcccatt cagcgacaag atcgtttcta ctggtacgcc agttcgttcg gctatttgat
    24481 ttggaccttc tccttccaaa actacggttg tcgcagcctg tgaagtcgcg ctgttggtat
    24541 tagtttcaga ggaatctgtt gtgtcttgtg ttgttcgagc ttgcatttca gaagcttcta
    24601 ccgactctgc tgtctcagaa tcagaagatg ttgtgtcttg tgtgcttgtt gactctgctt
    24661 gaagagaggc gctagtctgc aaataaatat cggaaatctt agcgacatga ttcgtataat
    24721 tagccgataa ttgattgatc aaagtatcta agtctacacc gtctttattt tctgctgcct
    24781 gatacgctgc acgtaatttt ctgatccctt cgtcggagat tgcttgaagc tcacgtgctt
    24841 tttggttagc aactgctgat aaaccagcta ttccctcttg atttccctga atttctgtct
    24901 ggtattcttc aatcaatgca ggcgtctttt cttcgatctt tttcgaataa tcatttacga
    24961 ttgcttgaag ttgctctggc aaaccatttg ctgttttttc ttcagaagtt tgtgtctctt
    25021 ttgttttagt tgatgactca cttgtttgtt tcgtctctgt tttctttgtc gttgtttccg
    25081 ttatggtcgt acttttcgct tctgacgaat tttttccatg aaaatataga cccgcaactc
    25141 ctcctgctat gatcaaaaac acggcaactg ctgccccaat catgatcatc cgcttacgag
    25201 ctgcctgttt ttgctgtctt cttcgggtca tccgaacatt ttctccccac tcattttcat
    25261 catacatttt tttcaccacc aatttttatg tataatagcg ttatcaaacg cttcatattc
    25321 ttattgtagc aaataattgt cgctgtgcac atttttatct ttgttattta taagaaaatt
    25381 tcaccagttg agatgattcc aagattgtcc gtcgatttct ccttgcagct gcattgttcg
    25441 ttcgtctatc tcagccatca gtttctttat ttcatataag agcgcacggt cttgatattc
    25501 tgatttttcg cttaattcat ctaattgttg gatcagccaa tcatttgtca taatgaagtt
    25561 cctttcattt cttgcagtgt aaaggtcaag tcttcgatct gctcttttgc ctcagtcagt
    25621 gcttctacga tatatgcttg tttttcagat tcttcttcct gcattttctg attttcctgt
    25681 actctttgaa taaaccagtc aatccgctga gtcagcgatg gtttgccttc caatgagaga
    25741 tactgctgat aagcaattag cttctcccaa cgtttttcct gggataaata cataaattgt
    25801 gctaaaacga atggcggaag ataggtcata tggaatttat ttgcgattcg ctgatacggc
    25861 ctcaaaaatt cggaaatcgt atattcttct tttcctccag cttggtattc tttttccgcc
    25921 acacctgttg tcaatacgat tcctagttct ttccctttta atacatgtgc tgctcgttcc
    25981 aagacattat cttgccaaat ttttaaatga gcaggagaac tataccagta aaacggaaac
    26041 tggaaaatga tccgatcatg ctgggccaag agctgtagtt cagtctctat atctattttt
    26101 ccgtctggat aacttgattc caaatgatgc caagtagctg gtaaaccagc agaagcttct
    26161 ttgaaaaatt gctgatttaa agattcttcg atcgttggat ggctaatgat gataagtgta
    26221 cgcatccctt ttcctccttt ttagtcttcg ctttacagtg tagcataacc gccacaagtt
    26281 tcttttgccg atgcttgtca ttttaaccag actaatcagg ttgtccaaga aactgcggtt
    26341 tgtttttcca actgtttttt caaggtatcc aaagcaatat tcttaaaatt ttcgtttttc
    26401 atacgtttac gatagttcgt agccgtttct ccggtactct ttttgaacat tcggctaaat
    26461 acgactagac tattgaaccc tacttgatag gcaatacttt ccacggattc atccgtcaga
    26521 gaaagaagaa acgcccctcg attgatccgt accccgatca aatattcctt gatactggta
    26581 cccatatgat ttttgaatac tcgcgacaaa tggctccgat tgaccgctaa atgctgggcg
    26641 atttcctcaa cagacaattt ttcagcataa tgtgcctcga tgtatttgac tgtttcaaca
    26701 gctaaagagg aatgaacctt ttgtacacca ccgctgacct ttccgccatc ttttaaaagg
    26761 actgataaaa aacggtaggt taatgctgtt aactgtaatt cattttccaa ggtttcttcc
    26821 gtatctttca tgcattctaa aataatgtcg atatattttt gtatgtcaga agagaccatc
    26881 gtgtattggt cttctctgaa aagcgactgc cgaatgattt cttctgcagc actcccacca
    26941 aatgaaagcc agcaatacat ccacggatct tctccatctg ataagtaaaa cgtcgagtct
    27001 cctggacgaa tcaagaacag atcacctttt ttcaattgga attgttgatt tttcacatga
    27061 taagatcctt tgccttccat cacgacatgc aggatgtaat cattgcgaat agcaggacca
    27121 aaagaatgaa acggcatcgt tttagagaaa ccacaaaaat caaaatacaa ttctttttct
    27181 acttgttgat gttccttttt taaaaaaatc gattgctcca cgtctatccc tccttactcc
    27241 tttagtataa tgcaacaaaa agttaaataa aagtcacaga aatatattaa aaaggaagcg
    27301 atttcttgtt aagtttaagt cattaaaaga tctttgttat tcgttgtaac cggtttactt
    27361 agtattatat attgaacagg agaggaatac agattatgaa aagcaaaatg atccaacgtg
    27421 tgtcctacgc tttaggcgct tttggacacg acacattcta tgttacatta agcacttatt
    27481 ttatggtatt cgtgacaagc cagatgttcg caggaacaga taaggcaacg aatgcaaaaa
    27541 tgattgctac agttacaagc ttagttgtag gtatccgctt agttgaaatt atttttgatc
    27601 ctttgattgg tggcatcatc gataatactc gtacaaaata cggtaaattc aaaccatggt
    27661 tagttatcgg cggcgttgtt agttccgtac tcttggtagc aatctttacc aatttctttg
    27721 gcttagctac tcataatacg actttattta tcatattatt tgcaatcgtt ttcatcttat
    27781 tggatagttt ttattcattt aaggatatcg ctttttggtc aatgattcca gctttgagta
    27841 cggaaacaaa ggaacgtgaa aaatttgcta cgtttgctcg tttcggctct tctctaggtg
    27901 caaatggtac tactctagtc gttgtcccta tcgttacttt ctttacctat cttgttactc
    27961 ataaacatat tgaaggcgct tcaggctggt tctggttcgg tgtgattgct gctgttattt
    28021 caggcggtac tgcgattatc actgccttgg gcacaaaaga agtcgatact gcgatccgtc
    28081 agcaaaatga aaaagctggg atcaaagatg tgttcaaagc agttgctcaa aatgatcagt
    28141 tgatgtggct tgcccttgct tatatcgctt atgcgattgc caatgcagca acaactgccg
    28201 tattattcta cttcttcaaa tatgtacttg gccaaccaac tgaattttgg atcgttggtc
    28261 tagttgctgc cttacttggt ttagtagctg ttccactatt cccaatgctt acacgcgtca
    28321 tctctcgtcg ctacgtttac cttggaggga tcgcaatgat gatttcttct tactttttct
    28381 ttacgatcgc aggttcaagt ttgccaatcg ttatcatcgg gctagtattg ttctacttcc
    28441 ctcaacaatt gattttctta tccgctttga tgacgatcac agactctgtt gaatatggtc
    28501 aatggaaaaa tggtatccgt aacgaagctg ttactctatc aattcgccct ttactagata
    28561 aaatcgctgg tgctttgtct aacgggatcg tagggtttgt cgctatcgct tctgggatga
    28621 ctggtaatgc tactgctgct gatatcactg ctcatggcgt aacgactttc aaagcttatg
    28681 cattctatgc tcctgctgct ttgatgattt tctccgctat tatttttgct tggaagatta
    28741 cattgacaga gaaaaaacat gcacaaatcg ttgagaaatt ggaaaacaaa ttggctccag
    28801 ctgatacaat ggaagattaa cttcacgatg cagtagttcc ttcccattaa gaaaggatct
    28861 tgaaaatgaa gattacagaa aaagattttg gccaaggtta tcacttgata accttggaga
    28921 acaaaaacaa gctagcactt tctatctctg atttaggtgc tagaatcgtc agtttaaaat
    28981 caaatgatcg agaattagtt ttgggatttg acaccgctga agaatatatc gaaaaagatc
    29041 cgtatatcgg tgcttctatc ggtcgcaccg cagggagaat cgaaaatggc cgcttttctc
    29101 tcaatggaaa aacttaccaa ttagccacag atccaaaaac tggacataat ctgcatggtg
    29161 gtgctcctgg atttgaatta aaaaaatggt cttatgtcat tttgaatggc gaaaatgaag
    29221 cttctgtcat ttttactact acaagtcccg acggagaaca tggttttcca ggtacaatgg
    29281 atgtggaaat ccgctatact ttgacaaaag ataatatctg gcgagtgacc tctcgcggca
    29341 ccagtgacca agatacttta ttcaatccga caaatcatgt ttacttcaac ttgacgggag
    29401 atgcttctca gtcaatcgat caacatgagc tttggctcaa tagcgaagct tatgctcctt
    29461 tgcgaacaga tagtattcct ataggggtaa aagaaaatgc tgcaggaagt gctttcgatt
    29521 ttcaaatacc taaaaaatta gcgtccgttt ttgctagtga tttggatcaa aaaaatcttg
    29581 ttgacggaat cgatcatcca tttttcttaa aagaaaccgg cttgggtaaa gaagccgctc
    29641 gcttgactag ccccgataaa agaatccaag tagatattgc tacagatgct tctagtgtgg
    29701 ttattttcac tgcaaatttc ggcacagaaa caccagaaat gcgcaaccga aaactggcac
    29761 atcacggtgg catcacattt gagactcaaa cagctccagg tgcagagcgc ttctcagcat
    29821 ttggttcgat tcatttaaaa gccggatcag tatttgaaac agtaacagaa ttcaaaatca
    29881 aaacaagaaa ggaataactc atcatggaaa tcaaaagaag cttaaacaca aaatttgccg
    29941 aaacattcgg acctaaaaaa actacacaat acttctcacc aggacgcatc aatttgattg
    30001 gtgaacacac agattacaat ggaggccatg tcttccccgc ttccatcact tatggaacgc
    30061 aaggtgtagc agcacctcga gacgataata aaatattagt ttattccaca aactttgacg
    30121 atatcggtgt cattagtttt actttagatg agctagaata tgataaaaaa gcaaactggg
    30181 caaattacgt aaaaggaatg gtccttaaat taaaagaagc tggacactca atcgatcatg
    30241 ggtttgagtt attagtggaa ggaacaatcc ctaatggtgc aggactttcc agtagcgctt
    30301 cattagaatt attagtaggt gttgtattag aagatctgtt tgatttaaaa acagatcgct
    30361 tggaactagt ccaaacaggt aaaaaagtag aaaatgaatt catcggtgtc aattcaggta
    30421 tcatggatca gtttgccatc ggcttcggtg agctagatca tgcaatctta ttagatacaa
    30481 atacgctgaa atacgaaatg gttccagtga aactagatgg ttatgcaatc gtcatcatga
    30541 atacgaataa gcggcgggaa ttggcagatt caaaatacaa tgaacgccgt agcgaatgcg
    30601 aagaagcctt ggcacgtttg caaaaaactt tgcctatctc ttctttagga gacttagatg
    30661 aagaagaatt tgaatcaact attgaccaaa tcggcgatga tacattgata cgcagagctc
    30721 gccatgccgt ttctgaaaat cagcggactt tgaaggcaaa agctgaatta gaagctggga
    30781 acttagaagc atttggtcaa ctgttaaatg attctcacca ctctcttcgc tatgattatg
    30841 aagtaactgg gatcgaatta gatacactag tagatgctgc tcaaaaacaa gaaggtgtct
    30901 taggtgcacg aatgacagga gctggatttg gtggctgtgc gattgctctt gtcaaagaag
    30961 aaaatatacc tgccttcaaa aacaatgtct atgatgaata catgaaagtt gtcggctatg
    31021 ctccggaatt ttatgttgca cacattggaa atgggacaac aaagatcgat ccagaagctg
    31081 ttcatgtttg ataaaaaaat caacgatctc agctaaaaac aaatagataa aaataacctc
    31141 tcagcaattt ccaaatgtga gccttttgct cgtatttgaa aattgctgtt ttttattgac
    31201 acggaaagag aacgtgtgtt ctaattgtgt tgaagaggtg attgttatgt gggcgaatca
    31261 aaatccggta tttgtttatg aaaaagaacc ttcaagagat attttatgta ttgactgcaa
    31321 atctttttac gcctccgtag agtgcgttga aaaggatctt gatcccctta caactaaatt
    31381 agtagtcatg tcctatccgt ccgattcgac agaaacacgg ggtagcggat taatcttggc
    31441 ttccagtcct gcagcaaaaa aagcttacgg catcaccaat atcagccgtg ccagagatct
    31501 tcccttcccc tatccaagtg atttatatat tgttgcccca aggatggcct attatatgga
    31561 aaagaacaga gaaatcaatt ctatctataa aaaatatgca gacgaagcga accatcatgt
    31621 ctattccgtc gatgaaagct tccttgatgt aacaaatagc atggcactct ttggtaaaac
    31681 aaatgcgtac gacatggcta aactgatcca taaagatgtg tttgagcaga ccggaatcta
    31741 cacgactata ggaattggcg ataacccgct acttgctaaa ctagccttag acaatggctc
    31801 aaaagacgga aagcaaatgt ttgctgaatg gcgttatgaa gatgtaccag aaaagttatg
    31861 gaaaatctca gatatcactg acttttgggg aatcggcaaa cgaacagcaa aacgtctgaa
    31921 acaaatgggt atacattctg tttatgattt agctcacgcg gattattatc acttgaaaag
    31981 caagctaggg gtcatcggta ctcagcttta tgcccatgct tggggcatcg atcgaagttt
    32041 tttgggacaa gaatatacac caaaatcaaa aagtatcgga aatagtcaaa tcctagatag
    32101 agattataca agacgagaag aaatcgagat cgttatcaaa gaaatggccg accaagtcgg
    32161 cactcgcttg agacgatctg cagcaaaaac acaagtagtc agcttatggg taggatactc
    32221 tttaggctat cttgatccaa atggcaagac cgggtttcac cagcaaatga aaatcgaagc
    32281 gacaaatgct agcagagatc ttgcagatgc tttattgacc attttccacc agcattacca
    32341 ctaccaggat attcggcgtg taggcgtgaa ctgttccgac ctcgtatacg cgacaggttt
    32401 acagcttaac ctttttgaag atcctgaaaa gcagataaat gaagcgaaag ttgattttgt
    32461 tgtcgatacg attagaaaaa aatacggctt tcaatcaatc atccatgcgc atagtctctt
    32521 gcaaggagga cgcgccatcg cacgtagttc tctcgttggc gggcatgcag gtggtatgag
    32581 tggattagaa ggtggaaaag atgagacgga cagcaaaaaa attcattgac tataacgagt
    32641 atcgagatcg gccatttgga ttaaaatggg ggactgcttt tgcgatggaa gaactagtca
    32701 acggtatcaa aaaaatgaag aagaagcacg caaacagcct cttcctaaac aacaaatgac
    32761 attgcaagaa atcgatagta tactgacaga agccttttta tctcgtaaag cagtaatcat
    32821 tcagctcaat ttaaaagatg aatttggaag actcttggat gaggtcgaag ggcacttcat
    32881 tggtgaagcc tatgaagatt actttatgat tgaccatcag ccgatattgt gggaagacgt
    32941 acggtatatt gagttaaagg aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga
    33001 agtgatcaac ttcaaaaaat aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg
    33061 gaattttggc ttatttttcg aagaagcaac ttctggaaca acgtatatta gatatcagag
    33121 gttgtgaaaa agtcgcttag ttttgagtaa caagccaagg cgctgatttc acaacctctg
    33181 tctatctatc catctattcg gtaagattca tgcatgacgt cttctttatt tagtatgtag
    33241 agcccttttt cttctgacca ttcaacataa atcagctgtt ttacatctgc atagccttct
    33301 ttcttcaagt tctccataag ccatgcttca gtcaattgat tggactgaag cactttatgc
    33361 tgaatttgcc cttcatctac aagcaggatc gataaagctt tgtcttcatc tgattttttc
    33421 aatacactca tttgaccggc attctctagg ataacatgtt tggcattttc aagaaaaaag
    33481 cattcttgct gtcttaagat tgctcgcaac tgctccatct cgatatggtt atttgtcaac
    33541 tctgtcatgt tgatgacgcc gtctttgatt agaacagatg gctcgccttt gacccaacga
    33601 ttgacctttt catttttttg gacgatacgt tcgataccat aaatcattac tgcccaaagc
    33661 gcaatggcaa ataggacatg ccctacatgg acattgtcat cataggttga ttcttctaag
    33721 atcccaccta aaacaagcgt atagactaaa tcaaacggtg tgatttctga catggatttc
    33781 ttcccaagca aacgtaccac taataataaa ccgatcaacc cgaacaccag tttcattgta
    33841 attgaaaaaa tcattttttc tccccctaac tatctgttaa ttaaattata tatgctttct
    33901 cacgaacacg catggaatat acaaggaaga aatgtatcct tttactaaaa aaaggactgt
    33961 gacactgtag tgacccccaa aagttagact tttttggagt ggagaaatcc actccttttt
    34021 tttatgctct aaaatgatat ttttacacaa ccgttacggt tgttttacga aattggacga
    34081 gactttgcca gtttaatttc tgttttatcc gttcattatt gtagtaatag atatagctat
    34141 cgatggctga ttttaattcc tcgaaacagt tataaacttt cccgtgaaaa atctcttgtt
    34201 ttaataagcc aaaaaaattc tccataagtg aattatctaa gcagttccct ttccgagaca
    34261 tgctttgaaa gattttgtac tccttcaatt tgtttctata agctttcatt tgatacgccc
    34321 aaccttgatc tgagtgaaac gtacgacgat agggacaatc gttagtggtc tggatagctt
    34381 cctccaaacc ttccattacc gctaatgcat tgggcttttc agagatacga taggataaaa
    34441 tctcactatt gaacatatcc ataaaaggat ctagatacaa tttcttttga cggataatgc
    34501 ctttggcatc cacttcatgg tatttaaatt cagttgtatc cgtcgtgatt ttctgatggc
    34561 agatactggt gtagaaacga cgatggat
//
LOCUS       DAZKOR010000020        33664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000020 DAZKOR010000000
VERSION     DAZKOR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.020"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1078)
                     /locus_tag="ACG8ZM_000911"
     CDS             complement(<1..1078)
                     /locus_tag="ACG8ZM_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0491889.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSR"
     gene            complement(1097..2137)
                     /locus_tag="ACG8ZM_000912"
     CDS             complement(1097..2137)
                     /locus_tag="ACG8ZM_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0491890.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            complement(2153..3544)
                     /locus_tag="ACG8ZM_000913"
     CDS             complement(2153..3544)
                     /locus_tag="ACG8ZM_000913"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0491891.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            3922..4905
                     /locus_tag="ACG8ZM_000914"
     CDS             3922..4905
                     /locus_tag="ACG8ZM_000914"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0491892.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            complement(5273..7411)
                     /locus_tag="ACG8ZM_000915"
     CDS             complement(5273..7411)
                     /locus_tag="ACG8ZM_000915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0491893.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(7450..9036)
                     /locus_tag="ACG8ZM_000916"
     CDS             complement(7450..9036)
                     /locus_tag="ACG8ZM_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0491894.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(9026..10246)
                     /locus_tag="ACG8ZM_000917"
     CDS             complement(9026..10246)
                     /locus_tag="ACG8ZM_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491895.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(10258..11064)
                     /locus_tag="ACG8ZM_000918"
     CDS             complement(10258..11064)
                     /locus_tag="ACG8ZM_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0491896.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            complement(11274..12554)
                     /locus_tag="ACG8ZM_000919"
     CDS             complement(11274..12554)
                     /locus_tag="ACG8ZM_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0491897.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(12598..14631)
                     /locus_tag="ACG8ZM_000920"
     CDS             complement(12598..14631)
                     /locus_tag="ACG8ZM_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491898.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(14665..15516)
                     /gene="rfbD"
                     /locus_tag="ACG8ZM_000921"
     CDS             complement(14665..15516)
                     /gene="rfbD"
                     /locus_tag="ACG8ZM_000921"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0491899.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(15614..16642)
                     /gene="rfbB"
                     /locus_tag="ACG8ZM_000922"
     CDS             complement(15614..16642)
                     /gene="rfbB"
                     /locus_tag="ACG8ZM_000922"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0491900.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(16664..17236)
                     /gene="rfbC"
                     /locus_tag="ACG8ZM_000923"
     CDS             complement(16664..17236)
                     /gene="rfbC"
                     /locus_tag="ACG8ZM_000923"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0491901.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(17250..18116)
                     /gene="rfbA"
                     /locus_tag="ACG8ZM_000924"
     CDS             complement(17250..18116)
                     /gene="rfbA"
                     /locus_tag="ACG8ZM_000924"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0491902.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(18216..18923)
                     /locus_tag="ACG8ZM_000925"
     CDS             complement(18216..18923)
                     /locus_tag="ACG8ZM_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0491903.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            complement(18928..19755)
                     /locus_tag="ACG8ZM_000926"
     CDS             complement(18928..19755)
                     /locus_tag="ACG8ZM_000926"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0491904.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(19755..20555)
                     /locus_tag="ACG8ZM_000927"
     CDS             complement(19755..20555)
                     /locus_tag="ACG8ZM_000927"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0491905.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(20548..21687)
                     /locus_tag="ACG8ZM_000928"
     CDS             complement(20548..21687)
                     /locus_tag="ACG8ZM_000928"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0491906.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(21892..23085)
                     /locus_tag="ACG8ZM_000929"
     CDS             complement(21892..23085)
                     /locus_tag="ACG8ZM_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0491907.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(23164..23370)
                     /locus_tag="ACG8ZM_000930"
     CDS             complement(23164..23370)
                     /locus_tag="ACG8ZM_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0491908.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(23363..24505)
                     /locus_tag="ACG8ZM_000931"
     CDS             complement(23363..24505)
                     /locus_tag="ACG8ZM_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0491909.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            25017..25652
                     /locus_tag="ACG8ZM_000932"
     CDS             25017..25652
                     /locus_tag="ACG8ZM_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0491910.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            25788..25880
                     /locus_tag="ACG8ZM_000933"
                     /pseudo
     CDS             25788..25880
                     /locus_tag="ACG8ZM_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            25994..26629
                     /locus_tag="ACG8ZM_000934"
     CDS             25994..26629
                     /locus_tag="ACG8ZM_000934"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0491911.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            26736..27974
                     /gene="mef(H)"
                     /locus_tag="ACG8ZM_000935"
                     /gene_synonym="mefH"
     CDS             26736..27974
                     /gene="mef(H)"
                     /locus_tag="ACG8ZM_000935"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFQ0491912.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            28201..28620
                     /locus_tag="ACG8ZM_000936"
     CDS             28201..28620
                     /locus_tag="ACG8ZM_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0491913.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            28625..28864
                     /locus_tag="ACG8ZM_000937"
     CDS             28625..28864
                     /locus_tag="ACG8ZM_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0491914.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(29089..30012)
                     /locus_tag="ACG8ZM_000938"
     CDS             complement(29089..30012)
                     /locus_tag="ACG8ZM_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0491915.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(30044..30808)
                     /gene="map"
                     /locus_tag="ACG8ZM_000939"
     CDS             complement(30044..30808)
                     /gene="map"
                     /locus_tag="ACG8ZM_000939"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0491916.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            30966..31406
                     /locus_tag="ACG8ZM_000940"
     CDS             30966..31406
                     /locus_tag="ACG8ZM_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0491917.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(31584..32153)
                     /locus_tag="ACG8ZM_000941"
     CDS             complement(31584..32153)
                     /locus_tag="ACG8ZM_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0491918.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            32407..33639
                     /locus_tag="ACG8ZM_000942"
     CDS             32407..33639
                     /locus_tag="ACG8ZM_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0491919.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT        10330 a         6648 c         5798 g        10888 t
ORIGIN      
        1 ctctactata tatactctcc atttgttcag tagtgtacga aacatcataa ctctttagtt
       61 cttgtgttat tttattagca tacggctctt ttattacttt ttttgcccat tcttgtggtg
      121 tttcatctaa tgatacaaaa tccacattat ttgttaactt tgtatctttg gaaatagtat
      181 ctgctaagac taattttaat tttgctgctt gtgcttctaa tccaaccatt ggcagacctt
      241 cataaagaga aggcattaca aaaacatcca tcacagataa taactcggga atatcgtttc
      301 tttctcctaa aaattttact cttttctcta tatccaattc tactgctttg gctttcagtt
      361 tatttctaag tgacccttca cctactaaaa tcaagtaaga atcagaattc atttttagta
      421 tttctaaaaa aatctctaac aaataagatt gatttttttg tttctctaat cgtccaatgt
      481 ttcccacaac aaaagtactc attgagatat ccaattcttt tctcttatta accttagctt
      541 ctgaattttt actataaagc tctaaatcaa taccattatg aactatctca aaggggtggt
      601 tttgaaacag actttcacca gcttcttgac taacggcaag gtagtctgtt gcattttgta
      661 acaacaattt ggttaaaaat ttacgcaata cattcattac aatattttga gattttccat
      721 cttcagttgc atggctatga gcaattctct gtctgattcc attctttttg gctaaccata
      781 aattaattcc cccgccaaaa taaacctgat tgtgaactat agaaaaagat tcttttttta
      841 gtaacttatt cacttctatt aaatatttaa aaatattttt cttaggatta ggtacaacaa
      901 aaatacggcc tcctaatctt tcaatctctg aatgatagtc ttgaacatct gcataatctt
      961 cataaatcaa aaaatcaaat tgatagtttg tacgatcaat tttacgatat aaattcatca
     1021 tgaaagactc tgctccacct aaacccattc gtccctgaaa atgcaaaatt cttttcatta
     1081 tattttcctc cattaactaa tatactcgct tcaaagcagc cattaaccct ggcattttca
     1141 cttcaacgaa gcttccaact tttacagcag ataaaaatag tggagctggc atattgtaaa
     1201 gcaactttgt tatttttttt gctgaagaat ccgtcttagc aatttgcttg gcttctcgga
     1261 tcactggatc actcaatatc tgctttgctt cgtctttctt ctcttttgaa gttcttgcag
     1321 aggtactata aatagaaaaa ataactgaag ttgtcccttt taaaagaagc gccgccatac
     1381 cgtctttatt aatatcgtct gttacagtat ttgctaatga atagaattct ttaaatgttt
     1441 cgaacttgtt aggtcggtat tgattagtga tccccttccc accttcaaat tcataaatat
     1501 attctacttt tgggttcatg atcacttgat gacagtattc ataataatgt aagttaaata
     1561 ttcgatcctc acaactatta taagttttaa atagaatttg gttatttttc agaatatcgc
     1621 tacgatagca tttgttccat acaacataca tcaactgttc attcattaac tgatacatgt
     1681 cttttataaa ctcttcgtga gttagatagt gacattctgt aactaagttt tctttttcgg
     1741 aaacgattct tccctcgtcc attgtacgaa aaacaaacga actaacaaca agatccgctt
     1801 ttggactctt ttctagcaaa tgatcataat ctgacaagat cgtttttact ggcgtgtcat
     1861 ctgcatcgaa gaataagaga tattctcctt ttgactgtct tattccttcg tttcttgcta
     1921 atccgggccc ttgattggtc gtagtaatca attcaaaacg ttcatcattt tcaatcagtt
     1981 cttgaatgat ttcctgtgtt tgatctgttg atccatcatt tactaaaacg acttcgtact
     2041 cttcataatc caattctttt agaatatcaa tcgttcgacc aatagttgct tctgcattgt
     2101 atactggtac gattatgctg aatttcggat tgttcatttt tacgcctccc aactagcgaa
     2161 agattttttc attcggaaaa gcaatttctg ccaatgagat cacattttcg tcttcttcaa
     2221 gtccttgaga ggacatttta tcaaatagga tcttgatggt ttgcagcaag atctttaaat
     2281 caagcatcaa tgaatatttt ttgatataaa gtaaatcaaa acgtaattta ctgttgtaat
     2341 cagagacata ttttccatag acttgtgcat acccagtgat accagcacga acattatgcc
     2401 gtaagtagta ataagggttt tctgcattga actgatcgac gaagaaagga cgttcaggac
     2461 gtggtcctac gatcgacata tcgcctttca gcacattgaa gatttgtggt aattcatcaa
     2521 agcggacagc tcgaataaat ttacctacag gcgttactcg tgcatcattg ctcttagcta
     2581 aaactggtcc agattttgct tcggctgtgg cggtcatcga gcggaattta tagattgaaa
     2641 attccttttg attttttgtg atccgcactt gtttatagat gattggtccc ggtgatgtca
     2701 ctttgactaa aattgctgtg aataacataa atggtgaagc aataactaat aaaacaagtg
     2761 aaacgatgat atcaaacaaa cgtttgaata gttcatattc aggttggatt ctaaatccag
     2821 atgcttcaat aatactttca tcttcgaaat tcataatatt tggattgatc aatgtcaagt
     2881 tttcaaatgt tgtattcaaa aacaattttt tttcattttt agtaactaat tgatagattt
     2941 tgattttttc agtttcttcg atatgagaag caagatagac aatatcgatg tctttaatca
     3001 tatttttgat gtttgcataa tagttatcaa gaatgactgc ttcgacttta tgtttgttgt
     3061 ttttctttga accaaaattt tggattgctg gtgcgacatc cttttctaac ccaataatga
     3121 cgaccttttt atctcgactt acgcgtaaat aaagtttata tacgagaata cgatagatac
     3181 ttaagaaaag cgtaccgaga acaaatgaaa gtaagataac aagacgtgga aaagcaaacc
     3241 agcggcccgc aaaagtcact accatgatcc ctagtgtcat caatacctga ccgatgactg
     3301 tgacaaacac gatgtcactg atcattcgat tataaaaaac atatgcaccc aacaaaatgt
     3361 tcaacgcaat aaaaatcact gaaataataa ttgctgagtg ttggaaagtt tctaaatttc
     3421 gtaatgggat ctctccacga aacttcagta agaaactact atatattgct gcattgtata
     3481 caataacatc cattaatata atgaatatcc gtcgtgcagc actccattct ccatttttat
     3541 tcatatatac actcctaatc acaaaataat atctctatta tcttaccata gtcaccttta
     3601 aaaacctagt ttataagcaa attgtaatgg aatttaaaaa tgtctaataa aaagctttat
     3661 acacctctaa tcaagacaag gactctaaat tacaaatctc cagatgaatc gattttcact
     3721 aatgttggta aattaaagaa gtcgaagtat ttgctgcttt ttttatccac tcataaagtg
     3781 aacctttata tttttcagag aatccatctt cccaaagcgc tgtctagtcc ataagaatac
     3841 ctgtattacg tgaagaatcg aaaaaattga aatcgatttt taatgacatt atgcttatag
     3901 aagaaagtga ggtaatatct tatgaataaa caagagttgc atgattttat tggttttcga
     3961 caaccaaata tatgtcaaat tgtcgcatat aaggatgggg tagaagttta tacagatgaa
     4021 tggaatggtt ataaaaaaga cgatacttgt catatcatgt cagcaacgaa aagtattgta
     4081 tctttgctga ttggcattgc tttagagcaa ggattaatca aaagtatcga tgataaagta
     4141 ttagattatt ttccagagta taaagtgaaa cgcggggaga aaactattta cgctattaca
     4201 atcaagcact tgctaacaat gaaagcaccc tacaaatgta aaggcgatcc atggacaaaa
     4261 gtctgttcaa gtgaagattg gacaaaaaca tcactagatt tattgggagg aagaaaaggt
     4321 ataaccggcg aattcaacta tcaaactgtc tgtttacaca tattaactgg cttgttgtat
     4381 cagacaagta agctaacaac tgttgatttt gccaataagt atctatttga tcctataggg
     4441 ataccaaacc atgtcaatta ttatgctgaa acagctgaag aacataagca gtttacaatg
     4501 gataagtctc ctaaaaccaa tgtctggttt tgtgatccac taaatatcgg gacggcagga
     4561 tatggtttgt gtttatcggc aagtgatctg gcaaaaattg gtgtattatg cctaaataaa
     4621 gggatgtata aaggaaaaca aatcgtacca cccatgtgga taaacgagat gttcaaacct
     4681 agaaccagtg ctttagaaga cttacaaaat atgagatatg gctatttatg gtgggtatta
     4741 gattctgaga acgaaatata ttcagcaatt ggtaatagtg gaaatgttct ttatattgac
     4801 gctaagaaca atctggttat ctccatcact tcttatttta aacccatgat aattgataga
     4861 gtggcttttg tagaggaata ccttaaacca tttattttaa aatagcctat attaaaataa
     4921 aatatcgatt ctttgacagc tagggttcgt gaaactttgg ctatgatttt gtatctattg
     4981 gcgctctgag catgaataag agcgtgggac aaaagtaaga aacacttttg tctcattctt
     5041 taaaaacgaa taaacggtgg gagcagaagc aacttcttct taaataagcc gaaattcaca
     5101 aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc tcggagttaa acacttctgt
     5161 cccaacctcg acttattttt ttgagtcggc accttctgcc tttttttgac aaaaaactat
     5221 aaaaagccgt tatgtgcgag cgaatcacaa cataacagct ttttattata gtttaatctg
     5281 gtctcaacga aaagtcgccg attccttctt ttgttaaatt atcattgtaa aatggatcat
     5341 gcgcaatata tgctggccat ttatcttgca tttttttgat ttcaccagca aaacgtcttt
     5401 gtttttctgg agtatcttca taacctcttg atttagattc gaaatggtac aattctgctt
     5461 gatgtgcata gacattgtaa cgacccagtt cataaacttt caagcacaag tcaacatcgt
     5521 tgaatgctac ttctaatgtt tcatcaaacc cgttaacagc attgaagtca gctgccttga
     5581 ccatcataca agctgcagtg actgctaagt aattgacgtc aatgaccaat cggccgaaat
     5641 aaccacaatg tgttcgatca tagttattca atgcatgccc tgcaacacca ccgattccta
     5701 ataatactcc tgcatgctgc gttgtatcat ctggataaaa cagttttgct cctacacagc
     5761 cgattcgatc aaactgtgca taagaaacca tcaccgtcat ccaatctggc tcaatcactt
     5821 ctgtatcgtt attcaagaat aaaaagtatt tgccgtttgc tttttctgct gctaaattgt
     5881 taattcgaga ataattgaat ggaatgtcaa tcaattctac gataaagcgg tctttcagct
     5941 gatgcttgta ttctgcaaat aattcttgca tttttggatc tgtacttccg ttatccgcaa
     6001 taatgatttc ataatttgaa taagacgttt tttcgatgat cgaatccaca caagttttta
     6061 gatcttcata gccattcttc gttggaatga tcacactgac caaatcctct tgcaagacgt
     6121 cataagcaat ctcataaaat ccagaaattc tgccaggacg tacgcttcct ttgatgtttc
     6181 ttctagacag ggcatctgtc aatgctttca ctccggaatc atagatataa cttttggctt
     6241 cgccattact tgcagttgat cctgggatcg tccgccaatg atacaagact cggtcaatgt
     6301 ggtaaatatg atctgcaggg atttgttctg tcacacgaag gaccaaatca taatcttgag
     6361 aaccttcata gcctttacgg aaaccgccaa gttctttaac aatgcttgtt cgatatacgc
     6421 ctaagtgaga gatataatta tttcccatca atgtatcagg cgaccagtct gctttgaaat
     6481 gcggatcaaa gcgattgcca tctgcatcga ttttatcttc gtcactgtag atcacatcca
     6541 gttctggatg tacgtttagc acttttgcca cttcatataa tgcaaatggc ggtaattcat
     6601 catcattatc taagagtgct ataaattctc cttctgctat ttccaatgca gaattagttg
     6661 ccagactgat gtgtccattt tcttttcgga aaacgacttt gattctatca tcttttgctt
     6721 gataggattc cagacacttt cggatagctg gatcagtaga agcatcatca ctgatacata
     6781 gttcccaatg atcatacgtt tgatccagta ccgaatcaat gcatttttcg agccatttga
     6841 tttcaacgtt atagacaggc ataagtatgg aaatcaatgg acggtacgca aatccttgtg
     6901 cttctttcct ttgctctttc aaatctggtt tctcatgacg actgatccac tctgcatagt
     6961 cgatcgatcc tggcttcaat tccaatttga cccgacggat cgcttttttt acgccatgag
     7021 tccttgcata atttatccct tttttcagta atcttttttt tctttcccat gaagattcaa
     7081 tgccgtcatc ataagggtat ttaccgttta atttcacagc tatgccattt tgatgtctcg
     7141 ctgtttggaa gtctaagatg gcttttcctc tcattttccc tgataaacgt accttaaaac
     7201 cgctttgggt cttaacatca agattatata aatgattgat atccaatctt acgacatgtg
     7261 ttgcttcagc aatcacattt tctttttcta cctttatcgt agggatttct ttggtcactt
     7321 catcaattgc ccagcccaca ataagcaaat cgccagtggc ccgttcacga gtgatttgat
     7381 cgataaatac ggcaatttct tttttttgca cgattcgttt actccttatc tttataagtt
     7441 aatcattatt tacatttcaa tgatccatga ttggccattt tgagctactg cttccactaa
     7501 gaatgaacgt tcttgctcgc aaaggattcg taactcaaat cccgcttctg ttccttttgc
     7561 gagctgttct gcttgattga cttcttctcg tgaaacagga attgcttcga aaaatggcga
     7621 ttggtcagct gataacttat aggacaaggg ctggcgatcc gaattagcga ttccccatcc
     7681 gatgattttt aatatttttg tttctgcatc ccagattttc tcgtcaatac atatagaaag
     7741 ttctttttct tggatttttt tcgaagtttc tttcttacgt cgtcctagtc tatgttttat
     7801 tgtccattca tcactttcca acaatgtgca atagcgattt gtggttgttt ttaacgtatg
     7861 gtctagtctt gtattttcct caagaagatg atgattttct ctttctaatt cgtcattttt
     7921 ctcagaaagc tgctttgtga cttcagccat gggacgaatc gtttccaaca attctttcat
     7981 tgttggtgtc aattccccga taaagcgata gtgacaatgg atagtcactt ctttcccaga
     8041 aatatccgtt agaacgaact ctggtaattc tttcccatca aacaaataac aatcatttac
     8101 tgttttgact acagcattcg aatcgataaa tgttagtttt tctccggcag cttctgcgct
     8161 gaattcgata aagctaggtt gttgcgcgaa gcgaaacacc attctttctg ttgtttctga
     8221 tacggggaaa cttagtgtct cattttcccc tgtgaaatta ttgaatggta tttgttggat
     8281 tgtttcatct tgtttcgttt gctgggttac ttctaacact tttacataat tagaattttg
     8341 tggctctgca atggaacttt gtgctactgg atgtttcatc aatgagaaaa agtattggta
     8401 gacttcacca aatggtcgca ttttgaaatc atagcgtaca gcttccggca attcttcata
     8461 agttgatttt aattctgtat cacctacagc taagtacaaa taatcttcaa tattaatgaa
     8521 taatcccgct ttttcaaaaa ctttttggaa gccatcatga gtataaaaag aattatgggt
     8581 ttcatctagg agaccataag aagcccacgg caattggtta ttgaacaaat cgatcatgac
     8641 agaattatgc gccaagttag ggaatgtaat caggatctct ccttcttcat tcaaaaattc
     8701 tctgactttc ttcaatgttt ttccaggatc taccaaatgt tctaaaacat ccgcaaacaa
     8761 gacgtaatca aatgtctgtc cagcataata gtttgcccac tcaaaacttt cgatatcacc
     8821 ataaaatcca tcttgagcaa attcgctgac aaaatcgaac aattccttat ctaactcaac
     8881 aatgctcact tcacattgtt ttgctccgat taaatgtttc gttaatcggc cgtttccggg
     8941 accaaactct aaaactttac ttcctggttt gatctgtgcg gcaatttttc ccacactgct
     9001 ttgttcatct aggtccattt caaaatcata tttcattgct atgagtccct tcttcccatt
     9061 tatggtctaa gaccacgatg ccttctccga tataagaacc gctgacaaac aagttcatgt
     9121 actttgtctt atataccaaa ggaaccgttg cattttcttc aaagaccgca acgtcaaaat
     9181 aatattcacc agctaacaaa cctaactttt catactccag ataaaagata ttctctcctt
     9241 ttttccaagg aatcttgata ccatctagta gagtattaag tccgcataca taaaaattgt
     9301 cgactgttcg tagcgcaatt cctaaaaccg gatttttgat tgtatcatct tttacttcat
     9361 aagttacttt aacgtatagt ttttctcctt gttccagcat atccaccgga tttttgtctt
     9421 tatccaacaa ttccgctttc gtcacgtcga cgatatctgg agcggcgaac cgttctactt
     9481 ggtttttatt ccgatccact gttttaactg attttttctt taaaaagttt tcataattcg
     9541 tcgttacttc catcgtatcg ccatattcga cgacttctcc gtttttcaac caataggaac
     9601 gatcacaaaa acgacgaatc gaactgatat catgagaaac gaataaaatc gtctttcccg
     9661 agttcttgat ttctgtaaat ttttccatac attttagttg aaattcaaga tcgcctactg
     9721 ccaatgcttc atccacaatc aaaatatcgg gattcacatt gattgctaca gcaaaagcta
     9781 gacgaacgaa catcccgctt gaataagttt ttacaggttg ataaatatgg tctccgatgt
     9841 cagcaaattc aatgatatca tcgaccattt catcgacttc ttctcttgaa aatcctagaa
     9901 ccattccatt taaatagata ttttcatagc cagaatactc aggattgaat cctgatccta
     9961 gttctagcaa agctgagatt tttccttcga tttccatcgt gccggatgtt ggtgtcagta
    10021 ctcccgtaat gattttcaac attgtagatt ttcccgatcc gttttctccc acgaaaccga
    10081 tcatctcacc tttttttatt tctacattca cgtttttcaa cgcataaaat acatcatgat
    10141 aggatttctt aaaaggattg agggcttcac gaaatcgatc cgaaggtttt gcgtacatat
    10201 tataagattt tgtgatatct tttagtctaa ttgcatattc tgtcatagct attctcctta
    10261 cagcacatct gaaaaatgtg gtttcaatct acggaacact gcagagccga ttacgaacat
    10321 tactaacgtg aaaacccaga aatacaaagt atatgttcca tgctcccaga accaagcttc
    10381 acttaaaaat gactcacgat aaccttgaac aacatagtac agcggattta atttcaatat
    10441 gcttacaaga cctgaatcaa actgattagg gctccataat atcggtaatg tccacatgat
    10501 caactgcagc acgatcgtaa ttaattgcat gatatcaggt aaaaatggtt ggatcgatgc
    10561 cgtcagccaa gtcaatcctg tcaagaaagc acacatgcaa aataaataat aaatcagctg
    10621 taaagacatt gctgaaggat atataccatt tataagcgcg acagctatgc caatcagaat
    10681 gaagaataag tgggtgtaca gattggatag gattttcgtc gtgggcaaga tacgaatatt
    10741 gaatacgact ttctttacta aataagtata ttcccgaaaa acaaccgtag ttgatgcaaa
    10801 tgcatctgag aagaagaacc agggaatcat cccggtaact aaatatacga taaatgggta
    10861 ttttgcatcg atcatttgtg ctttcagtcc aacaccaaat accagccaat aaatcaatac
    10921 agtaaatagc ggattgataa acgcccataa gattcctaaa aacgatcctg catatcttga
    10981 tttgaagtca ttgatcgaaa actgtagcaa taattttctg ttctgataaa tgtccttcat
    11041 aaataaatac aaatctttaa acatttgtgt tcctccaagc gattttcagt gaattacagt
    11101 caagtgccat tttaccatat ttttggtacc gagctgcgtt cttatttctt tcttaatttt
    11161 ctatgataca agattttctc taacgtttct attttacaga agatgctaga caattgcaat
    11221 aaaccaaaaa aggctgcgac ggttgtcaca gcctctctcg tattttgtgc ctatcaatga
    11281 tagcgttgga tcctggaaga atgagcgcta tctggttgtt cctcataaga tgtatcatca
    11341 taagtcatat cgtcatatgc ctcttccata acatagaatt cctccgcaac ttctggttca
    11401 tcatatcgat gaacggccat agccaacagg atataaataa tcggaaccca tgggtatgaa
    11461 gtcaatgtat cctccataaa tgactgcatc atcaccgcaa aaaaggccca gataaaagtc
    11521 atctgcccgt tggataaatc tgctttcaac gttaagcgac caataaaaag gataagcagc
    11581 aaagcagcaa tcccaaatcc tatcaatcca tagatattca aaatagaatt ataactattg
    11641 tggtttccta aaaattggtt tcctcgttgg aagataaatg ttttcattcc tagtaaaatt
    11701 tgttcagatt tttctcctag cgtctgatag aatttatgcc aaatatcttc tcgaccggta
    11761 aacaaattga ctttttcaga gacagccgcc agataagaaa tcggcaaagc taaaagaatc
    11821 gtaaccgtgt aagcaataaa tggcgcacga atgacacgca tcaacgattt aggtaaaaga
    11881 tccaatatcg caaaaacaat caacgcgact aatgcacctc tagattggca gacccaaatc
    11941 ccgagaaaag ctgcaacaac tgctactgca ccgatgattc gtacatacca tcgttcaaaa
    12001 gaactggcaa atccactgat caataaagca agagtcatca aagaacttcc gattatattc
    12061 gtattgatcc aaatatcaga aagcgaattg cctggagccc agatgctttg tccttctggt
    12121 acttggatct ctgtaaatac acgatataac gtgattccga ggaagaccga aaagacaatc
    12181 agataaatac cgcgatccca ttttgtccac tcgatccatt taattactaa acaaaataaa
    12241 ataatcaaag gaaacagtaa gtctccaaat aaagactcat ttcttaattc ccgaaagtaa
    12301 gaaaagaaaa tgaaaagggc aagcgcaaca cctagataaa gaaaatcgac gccttttgct
    12361 ttataaacat ttaaaatgag caaaatagcc acactaaaag cgataagtaa tgaactattg
    12421 ttatatataa acaaagttgc tggaaaacct aaccctgctg tccaaacaaa aaagactagc
    12481 gtcagtaaca aaagcaagaa accgacactc attttgttga caaatttttg tacgattgct
    12541 tgaattatct ccactctcct ttctactaac atttcaggat tattctcagc tagcctatta
    12601 ctgtacgact agctgatttt cctgagtttg tgtttgtggt acatctggat aatccggata
    12661 ttctacttta aaaatcatat aaccgtcttt atccggtcca tatagctgcg tgaactgcgc
    12721 ttcatataat tggttgtaat cagttaatgg tcgtttactg atcacatagt cgatccctaa
    12781 tttttctgcg tcttcaaaat tcaacgtgac cgaaaacata tctggacgat ctaaaatgaa
    12841 ctttgtatct tccttagcga taaaagctct tgtatgagca taccgattat aatacttctc
    12901 gtattcttct ttcggatcga ttttcttcca tgcagctata tctggataaa agcgtactgt
    12961 attgaaggat ttcactccca atgttggggt gaagttatat aattcatctt cagacaacca
    13021 aactttacca ggatcttgtt gatcgatttt ttggatttct tttgccagtg ttttttcaaa
    13081 aatcgctcct gtaccttcta cgactggatt gacgaaaaag ccagatacga taactaaggc
    13141 ggcaaaagcg aggtaaaaca agcgtctcca gccaaataat gcaaaagcta aaatcactgt
    13201 tcccaacaca gcaactgcga tggtttcaaa atccgaaaaa taattatcca ttttcgaatg
    13261 cgtaatcgca tagaaataag accatgcatt tactgccaac aaaatgaatt tataccaaat
    13321 aggaataaca tcttctctcc aaatataagc gataaaccag atcgataata aacaagccgc
    13381 aaaactaaaa gtaaggatgg ctcttaatgg aggaacataa gacagcagag tgatttctgc
    13441 tagccatttt ggcaacccaa cggtaatcca aaagatagaa aacagacaaa aaatcatcaa
    13501 aatacgtccc agtaactttt gttcgctatc ttttttgcca aataacacaa atggacttgc
    13561 taggaataca gttggaaaaa agtgatagaa cagggccact tcagagttat tcgaatagtt
    13621 gatatcttca aaaggaattt tccaatttgt aaggaaagaa aaaaattcgc caatcgtcca
    13681 ctttcctcca gtacttaccc gttttcctgg atataaggta ttcaaagaag ctaacaatgc
    13741 gtcttttgac gttagccaga aatgaaccat gactccgcca ataaataaaa ctgcccctcc
    13801 aatcaacact acatccaaca gatcccatga aactttcttg ctatagtaaa taagcaggcc
    13861 aacaaaataa aaaatcagca agtacgccag catcacttga tgggctgggt aaatcactaa
    13921 aataaaccca ataccgaaaa taaccaccaa aagcatcatc aacgttcgta gccatttttt
    13981 atcatgattg tagaaataat gataaaaagc agtcatcagt cccaaagtaa agaaaatcaa
    14041 atcgcccaca tgttgcatga accaccactg gactgctgga gaaaatgtca gcgcaaatgc
    14101 accgattagc gatagagctt tttttctctt cgttaatatc atcagtactt cgaatccaag
    14161 caagaccatg acgactaatt tcatcgccca ataaaaggaa agtcctcgat ctcttccgag
    14221 gaaaaagaat ccccagttga atggttttcc gataaccgta atatccttta caggagaatt
    14281 atacgcaatg atcatgtttt gtccgtttaa tgaataatcc tcgttcacca acggaaagtc
    14341 ctcttcagct tgtgagaggt aaaaaggtgt ctgcaccagc cattcgtctg accgcacttc
    14401 ccgattctgt ccaaaaatca catcagtttt tttcccatcg tctctttgcg acacatattt
    14461 atcccaaaca ccaagtgaac ttccgtttaa attaaaagta acgatgacca cgatgactaa
    14521 aacgccaata agatagcgga gttttagaaa caggtcaagc accaaattcg aatctgcctg
    14581 gttcgctttt ggcttttctt ttcgtcgtgc acttcttgtt tgtttttcca tatcatgctc
    14641 caaattctat ttgataaaag gaaattactt attcattttt ttggcagctt ccagcatttg
    14701 cgcaagtgct tcttgccaag ttggtatgac aaatcctaac tcttctgtct ttttcaggct
    14761 catgactgaa tactgcggac gtgttgcttt ttgcggaaat tcttctgacg taacaggtgc
    14821 aacttctaca tctgtatctt tcaatatttc ttttgcgaat tcataccaag aacagctatt
    14881 ttcgttcgac aaatgataaa taccaaatgg tgctttttct tcgatgacat ataccataaa
    14941 ttctgccaaa gtacgtgtcc aagttggtcg cccaaactgg tcatttacta cagtcagttt
    15001 gtcgcgggtt tccgctaggc gttgcatcgt gaagacgaaa ttgtgtccat agatcccgaa
    15061 aacccaagaa gttcgtatga tataatagtc ttctaaaatc tcttctactg ctttttcgcc
    15121 taataatttc gtacgaccat attcatttag tggattcgtt tggtcgtcta cttcgtattc
    15181 tacatttttg ttttttccat cgaaaacata gtctgtactg atatagacga attgagcatt
    15241 cacagctttt gctgcaagtg ctacatttct tgtaccatca acattgatct tctcatctaa
    15301 ttcttttgct tcttcttctg ctttatctac tgctgtgtaa gctgcacaat ggaaaataac
    15361 atcaggtttt aattctgtga tgtaagccat tgttttctcg gcatctgtga tatccaagtc
    15421 ttttgcatct gttgaaacat attgcagatt tttttcatct aataaatggc gcaattctgt
    15481 acctagttgc ccatttcctc cagttaataa aaacatatga ttatccgctc cttgattttt
    15541 attttaaaaa agagacaggc gagcgcctgc ctcacatgtg tttttccatt aatacagtga
    15601 cattttctct gtattattgt ccatttttgg catatgctgc ttcaacggcc tctttttctt
    15661 ttttccacca gtcttcatgt tcttcgtacc atttgatcgt gtcagccaac ccatcacgga
    15721 agttcgtaaa ctcaggcttc catcctaact cttcccgtaa ttttgtcgaa tcgattgcat
    15781 aacgcaagtc gtgtcctgca cggtcgttta catggtcgta cgcatctttt ggttgaccca
    15841 tcatttctaa gatcaattcc atcacggtct tattatcttc ttcaccgtct gcaccgatca
    15901 gataggtttc gccaattctg cctttcgtca aaatcagcca cactgctgaa gaatgatcat
    15961 tcgtatggat ccagtcacga acattctttc cttcgccata taattttgga cgaataccgc
    16021 ttaagatatt cgttacttgg cgaggaataa atttttcaat atgctgataa ggaccatagt
    16081 tgtttgaaca gtttgaaatg gttgcacgta agccaaaaga acgcacccat gcttttacta
    16141 acagatctga accagctttt gtagaagaat atggacttga aggattatat ggtgtttccg
    16201 cagtgaattt ttctccttcg ccttccccat gtcctggcag gtcttcacgt aatggaagat
    16261 cgccgtaaac ctcgtcagta gatacgtgat gatagcgtac atcatacttg cggcaagctt
    16321 ctaaaagtgt atacgtacca atgatattcg tttgtacaaa tgggaaagga tcttttaatg
    16381 aattatcgtt atgggattct gccgcgtaat gaacaactgc gtccgcttct tttactagac
    16441 ggtccactaa ttctgcatct gcaacatctc ctacgaccaa ttctacgcga tcgctaggaa
    16501 gtccagccaa gttctctttg tttcctgcat aagtcagttt atccaaaaca gtgacatgca
    16561 cttctggatg attgtttacc acgtaatgaa cgaaattaga gccgatgaat ccggcacctc
    16621 cggtaacaat gatgtttttc ataagaaaat ttctccttaa ttcttagatt tctccgtaaa
    16681 caaatggatt ttctgcttca aattctttta atgtcggatg acggtgatct ttttccgaca
    16741 taatcgctct tgatggatca attggccaat caatcgctaa atctggatca tcaaatgcga
    16801 tgcccccatc tgcttctgca ttatagtaat tatcacactt ataaaggaag ttcacatttg
    16861 gtgtcaaagt aacgaatcca tgggcatatc ctttaggtac aagcaactgt cgatgattat
    16921 gttcagataa gataaatccg tcccattgac cataagtcgg gctacctttg cggatatcca
    16981 ctactacatc taaaactgct ccagtgacta cccgaatcaa ttttgtctgt gcagcttctc
    17041 ctttttggaa atgaagcccc cgaaggacac cagcttcagc tgatagtgaa tggttgtctt
    17101 gaacaaagtc atatttcaat ccatgttctg caaattttgc ttttgaataa ctttctgtga
    17161 agaaaccgcg atgatcgcca aagacgtcca tttctacgat tttgacatct gttaattttg
    17221 tatctgttac tttcatctgt aaggactcct tattcagcgg ccaaattcaa taaatattga
    17281 ccgtattgat tttttttcaa tggctgtgca agggccagca attgttcctt atcgatgtag
    17341 cccatacggt aagcaatttc ttccaaacaa gccactttta agttctgacg tttttcaatt
    17401 gtttcgataa aggtagatgc ttcaagcaat gattcatgtg ttcctgtatc aagccaagca
    17461 aatccgcgtc ccataagttc tacggatagt tttcctttat ccaaatatgc tttattgaca
    17521 tccgtaatct ctagttctcc acgttctgaa ggtttgatac ctttagcgat agaaattacc
    17581 tcattgtcat aaaaataaag gccagttaca gcgtaatttg attttggctg agcaggcttt
    17641 tcttcaatcg acaaagcttg catattctca tcaaattcta ctacaccaaa gcgttctgga
    17701 tcattgacat gatagccaaa tactgtcgcg cctgtatctt ttgaagctgc tcgttgcaac
    17761 attttagaca gaccgccacc atagtaaata ttgtccccta ataccaagca gacactatca
    17821 tctccaataa attcttctcc aatgataaac gcttgtgcca atccatctgg actttcttgt
    17881 actgcatatt cgatctgcag tcctaaatca tttccgtttc caaataattc cttgaaacgt
    17941 ggtgtatctt gtggcgttga aataatcaga atttctttga ttcccgccaa catcagagta
    18001 gacattggat aataaatcat tggtttgtca taaatcggca tcaattgttt cgatgttgcc
    18061 tttgttaaag ggtacagacg tgttccgctg cccccagcaa gaatgattcc cttcatggat
    18121 atctgctctc ctttttgatt tcaaatttca ctttgttatt atctcatcaa ttgttatatt
    18181 tgtcatggaa atattaagga tttaaacatc ttttatcaac gtttaaacca ttttcgtttg
    18241 atcaacgccg taatcagatg aacacgccaa gaaatttttt gccgtaattt agaatgtgtt
    18301 tcaatctcac tcgcgtttaa atcatgtctt cggtacagag ttaacggttc tcttaggaac
    18361 ccagtcttct ttcctcccag caaaccaatc cacatatcat gcatcggcac ttctgacggg
    18421 atcggcaaaa tatcgggttt taaagaagac cggaaagcca taccagcacc aatatagtga
    18481 cttcttatca cattattaag ccatccttct ttggaatttc gaaacgaaaa atacgaatca
    18541 tggatctctt gcagctcgct gtccacaatg attaaatcag aaactactac tagctgatcc
    18601 ggattctgct caaaatagtc agtgatcttt ttcacttttg tctccaacca aacatcatct
    18661 tggtctgcca aaaagattag atctcctttc gtctgctgga tcgcataacc aaaattagcg
    18721 atcaatcctt gacgaggccc cgaaaaaagc tggattcgct tatctttttc tgcgtattca
    18781 gttaatatat cccatgtatc atctactgac ccatcatcag aaatgactac ttcatcttct
    18841 gctgaaagct gagataaaat actatctaac tgctctctta aatatctgcc tccattaaag
    18901 gtagctatac aaaccgaacg cacgaattca caccttccaa ttcttaacat atgagcgcaa
    18961 agtcatccgc cagatatgcc ggtttttgac ggtcaagaac tgtttagctg ttcttaacaa
    19021 taattgtcgt cggtgactgg ataaatgcct ttgatcatat tcatgataaa atcgattttc
    19081 tgcttgtaaa atagaacggt atcgattact tgaaactttt tcatagtcta aaaccgaaag
    19141 atcatgttcc attcgtacag gaaggatctc cacagttttt tccaactgat ccactttcca
    19201 aaacagccaa tgatccaaaa aatctaaaga aaaagcagga ttaaacccgc caatcttttt
    19261 tagaaatttg acagaccagg caacacctga attgattgcc atgattcgtt cagctgttgg
    19321 gccttcaact tctactgctt gagctgtacg attgatataa cgatctgaat ataggggaga
    19381 aacttgtcgg ctcccagaaa aaatcatcgg aactgcagca acacattcat ctgtaaaggt
    19441 tactttacgc aatagttcaa aataatcgtc gactagcctc gtatcctgat ccagagtgac
    19501 gagacaacgt gttccttcat ctgcttgaga caacgcaaaa ttataagcca ccgctaaacc
    19561 aggattttgc ggatcatgat gataaacgat attttctttt tcaaacagct cattttgctg
    19621 cctttgagga ctattatcgt aaataataag ctgaatttgc ttttcgctaa gtgctttgct
    19681 taatggtcca taagctggag attgactgaa tagttgctga taaagcacaa cgactactaa
    19741 tatttctggt ttcatcagaa aaaacgccac ccccacttat tgaaaaattt catcatagaa
    19801 gtcaaaaaca cgcgaaacag ttttgcattt cgatgcgcac ccttcccgta aaaatggata
    19861 actgcataaa agggtgtgta caataacttg taacctgctt tttcaaaact caaacacaag
    19921 tcgttgtctt caaagtacat gaagaaacgc tcatcaaacc cctttatctc tttaaattta
    19981 tcaatatcag ctaccataaa actacctgag atcatccgta cataactatt agcatcagta
    20041 ggcaatcctc ggcattcaaa cacagccagt cgtttgtcaa actttttttt gacccatttg
    20101 aaaggcacaa agcgcaaaac gtaatcaaaa acagccagct gctcacggat gaggtgctga
    20161 gttgttccat cttcattaag aatcttaggt gctaacatcg cacattccgg atgttctttc
    20221 aataacaaga ccaatgctgt aactgtctct tcatccacta aaatatctgg gttacaaata
    20281 atgccatagc gagaatcgct agctatcaga ttgtgattat gtccataacc aaatccggaa
    20341 ttttcatcat gaaaatagat atcgatgtag ggatatttcc tatactctgt tagtcttttt
    20401 ttgtactcgg gaagtgaatt gttgtcaaaa attcggattt ttactgctgt ttcatctttt
    20461 acagttgaaa taaaattatc tagtgtccga aaaattttat cactgtttgc cgtcacaata
    20521 ctaataagga tcggctggtt taatttgtta ttcatctata tgacggttcc atttcttcaa
    20581 atatttctgt tttacttgcg tacggtactc tttattccct aaaaatcgca atgtttttaa
    20641 aataaactga tgtttctcat tgatcagacc aatcaattca aataataaaa ttgcagcaaa
    20701 caacatggca ataatcaata tgatcgttcc gattcttcca gtataattca tgaccatagc
    20761 tgtgaaagaa aacatcaaag ccatcgcata aatcgtcagg acagcacctt tatgagtaaa
    20821 gcctaaactg attagtcggt gatgaagatg ttttttatct gctgtagaaa aagaaacacg
    20881 gtttgctttt ctcctaatga tagcaaaaaa cgtgtctgtt accggcactc ccatgacgat
    20941 cagcaaagaa atcgatgaaa caaaagtcac gttcttcagc ccttgtagtg aaaggacggc
    21001 catcataaac cctaggaata gagctcctgt atcgcctaaa taaatctttg ccggataaaa
    21061 attgtaagga aaaaaaccga taatacttgc tacaagacag aaaataacaa ctggaatata
    21121 cactgtagaa gcatggagga aaaaatatcc aatgattcct atcgtaatta aaccgatcaa
    21181 tgaaatccct gccgccagcc catccaaacc gtcaatcaga ttgaccgcat tcgtgatacc
    21241 gaagatccaa aaaatcgtta cgggaaaact aagccagcct agttggatat atccaataaa
    21301 aggaagcgtg aaggaatcaa tacgtatatc tgctacaaaa taaataataa gagaagcaat
    21361 caagatccct atactttttt gtttaggtgt cagctcatat ttatcatcga gcactcctgt
    21421 gactatgatt actaaagaag caatcaagat cggtacaata tacgattgag ggataatttt
    21481 acggaataag aataaagtcg atataacaaa tgaaataaag ataggcagtc cgcccgctga
    21541 aggcattgga actttattta ttcttctttc atttggtttg tcgaccatac ctgtttgtac
    21601 tgaaatgatt ttgaatacag gtgttaatat cagtgataat aaaattgtca gaaaaaatct
    21661 tataaccata ctaaaagaaa caagcatatg ggcacctact ttaattaaga gtcataatta
    21721 ttataaatta tatatgtctt tttgactatc tttttatatc ttctcaaatg tccgcaaagc
    21781 cttattttat cggctctacg ggcattttgc ttttgtggta aacctcgcat atctaggtct
    21841 atcttcttat attttccctg tcaatcgtgg ttaaaatcgt ggtaaatact tctatgcgat
    21901 tagacgctga acctctgatt ttgcggtatc tatggacgca tgagcgtacc agttcattgt
    21961 gatactgatg tttgaatgcc ccatgatata ctgtaaatct ttcgggttca tgttcttgct
    22021 tgccagcctt gtgcagaatg tatggcgtag cgtatgcggt gtaatatgtg gcaagggatt
    22081 gtccttgtgg tgcttgttgt atttctttac catacggata aataaggcgt tataatcaat
    22141 ggcaactttt ggattacctt tcggattgat aaacacaaaa ccgctgtgtc cgtctacctc
    22201 tatcgacttt atctttggtc ggctgttgat aacccgttga aatgcctgta tcgtttcttt
    22261 gcttaatggc acttgcctta tcccgctttt cgtcttaggc gtttcaatat aatagccctg
    22321 ttccttactc tttagtaact ggtggtcgat aatcacaact tcattcttga aatcaatgtc
    22381 ggctactgtc agtccgcaca gttccgagat acgaagtcct gtctttaaca gtatcagcac
    22441 atcatcataa tgcttgtgat acacgttgtc cgtcttaata aatgacaata aggcttgctc
    22501 ctgttcctct gtcaatgcga ctttctcttt ggtatcattt tctatgactt cacttagctt
    22561 gaaatcaaaa gggtttttcc tcacataatc atcttgtatg gctatgtaga atgacgcttt
    22621 taacgagcgt ttatggttgt taatggtatt gtaggaaaag cctttgtctt tcatgcgtaa
    22681 cgcccattct ttagcgtcag agggttttat cgtatcaatg ctcctagcac ctaacttgtc
    22741 ctcttttaat aaccgcatga gttgttcccg ttgtttctgt gtgcttttct tcacatttgt
    22801 cctctgtgcg ttctgtttgg catagagttg gcaaagcgtc attttcttgc ctgtgctgtc
    22861 gataccgtcc tcaatatccc gtcttatctg ctgttccagt tctcgaagtg agggtttttc
    22921 ccgttttccc tttggtgtcg ggtctgtggg tgtcaatctc caagcataca catattttgt
    22981 gtttcccaat gcgtctacat atttatataa gtattttccg tctgttcgtt ggctctctcc
    23041 agtatgcagg atacgacctt tgttatcccg tcttttttct ttcatggtgt ctgctccttt
    23101 ccttgttgga aagagccttg atatgacttg tgttcatcat aacacataca aggctcattt
    23161 gcattagatt gcgtccaatt tatcaataac cttttcaaac tgtcggcgtt taatctgtat
    23221 gcggttgcca ttcataatga gccagccagc gtccttgttt tcctctgcca atcgtcttaa
    23281 cttgttttct ccaatgcgga aatactttga cgcttcttca atggtaaggg tgtatttttc
    23341 ccatacggga atatcctgtg tattactcat aaaacacccc ctcactttcg attacgtctg
    23401 atatgtccgt acacatctgt tccacaagca tttcctgttt ttcagaaagg tgggtacatt
    23461 catcaatcat tttcatgtag tttgaacctg tcagcctgtc gatttcctct atcatcacga
    23521 ttgacggcat gacctgtctt tccagccatt gcagagtgcg ttccatattg acaggtacag
    23581 ggttcatttc aaaaggtaaa ctgtcatggg aaatgaatat ctcccacaat ggatagtcag
    23641 gaaattccac aaaatcggta atgagataaa acaccagccc atgagggtta tacgtcaata
    23701 acagttcttc aactgcctgt accgcctttt tgtcacgcag acgaatttca aaacggttga
    23761 taatatcggt atgtttcttt ttcgttgcct gttccttttg tttttcatac aggcagaaat
    23821 attttgtgct tgcctttgag ccgatataaa gggtacttgc taaattacgg ttttttccgc
    23881 ttaattttcc accttgtaca ttttcatagt ttttacagcg acattccgca ccaccatttc
    23941 tgtatttttc agatagcaca ggaatatcca gcagaccgca catatcattg attgccaagt
    24001 caaaacgtcg gataacaccg ccacagtcaa gacagcggtt taagaatgaa taccagtccc
    24061 tgttctgtgc ctgtaagaca tattccatgt tgcgtgagcc tgcccctttt aattctaaaa
    24121 atacgcccat gtgttcatca tcagaagcca tgaccttgat ttccccgtaa gcataatgct
    24181 ccttatagcc ataataccca tagtcataat ggataaaata atctgctttc atgccaagca
    24241 cctttcggat aacttccaat gcgtcagtcg ttggaaaacg gacagacaga taatcaacga
    24301 gcagataaaa aggcttatcg cagatatagt aattcagaca acgcaggata atgttctgtg
    24361 tttccttgct ggcgttagct tttccgttct caaagcggtt gatactatat cttgatacat
    24421 gggtaagact tgcaagttct ccttgtgaaa gaccgctggc aagacgtttg ttcttcattt
    24481 caagtgcaaa ttctaaattg ttcattgtat tcctccaatc ccacaatagc cttgaaatac
    24541 ctgtaaacca cctttcaaaa agtgtgatat gtactatctt cttaaaacct ctcattacta
    24601 ctcacaggtt tgggggtgta tggtttttga cttgtctttt gtgaaatagt gcccccctgt
    24661 tagataacgg gggcacagac aaggctcgca ggctcgccag ccgacaaagc atttcttccc
    24721 cttgacacaa ggtgtcatgg cggtaagcct gagaggcttc cgccaccgtg tcagaaatac
    24781 tttgtcagca agttctgttt tgccttttct aacaagttcc atttcaaccg atattcatat
    24841 ctacggattt aatcagatag gtatttcttt ttgaatgttt ttatatttca ttctattgtg
    24901 cttattcgat tacaagaaca tagtaattct tatatgctta tttgtcaata tctttttttg
    24961 cctttacaaa tcatattaag catgatagac ttataatagc gaagatataa cgagatatgg
    25021 agggttatgc aatgaaaata agccatttag gaaataatat acagaccata agaaaattta
    25081 ggggaatgaa acaacaggaa cttgcggaca aaatcggtat caatatgcag agcctttcca
    25141 agattgaaag agggttgaac tatcctgcct atgaaacgct ggaaaagata atggaagtgc
    25201 tggacgttac gcccaatgaa ttgttgtcgg gagaatggaa gtatgtcaat caatctgaaa
    25261 aggaagtctg tcagttttta agagccgaag aacgtctaaa tgcagaactg aaacatggac
    25321 actatgataa cttctttgac agcgaggaag aatggctgga atatgagtta gaaaagttac
    25381 gggaatatat tacagactat atcaacggga aaagcattga agcgtccgac ctttacccaa
    25441 tcaaagaatt tatccagcat ttgaagtttc aaaaattatt agaccgctat gatgatttat
    25501 acagtatgga tatgtttggg gaaagcatag agggacataa gtataagaca ccttatcaag
    25561 tcgtgaaaat gataaacccg aactcaaaag aagatatgga actgttacga gaagtattaa
    25621 ggaataacaa ttttgatgat gaggacgagt aacttttttc ctcttgcgag aagatacagc
    25681 aaacaaaaag cccgatcaag agtgcaaagc accaccaatg gtggcaaagc tcttgacagg
    25741 gctttttctt ttctgttgtg ggcaatcaag aggaagaaag ggataaaatc tgatttgtgg
    25801 taaatcaggc aaaggcaacg caggtatcac agtatgaatt gacaccgcat aaggaaagca
    25861 actggaagat tgtagaataa atgaaaaagg cttgcattct gcatttttat aaaattgcaa
    25921 gtctttatat tgacaagcat aaaatactaa gatatacttt aatcagacca acggtttgta
    25981 ttggaggtaa gttatggcac gaaataaata tccagaagaa acagtaaaac tcattttaga
    26041 tgtttctaca aaaatgtttt gtgaaaaagg atatgataac acatcattgc aagatattat
    26101 caatgaaaca aaactttcta aaggtgctat ttatcatcat ttcgattcaa aggaagatat
    26161 tttaaaagca atttttcaaa gaattggaaa tgaaaatgca gtaatatttg ctaaaattag
    26221 agataacgat agtttaaacg gtttagaaaa gttaagagaa atatttaaga cagcggtatt
    26281 caattctaac caaagcgttt tattgacagt ttctcctcaa ctattaaaca atcctcgttt
    26341 tcttgctatg caaatagaac aaatgtatga aattgtagta cctgaatttg ttgaacccgt
    26401 tttagaacaa ggtatcaagg ataagtcttt ggagattgaa aattcacatg aaattgcaga
    26461 agttatcatg gtattaagta atgtatggct caacccatta gttgaaatga cggataaaga
    26521 gggtatggaa aaacgttgca atacattcaa tgccctttta aatggaatag gcatagacac
    26581 attattagac gaagaaacaa tttctgcatt tatacagttt tgtaaataac tgtatttgtt
    26641 atccctatac gatagactgc tctataaaag agcagtttat ttaaaccatt atataaaccg
    26701 acggtctgtt tgattaaata aaaaggagag attttatgca agcagaaaaa aaacttttta
    26761 atcgtgattt tatacttgtt gttataggac agataatttc tttatttgga aacgctatcc
    26821 tacgatttgc attaccgtac tatttgttaa aagaaactgg ttcatcagct ttgtttggta
    26881 ttgctacagc ctgctctttt ttacctatgg ttattttgtc gctggtaggt ggtattattg
    26941 ctgacaggac aaataaaaga aacattatgg tcatactaga ttttgtaaca gcaatcataa
    27001 ttttagtatt ttatttgtca ttgggaaaaa ttcctatcat acctttgttt attgttgctt
    27061 tgatgttact ttatggtatt tctggtgcat atcaaccagc ggtacaagca agtattcctc
    27121 tattggtatc atcagataaa cttaccgtcg gtaatgctat aataaatcaa gtaaatactt
    27181 tagcaaactt tatcggacca gctataagcg gagtagtttt tcaattttat ggtatcaatc
    27241 ccattttaat tgttagtatt atatgtttta ctttttcagc cattatggaa atttttatcc
    27301 atattccaca ccaaaaaaga cctcgtgaag taggtgtaat tgcagttgct aaaaacgatt
    27361 taaaagatag ttataccttt ttgaaaaagg agaaacccat ttttatatcc gtaatctttt
    27421 tggtttgcat tttcaatttg gttttaagtg ctgttttaat tgttggtctg cctatcttaa
    27481 tttctgaagt tttaaaaatg ccagatacac tatatggttt ttcacaatca gcattatcat
    27541 taggtggttt atgtggtggt attttaacag ctcttgttgc ttcaaagtta aaactaaaaa
    27601 acctttatac cttgttactt atttgttctg tttctgtttt acttatggga atttcgctat
    27661 ttttagaatt gcctgcaata gtttcctact gggtagttac agtgatgtgc tttgtggcta
    27721 tgggtgtatc tactatgttt gttgttcaaa tttacactct tgttcagaca caaacaccac
    27781 cgcaattagt aggaaagata atggctacgc tggtagcagt tgctatgtgc ggtcaaccta
    27841 ttggacaaat ggtttacggt attttattcg atgtatttat acaagaaaca tgggttgtta
    27901 tgtttgtagc ttctattgct tcaatcatga tttcaatata ttcaaaaaga atttttcaaa
    27961 agttagagat ataatacaag gggttaaata gtatgttagg ctaaaaacaa tatataaaag
    28021 aaaatttgaa atctgctaaa aggatagtgt tataaaagaa agagtaatat tgcagtattt
    28081 aaggctcgca caaatatatg ttttgtgtgg gcttttttct taccattttt ctgtatttct
    28141 aaagggtata aacggtattc cgtttatata tttcagcatt agcagaaagg gggtgagatt
    28201 atgaggacat cttctttcga gcatatcgtc agattacagt tcaattcttt aatgctgatt
    28261 gtcattaaga acaaagtgaa aagtcgaaac agacagcttg caagacattc taaacgtgaa
    28321 gtattgtttt gtgaaatagc cgaaacgaaa caagttgaac gtggaacgat tgatacctgt
    28381 ttttatgatt gtgtatcatt taaagtcttg cactttacaa tttatgtatc tgatgaaaca
    28441 cttggtacag ttttacatag actgtcagaa aaacagcgta gtgcgatttt attacgttat
    28501 tttcaaggaa tgaacgacag agaaatttca gaactgtacc atgtatcacg ttctgctatt
    28561 tgcagtagaa gaactagagg actaaggaag ttaaaaatgc ttttgagcga aaggaagtaa
    28621 agaaatgagt aaagattatg gctatccctc ttttgaattg atttgcaaag cgtcaagcgg
    28681 agatgaaatg gcagttaaag agattttgaa gttttatgac ggttatattt ctaaattatg
    28741 cttgcgtcct ttttaccact ctgaaagtgg taaaatcatt atgcaggttg atgaagaact
    28801 aaaaggagaa attcatacag acatgatgaa agcaatattg aaatttgaaa tcggagtgaa
    28861 gtaatcaact gtataaaaaa atcgggagat acacagtttc gtgctatctc ccttagtagt
    28921 tttacggtca tatcaaggct cattcaatcg tggtaaattc gtggtaagat gagtgttttt
    28981 ctatacttca aaatgcttga atgtatagtg tttatgcgga taattgaaaa ttagatataa
    29041 aatttcaaat aaattactga tataaattta caataagaac tcttgtttct actttttttt
    29101 atgagaaaat ttagttttca atttattgaa ccattttcgg cttggatctc gacgtttttt
    29161 gatttgatga cccgtcacgt attcttgaac aactgcattg atgattccgc ccaaaatgat
    29221 gatcgtagca gcaaaattaa gccaaagcat gagtacaata aaacttccta tgatctgata
    29281 accgctgatc ctcgttgcaa aatacctagc atagatacta aacacttgtg ataacagcat
    29341 ccagccgaca gtagcgaaga tcgtgcccgg aatgatcgtc ttcaacttca actgagcatt
    29401 agggacaatt aaataaatca agaacatgat gacaaataaa gctaccaatg tgattggcca
    29461 cttcaaagtt tggaactgat cgataaagtt cacgggtatt tgtacgattg gctgaatagc
    29521 atccaaaatc agctgtccca acccaagaac gatagttacc ccactaatag caatcatgaa
    29581 caagacaatg acaattagtg agaagaaacg gacgatcaaa aaattttttc tattttctac
    29641 gccatacgct ttattcatcg caatttgcaa agcattgata ctttgacttg cagaccataa
    29701 agccgctaat gcagaaacgg ataacaagcc tcctgaactt tgtgtcaaaa ggtctttgat
    29761 tgcaggaccg ataaactggt aaacggtctc aggaatcact tctcgtaaat aggtcagtac
    29821 agtctcttga tcgatgtgga gaaacggtaa aatattacca aaagcaataa tcaatggaaa
    29881 taaagacaag agtaaataat aagcaacgac gacagaggtc gtactcattt ctgaatcagt
    29941 gatgcgcttt tgagtcgtct caataaagcg catcagattt tcattttgtt ttattttgtt
    30001 ccaaactttc atgtacctct cccgtaccta ttgactggat agatcaataa gtgccttctt
    30061 ctccttgtga tgtcaaaatt cttggaccat ctttagtaat agccaatgta tgttcatatt
    30121 ggcaagaaag tccgccgtct cttgtatacg cagtccagcc gttaggatcc attttcattt
    30181 tccaagttcc ggtattaacc ataggttcga tcgtgataac catgccttct ttcaaccgca
    30241 acccttttcc agcttctccg taatgaggaa ctgctggttc ttcatgaatc gtaggaccaa
    30301 cgccatgacc aataaaatca cgaacgatcg ctagattttc gccttctaca taagtttgga
    30361 tagcatgacc gatgtcacca atccgattcc ctacttgtgc ttgctcaata ccgatgtaca
    30421 aagctttctt cgttacttcc atcaatcggt cgatctcagg tgtagattct ccaactacat
    30481 aggcccaaca agaatcagaa atgccgcctt tcaaatcgat acacatatcg acttttacca
    30541 aatcaccgtt tttcaaaggc tttttacgag ggaatccatg acaaatttca tcatttatac
    30601 tgatacaagt cgcatattca tacccttcaa agccaatttg cgcagcaatt ccgccatgac
    30661 tctcgatata gtttctgaca aatacctcaa tatcccaact tgtgattcct ggttttataa
    30721 aatcacgcag atttttatgt acatctgcta ataaagctcc agattctgcc atcatttcaa
    30781 tttcacgtgc tgattttaaa gtaatcatat tttcgtctcc tttttacaaa tccctctcat
    30841 tttaacatag cggcatacat ttagccaaat gacattcccc aggtgtctct aggttttatt
    30901 ttcttttcaa tgatgttttg gtataattca taattgttaa ataattataa acgaggaggc
    30961 acatcatggc tttagcaaaa atcgtatatg ctagcatgac aggaaacact gaagaaatcg
    31021 ccgatattgt tgcagaagca tttgaaaatc tggatatcga agtcgaaatc aacgaatgta
    31081 cccaagtaga tgcagatgaa ttcgaggagg cagatatttg cgtagtagct acttatacat
    31141 acgatgatgg cgatctgcct gatgagatcg tagactttta tgaagacttg caagaattgg
    31201 atctattggg aaaaatttac ggtgtctgcg gatctggtga tactttttac gatgaattct
    31261 gcaaatcggt ggatgatttc gatgctgctt ttgcaaaaac aggcgcatca aaaggagccg
    31321 aaaatgttaa agtggatctg aatgctgaag aagaagatat cgaaaattta gaagctttcg
    31381 ctaaagaact agtcgcaaaa atttaatata cttgcaataa aagagccatc cagatttatt
    31441 caatctgtat ggctctttta ttcattgcat tcatggaaga gttcttcggg agttaaaaat
    31501 gcatacgctg taagaaaaac gaacttttcc ttattttgac acatctcttg ttacctactg
    31561 atttgtggaa cttttgagta tattcaatcg atttcgattg gttctttgaa tttcaagata
    31621 gcatctatac tgtcttggat catcttttca atctgttcat cagttggatg gatgatgctt
    31681 gaagacatca aagctgcgat cccagtagct atgatccatg tcccattata caaagattcg
    31741 atctgctctt ctgaaagatt cacataagct ggatcgcgtt tcactgtatt cacaaagtaa
    31801 gtataagaga aattctgcat ctcttttcct ccaccatatt cttctaagta cagtgaacga
    31861 tacaacttct tctctcttct tgcaaaatgg atataattaa aagcaagatc gataatcgta
    31921 ttccctgtat gctcaacagt gaacacttca tattgtaaat gatcatagat ctgctcaaaa
    31981 agagcttgtc tcaagtcatc catattttta aactcaagat aaatcggttg cgtggaacaa
    32041 ttcattttgt tcgcgatgtt tctcgcagta aatcgtgtaa atccatcggt ggcagctact
    32101 tcataagcag ctgccaaaat ctgttctcta gtaatcgtct tttttcttgc catatttttt
    32161 aatctcctta atttttttat aacgcttcca ccattgatta atttcattgt aacatgtttt
    32221 tttgatatgc aaaattagtt tttgattttc ttcacaagct gaaatagcaa gtagtaacat
    32281 atatcatgaa acttaacctt aaaaaaataa cctccttttt ttatttaaag cctcattgtt
    32341 tgaatcataa ttaaatggta tcatgctaat agttaccaga aaaaatggtg aggaggaatt
    32401 tttattatgt tatctgattt taatgaactt ctaaaaaaat atgcacgctt aattgcagaa
    32461 actggcgtag ccacagaaaa aggacacaca gttgttttac agatcagcgt ggaacaggca
    32521 ccccttgctc gattgatcac acaagaagct tataaactag gagccgctga ggttatcgtt
    32581 caatggactg acgaccagat ccaaagagaa tttcttttac atgctgcaac ggatcgtatt
    32641 gagaatgtac cacaatctaa aatcgatcaa gcggatgaat ggcttgaaaa aggagcaagc
    32701 aggatcagcg ttgtttctgc cgatcccgat gcatttgctg gtgtagatag ccatcgagta
    32761 gcgtcttatc aatcagcgaa tggcaaagca ctaatgaatt tacgcaaggc aactcaagca
    32821 aataaagtca gttggactgt cgtggctgcg gctggtaaac aatgggcagc aaaagtcttc
    32881 ccagaactgc cagaagaaaa acaagttgag gcattatggg atcagatttt caaaacgact
    32941 cgtgtctatg aagaagaccc tgtcttagca tggaagaagc atgatgaaaa attagcaaaa
    33001 aaagcagaag aattgaatcg agaacaattt tctgcccttc actatactgc tcccggtaca
    33061 gatatcatta tcggattgcc taaaaaccat ctttgggaag gcgctggaag ctataatgct
    33121 cgtggagaaa aattcatggc aaatatgccg acggaagaag tgttcacagc acctgacagt
    33181 catcgagtag atggttacat ttcaagtaca aagccactga gttatgctgg caccatcatc
    33241 tcgggtatga aatttacttt taaagatgga aaagttgttg atttttctgc cgaacaagga
    33301 gaagatgtct tagcaaaact gcttgataca gatgaaggtg cgcgtcgttt aggagaagtc
    33361 gcacttgtac ctgacccttc acctatttct caatctggca tcatcttttt caatacatta
    33421 tttgatgaaa acgcatcgaa tcacttagca ctaggttctg cttatgcctt cagtgtgaaa
    33481 ggcggtacag aaatgtccga tgaagaatta gcagaagctg gattgaatag aagtcaaacc
    33541 cacgtagatt tcatggtagg atctgacaag atggatatcg acggaatccg tgaagatggc
    33601 tctacagtac cgattttccg aaatggcgat tgggcttgag tatactttcg cacacatgca
    33661 aaaa
//
LOCUS       DAZKOR010000021        33345 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000021 DAZKOR010000000
VERSION     DAZKOR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.021"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG8ZM_000943"
     CDS             <1..1152
                     /locus_tag="ACG8ZM_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFQ0491920.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8ZM_000944"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8ZM_000944"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0491921.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG8ZM_000945"
     CDS             2728..3003
                     /locus_tag="ACG8ZM_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0491922.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG8ZM_000946"
     CDS             3009..3776
                     /locus_tag="ACG8ZM_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0491923.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG8ZM_000947"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG8ZM_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0491924.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8ZM_000948"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8ZM_000948"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0491925.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG8ZM_000949"
     CDS             7920..8222
                     /locus_tag="ACG8ZM_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0491926.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG8ZM_000950"
     CDS             8225..9388
                     /locus_tag="ACG8ZM_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0491927.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG8ZM_000951"
     CDS             9414..12842
                     /locus_tag="ACG8ZM_000951"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0491928.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG8ZM_000952"
     CDS             12863..13987
                     /locus_tag="ACG8ZM_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0491929.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG8ZM_000953"
     CDS             13968..14414
                     /locus_tag="ACG8ZM_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0491930.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG8ZM_000954"
     CDS             14398..14736
                     /locus_tag="ACG8ZM_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0491931.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8ZM_000955"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8ZM_000955"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0491932.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8ZM_000956"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8ZM_000956"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0491933.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG8ZM_000957"
     CDS             15893..16936
                     /locus_tag="ACG8ZM_000957"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0491934.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACG8ZM_000958"
     CDS             17095..17823
                     /locus_tag="ACG8ZM_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0491935.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACG8ZM_000959"
     CDS             17939..18184
                     /locus_tag="ACG8ZM_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0491936.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACG8ZM_000960"
     CDS             18181..18399
                     /locus_tag="ACG8ZM_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0491937.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACG8ZM_000961"
     CDS             18535..19074
                     /locus_tag="ACG8ZM_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0491938.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACG8ZM_000962"
     CDS             19067..21340
                     /locus_tag="ACG8ZM_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0491939.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACG8ZM_000963"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACG8ZM_000963"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0491940.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8ZM_000964"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8ZM_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0491941.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACG8ZM_000965"
     CDS             complement(23167..23436)
                     /locus_tag="ACG8ZM_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491942.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACG8ZM_000966"
     CDS             complement(23698..23826)
                     /locus_tag="ACG8ZM_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491943.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACG8ZM_000967"
     CDS             complement(23906..24361)
                     /locus_tag="ACG8ZM_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0491944.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="ACG8ZM_000968"
     CDS             complement(24499..26427)
                     /locus_tag="ACG8ZM_000968"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0491945.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="ACG8ZM_000969"
     CDS             complement(26624..27457)
                     /locus_tag="ACG8ZM_000969"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0491946.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="ACG8ZM_000970"
     CDS             complement(27649..28353)
                     /locus_tag="ACG8ZM_000970"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0491947.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="ACG8ZM_000971"
     CDS             complement(28376..29002)
                     /locus_tag="ACG8ZM_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0491948.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="ACG8ZM_000972"
     CDS             29328..31703
                     /locus_tag="ACG8ZM_000972"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0491949.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="ACG8ZM_000973"
     CDS             complement(31727..32218)
                     /locus_tag="ACG8ZM_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0491950.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="ACG8ZM_000974"
     CDS             32405..33325
                     /locus_tag="ACG8ZM_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0491951.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT        11023 a         5845 c         6926 g         9551 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttaga
//
LOCUS       DAZKOR010000022        33215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000022 DAZKOR010000000
VERSION     DAZKOR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.022"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..228)
                     /gene="rpmB"
                     /locus_tag="ACG8ZM_000975"
     CDS             complement(40..228)
                     /gene="rpmB"
                     /locus_tag="ACG8ZM_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0491952.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            498..860
                     /locus_tag="ACG8ZM_000976"
     CDS             498..860
                     /locus_tag="ACG8ZM_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0491953.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            912..2594
                     /locus_tag="ACG8ZM_000977"
     CDS             912..2594
                     /locus_tag="ACG8ZM_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0491954.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2711..4747
                     /gene="recG"
                     /locus_tag="ACG8ZM_000978"
     CDS             2711..4747
                     /gene="recG"
                     /locus_tag="ACG8ZM_000978"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0491955.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4796..5797
                     /gene="plsX"
                     /locus_tag="ACG8ZM_000979"
     CDS             4796..5797
                     /gene="plsX"
                     /locus_tag="ACG8ZM_000979"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0491956.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5919..6161
                     /gene="acpP"
                     /locus_tag="ACG8ZM_000980"
     CDS             5919..6161
                     /gene="acpP"
                     /locus_tag="ACG8ZM_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0491957.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6429..7433
                     /locus_tag="ACG8ZM_000981"
     CDS             6429..7433
                     /locus_tag="ACG8ZM_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491958.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7434..8378
                     /locus_tag="ACG8ZM_000982"
     CDS             7434..8378
                     /locus_tag="ACG8ZM_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491959.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8378..9340
                     /gene="opp4B"
                     /locus_tag="ACG8ZM_000983"
     CDS             8378..9340
                     /gene="opp4B"
                     /locus_tag="ACG8ZM_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0491960.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9367..10281
                     /locus_tag="ACG8ZM_000984"
     CDS             9367..10281
                     /locus_tag="ACG8ZM_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0491961.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10307..12088
                     /locus_tag="ACG8ZM_000985"
     CDS             10307..12088
                     /locus_tag="ACG8ZM_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0491962.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12436..13122
                     /gene="rnc"
                     /locus_tag="ACG8ZM_000986"
     CDS             12436..13122
                     /gene="rnc"
                     /locus_tag="ACG8ZM_000986"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0491963.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13142..16723
                     /gene="smc"
                     /locus_tag="ACG8ZM_000987"
     CDS             13142..16723
                     /gene="smc"
                     /locus_tag="ACG8ZM_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0491964.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16732..17541
                     /locus_tag="ACG8ZM_000988"
     CDS             16732..17541
                     /locus_tag="ACG8ZM_000988"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0491965.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYGQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17552..18550
                     /gene="ftsY"
                     /locus_tag="ACG8ZM_000989"
     CDS             17552..18550
                     /gene="ftsY"
                     /locus_tag="ACG8ZM_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0491966.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18737..21004
                     /gene="gshAB"
                     /locus_tag="ACG8ZM_000990"
     CDS             18737..21004
                     /gene="gshAB"
                     /locus_tag="ACG8ZM_000990"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0491967.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21093..21545)
                     /locus_tag="ACG8ZM_000991"
     CDS             complement(21093..21545)
                     /locus_tag="ACG8ZM_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0491968.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21695..22645
                     /locus_tag="ACG8ZM_000992"
     CDS             21695..22645
                     /locus_tag="ACG8ZM_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0491969.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22638..23597
                     /locus_tag="ACG8ZM_000993"
     CDS             22638..23597
                     /locus_tag="ACG8ZM_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0491970.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23594..24349
                     /locus_tag="ACG8ZM_000994"
     CDS             23594..24349
                     /locus_tag="ACG8ZM_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0491971.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24372..25325
                     /locus_tag="ACG8ZM_000995"
     CDS             24372..25325
                     /locus_tag="ACG8ZM_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0491972.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25969..26265)
                     /locus_tag="ACG8ZM_000996"
     CDS             complement(25969..26265)
                     /locus_tag="ACG8ZM_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0491973.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26620..26976
                     /locus_tag="ACG8ZM_000997"
     CDS             26620..26976
                     /locus_tag="ACG8ZM_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0491974.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26986..27885
                     /locus_tag="ACG8ZM_000998"
     CDS             26986..27885
                     /locus_tag="ACG8ZM_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0491975.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28405..29133
                     /locus_tag="ACG8ZM_000999"
     CDS             28405..29133
                     /locus_tag="ACG8ZM_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0491976.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29278..31305
                     /locus_tag="ACG8ZM_001000"
     CDS             29278..31305
                     /locus_tag="ACG8ZM_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFQ0491977.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31413..32510
                     /locus_tag="ACG8ZM_001001"
     CDS             31413..32510
                     /locus_tag="ACG8ZM_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0491978.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32817..>33215
                     /locus_tag="ACG8ZM_001002"
     CDS             32817..>33215
                     /locus_tag="ACG8ZM_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0491979.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRL"
BASE COUNT        11110 a         5760 c         7119 g         9226 t
ORIGIN      
        1 aaaaccgttc actatctgaa cggtccaagt atcattcaat taaacgcgtt caacttttcc
       61 tgatttcaaa gcacgagttg acacccaaac ttttttaggt tttccgtcga ccataacgcg
      121 aactttttgt aagttaggtt taacagtacg ttttgttgag ttcatagcgt gtgaacggtt
      181 gtttccactt tttgtcttgc gacctgtaaa gtaacaaact tttgccatgg taatttcctc
      241 ctttgccttg atttggagca atagaataat tcagctcata ccagactaac atatcatata
      301 gaatggaaat ttgcaagtac attttcatag atacaattga caattgagaa gaataattgt
      361 aaattaaaca aggttatacc tgaaattttg caaggttttt gagtactttg ggtaactgcc
      421 ttttatttca agttgcgcta tgataaaata tgatagaaga aaaatgggag ttttttaccc
      481 gttaaggagg ctcattcatg gctgtaaaaa ttcaaacgtc agctggaacc attgaaatta
      541 ccaatgatgt aattgcaaca gttgttggtg gcgccgcgac agatgtgttc ggtgttgtag
      601 gaatggccag caaaaatcaa attaaagata atattactga gattttgcgt aaagaaaatt
      661 actcaagagg agtagttgtc cgccaagaag ataatggtat tgctgtagat gtttatacga
      721 ttatcagcta tggtacgaaa atctctgaag tttcccgcaa cgtccaagaa aaagtgaaat
      781 acaatctaga ggcattatta ggcgtaactg ccaattcagt taatgtcttt gtacaaggtg
      841 ttcgtgtact gccagactag gcaggagact ggctggtaac tcagcagtcg atctgaagga
      901 ggattatcta ggtgagtgta acagaaataa gtgcaagcca gttccaggaa atggtccagg
      961 caggtgcgaa tcgtctgcaa gtaaatgcag aatacgtcaa ttcattaaat gtattccctg
     1021 tgccagatgg cgatacggga acaaatatga atctatctat gactagtgga gctaaggcag
     1081 tagtagattc aacctcagaa aaagtaggag aattagcagc aatgctttca aaaggattgc
     1141 tgatgggtgc gcgcggaaat tccggcgtta ttttgtctca attgtttcgt ggattttcta
     1201 agcaaattcc agatgttgtc tcattaaatg cttccgatct tgctgcagcc ttcactcatg
     1261 gagttgaaac tgcgtataaa gcagtaatga aaccagtaga aggaacgatt ttgacagtag
     1321 ctagagaagc agcgaaagct ggagaaaaga aagcaaaaag tactgatgat gtcgttgaag
     1381 taatgacagc tgttgtaaaa ggggcaaagc gtgctttagc taaaactcca gacttgcttc
     1441 cagtcctaaa agaagtcggt gtcgtagaca gtggagggca aggactgctg tttgtttatg
     1501 agggcttttt aagtgcattg aatggagaat tccaagcgga tgaaacatat gaaccttctc
     1561 cagcagaaat ggatgatatg gtcaatgcag aacatcatcg cagtgtccaa ggtcaaatgg
     1621 cgacagaaga tatccatttt ggttactgta cagaaatcat ggtaaaaatc ggagaaggac
     1681 ctacagtcga tagtacattc gattacgaag aattccgtaa ctatttagat ggtataggag
     1741 attctttact tgtggtcaat gatgacgaga tcatcaaagt ccacgtgcat acagaaaacc
     1801 caggagaagt cttgaattat ggacaaaaat tcggttcatt agtcaaaatc aaagtcgaca
     1861 acatgcgctt gcagcatgag acgattttgg aacacgatga agaagtgaac gagtttgcga
     1921 aaaaagaaac accacgtatc ccgtatggta tcgtagcgat cgctgctggt aaaggcgtcc
     1981 aagagctgtt tgaaagttta ggcgctaatt atgttatcag tggtgggcaa acaatgaatc
     2041 caagtacgga agacattgtt aaagcgattc aagaagtaaa cgcagataaa gtgattgtct
     2101 tgccaaacaa taagaacatc ttcatggcag ctgatcaagc agcagaagta gcagatatcc
     2161 cagtagcagt tgttccttca agaacgattt cccaaggatt gacagctatg ctttcattca
     2221 atgatcaagc ttctttagaa gaaaacaaag aaacgatgac agaaatgttg tctagcgttg
     2281 tgagcggtca agtcactcac gcgatcagag atacctcaat tgacggtgta gagatcaaag
     2341 aaggagactt tttgggcatg atcgatggaa aaatcctgat ctctaaccca gatatcacag
     2401 caacagcgtt aagtacacta gaacaaatga tcaatgagga cacagagatc gtaacgatct
     2461 tggcaggtga tgaaggaaca gttgaacaag caaatgaatt tgctgagaaa ctaacagaaa
     2521 aacatcctga tttggaaatc gagatccacc aaggagatca gcctgtctat ccttatctat
     2581 tttctgctga ataatttctg tgtcagaata tctttctaac acagtattca ttcataaaag
     2641 gccttcggtt agtgatcagg tatgatcgct gaccaagtcc tttttttgtt aagggaggga
     2701 ggaaaaattt atgcgttctt tagatgatcc aatcactatg ctggcaggag tcggagaaaa
     2761 aagagcagct agtcttgcca gcttgggcat ccaaacgatt gaagacttac tgacttatta
     2821 tcctttccgc tacgaagata ttcaggaaag aaatctaaac gagatccaag atcaagaaaa
     2881 ggttactttg aaaggaatcg tagtctcgcc tcctgtgatg aatcgatttg gttataaaaa
     2941 aagccgattg caatttcgta tgatgcaaga acatgatgtg ttcaacgttt ctttttttaa
     3001 tcaaccttat ctaaaagaaa aagtcatttt atcggaggaa atcgccgtat acggtaaatg
     3061 ggatgcaaag cgtaaagcat tgaacgggat gaagatttta ggttctcaaa caatggatga
     3121 cttttcgccg atatatcacg tcaacaaatc aattcgtcaa acgacattag ttgatctaat
     3181 caaaaaaggg tttcaagaat acggcacatt gattgaagaa aatctgccaa acaaccttgt
     3241 ggagaaatat cgactgcttg accgagcttc tgctgtaaga agtatgcatt ttccaaaaga
     3301 ccacgaagaa aaccatcaag ccaaacgacg tgtcgttttt gaagagttct ttttatttca
     3361 aatgcgtctt caaggattga aaaaagcaga aaaagcagaa acgaatggtt tggagatcct
     3421 ttatgatgtc caaaaattga aagaatttac tcaaaagcta ccttttgaac tgacaaatgc
     3481 acaaaaacgt gtaacgaatg aaatctgtcg ggatcttcgc agtccgcagc atatgcagcg
     3541 acttttgcag ggagatgtag gtagcggaaa aacagtggtt gccgcgattg cgttatacgc
     3601 taccgttaca gcaggtttcc aaggtgcgtt gatggtgcct acagaaatct tggcacaaca
     3661 acatatggaa agcttgcaac agctgtttga tcccaatgaa gtaaccatcg cattgcttac
     3721 ggggtcaacg aaagcaaaag aacgtcgtga gctccttgaa aaactagagc aaggggaaat
     3781 taacatcgtg atcggcacac atgcattgat ccaagatgga gtcgaatttc agaatttagg
     3841 tctagttatt acggatgagc agcaccgttt tggcgtgaac cagcggaaag tacttcgtga
     3901 aaaaggctgg cgaccagatg tcttatttat gacagcgact ccgattccta gaacattagc
     3961 tattacagca tatggtgaga tggatgtatc tatcattgat gaacttccag ctggacgtat
     4021 cccgatcgaa acacgctggg taagaacacc tcaattggac agtgtactgg attggacgaa
     4081 aaaagaactg gcaagaggac atcaaatgta tgtcatctgt ccgctgattg aagaatcaga
     4141 agcattagat gtaaaaaatg cagtggaaat ctatgaaaaa cttcgtgaac tatttgcacc
     4201 aaaatatgaa gttggcttgt tacatggtaa aatgaagaat caggaaaaag aagtaatcat
     4261 ggaagccttc aaagacaacc agatgcagat attagtttcg actactgtaa ttgaagtagg
     4321 ggtaaacgtg ccgaacgcga ccgtcatgct gatcatggat gcggatcgat ttggattggc
     4381 acagctgcat caacttcgtg gacgtgttgg acgcggaagt gatgcttcct actgtatctt
     4441 agttgctaat cctaaaaatg aactaggtgt ggaacggatg aaaatcatga cagagaccac
     4501 caatggtttt gtactaagcg aaaaagatct ggaattacgt ggtcctggtg aagtatttgg
     4561 cttccgtcaa tctggtctgc ctcaatttgc gattgctgac ttggtgacgg atggaaatgt
     4621 tttggaggta gccagagaag aggctgcagc cctttggcag ataaaggatt ggcagatgct
     4681 tcctgagtat cagcctttag cgcaaagcct ccaaatagaa aatcaggata atcgttattt
     4741 cgattagcag actgtgctat actttatact gatgacggaa aaaggagtgg aaacaatgaa
     4801 aattgccgta gatgcaatgg gcggagatca cgcacctcaa gcaattgtag aaggcgtggc
     4861 tttagcgcag caagattttc cagaagtaga attcttactt tacggaaaag aagcagaaat
     4921 caagaaatac ttaacaaatg aaaaaaacat cacgattatc catacagacg aaaaaatcaa
     4981 tagtgatgat gagccagtaa aagcaattcg acgaaaaaag actgcatcaa tggtccttgc
     5041 tgcacaagca gtgaaaaacg gtgaagcaga tgctgtattt tcagcaggga atacaggcgc
     5101 gctactagca gcagggttgt tcatcgtagg acgtatcaaa aatatcgaac gccctggctt
     5161 gatgtctacc ttgccagtag tagggaaaga aggtgccggt ttcgatatgc tggatcttgg
     5221 cgctaacgca gaaaacaaac cagaacattt gttgcaatac ggtattttag gttcttttta
     5281 tgcacgtaaa gtccgaggta tcggacgtcc tcgtgtcgca ctcttaaaca acggaacaga
     5341 agcaacaaaa ggtagtgaag tgacgaaaaa agcctatgaa ctccttcaga atgaaacaag
     5401 cttgaatttt attggaaacg tagaagctcg tgagctactc aatggtgtgg ctgatgtagt
     5461 ggtcacagat ggatttacag gcaacgctgt tttgaaatct attgaaggaa cagcaatgaa
     5521 tatgatgaat ctattaaaat ctgctattct aaatgaaggg atcaaaggaa aaatgggcgc
     5581 tatgctttta aaagacggct tacgttcttt gaaagcagaa atggattatt ccaaacatgg
     5641 cggtgccgtt ttgtttggct tgaaagcacc agtcattaaa actcatggag caacaggccc
     5701 cgatgctgtt cgctatacga tccgccaaat ccacacaatg cttgagacag atgtagttgg
     5761 acaattagtc gaacaatttg aaaaaagcga agaatagtat ttggatatct gaagcaatcc
     5821 gttccattga aaaaaaggtg gacattcctg aaaaatgcca gtaaaattag ccatggtgta
     5881 ggtaattgac aagtgtatga ttcttggagg tgaaaaagtt gactcgcgaa gaagtattta
     5941 acaaagtagc gaaaattatt tcaaatcatt ttgagataga taccgataaa gtgacggatg
     6001 aattgaacat caaagatgat ttaaaagctg attcaattag tattatggaa ttcgttttag
     6061 agcttgaaga tgaattcggt acggaaattt cagatgaaga cgctgaacaa attgaaacag
     6121 tcggtggcgc agtagattat atcagcagcc atcttaaata atttcaaata aaagcaaaga
     6181 aggataaaaa gctgcataca gctttttatc cttctttttt taatcttttt gacacgtaaa
     6241 agtcattttt ctgataattc tggcgataaa cagaaattat gttgcaaagt aaagcgattt
     6301 tctatataat tattaataat tcattacaca aagataagaa taacctttgt tataaaacgc
     6361 tttattctgt gtaatgtcct tttttattaa gaaaaacttc catttaggaa gcagagaggg
     6421 gtattcgatt gtccgataac caattattag aagttcagaa cctacataca ggattccgac
     6481 tcaaagatga ctattacgat gcagtagatg acgtttcatt tactttagac aaaaacgaga
     6541 ttttagcaat cgttggggaa tctgggtgcg ggaaaagtac cttggctact acgattatgg
     6601 gattacttga tcctaataac acaaaaatta caggtgagat tttatataac gatctgaatt
     6661 taatcgacct gaacgaaacg ctctataata aaatccgcgg aaatgatatc ggaatgattt
     6721 tccaagatcc gttgtctgca ttaaatcctc tcatgagaat cgaagaccag atcaaagaag
     6781 gactttccta tcacacaaag atgaatgcag aacagcgtca agctcgtgca ctagaattgc
     6841 tagaacaagt agggattcct aatcctgcga gagttggacg ccaatatccc catgaactat
     6901 caggcggtat gagacaacgt gtcatcatcg caatcgcaat cgcctgcaaa cctccaatcg
     6961 tcattgcaga tgaaccgacg acagccctag atgtaaccat ccaagcacag attcttgatt
     7021 tactgaaaga ccttcaggaa gaaacaaaaa cagggatcat cttgatcact catgacttag
     7081 gagtcgtagc agaaatggct gatcgagtgg cagtgatgta tggcggacag ttcgttgaag
     7141 tagcaggtgt aaaagaactg tttgatcatc ctaagcatcc ttatacacgc tctttactga
     7201 actctatacc acaagaagga aatcatgatg cagaactcca tgtgatcgaa ggtgtcgttc
     7261 cttctctgaa aaacatgcct cgtaaaggtt gtcggtttgc accacgtatt ccatggatac
     7321 cagcaagcga tcatgaagaa gagcctatcc ttcatgaagt cgcaccgaac cacttggtac
     7381 gttgctcctg ctataagcat ttccacttta gagacgaaaa aggagaggtg tagatgacag
     7441 aattaatcac aatcaaagac ctgaaagttc attaccctat tcgcagtgga ttcttcaatc
     7501 gagtaacgga tcatgtatat gctgtagatg gtgtggattt tattatcgaa aaaggaaaga
     7561 catatggttt agtgggagag tctggctctg gaaaatctac tacgggaaaa gccgttgtgg
     7621 gattagaaaa ggtaacagcc ggacaaatca tgtatgaagg aaaagatgta acgaagcgaa
     7681 gcaaccgcaa aaaaatgggt tataacaagg atgtgcagat gatcttccaa gattcaatgt
     7741 ccagtttgaa tcctaaaaag cgagtgttgg atatcattgc tgaacccatc cgaaattttg
     7801 aacggttaag cgatcaagaa gaaaagaaaa aggtaaaagg actgctagat atcgttggga
     7861 tgccggaaga tgcgctatat aaatatcctc atgaattttc aggcggacaa cgtcaacgtc
     7921 taggagtagc ccgtgctgtt gctaccaatc caaaactgat cgtagccgat gaacctgtat
     7981 ctgctctgga tctatctgtt caagcgcaag tattgaattt catgaaacga atccagcaag
     8041 aatacggcct cagctattta ttcatttcac atgatttagg tgtagtaaaa cacatgtgcg
     8101 acaatatcgc aattatgtac aaaggtcgat ttgtagaaat cggtacaaga gaagacattt
     8161 ataatgaccc gcgacatatc tatacgaaac gtctgctttc agctattccg caaatcgatg
     8221 tagaaaatag ggaaagccac aaagaaaatc gacgaagagt agaacaagag tatatccaaa
     8281 accaaaaaga atactatgat gcgactggtc gagtctacga tttgcgtacg atcacaacta
     8341 cacacaaagt ggcgttgaaa gatggaggtg caagctaatg tggaaaacga ttctgcgacg
     8401 tgttttattg atgatcccgc aagttattat tttaagtgtt ctgatcttct tattagccca
     8461 agcgatgccg ggggatccat ttaccggttt gatcaatcca aatcaggatc cggcagtgat
     8521 cgaacagatg cgacaagcag ctggcttgaa tgacccatgg tatgtccagt acttccgttg
     8581 gatcggaaat gctttgcaag gcgattttgg tcaaagtttc ttatacaaac gctctgttgc
     8641 tgacttgatc ggcgaacgga tcatcaacac tttatatcta tcgatcttga cagttgtcct
     8701 tacttatttg attgctgtgc ctctggggat gcttgctggc cggtatcaaa attcaatctt
     8761 ggataagatt gtcgtaatct acaacttctt tagttttgct gttcctttat ttatcttcgg
     8821 cttgatcatg ctatttgttt ttggttatcg tttaggctgg ttcccaacaa gtggatcaca
     8881 aacactaggt ttgcaaggtg gatttatcac tcaatggata gaccgtttga aatatattct
     8941 gttaccatca attacccaag catttttggc gactgccgtc acaatccaat atctgcgaag
     9001 tgaagtaatc gattcgaaat cgctcgattt tgtcagaaca gcccgttcca aaggggtacc
     9061 gacgaataaa gtattcagtc gacatatttt ccgaaatgca gcgttgccga tggcttctca
     9121 aatggggtat gaaatcactt cgttgattgc tggttctgta gttatcgaaa agatttttgg
     9181 ttatccggga gtcggaaaac tatttattga ttccatcggc caacgggact ataccgtgat
     9241 cacatcctta gtattgattt taggaatcgc gacgctagtg gggacattac tttcagatat
     9301 catcatgagt atcgttgacc cgcgtattcg gattcaataa aaccaatcag aaaggagaat
     9361 ggatcgatga gtgaaaaaaa caatacagca gttcaagaaa gtattcctcc tatggggata
     9421 cgaatgattg ctcgagaatt taaaaaagat aaaattgcta ttttttcatt aggtttgcta
     9481 gtggttttat tattagtgat ctttatcggt gcgatgctga ccgatcaaga caaagtgatg
     9541 tatgttagta tcttcgacaa atacgctgca ccaggaacag tttcgacaca aggacaaaag
     9601 ttcattctgg gagcagatga aggcggacgt gatgtattag gacaattgat cataggtgct
     9661 agaaactctg ttacgatcgg atttgcgatc acgatcctta cttcgatcat cggggttgga
     9721 ttagggatca tctctggcta ctacggaggg atcattgaca acatcttgat gcggatcgtt
     9781 gattttatca tgattctacc gatcatgctg atcattatcg tcttcgtatc gattatttcc
     9841 agatacaatg tctggtcctt tatattcatc atgagtgcat tttattgggt agctaaagcc
     9901 cgattattcc gaagcaagac attatctgaa gcaagaagag attatgtcag tgcctcaaaa
     9961 acaatgggaa ccaatgattt taaaatcatg ttccgcgaaa tcatgccgaa tctaagttca
    10021 ttaatcatta ctaatttgac gatgaacttt gcggcaaaca tcggtatcga gacaactttg
    10081 actttcttag gtttcggctt gccggcaaat gtacctagtt taggtacatt gatcggttat
    10141 gcaagtaacg gagaagtctt agtgaatcgg caatggatct ggttacccgc atcaatcctt
    10201 attttagttt tgatgttgag tatcaattat gttggacaag cattcaagcg ttctgcagat
    10261 gcgcgccaac ggttaggcta gtatagaagc gaagggggaa ataggaatga agaaaacgat
    10321 ttttggattt gcagcgctga tgtctgctgt cgttcttgca gcatgcgggg gcggaggtac
    10381 agatacctct ggaaacaact ctggaggcaa cggcaataac aaagaggaat taacttttcc
    10441 aattgcaaca aaaaataatg atgatgcaat cgaagacggc gaattagaag ctgcggtcgc
    10501 aacggacagt cagtttaaag gattgttcca gtgggagttt taccaagatt catatgatgc
    10561 agcttttatg gctccttctc atgaaccgct atttgcaaac gatgacgatt tcagaatcac
    10621 agacgacgga gctgcaacgc ttgatctaga tgaagatacg aaaaaagcga caatcaaagt
    10681 aaaagacaac gtcaaatggt cagatggtga agatgtaacg gcggacgatg tgattttccc
    10741 ttacgaaatc attgggaata aagattacac tggtatccgt tatgacgaca ctttccgtaa
    10801 catcgtaggt atggaagaat atcatgatgg aagtgccgac acaatctccg gaatcaaaaa
    10861 aatagacgac aaaacagttg aaatcgaata taaagaagta aacccaagca tgcttcaatc
    10921 gggtggtggt atctggtcat atgcagcacc aaaacatacg ttagaaggtg tagcaatcaa
    10981 agatatggaa tcaagcgatc aagtacgtaa aaatccagtg acttttggcc cttattacat
    11041 gagtaatatc gttgcaggag aatcagtaga atacttgcca aacgagcact actggaatgg
    11101 tacgcctaaa ttgaaaaaga tcacgatgaa atctgtaccg atggcaagtg caactgaagc
    11161 attgaactct aaattatatg atatgatttt ccaaatgcca acagatactt acgataccta
    11221 taaagaaatt gctggctata caaacttagg ccgtgaacaa acttcttata cttatctagg
    11281 attcaagtta ggtaaatgga atgcagatac aaagagtgtg gaatacaatc cagattcaaa
    11341 aatggctgat aaatcattac gtcaagcaat gggctatgca ttagataata atgcagtagg
    11401 tgaacgtttc tatgcaggtt tgagaagcaa tgcgacttca ttgattcctc cagtattcca
    11461 gtcataccat gatgatgaga tgaaaggatt cacacaagat ctagacaaag cgaaaaaact
    11521 attagatgat gctggctaca aagatgtgga tgacgatggt ttaagagaag acaaagatgg
    11581 aaataaacta acaatcaact ttgcttcgat gtccggtggg gaaacagcgc aaccattggc
    11641 agattactat atccaacaat ggaaagaaat tggtttggat gtccaataca caacaggtcg
    11701 tctgatcgaa ttcaattctt tctatgataa attagaaaac gatgatcctg aaattgatgt
    11761 ttaccaagct gcttggaata caggaactga tccaaatcca agtggactat ggggaccaaa
    11821 tgctgcgttc aactacactc gttatgaatc agaagaaaat acaaaattga tcaacgatat
    11881 cagttcaagc aaagcatttg atgaagagta ccaaaaagaa gcatatcaaa aatggcaaca
    11941 atatgcattt gaagaagcat ttgcgattcc aacgttgttc cgtaatgaag tattgccagt
    12001 caacgaccgt gtcaaagact gggattggtc ttatgatgta ccaaacccat gggcaaatgt
    12061 ttctgttaca gcagattcac gttcataatc actaaaaatg accgaaaaag ggcgatgtcc
    12121 tttttcggtt ttttgatatt tttctgttta tttctctaat attcaggtca aggcgctgat
    12181 ttcatgactt ctccaataaa cggtgttcaa agtccagttt ctcggtatta agtcaaattt
    12241 atgcaaaaat atggaaagcc attttcacaa aattttttct tcatactcga aactaaacga
    12301 atttttcaca acttctcgtc aatcactagg attattaaag ctttacttac cttttgaccg
    12361 aaccatttca aatttgacaa aaaaccagta taataaggaa gtatcgaaaa aatatccata
    12421 aaaggaatgt ggaaaatgga caaccagctg acaaaagaac taaaagaaaa ctataacatc
    12481 gtttttcacg atctaaattt gcttgaacaa gcttttaccc attcatccta tgtgaatgag
    12541 catcgcaatc tgcaattatc ggataatgag cgacttgaat ttttaggaga tgccgtttta
    12601 gaactgatgg tttctcagta tttattccgt ttatatcctc atttgccaga aggaaaattg
    12661 acaaaaacac gagctgcgat cgttcgcgaa gacagcttgt cgaaatttgc caaagaatgt
    12721 cactttgatc aatatgtttt gttgggcaag ggagaagaga attcaggagg acgtacgcga
    12781 ccagcattgc tttgcgacct attcgaagct tttttaggcg cgttatatct tgaccaggga
    12841 ttcgacacga cccatgcatt catcgaaaaa gtgatttttc ctaaagtcaa agcaggtgct
    12901 ttttcacatg agatggatca taaaacaaaa ctacaagaag tcttgcaaaa atcaggtgat
    12961 gtatcaatcg aataccgatt gatcaatgaa gaaggtccag ctcatgaacg agtattttgg
    13021 atcgaagttt atgtagatga tcaattgatt ggcactggac aagggaaatc gaaaaaatta
    13081 gcagaacaag ctgcggcgga aaatgcattg gcagcactgt aaaagtaagt gagggaaagc
    13141 tttgtatttg aaacgaattg aaatagccgg ttttaaatca tttgcagaca agacagtcat
    13201 tgattttgaa aatagcgtca cagcagtggt tggaccaaat ggcagcggaa aaagcaatat
    13261 tacagaggct atccgctggg tattaggaga acaatctgct aaaagtctgc gaggcggaaa
    13321 aatgccggat atcatctttg ccggttccga tacccgtaag cagctgaata ttgcagaagt
    13381 cacagtgatt ttagacaata gtgatcatta tctgccattg gagtacaatg agatcagtgt
    13441 aacccgccgt tatcgacgga ctggggaaag cgagtttttc atcaataaac aaagctgcag
    13501 attaaaagat atccaagaat tgttcttaga ttctggatta ggaaaagaat cgttctcaat
    13561 catttctcaa ggaaaagtag aagcgatttt cagtagtaaa ccggaagacc gtcgtgggat
    13621 ctttgaagaa gcagctggtg tcttgaaata caagcagcgg aaaaagaaag cagaacagaa
    13681 gctttttgaa acagaagaca acctcagccg cgtccaagac atcatccacg aattggaaga
    13741 acagttaact cctttggccg cccagagtga ggcagcaaag gagtttttac ggctaaaaga
    13801 aacactgact caaaccgatg tctctttgat ggttgctgaa atcaagacag caaaaaaaga
    13861 ttgggacaac aaacaagcac agcttgcgaa attcaatcta gaattaggca aactatctga
    13921 gtcgatccaa gaacaagaat ccattttggc gaagcagcgt aaagaaaatg cgcaagccga
    13981 tcgtctgatc gaaaaaaatc agcaagtatt attagatctt tcggaaaaac tgaagcagac
    14041 agaaggacaa aaagatgttt tgcaggagcg gacaaagcat acacaaaaaa gctcgcaaga
    14101 atatcaaact tcattagcag aagcacaaaa aaaggttaaa cattttgaaa agcttcaaga
    14161 aagcttgatg aaagcagcag cagaaaaaga aacagagatc caaaaagctg aagcaaatct
    14221 cataaaaaca cagcaagaat tagaaaaata ccaaaaatca acgaaagaat tattggcaga
    14281 attaagagat caatatgttg atttgatgca agaacaagca gctgtaggaa acgaactgaa
    14341 gtatctagaa cgtcaatata tacaagaaac agcaaaaagc aagcagacgt tagccaagca
    14401 atcggaagta gaagcatcag tagaccgatt gatcttacaa aaagaagaat tgacacaaaa
    14461 gcaggcgcag ctaaaatcat ctttaactga aacaaaagag aagttagaaa tgatccaaca
    14521 aaacgggaaa aagttccaag aagcattagc aaaagaacag cctaaaatgt atcaattaat
    14581 gaatcaagtc cagcagctac gtgctcgtaa gaaaagcctt caagaaatcc aagaaaacta
    14641 ttttggtttt taccaagggg ttcgattggt cttgcagcat aaacagcagc tttcgggtat
    14701 tgtcggagca gtggctgaac tgatcgatgt tcctgcagat ttcacgttag ctatcgaagc
    14761 agctttaggt ggggcagccc agcatgtcat cgttgaaaat gaaaaagatg ctcggcaagc
    14821 catcacgtat ttgaaacagc aaagaggagg aagagcaaca ttccttccac tgacgacaat
    14881 caaaccaaga caattaccgg ctcatatatt gacacaagca gcagctgtag aagggtttat
    14941 agggattgcc agtgaacaag tcagttatcc cgatcaaatc caaactgtgg ttcaaaacct
    15001 gctaggaacg atccttttag ccaaagactt aactagtgcc aatgcaatcg cgcaaacgat
    15061 ccgctatcaa tatcgagtgg tttccttaga aggtgatgtg atgaatgccg gaggatccat
    15121 gacaggtgga gcgaataaac gaggaaacca aggcagtttg tttgtacaaa atcaagaatt
    15181 gaaacagttg actagtgaat ttgaagaagc agataaacaa ctccaagcac aagaaaaaaa
    15241 agttcaggag ctgcaacaag aaacagctcg tttagccgaa gaacaagaag ttttgcgtac
    15301 acgaggcgaa caattacgtt ttgaagaaca agaagcgaca aatcagctac aaaacatcat
    15361 aaatgaactt gaacgttttg aaaaagaaaa gcaaatctca acttttgaga cacgtgaact
    15421 gcagcagttt atcgaagatt acgaaaagca aacgaacgaa ttgaaagaca aacaaacaga
    15481 tcttgaatcg caacgacaaa aaatcgacga agaaatcaaa tcgttaagtc aagaaagcga
    15541 ccagatggaa gccagaagag cacaagtaca aagtcaaaaa gcacaagaac aagcagatct
    15601 tgctgtcttg aaagagcagt ttaatcatct gcagatccag ctccgcggtg cccgagtcca
    15661 aaaagcagag gcaaccgaac gtcaagaagc gatagaaaag cagttggcga cattgacagc
    15721 agacttttct gatcatgaag tcacagaaga aagtttggaa aaacaaatta acgaattatc
    15781 tgctcagcga gaaacattaa aagccgagtt ggcaaaagcc aaagagcagc gagatagaag
    15841 gcaaaaggag atcgataagc ttgaagcagt cttggcagag cgtaatcgtg agcaaaaagc
    15901 acgactgtcg gaacaatcca aattagaagt ccaaaaagac cgagctgaaa tgattttaga
    15961 taatcatttg acctatctgc agtcagaata tcagctgaca tttgagaaag cttcccaaga
    16021 ctatcaagaa acaaccgata tcgaagacag tagaacaaag gtttcatcct taaaagaaca
    16081 gattgaaaaa ctgggaccag taaacttaaa ttcaatcgaa caatacgaac aagtcagtga
    16141 aagacataca ttcttagcaa cacagcgaga tgatctgtta gcagcgaaaa accaattgtt
    16201 tgaaacgatg gatgagatgg atgatgaagt gcggacaaga ttcaaagaag tatttgaagc
    16261 tatccgtcaa gaattcaaaa ttgtctttcc aaatatgttt ggcggcggta aagctgaatt
    16321 agtactgacg gaaccaactg atttgctgaa aacaggtatt gagattgaag tacaacctcc
    16381 tggcaaaaaa ctgcaaagtc taagtttgct ttctggagga gaacgagcac ttacagccat
    16441 cgcgttgctc ttttccatta tccgtgtctg tcctgttccg ttttgtatct tagatgaggt
    16501 agaagcagcg ttagatgaag ccaacgtgaa acgttttggt cgttacttga gcgatttcca
    16561 agatgacaca caatttatcg tagtcacaca ccgcaaagga acgatggtcg cagcagatgt
    16621 actgtacgga gtgacgatgc aggaatccgg tgtatcaaaa atcgtttctg tacggatgga
    16681 agatatcaat gaagaggcga aaatcaacga aacgaaagtg taggaacgaa aatgatcaaa
    16741 ttagtagcaa tcgatttgga cggtacatta ttaaatgaac aaaagctgat ttcagatgag
    16801 aataaacaag cattagcaca agcaaaacag caaggggtca agatcgttct atgtacagga
    16861 cgccccttag ctgcgatggc gcattatctg caagaattag gactggtaga tgaaggtgac
    16921 tacagcatca ctttcaacgg cggtttggta caaaaaaatg atactggtga aatcatggaa
    16981 aagaaagtga tggaagtatc agatatccaa cgcttataca aactggctca gcaattggat
    17041 ctgccactag atgttttgtc tgacaatgtg gttttacagc tgcctagtgc cccaaggaag
    17101 caatccttgt acaatgtgtt gaataagctg ctgcaattcc aaccagcgga tataagcgat
    17161 attacagatg aatttgtgct taataaggca gtaatcgctt atggccaaga agaattagat
    17221 cctaaaatca aagaaatacc agcagaattc catgaaatgt atgagatcat caaaacaaga
    17281 agtatgcttt tggaatttat gccaaaaggt gttacaaaag cttatggtat ttctttattg
    17341 gcaaaagatc ttggtttgaa acaagaagaa atcatggcga tcggtgatga ggaaaatgat
    17401 ttaccgatga tccaatatgc tggactaggc gtagcgatgg agaatgctgt gcctttcgtc
    17461 aaagaagcag cagatttcgt gacagccagc aatgaagaaa atggcgtagc caaagtgatc
    17521 caaaaatttg tattagagta gggggagtca aatgggattt tttgataaaa tcaagcgtgc
    17581 ttttactggc gaagagaaaa cagaagaagt tgaagtacag gagaagtacg acaagggttt
    17641 agaaaagacg cgtaaaacat ttggtgaacg gatgaatgag ttgtttgcca attttcgaac
    17701 agtcgatgaa gacttttttg aagaattaga agaaacactt atcggtgcag atgtaggttt
    17761 tgaaacagcg atacgaatca ccgaagcatt acgtcaagaa gtgaaactca gaaatgcgaa
    17821 aaaatcggca gaagtccaaa atgcgatcat cgagaaactt gtggatctct atgaagcgga
    17881 aggaatcaat gaagtaaatg aactaaatat ccaagaagac ggactaacag ttattttgtt
    17941 tgtcggtgtt aatggtgtag ggaaaacaac aagtattggt aaacttgccc atcagttcaa
    18001 aaacgaaggt aaaaaagtat tgatggcagc agcagatacc ttccgcgctg gagcaatcga
    18061 ccagttagtt gtctggggcg aacgcgctgg tgttgaagtt gttcgtggca atgcaggagg
    18121 cgatccagca gctgtcgtat tcgatgcttt ggaaagagca aagacagagc aggcagatgt
    18181 tttactagtt gatactgcag gccgactgca aaataaagtg aacttgatga acgaattaga
    18241 aaaaataaaa cgagtaatcc agcgggaatt accagaagca ccccaagaaa cacttcttgt
    18301 attagatgca accactggtc aaaacgcgat ggttcaagca aaacaattca aagaaacaac
    18361 agatgtgacc ggtcttgttt tgacaaaatt agatggtaca gcaaagggcg gtatcgtact
    18421 tgctatccgt aatgaattgc atttaccagt gaaattggtc ggtcttggtg aaggaatcga
    18481 tgacttggag ccgtttgatc caaatgattt tgtcgttgga ttgttcaaag gattactaaa
    18541 agaagaataa cccatgtcta aaaagcagga gctgtgacaa aagttgcagc tccttgtttg
    18601 aatgattgtt tttaacatag ctgtgttttt gatgccttca tttttatact tatttgtaag
    18661 cggttctcaa acaatttcgt tctttttctt agactttttg attataatta gattcatatg
    18721 aattggaggt cacttaatga tgaattttaa gcaattatta ttgcatgtca atgcgcgccc
    18781 ttttatcgac caagctcgat ttggtataga acgggaagga cagcgggttg atcttgcagg
    18841 aaatctagca aaaaccgatc atccagcaat ctttggcgat cgatcctatc acccctatat
    18901 ccaaacagat tttagcgaaa cacaaacaga gatgatcacc cctgttaccg attctattcc
    18961 cgaattattt cagtatctcg ctgctgttta tgatgtgact gctcgttcta taccgaaaga
    19021 agagatgatc tggccattaa gtatgccacc tgccttaccg gaaaaagacg aagagatcat
    19081 tattgcaaaa ttaaaaaatt tcgaagatgt cttgtatcga cgttatttag caaaaaaata
    19141 cgggaagcga aaacagatgg taagcggtat tcatttcaat tttgaatttg gcgatgagtt
    19201 gctaagaaca ttgttcagcc atcaggaaga atttcaagat ttttccgaat tcaaaacaga
    19261 actttatttg aaaacagcga gaaactttat gcgctatcgc tggatgatca cttatttatt
    19321 cggtgcttca ccaatgagtg agaagaacta ctttttagat gaatcacatc cgcaagaacc
    19381 tgttcgcagt attcgcaaca gtgcgctggg ctatacgaat catccaaatg tgaaagtttc
    19441 ctatgcttct atgaaacagt atttagcaga tatcgagcga atgatcgaag aagggaaact
    19501 ttcggaggag aaagaatttt atacaccgct tcgtttccga ggcggaaaga aagtcgcaga
    19561 tttagcaaca acaggtgttc gttatattga attgcggaat atcgatttga atccttatgc
    19621 aagattgggt atcaatccag agcaagttcg gttcttacaa ttattcctga tgtacatgtt
    19681 atggacagaa gaaaaagaag actgtgacca atgggtagca gaaggtacga ctcgaaataa
    19741 caaagtagca ctagaacaac catcagatca aacagaattt catcaagaag gtagagaaat
    19801 tcttgaagga atgaagcaaa tgctggttga attggattgg ttggattctc tttatttagt
    19861 agaggaagca ttgactcaaa tggatcatcc tgaacaaacg ttagcagcga aactctatca
    19921 agaagcacag ctatcgagtc agcaagaagt tgctgtggca ttgggacatc aatattataa
    19981 agaaagccat gaacgtccct atcagttggc cggttttcgc gaaatggaac tttctactca
    20041 gatatttatg ttcgatgcca tccaaaaagg tgtccaagtc aaagtattag atgagtcgga
    20101 tcaatttttg cgtctgcaat tccaagacca tgtagaatat gtgaaaaatg caaacatgac
    20161 aagtaaggac agctatatcg tcccacttat catggaaaac aaaacagtaa cgaaaaaagt
    20221 cttaaaagaa gctgggttcc gggtaccagg aggcgcagaa ttttcatcca tggaagaagc
    20281 ggtaaaagct tatccgagat ttgccgagca ggcatttgtg atcaaaccta aatcaaccaa
    20341 ttacgggtta ggtatcacga ttttcaagga aggagctagt ctggaagatt atcaagcagg
    20401 tttagccatt gcttttcgtg aagacagttc ggttttagta gaagaattta tgccgggaac
    20461 ggaatatcgt ttctttgtga tcgacggaga agtccaagcc atcatgttgc gagtccctgc
    20521 taatgttatt ggtgacagta tccgaacagt gaaagaactt gttgaagaaa aaaatagtga
    20581 ccctttgcgt ggaacgaatc atcgtgcacc tttagaattg atccaattag gtgaattaga
    20641 acaattgatg ttgaaagaac aagggttgac gatcgaatca gttccccaag ccaatcaaat
    20701 tgtttatcta agggagaact caaatatcag tacaggtggt gattcgatcg atatgactga
    20761 tgaattttct gaagcttaca aaaaaatcgc cgtttctgcg gtagaagcac taggagctaa
    20821 gattagtggt atcgatttga ttataccaga taaagaaatc gatcctacaa ctgataaaaa
    20881 agcatatggg atcatcgaag caaatttcaa tccagccatg catatgcatg tttatccttt
    20941 tgcaggaaaa ggcagaagat tgacgatgaa tgtattgaaa ttgttatatc ctgaagtatt
    21001 ttaaaagaaa aagcttttga cagattgtcc tatttgatgt caatctgatc aaaagctttt
    21061 tttgcatgta aaaatttatg tgttttttcg ttttaaaata aacgatccca gaagcttttt
    21121 ttcttttttg gtttttcttt ttcagctggc gaagagtttt cttctgggta ttttttttcg
    21181 atatcaatca caggttcatt tactgcttct tttgctgtta agagcaatcc gaattcctca
    21241 ggatttgcag tgacgtaatc gttgactaca gtgaaacggc tattgttttt cgttgctagc
    21301 tggatatatt ggttttggac agaaaggggt agagccccat tcaagagtat ggaagcatct
    21361 ggatgttttt tgagttcttt taaaaagttt tctttgtttt ttggttggat catttcagca
    21421 acagtcatac taagatagca gcgttccctg aatgtcccta aatatttatg ctgttcttca
    21481 gggttcacta aaggtgtacc ataaagtccc ttatccaaat ggctttttac atcatcttgt
    21541 gccataaaaa ataccctcct tcttctcttt gtctttctta cgattatagc atattcccaa
    21601 taatacctaa aacatatgaa aaagcggttg cttttgttca acgattcact taaaataaga
    21661 atgaaaatca ttctcaatta gaaaggtgga ttggatgcgc tacggacgac tagtagcatt
    21721 attagttatt ttaagtagta tttcattgtt catcggagta tcgaatgtac aattgtcaga
    21781 actgctccag ttagatgggc aaagcttaca aatcttgcta gtgactcgtt tgccacgaac
    21841 gatttgtttg atccttgtgg gagcgaccag tagtatttgt gggttgatca tgcaacattt
    21901 gacccaaaat aaatttgttt caccaacgac tgcgggcacg atggatagtg cgcgtttagg
    21961 tattttagtg gctatgattt tcctacctgg tgcatccttg attgtccgat ccttgacagc
    22021 attttgcttt gcttttgcag gaacattgct ttttttatcc cttacgcgat tgttcctcca
    22081 gaaaaatcaa ttgatgcttc cgttgattgg agtcatgtta gggaatatca ttggttcagt
    22141 tgctactttt tttgcttatc aattccagtt ggtacaaaat atgacttcat ggcttcaagg
    22201 gaattttgcg actgtgatac gaggaaatta tgagttattg tatgctgttg ttccattgtt
    22261 gctcttggtt tggctatttg cttaccgatt tacgatcatt ggtctgggcg aatcgtttgc
    22321 agtaaatctg ggaatagatt accagaagat gcagttttta ggcattggct tagtatcatt
    22381 ggctagtgca gtcatgttga tcatggtagg aagcattcct tttatcggag tgatcattcc
    22441 gaatcttgtc tctagaatat atggagatca agtgcacaaa acaattggcg taactgcttt
    22501 agcaggtagt ttgttcttag tattttgcga tcttgtcgct cgcgtgttga tttttcctta
    22561 tgaaatacca gtcagtgtga tcgttggaat catcggtggg gctatcttcc tttatcttct
    22621 gataagggga cgacgatatg cttaaaaaaa agccatctat ttttattggt atgcttgttc
    22681 ttgttttatt tgttacagga atgtacttga cgatccatac atatggaaac tggcattttg
    22741 catgtgcatt gaggagtaag aaaatttttg catttatcct tgtctcgcta gctgtcagca
    22801 gtgccacaat cagctttcag acaattacgc ataaccagtt tttgactcct tcagtattgg
    22861 gacttgagtc gttatatgtc cttgtccaaa cggtcctttt cttctttatc ggaggagtga
    22921 cgatgcttca gcagacaggt acagtttttt tcttattgaa cgttgggctg atggtcgggc
    22981 tgagtctatt gttatttttc tttcttttga aaaaaatgag agcgaatctt tttttattac
    23041 tgatgtcagg gatgatttta ggtacgcttt ttggtagtgt cagtaccttt ttacaagtca
    23101 tcatggatcc gaatgaatat gatttgcttc aaggcagatt atttgcaagc tttggcaata
    23161 tccagagtca gtatcttttg cctgcttctt tgatcattat cggctgctgg tacctgttat
    23221 ttcgatatcg acatgtgcta gacgttttcc atctcggaaa tgaacaagca gtaaacctcg
    23281 ggatagacac agatcgtttt gaatttttga ttttatgttg tgtcagtctt ttgacaggga
    23341 catcaacggc tcttgtgggt ccagttactt ttttagggtt tattgtcgca aatatcagtt
    23401 atcgtgtctt ttctacttat cgtcatggtc agctatttat cggaagcttt ctggtagcta
    23461 tgctgtttct gacaggagga cagctcgcag tagagcagat tttcggctgg aatacaacag
    23521 tgagtgtcgt cgttgaattt gtcggaggaa tttattttgt cacaaaattg attcgcgaaa
    23581 ggaagagtat cctatgattg atttacaaaa ggtcaataaa ctgtaccatg gaaaatcagt
    23641 ggttaaagat gtacagttaa cgattcctga aaagaagttg actgctttta tcggtccaaa
    23701 tggtgctgga aagtcaacgg tgttgtcgat gatcagtcgt ttgataccaa aagatacagg
    23761 tgaaatctat ttagaccata acgaagtaaa ggcttggaaa tccaatgaat tagccaaaaa
    23821 attatcgatc ctacgacaat cgaatgaaat aaatgtccaa atcaccgtga aagaattagt
    23881 agaatttgga cgatttcctt atacgaaagg aaaattgacg acaaaagacg aggaaatcgt
    23941 tcaggaatca ctggaacacc ttgggttaga agaaatagcg gatcaatcga ttgacactct
    24001 ttcaggcggt caactccaac gtgcgtatat tgcaatggtg ctagcgcaag acacggatta
    24061 tattttactg gatgaacctt tgaacaatct agatatgaat tttgcggttc aaatcatgca
    24121 gattttaaaa aatttagtac aagaaatcgg gaaaacaatt gttattgttc ttcatgatat
    24181 caactttgct gcaagttatg cagacgaaat tgttgcaatg aaagatggac agttatttac
    24241 gcaaggaacg acccaagaaa tgataaaaaa agatgtattg gatgaattat acgaaatgga
    24301 catacggatt tgtgagattg aaggccgcag attctgtctt tatttctaaa actaacatta
    24361 ggagagaaga gatgaaaaaa ttagtagtag gtatcttgat tctagcatca tttggtttag
    24421 cagcatgtgg aaatagcaac aacaccagtc aggcagacac aaaaaaaagc agcacacaaa
    24481 ctgaaactac attgacgata acagatagta atggagacca aatcgaggtg ccaaataatc
    24541 cagaaaaagt cgtcgtattc gacaatggtt ctttagatac gatggatgct cttggtgttg
    24601 gagataaggt agttggagca gctacaagca gccttcctga gtatctttca tcctataaaa
    24661 aagtagaatc agcaggtgga ataaaagaac cagacttaga aaaaattaat cagcttcagc
    24721 cagatctaat catcatttct ggccgacaaa gagattttca aaaagatcta agtcaaattg
    24781 ctccgaccat tttcctttct ttagatgcga aaaatccatg ggaatctttc cagcaaaatg
    24841 tcactgcttt aggggagatc tttggtaagc aagaagaagc taaaactcaa ttagaggaat
    24901 tatctagtgc aatcgatcag acgaaaaaga aagcagaagc tactgacaaa aaagcactag
    24961 tcacacttgt gaatgaaggg caattatcag cctacggatc tggttctaga ttcggtttca
    25021 tccatgattt atttggcttt gaacaagcag atgatcaaat cgaagcatcc actcacggtc
    25081 aaagtgtttc ttatgaatat gtgctggaaa aaaatccgga tatcttattt gtagtagaca
    25141 gaacaaaagc tattggtgga gacgacagca aagatgatat ttctgccaat gaactggtag
    25201 cacaaacgaa tgctggtaaa aatcagcaga tcatctccct tgaaccagat gtctggtatt
    25261 taagcggggg cggtttagaa tcaatgaaac tgatgatcga agatgtgaat caagcattta
    25321 aatagcataa ataacagagg attaaactct catactatgt aacaataatg tttcttacat
    25381 tcttaaatag caagagccta tcctcaaaga agttatcaaa aaccatccgt aaaatcgtat
    25441 tgatgatttt acggatggtt tttatttatt tgagaaatct tttttcaacg tgaattcatt
    25501 atctacatca tacgggttga ttctagaatg gggagtggca aaaaatagtg tgatccattt
    25561 tttcagcatc aagatcgcct cctttgattt gaatcaaagg tacaaagcga aacttagata
    25621 aaacttaaat ttagatattt ttattttgta ccgatctctt ctttaaacga tgttccatct
    25681 gctgcttctc ggcaataagt ccactagact agaaaatatg agaagctatt ttctgtccag
    25741 cgttcttatt actcaaaaca aatcgcagtt gtcacaatct cttctaaacg atgttccatc
    25801 tgctgcttct cggcaataag cccactagac tagaaaatat gagaagctat tttctgtcca
    25861 gcgttcttat tactcaaagc aaatcgcagt tgtcacaatc tcttctaaac aatgttcaat
    25921 cagctgacct tcggtattag ctcaaagcac tgatttcaca acctctattt aggcatgtgt
    25981 ctcagcaagc ggttgatagt tgatgatctg catttttcga taaaaatcgg cgggaagctt
    26041 actccaccgt tttagtaatt gatagaaata actctcttct gctttgattt tatactcgct
    26101 taatatcaat ccaaaactat tcgttttttt tgaagctaat tttttataaa tatccaatgc
    26161 tttggtttta tcttcattga caaagagtgt ttttacttca atataagaac cctcgatatt
    26221 ttgtgtttct tcgataaaat agatgttttt tatatattcc ttcatagtga ttcctccaaa
    26281 gaagtctttt atttgataag tttactctac cgaagagttg ttaataatcc gtgaacaaaa
    26341 agtaattctt tttccaatag ttgttaaagg ttttttattt tttatagtat tttttgttat
    26401 ttaagaaagg aataaggtta aacaaataac aggttgcttt ttgggtcttt ttccgttcaa
    26461 gaaactgaac gaagaaaagg gaaagttgtc tcttttgtga taagagaaat cataataaac
    26521 gaatgactta ctttccaata aattgagaac agtcagaacc agacaaatta gttgcatctg
    26581 atgaataaag tgattattct atgacatgag gtgatacaga tgaaattaga agtgacaaaa
    26641 gaagcacaag aagtattgaa gaataaatta gaagcagacg atcaactttt attagatatc
    26701 gaaaacggtg acggtccatt tgcagaaagc caagtcagtt gccaattgga tacggcattt
    26761 cgattgatca ttgtcaaaaa agatacacaa acagatctaa gtctttattc tgtagtggct
    26821 gatacaccag ttggaccaat caaaatcaaa gacagtgcaa tactttacat ggatgatcca
    26881 tccacacttg ctttagaacc aacttataat agtttacaat taaaaggacc aagcggcttg
    26941 agaaaaggca acttgcaagt tgttcgaaaa gactagaggg ataaaatggg aaaattggcg
    27001 atcatcagcg acctacatgt ggacatcaat aaattcggcg aatcagagct gatgcagctt
    27061 tggcaagttt tgcaaaagca caagatcact cggcttcatc tagccggaga tacggctaat
    27121 aaagtagacc gttgcctagc tgttgtcgaa tttttcgtca ataaaggatt atctacgaca
    27181 tttcatttcg gcaatcacga gttggcagat gtgcccaatg aacagatgat ggaccatttt
    27241 ccagacccac attttttaaa tgaacggtat attgctttaa atgagcagac cgttctttta
    27301 gggatgaatg gctggtacga ctatcagttt tccgaattga aagattacga ggcaatcgta
    27361 cgaatgaagc gtttgtattg gtatgatcgg ttcatccagc gtgggaaaac agatctcgag
    27421 gtcaatgcag aaatgcttgc agctgcaaaa aatttgctag accagctgga agcaaaaaaa
    27481 cttgaggtga ttttatccac tcatttcgtt cctaaacggg catttatcat gtatcaatct
    27541 gctccttatg agcgttggaa caaattaaat gcatttttag gatcagaatc attcggagaa
    27601 ttacttgacc gttattcaaa tatcaaacaa gtagtttttg gccatacgca tcgtcgcttt
    27661 gaagataaat ccatcggtca cacgatttac agctgcagac cttttggtta ttattatgaa
    27721 tggcagctga cccgagattt tgtcttgaaa gaacagttgc tagacagcta tgatcctatg
    27781 aaattacgtg tcctcttgcg tgcccatcac ccagcttttc aggcctatca aacacaacat
    27841 ttgcaagagg aatttgaaca agcgatgaca atcgttccgt attgagacag cattagtaat
    27901 gtgtaaaaga aaataccttg taaaacataa ggataagtaa gagcagtaga tccatttatc
    27961 gtggatatgc tgcttttttt gcggaaaata atcagcgaat gtttatttga tcggatgatt
    28021 tgtaactcgc agagtactag atgcaagcgt gtattctttt tttgaacgat aatcacaaaa
    28081 aaaaatataa tatacatttt ttatttaatt ataattaata ttgattgaag tctaggcagt
    28141 caagtcacat tttgatagat aggaggggga cttacgaatt attttaaagg ctatgacagc
    28201 tgtatttcta gattgtgttt gcatcaagtt gcaataaaaa ctagaaatca aatcgggctg
    28261 ataatcattt ataactaatc gatacatagg accaaatata ggtctatgtt cttttatttc
    28321 ataagtatcg taaaacagaa ttttgctatg gattaatcca atcgactgat atgtttagta
    28381 aataaagaaa gaggaatgtc aatgatgaaa aaagaaatcg tattaacagc agcaatgatg
    28441 ttattcagca tggtagcaag tacgactttt gtttctgcaa ctgaagtata cccaaaggaa
    28501 tataacacag aaggaacgat tacatttgaa gcaggagatg agggtgtaac tcctcctgtt
    28561 gatccagaga atcctgatcc caataaacct gtcgatccat cagatcctcc aagccctgga
    28621 acaggagggg cattatcgat tgattatgga tcaaaattca aatttgggac tcaaaaaatt
    28681 tctactgcgg ataaaacgta ttatgcagct gctgatgtga tgaacgatgg gtctagaaaa
    28741 ccgacatatg tccaagtgac agataggaga agtaccttat ctgggtggaa attaagtgtc
    28801 tctcaaccag aacagtttaa aactgccagt ggggatgaac tagttggagc acagctgaaa
    28861 tttacgaaag ggcaggctgt ttctttggtt gatccaacat atacgccgca aacggtgaat
    28921 tctgaattaa cgttgacacc gggaggaaac aatacgttag caattaatgc taaatcagga
    28981 acaggtgtgg ggacttgggt ctatcgattt ggtgcaaatg aaaatgaaaa tcaagatgcg
    29041 gtacagcttt cggtacccgg taaatctgtc aagttagcgc aacaatattc cactaaatta
    29101 gtttggacat tagaagatac acctaacaac tagtttactg aaaagaactg ttaaaaataa
    29161 atacgtagtc atattgtctt gcgaggtaat cgagcaaaaa ttgtcaaagg ctaggctaaa
    29221 tttgtttgga tacgaatagt tcatctagga atttaacgat caggaggaaa agatatattg
    29281 aaaaagagga ggatcgtgct cattttaagc agtctcttag tgactaatat ctttccgatc
    29341 atagggtact ctttggaatt gaatcagttg gagcatggaa atagaagaga cgaagaaagc
    29401 aaactcactt cgacattcga cagtttaata gaatccaacc agacacaaga aagcagtgag
    29461 gatttaagta tttcgagtag tggagagacc actgacaacg tagaagctga gggttcggac
    29521 aaacaaggac tagaagatgc accaatttct gcggttgagc tgcctgacga tcccaatatt
    29581 atttctatcg ataaaatttt tcaaacacct atcggtacta gcacgtccat attggaaaat
    29641 gggaagttac tgcagttgaa cccagcagaa aggtctcaaa gaggagctat ttggtcaaat
    29701 aagaagatta gtttactctc ggattttaca tttaaatctt atttatactt aggaaatcaa
    29761 cgagatagag cgggagatgg catgaccttt acattgactg gcgatcctag aatggctagt
    29821 aatcccagtc aagttattgg atcgccaggt atgggaattg gctcatattc tactcgttct
    29881 ggccaacctt acattgcgaa tgcgctctcg attgagtttg atacgtataa aaatagtggt
    29941 tcttctgatc gaatggatcg agagataact gctgataata gagggcatgg acatgtcgct
    30001 tttgtcacgc ctaaaccaaa taataataat tattctggag agcatactgg cgtgacggta
    30061 gctcctacat ttctttctaa tggcacatgg cgcttgttaa taattaaatg ggacgcggag
    30121 caaagaagtc ttacttatga tttggaaggt gtcggtcgct cttctcggat tatacctgat
    30181 ctcattaggc agtttggttc tacagaagta aattgggggt ttacttcttc aacaggtact
    30241 ttttttcaag aaaatgctgt cgctgtcacg cgacttccat cgaatgtcac atcaacagct
    30301 gatgtaaaag tcaacgatag tgattatggg gccatcgctg aagctggagg aaacgataag
    30361 ataactatca gaaatacatt gaaggtcgag ggggattttc ttgaatataa gaaaatgcag
    30421 actagtatcg acctcccaca agaattaact attcccgagc atatcttgat caatggaaat
    30481 caaattgatg ccaaagatat cttgataacc ggaaatcgag tcacgctaaa tctgacagat
    30541 tattttattg ctggagatac gttaataatc gaattggaga caactttgca ggaagtaatt
    30601 cctgaaaaag tactcactat gcattttgaa taccttgaaa acgggaattt gattgagcaa
    30661 tcgaatgaaa taaacctaaa gatcgcaaag ctgaaggaga tagatatcca tgtttattat
    30721 aaagataaag acagtcagat tgatattgct gaaatgaaaa caatcactgg gaaaatagga
    30781 gagaagtata aagaaactcc tattgatatt gaagggtatg tttttgactc ggattcagcg
    30841 aatacagaag gcatcttctc cgaaaataca gaggatatct atttttatta cagaaaaggc
    30901 aaactttatc tctcaactgc tcctcaatat gtcaattttg gaaaacataa aatatccagt
    30961 agaccattaa gtgaattcgg acagttttca ggtgggttga aaattataga tgaacgggct
    31021 tttggaagtt ggaaattaca attgaaacaa gagaaagttc ttacaaatgg agattttgag
    31081 cttccagaag ctctttcctt tgttacctct gaaaatacaa cggttatagg gacatccgct
    31141 gttacgattt tcgaaagtga tcaaaagggt gaaagcgatt tgtcctctct tttagattct
    31201 acagctcatc gtggaatcag aataaatatt cctgtagaaa accaaagaat cggaaccttt
    31261 gaggggaaac tatcttggat acttgcagat gtcccaggaa attaacagga agagaagaat
    31321 ataacaaaat tattgagttt caagaagggc acaggtacca taggctcttg tgtcccacag
    31381 agacaagatt ttgttatagt cttctcttac atatgattaa aggagcgatt ttcttgagac
    31441 caaaacaaca attatttata acactcctag ttattttagg ggcacttttt aacaatatat
    31501 cagtgtcagc tagtgcattt aatttttcag tgacgccgat aacttcagag aatcaaatcg
    31561 acaagagaaa aacttacttc gatcttcaac ttgatccgga tcaggaagta gaagtaaaag
    31621 cggaattgag aaatgataca gaaaaagaag tgaaaatcga tatttctgtg aatagcgcaa
    31681 ccaccaattc aaatgtgatg gttgaatacg gaaaaaacga gattgaaaaa gacatgagcc
    31741 tgatctttga tctggtagat tatgtgagct atccacagat agtgacgcta aaacctaaaa
    31801 gtgtccaaac cgttgctttt catgtcagga tgccgaatga gcgatttgat ggtgtattgg
    31861 ctggtgggat cacattccaa gaaatccaga cggaaaagga ccaaacagaa actaaggatc
    31921 aaagcttatc gattgagaat gaatacacct atattattgc tttgttgatg caacagacac
    31981 tgaacgaagt tgctccgaac cttactcttc atgaagttaa accagatcaa atcaatgcaa
    32041 gaaatgtcat cttagcaaac gttcaaaatg atcaaaaaac ctatatcaat caagtagtta
    32101 ttgaaacaaa aattacaaaa aaaggacatt ctgaagtttt atatcaagag gaaaaagaag
    32161 gattgcagat tgcgccgaat acaaattttt cttttccaac tgctttaaat ggtcaaccgc
    32221 taaccccagg tgaatatcac cttacaatga ctgttttagg aaatgaaaat gaaaatggaa
    32281 aattttcgag aaaaaaagga aatactacga tcaactatac caatcaatgg gtatttgaaa
    32341 aagattttac aatagacgga aaagtagcca aggaattgaa cacgacggat gttacgatta
    32401 aaaaagacaa taattggatt tatatcttag tagcttcact tttcttattg tttattttat
    32461 tatgcatact tttgtttgtt ttgagaaaaa aagaagagaa agaaaagtaa ggttaagata
    32521 acaaatagta aatttttatt attgtaggat ggttgggctc tttttataac aatataaaat
    32581 ataccatttt attaataaaa atgcatacta gtaaatgata taattctaat aacaggtttg
    32641 atcgcgagaa aggccaaata aaacgctctc caaaatgtta aaaaaaagac aggacgatgt
    32701 atattataca aacaagaatc gctgacaaca gtgactcgga tccaggaaat cagttttatc
    32761 cccagctagt tggtgaatgc atggaaagta atatacgtag aaagagctga tgactaatga
    32821 attatgagtt ttttttaagc aaagaagatc acagaagtta ttcattactt aaatatttag
    32881 aagcaagctc aaatttatca gagtcaatat cgaatgttca agaagagttg tcgctatcga
    32941 catttctctt gaaaaaaacg atcgataagc tgaaacatga tttacaaaag ttcgatctag
    33001 agaaaaattt gcgagtaacc gtcagtgaat tggatatatg tctggaaatt aatggaaaat
    33061 gttctagcaa agctctgtta tctcgttata taacggattc tttatctatg aaaatggtat
    33121 tggctttctt tcaggaaaaa tatatctcag tagaaaattt tgctaaacag catcacgtga
    33181 gttattctgt ggcttataaa gtattacaaa gacta
//
LOCUS       DAZKOR010000023        32446 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000023 DAZKOR010000000
VERSION     DAZKOR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.023"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(96..1226)
                     /locus_tag="ACG8ZM_001003"
     CDS             complement(96..1226)
                     /locus_tag="ACG8ZM_001003"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0491980.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1286..1513)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1875..3077)
                     /locus_tag="ACG8ZM_001004"
     CDS             complement(1875..3077)
                     /locus_tag="ACG8ZM_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0491981.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3137..3340)
                     /locus_tag="ACG8ZM_001005"
     CDS             complement(3137..3340)
                     /locus_tag="ACG8ZM_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0491982.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3482..4549)
                     /locus_tag="ACG8ZM_001006"
     CDS             complement(3482..4549)
                     /locus_tag="ACG8ZM_001006"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0491983.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4607..4714)
                     /locus_tag="ACG8ZM_001007"
     CDS             complement(4607..4714)
                     /locus_tag="ACG8ZM_001007"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0491984.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4877..5689
                     /locus_tag="ACG8ZM_001008"
     CDS             4877..5689
                     /locus_tag="ACG8ZM_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0491985.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6063..6254)
                     /locus_tag="ACG8ZM_001009"
     CDS             complement(6063..6254)
                     /locus_tag="ACG8ZM_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491986.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(6499..6729)
                     /locus_tag="ACG8ZM_001010"
     CDS             complement(6499..6729)
                     /locus_tag="ACG8ZM_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0491987.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            complement(6713..6910)
                     /locus_tag="ACG8ZM_001011"
     CDS             complement(6713..6910)
                     /locus_tag="ACG8ZM_001011"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0491988.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7056..7934)
                     /locus_tag="ACG8ZM_001012"
     CDS             complement(7056..7934)
                     /locus_tag="ACG8ZM_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0491989.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7973..9091)
                     /locus_tag="ACG8ZM_001013"
     CDS             complement(7973..9091)
                     /locus_tag="ACG8ZM_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0491990.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9088..11187)
                     /locus_tag="ACG8ZM_001014"
     CDS             complement(9088..11187)
                     /locus_tag="ACG8ZM_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0491991.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11238..12059)
                     /locus_tag="ACG8ZM_001015"
     CDS             complement(11238..12059)
                     /locus_tag="ACG8ZM_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0491992.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12056..12865)
                     /locus_tag="ACG8ZM_001016"
     CDS             complement(12056..12865)
                     /locus_tag="ACG8ZM_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0491993.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12886..13380)
                     /locus_tag="ACG8ZM_001017"
     CDS             complement(12886..13380)
                     /locus_tag="ACG8ZM_001017"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0491994.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13392..13892)
                     /locus_tag="ACG8ZM_001018"
     CDS             complement(13392..13892)
                     /locus_tag="ACG8ZM_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0491995.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(13957..16653)
                     /locus_tag="ACG8ZM_001019"
     CDS             complement(13957..16653)
                     /locus_tag="ACG8ZM_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0491996.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17081..18154)
                     /locus_tag="ACG8ZM_001020"
     CDS             complement(17081..18154)
                     /locus_tag="ACG8ZM_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0491997.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18151..18978)
                     /locus_tag="ACG8ZM_001021"
     CDS             complement(18151..18978)
                     /locus_tag="ACG8ZM_001021"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0491998.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18979..19785)
                     /locus_tag="ACG8ZM_001022"
     CDS             complement(18979..19785)
                     /locus_tag="ACG8ZM_001022"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0491999.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19797..20882)
                     /locus_tag="ACG8ZM_001023"
     CDS             complement(19797..20882)
                     /locus_tag="ACG8ZM_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492000.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20899..21441)
                     /locus_tag="ACG8ZM_001024"
     CDS             complement(20899..21441)
                     /locus_tag="ACG8ZM_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0492001.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21699..22688
                     /locus_tag="ACG8ZM_001025"
     CDS             21699..22688
                     /locus_tag="ACG8ZM_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0492002.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22685..23422
                     /locus_tag="ACG8ZM_001026"
     CDS             22685..23422
                     /locus_tag="ACG8ZM_001026"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0492003.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23419..23970
                     /gene="coaC"
                     /locus_tag="ACG8ZM_001027"
     CDS             23419..23970
                     /gene="coaC"
                     /locus_tag="ACG8ZM_001027"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0492004.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23986..24618
                     /locus_tag="ACG8ZM_001028"
     CDS             23986..24618
                     /locus_tag="ACG8ZM_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0492005.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24703..25350
                     /locus_tag="ACG8ZM_001029"
     CDS             24703..25350
                     /locus_tag="ACG8ZM_001029"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0492006.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25355..26338
                     /locus_tag="ACG8ZM_001030"
     CDS             25355..26338
                     /locus_tag="ACG8ZM_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0492007.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26741..28255)
                     /locus_tag="ACG8ZM_001031"
     CDS             complement(26741..28255)
                     /locus_tag="ACG8ZM_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0492008.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28393..29199
                     /locus_tag="ACG8ZM_001032"
     CDS             28393..29199
                     /locus_tag="ACG8ZM_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492009.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29374..29943)
                     /locus_tag="ACG8ZM_001033"
     CDS             complement(29374..29943)
                     /locus_tag="ACG8ZM_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0492010.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30026..32086)
                     /locus_tag="ACG8ZM_001034"
     CDS             complement(30026..32086)
                     /locus_tag="ACG8ZM_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0492011.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
BASE COUNT         9719 a         6783 c         5517 g        10427 t
ORIGIN      
        1 taaataatat aaaaggtaaa taaaaaagag tggatttctc cactccatga aaagccgact
       61 ttttgggacg cttttttttg ccaagcctca catttttata gccaaacttc ttttgcgact
      121 tgctgcaata attggatttt cttccattgt tcttcttctg ttaaatggtt tccttcttct
      181 gttgaagcaa acccacattg aggactgata cttagatttt ccaatggtac aaattgttgt
      241 gcttctttta ttcgttcttt tattttctcc gtatcttcca aatctggaaa cttagaagta
      301 actaatccta aaacaacttt actttctttg ttactccaat gtcttaaagg agtaaaatct
      361 cctgctcggt cagaatcgta ttccaaaaag aaaccatcat aatttgttga aaacaactct
      421 tctgcaatgg gttcatatcc accggaaaac agccaagaag aagcgtgatt cccgcggcag
      481 atatgtgttg tgatcgtcaa atcttctggt ttgtcttcca gtgcgttatt gataatctct
      541 gtacaaatat gggccatttc tttcggatca attccttttt gaagaagtgc ttctctttta
      601 gtttgatcag ctagaaacgc ccaatttgta tcatcgaatt gcaggtaacg gcaacctttt
      661 tcataaaaag catgaatcgc tgctttgtaa gtagaggaca agtcctcata aaaatcagat
      721 agatcaggat aaatctcctt gatttttgaa gaaccatcat tagataggat ctcttgtcgt
      781 aaaatcatcg tgggactagg gatcgttgct tttgcaacaa aagtatcatc tgtttccact
      841 acttctttca gaaaagcaaa atcttttaaa aatgggtgat ttggattgaa agataattta
      901 tctactaccc gaatattata agctggtgct gcttttccgt ggaatgtttg attatatcca
      961 gtttctggaa catagccttc gataccattt agttcttcta aaaaatcaag atgccaaaat
     1021 ccacgccgaa attcaccatc tgtaattcct ttgatgccga ctgctttttg gtcttccact
     1081 aattttttta tttcctggtc ttcgattgca cgaagttctt cttttgaaag taagccttct
     1141 tgaacttttt ttcttgcttc tttgatcgat tgcgggcgta agaaacttcc gacatgatct
     1201 actgtaaatg gtctattttt gctcatttca ctcactcttt tctttgtttt ttgtaaaaaa
     1261 aaatcctttt actagcaaat actagtaaaa ggacgattat actcgtggta ccaccttttt
     1321 tcagaaaaaa cattctttct ctcagcctga caataaaatc agtggtcttg gtaacgaaag
     1381 actgttcgtt tttgcctacc tttcgacaaa aaatcctcta aggccatttt cgacagtcaa
     1441 cttctagctt tttacaccaa ccaaagcctc tcttgaacca ttttctgtct actttccttc
     1501 tcatcggata ctattagaat aaaattagaa tagcatgttt gaaagaaaag tcaataggga
     1561 ataagaacaa acaaaaacta atttgttatt cttagtaatt tgtttgtgga atacaataaa
     1621 gcttttctaa tatgataatt gttcagtcgt cctttcatct tagatcactt ccttaaaatg
     1681 atcatacccg tttcaaccac ataaaaaagg acttaatttt gaaaaatttc ccatcaaaac
     1741 taagtccttt ttattatagt tatgcaaatt tgcttatttt ctcgctattt ccgatacatc
     1801 ttgaacagga gcaattcgac tgaaaacact ggtgcaaaag catttatttg tcttatggta
     1861 gaaccgtgga aaactcagac tggtaataaa ttgtcatttt ttgaaagaat agcgccctct
     1921 tgaacattat caatcgcact taccacttgt tgatctgact ttgcatataa gtgaccgtaa
     1981 gttcctaaag tgatttgaat attcgcatgg cccaatcgtt cttgaataga tagcggttga
     2041 atattctgct caattagaaa tgcaacatga gaatgtcgta gcgcgtgaag cttaatcggt
     2101 ttgacacccg cttttttagc agcttctttt aaccagttcg caaaagaata aggtggcgta
     2161 aatgttccgt ccaactgagc aacatattca aaatttccaa tacgtttttg caagtcatac
     2221 cactgtctga gttttaagga aagcttcttc cccaacccaa tcgttcgata agcacattta
     2281 gttttgggag tcgaaaagta ccaatcatct tttgtattat aaatcaatgt cttctcaatc
     2341 gttatcgtgt ttttctcaaa atctacattc gaccaagaca aagcttcaag ttctccaatc
     2401 cgaatgccag tataaaagag catttcatac acaatcttcc gatggtgggt ttgaatctta
     2461 tcatccttca attcattcat cactttttga aactcactgt gttcccaaaa ctcaactttc
     2521 ttattctcag aaggaagcga agaaacatct tcaacgggat ttttagcaat caccccctct
     2581 ttaacagccc gatccaatac aatttttagg tgccctcgaa tccgattgat ataagatttt
     2641 gataacgttt cgccattaac ttttttccgt gaattacgtg actctgtctg gcttagttca
     2701 aataaccaat cctcaatatc gtgacttgta atatctcgaa cacgaaaatg atagaaaaac
     2761 tgcatacgct tttcgaaaat gaattgcgca ttctcatatg tttgactttt gacctgtctc
     2821 tcgtaccatt ttacaaacct atccttgtaa aactcttcga aagtcatttg attggtagaa
     2881 cacagttgat taggaattgt cttgttaaaa tcatgtgttt ccatgtactt gtcataagca
     2941 attttggcat ctttttgtga agcataacct cgtttagtct tctgaacctt tcttccattt
     3001 tcatctatac ctagatacgc tcggaagaac catttttttg tctttttatc ttggtaaata
     3061 tttttaattt ttggcatcgt tccattcctc cctgtacttg agaaaagcta ctttaatagt
     3121 accgcgcttg tactgtttat tccgcaactt gaatgtgaaa aagatgttca attactcggg
     3181 atggcaccac accaacttgg cgattattat aaaaatcaat accttcaatc cttgcaagat
     3241 atgccttgct ctctttaatc attcgtgcag cttgactagg tttaaagccc atctctatca
     3301 aaattgcacg atttaccatg aaatcacatt ttgtttccat tctctcacct cctgattctt
     3361 tactcctttt aaaataatcc tctttataat ctattgtttc tcattcgatg gttgtgttat
     3421 actcgtcttg tcgaggcgga gtacgttgca caacttgttg tgtaagcagt actttgcttt
     3481 tttagtagct tgttttctca tttgatatac tttgtaattt tcgttcataa tactcaacct
     3541 cctcctgata atactccggg tctgccttca tattttggag aagtttgatc tgtcttggat
     3601 tcatttctcc ttgttcaata atcaaaggca acaaggaaga atgaaacgtt tgatcgacct
     3661 gtgcaataaa aactaatgat ggtgaaacac catgaattaa ccaactcata gttttctcaa
     3721 aactttgagg aattgtatct aaagaaatgg tgattgcgcc ataattgcta ataaaggctg
     3781 cccatttggc atcaactttt gcacctagat catttggatc acggtcataa aaacaaattg
     3841 ctccattaag taactcaaag aacagtttgg ttaaatcatg ggtttttgaa ataatctttg
     3901 ccaagctttg cgctttctct tttcgatacc gtagttcaaa gcgattcttt acttttgcct
     3961 cttctaaagg aattcctcgt cgcttccgtt gttcatggtc cttctgataa aagcaaaaac
     4021 gacactgact cttctttgag cctaaataca aggtctcacc atttgattca ttgtttatga
     4081 tatccgtacc gccatgagat tcactcactt gaaaagtggt ccacacatgg cctagtatta
     4141 ccttatgctt aagttctgga atgctgagac tccccacaaa atcatctaaa gtaaaatcaa
     4201 tccgtgtaaa gttcccctga taatctaata ccttacgaaa aaactgttgc caatttgttt
     4261 ttcgtgtttt tagccatctt gctaagtgtt tacagccttg acctgaaaac tccatcacag
     4321 ttcctttaac cgcatcgtca atggaatacc gaatcgtgat tacttgattc cacacgtatt
     4381 ggccaatata gccctttggc gcataatcca gttcctcaaa acgttcaatg ggcatttcaa
     4441 taatctcttc aattagcttt aaatgttgat tcgagaaaaa gtgaatggtt agataatcaa
     4501 tcaaaacacg gttaactgga acgacctctt cttgcaaact atccaacaca agattcaatt
     4561 tttcttcact taattgtctc gttccttttt ctactttcga taataactat ctcgaaatcc
     4621 ccactttttc tgctaaatat cgttgtgtcc accccctttc ttctcgagaa actcgaatgg
     4681 cttcaccgag atcatgttga ttaacttggt tcatcgacaa acctccttct gctatttact
     4741 tgtcaaagaa cctattgaac ttattttaag tacattttta ttgtacgcct gtatttattt
     4801 taagtcaata tgttttgact ttttttaagt acacacgtat actaaattta agaacttatc
     4861 ctgaggagga gttttcatgg agatagacaa gcaagcagtc ggcaatcgaa ttcggcagat
     4921 tcgccaagaa ttaaagttga gtatggagaa atttggcaaa ctaattggtg atttaccgcg
     4981 aagtacagtc aataactggg aacgtgggat caaccttcca aaaaccgaaa cccttcacca
     5041 aatcgcagag ataggtcatg tcaccaatga atatcttttg tatggtgatc aagagaatca
     5101 atacatttta gaaatgctcc aaaaaaaggc aggtaagctt gatcctaaga ttgaaggtct
     5161 catagtagac gaaatcaaac acgctgacct tgttagtgaa aaagaaatgg atcgaatgat
     5221 tgaatttttt attacaaatc tcattccacc aactgaacaa gaccagttta ctttccaatg
     5281 tatcgatgaa aaaaagcagc tttatcttgg ttcaactaat tttggtaagg aagcccagct
     5341 ctatttgcaa catgataatc aaaatcatat tctgcacatg atgcccttta ccttctccaa
     5401 ctttagtgtg gatcgcatac tcgtctattt agcaaatcaa aaatcctatt cctattttgg
     5461 gaaacattta cccaaggaac taggtgagaa agccatcctt ctatactcaa ttaatcaatt
     5521 gaccagcgat gtgcgaattg ccccgttggt ctattcagaa gaaaccgctt cttatcagta
     5581 cacagaggac aatcaatatc tactagaaca gcaatatctt tatctaccat tcgtgatgga
     5641 agtcgagaaa gaacgcctgt taaatgcagc gtatccatca tcaaaatgag gtgacaaaat
     5701 tgataagcac gtgacaaaga aaatactgct aggacactcc ccaatgggta gttgtcacag
     5761 tttcctgttc tttgttagcc ccctgttaga accgggggct ttattcctgc agtccggcta
     5821 cgccttcctg caatagacaa aactccagat cgggtaattc gcttgtggcg aaacccgacc
     5881 tggagtttgt ctctgtattt tgaaccctaa aaattaatca aacagactga tattctcata
     5941 atcagcgcga aataagccat taagtacagt tttcctacct atttttgata acccgctggt
     6001 tagttaatat tccttttccg agagagataa atgattggtt aaatctatat acatctcaac
     6061 ctttaaaaat gttttcgaaa tacttcatca gtacatgtca tacaaattac cagtgcattc
     6121 tcaaattcta gttctttaat tgatttcagt tgaggagcag ttacttttgg cggaaatttc
     6181 catttttgtc caaacctctt cccccgatga aatttctttg ttgcaattat tgcaatgaaa
     6241 aatttttgtt tcaaaaatat acataattac ttcctccaat aaaaactatc tacctctcaa
     6301 attatttttc tctaataaga aaaacagaaa aggtaataag atacaacagt gatgattcct
     6361 aataaagaaa agactacaat aatatttttc taacttatcg ccttcaaaga aatagtttct
     6421 tatttttcac taaaataaaa aatttattgc aaaaaaaata gaagtgacaa aattatcaat
     6481 catttcaagg taatataact acctgagttt attatcgatt aaaatgctaa ctacatttgc
     6541 aagagaagcg agagttaaag gtaaatatag aatttctact tttttagcaa tagaaatagg
     6601 aataaaatat ttaaaatgta tagaaacttt cttttttttt cttgctatct ttttataatc
     6661 acaactctct ataatagaat tacgatttat tttatacggc tgaaaaaatc tatcaccaaa
     6721 aaaattcaaa cattaagcca aggattatga agtcaacatt tcgaaaaatg aacatacatt
     6781 ggtaagcaac tataactatt accacagaca taaagtaaca caatatatta ctatttaagt
     6841 gtacgccacc agtgaatatc ctcaaggtat acagcgcaca acaaaaagaa aaaaggaaac
     6901 gcagaagcat ttcctttcag tgatttaatg aaaacaacac gatttgactt agagcctttt
     6961 tatttgaatt cattttcgct ttatccaact tcatttgatc atttgattac tcggttaaat
     7021 acttttgaca tgagtatcaa tgaaacattc attaattatg gttgcttatt tcttaccgct
     7081 tttctggcca atgctaatcc gccgattatt ccggcattat cacctaattc gggcgtaata
     7141 atgtagctat caattggtgg tatttcaaca tatccattta taagctcaac aaatttatcc
     7201 tgaacttttt tcttcatgtg ttcttgtttc attacaccac cgccaaaaat aattctatct
     7261 ggcgaaaaca tcagtgttgt attgtatgca cattgggcaa ggtaaaaggc ttcaatctgc
     7321 caaaacgaat cgtcagccaa taaattctgg ccttttacac ccaaccgttt ttcaatcgct
     7381 ggtcctgctg ccatcccctc taaacaatct tggtggaagg gacagtttcc tgaaaaattg
     7441 tcctttggat gtcgcttgat caacatatgc cccatttccg gatgactgaa cccttccacg
     7501 aaagtcccat tgaacaaggc accacctccg atacctgttc ccacagtgaa gtatgcaaca
     7561 cctgctaacc ctttgccaga accagcaaca tattcaccat agcaagccgc attgacatct
     7621 gttgtccatt caatcggaat attgaaatat tgctttagcg ttcctacaaa attgaaattt
     7681 tgccaagcaa gttttggtgt tgatgtgata tgaccgaaac tcgttgattt gcattgaata
     7741 tcaattggac caaagcagcc gaccccgatg cctgccaatt gttccttaaa aggtttaaaa
     7801 aaatcaatga cctgcttcat tgtttcaact ggagttgttg tgggaaaact gactctttcc
     7861 aaaatcgtta attcctcagt accgactgca cagacaaatt tagtccctcc agcttcaatt
     7921 gccccatata tcatcatagg ttcctccctt cgaatatgtg aaaaacactt cattaacgcg
     7981 gcaagcgttc tcgatcagtg aaatgacgtt tagtatttcc tgaagcttct gttgaatacc
     8041 agtaagcgac actagcaaca tcgtcttgcc gctcaaataa gcgaacatca tcgttgccga
     8101 tttgttgtaa agtgaccgat aaatcttcgg taaaacagat ggggtcttga agatgccaac
     8161 gataaagacc atgtcgtggc attgcgtcgt caccaaaggc atgaacggca ttcaattttc
     8221 ccgtttcaaa acgatctctt gtggcatctc tatttgattg gtaaggatac cctaaaaagg
     8281 ctgttgaata gtttttcgca tgtggacgtc cgaactcatc gtactcatga aaggcccacg
     8341 ccccgccaaa ataatcttct gaacctgttg aggttacggt cggatactca ctatccccat
     8401 caatgtagaa tttaaactcg ccttcgcccc accaataacg ttctaggcag gtaagcgcaa
     8461 ggtaagtacc cacgtaatag ccttgaccct ttaatcgatc aattaactta taatcctcct
     8521 gtaatttggt aatgcgttcc cgatgccaat ttgcgtgaaa atataggcta ttttcttcca
     8581 attcgtccac caaagcgtaa tttatcgtat aaaaaaatga ggacatatcc ttcggatgta
     8641 aattctcgat cgtaatttta gcggatgttt ggaaaggcat ttcgaaatag gaattaaagc
     8701 cccctgtagg attaaccact atcggcaacg agttcacctc gcaacgctct ccaaacccat
     8761 tacaaaagaa atcccctaaa ggactttcaa cagcagggct atccgaccca tcccaataaa
     8821 ttcttaagat taaatcacgt aaaacaaaac tgccaaattc tgttcgatca cgaatcgtca
     8881 tccacatatg acgaattatt cccgttccag taatatccgc aatcacagtt tcttcaccag
     8941 aagtcagcgg aattgcgcca cgacctttgc ggcttggtcc tagctcgctt ggcaacatcg
     9001 cagctttacc tttctcgcct gttggatttt cgggagtgat tgctcgactc tggatttttt
     9061 taaattgggt aatatttttt agtatactca tctttttctc ctttgaatgt gaatcgtttt
     9121 tcccttttct acgtgaagaa tttttttcgt atcacttatt ttcaatggct tttctgacaa
     9181 atttgaaata ctcaactcgg attgcgtaca ttcaatcgaa atgaaggctt gaccgaactt
     9241 caaattcttc caagaaaacg ttaaattatc aacaggatca atttgcaata ctcctttttt
     9301 gaatttcaat ccgaaatatt gactgatcat taggctgaca aaaagacccg atgcccagcc
     9361 gcatttgccg cagccaaagt ctcgcacgac ttctccttgt tgagcctcta agcgatacgg
     9421 ccaccaccac cacgaaccgt ctaaatctgc caaacaaatt agttcttcta atcgggttac
     9481 gaaagcttga gggttgtgca gctcgctcat taaaacagtg ataaagccag gaaaagtggc
     9541 gccggattct aatccccaag tgatgccttt gatttcctcg ctataggttg gattgaaaga
     9601 actagcagaa aaggccatgg tgttctgata taaatgatct ttattatcta aaaattgata
     9661 aaaaggcatc aacgtagtgt ctgattcttc tccatcatgc atcaataaat taatttttcc
     9721 atccacaatc acatcactta aaaagattaa cccttgctca agaatcggtt tttgatagtg
     9781 cttcactgaa taggttgttt tctcctcatc accgattccc tccaaaaatt gctcaccaaa
     9841 aggaccatcg atagtcattt gtttacaaat tgattccttt agtttacagg caatgttttt
     9901 gtatcgcttg ctaagactca gttgttctag ggcttcaaaa atttctgcaa gtccaataca
     9961 ggccttccaa agacaaagat tgctgccggt atgatacttt ccaagtgcaa aggcgtctga
    10021 taaccaaaca gatggaaaaa gcattggttc ccctgaccga cgttgagcta atatgtcctc
    10081 aataattctt gaagcattca gtagaacctg cggacaccgc tcaaagaatt ttgtatcccc
    10141 agtatgttca aaataaagac tagccagtaa cgcactggat aacgaattac tcagagaatg
    10201 attgatacct cctgtaaatt ttgaaccagg aaacttaacg ccatattgat caaaccatag
    10261 aaccgttttt tgacataact ccggttcaaa catggtgaat ggcaatgatg cgtaatacat
    10321 atccttattc caaattcgat tagtagcagg atagctgccc caattgctgc cgacgacctg
    10381 tccctgtcga ttcataccga aactagaaaa gctttgataa agtgcacgac gatagagatg
    10441 gacaactcta tcgtttggca ttgacagctg cccaaatagc tgagcaaaat agttcaatgt
    10501 ttccttcagc catttttcga tatcagattc ctgaaaagtc attacttctt caaaagcatt
    10561 tgggtccaca aaagcaatgg cgaagcttgc cacttcctgt tgtgcgatcg tgtccttatt
    10621 tcctttttga gaaatcaaca cattttgctg atccgaatac ttctcttgat acgaaggcac
    10681 atctttcact gaaacttgaa ggctttcgtg tgtttcattc acaatgtaaa cctgttgaac
    10741 aagcatactt aaacgcttat tagccagtat gggagcaaag gatactacaa tgaccttaaa
    10801 cgtcgaataa tgatgaatat atctaggcaa acagtcttga atcacatcga cttcaatacg
    10861 atgatccgat tctgacaaat ggtagacaat cccgtttact tcaaccttta aggataacga
    10921 attggtttga gagagctgtt tattgaccca aacacctggt ttttcctcag ttagtaaatt
    10981 ttctcgataa aaggtcaacg tttgaatcgt cccgcaaaca tcaaggtttg ctaatgtctt
    11041 ttgattactt acatcgaagg tcaatccctt tacatcagga atattccgat agtagctgta
    11101 ttcgtttgaa gtagtcacat ttccgtcgtt taactcagca ttaaaaaatg ggtgtttggg
    11161 tgaataaatt acttcattaa tagtcatctt ttccctcctt gctaaaaaaa ctgcagccta
    11221 gtagctgcag cttcctacta tgcgattcca agtaatgaca ataaaattcc caagacgacc
    11281 attccaaaaa taattttgat gacgccgact tttttatcta gtaatttgaa acagctcaat
    11341 gttaccaata acggtacaat tcctttaaaa atactatcga ggatttcttg gacattcatt
    11401 agttcttcgc ccttcattga aaagctcaaa acggtattga atttgaccat actgatcgtc
    11461 attgcaccta ccatgatcag acccaagata ctagcagctt ttgtcagaat ttcaattaac
    11521 ccgttcgcat acagcttttc aatatattta gagcctaatg aatagcctaa aacgccgcca
    11581 tagtatcgca tcaagtaatt aggtatattg aacataacta aaaagataat tggtgccaaa
    11641 atatttccgg cattggctaa gttgatcgct agtcccgcgg taacgacacg ccaaactccc
    11701 cagaacaatg aatcgccaat tccagccaat ggtcccatca aactcgtttt gatcgcgttg
    11761 atactttcca cgtcaaaacc agctttttca gaattttcct tctccatcga tgccgcaatc
    11821 cccatgacaa aggacgcgca aggaatggtc gtcgaaaaat actgtgtatg acgttccagc
    11881 gccgcagctc gatcttcttc acttttgtag aaatgattaa tgaagggcat catagaatag
    11941 gcgaaaccac cgccgcccat cgtcaccgga ttcaccgaag catacatagt catcgagcgc
    12001 caaaacatct tccgaaccaa ttttttctca tctttagaaa ttccatcagc taatttcatt
    12061 gaaagaaatc ctcctcttct tggtcaagcg ttgcatttac agcagcatcg ctttgaactg
    12121 catgaacaga tatatttttt agttgtagat ctctttgggc tataacaatt gcaataatcg
    12181 ttccaactgc ggcaattgca atcaatggta actcgagata cgccataaca acggccccaa
    12241 agaaaaacca cacagcaata tttttactcc aaagcatccg taacagcatc gccatcccaa
    12301 ctgccggcag cagatttcca gcgactgtta atccgttcat gatgacctct ggaatattat
    12361 ccaagacgct tttgacaaca tctgctccaa ataaaacggc aaagaatgga ataagagcat
    12421 aaagcgcata gtggacaaac cacataaaga agtgcagatt gatgattcct ttttgatcac
    12481 cagattttgc aaatttgaaa aatttagcag ccatcggtcc acatacaaaa gaaaaaatca
    12541 tattcattaa ctgtaatcct aatactgcta gtggaaagac aatgggaata atcgcatcca
    12601 cgtttcctcc cattttgatt gcgaacgctg tagccagccc agcagccaaa gaaggctccg
    12661 ccgtactaga gattccgata ttgacgacac ccataaagat ggcttctagt gacgccccga
    12721 caatcaaccc cgtctgcaaa tcccccatca acgctcctgc taacggagca acaacaatcg
    12781 gacgactcag catggacatc ccgaacattt catgtccagc aactccaata aaaacggcca
    12841 aagcgactac aaatgcatca tacataattt ttctccttcc tttttctaaa aatcactcaa
    12901 aatagagata gctgaacgtt tttccatcgc cggtgtaggc tgaaaaacgg tatctggata
    12961 aagttcaact aattctttta agtcttgaat ttctgacgtc gttaacatca cttcctttga
    13021 taaggttttc ttatcttccg atggcggtgt cattttccca gcattcccca cgttcattaa
    13081 ttcgatttcc gttgggaaag ctttcgcaac ttttaccgca tctttgacat tagggactaa
    13141 aaccatcaag cgcatgtcct tcgcccgcgg atcttttaac aaatgaatcg cgtcatcaac
    13201 tgttttaata atgactttga cattcgatgg tgcagccatc ttcaatgtca tcttttgaat
    13261 atcgttggta gccgcctcat cgcttgctac tactaacccg gttaaattta gactctttgt
    13321 ccaagtcatc gctacttgtc catgaattaa tcgttcatca attcgcactg ctttaatcat
    13381 ttttcttcct cctaaaaaaa atcttcctct tcatttacta catgtacctc atcaagaatg
    13441 gtcatcttta attcctgttg gcatttattt accagttcct gaatatcctc ctttgttaat
    13501 tccttggttg ccaatactat ttcgagcaga attggcagat taaagccaga caaaatagtt
    13561 acagcaatct ctttctccaa aaaggttttg gtaatcttct gattgacact tccgccaaat
    13621 aaatcggtaa aaacaaaaat ctgatcctgc tcgttcacct ggtttacaaa tacattcaac
    13681 tcctgttcaa aatcacactc atccacataa gcattaatca ccgtaatctc ttgaaccttc
    13741 cccgtcagta attgaatact gctaagaaag cctgaagcta agtgaccgtg agtcgctatt
    13801 agaattttct tcatatggtt aattcctttc tttctgttca cttttatata agcaattcct
    13861 gtgccaactt ttaaaaacat tggtttaatc acgttaattc acattaaaaa aagtgtctaa
    13921 cctaaaaaaa acagattaga cactttttta gacactttat ttttgaatat acggtcctaa
    13981 taattcatag agcagcgaaa gctcataagc attgacttta aaattatatt tcatctccat
    14041 tgattgaaaa atacttctag aaacataata aaattcatct tcatctggtt tcaaatcata
    14101 attcggttct tcagattttt ctgtccgagc caagaataaa cgttccatca tcaatgccgt
    14161 atgcatatag agatttagtt ttacacggcc atttaaagtg aaatgatagt aattttcata
    14221 tttagaaatg actgtttcca cttcactcat cacgatcgtt gggttcagaa agttcaaacg
    14281 gtccgcaatt ccttccaacg aaaagaattt tactagctcc tgaatcaagc ggttaaattc
    14341 tgccggcttc atttcaggac tcaaaatatg ctccaaattg gctgctccat caccgtctaa
    14401 gatgtcataa acattaatat taggaattga aaaggaacgt ggaagatccg aagtcgtgat
    14461 gaccaatttt gtcttgtcaa aataagtttc atcattttgg tcgattaatt cacgtagttc
    14521 tctgtagtcc ttcgtgaaaa cttcgatgga ttcatccgat aaatgatgac gaaaaacatc
    14581 tttcaatttg tccgaaatcc ccaaacccga catacaggaa atgataatgt tgctactttg
    14641 agaaaagcct tcaaagtatt gaacattgat tgaatacttg tctgctgcat cctccgcaat
    14701 ctgtttaaat ggcgcattca tcgtcatctt caagccaata tcaagtgcaa tggaagtcgt
    14761 caaattattc agaagaagta actccccttt taaatcgttc ttgatctgtg tgtaaatttg
    14821 atttaaagag cccatatcta cgatcagcac tagactatct gtattcactt gccgtttcac
    14881 gaaattcctt gtcgattcga caatctctga cacattactc tctactggca tatccaacgc
    14941 ttcaaaaaca taattgccgc ataattgatt cactactgct tgaatgctgc tagccgtact
    15001 atctccgtga gcaagcaaca ggcccttcag ctcgatggat tcatctacat aatcagataa
    15061 agtcacgaca ataagcagtc ttagaaagtt ctcgtccttt tctgagagcg gtggtaaatg
    15121 ctccaagaac tgattacata agtaagtcgt ccgaggtaga tttgcagcta atgtacgatt
    15181 tatcccggtt aagtctgtct tatagcttgg ggaaatgaca taggatctgc taataatttc
    15241 tgcaacaggt ccactcgtcg tcaatccgta cgtttttcct aaaacggata cccagacctt
    15301 tctaaacatc tgaatcatca attttctact cattgataaa aaagaatcct tatcaatgtg
    15361 atgcaccaaa tgctgtactt cattctttat cgaagaaaaa ttttcattga aattaccttc
    15421 atttatcagc tgagtaattt tttccaaaat cgaatcataa tcaaacggtt tgattcgatc
    15481 cgtttttatc ttttgatttt tctttatcaa taaactagca atcggcaagc tttctacttc
    15541 ggattccagc atatttaact gaatcaagat ctcatttttc ccaatcgtta atggttcttc
    15601 ttgtgttcga ctatacacat ccgcacaact gattttaatg atattcttca gcttaccaat
    15661 attcccacta aagggatgag ataacattaa agacaaggct tctcttgaaa caataatatc
    15721 tttttgtaaa acatccgctt cattttgaaa aaataattga atcagctcta atcgttctcg
    15781 aatggggcga tcttcaaaag ttggcaacat aacactaatt tgaatacgcc gatcaaaggt
    15841 gtctaataaa taattttcac ctttttccgt cgtggcgaaa ataaaacgaa ccttagcaga
    15901 atgccaattt acattttcac cgactggacg atagtttcca gtatcaataa atagaaagag
    15961 cttttcttga ttttctttcg ataggcgatg gacctcatcg agaaaaagat agccgttgtt
    16021 ggccgttgcc aataaaccat cggtatcctt ttcagccccc gtaaaggccc ccttcttgta
    16081 tccaaacagt gttgccgaaa gcagttcggg attgtccgca taatcggcac agtttaagat
    16141 agcaaatggt gcatcttttt caataatttg ctcatggata gcgtattcaa aaatttttgt
    16201 ggctaaaaaa ctttttccaa cacccgtggc tccatttatt aatacactga gtccgttagg
    16261 cggatatttg acagcggcta tacatttttg aatgacctct ctcagacttc cgtcatgacc
    16321 aactacagaa tgaaaggctt cgtttatttg ctccgtatta tctacaaccg catattcatc
    16381 taaaggcttc cacaaaacag gctttcccgg taatttctcg acctttttat cttccactaa
    16441 tctcgtcaaa taatggctgg cattctgacg agagatacct agattcttcg aaattttctc
    16501 ggcagtgaca gaagacatat tccctgttag tcgtatcagc tcatcaaaaa ctcgatcttt
    16561 agcgctcttt ttcatttttt cctccagttt aatgtgtcta aaagcataat aaactatcgg
    16621 ctgcaatatg tctattctat atttgtattc caatttttac tttgaaacac aaatagtcga
    16681 atatttgcct cagatatctg acaaaataat ttatctacca atttagtttt tatctaatat
    16741 gatttttatg ttttttcata gctacaaata ttgaactgga ttcatatatt agttttatta
    16801 atagattaca gaagggttaa gtatatttgc atagtttcca attactttat ccttctgtaa
    16861 ctttttcaat cttagattta ctagcccgtc tcgggcccta ttttacaatg gttttgtcca
    16921 cttttctctg gtagaaaaac gtggtagaaa aaataaaata acacaaaaaa agcggcgttt
    16981 catccgcttt tggtagaaga ttggaaaact cagatagaaa aaggacgatt tttagctctt
    17041 tagaacgttg ataagtcccc attcttactg agttttgcca ttatctccta tacatcttga
    17101 attctaagaa taggtcatta tagatgccta ggtatttcga tcctagatct ttgtagactt
    17161 caagatggga gatcgtgtca tcagatggat agaattgttc gtccgacgaa atgtcttttg
    17221 gcaataacgc ttttgcttct tcatttggtg tagaataacc aatatactct gcattttgtg
    17281 ccgcattttc cggttctagc ataaagttga tgaaatcata ggcaccttct ttatttttca
    17341 cggtttttgg cataacgata ttatcaaacc aaagattgga tccttctggt gggatcacat
    17401 aatgtaaatg ttcattttcc cacatcatat cggcagcttc gccagaaaat gtcactgcca
    17461 cggaggcttc ttcattgatc atatacattt tgatctcatc tgccacgatt gctttgacat
    17521 ttggtgttaa atgattcaat ttatttgctg cttctgtcag ctgtgccata ttcttactat
    17581 tcaaagaata gcctaaacta ttcaaagaaa gccccataac ttctcgtgca ccgtcaatca
    17641 gcatcaaact gtcttttaat tccggcttcc acaaatcatc ccaatgctta atttgatttg
    17701 catcaaagaa tttatcgtta tatacgatcc ctaaagttcc ccaaaaatat ggaatcgaat
    17761 actgattatc tggatcaaaa tccaagtcta aaaatcgtgg atcaatatta tttaatcctt
    17821 tgatcttcga atgatctaat ggaagtacta gtttttcttt gatcattttt tggatcatat
    17881 attcacttgg tatagcgatg tcgtagtttg tcccaccttg ctggattttt gtgaacatcg
    17941 cttcattaga atcaaatgtt tcataattca ctttgtatcc atattctttt tcaaatttcg
    18001 tgatcaaact aggatcgata taatctcccc agttatagat ggtaagtacc tttgctccac
    18061 tcatgccact tgttttttca agttgacgaa caccaagcag cagcaccgca ataatgatga
    18121 gaatacctaa aaacagagat tgtaattttt tcatcgcagc cgtgccaccc cctcgcgtac
    18181 tcttctgttt ttcttcagcc gttttggacg gttttcttta ctaataaagt agtatccaat
    18241 gaccaatatc atggagaaca agaaaactaa cgcacttagc gcattgattt ctaaactgat
    18301 tccttgtctt gcacgagaat agatctctac tgagagcgtc gtaaagccat ttcctgtgac
    18361 gaaaaatgta accgcaaaat catcaagaga ataggtaaat gccataaaat atccagctat
    18421 gattcctgga gaaagataag gcaaaatgat atttttgact acttggaaat tattagcacc
    18481 aaggtctcta gccgcatcga ccattgaatc gttcatttcc tgcaattttg gcaagaccat
    18541 caagacgacg attggaatgc tgaaagcaat atgggaaagc aatacactct gaaaacctaa
    18601 tccgaatcct agtagtgtaa agaaaatcaa gaaactagca ccgataataa catcaggaga
    18661 gactaataag atgttattca tgctcaacaa cgctgtccgt gcacgtcgtt ttttcgtgta
    18721 atagattccc atagccccaa atgtcccgat gatcgtggcg atcaatgcag ataagaaagc
    18781 cagcatgaac gtattcaata cgataatgat caaacgtgta tcttcaaaaa cagcttcata
    18841 attttcccaa gtaaatcctg taaagctgtt catcgttcct ccttcattga aggaataaaa
    18901 aatcagatag aagatcggtg catataagaa taaaaagacg atccctaaat aaagatacga
    18961 ccatttaaat tttttcattt atgagcgacc tccttttttc ttctcgcctg tcaggagcat
    19021 cacgatgaac atagcaatga ttaaaatcac accgattgta gaacccatcc cccagttttg
    19081 tgtaaccaag aaatgctgtt cgatagccgt tcctaaagtg atcacccgat ttccgccaat
    19141 cagacgagtc agcatgaata aagaaagaga aggaataaat actgcttgga caccactttt
    19201 cacaccattt aatgataacg gaaaaatcac tcgtcggaag gtctcaaagc tattcgcgcc
    19261 taaatctcta ctagcactga tcaaagaagg attcatctct tccaatgcat tgaaaattgg
    19321 cataatcata aatggaatct cgatatacgt tgccacgaac agaaaactga agtctgtgaa
    19381 taatatctgt ttcggcccga tcccgataaa atcaaagaaa tgattcacgc tgccgtgcac
    19441 actgaaaatc ccaataaatg cgtaagcttt aagcagcaga ttcacccatg taggcaaaat
    19501 aatcagcatc agccataatt gcttgtgctt cagttttgtt aaaaaataag cagtcgggta
    19561 actgatgatc agcgtgaaaa acgtgatcaa aaatgcatac catacagaat tgatcgtcat
    19621 agaaagcgat gtccccgacg taaaataaga tagataatta ttcagcgtga attgcccatt
    19681 catatcaaaa aaagattgat aaatgatcat taatacaggc gcgatcacga ataaaccgag
    19741 ccacaaaata taagggacgg aatagatcct gcgcattgtt ttcattcttg tcccccctat
    19801 tcttcgtagc tgtcaagacg tgcatcgaat tcttcttcag actcgttgaa acgcatgaca
    19861 tggatatctt ctggctcaaa ggccaatcct actttgcttc cttctttagc ttttttcgtt
    19921 gaatggacca tccattcatt ttgctgttca tcataacaga tgatttcata atggacacca
    19981 cgaaacagct gagtatcgac cgttacgact agttttcctt tatctggtgt cgtgatcgtc
    20041 aaatcctctg ggcgtaaaac aacttcaact ggttcattta gacgcatccc gccatcgaca
    20101 cattcaaatt gcttaccaac aaactcaact agattatctt cgatcattac accgtccacg
    20161 atattgctct ccccaacaaa atcagcaacg aaatgattga ttggctcatc ataaatatct
    20221 acgggtgtac cactttggac gattttccct ttattcatga caaaaatctc atcactcatt
    20281 gccaacgctt cttcttggtc atgagtaaca aaaataaacg tgattcctaa tcgttgttgc
    20341 agatcccgca gttcatactg catatctgta cgtagtttca aatcaagcgc cgataacggt
    20401 tcatctaata gcaatacttt cggttcattt acaattgctc gtgcaatcgc tacacgctgt
    20461 ctttgaccac cagacatttc actaatctcc cgtttttcgt atccaggaag ctggaccata
    20521 cgcaaagcat cttttacctt cttttcgata tctgatttgg acattttctt gatcttcaat
    20581 ccaaaagcaa cgttatcaaa aacattcata tgaggaaaaa gtgcataatc ttggaagact
    20641 gtatttactt gtcgcttatt agctggcaaa tcattcaacc gtttcccttc aaaatagatg
    20701 tccccttctg tagcttcggt gaatccagct ataatacgta ggatcgttgt tttaccacag
    20761 ccggaaggac caagcagtgt ataaaatttt ccttgttcga tctcaaaact tacttttttt
    20821 aatactgttt catcatcata gcgtttcact acttgatcaa atgaaatgat tggtttcccc
    20881 acgtttttac cccctatatt acaaatatga ttgcgttgca acaatcaata cttttgttcg
    20941 ttttttgaga tgattcctta attgatgttg ttttgtcgct tgataataga tcgactgtcc
    21001 tgcttttgcc acatagaccg tctctcctaa gcttaactct aattcccctt ctaatacaaa
    21061 aataaacgtt tcagataatg aaggttcaaa cgtcttatat tctcctgcac cttcaaaagt
    21121 cagcaagaca ggctccattt ctttttcatt cgaatcagcc acgagccatt ccaattcata
    21181 cccgttttct tcatcgaaat aaaccgtatg atcttctttt gaataaacca cttgcagatt
    21241 cgctgcttct tgatgaaaaa attcttctgg agtcacacct aagacttcaa gaatagaaaa
    21301 gaatgtttcc attgatggtg aacttaaatc tcgttccaac tgggaaatat acccttttga
    21361 caaatctgtg cgttcgccta gttcttcttg tgtcaaattc ttttggatgc gcaaatttct
    21421 taacttctca ccaatttcca taatttcctc cgaatcgaag ttttctaata gtaaacttcc
    21481 tgtttactac cactcgtatt agtatacgaa atttattgaa aaaggcaacc ctttgtaaaa
    21541 ttttttttgc tgaatatttc tttatctttt ggaataaatt ctagttgttt gattctttat
    21601 ttttcaccta atttttgtct aaatttacgg aagaacaaga ataaaagagt aggctttatg
    21661 gtatgataaa agaaaatttt aaaaggagtg tctcacgaat gaacgctttt cgatcttttc
    21721 aggtaataaa ggaacctgtt ttccatacag caatcagtga tctgccattt actgagttgg
    21781 ctgaaaatga agtcttggta aaaattgcct attctgatgt caattataaa gatgcattgg
    21841 caatgagcga gtctggtcag gtgatacgaa catatccgat gactccaggg attgatttat
    21901 ccggaactgt cgtccaaagt aacaatccac gtttttcaaa agaagatctt gtacttgcta
    21961 ctggttttgg gcttggcgtc acccatccag gcggttatag ccaatatcaa aaagtaccag
    22021 gagactggtt agttcctcta ccaaaaaata tgacgctgcg tcaagcgatg atccttggta
    22081 cagcagggtt tactgctatg ttgtgtgtga acgctttaat ccgtcaaggt atgacctccg
    22141 ataaaaaagt tgtcgttact ggtgcttctg gcggtgtagg cagtacagcg atagccctgt
    22201 tacataaact aggattccag tcaatcatta ccttttccag aaaagaagag tctgtcacat
    22261 ggctaaaaag tctaggtgct ttgcaagtcg tacgacctga tgaatttctg ccagagacaa
    22321 caaaagcttt aggaaaacag caaatcgact atgtcatcga cacagtaggt ggcgagcagc
    22381 taagcagtct tcttccattg atctcttata acggtgcagt agctttatgt ggcaatgctg
    22441 ggggaatcaa actaaacgcg actgtcttgc cttttattct gcgaaacatc cagttgattg
    22501 gaattgattc tgtcaatgta ccaatagatc aacgtctttc tttatggcaa cagatggcgg
    22561 atttgcagat tgcagatgaa ctggtcgtcc aagagattac tctggatcaa ttgcctgaaa
    22621 cagcatctaa actacttgct ggaacacacc aaggaagaac actagtaaat gtaggtgacc
    22681 ataaatgaag attctaatca cagctggagg aacgactgaa aaaatcgatc aagtacgtgc
    22741 tattacaaat cattcgacag gacgattagg gcaagcatta gctgattatt tagctgccaa
    22801 tccggatacc acaatcgatt acgtgactac gaaacaagca ctgaaaccga aaagacactc
    22861 caatataacg atttatacga tcgaatctgc actggactta tttttacaat tagaagaact
    22921 aacgaaaaaa gaacactacg atgcgattat ccacagtatg gcagtcagtg attttactcc
    22981 tgcttttagt ttttcagaag aacagctggc aaaaaaccta cccacttctt caacgcaaga
    23041 agaactggac aattggtttg cggaaaatga acagacagat accactgttt caaagatctc
    23101 ttctaacacc gagcatcttg tgctcgtttt gaagaaaaca ccaaaaatca tttcttatct
    23161 acgtgaatgg cagccaaaag caaaaatcat tggtttcaaa ctattagtag atgtaccaaa
    23221 ggaatctctt ctggcagtcg caaaaaatag tctgataaat aacaaaactg attttatttt
    23281 cgcaaatgat ttgacggaaa tccatggcga gacacaccac ggctatctct tatctaaaga
    23341 tggaacagtg gaagaagctc aaagtaaatc tgagattgct gcattgatta ctgagaaaat
    23401 tagactggag gaatcaaaat gaaaaagatc ttactaggcg ttactggaag tatttctgct
    23461 tacaaaagcg ctgatcttac aagtcaattc gtcaaattag gtgcccaggt agatgtgatt
    23521 atgtctaaaa gcagcacaca ttttatcacg ccattaacct tgcaatctct gtcgaagcgc
    23581 gcagtccata cagacgtcat gcaagaatca aatccgcagg taatcaatca tatcgaacta
    23641 gcaaaacaag ctgacttgtt tctcatcgcg cctgctacag caaacattat cggcaagcta
    23701 gcaaacggca ttgcagacga tcttctttca acagtcgcaa tggcgcttcc agtgggaaca
    23761 ccaaaattga ttgcccctgc catgaacacg aatatgtatc aaaacccgat cactcagcgt
    23821 aatctggaaa cattgagatc tgtcggctat caggaaattg aaccaagaga atctctttta
    23881 gcttgtggag attttggaaa aggtgcctta gctgatattc agacgatcat cgaacaggtc
    23941 atgctgactc ttaatgaaag aaatgcttag aaaaaaggag tacttatgaa aaatacaaaa
    24001 aactttacat taactgcgat gtttttagct attttgattt tattagctgt tacgccttta
    24061 ggttttatcc ctatcggtcc aatcaatgca acaaccatgc atattcctgt aatcattgct
    24121 tcgatcgttt taggaccaag actaggtgct tttcttggcg gtacatttgg actgatcagc
    24181 atgatccgta gcacagttat ccaaacacct ttgtcttttg tcttttctcc gtttatccca
    24241 gtcatcggaa cagaacatgg aagctggaaa gccttgctga tcgcgtttat tcctcgcatc
    24301 ttgattgggg tggttcctta tttcgtttat aaaggtctgc gcaaactaat gaaacaaaaa
    24361 gcagattccg tttctttatt cttagctgga ctttccggtt cattgaccaa tacgatttta
    24421 gtgatgaata tgatctactt tttattccag caagattatg ccaaagtcat cggaacaaat
    24481 ttaaatgctg tttattcagc tattctagcg gttattttta caagtggtat acctgaagga
    24541 atcgtcgcag gtcttgcgac tgcagcagta tgcaatgtct tgttaagata cttcaagcac
    24601 ccttctctta ctaactgata gtcatcgtag ccgatctctg atttttttct cttttggtgt
    24661 tactattgaa tggaaagatt tgacgtcgaa aggatgatcc aaatgaaaat ttttgttgta
    24721 ggtgcaaacg gacaaattgg ccgacacctg ataaaagatt tagctccaag ttcccatgaa
    24781 atttttgcag gggtgagaga tgttgcttcc caaacattag taaaaaaaga gaatgtttcg
    24841 tatgtctctt ttgatttaac ttggtccgtg gaaaaaatga ccgaagcatt caaaggcatc
    24901 gatgtattga tttttgctgc cggctctcaa ggaaagaatc ttctgcaagt agacttggat
    24961 ggcgcaatca aaacagtgat cgcagctgaa aatgcgcatg tctctcgcta tctgatggtc
    25021 agtgctgttt atgcagatga accagcaaaa tggcctgagt ccatgacaga ttattatatt
    25081 actaaacatt atgcagatga atggctgaaa cgaactaact tagattttgt cattcttcaa
    25141 ccagtcacat tgactaacga tgaagaagtt acttccattc aattaactaa gcctaatgag
    25201 aaggcatcaa aaacgatcac tcgctctaca gttgctgctg tacttgccgc tctagtggaa
    25261 gaaacggata tttctcgtac gactctagta ctttctgaag gtagcaaaga gttgaataca
    25321 gcattccaag aatgggcaaa ggaggaatag atccatgcgt gccatttcaa tcaagcatcc
    25381 tggttctgta gatgtattag aacaaattga agtgcctgtt cctaaaccaa aacaaggcga
    25441 attactgatc aaagtccata cagcagctgt caatcgaaca gatattatga aaagagaatc
    25501 agtctctttg aaacctccct accctatttt gggagtcgaa gtagctggag aggtagttga
    25561 gaataaaagt gatatctcca cttttactcc tgggacaaaa gtatacggat tagtcaatct
    25621 aggtggttat gccgaatatg ctgtgatgcc tgccgatcgt gcaatccttc tccctgatac
    25681 gctagactat gtttctgctg ctggaatctc agaagttttt ttgactgctt atcaaacact
    25741 ttactggctt ggaaaattac aagaaggcga aactgtcttg atccatgcag gagcaagtgg
    25801 tgtgggaaca gcagcgatcc aattagctag acagctttct cacgcgaaaa tcatcgttac
    25861 tgcaggatca gaagaaaaac tcgccttttg taaagaactt ggtgcagatg aattgatcaa
    25921 ctataaaaaa caagactttc aagaagaggt acgaaaaatc actgaaggtc ggggagtcga
    25981 tgttattttg gatttcatcg gcgcttctta ctgggaaaag aatttagctt ctatagccgt
    26041 tgatggacgt tgggtcttga ttggtatgct aggtgggacc atcgtgcctg agatagacct
    26101 tggtgtattg attgggaaac gtgtccaact gcttggtaca ttattgacac ctagaagtga
    26161 tcagtataaa gcagagctcg cacaaacttt tatgcaaaaa gctgggcctt ttctagaaaa
    26221 tggcaagatt cggccaatca ttgaccgtac attccaattc agcgcagtca aacaagcgca
    26281 tgaatatatg gaagcaaaca aaaacatcgg aaaaatcatt ttgaagataa ctgaataaaa
    26341 tcatgctgat caaaaaagag gctgggacag aagcgtttaa ctccaagaaa taagaagaaa
    26401 ttcacgaaaa ttgcttttca aatttttgtg aatttcagct tatttccgaa ggaatttctt
    26461 ctgcttccgc cgtttatacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc
    26521 cacgctcttt ttccgaataa acggtgttca aaagcttgca tctgcggtaa taagtccaac
    26581 caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg atgcagggca
    26641 gctttttcac aacctcttca ctaaacagtg ttcaatcagc tgaccctcgg tattagctca
    26701 aaacgctgat ttcacaacct ctttttattt acgttctatt tcattcattt tccgaagcaa
    26761 atcgagctgc ttgtatgccc gcttgtcgcc caaaaataat gatctccgca actgaatttc
    26821 caccgatccg gttgtttcca tgtaagccac ctgtgacttc accagcagca tataaccccc
    26881 taattggctg gttttcttta tctaatactt cagtgttcgt attgattttc acaccgccca
    26941 tcgtgtaatg gattcctggt gcgattttga tcgcataata attcggagac gataagtcat
    27001 gttccatggc tgtggcacgg ttgaattgag catctttttt ctccgcaaca tccgtattcc
    27061 atccatcaac tgtttttgct agtgtttctt ttggtaaatc gattttttct gctaattctt
    27121 ccattgtctt tccagttgtg acatatcctt tttggtcata aaattcaata gctgtggcac
    27181 gatcacgcac gccttggtca aaaatcaaat aagctgattt ttcaggcaat gctgtaattg
    27241 ctgcagatac tttatccctt gtatccattt cattgacaaa tcttttcccc tcttgatcga
    27301 ccaagattgc tccttctccg cgaacaacct caccaatcaa gaccccctca tcctgctgca
    27361 cagttggatg aatttgtatt ttatccatat caacggtttg tccacctaat ttttcaatca
    27421 tggtgatacc atctccagtt gatccttctt gatttgtcgt aatataatca gctaaatcgg
    27481 gacgatattt ttcaataaat tctttgctgg caccaaaacc gcctgtggta actacgattg
    27541 cttttccggt gacctctttt ggttcttctc cttgaacatg tacagtcact tcattgaccg
    27601 tttccccatt tttcttaatg tctgtcacat ctgcgttgac aaaaataggg atttcccgct
    27661 catggacgtt attcaataac ccctccacta agtatccacc aatagccgag ccatcagacg
    27721 gacgatgtgt cctcttttca ctcatccctc cagtgatcgt taagttgtcc agtttgatac
    27781 cgttttgatc tagccaatcg accgcatcag cagagtgatc gacaaagtat cgaagcattt
    27841 ctttatcatt cgttcctcca ccacctttta gcgtttcttc atagaaggca tcattcgagt
    27901 ctttgattcc ttcttctttt tgaaattttg tttctgaagc attcataccg cttgatgatt
    27961 tagatgtgtt tcctccggca acaggcatct tttccaaaat tactggattc aacccagcat
    28021 cttttgcttc gatagctgct gccatccctg caccaccagc tccaataatc acgacatcat
    28081 aagattcttt catttctttt ggatctgtat atacttgttc agaagcgcct gatgatactt
    28141 cggcagattc cttcgtacta gaacttgtct gcgctgtatt tttctgatct gcgccgcatg
    28201 cgctcatagc aagaagtgca acggctgtta ataaaccagc aactaatttt ttcatttgta
    28261 tccccctttt ctccttatac tatattaagt gatttttttc acaatatcaa ctctgttttt
    28321 ctttaagtca tatagctttg gtaaacacac gcttcttagt ataattaagg atatacatga
    28381 aggagttatt ttatgaatca tttgaaaacc ttttttacga acagtacaaa aactgcaggt
    28441 agaatcacaa acggaatcta tattttatta cgtatcatcg tcgttattat gctagtggat
    28501 caggtctgga accaacactg gggaaataca atgcttttat tactgacatt aattttattt
    28561 gaaatccctt attttctgga aaagatatta aagatacaga ttccaaatat gctagaaatc
    28621 attttgttat cttttatttt tagttcaaca gttttaggag agttaagtga tttttatggc
    28681 tatttttcaa tgtgggatac ttttctacat gcattcagcg gatttttagc tgggggtgtc
    28741 ggcttttccc ttgtttattt gctcaataaa aatacaaaaa ccatgaactt gactccttta
    28801 ttactagcag ttgtttcctt ctgtttttcg atgacagttg gtgttatgtg ggagtttgtg
    28861 gaattttcag cagataaatt actgcaattc gatatgcaaa aagatacgat catccagcaa
    28921 atcaatagtg tggagttaag taaagacgga aacactgtct atcatgttcc agatatcact
    28981 cggacaatca ttgaaagtca aaatgcagat ggtaaaacag ataaaacaat catccaaaat
    29041 ggttacttag acgtgggaat catggatact atgaaggatt tatttgttaa ttttattgga
    29101 gcagttgttt tcagtgtatt tggttacctc tatagtcgaa acgattacca aaaatttacg
    29161 ttcatcaaga attttattcc taaaaaaatc aaacgataaa acatgctaaa aaacaagaga
    29221 aggggcagtg acaatacttt tgtcactgcc ccttcttttt tgaatgtatg gcatgccatt
    29281 agttatccga tagtattaac tcaaaaaagt aagatatgag gaatgactat ttcgtttttt
    29341 atcccaatac tgaggactta tttcaccatt tcgttattta tctaatttac ttaaaacctg
    29401 cataactttt tcataatctg gattaggtgt gccgtctaga ttttcagcaa taaaagtaaa
    29461 acccattttt ttataaagag caatcgcctg atggctccat gtctgtgtgt gaagatagat
    29521 tggtccgtcc gtatggaggg taggaaattc ttgcaagact ctggcagtca atgcttttgc
    29581 gagccctttt ccttggtaat ctggtttcac tgctaaccaa tgaaaaaccg gatacacttg
    29641 ctctcttgat ttttttcgcc aagcggtaca ggttgctact ttttctcctt tttttgtctc
    29701 tacaaaaaac atccttcttt caagttcttc aggataagga gcaaagtttt gttgaaaata
    29761 ctcaagtgct tcatctgtcg tttgaaattc cccaacggaa gtctcaatct ccgcccaagc
    29821 agattcatct tctttttgat aaaatcgaaa ttcaaaacct tccgctaaag gcgtgattgg
    29881 aacattttgt tgaagtggtc ttgtcatcca gatttctgcg taaggtaacg ttttatctaa
    29941 catttttcta tccctcctcg tctccattat gccaaataac atatgactca gctacttatt
    30001 tgactataac agatagagat ataaatcaga accagtcatc tgcgtcaaat tcatttcgat
    30061 tagcgtatac ccatgttcct gccatacctc gcatgaaatc tcctaaagca gaagaaggaa
    30121 tacctccacc aggtgcgggt gttcccggac taggaagtgg tacttgcggc tgcagatgct
    30181 catcggtgga atcaatagtt gacaagcgtt ttttcttcgt ttccttctgg tcatctgatt
    30241 ccttctgatc ttccgactgt ttagcaacaa attctttttc tgattgttgt tttattttag
    30301 gctctaaaat ttcattttcc aatccagaaa cttgtggggc caaatgagct tcttgcctta
    30361 tcgttttatt agattctgag atttttctag atattcttgc taactctagg cttcttgttt
    30421 gttttacttt ccctgttgga gaaactgcat gagccatttg ttgttttttt tctgacaagc
    30481 tttcttcttg tcttttttgt tctttttgat ctatctgttt gtttaatggt aaaacatgta
    30541 atagtgactg agcatggagt tcttggagtg aatacgggtc atgataacta tacataatcg
    30601 taaaatcacc tgtttgtttg gtcaaataac catcctccaa tggtacttca ttgattgtaa
    30661 ccgatacatc ctttaacggt acgggtttac ctaaggaatt tgttgctgat tggatcaact
    30721 cttctttgtt tattttttcg tttggatgaa caataagatc atgaccttct aatgaacgat
    30781 tgtctggtat ataaacataa gtaaaggtaa tttcccctcc tgattgcttc ctattattca
    30841 tcagttctga gtgaagtact tcaaggatct ccgaccagtg actttttctg tctaatccgt
    30901 ttgcttgcat ccaatcagaa atagaagtga ctttcccatt ttgttcataa gtcgataaat
    30961 ctatatttgg cactcgatca gaaaattcag caggtaggcg caaatcagga atacgattta
    31021 cactactcat atgtgtttct gcttgaagtt ctttatttgt ttgactatct ataaaatgag
    31081 tgatgacagt cgatgaagca gcagatgctt ctacataaac tacagctgtt gtttctgcca
    31141 catttgatgt tttcgagtct atataagtta atacaacggg atattctccc ggagttgtag
    31201 tatctactgt cccgttcatt ttgatttctt ctttatttcc tgaagttcca tcgctttttg
    31261 ttacatattc aacaagatct ttaggggcgt aactctctcc tgttcttaag tgaagatctt
    31321 ttcctttgat agctgtttga tcagcacgaa catggatcac agcagatgca gtcattgttt
    31381 ttaaagcaat tggatcaaaa taggacaagg aaatagtaga tgttcctgta ttagtcgtat
    31441 ccacgatatt cccattgttt gaaattactt tggacatatc tccttgtgtt ccatctgcgt
    31501 tcttcagcct ttctaccaga tcagatatcg aaacttttgt ccctgccaga acatcgattt
    31561 tctttctttc aaactgtgaa taatcttttt catatacata agaaacttgt gcgcttccac
    31621 cgtcctttat atctgtcaaa gcatgattca aaatatcagt gatctgtttt aactgatcac
    31681 cttttgtagg atcagtcata tcggaaaaga tagaaagcgg cacactgctt ccggtttccg
    31741 atgtccataa tgttttgtta ggaattaacc agtagtggtt tatcgttgga ggataactga
    31801 ctttattata ggaagtcaat gcaggaagta gctgttttcc atttgcatca gtgaaagtat
    31861 gagtaatagt gataggggaa tcagtggtct cagaaacaga ggtacgtacg accttgatgg
    31921 tttgtgtcgt tgcctgaacg ggtacatgca tatagacacc tacagataaa ctgatcagca
    31981 tcgtgcgcat cataaactta acgtagtgtt tttcagaaca cgaccgttcc ttatctaaac
    32041 gacaagcgtg tttatttagt tgttttgaac gtctttttga acacatttat tcttagtcct
    32101 ccctactctt gtctaaacat gaagtagtaa ttttcataag atgatttcat tatataaata
    32161 aaatcaaaag aagatggact attaggagag tatttttttt gattttttat tgtttcaaga
    32221 aaatcggaat aataacttat gtacttttta attttgttaa gtaatatatc tagttatatc
    32281 tagttatatc tagttacctt ttctttgaaa aaaacataaa tcagctaata aatatgtgag
    32341 acaaagctgc catccataaa tactagctga ttttttcagt taaatgtcta tttttttctc
    32401 tgatgatggt atttccaaaa tattcccagt catctggagt cggtgg
//
LOCUS       DAZKOR010000024        31896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000024 DAZKOR010000000
VERSION     DAZKOR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.024"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..601)
                     /locus_tag="ACG8ZM_001035"
     CDS             complement(26..601)
                     /locus_tag="ACG8ZM_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFQ0492012.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="ACG8ZM_001036"
     CDS             complement(606..1580)
                     /locus_tag="ACG8ZM_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0492013.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="ACG8ZM_001037"
     CDS             1863..2831
                     /locus_tag="ACG8ZM_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0492014.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="ACG8ZM_001038"
     CDS             2928..3221
                     /locus_tag="ACG8ZM_001038"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0492015.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="ACG8ZM_001039"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="ACG8ZM_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0492016.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="ACG8ZM_001040"
     CDS             3678..4433
                     /locus_tag="ACG8ZM_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0492017.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="ACG8ZM_001041"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="ACG8ZM_001041"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0492018.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="ACG8ZM_001042"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="ACG8ZM_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0492019.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="ACG8ZM_001043"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="ACG8ZM_001043"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0492020.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="ACG8ZM_001044"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="ACG8ZM_001044"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0492021.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="ACG8ZM_001045"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="ACG8ZM_001045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0492022.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="ACG8ZM_001046"
     CDS             9170..9712
                     /locus_tag="ACG8ZM_001046"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0492023.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /gene="atpA"
                     /locus_tag="ACG8ZM_001047"
     CDS             9735..11291
                     /gene="atpA"
                     /locus_tag="ACG8ZM_001047"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0492024.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="ACG8ZM_001048"
     CDS             11305..12207
                     /locus_tag="ACG8ZM_001048"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0492025.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="ACG8ZM_001049"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="ACG8ZM_001049"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0492026.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLTGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="ACG8ZM_001050"
     CDS             13650..14072
                     /locus_tag="ACG8ZM_001050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0492027.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="ACG8ZM_001051"
     CDS             14541..14771
                     /locus_tag="ACG8ZM_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0492028.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="ACG8ZM_001052"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="ACG8ZM_001052"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0492029.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="ACG8ZM_001053"
     CDS             16182..16358
                     /locus_tag="ACG8ZM_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0492030.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="ACG8ZM_001054"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="ACG8ZM_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0492031.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="ACG8ZM_001055"
     CDS             16853..17320
                     /locus_tag="ACG8ZM_001055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0492032.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="ACG8ZM_001056"
     CDS             17405..18289
                     /locus_tag="ACG8ZM_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0492033.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="ACG8ZM_001057"
     CDS             18403..18639
                     /locus_tag="ACG8ZM_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0492034.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="ACG8ZM_001058"
     tRNA            18727..18807
                     /locus_tag="ACG8ZM_001058"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="ACG8ZM_001059"
     tRNA            18814..18885
                     /locus_tag="ACG8ZM_001059"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="ACG8ZM_001060"
     CDS             19011..19181
                     /locus_tag="ACG8ZM_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492035.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="ACG8ZM_001061"
     CDS             19401..20108
                     /locus_tag="ACG8ZM_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0492036.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="ACG8ZM_001062"
     CDS             complement(20167..20811)
                     /locus_tag="ACG8ZM_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0492037.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="ACG8ZM_001063"
     CDS             20938..21225
                     /locus_tag="ACG8ZM_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492038.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="ACG8ZM_001064"
     CDS             21333..21653
                     /locus_tag="ACG8ZM_001064"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0492039.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21734..23047
                     /locus_tag="ACG8ZM_001065"
                     /pseudo
     CDS             21734..23047
                     /locus_tag="ACG8ZM_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            23044..23763
                     /locus_tag="ACG8ZM_001066"
     CDS             23044..23763
                     /locus_tag="ACG8ZM_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0492040.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            23891..24442
                     /gene="raiA"
                     /locus_tag="ACG8ZM_001067"
     CDS             23891..24442
                     /gene="raiA"
                     /locus_tag="ACG8ZM_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0492041.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            24664..27198
                     /gene="secA"
                     /locus_tag="ACG8ZM_001068"
     CDS             24664..27198
                     /gene="secA"
                     /locus_tag="ACG8ZM_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0492042.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            27324..28425
                     /gene="prfB"
                     /locus_tag="ACG8ZM_001069"
     CDS             join(27324..27395,27397..28425)
                     /gene="prfB"
                     /locus_tag="ACG8ZM_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0492043.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            28530..29216
                     /gene="ftsE"
                     /locus_tag="ACG8ZM_001070"
     CDS             28530..29216
                     /gene="ftsE"
                     /locus_tag="ACG8ZM_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0492044.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            29209..30093
                     /gene="ftsX"
                     /locus_tag="ACG8ZM_001071"
     CDS             29209..30093
                     /gene="ftsX"
                     /locus_tag="ACG8ZM_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0492045.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            30245..31693
                     /gene="cls"
                     /locus_tag="ACG8ZM_001072"
     CDS             30245..31693
                     /gene="cls"
                     /locus_tag="ACG8ZM_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0492046.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT        10608 a         5564 c         6908 g         8816 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtctta caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa agattacccg gcatatattt attgtccaat acctttatct
    23461 gaaaaaaggt ggctagaaag aggattcaat caagtaagcg agatgttgaa agcagcaaaa
    23521 attccttttc agatgctgct caaaagagag caacatctgc ttgctcaagc aaaaaacacg
    23581 cgaaaggaaa gattggagat gcctcagcct tttgagcttg ccgtacctct ttctaagata
    23641 caacataaaa aaatattgat tattgacgat gtctatacaa caggacagac gctttttcat
    23701 gcagcagatt gcttgcagaa cgcacagccg aaaatcatcc gaaccatttc tttggcaaga
    23761 tgaatcagat gtggaaattt gatagcgatt taatttgcaa ataacctaga gttcgatata
    23821 atagaattaa gaagagggaa agagtggtcc ttctccttct tgattgtggt agacgaaagg
    23881 ggtaattttt atgtttagat ataatgtacg cggcgaaaat atcgaagtaa ctgaagcgat
    23941 tcgcgactac gttgagaaaa aagtaggcaa actagaacgc tatttcagtg acgcacccga
    24001 tgcaacggcg catgtgaact taaaagttta tacagaaaaa acagcaaaag tggaagtaac
    24061 gatcccatta ccttatcttg tattacgtgc cgaagaaact tctccagatt tatacgcaag
    24121 tatcgatttg gttgttgaca aattggaacg ccaaatccgc aaattcaaaa caaaaattaa
    24181 tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct gctgctttat tcaatgaaga
    24241 ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt acgaaacgcc tttcattgaa
    24301 accaatggat agtgaagaag cagtcttaca aatgaacatg ctgggacata acttctttat
    24361 ttttgaagat gcagaaacaa atggtacaag tatcgtttac cgccgtaaag atggaaaata
    24421 cggactgatt gaaacagact gatcagcctt acttaaataa caacgaaaac ttgcagcagc
    24481 tactgacagc tgccgcaagt ttttttgagt ttcctcctct tttagatgaa atatgaataa
    24541 attatgaagt tcttacttta atacgcttaa gatatttcaa ttatctataa ctaatgataa
    24601 aatgaaaagt aaagtctaca tagtctggat aatacacaga aggaaaagga gattacacaa
    24661 cgcatggcca atttgattcg ttcacttatc gaaaacgata agaaagaact aaaacgttta
    24721 gatagcattg caaaaaaagt tgaagcatac gctgaccaaa tggcagcatt aactgatgaa
    24781 caattacagg caaaaactgc tgaattcaag ggcagatacc aaaaaggaga aacattagat
    24841 caattacttc cggaagcttt tgcagtcgtc cgagaagcag caaaacgtgt attaggttta
    24901 tatccatacc acgtacaatt gatgggtgga atcgtgcttc acgatggaaa tatccctgaa
    24961 atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc cagtatatct aaatgctctg
    25021 actggcgaag gggtacacgt agttaccgtc aatgaatatt tagcaacccg tgactcgacg
    25081 gaaatgggcg aactttataa tttcttagga ttaacagtgg ggttgaatat caactcaaaa
    25141 tcttcggatg aaaaacgcga ggcttataat tgtgatatca cttatagtac gaataatgaa
    25201 ttaggatttg actacttgag agacaatatg gtcgtttacc gccatcaaat ggtacaacgt
    25261 ccgctgaatt atgccattgt cgatgaagtt gactcaatct tgattgatga agcgagaacg
    25321 ccgctgatca tttcagggca agcagaaaaa tccacagctt tatatacaag agcagacaac
    25381 ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg atgtacaatc aaaaacaatc
    25441 agtttaaccg aagcagggat cgaaaaagca gaagaaacat tcggtttgga taacttatac
    25501 gacattgaaa atactgcatt gacccatcac ttggatcaag cattgagagc caactatatc
    25561 atgcttttag atatcgatta tgttgtccaa gacggaaaag tactgattgt tgaccagttc
    25621 actggtcgga tcatggatgg acgccgttat tcagatggac ttcaccaagc aatcgaagct
    25681 aaagaaggcg tagagatcga agacgaaacg aaaaccatgg cgacgatcac tttccagaac
    25741 tatttccgta tgtacaagaa acttgcggga atgactggta cagctaaaac agaagaagaa
    25801 gaattcagag aaatttataa catccaagta taccagattc cgacaaatcg tccggttatc
    25861 cgtgatgatc gtccagattt gctttaccca acattacaaa gcaagttcca cgctgtcgtg
    25921 caagatatca aagatcgcta ccgcaaaggt caacctgtgt tagtcggaac tgttgcggtt
    25981 gaaacatctg agttattatc agatatgctg aatcaagaac gtattcctca tgaagtgctg
    26041 aatgcgaaaa atcactttaa agaagcagag atcatcatga atgctggtca aaaaggtgct
    26101 gtgacgattg ctaccaacat ggctggtcgg ggaacagata tcaagttagg acttggggta
    26161 agagaacttg gtggtctagc agttatcggg acagaacgac atgaatctcg tcgtatcgat
    26221 aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg gtatgtcaca gttctacttg
    26281 tcattggaag atgatttgat gaaacgattc ggttcagaac gaatcaaagc tttcttagat
    26341 cggatgaaga tcggtgagga agatgctgtt atccaaagca agatgctgac gaaacaagta
    26401 gaatctgcac aaaaacgtgt agaaggaaat aactatgaca cacgtaaaaa cgtcttacaa
    26461 tacgatgatg tgatgcgtga acaacgtgaa gtaatctata aacagcgaca agaagtaatc
    26521 atggaagaaa aaagcttatc caagccacta atgaacatgg tcaaacggac aatcagtcgt
    26581 gtagtagatg cgcatacaca attagagaaa gaaaattgga atttggaaag tatcatcgat
    26641 tttgccggca gtactcttgt ccatgaagat agcattactc ttgcagatat cgaaggaaaa
    26701 acagctcagg agatcaaaga gtatctaaac gaacgagcac aagaagtatt taataccaaa
    26761 gcggctcaac taaatggtcc agagcaactg ttggaatttg aaaaagtcgt gatcttgcgt
    26821 gttgtggact ctaaatggac agaccatatt gatgcaatgg atcaattacg tcagtcaatc
    26881 ggcttacgtg cttatggaca gaacaatcca ttagtcgaat atcaaacaga aggctataag
    26941 atgtttgaag atatggtagg cgccatcgag tatgaagtca ctcgaatctt tatgaaagct
    27001 gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag gaacagcttc tcactcagaa
    27061 gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa aacagccaat ccatgtgaat
    27121 aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga aaaaatataa gaactgtcat
    27181 ggaaaaaatg caaactaatt tctgacagca ggattggtga gacaaattct ttttttaaga
    27241 attcgtctca ccttctttct gttatttgct gattctgtta gactagatat aatgaaaaaa
    27301 gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat tcgaaatctc ctgacagaaa
    27361 tgcaggaaaa ggtcacaagt ttcaggaggt ctctttgact tagatcagct ggaagaagac
    27421 atcgcggaag cagaagcaaa aatggctgaa cctggttttt gggatgacag cgaagccgca
    27481 caaaaagtga tcagcgaaaa taacgctaat aaagaaacct atgaccaatt ccattctctt
    27541 gccgaagaac tagaagaaat cgaattgatg gtggagatgc tgcaagaaga acctgatgca
    27601 gcaatgcaag aagaagcaga aacacgtatc aatgcattag atgaaaaaat gaagacgtat
    27661 gaattatcta tgctgttaga cggaccttat gatcgaaaca atgcgatcgt cgaattgcat
    27721 ccaggggctg gcggaacgga gtcccaagac tggggcagca tgcttttacg tatgtataca
    27781 cgttgggcag agcagcatgg ctatcaagta gaaacactag attatcaagc aggtgatgaa
    27841 gcagggatca agagtgtgac cctgctgatt aaaggacata acgcttttgg ttatctaaaa
    27901 tcagaaaaag gtgttcatcg ccttgtacgg atttcgcctt ttgactcagc gaaacgacgt
    27961 catacttctt tttgttcggt agatgtgatg ccagagcttg atgaaacgat cgatatagaa
    28021 atcaatccag atgatttgaa aatcgatacg taccgagcaa gtggtgcagg tggtcagcac
    28081 atcaataaaa cagaatctgc tgtacggatc actcacttgc ctacaggaac agtagtagct
    28141 agtcaggcac aacgctcaca attgaaaaac cgtgagcaag cgatggggat gttaaaagct
    28201 aagctttatc aattagaagt ggaaaagaaa gaacaagaag cagctgcact tcgaggagaa
    28261 caaaaagaaa tcggctgggg ttcacagatc cgttcttatg ttttccatcc ctattctatg
    28321 gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc aagcagttat ggacggtgac
    28381 cttgatttat ttattgacgg ctatttaaaa atgcatctac aataagaacc cattgttaaa
    28441 ttgaaataaa attgtaagat tttgcaagca tgttgaaaca ttgccatgct ataatagcta
    28501 aagactgaaa aagatatgga gagaacgcca tgattgaaat gaaggatgta atgaagaagt
    28561 actcaaatgg tacgaccgct atccgcaatc tctcgattgt catcgaacaa ggagagtttg
    28621 tctatgtggt tggtccgtct ggagccggta aatctacatt catcaaattg atgtatcgag
    28681 aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca tgatctaata gcaatcaaaa
    28741 accgcgaagt tccttatcta cgcagagaaa tcggcgtggt gttccaagac tataaattat
    28801 taccaagaaa aacagtatat gaaaatgttg cgtacgctat gcaagttatc ggcagaaaac
    28861 ctcgagatat caaaaaacga gtaatggaag tgctggacct tgttggtttg aaacacaaag
    28921 ttcgagtctt tcctagcgaa ttatccggtg gggaacagca acgtgtctct attgctcggg
    28981 caatcgtgaa tacgcctaaa gtacttatcg cggatgaacc gacaggaaat ctggatccag
    29041 aaaactcatg ggaaatcatg aaattattag atcggatcaa tgcccaagga actactgtcg
    29101 tgatggctac acataacagt acgatcgtaa atacgattcg gcaccgagta attgctatcg
    29161 aaaacggccg cattattcga gatcaagcgg aaggggaata tggatacgat gattagaacc
    29221 tttttccgcc atttattaga aagtatcaag agtttgaaac gtaacggctg gatgacagtt
    29281 gcttcagtca gtgcggtaac gatcacgttg actttagtcg gtgtattcat ggcagttatc
    29341 atgaatgcaa ctaaattagc tcaggatata gaaggaaacg ttgatgtatc tgtattcgta
    29401 gatatcggta caaaagacga agagatgaaa aagctagaaa aagaattgaa tgacttagat
    29461 catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat tgaaaaaaat ccaaaacgaa
    29521 atgggtgatt cctggaaatt gtttgaaggt gacaataacc cgttatatga tgtgtatgta
    29581 gtaagtgcta ctgatccttc ttatacaaaa caagtagcag aagaagcagc agatttgaag
    29641 aatgtttctc gttctgatta cggtggagct tcctcagatc ggattttcca aatcgctggt
    29701 gcagttcgga catggggatt agttgcagcg gcattgctgc tgttcgtagc gatgtttctc
    29761 atttcgaaca cgattcggat cacgattttg tctagacagc gtgaaattca aatcatgcgt
    29821 ttagtcggtg caaaaaatgg atatattcgc tggccgtttt tccttgaagg tggctggatc
    29881 ggtctgatcg gtgcaattct tccaattttg atcatcacat ttggttatgc atggtttttc
    29941 caattgatca atccatcatt attacgttcc cattattcat tgattcatcc acaaaatatc
    30001 gtttggaaaa tcaatctttt aatggtcggc attggtgtga tcattggttc acttggctcg
    30061 atcatttcta tgagacgttt cttgaaattc taataccaca atataaataa aatgagaaag
    30121 ttggacagtt tcgttccagc tttctttttt tattggctag gagaatagaa ggaagcggct
    30181 tttttccctt tactttaaaa aacaacttgg gtattcttaa gttggtagaa gaagaggtga
    30241 agccgtgaaa attatcgttg acaatttttt tacgatcctt ttgattatga atattttgct
    30301 ttcctttatc atcgtttttc gagaaagaaa ggaaactgct caaacatggg catggctgct
    30361 cgttttgatg ttcattccag ttgtaggatt tattttatat atttttttag gaagagggat
    30421 ttccaaagag aaaatctttg atttgaagat ccaagggaaa atcggtaaga acctggaaat
    30481 tgaagaagac aaacaggcat tgatgcgagg gctttatcct catccgccta ctggacaagt
    30541 agatgtgaaa caattgatct atatgctcac ggtatttgaa agtacgctct atacgactaa
    30601 aaatgaaatc actttattta cagatggtcg tgaaaaattc gatgcgctga ttgaagatat
    30661 caagcaggca gaagaccata tccacttcca gtattatatt tatcgcagtg atgcattagg
    30721 tgaagaagtt cgagatgcat tgacagaggc ggcaagacga ggggtaaaag tcagagtcct
    30781 tcttgatgca tggggttcca cacaagtttc ttccagcttt tttcagaatt tgaaaaaagc
    30841 aggcggagaa gtcgcatttt tctttcctct atttgttccg tatattaatc ctcggatcaa
    30901 ttatcgcaat catcgtaaaa tcgtagttat tgatggggaa atcggatata caggtgggtt
    30961 caatgttgga aatgaatatc taggattggt aaagaaattt ggttattggc gagacaatca
    31021 tttgcgtatt tacggagaag cggtgtatac cttgcaaaac cgttttttga tggactggaa
    31081 ttctcagcat acaaaagaag taggctattc tcccaaattt tttccgtcca ttcacactac
    31141 tggtgatata gctgttcaaa tcgtgacaag cggaccagat accgaacatg aacaaatcaa
    31201 aatgacttat ttgaaaatga tttcgttagc caaacgggag atattgatcc aaacgcctta
    31261 ttacataccg gatggatcga ttcatgaagc actgaagttg gctcttcttt caggcgtaaa
    31321 agttcatatc caaataccta acaaaccaga tcatcttctc gtttactggg caacgtattc
    31381 tttcgctgca gagctgatcg agtatggagc aaggatcgaa acatatgaga atggatttat
    31441 tcatgcaaag acgatgatta tcgatggtgg cattgtttca gttggctcag caaatatcga
    31501 tgttcgttca tttcgcttgg actttgaggt caatacactg gtttatgatg aacgaatggc
    31561 ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt tctacgcatc tcacaaaaga
    31621 aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa ggattagcaa gattgatttc
    31681 gccgctcctt taaaacataa aaaggacatg agaaccatca acagatggag atctcgtgtc
    31741 cttttttaac ggggccattt ctgagagtac gtcagaaatg gagaaagcgt ctcgcatgag
    31801 cgaaattaac tttggggtat ctcttgtttt ttggggaaag caagaaataa gctaatatat
    31861 gctattaaaa gagacacatt tcaaagccag tatagc
//
LOCUS       DAZKOR010000025        30936 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000025 DAZKOR010000000
VERSION     DAZKOR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.025"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..420
                     /locus_tag="ACG8ZM_001073"
     CDS             <1..420
                     /locus_tag="ACG8ZM_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0492047.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="ACG8ZM_001074"
     CDS             complement(504..1226)
                     /locus_tag="ACG8ZM_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0492048.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="ACG8ZM_001075"
     CDS             complement(1216..1953)
                     /locus_tag="ACG8ZM_001075"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492049.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2958)
                     /locus_tag="ACG8ZM_001076"
                     /pseudo
     CDS             complement(1965..2958)
                     /locus_tag="ACG8ZM_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(2973..3716)
                     /locus_tag="ACG8ZM_001077"
     CDS             complement(2973..3716)
                     /locus_tag="ACG8ZM_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0492050.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3790..3894)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3957..5279)
                     /locus_tag="ACG8ZM_001078"
     CDS             complement(3957..5279)
                     /locus_tag="ACG8ZM_001078"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0492051.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5266..6231)
                     /locus_tag="ACG8ZM_001079"
     CDS             complement(5266..6231)
                     /locus_tag="ACG8ZM_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492052.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6228..7766)
                     /locus_tag="ACG8ZM_001080"
     CDS             complement(6228..7766)
                     /locus_tag="ACG8ZM_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0492053.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8182..8469)
                     /locus_tag="ACG8ZM_001081"
     CDS             complement(8182..8469)
                     /locus_tag="ACG8ZM_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492054.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8603..9271
                     /locus_tag="ACG8ZM_001082"
     CDS             8603..9271
                     /locus_tag="ACG8ZM_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0492055.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9562..10302)
                     /locus_tag="ACG8ZM_001083"
     CDS             complement(9562..10302)
                     /locus_tag="ACG8ZM_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492056.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10432..11379)
                     /locus_tag="ACG8ZM_001084"
     CDS             complement(10432..11379)
                     /locus_tag="ACG8ZM_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0492057.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11508..12272)
                     /locus_tag="ACG8ZM_001085"
     CDS             complement(11508..12272)
                     /locus_tag="ACG8ZM_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0492058.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12276..12665)
                     /locus_tag="ACG8ZM_001086"
     CDS             complement(12276..12665)
                     /locus_tag="ACG8ZM_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492059.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12800..13342
                     /locus_tag="ACG8ZM_001087"
     CDS             12800..13342
                     /locus_tag="ACG8ZM_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0492060.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13490..14122
                     /locus_tag="ACG8ZM_001088"
     CDS             13490..14122
                     /locus_tag="ACG8ZM_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492061.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14256..14381)
                     /locus_tag="ACG8ZM_001089"
     CDS             complement(14256..14381)
                     /locus_tag="ACG8ZM_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0492062.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14515..15408)
                     /locus_tag="ACG8ZM_001090"
     CDS             complement(14515..15408)
                     /locus_tag="ACG8ZM_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0492063.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15590..16516)
                     /locus_tag="ACG8ZM_001091"
     CDS             complement(15590..16516)
                     /locus_tag="ACG8ZM_001091"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0492064.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16601..17362)
                     /gene="pflA"
                     /locus_tag="ACG8ZM_001092"
     CDS             complement(16601..17362)
                     /gene="pflA"
                     /locus_tag="ACG8ZM_001092"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0492065.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17606..19837)
                     /gene="pflB"
                     /locus_tag="ACG8ZM_001093"
     CDS             complement(17606..19837)
                     /gene="pflB"
                     /locus_tag="ACG8ZM_001093"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0492066.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20114..22564)
                     /gene="parC"
                     /locus_tag="ACG8ZM_001094"
     CDS             complement(20114..22564)
                     /gene="parC"
                     /locus_tag="ACG8ZM_001094"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0492067.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22576..24621)
                     /gene="parE"
                     /locus_tag="ACG8ZM_001095"
     CDS             complement(22576..24621)
                     /gene="parE"
                     /locus_tag="ACG8ZM_001095"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0492068.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24787..25221)
                     /locus_tag="ACG8ZM_001096"
     CDS             complement(24787..25221)
                     /locus_tag="ACG8ZM_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0492069.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25348..25986
                     /gene="plsY"
                     /locus_tag="ACG8ZM_001097"
     CDS             25348..25986
                     /gene="plsY"
                     /locus_tag="ACG8ZM_001097"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0492070.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26118..26993)
                     /locus_tag="ACG8ZM_001098"
     CDS             complement(26118..26993)
                     /locus_tag="ACG8ZM_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0492071.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27075..27866)
                     /gene="codY"
                     /locus_tag="ACG8ZM_001099"
     CDS             complement(27075..27866)
                     /gene="codY"
                     /locus_tag="ACG8ZM_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0492072.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27884..29284)
                     /gene="hslU"
                     /locus_tag="ACG8ZM_001100"
     CDS             complement(27884..29284)
                     /gene="hslU"
                     /locus_tag="ACG8ZM_001100"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0492073.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29298..29846)
                     /gene="hslV"
                     /locus_tag="ACG8ZM_001101"
     CDS             complement(29298..29846)
                     /gene="hslV"
                     /locus_tag="ACG8ZM_001101"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0492074.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29883..30788)
                     /gene="xerC"
                     /locus_tag="ACG8ZM_001102"
     CDS             complement(29883..30788)
                     /gene="xerC"
                     /locus_tag="ACG8ZM_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0492075.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT         8528 a         6511 c         5270 g        10627 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt ttcaaatcct ttatttcttg
     2881 tttgctactt gtttcttttg cagtgccaca agcggataaa agcaacagta gcggtaataa
     2941 aagccataat tttttcattt gatccccttc tcttaaaaaa tataaagcat acgataatac
     3001 ccttttttca ataatgaaca agcgccattc agcaataaac tcaatagaat gacacaaatg
     3061 atcgcagcaa aaactttatc gatctggtag ctgtttttta cgcgtatcat gtaataccct
     3121 aagcctgctt gagcgcttag ccattctcca acgactgctc cgctgacaca ataagtagca
     3181 gccactttta aaccggaaaa gaagccggat aaacccgctg gtattttata tattcgataa
     3241 atcttccctg tatctgcccc catcgttttc aaaaagatga tttgctcttt tgatactttc
     3301 ctcaatgtat ccgaaaaaga aactacgatc ggaaaatagc ttatcaagac aacaaggatc
     3361 acttttggaa gcataccgaa accaaaccat agggtaagta atggccctaa caccatgatt
     3421 ggcaacattt gtgaaatcac tagtaacgga taaaaagaca tttctaaaaa cttccaataa
     3481 tccattgcca aggcagtaat aaaagaaaga acgctagcta ttaccaaacc aagcactgct
     3541 tcatttaatg tcgtgacaga atgggaaagt aatacttgtg catctgccgc taatgcagaa
     3601 aatacagcag caggtccggg taaaataaag gcaggtattt tccaaactat gacaacaatc
     3661 tgccaaataa ttaaaatgaa tgccatactg actgctggat aaagattttt cttcattttg
     3721 ttacctcctt tcttctaata tgaataacaa aaaacgcttg aaatgggcac gacatagtaa
     3781 cccgcttcaa gcgtctgcta tgtttctccc ttcaccagta ttaactgaca ggttcaatgg
     3841 gtctaagttc acaacttact ctcatgtcgt ctacgacatc cccagaaaca ggtattcatt
     3901 ttttaaagtg tagcacaaga aggaaaaagc aacaagcgct ttagttatca tgctgattat
     3961 ttcaataaat aggagagatc acttgccaat gtcggatacc ccatgatata gctatccaac
     4021 tcttttttag tcaatttttg attgatgacg agtgtcaaat catcgatcaa ttcgtctgct
     4081 tgctcgctaa tcactgcagc accaatcaaa taattttgct ggtcaaaggt caattcagcc
     4141 ttagcaactg gttcattgac acgatgatag gtgaaccaac tggtaagatc catctctacc
     4201 acttgatctg acgaagaggc gtgacttttt gtaactccta cttcagcaag tttaggactg
     4261 gcgtagacga tcgtaggtat aatcggatat ttgataggtt cgcttgttgc atccgttatc
     4321 cgcttagcta cataatttcc ttcaaaagtg gcgactggtg tcaattttgg ggttttcctt
     4381 gaaacaatat ctccacacgc aaaaattttt ggattgctcg tttgcagata atcattgact
     4441 gcaattccgt gtttatcaaa gacgatattc gcttgctcaa gtgctaaaga ttctgtattc
     4501 ggctgtctcc cagtcgcaca aaaaatcata tctgctacta attccgtctc ttttccgact
     4561 aaacgatagc gagtaccttc tgaaatgatc ttttgggttt caacaccaaa agcaaactga
     4621 atgccagatg cttccatttg atggacggct tcctctacca aagacgcctc aaattctttt
     4681 aatggccgtt gattatgatg gacaatggtt actttgctcc ctgcagcatt ggcaatcgta
     4741 gctagctcaa aagcaatata accaccaccg ataaaaatga tttctttagg cagtacaggc
     4801 aatgataaaa agtctgcact agttttcaag tattcctttc cctccactgg taagatcgta
     4861 ggtctttgcc cggttgccaa aactaagtaa tccgcatgga agacttcttc gttgacttca
     4921 atagatgaat catccaaaaa acgagcggtt cctgaaagat gatcgatttc agcttctgct
     4981 aattgttttt ttcggctttc tgggactgga tccgtaaatg ttcgtttgaa tttttgaagt
     5041 tcttcccagt tggcagttgg gatctcgtta aaaccttttc cactcaattg ttttacccga
     5101 ttacgtgcct ctactgcact taacaagact tttttaggat cacatccacg gttaggacaa
     5161 gtcccgcccc atagatcttc ttcgaccact agtactgttt tacctgcttc tttcaatcca
     5221 taagcagcac ttaagccgct cacacctgaa ccgatgatga ttgcatcata tgttttcatt
     5281 tccttcctcc tctcgactta aaaaagcaaa gatttcttta cttgtcagcg acccaatact
     5341 tctttgcttt tctactatca caatgcttgg atacaccgaa agaagcgaat atacttcttt
     5401 cataggcgta tcaaagcaaa cttgttgacg agcattttct ggtatttcat ttaagaaata
     5461 tccattgacg actaaatctt ctactcgcca ctctttcgtc tgtttcggtt catcaaagaa
     5521 atgccgaacg tagtcgtttt taggattcat caataactgc tctggtgtgt cacattggat
     5581 aatttctcca tccttcataa atgcaatacg atcacctagc ttgattgctt cttccatatt
     5641 gtgcgtaacg aaaacgatgg tcgtgcccaa ttcttcgtgt aaggacaaaa ccaattcttg
     5701 taatgagttt cttgaaatcg gatctaatgc gctaaatggc tcgtccatca aaatcacatc
     5761 tgggcttgca gcaatagctc ggatgatccc gatccgttgc tgttctcctc cagacaactc
     5821 tctaggcata cgatctcgat aaatatcagg agataagcca actttttgaa gaagttcatc
     5881 tacacggtct gctcttttct gcttttccca acctaacatt tcagggatga cttcaatatt
     5941 ttgcttgact gtcatggtcg ggaacaaagc gatttgctgc agcacatatc ccatattcca
     6001 ccgcattttc tgcagattat attcatttaa cgattttcct ttgaagtaga tgtctcctgc
     6061 tgatggttta gataatccat tgatcatttt caaagtagtt gtttttccac taccggaagg
     6121 accaaccaaa acaaagattt ctcctttttt gattgccaga tttaggtcat cgatgacata
     6181 ctttccatca tacttttttt gcacatgttt gaactcgatt aatttagtca ctgatcttcc
     6241 tcctttacta atccttcatt tttcaggaag attcgtgcta cttctgtcgg ctttttcttt
     6301 tcgacattga cttggtaatt catatcaatc atttgatcct ctgttatttt tccgcttaat
     6361 ttattcagtg ctttaacgat ttcaggatgt tctttagcaa aatctttttt catcagcggt
     6421 gcgccttgat aagctgggaa taaccctcga tcatcttcta gagtcactag atcgtactcc
     6481 ttgagctggc tatctgtgga ataggcatcc acaatattga catctccatt attgattgct
     6541 tggtaacgca aacttggctc catgctttgt acacttgaaa aattcaagcc atatgcttct
     6601 tggattcctt tatatccatc tgaacgatca ataaattcca gtgtgaatcc cgctttgatc
     6661 tgatcagaga tagctgccaa atcggaaatc tttttaagtt gatgttcgtt cgcaaaagat
     6721 ctcttgactg ccaaggcata cgtattttgg taagccattg gttttaataa tgtcatatcg
     6781 aattgcttat tcaataattc ttctgcttcg tcatatgttt tttcttttgt caaatcagca
     6841 cttttcattg cttcaggtac cttcaccaag ctttctagga cagtacctgt gaactcagga
     6901 tagatatcga tttgaccttg atccagtgca ttgaacaaaa aactcgtctt gccaaaattc
     6961 ggttttaatt caacttctgt ttttgtgttg tcttcaatca attctttata catttggatc
     7021 aggatttcag gttccgctcc taattttcca gcaatcgtga tggtttcatt tttaaacaag
     7081 gcagactgat ataaagaaac gccgccaaat aaacacacca ctgctgctaa agtaatgatg
     7141 gaatatcgag gtttcttatg ctgcaaccag cgaatcagtc cgctgaatat aattgcaagc
     7201 aaggcagaac tgatcgcacc aatcaatgtc aaaacaggtg tatttcggtc aatacctaac
     7261 aaaataaatg tccctaatcc acctgctccg atcaatgctg ccaaagtagc cgtaccgatg
     7321 attaaaacta aggcagttcg gatacctgag ataatgacag gtaatgcgat gggaagctct
     7381 actttcagca agcgtctcat tctggacata ccaaatgcta ccgctgcttc ttcaatagac
     7441 gggtcaattt cagctagacc aatatacgtg ttttggaaaa tcggaagcaa agcgtaaata
     7501 acaagtgcaa tcaatgctgg aacagttccg atccctacaa atgggataag taaacctaat
     7561 aaggcaagtg aaggaatcgt ttggagcaca cccgtgatct gcaataagac ttctgccatt
     7621 tttttatgat tggctgccca aatcgctaaa ggaatagcaa tcagtgctgc aatcagcaaa
     7681 gaaatgagag aaatggacag atgttgaaat gcagcttcca gcaactcgcc ttttctttca
     7741 cttaatgttt gtataaaatt attcatgcga ccttcctttc tgttattctt taagtataaa
     7801 catttctttc ctttttccca actaacttgt cttgccttaa ccttacagca cgaacaaaaa
     7861 aaagaaaaaa atacctctaa atatgctgac aaaaaaagaa tccaagcaac aaagtcggat
     7921 caactttttg gttctcgttt tttcactacg aataccataa aaatctatac gaaattgtca
     7981 cttggaatca gctatatctt ttttacacac acaactttta ttttaataaa aagaaacagc
     8041 caataaagta gaaatctcgt gggggagaac acctttattg gcctgttcaa cgaatattat
     8101 actgtatcat gacacttttt tgccaaaaat gtaaaaaatg gttgttttga catcataaac
     8161 gtcttgtttt cttattttta cttatatcac cactttattt tctatctctt tcactccata
     8221 aagaggaatg agttcttctt ttgactgata ataaacagct ttctcacggt tcttcatcaa
     8281 aaaaagaaag atgtctttca tagcaagtgg tgctttgccg tgttcaaata aaaagataaa
     8341 ttgatttgct ttatttatct ggaccattgt cacaggcacc agtttttgtt cttgagccaa
     8401 aaaaactcct tgatactctg tttttttggg tgattttgta agaattgtca ataagtctgc
     8461 tgctcgcatt tttccactcc ttatttgata aataaaaaga aacagccaaa aataattttt
     8521 tttcattttc ctcttgaagt aagagtataa gtcagtataa taaagaagtt gctgaaaatt
     8581 ttcaaaagaa aagaggctag aaatgaaaaa attaaattta tttacagaat tgtgcggaat
     8641 cattttaggc gcaggaatct acggattagc tgtgacagga atcaatattc catctaagct
     8701 ggcagatggt ggggttactg gtattgcttt gcttttgcat aatctttttg gattcgctcc
     8761 ttctgtcact tcactgcttt ttaatttacc tttattgctt atctcattgt ttctttttgg
     8821 aaaacacgct tttctccgga cgatcgtcgg aacatttgcg ctagttttct ttttacattt
     8881 atgggagagc cttcatatac attttgctgt agatagtcta ctgatcaata gtttgatgac
     8941 aggtattctt tctggcattg gttgcggtct cgtctttcga ttcggcggaa gcacaggcgg
     9001 aacagatatc atttatcaag ccattgaaaa atattttcat attaagattg gcaaaagtct
     9061 tttcttgatt acttttggta ttttagttgt ctccttgctt tatttggatc tcacacattt
     9121 tctgtataca ttacttagct gttctattct atcttataca ttgaataaag tgaagtatct
     9181 ggaaattgga tctgttttgc cccaattgga agcaaaacag actacagaaa catttgaacc
     9241 acttgatcct cttgaacagt tagagcactg attcttattt ttcactactt tatttaattt
     9301 acagaaatat cattatgtaa actaaaatga tattattcag taaaatcttc tttataaaaa
     9361 aataaactat ttttgcaaac gatctgcgaa aatagtttgt ttttttattt tgccattatt
     9421 ttcttactta ttgggagaaa aaattcgctt aaactcaaaa aaatttgagg atacgatata
     9481 aactttcgga tagagtataa aataaggaaa cgccgcagcg tttcctttcc aactgtagcc
     9541 gaatataaat ttgtatatca cttatttaaa atagctattt tctcccaaag agtttccttg
     9601 atcatcgatc aatccttgta atgagcgttc taagatacca tggctttttt tcgatttttt
     9661 ttcttctgac tgctttgttt cctctaacgt ttttgttcga ttgaatgttc gtacagacgg
     9721 ctcaccattt cttttttctt gatacggcgt cggttctgta tcgaacaaca gataagagtc
     9781 atccgttttt ttcatcgatt cgattaaatg atcctcagaa attttgcttt ctctagggtc
     9841 tgggataatc gatgcgggta catatttagg aacaaaataa gaacgctccc gcaccttcgt
     9901 gtcaacagat gcgtttcgtg tagtatgctt ggactctgtc cttgtttcac tttgtctgac
     9961 agaacgagag gaatcaccaa aaagtttttt tcgtcctgtc gatgtgattt ctgattggta
    10021 atggccggta taatccggta attttccttt atgcttttcc aaattttcct tacgcggagc
    10081 tgacttcaat ccatttgttg cacgacgctt gcgacggatt ttctcggtgt tcgtgttcgt
    10141 gaaatgaaat tctttcatct gttcaaacgg ggactgattc tgttcagtca aaagatcttc
    10201 ttgtccgtca aataatactc gcttcttgtc gttggttaat tttacgccta actcatcatc
    10261 gtacgcagga aaacggaaat gttttcgtct gatttctttc attcaaacca tccttctcaa
    10321 aaatggaact tttctcaata atagcacaga ttcattgttt tcagtagcaa tgcgctgatt
    10381 attttaagaa aatagcgatg aatttcataa atatatatat agtaataaga cttaattcgt
    10441 tgtctctctg ataactagtt caggaaaaaa acgacgataa ccttgactcg cccctttttc
    10501 ttctattttt tttaacaaca tttctccaca tacttcaccc atacgtataa tatcttgctt
    10561 gatcgttgtc aatttaggtg aactgatttg gtctaaaaat accccatcaa atccaatcac
    10621 accaaattct tcgggcaagt gtcctccagc atttataatc cctcttactg ctcctagtgc
    10681 tagccgatct gaactacata caaaacaagt tttatctgta aaatctgccc agttttctgt
    10741 catatatctt tctacttctg tcgaatgatt tggaaaccgc aagattttac taggaagatc
    10801 aacttccttc atcacagata aataaccagt ttcccttgaa atttcaaaag gctcgtccac
    10861 ttcgataccg atataaataa tcgtttgata atctctttct aaggcatatt gcgttgcttt
    10921 tgctgtacca tattcattat cgctgtctac ataatcatat ccttcagaat tttccccaaa
    10981 caagacaact ggtttttcca aacggtcgat ccaatcgaag tctttttttc tgacacctgt
    11041 gatgatgtat ccatcacaag aaccgacgtc gaaggcatca tttgtaacta attggagaga
    11101 ataatgcgct ttaccgatcc gtttagcgat tcccatcagc aaattcatgt aatagggctc
    11161 agttgtgtcg atttcttcca gtataaataa tttgatgatc tgcgaccgat tcgtgacaag
    11221 tgcttttgca gcaatatttg gacggtaatc cagttcagcc attgctgaga aaactaactc
    11281 tttcaattcg tcagtcacct gttccggatg attgatgact cgagagaccg tcatttttga
    11341 aacattggca cgttttgcaa catctgctaa agtgggcatg ctctcttcct ttctaacatg
    11401 ttgtcattct tactatcaat tataccaaat tttcctaaca gagtttatta tagcccaaaa
    11461 taaaaaacgg aaaatcctct atcgtcatga gtgattttcc gtttcaatta tttatacttt
    11521 tttatcaaat tttttgctaa aaaagtggaa gcttcttcca ttagtggtat ttgtaccata
    11581 tgtcgctgtg tttttgagga acgaaaaaca atttcttttc cgtaagactt atgccgattt
    11641 ttttctacaa atcgttccgt ttgtatataa gggatttttt cgtcagcttg tccatgccaa
    11701 aagaacagag gacgtccagc taacttttcc ggctgcaatg acaaatcata gctcttaacc
    11761 catgaaatca aatcaaaata gtcttttggt acgacataat tatattccga tgcgcgcctt
    11821 acgatttctt ttccatattc caaaggtgca ggggaaccca tcaaacacac gcccgctact
    11881 atatgaggat gtttagccaa caaggcgcat gttgtcattc cccccatcga aaccccgcca
    11941 actgcaagct gatcatgtag aagacctctt ttttctaaat gcttgataag tgtgtcaaat
    12001 tcaaacagat ttccgtagat actatcccaa aatgtatagg atggaataga agaaaccggc
    12061 tgtttgcgct ccccatgatt catcgcatct ggcaggatca cacgaatgcc ctgctgcgca
    12121 atttttcttc cttgcgtcaa aactaactcc ttacttgtct gccatccgtg ataatagatg
    12181 acaacgggaa tacgttcctc tcttaattct ttcagaacaa cttctaacac tggaatatga
    12241 tggacagaac gtttcaatat aattggattc aattgttact cacctatttt cattaaattt
    12301 cctgatcgcc atagcaacca tgctccaaca gttgtctgga acaggttcag tgtaccaaat
    12361 aatataaatg caggactgaa tggctcggga aataagcaca gagaacaaag tcccaccact
    12421 cctaatgcta gcgaaaggag tggataaata gaaaaattca aaaaactggc aacgctaccg
    12481 aaaatattac caataaacag tatcaaaaag aaaaaaacgc ttaaaattaa accatctgtc
    12541 agcgacatct gaaaaacaat gttctctacc tcaattggct gttgaatcga atagacacct
    12601 aagtatatac tgagaaggag acttagcacg tggctggcac aaaacacacg attgaccgtt
    12661 ttcattttaa tcacctaacc tatctttata attctattat aatcatttta tcattttttt
    12721 gtatgtataa agtttcaatt tttcttcact tacgattttt tttgatattg ttgttatata
    12781 aatgaaagga gttttacaaa tgattgatcc aaaaatgttg gggatgatct tcctcattaa
    12841 ttttgcttat atcactctga acacgttacg tttcatgttg acaatgaaag gctatcgact
    12901 gattgctcca cttgtcagta tggtcgagat cacgatttat atcgtcggct tatcccttgt
    12961 tttagatcgt ttagataatc cactgaattt atttgtctat gcgttaggtt acgcagtagg
    13021 tatcagtgtc ggaattttga ttgaagacaa attggcatta ggatacatca tggtcacaac
    13081 gattttgcca gcaaatagcg cagaagataa aagtttgccg ggtatactga gagaacacgg
    13141 ttatggtgtg acacagactt ctgcaatggg gctggaaggt gagcggctag ttctcgaaat
    13201 actttctcct agaaaaagcg aacgtgaact gtatcgcctg atcaaagaaa tcgaagagcg
    13261 tgcttttatc atttcttatg agccaaaata tatttcaggt ggtttctgga ctaaaaaagt
    13321 gagaaaacga caaggaaaat aactcacaaa cttaaaaact gtctaatatc taaattaaat
    13381 tttaaatgtt agacagtttt tatttattat tttgattaaa aaacatcgac atatactcat
    13441 caatttgtta ttatacttta tatacttatc aatttaattt ggagatatta tggaaattca
    13501 acgtagaacc actaattttt tacatttagg ctcttttttt gcaactttga cgatcttatt
    13561 ttttctatgt tattcatacc ttaagacctc acatgcgcct tctagtgcat taggctggtt
    13621 gcttttggat cattctgata tcacagaagc cgaagaaaat ccgttttttt tgattttgtc
    13681 ttctctttgc ggcttactgt tttctgttta tttcggtgcc tatttttttc atcgacatcc
    13741 tggtacgtta aacgaagctt caagcgtatt gattgcattt aaaacgtttt cttttttagc
    13801 aattcttttt tattcgttta atcacaatac gcttttcttc tttatcatga atggtttagt
    13861 cgtgatttta tccattttta cttattttat tttttcactc atttttagcc aattgaaatg
    13921 tccgctcttc tttcgattag ttcctgtcac ctgttttatt tacagcttgt tttccttttt
    13981 tgtccttgct ttaatgccac tgaccaaccc ttcgacgatc caagtcgcag gaaatctgtt
    14041 ggatatgctc ttcatcgtca gtctaggtgt atttatgtgg caaagaaaaa acaaacaaaa
    14101 taaaaagcat tttgaagcct gactattacg acttcataaa tgctttttta gttttaaaca
    14161 cttcaatcta aaagaataac ccaagattgg ataaattatt aatgactcgc cattcgtttc
    14221 ttagtgattc tggattatca aatggacttc ctaatctaat cagctagttc ttcccataaa
    14281 atgccatact tgactgaatc ataatattga ttatttaaaa atctaacttt tcttatcact
    14341 acttcctttg tcattccaca tttctcacca tttttttgca ttcctttgtt tccagaccat
    14401 gtagtgaatc taataggtgg tagataggca aaatactcaa ataattcttt taaccataaa
    14461 ggaagagaaa ggctcctcct gcaagagtca gtataatttt tgcttactgc tctcttaggt
    14521 tttactcact aaaactgttc catattcttt gatataagaa aatgcatgcg gtataaattt
    14581 cttgctcagc tcagaaaaga tttgtcgttt aagaaaggcc atttcctcca ttgtcaatga
    14641 aggatccaat gttggtaatt caatctcaaa attgtttttt tcgacaatag gtaattctaa
    14701 tgtgaatgat tcttcttcct ttatagtatt cgtcacaagt agagactgat ggaaaatact
    14761 aggccggcga tttttatatt tattcttacg tatcctgtca acgataactt ctatttgatc
    14821 aaaaaagtac gattcattta cgtctccaat aagttcggct aattctttga atgccttcgt
    14881 tacaggaagc tgattcttct gtacaaaaaa gtaattagcc aaagtaagcg ggaagtgttc
    14941 tatagaggaa agtgtgataa aacggtactt ctcatttcct gcagttgtcc tcagcaattc
    15001 tgtctctcgg ctgtttctta ctccataaaa atacgcttct ttccgttcat cttcgaaaca
    15061 atacgcccaa ataacttctc ccatcgctaa tttttgttca tcaacagaga gtctcttcaa
    15121 ttgttcagct atggtttcag cagccgaagt ggcttgttgc aagtaatctt taggatcaaa
    15181 tattggaaag ttcaaatgat atcttcgttc ttccctcttg attaatcctg catcgatcaa
    15241 taaatcaaga aaatgggaaa atttattttg cgaaaattct tttttcaatt ctcttagaat
    15301 cgtttcttgt tctcgatgtt catatagata atcggtcaat gaagaaaaaa gcggatcatc
    15361 ggtcagtttt tgataaaagt tttccttatt ttttgaataa tagttcatat atgttctctt
    15421 ttctatccgt tttattttac ttaaaaggct tttacgtaaa aaggaggaag aacaagagtc
    15481 ataattgact gttattctcc tccatttctt cggcctttag tatgttctat gtttcttttt
    15541 atatcaatcg cttatccatt cattttgaat gcttcatact accatttgat tagctaaata
    15601 cagccgtcaa ttgaggtacg acttgttttt tacgtgaaac gacaccttta agcaaagcac
    15661 ggttgttggt caaagttgtt tggaaagctt cttctacttt ttcttttcct ttaccagcaa
    15721 caagtaattc tgaatcactg tctaagatgt ttgtgacaac taatacgaac agatcgtagc
    15781 catttgcttt catttcatct tccattgctg cttctagctc tgcttgtcga tccatcactt
    15841 cattaaggtc cactgtattg acttgagcga tacgaacgtt ggaactgccc attgggaaac
    15901 tttttgcatc caaatcaaga agaaccgcag ctgatttatc acttagattt gttcctgctt
    15961 tcaacatttc aagtccataa gtgtttagat cgacttcagc gattgctgca agttcttttg
    16021 cagctgcttc atcttcaggc gtacatgttg gtgatttgaa caacaatgta tctgaaatga
    16081 tagctgagag catgattcct gcgatttctt taggaattgt tacttggttt tctttatata
    16141 gttttaaaat aatcgtactt gtacatccaa ctggttctgc acgatagtac aatggattag
    16201 ctgtttcaaa gtttgcaata cgatggtggt ctactacagc taaaatattt acttcgctga
    16261 tatctgaaat actttgttgg aattcattat gatccacaag catgacatct gcattttctg
    16321 ctttagctga ctctactact cgtggtgcag atagctgaaa gtggtctaat gcgtattgtg
    16381 tttcttcatt aggtgtacct agagcaactg cttctgtttc tttacctaat tcattttgca
    16441 agtgtgaaaa cgcaatggct gccccgattg catctgtatc tggattttgg tgaccaaaaa
    16501 ctaaaatttt tgacatgtca ttcgctcctt tgtgatttct cctatttaat aatagcaaaa
    16561 aaacggaaag aagcaatact tatcttcttt ccgatgcgac ctatcgcgtt aaatattttg
    16621 tatactcttc tgtatgaagc aatcgtttag cattttcgac acgttctttt gtaggtggtt
    16681 cgatcccttc taatggatat ttcaaaccaa gtgtttccca cttgtatttt cccatcgtat
    16741 gataaggaag aatctctact ctgtctacat ttgacaaggt ttgaataaag ttgtctagtc
    16801 ggatcaaata ttcatcataa tcacttcgct caggaactag tacatggcga atccaaaccg
    16861 gtttgttgat ttttgacaaa tattgagcca tttctaaaat attttcattg tcccaatgcg
    16921 ttagtttttt atgctcttca ttatcgatgt gtttgatgtc aaataacagt aaatctgtat
    16981 acttcatcaa ttcttgaaaa cgtgaaaaga aaggttcttc atacgtaaaa ggttttccgc
    17041 atgtatccaa tgtagtatgc atcttccgct cttttgcttt tttaaatagt tcgatcaaaa
    17101 aatcgatctg caatagaggt tctccaccac taacagtaat accaccttta tctccccagt
    17161 aaggtctgaa gcgttctgct ttatctaaaa gttcatctgc tgtgtattct ttccctccgc
    17221 caatattcca agtatctgga ttgtgacaaa attggcaacg catgcggcat ccttgcataa
    17281 acacgacaaa gcgcagtcca ggtccgtcta ctgaaccgaa agtttcgata gaatgaacat
    17341 agccgattgt tttttcttcc atttaagttc acatcttttc tttgatttca ttttaagtac
    17401 aagttagctg tcgaagcgaa gctttctgaa caaaattttt cgcaattgcc gcttcgttat
    17461 gtgtaaaatt tacagggacg gaacatacaa gtcctgcccc tgtaaatcgt ttctttcaaa
    17521 taagcatact gattattcga ttttgatgcg atggctattc aacgtccttt cgacgctaag
    17581 tcgaaaaact tttttcacga actttttaca ttctgtcgtg tgaagttctc atcaagacat
    17641 ctaattgttg ttctcttgtc aagtcacgga atttaactgc ataaccagat acccggatcg
    17701 tcaagttcgg atatttcatt ggattttcca ttgcatcacg caataaatca ttactgaata
    17761 cattgacatt caaatgataa gctccttttg agaagtatcc atctaagaca ttacgcaagt
    17821 tttcgattct tgtatcttgt tcttttccaa gacctgaagg attgattgtt tgggtatttg
    17881 aaattccatc caatgcacag ccgtaattca ttttagctgt tgagttcagg ctagctaata
    17941 acccactttg ttccgcattg taacttggat ttgctcctgg cgcaagtggt tttccagctt
    18001 cacggccatc aggagtattt cctgtatttt taccgtaaac aacatttgaa gtaatcgtca
    18061 ataatgaagt tgttggtttt gagttgcggt aagttttatg acgtttgatt tgtcccatga
    18121 agtattccaa gatccatgaa actaattcat ctgcacggtc atcatcattt ccgtatttag
    18181 ggaagtcgcc atccacttca aaatcaactg ctattccttt ttcattacgg attgctttta
    18241 cttttgcata tttgatcgct gacaagctgt ctgccgcatg agagatccca gcaatacctg
    18301 tagcaaatgt gcgttgcaag tcagaatcca tcaaagccag ctgagctgct tcataagaat
    18361 atttatcatg catataatga atgatgttca acgtattgac atatagttct gctaaccaat
    18421 ccatcatatt catgaagtta tgtttcactt cttcaaagtc tagtatttct gatgtgattg
    18481 gacgatattc tggtccaact tgggcgccag tcacttcatc gactccgcca ttgatggcat
    18541 ataatagtgt tttagccaag ttagcacgag cgccaaagaa ttgcatgtct tttccgacaa
    18601 ctgttcctga tacacagcaa gcaatcgcac aatcatcaga accccattgt tcacgaagct
    18661 gattatcatt ttcgaattga atcgaagaac tttcgatagc gattctgcta gcatatgttc
    18721 tgaatccttc tggaaggtgt tcagaataca aaacagtcaa gtttggttct ggtgaaggtc
    18781 ccatatttgt taatgtatga agcagacgga aatcattctt tgtaactaat gaacgaccat
    18841 ctaatcccat acctgcaatg gataaagtag cccagatagg gaatcctgag aataattggt
    18901 tatattcagg tgtacgcgcg aatttgacca tacgtaattt cattactaag tggtcgatca
    18961 attcttgtgc ttctttttct gtgattactc cattatccaa atcacgttgg atatagatat
    19021 caaggaatgc tgagatacgt ccgattgaca tcgcagcacc attttgtgat ttgattgctg
    19081 ctaaatatcc gaagtatagc cattggacag cttcttttgc atttgcagct ggtttagaaa
    19141 tatcatagcc atatttcgca gccatttctt tcatttgcgc taatgcacga tattgttcag
    19201 tgatttcttc acgtaaacga atcacttctt cactcattgt atgataacca gtcatatcat
    19261 gatctttttt cttttgttcc atcaaatagt cgattccata caatgccaca cgacgatagt
    19321 caccgatgat acgtccgcga ccgtaagcgt ctggaagccc tgtaatgatt ttgtttttac
    19381 gagcagcacg catttcagca gtataagcgt caaatacacc ttgattatgt gttttgcgcc
    19441 agtcagtgaa aatatgtgtc aattcatcgt ttggtgtata gccattgctt tttagcgcat
    19501 tgttagccat attgattccg ccgaacggca tgaatgcttg tttcaaaggt ttgtctgtct
    19561 gtagaccgac gattttttcc aaatctttat ccaaatagcc tgcatcgtgt gaagtaatag
    19621 ttgatacgat ttctgtgtcc atatcataga caccgttacg ctcgaattgg atatcattta
    19681 acttttgcaa ttcatcccat aatttttctg ttgcttctgt tgggccttcc aaaaagctgt
    19741 catcaccgcg atattcagtg tagttatttt gaataaagtc tctagtgtct acgccttctt
    19801 tccatttggt acctttaaat ccattccatt gctccattgt atggacctcc taaatttctg
    19861 ttttatgtaa cagtttttac gtgattagta taacacagca ccctatcttt gcaagggttt
    19921 tcttgttttt tatttaacaa tttttatgtt ttatttacat atatttaaat caacgttgac
    19981 ataccaacat ttaagcattg tgaacacttt catctttttt tgcaaacgta taaggattta
    20041 ttaaaaatga atgaaaaaga gacgccacct gtattaatac agatggcgtc tcttcattaa
    20101 aatactacaa cagttacgac tcgatgaaaa tctcaggagc atcgataact tcgatgactt
    20161 ctccaccttt tttctcatcc atcgcaaatg atccattcga tgttcgatca cctataggga
    20221 aatcagttgg gtcaatcgtt aactgctgcc ctttttgatt catcaaggtt atttcttttg
    20281 atcttccttc cgacatatag ataatacggt gcggattttt tttcagctca cgcatgaccg
    20341 tcactccccg cttcgcacgt gaagtttggc tgatttcttg agcaagcatg cgtttaacag
    20401 ctcctcgctg agtaacgatt acgatcggcg tatcaccttc tgctaacaca agcagcccgt
    20461 tgataatcct atctccatct ttcaaattca tagacttcac tccagctgcc tttgctccaa
    20521 caacgggaac atctgttaaa ggatagcgca aacccattcc ttgatgcgtt actaagaaaa
    20581 catctaagcc tggctgatct tctttcagat acacagcaat cagttcatct tcttccgcct
    20641 ttagcttcat acagctgagc ggacggctct tataggtgcg ccatggttcg aactctgtca
    20701 ttcgtgtttg cttgatcaat ccattcttgc tgatgaatac aaatgttttc tctgcttgaa
    20761 ttttttgata tgggaaaaca gctaaaatcg attcgtctac agacagattg gagattgttt
    20821 gagaaatgtg ctcaccagca tctttccatt tcaaatcggg tagttcatgg acagggcggt
    20881 agatcacatt tgctttattt gtgatcagaa gaacatggtc caatgtactc aattcacctg
    20941 agtatagcaa gtagtcgcct tctcgcatcc cgatttcttc tggtttggaa gcactataag
    21001 aacgaaggct tgttcgcttc acatagcctt ctcgcgtcac actgacaatt acgtcttctt
    21061 gtgccactaa gacttgcgta tcaattttga tttcttcgat ttcgtcttcg attgtcgtta
    21121 gacgagcgga agaataattt tttttcactt ctcgcaattc ttttttcata acagagaaaa
    21181 gctctttgtc atttgaaagg attttattca actctgtgat ttgcgcaatc aagctttcgg
    21241 attccttttg aagctctgtg atatccgtgt tcgtcaaacg gtataattgc aatgtgacaa
    21301 ttgcttctgc ttgttcttct gtaaattgaa agacgtcgac taaattcttt ttggcgtctt
    21361 ttttgtcttt actttcacga atcgttgcga ttacttcatc cagaatcgat aaagctttca
    21421 tcaatccttc tacgatatgc tgtctttttt gagctttggc taaatcgaat tgactacgtt
    21481 ttgttatgac ttgctttcga tgctcgatat aacttcttaa aatgtctttc agtccaactt
    21541 gatgcggagt catatggtcg atagcgacca tattgaaatt gtaattgatc tgtagctcag
    21601 tgtttttgaa caaataattc aaaattcctt gagcattggc gtcttttttc aattcgacga
    21661 caatttgcag acctgttcta tcactttcat ctcgtacttc agctattcca tcgattttct
    21721 tattcaaacg gatctcatcc atttttttca ccaaggtcgc tttatttact tcatatggga
    21781 tttctgtgat cacgatttgc tgtttattac ccttcattgc ctctatcttt gttttcgaac
    21841 gaagaataac ttttcctcgt cctgtttcat aagctttttt gatttcatct tttccttgca
    21901 agatccctcc tgttggaaag tcaggtccag gaatatattc catcaatttt tcaaggctcg
    21961 cttgtggatg atcgatcata tagattgtgc catcgatcac ttcagctaag ttatgagtcg
    22021 ggatttctgt tgcatatcca gcagatatac ctgtcgatcc attgacgaga aggtttgggt
    22081 atcgagctgg aaggactgtt ggttcttttt ctgtatcatc aaaattccac acgagatcga
    22141 ctgtttcttt ctcgatatct gctagcatct ctccacttaa cttggataat ctggcttcag
    22201 tataacgcat cgcagcaggt ggatcaccgt ccatactccc gttattcccg tgcatttcga
    22261 tcagtacttc ccgcaacttc cagtcttggc tcatgcggac catcgcttca taaatactga
    22321 tgtcaccatg aggatgataa ttccccatga tatttccgac agattttgct gatttacgga
    22381 atcctttgtc atacgtattg ccatctttgt tcatggcaaa taatatccgg cgttgcactg
    22441 gcttcaatcc atcacggata tcaggcagtg cacgttcttg gataatatat ttggaataac
    22501 gtccgaagcg gtcacccatc acttcttcta aagttaattc ttgtacttct tgtcgatttt
    22561 ccatcaggtg acctcctatt ctacttcaaa taactgattg ttttcatttt tatctgctcg
    22621 ttcttcatct aataaatcat tagaaactga aggagagatt tcagtatcct cttttttatc
    22681 taagatactg ccatcttctt cgagcgtaaa ttgcacgtga ttttcgatcc attttcttcg
    22741 aggttctact ttgtcaccca tcagtgtggt tactcgacgt tcagcttgcg cagcatcatc
    22801 gatacggacg cggatcaacg tacgggaagt cggatccatc gttgtttccc acaattgttc
    22861 cgcattcatc tctccaagtc ctttatatcg ctgcagcata tatcctttgc cgactttttt
    22921 aatcattgct gccaattcgt catctgtcca cgcatattca atgacttgct tctttccttg
    22981 tcctttggat actttatata aaggcggtaa cgcgatatat actttcccag cttcaatcag
    23041 cggtttcata taacgataaa agaaagtaag cagtaatact tgaatatggg cgccgtctgt
    23101 atccgcatcg gtcatgatga ttactttgtc gtaattacag tcttcgatag aaaactcagg
    23161 accgactcct gcaccaatcg tatagatcat cgtattgatt tcttcatttt tcaagatatc
    23221 ttgcatttta gctttttcgg tattgatgac ttttcctcgt aaaggtagaa ttgcttggaa
    23281 ctttctgtct cttccttgtt tagcagatcc gccagcagaa tccccttcga ctaaatacaa
    23341 ttcatttttc ttagggtttc gggattgtgc gggtgtcaat tttcctgaaa gaagagattc
    23401 gcctttttta cgcttcttcc catttcggct ttcttctctg gctttacgtg ctgcttctct
    23461 cgcttctctg gctttgattg ctttacggac caacatctgg ctcatttcgc tattttcctg
    23521 caaataaaag cccatttgtt cactgatgac gttatctact acggtacgag caactggcgt
    23581 accaagtttt tcttttgttt gcccttcaaa ttgcagaaga ttttcaggta cacggataga
    23641 aagtacagca gctaaccctt ctctgaaatc actaccttcc agattcttgt cccgttcttt
    23701 cagcaaacca acttttctcg catattcatt ataagatttc gtcatcgccg ctttcatacc
    23761 agcttcatgg gttcccccat cttttgtccg aacgttattg acaaaagata aaacattttc
    23821 tgaataaccg tcattgtatt gataagcgac ctctacttcg ataccttctt tttctcctga
    23881 aaaataaaca actggtgtca atgtatcttt ttcttcattc agataatcaa caaattcttt
    23941 gattccttct tcgtaatgaa aaatttcctt gactggttct tctccacgaa gatcagaaag
    24001 agagatttta acgcctttta gaagaaaggc agattctctt aaccgttcgg ctaaaatttc
    24061 atatgaaaat ttcgttgtac tgaatattgt atcatctggt aaaaaatgga cagtcgtccc
    24121 gtttgctttt tttgtttttc ctatcttttt caaagtaccg tcaggtttac cgccgttttt
    24181 aaatttctgc tgatactcta cgccatctct aacgatcgtt acaattagcc atttcgataa
    24241 ggcatttacc acactggctc ctacaccgtg cagtccacca gaagttttgt accctccttg
    24301 accaaacttt ccgccagcat gcagcacagt aaaaatcact tcaactgttg gtattccgga
    24361 agcatgcatt cctacaggca tcccacgtcc gctatctgca acagttatac tattgtcttc
    24421 atggatggtc acatcaatct cactgccata acctgataat gcttcgtcaa ctgcattatc
    24481 gacgatttca tagactaaat gatgcaagcc tcggctgtca gtggatccaa tgtacatccc
    24541 tggtcttttt ctaactgcct ctaatccttc caatacttgg atggacgagt cattgtattc
    24601 gttgtttact ttttttgcca aggcaaacgc tccttcaata atactttgcg gcaaaaccgc
    24661 accacgatta tcatactaga aaaggtcgtg aaaaaaaaga gggtagacga gaaatcaatt
    24721 ttaattatct cctaaccgct ttttcgacat tcccctctat cgatcatctc ttctttgtgg
    24781 tcatttttat ttttggtttt ctttcaactt accaagttca atcttaatac aacgatccat
    24841 tacgatatct gtacgtcctg cttttcttag catctcagct gcttctttgt tttcaatccc
    24901 taattgtgcc cagaacactt tagcatcagt tttcaaaaaa tcacctgcta tttcaggtaa
    24961 aaattcactc cgtctaaata catctacgat atcgattgga tcatcgattt ctgtcaaatt
    25021 cgcatagact ttctcgccta gaatttcttc accagccaat aatggattta ctggtatgat
    25081 tttatatcca ttgtcttgca atacctgtgc aaccatgtaa cttgttcgtt ctggtttatt
    25141 gctcaagcct acgacggcaa tattttttgc ttcttccaaa tagttgaaaa tcgtttcttg
    25201 tgttggattt tttacagaca tttctgccac cccttgcttt tctttttatt ccatctatta
    25261 tacttgtatc tttcattttt tcaattgttc ctgttaccta ttcatgctaa aataaaggat
    25321 agttttcaac ttgaaagagg gtaaaaaatg aaattgatct tattacttat tttagcatat
    25381 cttttaggtt ctattccttc cggtgtctgg attggcaaat tgtttttcaa aaaagatatc
    25441 cgacaacacg ggagcggtaa tactggaacg accaatacat ttcgtgtatt aggcaaaaca
    25501 gccgggattg ttgtcctgct tatggatatt ttaaaaggta cattggctac ttgtttgccg
    25561 atgatcttcc acctttctgg gattaaccct ctttggtttg gggtatgcgc cattcttggc
    25621 catacatttc cgatttttgc tgggttcaaa ggaggaaaag ctgttgcgac aagtgcagga
    25681 atgcttttag gatttaatcc tgtatttttt gtttattctt gtatcatctt tatcgttagt
    25741 ttattttgta caagtatggt gagtttgagc agtatgatca gtgcagtatt gatcacttta
    25801 tctacgatcg tccttcctta tgtagctcct gtgattttgg ctaagcccaa ttggttactg
    25861 acgatcattg cctttttagt ttcttccttt atcttttatc ggcataaaga caatatcaag
    25921 cgtatcagaa accatactga aagtcgaatc ccgtttggtt tgaatagcag caagaaaaaa
    25981 tcctgatact ttttcattcc aaagagataa aacgggagtg tgacaaatcg atgtcacact
    26041 cccttattac ttacaggata gcttttttac acaccttggt aaacggtgtt ccctgctaac
    26101 ttctcggtca caacctctta cttcactgtg atggaatatt ttgtcttgaa gatttcatgg
    26161 gcacctagtt gattgatccc tactttttcc atcaagtttc cactactgtt caccgtgtca
    26221 gcgattccgc accacggttc gatacaaacg aaaggcgctt cttttggata aggagaccag
    26281 atccctacat aaggcatatc tttatagctt aaagttacgc tatgggaaga tttttcactt
    26341 cgaatggcgt acgcatttag accccttgtt tcaaaaacca aggcatcttg ctcaaaaagt
    26401 tcacgattca aagccagatt cgtatttgtc tgacctaacg tcttttgatc catatcgatg
    26461 aatgggcctt tcaatggtag gcgtaatctt gatttcaatg gagaaaatgc cagatagaaa
    26521 tcttcaaatg acaatccttc ttcaagaggt acattgaaag caggatgtcc accgatagaa
    26581 aagaacattt ctttttcacc ggtatttgcc acttgataat gaaccgtgat gccgtctcct
    26641 cctaattcat agctgatcac taattcaaaa tcaaatggat acttttctaa tgtctccggc
    26701 gtactcctta agatgaaact agccatttct tgtccatgtt caatgacttc aaaatccata
    26761 tcccgagcaa atccgtgttg ccccatttga tatgttttcc cttgataaac atactgatca
    26821 tcttttaaac gtccaacaaa agggaataaa accggtgcat gtctgcccca atacgctgga
    26881 tcaccttgcc agatatactc taaaccatgt tcttttgatt tcagactgat caattccgca
    26941 ccgtgttcag cgatttcggc gatcagctgg tcattttgaa taataacagt cattgccatt
    27001 cctccaactt cgacttactt tttaaaatgg gaaggctgga acaaacattt ctatctgccc
    27061 cagtcccctt acttctaata actgtgtttt gctaactcgt ctttaaaacg ggaattcaat
    27121 acttttaaat aagttccctt catgcctaac gaacgagatt caatgattcc agctgattcc
    27181 aattttcgta aagcattgac gatcacagaa cgagtgatgc cgatactatc ggcaatatta
    27241 gaagcagtca gacgaccttc atctccatcc aatgcttcaa agatagcttg tacagccttc
    27301 aattcgctat aagataatgt gttgattgcc atttggacag ccgttgcact ccgtacatct
    27361 gcttcgatgc ttcgagactt ctgataaagg atctgcatac cgacaaccgt agctccatat
    27421 tctgctaaga ctagatcgtc atctgcaaac gattgttcca tacgagataa gataatcgta
    27481 cccaaacgtt cgccagctcc aaagatcgga acgacagtcg tcagaccaaa tggatatttc
    27541 tctcttgact ctaccggaaa tgcagtaata tcactagtaa ttgggatatt agcttcagtt
    27601 ttcatcaact gtgccactgt atcagtataa gattcaggaa attgtttctc aacaaacatt
    27661 gttttgattc gttcattgtt cacatcgtga cgttcattat aacctagtaa tactccgtca
    27721 cttcctatga tataggtgtt gctgtcaaga ataccaccta gtgtgacagc cattttatcg
    27781 taaggcaaat ctgcttgcat gtcaaaagta ttcttttgct gcaaaagtgt gtttatcttg
    27841 cgggttttcg ttagcaagtc tgtcattgtc gtcctccgat tttttataaa atatagcgac
    27901 ttaaatcttc atttgccgca attgagccaa gtttgtcatc cacatatgct tctgtgatcg
    27961 ttatttctcc catttgcata tctggtgctt caaataatag atcttctagt agtttttcca
    28021 aaatggtatg cagtctgcgt gcgccgatat tatctgtgtc acggttgaca tcgaaagcga
    28081 tttgagcgat ccgttcaatt gcttcttttg taaacgtaac tgacacattt tctgtaccga
    28141 tcaacgcgac gtattgtttt actaaagcat tatttggttc ggtcagaatc ttcacaaaat
    28201 cttcagctgt caaatcatct aattctaccc gtatcgggaa gcgtccttga agttctggaa
    28261 ttagatcact tggtttagac aaatggaaag ctcctgaagc aatgaatagg acgtgatctg
    28321 tttggatgat cccgtatttt gtgtttactt gtgaaccttc gacaattggc aatatgtctc
    28381 tttggacgcc ttctctggat acttctccag attgctgtga tttagaagtg atcttatcga
    28441 tttcatcgat aaaaataatt ccactttctt ccgctaattt gattgcctca ctatggatat
    28501 cagcatcctt gactaatttt gctgattctt ccttgatcaa taactctaaa gcttctttga
    28561 ctgttaccgt ccgttccact tttttcttcg gtgtcaaagc acctagagtt tcatttaggt
    28621 cgatccccat ttgctccaat ccattgttca tcattggtgc aggtttcttt tggtcttcaa
    28681 cttcaatggt tacttcacga ctatccagta aacccttttg tagttgttcc aagactgtac
    28741 ggcgattgat tcgaatatct tctgtcactt cttcttgcgg ttcagcggta ttttgctgac
    28801 tagcattaaa aagattcatc atttgttcaa attgattagt ggattgtttc tgttcttttt
    28861 tgataccagg taccaagatt tttaccaaac gtttttctgc atttttttta gcacttgtgt
    28921 atacacggct gtactgctct ttttctacga tttggatcgc attttctact aaatcccgaa
    28981 ccattgattc tacatctctt ccgacataac cgacttcagt aaattttgtt gcttcgactt
    29041 tgacaaatgg tgcattgacg atttttgcca aacgtcgtgc gatttctgtt ttaccgaccc
    29101 ctgtaggtcc gatcatcagc atattttttg gtgtaatatc ttgttgcatt ttttcatcta
    29161 actgcaaacg gcgatagcgg ttacgcaaag caacggcaac agattttttt gctgcgtgct
    29221 gtcctacgat atattcatct aattcactta cgatttgtct tggtgtttgt tttaattcat
    29281 acatatctac tcacctctta cagttcttct acgataatat tatgattagt aaagatacag
    29341 atatctgcgg caatatttaa cgcattttct gcaatttctt tcgcactcat gtcgttttga
    29401 gcatagtttt tcattgctcg ggctgcagcc aacgcaaaat tccctcctga tccgatagct
    29461 aagataccat catctggtgt gataacttct cctgtacctg aaacaagaag catttcttta
    29521 tcattcatta cgatcagcat cgcttcaagt ttttgcatag attgctgtgt ccgccattct
    29581 tgagctaatt cgacagctgc gcgttgtaga ttcccgttat attcgtttag tttgccttca
    29641 aatttttctt ctaaagtaaa cgcatctgca acacttccgg cgaaacctac aacaacttct
    29701 ccattgtaga tacgtcgtac tttctttgca gtgcctttca tgacaacaga ttcacccatt
    29761 gttacttggc catctccagc catagcaaat ttcccgtctt tttcgacggc acagatagtg
    29821 gttgaatgaa atggtgattc gaccatgttt gtttcctcct aaatttgttc ttttccttat
    29881 ttttacgctc ttggatggaa agaacgatag tttttttgta aactttcttt tgttacatgg
    29941 gcatagatct gggtagtcga taaattggca tgtcctagca attcttggac tgtccgcaaa
    30001 tctgccccat tatttaataa atgagtagca aatgtatggc gcagcatgtg aggatggata
    30061 tcactatcca aactgctttt tttgatcatt tgattcaaga catattcaat tccagtaggc
    30121 gtgattggtt ttccatgatg attcacaaaa acgatatcat gctcttcgtg atattttgtc
    30181 atcaatacct ggcggccttc cctgaaatat tcttgcagag catcttgagc aaaagaacct
    30241 agaggagcat atctctcttt gttcccttta ccatggatca acatgacctc tgaatcaaaa
    30301 tcaatgtctg ataccgacaa actgctacac tcacttaaac ggatgcctga cccgtaaagg
    30361 acttccagta aagcgcggtt cctcaaatcc aaaggactat ctcctgctac gctgtcaaaa
    30421 agtgcttgca tttcattttc ataaaaaaaa cgtggcagtc tcaaattttt ctttttgaga
    30481 tgaacgtaag aaaatggatt ttcttttatc acttcttctt tgagaagata ctgataaaac
    30541 gaacgtaggc tggctatctt gcgactgata gaatttctgc tatagctagg acgatgatca
    30601 gctaatgcac tcaaatagac acgaaccgta aaatattccg ccttcagcag atcagcgtct
    30661 ccagattcct ccaaaaaatg tttgaaatgg agaatgtctt cttcatatgc ttctttcgtt
    30721 ttttctgaat atcctctttc gacaatcaaa taacgcaaaa atttttgttc ccacgtggct
    30781 tctgtcattt tctcaccttc cttttgtccc agagaataat ttagcacagc gtttataaaa
    30841 agacaaataa attgtcagaa tttaaccaat atttctaagt ttgttcacaa ttggcgcaat
    30901 tcctcacatt ttgtttgaga aattcttaag aaaaac
//
LOCUS       DAZKOR010000026        30824 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000026 DAZKOR010000000
VERSION     DAZKOR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30824
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.026"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(215..2290)
                     /gene="glyS"
                     /locus_tag="ACG8ZM_001103"
     CDS             complement(215..2290)
                     /gene="glyS"
                     /locus_tag="ACG8ZM_001103"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0492076.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2292..3209)
                     /gene="glyQ"
                     /locus_tag="ACG8ZM_001104"
     CDS             complement(2292..3209)
                     /gene="glyQ"
                     /locus_tag="ACG8ZM_001104"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0492077.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3593..4405)
                     /gene="recO"
                     /locus_tag="ACG8ZM_001105"
     CDS             complement(3593..4405)
                     /gene="recO"
                     /locus_tag="ACG8ZM_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0492078.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4548..5447)
                     /gene="era"
                     /locus_tag="ACG8ZM_001106"
     CDS             complement(4548..5447)
                     /gene="era"
                     /locus_tag="ACG8ZM_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0492079.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5462..5857)
                     /locus_tag="ACG8ZM_001107"
     CDS             complement(5462..5857)
                     /locus_tag="ACG8ZM_001107"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0492080.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5841..6317)
                     /gene="ybeY"
                     /locus_tag="ACG8ZM_001108"
     CDS             complement(5841..6317)
                     /gene="ybeY"
                     /locus_tag="ACG8ZM_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0492081.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6334..8526)
                     /locus_tag="ACG8ZM_001109"
     CDS             complement(6334..8526)
                     /locus_tag="ACG8ZM_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0492082.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8550..9521)
                     /locus_tag="ACG8ZM_001110"
     CDS             complement(8550..9521)
                     /locus_tag="ACG8ZM_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0492083.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10227..11606
                     /gene="brnQ"
                     /locus_tag="ACG8ZM_001111"
     CDS             10227..11606
                     /gene="brnQ"
                     /locus_tag="ACG8ZM_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0492084.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11762..12208)
                     /locus_tag="ACG8ZM_001112"
     CDS             complement(11762..12208)
                     /locus_tag="ACG8ZM_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0492085.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12235..12411)
                     /gene="rpsU"
                     /locus_tag="ACG8ZM_001113"
     CDS             complement(12235..12411)
                     /gene="rpsU"
                     /locus_tag="ACG8ZM_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0492086.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12573..13001)
                     /locus_tag="ACG8ZM_001114"
     CDS             complement(12573..13001)
                     /locus_tag="ACG8ZM_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0492087.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13093..13953
                     /locus_tag="ACG8ZM_001115"
     CDS             13093..13953
                     /locus_tag="ACG8ZM_001115"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0492088.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14037..14387)
                     /locus_tag="ACG8ZM_001116"
     CDS             complement(14037..14387)
                     /locus_tag="ACG8ZM_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0492089.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14403..16130)
                     /locus_tag="ACG8ZM_001117"
     CDS             complement(14403..16130)
                     /locus_tag="ACG8ZM_001117"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0492090.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16343..17269)
                     /gene="trxB"
                     /locus_tag="ACG8ZM_001118"
     CDS             complement(16343..17269)
                     /gene="trxB"
                     /locus_tag="ACG8ZM_001118"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0492091.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17414..18847)
                     /locus_tag="ACG8ZM_001119"
     CDS             complement(17414..18847)
                     /locus_tag="ACG8ZM_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0492092.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18847..20250)
                     /locus_tag="ACG8ZM_001120"
     CDS             complement(18847..20250)
                     /locus_tag="ACG8ZM_001120"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0492093.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20480..21382
                     /locus_tag="ACG8ZM_001121"
     CDS             20480..21382
                     /locus_tag="ACG8ZM_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0492094.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21423..23309)
                     /locus_tag="ACG8ZM_001122"
     CDS             complement(21423..23309)
                     /locus_tag="ACG8ZM_001122"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0492095.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23406..24257)
                     /gene="licT"
                     /locus_tag="ACG8ZM_001123"
     CDS             complement(23406..24257)
                     /gene="licT"
                     /locus_tag="ACG8ZM_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFQ0492096.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(24715..26499)
                     /locus_tag="ACG8ZM_001124"
     CDS             complement(24715..26499)
                     /locus_tag="ACG8ZM_001124"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492097.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26499..28250)
                     /locus_tag="ACG8ZM_001125"
     CDS             complement(26499..28250)
                     /locus_tag="ACG8ZM_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0492098.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(28392..28616)
                     /locus_tag="ACG8ZM_001126"
     CDS             complement(28392..28616)
                     /locus_tag="ACG8ZM_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0492099.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28867..30453
                     /locus_tag="ACG8ZM_001127"
     CDS             28867..30453
                     /locus_tag="ACG8ZM_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0492100.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8603 a         6629 c         5501 g        10091 t
ORIGIN      
        1 agcgacagtt taccaataaa tttcaactga attatctaaa agtagatcag ttagtttaga
       61 taattcagtt gaaattgacc gactcaaatt acttgtataa tacctaggaa aaaattaact
      121 agcaccacgg gtatagttat ttatatttat aacaaataaa ccagagaaat agcctatttt
      181 gtgcctattc tctggtttat ttacatatta gaaattattt tgtgatgatt tgatctaaac
      241 ttgctagcga caaagccatg gcagcaattt gacttaattg cgttaatcgg ttcttacgta
      301 ctgctggttc ttctgccatg accatcgtct gatcaaaata attttcgatc agtgggcgaa
      361 ggcttacaag ctgatcataa ttttcgttga tcgtttggtc agcaaagctt tttttcattg
      421 cctctactgc tttgtgcagt tctttctccg cttcattttc gaacagttca gggtctactt
      481 ctgattgttt ttcaagctcc gcttttttcg ctaagttcac gacacgtgtc aaagcttcca
      541 tcgatggttt gaagtcttca tctgcaagat gctgcttgaa caattgtgct gctgcaaata
      601 cttttgtcag atctttttgt tctgcattca aaacagcttc gatgacatca tgacgaatat
      661 tcttcgttgt caataattgg cgcagacggc ctttgataaa gtcgatcacc tgttgttgcc
      721 cttctgataa accaatgccg aaacgatccg tatctttact gataacatca ctaatcgttt
      781 cttgcagaac agacaaagga aaggcccatt ttttcgattc gatgatccga acgacaccgt
      841 atgcttggcg acgtaaggca taaggatcgt ttgacccgct tggaatcatc ccaactgcaa
      901 agaaagaaaa aatgctatct aatttatctg caatcgctaa aacagaacct actgtagact
      961 gaggtagctc tccttcactg gatataggca tgtaatgctc acgaatcgct gttgctacag
     1021 caggtttctc gcctttcagc agagcatatt tttcgcccat gatcccttgt agttcaggaa
     1081 attcccctac catgttggtt actagatcaa atttatagat ttcagcagct cgttgcagat
     1141 ccatcaattc ttcctcagat agtcctactt gttcgccaat gatacgggca atgacttgta
     1201 cccgctgcat tttctcatag atactaccga ttttttcatg gaaagtaaca gtttttagtt
     1261 tttcaacgta ttggtcgatt gaagctttct tgtcttcatt ataaaagaat tccgcatctt
     1321 ctaaacgagc cgtcaatact ttttgattgc ctcgaaccac attttccaat tttacccgat
     1381 caccattacg agcagaaata aaatgaggaa gcagcatacc ttgatcgttc cgtacctcga
     1441 aataacgttg atgttctttc atagaagtga ctagtacttc ttcaggaaca gaaagatatt
     1501 tttcttcgaa gtcgccagaa aagacagttg gatattcgac taaattgttg acttcttcta
     1561 gtaaatcttc gtctagatca ataacccagt gatttttttc ggctaattca tttgcttgtg
     1621 taacgatcat ttcttgtctt ttttgcggtg aagcaataac gaattgttca ttcaattttt
     1681 cttcatattc ttttgcatgc gcaaatgtca catcttcgcc taagaaacga tgtcctcgag
     1741 aggctctgcc tgttttgaca tctaagatct caaacgggat tacttcgtca tctaacaatg
     1801 ccacgatcca gtgaataggg cggatatatt caaaatcgta ttctgcccaa tgcatcgtta
     1861 ctgggaaagt gaggctttta atgacatctg ctaaacctgt caatacttct ttcgcttctt
     1921 tgccgtgagt gtatttcgtt acatagacat attccacgcc ttttaattct ctaaaagtga
     1981 tagcatctgt cgtcagacct tgtccgcgga caaatccttc agctgctttc gtccagtttc
     2041 catctgcgtc caaagcaatt tttttggctg gtcctttgac ttcttcttca ctgtctgctt
     2101 gacgttctgg aatgtttgtt actttgattg ctaagcggcg cggtgtagaa aacgtttcga
     2161 tcgtatcata agtcaattgg ttttcatcta aaaattttgc tgttttttct tctaattgtt
     2221 tcatgcttgg tgtgacaaca tgagcaggca tttcttccag cccgatttca agtaataagt
     2281 cttttgccat cttattcagc ctccttcatg ttttctggat ttgctgtgtt tttattcaat
     2341 aaagggaatc ctaatttttc gcgttctgcc acaaagattt tggctacagc acgagccatg
     2401 ttgcggatac gggaaagata acctgctcgt tctgttacgg atactgcacc gcgagcatct
     2461 aataaattga atgtatggct gcatttcaaa atataatcat acgctggatg aacaagattt
     2521 tcatcgatac agcgtttagc ttctttttca aaacggtcaa agttgcttag tagcaagtct
     2581 tggtcactta cttcaaaaga atatttggaa tgttcatatt ctggttgttt gaagatctcg
     2641 ccatatttca caccatcagc ccattccaga tcatagacac tttcaacttc ttgaatatat
     2701 gaagcaagac gttctagtcc ataagtgatc tcagaagtaa ctggtttaca aggtagtcct
     2761 ccgacttgct ggaagtacgt gaattgtgtg atctccatcc catcaagcca tacttcccaa
     2821 cccacaccgg cacaccccat cgatgggttc tcccagttgt cttcaacaaa acgaatatcg
     2881 tgttctaatg gatcaattcc taaaagacgc aagctttcta aataaagctc ttggatattt
     2941 tcaggtgaag gtttcattac gacttgaaat tgatgatgct gatacaaacg gtttgggttt
     3001 tctccataac gcccgtcagc tggtcggcgg gatggctcta cataagcagc attccatggt
     3061 tccggtccga ttgcccgtaa gaatgtatat gggctcatcg tacctgctcc tttttctgtg
     3121 tcataagctt gcatcagcat gcagccgttt gatgaccaaa atttttgtaa tgttaaaatc
     3181 atatcttgga cggtcaattt tttactcatc tgttttgccc ctttcaattt ttaagcgttg
     3241 ctgtcaataa gaatacagga aagaaaacga caaaaagtcc ctatgtctga ttacagacat
     3301 agggacgaat aatttcgcgg ttccacccta cttccgaata aagattcggc agcttcattt
     3361 atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc ggcttccact ctcccgaact
     3421 cgctaaacca cgtgtttttt actgatcttg gtcaatgtac gtattcattt tgcagttttc
     3481 ttgtttatat aatagatcct ctcagtgaac gatctattta atctacggtt aataatagac
     3541 ggaagtccct gtatttgtca aatagttttg atttttttgt tgtatctttc atttattctt
     3601 ctttttcagg tatctctttt tcaggcatat ttttctctgg cgtctttaat acgtgtgccc
     3661 aagacttcat ttgatcaatg aactttttgc tttttagatg aataccgaca tattcatcat
     3721 acaactggtc aatgaggtca cggattgcct gcttcgtttc ttctttcaag tggattccac
     3781 tgatcttatc ataagaaata gccgaaaaca ttcgaacgaa ataaacagcg cgaggatctg
     3841 catgataacg gtgttcgtcc attggccagt gtttctcgca tatcacaccg ccagatttgg
     3901 aagaatagtc aaattttcct tttgtttccc cgcatacgac acagtgcgtc caaattggcg
     3961 cgattccgaa gcgttgcagt atttgaactt caaagatatt cgtgatgatt tctgcatcat
     4021 ctccttgatc catccgctgc agagcttgtt gcgtaaattg gaataaatgc gggtcatatt
     4081 gatgatcttc gattgctgca tctactagat ttagcaaata ggtaccataa ccgctcaaaa
     4141 agatatcttc ttggatgttc cgaaacgcat ggacgtcttt gacactgctt aaaaacgaca
     4201 aaccttcact tcgaaaatta ccgatataga ctgcttcggt aaaggggagc aaagccggag
     4261 ctaatggctg attttgccga tggattcctt tgatataaaa catcattttg ccagaagatt
     4321 ccgtaaagat tttgactaat ttatcttttt ctttgtaatt acggctagat aaaatcaagc
     4381 ctttcgattc tgcaagctgg ctcatattct ccctcctaaa aaataaatct ggaaaagcag
     4441 attctgccga tccagattat ttatgtttcg atcttccgcc gtttttctca acgtgcggat
     4501 ttttatccag tttatttcta tacttaacct ataagaagtt cagaatatca gtattcgtct
     4561 tttcggtagc caaagtcttg cagatagact tttttgtctc gccagtcttt ttggactttg
     4621 acccaaagtt ctagaaatac tttgtctcct aaaagatttt cgatatcctt tcttgctttc
     4681 gttcctactt gcttcagcat ttttccgcct ttgccgataa tgattccttt ttggctatct
     4741 ctttcgacga tgatcgtggc ctggatatgg attttatcat tctcgtttcg cttcatcgaa
     4801 tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg ttaaaaacaa gactttctca
     4861 cggatcaatt cagaaacgat aaagtattca ggatgatcag tcacttgatc atctggaaaa
     4921 tattgtgggc cctctggcat ttcactgact aatacatcca ttaagcgttc gacattgttt
     4981 ccttctgtag cagagatcgg aatcacttgt gcaaaaggca tctgcgtcga ataatcttcg
     5041 atgattccca gcaactcgtc tggatggatc ttatcgattt tattgataat cagataaact
     5101 ggagaatgca cgttttttag acgttcaata ataaaatcgt ctccccggcc gcgtttttga
     5161 tcagcactga tcataaataa gactgcatcc acttctttca acgcactgta agctgcttct
     5221 accataaaat ctcctagacg atgctttggt ttatggattc ctggtgtatc gataaaaatc
     5281 agctgagctt ctggtgtagt atatactcct tggattttat ttctagttgt ctgtgccttg
     5341 tcactcataa tggcaatttt ttgacccacg attcggttca ataatgtgga ttttcctaca
     5401 ttcgggcggc cgacgattgc gacaaaccct gatttatgtt ctggcatata cgttctcctt
     5461 tttaattaaa caacaaatga tatattttag ggacaaataa aatcaaccca atgatgacag
     5521 caaaacaagc ggtcactaaa actgctccag ctgccatatc tttgattttt ttcccaatcg
     5581 gatgaaaatg aaaatccgta aacatgtcca ctacattttc aaaaacagta ttgatgatct
     5641 ctactatcca aacgagaaaa acagctaaaa gcagccataa ccactctatc cgatcaagct
     5701 ggaaaatcac gcccataaat aaagcaagga taccgaacgc aacatgtttc ctcatattgc
     5761 gttcttcctc aaaaacggtt ttgactcctt ggacagcaaa ttccacagac atcaggaaat
     5821 gtttgttttt ctctacgttt ttatcttttg agtccatagg catccaagat ctctttctgt
     5881 aaaccaaaca tttctttctc atcttctggt gtcatatggt catacccgtt gatatgcaag
     5941 aatccatgta ccgctaaaaa gccaagttcc cgatcatatg aatggccgta agcttctgct
     6001 tgttcagcag cacgttcaat agaaatcatg atgtctccta gtacacgagg caaaggattc
     6061 tctccatctt caaaaatcac tgggatttcg tcttcgcctt cttcttccag agcaaaactg
     6121 atgacgtctg tcggtgcatc ttttccacgg taatcacgat tgattacttg gattgccgca
     6181 ttgtccatga aagtaacaga catttctgtt tcctctggca gctttaaata agatgctgca
     6241 aattgcagca agtcatcgat ttctttcatt ttctcttctg agacttggtt tgtttcatcc
     6301 ataaaggtaa tatccattat acatcgcttc tttctattct tcttgttctt cctttatctt
     6361 ctcaacttct gttttcattt taggatattt gatccgagaa tggaaggtgc tacttaaacc
     6421 gctgatcaat gatttttcca cttttcggat ttctttcaag gtcagtccac tatcgtctaa
     6481 ttgaccatcg gaaatacgct cttcgataag attatggaca aaactttcga ttttttcgct
     6541 cgtcggatga tccattgccc gtactgcagc ctcacaactg tcagcaatat tgacgatccc
     6601 tgcttcacga ctttgcggtt ttggtccagg ataacggaat tgctcttctg ttacttccgg
     6661 attgcgttct ttcgctttga tgtagaaata acgcattagc gtggttccat gatgctgttt
     6721 gcaaatatcg atcaccattt gcggcatatt ataatcttcc aatattttgg ctccatctgt
     6781 cacatgtcca aagataatat gtttactgtc ttctggcaat aagaaattgt gcgggttttc
     6841 cgctcctgaa ggcaagttct caacgaaaaa gctagcatgt ttgatcttgc cgatatcatg
     6901 ataatagcaa gctactcgag ttagcaaaga acgtccgccg atttctgcta cagcgttagc
     6961 acttagatta gctaccatca tactatgatg ataagtacct ggcgcttctt ccagcaactg
     7021 tttcaataaa ggatgatttg gattgctgag ttcatttaat acaattacac tgtcatcatt
     7081 gaccatcaat tcaatatatg ggtgtagacc gatcgtcagc aagaatgaga agagtgcacc
     7141 agtgaacccg cagacaagca ttaaccaagt cgttgcatca ccaaagctca ttccttgata
     7201 gacgatcaaa atgacatcca tcatcacagg aaaaataatg acccacatgg cagcagataa
     7261 tccttgttct gagatccgtt tgcgtctgac aatcgttgcc atgagcccag agaaaacata
     7321 agacatcaag ataatcgtta gaaagttcgt gccaatcgaa tcataaaaaa tgaaaagtgc
     7381 agatactgct tggaaaatac cagccatgat ccctgctctt cggttcaaga aaaagcttaa
     7441 aacaagtggt gcaaaagcag cgggataaaa cagcgggata taagtagctt gttccgtttg
     7501 gaataattgg aaaaatttca tcagcactat gctgactgac atagctgtca catagaaaag
     7561 cacgtatttt gttctttctc ctgcattttg gaattgaagt gaattataaa tcagtacgcc
     7621 gatttgaagc aaaacagcta aaaccatggc aatgagtggg aaaatcgaag tcgtttgatt
     7681 cgtcaatcct agtaattcca attttttcat cgctgaagca tcaatttgat ttccttcgcg
     7741 aacaatgatt tctccttgga agatcatcac tggttgtaca gcttctcgtg cagattgttt
     7801 taattcttct gtctttttct cattcaatac atcattgact acaatgcctt gatccactaa
     7861 gtagcgaatc atttgttgct gctctgttgt gacattcaaa tactgaatct gatcttctgc
     7921 ttcttgttta aaagcttcca attcgctttc acgaacttgt ttactcatct gttcgtcaat
     7981 gagcttcaaa ctttcatccc taaccgtgtg aatttccgtt tcagtcattt caaatatcgt
     8041 cgaataaaaa ttgttcggca gtttttggta aaacgcaacg ttttctgcat tcacgttttc
     8101 aaaatttttc ttcagtgtag caatccgctc ttctgtagta ggtttcggga tattttcatc
     8161 ttccttcgcc ttatctacct tgtcattata agatttgttg atggtatcat tcgtcttatc
     8221 gatcaattcg aataattgct tgatccgatt gttctggtca tctgccagat ctttttgata
     8281 agtatattct ggagtcactg cttctgctgc cagttttctt ctttgttcgg tttcttccgt
     8341 gttctcgatc gttttattcg cacggatact ctcttctgct acttggcctt ctctgaaatc
     8401 attggccttt tgtttgacac tactaaatgt aacgccgcag acaatgataa aaaacaagaa
     8461 cagaaggaaa ggcaatgctg cctttccgag tttttcttgt agtttctgca gtgttttatt
     8521 caacatgtgc ttatcctcct aagcaaagct tattcttgat gaagatccgc cttttcataa
     8581 gctttgatga tttcggcaac tacagggtgc cgaacaacgt cacttgcttc aaaattaaca
     8641 aaatcgattt ttttgatttg ctttagtgtt cgttctgcat gaacaagacc acttgtcgtc
     8701 cctcgaggca agtctatctg acttgtatcc ccattgacaa tcatttttga ttgataaccg
     8761 agacgggtca agaacatttt catctgagcg actgtcgtgt tttgtgcttc gtccaagata
     8821 acgaatgcat catccaatgt ccgcccccgc atgtaagcta atggtgcgat ttcgattacg
     8881 cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa tttggtataa agcatcataa
     8941 acaggccgca gataaggatc cactttttct ttcagatccc cgggtagaaa gcctaggttt
     9001 tcccctgcct ctacagcggg ccgcgtcaaa atgatttttt gcacttcgcc tttttttagg
     9061 gcagcaattg ctaatactac ggcaagaaac gttttccctg ttccggctgg ccctacacca
     9121 aatacgatgt catgtttggc taccgattca acgtatttct tttgaccgct gttttttacc
     9181 cgtataggtt ttccattacg gtctttgacg atctcttcct catacatctc aacaaaataa
     9241 tctaatgtac ctttttgccc cattttcaat gccgtcacga catctggtgt acttacgtgg
     9301 attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg aggcagccaa ggaaatctgg
     9361 gattgttcac caatgatctg aatgatttct ccacgtgtat ggatggttgt ttccgttgtt
     9421 tcctcgatca gttttatatg cttgtcgtgt gcacctaaaa gcatgctgac atcatcagca
     9481 gctgtcaatc taatctctaa agaactagac tcttccgtca aaaatagaca tccccttttt
     9541 ttcgattcat tctttcctat tttaccatac tcagaagaaa ctatatagca aataagaaaa
     9601 ggttgtaaaa gagtcgttta accatacgta ccaagaaacg ttaaaaaata gctgtttcca
     9661 ttcgctaaag gtctcgtttt gataaaaaga atctgagaat gtaagttata tgaaagtgga
     9721 gaacaaaata ttccgtttca ttcagaggta ttctctaggt atttttcttg tttcaaaaaa
     9781 agaaaacaaa atagttaaaa tattcttgca tttcaaaaaa aacaggtgta ttattacgtc
     9841 aacaaataaa catacagcaa agcgtcgagg aaaacagtag gctattcaac gatttattcc
     9901 cagtgagtca tgtaagtgag aaatgacaat aaatcgaata gacgaacatc atttccgagt
     9961 caatgtcgaa aacgtagatc attgtggctg cctccattat caggctagcg gataacttta
    10021 tcaaaaagcg tccgtgaatg aagaagcttt tttaagcgaa aaaaggtggt accgttctag
    10081 aatctagagc ccttttccca agtggaaaag ggcttttttt tatattttaa ggtggtggtt
    10141 ttcatggaat ctgatattag tggcattact aattgctgca acgtttatat cgtcaatcaa
    10201 aatgataatt attggaggaa aaatttatgg aagaaacaaa aacttattca tggaaacaac
    10261 tattgatggt cagttcactt atcttcggga tgtttttcgg gtcaggtaat ctaatctttc
    10321 ctgtccactt aggtcaaatg gctggcgcaa actgggtcag tgctggcgta ggatttgcca
    10381 tctcaggcgc tcttttccca ttactagcta tcttagctgt agtagtgaca aaaagtgatg
    10441 gtctgtatga cttagctaaa cctgtcggaa aatggtatgc tgccttgttc ttaatccttg
    10501 ttcatttgac gatcggacca tttttcggta caccacggac agctgctact gcttttgaaa
    10561 tggctgctaa accatttttg cctgaaaaat ataattcatt aggtatgttt ttattctctg
    10621 ctcttttctt tttacttgct tatttcctta ctgtccatcc cacacggttg gtcaaatacg
    10681 tcggaaagta tctaaactcc ttgtttcttg cattattagc aatcattttt gtcgtttctt
    10741 tattccaccc aatgggaaat ctaaatcaat ctgctgcaga tacttaccaa acaaacgctt
    10801 taacaaatgg tgtcctagaa ggttataaca cggttgatgc agtagccttg cttgccttaa
    10861 gtgtcacttt tgtccatgca gtccgtgacc ttggtttcaa agataaaaaa gtcacagaat
    10921 taacagcaaa agctggtagc ctgagcgttg cgctagaagt catgatctat tttggtcttg
    10981 ttcttctcgg tgcgatgagt ttgaataaga tgtctttatc agaaaatggc ggaactgcac
    11041 tttctcagat cactaatttt tatttaggta acttcggcgc tagcttctta ggagtgatgg
    11101 tcacattagg tgtcttcact accgctatgg gactcgttgt ttcttttgca caagatttcc
    11161 ataaactgtt ccctaaagtc agttatatga catggctgcg gttaacgact ttcgtctctt
    11221 ttgtcgttgc aaatgctggt ttggacaata tcatccaatg gtctttacca gtattgatgc
    11281 ttttgtatcc attgtcgctt gcgttgattt tgctttcctt aacagcaaaa tttttccaaa
    11341 agacaccatt tgtctatcaa gtaacgatgt tgtttgctgc tgttcccgcc gtattggata
    11401 tgctggcaaa ttcgcctgct ttagtgagtc aacaaagagt tgtagcaagc atgctcgaat
    11461 tttatcatca tcacgttcct tttgctgcat tgggacttgg ctggatggtc ccaacgttac
    11521 ttgggtatgc tggcagctta ctcttctatt acgcctatcg cctatcaggg tataaacaag
    11581 aagcaaatga actaccagaa gaataaccag atcaaacaca atttaacata agaaatatta
    11641 aacaggaaaa aaacagctag atgaaagaat cctattcttt cgtctagcta ttttttagat
    11701 ctctttttta cgagcacaaa aaaactgcgg acaatgattg tccgcagttt aaatgataaa
    11761 attaagattg taatagttct ttcacaatgg catttacttg gtttccatct gcttttcctt
    11821 tgactttagg catgatcgca cccatgactt tgccaaattc tttcattgat gaagcaccag
    11881 tttgcgtgat tgcttcttga acaagttgtc gaatttcttc gtcggaaagt tgttccggca
    11941 tgtatttttc gactatcaca atttcacttt ttactttttc agctaaatcg tcacgtcctg
    12001 cttcttcaaa ctcatgcaaa gaatcacgtc gctgtttcat ttcgcgagac aacaccgtta
    12061 attcttcttc ttctgtcaag tcgtgacctt ctttaatctg ctcgttttgt atagatgact
    12121 tgatcattcg gatgacttgt aaagattcct tatctttcgc tttcatcgct gttttcatgt
    12181 catcgtttaa tctacttagt agtgacacac taccaactcc aattcatcac agaactagaa
    12241 ttttttgcgt tttctagctg cttcagattt tttcttgcgt cttacacttg gtttttcgta
    12301 gaattcacgt ttacgagatt cttgtaaagt acccgctttt gacacggaac gtttgaagcg
    12361 acgaagagca tcgtcaagtg attcgttttt gcgaacgact gtttttgaca tgttaattcc
    12421 ctccctccga gcttaaaaag taaccgtaat tgttattgaa atatatctca aaaaacaaga
    12481 ctgtcctgta aatattatat ctaatgctgt tttgaccgtc aaggaaatct tgcttttttt
    12541 taaaattatc aaatgttatt ttatttaaat ccttattgac aattttcaca tcttccgaaa
    12601 atctcgaatc tatgcccttc gatcgtacat ccaccgagct gttcttcgaa ataattcatt
    12661 ggacacatat ggatttcttt cgttgttccg cacaccgtac agataaagtg atggtgatgg
    12721 ccgatttccc cttgacagca atgaaaacga aacttcatct ctccatttaa atcggtttct
    12781 tccagtaaat cgatttctgc aaaatcatgg agatttcgat aaatcgtatc gtaacttact
    12841 cctttgaatt cctgattcat atagtcaaaa acttctttag cggatacgta gcgattgcgc
    12901 ttcactaaat aagttaacaa tgcttctctt tttttagtat atttaaaacc gttatttttc
    12961 aatgtttcta gtgattgttc gatcaacgtc tgttttttca tgacttattc ctccaaatca
    13021 aaatgattac gaagtacaca ttctagagta tggtataata aacataaaaa aaatgcaagt
    13081 gaggttttat ccatgtcttc cgattctaca gaaatcgtta ctttttttgt catctccgac
    13141 tctgctggag aaactgcgac aaaactagcg caagccacta tggcccaata tcctagcgtt
    13201 gagttcaatt tattccgcag aacatttgtg acagatgaga aaacattgat gcaggcactg
    13261 caagatgcac tagaagaaaa agctcttgtc ctgcacacat tgatcaatca agaattgatc
    13321 gagatgacgc gttctttttg tgaagaacat caattattta gttttgatgt gatgactccg
    13381 ccgatatcag aaatcgagcg cttaactggt atcaagccca ttcggcaacc tggcgccctt
    13441 catttattga atgagaacta tttcaaacgg atcaaagcaa tggaatttgc tgtaaaatac
    13501 gatgacggga aagatcctcg tgggtttcta gaagcagatg tcgttttgct aggcgtatcc
    13561 cgaacttcca aaacgccttt gagcttgttt ttagctaaca aaaatttaaa agttgccaat
    13621 ctacctttga tcccacaagc acatattccc aaacagcttt gggagatcga tcctaaaaaa
    13681 atcgtgggac tgacgaataa cccagatatt ttaaacaata tccgtaaaga acggatgcgc
    13741 tcttacggat taaatcctga tactgcttat tccgatatcg aaaaaatacg agctgaactc
    13801 gattttgcga atgatcttta tgaaaaattg ggctgcgtag taatcaatgt cgcttcttta
    13861 tctattgaag aaacagcttc gcttattttg aatgctttag acctagaaga ccatagttat
    13921 tatggaactg aaaccaccga agaaacagac taacaatttt tctttaaaaa aactggactg
    13981 tgtcagcgaa gaagattcgt tgacacagtc cagtttgtct ctatttgcac ctatcgttat
    14041 tgatgttcca catgtttgag tgtctggttc aaaatatcca gtacgtgaga atcatcaaga
    14101 ctatacaaaa tcgcttttcc ctcacgatgg gacttgacga catggttttc tcttaataaa
    14161 cgcagttgat gcgatacagc agattgttcc atttccactg cttggatgat tgctgtcaca
    14221 ttttgttccc cgtctttcag atacagcaga attttgagac gagtggggtc actcaacatt
    14281 ttgaatagtt ggctgacttt ctctactttt gttttttgcg tgtctggcaa tccattcacc
    14341 tccaacttga aaaaagactg acttctaaag tttaccactt tttccatcag tctcaagttt
    14401 ttttattctt ctgtcatctt ttggataatc gtttcataag cggctacttt ttcagctgcg
    14461 tcttctgaag agtttccttt tacaccgata taaaatttga ttttcggctc agttcctgaa
    14521 ggacggatag cgatccagcc attttcttcc agcgtatact tcaatacgtc agatggcggt
    14581 gtcgttagtt tttctacgtt tccttcacgg tctgttctcg tcaactcttt gaagtcttct
    14641 gtttggatga cttttgctcc ggcaaattct gttggtgctt cttcacggaa tttttccatc
    14701 aaagcagaga ttttcgcaga accttcttgt ccgctcattg tcacagaaat cgttttttct
    14761 gcgtagtagc cataggtttc aaaaatcgtt tgcaagccat catacaacgt tttgccttgt
    14821 tttttatagt aagctgctac ttctgctaat aagagcaatg cttggatcgc gtctttgtcg
    14881 cgaacgaaag gtttaactaa ataaccgtaa ctttcttcga atccgaacat aaaagtatgg
    14941 gaatgatctt cttcaaattg ttgaattttt tcagcaatga atttgaatcc tgtcaataca
    15001 ttgaacatgg tagccccata gtgttccgca attgctgtag gtaattcgct agaaacgatc
    15061 gatttcaata ccgctgcgtt ttcaggtaaa gtgcctcgcg attggtgtgc ttccaaaata
    15121 tattgagcaa acagtgctcc gatttggttt ccggtcaata cttcatagga gccatttggc
    15181 aaacgaacgg ctgcacctaa acggtctgca tcaggatctg ttgcaatcag caaatcagcg
    15241 ccttctttct cgccaagtcg tatcgcatac tcaaaagcag agtgttcttc tggatttggt
    15301 gatttgactg tagtgaaatc tgggtctgcg acagcttgtt ctggaactag tacgaattgt
    15361 tcgaatccag cttgtttcaa cgcacgttcg cccaacattt ttcctgttcc gtgaagtggt
    15421 gtgtacacaa gtttcaaatc tttccccatt gtttccacca attcatgatc aatcgttact
    15481 gctttgattt cttctagata agcggcatcc acttcttctc cgatgatcgt gatcaatccg
    15541 ctatgttcag cttcttcgtc agataatact tcaactttta acggattgct gacttcacgg
    15601 acatatttcg tcaatgcatc tgcgtctgca ggaggcattt gtcccccatc ttctccgtag
    15661 actttatatc cgttgtaagc tgctggattg tgggatgcgg ttaccatgat tcccgcaaac
    15721 gtcttcaaat gacggactgc aaaagaaagc tctggtgttg gacgtagact ttcgaataca
    15781 taagatggga tattgtgatg tgctaatgtt ttggctgctt ccatggcaaa ttctggtgat
    15841 tgatggcgtg agtcataagc gatagcgaca cctcggcgtt ttgtttcttc gccttgtgta
    15901 tccatgaaac gtgcaagtcc ttctgttgct tgtcttaccg tatagatgtt cattcgattg
    15961 atcccaggcc ctaagattcc ccgcatgcct gcagtaccga attccaacgg tgcataaaaa
    16021 gcatcttcaa gtttttcagg atcttcaccc agatcattta attgtttttt taagttttct
    16081 tctaaatttt cttcatgtac ccattgttca tacacttgtt cccaagacat gaaaataaca
    16141 cctctttctg aaatatggtt cttaataagt atagcattca cttattagat atgaaagtat
    16201 gaggaccaaa aaacgcttaa atcaagaaag gattctttta tcaagcacgg ttgcaagtat
    16261 tcgcataaat cggattcagt agatttcacg cgaaaaaaaa gagtaaaaga acaattaaac
    16321 cgttcttcca ctctttttaa acttatgcag ttgtttcttc taaagattcc aagtatttat
    16381 acacttgttg tccagcgatc ccaccatcac caacagcagt agtgatttgt cgcaaatctt
    16441 tttcacgaac atctccgatg gcaaaaacgc ctggtatatt cgtcttcatt tcgttgtctg
    16501 ttggaatcca gccagcttcg ttcgtcaacc ctgcttctct aaatggtgca gttaatggat
    16561 ccagtccaac atagatgaat actccgtctg ctgccaaatc aaactcattg ccggttttga
    16621 cattacgtgc tttcacacct gtcactacca tttcattccc aacgatttcg tcaacgactg
    16681 tatcccacat aaaggaaatt ttttcattcg caaatgcacg gtcttggatg atcttttgtg
    16741 cacgtaattc atctctacgg tggacgatga ctacttcaga agcaaattgt gtcaaataga
    16801 tggcttcttc tactgctgag tctcctcctc cgatcactac tagacgtttg ttacggaaaa
    16861 acgcaccatc acaaactgca cagtaagaaa cgccacgtcc agcaaattct tcctcgccag
    16921 gtacgcctaa tttacggtgg atacagcctg ttgcgataat gactgttttt ccttcgtaag
    16981 ttttatcttc acaaataact tctttgtggc tgccgtgatc cttgatatct tgcacgattc
    17041 cataagcatt ttctgtccca aatcttgaaa caccttcata catcttataa gctagatcag
    17101 gacccattat tgaatcgaaa ccaggatagt tttcgatttc tgccgtattg ttcatttggc
    17161 cgccaggggc gccttgttcg atcatcaata cggataagtt tgaacgtgaa gcatataaag
    17221 cagcagtcat cccagcagga cctgcgccaa taacgattac atcatacata tgtagattct
    17281 cctttactta atcaagtaag catactttac agccaatgcg aaaaaaagtc tattgttctg
    17341 cttgcgtttc tcgaatagct ttattatact cgaaaaatcc gctgaaaaac aatagacttt
    17401 tataaatcat tgatcaatca ttcgaaagat tttctccatt tgaatcaatt acttttttat
    17461 accagttgaa tgatttcttc ttcgaacggg ctaatgtgcc atttccttcg ttgtcacgat
    17521 cgacataaat gaatccgtac cttttcttca tctcacctgt accagctgat actaaatcaa
    17581 tacatcccca agtcgtatag cctaatagat cgactccatc taattctact gcatctttca
    17641 ttgcctggat atgtgctgcc agatagtcga ttcgatagtc atcttccaca taaccattct
    17701 catcgggcgt atcgactgca cctaagccgt tttctacaat aaatagtggt ttttgataac
    17761 gatcataaat atcattcatc gttattctca agcctaatgg atcaatttgc caaccccatt
    17821 cacttgcttc cagatatgga tttttgacag aagcaaagat atttcctgca gtctgttcca
    17881 agagttcgtt gtcagtagtc gaaactcgag aagaataata agaaaaggag ataaaatcaa
    17941 cagtatgttc tttcagcaat gcggcatccc caggttccat tttgatctga atgccatttc
    18001 gttccatttc tttcaatgca taagcaggat attctcctcg tgactggaca tcaatgaaga
    18061 aataattttc tcgatctgat tttctagaag cccaaacatc ttcaggattg caagtgtatg
    18121 gataatatgc tcctgctgcc agcatacagc ctactttgtt ctccggatct acttcatgag
    18181 caatcttcgt tgcgatggca cttgcaacca actcatgatg tgccgcttga tatttgattt
    18241 gttcttcatt ttctccttct tcaaaataca gccctgcgcc catgaatggt gcatgaagga
    18301 tcatattgat ctcattgaat gtcaaccaat atttgacaag tcctttatag cgattgaaga
    18361 tgactcggca cagattttca taaaaaccaa cgagttctct ggaacgccac gcaccatatt
    18421 ctttgatcaa atgcatcgga caatcaaaat gtgtaatcgt cacaagcggc tcgatcccgt
    18481 acttttgaca ttctttaaag acatcctcat aaaatttcaa accagattca tttggctctt
    18541 tttcatcgcc cattggaaaa atacgactcc aagcaatgga catccgataa gtcttgaacc
    18601 ccatttctgc aaataaagca atatcttcct tataatgatg atacatatcg atcgcaattt
    18661 ttgctggata aaaatgattt tcatcaaatt caaacatttt cttttttcca gtaatgacag
    18721 gaaaacggtc ttctccgatt ggcacaacat ctacgttcgc taacccacgt ccgccttcat
    18781 cataaccgcc ctcgcattga ttggctgctg tagctccgcc ccataaaaaa tcttttctga
    18841 atcccatcat acttcctcca ttacatgtgt attgcttgat aattctcttg ccagttcatc
    18901 ttggacttcg attccttctg cttttaaccg gctgattgtt tccttgaatt gcggtaaata
    18961 ctgctgatgt gcaataaaca actcattcat gatccgtttt gctgtctttc ctgatcgtat
    19021 aagcggattg atcgtaaacg cttgtaaagc tgttccataa tcaccagtca cagctgcttc
    19081 gatcgtcagc aattccatat tcttcattac ttgcaaccag cctctttcag cagcaggaag
    19141 ggaaccaaac gctacatttc gtgcgccttg tgcgccaaca taggcagtga cttctactac
    19201 acaatctgcc ggcagatcag gaacagctcc atcattcttt gtagatacca cgatctgtgt
    19261 ttctttattt ccatagatcg aagcaatcgt ttcacaggca gcatctgaat aataagcacc
    19321 acctcgtttt tgcaattgtt caggtttatg atccaattct ggatctttgt ataattcaaa
    19381 cagctctgct tctgtttctt tcacttgctg cgctcttgta ccaatcgttt gatattcttc
    19441 taaagcatgt tccagcattt cttcttcacg gtagtaatag cggtggtagc cacaagggat
    19501 catgttcatt tgttttaatt gttctttgaa aaacgggata tcaaagatat ttttcggcaa
    19561 tccattgtct tcatcgtata aacgatcaat caattctgcc gtgcggtcat ttcctttatt
    19621 atctgctact ttgtgccaat ggaagtgatt tagaccggca aacttgtaga tcagctcttc
    19681 ttttttcttg ccaagcattg ttggttctgt catcatcgcc attactggca cattgcataa
    19741 tcctatcact ttatcccatt tgccatatct gataactgct tctgtcacca tcccgcttgg
    19801 gttcgcgaaa ttgatcagcc aagcatttgg acataaacgc ttcatgtctt caacgattgc
    19861 caaaaccact ggaacggtac gaaaagcttt gaatatcccg ccagcaccat ttgtttcttg
    19921 cccaatcatc ccataataac tagggatccg ctcgtctttg attcgtgcat ctaattgacc
    19981 gacacgaaac tgagtcgtaa caaaatccgc atctttcaat gcttcttccc gatccagagt
    20041 tgcatagatt ttgacttcat acggagaagc atcccacata cgctgagaca tctgactgac
    20101 gatctcaagc ttttcttttc cttcttctac atctacgagc cagatttctc gtatcggtaa
    20161 ttcctcatat cttttgataa atccttccat caactcgggg gtgtaactgc tgcctccacc
    20221 aatcgtagcg attttgattc cttttgccat gttctcactc ctttactttt tacactttta
    20281 ttatcttttt tgtaaacaaa acaaacaata gaaattcgtt tctttccagt aacatttaca
    20341 aaagttagtg ttttctactg gatttttgta aatatcttgt caaaaaatgt ctttattaag
    20401 ctatataaca taaatccgtt tacaaatcat tttcaaaaaa gttataattt acgtaaaaaa
    20461 acaagaagaa ggtatctaca tgttattacg tgaaaaaatc aaagaaatct ccttttctcc
    20521 ttcagaaaat gaagtgattc agtttctctt aagcgattct ccagcacttg gcgaaatgac
    20581 catccaagag attgctgagc gatgttatgt ccacccgtcc acactcatcc gtgtagcaaa
    20641 aaaaatgggt tttgatggct gggtcagttt tcgtaatgct tttttagctg aaacagccta
    20701 tcttgaaggg aattttcaag acgtagatgc gaatcttcct ttttccacca cagacggtat
    20761 tatgacgatc gcaaataaaa tggcttcttt agaacagatg acgattcaag atacactttc
    20821 tctcattcat catgaccaac tgcaaaaagc caaacaacta atgcttcaag caaaaaaaat
    20881 cgtgattttc gccagtaact cgaatacttt gatttctcaa gatttcatgc tgaaaatgaa
    20941 gcgcatcaaa atggatgtca cgattgtcac cacttttggt gaatcgttct atgaagcgta
    21001 caattgcacg agtgatatgt gcgggattct gatttcttat acgggtgaaa acaatgtgat
    21061 ccttcgtacc gcagaaatat tgaaacaaaa tcatgttcca attttagctt tgaccagtat
    21121 cggtgattca acgctatctt caatgagtac agccgctttg cgacttacga cacgcgaacg
    21181 actgtattcg aaaattggta attttacgat caatacatcg atttgttatc ttctagacgt
    21241 gttatatagt tgtgtttttg cagaaaatta tcaacagaat ttagtccatc taatttctgt
    21301 aggacaaaaa tttgataaac gccctatcag ctcagccatc atgaaagagc ctttcaattt
    21361 tgaaaataaa gaagtggact aaaaagaata attaagccta gtccattctt tcacctaacc
    21421 gttcaggtca gtgcggtgat aagtgcatcg tctggcccga ccgtctcccc ttgattttcc
    21481 aaaatatcca aataatccgt gtaattggtg acgatcacag gtgtttctaa actgtatcct
    21541 tcctcttcga ttttcttttt gtcgaagcgg agcaacagat cgccttgttt cactctttct
    21601 ccttgctgca ccaagctttc aaaatatttc ccttccaact gaacagtatc caatccgaca
    21661 tgaatcagca attccagacc tgcttcagaa accattccga ttgcatgttt cgtcgggaac
    21721 agggtcatga ctgttccgtc aaaaggggca cgtacttctc ctttttcagg gtagatcaag
    21781 attcctcgac ctagtgttcc ttgtgcaaaa gctgcatcct ttgctgcatt cagtggagcg
    21841 atttgtcctt gtatcggtgt ttttacaatt tctttacctg atcttttctg tctatcatct
    21901 gtttcacctg cttcctcctt ccaaaagaaa aaagtcagcg caaaggatac caccatcgca
    21961 atggcgattc caatcaatac attaatcaca tccgttaaat caccatctgg tctaatatag
    22021 ttgggaattg taaagatgcc taatccacct ggattccaag aagttaaatt cattaatcca
    22081 ctatacgcgc cccctaacgc accaccgaca caggtaaaaa taaatatttt tttacgtggc
    22141 aatgtaaacc cataaatagc tggctctgta atccccacaa taccagaaac aatggctggt
    22201 attgcgattg ctttgtcttt tgggtttcta agcttaaagt agagtgcagt aagtgctgca
    22261 gtttgtccaa aagacactac tgcaacaggt gtcatgatat tttgccaacc attcacagaa
    22321 aattgcatca atcccattgg tacgattgcc caatggagac caaacatcac tagaatttgc
    22381 cagaagaagc ctacgattgc tccataaata actggcgaaa gtgctaaaag tgtttcaaat
    22441 ccagccatta aaatatcagt tgcgaatgtg aatgctggtc caacgagtaa aaaacctaag
    22501 ggcattgtaa taagaacggt gaagaatgga acgaagaaat tcgcgatcat ctcaggtact
    22561 aactttttaa ttggtttctg aatttggcta gctaacaaca caataaagat aatgggaatg
    22621 acagaagaag cgtagttacg cgcaatccag ggaatgccga atactgtttg ataaataggt
    22681 gcttcaaaaa tagtgccaga aaatatggta gtcagtggtg cttctgctgc accactcaaa
    22741 gcttccagcg acaagctagg attcattaat gctgccccaa gcaacatccc tacgaattca
    22801 tttactttaa atttacgggc agctgttaaa ccgataataa caggcataaa ttggaataca
    22861 gaatcaccga tattatcaaa cataacaaat gtgccagaac cgcggtcaaa tgcgcctaag
    22921 aagacaagta atgctgtcag tccacgaatc atcccaccag cagctagaat agcaaggaat
    22981 ggctggaaac agcccgatat tccatcaata agcatatcaa acggtctcat tttttctgtt
    23041 gaaacagcct catcaaaagt aaggtttcct gcttttataa cctcttcaaa cacgactggc
    23101 acatgattcc cgatgacaac ttggtactgt cctcccgctc gcattaccgt cacaacaccg
    23161 tccatatttt tcaagatctc atcatttgct ttttcttcgt tttttaattt gaaacgaagc
    23221 cttgtgatac aatgtgtcaa accattgata ttttccttcc cgccgacatt ttctacaatt
    23281 ttgctcgcta attcttgata ttttcccatt tctattccct ccaatttatt agtggaaggt
    23341 ttagccaact gaatgtcact acccttattt catgcgttct tgttaattta tcctcctttt
    23401 tttatttatt tacaacgatt ctcatgcact aaacgcgcaa tatggatcgt taaatacatt
    23461 ttttcgtccg tggacatctg gtagttgaat atctcggcta aaaagtctgc tattttctca
    23521 acgcaaccaa acgtatcctt atattttttt tgaataacct ggtacaattc ctcagaaact
    23581 tctcccactt ctgatttttc tccatctagg acgcgtgtca gaaaaaaacg taggtgtgtg
    23641 acgaatcgaa aaaaatggac tgaatcttca tcaaatgtaa tcttatagta atagcggacg
    23701 atatttagaa tatcctgtat tgttgtagta aacaggtagg tattgtcttg ttggctttga
    23761 atgccttgtg cattgactaa atgcaatgcg ataaatccgg cttcatcttc tgataacttg
    23821 atgtggtatt tatccgagat tttcttcact gtttttttcc cgatggcata ctctttagga
    23881 aatagttgtt tgatatcata cagcaataca tttggtacaa atattcccat tttgtgacgt
    23941 tcgatagccg tatacaaatg atccgtcaat gcaatataca ccgaatcatt caactggact
    24001 tgtagagcct cttttgtata ttcaatgatt tcacaagaaa tttctaagta ctcaagagga
    24061 atcgcttgtg ccaattcttg gaactgttgt gaaagtccgt catttgtcaa ctgaaatttt
    24121 ttgtcgatgc atgagtcaaa aatcgggtca cccactttct ttttgaatgc caaacctttt
    24181 cccatcacga tcatttcctg atttctttca tccctggtta ttacgacgtt gttattcagt
    24241 actttctcaa tcagcatgtt ttactccttt ccatgatata tagggctgta tccaaaacaa
    24301 aaagaaaaac ccaaaaagcc gtccatggaa ccatgggaga ctcatcaggt ttagcttgca
    24361 tcaggcaatc actatccaag ttgtatcgtt ttctttatat tatcgaaagc aagcactttt
    24421 gtcaacgctt tcttagcggc tattgggcac ttctgactag ctgtcgtgat tttttttaaa
    24481 tctggtaatt acgacactaa cgctcacaga tgaagaatct gggaagctct gttttatttt
    24541 cactctagcg cttaggtcaa aatgctacta tgctactgtc ccaacttcta atatacggtg
    24601 ttccagtacc agcttatcgg tgataagcca aatttatgca aaaatctgga aagccttttt
    24661 tacaattttt ttcttatcac ctaaagctga acggagccct gtcacaacca ctttttacac
    24721 accactgctt tgcagtttgt acatatctgc atacaaacca ttttgcgcta gaagtgtttc
    24781 atgattccca cgttctacta tatgaccttt gtctaaaact aaaatcaatt ctgcatcccg
    24841 aatcgtggac agccgatggg caatcgcaat cgttgtccgt ccttgtcgca tttttgtcaa
    24901 tccttcttgg atcaatcctt ctgtttcagt atcgatattt gctgtcgctt catccaatac
    24961 tagtatttta ggatctgtca cgattgttcg cgcaaaagtg atcagctgtc gttgtccgct
    25021 agaatagctg gcaccgtgtt cgatcacttt tgagtgatat ccttttggta attcatcgat
    25081 gaacttgttt gcttgaacaa actcagctgc atggcgaatc tgttcgtctg tgatagttgg
    25141 attcatcatc cggatatttc ctgcgatatc tccataaaac aagaaggcat cttgcaagac
    25201 caatcccatt ttcttacgta actcttccat tggatattct cgaatgtcat gatcatcaat
    25261 caagatctgg ccttcataaa attcgtaaaa ccgcatcaat acgttgatga tcgagctttt
    25321 tccgcttccg gtgtgcccca ctaatgcaac ggtctctccc ggattagcga caaaagagat
    25381 attgttcaat acattgtgct ctccatcata agaaaagctt acattgcgaa actcgatctt
    25441 acctgaagta atcgcttgat ctgctccagg attttgttgc ggtgcatatt cttctgtatc
    25501 catgattttc aaaatacgac taccggcaac cattccatct gtaaaggtgc tcaaaaagtc
    25561 catcatttga gacattggat taaaaaatcc ctggacatag gtcgtaaatg catagatcag
    25621 tccaacttct acgggagaat gcagcgcatc gatcccaaac aaagtcaacg ccaacgcaat
    25681 cgccaatgca tatagaaaat tgatgatcgg acttaacaga atcgaatttg ttttgatcat
    25741 cgcatagcgc gtgtctaaat actcgttgtt cacttgttca aattctttag ctaaacgagc
    25801 ttcttggcga aactgctgga taatacgcat gccggaaatc gattcattca atttcgtgtt
    25861 cagctgactt aatttttccc gcatattccg gtaaatacga gaactgaatt tctggtaata
    25921 ccagatgacg attcctaaaa tcggcaagaa caccaaagtc cacaaggcaa tttctccatt
    25981 gatttgaaac atggcaatga aggaagaaac gactgcaaag atcccagtca ataccatcaa
    26041 aaagacatac caaaattcaa atagcgtttc tgtgtcattt gtcactcgag aaacagtcga
    26101 tcctgccggt gtctgatcaa aataacgcat acctagtgta tgcagctttt caaataatct
    26161 tacccgaaca tactgatagg tcttcaatga agccatggaa tacaaatacc attggaaaaa
    26221 ccaaatgata cttttgacta tgactccaaa caagtacata ccagcaaaaa acaaaataac
    26281 ttgtgtcgta gccgactgtt tagccagatg atcatccata aaagtttgga tgatccttgg
    26341 caataggaca ttgatcacag acagggcaaa cgcaaataag atagcaacga tgaacgttct
    26401 tctaaaaggt ttggcgaact tcatcagtcg tttgatgatc ttcttctgtt ctttaagtgg
    26461 aatcgatttt gtccattccg attgataatt ttcatccatc aggcctcgcc tccttctatc
    26521 ttcgcttcta atttctgttt ctcccacatt cgttcatacc agccgcctaa tgcaagcagc
    26581 tcatgatggg ttcctcgttc aatgattttc ccttgatcga caacaatgat ttcttttgca
    26641 tgcatcacgc tgcttaaacg gtgagctgca atgatggttg ttttctcttt tcttatttct
    26701 tttaaattat gcaagatagc ttcttctgtt ttggcatcta ctgcagaaag tgcatcatct
    26761 aagatcagca attcaggatt tttgatcaat gctcgagcaa tcgaaatcct ttgtttctgt
    26821 ccgccagata aagagactcc acgttctcca actaaggtat cataaccttt tggtaattcc
    26881 ttgatttcat tgttgataaa tgctaaagaa gccgcatgct ctacttcttc ttctgaaagt
    26941 tttggattag aaaaacggat attatctcga atggtcatcg aaaacaagaa gtgatcttga
    27001 ggcacatagc cgattgatcc taataaagca ttcaatgaat attctttgat cgaacatccg
    27061 ccaaattgaa tttttccttg ataatcatcg tattcacgca tcagtaattt gatgattgtc
    27121 gtttttcccg caccagtttt cccaacaata cctaatgttt ctccttcttg gatcgcaaat
    27181 ttgatttgtt ctaatgctgg tgattcttct ttcggatagt taaaagagga aatagctaca
    27241 ttcagctcgc cttttgccgg agtctgcact gcacccagtt gttccacgat atgcgtcttt
    27301 tcatgcaaaa gttcattgac gcgatcataa gaagcatttc ctctttccaa tacgttgaac
    27361 aatcgaccaa tcgcaaacat cggccaaaca agcataccga tatagctaat aaatgagacc
    27421 atctgcccga ttgagatcgt gccttccaag acatactttc cacctaaaat aattgttgca
    27481 acataagaca gaccgataat caaggtaatg aatggatcaa ataaggagtc taaaaaattg
    27541 actcgtttgt ttttaataat tgcatcttca atcttttctt caaaatccgc gagatcttct
    27601 ttttcttgtc caaatgtttt aatgactttg atcccagtaa tactttcttg tgtcttatca
    27661 ttgatactag aaaaagcttc ttgcgaatca cgaaaagcat cgtggagttt tgaccccagc
    27721 actcgtgaag taacggctag taaaggcaga ggaatcagcg caatcagtgt taagcgccaa
    27781 tcaataaaca aaatcatcgc aatgatcgtt gttccgcccg tgatcactga atctgcaaag
    27841 gtcaagatac cagctcctgc tacattttgg attgcattca aatcattcgt tgcatgagcc
    27901 atcaaatctc cagtccgata tttttggtaa aagagattgt ccatttctgt aaaatgtttg
    27961 aatagctgct gccgaagttc tttctccaac cgagctgcac ttccccaaat attcgtccgc
    28021 cagatataac gaaaaagata ttgtaagaga gctgcaagga tcaataataa gatccaccag
    28081 gaaatgatct ttattgataa atttttatcg gcgatctcat caatgatgat cccaatcact
    28141 tttggaggag ctaactgtac caaggccact gcgaataggg agaaaatccc aattaaataa
    28201 tgtttctttt cctgtcgaaa aaaccaacct aattttctaa aaatcgacat tttactcctc
    28261 ctttgtcttt atcaagagca taggattgtc tgtgcttgtc ttttttgctg aatagtctga
    28321 atagtctaac aaattacgaa ataaaaaacg gactgaaaaa atcagcccgc aaaaagtctt
    28381 tgggtcagaa cctatttttt cccttggttc ttcatttgct gcatcatctg gcgaattttc
    28441 ttctcagatg gtttttgccc cattgacatc atcattgatc gcaacatgtc ctcattaacg
    28501 ggaggatttt tttctaaata gtctttcata tatttacgcg ctaggaaaaa gccacctact
    28561 gcgccgatta gcaaagcaat aatagcaata agtacgacaa tacctgttga taccatataa
    28621 atctctcctt tctatttccg aattgctctc aatgatttat tttactaaag tttattgaaa
    28681 aaagaaaggc gtattcttta gtttcatgca aaaaatagtg gtttttttag agaagaacaa
    28741 aatttctttc attaaccagc aagacacgcc acgaaaaaaa attaggcata ccaaatcttt
    28801 ttctttaatt ttttctttct gaggtgtaga atgtaatcgt agataaatag gaaaggtggt
    28861 tttttcttga ataatcaatc caacaaacaa tttcataagc atcgacttat tgaacataca
    28921 aatggtttat ctttagctga agtaaatgga acagtggatg tacccaaggg aaaaagtttt
    28981 tttaaaacac ttcttgctta ctctggacca ggtgcattag tagcagttgg ttatatggat
    29041 ccaggtaact ggtccacttc gatcactggt ggacaaaatt tccaatattt attgatgtca
    29101 gttattttga tgtctagtct gattgctatg cttttacaat atatggcagc aaaattagga
    29161 atcgtgaccc aaatggattt ggcccaagca atccgagcac ggacaagtaa gacattgggt
    29221 gtcattttat ggatcttgac tgaattagcc attatggcaa cagacatcgc agaagtcatt
    29281 ggtgcagcaa tcgccctcta cctgcttttt gatattccat tggtaattgc cgtatttatc
    29341 actgtgttag atgttttcgt ccttttattg ctgacaaaag tcgggtttcg taaaatcgaa
    29401 gctttggttg tctgcttgat tttcgtcatt ttattcgttt tcgtctatca agttggcttg
    29461 tctaaccctg attggcacag tttgtttgcc ggatttatcc ctaacagtca cacattctca
    29521 gactcaccgg tcatcggcgg cgaaactccg ctgactggag ctttagggat tattggtgca
    29581 acagtcatgc cgcataactt atatcttcat tcagcagttt ctcaaactcg gaagatcgat
    29641 caccaagacg aagaagatgt agcacaagcg gtacgctttt ctacttggga ttctaatatc
    29701 cagttgtcaa tggcttttct agtcaatgca ctgctattga tcatgggtgt ggctgtattc
    29761 aaaaccggtt ctgtccaaga tccttccttt tttggtctgt atgaagcttt atctgatcca
    29821 tcagctatga gcaatggtat cttgattgct gtagcaaaat ctggtatctt gtctacttta
    29881 tttgctgtag cacttcttgc ttctggccag aactctacta ttactggtac gttgaccggt
    29941 caagtcatca tggaaggctt catccatatg cgtatcccaa tttggctaag aagactagtc
    30001 acacgattgc tttctgtgat tcctgtattg atttgtgttt tgtttacaag tacaaaagga
    30061 acgatcgaag aacataccgc tttgaataac ttgatgaatg aatcccaagt ttttctggct
    30121 tttgcattgc cattctcgat gattccttta ctgatgatga ccaatagcca agcggagatg
    30181 gggagccgat tcaaaaattc atttgttgta aaactacttg gctggttctc tgtgattagt
    30241 ctgacttact tgaatttacg aggattacca ggtcaaatcg aaagtttctt cggcgagcaa
    30301 gccacctcag ctcaactagc attggctgat caaatcgctt atgtaatcat tgttcttgtc
    30361 cttctcctgt tggcatggac gattggtgat ttatataaag gaaacaaaaa atatgaacag
    30421 cgtttgcagg aaacgataga agaaatcgag tagaaactga ttcttttaca caaaggaggt
    30481 tgtgtcagaa gtgggcagct tcaagaaata agaaggaatt taagaaaatt gcttttcaaa
    30541 tttttgtgaa atttcggctt atttccgaag aagttacttc tggaacaccg tgtatcagag
    30601 gttgtgaaaa agctgccttg catcaagtaa taagaacagc caagtaaaaa cagcttttta
    30661 tgtttttaac ttggttggac ttattaccgc agatgcaagc ttttgagcac cgtttattcg
    30721 gaaaaaggaa ctgcgacaag acttttgtca cagttccctt tcctacgatt agtcaagtaa
    30781 aaggtgtaag aaaaatagct cataaacgaa agcgacaaaa tcaa
//
LOCUS       DAZKOR010000027        30516 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000027 DAZKOR010000000
VERSION     DAZKOR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.027"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..208)
                     /locus_tag="ACG8ZM_001128"
     CDS             complement(<1..208)
                     /locus_tag="ACG8ZM_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492101.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIIS"
     gene            complement(196..825)
                     /gene="pyrE"
                     /locus_tag="ACG8ZM_001129"
     CDS             complement(196..825)
                     /gene="pyrE"
                     /locus_tag="ACG8ZM_001129"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0492102.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(832..1548)
                     /gene="pyrF"
                     /locus_tag="ACG8ZM_001130"
     CDS             complement(832..1548)
                     /gene="pyrF"
                     /locus_tag="ACG8ZM_001130"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0492103.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(1545..2477)
                     /locus_tag="ACG8ZM_001131"
     CDS             complement(1545..2477)
                     /locus_tag="ACG8ZM_001131"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0492104.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(2474..3250)
                     /locus_tag="ACG8ZM_001132"
     CDS             complement(2474..3250)
                     /locus_tag="ACG8ZM_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0492105.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(3304..6489)
                     /gene="carB"
                     /locus_tag="ACG8ZM_001133"
     CDS             complement(3304..6489)
                     /gene="carB"
                     /locus_tag="ACG8ZM_001133"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0492106.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISNKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(6494..7570)
                     /locus_tag="ACG8ZM_001134"
     CDS             complement(6494..7570)
                     /locus_tag="ACG8ZM_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0492107.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(7575..8855)
                     /locus_tag="ACG8ZM_001135"
     CDS             complement(7575..8855)
                     /locus_tag="ACG8ZM_001135"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0492108.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(8874..9800)
                     /locus_tag="ACG8ZM_001136"
     CDS             complement(8874..9800)
                     /locus_tag="ACG8ZM_001136"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0492109.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(9813..11096)
                     /locus_tag="ACG8ZM_001137"
     CDS             complement(9813..11096)
                     /locus_tag="ACG8ZM_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0492110.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(11110..11649)
                     /gene="pyrR"
                     /locus_tag="ACG8ZM_001138"
     CDS             complement(11110..11649)
                     /gene="pyrR"
                     /locus_tag="ACG8ZM_001138"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0492111.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(11884..12795)
                     /locus_tag="ACG8ZM_001139"
     CDS             complement(11884..12795)
                     /locus_tag="ACG8ZM_001139"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0492112.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(12792..13256)
                     /gene="lspA"
                     /locus_tag="ACG8ZM_001140"
     CDS             complement(12792..13256)
                     /gene="lspA"
                     /locus_tag="ACG8ZM_001140"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0492113.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(13261..13959)
                     /locus_tag="ACG8ZM_001141"
     CDS             complement(13261..13959)
                     /locus_tag="ACG8ZM_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0492114.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(13946..14332)
                     /locus_tag="ACG8ZM_001142"
     CDS             complement(13946..14332)
                     /locus_tag="ACG8ZM_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492115.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(14479..16149)
                     /locus_tag="ACG8ZM_001143"
     CDS             complement(14479..16149)
                     /locus_tag="ACG8ZM_001143"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0492116.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            16332..16565
                     /locus_tag="ACG8ZM_001144"
     CDS             16332..16565
                     /locus_tag="ACG8ZM_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0492117.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(16664..17095)
                     /locus_tag="ACG8ZM_001145"
     CDS             complement(16664..17095)
                     /locus_tag="ACG8ZM_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0492118.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            17174..17587
                     /locus_tag="ACG8ZM_001146"
     CDS             17174..17587
                     /locus_tag="ACG8ZM_001146"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0492119.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(17625..18773)
                     /locus_tag="ACG8ZM_001147"
     CDS             complement(17625..18773)
                     /locus_tag="ACG8ZM_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0492120.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(18895..19491)
                     /locus_tag="ACG8ZM_001148"
     CDS             complement(18895..19491)
                     /locus_tag="ACG8ZM_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0492121.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(19691..22333)
                     /gene="alaS"
                     /locus_tag="ACG8ZM_001149"
     CDS             complement(19691..22333)
                     /gene="alaS"
                     /locus_tag="ACG8ZM_001149"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0492122.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            22721..23152
                     /locus_tag="ACG8ZM_001150"
     CDS             22721..23152
                     /locus_tag="ACG8ZM_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0492123.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(23197..24546)
                     /locus_tag="ACG8ZM_001151"
     CDS             complement(23197..24546)
                     /locus_tag="ACG8ZM_001151"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0492124.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24616..25779)
                     /locus_tag="ACG8ZM_001152"
     CDS             complement(24616..25779)
                     /locus_tag="ACG8ZM_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0492125.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            25908..26231
                     /locus_tag="ACG8ZM_001153"
     CDS             25908..26231
                     /locus_tag="ACG8ZM_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492126.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(26414..27763)
                     /locus_tag="ACG8ZM_001154"
     CDS             complement(26414..27763)
                     /locus_tag="ACG8ZM_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0492127.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(27832..28785)
                     /locus_tag="ACG8ZM_001155"
     CDS             complement(27832..28785)
                     /locus_tag="ACG8ZM_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0492128.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(28887..30077)
                     /locus_tag="ACG8ZM_001156"
     CDS             complement(28887..30077)
                     /locus_tag="ACG8ZM_001156"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0492129.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(30277..>30516)
                     /locus_tag="ACG8ZM_001157"
     CDS             complement(30277..>30516)
                     /locus_tag="ACG8ZM_001157"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492130.1"
                     /translation="KNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVFL
                     LVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         8576 a         6691 c         5353 g         9896 t
ORIGIN      
        1 aagagattat tctcttcttt ctgctaacca gtcttagaaa aatgaatttt aaaaaaatca
       61 aaaactgatc acgatagaag tttcttttca tacctgatga aaagatgctt ctcgcaatca
      121 gtttttggat ttttgctatt ccaattgatg attctggaga cacaaatcgg atttctctat
      181 cttcatcaag cgcgcttaaa tctgccaatt ttcaggatct tttttccatt cttttagcag
      241 agttaattct tgctcatcga tataacgatc ttctaaagct gcttcaatca atacagaata
      301 attggtcaac gtcataagag ggatttcggc tttttcgaaa tttgcttttc cttttggcaa
      361 ctcatacgta aagatagccg caacaccaag gatatccgca ccttctcttt ttgctgcttc
      421 agctgcttcc aagacacttc cgcctgtaga aatcaaatcc tcgatcacaa ccatcttctg
      481 accttcaaat atacgtcctt cgatttggtt acctttccca tgatctttcg ccttacttcg
      541 aatatagacc attggcaaat ccagtatttc cgctacccat gcggcatgag ggattcctgc
      601 tgttgcagtt ccagcaatca cttgtacttc agggtaagct tctttgattt tgcttgctaa
      661 tccttttgcg atctcttttc ggacttttgg atagctcatc gtgattcggt tatcacagta
      721 aattgggctt ttgattccac ttgcccatgt aaaaggatta cttggattta aaaatactgc
      781 ttcaatttct aataagtctt ttgcgatact ttgttcaaca ttcattttag gttagctcca
      841 ttctgtttga atcgtttgat atgctttata aggattagtt gactgagtga ttggtcgacc
      901 tataacgata aaagttgagc cgatcttttt tgcctgagaa ggtgtgacta ctcttgtctg
      961 atctcctgcg actgttcctt ctggtcttat acccggagtt aagcaaacaa attcgtctcc
     1021 tgccatttct ttgatcgctt ctacttccca agcagaagaa accacaccat ctaatcctgc
     1081 ttttttggca cagctagcat aatggatcac acttttttct aaagaaactt cgattagttg
     1141 atcggcatgc atttcttctt cacttgtaga agtaagctga gtgatagcta gaagtagcgg
     1201 acgttttcca ccttctggag tcccctcttc taatcctcgc attgccgcct ccatcatacg
     1261 aataccgcca gcggcatgaa cacaggtgag atcaacacca agctttgcca atccccgcat
     1321 tgctttttcg accgtattcg gaatatcgtg aagtttcaga tctaaaaata cattatgacc
     1381 agcctctttt agataacgta cgatttcagg tccttcttgg tagaacaatt ccattccgat
     1441 tttcacaaac aatttttctt ctttcgggaa aaatcgaaga aagtcttcta cctcttgcct
     1501 agttgaaaaa tctaatgcaa taattggtcg ttggtctagt tgattcatcg ttcacgttcc
     1561 tctctcactt ctttgattaa tctttccagc gattcgatgc ctagttcgct cattcgcaca
     1621 gggagctctt taattagttt aggacagata tacggatcgg tgaaattcgc ggtccctata
     1681 gctacagcac ttgctccagc cataaacatc tcaagaacat catccactgt ctgcacgccg
     1741 cccatcccaa tgattggaag gctggatata cttgctactt ggtgaatcaa acgaatagcg
     1801 acaggcttga ttgctggtcc ggagagtcca cctgtttgat tggctaaaat cggttttctt
     1861 gtcttaaggt caatccgcat accaagaaga gtattgatca tcgtaaaccc atctgcccct
     1921 ccagcttcga ttgcctgagc gattggaacg atgtctgtta catttgggga caatttgaca
     1981 tagacaggaa cagaagaaac tttctttact gcttgtgtta attgaaaagc aacatcagaa
     2041 tctgttccaa aagcaattcc gccatgtttg acatttgggc aagaaatgtt cagctcgatt
     2101 gctttcacat ttggtgcttc gccaattttg ctacaaactt ctacatagtc ttcttcacaa
     2161 gcaccagcaa catttgcgat gatgggtaac tcattgtact cctctaattc tggaagaagt
     2221 ttatgcataa caacgtctag accaggattt tgcaaaccaa ttgcattcaa catgccagat
     2281 ggtgtttccg ccactcttgg cgtgggattg ccaaagcggg catttggagt cgttgccttg
     2341 accatgatcg atccaagctg attcaaatca tagtattttc cgtattcttt gccaaaaccg
     2401 aaacagccgc ttgccggcat gataggattt tttaattcta ggccaggaag tttgaccgct
     2461 aaaggattct cattcatata attacctctc ctattttaaa aatcggtcct tcgtcacaaa
     2521 ctttcacact ttttgttcct gtttcatctt ccggcgtatg acaaacgcaa gcatagcaag
     2581 cccctatccc acatgccatg cgggattcta atgataactg gacattttga tgcgaagaaa
     2641 acagctgttc cactgtcttc aataacccgt ttgaaccaca tgcaaaaaca gctgtaggtt
     2701 ccgtcttcgt tcctaacaat aaattaccga catttccgtg cataccgtat gttccatcgt
     2761 cagtagcgat tcttgtttct cctagacgat taaattcttt ttcataataa acgacttcat
     2821 gtgtggcaaa acctaaaaaa tgcgttggct taattccttt ttcgactaac tgttttgata
     2881 actcatatag aggagggaca ccgattcctc caccgattat aaatacttcg tctcctgtat
     2941 gaagattgga caaatcaaaa ccattgccta aaggacccat gacatctaaa tggtctccgg
     3001 cagaaagagt agagaagact ctagtcccct caccttccac ccgataaatg atacggcaag
     3061 tatttgtttc gtggtcgata tgattcaagc taatcggtcg gcgtaatagc aagtctgcac
     3121 gcggcacgag aatatgcaag aactgtcctg gttgctgcat ttcttttact agatcacctt
     3181 tcaatgtcat ttcaaaaatc tttggtgcca gctgtttttg ctgaacgatt tccatcattt
     3241 cttgtttcat ctgtcccctc ctgcaacggt ttttcgtctt tttgtcttaa catgtgctcg
     3301 ccgttaaata gggttagttg aaaaagcacg ggattccatc actcgaacga tcgcatctgc
     3361 tgtgtctaaa gaagtaaaca atggaatacc atgttcaact gcttctcttc ggatgatgaa
     3421 accgtcttta gaagcatttc cccgatcttt atcaatcgta ttgacaacca cttgtgtttt
     3481 accatctctt attaagtcga ctacattttt ttctgctgct tcctcagaaa ttttcgctac
     3541 tgtttcaacg accagaccat ttttttcgaa ataagcagct gtatgtttcg ttcctatcag
     3601 actatagcca atttctgtaa aacgtttagc taattctagt gcttcttctt ttgtttcgtc
     3661 cgcaatcgta aataatacgg ctccatattc aggcaagtgt aatccagaag cttcgaatgc
     3721 cttgtacaaa gctttgtcta attgtctgtc agatcccatt acttctccag tcgatttcat
     3781 ttcaggccct aagtaagtat caactttctg caatttcgta aaagaaaaga caggtgcttt
     3841 aacatgaact tcttttgttt ctggataaag tccgttttca taaccaagtg tctttagttt
     3901 ctctcccaaa atagctttcg tcgcaatttg tgccattgga attcctgtca ccttacttaa
     3961 gaatggaaca gtccgacttg ctcgaggatt gacttcgatc acataaacct cattttcatg
     4021 gatgacaaac tggatattca tcatcccgac acaattcaaa cctaatgcta agcggatcgt
     4081 gtaatcttcg atcgttttct tgatcgattc agataatgtt tgaggtggat acacagccat
     4141 tgaatcccct gaatggacac cagcacgctc aatatgctcc atgatccctg ggatgagtac
     4201 tgtttcaccg tcacaaatcg catccacttc gcattcacca ccaagcaagt aacggtccac
     4261 tagtactgga tgttctggag atgctttcac agcatgttcc atgtaatctt ctaaatctcg
     4321 ctggttttca acgatttcca tcgcacgtcc gcctaataca tagcttggac gaacaagaac
     4381 tggataacca attctcgaag cgataaccac ggcttcttct ttacttgtag cagtatcccc
     4441 taaaggttga ggaatatcca actctttcag cgcttgctcg aataaatcac gattttccgc
     4501 acgatccaag tcttcgatcg ttgtccctaa gatttttaca ccagcagcag tcaaaggttc
     4561 ggctaagttg attgctgtct gaccaccaaa ttgaacgatt actcctatcg gctgttctag
     4621 ctcgatcaca ttcatcacat cttcgaaggt caatggctca aaatataatt tattcgaaat
     4681 agaaaagtca gtcgaaactg tttcaggatt gctgttcatg atgattgctt cgtatcctgc
     4741 ctgctggatt gctttgacag aatgcacagt cgcatagtca aattcaaccc cttgtccgat
     4801 acggatagga ccagatccca gtactagaat tgagggcttt tctgatcgtg aactttcatt
     4861 ttcatattca taggaactat aaaaataagg tgtttttgat tcgaattccg ctgcacaagt
     4921 gtcaaccatc ttatatacag gaatcaaatc attttctttt ctcatctggc gaacggcttc
     4981 ttcgtttatt tcccacagct ccgccacttt tttgtctgta aaaccgtttt gtttcgctgt
     5041 tttcaatatc ttgtcatcca acggctgctg tttcaactgc tgttcgattt ctacgatatg
     5101 caacagttta tctaaaaaga ataaatcgat ttttgtcaaa tccgctaatt cttctatggt
     5161 gtaccctctt ctaatagctt cagctacata aaacaggcga tcatcttgtg ctcgaaccac
     5221 tttttggatt aggtcatcgt ccgtgatgtc tgccaactca ggaagttcga tatggtgtgt
     5281 accgatttct aaagaacgca cagctttcaa taaagactct tcgatattcc gaccgatagc
     5341 catcacttca ccagttgctt tcatctgcgt gcctaaaagc cgttctccgt tttcaaactt
     5401 atcaaacggc cagcgaggaa ttttagctac aacataatct aatgcaggtt caaattctgc
     5461 aaaagtcgtt cctgtaacag gatttttcat ttcgtctaat gttaatccaa gcgctatttt
     5521 agcagctagc ttagcgatcg gatagccagt cgccttactt gctaacgcag atgaacgaga
     5581 aacacgcgga ttgacttcaa tcacataata attaaaacta tgtggatcaa gagcgagttg
     5641 gacattacag cctccctcga tcttaagtgc tcgaataatc gaaagagaag catcacgaag
     5701 catttgatat tcatggtcag acaatgtttg actaggggca aatacgatag aatcacctgt
     5761 atggataccc accgggtcaa aattttccat attgcagaca acgattgcat tatcagccga
     5821 atctctcatc acttcatatt caatttcttt aaaacctgca atacttcgtt caatcaaaca
     5881 ttgggtagcg ggggaaagac ttaatccatt ttctgatacg atacgtaatt cttcttcatt
     5941 ttcacacata cctccaccag ttccgcctaa agtaaaggct ggacgaacga tgacaggata
     6001 gccgatttta tttgcaaaat ctacagcttc ttctactgta ttgacgattt cagactcggg
     6061 gattggctga ttcaattctt tcatcaactg tttgaataga tcacgatctt ctgcttgatc
     6121 gatcgcagaa agctttgttc ctagaagttc tatgttcaat tcttctaaaa tacctgatgc
     6181 tgccagctcc attgccatat tgaggcctgt ctgtccgcct aaagttggaa ggagagcatc
     6241 tggacgctct tttcgaagaa tccttgatac aaattcaagt gtgatcggtt cgatatatac
     6301 ttgatctgcc acttctttat ccgtcatgat cgtcgcagga ttagaattta ctaaaaccac
     6361 ttcatatcct tcttctttta aggcaaggca ggcttgtgtt cctgcgtagt caaattctgc
     6421 ggcttgacca atgatgatcg gaccagatcc aatcaccata attttcttga tatctgtacg
     6481 tttcggcatc tgtttactgc tcctttcctg cgtccatcat ttccataaat tcatcaaata
     6541 aatgtagtgc atcatgcgga ccaggggcag catctggatg aaactgcaca ctgaaagcgg
     6601 gataatctct gtgtttgact ccttctactg ttccgtcatt tacttctaca tgagttacga
     6661 tgagtttttc tggatcaatc gtggcttcat cgactgcata gccatgattt tgagaagtaa
     6721 aatcaattct tccggtcgct acttctcgta ccgggtgatt taatcctcga tgtccgaatt
     6781 tcattttata ggtatctgca ccgttggcta aagcaaataa ctggtgaccg agacaaatac
     6841 caaaaatcgg tacttttcct tggatttttt tgatcatttc gattgcttct ggcacatctt
     6901 ttggatcacc aggtccattc gtcagcatga ctccgtctgg tgatagcgct aaaatcgtct
     6961 cggcatctgt gttataggga agtactgtga ggttacagtt tcttttactc aattctcgta
     7021 aaatactatg tttcaagccg aagtcaatga caatgacttt ccgacctgtt cctggacttg
     7081 gataaggttt gttagttgag acttgctgca cttgattggt tgctagaacg gttgctttca
     7141 gctgatcaaa tgcatgttcg aaggaatctc cagcatcaac tatactagct ttcatcgttc
     7201 cgacttcacg gattttgcga gtaagggcgc gcgtatcgat acctgatatt ccaggaattc
     7261 ctttttgttt caaaaatgaa tctagcgtca tttggtttcg ccagtttgaa gctacacgtg
     7321 catgttcttt tactactact cctttgcatg ttggagaaat cgattcataa tcatctctat
     7381 ttaccccata attacccacc ataggatagg taaatgtaat gatttgaccg ttaaaacttt
     7441 gatcagtgat agtttcttga taaccagtca tacttgttgt aaaaacgatt tctccgaaga
     7501 cactagcatc tgcaccaaaa gcctttcctt cgaaaactgt tccatcttct aatataagca
     7561 aacgttccat acttttattc tcccttctgc cacacaagtt ttccgttgac aaaagtcatt
     7621 tgtgtttcgc cttttacttt ccagccgata aaaggtgtat tctccccttt tgataaaaag
     7681 tcttctttat cgatcgtgca agtttgtgta atatcaaaaa ctgctacatc agcgggggca
     7741 ccaaccgtta gcgttcctgc attcaaacca aaaatctcag ccggtttaac tgccaaccaa
     7801 ttgatcactt gttccaacgt gaatcgacca gtttctacaa aatgagtata gatcagttga
     7861 aacgctgttt cgcttcctac gataccaaat ggtgatttca tgaaactttg gcttttttct
     7921 tctaatccat ggggggcatg gtctgtagcg atgcaatcga tcgtaccgtc aagcaagcct
     7981 tcgatcaaag cttctctgtc ctcccgtcct cttagcggcg ggttcatttt ccaaaagccg
     8041 aaatcttcgg gaatatcttc gtcgatcaaa ataagatgat ggggtgaaac ttctgctgtt
     8101 acgtggattc ctgctttttt tgcgtctctg attacacgga cactttcttt agtagaaaca
     8161 tggcaaacat ggtaatgaac accagttgct tcagctagca acagatctct agcaatttga
     8221 gaagactcgg ttacgcttaa gatcccggga agaccaagtt cttctgcctt cttgccagcg
     8281 tgcataacgc cgccaaataa taaagattca tcttctgtat gggctactag ggctttattg
     8341 ttttttgcag cttctttcat agccagatac atcgtcccag ctgtttggac acctacaccg
     8401 tcatttgtga aagcaaaagc accttcttca atcagtgctt cttgatcaac taatttctca
     8461 cttcgcagat tttctgtgat tggcgcatac tgcaatacct tcacaactgc atcttttcta
     8521 atgatgtcat atacttgtct cagcttttct gccgtatcag gtacaggatt taaattcggc
     8581 atcgcgcata cagtagtgaa accgccacga gcagctgagc gagtcccagc ttcgatcgtc
     8641 tccttgtatg taaatcctgg ctctctcaaa tgaacatgga cgtccactaa accaggtgtg
     8701 atcaattgtc cctttgcatc aaacacttca tcaaattcgg cttctgagaa tcctgtaccg
     8761 attgctttga ttcgcccatc ttctatccaa atttctgctg gtgtcgtcat attttttcgt
     8821 gtattgatct gaccgttttt aatgagtgtt ttcatttttt catcctccta taattacgct
     8881 tttccattta gcactgcttc taagattgcc attctgacga atactccatt gctcatttgc
     8941 tgaacgatcc gcgactgtaa tccttccact aatgaatcag ctaattctac atcacggtta
     9001 actggtgctg ggtgcataat gattgcatgt ttttgcaact tcttcgcacg ctcaactgtt
     9061 aacccatgtt cctgatggta gccttctttt gagaaacttt cttttccatc atggcgttcg
     9121 tgttgcaccc gaagcatcat catgacatca acttgatcta gtagttcatc caatggcata
     9181 taatgtccgt acacttcgaa ttcatcgtcg taccattctc taggacctga gaagaatact
     9241 tgtgcgccta gacgttttaa catctgcata ttggatttag ctacgcgaga gtgagtgata
     9301 tcacctacga tggcaacttt cagaccttca aaatgaccaa attcttcata aatggtcatc
     9361 aaatcaagca agcattgggt tggatgctgt ccgcttccgt ctcctccatt gataatcgaa
     9421 cactggattg ttttactttg tatcagttcg tcataatagt tctcatcacc atgacggata
     9481 accgctacgt ctacgcctaa tgctgacatc gtcagtactg tatcgtatag tgtttcccct
     9541 ttttggacag agcttgtgct tgcttcaaat tcaatcactt ctagccctag ttttttctcc
     9601 gctacatcga agcttttatg tgtgcgagta ctattttcga aaaagaggtt tgtagcaaag
     9661 tactggcgct tttgcggagt ccaattagca ccttgcttga attcctgccc tctgcggatc
     9721 aagcccataa cttcttgatc agtaagtgcc tcaacggtca ataaatgctt taagctgata
     9781 cgttctgatt tgatgatcat aatgtttcct ccttagtttt cttcgcttcg tgaagtttct
     9841 ggtaagataa gatttaaaat gatgccaagt acagttgcta atgccattgc cgacaatgta
     9901 aatgtgccta cttcaaagac gagtccgcca attcctacta ctaagataac tgaagcaatc
     9961 aacagatttt tctttttatc aaaatcgatt ttgttctcga ttaaaatctt cattccactt
    10021 gcggcaatta ctccaaataa gataaaactg attcctgaga tgactggtcc agggatactt
    10081 aaaatcaaag cgcttaattt tcctacgaat cctaaaatga ctgcaaatac tgcagctcca
    10141 ccgatcacaa acacgctatg gacacgtgtg atagccagaa caccaatgtt ttcaccgtaa
    10201 cttgttacag gaggacctcc gacaaaggct gccacgattt gtgctaatcc atcacctgtc
    10261 aacgtcttat gaagacccgg ttcttcaaag aaattacgtt tagttagttt attcaatacc
    10321 ataagatggc cgatatgctc tgtcatcgtc acaaaagcga tgggtgccat tgttgtgatt
    10381 gctccaagat atagctttgg ttcatattgt acaaagggaa tctcaaagtt aggaacagca
    10441 aaccaggcag cgtctacgat tggcttagta tccacgattc caaacagcaa ggctacgatg
    10501 tatccgctaa taatacctag taaaatcgga atcaatccga gaaaacctct tagccacatg
    10561 ttgaagaaaa ttgttaatcc taatgtgatc agcgctacag caatatactt gaaatcatac
    10621 gtgccctgat tatacatagc atttgttgct gcatttgccg caagtcccaa cccgataacc
    10681 atgatgactg gaccgacgac gattggaggc aagattttat ccagccatgc tgaaccgatc
    10741 tttttgatga tcagcgcaac aatcaaatag actaatccag ttgtgactgc tccttgtgca
    10801 atcgctggat aaccatctgt tttcatcaaa agctgcatcg ctgcaatgaa agcgaagctg
    10861 cttcctaagt acgcaggtat ctttccttta gtagtaaata agtacaccaa tgtacctagt
    10921 cctgaactga ctagcgcaat acctggatcg atacctacta agatggggac taacacggtt
    10981 gctccgaaca tcgtgaacaa gtgttgtaaa cttaatccta cccaatgtcc tggggttggg
    11041 cgatcgtgga tatctaacac cacgtcatca ttatgaaatc cttttttctc cgacatgttt
    11101 ctctctcctc taatcttcct ctttcaaaat catgatgcga tcttgtccat caagctcttg
    11161 catctctact aaaatctctt ccgcccgaga tgttggaatg ttcttcccaa cataatctgc
    11221 acggattggc aattctcgat gcccacgatc gaccaggact gctaatgaaa tttttcttgg
    11281 tctgccaaaa tccattactg cgtccattgc tgctcgaatc gttcgtcctg tatacaaaac
    11341 atcatcaatc aaaataactt cttttccttc taaggaaact gggatgtcag aagaatgaag
    11401 ttccggttct tctggatttt cttttttgtc atctcggtat aaagtgatat ccagttctcc
    11461 tacaggaata tcgatatctt ctagctgctt caggcgttcg gcaattcttg atgcaatgta
    11521 gattcctcgt gttttgatac ctaccaatac gatatcttgg atactatggt ttctctcgat
    11581 aatctcataa gtaattcgag tcaatgctcg cttcatggtt acctgatcta ctacttcttt
    11641 tgcttgcatt ttcattcccc ctactttttt gcataaaaaa actcctgccc taagaggaca
    11701 ggaggaatgt ttagactgag cgtatactga tacactgata gctatactat gtcaatgagc
    11761 aatctactta tggcgcacca tacgtatcct tgctcccacc cttcttagcc tcacgggact
    11821 aaacttaaag gaattctatt aagttttatg ctttcgttgt taattactat accgttatct
    11881 aaattaaaat gcaatgtttt ttcttaattt ttctaaagtt ttttcaaaaa cttctggaag
    11941 aggtgcttca aagatcatct tttcgtttgt agtcgggtgc gtaaaaccta acatttgcgc
    12001 atggagaaac tgaccattgc ctttcaaagt tttcttcggt ccgtatactg gatctccagc
    12061 taacggataa ccgatatact tcatatgcac acggatctgg tgtgttcttc cggtttctag
    12121 ttttaattca accagagtaa atccttcgaa acgttccaat acagtaaaat gtgtgacagc
    12181 cgacttccca tcttctctta tcgcctgcat tttccggttt tccttcgaac ggccgattgg
    12241 cgcattgatg gttccttttt cgtgaggaat ctctccatgg actaaagcaa catatttgcg
    12301 caaagaggtc ttatctttta attgctgagc taatgattcg tgtgcctggt catttttggc
    12361 aaccattagc agtccggaag tatctttgtc gattcggtga acaatacctg gacgaataac
    12421 gccgttgatc gtagaaagat ccttgatatg gtacatcagc gcattgacca ttgtgccatg
    12481 cgtatgacct gctgaaggat gaacgaccat tccttgaggt ttgtttatca ccgctacatc
    12541 ttcatcttcg taaacgatcg tcaacgggat attttctggg accacatcta atgtttctgg
    12601 ttcggggatc aagacagtaa tctcgtctcc ttgttttact ttgtaatttg ctttgactgg
    12661 ctgtccattg acagaaaccg ctccattttt cagccacagc tgaatttgcg aacgactgta
    12721 atcggtgaat aaattagtca atactttatc gatcctacca gattcatggt gaatcgtcgt
    12781 tttaagttct gtcatttttt tctttgatcc ttttcatcta aaataatata aattaacata
    12841 caaatcactc cacatacaag tgtcacatct gctacgttaa aaatcggaaa gctcataaaa
    12901 tctgtctgga acatatctac tacatagcca agacgaatac ggtcgataaa attgccaatc
    12961 gcaccagcta gaatcaaact caatccaatc atgaaccagc gattaccttt ttcacgattt
    13021 ttaatcatca atgtgagaat cacaacgact gcaatgagtg tcactatagt aaagaaccac
    13081 atcttccctt ctagcatact ccatgcggct ccagtgtttc gaatatgatt cagtgacaaa
    13141 atacctggta taagattttt cgtctctcca agcgctagat tatcagtgat ccaccatttg
    13201 accaactgat ccactcctac tagcaaagcg ctgaatatcc aataaataat taacaaaaaa
    13261 tcaatccttt tctatttgtt tataaatatc acgaggtcgt acgatcccga gcagattacc
    13321 atcgggattc ccatctttag tgataagtac ggcttccaaa gtcccttgct cacgaaacat
    13381 cgcttcaacc tcgtaaatat gtgttttagc tggaacaaaa tgataatttg tgtactttcc
    13441 gttttggatg atcaaatcca acgctcgttt atttgttaaa tcaaccggac ctttttggct
    13501 ttcttttgct agccaaaacc cgatattacg taaagtaatc aatgctacaa attttccttt
    13561 attgtataac ggaaattgtg aatacctttt ttctgctact atctttaaca gttcaagtaa
    13621 cggaagatct tgttcaaaac ctgttacacg ttttgcaaat cgaggcagca cggtttcagg
    13681 ctgaatcagt tcttgttcga tcagctggat tcgcttgact gtccaatcat ttggctctgc
    13741 aatgacaaaa tcgactgcga tccgatcatg gacgatcgca ttccttaact gagcaagctg
    13801 taaaagatca tcttcgtatt ttctgatcat taattgtttt ctttgagcta ggcgtctgac
    13861 aagttcggta aatcccatat ttcgtgcatt tcccatctcg tctctcatcc atttttcgat
    13921 tcgattgaaa ctgattaaaa aaagatcaga gtttgccata attttcctcc ttgatcaaat
    13981 atgcccggtt ttttatatag aaaaagaacg caatcaacac aataactgca aaaaaactga
    14041 aaaataaatt ttgtaggaaa acggccccca acccagttgc tagaaataaa aacggataga
    14101 tttgaagaat caagtaattc attgtgatca cataagcagg ctttgcttct ttcactgtat
    14161 aaacggggct ttttagccaa taaaaggtga cctgcatcgt aaagggcact tccagatcaa
    14221 gcgttatctt ttgttcatga ctgagaacac tgcgtttttc attgttgatt cttagctgaa
    14281 tcgagctacc cataccgtaa aaaccggttt ttcttataaa tgtcgttttc atcataccct
    14341 cctattatgc tttagtatag ctcaaaatac aataaagaaa aagaatgatc ctagtgtaaa
    14401 caaaattaat tccctatcgt ctaaaaaaag aagctgtagt caaaatacag ctccttcgat
    14461 ggtagattat aatcaagctt aaaacaatcc caacacttct ccgtcatttg tcacgtccat
    14521 attcaatgca gcaggttttt taggcaaacc aggcatcgtc ataacatctc cagtcaaggc
    14581 tactaaaaat cctgccccta attttgggat gatttcgcgg atcgtgatgg tgaaatcttc
    14641 cggtgcacct agcagtgaag gatcatcaga aaatgaatac tgtgtttttg ccatacaaat
    14701 cggcagtttg tcccatccat tcttcgtaaa ctcttctatt tgtttttgcg cttttttgct
    14761 gaaaacaaca ccatttcctc cgtaaatttt cttgacgatt gtttctgtct tttcttgtat
    14821 cgtttcttct aggcggtata atggcttgta attagcttct tttctatcaa tcaatcgtac
    14881 tacagctttt gctaaatcaa cgccgccttc tgctccatct gcccatacac tcgctctctt
    14941 ggcaagaatc ccctgatttt cacacaagtg ctccaacagt gtgagttcat tgtctgtatc
    15001 tgttacgaat tcattgattg cgacgatcac aggtagctga tattgttcca tattacgtat
    15061 atggcgcttt aaatttgcaa aacctacttt caaagcatca agattctcat ttttaagttc
    15121 atcttttttc attccgccat gcattttcaa tgcacggaca gtggcgacaa tcaccacagc
    15181 atccggcgct tttttcaagt taggtacttt gatatctaaa aatttctctc cgccaagatc
    15241 tgcaccaaat cctgcctctg tgacaacgta atctgctaat ctcaatgctg tcttggtagc
    15301 taaaatacta ttgcagccat gagcgatatt ggcaaacggt cccccgtgga caaaagctgg
    15361 tgttccgtaa attgtttgta caagattcgg cttgattgca tctttcagca gtaatgccag
    15421 agcgccttct gctttcaagt ctccagcagt cacaggctga cggtcaaatg tatagccgac
    15481 aacgatcctt gcaagacgag cctttaaatc ttcaaggtct gccgcaagac acaagatagc
    15541 catgatttcg ctggcaactg tgatatcaaa cccatcttct cttggaacac cttggattgg
    15601 cccacccaag ccaacaatta ctttacgcag ttcacgatca ttgagatcga ctacccgttt
    15661 ccaaatcact cggcgggcat cgatgttcaa ttcatttcct tgatgaatat gattatctaa
    15721 aagagcagat aatgcattat tagctgttgt gatagcatgc atgtcaccag taaaatgcaa
    15781 gttgatatct tccataggca atacctgtgc atatcctccg cctgcagcac cacctttgat
    15841 tcccatcaca ggaccaagtg agggttctcg caatgcaata acagattttt tattgatttt
    15901 attgagcgcg tctcctaacc ctacagtgat cgttgacttt ccttcgccag ctggtgttgg
    15961 attgatggaa gtaactaaga tcaaatgccc ctcagcgtta tcctttgatc gattgattcc
    16021 agaaaaattg attttcgcct tatatttccc atacagttcc aaatcgtctt ccttaagatc
    16081 taatttacta gccacttcaa caatcggttc tagctctgtt tcttgtgaaa tttgcagatc
    16141 attcttcatt tgattacccc ttttcacgaa ctttattcaa caaaatatct tctttgttaa
    16201 gatatgccaa ttataccttt ttttattcag aaaaaaaaca taaaattaca ggtttttaat
    16261 ttatttgacg tacttataaa tactctttat aatgaaggct atgatcagac tttagaaagg
    16321 tcgtgagtgc tttgaccaca ggaattgtaa aatggtttga caacaaaaaa ggatacggct
    16381 ttatcagtta tgatgacaca gaagaaattt ttgtacattt tactgcgatt gaagaagagg
    16441 gattcaaaac tttggaagaa aatcaagtgg tcgaatttga gatcatcgaa gggaatcgag
    16501 gaacacaagc tgcccatgtc aagaaaggca acgttcagac taccgatact agtatgcatg
    16561 aatagataaa caaatagagt gagacgctaa acgggatgaa ccgtacgtct cactcttttc
    16621 ttttcctgta aaaattcgtt tttttaggtt ctgattttta aaattagctt tttctttttt
    16681 tcgtataagg ataaggaatt gattctgatt tgggtccgtt tttttcaaaa tgatcaaaaa
    16741 ggactacttg atcttttttt cgcatcatga aaacagatgg ggctttctca tcttgcaaca
    16801 tcatttgcac cccagtatta gttggcgtca aagatcgaat ctgtccgaca gatatttctc
    16861 ggtctttcca aaaagcagca taacgtataa tcacagagtt tgattttaaa ataaaagaac
    16921 gccgcaaacc tagaaataga aaaacaagaa aaatacctaa aacaaaattg cttttccaat
    16981 aaggacctgt gttttctaaa gaaagaatca gactaaaaaa cagaatgatc aaggtcaatg
    17041 accaatagat gatcgtatgt gccagttcag gctgccatcg aatatttttt ttcattctct
    17101 aacctctcac cgattcagta atgatataat cttaccataa cattagtttg aacaaaaagg
    17161 agaatctctt tttatgttaa aagtatacat tgatgcctct acaaaaggca accctggtcc
    17221 tagtggaggt ggaatcttga ttgtctatca aggtaaacaa gaacagctat ctatcccgct
    17281 ttctataggg acaaaccatc aagcagagtt cgaagtcttt ttaaaaactt tagaatttct
    17341 aaagaaaaac gacttacaga atgaaacgat tttctgtttt tcagacagca aaacactagt
    17401 ttctacggtc gacaaaaacg caacgaagaa cgagctcttc cttccctacc ttcaaagaat
    17461 ccaagaactt ctatccgaat tttctttatt gatcctgcaa tggttgcccg aaaacaaaaa
    17521 taaaggagca gataatcttg cacgtcaggc tctccataaa caattaaaag aaaaaaagac
    17581 tctttaaatt aaaaacttta aggagtcttt ttatagtttc tcatttaata taaaagataa
    17641 ttgtgatcaa acgtaatttc caattggtaa tctccgattt tttcttctat ttgactacgt
    17701 aagattcttt ctagttctgt atcaacaaac tggaatttct cagggaccac tacatcaaat
    17761 tgcagcgttt ttccttgatg aattactcta aaatcatgaa tattaagccc tgtacctgtt
    17821 tcaagaagaa tttgctttaa ctgccgcaag atattatagt atttttcgtt atcaacaggc
    17881 attggatcca agtgacagac aagttccaca tccaaagact ttttaaagtc tttttctatc
    17941 agatcaataa tcttatgagc cacattcaaa ttcattgatt catctacttc gatgtggact
    18001 gatgcaaagc gtttattggg tccatagtta tgcaccaata aatcatgata gccaagtata
    18061 tcatcgcaag catttaaatg ctcttccatt tcttcaatct ctgctggcgt tgggcgactt
    18121 cctaatagtt cgtatacaaa atttcgaagc atcgtcaatc cactataaag gatatataat
    18181 gctaaagcaa aacctatata tccatcaata cgccatccag ttacccattc gattgctgct
    18241 gaaagcaata cagccagtgt agtaaagaca tcattataac tatctgttgc tgtagctctc
    18301 aatgtttcag aatcaatgga ttgcgcaata acctgataca ttttgccttg tgcaaatttc
    18361 aaaaagatgg aaatgattag gacaaaaaag acgatgggtg ttaaagagac attttctgga
    18421 tggatgattt tttcagcaga agactctaaa aattgaaatc ctacataagt aatgatgatc
    18481 gaaacaaata acccactgat atattcgaag cgttcgtgtc cataaggatg ttcctgatct
    18541 gctggctttg cagcaatttt aaatccgatc aaagtcaata tggatgaagc agtatctgat
    18601 agactgttca ctgcatcagc cataatggag acacttcctg aaaaaagccc gatcattagc
    18661 ttagagacaa acaagatcag attcgtaacc aatcctaaga tacccgctag tactcctttt
    18721 tttgtccgct gttgcggtat ttgtttcttt ttttcttctt gtttcttagc caagtttttt
    18781 accccttcta gtttgcttgt tgctcaggta aaaagatgga gaggacgatt gttttcctct
    18841 cctctcttcc tttttctatc tatcgtgcgt cctattacta ttaaaagatg gtcattattc
    18901 tggtttcaac ggtgttgctg caaataacgc ttttgctagt aatggtgtta tgattcccgc
    18961 acctattgct tctggtatcc cattgattgc tgcaacccct gctaaggttt taaataaaag
    19021 agcagagtct acaccatagg catctgccgt tgcacctgta taaaataatc ccatgaatcc
    19081 taacactaag actgtattga ccaatgaacc cagtacccct gcaaaggctg agctgatcgt
    19141 tattgattta tttttttgat aaatccagtg gaaaaccaag cctgctacta agccaacaag
    19201 tactctggga actaccgaga taatcggatt cgtgaacact aatgtatcaa atggcgttgt
    19261 tggcatggca taggcacgaa caattgtagc gattccccaa aacaagccaa tcaccatgcc
    19321 atcttttggg cctaatacta ctgcagcaat aattactgtg atatggataa ttgttaaact
    19381 aatgaatcct aatggaataa accccagcca aggaaccatc gcttgtacga tgatgattgc
    19441 taacaagatc gcacgtaaga ctagtcgaaa ggtcttattt ctactattca ttttttcacc
    19501 cctttttatt tatactaatc gtaaaaattt ctgtcacttt cgtcaactaa agaccatccg
    19561 aatccgacca tttgattaga tgcgccatcc aattgaaaaa agtatgaggg caagacttgt
    19621 ttgtcttcgc ccccataatc atgtttcgtt caatgtttga acttttgttt ttctgtcaaa
    19681 cgtaaacgcc ctattgttgc gcaacccatt ctttcgcagc tttaagtgca tctggaatac
    19741 cagcaggatt tttcccgcca gcttgtgcca tgtctggacg tccaccaccg ccaccgccta
    19801 ctttcggtgc gattgcttta atcaagtcgc cagcttttag tccagcttca tttgattctt
    19861 tcgtcattgc cgctagcaga ctgacttttc cttcttgttc gtttgctaaa acaagaatat
    19921 ctgaaatatt tttttgtttc cattggtcag ctaattgacg cagctgattc atatctttca
    19981 cttttacttg tgctgcaatg attgtttttc cattgatcgt ttctggatct ttgaatacat
    20041 caccagcttg ttgattcgct aacttgctag ctaattgttc attttccttt tgtagctctc
    20101 ttacttgctg ttgcaattgt tccgcttttg aaacaacttc tttcaattga ggtgatttaa
    20161 caattgttgc tacttgtttt agttgttttt cttcttcttc aagtagttcg tacgcttctt
    20221 tacttgttac agcctcgatt cgtcgcacgc ctgcaccaat tcctgattca gaaacgattt
    20281 tgaagattcc gatgttttct gtatttgcta catgtgtgcc tccgcacagt tcgattgacc
    20341 agccgccaat atttactact cgaacgtcat gtccgtattt ttcaccaaac aaagccattg
    20401 cacccatgtt tttggctgta tcgatatctg tttcaattgt ttcaactggg atagcttccc
    20461 agattttttc gttgacgatt ctttccattt cagctaattc atctgaagtg atttgaccaa
    20521 agtgggtaaa gtcgaatcgt aaatgtccag gagcgactaa agaaccagct tgatttgcat
    20581 gctcgccaag tacttctttc aatgccttgt gcagtagatg ggttgctgta tggtttttga
    20641 tgatccgatt gcgcatcgat tgatcgatca atagatgata ggtttgtcct tcactcattg
    20701 gtgcttttac ttctacggtg tgcataaatt gaccattagg cgctttttga acatctgtaa
    20761 caactgctac gatttcgcct acttcattca tgatccatcc ttgatccgct acttgcccac
    20821 ccatttctgc atagaaaggt gtttgatcaa agatcatttg agcttcacct tctgcgactt
    20881 cattcaaaag agactcacct ttaacgatga ccaataattg actatctgat tcttgttctg
    20941 tgtatccaat gaatttactt tctactttga tatctgtcaa taaagcagat tgtaccccca
    21001 ttgatttttc agtactgcgg gcagagcgag cacgttcgcg ttgtgcatcc atttcttttt
    21061 caaaaccagc atgatcaact ttcagtcctt catcttctgc cacctcttcc gttaattcta
    21121 ccgggaagcc atatgtgtcg tatagtttga agatgtcttt tcctgctaga gtcgtcccgt
    21181 tttcgttttt tacttcttgg atcacttgat ttaagatttc caaaccttca ttgattgttt
    21241 cattgaaacg ttcttcttct gtacggataa ctttttcaat aaattctttt tgttgaagta
    21301 cttcaggata ataacttacc atgatttccc caacaacagg gactaaacga tataagaatg
    21361 cttgttcaat acctaatttt ttcccatgca tgacagcacg acgaagcaaa cggcgtaaga
    21421 cataccctct cccttcattc gatggcaatg caccgtcgcc aatagcaaat gaaagggcgc
    21481 gaatatgatc tgcgattact ttgtaagaaa tatctgttac tgggttttca ccatatttta
    21541 cttgtttgct tagcttttct acggcgtgga taataggcat aaacaagtct gtctcaaagt
    21601 tcgttgacgc atcttgaacg attgagacca tgcgttccaa tcccatcccc gtatcaatgt
    21661 ttttatgagg aagtggttca aatgtgttat caggcttatg gttgaattct gaaaatacta
    21721 agttccagat ttctaaataa cgttcatttt ctcctcctgg atagttttca ggatcgtctt
    21781 ctgctaaatc gttataagct tctccacggt catagaaaat ttccgtatcc ggaccacatg
    21841 ggcctgcacc gatatcccag aagttgtctt ctacatcaat aatgtgatct agtggcaggc
    21901 caacttcttc atgccagatg cgttttgctt ctgtatcttt tggataaact gttacataaa
    21961 gtttttctgg atcgaatgcc atccattttg gatcagtcaa aaattcccat gcccagtgga
    22021 tggcttcatt tttgaaatag tcaccaatcg aaaaattacc cagcatctca aacatcgtat
    22081 ggtgccgtgc tgttttaccg acattttcga tatcatttgt tcgaatagat ttttgggcat
    22141 ttgtgattct tggattttct ggcacaactg aaccatcaaa atattttttc aatgttgcaa
    22201 caccagagtt gatccacaat aaggttggat cgttcacggg gatcaaagac gcactaggtt
    22261 caatcgtatg tcctttagat tggaagaaat caagaaacat ttgtcgaact tgtgcactac
    22321 ttaattgttt catgtcatta cctgctttct ataaattaaa tacaaaaaaa caccatcaca
    22381 gccaaggacg aaaactcgcg gtaccacctt gattgcaatg atgtgtaatc attacctctt
    22441 aaactcgata acgctgagct gcgtcggtat ttctaccaaa atagaaaaag ggagcaattt
    22501 gattagtgac tattcttttt tcagccaaga aagaattttc tgtgagtctt aaaaaacaaa
    22561 tcatatcctt ttcgtactta attagtatac gcattctatt gatcactgtc aatatctcaa
    22621 tttttatttt catttccttt tggtcatact ccacgatttt tggtacgata agaataaagg
    22681 aatcaataca tctctgctat tcatgacaag gaggaatatg atgaaaaaaa tagttggagc
    22741 ggatgaatgg atacgcgcag ctagctgctt tttgagatat caagtttttg tattagaaca
    22801 agggattcct gcagaactag aattcgacgg aaaagatatc ccgcaacaaa cctattatct
    22861 atttcttgat caaaaaagac cagtagctac tttgcgttat gaaaagaaaa cacctacttg
    22921 cttcaagccg gaacgatttt gcgtagcaaa agaataccga ggaaaagggt taggcggtca
    22981 gttgcttgaa gctgctgaaa aacgggcggt ttcagaaggt tgtcagacta gttatgtagt
    23041 agctgaaatg acggctgtag atttttatga aaaacaaggg tatcatactt gcacagaacc
    23101 ttttattgaa gatgaagtac tttgtatcgg aatgaaaaaa agtctagctt aaaaacaaga
    23161 gattgtgaca cgccttttgt cacaacctct tgttttttat ttatgtgaat ttttcttatt
    23221 tttacgagca gtacggcttt gctgtctgcg ttcgattttg cgttcttttt tatttttttc
    23281 tgcaattgcc caatcgattt ttttcttata accaggcttg atctttttct ttttcttctt
    23341 cacaagacca atcagcgtgg gatctaatac atcttttgat ttttctcgtt tggcacgtct
    23401 attacgatcg tatgttttga cgatttcccc gtttttgatt tcttttggtt caaatgagac
    23461 gcccaatttt tctatcgcat caatggcttg ctcatcattt ggcgaatata acgtaatggc
    23521 cgttcctttt aagccattac gtccagtacg tccgacacga tggataaaga agtctagatc
    23581 ttctggaatt tctgcattaa taacatgaga gacaccttcg atatcgatcc ctctagcagc
    23641 taaatcagtc gctacaacat attgatactc cagattctgt acttgtcgca tcactcgttt
    23701 tctctctctt ggggtgatgt ctccatgaat ctttgctact ttcaagcctt ggtttttcaa
    23761 gtaatcggca atttcatcca catgctgttt tgtattggca aatacgattg ctaaataagg
    23821 atgaccaatt gtcagcagtt gataaatcat ttgattctca tctttacctt ttgttgaaat
    23881 cagccaattt tcaatgtctt cagaaataac tgcttttggt ttgatttctt caatgattgg
    23941 attttccatg tattttcgta agaatggacg aagtttttct ggaatagtgg cagaaaaaac
    24001 taaaaactgc agcttttctg gtaatcgact tgcaatttgg tctacttcat ttaaaaaacc
    24061 catatccaat gtcatatccg cttcatcgac aacaaatgca aatgcggtgt gaatcttcag
    24121 cgcttgttca ttcatcatgt ctaatattct accaggcgtg cctactacaa cctgtggctg
    24181 ttggtgatgc agacgattta attgacgctg tttgtctgta ccgccgacaa aattcgagac
    24241 acggatctcc ggtgtagaaa atttagcgat ttgtgtagct gcttcataga tctgtgcagc
    24301 tagttcccta cttggagctg taataacaac ttgtacttcc tctttttctg cattgatttt
    24361 ttcaaacaaa ggcaacaaaa acgtatgtgt ttttcctgta ccagtttgag attgcccaat
    24421 gatatttttt ccttttgcaa ttaaaggaat caaacgttcc tggacctctg tgggctgttt
    24481 gaagcccttt gcggttaacg cttcattgat aaacggtttg aagcggaata tatcaaaatt
    24541 attcattcgt gcacctcttc tctttaaatt atcacgactt tggcattata acacagacaa
    24601 agcgtttttg ctcagctaat ttcgagaatc atcatccttt gtttggccaa tcgtgctaac
    24661 agtatatgtg tgactagctt tttcataaaa cagaatccgt ttttttctca aaaatgatag
    24721 caagatattt aaaataaaaa taccttgtag taacaagaca ccgagagaaa tgaatactgc
    24781 actcatctgc aacaccgtat tcgttgtcga attcatgatt tgctgaagca actgccccaa
    24841 ccagcttaaa ccataaaaaa gaaagtacag atacccgtag cggatcatca atgcttttaa
    24901 agaaattcgt tcgcggtctt cttccaccaa acggatacgc acaatttttt tgccaagtgt
    24961 ccatccattg gttataaacg gtacaatcat aaagtatacc cctacttcta ataaaagcca
    25021 ccagatatga gaagtaactg cccctgaatc gaacgcaatt ttaaagaaaa tcgccgctac
    25081 caaattaata agagataaaa atgtccagtc gatcaaataa gcgaccaagc gtcgagtcaa
    25141 tgagacagtc tttccttttt cataagaaag ttcatccatt cgtcttcttg taggaagcat
    25201 aaatgtaaat aacggcgtaa cgacaaaacc aatgattcca cccaatgtat ttgtcactaa
    25261 atcattcaca tcaaataagc ggtatggtct tgggtagata aaataaagtc cacttagctg
    25321 cgtcaattca aaaaatagag aaaggcagaa actagcaatc atcgttttca taaaagaaag
    25381 ccggaaataa tatcttaaat aaatgccaaa cggtatgagt aataaaacat taaaagctgg
    25441 ttccaaaaat acactctgct tcattgccgg caggtaggta ctaggttcag acaagaccaa
    25501 taccgtgttg tctctgaaat gttgccatga agcaaataaa tgcagctcca tctttggccc
    25561 tgtcatttgg gcaaccgtct cctttgccgg aagtggtaag atcaccaaaa aatacgcaca
    25621 caataaataa aaaacgaaag aatacaaaat aactgccctt gttactagaa atgttccata
    25681 ctttcgatat tcataaataa aaaaaacaga tgagatcgct aaggctaaaa atggaaaaac
    25741 aatcagcgca aaacgaatcg gttcgatata acttgccata tgactcctcc ttgattcttt
    25801 gtatctctct tatcttagca gtcctttgct gtttcaagca agtaacgagg ctattatttc
    25861 tcatttcttt caaagtaaag taagataaac acaaaggggg tgtcgagatg ctgaatatgt
    25921 actttgtttt tggcgtacct atttttttac tgttccttta tgctacgatc gcttatgtaa
    25981 gaaaaagaac tacaattcat tatttaggat ttattctcct gattatttcg ggtttcatgc
    26041 tagtcttcaa tcttcagact tggcagcaag cacttcttga aatggataaa atgactccac
    26101 atgctttgtc taaagtgctt ggctaccctg tctatctgat ttggcttccc attttcattt
    26161 ctggttgctt ggttttatta aatatttatc gcggcgtacg ccgaatagtt cagttacgca
    26221 aatcaaagta aaagacagaa atcggttgag cccgatttct gtcttttact ttgctatttc
    26281 atcgtagaca aaacgacatt tacgtgatca aaattaacga gtacttttga catttcttcc
    26341 acgtactcct catttaaata aaatattttg tttggcagag aagtgcagtt ctaataaaat
    26401 tcgaatgttt tagctatcta cgttttaaat agagataaat cgggatacca gcaagtgtca
    26461 atactatccc catcgaagct aaaaaggtct gagtgataag cgtgcttgat acgataaaag
    26521 tccctccaat gattgctact agtggaataa acggatacat tggaactttg tatggtcgaa
    26581 acaaatcagg ttctttttta cgaagaagaa tgacaccaac aaagaccatc gtataaaaaa
    26641 tccaaatgac aaagatcagc atatccgtta gtgtatcaaa accaccgatc atcatcatgc
    26701 caatagcgat gactagctct aagatacctg cggctacagg aactttagtt ttatcatgaa
    26761 gtttagcaaa gagcttgcta aaaggcaggt tgttttcttt tgccataaca taagggagac
    26821 gcattccagt aagggtatag ccattgatgg ttccataaac agaaatcaag ataccaactg
    26881 tgaccagtct tccaccgaag ccgccaaaaa tcatttttgc aacgtcactt gccgcattgc
    26941 tgtttcctgc gactccgtcg atagatgcgg ttcttaagaa caccgcattt actaataaat
    27001 aaacgatcat gattccgatg attcctaaag aaattgcttt tggcaaatct ttcgccggct
    27061 ttttcaattc tcccgaaata tttcctacgt gtatccaacc gtcgtatgcg aacatcgttg
    27121 ctagcaagcc cgctcccaag gctgagaaaa atgaaagatt ttctcctgct tggatcggaa
    27181 acagttggaa gtctactcct tcttgtcgga acaaaccgaa aatgacgatc acaaacaaag
    27241 gaataagctt gcagacaagc gtaatcgact gaaaagcccc tccagcttta gaacctaaaa
    27301 aattgatcaa caggatcgat acagcagctg taacagctac gggaacaatc attgactggg
    27361 aaagaccaaa tagattgacg aactgtgtac cgaaaatgat cgacaaggct gctacatttg
    27421 ctggaaaata aatcacgact tgcgcccagc ccaataaaaa agctgctgta tttccgtaaa
    27481 tccgttcgat atatttaatc atccctcctg tttctggaat agcagctgcc agttcagctc
    27541 ctgttagtcc tgcacagaca gaaatcatac caccgagaaa ccaagaaaac atatgcaaac
    27601 tggcggatcc tgtaacttct gctacgcttg cggctttaaa aaatacccct gcaccgatca
    27661 ctgtgcccat aacagtcgat aaagcagtaa aaaatcccat cgtacgtttt aattcatttt
    27721 ctatttttac ttcttttact ggcgtggctt gatattcttc catcccttgt cccctttcat
    27781 accatagcca atcccaaaag tataaaaaac tttttggatg tggttcttgt tttataccgt
    27841 ctctaccaat ttttctttca gtttttccaa catatccttt gtcatcacat ctaagtcgta
    27901 tttcggcgaa aagccccatt cctcttgcgc acaagaaaca tctagtgaat ttggccaaga
    27961 ttctgctatt gactgacgta caggatccac atcataatcc atttcaaaat ctggtatgat
    28021 tttttgaata gatgctttga tctcttccgg gtcaaaagac atcgccgata tattgaaggc
    28081 atttcgatga accaatcgat caggatctgc attcatgagt tttataatgg catcgatcgc
    28141 atctggcata tacatcatat ccattgatgt accttgatca atgaaactag tgtactttcc
    28201 ttctttgatt gctgaataat aaatatcgac tgcgtagtct gtcgtaccgc ctcctggcaa
    28261 ggttgcataa gaaatcaatc cagggaaacg gacaccacga gtatctacgc catatttcac
    28321 ataataataa tcacatagta gttctccagc taccttcgtt accccataca tggttgtagg
    28381 acgctgaaga gtatcttgtg gggtatgatc ttttggagta tttggaccaa acgcgccgat
    28441 tgaacttggc gtaaaaaatt tcagctggaa ttctctagcg acttccaatg cattgaccag
    28501 cccagtcacg ttcaaattcc aggctaattg tggttttgct tctgcaacag ctgacaaaag
    28561 agcagccaaa tgaatcaatg tgtccacttg atattgttct gctatgctgc gcattttttc
    28621 gtaatccatt acatctagga tctcaaagat atcgtttttc tcaatgactt caggcatgcg
    28681 tatatctgtc gcaatgacat tttcactgcc tagttcttga cgtagccgtg tgatcaactc
    28741 tgaaccaatt tgtcctaaac atcctgtcac caatactttt ttcatgatga tcctccctga
    28801 tcttttttac tactatcttc agatgattaa caatatatgc gtgaacatgt tctctaattt
    28861 taaatagacg aaaaaactaa atttagttaa ataatttcca tttcttttcc tattttttga
    28921 taaactgcta ctgcttcatc aagcatttcc ctcgtatgtt ccgcagtcgg catatttctg
    28981 atcctgcctg tcccaagagg cacagttgga taaacgattg gtttagcata aatcccatgt
    29041 tcgatcagct tcttagagaa agtctgagtc actttttcgt ctcccaaaat gactggagta
    29101 ataggtgttt cagacatacc gatgttatag cctacttttt tcaattcttc tttaaaataa
    29161 ttagcatttt cccaaacttt ttcgacaagt tccggatgtt cttgcatcaa agtaatcgaa
    29221 gccaatgcag ctgcagctgc acctggagtt agcgaagtag aaaataggaa tggccgactt
    29281 ctagctttca accagtcgat tagtgttttc gatccagcta cataaccccc aacaacacct
    29341 atagctttcg ataacgtgcc catttgcata tcgattttgt cgcttaatcc aaaatgcttg
    29401 acggttcctg caccttttcc agttacgcct gagccgtgcg catcatcgac gtaggtgatc
    29461 agtccatatt tttcaacaat cggaataatt tccggcaatc gtgcaatgtc tccatccatg
    29521 gagaaaacac catctgtgat atacatgatt tttttatatt tcttactttc tatcgcttcc
    29581 tttgcttttt tctcaaggtc tttcatatct tgatgtttaa ttcggatgat ttttgctccg
    29641 gataagcggc aaccgtcaat aatagatgca tggtttaatt catccgataa gatagcatct
    29701 tcttttgtca ttactgcaga gatagcgccc atattgcagt tgaatccaga ttgaaaggcg
    29761 attgctgctt ctgtcccttt gaattctgcg attttttctt ctaattgctg atggattttc
    29821 agcgaaccat tgatcgttcg gactgctcct gctcctacac cataagtttc ggttgcttca
    29881 atacatgctt ttttcaattc ttcgcgattg gcaaatccaa gataattgtt ggatgctaga
    29941 ttgatcatct tttttccgtc tacgatgata caagcaccat tttcactttc tagtatgtcg
    30001 atcgtattgt ataacccctt ttctttcagt tgatccaatt ctttctctaa atacattgtt
    30061 agttctttgg aagacattgt ctccacctcc atattcatct gtcttgcaga acataccaaa
    30121 catatatcgt gtcattcatc tacaaagcaa gtatgctata tttttgataa cgcttacact
    30181 gattatgaga attatttaac cacatttttt atttacagta tctttttact ttcaaatgac
    30241 ttatatagta tgaacaatta ccttttagat aatgatctat tttactctac ctgggcagat
    30301 aaatacaaca caaccattga tagaagccct ttcgttaaaa tcaattgtat tctgaatatt
    30361 taatttattt attgcataga ccaataaaaa tacataaaat ttccatcttc gtttatcagc
    30421 aatttttaat tttactctct gaatatttct taaaagtttt acttgttgaa ttcgaaaaaa
    30481 ggactgtgac aaaagtcttg tcacagtcct attctt
//
LOCUS       DAZKOR010000028        29994 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000028 DAZKOR010000000
VERSION     DAZKOR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.028"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..1103
                     /locus_tag="ACG8ZM_001158"
     CDS             78..1103
                     /locus_tag="ACG8ZM_001158"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0492131.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            1142..2092
                     /gene="manA"
                     /locus_tag="ACG8ZM_001159"
     CDS             1142..2092
                     /gene="manA"
                     /locus_tag="ACG8ZM_001159"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0492132.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            2186..3889
                     /locus_tag="ACG8ZM_001160"
     CDS             2186..3889
                     /locus_tag="ACG8ZM_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0492133.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            3891..5923
                     /locus_tag="ACG8ZM_001161"
                     /pseudo
     CDS             3891..5923
                     /locus_tag="ACG8ZM_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
     gene            5934..6374
                     /locus_tag="ACG8ZM_001162"
     CDS             5934..6374
                     /locus_tag="ACG8ZM_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0492134.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(6574..7701)
                     /locus_tag="ACG8ZM_001163"
     CDS             complement(6574..7701)
                     /locus_tag="ACG8ZM_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0492135.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(7704..8381)
                     /locus_tag="ACG8ZM_001164"
     CDS             complement(7704..8381)
                     /locus_tag="ACG8ZM_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0492136.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            8645..10411
                     /locus_tag="ACG8ZM_001165"
     CDS             8645..10411
                     /locus_tag="ACG8ZM_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0492137.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            10426..10863
                     /locus_tag="ACG8ZM_001166"
     CDS             10426..10863
                     /locus_tag="ACG8ZM_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0492138.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            10876..12027
                     /locus_tag="ACG8ZM_001167"
     CDS             10876..12027
                     /locus_tag="ACG8ZM_001167"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0492139.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(12089..12787)
                     /locus_tag="ACG8ZM_001168"
     CDS             complement(12089..12787)
                     /locus_tag="ACG8ZM_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0492140.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(12780..14132)
                     /locus_tag="ACG8ZM_001169"
     CDS             complement(12780..14132)
                     /locus_tag="ACG8ZM_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0492141.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(14355..15755)
                     /locus_tag="ACG8ZM_001170"
     CDS             complement(14355..15755)
                     /locus_tag="ACG8ZM_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0492142.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15916..16653
                     /locus_tag="ACG8ZM_001171"
     CDS             15916..16653
                     /locus_tag="ACG8ZM_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0492143.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            16778..17257
                     /locus_tag="ACG8ZM_001172"
     CDS             16778..17257
                     /locus_tag="ACG8ZM_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0492144.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            17254..18021
                     /locus_tag="ACG8ZM_001173"
     CDS             17254..18021
                     /locus_tag="ACG8ZM_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0492145.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            18251..18820
                     /gene="lepB"
                     /locus_tag="ACG8ZM_001174"
     CDS             18251..18820
                     /gene="lepB"
                     /locus_tag="ACG8ZM_001174"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0492146.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(18924..19526)
                     /locus_tag="ACG8ZM_001175"
     CDS             complement(18924..19526)
                     /locus_tag="ACG8ZM_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0492147.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            19632..19862
                     /locus_tag="ACG8ZM_001176"
     CDS             19632..19862
                     /locus_tag="ACG8ZM_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492148.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            19853..20101
                     /locus_tag="ACG8ZM_001177"
     CDS             19853..20101
                     /locus_tag="ACG8ZM_001177"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0492149.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            20207..20791
                     /locus_tag="ACG8ZM_001178"
     CDS             20207..20791
                     /locus_tag="ACG8ZM_001178"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0492150.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            20794..21867
                     /gene="prfA"
                     /locus_tag="ACG8ZM_001179"
     CDS             20794..21867
                     /gene="prfA"
                     /locus_tag="ACG8ZM_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0492151.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            21860..22699
                     /gene="prmC"
                     /locus_tag="ACG8ZM_001180"
     CDS             21860..22699
                     /gene="prmC"
                     /locus_tag="ACG8ZM_001180"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0492152.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            22692..22871
                     /locus_tag="ACG8ZM_001181"
     CDS             22692..22871
                     /locus_tag="ACG8ZM_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492153.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            22893..23918
                     /locus_tag="ACG8ZM_001182"
     CDS             22893..23918
                     /locus_tag="ACG8ZM_001182"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0492154.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            24093..25337
                     /gene="glyA"
                     /locus_tag="ACG8ZM_001183"
     CDS             24093..25337
                     /gene="glyA"
                     /locus_tag="ACG8ZM_001183"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0492155.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            25490..26437
                     /locus_tag="ACG8ZM_001184"
     CDS             25490..26437
                     /locus_tag="ACG8ZM_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0492156.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            26571..27200
                     /gene="upp"
                     /locus_tag="ACG8ZM_001185"
     CDS             26571..27200
                     /gene="upp"
                     /locus_tag="ACG8ZM_001185"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0492157.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(27339..27581)
                     /locus_tag="ACG8ZM_001186"
     CDS             complement(27339..27581)
                     /locus_tag="ACG8ZM_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492158.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            27702..27774
                     /locus_tag="ACG8ZM_001187"
     tRNA            27702..27774
                     /locus_tag="ACG8ZM_001187"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27735..27737,aa:Thr,seq:cgt)
     gene            complement(27893..28531)
                     /locus_tag="ACG8ZM_001188"
     CDS             complement(27893..28531)
                     /locus_tag="ACG8ZM_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0492159.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(28654..29286)
                     /locus_tag="ACG8ZM_001189"
     CDS             complement(28654..29286)
                     /locus_tag="ACG8ZM_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0492160.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            29637..29936
                     /locus_tag="ACG8ZM_001190"
     CDS             29637..29936
                     /locus_tag="ACG8ZM_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0492161.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10218 a         5271 c         6037 g         8468 t
ORIGIN      
        1 gtaaaaaagg ttgattcctc atagattaag ctttaagctt ggactgtaag aatctgatag
       61 aaaagcagga gaaaaagatg tatccacaca cgaaacagct tattttgaaa aagatggaag
      121 aaggatttta tccaggtgta gcagtcgcct tcataaaaga aaatcaagta gaaacattta
      181 cagccggctt tgcccaagtc gttcctgaaa agcagctgat gacagaagat ctgttgtttg
      241 atgcagcctc actgacgaaa gtcgttgcaa ccacttcatt agttttacgt ctattagaag
      301 atggaaagat agaactcgat caaccgcttc ataaatatct tcctgaattt gaaaatcaga
      361 caatcacttt gcgtcattta ctgacacata cttctgatat caaaacatgg ataccaaaca
      421 gagataagct atctcaggaa gaattgatcg ccgcttattt gaagctagaa ccaggtgagc
      481 aactaggaaa agaagtaaaa tatacagatg ccggtacgat tcttcttggg ttgatgctag
      541 aggaaattta ccaagaaact gcagtggaaa tctttcaaga acaagtattg gaaccattag
      601 gaatgatgaa cagcttattt cttccttttc cttccaaaaa aatcgttccc acagaacaac
      661 tgccttcggg tgaggttttg cgtggacaaa ctcatgatcc gaaagcaaga gttttggctg
      721 aacatgcagg taacgcgggt ctctttattg atttgaaaga cagcattact tttgtccaaa
      781 tgtatttgaa caagggaaaa atccgtaatg gtcgtttttt agaagaagag acgattgatt
      841 ctttactgca agatcaaaca ccgaaaaaaa atggctatcg ttctttagga tgggacttaa
      901 aaaaggatgt agaagacggt catgcgattc tttttcatac tggttataca ggtacgtttt
      961 tcttgattga tataaaaaca caagaagcgt tgatattttt gtcaaatcgc gtacatcctg
     1021 tcgatcatcg tgcggagtat ctcatatgtc gagatgaaat tgttgaaaac tttttgaaag
     1081 agaaagcggt attaccgaaa taatgctata atagctatga aaaatgagag gagtttgaat
     1141 tatgcaggaa cctatgtttt taaaaccagt ttttcaagaa aaaatttggg gaggaagtcg
     1201 tttacgttca gtctttggtt ttgatatccc taacgataaa atcggagaag attgggcgat
     1261 cagtgcgcat cctcatggtg tcagtgtagt agaaaatggc gaattcaaag ggaaaagatt
     1321 agatgaacta tggtcagaac acaaggaatt gtttggccat ccttcagaac cagtgtttcc
     1381 attattgatc aagatcttag acgcagaaga tgatctatct gtacaagtac atccagatga
     1441 tgcctatggg atgaaacatg aaggtgaatt aggaaaaact gaatgctggt atatcatcga
     1501 tgctgaacct ggtgcagaaa tcatctatgg tcatcatgca aagacaagag aagaactagc
     1561 tgagatgatc aaagatgggc gctgggatga ccttttgaag aaagtaccag ttaaaaaagg
     1621 tgatttcttt tatgtaccaa gcggaacgat ccatgctatt ggtaaaggaa tcatgatttt
     1681 agaaacacag caaagttctg acacgactta ccgcgtgtat gactacgaca gaaaagatgc
     1741 gcaaggacaa acaagagagc tgcatatcca acaatcgatc gatgtaacaa ctgtccctgc
     1801 gaaaacacca gaattacaaa tcaaagaagt ccgaaaaggg aattcatcga tcgtcactta
     1861 tttagaaact gagtttttca atgtatatga gtgggatatt aaaggaatca catctttcaa
     1921 aaaacaagcc ccttatacat tgatgacggt aatcgaagga gcaggagacc ttgtcgttga
     1981 cggaaaaaca tatccattag aaaaaggaac aagctgtatt attccaaatg gcgtttctga
     2041 gtgggcggtc caaggtgaac tagcaatcat tgcgtctgaa ccaggcaaat agttcccaga
     2101 tagctgaaag cagcagacgg tttttcgttt gctgcttttt ctacataaaa tccgtccatc
     2161 aataaataga agtctggtga aaaagatgga atacaaatgg aaagcaaaca acagcagtcg
     2221 cgtgtctatg aaacaactga gaaaagctgg acatcactta aatgaaatga tcatgccaaa
     2281 tctaagtctg tttatcgctt ggggaatctt gtcattagca acaaaaggtg tgtctggcga
     2341 tttgcacacc acattagaag aggtatgcca ttggatgatc cagctgcttt tacccatctt
     2401 gatcagttat tcaggcggga aatcggtagg gggacaaaaa ggggcagtcg ccggtgcaat
     2461 tgcttcttta gggttgatcg ctcgttccaa tgctccacaa atcgtaggag cgatgattat
     2521 cgggccgctt gccggcatcg tatatgaaca atttgtttct agattccatg tgaaattcaa
     2581 ggctggatat gaaatgcttg tcagcaacct actagtagga ctgactggta gcgttatttg
     2641 tataattgga atcgttttta ttgaaccagt tttaaatagt gttcacttgt tgctcgcctc
     2701 cgttgtgtct tggctagtca caatgaatct gttaccactt gtcagtctga tcttagaacc
     2761 tctaaaagta ctatttttca ataatgcaat caatcatggt gtattgacac ctttaggaat
     2821 cgaattctca caaacagtcg ggtactcttc cttatttttg atggaggcaa atcctggacc
     2881 tggtttaggg atcttacttt ctatcctttg ttttgcagaa aaacaggaaa aagtaaatgc
     2941 gagtggagca ttgatgatcc atgcgattgg cgggattcac gaaatctatt ttccatttgt
     3001 cttgctgcga ccgtatctat ttttagctgt aatggctggt ggggcaagcg gcacgttgat
     3061 atttcaatgg ttagatacgg gattagctgc acctgtatcg cctgggtcac tactcgtgat
     3121 ttttgccaat gcgccttatc cacaactagc tggggtaagt atagggatag caatcagtac
     3181 atttgtgacc ttcatttgtg cagcgatact attgaaatta ccaaataaag aaaaagagaa
     3241 caaaacggtc aaagaaaggg gagaaatctt ggaaccatct atcattcatt ccgtcatttt
     3301 tgcttgcgac tcaggtatgg gatcaagtgc tatgggagcc gccctgctga aaaagaaatt
     3361 agcagcagaa aaaatcgatc tttctgtcgg atacagctct atctatgaat tgaataacca
     3421 agtaaatcag ctggttatcg tgcaacatga actagcgggt cttgcaagag aaaaagtacc
     3481 tcaaagtcaa atctatgtac tggatcactt tttggaaaca gatatcttgc tgtcacgaat
     3541 aaaagaagcg atccaatttc caaagcggga agaaaaaaaa gagatagcag atatcacaga
     3601 acaaaaaacg tcgcctgcta atattgtatt tttgtataaa aacaacagac gcggctcaca
     3661 aactatgggc atgacgattt taagtcaatt agcaaaaaaa gcgaaaaaac agtttgttat
     3721 ctcaaaagaa tcaatagaaa aaatcaaaat ccaagaagaa acgatttata tcgcacaaag
     3781 agaactagct gtaactgaac agctagaaga aaagatccct cggctagttc tagttgaaca
     3841 ttgggtagct acgaatgaat acgaaaaact cgtgaaggga gaaatataag atgtatttat
     3901 cgcccagaga aaaacaatta ttagcagaat ttctcagtag cccgtcacct gtatctatcc
     3961 aaaaaatgat gaatttattg aaggtcagta aacggactat ttaccgcgaa ttagataatc
     4021 ttgaagtctc attgaaatcg gtaaatgctt ctttgaaaaa agcagctaga ggctcatttg
     4081 tcattgaggc atcagaagaa gtattagagc aattaaaaga aaatacagat gggaaaagta
     4141 atgacttatc cactgctaaa cgacaacacg cgattttagc cgagttgtta ctgacgacag
     4201 agccgttgag tcttgctcat ttcatggaat catatcgcat cagtaatacc actttttatg
     4261 cagatatcaa acagttggaa gaaagtattc gccaactgcc tttggaaatc attcggaacc
     4321 agggatacga gatcgaagga ccggaaaaat accgccggct tctgacagcg aatgtattag
     4381 aattagaaat caatgaatat gaactttttc atagtatttc tttcgatgca tcactgaatt
     4441 atttttttcg attcgttgat ccgcaacatc tatctcttgc acgcaaagta atcggggaag
     4501 aattgatcca aaaaaagacc aatctaagtg atagaaagct tgaacatttg gttttgatgc
     4561 tgacattgac gatggagcgt gtgacgaaaa atcatttact gacaaatgag acatataata
     4621 gtctggcaaa caaagaatta ctacagacag ctaaacgatt gttttcacgg atcgcttctg
     4681 agacaaaaca actttatcca gtaaatgaga tcgtcttttt tgcaggatta ttaaatgact
     4741 tcacgaactc atttgatcag aatttctttg aagagacgtt cgatacacag cttgcttatt
     4801 tagtaaaaca actgattcaa gaagtaagtg aagagacgaa tgtccatttt tatgaagatg
     4861 aaactttgta caaaatgctg ttgactcatt tatctggtgt gttttcaaga gccgttttac
     4921 aagaagagac actgaccaat ccgattttag aaaaaatcat ggttcaatac gaggaagttg
     4981 cgaatgcaat ccgtaaagca gtagagcgga tttttcaaga aaaaaattat ccgaagaaga
     5041 gatcgcttat atggtccttc attttgcgaa ttcattggaa cgaagtccga aagaaagcaa
     5101 tatcagtatt gcaggatttt cgccaagtgg attagcttct actagcatgt tggaactacg
     5161 attgaaaagg tattttccct tcatccaaca aattcatttc tatcggattg ctgatttaaa
     5221 aaaaatcgat ttacaagaag aatatgatgt tgttatttct acatctattt taccaggata
     5281 cggtggaaaa tatattttag tctccccgct actgctagaa gatgaagtca agcagctaaa
     5341 agaagctttt tcgcatattg agaagaaaaa aaggcaaaaa attacgtata acagcaataa
     5401 cgagtggatg atagaagata cttatgagga aacaatgcat ttcatggatc aaatcaatca
     5461 attactcggt cgttttttta tccaagaact ggataatcct gagacgattt ccgaaacgat
     5521 tcgcagctta tttccttatt ttcctgaagg actagtaacg gatgaagagg tggttcatcg
     5581 ccggctgctt aagcgatatg aacaagcacc tatcggtatt cctcatacaa atatggggtt
     5641 attccataca tccagctcat tgatttctaa accgatcttt tgtattttca atttgaaaca
     5701 tccgttgacc attgaaggga tggatagaca gccagtcgat ttgtcccgta tgttagttat
     5761 gctggcgcct tcgccaatcc aagaaaaaga cgggattctt ttaggaaaga tcagtggagc
     5821 aatcatcatg aatgacttga acaccgagat cttccgctca ggaaataaag aaatcgttta
     5881 tcagttgtta gcgaaaatcc tgattgaaga aataaagaaa tgaaggtgaa aagatgatac
     5941 agctaccagc agaaaaaata ttgctcgatc aatcctatca aaattggaaa gaagcgttga
     6001 ccgagatcgg ccagtggatg acccaacaag ggggaatcaa accggcatat atccaatcaa
     6061 tgatcgaaag acaagaaaaa gcgagtgtct atatcgggaa tttcgtagcc ttgccacatg
     6121 ggactgacaa agatgaagaa gtagtagaag aaggaatcta tttagtccaa gtgcctgatg
     6181 gtgtggactt tggaacaaaa gaggaaccaa aaatcgcgac actcttattt gtagtggctt
     6241 taaaaaaaga acatcagtta gcggtgctcc aagatttagc ctttttctgt tcagacatcg
     6301 accaagtaat ggcgctcagt gatgcggaaa ccattgaaga agtccaaatg atttttgaac
     6361 atggacatat ataaccaatg ttcacagtaa aaaaagctgc cttgcatcaa gtaataactt
     6421 caaccaagat aaaaataaga ggagctattt ttgttgggag gttcttatta ctgcggatgc
     6481 aagcttttga aaactattta ttctagaaaa ccgtttattt gaaatagggg acggtgacaa
     6541 ggtttttgtc accgccccta ttatttttgt gttttaatag ctctttttta tatagatagt
     6601 gcccatacct atgaataata aaacgatact ccatatacca agtgtcaaga taccattcat
     6661 gaaagtaacc tgttctgaag ctagtggcga gaaaatatct cctcctatga tcacattttg
     6721 aaaatcaaca taagaagttg gcaagtaatg actgattgat tctatccatc caatcgaaga
     6781 aaacaagcga tactctgcta agaaaagaca aagaagtgta cctaaaagat tgactagaga
     6841 atgttcaaat aaagcagata gaagaaaact gattgtactt aaaaagagca cccacaacaa
     6901 caggaacaga aagctctgga aaagatactc atacatcggc aggattcgta tctcctgacc
     6961 aatgatcgtt cctacaggat aacggaagtc tcccaaacca tttttgatta ttactagtat
     7021 agagacgatc aaaaatggca tcacgagact tagcatagct gcaccataat aaatggtaaa
     7081 acggatgcta tgtttcttta ccatactatc cagtaataga ttgactagag agattacctg
     7141 ctttcttttt tcataagaga tgatggaaga aagaaacaag gcggttatac ataaaatcaa
     7201 aaaactagaa atcattcctg aaaaaatatt ttcgtagtaa tttgctaaag agagatgcgc
     7261 tggcaagtca aaaatagaat atctttggat ctgatgttct ttcatataac ggagcaactc
     7321 gacacgtttt tgttgttcaa tgattggaat tccttgtctt tgaccagaaa tcagtcgatc
     7381 cagtatatct ttttcataat gataagttgc ttcgaccatt tgctggacat tccctgtgtt
     7441 ttttgcatgt aagattgctt cgatcagttt gttgcttttc tctaaacttt ccacaaattc
     7501 tttctcattc tcatcttctt tcaacgtttc aatcaaacgt tgcatctctt catagttatg
     7561 attcagttca tcgaattctg tataagcaag tttatgatca aaaaaaagtg ggaatgcaaa
     7621 gagcacgacc aacaatagag gaaataaaaa gtttactttg ttttttagta agagattgcg
     7681 ttcaaatgta agtaaagaca tagttacacc tcatataatt tacgataaat agctaaaatc
     7741 tcttccatat ttgaggattc tgtgacgatt tctccatttt ttagaaaaca tgctttagaa
     7801 caaatcgatt ggatattcgc gatagcgtgg gaagaaagaa gaatcgtctt cccctctttc
     7861 tgccattgtc tgatgaggct attcaagata tcaatacttc ctgggtctaa tccgttcaac
     7921 ggttcatcga ataacatgac agatgaatca ctgatcaagt aagcagcaag caagacatgt
     7981 tgtttcattc ctagagacat ttttccaatc ggcaaagtaa ggtactcagt catcttcaat
     8041 cgttggaaaa cagtttggca ttctactttg ctctgccaat agtatttgat caaagtcaaa
     8101 taatcaacag ccgatagata gtcggataac tgattcgttg attctagaaa aaagaaagac
     8161 cggcgataat ctgtataagc agtaggagag atgttgtgaa gggacaattg atcataagtt
     8221 gttggcagca gttgggcaat gccattcagc aaagttgttt tgccaaatcc gttcggggca
     8281 atcaaagcag ctacttctcc tgattctact aaaagattca attgtcgaat cacacgtttt
     8341 ttaccacgtt tcatgttcat gtttttgatc agtaatgtca tagataggcc tccaatataa
     8401 aatttaataa cataatagaa tggaaaacat ctaaattaaa tgctaacaaa agttattgtt
     8461 tgcatattat aaaaaaatta aaataacagt tttatttaaa attgaagttt tctgataccg
     8521 aaatcttttt attttgctga catactcaaa cctttggcac aaagtttcgt gccaaagtac
     8581 gactatttct ctttttagta agcggataca attagagtat caaaaggaga gagggagaga
     8641 caaaatggaa cggacgattc ccaaaaacaa catttcgcta aaagcgaggg tacaaaaatt
     8701 aggcagtact ttatcaagca tggtcatgcc aaatattgga gcactgattg cctggggtgt
     8761 attaacggct ctatttatcc cagatggtta tttaccaaat gaggctttcg ctaccatggt
     8821 aagcccaatg ctaacttatc tgattccttt attgatcggt tatactggcg gaaaagtaat
     8881 tgctggtgac cgcggatcag ttgttggggc aattgcaacg atgggtgtga ttgtcggaac
     8941 agatattcca atgatgctag gcgcgatgat catgggacct cttggtggat atgccatcaa
     9001 gaaatttgat cagctattcc aaaaacggat caaatccggt tttgaaatgc tagtcaataa
     9061 tttttcagca ggtcttatcg gttttggatt agcgttatta ggtttttctg cgattggtcc
     9121 agttgtcgat gcattgactc aagcaatggc aaaaggtgta gagatcattt tatctgccca
     9181 tttgattcca ttgacaagta tttttattga accagcaaaa atcttatttt tgaataatgc
     9241 aatcaatcat gggattttga caccattggg aactgaacaa gtagcagagg ctggaaaatc
     9301 tattctattc ttgcttgaag caaatcctgg tccaggactt ggcgtattac ttgcttttac
     9361 tttgtttggc aaaggagcag caaaatcttc agcaccagga gccatgatca ttcacttttt
     9421 aggcgggatc catgaaatct attttcctta cgttatgatg aaaccgttat tattcttatc
     9481 agttataggc ggaggaatgt caggaagttt cattttccaa ttgatggatg ctggactaag
     9541 agcgccagct tctccagggt cgatcattgc gattctagcg atgacaccgt tcaattctta
     9601 ccttcctgtt attttgggcg tgattgttgc cgcagctgtt tcgtttggta tttctgcagt
     9661 aattttgaaa gcggatgcga aagatacaaa tgaagacttc gaaaaatctg ttcaagagac
     9721 aaaggcagct aagcaagctt ccaaaggtgc ccaaggtatt cctcaaggag ctacattctc
     9781 tggcgtacaa aaaatcattt ttgcttgtga tgcaggaatg ggatcaagtg cgatgggcgc
     9841 ttcgatcttg agaaaaaaag tacaggaagc agacttgcct caaacggtga ccaatcaagc
     9901 aatcagccaa ctgacagatg actcgaatac attgatcgtt actcaggtcg aactacaaga
     9961 aagagcaaaa caaaaagcac cgagcgcgcg ttttgtctca gtagaaaact ttttgaattc
    10021 acctcgatat gatgaaatca ttcaagaatt aagcagcaag cagaaaacat cagtttctga
    10081 aattgagaat gagccagcag aagagtttct ttcagcaaac atcaaacaaa tcaaagagat
    10141 tctacttgtc cacgatgatc gttccggatc agcaacaatg gggatgaccg tgctcaaaga
    10201 tatattagac aagaaccatt taactatccc gttaagaaaa gtcaatatcc aagatttaaa
    10261 agatgaacct gattcactga tcgtagccaa agaaagttta gcggaaaaag tacagatggc
    10321 tgcacctcaa tccattcatt tatcagtcaa cagcatggtc aatccgcaaa aatacgaatc
    10381 gattgcacaa tatttgaaac agacagctta agggaggaaa acagcatgca aagtttgcca
    10441 ttcgacatga tcgagctcaa tcgatctttt aaaaccaaag aagaagcgat ccgctattgc
    10501 ggtcagatgc ttgtagaagc agggtgcgtt gatgaggaat atatcgatgc catggtagat
    10561 agagacaaaa tgttatctgt ttatatgggg aattttatcg cgattcctca tggaacagat
    10621 gaagcaaaaa aatatgtgaa aaaatccggc atctgtgtcg ttcaagtacc agatggtgta
    10681 gattttggta ccgaagaaga agaaaagatt gcgactgttt tgttcggtat tgcaggagtc
    10741 ggagatgagc atttgcaact tgtacagcaa atcgcacttt attgcagtga tatggacaat
    10801 gttgtccagc tagcagacgc gctcagcaaa gaagaagtaa cggaaaatct agcaattgct
    10861 taaggagaga gaaaaatgag agcagtacat tttggtgcag gaaatatcgg tcgaggattt
    10921 attggagagg ttttggcagc taatggattt gagattactt tcgtagatgt gaatcacacg
    10981 atcattgatg ctttaaatga aagaaaagaa tacgaaatcg aattagcaga tgaatcaaaa
    11041 gaaaaaatca ctgtaaaaaa tgtcaaagga atcaacaatc aaacaaatcc agaaaaagta
    11101 atccaagaaa tcgctgaagc agatttagtt actaccgcca ttggcccgaa tatcttacct
    11161 tttattgctg agttgattgc aaaaggtatc caagaaagag aaaaggaagg aagcacaaca
    11221 tctttagata tcattgcctg tgaaaatatg attggcggtt cagcatttct agaaaaagaa
    11281 gtgtataaat atcttccgga aacatcattt actgacaaat atatcggctt tccaaatgca
    11341 gcagtcgatc gaatcgtccc attacaacac catgaagatc ctttattcgt ccaagtggaa
    11401 cctttcaaag aatgggtcat tgacgatagc caaagaaaaa ataaaaaaat ccaattaaaa
    11461 ggggttttat atgtagatga tctagaacca tatatcgaac gcaaactatt tagtgtcaat
    11521 actggtcatg cgactgtcgc ttatacaggt gccttgttag gttaccagac cattgatgaa
    11581 gcaatgcaag atgcgttagt tgtggcacaa ttaaaatcag ttttgcagga aacaggaagt
    11641 ttgctgattg ccaaatgggg gtttgatgca gagcagcatc atgcgtatat tgaaaaaatc
    11701 attcatcgct tccaaaacaa aaatatctct gatgcgatca cacgtgtcgc tcggacacca
    11761 ttacgtaagt taggttatca agaaagattt acacgtccag tgagagaact acaagaacat
    11821 aacttgactt gcccacattt gacggcaaca atggggatta tttttaatta ctatgatcct
    11881 gaagatgaac aaagccgtca attgcatgaa atgaagatcc atgaaaactt ggaacaattg
    11941 attcaagaag tcacaggaat caatgatccg aaaacaatcg ggaatatcaa gcaaaacgtg
    12001 aaccgttatg ccaagcaagt cgcttaaaat aggatacgaa aaaggggaat gtgacgaaat
    12061 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
    12121 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
    12181 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
    12241 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
    12301 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
    12361 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
    12421 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
    12481 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
    12541 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
    12601 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
    12661 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
    12721 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
    12781 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
    12841 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
    12901 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
    12961 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
    13021 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
    13081 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
    13141 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
    13201 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
    13261 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
    13321 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
    13381 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
    13441 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
    13501 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
    13561 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
    13621 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
    13681 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
    13741 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
    13801 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
    13861 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
    13921 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
    13981 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
    14041 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
    14101 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
    14161 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
    14221 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
    14281 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
    14341 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
    14401 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
    14461 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
    14521 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
    14581 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
    14641 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
    14701 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
    14761 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
    14821 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
    14881 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
    14941 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
    15001 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
    15061 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
    15121 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
    15181 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
    15241 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
    15301 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
    15361 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
    15421 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
    15481 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
    15541 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
    15601 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
    15661 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
    15721 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
    15781 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
    15841 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
    15901 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
    15961 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
    16021 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
    16081 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
    16141 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
    16201 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
    16261 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
    16321 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
    16381 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
    16441 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
    16501 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
    16561 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
    16621 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
    16681 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
    16741 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
    16801 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
    16861 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
    16921 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
    16981 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
    17041 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
    17101 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
    17161 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
    17221 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
    17281 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
    17341 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
    17401 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
    17461 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
    17521 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
    17581 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
    17641 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
    17701 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
    17761 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
    17821 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
    17881 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
    17941 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
    18001 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
    18061 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
    18121 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
    18181 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
    18241 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
    18301 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
    18361 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
    18421 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
    18481 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
    18541 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
    18601 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
    18661 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
    18721 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
    18781 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
    18841 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
    18901 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
    18961 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
    19021 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
    19081 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
    19141 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
    19201 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
    19261 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
    19321 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
    19381 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
    19441 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
    19501 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
    19561 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
    19621 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
    19681 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
    19741 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
    19801 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
    19861 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
    19921 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
    19981 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
    20041 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
    20101 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
    20161 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
    20221 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
    20281 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
    20341 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
    20401 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
    20461 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
    20521 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
    20581 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
    20641 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
    20701 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
    20761 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
    20821 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
    20881 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
    20941 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
    21001 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
    21061 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
    21121 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
    21181 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
    21241 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
    21301 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
    21361 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
    21421 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
    21481 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
    21541 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
    21601 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
    21661 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
    21721 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
    21781 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
    21841 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
    21901 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
    21961 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
    22021 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    22081 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    22141 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    22201 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    22261 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    22321 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    22381 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    22441 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    22501 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    22561 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    22621 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    22681 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    22741 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    22801 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    22861 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    22921 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    22981 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    23041 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    23101 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    23161 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    23221 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    23281 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    23341 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    23401 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    23461 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    23521 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    23581 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    23641 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    23701 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    23761 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    23821 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    23881 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    23941 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    24001 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    24061 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    24121 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    24181 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    24241 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    24301 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    24361 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    24421 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    24481 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    24541 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    24601 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    24661 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    24721 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    24781 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    24841 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    24901 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    24961 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    25021 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    25081 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    25141 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    25201 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    25261 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    25321 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    25381 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    25441 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    25501 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    25561 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    25621 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    25681 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    25741 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    25801 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    25861 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    25921 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    25981 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    26041 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    26101 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    26161 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    26221 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    26281 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    26341 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    26401 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    26461 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    26521 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    26581 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    26641 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    26701 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    26761 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    26821 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    26881 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    26941 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    27001 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    27061 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    27121 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    27181 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    27241 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    27301 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    27361 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    27421 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    27481 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    27541 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    27601 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    27661 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    27721 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    27781 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    27841 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    27901 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    27961 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    28021 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    28081 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    28141 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    28201 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    28261 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    28321 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    28381 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    28441 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    28501 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    28561 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    28621 ttgctaaaaa tttatcttat atattcattt tgattatact acttttattt gtaaaaattt
    28681 tctagcaaaa ccaagtgctt gcaactgatt aagcgtacaa ttttttagat gttccgagcg
    28741 tacttcaatc atttcaaagg agttgctact taaatcgata ttttttaaat cagtatcaaa
    28801 aaagctagca ttttctaaat cattagtaga aaacattatt ttagtgaggg aacagtctac
    28861 aaaatctgcg cttgataaga cagagtgctc aaaggtactt tcttttatgt ttgaaccaga
    28921 taaatttaca tagtccattt tacaattctc gaataacgtg ttcccaatat aactttccat
    28981 caatttagta cctgttaatt tacaattgac aaatttcacg cgatgcaaca tgctttttgt
    29041 caatatacag cctgaaaaat cacagttttc gaatatacag tcggtaatct ccattctttg
    29101 aaaatcattg gaagcaaaaa atgcatactt gaaaatagat ttgtaaataa ttggatggca
    29161 gattatttgg ttttcgatga cctctttttg tgaaaggata ttatacagtt caaaatcttc
    29221 gttttcatag atatgaaaaa aggatccttt attcaagctt gcttcattaa ctttaggaaa
    29281 aatcatattt aattacctcc attttcataa agtcatatta acaaaagcta aaccaactta
    29341 cattagcaga aaatgagata ggttttcgcc tttttcgata acgatagtgt cgctatctct
    29401 tgaaacgttc ttagaattgc cctgagagac acaaattagg cgtaattcta ataaaaatct
    29461 tagaataaaa atttttactg atttaattta gaatgttttt aaaataaaaa tcgttgccat
    29521 aacagtgtat tgaaaggttt taacaatcca gtaatgattt aaaaaaatct agatgtagcg
    29581 tatactttgt ttatagaatg tagtgggaag aaatatattt ttgggaggaa atcgtgatgt
    29641 ctaaaagtga agaaatagaa aacatggtac taaaattata tgatctcgtt ttaaatccag
    29701 agatcaaaga aaaggaacgt atacttttga tagacgcaaa gacaggctta gaaaaaggtc
    29761 agtattatcc taaagtcata aataatttgg aacgtagttt acgtccgtta gcaatcaggg
    29821 gagaattgtc taaaccagta tcaccgtttt atatggagat atcaacaata ggaaaatttg
    29881 aaaaagaatt aggtcgagga atggcttcgg caccgattac ttttgggcat ttataaaaac
    29941 gagttattca tctcaatgaa ttaaaaagta tagtcaatct cataatgcag gcta
//
LOCUS       DAZKOR010000029        29334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000029 DAZKOR010000000
VERSION     DAZKOR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.029"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..566)
                     /locus_tag="ACG8ZM_001191"
     CDS             complement(132..566)
                     /locus_tag="ACG8ZM_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0492162.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(666..1514)
                     /locus_tag="ACG8ZM_001192"
     CDS             complement(666..1514)
                     /locus_tag="ACG8ZM_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0492163.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            1903..3669
                     /locus_tag="ACG8ZM_001193"
     CDS             1903..3669
                     /locus_tag="ACG8ZM_001193"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0492164.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            3771..5030
                     /locus_tag="ACG8ZM_001194"
     CDS             3771..5030
                     /locus_tag="ACG8ZM_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0492165.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            5177..6478
                     /locus_tag="ACG8ZM_001195"
     CDS             5177..6478
                     /locus_tag="ACG8ZM_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0492166.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            6481..7332
                     /locus_tag="ACG8ZM_001196"
     CDS             6481..7332
                     /locus_tag="ACG8ZM_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0492167.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            7345..8160
                     /locus_tag="ACG8ZM_001197"
     CDS             7345..8160
                     /locus_tag="ACG8ZM_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0492168.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            8311..9477
                     /locus_tag="ACG8ZM_001198"
     CDS             8311..9477
                     /locus_tag="ACG8ZM_001198"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0492169.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            9707..10354
                     /locus_tag="ACG8ZM_001199"
     CDS             9707..10354
                     /locus_tag="ACG8ZM_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0492170.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            10438..11382
                     /locus_tag="ACG8ZM_001200"
     CDS             10438..11382
                     /locus_tag="ACG8ZM_001200"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0492171.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            11517..12968
                     /locus_tag="ACG8ZM_001201"
     CDS             11517..12968
                     /locus_tag="ACG8ZM_001201"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0492172.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            12989..13825
                     /locus_tag="ACG8ZM_001202"
     CDS             12989..13825
                     /locus_tag="ACG8ZM_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0492173.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            13825..14484
                     /locus_tag="ACG8ZM_001203"
     CDS             13825..14484
                     /locus_tag="ACG8ZM_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0492174.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            14610..15299
                     /locus_tag="ACG8ZM_001204"
     CDS             14610..15299
                     /locus_tag="ACG8ZM_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492175.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            15305..16261
                     /locus_tag="ACG8ZM_001205"
     CDS             15305..16261
                     /locus_tag="ACG8ZM_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0492176.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            16305..16817
                     /locus_tag="ACG8ZM_001206"
     CDS             16305..16817
                     /locus_tag="ACG8ZM_001206"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0492177.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(17073..17621)
                     /locus_tag="ACG8ZM_001207"
     CDS             complement(17073..17621)
                     /locus_tag="ACG8ZM_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0492178.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(17710..18891)
                     /locus_tag="ACG8ZM_001208"
     CDS             complement(17710..18891)
                     /locus_tag="ACG8ZM_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0492179.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(19127..20635)
                     /locus_tag="ACG8ZM_001209"
     CDS             complement(19127..20635)
                     /locus_tag="ACG8ZM_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0492180.1"
                     /translation="MKKNGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            20794..20985
                     /locus_tag="ACG8ZM_001210"
     CDS             20794..20985
                     /locus_tag="ACG8ZM_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492181.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            21188..22105
                     /locus_tag="ACG8ZM_001211"
     CDS             21188..22105
                     /locus_tag="ACG8ZM_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0492182.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            22098..23171
                     /locus_tag="ACG8ZM_001212"
     CDS             22098..23171
                     /locus_tag="ACG8ZM_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492183.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            23319..23837
                     /locus_tag="ACG8ZM_001213"
     CDS             23319..23837
                     /locus_tag="ACG8ZM_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492184.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            23818..24210
                     /locus_tag="ACG8ZM_001214"
     CDS             23818..24210
                     /locus_tag="ACG8ZM_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0492185.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            24286..25221
                     /locus_tag="ACG8ZM_001215"
     CDS             24286..25221
                     /locus_tag="ACG8ZM_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0492186.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            25354..26133
                     /locus_tag="ACG8ZM_001216"
     CDS             25354..26133
                     /locus_tag="ACG8ZM_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0492187.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            26126..26443
                     /locus_tag="ACG8ZM_001217"
     CDS             26126..26443
                     /locus_tag="ACG8ZM_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0492188.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            26687..28297
                     /locus_tag="ACG8ZM_001218"
     CDS             26687..28297
                     /locus_tag="ACG8ZM_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0492189.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            28454..29302
                     /locus_tag="ACG8ZM_001219"
     CDS             28454..29302
                     /locus_tag="ACG8ZM_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0492190.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         9555 a         5210 c         5800 g         8769 t
ORIGIN      
        1 ataattgtgg aaagcaaaga aatggattgg cataagataa ataagagaaa gcacatgaaa
       61 cagacgcgct ttctcgaata gcaaacatat acaaaaaata tcagagacaa aaaataatct
      121 catcataaac attacatata tttctcaaaa agttgttttc tttcttcaaa aattacttca
      181 cgatggacct gatgaagcat ccataaataa ccaaaaggat ctctgaacat tgagttagtc
      241 acaccaaagt ccatcatttc tgtcatttct tgaatctcta cacagccatt tcgtaatgct
      301 ttattatgtg tgtcctcgat atcagagacg atgatattga accagatcgt atttggatgt
      361 tcagcagtag gagcagtcaa accaagagac gtgttttcat ccagcatatg gaatctcgta
      421 ccatacaagg taaaaatgac ttcattattg cctctagggt aatctgttgc ctcaatcagt
      481 tgtatttcat caaagattgt ttgatacagt tctagtgctt tcaaactatc atttaataca
      541 aaatcgattt ccgttccttt atgcataaag atctcctctc cttttcagtc gaacagttac
      601 tagattattt tattatacca gctaaagaaa acgcattcaa aaaaatagcg gatttactta
      661 ccaactcatt cgattggatg ataagtaaat ccgcgtccca ttacttctgc tacgctgctg
      721 acaatgacaa aggcacttgg atcgatttct tctatcaatg gtttcattcg ggaaaattca
      781 tgatcagtca caacgatcaa taaaacctct tttgactttc gattataccc gccttttgcc
      841 tctagcaggg taaggccacg tccaatctca gcacttaagc gaaggcggat gtcttccaaa
      901 taatgttcac tcatgaccat aacagatttc tttcgattga tacctgtttc gatataagtc
      961 atgacaattg aagtgattgc aatagataaa atagcataaa gaaactcttc tattccaaaa
     1021 ataaacagat taagagaaac aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc
     1081 aagtggaaat attttttaaa aatgagggga gggatcgtcg taccgccgct agaagagttg
     1141 tttttatata aaatcgcaac tcctaaagca aaaatcgcac ttccaaaaat gacagacaac
     1201 aaccgatctg aagtaatcat tgtttctgga actattgcaa tgattaaagg aaaaaccaag
     1261 cttccgaaaa gtactttaaa aaagggtttt ttacctaaaa atagaagagc tagtaccaac
     1321 ataacgatat tcaacactaa aataactata gctcggtcaa agcctaaagc agcttccagt
     1381 aatattccta gaccgctgac acctccagca gcaatatgat gaggccctaa aaacatattg
     1441 atagcgactc ctaatatgaa gagggcaaaa ataatcttaa tgtatgtcca tataaattgt
     1501 ctcgtttctt tcatttattc acttccttaa ttcattaata atactcttat cttattataa
     1561 cttacttttc accttaagtt aactttaatt aataaaaaaa ttaggctagt ctaaattatt
     1621 atgtttcatt cttctagaat atacttctac gccaaaaact gttaaaaaac ttttacttta
     1681 gaaaattgga gaaattgatc gcttaagata aattcaggac actaaatagt acaggaagta
     1741 gactaaaact tttaaaagtt ttaagtctat ctatttggca agtttaaaaa ataaaaattc
     1801 ggtatcaaaa aactgttacc gataacattt ttgaaagcgt tgtcttttgc ttctctatca
     1861 cttatagtga tgacgtcata ttaaaacaca aggaggaaat caatggatac agctgcaatt
     1921 tatcatcgac cggaaagtga atatgcattt ctatatacga atacaagatt tcgtattcgg
     1981 cttcgtacta ggaaaaatga tatcaaaaaa gtatatgtgc tatgtggaga tccatatacc
     2041 ataacaactg aaaaatggta tcaaaaacaa cgaccaatga aaaaatgtct aagtaccaat
     2101 gttcatgatt attgggaaat tgaagtgacg agcgaaacaa gtcgcttaca gtatgcgttc
     2161 catgttgttg gggaagataa cacggactgt ttttacggtg atcaaggaat ttttccttat
     2221 cgagaaaatg tgattactga accaaatttt tattttcgaa taccttattt tcatcaaatc
     2281 gatcgattca ctattccaga atgggtaaaa gaaacagtat ggtatcaaat ttttcccgaa
     2341 agatttgcaa acggggacaa agcaaatgac cccgaacaca ctttaccttg gggaagtaaa
     2401 gatccagacc gagaagactt tttcggggga gatttgcaag gtgtgatgga tcatttggat
     2461 tacttgacgg accttggcat caatggcatc tatttttgtc ccatatttaa agccacatcc
     2521 aatcataaat acgatacgat tgattattat gaggtggatc ccgcttttgg cgataaaaaa
     2581 cttttaaaaa acttgattga tgaagcacat aaaagaggga tacgtatcat gttcgatgct
     2641 gtattcaatc atatgggtgt ccattcacca cagtggcaag acgttctcaa aaacggagaa
     2701 aaatcgatct ataaagactg gttccacatc cgttttttcc cagtagattc ttatcagatg
     2761 actgacttac cagaaactgc tgaaaatatc ccatatgata cttttgcttt tactccattc
     2821 atgccgaaac taaatacagc aaatccagaa gtgcaaaaat atttgctgga tatcgcaact
     2881 tattggataa aagaattcga tattgatgga tggcgtcttg atgttgctaa tgaagtcgat
     2941 catcattttt ggaaaaaatt cagagaagca gttacagaaa tcaaaccaga catctatatc
     3001 cttggagaga tttggcattc ctctcaagct tggctgcaag gggatgaatt ccatgcagtg
     3061 atgaattatg catttacaga ttcgattaaa gattattttg caaaaaagaa aatcacagct
     3121 tcacaaatgg tcagtggaat gaatcatcag caaatgcttt acagagatca ggtgaacgaa
     3181 ggaacattca acttattaga ttctcatgat actgccagga tcttgaccct ttgccaaggt
     3241 aacaaagaat tgatgaagtc tgtgatggcc tttatgtttc ttcaaaaagg gtctccttgt
     3301 atctattacg ggacagaaat agggatgact ggtgaagatg acccggattg ccgcaaatgt
     3361 atgatatggg agaaggagga acaagatcta gagctattcg gttttataaa agaacttgtt
     3421 tctctaagga aacagttgtc taagattatt tctgaaggtt ctactcagtg gctaatagta
     3481 aatgatcgtg aagataagct ctatttcaca cggaaactag agggacagat catctatgtt
     3541 tactttaatc agtcaaaaga accttgggta gtagaacagg aaaatgaggt gattcttagt
     3601 caaaattgtc agttgttaga agacgggaaa gcagagatac gacaatacgg atttttgatc
     3661 atgtgttgaa aactgttacc ggtaacaaca agaaagcgtt tgcaaatgaa gatttgtgcc
     3721 cattataata agggtgtaac agcacgttga atacaaagga ggaaatgaag atgaaaaaaa
     3781 gaagtgtgaa acagttgggt ttagggttaa tctctttagg aatcttaggc ggattagcgg
     3841 cttgtggcaa tggtaactct tcagcagaag gggaagccga tgaaggaaaa acgttaacag
     3901 tttccgttga ttctggatat aaagattata taaacgaaat aaaaaacact ttcgaaaaag
     3961 aaaacgatgt gaaaatcaag ctagtagaaa aggacatgtt cgatcagttg gaatcattag
     4021 cgttagatgg gcctgctgga aagggacctg acgttatgat ggcagcatat gaccggatcg
     4081 gtgcgttagg acagcaagga catctggcgg aagtaaaact gggaaatgaa gcagcttacg
     4141 atgaaacaga caaagcccaa gtgacttatg atggaaaaat ctacggagaa cctgcagtaa
     4201 tcgagaccct tgttttgtac tataacaaag atttagtcga tacagcacca tcaacgttta
     4261 aagatttgga gaatctatcc aaagacagtc gttttgcttt tgagtcagaa gcaggaaaaa
     4321 atacaggatt tttagctaaa tggactgact tttattattc ctatggttta attgcaggtt
     4381 acggtggcta tgtatttggt gatgatggca caaatccttc agatatcggt ttgaataatg
     4441 caggtgctgt agaaggaatc tcttatgcaa cagattggtt caaaaatgtt tggccaaagg
     4501 gaatgcaaga cataaaaagt gcaggagact tcgccagcca gcaattcatg tccaataaaa
     4561 ctgcagcaat cattgacggt ccatggcaag cgcaaacata caaagaaaac aatatcaatt
     4621 atggtgtagc caaaattcct actctaaata atggacagcc ttatcaaccg tttggcggag
     4681 gaaaaggatg ggtagtcagc aattatgcta aaaacaaaga cctctctcaa aaatggcttg
     4741 attatgtaac gaatcaggaa aatcaagaaa aattctttga aatggtaaat gaaatccctg
     4801 caaaccaaca agctagggaa acagcaaagg ctaaaaacga tgaattaaca acagctgtga
     4861 ttgaacaata tgaaacagct caagcgatgc caaacatccc agaaatgggc gaagtttgga
     4921 caggtgcaga aaacttgatg ttcgatgcag catcaggttc aaaaacacca aaagaatccg
     4981 cagatgaagc agtaaaaaca atcagtgaag ccatagaaca aaaatactga ttaaaatgat
     5041 aaccgtccat ttttataaaa aatggaacag gtgcttcaga taaaagtggc aagagcgaga
     5101 tggaaaatta tcttgctctt gttgccatta tcttaaaagc agtttgcggt ttagcaagtt
     5161 tagagggggc attcagatgc agcaagataa aacaagcaat atcaacgaaa aaaaaatcac
     5221 gctttgttca cttattccag ggatggggca gtttgtaaat ggccaagctt ttaaaggtat
     5281 cgtttttttc gccatattcg tcctattcat tggtcagatg cttctgggcg gatttcatgc
     5341 attggtagga ttggttactt tgggaagtat accaatggaa gatcattcat tatttatttt
     5401 gattgaaggc actttgcaat tcatcattac aatcattttc ttgatttttt acggtttgaa
     5461 tcttttagac gcttatcgag tagcgaagat gcgtcaagag ggcaaaaaaa tcaatcggac
     5521 gatgaaggaa gtcatacata atataggtga tgaaggtttt ccttatctat tgacacttcc
     5581 cgcttatctt ctcatgattt ttacgatcat tttcccagtc ctagttacat tgttcactgc
     5641 atttactaat tatgacttca atcatattcc accagccagt ttaattgatt gggtaggggc
     5701 taaaaacttt tttagtatct tctttttaag ctcatatcgt aatacttttt ttgcagtctt
     5761 ttcgtggacc gtgatttgga ctttatgtgc aacaactttg caaatcaccc ttggtattct
     5821 cacagcggtt gttgctcacc agaaatttat taaattcaaa cgtattttcg gtatcatttt
     5881 cttattacca tgggcggtcc cagcttttat tactatcatg agtttttcga atatattcaa
     5941 cgatagcgta ggtgccatca atactcaagt gattcctttc ataaaccagc ttccttttgt
     6001 agatatcagt gcaatctctt ggaaaacgga cccgaactgg acgaaagtag caattatctt
     6061 agtgcagggc tggttaggat ttccctatat ctatgtgatg gttacaggaa tcttacaatc
     6121 gatttcagaa gacttatatg aagcagcaaa aatcgacgga gcaaatgcat ggcagcgctt
     6181 ttcaaacatc acgcttcctg ctgtcttcct agtggcagcg ccaacattta ttacacagta
     6241 tacggggaat ttcaataatt tctccatgat ctatctgttt aacaatggcg gtcccggaag
     6301 cgtaggcgga ggagctggtt ctactgatat tcttatttcc tggatctata aacttacgac
     6361 tggaacttct ccgcagtatt ctatggcttc agcaatcacg ttgattatct ccgtcatcgt
     6421 tatctgtgtg tcattgctgg tatttaaaaa gacaaatgcg ttcaatatgg gggattaaca
     6481 atgaaaaaat ttcattcgag tgctcgattc caaaaacgac tttctcactt ttttacttac
     6541 ttttttcttt taatactgtc agtcattatc atctatccgc ttttgatcac agcaagtact
     6601 gccttcaagt caggaaatat tgcagctttt agtttagatc tttcaagtga ctggacttta
     6661 ggaaacttta ctcggctatt cagtgaaaca ctttacggga cttggtataa aaatacattg
     6721 atcatttctg tcctaacaat gatcgtacaa gtgactgtag taactttggc gggctatact
     6781 tatagtcgct atcgttttat cggccgcaaa tcaagtttga tgttcttttt agtgattcag
     6841 atggtaccga ctatggcagc attgactgcc ttttatgtca tggctttctt attagaagcg
     6901 ctcgataagt actggttctt gacaatgatc tatataggcg gtggaattcc aatgaatact
     6961 tggctgatga aaggatactt tgacactgta ccaatagatt tagatgaatc tgctaaactt
     7021 gatggggcag ggcatttcag gatttttttc cagattgtat taccgcttgt caaaccgatg
     7081 ctcgccgttc aagcattatg ggcttttatg ggaccttttg gggatttcat gttagctaag
     7141 ttcctgcttc gaacacctga aaatcagact gttgctgtcg gcttacaaac atttatttct
     7201 gatgtgacaa accaaagagt ctctcttttt gctgctgggg caattttgat tgcactaccg
     7261 atttgtctat tattcttctt tttacaaaag aactttgtta ccggcctgac atctggcgga
     7321 acaaaaggat aggagcagag tatcatgcaa acatttccgt taaactattt ttccgcatta
     7381 cgtacgccaa ctcaagtgtt tgcgggacgc aaactattat catggcctaa atttttcttg
     7441 atatttgtat ttttagtttc cttgatggtc atgcctgtga ctctttttta tgccaatcag
     7501 atccaagcga ttccattaga acaatttcta tccgttcatt cgctgatcga tgaacaagga
     7561 acccaaaaat tttctgaact tgaattatct gaaactgggt tacaagcatc gcaacaaacg
     7621 atagctgtga cccctgaaat tttggtaggt gtgtctttat ctgagaaaca acaatccgat
     7681 catggaactt ttattgattt tgagaaggaa caatgggtga tccaacaaaa ggataaatct
     7741 ggtatacgcc gctatacgat gaattatagc ccatctttcc aaccagattc tgtacgtacg
     7801 cccgaagatt tccaacgatt tttagaaaga gaattttatg ccagtaatcg tccaacaatc
     7861 attttatctt atagtttaag tcttggtctt gtcctatttg tcatgacgag tctcatttta
     7921 ttcggggctt cctttttctt gtggatgaca agaaaaagcc aactttcatc cattcataca
     7981 ttcaaagaga gtgcaaattt gatgctgaat gttatgggaa taggttccat ggcagcagca
     8041 gttgtagggt ttatccactt tgactttatc ttgatgctaa gtgtccaaac aacgataact
     8101 gtcttgctgc ttttatgggt ttttgtcaaa acaagattca aagataagcg tgtcgaatga
     8161 caagtttaag cgatttatgc gacaatgaat gtgaaaaaaa tgaaaaataa agtagttttt
     8221 gaaaaatttc tgaaaaaatt aaacttctta gttgattctt ttctttttta gtattaacgt
     8281 aatagggtag tttttttaag gaggaaaatc atgttagaga aaactttata caatcaatta
     8341 ttttcacgtt ctttctcgct ccctgtcgaa gtaaattatt gggacggtac aacgaaacaa
     8401 tacggagata cggacactcc tccccaaatc aaaatcaata tccatgaaga aatcccaatg
     8461 aaagagatta caagtaacgc ttctttggca ctaggagaag cctatatgga tcatcgaatc
     8521 gagatcgagg gaagtatcca agcattgatc aacgatgctt atcagcaagc aaacagcttt
     8581 ttacgaggaa aagattatct aaaatggctg ccaaagaaag aaaaacatac gaaacaaaaa
     8641 aacaaagaag atatccatgc gcattatgac ttaggtaacg acttttataa actttggcta
     8701 gatccaactt tgacttattc ttgtgcttat ttcaatacac ctgaagatac gttggaacaa
     8761 gcacaaatca ataaagtcca tcatatctta gacaaactct ttatcaaaga aggcgaaacg
     8821 ttgcttgata tcggttgtgg ctggggaaca ttgatgttca ctgctaccaa agagtataat
     8881 gtcaaggcaa ctgggatcac actaagtgaa gaacaatacg atttgattac caaaagaata
     8941 aaagaagaac atttagaaga taaatgccgt gtattattaa tggattaccg cgaactaaaa
     9001 ggcgaaacgt ttgatcatat cacaagtgta ggtatgttcg aacatgtagg ttctgaaaat
     9061 cttgaaggtt atttcaaagt ggtaaaagat ttattgaaac caaaaggaac agctttgatc
     9121 catggaatca gccgacaaca aggcggtgcg acaaatgctt ggatcaataa atatattttc
     9181 cctggcggtt atgtccctgg tgttactgaa ttagttgata atattacaaa aaacgatttg
     9241 caactgattg atttggaaag cttacgtcgt gactaccaat tgacattaga acattggaca
     9301 aagaattttc atcaagtcca agatcaagtc acgaaagaaa tgggtgattc cttctatcga
     9361 atgtgggatc tatatttaca agcttgtgca gcttcatttg aatcaagtaa tatcgatgtg
     9421 attcaatatt tgcttgttca acctggaaac aatgacatcc caatgcatcg tgcataattg
     9481 aggcaaaagt actgtttaaa gactatttaa aaagaagcta agttttatga tgctccagaa
     9541 gtgaactttt ttgtctaact tctaggattg tctctaaagc ttagtttctt tttctttata
     9601 ttttcataat aaaagtttgg ttctttaatt tttaaacatg acagaaaatt ttttcttcgt
     9661 tatacttgta atagaatact attttataac aaaggggatc gaatcaatga aaaaaactgt
     9721 tatttatact agtttatttg cagctttgtt tttagctgct tgtcaatcaa ataccaatca
     9781 aggcacatct gggaatacgt cttccagtag cgaacagcgc tcttcacagg tatcggactc
     9841 ctcaacaaaa gcaagcacat cttcgtctag ccagacaaaa agttctagtg aagcaagtac
     9901 tcagtcagat atcaaagtgt ctgcagctcg tgcgatcgaa ttgttccaag ataaataccc
     9961 agatactgca attactagtt tggaattaga tagtgattgg ggatcttact tctacaagat
    10021 agaaggtgtc gatgatcaaa aagaatacga agcaagaatt gatgctcaaa atgaaaaaat
    10081 ggagacagag aatccagaaa ctctagatcg agatgaacaa aatggtcaaa agaaaaaaga
    10141 agatggtctg gatgttgata aattgatcac gattgaggaa gcagcaaaaa tcgccgtcga
    10201 caaagtagga agcggtacgg caacagattg ggatttggat aaagatttag gtactactta
    10261 ttgggaagtc aaagtaaaag acggcaatca aagtacatca gtcaagatca atagccaaac
    10321 aggcgaagta ctgtcaactg aacaagatga ttaagaattt cttcggaaaa caagagttga
    10381 tttttctttt agtcatgata cactagacaa aataacttgt ttgaaggagc gatttacttg
    10441 agtttagaaa cgacctttgc caatcatatc tttgccaatc ctttgatgaa tgcatctggt
    10501 gtccactgca tgactaccca ggaattagat gaactggctc attcagaagc aggagcattt
    10561 atcactaaga gctgtacgat caatgaacga aaaggaaatc ccgaaccacg ctattttgat
    10621 gttcctttag ggagtatcaa ttctatggga cttccaaact taggtttttc ttattatcta
    10681 gagtatgctc ttgcgtatga aaaagtgcag gaaaaccaaa atcaaccgtt atttttttcc
    10741 attgccggga tgagtgtcca ggaaaacttg gaaatgcttg agaaaataga aaaaagtggg
    10801 tttaatggga tcactgaatt aaatttatct tgtcccaatg tgcctggaaa accacaacta
    10861 gcttacgatt ttgaggccac ttatgaaaca ctgaaagaag tattcagcat cttttctaag
    10921 ccgctaggaa tcaaattgcc tccttatttt gattttgctc attttgatca gatggctgat
    10981 atcttgaatc agtttccatt gacctatgtc aatgctatca acagtgtggg caatggtttg
    11041 tatatcgata cagaacaaga agcagtagtc atcaaaccaa aagaagggtt cggcggcatt
    11101 ggcggagagt atatcaagcc gactgctttg gcgaatgttc gcgcttttta tacacgtttg
    11161 aaaccagaaa tccaaatcat tggtacaggt gggattcgga caggacaaga tgcatttgaa
    11221 catctgctat gtggcgcaag catgttacag attggtacag agcttcataa agaagggccg
    11281 gaaatttttt ctagaatcat aaaagaatta acgcagatta tgtcagaaaa aggatatacg
    11341 tcaattgacg aatttaaagg caaattacgc accatttcat agcaaagaga cgaaggtcat
    11401 gattgatctt cgtctttttt tataaaaaac actcgtctgt tcattcctgt gcaagcgtac
    11461 tcattgtatg ttagacgaac ttgtgggaaa ataacagagg caaaaaggag gataatatga
    11521 atgatgcaac agaattagca aaagagctta aagaaaaaga atttagtttt caagaactag
    11581 ttacggatat acagaaaaaa ataaagaagc aaaatcatga attgaatgcg tttgtcgcgc
    11641 ttgaaccatt cgagagtaat tcccacgagc tattgattcc ccagtcagaa aaagggccat
    11701 tggctggtat cccatttcct ttaaaaatgc tcggtcaaga aaaaaaggga tggctagcta
    11761 cttcaggatc tcgtttattt gaaacacacc gagcttctca tacatctaat tatgtgcggc
    11821 aagcagaagc gataggtcta gttccttttg gacaaaccaa tgcgccagag tttggtttca
    11881 aaaatattac agacccagtc atctatggcc cagcaagaaa cccttggaac cttgaatgta
    11941 caccgggcgg atcaagtgga ggagctgctg ctgcagtttc atcaggaatc gttcctttag
    12001 ctggtgctag tgatggcggt ggttctatta gaatacccgc ttcgttttgc ggattgattg
    12061 ggctgaaacc ttcgcgaggg acgatgccag ttggtccata tgcttggaga ggatggcaag
    12121 gagctgctat tgatttcggc ttgacagttt ctatgagaga cacggaggct ttattttatg
    12181 ggatgagaag tatccattcc ggtgcgccat atcaagtatc gccagttgag tggcaaacac
    12241 atccaagaaa aaaacggttg aagatagctt tttgtgttga ttcaccgatc cactcacaga
    12301 ttagtgaaga ggcagtactt gctgtaaaaa aagctgtaga tttccttgga aaagaagggc
    12361 atgatttgac agagattcct tacccactag atggtcgtaa actgatcacg tcttattatc
    12421 agatgaatgg cgcagaaaca gcagcaatga tccgctcgat tgaaacaggc atccagcgag
    12481 ctgtcagaaa agaagaactt gaacccattt cttggacttt gttccaatat ggtcagaagc
    12541 tctctgccgc tacttacatc gattctctgc atgtatggga tgaagcagca attgtcatgg
    12601 aaaacctaca tcaagaatac gatctattcc tttcaccaac gactgcattt accgccccta
    12661 aaatcagcga agatttgcaa agtgatgcga taagagcaca gatggaacac atcagtgatt
    12721 gttctgaagt agaaggttta gacgttgttt cttccatgtt tgaggagagc ttgaagatca
    12781 caccttatac acagttggct aatctgactg gacaaccagc aatcagcttg cctactcatt
    12841 tgacaaaaga aaacatgcct ctaggaatcc aatttatggc tgccagagga agagaagatt
    12901 tgctatttca aatcggctat ctatttgaag caaatcatca gttccattta ccaaatccat
    12961 ggaaatagca gaaacaagga gaagaagaat gaagaaaaaa agattgtttg ctctaataac
    13021 tagtgccttt tttattttaa gcgcttgcgg tacagctgcc gctaatcagg aagataacag
    13081 ctggagtaat gtagaaaaaa agaaagaatt agtagtagcg acttctggca cactgtttcc
    13141 cgcatcttat tataatgatc aaaatgaatt agttggttat gatgtagatt tggcaaaaga
    13201 ggtggctaaa cggttaaatg tcaaaatctc ctttaaagaa tacaacgtgg atggacaaat
    13261 cacttctgtt tcaaaaagag aatcagattt tgctgcgaac gattttggta tcacaaaaga
    13321 aagagaaaaa aaattctccc tttcagagac tttcaaatat tcatttgaca gtatgatcgt
    13381 acgaaaaaaa gatgattcag gaatccactc acttgaagat cttaaaggga aaaaagcagc
    13441 tggcgaaccc aatactagct atatgcgttt agcggaaaaa cacggagcta aattagtcac
    13501 atatgacaat gccacgaatg atcagtattt aagcgatgta gcaaatggtc gaacagatgt
    13561 tattctaaat gattattatc tgcagaaaat ggccgtatct gctttaccag atatccctgt
    13621 aaaaatactg gagaacgttt atttcaatga aaacgaaagt ggttttttat tgaaagaagg
    13681 aaatgatgct ctgaaaaaga aaatcgatgg cgtattaaaa gagatgaaag aagatgggac
    13741 gacaaagaaa atatctgaaa aatattttca aactgatgtt tcagtcaaac cgaaagaaac
    13801 gatggctgag tcggtgagtg agtgatgtac tttcctctaa ttgatttttc cctcatgtgg
    13861 acttcacttc ctttcgtact gcaaggcttg gtatacacat tagggattgg cttcgtgtcg
    13921 tttcttttag gaaatttggt agggttgctt ttgacagttt taggtctttt ggattggctg
    13981 cctctgaatg tatttattcg tttttattta tcgttcttta gaggcattcc cgcactagtc
    14041 ctgctgtttc tgttatattt cgggttacct tatcaattaa gtgccttaac ggcttcagtc
    14101 atctgcttta cgattaccag cagtgcattt attggtgaaa tctatcgagg atcactagca
    14161 ggcgtttctt caggtcagtg ggacgctggt ttagcttctg gtttttcttt ctttggggta
    14221 ttgaaatata tcgttttgcc gcaagctttt cgaatctcag taccggcact cagtaacgta
    14281 gctatggatc tagtgaaagg tacttcgtta gcagcaacaa tcacagtgcc agaaatattt
    14341 caaaaagcaa agatcatagg cggtcggaca ttcgattatt tttctctcta tattttagtt
    14401 gcactgattt attgggtcat ttgtatcggg atcgaaaaag tgcaacagta tttagaaaaa
    14461 cgttctatcc atcagtcaat ctgaaaaata gaaatggtgc tagcgtggct ggcaccattt
    14521 ctattttttg tttcttgtat attctgccta gtattttaaa accatttcat gtaaaatgta
    14581 aatcgaaaat atgttcggag gtagaaaaca tgaagacccc gctaaccatc gaaatagaat
    14641 cctcgttata ccaatattgc atcgagcaag gcggcttggt tgtagaagaa gtcacgatgc
    14701 ctgatgacca aggaattgta gatacgctta gctgcttgac aaaatttgat ggaagccgag
    14761 aatggcgatg ctatgagctg aaagtgacaa aagcagattt tcgctctaca gccaaactgt
    14821 catttgttgg acattataat tattttgttt taccaagtaa actatatgaa gaagtaaaaa
    14881 ctgaaatccc tacaggaata ggtgttttag tgtatcgtcc gtacacggtt gaagaggaga
    14941 tgcctgcctc tggtacattt ctgattgcga aaaaagcagc aaagaaagag cttgctgtac
    15001 cggaagaagc tttgacaaac cgcttcatgg cttctttatt cagggaagta agaaaagcaa
    15061 agcaaatgga gcgaggcgta aaatattttc caaaagatca attgtacaaa gaattgaaaa
    15121 gacgttcaga aaatcaagat ccattttcta acgaacgata ttatgaacgg tttttagagg
    15181 aaatagctag tcaggcaatc aatgagttgc aagaagaagt tatcgcgctt cagcatgatt
    15241 atgaatattt gaggcaacaa cagcagatcc gtcggttgcc tacagagcca ttggagtgaa
    15301 aaatatgtac tatccttatt taagaggaaa acaatttgac ctattagcat taaaagaagc
    15361 actgtcgcga ggattgctct ccaataaaat ccaaccagtg atcgaaccag tacgtgattc
    15421 cgctacgctt aaaaatgtga tcgaattatt tcaaaaaaag caccatccaa tcgctgtgat
    15481 ccaaaatccc caagtaggac aattcaaatt attcgatcag catgtgcaca gttggacagt
    15541 caaagaaaat agctctgtcg tccctgcaca gatcttaacg ccagaaaatc tcagtgaagt
    15601 actggattca cctccagcgt ttcttgtttt cgatggacag cactacccaa aagatacaga
    15661 ggtgtggaaa cagttggcag gagttgattc gaaatttttg ataccagata ctagtcgctt
    15721 tcgcatctgg ctgcccgaaa acaaaattgt gatccgtgac tcttttcaaa cgagaaagca
    15781 tgttgaaagt tatgcagata aaacagatga cttttttagt gatgactatc tttttttcca
    15841 cgcagacgga tatattggat tttctgattt cacgatcgaa ggtagccgtt atttcgataa
    15901 agggttccct agccgagcgc ttgctatcca tctaacgtat attgatgctt acggcaatat
    15961 acgcgtgaaa cattttgtct ctgacagtaa cgactctgct aaagaccaag cattgaagtt
    16021 tcttgaagca ggagataaaa tgaaaaaatg gatcatgcgt caccatcatc aactcttgat
    16081 tacttcagga atgattgaat tattggctct ttaccagcaa caaaaattcc ctggactggg
    16141 agtgttgaaa aagtggtcac ttatgcatca tttggaactt attagtcacc tgctagatca
    16201 tccaaaagat tggcttaaat attattcaaa agttcaggaa ctatatgaag tgatccaatg
    16261 attagataat gtaaatgaaa aaggaataga aaagaggaaa ctggatgtat attcggcaaa
    16321 caaggaaaga agaagtagaa gaagtggtag aaatcattaa tgccgcagcg gatttcattg
    16381 cttcgcaagg ctctcctcaa tggcaaaacg ggcagaaacc aacaaaagaa acgattttga
    16441 acgatattga aagaaaagaa agctatgtat taatagtgga agatcaaatt gctggaacag
    16501 ctgcattagt ctcaggcgtg gatcccgttt acacaaaaat agatggtagt tggaaagaat
    16561 caactggtcc ttatttatcg attcatcgtg tcgctttagc agaaagtact cgaggaaaaa
    16621 aactagcaaa accgttctta gaacatttgt tgacgattgc agccagcaaa aatatccaag
    16681 atatccgtat cgatacttat ccagctaatg aaccgatgga aaagaccatc tattctgctg
    16741 gattcactta tcaagggatg attgaatttc cttttgaaca cggcgaaaga aaagcatatc
    16801 aaataatcat caattgacta taaaatcatc tgagcaaaga taaagacatt aacactactg
    16861 aaaaaaacaa tcatatgata gccaagaggt tgtaacagaa gtggccagct tcaaaaaaca
    16921 agaaagaatt tccgaaaatg cttctcaaat ttttgtggaa ttttagcgta ttttacgaaa
    16981 tagctgcttt ctgaaactcc cttcatcgag aggtcgtgac atagcttttg tcacgatctc
    17041 tcggcttatc ctacgagtat tattcgatac atttatatca ataatccttt ttttatttta
    17101 tctgcagctt cttggaacgc tccatcgttt gacacgacta aaagtgaggg gatagctttc
    17161 aaacgtttca actgtgctaa atcttctaca taaaccgcca gtctggctga aatctgttca
    17221 gatgaatctc cacgtttttt caggcgcgat tgcaatgttt ctttagagat cgtaataaag
    17281 ataggacgga ctaccgtccc gaaatactca tataaatgcc aaaaaccatt tgctgtcaaa
    17341 acggcgtaac aatttcctac tgtcagtttc tttttaattt ctgctttact ggaaccatat
    17401 ttatgttgag catattggtc ccactctgca aactcatttc ccttgatcat ctgattgaat
    17461 gtattgtcag aaacaaaata ataatcctct tgatcttttt ccccaggtct tggtgatcga
    17521 ttcgtgtaag taatgatctt ttgttccggc tgaaaaatag cttctgcgag tgaagttttt
    17581 ccagaaccag aaggaccgat aaatacatag caataattca ttgtcatttc tcctttgatg
    17641 attgaacaag tgaaaaagct ttggaaaata tttccaaagc ttttaaaagt aactttgctt
    17701 ttttgtattt tataccagca agccagtcac aatagcactt aaaaagctga ctaaagtcgc
    17761 tgttagcaaa attttcaatc cgtgttttgc gacaagattt cctttttctt catttaatcc
    17821 cttcatcgca ccagaaatga tcccgataga agagaagttg gcaaatgaaa cgaggaagac
    17881 agatataatt gctgttgtac gctcagtaaa atggaaatta ccatttgcta attctgtcat
    17941 agcaacaaat tcatttgtaa ccagtttagt cgccatgata ctaccggcat ccactgcttc
    18001 tttccatggg attccgctga tgaaggcaag aggggcaaag atgtaaccta ggatctcttg
    18061 gaaagtgatg ccgaatatcc cgtggaagat agcattgatc atggcaatca aagcaacaaa
    18121 accgataagc atagcagcaa ccgtgatcgc cacatggaaa ccatcaagaa tgtattctcc
    18181 caacatttgg aaaaatgtct gttctttgtt ttcttcaatg atcagctcat cttctttttc
    18241 ttctaataca tagggattta caattgaagc cagaatataa ccgccaaata agttcaaaac
    18301 aagggcagtg atcacatatt tagaatcgat cagtgccata taagatccga cgatcgacat
    18361 agaaactgta gacatagccg atacactcat ggtgaacaaa cgtttctcag ataggaaggg
    18421 taattccttt tttatggaaa taaaaacttc cgattgtcct aaaatagcag aagctactcc
    18481 attataagat tccaatttgc ccatcccgtt gattttactt aatcctagac caacagcttt
    18541 cataaataat ggaagaatct tgatatagcg tagaataccg ataatagcag aaataaatac
    18601 aattggcatc aatacggaaa gaaagaaagg tgcacttcct ttatttgcta caccggcaaa
    18661 aacaaagtca acaccttgcc cagcaaaagc aagaagatga tcaaacattt ttgccaatac
    18721 agaaacaacc gctgtaccaa aagttgtacg taataatacg aaaccaagaa caagctgtaa
    18781 aacgaacatc acaattagtg gtttcaattt gattgctttt ttgttactgc tgatcaaaaa
    18841 agcaagccct aatgtgacaa ataaaccaat aatacctaat aaataactca aataatcact
    18901 cctattccaa attccgtttt attttacctg ttttgcaaat aaaatactaa tctttagtga
    18961 aattttcatt acttttttag agtgaaacgc attcattttc cattagaaaa taaagcgaac
    19021 aaaataaaag gtttaaaaag tgtaacgtct tagtaaagtc tataaaaaaa cgaaactatc
    19081 gattaagcta atagtttcgt tttcaacatt tataagtaaa ctttttctat tggtaatata
    19141 gattttcgga agggtagaca ggtgcgcagg taatatcgat tcctagtttt ttcaatgttt
    19201 gttcatcatt tttattgacc atcacagtag agtgggcttg tgctccctca agttcagcta
    19261 gtttatcata agcaacttga gcgattggat tcgttactgc actgattgat aaagcgatca
    19321 agacttcatt gatgcttagt gcatccaaag aggaatgaag tgtctttgat ttcaatcctt
    19381 gtattgtttc aagaactaat ggtgacaata aaggaatgtc atctgcgata tgggctaaat
    19441 gttttactgc atttaagata actgccgctg acgcatccat caatgaactt gtacgcccgg
    19501 taatgatgac ttcatctggt aattcgattg caatcacagc agaaggatct tttgtaggac
    19561 tttgcgcttg aatcttatct gcatagtcat gggcagggac taccacgttt cggtcttcag
    19621 gacgtaaacc gacttcttcc ataatcagtt tgattcgatt aactgcttct tcgtcgatca
    19681 atcctttttt gaagtcgcat tctgtttgga aataacgacg gatgatttct tgtttagagg
    19741 cttcttggac aacggtatca tccacgatcc caaatcctac acgattcaca cccatgtcag
    19801 taggagattg ataaacagat tctttaccag taattttttc aataatacgt ttgattacag
    19861 gaaatgtctc aacatcccga ttatagttca cagctacttt attgtaggca tcaaagtgga
    19921 aggaatcgat catattgaca tcttttagat cgactgtggc tgcttcataa gcaatgttca
    19981 gcggatgttt caacggaaca ttccatactg ggaaagtttc gaacttggag taaccagctg
    20041 catgtccttt ttggctttca tgatacagtt ggttcaagca agtagcgagc tttccacttc
    20101 ctgggccagg agctgtcact acaacgattg gtttagttgt ttcaatatac tcattttttc
    20161 caaaaccatc ttctgagacg attttttcta cgttgactgg atagtcttcg atttctgagt
    20221 gtttatagac cttgatgttt cgtctttcta gtttattgat aaagactttc gtggcaggct
    20281 gtccactata gcgagtgata acgacactgt tgattaataa gccataacca cgtaattcat
    20341 cgattaaccg caagatatcc atatcataag taatcccata atcaccacgg atcttattac
    20401 gttcaatatc tccagcatag acgcaaatca gtatttcagc ttgatctttc aatttttgca
    20461 gcaatttgat tttcgcatct tcttcaaatc ctggaagtac acgtttcgca tgcttgtcgt
    20521 caactaactt cccaccaaat tctaaatata atttgtcata gtggttcact cgttccaaaa
    20581 tatacgctga ctgctcttca atatattttt ctgaatcaaa accatttttt ttcactaggt
    20641 tccctcctta agtactaaag cattttttca tttttttgag gattcgtcaa ctggaagagc
    20701 gaaattcctc aaaaaaacaa agaaaatggt ttaaaccaac ttgtctagat agttattcat
    20761 tggtatgata gtttttgata aggaaggtgc tacatgaaaa aaagacagaa aaagaaaaac
    20821 gcatataaac actatatccg ttcgattttt accggttatg aaagaatgct tgaagatccc
    20881 gaattggaac aattgacgtt tacttatcta aacgaagaaa cgcagctaac tcgtgatgag
    20941 catcagcgca ttcatttcac aacaagagat ctgcctagta aataaaaaat aagataaagg
    21001 tcatcggatt cgattctgtc ctatcagatg gaaagactag ttgatcgaaa aaaaggctgt
    21061 accaaatttt ggtgcagcct gttttatgga gcattgcccg ctctttttta taacaatttt
    21121 ttacagaaaa tggtactatt gaagtattaa taagtgaaaa acagatgagt aagaggaggc
    21181 aagtagtatg aaagagccag atacaaatgc aaattatgag ccagacgaac tagaggagac
    21241 gaacgcggat caggatacag gacaagaatc gtcttctttt tacgatatca aagaaaaagc
    21301 aagcatgaat ctttcccaat tattagaaga cttacaggct tttgaaaaag ctgtccgcga
    21361 agaagatatc cctgagatct accgtttata caatggccga ttgaatgaag aattaaaaag
    21421 aagctcaaat gaaaatcacg agatcgatca attgctgatg cgtaagatcc atgatcgttt
    21481 tttacaggaa tttcctttta tggaacaagt agaaaacatc tcgccaacga tgagctatta
    21541 caaaatcggc acttattacc atgatcggcc cacggtcggt attgatgcaa gtctgccaga
    21601 aatttttgtt ttgcctaaga tcgatgaaga gtgggagaaa tattcccaac cggagtcaac
    21661 ggactatgac aaaaaaatca atgagctaga cgcaaaggtg atcactgctc agacagaaat
    21721 tgaacgttta gatgaacaaa taaaagaaat caatcgacaa atgactgatt tgaacgacaa
    21781 taaaggattc ttgaatcgta aaaaaatcga ggaagaaatt caagaattag agaagaagaa
    21841 gcaagtacta tctaatgaaa aattaggctg gcttccttat attgagacgc ctgaaacaat
    21901 ccagcagcaa aaagaagcat tgaagcaaga agcaagagca gaccagcttc gtgcagccat
    21961 cgtagaaaaa gaacaaaggc aaatcagacg ctatttcggt agcaaagagg gattcggtca
    22021 agcaatccat gaattcttga tgaattatct tggcaatgag aatcctaata atcaatctaa
    22081 tgaaggaggt tccgagtatg aatgaacacg atgaagcaga atggacaaaa aatgaagaac
    22141 aaacagaaga tcatgaagaa acgaacactt cattggaaga acaaacaagt aatcctctgg
    22201 atgacacaga agaaatccct atgctagagg aaacaaaaat acatgaaaaa gaaaaagtaa
    22261 ctccgacgaa tacgaatact gcagaaaaca cccatgctta cgaaataact gaaaatcccg
    22321 ggtatttaga aggaaaagag caagatcatc aagatatcgt cagtttgatt gaaacagcgg
    22381 aagcagaatt agcggattta cgtatgagaa aagatttgat cgaatcagat tttccacaat
    22441 tattagaatc atatgatcgt tttatattga atgaagaacc aatctccacg atcggaaaga
    22501 caagcctgat cgaatacttc acgtatgaat tactgaaacc gttgaatgat attggtctgg
    22561 aacggtcaaa ttcagcttat gacacatgga aaacaagaca tttttcgatc aattatacgt
    22621 tgaatggaca aaacgaatta gtcttttcct ttgtattacc gaccatcgat caacgatacc
    22681 aagtggaatc gatgagtctc ctaaacgtat caccagagac aatggaaatc aatgtccaag
    22741 atgaccgggt gctttctttg atccgacact ggtcagtgga tcgagtattc tctgcagggc
    22801 agatcacgat attcaatcac aaattgaatc aattattagc ccatgcaaga acacttggtt
    22861 ttaccgttaa tcagacatta ctagacaata cgaagccact tcgtttgaat cttcagagcg
    22921 aatttgaatt gacgaatgaa gtattggatg atatttttat cgtggcaatg aaaaatccat
    22981 cctacgatat ggaaaagatc aacgaaacta cttatcaagt acgattagat aaaggacagt
    23041 ctttaacgat aacaacaaac gaaaaagatc aaacagtctt gtcgatctca tctggagact
    23101 acccacgatc tgtcattgat ttctttatca attacgaatt cttggtccca ttaatggtga
    23161 gaaagtcctg attggataag ttggattacc agatgtcatt cagataattc tgttccaaag
    23221 gacttgcggc agttatccag aataattgtc acaagtcttt tctgtatgta ctaaatggac
    23281 gtgaataact tgctgatgaa ggagggattt cattaggaat gtccacaaat aaaatagcac
    23341 agcttagctt attgtctgct gcttgtgtag caggtaggat cgcttttcaa tttattccga
    23401 atttccagcc aatgactgct atttttttat ttatagtact ttacttaagc ttaaaagatg
    23461 cgttaattgt catgagtctt tctattgcga tcagcagttt ttattttggt gttggaccat
    23521 gggtcatcgg ccaatggatc agctatactg ttgttctgag tttgttttca atcgtctgct
    23581 tgagaaaaac agtgcaaagg aatctatggt taaagggagt ctgttttttt ctggcgggca
    23641 tggtttacgg aataggtatg accgtttttg atacgctgtt atatcgtttg cctcaacctt
    23701 ggatctatta ccttcaaggg gtgtcgtttg atttgatgca cagtataggc aatgttgtgt
    23761 tctttcttgt ttttctgcca gtcgtcaaac gtttttacaa tcattcagga ggaaaaaatg
    23821 aaaaaaataa tttatagttt gcttgcaatg agtgtcagta tactcatttt gacaagttgc
    23881 agtacaacct atacacaatc ggcttccaac caaaaggagg aagtaaaaac atcgcaaaaa
    23941 gcaacaatca cgttacaaga aaatggtcaa aacttttcgg aaaaagaagt ggtcttcaaa
    24001 aaaggcgatg atttactgag tattttgaaa cgaaattttg aagtcaaaga agataacggc
    24061 ttcatcactt cgcttgaagg gcactcacag gatgagaaaa atcaactcta ttggatgttt
    24121 actgtggacg ggaaaagtgc tactactggt gccaaagaga tcaaattgaa tgatggacaa
    24181 tcggtcattt tcaatttgtc taaactataa gtttcataaa aaactgttaa agggcgaata
    24241 ttgccataat tcagtgagtt cagttgatga aagaaggaaa ataacatgga aaattttaaa
    24301 aaaagcggaa tgttttccgt aattgctgct ctaggggcta atattctagt tgctatcagc
    24361 aaatttgtcg gctatgcaat cagtggcagt gccgccatgc taaacgaaag catccacagt
    24421 atcgttgatt gcagcaatca gattttgctg ttattcggtg ataaacaagc caataaagga
    24481 cagagcgagt tgcatcaatt tggtgaagga cgagcaaaat actttttcag tacaatcgtc
    24541 gcaatgatgc ttttcttcgg cggtggggca cttggggtga tggaagcttt tgaaaagttg
    24601 acgcatccat cccatgaagt gggtaatccg atcattgtta tagcaatact gttatttggt
    24661 ttggtaatcg aaggaagttc gttacgcgtg gcaatgaaag aaatcaaaga attgaatact
    24721 gaaaaacttt ccacgatgcg ctttttgcgc gaaagtcgac acagtgaaat actgattatt
    24781 tttacggaag acttgtgtgc tgtaatcggt ttggtcttgg ctttagcagg aacaatattg
    24841 acttatgcaa ccgggattgc tgcatttgat gcgatcagcg gattgctgat cggtttcttg
    24901 ctgatgggag ccgccatttt cctagcaaaa gaattttata gtctgatcat cggtgaaagc
    24961 gtgacagcaa gcgatttatc aaaaatcaag ttagcttttg aacgtcctga gatcagtcgg
    25021 ctgatcgata taaaaacagt tcatttaagt ccaacggaga ttttagttgc agcaaaagca
    25081 gatatcgttg gtaacaaaga gaatgaagct tactcggtca tcaatgatat tgaaaaaaat
    25141 atgcgtcagt ctcttcctga caagaagatg tatatataca ttgaaacaga taaatatgac
    25201 ccaaattatt ctcgtaaata aaacgtttat tcatgaattg aagcaaaaat gagaaaaata
    25261 taatcttgtt tttaaataaa acggctacaa tttagcgaat ttatgttaca atgagtccgc
    25321 tgtttatttg ttgtaattaa aggagatatt ttaatgaaca aaaagaaaag tgcgccatgg
    25381 cttcaagctt tgcatgcagt gatgccactt tgtataagct atataccagt aggattagct
    25441 tgcggagtat tgttgcaaaa agttggattt gatcctctct tatcaggact gttatcgatt
    25501 ctggtctttt ccggtggcgc gcagttttta gttgcatcta tgcttacgac gcaagcatca
    25561 tttgcaacga cattattgat ggttttcttt ttagaattac gctatacatt attaggagca
    25621 agtcttgccg gttttatgaa aaaagaaaag cggacgtttt tggctgtctt ttctcaatca
    25681 ctgaatgatg aaaactatgc ggtgaactac ttaaagtttt caactgaccc ttcctggaat
    25741 aaaagaaagg ctttatatgt caattggttc tcaatgactg catgggccgg tagcaatatg
    25801 atcggtaatt ttttcggctc ggtgattcga gtagatgctg accttgtcca ttttgctttg
    25861 acggccatgt tcattttcat gtttatcatg cagatgaaaa gcgcactact gatctttaca
    25921 ggtttattct caggcgtatt aggggtcgta ttcatggtat tattccaaaa tactttcgga
    25981 ttgattgcag caacagtcat tgcttcattt gccggattta tgctggagaa ggcattcaaa
    26041 aaagaaaaag ctaagaaact aagagaccgc ggtaaagatg tcaatgagcc gctcttcaac
    26101 ttcccggaac aggaggccaa tcaccatgac taaactatat ttgctacttt tagttgcttg
    26161 tctgttcatc gtggcatata tcccgcgctt gttcccgatg ctttatttta ctcatcgaaa
    26221 agtgccatcc tggttcagtg actggatgaa atatgtaccg gtggcattat ttgcggcgtt
    26281 agcttttaaa gatgttttca ttactcatga acatctggat attgcttgga atatcaaaat
    26341 agctgcaatg attctagtag caggcgtggc ttacaaaaca cgttcgatgg cgttgtcagt
    26401 tttgacagga cttgcgtctg tctttttgct gtctatgctt taaactgttt tattttatta
    26461 aaaacagaga ttgcgacata agttgttcaa actgatctat ccgaactgca tttgcaacag
    26521 tcaattctgg atgcaagttt attcgtaggg ataaaaaaga ttagtgttgg cttatgatgc
    26581 aagctctttt ttattttagg aattagagag ggatttgtcc ttctatgatt ttttgctata
    26641 atagggaaga tgtttatttt atgaaagaat agttgaggga atttaaatga ataaaaaatt
    26701 gatgcaagga acattttggc taacctttgc taatcttcta tgtaaagttt taggggtagt
    26761 ctatttgatt ccctggctaa gtatgatggg gaacaatcaa gatggtatgc ttgctacgac
    26821 attgtataat gttggctatc ttccttatgg attgttcctg atgcttggta cagtcggatt
    26881 tccaaatgcg attgcaaaaa aagtagctgt agccacaaaa gaaggagata acgctgcttg
    26941 tcggctgatt tttagaagta cgatcaatat catgtttgtc atcggtatcg cctctgctgc
    27001 actgatgtat ttatttgctc ctttactagc cggaatcagt ccgattgcta acgttaataa
    27061 tgggatacta gctatcagaa gtttatgccc atctttgatt gctatcccaa ttcttagtgc
    27121 aatgagagga tatttccaag gaaaaaatga tctgcggcct tatggaactt ctttgattat
    27181 cgaacaggct gtgcgagtga tcgttatctt ggctggtact tattacttgc gcgtattgac
    27241 tgacggaacc atattagaag ctgtattgat cagtacagtc gcttctttct tcggtgggtt
    27301 agctgcgatt atccatatgt atatcgtcgg acgcagaaaa gacttcttcg aacttaaaga
    27361 ttttttgatt tccagccgtt atttcgaaag agaaaaccga tctgcctctg tctctatcat
    27421 tcgggaaaca ctaccgttta tttttgtcgg ctcagtcatt actatcgttc aaatgatcga
    27481 ccaagtaacg atgaaaccac tgcttcactt tttccgtcca gagatcgcag atcagcaact
    27541 ggaattctta ttcagccgtg cttctgtcaa tccaaataaa ttgacattga tcctgatctc
    27601 catggtagga acagtagcta taagcagctt gccaatatta agcacgatga agaaaaaaga
    27661 tcggatacaa atcgaaaaaa cagtaggaga tagtttttcg attgcgttat tgattcttct
    27721 tccttcattg actggaatgt ctttattagc tggaccacta tacacattgt tttttggcta
    27781 tgatcctgaa agtgtcggat atttccaaat ggctttgcta gcttctttgt ttttctcttt
    27841 attcacgatc ctatctacga tgcttcaatc tttgaatcac catcgttttg cgatccgatt
    27901 aactgtagaa gcaattatcc tgaaagtcat cttccaagta atcggattag gattagttgg
    27961 cggatacggg atgagcttat caaatggctt agcattcggt attgtctttg ttcgcggcta
    28021 tcactttatg tgcaaagaat accgaatagc acctttagca aagatcagca attttttctt
    28081 aaaaactttc cgctccaccc tgattatgct tgcagtatgt tttgccgttt tctttttgct
    28141 gaatcagcga ttgtcaatgg aatcaaaaag ctatgcaatg atttattgtg tggctgtagg
    28201 aagtattggc ggcttagtat ttttattttc tcaattcgga aaaaatgggt ttcgaatgtt
    28261 gaaaaatgtt aaacatcata gcagaagcaa agaataggtt aactaatcgt tttttgctgt
    28321 cacttgaaaa acggcaactc gtatgattac gcttgtcgtt tttctatttt ccaattttag
    28381 tttcatatct aatcttactt atgatatagt tataaggtct ggaaaaattc tttgaaacga
    28441 ggaaattaaa cacatgaaac ttgcagtagt tacagatagt tcggcatatc tgccggaacg
    28501 tatcaagaac catcccgact tattcgttat tccaatccct ttgattatgg atgggaaaat
    28561 ttataacgaa ggaatcgata tcgaagcgga tgaatattat ggacttttaa aaaacagtaa
    28621 agattttcct actacttcac aacctgtcgt aggggaagtt ttgggtttat atgaggaatt
    28681 gaaagagaaa gggtatgaca cgattttaag tatccatctt tcttcaggga tttctggctt
    28741 tgtcaacaca ttatttgcga tgaaagatga catcagcgga gtaacggtca ttccttatga
    28801 ctctaagatc actagtatgc cgatgggaca catggtagaa gctgccttag acttaaatga
    28861 tgaaggtcag tcccttgatg aaatacttgc acatatcgat cgaatccgag ataataccta
    28921 tgcttatctg attgtagatg atttgaacaa tttagtacga ggaggacgtt tgacaaatgg
    28981 tgcagcgctg attggcagct tattaaaaat caaaccgatc ctgacatttt ctgaagggaa
    29041 aattgtttta ttcgaaaaaa tccgttcaag taaaaaagca tttgctcgag ctgaagaaat
    29101 tattggtaga agaaataacg aaatacaacc gccagtcaaa ctttacgtga tacatgcaaa
    29161 taatttagaa gtagcgattg cggagaaaga gaaattgcag aaaaaatatc caaatgcaga
    29221 aatcgaaatt ggtcatttcg gaccagtcat tggtacacat ttaggtgaaa aagccatcgg
    29281 cattgccatt tcagcgcaat aattcctttt attcaaaaaa cataccaaaa atat
//
LOCUS       DAZKOR010000030        29162 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000030 DAZKOR010000000
VERSION     DAZKOR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.030"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..996)
                     /locus_tag="ACG8ZM_001220"
     CDS             complement(127..996)
                     /locus_tag="ACG8ZM_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0492191.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(1700..3592)
                     /locus_tag="ACG8ZM_001221"
     CDS             complement(1700..3592)
                     /locus_tag="ACG8ZM_001221"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492192.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(3589..5316)
                     /locus_tag="ACG8ZM_001222"
     CDS             complement(3589..5316)
                     /locus_tag="ACG8ZM_001222"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492193.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(5332..5982)
                     /locus_tag="ACG8ZM_001223"
     CDS             complement(5332..5982)
                     /locus_tag="ACG8ZM_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0492194.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            6186..6482
                     /locus_tag="ACG8ZM_001224"
     CDS             6186..6482
                     /locus_tag="ACG8ZM_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0492195.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            6674..7294
                     /locus_tag="ACG8ZM_001225"
     CDS             6674..7294
                     /locus_tag="ACG8ZM_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0492196.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(7386..8552)
                     /locus_tag="ACG8ZM_001226"
     CDS             complement(7386..8552)
                     /locus_tag="ACG8ZM_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0492197.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(8564..8917)
                     /locus_tag="ACG8ZM_001227"
     CDS             complement(8564..8917)
                     /locus_tag="ACG8ZM_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0492198.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            9045..10067
                     /locus_tag="ACG8ZM_001228"
     CDS             9045..10067
                     /locus_tag="ACG8ZM_001228"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0492199.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            10086..10247
                     /locus_tag="ACG8ZM_001229"
     CDS             10086..10247
                     /locus_tag="ACG8ZM_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492200.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(10506..10940)
                     /locus_tag="ACG8ZM_001230"
     CDS             complement(10506..10940)
                     /locus_tag="ACG8ZM_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492201.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(11050..12909)
                     /locus_tag="ACG8ZM_001231"
     CDS             complement(11050..12909)
                     /locus_tag="ACG8ZM_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0492202.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            12910..13050
                     /locus_tag="ACG8ZM_001232"
     CDS             12910..13050
                     /locus_tag="ACG8ZM_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0492203.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(13027..14187)
                     /locus_tag="ACG8ZM_001233"
     CDS             complement(13027..14187)
                     /locus_tag="ACG8ZM_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0492204.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(14494..15918)
                     /gene="araA"
                     /locus_tag="ACG8ZM_001234"
     CDS             complement(14494..15918)
                     /gene="araA"
                     /locus_tag="ACG8ZM_001234"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0492205.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(15934..16632)
                     /locus_tag="ACG8ZM_001235"
     CDS             complement(15934..16632)
                     /locus_tag="ACG8ZM_001235"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0492206.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(16589..18250)
                     /locus_tag="ACG8ZM_001236"
     CDS             complement(16589..18250)
                     /locus_tag="ACG8ZM_001236"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0492207.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            18468..19868
                     /locus_tag="ACG8ZM_001237"
     CDS             18468..19868
                     /locus_tag="ACG8ZM_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0492208.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(20120..21199)
                     /locus_tag="ACG8ZM_001238"
     CDS             complement(20120..21199)
                     /locus_tag="ACG8ZM_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0492209.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(21285..22244)
                     /locus_tag="ACG8ZM_001239"
     CDS             complement(21285..22244)
                     /locus_tag="ACG8ZM_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0492210.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(22256..23767)
                     /locus_tag="ACG8ZM_001240"
     CDS             complement(22256..23767)
                     /locus_tag="ACG8ZM_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0492211.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(23789..24353)
                     /locus_tag="ACG8ZM_001241"
                     /pseudo
     CDS             complement(23789..24353)
                     /locus_tag="ACG8ZM_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            complement(24359..25222)
                     /locus_tag="ACG8ZM_001242"
     CDS             complement(24359..25222)
                     /locus_tag="ACG8ZM_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0492212.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(25236..26132)
                     /locus_tag="ACG8ZM_001243"
     CDS             complement(25236..26132)
                     /locus_tag="ACG8ZM_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0492213.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(26307..27596)
                     /locus_tag="ACG8ZM_001244"
     CDS             complement(26307..27596)
                     /locus_tag="ACG8ZM_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0492214.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(27568..29085)
                     /locus_tag="ACG8ZM_001245"
     CDS             complement(27568..29085)
                     /locus_tag="ACG8ZM_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0492215.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8511 a         6134 c         5037 g         9480 t
ORIGIN      
        1 aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt tctatctcta
       61 gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc gttaagaaag
      121 aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct cactgacgaa
      181 ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt tgtctaagaa
      241 gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt cagtatcgat
      301 catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa ttgcgcctcc
      361 gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga tgccctcttc
      421 ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt ttgttaagtt
      481 agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac tgaatttttc
      541 ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc tttggtcgtt
      601 cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga gcaaaatccc
      661 catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac ggatacgaca
      721 aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt tcagcttttt
      781 gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca tttcacttcc
      841 agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat caaagccaac
      901 aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg ctacactttt
      961 taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc tctacgtcaa
     1021 gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt tgttaaaaaa
     1081 aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa aactcttata
     1141 aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa acaaaaatag
     1201 ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
     1261 ttaaggaaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa ttttgtgaaa
     1321 atggctttct acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1381 cgtttaccaa gaggttgtga gcctgccgtt taactccgag tcacaaggag caatttcaaa
     1441 aaacagcttc tcaggtttgt tgactattgt gacttgtgac cgaggagttg gcaggagaac
     1501 accgtttaag gaagaggttg taaaatcagc ggtttgacct gagtattaga gatacaaaac
     1561 ggaaaaatag cttctcatat tttgccgttt tttgagctaa taccgaagga cagctgattg
     1621 aacaccgttt attcggaaaa agggactgtg acacgacttt tgtcacagcc cctaaaaaaa
     1681 ataataaatt tgtactattc tatgaagctt cttcttcaga aaactgactg ttataaagat
     1741 ctgcatagaa tccgtctttt tccatcaatg tatcgtggtt tcctgtttct acgatcgaac
     1801 catgattcat cacaataatg ttgtctgcat cgcgtattgt agacaagcgg tgagcaacaa
     1861 cgaaacttgt ccggttttcc aataggcggt tcatcgcttt ttgaatcagt atttctgttc
     1921 gtgtatctac actagatgtt gcttcatcaa ggatcaggac atctggattt gctaagaatg
     1981 cccgcgcaat tgtgattagc tgacgctgcc cttgcgaaat attgcttgca tcctcgttta
     2041 atacagtgtt atatccttct ggcaatttac gaacaaaatc gtctacgtga gcagctttag
     2101 cagcctcaat gatttgttcg tctgttgcat cgtcgttccc atatttgata ttgtcataga
     2161 tacttcctgt aaacagccaa gtatcttgca gtaccattga aaactgtgcg cgcaattcat
     2221 cacgtgaaag atcacgggta tctacaccat cgtaacgaat actacctcca gaaacatcgt
     2281 agaaccgttc aagaaggttg atcagtgtcg tcttccctgc accagtcgga cctacaatag
     2341 cgaccatctc accaggtttg acatccaagt taaaatcagt cattagcaag ttatctggtg
     2401 tgtagccgaa ttgaacattt tcaaaacgaa ctttgtatgg tgtatctttt tcttctggaa
     2461 ggccagattt ttcattggac atctcttctt catccaacac ttcaaatacc cgttcggctg
     2521 aagcaactgt tgattggata gtgttcatca aacttgcaag ttgagtgatt ggttgagaga
     2581 actgtgttgt atattgcaag aacgcttgaa catcccctaa tgtcatgttc ccatttgcaa
     2641 cttttactcc gcctagtaca gcgacgaaca catagcctag atttttaacg aaattcatca
     2701 acggcatgat gattgccgaa ataaattgag ctttccaacc agcggcatac agattctgat
     2761 tttctttttc aaatacttcc tggtcttttt cagtatgatt gaatgtttta acaatggtat
     2821 gaccaccata tgtttcttct acttggttat tcaataaacc taaacttttt tgctgatctg
     2881 cgaaaaacac ttgtgatttc ggcgctacga tcatcacgat gatcaagctt aaagggatca
     2941 tcaataacgc gactaacgtc aattgccagc tgattgaaag catcatcacg atgaccccaa
     3001 tcaatgtgac tgcactagtt acaaattgag tcaaattttg ctgcagcgtg cttgcgatgt
     3061 tatccatatc attgattgct cgagacatga tatccccatt tgtatgtgta tcataatagg
     3121 aaatcggtac acgattcatt ttggcttcca aatcatgacg taattcataa actgttcgtt
     3181 gggaaacacg tgtcataata aattgctgta aaaagttgaa tacagcagaa atcaaataca
     3241 tcacgattac tgtcgcaatg atctgcgcga ttttatcaaa gtctatcgga aattttgtta
     3301 cttgttggcc ttgttgcatc tgttgagcgc cagccattac acctttgaat atttctgtgg
     3361 ttgctttccc caagaccttt ggtgtttgga tttggaagat cacggctgct attgctagta
     3421 caaatacagc gatgatcgca accatacgtt tagacatata gccaaacaat cggcgtaccg
     3481 ttccccagaa atttttaggc ttttcacctt ttacaccgag atttctgcca ggtccaccat
     3541 gtcctggacc gcctgctggt ccatgagagg gacgtgtgtt tgttttactc atgctaagtc
     3601 ctcctctctt agttgtgaat gaacgatttc ttgataagtt tcgtttgttg ctaatagttc
     3661 ttcatgtgtt cctttgccaa ccagttttcc ttcatctagc acaaggatca tatctgcatc
     3721 cattactgta cttactcgtt gagcaactaa aacggtgata ctttctttca tattcgtttt
     3781 caatgcttgg cgaagattgg catccgtttt gaaatccaac gctgagaagg aatcatcaaa
     3841 cacatagata tctgctgttt tgactaatgc ccgtgcaatt gccagacgct gtctttgtcc
     3901 accagagaag tttcctcctc cttgttccac atggctatct aagccatctt ccatctctga
     3961 aacaaaatct ttagcttgtg cgatttctaa tgctttccaa atcatttcat ctgttgcatc
     4021 cggcttacca tactgcatat tgtctcggat cgttccagag aacagaactg ctttttgagg
     4081 aacaaagccc attaattctc gcaaattata ttgagtcatg tcactaatgt ccgttccatt
     4141 tatttggatc gaaccagatt cgatgtcata taatcgaggg atcaaattga ctaaggtcgt
     4201 tttacctgaa ccagtccccc ctatgattgc gacgatctcg cccgacttgg cttggaaatc
     4261 aatatcttcc aatgcaagtt tttctgctcc atgatatcga taattgacat gattgaaggc
     4321 caatgttgcg ttttttccag caaaagaaac tgttttagga ttttcagggt catggatacc
     4381 gattttttca tccaacactt cattaatacg gtcagctgaa gcttgtgcac gaggcaccat
     4441 gataaaaata gcggaaagca tcataaagct gatcaagatc tgcatcgcat aagtcataaa
     4501 tgcaatcaga ttaccgactt ccaaagtcat atctgctatg tagtgtccac caaaccaagt
     4561 gattgctacg tttgtaccgc tcatgatcaa tgtcatcatt ggcatcatca atgcgacgat
     4621 cgtattaact ttgattgccg tatttgtata atctttattc gctaaatcaa aacgattttc
     4681 ttcaaagcgt gtcttatcga atgctcgaat cactctgacc cctgtcaatc cttctcgaaa
     4741 taccaggttt aaacggtcag ttttcttttg catacttttg aataatggaa cagcaaagta
     4801 catgattccg ccgatcaaaa taatcattac tgggatgaca actaagaaga tttttgtcaa
     4861 ctgatgatct ttttgataag caagtatgct cgcgccaatc aacgtgattg gtgcattgat
     4921 catcatacgt aaaaacattt gtgtaaccat ttggatttga gtcacatcat tggtcgttct
     4981 tgtgatcaaa gaggccgttc catatttatc aaacgcatct tttgtcagac tctctgattt
     5041 tttatagaca tccgtacgca attgcttacc cagtttctgt gattcacgag tagcaaaaaa
     5101 tgtatttgcg atagcagcta agatactgat caaagaaaag ccgatcatca caaaacctgt
     5161 atgccaaata taatcgacat ctccttgcgc taccccttta tcaataatat tggaggtaag
     5221 tgtcggcaaa tataaatctg ccagtacttg gatcaccatg aaaactgcag ctgcaattgc
     5281 tgaagtcaag gagatccgtt ttacgatttt tagcatctag ttcccttctt tctatttata
     5341 tactccgttt ttcagccaag aaagtttcaa attgaattca ctgattgctt gctctactga
     5401 gtaatcttca gatgttgaaa ataagcgata cgaatgcgca attgttgtaa atacagtatt
     5461 catcactaat tggatcagta attccaattc atgatcattt tttactttca gcttcgtccg
     5521 atcgatcaat tcaagcaact cttttgcatc ttgtttgtgt tctttatgtg gatcgtgagg
     5581 cgttttttgg cgactcgtat gaggagcgac ttttcttgag gcacggtaat ccatgttcat
     5641 aaacaagtta cgataaaaag aagcattctc gccttccaaa atctcataaa tcattttaga
     5701 aaaataggat tcgaatcctt tgaatagatc accatcggat tctttcatta gttgtaacat
     5761 atctttagag ctgtcacgtc tcattgtctc aaagtaataa aaataaagat cctctttatc
     5821 ttcaaaatac tgatagaaac tgcctctagg gatatccgct aatttgacga tattagcaat
     5881 cgaagcttct tttaagggta ctctagaaaa ctcgattctc gcagcctcga ttagtctttt
     5941 ttgtttttct ttaggcaaat ggaaaaatgt ctgtgtcggc atatggattc tcctttcttt
     6001 caagatgaca cattgtcata tgacgctatg tcattataac acctcctacc tgttttgcaa
     6061 gacatttgca tagttttttt cattatcatc cgatacatta aaaaagtgat aagaggaatc
     6121 cttccatttg aattaatgag agttgcccat taaaatagcc acatactaaa caaaggatga
     6181 gataaatggc ccaatcaatt agtgtccaag acttcaataa attgcctctc gatgctgaca
     6241 gcatcgtttt agatacaaga aataccttag attacacaaa aaaccactta agccactcgc
     6301 tttctatccc cgctgctatc ttgccaagac aattaaaaaa tctagatcca aagaaacttt
     6361 attatattct aagctataca ggcaggcgat cagaagcatt agccgatcaa cttactgcta
     6421 atggttttcg tgctgtttat atcattggtg ggatggaagc tcttcagtcg agtgttgctt
     6481 gaatattgct tgttagattc aaaagaggct gtgacaaaaa atcgtgtcac agcctctttt
     6541 ataccctgtc tttttttagt tatttattct tcaaccttat caatatgact tgatagcctt
     6601 ttctttttcg agtaaactga ttttgttagt catagttata acaatcatag caataaaagg
     6661 agaaggtaag catatggcat ctgttttagt agtcaaaggt catcccctaa ctgcagaaga
     6721 atcgcgcaca gttaaggcat tgacgtcttt tttaacaagt tataaagaaa atcatccaga
     6781 tgatgaagtg actgttttgg aactttatcg cgatgatatt ccggaaatcg atgaagaatt
     6841 attatcaggt tgggaagcat tacgtgctgg tgcagaattc acttccttat cagaaagtca
     6901 acaacaaaaa attgctcgtt tcaatgaatt gactgatcag ttccttgctg ctgataaaat
     6961 cgttatcgca aacgccttat ggaatctaaa tatcccaaca aaattaaaag cttggttcga
     7021 taccgtcaat gtagctggaa aaacgttccg ctatacagaa aacgggccag aaggattagt
     7081 gacaggtaaa aaagcactgc acatccaatc taatggcggt gtatacaacg gacaagactt
     7141 tgcctctcaa tatgtaaaag gtattttaaa ctttgtaggc atcgatcaag tagatcagct
     7201 attcatcgaa ggaatcgatt atgaccctga ccgtgcagat gagttgatgc aaaatgcatt
     7261 agataaagct gctgctttag gtaaatcatt ttaaaaattg agataaatgc atacaaaaga
     7321 gacagaggaa aaaatcttca aagcagcatt tgtgccttgg atattttccc atctgtctct
     7381 ttttgttatt ctttctgctt tttcaacgga ttttcttcat aaagagaaga gattgttgtg
     7441 taccattcaa aaacatgagt aatcactact ttagctgctg cataaacagg aatccccaag
     7501 atcacaccga tgattccgaa caatttccca gaagtcagca gaactaataa aatcgttact
     7561 ggatggattg ctaactgatt ccctagtact aatggagaga tcaagcgtcc ttctattgtc
     7621 tgctctactg caaaaacgat cagtactttg atgatcatga atggacctgc tacaatggct
     7681 aaaaagatag ccggaatcat cgctaaaaat gatcctaagt aagggatcag gtttaaaaaa
     7741 cccgcaataa tccccaacgt gacagcatac tctaaaccaa tgaccgcaaa accaatcata
     7801 aacatgattg ccacagcaaa agccacagtt agttgtcctc ttatataaga agaaacttga
     7861 ctattcattt cactcagcac attcaacgtc ggtttttgca tgcgtgtcgg taaaaatttg
     7921 acaaaataag gtgctaattg tttaccgtct ttcaatagat aaaataagat aaacggcatc
     7981 gtcagcagag ccactaaaat agtcgctacc gcgccgacaa aacttccaag actctgaact
     8041 gtactcttag atatatcttg gatcatatcg cctgccgaat tcatgaactt atcacctgct
     8101 gtttccaact gttcgcgaaa ttggttgaac agcgggtccc gcaaaatctc ggttaattta
     8161 ttatcaatcg tatctacata ttgagggaag ttactgatga agctcactgt ttgttcctga
     8221 atcttaggga tgatcactac gctcccccat gcgattagtg caacaaccaa aacaaacagc
     8281 ccaatgatac tgtaaagcct tggtactttt cgcttttcaa agtaatcaac taccggattc
     8341 atcaagtaat acaagattcc tgctaaaatg ataggcagac ctacgattgc taaaaattgc
     8401 cagattggtg caaacaaata tgaaactttt gtaaataaaa agacgatcag caatattaac
     8461 aatacaacaa gtaaggctgt gactacttga ttatttaaaa accagcgcca aaaccacgat
     8521 accggtctgt tcttcttatt ttttccttcc ataattcctc ctgttagtta taaaaaattt
     8581 ggcttcccaa accaatagca ggcagtctat gcggatgaag atagattcca tttgcaattt
     8641 gtccttcttc tggacaagca tcaaaaatca atgatacgtg agctactgtc tttaaattcc
     8701 cattggccat cggaccgaca taatcgatcg tatatacctg atcatcaaaa gcaattgttc
     8761 cgcctgcttt caaagaaaaa ggttcgtctt ttgatacttc ttgaatcacc gaatattttc
     8821 gtaaggcagc tgttgctgtt ttatcgaaca aaatcagcat gggttcttcc ttgtttattg
     8881 cttctgtccc aatttctgta atctttgcct gaatcattgc actctctcct ttaattgctt
     8941 tcaaaaacag tataacacat tctcattctt tagccgtgtg gaataattat gtgatagaat
     9001 aaaaacgaaa actatttcat tacaattagg aggaatcagc tcatatgatc gaaaagataa
     9061 ttttaggaac ctatacgcgt cgcgaaagta aaggaatcta tacgatcgac cttgatacag
     9121 aaaaagaaga attagccaac ctttcattag ctgttgaaga aaatagccct acctatgtag
     9181 caaaaagtaa agctggcaat ctttacacag ttactagtgt ggatggatta ggtggtgccg
     9241 gtgcgtacga taaggatttt cattttttga atgcggtaac agagtctggc gctcctttgt
     9301 gttatgtagc ggttgatgaa gcacgtcaat tactatacgg ggcgaactat cataaaggtg
     9361 aagtaaatgt ctaccatata ttaggagatg gcgaactgaa agcagcagat tcaatattcc
     9421 atcaagaagc gacaggtcct cataaaaacc aagatcatgc gcatgtccac tatacggatc
     9481 tgactcctga ccaacgatta gtcgtatgtg atcttggtac agatcgtgtg tatacttatg
     9541 atgtgtccga aaacggcaaa ttgaatcttg taactacctt cactgcagag ccaggaacag
     9601 gccctcgcca tcttgtcttt catccaaatg gacaattcgc ttatttattt ggtgaattag
     9661 acagcacagt ccgcgtgctt tcttataata aagaaacagg tgcttttgaa gaattacaga
     9721 aaatatcgac cttgccagaa gaattcgacg gtgaaaatgg aggggcagct atccgcattt
     9781 caaatgatgg caagttcctt tatgcttcta accgaggaca taactcgatt gctgtattcg
     9841 cagtttttga agatggttcc cacattcaaa acaagcaaat catctcaaca gaaggggatt
     9901 ttccccgcga tttcgcacta gatccaagca ataattttgt cgtgtgtgcc aatcagaaca
     9961 ccgataatct gacattatat cgccgtcatc ctgaaacagg attactggaa ttactgaaaa
    10021 aagatgtcta tgtaccagaa tgtgtctgtg tttattttga tcgttagtaa gaaaaggaga
    10081 atgaaatgaa tcagcaagac gtattagaca gactacggga agaactaaat attcctttct
    10141 ttgatgggaa aatcgaagat aaagagtata gcgaagagga atatcagaaa ctcaaagccg
    10201 atttacagaa ctactttgaa caatatgtac agaatgtaga aaactaatca gctttccata
    10261 ctatctgaaa agaacatcag gacaagactt ttccgtctat cctgatgttc ttttctacaa
    10321 ataaaaaacc gccgagatgg ttagactcga cggaaaagga gtatttactc gattttgagt
    10381 aagtacaagg ttgttgttgg ttataggtat gtcattatca taaaggattt tgatcactct
    10441 atcataaagg taatattaca tttatattag ataactctag tttttattgt tatcaaagat
    10501 ttcttttatt ttttcaacaa caaattagtc attttttgga tggcagctgt caattggtct
    10561 gtcgcttggt cttgttttgc ttcagatgta ttgaatagat cttcatctac ccctgattca
    10621 agatcgatac tttggatcat ttcttcacag cctgctacat atgaaagata agctttttca
    10681 aaatttttat gaatgcccat tgcttttgct ggtggacgca aagtactgac tttcataagc
    10741 attgcccgat atttttttgt tccttcttca aataacagga tgatctcttc aagagtcgat
    10801 ttgtccagtt cacttgtttt tccttcatca attgcgtttc tgatcacttc atatttcgga
    10861 tgcattcctt ctccgattgc ttctgtttcc ttgacgattt gatcgatgga ctgcacataa
    10921 tagccaatat tcggtaacat gttcgttttc ctactttctc cattgttgtt attatttatt
    10981 ctacttgttt ttatcctcaa atgacaagta cttgaaaaaa tttaatggtt tctatacagc
    11041 tcgccattct tattcgtgct caaagaaacc atctgctaaa ctatattgtt tccctgcgac
    11101 aatgtcatat tggatcaatt gatattcgtc gtaaatctct tgttgcgagc gattgaattg
    11161 cgcttcttta taccactgtc cgttttgata gtacagttct cgttcaaacg ccggaagttc
    11221 ctgttctaat gataaaagca gctgataaaa gccatttgtt gtctgattcg tctgttccat
    11281 tagattcgct gcaaaataaa acggactggt gattgcatcg tgagtcgttt gcttttctaa
    11341 ttctccttta ctgtcgaaca taagaaattg tgtttcatgc aatgtttgtt tgtcattttt
    11401 tgcttggatc gtatctgaat agatccctgg aagatgatcc ccccaaaaaa caactaaagt
    11461 ccttctagaa agattcttca attcttccgt gaactgcttc agtgccgtgc tgctatagga
    11521 aatatcttgc agataattct ctaagtcaag tgtcccactt ccatctgaag tttttgcagg
    11581 atagtctaga ttatcatact ttccatcgta gggcatatgt gtctgcatcg tgactaaatg
    11641 aatgaactga ggtgtttttt cttccttgag aagtgtcatt acttctttgt aagctgactc
    11701 atcagaaata tatggattat tctctatcgt atccgtatag gtcatcgtac gctcgctgat
    11761 gaactggtca aatcctaatg tctgataaac atcctctctt ttatacatcg aagtattgta
    11821 tggatggatc gctgtcgtct ggtatgattg tgcgtttaac gctgaaacga tagaaggaag
    11881 ctgatccatt tttggtacaa gcatcgtgta aggggtggtg agttgcgcat taaataacgc
    11941 catagaaaaa ccagtcaatg cttcaaattc gatatttgct gttccaccgc catagttttg
    12001 agaaagcata ttgccactat atgtttgatc agctacttca taataatctg ccaatggctc
    12061 accagatact tctacgccgt ttaatcgact aggatctgag aaactttcac tcatcacgaa
    12121 cacgatgttc ggcgactctt cctctacagt tttatttttt tcatcggcta gcttctggta
    12181 tttttcagtg atttctttga tcttttcttt cgaatatcct tcaggttcat ccattggttc
    12241 taccttcaga ttgtacaaaa acccaccgat aaaaccagtg ttatagtagt tcattttctg
    12301 gctgtaaggt atccatagtg ccgttttatt atatgctttt cgcaacaaat tatcgggatt
    12361 gttgaaatta ctgatatacc ctagcagtcc aattgccaca agcagtgtcg ttaatcggat
    12421 caattggaat cgccttcctt ttttcaggct tcgaaacatg taccagcaaa acaatcctat
    12481 cacggttcct gcggcagcta agatcactgt aaacagcata gtgccggtca tatctttcag
    12541 caatccgatt tcagtgatca tcttcaaatc gtctggataa atcggttctt gccgataact
    12601 cattttcata taatttgcaa aacctaatac accgatggct acgacatacg taagggaacc
    12661 taaagggaca gatcctgcta gtgcaatcag aaaaataaga ataatcagca agacgagaca
    12721 ggctaagaag aatttttccg tatgccaaga aaaagcaaat ttcaacgcaa ggtctacgga
    12781 tagatcattt tgacaccact gcaaaaataa attactaaaa acaacaagaa aaaagatact
    12841 gatgattgct gctgttcctt ttaaaatctt tttctgtttg gtcgaaagtc tgcttatata
    12901 tcttttcaaa tgcttgctcc ttcatttcta tcaataaaag aatgttgctt ctcttatttt
    12961 agttgtacga aattcgaaag taaagaacgg aaagaagaat taatgaaatc caaatattct
    13021 ctttctttat ggcctttctg gtttctttaa taagcggacc tttcgtactc cagaaatttt
    13081 ttctagctgt tgtttcactt gattgagctg ttcaaaatca gtactgtcta tgtcaatcaa
    13141 tatgtagaca tgctcattct tatggttacg cgatatatct gcgatcgcta gctcgttctc
    13201 attcagcaga gaaaatatct ctgctaatat attggttttc ttttgataaa atatagtgaa
    13261 tcgaaacggc gcttggaaaa acattcgtgc ggatggataa ttgaccgctt ctctaaccgt
    13321 accgaacaaa agataatctt ttaaggcttc aactgccaaa cgtccgctgt ctgccaaagc
    13381 ttcctgagtc gatcccccta gatgaggcaa cataagtata cggtcatttt ctaagaactc
    13441 ttcttctgga aaatcagtca agtatttagc cagatgttgt ttttccaatg cttgcataac
    13501 agcatattta tcaacaattt cacttcttcc tacattgata agtacagcat cttttttcat
    13561 tttttctata ttcgcctgat cgatcaatcc ttttgtatct tctgtcaacg gcagcatgac
    13621 tacgatgtaa tccgaggtag aaagaactgt ttctaaatct gcttgccgaa cataatccaa
    13681 ctgggcatcg cggatagaat aacccaagac atccatccct aagctatagc aacttagcgc
    13741 tacttttgtg cctatcgctc caagaccaag aagaccgatg gttttgcctt gcaattctcg
    13801 tcccacataa gcacttcttt tgttttctgc ctgttctaaa atattaggac ctgttagtgt
    13861 ttgtaccata cgacttgctt cgattatcgg acgggaagat aagagcaaac agcacaaaac
    13921 aagctctttt actgcattag catttacacc tggtgtattc atgacgatcg tcccgttttc
    13981 agaagctttt tcaacgttga tcgtgttcac cccaacacca gctcttgaaa ttgctagcaa
    14041 atctggtgta agccattcat ctttcacttt cgtactacga ataactattc cttcggctgg
    14101 caaatcatct attattttga aatattcccc ttgtaacttt tctatctctt gttctggaat
    14161 agcatcaatc aaatgaacgt gatacataaa accccctcct tactaacaag tatctcgaaa
    14221 tttctgcaat tagacaactg tttaccattt gacttcctag aagcaaaaag agcagttaga
    14281 gcagagtagg ctgagtgata aaaataaggc tatgacaagt tactgccata agccttattt
    14341 tcgagagcaa aatattgagg gtgagatatc ttgataaatg gtgttccgtt agctgacatt
    14401 gataataaga ctaaatttca aaaaagttga aaagcaaatt tcgtcaatct cttcttattt
    14461 cttaaaacta agtggctttt tcacaacctc tttttatcta ataaccactg tttccaactc
    14521 aactaatttt gcccacgttt caatttgatc tgttgttaga tttagcgaga caacggtatg
    14581 atgaccgcca cctgcctgaa tccatgaatg gacaccttca tggaaattcg gtttagtttt
    14641 ccataacaca cgtgcaacag gtaaattagg tgcttcagtc gttggttcaa aggcttctac
    14701 ttcattgatc agcagtttat aatgcgtacc gaaatctgcc atcgatacga ctaccccttc
    14761 tcctgctttc ccatcaaata caagacgcgc cggatcttct ctatctccca ttgataaagg
    14821 agaaacaacg attcttggtt tcgtcgcagc aagcgtcgga tctacttcca tcatatggga
    14881 ttccaagatt gcttcttggc cactagctag ctcgtaagta taatcttcca tgaatcctgt
    14941 atctttccct cgagccatga ttttcagcaa ccgatcaatc gctgcagttt tccagtcgcc
    15001 ttccccagca aaaccgtatc cttctgccat caaacgttgt acagctagtc caggtagttg
    15061 tttcattcca tacaggtctt caaaattagt cgtaaaagcg ttatagcctc cttcttctaa
    15121 gaaacggcga ataccgattt cctgctgagc ttgtactttt acatgttcct cccatgtctt
    15181 ttcttcataa tcaccatagt cgaaggtata caattccttg tactcttcaa ataattgatc
    15241 gacttcttcg tctgatacgc gatcgattac ttgtacaaga tctccgatac caaaataatc
    15301 aacagtccaa ccaaattgaa tctgtgcttc cactttgtct ccttctgtga cacccacgtt
    15361 tctcatgttg tcaccaaaac gagccacttt tattccaaaa ctttcattat aggctactgc
    15421 aacatccatc caatcagcaa tctctttttg gatctcaggt cgtttccagt agccaacgac
    15481 gattttattt tgtttattta atcgagcatt gatgaaccca tattcgcggt caccatgagc
    15541 ggattgattt aaattcatga aatccatatc gatcgttttc caaggaatgc tttcgttata
    15601 ttgcgtagct aaatgaagca atggtttttg tagaagcttt gtcccgcgga tccacatttt
    15661 tgctggtgag aaagtatgca tccaagtgat cacacctgct acttcttctc gataattgac
    15721 ctctttcatg atttttgtga tcgtatcagc agtaactgct aattcttgaa ggcggattgg
    15781 ataaggcaat tggccatttt cattcagttc atcgaccatt tcttgtgcat gtttctttac
    15841 ttctcgcagt gcttcttccc catataaatg ctgtgatcct acaacaaacc aaaattcttt
    15901 ttctccaata ttcaacataa ttagccactc cttttatttg ttttgtccgt aatacgcatt
    15961 ttttccgtgt tttcgtaaat aatgtttatc taaaatccgt tgaggcagct cttctgaaaa
    16021 tgaattgagc tgtctggtaa ataaattcat cttgcatact tcatctagta cgactgcgtt
    16081 cattaccgca ctttgcgcat ctttccccca agtaaatgga ccgtgtccgt gtaaaagtac
    16141 ccctggaatt gctaagggat caattttgcg ttctttgaat gtttctatga taacttttcc
    16201 tgtttcatat tcgtacccgc tgtcgatttc ctgttgtgtc aaaaatcgag cacaaggtac
    16261 cgatccataa aaagtatctg catgcgtcgt ccccatcgca ggaacatcca atcctgcttg
    16321 tgcccagatc gtcgcccatg tcgagtgagt atggacgatt cctccgattt gaggaaatga
    16381 acgatataaa acagcatgag taggcgtatc cgaagaggga ttgagtttac cttcaatgat
    16441 atgattgtcc aagtccacaa cgaccatgtc gtctgctgtc atctcttcat aactgactcc
    16501 gctaggtttg ataacgaaat atcgtgtttc aggatcaaaa gcgctcacat ttccccatgt
    16561 atatttgacg agtccgtgtt taggcaagtc aagatttgct tggtatactt cttctttcag
    16621 ttgttccagc attttctatc actccttttt cttgcaattg atgaatcgct gttttttcaa
    16681 tgtcgatccc ctcacggtaa cgtctcataa atatctgata gcctgctatt tcttctgctg
    16741 aaggtgtgat ggtgattcct tggtcttgtc caaacacgtc ttctgccaag aagttttcta
    16801 aagacttccc atgtcgatcc atcatatagg ctgctaataa cgcgattccc cacgctccac
    16861 cttctccggc tgtgtccatc actgtaactg gtgcctccat agcagatgct agaatccgct
    16921 gtccaacttc tggtgtttta aaaatccccc catgccccac taaacgatca atttggacat
    16981 tctcagattt caatatatcc attccgattc tcattgcacc aaaagcactg gctaaatgga
    17041 tccgcatgaa attcgctaaa tcaaaacggc tacctggtgt acgtacaaat aatggccttc
    17101 cttcatttac tcctgtaatg ttttcaccgg aaaaataacc ataagaaagt aatcccccgc
    17161 aatccgtatc gccttctagc gctttgttga ataataccgt gaataattta tctctgctta
    17221 tctccatacc caaagaattg gtaaattctt caaaaatact gacccaagca ttgatatccg
    17281 aagagcagtt attcgtatga accatgccta ctagttctcc tgaaggtgta gtgaccatgt
    17341 cgatttccgg atggacattt tctagttctt tttctaatac gatcatagcg aaggcggaag
    17401 taccagccga aatgttccct gtccctttgg ctacactgtt cgtagcgacc ataccggttc
    17461 ctgcatcccc ttctggcgga caaaccggga ttcctggtct caaatttccc gtagggtcga
    17521 tcagataagc ccctgattcg cttaacttgc cagcttcttc tcctgctact agaactttag
    17581 gcaaaatgtg atccaagtca atcgctacgt gcgctttttc gattaactga ccaaaaatat
    17641 ttttcatttc ttgatggtag gttttcgttg aaggatcgat aggaaacatg cctgatgcat
    17701 ctcctacgcc tagtactttc tcaccagtaa gctgccaatg gacataacct gcaagcgttg
    17761 tcaaaaaatt aatttctttc acatgttctt ctttattcaa aatagattga tacaaatgag
    17821 cgatactcca tctctgcgga atattgtatt gaaatttttc cgttaatatt cgttctgctt
    17881 cttcagtgat tgtatttcgc caagtacgaa atggtactag taactgttcc ttgctatcaa
    17941 atgctagata accgtgcatc attgcactga acccaatcga cccgattgtc gtaagattct
    18001 cgccatagat ttcgaatact tctgctgcca tttttcggta actgacgcgc agtcctttcc
    18061 aaatatcttc taatgaatag gtccagatac cattttctag tttgttttcc cactcataac
    18121 taccagcggc aataggagta aaagatgaat caatcaatac agctttgatc ctagttgatc
    18181 caaactcaat ccctaaagat gtacgaccat ctatgacatc taacatactt tgcgcattta
    18241 cttccttcat aatgatttaa ctccctcctt ctcgtcaaac ctgatcaaac cgtcttttta
    18301 gtagcgctta caatgattat tgtatacttt tgtacgtaca tgtcaagcag ttatttacaa
    18361 gaatacaata aaaaacactt gttttgtacg tacataagtt ataaaatgaa attgcttaca
    18421 aaatctgatc aaccgccttc tcgaaattct agaaggagag aatacttatg aaaaaagaaa
    18481 aaaaagtttc ctcatcgttt atttattttt ttggcgcatt tggcggcatt ctatttggat
    18541 atgatattgg tgttatgacc ggcgccttac cttttttaca gcatgattgg ggtttggcag
    18601 gtaaagcttc tttgatagga tggatcacgt cttcggtcat gttaggtgcg atccttggcg
    18661 gctctttatc tggtcttctt tctgataagc ttggacgacg aaaaatgatt cttctttctg
    18721 cacttatttt tatggctggt tctgtccttt ctgcatctgc ccctcacaat ggttcttatt
    18781 ttctgattgc agcaaggatt cttctaggtt tagctgttgg cgctgcatct gccctagttc
    18841 ctgcttatat gtctgaaatg gctcctgcta gactaagggg aagattgtca gggatcaatc
    18901 aagtgatgat tgcttctggc atgctccttt cctatgttgc agattatttg ctaaaagggt
    18961 tgcctgaaac gatggcttgg cgcgtgatgt tagggctcgc tgctgtcccc gcgctgattt
    19021 tatttttcgg tgttttagct ctaccagagt caccgcgttt tcttatgcaa tctggccgct
    19081 tggaagaggc aaaaagagta ttgaactata ttcggacacc aaaagaagct gaacaggaat
    19141 tcgaacaaat ccagctgaat gtcaaacaag aaaagacaac cggaacttct tggcatactt
    19201 tgtttttaga aaaatatcgt tccttagtca ttgccggtat tggtgtagca gtattccagc
    19261 aatttcaagg agctaatgct attttttact atattccgct gatcgtagaa aaagcgactg
    19321 ggcatgcagc gagcgatgca ttgatgtggc cgatcatcca aggaatcatt ttagttgctg
    19381 gttctttgct ctttttggtt attgcagaca agttcaatcg gcgaacttta ttgaaaatag
    19441 gcggtagcgt gatgggactt tctttcatcc taccagctgt tttaggaact gtactggatg
    19501 ctcatacaaa ttcgttgttg attctcttgt ttttatgtat ttatgtcgca ttttattctt
    19561 gcacatgggc tccactgaca tgggtaatcg taggcgaaat attcccacta gctgtccgcg
    19621 ggcgagcatc tggcctagcc tcttctttta attggattgg ttctttccta gttggcttgc
    19681 tttttcctgt gatgactgct tctatgtctc aagaaatcgt tttcggcatt tttgggatca
    19741 tctgcttttt aggggtttta tttattcaag agatcgtacc agaaactcgt gggaaatctt
    19801 tagaggaaat cgaacaaagt gcatctaaaa aaacttatcc taaacgtatt tctatccatc
    19861 attcttaaag aggttgtgac agaagtggtc agcttcaaga aataagaagg aaccgcttag
    19921 tttccagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgatga atattgtgac
    19981 ttgtgaccag ttggtaggag aacaccgttt agtggagagg tggtgaaaga aacgttcagc
    20041 cttgagcgat gacaaaagct ctcacaaaca ttgcgacgag taaccgtagg agcaataaga
    20101 ggcggtaaaa acccgtctct tatttttcgt tttctttcac tgtttcccgt tcaactagta
    20161 caggttcata gagaatattt ggctgtgcct gtcctttttc aatcgttgtg acgatccacc
    20221 gtgcggcatc ttgtcccaac ttttcttttg gatgcgtcaa tgttgtcaat tttttattcc
    20281 ctaaatggct taaagaagaa tcgtctgttc ctacgatcga taggtcatct ggaatatgat
    20341 agccattttc tgtcaactta tctagcatta aactagctat ttgatcgtta tagcaaatga
    20401 tgccatctaa agcttgttct tctttcaacc gttgtactgt tttttcagcg attgtttctc
    20461 gtgtttccgt ggtgaaagta atgatatctt ccgtcaaaaa acgcacatta tactgttcac
    20521 acgcgctgat aaaacctttc atcctgtatt ttccttgaag atcgtcgatt tttgtgatga
    20581 acaacagacg ctgatgtcct ttttttatca aatattctgc tgccataaaa ccgactttca
    20641 catcatctaa acaaatataa gaggcattta actcttcata agcagcattg atcatcacca
    20701 tcggaatgcc ttgttctctc aaagcaacat aaagtgctaa gtttgggtta tactgattac
    20761 ttttcgtagg ctcaacgatc aaaccatcta ccccataagc aatcattttc ttcaaacact
    20821 ctttctccaa ttcgtgatcg ttattcgtac tggctaaaag caatgaatac ccagattctc
    20881 gtaattcatt ttcgattcct cggataatcg acggaaaaat ataatcagag agatatgtgg
    20941 tgatgacacc gatcgttttt gttctgttca atggtgtttt ttctttctta tagctgtcat
    21001 ctacatatgt gcccgaccct ttttctttac gcagatagcc ctcatttacc aacacagcga
    21061 ttgcttgccg aatcgtatgt ctgctgacgt catatatttc ttgcagcttg aattctgagg
    21121 gaatggttgt atttacttcg tattcctcac gaaggatctt ttgttttatc tcttctgcaa
    21181 tatcttgata ttttgccatt tgccttgccc ctttttattt gtacgtacat atatcataac
    21241 gagtctgcaa ggtttattca agcaaaaatc aaggtttctt gtgctcataa ggcatacgga
    21301 acaggttcac caaattctgg aaatccttgc tcattccacg taaagcattg cgcgttcgcg
    21361 tgccgattag gattgtctaa cggatcccct ttttggttct tctctggtcg agcatgatag
    21421 atcaaaatat caaagcaccc gtcttctgaa accgtaaacg agttgtgacc aggaccaaat
    21481 tgcttatttt tttcagaaga aacgaatact ggctccgata atttatgcca tgattctttt
    21541 aataaaagat tcttgtgctc atcagcccac agcaggccca tggcatagtt ttcatctgtt
    21601 gcactaccag aatacgtaat gaatattttc ccattgcgag taaggactgc tggaccttca
    21661 tttactgaaa aaccccgttt ttcccattca tactcaggta tagaaaggag gatctgcttc
    21721 ccctttaacg tccaaggatt atccatttcg gatagataaa gattagaatt cccagtaatt
    21781 tcaggatctt gttgggccca cacataatat agctttcctt gagaagagaa agttgttccg
    21841 tctaaactga aagtttccct tttcgttttt atttgtccct tttctatcca atctgtattc
    21901 attggatcta tatgctgatt ttcaagtacg aacatgcggt gatgatgaga ttcatcacgt
    21961 atagttttat gattgcttgc agcaaagtaa atataccatt ttccattgat ataatggact
    22021 tcaggtgccc agatcaattc actcatcggt ccttcttcat gagcctgcca tacaatcagg
    22081 cttgcagcat gttctaattc attcagtgac tttgcccttc ttaattcaat cacttggtag
    22141 cctggaaccg atcctgtaaa ataataataa ccatctgtat gtttatatac ccaaggatct
    22201 gcccgttgcc gaacgatagg attattataa agtgtcttca tcataacaaa tttcctcaca
    22261 ttttctttat tctaattaca ttccaagagt atttttctat ccctacattt aattcgtcat
    22321 gtttttctat tgaaaaatag tttatttcag gttttatttt ttctggttct tcacgtgtat
    22381 ttttgtccaa cagatcgcta ctggttaatg tgtagtgctc tccttgatct agcaaattga
    22441 aatcatgttc aatcgttatc ttttgttgtt catttgtgcg attgatcaag aaaatagtca
    22501 gctgattgcc gttatctacg atcacactgt ccacataagg aacatttttt atcttttcac
    22561 aatcataaac agggcaaatg atttgttgtt tcaatacttg acctttagca tatttcgata
    22621 agtgttgcat cacataataa gttggattcc tccatgcttg tcctttagca tctgtcagta
    22681 ttaaaggtat tgtgtttact aacaatgatt gacaactaat ctttacgcga tctgcgtgtc
    22741 gtaaaatagc cagccctaat gaaccaaata ataatgtatc ttccatagta aagtaacacc
    22801 aatcgatggg actaccgact tcaaaatccc tatgtctttt ttccggttgg gcatgtacat
    22861 tccattcatc aaacgctaga tacattgttt tctcacttct caaatatcct tttacataat
    22921 cacaagtagc aacgatttct tcaatttgac gatcgaatcg ataagctttt gcaaggtatg
    22981 tcgctgtatc atcacgctca tttttaggag gtttagtcaa gtcttcttca tcatacttat
    23041 caatatagtt atgcaaagct aaataatcta cattctcata agcttcttct aatgcgatac
    23101 gatcccattc agggtacgat ggtaaacgtg gcgtagagct tccaactaag attgtttcaa
    23161 ttgaatcatc cactaacttc atgacttttg aggcttcatt tgctaaccta ccatattcat
    23221 aagcactctt agcccccact tgccatggac cgtctagctc attccctaaa caccataatt
    23281 taacgccaaa aggctttttt tgcccatttt ttattcttaa atcactccag aaggttcctt
    23341 ttggaaaatt acaatattct actaaatttt ttgcttcatc aattcctttt gttcctaaat
    23401 tcaccgtgaa tatcggtttc gcttgtacat gactgatcca tttaaaaaat tcgtggagtc
    23461 caaaagaatt tggttctata cctcgccatg ctaaatcaat tttttttggt cgttgttctt
    23521 tcctacctac agtatccacc caatcataac cagatgtaaa gtttcctcca ggatagcgaa
    23581 tgacacctaa ttctaattcc tttacataag agaggacatc ttgtctaaac ccgtcaccat
    23641 cagctgtttc atggcccggt tcataaatcc ctgtatagac gacacttccc atatgctcaa
    23701 caaatgagcc aaacagccga gaactgatct ctcctaccgt aaaatcctct ctgattttta
    23761 ctttcacttc ttcgtccaac agctttccct acctttcatt tcctatagtc aagataatag
    23821 atacaagttt ccatacgcca ccagcaaaga taaaaataaa aggagggaaa agaaatatga
    23881 gcagtacaaa tcctaataac aatacgcaac aaagaatatt tctcaaaaaa tcaaaaaata
    23941 ctttccatgc actttgttca aaaatttgta aaaatgtgat atctttgttt tcagaaatca
    24001 catatatcca ggataaaaga aacatggaat aaataagtaa aactacatat ccactaattg
    24061 aaagtacgat tgtctgttct tctaataatt gattaacaag aaaataaata atgatcgtta
    24121 acagactaag gaacaagttc ttaccaaaat gttgctttat tccttgaaag aaaaaataaa
    24181 tgatatttcc tgatccctca cctttccatt gcgccattac ataaaaaaac agcacatgta
    24241 gcaggcacga tagtaataac aggcaaacac acgattaccc agagaatacc taataagatg
    24301 taatcagaaa ccacaatcaa ctgtccataa agtttagtct caactatttt catcttcctc
    24361 actctttcat tccagacata gctgttcctt gaacaaaata tttctgtgca aaaatataaa
    24421 ccactagtaa tggcagcatt gaaataaccg ttccagccat tacaggtcca taaaaagaaa
    24481 tgttctgccc tgtaaataaa gctaaccctg ctggtaaagt tctcattttt gaactacttg
    24541 tcaatatcaa tggataaagc aagtcattcc aactattcat cagcgtgaaa atccctaatg
    24601 ataaaagagt aggtttgctc aatggcaaca tgattttcaa aaaaatatta aactcactta
    24661 atccatcaat gcgtgctgcc tcttccaaag aatctggtaa agtaataaaa aaggaacgca
    24721 tcatgaagat accaaatgca gttgtcatac gaggaatgat caaacctgca taagtatcaa
    24781 gcatgccaag caaattgact tcaataaata gaggaatcat gaagacttgg aatgggatca
    24841 tcattgtcag caaaacgaaa tagaacaaaa ctgattttcc tttgaaacgc atacgagcaa
    24901 atgcataccc cgcaagagaa tcaaagaata cagaagtgat cactgtccct ccagcaaaaa
    24961 tcactgtatt tttgatgtaa tctaataaag ggatcgttgt ccaaacctta atatagttat
    25021 ctagggtata gctatctgca aatattctag gcggaaatga gataatgtct ttttcgtgtt
    25081 taaaagaaga aataatcagc cacaaaaaag gaaacgcaat aaccaacaat ataactagca
    25141 tactaatcgt tcgaatgatt tttttaccac taactgcttt tcgactattc acagtaactg
    25201 taatcgagct ttctggtgcc atacaggttt ccctcctatt tcatttgttt ttctttccgt
    25261 gacatgtaca cattagtgga gatagctata gttgcaacaa tgataaacaa aaccgaagac
    25321 agtgctgatg catagcccaa ttcataaggt gctgtaaatc ctcgactgta aatatattga
    25381 acagctgttt ctgtcttaaa ttgtgggcca ccagcggttg caacatatac ttggtcaaac
    25441 acttgcatcg aaccaatcag attcgttaaa atacaaaaac ctaaggaggg aacgatctga
    25501 ggtagcgtaa tgttaaaaaa ctgctgtcga tttgttgcac catccatttg agcagattca
    25561 tacaaagagg gagagattcc ctgaatagca gtcaagagaa tcgtcatagt aacaccaaaa
    25621 ttcttccaaa cagtcatgat ggctaccgtc ggcatagcaa gggttggatc gcgaaaaaat
    25681 tgagggatcg gcaagccgac ttgtgaaaac aagtaaggaa tcgcccctac attcggatca
    25741 agcaacatag aaaaaataat cgcaattgac gtcaatgaac aaataactgg aatgtaaaat
    25801 gtcgaacgac aaaaacgatt gaatctggta tttttagata atgctactgc tgctaataaa
    25861 ccaacgataa tttgtgttgg cgtttcgagt acggtgaaat ataaagaatg aatcaacgaa
    25921 ttaactgctc gttcatcact aaatatacgt gtgaaatttt caaaacttac aaaatttctt
    25981 gatgtaaaga aaatattcaa atctgtaaaa ctaatagcaa atgttcctaa aagcggtatg
    26041 acagtaaata ataataaaac taaaaaagat ggagttaaaa acaaatacgc agcacgatca
    26101 ggacgattcc aatatttggt gaatccgttc atatagtcca cctcatcttt cattttgata
    26161 aaatatacaa tcttggttta aacgaatctg aataagatat ccgaacaatc gctgtgattt
    26221 ttgcaataac acctatctgc aaatactagg tcgatcattc agatatctgt atcttgccaa
    26281 cagtctatcc tgtataaagt tctgtactat ttttgcagta attgattgat ggcatcatct
    26341 gcatcgttca tgagtgtctc aggatcttct cctgctaata atttttcaat cagtggattg
    26401 atgatatcgt tattgatcgc agagatttgg gtaaaacctg gtaaatatgg ctctgcatag
    26461 tccatttgtt tcgataattc agatacaata ggatcatttt ttacttcttc atcttttgct
    26521 actgaatcca agtaggcagg aaaaccattt tcgatactcc attttttacc aatttcagtt
    26581 gtattccaat atttcaaaaa ctcatagaca gcttcttttt tattattatc tgtacttgcc
    26641 ggaatggcat atcctacgcc atctaaaata gctgtttttt tgtctgaatc actcccttgt
    26701 ggtaaggtag tcacattata atcaatctcg tttgctttca atccgttatt taaccatggc
    26761 ccattgattt caatggctag ttgaccagca ttcattaaat tgtccatttc ggcgccagaa
    26821 atcgattctg gaccagtctt gtctacatgg atcatttttt ggatcgcttt taacgtttta
    26881 aggttctcag gagacgcaaa ttcagactta gtataatcat cgttcactaa tttaccgcca
    26941 tttgctaaca tccagttata taacggtgca gttccatcct tattgaagac aaaacctgat
    27001 acatttttac tactatcagt taattttggt gccatttctg tcaattcttc ccaagtagta
    27061 ggtggttgtt cagtatctaa tccagcttgt ttaaacaaat cattattcca atacatatac
    27121 atgccttgta cctgcatggg gacaaaatac tgtttacctt ctacttcacc taattcaata
    27181 gccgtttctt taaaatcaga tttatctact ccatcatatt cccagaaatc gtctaatggt
    27241 tgtaccgctc cgttttctac atactgtgca aaatctggat aatttaatgc aataaagtca
    27301 ggtgcttttt tagaagaaat cgctgtagga agtttttcat tcaaattggc ccaagtcatg
    27361 atatccattt caattttgat gtttttatca tttgttttat taaattcatt aacgatttct
    27421 tgcaatattt caccatcaga tgcagtaaac ccattccaaa aagtcaaagt cacttcttta
    27481 tcagaatcag attctgctga acttccgcca ccgcaagctc ctaaaatacc tgttgcagct
    27541 accaacaaag agccaattaa ccatttttta cctttcattt tttgttcctc cttcatctga
    27601 taaacgacat ctgaccacat tccaagatag cggtttcaat aaacaattga ttttcccatc
    27661 ttcagctttt acatttgttg aatgacttgg ttttacttgt tcacttttag gaagattagt
    27721 tgcttttgta tcaaatccac tcatttcaga aaaatcaatc actccctcta atgataattc
    27781 agacaagtga acagttagat tcatttcttc ttctccacgg ttaactgcaa atatgacaag
    27841 ttctttgtca tttattacgg ctatactttc taaatacggc acttcttcaa aatcttccgt
    27901 cgcatagttt gctgaacgaa tttgtgggct taacacctct ccttgtccat acacagcagc
    27961 ttgcataaat ggataataga tcgtctgtct ccaagcttct ccgtttgttt ctgtcctgat
    28021 tggtgcaata acatttacca attgagcaag acaagcaatc tttactctat ctgcatgttt
    28081 caataaagtg attaatagac agcctaatag taatgcatct tcaaaattat agttatcttc
    28141 tagtaaagga ggagcaatct gccaaggcgc tgcttgctca tcttgttccg ctgaatgata
    28201 ccaaatattc cattcatcaa aggacaggtt gatctgcttc ttactatgtt tcttcgcctt
    28261 gactgtatcg cacatcgctg tcacttcaga aatgaaccga tccatgtcta aggaacgtgc
    28321 aagataatga gaaagatcat ttttttggtt gccataatat tgatgcagag acaaataatc
    28381 tatttgatcg tacgcttgat caagaactgt taactcccaa tcaccaaatg taggcatttg
    28441 ccgattcgaa cttccgcata aaaccacctc aattgaatca tctacgagtt tcatcgcctt
    28501 agccgtttcc tctgctaacc gtccatattc ttccgctgtt ttatgtccaa tctgccaagg
    28561 accgtccatt tcattcccta aacaccatgt cttaattcca aaagggctct cttgaccatt
    28621 cgaacgcctt aaatcactcc aataagttcc tttaggaaaa ttacaatatt cgactaaatt
    28681 acgagctgca tctactccac gagtacccag attgacagcc atattgattt cggcccccac
    28741 atgctgacac cagttataaa attcatgaat cccaacttca ttcgtttcaa ttgtccgcca
    28801 agctaaatcc aaccttctag gtctatttgc tatgggccca atactgtctt cccaacgaaa
    28861 tcctgacaca aaatttccac caggatatct aatcatggga atgttcaatt cttttaccaa
    28921 gtgaatgacg tcttggcgaa atccactttt attagctgat ggatgatctg gctgataaat
    28981 cccttcatat actgctcgtc ccatatgttc aataaatgaa ctgtacaacc gcggatcaac
    29041 tttgcctaca cgcttgacac tactaatcaa cattgtggct tccataactg acctccttta
    29101 atttatacat gaataataca cctcgttgaa agcgttgtca ataatataaa tgttaaactt
    29161 ac
//
LOCUS       DAZKOR010000031        29099 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000031 DAZKOR010000000
VERSION     DAZKOR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.031"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACG8ZM_001246"
     CDS             complement(<1..1031)
                     /locus_tag="ACG8ZM_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0492216.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACG8ZM_001247"
     CDS             complement(1113..1802)
                     /locus_tag="ACG8ZM_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0492217.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG8ZM_001248"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACG8ZM_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0492218.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG8ZM_001249"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACG8ZM_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0492219.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG8ZM_001250"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACG8ZM_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0492220.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG8ZM_001251"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACG8ZM_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0492221.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG8ZM_001252"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACG8ZM_001252"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0492222.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG8ZM_001253"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACG8ZM_001253"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0492223.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACG8ZM_001254"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACG8ZM_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACG8ZM_001255"
     CDS             complement(7636..8523)
                     /locus_tag="ACG8ZM_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0492224.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG8ZM_001256"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACG8ZM_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0492225.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG8ZM_001257"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACG8ZM_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0492226.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG8ZM_001258"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACG8ZM_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0492227.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG8ZM_001259"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACG8ZM_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0492228.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACG8ZM_001260"
     CDS             complement(10454..10957)
                     /locus_tag="ACG8ZM_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0492229.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACG8ZM_001261"
     CDS             11554..12519
                     /locus_tag="ACG8ZM_001261"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0492230.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACG8ZM_001262"
     CDS             complement(12618..13790)
                     /locus_tag="ACG8ZM_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0492231.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACG8ZM_001263"
     CDS             complement(13777..14922)
                     /locus_tag="ACG8ZM_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0492232.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACG8ZM_001264"
     CDS             complement(14936..15868)
                     /locus_tag="ACG8ZM_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492233.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACG8ZM_001265"
     CDS             15985..17064
                     /locus_tag="ACG8ZM_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0492234.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACG8ZM_001266"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACG8ZM_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACG8ZM_001267"
     CDS             complement(17789..18016)
                     /locus_tag="ACG8ZM_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0492235.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACG8ZM_001268"
     CDS             complement(18119..20707)
                     /locus_tag="ACG8ZM_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0492236.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACG8ZM_001269"
     CDS             complement(20727..21386)
                     /locus_tag="ACG8ZM_001269"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0492237.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACG8ZM_001270"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACG8ZM_001270"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0492238.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACG8ZM_001271"
     CDS             complement(22159..23025)
                     /locus_tag="ACG8ZM_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0492239.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQLVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACG8ZM_001272"
     CDS             23134..23775
                     /locus_tag="ACG8ZM_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0492240.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACG8ZM_001273"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACG8ZM_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0492241.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACG8ZM_001274"
     CDS             complement(25216..26115)
                     /locus_tag="ACG8ZM_001274"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0492242.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACG8ZM_001275"
     CDS             complement(26117..26914)
                     /locus_tag="ACG8ZM_001275"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0492243.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACG8ZM_001276"
     CDS             complement(26892..27596)
                     /locus_tag="ACG8ZM_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0492244.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACG8ZM_001277"
     CDS             27727..28302
                     /locus_tag="ACG8ZM_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFQ0492245.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACG8ZM_001278"
     CDS             28420..29097
                     /locus_tag="ACG8ZM_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0492246.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8565 a         6166 c         5231 g         9137 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctaa ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgctttaaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacaagt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaa
//
LOCUS       DAZKOR010000032        27772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000032 DAZKOR010000000
VERSION     DAZKOR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.032"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(547..909)
                     /locus_tag="ACG8ZM_001279"
     CDS             complement(547..909)
                     /locus_tag="ACG8ZM_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492247.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(983..1363)
                     /locus_tag="ACG8ZM_001280"
     CDS             complement(983..1363)
                     /locus_tag="ACG8ZM_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0492248.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1445..2620
                     /locus_tag="ACG8ZM_001281"
     CDS             1445..2620
                     /locus_tag="ACG8ZM_001281"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0492249.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2697..3281)
                     /locus_tag="ACG8ZM_001282"
     CDS             complement(2697..3281)
                     /locus_tag="ACG8ZM_001282"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0492250.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3482..4480
                     /locus_tag="ACG8ZM_001283"
     CDS             3482..4480
                     /locus_tag="ACG8ZM_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0492251.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4592..5101
                     /locus_tag="ACG8ZM_001284"
     CDS             4592..5101
                     /locus_tag="ACG8ZM_001284"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0492252.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5131..5790)
                     /locus_tag="ACG8ZM_001285"
     CDS             complement(5131..5790)
                     /locus_tag="ACG8ZM_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0492253.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5792..6556)
                     /locus_tag="ACG8ZM_001286"
     CDS             complement(5792..6556)
                     /locus_tag="ACG8ZM_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0492254.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6564..7241)
                     /locus_tag="ACG8ZM_001287"
     CDS             complement(6564..7241)
                     /locus_tag="ACG8ZM_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0492255.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7278..8663)
                     /locus_tag="ACG8ZM_001288"
     CDS             complement(7278..8663)
                     /locus_tag="ACG8ZM_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0492256.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8873..8957)
                     /gene="ffs"
                     /locus_tag="ACG8ZM_001289"
     ncRNA           complement(8873..8957)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8ZM_001289"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8980..9495)
                     /gene="tadA"
                     /locus_tag="ACG8ZM_001290"
     CDS             complement(8980..9495)
                     /gene="tadA"
                     /locus_tag="ACG8ZM_001290"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0492257.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9602..10459
                     /locus_tag="ACG8ZM_001291"
     CDS             9602..10459
                     /locus_tag="ACG8ZM_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0492258.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10540..10875)
                     /locus_tag="ACG8ZM_001292"
     CDS             complement(10540..10875)
                     /locus_tag="ACG8ZM_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492259.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10942..12312)
                     /locus_tag="ACG8ZM_001293"
     CDS             complement(10942..12312)
                     /locus_tag="ACG8ZM_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0492260.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12390..12926)
                     /locus_tag="ACG8ZM_001294"
     CDS             complement(12390..12926)
                     /locus_tag="ACG8ZM_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0492261.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13235..14716
                     /locus_tag="ACG8ZM_001295"
     CDS             13235..14716
                     /locus_tag="ACG8ZM_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0492262.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14819..15526)
                     /locus_tag="ACG8ZM_001296"
     CDS             complement(14819..15526)
                     /locus_tag="ACG8ZM_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0492263.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15605..15943)
                     /locus_tag="ACG8ZM_001297"
     CDS             complement(15605..15943)
                     /locus_tag="ACG8ZM_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0492264.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(16054..17016)
                     /locus_tag="ACG8ZM_001298"
     CDS             complement(16054..17016)
                     /locus_tag="ACG8ZM_001298"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0492265.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(17031..18362)
                     /locus_tag="ACG8ZM_001299"
     CDS             complement(17031..18362)
                     /locus_tag="ACG8ZM_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0492266.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18370..19050)
                     /locus_tag="ACG8ZM_001300"
     CDS             complement(18370..19050)
                     /locus_tag="ACG8ZM_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0492267.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19269..19748)
                     /locus_tag="ACG8ZM_001301"
     CDS             complement(19269..19748)
                     /locus_tag="ACG8ZM_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0492268.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19875..21554)
                     /locus_tag="ACG8ZM_001302"
     CDS             complement(19875..21554)
                     /locus_tag="ACG8ZM_001302"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0492269.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            22102..23604
                     /locus_tag="ACG8ZM_001303"
     CDS             22102..23604
                     /locus_tag="ACG8ZM_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0492270.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23617..24279
                     /locus_tag="ACG8ZM_001304"
     CDS             23617..24279
                     /locus_tag="ACG8ZM_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492271.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24380..24583)
                     /locus_tag="ACG8ZM_001305"
     CDS             complement(24380..24583)
                     /locus_tag="ACG8ZM_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0492272.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24694..25878)
                     /gene="iadA"
                     /locus_tag="ACG8ZM_001306"
     CDS             complement(24694..25878)
                     /gene="iadA"
                     /locus_tag="ACG8ZM_001306"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0492273.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(26035..>27772)
                     /gene="yfcC"
                     /locus_tag="ACG8ZM_001307"
     CDS             complement(26035..>27772)
                     /gene="yfcC"
                     /locus_tag="ACG8ZM_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0492274.1"
                     /translation="KKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGKF
                     STQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPEK
                     GLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGGF
                     GFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLER
                     LALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNAT
                     SWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLTY
                     KSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFFV
                     MGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASVP
                     SALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADLV
                     GVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLGT
                     IFIMIAIAIGLQ"
BASE COUNT         8248 a         5797 c         4703 g         9024 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataacta ttttttaaat tttttagaaa tgtgatataa catccacttc
      301 agaatcaacg ctgttaaatt tatcatgtat ttcttccttg gtcaactata aattaagcat
      361 tactttgtga aatgattttt cgatcaatta ttattattcg ttatacaacc gttttcaaca
      421 tattttgaaa ataaaatgca ctaaaaaact atctttagca gaacatttca ttgtattttt
      481 tggaccaaac aataaaaaaa gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc
      541 tgtattttag aagaaaggca ctccctctac ttcgtaagtt ccaagaacag agcgcatttg
      601 acctggttct tcccaaatat atccttcgcc taattcttct ctttgattct tttgttcagt
      661 ttcaaagaca aacaacggaa cattttcctc tttaatatgt gtatttgtca attccatcaa
      721 actaatcttg ctaacatcga tatgaactat ttcttttaag aaattaagta tattcgctaa
      781 accagtcttt ccttcagaaa gttccgctaa cgcaaattcc aacttgtcat caattgaatg
      841 gaccaaaaat tttttagtac catcctccaa atgcaacata attgtaccag caactctttt
      901 gcttgccaaa atttccacct cgatcaactt gttctatggc ttacataaaa attctagcac
      961 attttcagaa aaaatacact ttttatttct tattttcttt ccgttctagt tcagataatg
     1021 cttcttctat ccaaataggt aactgctccg caatagtggt aaatccgatc ttacgatttt
     1081 tattcgtgaa ataccttttt ctgcgatatg gaacagctgg atttgttttt tctaacgttc
     1141 ttaaagataa actgattttt ttggaatatt catcaatgtc gatgatctga actgttactg
     1201 gctgtccgac ttttaacaat gaatggatat ttttcgtata accggattgt acttctgaaa
     1261 catgaataag tccttgcgtc tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc
     1321 ctgtcacttt tccttcaatt atttgtccaa ttttataagt catctacttc cctctcttcg
     1381 catttatcat tatactgtaa atagctcaaa aaaagaagcg acgaaaatga ctggaggtct
     1441 taagatgatc gaatttgata aacagatttc aagaaaagat acagaaagtg taaaatggga
     1501 cgcaattgct gagacatatc aaatggatga cttacttcct ttgtgggtag cagatatgga
     1561 cttcttgtct cccggtgggg tagctaaagc gtttagtgaa tacatcaaac atggaatctt
     1621 tggttatgcc actctgtctg ataaactata tcaggctatc attcattggg aacgccaaca
     1681 ccatgctgtg gagttgacga aagaaaatat cgtttttaca agtggtgtgt taaccagttt
     1741 agctgcagca gtacaaactt ttacaaaacc aggcgattct attttgattc atgacccagt
     1801 ttatcctcca ttttcctcta ttatagaaac aaatcaacgt cacatcgttc gaagctcttt
     1861 gctagaaaaa aataatcatt tcatcatgga cttgtctgac atggagaaaa aaatcgaaga
     1921 aaacaatgta aaagcaatga ttttatgcaa tcctcacaat cctggagggc gtgtttggag
     1981 caaagaagaa ttgaaacagt taagcgaatt gtgtttaaaa catcaagtga ttctgtttag
     2041 tgacgagatc catcaagatt taactttatt cgatcataca tttacttcca tgctaacgat
     2101 tgatcctaat ttagatgatc tactagttgc ttttacttcc gcaaccaaga ctttcaatct
     2161 agctgcaatc aaaaattcaa tggtatttat caaaaatcca gagatgaaag ctgattttga
     2221 aaaacattta gtgatgaacc aacagcacga aatcaacact tttggactga ttggaaccca
     2281 agcggcttat gagacgggtg gagaatggtt aagccaactg attccttatc tagaaaaaaa
     2341 tgttcaaaca gttaatcagt tttttgaaca gtatcttccg aaagtgagaa taatgaaccc
     2401 tgaaggaact tacttaatgt ggttagattt taccgcctat atttcagacg accaagaatt
     2461 ggagaacact cttgttcgga aaggaaaagt cgtattaaat cctggcatca cgtttggtcc
     2521 ttccggtcat gggcatatgc gcttgaatat tgcttgtcca gaagaaactt tactggaagg
     2581 acttaatcga atcaagtatg ctttagagac aatcgattaa atcatttatt caaaataaaa
     2641 ggacgagacc ttactaggtt tcgtcctttt tcataattac tccgtaccat accaaactat
     2701 tctgaaattt caattgtttc aatcactaca tcatgtacag gtcggtcttg tggaccacgt
     2761 tgtacatttg cgatttcatc tactgtgtcc atcccttcta gtacatgtcc aaagactgta
     2821 tgtcggaaat ctagccaagg agttccgcca cctttatacg cttcaatgat ttctgatggg
     2881 aatccagcac cttctagttg tccaagcata ttagctggca catttgtgtt atttacaata
     2941 aagaattggc ttccgtttgt gtttggacct gaatttgcca tagataaagc tccgcgaagg
     3001 ttgaacagtt cacgactgaa ttcatcttca aaactttcgc cataaatact ttcgccaccc
     3061 attcctgttc cagttggatc gccgccttgg atcatgaaat caggaataac gcggtgaaaa
     3121 atcacgccat cataataacc ttttttggaa agttcaatga aattttgaac cgtcttcggt
     3181 gctagttcag ggaataattg aacagtaatg tcaccacggt ttgttttgat aacagctttt
     3241 ggtccttttt cactttttaa atctagttgt gggaatgcca tattgtttcc tcctaatatt
     3301 tgcatacttc ataataataa taacataata cctaacctct cgtaaaaata ctagatttgt
     3361 tttcttttat ttttcatcac tagatacatt ctataactta ccgtttcgat ttcgaattca
     3421 aacatttttg agaagcattc tcaattaaat tctgatatag tgtgaaaagg gaggaatttt
     3481 tatggaaatc tatgacatta caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt
     3541 ttacgcagga atgcgccaag ctaaaacaaa aatcattgac agtctacctc aacttggtgg
     3601 tcaattagca actttgtatc ccgaaaaata tatttacgat attcctgggt atccagcaat
     3661 aaaagcaagt gaattaatcg atcaattaga aaaacaatta actactttta accatacatt
     3721 ccatttaaaa gaagaagttc tctcgcttac tcgtgaagac gaagttatcg agattactac
     3781 aaataaaggc atccattatt ccaaagcagt cattttagct ttaggaaatg gttcattcca
     3841 gccaagaaaa ttgaatttag ataatgctga atcatttgaa aaccacggat tggattattt
     3901 cgtgaatgat ttgatgaagt atgctggaaa aaaagtcgct attgctggag gtggtgattc
     3961 tgctatcgat tggacgttga tgttagagcc tattgcaagc gaagtttact tgattcatcg
     4021 tcgcccggaa tttcgtgcgc acgaacatag tgtatcacgc ttgaaatctt catctgtcaa
     4081 cttgcttact ccctatctca tcgatggatt atcaggcaat aatggggaac tgacagatat
     4141 ccggttaaaa aaagtgaaaa gtgacgagac gattgatttg atgattgatt ctctgatcgt
     4201 caattatgga ttctcttcta atttagaaca tctttcttct tggggacttg atagtaccag
     4261 aaatgccatt actgtaaaat cagatatgtc tacttctatt ccaggcgttt atgcagctgg
     4321 tgacatctgt acctatgaag gaaaagtgaa actgattgct actgggctcg gtgaagcacc
     4381 tactgcagtg aacaacgctt tacactatat taatccaaag gaacgaactc agcctggtca
     4441 cagcacaagc ttatacgata aaaatcttcg tcctagttga ttttttatat ctctttgcat
     4501 ttcaagttaa acccgaaagc agtttcttgc atttttttca tgttccgtta acatgaaata
     4561 gagaaagaaa aaataaaggg ggagcaataa tatggtcgtc aaaactgaga ctttagaaga
     4621 aaaggcaaga gaactattga aggaacgtgg cgtaactgta gaagatatcg cacaacttgt
     4681 catgttttta caaaaagact atatttctga tttgactttg gcggattgta ctgaaagtgt
     4741 caacagcgtg ctaacaaagc gtgaagtaca taacgccatt atcactggaa tccaattaga
     4801 tatattagca gaaaagaaaa aattgatgag tccgttgcaa catattatcg aagatgacga
     4861 aggcctttac ggtatcgatg agatcctagc cttgtcaatc gtgaatgttt acggtacgat
     4921 cggctttacg aattatggtt atatcgacaa ggtgaaacct ggtattttaa aagaactaaa
     4981 cagccacgat ggcgttcatg tccatacctt cttagatgat atcgtcggag cgatcgccgc
     5041 tgctgctgcc agtcgattag cacacaaaga accagaaaaa agaagtcgcc ttactcaata
     5101 attcaacaaa aaaagaaaga aaacgtctat ttagacgagt tctttctttt ttgtttgttt
     5161 caagctattt tttcctaaga aaagaaaaac agcaaaaaat aattctaata atacaaaaaa
     5221 taagataaag ctatgcatga aaaattcttc tggaagtaca ttgatgattg gatcagtaac
     5281 gggatcgaat aaccagtcat cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg
     5341 atcaaatcct atcatcatga aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa
     5401 cggacgaatc aaccgccaaa gccgatcatt cttttgcaaa tatttgataa acaaaatact
     5461 cgggatcaaa gtgatgaaaa acacagcata atccactaaa aacaaaattt tcacttcata
     5521 aaagtgatgg gctccgctag cagaaactgg gaaatctggc aagctgagga tggttttgaa
     5581 gggattattg agataattca ttaggtgatc aaaatttttc agtaacgtct cttgatctaa
     5641 agaagtataa tccaaaattt gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt
     5701 gatcgtcaca ccaactgcca gactgatcag tgtcagaaat aggctgaaca atccagcata
     5761 ttccatccac tgccatctcg aatttttcat attagaagct ccattcatcc agtgattgtt
     5821 tcacataggt agctgggata ggcaacccag gtacatcttc ttcttttgta aaacctgata
     5881 aaacgagcaa agtatctata ttattgcgta tcccagcttg gatatctgtt tcatagttat
     5941 ctccaaccat gattacttct tctttttgca gccctaatac ttcaacggct ttatccataa
     6001 tgatggcttc tggttttccg atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag
     6061 agatgactga ccctgctcca ggaagaaggc cgcgttcagt aggtatattc ttatctgggt
     6121 tcgttccaat gaaagtagca cctttttgaa tagcaagtgt tgctttaact actttttcat
     6181 aggtcagata attatctaag cctaccacta catagtcagg agcctcttct tcccatgtaa
     6241 atccggcagc taaaatcaag tcaatcaatc ctgcttctcc gataacatag acttttttcc
     6301 cttttccatc tgctttcata aaatcgattg ttgccaaagt agctgtgtag actgtctctg
     6361 gagctacatg aatgtcaaac tcatctgcta gacgattagc tactgtttct ggtgacttag
     6421 tcgtgttatt ggtaacaaac aagaaaggta attttctttt ttgcaattct tccacaaatc
     6481 gtttaccggc cggtatgggt tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat
     6541 atcctttata gtccattcat ccactactct tccctttttc tgatcgtaaa tgctgttttt
     6601 gttttttcta cttgtttagg ttgttcttgt ttttttactt tttcagaggt ttttgctgac
     6661 tctgtttttc gatttttaat aaccggtttt ttgcgattag atgcgactgg tccttttttc
     6721 tcatcgatat acgcacgatt atggtttttt ttcttttttc ggcgctgagt tgtcttttct
     6781 tttttaccgc cgattctctc gatgacaaaa tacgcacaac caaaattgca atattcgtaa
     6841 agatagtctt ccaaggaatc gattcgctga tctggatgcg cccgtcgatt atccgcttgg
     6901 aaaaatcctt ttaggcgtag ttgttcataa ccccaatctc ctacgatata atcatatcgg
     6961 gctagtacat cactgtaacg ttctccaagc ttttctgcat taaatccttc acgataatcg
     7021 gacacaagcc gataatgtct ttcacctatc aaaaaattac tgtcatctaa cagtgtcact
     7081 agctcgcctt tgactttttc tgctttaggc tcctcaacga tttcttccaa agcagctgtc
     7141 agttcttcgg tgactgttgt ttcctgttca gctgatgatt ttttcttagc aatttctgtt
     7201 ttatgctcaa cgttctgctg ttcattttgt tttgcagaca tgcgttcacc acctatcttt
     7261 tttattatac cttgttttta tttgattggg tagtgagctt tcaagtaatc tcctaccaca
     7321 cttcggataa attcagggaa caaaaattca ttttttccag caatctcaat cgttggaaaa
     7381 aatggaacaa acatcaaatg atctacggta gtaaaagtat aatttttatt cagatcgatt
     7441 ggcttgttct tccaaaagac ttgatggtta atagaatcat atgtgattcc attatagacc
     7501 atctgcccaa aaatcttacc gcgaaatccc attcccttca taggatagtt acgtaaaaag
     7561 ttacgatttt tttctatttc taaaactaag cggatcagat catttccacc caatgtgaca
     7621 tttaacaagt gcatcggatg cggcaacgca gtgtgaagct gatcctgatt gacccgccct
     7681 tttggaattt cagttaaaaa cagaccactg tttaatattg ctgcttccgt ctttcctcgt
     7741 tctttcaccg cctcaaaggc tacttggata aacgaatgct cattgaagat atctagactc
     7801 agagaaaatg gcaaatctgc tacttctttt tcttttaata attcatgccc acgttctaga
     7861 taactattta tttcttgttc atcttctgta aaagctgtca ttgtttcggt agggatggct
     7921 ttggctgaat gcttaaggat ttttttctct tcatcgatgg ttaaatggac ttcaccgaca
     7981 tactgcccgt actttccagc ggctgctatt tggacatcat tgattacgtg accttctctg
     8041 aataaatgat gggtgtgcga tcctaaaata acatcaattg ctggtatttc ttttgcaatt
     8101 tggaaatcat cctgaatccc gagatgactc atcaaaacta aaacatccac ttgtgaacgt
     8161 aaatcttcga ctaattctgg caaaatatca taaggattcc gtatgtccca gccattcgga
     8221 ttataagtaa gcggaaatgg agcagtaaat gcaattagtc cgattttcgt ctggtgtttc
     8281 gttgtaatga ttttatatgg tttcgtccat gccggtggct ggagcgtatt tttatcgaat
     8341 aagttatcca ggatgatatc aaactcagca tgatcgtata aatgattcaa ctcttctttg
     8401 gaatttccta cgccttcatt gtttcctatc gtcgctgcat cataaccaac ttcattcatc
     8461 agcaagatat tttcctgtcc attcgttgct tctgacaatg gatgccaacg atccacaaaa
     8521 tcacctagat ctactgcaat cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt
     8581 tgatccaaaa accttctgat ccttggccaa ttttctaaat gagaatgcag atcattcgta
     8641 tgtaaaatca ctattttttc catgtaccct ctcgcctctc tttatcttta tcataccata
     8701 ggcagctttt tcttccattg aaacaagctc tatttcagag tttttcctgc cttgaagcaa
     8761 attgtataag tacaatattc gataaatcag tctggctaaa aaatcaggaa cattcatttt
     8821 tacagtacta taaaaataaa aaaaacagac ccgaaagtct gttgtcgttt cattataatc
     8881 agacacatgg cggaacctgc ttagtgctgc ttccttccag acctgacacg cttcacaagc
     8941 ccaccattgt cctagccttt cggcaaagaa tagtatacct tatttttcat tcgttgacca
     9001 gtcttttttt aatttctttt ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat
     9061 cccacattcg tcttctaaaa tgccaccttc tacatatgct tggtggttaa accgctcgtc
     9121 ctccaaaaga ttcatcaagg taccggctgt tccgcctttt ggatcatacg cgccgaaata
     9181 aacttctgct acacgagaca aaatcatcgc gccgctgcac ataggacaag gctctaaggt
     9241 aacaaaaagt gcagcatctt ccaagcgcca gcttcccact ttttcacagg cttccttgat
     9301 tgcaagcatt tctgcatgtg tagtagcatc ttgcgtggtt tctctaaggt tatatcctct
     9361 tccaatcact tttccatcca atacaaccac tgctccaatc ggaacttcat gaagcatttc
     9421 tgctttcttt gcttctgcca atgcaaggcg catccatttt tctttttcta ctgcatcaag
     9481 atttgtattt accaattacc cttccctgcc tttgtttttt cttgttttac tttagcatat
     9541 gataaaaagg ctgtaactaa cttttttttc tatggtaaaa taaatacaag aggtgaacaa
     9601 gatgaaaatg gataaattac ttgctcttta cccacaagca cagaaaaaaa actctccagc
     9661 atcagaagcg gatatccttt cttttgcaat tgatgatgct tttatctgga tcaaacaaga
     9721 ttcattatct caacaagaaa ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca
     9781 aaaaaaacac ccatggtatc actatctttt taaagatgct tcttgtgttc aagaaaaaag
     9841 ctttcgtttg atccagcttc acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca
     9901 agatactatt ttggaattat ttcgctattc tgctgatttc tttttttata cagacacaga
     9961 tgcgctctta gtcgaacaaa taaaccgtga ttatcttgat gctactgaaa tcaacggtat
    10021 ttttctatct ttagatgctg attttgatac gacaacgtct gtattcgttg gttctttcca
    10081 ttcacctggc accgacctcg tccctttgtt tgctgaagaa agaaaaattt ttttaacaga
    10141 gcaaaacaat ctttatactc acagccgtac tttttctatg caagatgtgg ctttacatta
    10201 ctatacaaaa gatactgtaa aagatagtaa gttgatcaaa agttacatag aactgatcaa
    10261 acaaaatgaa atggaagtga tcattcaaga attatggaat gaattaggca atgtcagctc
    10321 tgctgcaaaa acactgtttc ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca
    10381 agaacagtct ggtttcaatc taaaaaaagc caacgattta ctgttttgct atcttttatt
    10441 gcttcatgaa cataattaaa gatagattaa aggggttgtg acaataattt tgtcacaacc
    10501 ccttatttcg aatgaatagt gttcaaaagc tagtttctct tacatcattt tttcaacaaa
    10561 atgcattagc cggtctttta catcttcggt tgaatctttg attgaatcac cataaacaga
    10621 gattttcttt tttcctgatt tgatttttgc taacattccc atcaaagaat ccaagtcact
    10681 atctgaaaga ccatctacga tttctagtaa tttttcattt ccatcaaatt tgtcatagac
    10741 gaattttttt actttcgtcc gagtagtcat atgatggatc ttttccatta cttttcccga
    10801 tacaacgact gctgtcgcta cactggcagc ggctgctacg cttaatccga ttgttacttt
    10861 tgttgatgtt ttcataagta tcagctcctt tttatctatc tccatcatag catagacaaa
    10921 aaaataaacg aactaatcca ctcagtgaaa tctcaatcct ttagggaaaa agttctttac
    10981 tgttcctttt acttgtttcc caaatccgac aggtatctta tctaatacta gcaaaaccca
    11041 tccttgattc ccatcacgtt gaaatgtttc tcctgctgtg taagattgcc attccttttg
    11101 agtgatcggc aagcaaggta tattctctat cttttttgta gcaagcgcta atgcatagct
    11161 aggttcaaat cgattctttt tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa
    11221 accatcgaga gaaggggcta actcaggaac ttcccaaaga tggtcgttga acactaaaag
    11281 tcttccagta gcttcataat gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc
    11341 ttttgtcatt tgcactttac ttttttttcg tgtcttcttt tctttatgca tctgattttg
    11401 tccatgaaaa gtcagctttg caacaaaatg tccttcaccc tgatctttat gaggccatat
    11461 acgtattgtt ttttccaaac cagctacgct tccccattca gaacgtcctg aactaacgga
    11521 ttgagtcaaa ggaatctcct caatcgttac tggataattt tctacaagcc agctaatgat
    11581 ctcttcattt tcttctggtg caaacgtaca agtcgaataa atcagttgtc ctttattttt
    11641 tagcattttt attgctgaag acaaaatctc ttgctgacgt ttttgacagt atagcggaga
    11701 ttcttctgtc cactctttta ttgcatttgg atcttttcgg aacattcctt ctcctgaaca
    11761 aggagcatca accacgatcc gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg
    11821 tgcatgattt gtcactattg cgttcgaaac tccccatcgt tctatatttt ctgataaaat
    11881 cttcgcacgt ttcggaaaga tctcattcgt cactaataac cctttcccct tcatttgagc
    11941 agctaattgg gttgatttac caccgggagc cgcacacaaa tccaaaactt tttcaccggg
    12001 ttttgctgca gcagctgttc caacgatcat tgcacttggt tcttggctat attcataccc
    12061 agcttgatgg aggaaagact ttccattgac agtgccaaga aatccctcat tgctgtaggg
    12121 agcgggttgt aattcttcgc tatggtaagt ttggaccatg tccaatcctg ccggttttag
    12181 tggattccac cgaaatcctt ttttcacgct tccttgttca agcgcagaaa aaaaatcgga
    12241 tgcttcttcg cctaaaagaa gacgatattt tttgataaat tgctgtggta acgttgtcgc
    12301 ttcctccttc attccaatgt ttcgttagac attttacaat cttttttacg aaaaataaag
    12361 gtggctgatc tactttttca tgttgctgcc taaggtgact gttcctttaa gatattttcc
    12421 aagtattcat ccgtcacctc gtttttatcc caaacagcgt tcatcatttc aagaaaatct
    12481 tgagaagaat catctgagcg caaacttccc aatgcattaa gccataccaa ttcaagcgac
    12541 ttttcttctt tgatcttcgt gtgacctttg acatatgaga aaaatggccg cttaaatagc
    12601 gaatccatgt ctaaatcaga ttttgcaatg atttcacgaa tactttcatg gatagaaaaa
    12661 aaatctttca ataagtagac aatgcttaaa acatcatcgg aactgcattc agctaagtag
    12721 ttttcaaaat aaacagtgat ttcttctaat atcttctctt ttaacccgct gattgatttg
    12781 aaatgtgtat aaatcgggta tgtagaacaa ccgcagaact ctgctaactc tcttgcagta
    12841 attgacttca ttccatattc ttccacgtat accatacttt tgaacagcaa atattcttta
    12901 ctataatttg ttactctccc catcattctt acctccgtct cttttcatct acccataaat
    12961 aaataacata aataattcta aaaatattat tgataattat caaaacatat ttagttatat
    13021 ccattaaaaa tcaatttttc cgccttaaaa acaagaatat cttgacaaaa taccctagga
    13081 aataaacaat agaataaaca aggtaacaaa aaaattaaat aacgttatat aaaaaagaag
    13141 gcgacactct gtgagaataa cacagactga aatgactttc cactcacagt ttttgtctaa
    13201 ctatatacaa aaacagaata aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga
    13261 gttacttgtt ctacaaatgg tcattcaaaa aaataattgt tctttacaag aattatccaa
    13321 tgacttacat attccaaaac ggacaatcaa agaactgatc cgaaaaataa atgtatcact
    13381 caagcaattt ttattgattg atgaatttat ttattctaat cataaaggag agatccaaac
    13441 caaagaaacc gatcaaacta aaaagttagc tgttttttct aagttaaaat tatgctacct
    13501 aaaagaatcc aatcgtttta actatttgat gttactaatc aattatccac aaactgctgt
    13561 accaaaaaaa tatttacttg aacaacttta tatatcgccc tcctatctcg aaaaactgac
    13621 aaaacagctc aatcattcct taataaaatt ccatttacgg atcctatctg taaaaaattg
    13681 ctgtactctt gaaggaaatg agttatctat ccgtctctat ctttactttt ttctttcaga
    13741 tacatttgga ggtaacgatt ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa
    13801 cagtacagat gagcaaatag aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt
    13861 aactttattc atattacgaa acaaacaaca ggcatatttt ccaaaaactt cttctgaaat
    13921 cactgaattg atggacttat tgcagcatac acatgattat tcagaatact ttacttcttt
    13981 ttcagtgagt cgcccttgtg aagagacaga aacagagaga gcctacttta attatttttt
    14041 ctattgtttt tttccaaatc aaatctcttt tcgacaaaaa caggagattg gttttttatt
    14101 ttccacaagc aaaaacctta tttgtcgaca agtcggtcaa ttgatgactt acttaactca
    14161 acagtttcct ctcaaccatt tagaaaataa aataccaatt tatttttatt ttcttgtgtt
    14221 ggcagttgtt tttttcgaac tgttgggtga atccagcagt agtttttcta cactttgctt
    14281 tccagaagcc acgtccaatc attctagttc ttttatacag gaaaagaaat taagagaaaa
    14341 aatcgataca attttttcaa aaaatgggga aagaaaccaa atcagtcaat accttagtca
    14401 attgatccaa cacttactac aaacagaaac gaaagagcag ttactcatcc atttgcagat
    14461 aaccaaacag tttacaggaa attatcatct tcaaagccat ttgcagcagt tatttcgcaa
    14521 agatgctgtt aaaattaccg catattcaga acatgctgat ttgatcatta cagatcagca
    14581 tgagaaaatt tcagatgaaa cggagacttt ctatctggat acaacgcaca atacagtgtt
    14641 atttggcaag ttaataaaag cggttcatga actgtatatg aaaaaaggtt atgagaacaa
    14701 aacaaagagg atgtgacaaa attcttgtca catcctctta ttccaaataa atggtattca
    14761 atcagctaac tctcggtatt agctcagatc aaagcgctga tttcacaact tctttatttt
    14821 aatctcttcg gttgccgccg aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa
    14881 gcgctgctgc aacataagtc agtgctgctg caaataatac gtgtcttgcc atcggtactt
    14941 cttcttccgt cagtagtccg ccttcactca aaatttggat tgctcgccga gacgcgttaa
    15001 attcgactgg taatgtcacc aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga
    15061 tattgattaa cgtagagttc cagctaagca atacacctac cataatcaag gggaacgaaa
    15121 tcatcgaacc aaagttagct gctggaacaa gtgctgcacg aatcttcaat gggacataac
    15181 ccgaagcatc ttgtaccgca tgtccgcatt catgagctgc tacacctatc gcagcaactg
    15241 aagtagactg tgcagttgct tgagacaaac tcaacatctt attacctgaa ttgtagttat
    15301 ctgtgagatc tccggcaatt tgttgaacac cgacattatt tatctgttga ctttgtaata
    15361 tatattgtgc tgcttgtgta ccagtcacat tgtttttgct tctcacttga tcgtattttc
    15421 ggaaagtact attgacgtac gctgacgcaa ttccggaaat aactaatcca agaatgacca
    15481 aaagaaatgt cggatctaaa aaaccataca ttccataacc atacattttc gtttcactcc
    15541 ttttcgttta ctttatttta tcattagtag taggaaaaga atgtgaaaag accttgaata
    15601 ttctttaatt ctttttcaca aattccgatt tcagtttcat cggtccaaat ccttctacct
    15661 tgcaatcgat attatgatcg ccttctacta aacggatgtt cttgactttt gttccttgtt
    15721 taatggcacc tgacgcacct tttactttca agtctttaat cactgtcacg ctatcgccct
    15781 ctaacagacg gtttccattt gcatctaata ccactaatcc gtcttctatg acagtttctt
    15841 gttcttcttt gttccattcg ttcccgcatt ctgggcaaat caacaagcct cgatcttcat
    15901 atgtgtactc tgagccacat tctgggcaat ttggtaatgt catcttcttt ctcctttaaa
    15961 atctttagtg agcactattg taacggaaaa agaagatctt gaaaagatac atttgtgatt
    16021 tttactacaa aaggcacgtt ttcgaacgaa gtatcattct gtttttctgc tcttatacga
    16081 ttggcatata tcttggatct ctttatagat ttcctctact tctgctaaat cttttgcatt
    16141 cgcaccactt gctaatggat ggcctcctcc atgatggcgt ttagccaatt cattgatgat
    16201 cggcccctta gaacgtaaac gtacgcggta ataccctgaa ggctgctgaa caaaaatccc
    16261 ccaagccagc acttcgtcaa tcactccagg aagtgaaacg atcgaagctg tctctgaatc
    16321 atcgattcca tatttatcaa gaatttcttg cggaaggatt attcttcctg caccattttc
    16381 atccacctgg atattttgat aaagataacc ggaaagttta gcgactttca tcggcatttg
    16441 ttcaatctct ctgttcaaag cacttgcatc aaatggataa tttctcaata tcgaagcaat
    16501 ctctaatgta cgggacgtcg tggagggata caaaaagcgc cctgtatctc cgacgattcc
    16561 tgcataaagt aaccgtgcag ccttaacgtt catctttaaa gattcaggaa acatcaaagc
    16621 aaattctgca atcatctcac tacagctact tgcttttgta tttacccaca caagatctcc
    16681 ataagggtca tcattgggat gatggtcgat tttgatcagt ttttctgcca actgatatct
    16741 ttgatcactg attctgggtg aattagctgt atcagtcacg atgactagcg ctccttggta
    16801 gaagacatct tcaacttctt gcatttctgc taaaaagcga agcccttcaa tcgtttcacc
    16861 tacttgacgt atttcttttt ctgggaaact ttcccgtaat agttcggcta atcctacttg
    16921 tgatcctaat gcatcaggat cagggcgcaa atgcctgtgg ataatgattt tatcgtattt
    16981 ttttattgta tctactattt cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt
    17041 tccattactt gacaaacaac gattgctttg gaaacgatgg tgttttcaat aaacacttcg
    17101 atatccagtt tagcggaacg tctgccgatt tccaatacac gtggacgaat gtctaattcg
    17161 ctttccaatt ggatcagccg caaataatgt aggttcactt gttcaatcaa gatatttcgt
    17221 ttttggttca tcaccattgt tttttgtgcg acatcagaaa tgatttcgct aagtacgcca
    17281 aaagaaatgg ttccaacgcc attgaccatt tgtggtgcta ctacgaattt gaacttaggg
    17341 ttttccaaac gatttccttc acggtcagta tcaatcgtat ggatatttcc agatatctga
    17401 tccgaaatcg tatctgcaat ctgcggttgt cgctggacaa gctgcatcgc tttcattaca
    17461 tcttgccggg taatcaagcc ttccaatgtc aagtcatcgg aaacgaccgg catgacttct
    17521 agcccatccc agatcatttg atggctaaca gaggcaatac tcattgtttt ctttacactg
    17581 attggttctt tcgtcatcac ccgatcgatg atctggtttg gcgatttccc taaaacatct
    17641 tttgccgtaa caatccccat caaacgatga tggcggttga cgactggaaa acgcgaatgc
    17701 tgcgtttctt ctgacaaacg ctgataatca gaaatcgtat ctgtctgctg caaataatga
    17761 gtcttttcag ctggcatata gatatcactg acaagcatga tatctttttt gatcaattgg
    17821 tcgcttaacg ctcggttgat catggttgcg actgtaaatg tatcataaga ggtgcgtaag
    17881 atcggcattt caagatcatc agctagcttt gcaatctcag cagtcgtatt gaatcctcct
    17941 gtgatcaata ctgcggcgcc atcttccaat gctaattttt ggacttccgc ccggttgccg
    18001 acgatcatta gtgatcctgg cgtaatatag cgttccatgg catgtttagt catcgcgccg
    18061 atgacgaatt tattcaagac cttgtctaat cctgccgccc cgcctaaaac gtccccttca
    18121 atgatcctga cgacctcgcc aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg
    18181 atacggatgg tccctactcg ttgaattgtt gaaactagtc cgacattttc tgcatctttg
    18241 attgcacggt acgctgttcc ttcactgaca ttcaattctt ttgcgatgct tcttacagaa
    18301 attcgattgc caacaggtaa actttcaata tattctaaaa tcaaatcatg ttttgtcgcc
    18361 attttctgcc tacacttcca gcgtttccga gacgctcatt atttttcctt cactatcttc
    18421 cagtaactct acaaaagatt cgggatcttg ttcaatgaca gggaaagtat tgtaatggat
    18481 tggaactact ttcttggcac gaattgtttg cgcagccttt gctgcatctt ctggccccat
    18541 cgtatagtta tcgccaatcg gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa
    18601 tcctagatca ctgaaataag cagtatcccc tgcatgataa atcgtttttc cttctgcttg
    18661 aataataatt ccgctaggct cgcccatata ctgaacgata ccgtctactt catagcttga
    18721 actatgctgt gcatgtacga attttaccac gccgaatgga aacgcatgtt ttccaccgat
    18781 attcataccg aatgaatcta ctcctctgct gttcgcataa tcagcaatct caacaatact
    18841 gataattgtg gcttgatttt cttttgcaat aggaatcata tctcctatgt ggtcactatg
    18901 accatgagta atcagcaacc aatctgtctt tacttcttct gcagttaagt ctgtcaatgg
    18961 atttcctgta ataaacgggt caatcatcac gcgttggcca ttttccattt caattgttaa
    19021 acaggaatgt ccatgatacg taattttcat attttacacc tcgcgttttc tctgtatctt
    19081 ttttattaaa aacgatttca tcacttgcac atcaatgaaa aaatatagct gctaaatata
    19141 ttttcattgg attgatagta cagtatgtac aaagcaattg taacagataa tacaggatgc
    19201 accaagtacc aagcagatat aatctcaagt ttgaggtctc tgctctgctt agcttctctt
    19261 ctcgtccctt aactaattaa atcagcgtaa ataggatgga cgatcttttc taaatcggct
    19321 acttttatcc gaaggcttct cccgatttca ccgcttgaca caattacttc ttctttttct
    19381 tttaaagaac tgtctgctaa aaaacaataa tcaggatgtg cgagttttat tccaataggg
    19441 gtgtttgctc cgtggggata tcctgtatgc attaatacaa aatccattcc aggaaaacca
    19501 actttacgat ctttcgtcac ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga
    19561 atcaatgcga tgatcacccc agttttgttt cctttcacta cgagagtttt acatatcaaa
    19621 gctggatcaa tccctctttt ttgtagttct tctgtgaaag attcttctac cacatgacta
    19681 aaatcaagtg gttgatagct gatcttttgt tcttctaaat aacgttcgac ttttgtttga
    19741 ctcatcattg attcactccc tttattctat cgtttattat aaagcgaggg atgctgtatt
    19801 caaaaattat ttgtttttcg caaaaaaaga gcaagacgag cactctatag agaggttcat
    19861 cttactcttt ttgtttattt tttatttcga cgttgagtag cttccataat gactggcaag
    19921 atcactgggc ggcgttttgt ttgttcaaat aagtaacggc ctaattgatc tcggatgtct
    19981 tgctttaatt tgctccactc aaaatcattg ctttctaaat gtttctcaac gattgtttca
    20041 atcatattgc tgctttcttt catcagatca cggctagctt ttacgtaaac gaatccccgt
    20101 gaagtaattt gaggttttga aatgattttc ttttctcgcc gactgatagt gacaaccgct
    20161 acgaaaatcc cgtcttctga caagattttt cgatcacgta aaacaatgtt tccgatatca
    20221 cctatgccta atccatcaat cattacattt tctgcacttg tagttcctgc aacactcata
    20281 cgcttgttct tatattccag cacgtctccg cgtccagtaa tatagatatt tttgtatggc
    20341 atccctactg catgagccaa atctgcatga gcagctaatt cacgatactc tccttgaact
    20401 ggcacaaaat atgttggttt catcaaattg atcattaatt gcaagtcatt aggattggca
    20461 tggccagaaa cgcgcatatt ctcagagatc aacttcactg ttccaccagc tcgataaatg
    20521 atatcttccg ttttcgccac gatggtttcc atcgcgatgc ttggcgtagt agtgatataa
    20581 accaaatctc cttcttcaat cttgacatta cggtgtgtac cgtttgccat tttttgcaga
    20641 gacttgatgg gttcacccat tcggccagtt tctaaaatga tcagttcttc tggatcatag
    20701 tttttcatgt ctttcaacgt aacaagcaga tcttcacttg gtaattgaag tttttctaaa
    20761 cgcatcgctg tgcgaataat tcgttcgaaa tcttgaccag tcaagacaac ttttcgttgt
    20821 gctttttctg ctgcatccaa tacttgttgc actcgttgca aattacttgc tacacaagcg
    20881 acgatgatac gcccttccca atatttgatc gtatcaaata cttcatctgc tatttgggct
    20941 tcagaagcta caggctgagg attttctgca tttgccgatt cacttagtaa ggctaagact
    21001 ccttctttgc cgatttccgc caatcgtgca taatctgttt gatacattgg gatagccgtt
    21061 tggtcaaatt taaaatctcc cgtataaacg atactacctt cttttgtttt cagattgatc
    21121 cccactgaat ctggtatcgt atgagtggta cggaaaaagc tcactactgt ttcaccaaag
    21181 tctatttctg tatgctcatc taccacatgg aaatccttaa atcctttggc tgcttcatta
    21241 cgactgacat tcaattttgc taactcgatc gtcaactctg tcccgaatac tggtacagaa
    21301 atttttgaca acaagtaagg aagtgccccg atcgcatctg catgtccatg tgttaaaaag
    21361 acaccagcta cacgatcaat gttttcttct aaatacgtaa aatcagggat tacgacgtcg
    21421 atccccaaca actcattttc aggatatttt agcccacaat ctaaaataaa aatatcctcg
    21481 cccacttctg cgacatacat gttttttcca ttttcacgaa cgccgcctaa tgggataatt
    21541 ttaatggtac tcacaaactt cacctcttct tatgacatcc aagtttaaag atgtcgattt
    21601 tcttctttat ctaaatatat gagccattcc aatgaagcat atgcaaaatc actatttctc
    21661 aacttatgga gaatcagcag gaaaattacc agcacatccg cgttctgaca gttggatggg
    21721 cattttattt tggatatact tcattaacaa aatggatctt ttgcgaaacg ctatagccag
    21781 aatacttata taagtattca gctgttcccc attattatac aacaaataaa ctaaatcgct
    21841 agtatcagca attgttagaa caactatgca cgtaaaaatc cctctgtagc atctaggatt
    21901 tcttttgcta ttcctctttc atttcttctt cgtcggtttg aaacatactt ttttcatttt
    21961 accacatttt ttgcaataac tatagtgact ttttatatat acagaacctc atgttgatgg
    22021 tgtgcaaaca gcataagatt taagctagct ttaagatagg ttgtctatta tcaaatgcat
    22081 ctactaggag ggatgcaaaa tatgaatttt atgaaacgtg catggaaaag tacaaaagta
    22141 aaatggggac gttcattgtt attgtttgcc gtgttcacag ccattcttat ttttgtgcta
    22201 gctggcctca cgattcgaag cgctgcagaa gaagcagcga atcaagcaca aaaagaagtt
    22261 ggagcaacag tgactttaag tgccaatcga gaagcttcat ttaaaaaaca ggaacaaact
    22321 tcttcaagcg gttctaccac tgaagaacgt ccagatccag gcagtttttc tcttacacca
    22381 gtttccttaa cggacgcgga aaaaatagct gaattagata atgtaaagag ttattccttt
    22441 gaaagttccg cctcagctat ggcaaaagat ggaataactg ccatcactag ttctgattca
    22501 agtgaagaca gttcagaaac tactgatgca ggcggtgaac ctgctggagg aatgggcggg
    22561 aatccgcaaa tgactcaagc agactttcaa atcagtggtg tgtcagcaac tgcacaaaat
    22621 agtaattttt ctgatggaac agcaaaaatc acggatggtg aaggaataga tgacagcgac
    22681 aaaggcacaa acaatgtagt cattgattct actctagcag aaacaaatga cctggcagtc
    22741 ggagatactt tcacaatcac gagtactgaa gatgaagata ccacttatga aatgacgatc
    22801 aaaggaattt atgaatccag cgaaacaagc ggcagtatgg gtatgaaatt caacttcatg
    22861 aatccgtcga atacacttta cacttcttac acttttgcaa atgaactaaa tgggacatct
    22921 gacgataaca cgatagattc tgctatttac actctatctg atccagataa aatggatgag
    22981 tttctcaaag aagctgagaa gctgatcgac acagatacat ttagcttaca atcaaatgat
    23041 tccatgtatc agtcaatgtt ggaaccttta aacaacgtgg caagtttttc taaaaatgtt
    23101 gttttactag tggctgtagc cggtattatc atacttactc tgatcattat gattaccatt
    23161 cgggagcgtc ggcatgagtt aggcgtcttg ctatctttag gagaatccag aagtaaaatc
    23221 attctccaat tgtttaccga agtggctatt tgtatgattc ttgcattagg agttgcttcg
    23281 cttagcggaa acgtcgtcgc gaatgccgtg ggacagcagc tacttgacca acagacagaa
    23341 accacgacac aaaaccagca agctcctggc ggtggacaaa tgccaggtaa cagacaagaa
    23401 caaggcggtc aacgcgatgg tcaacaagga accacgaata atccatttgc tgtttctgat
    23461 caagtcagcg aattggaaat caatgtcaaa cctgcacaat tgggactatt agccggagta
    23521 gcatttggaa tcagtctgtt ttctgtcttt ttagcttcta tagggatatt aagattaaac
    23581 ccaagaaaaa tcctattaaa ctaaaggagg aatttgatgt tacggacaga acaattggga
    23641 ttttggtatc aagatccgca agatgcgctt tataaagatg tagaactgaa tttcgaagct
    23701 ggaaaaatgt acgctatttt aggagcaagt ggttctggaa aaacgacttt tctttcgctt
    23761 atttctggac tagatgcacc aaaagaagga aaggtatact atcaagatcg ttcactgaac
    23821 aaaatcgggt taagaagtta tcgtaaaaat gatgtctcga ttattttcca agcttacaac
    23881 ttgcttcctt atttatcggc tctagacaac gtcatagcag caatggaaat cactaagacc
    23941 aagcaagaga attatcgaca agttgcttta gatcgactgg ctcgtgtagg aattgatgaa
    24001 atattagcaa aaaaacaggt gaccaaactt tcgggaggtc agcagcagcg agtcgctatc
    24061 gttcgtgcta tctgctgtga acatcaacta atcgtagcgg atgagcctac tggaaatcta
    24121 gatgaaacaa cttcacaaga tatcgtcaaa cttttccaag aaatcgccca tgaacaaaat
    24181 cgctgtatta tcatcgtgac tcatgagatt gaagtagcaa aaagctgtga tgaagtcttt
    24241 gaactaaaaa ataaaaaatt tcaaaaaatc gaagtttaaa aaacaggata atgatcaaat
    24301 aaaaaatccg aaccatatct gtctcgcaga tatggttcgg attttttatt tgcttgattc
    24361 aattagtaat aagtggtttt tatttttttt cgtcttttcg agaaaacttg tctttgactt
    24421 cgtcgcctaa atttcgagct ttttctttga tgtcagcacc tagtgattgt gctttccctt
    24481 ctgtttcttt gctcttgtcg tctgttgctt tgccgtatgc ttcttttcct tcaccgacta
    24541 ctttatcttt catatcttta gaacgtcctt ttaaatctgc cattgtgctt cctcctctta
    24601 gtttgtttat tgatctacct tcagggtaac atcaacaaca gatacagaca aacagaacgc
    24661 tcgttttcta aaaaagatag ttagagctgt tgattaaaaa cttttttcga aagtccccca
    24721 tactttgact tctttttctt ggaccattat ttctccttta gcgataactg tatcaatatc
    24781 aagcgtttct ttatccaaga tacacagatc tgcatcagca ccaatttcga tttttccttt
    24841 tttatccaat ttcaatatac gtgctggatt actggttata gctcgtaagg cgatttccaa
    24901 agggatactt tctttttgca cagcttcttt gatccctact agcaatgcct tggcacttcc
    24961 tactcctaac cctaggaaat gattgttttc atcaaaataa ggcaagcttc cttgtccatc
    25021 tgaggtcatc gtgaaattat ctaaagaaac atcttcctcg atcaatcgtt tcagtgcttt
    25081 gctgaagcga acttcaccat ctgtcttctc ccaaaaatca ggatcttcgc tagcagtgaa
    25141 gtcaatcgtg ccaccacgtt tggcaaatgc cacgcaagct tcaaataatt ctggtgtgcg
    25201 atttgcatga gtaggcaaaa attgtgtgat cggaatttct gtttcttcta cgattcgtgt
    25261 caatagctct agctttcttt ttcctccgcc taagtggaca ttgatgatcc cagcttttcc
    25321 ggataacatg cctcctacac gagcatctgc tactgcacga gcgaactctt caaaacttgg
    25381 ctgagaagaa cggtgatcgg atactgcgat ttctccgatt ccgataattt tatcaattgt
    25441 caaaaagtct ttgatgatag aatctgttac tgtttttacc ggcaatcggt acgaaccttc
    25501 gtatacataa gtggagattc cttctgcttc cagacctttc gctttgctga tcaacgcagt
    25561 catatctcta ccttctccgt ctgttcccaa acaaccgaca actgtcgtta caccagctgt
    25621 agtcaattgg ctcaatgtga cttctggcgt tcgattttga taaccgcctt ctcctcctcc
    25681 acctaaaata tggaaatgac agtcaatgaa acccggggtt agtacttttc cctcacctga
    25741 aagttcttct acttccactc cttcgtagcc gccttctagc tgttcttcta cagccaaaat
    25801 cttcccaccg gcaatcagta catccatttt tcctaagtaa tctggtgcaa acacttcaat
    25861 ttgtcgaatg attttcatac atttttcctc tttctttaag tataactgat gataggatct
    25921 taaaaaataa tccaaacgat aggatcttta ctcctttgat tttaccgtaa aatgacggtt
    25981 cttggagtcc ttatctactg tttggattac ttttttattt acaggtctcc agtactactg
    26041 cagacctatt gcaatggcaa tcatgatgaa aatagttcct aaaacaaaca ggaatccttg
    26101 catcttgatt tggaatcttg cccattttgc ccattcgatc cgtgcaacac ctagcacacc
    26161 aatcaagctg gctgatacgg gtgtaaaagc gtccataaaa cctgaaccaa gctgataagc
    26221 aagaactgct acttgtctgg atacacctac taaatctgcc agtggagcca tgattggcat
    26281 agtcaaagct gcttgtcctg aatttgatgt aacaaccaag ttgaacaaac tttggaagat
    26341 gtacatcgcc caagcaccaa tgacagctgg aacaccggtc aacgccgtcc cgatcccatg
    26401 caaaatcgta ttcaaagctg aaggaacaga tgcatcagat cctcccaaga caagaagaat
    26461 ccctttggcc atacctacta cgattgctgt accagctaaa tcagctgcac cagattggaa
    26521 agaagaagcg atgtcattga ctttcattcc atctaatttg aaaagaacag caatgattcc
    26581 agcaactaat cccatcacaa agaattgaga agcgatttct ggaatgtaat agcctttttg
    26641 agtcacgccc caaacgatcc agacaaggac aactaacatc tctaataaga tcaatttatg
    26701 cccaagcatg aattttttat tttcatcaga tgtctttttg atatgagaac ggaaataatc
    26761 atctgatttg taagtcaacg aactttctgg ttttttacgg attttttctg catatatcat
    26821 catataacca gcggctaatg cagtaacaac tacccacata atcaatcgga atgtcgcacc
    26881 agacaacacg gggattccag caatcccttg agccaccgct acgctaaacg ggctcatcca
    26941 agaggtcgca tttccgactt gagatgcgac ataagtcacg gttactgcta cgatcgagtc
    27001 atatcctaac gcaatgacaa atggaaccat caccattgag aatggaatta cctcttctgc
    27061 catcccgaat gtagcgccac cgaatgaaaa agcaaagaat aatagcggta aagctaaacg
    27121 ctccagtccc ttagttttac tgataaacgc atagatacct gcgtcgatcg ctccggtacg
    27181 cataataata ccaaaagcac ctccaacaac aaggatcaat gcagcgatcc ctacagcaga
    27241 tccatactta tctccagata ctagtccttc aaaaacaaaa tttaagaaac caaagccgcc
    27301 aaaatcatct gttccccata ttttggctgt tttatgcaat tttttagaag tatcatagat
    27361 attttcgccg tactgatcgt aaagcacatc atctgtcaag gagatttcat ctagtttttc
    27421 ctgcgtaaga tttttttctc cgtcagcaat gactttttcc aatccttttt ccggaacatc
    27481 cagtttttca cgttctgctg gatgatcttg cagttcttct aattgatcaa acacatacga
    27541 cttatccagc tcgtaagcat aacggaagga atcttgacgt aaaacagtac gagtacttgt
    27601 ttcgccattt gcatctttgt attcaatatc ttgcgtgctg aactttcctg ctggaacgac
    27661 aaaagtcagt atccatgcaa agatgactac accaaaaata atgacatacg tatggggtgt
    27721 cgataattta gtgaagtcgc gtttcggttt tcctgaagaa tttgttttct tc
//
LOCUS       DAZKOR010000033        27659 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000033 DAZKOR010000000
VERSION     DAZKOR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.033"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..966)
                     /locus_tag="ACG8ZM_001308"
     CDS             complement(22..966)
                     /locus_tag="ACG8ZM_001308"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0492275.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1262..2206)
                     /locus_tag="ACG8ZM_001309"
     CDS             complement(1262..2206)
                     /locus_tag="ACG8ZM_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0492276.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2427..3641
                     /locus_tag="ACG8ZM_001310"
     CDS             2427..3641
                     /locus_tag="ACG8ZM_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0492277.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3663..5678
                     /locus_tag="ACG8ZM_001311"
     CDS             3663..5678
                     /locus_tag="ACG8ZM_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0492278.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5761..7134)
                     /locus_tag="ACG8ZM_001312"
     CDS             complement(5761..7134)
                     /locus_tag="ACG8ZM_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0492279.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7160..7444)
                     /locus_tag="ACG8ZM_001313"
     CDS             complement(7160..7444)
                     /locus_tag="ACG8ZM_001313"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0492280.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7446..9461)
                     /locus_tag="ACG8ZM_001314"
     CDS             complement(7446..9461)
                     /locus_tag="ACG8ZM_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0492281.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9640..9945)
                     /locus_tag="ACG8ZM_001315"
     CDS             complement(9640..9945)
                     /locus_tag="ACG8ZM_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0492282.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10034..11065)
                     /locus_tag="ACG8ZM_001316"
     CDS             complement(10034..11065)
                     /locus_tag="ACG8ZM_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0492283.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11272..12147
                     /locus_tag="ACG8ZM_001317"
     CDS             11272..12147
                     /locus_tag="ACG8ZM_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0492284.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12299..13141)
                     /locus_tag="ACG8ZM_001318"
     CDS             complement(12299..13141)
                     /locus_tag="ACG8ZM_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0492285.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13233..15110)
                     /locus_tag="ACG8ZM_001319"
     CDS             complement(13233..15110)
                     /locus_tag="ACG8ZM_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0492286.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15107..16528)
                     /locus_tag="ACG8ZM_001320"
     CDS             complement(15107..16528)
                     /locus_tag="ACG8ZM_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0492287.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16531..19920)
                     /locus_tag="ACG8ZM_001321"
     CDS             complement(16531..19920)
                     /locus_tag="ACG8ZM_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0492288.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20177..21568
                     /locus_tag="ACG8ZM_001322"
     CDS             20177..21568
                     /locus_tag="ACG8ZM_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0492289.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21641..22288)
                     /locus_tag="ACG8ZM_001323"
     CDS             complement(21641..22288)
                     /locus_tag="ACG8ZM_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0492290.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22296..22982)
                     /locus_tag="ACG8ZM_001324"
     CDS             complement(22296..22982)
                     /locus_tag="ACG8ZM_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492291.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(22988..24061)
                     /locus_tag="ACG8ZM_001325"
     CDS             complement(22988..24061)
                     /locus_tag="ACG8ZM_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0492292.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24283..24957
                     /locus_tag="ACG8ZM_001326"
     CDS             24283..24957
                     /locus_tag="ACG8ZM_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0492293.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            24962..26302
                     /locus_tag="ACG8ZM_001327"
     CDS             24962..26302
                     /locus_tag="ACG8ZM_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0492294.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26451..27437)
                     /locus_tag="ACG8ZM_001328"
     CDS             complement(26451..27437)
                     /locus_tag="ACG8ZM_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0492295.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8064 a         5224 c         5019 g         9352 t
ORIGIN      
        1 ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt
       61 tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac
      121 accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc
      181 agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat
      241 acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata
      301 agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc
      361 tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac
      421 ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa
      481 tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac
      541 acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt
      601 tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg
      661 catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat
      721 accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac
      781 agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc
      841 tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc
      901 ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt
      961 tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct
     1021 tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa
     1081 acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat
     1141 aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa
     1201 taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt
     1261 cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg aataatttga
     1321 ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg
     1381 tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg
     1441 aagcatcaaa atacattttc aatttattac taagatagac cggattgtag ccaaaatgag
     1501 ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa
     1561 gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag
     1621 tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg
     1681 tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc
     1741 cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag
     1801 aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt
     1861 cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag
     1921 cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa
     1981 tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg
     2041 gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc
     2101 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
     2161 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat
     2221 tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca
     2281 cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg
     2341 gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga
     2401 taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc
     2461 tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta
     2521 tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct
     2581 cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa
     2641 aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct
     2701 cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga
     2761 tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta
     2821 tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag
     2881 gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta
     2941 ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg
     3001 gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt
     3061 ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga
     3121 cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg
     3181 caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc
     3241 ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc
     3301 tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt
     3361 acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa
     3421 tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct
     3481 cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt
     3541 tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa
     3601 gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta
     3661 aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc
     3721 gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact
     3781 ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac
     3841 cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac
     3901 atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag
     3961 gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg
     4021 gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg
     4081 atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg
     4141 aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag
     4201 aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg
     4261 tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga
     4321 aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc
     4381 aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt
     4441 cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt
     4501 tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat
     4561 cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga acgatattag
     4621 cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct
     4681 tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca
     4741 tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag
     4801 ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag
     4861 aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt
     4921 gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc
     4981 aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg
     5041 gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt
     5101 tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct
     5161 tattgaatga cactatcaca cagattacta ctccatattt caaattctat gaacaagatg
     5221 ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag
     5281 ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa
     5341 atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag
     5401 caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt
     5461 atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac
     5521 ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg
     5581 atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc
     5641 cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat
     5701 atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc
     5761 ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa
     5821 tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc
     5881 tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc
     5941 gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct
     6001 attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc
     6061 agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa
     6121 gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa
     6181 tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg
     6241 tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac
     6301 cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat
     6361 tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc
     6421 aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa
     6481 gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt
     6541 tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt
     6601 gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt
     6661 tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac
     6721 taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa
     6781 tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa
     6841 agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac
     6901 tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact
     6961 gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg
     7021 gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac
     7081 gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag
     7141 ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt
     7201 cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg
     7261 tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa
     7321 catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt
     7381 tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt
     7441 tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt
     7501 aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga
     7561 agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga
     7621 ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg
     7681 ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca
     7741 tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt attttctgat
     7801 aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat
     7861 tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct
     7921 aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct
     7981 tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat
     8041 cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc
     8101 atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta
     8161 gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc
     8221 tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag
     8281 ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc
     8341 gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg
     8401 ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa
     8461 gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata
     8521 cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca
     8581 aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa
     8641 aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa
     8701 aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta
     8761 atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt
     8821 tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt
     8881 gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa
     8941 taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc
     9001 atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc
     9061 ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt
     9121 atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga
     9181 aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct
     9241 tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt
     9301 gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat
     9361 tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt
     9421 tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac
     9481 ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg
     9541 atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg
     9601 accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt
     9661 ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt
     9721 ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa
     9781 agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc
     9841 ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat
     9901 tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag
     9961 tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat
    10021 tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca
    10081 aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta
    10141 atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt
    10201 cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat
    10261 cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat
    10321 cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt
    10381 tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat
    10441 cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat
    10501 ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat
    10561 agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat
    10621 aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact ttttggactg
    10681 tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt
    10741 ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta
    10801 gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg
    10861 tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat
    10921 attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat
    10981 tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat
    11041 tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata
    11101 tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac
    11161 acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga
    11221 tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt
    11281 gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac
    11341 atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg
    11401 tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa
    11461 gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg
    11521 attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg
    11581 gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta
    11641 gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat
    11701 tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc
    11761 atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc
    11821 gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg
    11881 aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa
    11941 caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact
    12001 ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct
    12061 caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc
    12121 cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag
    12181 tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac
    12241 ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct
    12301 aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca
    12361 gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta
    12421 cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat
    12481 gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga
    12541 ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga
    12601 gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg
    12661 tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc
    12721 cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat
    12781 tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg
    12841 tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat
    12901 ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt
    12961 ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga
    13021 tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta
    13081 ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca
    13141 tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc
    13201 cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca
    13261 aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc
    13321 ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc
    13381 aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat
    13441 ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct
    13501 gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat
    13561 acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct
    13621 tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct
    13681 actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt
    13741 gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg
    13801 ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa
    13861 ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg
    13921 tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca
    13981 aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt
    14041 ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca
    14101 ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca
    14161 tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt
    14221 gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt
    14281 ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca
    14341 ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt
    14401 gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct
    14461 tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt
    14521 tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta
    14581 tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca
    14641 gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc
    14701 gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca
    14761 gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt
    14821 gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa
    14881 actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg
    14941 ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga
    15001 gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa
    15061 ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc
    15121 ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc
    15181 ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt
    15241 tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg
    15301 taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc
    15361 gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt
    15421 gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat
    15481 ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg
    15541 tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac
    15601 caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc
    15661 ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga
    15721 tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct
    15781 tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac
    15841 tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg
    15901 aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa
    15961 aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt
    16021 tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga
    16081 cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt
    16141 tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt
    16201 ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta
    16261 ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg
    16321 tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat
    16381 gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt
    16441 ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac
    16501 acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa
    16561 aagataccaa atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc
    16621 aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg
    16681 gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga
    16741 cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt
    16801 aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt
    16861 ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc
    16921 tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag
    16981 tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta
    17041 aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc
    17101 ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc
    17161 aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt
    17221 tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc
    17281 atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt
    17341 atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt
    17401 gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag
    17461 cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc
    17521 atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag
    17581 atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt
    17641 agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc
    17701 tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat
    17761 ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt
    17821 atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc
    17881 atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt
    17941 gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga
    18001 tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc
    18061 gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg
    18121 gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt
    18181 tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga
    18241 aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct
    18301 tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg
    18361 atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg
    18421 agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag
    18481 accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa
    18541 cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat
    18601 tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct
    18661 gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag
    18721 caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag
    18781 tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt
    18841 tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct
    18901 tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc
    18961 gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc
    19021 tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg
    19081 agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat
    19141 cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt
    19201 aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg
    19261 atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg
    19321 atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa
    19381 ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc
    19441 ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc
    19501 aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc
    19561 tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt
    19621 aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact
    19681 taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc
    19741 atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat
    19801 tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt
    19861 tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat
    19921 ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa
    19981 acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa
    20041 gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta
    20101 atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga
    20161 attaaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat
    20221 gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat
    20281 cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg
    20341 gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca
    20401 aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga
    20461 gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg
    20521 caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac
    20581 tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta
    20641 cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa
    20701 gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat
    20761 tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc
    20821 tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa
    20881 tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac
    20941 gattttttca gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa
    21001 tatgtcctac tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt
    21061 tcaattatta acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt
    21121 gacgatccgt gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc
    21181 tggatttcgt gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta
    21241 tttatctagc tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc
    21301 tgataaaaaa atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga
    21361 atatatcagt gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga
    21421 agatcagctg tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat
    21481 tttgaatgcc catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga
    21541 agaactcatc cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc
    21601 tgataaaaat ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa
    21661 tccaacgaga tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa
    21721 tgagatccgt caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt
    21781 cttccgacga ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca
    21841 ttggagaatc agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta
    21901 acaagtataa cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat
    21961 atttttcgat ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt
    22021 gatggactat gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa
    22081 aatatttata aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga
    22141 cttggctgat gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct
    22201 ttgcgttcga taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga
    22261 ttatacgtgg atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct
    22321 gtcaagtacc catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca
    22381 tgagtaacca taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg
    22441 acttcatatg catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt
    22501 ggctcattat ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct
    22561 ttaggataac tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt
    22621 gttgaatctt ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt
    22681 aaaaaaggaa tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct
    22741 gctcgttttt tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa
    22801 gtcggtgttt gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga
    22861 ccaatgatgc tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag
    22921 acctttgtat gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc
    22981 atttttatta acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa
    23041 acaatccgcc tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc
    23101 aaaaaacagc aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa
    23161 gagcaagcag aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga
    23221 tgaagaaatt tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg
    23281 tcatcacgta catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca
    23341 tcgcatttaa ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa
    23401 tagttaataa ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta
    23461 ttacgatccc attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca
    23521 aagcagtcca cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag
    23581 gaaagatacc gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa
    23641 ttttatagcc tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt
    23701 catgcccttt cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa
    23761 cattttcttt gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc
    23821 ctccttcaat gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg
    23881 catcctccga caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt
    23941 ctgaaagtcc attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa
    24001 taccgacgat caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca
    24061 tttgtttttc ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta
    24121 actctaaagt gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga
    24181 catctttcga taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct
    24241 ttccatgata aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta
    24301 gtagaagacg ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag
    24361 ataacacaag tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat
    24421 cttgcaatct tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata
    24481 cggaaagaaa aaatacagac tccagtattg attttgactg caaaagatag tttggaagat
    24541 aagattgaag gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa
    24601 gaactgatca tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat
    24661 gttctgaagt ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa
    24721 caagttttgc ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag
    24781 ggaatcattt tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg
    24841 acgattacag ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga
    24901 ttggataaag aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc
    24961 aatgaatgga gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc
    25021 atttgctttt atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc
    25081 atacagagaa acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt
    25141 agagatacaa agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa
    25201 cagaacaaac atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt
    25261 gatcttgtgg tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa
    25321 ccaaatcaaa gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt
    25381 aaatgaagac agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac
    25441 gaatcaagat ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc
    25501 attagcgact tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat
    25561 tggtatcagt tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag
    25621 acaaaaagaa tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca
    25681 aaatagtttg gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag
    25741 catcgctcaa gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac
    25801 aatcgctcgg aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga
    25861 atatatcgaa caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt
    25921 tattctccat aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt
    25981 tttggttatt ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt
    26041 ggattcatct gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat
    26101 cagtgaagaa gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc
    26161 gaaagagact ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca
    26221 tcatggacag atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt
    26281 gccgttgaag cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt
    26341 cacagccctt ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc
    26401 cgaaatttct ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt
    26461 ttattgcggt cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta
    26521 acatcttctg tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca
    26581 cgaataactg tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg
    26641 tcatcttcca atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg
    26701 atgacgattt cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt
    26761 ttgtggatgt cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct
    26821 tttagttgaa ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt
    26881 gtacccattg tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc
    26941 aatggatccg ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca
    27001 gtgccagttt ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc
    27061 attgtatctt ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca
    27121 tcagaaatat ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa
    27181 tcataggtat caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca
    27241 ccatccatcc gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt
    27301 ctagatgctg cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta
    27361 cgcaaccgtt caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt
    27421 aaatttttct cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt
    27481 acttataaat ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg
    27541 attaaaacaa tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata
    27601 aaatgtcatt tgtttctata tgaattttta tgaaaaattt catataattt ccactcgtt
//
LOCUS       DAZKOR010000034        27577 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000034 DAZKOR010000000
VERSION     DAZKOR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.034"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACG8ZM_001329"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="ACG8ZM_001329"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0492296.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="ACG8ZM_001330"
     CDS             1286..2461
                     /locus_tag="ACG8ZM_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0492297.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="ACG8ZM_001331"
     CDS             2465..3106
                     /locus_tag="ACG8ZM_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0492298.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="ACG8ZM_001332"
     CDS             3103..4020
                     /locus_tag="ACG8ZM_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0492299.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="ACG8ZM_001333"
     CDS             4017..4688
                     /locus_tag="ACG8ZM_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0492300.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="ACG8ZM_001334"
     CDS             complement(5229..5717)
                     /locus_tag="ACG8ZM_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0492301.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="ACG8ZM_001335"
     CDS             complement(5918..6184)
                     /locus_tag="ACG8ZM_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0492302.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="ACG8ZM_001336"
     CDS             complement(6184..6447)
                     /locus_tag="ACG8ZM_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0492303.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6968..7108
                     /locus_tag="ACG8ZM_001337"
                     /pseudo
     CDS             6968..7108
                     /locus_tag="ACG8ZM_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(7250..9073)
                     /locus_tag="ACG8ZM_001338"
     CDS             complement(7250..9073)
                     /locus_tag="ACG8ZM_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0492304.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="ACG8ZM_001339"
     CDS             complement(9671..12376)
                     /locus_tag="ACG8ZM_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0492305.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="ACG8ZM_001340"
     CDS             complement(12682..13629)
                     /locus_tag="ACG8ZM_001340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0492306.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="ACG8ZM_001341"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="ACG8ZM_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0492307.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="ACG8ZM_001342"
     CDS             complement(14264..14626)
                     /locus_tag="ACG8ZM_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0492308.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACG8ZM_001343"
     CDS             complement(14637..15758)
                     /gene="alr"
                     /locus_tag="ACG8ZM_001343"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0492309.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACG8ZM_001344"
     CDS             complement(15774..16124)
                     /gene="acpS"
                     /locus_tag="ACG8ZM_001344"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0492310.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="ACG8ZM_001345"
     CDS             complement(16254..17765)
                     /locus_tag="ACG8ZM_001345"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0492311.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACG8ZM_001346"
     CDS             complement(18087..19439)
                     /gene="murF"
                     /locus_tag="ACG8ZM_001346"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0492312.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="ACG8ZM_001347"
     CDS             complement(19592..20668)
                     /locus_tag="ACG8ZM_001347"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0492313.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="ACG8ZM_001348"
     CDS             20864..22609
                     /locus_tag="ACG8ZM_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0492314.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22889..23326)
                     /locus_tag="ACG8ZM_001349"
     CDS             complement(22889..23326)
                     /locus_tag="ACG8ZM_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0492315.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23310..24683)
                     /locus_tag="ACG8ZM_001350"
     CDS             complement(23310..24683)
                     /locus_tag="ACG8ZM_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492316.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25005..25493)
                     /locus_tag="ACG8ZM_001351"
     CDS             complement(25005..25493)
                     /locus_tag="ACG8ZM_001351"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0492317.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25515..26117)
                     /locus_tag="ACG8ZM_001352"
     CDS             complement(25515..26117)
                     /locus_tag="ACG8ZM_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0492318.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            26216..27555
                     /locus_tag="ACG8ZM_001353"
     CDS             join(26216..26657,26657..27555)
                     /locus_tag="ACG8ZM_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0492319.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         8090 a         5747 c         5086 g         8654 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc
    13741 gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 ttgatagcat caatcaattt attcttttct ttttccaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc
    19801 cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt
    21241 agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg
    21961 tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca
    22801 tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt
    22861 acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa
    22921 aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc
    22981 ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt
    23041 cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat
    23101 aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac
    23161 tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat
    23221 aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct aattttatga
    23281 catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc
    23341 ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt
    23401 gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat
    23461 aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa
    23521 aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc
    23581 attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg
    23641 tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat
    23701 aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa
    23761 atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa
    23821 atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg
    23881 aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat
    23941 tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa
    24001 atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat
    24061 aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg
    24121 tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa
    24181 aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct
    24241 tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc catattgata
    24301 aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca
    24361 ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt
    24421 ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct
    24481 agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc
    24541 gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa
    24601 ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca
    24661 aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa
    24781 aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag
    24841 cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa
    24901 gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc
    24961 ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt
    25021 ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta
    25081 atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc
    25141 aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa
    25201 gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt
    25261 ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag
    25321 taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc
    25381 atgtaatcca cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt
    25441 atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca
    25501 cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg
    25561 ctatacacac ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa
    25621 gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc
    25681 aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat
    25741 ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca
    25801 agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat
    25861 ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg
    25921 atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg
    25981 ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc
    26041 aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact
    26101 gatccttttt tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
    26161 tatgatgtgc accccaaaag ttagactaga aatctaatta aaaggggtgc tttttttgcg
    26221 gaaatataca tttgttttta agaaaaaagt agtttcagac tatttaaata acgaaggcgg
    26281 ctacaaatat cttgcacata aatatcaaat aaatcgtaca ctggttagac attgggtaag
    26341 gatttataac tatcatggtt gggaaggctt ggttggaggt ggcaaaagct acactacaaa
    26401 atttaaactt gatgttatag aatatatgga aacaaatggt ctttctatcc aagaaactgc
    26461 taaaaaattt aatatcggtt caaatagaac tctaagtaaa tggatagagc aatatgaaga
    26521 aggcggtgct tcttcacttg agagccaaaa aaggggcaga aaaattagta tgaattccaa
    26581 gctaaacatt cctaaaaaac ttaaagatga gtctcttgaa gaagaagtta ttcgtttaag
    26641 agcagagaac gcatatttaa aaaagttaga aaccttgatt caagaacaag atttgagcaa
    26701 gaagaaatca aggttaaaac aatccttcag ctcaaaaaag aatttaaatt gaatctatta
    26761 ctttcaattg cacaattagc gaagtcaact tattattact gggtaaaaaa attagataag
    26821 ccagataaat atagcaagat aaagcaagaa attacagcga ttgtaaaaga atcaagaaac
    26881 tcttatggtt atcgtagagt cactttagcg ttaaagatga aaggatatac aatcaatcat
    26941 aaaacagtta gaaaattaat gtcccaaatg ggacttacct gtcaaatccg aataaagcga
    27001 tataaatctt ataaaggaac agtaggaaaa attgccaaga atgtgttaaa acgaaatttt
    27061 tcagtagata cacccaataa aaaatgggtg acagacgtca ctgaattcaa gataaaagga
    27121 agaaaaatct atctatctcc tatacttgat ttatttaacg gagaaataat tagttatagt
    27181 atatcaacta gtccaacata taaactgatt gaagaaatgc ttcaacaagc aattaaaaaa
    27241 aagggaactg agggttcact gattctacat tcagatcaag gatggcagta tcagatgcca
    27301 caatatcaaa aaaaattaaa agagaataac attattcaaa gcatgtctag aaaaggaaat
    27361 tgcttagata attctgtaat agaaaatttt tttggtgtgc ttaaatcaga gtttttttat
    27421 cgagaaaaat tccgatcaat tgagatattt caaagtaaat taaatgagta tattaggtgg
    27481 tataataaca aaaggataaa actaaagtta aatggactat ctccagtaga atatcggaaa
    27541 cagtccatta aatagttcaa cttttggggt tcagttc
//
LOCUS       DAZKOR010000035        25447 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000035 DAZKOR010000000
VERSION     DAZKOR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.035"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..160
                     /locus_tag="ACG8ZM_001354"
                     /pseudo
     CDS             32..160
                     /locus_tag="ACG8ZM_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            274..513
                     /locus_tag="ACG8ZM_001355"
     CDS             274..513
                     /locus_tag="ACG8ZM_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492320.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            625..819
                     /locus_tag="ACG8ZM_001356"
     CDS             625..819
                     /locus_tag="ACG8ZM_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492321.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            905..1576
                     /locus_tag="ACG8ZM_001357"
     CDS             905..1576
                     /locus_tag="ACG8ZM_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0492322.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1629..3605
                     /locus_tag="ACG8ZM_001358"
     CDS             1629..3605
                     /locus_tag="ACG8ZM_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0492323.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3620..4372
                     /locus_tag="ACG8ZM_001359"
     CDS             3620..4372
                     /locus_tag="ACG8ZM_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0492324.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4388..5143
                     /locus_tag="ACG8ZM_001360"
     CDS             4388..5143
                     /locus_tag="ACG8ZM_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0492325.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5156..5416
                     /locus_tag="ACG8ZM_001361"
     CDS             5156..5416
                     /locus_tag="ACG8ZM_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492326.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5413..7503
                     /locus_tag="ACG8ZM_001362"
     CDS             5413..7503
                     /locus_tag="ACG8ZM_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0492327.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7530..7994
                     /locus_tag="ACG8ZM_001363"
     CDS             7530..7994
                     /locus_tag="ACG8ZM_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492328.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            7991..8233
                     /locus_tag="ACG8ZM_001364"
     CDS             7991..8233
                     /locus_tag="ACG8ZM_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0492329.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8251..8553
                     /locus_tag="ACG8ZM_001365"
     CDS             8251..8553
                     /locus_tag="ACG8ZM_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492330.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8623..10686
                     /locus_tag="ACG8ZM_001366"
     CDS             8623..10686
                     /locus_tag="ACG8ZM_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492331.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10686..13298
                     /locus_tag="ACG8ZM_001367"
     CDS             10686..13298
                     /locus_tag="ACG8ZM_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0492332.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13295..15826
                     /locus_tag="ACG8ZM_001368"
     CDS             13295..15826
                     /locus_tag="ACG8ZM_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0492333.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15876..16208
                     /locus_tag="ACG8ZM_001369"
     CDS             15876..16208
                     /locus_tag="ACG8ZM_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492334.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16209..16802
                     /locus_tag="ACG8ZM_001370"
     CDS             16209..16802
                     /locus_tag="ACG8ZM_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492335.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            16965..18989
                     /locus_tag="ACG8ZM_001371"
     CDS             16965..18989
                     /locus_tag="ACG8ZM_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFQ0492336.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            18989..20188
                     /locus_tag="ACG8ZM_001372"
     CDS             18989..20188
                     /locus_tag="ACG8ZM_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0492337.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            20204..20848
                     /locus_tag="ACG8ZM_001373"
     CDS             20204..20848
                     /locus_tag="ACG8ZM_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492338.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            20859..21665
                     /locus_tag="ACG8ZM_001374"
     CDS             20859..21665
                     /locus_tag="ACG8ZM_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492339.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            21687..21917
                     /locus_tag="ACG8ZM_001375"
     CDS             21687..21917
                     /locus_tag="ACG8ZM_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492340.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            22431..22739
                     /locus_tag="ACG8ZM_001376"
     CDS             22431..22739
                     /locus_tag="ACG8ZM_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492341.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            22741..23163
                     /locus_tag="ACG8ZM_001377"
     CDS             22741..23163
                     /locus_tag="ACG8ZM_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492342.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            23183..24772
                     /gene="mobP2"
                     /locus_tag="ACG8ZM_001378"
     CDS             23183..24772
                     /gene="mobP2"
                     /locus_tag="ACG8ZM_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0492343.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            24784..25119
                     /locus_tag="ACG8ZM_001379"
     CDS             24784..25119
                     /locus_tag="ACG8ZM_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492344.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            25260..>25447
                     /locus_tag="ACG8ZM_001380"
     CDS             25260..>25447
                     /locus_tag="ACG8ZM_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492345.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         9499 a         3935 c         5233 g         6780 t
ORIGIN      
        1 cgtaagaaac cgttaggttt agctaagtgg tgagtagttc acaatgacgc ttacgaatga
       61 taatggagac gaattatctg ttgaagatac ttggtcatat ttgagtgatt atattgtcag
      121 cgttgaaatc attgaatttg tagcggataa agaagaataa aaactggagg aataaatttt
      181 acagataaag gcttagaaga agtcacgacg aataataaaa aggtagtatt accttaggca
      241 ttgagtaagg aattcattta ggaggaaaat tagatggcgc gaccagtttt agaggcgatg
      301 gatatggata ctttgacaaa aggaaggtat gtacagctgt tagctcgtgg ttattcacct
      361 aaagatgtat tcaaatccct gtctaaagga acagatatgg aaaaagaaag attacgaaaa
      421 gaattcgatt attggagaac ccataatgga atcaaggatt tgaaaccagc acggccagta
      481 attaaacgaa agaaaaagat aacccaagac tgaagtgtag agcgtatgtg atcataggct
      541 tttttatttt tgcaaaaaac aaggaaggta ggaaaactca tgaattatga agaaatcgaa
      601 aacagaaaaa aagtgtcgaa ataaatggaa gaaaagttgc taaaaacgat gaaacaaaaa
      661 caccttaaaa gattatctgt catgcaatat atcaatgata tgcagataac aggaaaagag
      721 aaagcttgtt tgttaggttc aatgaaaaac tttgaacagt tgagacgaac ttatgtgaaa
      781 acaagttcta attgtcaact attgttagaa gtctcatgac attcattaat agaattcatt
      841 tttaaaactt tttctatgga aatgagaaaa agattttttg ttttgtctga aagggggtag
      901 ctggatgaga aaatggttat ttggtttttt aggagtcgct ttaatcgtct tttgcagtgt
      961 gtttggctat gttagctacc aaaaacatga aggagaggtg tttaagcaaa atatagaaaa
     1021 aaagatgccc gtagatcaaa tcaatgcaca tgcaaagagt tataaggagg atgcaacgaa
     1081 cgtcaataat gatatgtctt tggggcaaat gttgtccata caacaagaag caatcgaaat
     1141 gggcgtcaat aaacaagtat ttgcacaaat tcagattcct gcacttggtt tagcattgcc
     1201 aatttttaaa ggggcaaatc aatacacttt gagtcttgga gcggctactt atttttatga
     1261 agatgctgaa atgggcaaag gaaactatgt cctagcaggt cataacatgg aaatgccagg
     1321 tgttttattt tctgatattc aaaagttgtc attaggggag gtgatggact tagttagtaa
     1381 cgatggcgtc tatcgttaca aggtaacgcg taaatttatc gtacctgagt actttaaatt
     1441 aatcgatggc gttcctgaag agaactcttt cttgtctttg ccaaagaaag gagagaaacc
     1501 cttattaacg ttgtttactt gtgtttatac gtcacaagga aaagaaagat acgtggtaca
     1561 gggagaacta caataacaag gttttaatca acgtgagtcg ttgaaatatg aaatgaggag
     1621 agtaaaaaat ggcaataaat cggtatgtaa aaaaagctgg aatgcttatt ggaatgttag
     1681 gaatcattgc aggctgcttt cagggatatc gagcatatgc agaagatgtc acgcaaaaaa
     1741 cgccaccaga gaaggtcaat atcacggttc ataaattaat gtacgatcaa ggaacgcaat
     1801 tgaatgttga tattgacggg atcaaaaacg atggttatac gcacgatcaa tatcctacag
     1861 gtgtaacgaa atacaacaag gcagattatg gtgatgttga gtttacgtta ggaaatatta
     1921 cggatcaagt attaccgaga gaagattctg atctaaccaa cgcgaaagtg gacgagattg
     1981 tacaagatgt agaagacaag ggatctaaca gtgagtacgt caagaatgca accaacaaaa
     2041 ttacttcagc tgtagatgaa aatggtgaga tcacttttac tgatcaacca gcctatgtca
     2101 attccaaagg gaatgtatat gtggtttatg aaagtaaatc cgctgctgga ttagtcacac
     2161 aaaaagcaaa accaatggtt gtgattgcgc caatgacaga taaaacagga tcaggatttt
     2221 taaaaaatat tcatttgtat cctaagaaca ttgtttctaa gttgagtttt gaattaacga
     2281 aatttggtga cgatggcgca gaccaaagca aacaaaccct tttaaaaggg gcaaaatttg
     2341 agttatacaa gggcgaacct ggaaaaggaa caaaattggg tgatcttgta tcggatgatc
     2401 aaggaaagct gactgcgact gatttgacgt taggaaaata ctattttgtg gaagttcctt
     2461 cggaagttgt agtgggatca gatcaagaac caacagcaga tcaatattta ttaggcgcag
     2521 acgctcgcaa cgatgcgcac aataaattaa cctttgaaat tacgaatgat ggcgtgacaa
     2581 gtgacttaaa agcttcatac gtaaactata aagcaccagt gatcgataaa acagtaacaa
     2641 atggaacagg acaagaacat tctttccaaa ttggtgacgc agtaaattat caaggaacga
     2701 ttcatattcc aacagacatt gcaggtggcg cagacggtat cacagtaaac ggtgtgaaaa
     2761 gtgaaacttc tccttacagt gtatttaaat ggggagatac agcaggacag ggactttctt
     2821 atgtcgctgc taaagcaaat atcaaagtaa caaataaaga tggatctgtg gtattaaaag
     2881 aaaatacaga ttataaaatc caaaattcag aaaatggctt tgtgatcgat tttatcgtga
     2941 ataatggcca agtaagtgat actgtagcaa gtttacacgg acaagatctt caaatgactt
     3001 ataacatgta tgtaaatgac agcgcagctg ttgccaatcc attaacaaat agcgtggatt
     3061 tcgtttataa caacaatcca tttaatcagg aagaacacca cgaaaaaacg aaagctgacg
     3121 ttgtgactta tggtgcaaaa ttcttaaaag tcgatagcgg actatttgga acaggaatca
     3181 aagcaacacc gcttgaaggc gcagaatttg ctgctaaaaa cgcagaagga aaatattatg
     3241 gtggattagt tgatacggat aaagacggtg tcaaagaagc agtatgggta gacgatgtag
     3301 caaatgcagc gatcctaaaa tcagataaag aaggtcactt tgaaatcact ggtttaactg
     3361 aaggtgaata ttcactggaa gaaacaaaag cgcctgaaaa ctatcaaaaa ttaacgaaag
     3421 agatctcttt caaagtagac aaagattcat acaaagaaga aaaccgaatc acgatcaaaa
     3481 acaatcaaaa agcttccgta ccaatgaccg gtagcaacgg ttttcaaacg tatgtattaa
     3541 tctcttgctt gttgctagga gcaggggcac tttctgcagt tgtttacttt aagaagaaag
     3601 cctagaagga tcaaaaccaa tgattatcag gaagcctaag aaaaagcgaa tcatgggtaa
     3661 atggatcatt gctttctggt tactttcagc tgtaggtgtc ttacttttga tgcctgctga
     3721 agcgagcgtg gcgaaatacc aacaaaatca gcagattgcc gcaatcgatc gaacaggaac
     3781 ggcagcagaa acggattctt ctttggatgt tgctaagatt gagttaggcg atcctgttgg
     3841 tattcttaca attctaagta tttctttaaa actaccaatt tatgacggaa cgtccgacaa
     3901 aattttagaa aatggtgtag gtattacgga aggaactggt gatattacag gaggaaacgg
     3961 taaaaatcct ttaatcgcag gtcatagtgg actttataaa gataatcttt ttgatgattt
     4021 accttcagta aaaaagggag aaaagtttta tatcaaagtt gatggtgagc agcacgctta
     4081 tcaaatcgat cggattgagg aagtgcaaaa agacgaacta caaagaaact ttgtcactta
     4141 cttagaatca aatcccaatg aagatcgagt aacgctaatg acatgtacgc ctaaggggat
     4201 caacacacac cgttttctag tgtatgggaa gcgagtaacc tttacaaaat cagaattaaa
     4261 agatgaagaa aataagaaac aaaaattatc ttggaaatgg cttttaggaa gtactgtttt
     4321 tctttcagtc atgatcattg gttcgctatt tgtttataaa aagaaaaaat aaaggagggt
     4381 aaccaacatg agaaatagaa aatggctatt tgtttgtgca gccgctcttt tttctttcac
     4441 tgcctacaca cagttgacgt ttgcagacag acaagaaagc acggatagca cagaacaaac
     4501 aatccaaacg tcagatagca gcacgattca aagcagcgat gaaaccgtcg attcgtcaac
     4561 agttgattct tcagaagaac ctaaaattgc tgaagacgat caaatagtca aacagaccat
     4621 tcacgttcaa aagattattt cttcagaaca attagacaac caggggcatg aaaaacaaga
     4681 aggagtaaat ggcgcgaagt tttctgttta tgatgtatcg gacattttac aaaagatgga
     4741 tgttaaggat ctcactactg accaaattga atcccaatta aaaaatcgag tgaaaaaact
     4801 atccagtgat caattgaagc tagtttctaa tgaagaaaca aagacaattg atcaacaaac
     4861 aggtgtcttt gaattctcgg tagaagtaca ggcaaatcag aaacaagctt attatattgt
     4921 caatgaatca agtcctgaaa acatttctaa tagcgaggat attttgctat taacacctgt
     4981 ttctgataaa aacggtgaat tcttaaagga tgtgtggatc tatcctaaat cagaagcttc
     5041 gcaacctaaa gaagaagtaa aaaaaatcgt ttctactggc gtaaagaaaa acttctttga
     5101 gaattgttgg gattttgtta ctcacttgtt ttctagagat taaggaagaa ataaaatgaa
     5161 actgatcaaa ttattattat tcaatttttc ttgtttaacc gcagtctttt tgtactgcgg
     5221 tttattattt tctaaactat ctattttgcc aagctaccaa ctatacctaa tgaggatttt
     5281 aaacatacca gctgcttcca tgccgctaga tattatggaa aagctcatta ggaatccatt
     5341 atttactacg atcaatttga tcgttattgt cggaatttca ctagtcattt ataaaaaaga
     5401 ggggatgaag ggatgaattt taaacactcg tggacaagac ggttaaaata tgtttttccc
     5461 ataatgatga tgttaggtac attgtttggg ttaaaaacag ccaatgtctt tgctgaaaaa
     5521 gttattgtcg atccgtcaaa tccaatccag aacgtaaaga ataattttat tattccaaaa
     5581 gatgttccta atgcaaaaac aaatattact gtaaacggag cgtcaaaagt ttacaaatat
     5641 catgttgatg ccaacaaagg tggttttaca gacgactacg taggattagt ggagggaaac
     5701 aaaaatatac gctatccttc tttccaaaaa gaatacggtg aaacaaacct tgttttagaa
     5761 ggcgtaacaa ccaacaccaa agtcaatatt gactatggaa aaatcggtac gtataacggg
     5821 aaaaaagtca atatcaagct agttctatct aatattcact tatactcgga tacgttgccg
     5881 tggaatctac tagataacaa ctatacaaaa actcattttc gagacgatgg ctataaaaat
     5941 acgaatggtg caatgagtaa atctaaaaaa agaacagtac tttggatctc agacaatctt
     6001 ttctcaggaa ttgtttacca ttcaacacaa atgaatgtgc agctagtagc cacatatgag
     6061 gacggtagcc ccgttcaatt ttcaggagat acatttatca gttttaactc tttgaaccct
     6121 gcaggaggaa aaagcacgga tcttaaagga gaatatgccc actatgacaa aatgaatacg
     6181 acagattggt atgtcagaaa agatacggtt ctttcagaat ttaaatcttt ctacaacaat
     6241 ctgaacgtgg taggtggcca cccaggagga agctctaagc tgacacaagc ggataatgat
     6301 ttcaataatt tgcatgataa attaggagat ccgaagtttg gtcaaggtac tgtgtccttt
     6361 aaaatatctg aagcaaatcc gacgtttgtg attggttctt ctaatgttca aacctggttt
     6421 actttatctt ctgcgacaat ctttagtgtt gttcctgatc aacctgaaaa aacaggggtt
     6481 gataaaaatg gaaataacgt gaacgataaa atgttgcaag taggagacac gattcaatat
     6541 cgaatcaaac agaaggtgaa tcgattagga gtggatttac tagcggttta cgatcgattt
     6601 gagttaatcg ataatttacc aaaagaagta aattacgtcg atgcacacgt tgaaagtggt
     6661 acaaataaaa aattcgatgt atctggtgaa gtgacctatg acaaaacaaa acaccaggta
     6721 aaatatgcag cgaaagctga tacattaaaa aatcgaatga agtataacgg cgaaacttac
     6781 gaattagtga tcaatgtaaa agtcaatgaa ttggccaatc aaaatagtgt agccaaaaat
     6841 cagggaacgt cgatcattaa taaagtagaa aaggatacaa atatcattac cgtttatttc
     6901 cctaaaattc cagtgaaaga agtacaacaa aatggaaaag atgtaaacgg acgaaatgac
     6961 ggtaagaagg gtacgccgac agctccttta aacgcaggat ctgaagtaca atatctagtc
     7021 acacaaaaat ggcacacaaa aggcgtagac gctgcgtcag atcactacaa acaatttagt
     7081 attcaagatc caattgaagc tcgtttgaca tataaagaag gttctgcgca agtaatagat
     7141 aagagtaaag gaaaagatat tacttctgaa ggcacactaa cctatgatag caattcaaga
     7201 acgctgaagt gggaagcttc cgctgacttc ttaagtaaca atcttttaga tggacgagaa
     7261 attcagctga tatttacagc taaaactcca ttacaatcag aaaaaaatat tgataaccaa
     7321 gccgtagtag cagtagataa tgtttctaat aaaacaaatg ttgtgacaat tggggttgat
     7381 cctaacttgc cacaggtcat tgttcctaaa acaggttcta cacatttagt aacgatttca
     7441 gcggtcagct tactattact tgtgttagca acttttagtt atgcggttct gagatacatt
     7501 taaaataaaa aaacgaaaga atgaataaaa tgaaaaagtt aataatagta gttacttcct
     7561 tgattgccat gattgttatt ttagtcggat gtagtaatga aaaaaataaa caaactaatc
     7621 aagataatgg cgtattgaaa tcaggaacta tgtggaaaga agaagtaggt ggtcttgttt
     7681 ataatttgaa aattatagac gagactacct gggaatactc tgagagtgta tggcatccag
     7741 atcctgtaca aataacagtc aagaggcaaa aagactataa aggtttagaa agatacaaaa
     7801 tagtagattc agctggtgtt agggaattta taaacaaatc tgattcttta tttattgttg
     7861 ttccctatga gaagaatggc gtaaaaaaaa taatttttct tggatctagc aaggatgaaa
     7921 agcgaacgaa agagaaattg attcacgatg gatcacaatc caataaatat aagttacaaa
     7981 agactagcga gtgattgcta gtctttttat ttgtcctgaa ggaggaatca cagtggcaaa
     8041 agctaaaacg ccatttgaaa aagccgcgtc taaaatctta gaacgccgcg gtgtatctga
     8101 aacacagtgg aaacaagaag tccttgaaaa agcacaattt gacctattac ggggaaaaga
     8161 caaggactta gaaggatata tctatcaacg tgaacgtgaa aagcttgttt tcgatgaaat
     8221 tattaaacac taaaaggagc gaacaatttt atgtttgaat caattacaat gaatttttta
     8281 acacaaccga aagtagtagc tgcggttgat atttttagag cattacaaac tgccgcaact
     8341 agtttaaaaa acaatctgat gatttttgct gctgctgcgg ccgttgcttc cattgttatt
     8401 gggggtcttt tcttcctatt tggacgaaga ggagctgaaa atgggaagat gcacgtaggc
     8461 tatgtattga tgggggtagg agttatttta gcagcagctt ctattgtggt ttcgttattt
     8521 ggattgtttg gacaaacacc tcaaggatta taagaaagta aaaaagtagg agtaagaagt
     8581 agtcgatact ttttactcct tttttatgaa aggagaatac tcatgtactt tacgattgca
     8641 ggcaatacca aaacagataa agagttaata aaaaaagcaa cgaaggaagt aggaaagcgt
     8701 acttcttttt taccgaaaac aattaaatat tctgtcattg aaacggaaga acagctacat
     8761 gtgttaggga aagtactgga acacgaaagt cgatcttctg ttaagttgac tctcacgtta
     8821 catgacacag aaggaaaaat agagaaagct ggtacagaat acgtttttgg cccactggat
     8881 tatcaaattt cagaaggttt ctatcctaca ttgatggatt taatccgcct tgatgtgttt
     8941 aaagaaacgg atgaaactat gagtgtggaa gagtcttatc gaaacgaaaa acttttacaa
     9001 gttttagaag aagagttaga gagcgaccaa agaaaagtga atcaagggag tgaagtcgaa
     9061 gtaaatgaag aagtaccatg ggaacgacca atatttccaa agaaggagtc aaaagaaaaa
     9121 gagacagtct atgttgacga acaagctgat attccgactc agaaatcgat atttgaggaa
     9181 gagaacgatg tgatgggtca attggaggta gatccctttg aagaaacaat ggaggatgaa
     9241 gagacggccg aagtagaagt tgatccagtt gaagcgaatg aagagtcaac atggactgaa
     9301 ggggattctc aggaatatat tgaaccactt atcagtgaag aagtagaaaa aaacgaaata
     9361 gagatacaaa aggcaactgt atttgattat attttggatc aatatccgcc agagcatgaa
     9421 tggatgaaag aaaaaaacaa attattttta aatgagcttt attcgaaata tcaattaccc
     9481 caaacctatc aagcattttt agagagtaaa gaagaattaa tctcacaggg taaaggcaca
     9541 cttttagaac agttagagtc tgtccaatca acaaattggc atgagcttgc acaagaagat
     9601 ttattagcca cctttgaaga acgtcaacaa gaaacagatc aagaaattaa tgagtatgcg
     9661 acggaacagt cgtctaattg ggagaaagca aaagaggaac taaataaaga agaacagcga
     9721 gtgattgaag aagaagtaca aaaaattaaa gagcgatacg aaaagaaacg cgacaatgcg
     9781 tattccttgt gtgtagcaaa gatcaataat tttactcaga cgaatcaaga agcgttatcg
     9841 aaagaaaaag aaaacttgtt aaaagaacgt attaaagaac gaaaagaaga attttaccga
     9901 ggtttgcgta cagataagct aaagattagc aaagaattaa atgaacaact gaataagcta
     9961 tatcaaacaa cgactgaagt tcttatggaa aaatacgaaa agatccagca agaattacaa
    10021 gtgcaaattc caaaatggaa aaaagagtat gaaattcaac aacaagaatt gaaagaacaa
    10081 gaagaaaaac aacggcaaaa agaaaaagag cgcgaacaat tagaactaaa aaaacgtgaa
    10141 caggatcttg cggaacaaca aatggcaata gaaaaacaac ggttaaacca agaaaaggaa
    10201 aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg atacattgcg acaagctcag
    10261 ttaaattatc tagcacaacc tcaaccaacg ccttcagtag ctgctgttca aaccccaatg
    10321 aacaaccaaa caaatctaac tccttcacca cagcgttcct ttaacggatg gatggtggga
    10381 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
    10441 aatcaatctt cacaggctga ttctgtggcc acaacgacgt caatcaagga agaagcccaa
    10501 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
    10561 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg attctagtaa ggctaaagaa
    10621 ggatcaagcg attcaactgt acaatcaaac acttccgaaa gttcagaatc aaatgaggtg
    10681 aaataatgcg tgttagagaa acaacgaagc gaaaagacaa acgattcaat ttagaaaaga
    10741 ataggtatct ttctcatttc tatcgctata ctccctcagg agaagagcta ccagaaaaac
    10801 atgacaaacg catttcgaaa aagatttttt atagtggact tgtatacctc cctttagagt
    10861 atttagtaac aagagtacca gaagttttca accgattgga aattgttaag ggaagcttac
    10921 aaggaataaa tctgtttcaa ttacaggaaa tgccacaggt ctttcatttt gattcagctg
    10981 gtttttggaa tccatttact cctacaacta gtttgggtac acgtttagca atttcgacag
    11041 tttgtgtggt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtcctctag
    11101 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
    11161 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
    11221 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
    11281 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
    11341 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
    11401 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
    11461 tgagtgataa tccattggca ctcatcgtga aatactggaa acgaggagac atagatactg
    11521 caacacagtt aaccaatact ttcacaaata cgatttatta tgatgccaac gcttcagata
    11581 acaaatactt taacgaaaac gcgcagaaag ctgtcaatgc gcttattttt gcgttgttag
    11641 aacaagctga tcaaacagga gattactcca aattgacacc gaataatctc gttgagcttc
    11701 tttctgagat tggcggtttt aattattatc cagatccaga caatgaattc aaacagttga
    11761 atgccttaga tgaatttatg aatcagttgc cacctggaaa cattgcaaaa aaacaatatg
    11821 gggcaacgaa aattggatct gataaagcga agggaaatat tttatcaacg gcaatcacgg
    11881 gattgaatcc atttactcta acgaaaattg caaagatgac ttctcaatca acgattgatt
    11941 ataaatcggt tgggtttcca aaatacttag atttgaaatt acatgaaagt cttttaaatc
    12001 aacgagtcgt agtagaattt tatcataagg gaaagaaatt tcatgaagaa cgtgtcaaag
    12061 taggctataa aggattttgt gaaatcaatt ttgattgtca tttagttgca ggtgatcaag
    12121 tttgtttaaa atacgatcat caaggaaaag aatatgtggc ttggtatgaa ttttctcaaa
    12181 gagtattgaa agacgaagag ggtaatatcg tttatcggaa aaaacgagga gaaacccatt
    12241 tgccagaatt agaaaaagaa gccgttctaa cattagatga ggagaaaagt aacttattag
    12301 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg cttacacctg
    12361 attatgatcc atctaaccat atcattgtta gtatttttct tagtcagtta tataaagaat
    12421 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgcaggatt cattttattt
    12481 tagatgaatt tgggaatatg ccaccaatgg ataatatgga aggaatcatg acggtcacag
    12541 ctggccgaaa catgttgtgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
    12601 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
    12661 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
    12721 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
    12781 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggtgaa acgattgtac
    12841 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
    12901 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
    12961 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
    13021 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
    13081 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatctcttg
    13141 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca gcagatcgac
    13201 ttcaagaata tgttgaaaag ttgatcaaaa ataaagaaat ttctgaaatg attgggaaac
    13261 ggttgttatt ggaatctgaa aaggtggtga aagaatgatc attggaggaa ttttttctgg
    13321 catagccgac tggtggtcag gcttattcgg agaaggggat gcaaacccca aaaccgtaac
    13381 gaaagtctta caagattatg gtaattatct gcagtatcaa gatgtattta gttggatttg
    13441 gaacactttt ctgtggggat tgattaaagg attatataat ctcaatactt tactagaaaa
    13501 aaccatttat caaagctttg atttaaaaga cttattgaac gccgctgggg taaatgcact
    13561 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
    13621 aggcttgaaa ttttttatga gtaaacaccc accaaaagtc aaaaatatct tggttaattt
    13681 atttatggcc atgatcttga tttttggtgg gagttcttta atcgatgaag ggttaaatat
    13741 cagtaaaagc ttttacggag acgtcactgc agcaaataaa gcagataaaa cgggttcacc
    13801 tgcttttcaa attattcaaa ataatgtgat cgatgtcagt acattactag caactgaccc
    13861 gaataaagta gaatccctgc caacagacaa aagaaatgct ttaacagcga aaaattttaa
    13921 aacagcgaat atcaatggga ttattactcc taaacaagct aaagaaatgg cggataaaga
    13981 taagagtggt ttatcagatt tacaaaagga tcggttaaga gcgttacaat accgattgga
    14041 actagatgat aatgggaaag aaattcctgt tcaaattagt gacgaaggct tagcaaaata
    14101 tgtctatact tcagggtatc gtcgatacag tagtaaacca ggtattatct tagctggtga
    14161 atcttctttg gcggtggcct atttatttat cctatttacg atcgttactt gcattattga
    14221 gctggtattt aaaaaattct atttagtgat tgccgcgtca acggactttg agacaggcca
    14281 acggatgaaa agtgcgatag aagatatttc tcaatccttc ttgttattgg cgtttacgat
    14341 tttagaatta cgaatctata cattaatgct ttcaggtatc ggtgatttgc atgcggcagg
    14401 aaagatcaat ggattcttgt atgttgttgc tttaattgtg ctaactattg cgctattcaa
    14461 aggctctcaa tcggttacga agattttcgg tgttgatacg agtctgaaaa acggcagtaa
    14521 ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa
    14581 aaaccttgca agtgctggta agagtggttt aagccattta aatgatattc gtaaaaatgg
    14641 atttggccat cgaaaagatg ttacccctga taacggggaa gaacaatcag acgataaagc
    14701 tacaactgta cctaatagta agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg
    14761 tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc
    14821 tgaagatctt acagaaaaag ccaaagacac cttagaagag aaattagacg gcacgagagc
    14881 taaagaattg gctgaagctg tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa
    14941 agcttcagaa gtcaaagaag gactaaaaga tgcgtatggt gaaggacaag ttaatgcagc
    15001 agtaaaagct aatacaacaa ccgaaaaaga aaatcaagaa ggtatagtga ttccttcagt
    15061 caaagatcac caagaagcgt tgcactctaa tgaaaattcg attgaagaga aaaattccat
    15121 agaagaaaga aattcagaag aaggaaaaaa tggacagtta ccaaaaactg atccagaaca
    15181 atatcgctat aaacctaaca gtgaccttga aaatgagaag cgaatttcag atttatcaaa
    15241 agtaaatcaa gaagacaaag cagaaaatga gggacagtta ccaaaaactg atccagaaca
    15301 atatcggtat agatctgtta actccgaaaa tgaaaaacgc atttcagatt taccaaaacg
    15361 aaatcaagaa gtacaaacgg atgaggattc catcgttctg ccaaatgata atggaatcga
    15421 agttggaact gaaaaatcaa cgaaaattgg tttacctaag gcacaaaatt taccaatgaa
    15481 agatagtcgt ttgaatcaat tcaaggagaa tgatcaaagc tttgttcacc aatcaactgc
    15541 aggtactgtt caaatgaaac caagtcgttt gcctgaaaat ggaaatctga caactgtttc
    15601 ttacaattta tctgaaagtg gtgagacagg gaatgcgtcc gttcaaaatg ttgtgacaag
    15661 agaagctgca ccaaaaccta ttcaaaacgt tcatacaggt tacaagccag caaatgtggc
    15721 atttactgca ccaacaaaaa taaatgtgac aagagaaact aaaaacagga acaatgcatt
    15781 gaaagaacaa ctattgaggg atatcaaaca ggatatcaaa ccataaaagg cctacaagtt
    15841 aggcctttta tttatatgaa aaggagaaat caattatgga taaatatata aacatgaaaa
    15901 accctccaag atttagtgga gaatggaagt ttttcgcttt tttcttttta gatctattaa
    15961 cgcttggtgg ctgctttttt tgggcgaaaa tggtcaataa tcttgtccac ttaccactct
    16021 tttttgcact cttgaattat ggcttatgga ttgcgatagc gttagtgatt gtttggcgac
    16081 cctataataa cccaggtcaa cgacaaattt ttgcgattat cgagtctatt ttttttgcgg
    16141 acaagaacca ttatcatgcg ttagatccta accgaaatta tcatggtacg tataaggagg
    16201 atgaataaat gcgagtcaaa ggacaaaatt cagttgaaag gaaatccatt ttgcagtttg
    16261 gaaaaccaga agagcgctct atcttggact tatttccgta taaatcctgt acagaagagg
    16321 gagtcttaat tactagagaa gatcattttc aacgttatta tcgtgtagtt tctacagacg
    16381 tggaaggatt aaatgaacaa gagaaactag aacgaatgaa tcagttgacc accgtcatgc
    16441 gtacgtatgt gccaacaatt aagttagtta gtttgaccac agagacggat ctaacggaac
    16501 aaatcgtgca aaagcggcaa ttattagata aaaatcgtat ggaacagtcc ttaggaagaa
    16561 atctacaacg attaaaaaaa tatgaaaaga aattagtcga agaaatccag gaattgaaac
    16621 gagcagaaca agaacgtcct gacttaagct tctttttcgt cattgaagga aagacaatca
    16681 aggatttaca aaccagaaac cgtcagttaa tgcgatctag tggcatttta gggttaaaac
    16741 cactctacaa aaaagaattg atcaagattc tgtatcgaat gaacaatatg aatgatgagt
    16801 aggaatggag aagagagaat gaaatatccg ttgatatggt tagaaaaaga tgttccgaag
    16861 gaagagacca ctagaaaatt tcctcacact ttaagcctgg gtaagttggg aaaagggcaa
    16921 tcttttaacc aagtaaagaa tcaaacaaga agaggtgact gtacatgtta gaagcaatta
    16981 aaacagcttt ttcaccacaa aatgatcaaa aagtacttga tccattaaag caactaggaa
    17041 atcgggaaat agaaatttta aaatccaaag ggtatgattt agatttttta aaacaagtga
    17101 tgccacaagg tgggttaaat tttgatgaag aatatgtgac gtatggtaat ggttattcac
    17161 gatgtgtgac agtttatcac tacacaaaaa atcctacgct tttttggttg gccaatctca
    17221 tgaacaatcc tcatacagtg gccacactcg atgttagcac agatgaaaag caaaaagtga
    17281 ttgatcaaat caatcgtgcc ttagatgaat taaatgatcg atcagataat gaacgaaaag
    17341 cgactgaccg aaataaaagc gcagaagaat acctggaact agcggaatat gctcgtaaat
    17401 tgacttctaa tggggaaatt tctaagcaag tcaaagtgag agtacatgtt tacggaaaca
    17461 cgattcaaga agtagatgaa cgagcgcagg aactgatcga aaatctaaaa gggatggatt
    17521 atcgtgcggt gacttatctg cataaaacca aacgagattg gcaatcgatg tttaccatgt
    17581 tcgatgaaca agctgaatgg ttcggaaata tcaaagggca atcactacca tcgttaaaca
    17641 ttggcggagg tttcccgttc caccaccaaa cgctaaaaga tccaggaggg acacaatatg
    17701 gggaaaccat gacaggcggc ccatttgtct ggaatgcgac gtataaaaat agtgtacggt
    17761 tgtctactaa taccttgctt ctaggaatga tgggagctgg taaatcgacc gttcttaaaa
    17821 tgatcgcgga agcccattta gctgcaggag actttatttg gggctttgaa aagggaaaag
    17881 actttattcc tttcttaaag gaatataacg gtattatggt gcgtttagac ggatctgacg
    17941 gaatgatcaa tcctttagag atatttgcca cacggacgta tgatgaagca tccagtttat
    18001 atgacgatgg atcagtcaaa gatttgaaga tcaatgaagc agcgtcctat caaacacact
    18061 tagacaaagt ggtctatcag gtacagttag tgagtcctca gctaaaagga acgatgaaag
    18121 cagaatttaa gacctactta aatcgttttt atgaagagta tggaacagta ccacgcggct
    18181 ttacttcctc aaattcgcgt tcaaatacgg agacgcaagt cacaggtaaa gatccagaag
    18241 cttatccgac gttcaaagaa tttttagact ttttaggaca actggaatta ccaggtgctt
    18301 cccaagagaa aaagaatcga aaagaagaaa tggaatccat cgtagaatcc ttatgtgaga
    18361 cgtatggcat gatttttgat gggcatagta cgattcgaca ccttgaccaa caacagttgg
    18421 tcttttttga cattgactct attacaggct tagatcgagg ggtacaacaa tgtcaaatgt
    18481 acatggctat gacgttgatt tggaatcagg cattgattca aggaagaaga caacgacgtt
    18541 taattaaaga aaaacaaatg gaagaacaag acttcaaacg cttcttggcg atgattgatg
    18601 agtgccataa tgcgattaat ccagatttta ttgaaacggt aaattatgtc acaaactttc
    18661 aacgagaaat gcgtaaagta gaagcaatga ctgttttagc tacacaatcc ccacaagaaa
    18721 tggtgccaga aaatatgtct tctgataact tctcgaaact gaagaaagtc tttgaatttt
    18781 cttctacgaa gatctttatg cgtatggatg aatctatttt acgtcatata gaaggattag
    18841 tgggtaaatc aatgacgaac tcagaattag aatccattcc tcaacaaaat caaggagacg
    18901 ctgtgatcaa ttttggctca aaagaaacca ttcgtgttca ttttagtccg aacaaacgtc
    18961 aattaaaatt gtttgatgga ggaaaataat ggaaaaccaa aatgaaagtt taatcaaaca
    19021 gtatgttaag cgtcgtgcaa agcggcgctt atttttgtgg gtgtttggta cttctgcagg
    19081 tttgattact atattgatca ctgtgtttgt gacccttttt ctaatacttg cggcaggttc
    19141 tattgataat tctgattcaa gtagtggtgg cgaagctttt acaggagaat attcggaagg
    19201 cttacccatt tataaagaaa tcaaaggaag aggccctttt tctgatgaga ttgcgcaata
    19261 tgctgtaggt gcagcagtga agtataaatt attaccaagt gtgatccttt cgcaatacgg
    19321 ttatgaatca gcgtttggta cgtcggcgtc cgcaagaaat gatctcaatt attttgggat
    19381 tacgtggttt gatgggtgtc tgtttcctaa aggaactgcg cgtggaattg gtggcattga
    19441 aggtggctgg tacatgaagt ttcccaactc aaaagctgcc ttctcctatt acggctttat
    19501 ggtagccaca caatcaaatt ttaatgcttg tgtcggaaat aaaagccctg gcgcaagctt
    19561 attgatctta ggacgaggag ggtatgccgc cgcaggaatt actgaagaca gtgcctatta
    19621 taaaaattgt atgtctatta ttaatacaaa taaattgatg gagtacgatg agtttgcgat
    19681 taaacactgg agagaaggcg gcggaactgg tggtacgatt acaggaagct ggggcaatcc
    19741 attcccagga agtagcttag ataaaaattc gttcagtgga ggacaacttt ttgggaaaaa
    19801 tccaggagga gaattccgac ctaacgggtt ccatgatgga ctagactttg gttcagtcga
    19861 tcatccagga agtgagattc atgcggttca tggcggaaaa gtcgtgtacg ttggcaatcc
    19921 tggtatttca ggtttgggtg cgtgtgtgat cgtgattaat tacgatggct tgaatatggt
    19981 gtatcaagaa tttgcaaata gtacaggaaa ttctcgagtg aaagtaggcg atcaagtgaa
    20041 agttggtcaa gtaattggga tacgagatac agcacacttg catttaggat ttactcgaat
    20101 ggattggcgt caagcacaag gacatgcatt cattgatgat ggtacatgga tcgatccgtt
    20161 accatttttg aatagcagca aaaaatagat caggagaaaa aatatggaaa ataaaacctt
    20221 aaaaatcgtc ttaacagctg caattagcgt tagtttaatc ggcaatattg cgttgttttg
    20281 gcagcttaaa gaagaacaag agacaaatac ctataaaatt gaagaagtgg ctaaaaacgc
    20341 gtctgataag ctcaaaaaaa attcttctga ggcacaggcg aagatcgaca agttaaatca
    20401 aaccatcaat gagaaaaacc aagaaatcga tcagctgaag caacaagatg gcacaaaagc
    20461 aaaagaggaa gtaactgaag cacaaagaaa gacggcagaa gagttcggtc aattggccat
    20521 tgataaatca ttaagcagaa atgaattaac ggaaaaacta aaagatgtcg caacccaaga
    20581 agtgatcgac aaattaagtc ctgcagacga tgatcaccaa catgacgact acggaagtta
    20641 ctcttttaca ttaggtgacg tgcaaactta cgctcaaaca agcagtgtaa aagaggaaca
    20701 aaattttgtc gtatttgttg attatacaat cggcaatcct caatttaaag atgtcaaacc
    20761 acaaaaaata aaaggtggat taactgtcac tgaaaaacaa gtagatggtc aatggaaagt
    20821 cacagacttt agttatttca ctcgttagga ggaagaaaat gagtaagaaa aaaggagcaa
    20881 gacaacgttt taaaaaacga aaaagttatc ttagatggct ggggatcttt atgattctcg
    20941 gctttttgat tcttgctagt ccgatttaca cacataaaaa ggtagacata ccagatgaag
    21001 taagcacgaa cgcattttta aacacgaagg aactgacgat ggtttcaaaa gaaaaaggag
    21061 cgaaccagca attagttttt gaatttgcga tcgacgatga agatcagagc ttattaaaag
    21121 aactagctaa tcttaattat cgtgtagaag taaagacagc taaaggggat tatcaagcaa
    21181 taaaagccaa agtgatcaag gtgtctgatg attattttgt agtgaagtta gcacatgtac
    21241 cagaagaata tatcgccatg cgtttaacca ttattcctga aaaaattgat ccaaaagtgg
    21301 atatgcaaga accacaagat ctgatttatt acgttcacga agataaggtt aaagatcaag
    21361 tgaaagatga agattatgaa caacatgcga tcaattacaa agtgaaagga tacaaaaaag
    21421 aacaaaaagc agcacaaaaa caaatggaat ccttccaagc aaccattgat ttgaatgaag
    21481 aattagtgaa gaagctaaaa gatcaattgc cttatcaagt cgcagatgat caggaaaata
    21541 ctgaaaacaa gattaatagc taccagcaag aaattgaaac atcgaaaacg caaatgaaag
    21601 atcaagaaga gatcattcaa aaacttcaag aaaaaattga tcgtatgaca aaagaaaaga
    21661 tttagaagga caggtggagt gaaaaaatga agcgaacaaa cttggctgac aaagatactt
    21721 tcgctgagca gaaagtattg attaaagcct atctcaaagt tgtaaaagct aaagaaatat
    21781 gtcgagaagt gaatttatct aatacaacaa tatctgagtt gaagaatggt cgaaagaatt
    21841 tagatacttt aaaattcaga tcatttgaag ctctatatgt agctgctaaa aaatatttta
    21901 cagctgataa caaataaaaa acaggagtat tttatgtgaa aaaagtatat aaaaaatgct
    21961 tatgaatcag ctatatcacc ttaaccattt gttaatgaga tgggagaaaa cattaaccac
    22021 gattctaacg caaacatcaa gcacataaaa catttaacac tcctcctttc tggctgaaga
    22081 tttcaaccaa acaggaagac agcgtatcaa ttatagcata cactttttct taaattcatt
    22141 tttcatatga caaaatattt ccttctaatc gacaaagtta gaaggatttt ttattgtcta
    22201 aatacacaaa taaccttaat ttataaagag cataaggaaa cgtgggaaca ctttttaaaa
    22261 aaagtgttcc aataggctgt gcctattacg tttctcaaaa ataattgtgg gaacaaaata
    22321 tttaaaaata ggatagtgaa aggatagcaa aggatagtaa aaggatagaa gcaggatagc
    22381 gaaaaggata ggctcctttt cgctatcctt tttaaattgg aggaattaat atggaagtaa
    22441 gaataagaaa tgtagatgag aaaatcgtaa gagaaattga tcgaagatgt gacgaactaa
    22501 gtaaccgaac gggtaataaa tggagtcgta acgattattt aaagctactc gttgaaaatg
    22561 attttgatcg tcctttgatg gaatataaac aagataaatt tgatcaatta ttaaatcagt
    22621 taatagataa attttctttt gttcaaagtc ataatacaaa agtattagaa gaatatattt
    22681 gtcagaataa tcaaattatc gaacttttaa ttgaacaaag tagaggagga gaagtttaac
    22741 atggccaaag ttatagacaa gcatatttat ttttatgaaa cacatataga tgttctagat
    22801 gaaattattg caactcatgc agaggttaaa aactatagtg aagccgttcg gtttctatgt
    22861 atgaattatg attcagaagt ggagaaaaaa gtaaattcca atcaagcaaa gttgaatgca
    22921 atgggaaaag aaatttctat tttaaccgaa atggtttcag agtttgcgga tctccatttg
    22981 aaagatacag ggattttaat tggtacaaat tcaactgttt accaagaagc aaaagagcgt
    23041 gtggaaggca agataaaagc aaatcaaacg aagaaatatt ctcctaagat aaagaaaaca
    23101 ataagcgaat cagtgactac aaaaagtgaa gtatttgatc caacaaggtt cttaaaacga
    23161 taaaaattag aaggagaatg tgatgagtaa atcacctgca gtcattctca cttgtcaatt
    23221 taccttgccc aataaaaaat ctttttcaac atacattgat tatatgacaa gagaaaaagc
    23281 gctggaagaa aaggaacgac gtacgcccga ggaagaacag gaactgcgtc taatcaagaa
    23341 tgctttagag gatttttata ttcctgaagg agaaactttt tcggagagta agacgggtaa
    23401 agaattcagc gaaacgtcga atgaagcgca atcgttgatg aaacatggaa tcaattttga
    23461 caagttagaa gaacaagatt ttaccaaata tctttcttat atgacacgtc actatgcgtt
    23521 agaacaaaag aaagtactga cgcatagtga acaaaaagaa taccaacgct tgaaccaagc
    23581 aataaaaaaa tatcaaacag agccacacaa aaaagcttca ggaaatcttc ctggagtttt
    23641 ttctatggct tcagacgaag tgaaaggaag tgatctgaag gagatcaagc aaaccttcca
    23701 aagaggacaa caaaaaggct caatcattta tcaagatgtt gtctctcatg atcattcgta
    23761 tcttgaaaaa ataggattgt acgatcccaa aacagatact ttagatgaag aaggtttaaa
    23821 agcagctgga agaaaaatga tggagacatt gttcgagaaa gagcaactga atgaaacagg
    23881 ctactggatg gcaacgattc accgaaatac caaacatatc catattcatt ttgcagttgt
    23941 tgaaaaaaaa aatacgcgta aaccatacac tgagattgaa aatggtgtga gttatatcgt
    24001 accgaaaggg aagagaaggc aagcaacatt agacgaaatg aagactaatt atgtacatga
    24061 gttagaacgt ttttcttatg aaaaaaatcg aatgggtatg gagaaacgaa atttattgac
    24121 acggaaaagt gatttgcgta acaccttaac caaagtagtg aaagagccag tacgttatga
    24181 tcgacacgca atgagcttat taactgaagt atataaaaac ttacctaaaa agcgttcgga
    24241 atggcattac ggggatgaaa atcgaaccaa actttcacca tacacaagag agaaactcga
    24301 tgagttaacc caatatgtgt taacaaaaga acctggatat caagaatata tacagatttc
    24361 aaaagcattg aaagaagaag caaaaagtct ttatggggaa agcaagagag aatcaaagga
    24421 tgcagaaaaa aatgctctct ttgatttaaa aaagcgcaca ggaaatgcca ttctaacaga
    24481 actgaagaaa caggatagtc aaataaaacc tttaatagaa aggatggatc agatgcaatc
    24541 tatttacaat gaacgatcct ttcgaacact taaacagttg ccaacgaatg aatacaaata
    24601 tagttacgaa gaattcatga agcgcaggaa acaattccaa caaagagttg tcacgcaacg
    24661 gagtttgcgt caattaaatc gaacgattaa tgcccatgaa gaaaaatacc acgcaagtaa
    24721 agcatacgaa gagcaacaac gaagaattca aatggaacaa gaaagacagt aggaggaata
    24781 gcaatgaaac aatatttaga tcaatggaaa gttatagaag gatcattaag agaagaacga
    24841 atcgaacaac ttcccgattg tttagaaaaa gagcatttgt ttcagataag agaaatgttg
    24901 ataaacgaac agtttgatcc aaatcaattt ttagtcgttg agtacccagc tactggtgtg
    24961 tactgttgta atcatgtgaa gggtgaaaaa tattttatca ttcaagaata tgagggcaag
    25021 ttagcgcctt attatactac ttgggaaatg aatgaagagg ggatcaataa cttcccgtgt
    25081 aaaagcattg aagagtctat ttcactcaca gaatgttagt tgttaagtga ataaaagaag
    25141 taaaagagtc ctgaacgtga ttgtccagga ctcttttttt tcactgaatt gttttccgcc
    25201 aattcattga gtggtgcgat ttccgtctac attatatcaa aagaaaggag gaagaagaaa
    25261 tggcttattc aacgattgga gttgttgttg aaaaaagcag agacaatctt gtttttgtga
    25321 cagaaattca aacaggcaga gcttttgtag tcacagacaa agcggcaaaa gcatatcaaa
    25381 gtggcgatat tttaacgtta aatctgacta cgaagacgtt cgtcgatgcg gcagaagatt
    25441 atccatt
//
LOCUS       DAZKOR010000036        25358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000036 DAZKOR010000000
VERSION     DAZKOR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.036"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACG8ZM_001381"
     CDS             complement(132..509)
                     /locus_tag="ACG8ZM_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492346.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACG8ZM_001382"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACG8ZM_001382"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0492347.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACG8ZM_001383"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACG8ZM_001383"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0492348.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8ZM_001384"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACG8ZM_001384"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0492349.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACG8ZM_001385"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACG8ZM_001385"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0492350.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACG8ZM_001386"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACG8ZM_001386"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0492351.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACG8ZM_001387"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACG8ZM_001387"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0492352.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACG8ZM_001388"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACG8ZM_001388"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0492353.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACG8ZM_001389"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACG8ZM_001389"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0492354.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACG8ZM_001390"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACG8ZM_001390"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0492355.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACG8ZM_001391"
     CDS             10661..11575
                     /locus_tag="ACG8ZM_001391"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0492356.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACG8ZM_001392"
     CDS             complement(11708..13273)
                     /locus_tag="ACG8ZM_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0492357.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACG8ZM_001393"
     CDS             complement(13266..14717)
                     /locus_tag="ACG8ZM_001393"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0492358.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACG8ZM_001394"
     CDS             complement(14731..15747)
                     /locus_tag="ACG8ZM_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0492359.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACG8ZM_001395"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACG8ZM_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0492360.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG8ZM_001396"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACG8ZM_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0492361.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG8ZM_001397"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACG8ZM_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0492362.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACG8ZM_001398"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACG8ZM_001398"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0492363.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACG8ZM_001399"
     CDS             21340..22764
                     /locus_tag="ACG8ZM_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0492364.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACG8ZM_001400"
     CDS             complement(22806..23063)
                     /locus_tag="ACG8ZM_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492365.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG8ZM_001401"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACG8ZM_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0492366.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACG8ZM_001402"
     CDS             24337..25314
                     /locus_tag="ACG8ZM_001402"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0492367.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8202 a         4317 c         5695 g         7144 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DAZKOR010000037        24970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000037 DAZKOR010000000
VERSION     DAZKOR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.037"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..519
                     /locus_tag="ACG8ZM_001403"
     CDS             <1..519
                     /locus_tag="ACG8ZM_001403"
                     /inference="COORDINATES: protein motif:HMM:NF028094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0492368.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESSTTVSDANILANIILDFIFEHDIPFKNGYEILPQNEQYFFYKCLTNRVCCSCGKK
                     NADIHHVDSVGMGNNRKKINNSGRRFMALCRECHTKIHVEGFTTFTTKRKLTAVVLKD
                     SDLKRLGLNIIE"
     gene            512..1042
                     /locus_tag="ACG8ZM_001404"
     CDS             512..1042
                     /locus_tag="ACG8ZM_001404"
                     /inference="COORDINATES: protein motif:HMM:NF019106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HFQ0492369.1"
                     /translation="MNELWMDIEGYEGVYQVSSLGRVKSNRPNYVINKGYVNKEGFIM
                     KPSDNGKGYQIIFLSGKGFRDRRYVHRLVAFHFLKEAYFEGAEVNHKNGDKSNNTVEN
                     LEWLSSDDNKKHARKNGLTNLKGPSKLTDLQALAIKRLYTNKLMSSGEIMKLFNVSRH
                     TVLNIASGKTFSFLED"
     gene            1147..1977
                     /locus_tag="ACG8ZM_001405"
     CDS             1147..1977
                     /locus_tag="ACG8ZM_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0492370.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1994..2845
                     /locus_tag="ACG8ZM_001406"
     CDS             1994..2845
                     /locus_tag="ACG8ZM_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0492371.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2842..3201
                     /locus_tag="ACG8ZM_001407"
     CDS             2842..3201
                     /locus_tag="ACG8ZM_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFQ0492372.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            3216..3377
                     /locus_tag="ACG8ZM_001408"
     CDS             3216..3377
                     /locus_tag="ACG8ZM_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0492373.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            3374..3679
                     /locus_tag="ACG8ZM_001409"
     CDS             3374..3679
                     /locus_tag="ACG8ZM_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492374.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            3679..3996
                     /locus_tag="ACG8ZM_001410"
     CDS             3679..3996
                     /locus_tag="ACG8ZM_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFQ0492375.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVKKYKEILDKYME
                     DSK"
     gene            3993..4388
                     /locus_tag="ACG8ZM_001411"
     CDS             3993..4388
                     /locus_tag="ACG8ZM_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFQ0492376.1"
                     /translation="MILKYRAWNKKTQSFIDYGDLVLDLRSGKIYAGDIGLVESTIDV
                     TDQIELMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLGH
                     RNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            4385..4723
                     /locus_tag="ACG8ZM_001412"
     CDS             4385..4723
                     /locus_tag="ACG8ZM_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492377.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            4720..5088
                     /locus_tag="ACG8ZM_001413"
     CDS             4720..5088
                     /locus_tag="ACG8ZM_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492378.1"
                     /translation="MNEEIKKSIRYIDRALSQLEPLRIESHKGMSVYPNQYDKMNADL
                     RNAKLALINIKNNKGCQYCKHSEPLNDTSNSNFATAIECEENGYYIATEYDDGDYADN
                     GYSAYIQYCPMCGRKLEVAE"
     gene            5085..5390
                     /locus_tag="ACG8ZM_001414"
     CDS             5085..5390
                     /locus_tag="ACG8ZM_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492379.1"
                     /translation="MNVQVYLKRNLARFPRSWDTNSHPSEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            5414..5617
                     /locus_tag="ACG8ZM_001415"
     CDS             5414..5617
                     /locus_tag="ACG8ZM_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFQ0492380.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            5614..5910
                     /locus_tag="ACG8ZM_001416"
     CDS             5614..5910
                     /locus_tag="ACG8ZM_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492381.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMQTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKENLAVVRLVDIELINDK"
     gene            5986..6402
                     /locus_tag="ACG8ZM_001417"
     CDS             5986..6402
                     /locus_tag="ACG8ZM_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0492382.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLIQYR"
     gene            7117..7284
                     /locus_tag="ACG8ZM_001418"
     CDS             7117..7284
                     /locus_tag="ACG8ZM_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492383.1"
                     /translation="MYRPQYLEQKHEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKY"
     gene            7310..7654
                     /locus_tag="ACG8ZM_001419"
     CDS             7310..7654
                     /locus_tag="ACG8ZM_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0492384.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            7659..7940
                     /locus_tag="ACG8ZM_001420"
     CDS             7659..7940
                     /locus_tag="ACG8ZM_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492385.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            8043..8357
                     /locus_tag="ACG8ZM_001421"
     CDS             8043..8357
                     /locus_tag="ACG8ZM_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0492386.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            8335..10029
                     /locus_tag="ACG8ZM_001422"
     CDS             8335..10029
                     /locus_tag="ACG8ZM_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0492387.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKNR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            10049..11227
                     /locus_tag="ACG8ZM_001423"
     CDS             10049..11227
                     /locus_tag="ACG8ZM_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0492388.1"
                     /translation="MGIFQKAVGYFTKKATVTLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            11190..11876
                     /locus_tag="ACG8ZM_001424"
     CDS             11190..11876
                     /locus_tag="ACG8ZM_001424"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFQ0492389.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            11876..13036
                     /locus_tag="ACG8ZM_001425"
     CDS             11876..13036
                     /locus_tag="ACG8ZM_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0492390.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            13046..13921
                     /locus_tag="ACG8ZM_001426"
     CDS             13046..13921
                     /locus_tag="ACG8ZM_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492391.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSNVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            13918..14229
                     /locus_tag="ACG8ZM_001427"
     CDS             13918..14229
                     /locus_tag="ACG8ZM_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492392.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            14219..14572
                     /locus_tag="ACG8ZM_001428"
     CDS             14219..14572
                     /locus_tag="ACG8ZM_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0492393.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            14562..14963
                     /locus_tag="ACG8ZM_001429"
     CDS             14562..14963
                     /locus_tag="ACG8ZM_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492394.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            14956..15360
                     /locus_tag="ACG8ZM_001430"
     CDS             14956..15360
                     /locus_tag="ACG8ZM_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492395.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            15372..15980
                     /locus_tag="ACG8ZM_001431"
     CDS             15372..15980
                     /locus_tag="ACG8ZM_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0492396.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIVTNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            16000..16362
                     /locus_tag="ACG8ZM_001432"
     CDS             16000..16362
                     /locus_tag="ACG8ZM_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492397.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            16365..16547
                     /locus_tag="ACG8ZM_001433"
     CDS             16365..16547
                     /locus_tag="ACG8ZM_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492398.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            16565..19897
                     /locus_tag="ACG8ZM_001434"
     CDS             16565..19897
                     /locus_tag="ACG8ZM_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0492399.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFIQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGV
                     GEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMK
                     QTTQQIIDSITKIQNDNASAWR"
     gene            19948..20685
                     /locus_tag="ACG8ZM_001435"
     CDS             19948..20685
                     /locus_tag="ACG8ZM_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0492400.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            20695..22986
                     /locus_tag="ACG8ZM_001436"
     CDS             20695..22986
                     /locus_tag="ACG8ZM_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0492401.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYINDGDGNSLGIDGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            23010..>24970
                     /locus_tag="ACG8ZM_001437"
     CDS             23010..>24970
                     /locus_tag="ACG8ZM_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492402.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDDQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEIKGRMDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPNLMSKLKSSDFN
                     VGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRFYISVSFSN
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSAY
                     EIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLTF
                     TPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSNN
                     PKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAKD
                     AQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALN
                     GKSSGGNDSTILTIPEGYRPPISFEQLVGS"
BASE COUNT         8961 a         3726 c         5379 g         6904 t
ORIGIN      
        1 gtacagcaac gaaacttcat ctatgcgctg ataggcgata ttttcatcga tacaggcatg
       61 ccaacggact tctggaagga attcttctac ttccgtttcg aaggtgtcac agggcgcaaa
      121 ataagcctca aagatgaatc gagcacaacc gtaagtgatg ccaatatctt agcgaatatc
      181 atcctagatt tcatctttga gcatgatatt ccgtttaaaa atggatatga aattttgccc
      241 caaaacgaac agtatttctt ttacaaatgc ctcacgaaca gggtgtgctg tagttgcgga
      301 aagaaaaatg ctgatataca tcacgtagac tctgtcggca tgggaaataa ccgaaaaaaa
      361 ataaacaatt ctggtaggcg ctttatggct ctgtgcagag aatgccatac gaaaattcat
      421 gtcgaaggtt ttactacatt tacaactaaa agaaagctta cggcagttgt tctcaaagac
      481 agtgatttaa aaagattggg gttgaatatc attgaatgaa ttatggatgg atatcgaagg
      541 atacgaggga gtatatcaag ttagtagctt aggaagagtt aaatctaatc gccctaacta
      601 tgtaattaat aagggatacg ttaacaaaga aggatttatt atgaaaccat ctgacaatgg
      661 gaaagggtat caaattatct tcttaagcgg caaaggtttt agagatagaa ggtatgtcca
      721 tcggctagta gcttttcact ttttaaaaga agcgtatttc gaaggtgctg aagtcaatca
      781 taagaacgga gataaatcta acaatactgt tgaaaaccta gaatggctca gttctgatga
      841 taataaaaag catgctagaa aaaatggatt aactaacttg aaaggaccat caaaactaac
      901 tgatttgcaa gctttagcta tcaagcgttt atatacaaac aaattgatga gctccggaga
      961 aataatgaaa ctttttaatg tcagcaggca taccgtgctg aatatagctt cagggaagac
     1021 atttagtttc ttggaagatt aagagaaaca caaaataggt gtgactaatt tcaaaaataa
     1081 atatcaaatc aaagggatca agttaaacca ggaaacaatt aaaaagttaa ggataggagg
     1141 ataaatttgg ctgagataag ttggatcaaa cttaaaacta ctatgtttga cgatgaaaaa
     1201 atacgattaa tccaagctgt tcctgagtcg gatgccatca tcgttatatg gattcgatta
     1261 ctagttttag caggaaagac taacgacgat ggtctgatat atatccagag gaacatgcct
     1321 tataccgaag aaatgcttgc tacattgttt ggcaaaaacg taaatacggt tcgcttagcg
     1381 ctaactacat tggcaaattt caacatgatt gatctaagca gtgatggact aattgccatc
     1441 agtaattggg aaaaacatca aaatatcgag ggtatggata aagtaaggct aaaaaatgct
     1501 gaaagaaacc gtaaatacag ggaaagaaag agacaggaac gtctcaaatt ggaaaatgac
     1561 gttagcgtga cgtcacgtga cggtacagat aaagatatag aagaagataa agatatagat
     1621 aaagaagaaa agaaaggtaa gtattctgac gaacacttac gccttgctaa aaagttgcaa
     1681 agtaatttaa ctgaagattt tccaaaagaa atgaacaaag tagatatcga aaaatgggca
     1741 gacacaatca ggttgatgga agaaagagat aaagcatcta tagaagcgat tgagtatgtg
     1801 atcaattggc tacctacaaa tgaattttgg tttggaaata ttagaagtgc taagaaattg
     1861 agagaaaaat ttgagaagct caaattcgaa atcaaagcag acaagaagaa tcataaaaag
     1921 caaagtcaaa aactacagta cagcaatcct agtgaatatg acgacttgcc aatttaaaaa
     1981 ggagatgcat cacatggaaa gcctagcaaa tgctatggag aaactaataa gaagagtatt
     2041 agtgcaaagc ggaaaatgtc cagaatgtag cgaacctttg tatagttggc gagctaaaaa
     2101 taaggatggt tcagaacgtt gtaaaccaac atgcatgagt tgtggttata aagcgttacg
     2161 tgtgaaagag gatatacaga ccgaacggat atataacgat agcttaaaag cacgagcgtt
     2221 gagttttttt caaaatggtt cggtattaac agataaaact ttgtttaaat gcaaaatgga
     2281 aaattatcac gtagtggatc aagaaacaaa aatcgcttta gaaaaagcaa aaagctatac
     2341 gaatgaggtt ctgctgaacc atcctgcaca tttcattcta tcagggaaat caggaagcgg
     2401 aaaaagccac ttatcaatgg ccacagcttg ggaaatactt gagcgctcaa attatgacaa
     2461 gaaaatactt tttataagct atcaagaatt attagagcaa ataaagtttt cttataacaa
     2521 tactgaactg agaaaagaaa ttgaaggatc gcttatagcc gatattaaaa caactgattt
     2581 ggtggttttt gacgatattg gagctgaatt aggtagtggg gtatcaaata gtaggcagtt
     2641 tacaaacaac acgttaaaca cgctcttaga agccagacag aacaaggcaa cgatcatcac
     2701 aacaaactta tctggtcctg aactaagaga agcctacggt gaaagaattg tttctaggat
     2761 atttaagaat tcagaaggtt atgcgctgaa attccaacaa acagcagaca agcgcataaa
     2821 accagtgaaa ggtagtatcg catgaataaa taccgtaata aaaaaactgt tcatcgaggt
     2881 atcaagtttg attctatcgc agaagcagag tattacgatc tagccttgtg gcaagctgaa
     2941 gcaaatggct ggaaagtaaa acttcaggaa agatttgagc tgatgccgaa atttgaacta
     3001 gacggaaaga agtatcgcaa gatcgagtat attcccgact tcacatttta taaaaacggc
     3061 aaacttgtca aagtcgtaga tgtcaaagga atgcagacaa aagactttaa gatcaaggca
     3121 aagttgttct gtcatcaata tcaagcgccg ttgatattag ctaaaaaata tcggaatacg
     3181 ttcaaggaag agcgttttta acgaggtggt ccatcatgac aacagaagaa gtgattcaaa
     3241 tgcgtattcg aaacattcag cgtgaaattg acgatctaga acgaacaaag gcagtgatgg
     3301 tcaatgaaac ggcgagaaag gcaatcgatt tgcacataga gaacttaaga agggaaattc
     3361 gtagattgga ggaatgagcg tggataagaa agcgacaatg aaacgaattg ctgaattaac
     3421 caagtcagaa tcttggcagg aagacaaaga aatagttgca gaagtccaaa agctcggtaa
     3481 atcaatgtgg actgaaaagc ctaaacggaa aacgccgaga aaaattgcaa tctggcatgg
     3541 tgatcgaatt ctagtaacag gtacagctga acagttatct gaaattactg gattaagcaa
     3601 aaacattatc tgggatagag ctaggagctt atggattgat tcaaaaggac gacagtttag
     3661 gtatgtggag gagagataat ggatctcatt acacaataca gtgacatcat cctcaagaaa
     3721 atcatgatga agattcagaa agacaaaaaa tcaaaagaac gagcggaatt agttaagttg
     3781 gaaatggctg aaacaggagc aggagtgcga agtagcaggc attggaaagc agcagcaaac
     3841 attgaatttt attacaacga aattcaaaaa gggttcgatc agatgcgtga gctggatcgg
     3901 caaacaaatt ggagcaagaa acttcatcaa gatcgtttca agtttgtaaa gaaatataaa
     3961 gagatattag acaaatacat ggaggacagc aaatgatact gaagtacagg gcatggaata
     4021 agaaaactca gtcatttatt gattatggtg atttggtttt agatttgaga agcggaaaaa
     4081 tttacgctgg ggacattggg ttagtggaaa gcaccattga tgtgactgac caaattgaac
     4141 tcatgcaatc cacaggaatg aaagataaga atggtgtgga gatatttgaa ggggatgtag
     4201 taattacatc aaaaggagcg attggatatg ttgcgtatct tcaacaagaa gccggctttg
     4261 tagttgtact aaagaaaagt gattatagat taggccatag gaatactggc gaaagttatg
     4321 atgtagcaac tgctcatgaa gtcatcggaa atatatacga gaatagcgag ttattggagg
     4381 tagagtgatt atcttagcta ctgactacat cgattcttta aaagatgaaa atgggaatgt
     4441 cccagcattg ctaaattgtg gaattaaaag caagaataga atcattttgt tacggatatc
     4501 ggctgattta gcaaaatatc tttatctgag caagacagca gtatattaca ctgcggctac
     4561 acgttatcag tacaagggaa aaacaatcag tcaggattat tatgatcgtt taatcagtcc
     4621 tgacatgcat ggaaaatcaa aatcagcgat caagttattt ggcgcaatag agatatatgc
     4681 cgatgatttt ccaaatttat ggttgaagga ggaagcggaa tgaacgagga aataaaaaaa
     4741 tctattcgtt acattgatag agcgttatcg caactcgaac ccttgcgaat tgaaagtcat
     4801 aaaggaatga gtgtctaccc aaatcagtat gacaaaatga acgctgattt acgtaatgca
     4861 aagctggcct taattaacat taaaaataac aaaggctgcc agtattgcaa acacagcgaa
     4921 ccgttgaatg ataccagcaa ctctaacttt gcaactgcaa ttgagtgtga ggaaaatgga
     4981 tactacattg caactgagta tgacgatggc gattatgcag ataatggcta ttctgcttat
     5041 atccaatatt gccctatgtg cggtcgaaaa ttggaggtag cggaatgaat gttcaagttt
     5101 atttaaaaag aaatttggcc aggtttcctc gaagctggga tacaaattcc cacccatccg
     5161 aggcagaaga agagagctat ctaatgtctg ctaacatgat agaaattaca agagatgatg
     5221 cggatgagtt tgataaaaaa agatcatggt actcggtttc gaatccaatg tatacggctg
     5281 taatagaaga ttatcatata tccgacagat ttattctggt tgatctagaa agacctaaga
     5341 aacgaattaa acagtataga cgttgcgggt tttcaacaaa gaaatggtga aaacaaaaca
     5401 gaggtgactc agaatgaaca acagacatcg cagaataaca aaactaagaa aacaggaact
     5461 gaatgtacta aagacaaagt ttgaaaaaga atacggagtt tctgcagaag aaacatataa
     5521 agtggcaagt cagtgtgttg ctgatgcaag tgatgctatt cgtaagtttg gaatttcgat
     5581 attaagtgat gatcgtaaat gggaggaaaa agaatgaaac taaaagacgg attttacgct
     5641 agtagccacg gtatcggcgg tttaatgctg gatatgcaga caaagaaccc taaaacacgt
     5701 aagaaaccaa aattcaaagt cggtgacatg gttcgctgcg aagcagaagg gttcatctat
     5761 ccatttcgtg gatatgtaga acatgtctat aatcactcag cgatcattcg cattgaaaac
     5821 acgatggaat gcgacaagtt attagcgaaa agcaaagaga atttagcagt ggtgagattg
     5881 gtggatattg aactaatcaa tgacaaataa aaaagccgga tcgctccgac taacataata
     5941 aaacagacaa gtttattata tcacataaag gagcggtttg acttgatgca attgttacga
     6001 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
     6061 ttggagcgta tggcaggtcg ctctttgata gatattaagt cgccgattat aacggatatg
     6121 ccgaaggcgc cgaagcacgg caataaggca gaggacgcga tcattcagat gatggatata
     6181 gaagcagaga gagatgcgat tctagcggct ttgatggctc ttagtctgat tagtcgtcag
     6241 atactctact acagcttctg cgacgtaaat aagcattcta attatgaaat aggtcaattg
     6301 atacgaggat acggagagaa gaatgtagag aagttgaaat ccatcgcatt gattgaattt
     6361 gctgaagcat ataaaaaagg cgtattaatt cagtatcgtt gattttgtag ggtttttgta
     6421 gggatagtgt agggtttttg agcggtttaa cgtgatatta tggtagtgtc gaaagattag
     6481 gaaacaggac ttcgacaaaa taaaatgtaa gggaggaaat ctccctcatc gtttaattaa
     6541 gcttcgatag acagcagcgg aaatattaag aataaggatg tgaattttaa ctccttctaa
     6601 attgttctta ttatctatca tccgttgctg tctattgtca ttatgtcact gtggcggaaa
     6661 gggtagacgc ttaaaaataa ggtcaatacg tcgagggata gccttaacgt tttatgattt
     6721 gaccatgcaa tgttcgattc attgccagtg acttaaggaa cctacggaaa caattcatct
     6781 tatcggatgc cgatgaattg gctgactagt cgggatgcta ctagcagtta gaaggcataa
     6841 aatactagcg cagacgtgcg ccactctcag gtgtaggtta ggagagaaac attagttggg
     6901 gttattagga atacgataac ctgcttgcga caaagctttg tactgtcgcg ttggtcatga
     6961 acagagacgg tattctgttt cagtattcgt tagcaaccga gggatgtggc agtggtgagg
     7021 tgcaggaagt atcagacttg tttgtgtgca agttactatt acatattaga tcactctttg
     7081 agtggtcttt ttattttgcg taaaggaggc acgacgatgt atagaccaca atacttagaa
     7141 cagaagcatg aagtaatcac tgtgcaaaac ggtaacggtg agatagtacg aaagtataga
     7201 agaccaataa agagcgatac atataaacgg aaggaaagca atgaagttat tccattgtat
     7261 ggcaaaagaa tagctaagta ttaaataaga ttgcgaaagg agatgggaca tgaccgagga
     7321 attctataga tggttattac agttgataag agaagatcgt ttggttaagt tctatcagtc
     7381 tcctaaatgg cgcaggctta gagagaaagc gatgaaacga gatcactatg aatgccaaga
     7441 gtgtagaaga ctaggtaagt atcatagagt agagaacgtt catcatataa aggaagtcaa
     7501 ggatagacct gacttagctt tagatttaga taatcttatt tgtttatgtg ttgaacatca
     7561 taatgaagtt catggcagat atcttacagc gttagataaa caagagaaga agatagaaag
     7621 cttcgctaac ttcgatgcaa gtgaaaggtg gtaagtgcat gatcatcaat gacaatggca
     7681 gagagtatga tacagaaaag attgaagagt attcatctta tactcaggga ttaattaaac
     7741 gtttgatata cgttcgctat gtaggtatta gggatctgtt atcagataac tgttgtagta
     7801 aatacaaagt gaatcaagta agagaagcgt tgaataaaga taataacgtc gaaagaataa
     7861 aaaatgtttt tggatatagt attgaagaga ttaattatta cattgacttc gctgaagctt
     7921 tcattccgat ggtgagataa ccccccctta aaataaatcg caaatttttt gggggtgatg
     7981 aaacggaggg ggctgtcagg aaaagagatt ttttcgaact ttatcatgaa aggagggcta
     8041 aaatgtttaa aaacgaattg tctcaaaatc gctacagaga aaaattacgc cgctctttaa
     8101 taagccaatt ggaaagtcag aaaacaaata ttgagccatt cttagataat gttgatcgtt
     8161 atatcagttt atgggaaacg gcgatatcac tggaagaaga tatatccgag aacggcatta
     8221 gactggagaa tggtaaaaag aatgaatcag tagcgttgct tgtttctgtc aacaaacaaa
     8281 tgggattgat gttggataaa cttgccatta ctcctgaatt ggtaggtgaa gcaaatgaat
     8341 caattcctga gttataagca tattgaaaat tggttcaaag ctatagaaga aggcactatc
     8401 aaggtatgca aagagcaatt attgctaaaa aattatctag aagaaagagt ctttactaga
     8461 gaagatattt acttcgataa gcagatggta gaggattcaa tcaatatacc agcacaatac
     8521 tttccattcg aattaattcc gtgggaaaaa tttctacaat gttttattta tggtgttcga
     8581 tggaaaaaag ataaaacact agtgttcaat agatatcttt cattaatggg acgtggtaat
     8641 ggtaaaactg gttttgcttc ttggaacaac ttctttctac taaccgctaa acacggtatt
     8701 aaaaattatg atattgatat ctatgccaat aatgaaagcc aagcaaagac tagttttgat
     8761 gatgtattta aagtaattaa agatcatcct gatttagata aaaaagtatt taaagctacg
     8821 aaggaagtta ttcaaaatat cgctacaaac agcaaacttc gttataacac ggcaaatgct
     8881 agaacaaaag atgggaagcg accaggtgca aaccgctttg atgaaattca cgaaaatgaa
     8941 gattattcaa tgataaatgt ggctacttct ggtggtggta aaaatcgaga ttatagagaa
     9001 ttttatgata caactaatgg tcatgttcgt ggtggtccgc ttgatgacat tatagaagaa
     9061 tcaaaaatga ttctttctgg agaacttgga attgacaagg atggagcaga attttctagt
     9121 ttgtttccat ttatttgtcg cttggataac gataatgaag ttgatgatcc cgacatgtgg
     9181 gaaaaagctt gtccaactat taattacaat gcaaatttaa aacggaaaat gtttcaagaa
     9241 tactctcaaa tgcaacgtaa tgctggttta agacttacgt tcatgaccaa acgaatgaac
     9301 agacctatgg aagatacacg atttgctgtt gcttcatatg atgatgttct gcatacgaaa
     9361 gaaaaagaat ttcctgaaaa aatggatgaa gtgataggaa cagtcgattt tgctgataga
     9421 cgagattttg ccagcgttgg gttgctagga aaatacgata aagatgttta ttttacacaa
     9481 catactttta tccacgaatc agcccttcga ttacaaaaca tcaaacgaga ggttatagat
     9541 atttctatag atcaaggaaa atcacagatc gttcatggaa aaaatataga agctgattat
     9601 attgtaggtt ggtttcttga aatgagtaat aaatattata ttaaaaaaat cgctatggat
     9661 atgtaccgtg caaaaatatt gaagcccgct ttagaagaag caggttttac tgtggaaatt
     9721 gttcgaagcg gatctgttac acatggtatg ttaaaagatc tggttgatga cctttttatt
     9781 aatcaacgtt tattttttgg tgacgatgcg attatgcgtt ggtattgcat gaatgtatat
     9841 gaagagcata tttctaatgg aaatatacgc tatgaaaaaa tagaacctga aactagaaaa
     9901 acggatggct ttttttcatt ccttcatggt ttgaattttt tagatgatat ttatgattct
     9961 gctcctgtaa cagtcacaaa tagctcagta gaaaatacag gaactggatt tactcctcta
    10021 gtattctaac ttgaaaggag gtgagaaagt ggggattttt caaaaggcgg taggatactt
    10081 cacaaaaaaa gcaacggtta ctttagaaga atacttttgt aaattgcaag ttgattttgt
    10141 gtatcgaaaa tttgcgattg aaacttgtat tgatttgatt gcaaatgcga tgagtaaagc
    10201 ggaattcaag tcatatgaag atggaaaaaa taaaaagaat gatctttact ataggctgaa
    10261 tgtagctcct aataagaaaa ataatgcaac agaatttaga aaaaaactga tcaggagatt
    10321 aatattctac aatgaagtat tgatcgtttc tccgtctaat aattctagcg aaatatttat
    10381 tgcggatagt tgggatgtca cagaatatgc attgaaagat gatgtgtttt ctcaagtgca
    10441 aattaacaac atagtccttg atagagaatt tctagaaagt gatgttatct atataaaata
    10501 cgcagatcaa caaattaggc aactagtcga tgcgtattat caagcgtatg ggaaactcat
    10561 ttctagtgcc atgaatgttt acaagcgctc taacgctcgt agatacgtac tgaaagggaa
    10621 tttattccga ccgcaagaca atacaacaca agatcaaatc aataaaatga tgacatcaca
    10681 atttaaggct tttatggaag ctgataatgc aggtgcggta tttcaattac aaaatgagta
    10741 cacattagaa gatttcagcg gaaacttcca aagcaattca agagatataa aaaacttaat
    10801 agacgacatc tttgagatga cagcagcagc gtttcacgtt ccgaaaaacc tactaaaggg
    10861 agacatgagt gggttatcgg atcaagtgga cgctttttta atgttcgaaa tcataccgat
    10921 tgctgaactt attcaggatg cgtttaacgc tagtctctat gaagtagaag aatacttgtc
    10981 agggaatttt gtacgtgtgg atacaactat gatcaagatt actagcttca aagatttggt
    11041 tgacgctatt gatgtaggca ttagaaatgg agtatttaca atcaacgaag gaagagaacg
    11101 cgttggaaat gatcgctctg ataaggcgat ggcagatgaa atatttataa ctaaaaacaa
    11161 tcaacaagta tcgaaaggag gtgaggcgaa tgacgacaat gaaaacattt ctagcagtaa
    11221 agaatgaagg cgcagtaccg caaattttta ttcagggatt tattggttct agttggttct
    11281 ttgaagggaa tactgacaag ggaatcaaaa atattttgga tagtctaggt gatcaagaag
    11341 aaattgaagt agtaattaat tcaaacggtg gagacgtatt tcaagggatt gctattggga
    11401 acttacttaa gtcaaataaa gcaaaagtta acgttgtgat taacggctta gccgctagtg
    11461 ctgcttcaat tatcgcaatg gctggcgata ctataaaaat ctacaacaat gcacaattga
    11521 tgattcaccg cgcttccaca tacggagaag gaaatgtcga tgacttccgt acgattgctg
    11581 accaactgga atcaattgat aaatcggtaa aggcttcata taaaacacga ttcaatggca
    11641 cagatgaagc attgcaagaa cttcttgaaa aagaatcgtt tatggatgca gaaacagctt
    11701 tgagttatgg attggtcgat gaaattatcg atgcagaaaa tagctcaggt actgaagcta
    11761 aaaaagaaca aagcgttgaa gaaattttga atgacgttaa agaaaaaaga gcagaaaaaa
    11821 ttgctgcatt tacagcagca ttaaataaaa catttggaca aggagatgta aaataatgac
    11881 agttaaaaat ttaaaaggtg taacagctgc aagcgaccaa ttgatgaaag cttttaaaga
    11941 tggtaacgaa gaatctttta gcgcagctat ggtaagctta tctaaggaaa ttcaggataa
    12001 aattttagaa gaagcaacag caaaaaatca agatcaatta gtattaatga accgtggtca
    12061 gcgtgtatta actacacaag aaacaaaatt ctataacgaa gtagtgaaaa acgaaggttt
    12121 tgcaggggtc gaagaattag tgccagctac tgtatttgaa cgtgtatttg aagatttaga
    12181 acaatctcat ccactattgc aaaaaattac ttttgttaac acaactggtg taacagaatg
    12241 gattgtgtca cgtggagtca atccagcatg gtggggtaaa ctttgcgaag ctgttaaaaa
    12301 agttttagat aatggctttg atgtaattaa catgaagcag ttcaagctat caggttatat
    12361 tcctgtatgt aaggcaatgc ttgatttagg tccagtatgg ttagatcgtt atgtccgtac
    12421 tgttttagta gaatcattga gaattgcatt agaacaagca attgttgatg gtactggtaa
    12481 agatatgcca gtcggaatga tgcgtgacat gagcaaacaa actagcggag aatatgctga
    12541 aaaaaaagca gaacctatta cagctttaga tgctgtaact atgggcggtt tgatggcgcg
    12601 actatcaaaa ttcaatatcg aaggtgtgaa tgatccgatt tatcgtaatg tgaatccttc
    12661 tgatgtggtc ctaattgtga atccaactga ttactggtct aaagtattcc cagctaagac
    12721 tgtactaact gctaatggag aatacgtaca agtattgcca gtaccagttt cagatttgca
    12781 gtcaacggct gtgccagaag gaaaagcagt tattggggta gcctcagatt acttcatggg
    12841 tgtaggatct acactaaaaa ttgaagcttc agatgaatac cattttgttg aagacgaacg
    12901 catttatcta gctaaacaat atgcaaacgg tcaacctaaa cgtaacgata gtttcattgt
    12961 attagatatt agcgctttgg gaactactac tacaactaca aaaccaacaa ccacaacaac
    13021 tacaacacaa gcgtaggtga tcagaatgaa gtatattctt tgtcagccgg caatcaatcg
    13081 gtttaaatgg gagcttgaag tttgtttaac taatctgaag aaactaggaa tcaaagatat
    13141 cgtattgctt ttcagcagac acgatgatca gattcctatt ttttttgaga aggaatatgg
    13201 tgttgaagtt catgtgtacg atgatctgcg ggacgacaaa gagtatattc cttcgattaa
    13261 accatattta tggtggaaat atttagaaga agatcattcg cgtgaggacg accgatattt
    13321 ctatatcgat tcgaatgtca ttttcaataa aagaattaat ttgcgcaaat tgccttctaa
    13381 agatgatgtt tggtattgta gcgactgctg tagttatcta agtcttgatt atattagaag
    13441 ctgtgaaaac ggagaaaata ttctaaaaga tatggcaaac attgtaaatg ttacagtaga
    13501 atctttggaa actataaaca ctaattcagg aggcgcacag tgggttatta accgtcctaa
    13561 agcgaattat tggaaaaagg tttatctgga ttctaatcgg ctatatcgct accttagagg
    13621 gcaaaaaaca aatatacaaa tctggacagc cgagatgtgg gcacagcttt ggaacatgat
    13681 gtatttcaat attggtccta aagttcacga ggaattagac ttttgttttg ctactgatcc
    13741 aatagaaaaa gttaaagaag taaaaatctt acacaatgct ggagtaacaa caaacgatga
    13801 agatttattt ttcaaaggga gatacgtgac ttctacgcct tttgatgaag atttatcatt
    13861 tgtaaacaag aaaaaatgct cttacgcata tgttaaagca attaaggcgg tggttagatg
    13921 acgcctgaac aagtgactga agaattgcta atagctgtga aggataatat ttacgttacc
    13981 tggaacgaag aagatgagtc aattaaaaag atgatagcta aaaatgctgt ttatcttcaa
    14041 agtaaagtga gtacaacact ttctttttct cctgaaagct tagaatacgg attgctaatc
    14101 gaaagatgta gatacgactg gaatcgtgct ttagatgagt ttgaacaaaa tttcgctagt
    14161 gagttattag gtttcattca acattatgcg ctacaagaat atattgcagg tgatgtgaat
    14221 ggcgaataat cgtagactcg aagaaacatt caacgatggt tggttaaaga ttttgacgca
    14281 aactaccaaa agaaatgaac taggaaaaaa gattggtgta gaagatacag aaatcacttc
    14341 tttaaaattt agaaatcttt ccatgagaga tagtgatata acagctatgg atgcgatggg
    14401 atcgaaatta actaagaaag taaagactcc atttcatcca atcgccaaga aatttaataa
    14461 agatcaatat tttatcgtaa tcgatagtat gcgttacaac gttatctatg ccgattacga
    14521 taatttttat atctattttt atcttgaaag tgtgggtgaa tatggtgatt gataattcta
    14581 aagaaaaaga acgtttaaat aagcaaattt ctgctatcaa aacttcctta gaagagcatt
    14641 ttggcctcaa actctttcaa gactccgtag gcgaggatga gctacctgat gattttaatt
    14701 acttcattct cgaaacagga gaaatagaaa tgatcactga gccaaaatat agcgtgggtc
    14761 aaaatctata tctaactttc tattcagaaa atagagaaga tttaacagga gactcactag
    14821 atattatttc attgattcaa aatcgttcga ttcgttttca gagaatggat cccaatcatt
    14881 taaaactaga gaaccaagat cgctatatcg atcaattggt atttacgttt agacgattat
    14941 tgaagagtga ttgtcatggc taaaaatagt tgggagctaa aaataaatgg acatgatgaa
    15001 cttcttgtgc ggatggaacg ctattcaagc gagagcgaac gactgattaa cgaagcattg
    15061 aaatcaaaag gttcggctat tgcagtggat aggattacag aaaaaattcc tgtttctgaa
    15121 gcagatttaa gaagagggca ccaacacgca aaaaatagtc gtccacttaa gactcaatac
    15181 attaatttgg gtttcatcat tagacctaca agaaaatttg agtatttaaa atatcctgat
    15241 ttggggatag gtacttctaa aagaaatcag ccggacgaat ttatgagaag aggattaggt
    15301 cttgcacttg atccaattac agaacttctg attcgtcaat tcgataaatt aaataaatag
    15361 ggggaacaac aatggctaaa acaaaaactg tagtaacaac gttcgataac ataagtatca
    15421 aacgaattgc ttttaatttt aagaacgcag gaaatgcaat cgtaacgaat tgtaatggac
    15481 aattagatgg cgaaacagaa atgcaaacgg tggttaaaaa atgtggagcg acagaagtaa
    15541 aatcaaaatc taaaccaatc aatatgacgg taacaattac tgcacatgta ccgatggaag
    15601 tttatcgacg tttcaatggg ttgaaacaag atggacgtat taaaccaggc atttactctt
    15661 acggtcctga ttccgtaggc gaagatttct cacttgctgc agagatcgtg gatgacttcg
    15721 aagaaaatag aaagttagtt ggtatgttag catgcacttc gaatacagga ttaacattct
    15781 ctattgaaaa tggtgcggat gaagtagctg cgttagaact agaaacaaaa gttatgcaag
    15841 atgaatttgg taaattttat catgaagcaa ttgttgcaga acttgaagaa gacttaacag
    15901 atcaatggat gacgaatcta tctgctgatg tgattaaaaa aacttcagtt gtgacaacta
    15961 cggccactca atcacagtaa aaaaaacgga ggtagcgaaa tgaacgaaga ttactcaaaa
    16021 attgaactaa acgatggaac aattttgaat ttagaaccta aactgaatat caagaaatta
    16081 ttgatgatca atagagattt taacacagac gagtttgcaa aaatgtcgat gggaaaaggc
    16141 tctatggata ttacagttat tcaaggtgca aaagccgtat atgtagctta tcgtcaagcg
    16201 aacatggtcg attacatttc attcgatgaa tttatagata aatgggattt tgatatggag
    16261 gttgcagtag ctgtatacag tactatgatg ttcaaacaag cacgtgatgc ctatcaaaaa
    16321 gaattcgaaa aagcaaataa ggaaaaaaag cttcaaaagt aaaaatgcca aagctcttag
    16381 ttgaaacgtg ggtcgatgtc tattcgatgt tgaccgacgt tttttctatg ccttcagatt
    16441 tggttttaag cgatatctgt ttagatgaca ttttgcaaat ggcttacaac aagagcgctt
    16501 atgaaggatg gaagaactat gcaataaatc aatcccagaa aaactaaagg aaaggaggta
    16561 aaaaatggct aaaaagagaa cggaagcaga agtaactttc atagctaacg atgacggatt
    16621 gaaatctacg ttaaaagaaa tcagtgctga attaactaaa aatagagcag aattaaaact
    16681 agaacaagct caattacaac agactggttc tgaatcagac aagttaggaa gtaaattatc
    16741 ttctttagaa aagcagtatg aattacaaag tcaaaaagtt gaagtaacta gtcaacgttt
    16801 agccaatgcc aaaaaatatt atggagaaaa ttccaccgaa gttcagaaac ttgaaagaga
    16861 actgattaat caacaaacag ctcagcaaag gttatctaat gatatagata aaacgagtaa
    16921 ggcattggct caatctaaag gtgaatttaa aacgtatgag tcaactatga aagacttaga
    16981 taatgagcaa caacatctaa agtcaagcgc atcattagta gaatccgaat ataaaaaatg
    17041 gcaagcaact gctggccagt ctgctacaga atctgaaaaa ttagcaaaag cacaagaata
    17101 tgtcggcaaa cagagtgata ttgctgaaca aaaaatcgaa gtgctaaaaa aacaattaga
    17161 tgccacacaa aaagaatttg gagaaacatc taccgaagct ttagaaatga aaactaagct
    17221 taatgatgcg gaaagagaat tcgaagaatt aagtaatgct gctaaaaacg ttgatacctc
    17281 cacagtagat gatatcggca aaaaacttga tatgggtaat ttaatggaag ctactgatca
    17341 cttgtcagtg atcggagata agcttattga tgtaggtagt aagtctattg aagcagctgg
    17401 aaaagcacaa gctatgcagg cccaatttaa acaagtcttt ggctctttag aaggggaagc
    17461 acaggacgcc gttgagggaa tggctgaaga atttggaatg ttaccaaata cgatcaagcc
    17521 tgtttttata caatatacgt caatgtttaa aggacttgga tatgatacca aagaagctat
    17581 ggagttagct ggtgatagta ctcagttagc agcagatgca gcagcttttt acgataagtc
    17641 tatggatgat gctagtgaat ctcttaattc atttataaaa gggaactacg aaggtgggga
    17701 gcaaataggt ttatttgcta atgatactca aatggcagct tatgctgtta agcataattt
    17761 gataccagcg actgaaggag caaaaaaagc cagtgaagag tcattgttag ctgttgaaaa
    17821 agcacaatct aagtatgctg atgctattaa gaaacatggt gaaggatctt tagaagcaag
    17881 agaggctgct ttaaaactta aagatgcgca agataaaata aatgaagaat taggcccaca
    17941 aacgcaaaaa tggtctgatt tagatgaggc taccaagcaa gcagttcgag ttcaatatgc
    18001 tgaagatatg caaaaattag ccggtgctac aggtcaggct agtagggagt ctgatggttt
    18061 agaaaaccaa atgacaagag caaagcaagc gttagaagat ttttatgcct cattgggtga
    18121 agatatactg cctgtgttta tcaaagggtt gcaagcagga gcgaaagctt tgcaaggatt
    18181 agccgaatgg tggagtaaac ttgatgggcc aatgaaaaat ttcattttag ctcttggagg
    18241 aattctagca ttattaagca cattagctcc tgttataacc gcagttgtta cgatagttgg
    18301 cacatttggt tctacagttt tgctgccaat aataggaatt atagcgggcg ttgcagctgt
    18361 aatagcgatt gtaattacag cgtttcaaaa ctggggcgca atcactgact ggtttagtga
    18421 tttatggaaa aaatttaccg attggttagg tgatacctgg gaaagtataa aagaaagtgc
    18481 ctcatcagtt tgggatggag tcaaagaaac ctggtctgga tttgtagatt gggttcaaga
    18541 aatttggcaa ggagtttctg attggtttgg agagttatgg agcggattag ttgaaggagc
    18601 ttccaacatc tggcaaggag tccaagagac ttggcaagca ttcgttgatt gggtttcaaa
    18661 tatttggaac ggagtcaaag aagtatggtc gattatttgg gcagacattg taggaattgt
    18721 tcaaatacca tggacattaa taacgtcatt gattcaagct ggtattaata ttatcgtggg
    18781 tatctttgat gtagctggac agttattagg cgcagcttgg caagctgttt ggacacctat
    18841 ttctgatttc cttaaaaata cttgggatac tatgacacaa tggataagca tcgcttggaa
    18901 tggaattgta actacattcc atactatatt tgatccagta gtggcatggt ggaatggtat
    18961 atggacatct attagtacta cggcttcaaa tatttggaat tcaattagtg caacagcttc
    19021 tagtatttgg aacagtatca agaatacaat cactagcttg gtacaagcag ctgctacagt
    19081 aattcaaaat atttggtcaa ctgtatctag ttggttaggt ggaatttgga attcaatcag
    19141 ctctacagca tcaaatatct ggaatagtgt gactagtagt ataagcaatg ctataaacgc
    19201 agctaaaagt gccattcaaa gtgtttggaa tagtatatct tcgtggatca gcggaatttg
    19261 gaacggtatc aaaaatactg ctttgaatct ttggaatgga attacaagca ctattagctc
    19321 taaagtaaac gatggaaaaa atgcaatttc aagcggttgg tccaatctaa caggtattgt
    19381 ttccgacata ttcaataatg ttaaaagtac aattgctaac atatgggaag gcatcaaaaa
    19441 gactgttagc gctccaattg attggattag agacaaaatc agtggcattt ttgataattt
    19501 gaatatttct ataccacata ttccgttacc ggaatttatc atggaaggca gctttaaccc
    19561 gctaaaaggt caaatccccc atttgcgtgt aaaatggcat gctaaaggag gtatctttac
    19621 taaaccaact ttactaggtg gaatgaacgg tgtcggtgaa gcaggacctg aagcagtttt
    19681 acctttgaaa agatctgttt tgcaagaaat tggtgatcgt atcttgagta gcacatcagt
    19741 ttcatctagg gcacaaacga ttcaacctgt gaataactac gaattcaatt tcacaattga
    19801 tggtaacgca gatgaggtta ctatgaagca aacaactcaa caaatcattg atagcattac
    19861 aaaaattcaa aatgataatg cttcggcatg gcgttaaaca ggagagtatt tctcctgttt
    19921 ttttagtatt aaaaaggatg tgaaaaaatg actgattgta tacattctat aatcgatgga
    19981 tttcctgatt atttgcataa attggcttta gctgaaagac caaccatacc ttctccaaaa
    20041 agacagagag ttgaaacttc tgttttaggt aggttaggtg gcttagtaca agattactcg
    20101 tttgaagata tgtcgtttac attgcactat aactatttag aggatgtgga agaccatcaa
    20161 gcgttcaagc aatcgtttta tatcatgcgt cattggttaa actatgcaaa gaaattagaa
    20221 ttctctgacg atcccaacgt ctattacgtt atccagacta tcgatattgg ggatgcagaa
    20281 aacgatattg ttgaatgggg agagttcgat gtaaatatta ctgcgaaacc attcgcaaga
    20341 gttcaagaag atgtacctat aaccgtagat aaaccacagt catttaactt gctgaataat
    20401 agtttagaag aaagttttcc aaagattatc atcactcctt cagctacttc atgccagttc
    20461 atcttaaatg attatgtgtt tagtttcgaa ggcttagtag caggaactga tgtagtcatt
    20521 gatagtgatt tgatgctttg ctacgaagag caatcggacg gagatatttt agatcggtcc
    20581 aacaaaatga agaccatgca atatccaacg ttgcaagtgg atattaatca ttttaattgt
    20641 actggtttga gcaaaataca aatttatcgt aacgggttaa ggtaggtgaa atagatgatc
    20701 gataatttaa taactattta cgataaaaat gacgcgaata atttagctga acatttatat
    20761 gatacgcaag gtttaggcgc tttgtcagac tggttaacag ctactgttag caataaacta
    20821 aacggagccg agatatttca gggtacttat tcaataaacg gaactaatgc agatttgatt
    20881 gtagaaggac gtattattca gtgttatgta gatgaaaatc gagcaaaaca gcgtctacgg
    20941 atttattatg caaaaacttc tgtaataggc aatacgatag aagtaaaagc tgaacctatt
    21001 ttcaatgata taagaaaatc ggtgttgaat aaatatgaca gcggaacaga aaagatcact
    21061 gctactcagg catggcaaaa cgcaaaagtt ttagcgaaac cagctatccc ttcgcagttt
    21121 tctttctcgt cattagtaga tacgcttgct aatgtgaaga tagaaaaggc gaatttttta
    21181 gaattctttg gtggaaaaga gggatctatt ctagatcgat ttcatggtga gtttctaaaa
    21241 gataataaca cattacgtca tgaaaaaagt ctaggtacgg atcataaaat caaagcgatt
    21301 tatactaaaa acttaactgg tcttgactta gagattgatg ctcaaagtgt tttagttgga
    21361 gtttatccat tcattagcag ctcttcagaa ggagaagacg agatcactct accagaagaa
    21421 gttattttca cggattacgt ggatgattat cctgctggat atgtttcttt tgttgatttt
    21481 aaagacaaag cgactgatgt agccacatta agggaagctg ctaaagactg gttgaaaaca
    21541 aacatagata aacaaaaacc acaagtgagt ggttcgattg aattagtacc attgaggcat
    21601 caaagaggct atgaaaaatt tgttgatcta gaaaaagttt cgatgggtga cggagtagat
    21661 gtgtatcatc cacagttaaa agtgaatatg tcagcgagaa ttgtggaata tacgtttaat
    21721 gttctaacca attcatacga taaattagtt gtaggaaacg tcaaaacaaa cttcttagaa
    21781 aacacagaga ataatgtcag caatttgatt aatgatgcca ttgatcaatt gaaaaatggt
    21841 ggcgaaatca gtgatttaat caatgatatt gtagatcatc aaactgatat gattactggt
    21901 caaaatggtg gttacgtttt attagatcct aaagaagccc ctagtcgtat tttgattatg
    21961 gacacaccag ataagaatac tgcacggaac gttttgcaaa tcaacaacgc tggtattggt
    22021 ttctctaaaa ctggcattaa tggaacgtat gacaccgcat ggacgttaga tggcggattc
    22081 aatgcctcgt ttattacagc tggtgagata gtagggatta ctattagagg tactacatta
    22141 attagtgatg gcactgatta tagaacaagt atagctaatg gcaaaatgac ttggtactca
    22201 aaaaaagtta acaaagatat tatggagcta gaagcacgtg attatgtaag tgctgatgcc
    22261 ggcattgtat catacaccat gaaaactggt ggtggtttca tgattagaaa tccacagggt
    22321 aacttggttt ttagtacgtg ggataatggt aataacagac cgtttttatc ttttggtgcg
    22381 ccaaatttca gatacagtaa tgctagttat ataaatgatg gtgacggtaa ttctttaggt
    22441 attgatggta gtgcaggtaa ttcatgggaa tttaaagtag ctggtaggac tatgaaattt
    22501 actagtgacg gtatgctaac gttgccaggt tgtttttttg gttcatggga agatgggaaa
    22561 cttgctagat ttgaacaatc aacggtacaa gtatataaag attttactgt tagaggtact
    22621 aaaaactcaa ccgtaccaac agaacattat ggacaacgac tattgaacgc ttatgaaact
    22681 ccagaatatt atttcgctga ttatggggaa gccgttacag gtgatgatgg taaagttcgt
    22741 gttgatattg accccatgtt tgctgagaca gtaaatctaa gtcggtatat gacacatgtg
    22801 acacctacag aactagtttt gtgtgctgtt actcatgaag atgttgacca tttcatcatt
    22861 gaaactagta agccaaacgt attggttaga tggaatttag tggcacatcg actagggtat
    22921 gaagatgtta gattaaaaga ggatacagca tatgatagca cagtgcttga ccaaaaacgt
    22981 ttttaaaacg aagacaagga ggtatataaa tggctagcag tttatataat ttggctttag
    23041 atttcagcaa agaattaaac tacaccaaag ctattatggc tcgtcaaggt gataaaggga
    23101 tcacggtgac tgttaaaccg tttttaaatg gcttgcagat ggatacgagc ggcggaacat
    23161 ttactttaaa aggaactacg ccatctaacc gttacgtaga tagtgtcgct actagtgtaa
    23221 ctagtgaaga agtcacattt tctcttgatg gcacatttat gagtgaagca gggtattata
    23281 aacactgcta tgtagaatat agaaaagacg atcaaatttt aacgacgcaa gatatcattt
    23341 ttttctcact aggagtgtct gacatttcgc aaggccaagc cgatgaatat gtttcgcaat
    23401 tagaagaatt gattcgaaag tataacgaaa cttttgatgc ttttctggct gaaattaaag
    23461 gtagaatgga tagcttaaat aaacagatta ctgatttaac tggtcaagct aaaacactac
    23521 aagacaagtt agatgctctg aaagaagaaa tttctaaatt aggtaactta catgtgatgt
    23581 actccaacag catcgacttc gggggctatg attatagtgg gaatccgaat ttaatgtcca
    23641 aactaaaatc gagcgatttt aacgttggtt accacgggtc actaactaat gttaacggaa
    23701 tgttacattt tatgtctgat ggtacagggg acattgatat gtttacgcgt attaatacac
    23761 ctcagcttgc tagtgggaaa acctatactc tgagtgcgaa agttcgattt gatgaaggaa
    23821 ctacaggagc tattgataaa ttacgtttgg tgtatcgtac atcaccagga gaaaagatat
    23881 tattggaagc aaataataca actattacaa tagatgatgt agggaaagaa ataacaatca
    23941 aaggcacagc taacgttaat tatcaaatca caaatttaga tcgtttttat attagtgtta
    24001 gtttttctaa cagggataaa ataaatggtg gatttaaatt gtacgacatc aaaatcgaag
    24061 aaggctcaac agccactccg tatcagccaa acttactaga tgacccttac tgggtaggta
    24121 aaacatcgtt gggtaagaat attgcaaata aaggtacgac gttcccaatt aaatctagcg
    24181 cgtacgaaat atataaaggt aacacggaag aagagcttat aataggtcaa acgtatacta
    24241 tcacgcttaa aggaacaaaa cccgcaagtc aaacctttgt agcatataat tattggaacg
    24301 ttaattttgg agatctaaaa ccagttgagg gattgacaga cgtatggtct ctaacattca
    24361 caccaacgaa acttgaaccg ggtttgccta aagaccttcg catttttcag tcgcctaaag
    24421 aaacggccgg cgcatgccga attgactggc tcaagatcga aaaaggcgac acacgaaccc
    24481 cgaatattga gcaatataaa taccgaggaa tcggcatgcg agactcaaac aatccaaaag
    24541 attatgtatg ggatctagaa cctagctatg ttgagaataa cctagctaca gatatcaagg
    24601 ttgctgaaat cacaggtaaa gcaaacaatt ataccgatgg gaaagtatcg gagattaatt
    24661 cgcagttgac tgcttcaatt aatgaagtag acaccacagc taaggatgct caaacaaaag
    24721 cgaatgctaa tgcgactgct atagatgaat tagacaataa gatcgatgaa cgcattaatg
    24781 atacagctac taccacatta acagttacaa acgggaatac cggatcagca aagctttatc
    24841 gtgaaggaaa aacagtttct atatattttg tggctttaaa cggaaaaagc agtggtggaa
    24901 atgattcaac gatactaaca attccagaag gctatcggcc accaattagt tttgagcaac
    24961 tggttggctc
//
LOCUS       DAZKOR010000038        24386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000038 DAZKOR010000000
VERSION     DAZKOR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.038"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..614
                     /locus_tag="ACG8ZM_001438"
     CDS             <1..614
                     /locus_tag="ACG8ZM_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0492403.1"
                     /translation="VFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVGK
                     YMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGTG
                     VFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPFI
                     LALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            611..1384
                     /locus_tag="ACG8ZM_001439"
     CDS             611..1384
                     /locus_tag="ACG8ZM_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0492404.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1381..1779
                     /locus_tag="ACG8ZM_001440"
     CDS             1381..1779
                     /locus_tag="ACG8ZM_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0492405.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1964..2635
                     /locus_tag="ACG8ZM_001441"
     CDS             1964..2635
                     /locus_tag="ACG8ZM_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492406.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2694..3134
                     /locus_tag="ACG8ZM_001442"
     CDS             2694..3134
                     /locus_tag="ACG8ZM_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492407.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3146..3814
                     /locus_tag="ACG8ZM_001443"
     CDS             3146..3814
                     /locus_tag="ACG8ZM_001443"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0492408.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3878..4501
                     /locus_tag="ACG8ZM_001444"
     CDS             3878..4501
                     /locus_tag="ACG8ZM_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0492409.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4557..4943
                     /locus_tag="ACG8ZM_001445"
     CDS             4557..4943
                     /locus_tag="ACG8ZM_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0492410.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5156..6547
                     /locus_tag="ACG8ZM_001446"
     CDS             5156..6547
                     /locus_tag="ACG8ZM_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0492411.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            7158..7526
                     /locus_tag="ACG8ZM_001447"
     CDS             7158..7526
                     /locus_tag="ACG8ZM_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0492412.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            7706..8647
                     /locus_tag="ACG8ZM_001448"
     CDS             7706..8647
                     /locus_tag="ACG8ZM_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0492413.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            8716..9447
                     /locus_tag="ACG8ZM_001449"
     CDS             8716..9447
                     /locus_tag="ACG8ZM_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0492414.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            9591..11771
                     /locus_tag="ACG8ZM_001450"
     CDS             9591..11771
                     /locus_tag="ACG8ZM_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0492415.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            11791..12273
                     /locus_tag="ACG8ZM_001451"
     CDS             11791..12273
                     /locus_tag="ACG8ZM_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0492416.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            12289..13050
                     /locus_tag="ACG8ZM_001452"
     CDS             12289..13050
                     /locus_tag="ACG8ZM_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0492417.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            13181..14515
                     /gene="murC"
                     /locus_tag="ACG8ZM_001453"
     CDS             13181..14515
                     /gene="murC"
                     /locus_tag="ACG8ZM_001453"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0492418.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            14822..15307
                     /locus_tag="ACG8ZM_001454"
     CDS             14822..15307
                     /locus_tag="ACG8ZM_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0492419.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            15300..15983
                     /locus_tag="ACG8ZM_001455"
     CDS             15300..15983
                     /locus_tag="ACG8ZM_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0492420.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            16109..18754
                     /gene="polA"
                     /locus_tag="ACG8ZM_001456"
     CDS             16109..18754
                     /gene="polA"
                     /locus_tag="ACG8ZM_001456"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0492421.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHANTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            18799..19635
                     /gene="mutM"
                     /locus_tag="ACG8ZM_001457"
     CDS             18799..19635
                     /gene="mutM"
                     /locus_tag="ACG8ZM_001457"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0492422.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            19599..20228
                     /gene="coaE"
                     /locus_tag="ACG8ZM_001458"
     CDS             19599..20228
                     /gene="coaE"
                     /locus_tag="ACG8ZM_001458"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0492423.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            20280..20612
                     /locus_tag="ACG8ZM_001459"
     CDS             20280..20612
                     /locus_tag="ACG8ZM_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0492424.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            20924..21415
                     /gene="nrdR"
                     /locus_tag="ACG8ZM_001460"
     CDS             20924..21415
                     /gene="nrdR"
                     /locus_tag="ACG8ZM_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0492425.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            21438..22811
                     /locus_tag="ACG8ZM_001461"
     CDS             21438..22811
                     /locus_tag="ACG8ZM_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0492426.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            22811..23737
                     /gene="dnaI"
                     /locus_tag="ACG8ZM_001462"
     CDS             22811..23737
                     /gene="dnaI"
                     /locus_tag="ACG8ZM_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0492427.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            23866..24306
                     /locus_tag="ACG8ZM_001463"
     CDS             23866..24306
                     /locus_tag="ACG8ZM_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0492428.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         8161 a         4172 c         5372 g         6681 t
ORIGIN      
        1 ttgtttttgc accgttagaa ggagaagacc tcgcttcatt tctggttggt gcggcaatca
       61 tgatgatcgg aatgacgctc tttttgtttg gagcagatta ttctatgatg gaagtcggag
      121 atctagtagg gaaatatatg atcaaaaaga aaagtttgac cgttttggtc agtttgggat
      181 ttgctatcgg gatcgtgatc acgattgctg aaccttccgt gcaagttttg ggacaacaag
      241 tatatgatat atctgacgga gagattggca gagtgttgct gattgggatc gtcagcgtcg
      301 gcactggagt atttctagcc tttgcgctgc ttcgggtcgt attcaagcta tcctattatc
      361 aattgatggc tataggctac ataggcgttt tgatcgcctc atttttcacc agttccgaat
      421 ttatgccgat tgcttttgat tcggggggag tgactactgg tccagtaact gttccattta
      481 tcttggccct tgccggaggg atgactagta tgatcaaaca aaagaaaaat gaaaatgaca
      541 gttttggaat ggtcggtatt gcttcgttag ggccgattct tgccgtgatg attttggggg
      601 tgatcttcca atgagtttct ggcagcaagt gatagatgga atcgaaatcg ttaccatgga
      661 agtcattttg gcagtggcac ctatcgtaat cctatttgtt ttaatgaata tcattagttt
      721 tcgtatgcgc aaaaagcgtt tctttgacat tatgaaaggt ttcttgataa cgactattgg
      781 gttgatcctt ttcctgcacg gagtcaatat ggcttatgta ccggtaggac agcatttagg
      841 cagtgctatt gcgggattgc cttacaatta tatactggtt ccccttggct ttttaatggg
      901 gttcttagta gggtttgctg agccggcgat tcatgtaatg atcaaacagg ttgaagaatt
      961 gagcgagggg agaatccgat cgaaaatcat gctgatggta atttcaattg ggatcggcgc
     1021 agcagtagga ttgtctatgt ggcgtttgct cgtcggattc tctctttatt atttcttgat
     1081 cccaggatac gggctagcat ttattttagg acgaaaagtg gacaagatct ttttagcaat
     1141 ggcttttgat aacggtggag tagcaacggg cccgatgtgt tcgacattta ttttatcaat
     1201 gagcgtggca atcgctgcac aaattgaagg aagagatccg atcattgacg ggtttggtgt
     1261 ggttgcaatg attgcattga caccaatatt atccacttta gtactaggat atatttataa
     1321 acgaaaagat gaaaaagaca gaatcgaaaa agaaagattg caaaaattgg gggaaagcaa
     1381 atgaaagatc ttgtagcatt gaatcttcac atgattgtga cgatcgttga cggaggaatg
     1441 ggcgaagatg ttatcgactt ttcaaaagaa gcaggtgcag caggcggaac gatcttacat
     1501 ggaagaggtt ctggtgtaca tgattcaggg aaatttttag gtttggacat ttcgccagaa
     1561 aaagatattg tgttgacatt agtacccgat agtttgacga ataaagtaat ggatgcgatt
     1621 ggtcaaggca tcaacatcga tattccaggt aatggtattt gttttagtat tgatgtagac
     1681 aaagtcattg gtatcactaa gattcatcaa tatcgagaaa ttatcggaat gcaagaactt
     1741 ttagatgaaa ataacgatag agaaaagtct gaatcataga caagcgtttg aagaggaaat
     1801 aaacacaaac aagaaggcgg atgagcgtta tttccgcttt tttgtttgta tttttctcgt
     1861 gaatacttat gaaatagtaa atttttatga aattctactc taagcatcag actttctttt
     1921 caaaatatgc tattttaaaa atgatgaaaa aagggggaga aaaatgaatt ttttagttgt
     1981 tttaaaagat actctgattg agcgatttac atttctatat gttcaattgc aggaaaataa
     2041 aacattgctg catttttcac cagagttcca tctagagggg ctaacattag gagaaatcta
     2101 tcaagcagaa gggctgtcgg ctgttttgca cttttttaag gtagaattgg aattaccagt
     2161 aaaagattat atcgagcttt ctcttgaatc ttgggatcga gcagtggttg aactgtttcc
     2221 agaaggttta atgatcgata caaacgatgg gaagatccat ctgactgcag cagaactgca
     2281 aaaacaaatg cgttatcaaa tagatgacga aaattctttt tctattttta gacgtcagca
     2341 aaagattcta aaaagctttt taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac
     2401 acagctgttg aaaaaatatc caggtctgct tcacacgtct attcaatttc ctcaattgat
     2461 tacgatgatc catcgtttta tacgtatgca acagctacca tctaagaagc tgtttcttcc
     2521 attgacggac acatttcgag tagtcaacag agaagataaa aagaaagtga ttgtgattga
     2581 ctttaccaga aatcggaatg tccttcacca agttgcaatg gaaaaggcca attaaggctt
     2641 tttctttttg gatttttttg tcataataaa tattagatga tggaggagat aatatgatgc
     2701 aaacatggtt agaaaggcta atacgatttc agcagaacta tcacaaactt atgcagggaa
     2761 gatatgcgaa atttgatcag ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt
     2821 tgctaagtcg ttggttgccc tatagaatcg gacaattgtt gttcatcgtc tttttcggat
     2881 ggatgatatt tcgtttccta tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt
     2941 atttgaaata tcttgataga gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag
     3001 tcaaaatgga tcgaaaaagt tatacttttt ttgagtgccc gaattgtcat caaaagcagc
     3061 gtgcacctaa aggaaaagga cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg
     3121 aaacgaatgt ttaagggaga aaagaatgaa attagtatta ttacgtcatg gcgaaagcga
     3181 agcaaatttt gaaaattact ggacaggatg gctagatgtc gccttaacag aaaaaggaaa
     3241 agaacaagca gcagttgccg gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt
     3301 ttatacctct gttttgaaac gtgcgattct taccggacaa gtcgcactag aagaaatggg
     3361 tcagttatgg cttcctataa taaaaacatg gcgtctaaat gaacggcatt atggggatct
     3421 tgtgggtaaa aacaaagata agatgaaaga aatatacgga aaagatcaag taaaaacatg
     3481 gcgcagagga tatacggaag ttccgccgtt aaccgcagaa aatagatttg accgcagata
     3541 tgatcacttg gatccgcatt tgatcccata cggagaaagt ctggaaatga cagtgaatcg
     3601 gatcattccc ttatggcaag atcatctagc accaaaacta aaagatcggc aagatttact
     3661 agttgtgggt catggtaata gcatccgagc actgacaaaa tatttagaag atgttcctga
     3721 agatcaaatg gatacgatcg atatcccaaa tgcacaacca atccagtata cattagcaga
     3781 tgatttgaca attctggaca aaaaaatact ttaaagaaca cctttatcta aaagtgagag
     3841 atagaaaaaa gaagcaaagg aatggaacaa aaggaacatg aaaaattata tatgggactt
     3901 tgatggtaca ttgtttgata cgtatcccgc aatggtagat ggtgcatggc aagcactaaa
     3961 agattttggt atcagtatgg acaaaaaaga aatctacttc aaaatgaaaa agtattctac
     4021 tagttatttg atcaatgaat caaacttgaa tgccagagag tttaatgaat tatttcaccg
     4081 atatgaaaaa gaaagtacgg aagtttcccg tccatttcca gaaacgaaac aagtactgga
     4141 aatgctaaaa gacaacggcg gtcgtcattt catcctgacc caccgtttga cagaatcaac
     4201 atggggcctg ttaaaagaac atcgattggc acatctgatt gaggaagtag tagggatcga
     4261 tcaggacttt cctcgtaaac ctgatccagc atctttaaat tatttgatcg atacttttca
     4321 tttagaaaga acagacacga tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa
     4381 aaatgcagga gtcgctactt gtctttacga catcgatcat tttctgggtg agatcccggc
     4441 agattatgtg gtaggaaatc tgaatgaaat ccttaccttg cagagcatca ttaaaaagta
     4501 gtatcccctt tcaaaggaga tatgttataa ttttcataaa ggagtgataa aattatatgg
     4561 aaatcagaag acaaaaagaa gttgtagaag catttcatta tgatatgaga ttgccgaacc
     4621 aagaagtaga aacagatttg aaagtaggct tctcgccttt aaaagaaacg atcgaaaact
     4681 atccgaaaga acatagcatc gtaggagcac gcttagagtt ccgtttagtg tttgatacat
     4741 atgttttatc tggaagtgtg agtcagatca atcatatcct caatcggaaa attgaaggtc
     4801 aaaatgatgt cacacaagaa gaagtggacg agctagtagc accgttattc gatatcgtac
     4861 gccgaatgtc ttatgaggtg acagagatcg ccttagatca gcctggggta aaattgaatt
     4921 ttcaatcaga aaaacaagaa tagacagtaa aaacatacct gcgatagatt aaagctatca
     4981 caggtatgtt tttttattac taattgtaag caaaaagaat gtcatttcga taaaaatcgt
     5041 ctaaaatata aaagtcactg aaaaaaattt tttcaggagg atggttgaag aatcattctc
     5101 gggaaccttt acttccttcg atggacttgt cattaataat atgaggagga gaaaaatgac
     5161 aacactcatc acagttttag tggttctagt ttttgcggca ttattatatg tgttctatcg
     5221 tatgcaaaag cgccatacaa aattttcaac acgcgtcttt acagcattag gagttggtat
     5281 cgtctttggc ggtctgcttc aattgatctt tggtgtagac gacaaagtcg ttactcaatc
     5341 aatggaatgg atcgggattg taggtcaagg atatgtatca ttcttgcaaa tgctagttat
     5401 gcctttagtg tttatttcga tcattggtgc attcacaaaa atgaaagttt ctgaaaagct
     5461 agggaaaatt agtgcgactg tattgacttc tttacttgga acaacagcta ttgcagcatt
     5521 gatcggaatt ggttcaacaa tgctgttcgg actccaagga gctaaattca cacaaggagc
     5581 tgcagaaaca gcacgaatct ctgaactggc aacacgtcag gaaacagttg aaaacttatc
     5641 gattccacag cagattttat cctttatccc taaaaacgtt tttgctgatt tagcaggtag
     5701 tcgtgcaaca agtacgattg gagtcgttat tttcgcggca tttgtcggaa tcgcttatct
     5761 aggcgtgaaa agaaaagcgc caaaagaagc agcattcttt tcagatttag tcgacagtct
     5821 gtataaaatc attatgcgga tcgtgacgct tgttttgcgt ttgacacctt atggagtatt
     5881 tgctttgatg acaaacgttt tggcaacaag tgattttgca gctatcgtca atttaggtaa
     5941 atttgtcgtt gcttcttacg tagcattgct tgctgttttg attgtccact cactgatttt
     6001 gattggtgca aaagtcaatc caatcaacta tttcaaaaaa gcctttccgg tattgagctt
     6061 tgcgtttact tcacgttcaa gtgctggagc tttgccattg aatatcgaga ctcagacaaa
     6121 agctttagga gtagatgatg caacagctaa tttctcagca agttttggtt tgtctattgg
     6181 acaaaacggc tgtgctggcg tttatccagc tatgttggca acgatcgtcg caccagcagc
     6241 tggagtgaat gtctttagtt tggaatttat tttgatgctt gtggcaattg taacaatcag
     6301 ttcttttggt gtagctggag tcggtggggg cgcaacattt gcttcactga tcgttttagg
     6361 ggcaatgaac ttgccagtag caatcgttgg gttagttatc tctgttgaac cattaatcga
     6421 tatggcacgt acagcagtaa acgtcagcgg tagcatggta gctggtatcg tgacaagtgc
     6481 acgcatcaaa gatttagatc gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa
     6541 tgcatagtca aaaagaggtt gtgatagaag tggtcagctt taagaaataa gaagaagttt
     6601 cctaaaaata gtttctcaaa tttttgtgaa atttcggctt atttccgaga agctacttct
     6661 gaaacaccgt gaataagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
     6721 aaacaaaaat agttcctcat attttacgtt tggttgagct tattaccgca gatgcaagct
     6781 tttgaacacc gtttaagaaa gaggtcgtga gcctgccgtt tcactctgag tcacaaagag
     6841 caatttcaaa aaatagcttc tcatgtttgt tgaatattgt gtcttgtgac cgaggagttg
     6901 tcaggagaac accgtttacc agaggttgtg acttgttttt agtcacgacc tcttttttta
     6961 tttattccat ataaatacac attatttatt ccgaaaagtg aaagcgcaaa taacaaaaac
     7021 aaccaactta tttattatta aagaaaagcg tatttaagta ggacgattct gttgagagct
     7081 ttcgtagatt ttcccatttt cctatggtaa actgacaaag ttgagaatgt tagaacagat
     7141 cgtttaggag gaaacagatg tttacgaatg aaataaaaat catgctttat gtggacgatg
     7201 tagaaaaaaa tgcaacgttt tggagagcag ttggctttgc ggaaaaagat cgtcaagaaa
     7261 tggatgggac gcttataatt gaagtaggag ttgaggacag tcaaacctca tttctgttat
     7321 atgatcgagc atttgttgag tcccattcac ctggcacagc tactagtgcc ccatctttga
     7381 tgttttacag taaagatatc tttggactat ataaaaaaat gcagacgcaa tcagtcacat
     7441 taggtgatat ggtacagctt ggagaagaat atgtctttaa ctttgctgat cccgatggca
     7501 actactttgc ggttacaggt aaataataga aataaagact ttgtttttct gtgcgaaaca
     7561 ggaaagcgaa gtctttttaa ttcaaataat aatattatgt aaacaaaaaa ctttccatgc
     7621 gtaattatac acaagcaaat aaatcgtcaa atgcgaacgc cttctttata ctgtttagga
     7681 acgaaaagaa ggaaggaaga aatacatgga ttatcaagta ctgctttatt acaattatac
     7741 aaaaatcgaa gaaccagagg aatttgccaa aaatcattta gccttttgca aatctttgga
     7801 tcttaaaggt cgtattctag tagctgaaga aggaataaat ggtactgtgt ctggaaccgt
     7861 tgaaaacacg actgcctata tgaaagcaat gaaagctgat cctcgatttg ctgatacgat
     7921 gttcaagatc gatccaagcg aaggacatgc ctttaagaag atgtttgtaa gataccgtcc
     7981 agagcttgta agtttaaagc tagaagagga cattgatcca aaccaagtga caggtgccta
     8041 tctttcaccg caagaattta aagaagctat tttagatgaa cagacagtgg tgattgatgc
     8101 acggaatgat tatgaatatg acttgggaca ttttcgaggg gctgtacgcc cagatatacg
     8161 cagttttcga gagcttccag actggatccg cgagcacaaa gaagaattta tggaaaaacg
     8221 ggtagtgacc tactgtactg gtggcattcg ttgcgagaag ttttctggtt ggcttgtccg
     8281 tgaaggattc aaagatgtcg gacagctgca tggcggtatt gctacttatg gtaaggatcc
     8341 tgaagtccaa ggagaattat gggacggcaa gatgtatgtg tttgatgaac ggatcgctgt
     8401 cgatatcaac caagtagata aacaaatcgt cggtcgcgac tggtttgacg gaacaccatg
     8461 tgaacgatat atcaattgta gcaatcctga gtgcaatcgt cagattcttg cttccattga
     8521 aaatgaagaa aaacatttag gagcatgtag catagagtgt gcgaaacatc cgcgcaatcg
     8581 ctatgttcaa gaccatggaa tctctccaga agaagcagaa ctgcggttta cagctttaat
     8641 taaatagaaa acaatagttt tgattgtcga ccatttccaa tatgcgctat actagcgttg
     8701 aggatggtga tccacatgaa atcattgatt tatgctattg gggatgtcca tggcaaacat
     8761 gagctttttg aaaaacttat tcactattat gatccaaaga tccatcagct tgttttatta
     8821 ggggatctca atgatcgagg tcctcgtaca aaagaatgtt ttttattagg aaagcaatta
     8881 gtcgaagaaa caggagccat ttacctccgg ggaaaccatg aagaatattt tttgcaattt
     8941 ctgcaatcac cggaggactg gtatgcgcct tatatgagaa atggtggaaa agaaacgttt
     9001 gaaagcctga ttcacccagg ggcatcggag gaatactccc caactgaaat ctcgatgatg
     9061 atccaatcga gatattcaga actgattaaa tttttagtcc agcgcccctt gtattatgaa
     9121 tgggaaaatt atctttttgt ccatgccggt gtggatttga caaagaaaga ctggcgtaag
     9181 acaagtctaa gagactttat ttggataagg gaaccatttc atgaagggaa aaacaataca
     9241 ggtaagacaa ttgtatttgg acatacgatt acaccaatgc tccatggaga tatgcagaca
     9301 acagacctct ggattcagga tcataaaatc ggaatggacg gtggggctgt attcggaggg
     9361 tcattgcatg gggttgtttt tgacaaggaa ggtattcttc aagatattga atatcagaac
     9421 aacgaaggac cttggcatgg aatctgacta atggaaagaa tgaaggagct gtgactttgt
     9481 tgcggctctt tttattttta tagagaatat tgtttctttc agtagtttaa cccaggataa
     9541 aaagtatgat agaataaaga agcaattttt agcagaaaag aggcatttta atgacggaaa
     9601 atttgaacca gacgatcatt cagcttgtac aaaaagaact ttcagactat cgtcctaagc
     9661 agttgactac tgtacttaat ttgttaaatg aaggaaacac tgttcctttt atcgcgcgtt
     9721 accgaaaaga aatgactgga agtcttgatg aagtccagat tcgtgaaatc gaagagcggt
     9781 atgcctatct tgaaaactta gaaaaacgta aaactgaagt gattcggcta atcgatgaac
     9841 aagggaaact gactcccgag ttagaagcag aaatcaccca agccgtgaaa atgcaacaag
     9901 tggaagattt gtatcgtcca tacaaacaaa aacgacggac aaaagcaacg attgctaaag
     9961 aaaaaggact tgaaccactc gcgaagtggc tgatgcagct tacagatgga gaggtgcaat
    10021 ctgaagcaga aaaatatatt gacaaagaaa aagaagtctc ttctgccgaa gaagcgttgc
    10081 acggtgctca tgagattatt gctgaacaag tcagcgataa tgcaaaattt cggacttgga
    10141 ttcggtctta tacgtataac aaaggtatgt atgtcagcaa tgtcaaggat gagcaagcag
    10201 atgaaaaagg cgtatatgaa atgtattatg actttgcaga acccgtccat aaaatggttt
    10261 ctcatcgaat attagctact aatcgtggag aaaaagaaga tattctgaaa gtattctttc
    10321 aagtagatga agcagcaatt ttagcttact tggatcgcca aatagtcaaa aatcctgcct
    10381 ctccttcttc ttcattcgtc agagaagcct atcaggatag ttataaacgc tttattcagc
    10441 cagcaatcga gcgagaactg cgcaatgaat tgactgaaaa ggcagatgag caagcaatcg
    10501 ctattttcgg cgaaaacctc cgtaatcttt tgttgcaacc gccactaaaa ggtaaagttg
    10561 tcctgggatt tgatccggct tatcgtactg gatgtaaact agcagtcgta gatgcgacag
    10621 ggaaagtttt agcaatcgaa gttatttatc ctcataaacc tgcagcgcaa gcaaaaagag
    10681 aagctgctgg accagctttt atccaattaa ttaataaata tcaagtagat atggtagcta
    10741 tcggaaatgg tacagccagt cgggaatctg aactttttgt ggctgagcag ttgaaatccg
    10801 ccgaccataa aacctattac gctatcgtca acgaagcagg cgcttctgtt tactccgcta
    10861 gcgaaattgc acgaaaagaa tttccgcatc tacaagttga agaaagaagt gctgtttcta
    10921 ttgcccgtcg cctgcaagat cctttagctg aattagtcaa aatcgatcct aaagctgtcg
    10981 gagtaggcca gtaccaacat gacgtttctc aaaaacgttt ggctgaacaa ttggattttg
    11041 ttgtggaaac agcggttaac caagtaggtg tggatgtgaa tacagcaagc ccgcagctct
    11101 tgcagcatat atcaggattg aataaaacaa ctgcccaaaa cattgtcatt tatcgtgaag
    11161 aaaacggaga attcaccgca cgaactcaat tgaaaaaagt gccacgctta ggaccaaaag
    11221 catatgaaca agcaatcgga ttcttgcgag tccctggtgg gaaaaacatt ttggacaaca
    11281 caggtatcca tcccgaaagt tattctattg caaaagaaat attaacttca gtccaacttt
    11341 cagaaaaaga gcttggtaca gaagaagcag tagaaaaatt gacacgatta tccgtagaga
    11401 aactggctga aagcctatca gtcggagaag aaacgttagc cgatatttta gctgggctga
    11461 cacaacctgg acgagatatg cgtgatgaga tgccagcgcc cttattacgt acagatgtat
    11521 tgagtatgga agacttgaaa ccagggatgg aattgactgg aacggtccgt aatgtcattg
    11581 acttcggcgc atttgttgat atcggtgtga agcaagacgg acttgtccat atatcgaaac
    11641 tcagcaaaaa attcgtcaag catcctacag atgtcgtgtc agtaggagat atcgtaacag
    11701 tttggataga acaagtcgat acgaaaaaag gcagaatcag tttgacgatg ctttcccctt
    11761 atgaagagta acaatgagga gtaacaaaaa ttgacacaag aagaactgca gcagttagta
    11821 gaagagatat cattaaaaag cttccagctt cctttcaagc accaagcaat gttcaatcca
    11881 cgcttacgta caactggcgg gcgctatcat ttaaacgatc atcatttaga ttttaatgcg
    11941 aagctatttg aagagtcaga cgaagagacg attgcaggaa tcatcaaaca tgaactttgc
    12001 cattatcatc ttcatctagc tggaagaggg tatcagcata aagatatgga tttcaagtat
    12061 cttttgaaaa aaacaggagg gttgcgctat gcaccagcct tgacgaagaa acagataaag
    12121 atggagtatt atcgatgcca agattgttct gccacgatct gccgcaagag aagaatcgat
    12181 gtacgcaaat atcgttgtgg aagatgtcat ggagaaatca aatggattga gacaaaaaga
    12241 gaagacttta atgaagcaca aaaaatcaga tgaaaggata gaaggccttt ggaaaaaagt
    12301 gagcagagga atctaaaaga ctggaatcag ctattaaatt ttttagccgc acctgaagag
    12361 ggaaattttg aggaactgac ggcacctaca cttattttag ctggaaactg tcttcctatc
    12421 ttagctgata aagcagcaca aatgtattta aatggccaag tagatcaatt gtttttggtc
    12481 ggaggggtgg ggcatgcgac tagaatcctt tatgagaatt ttgaaaagca aggatttcat
    12541 tttgaagatg gcatgagtga aagtgaaatc tgtcgtcagt atctaaaaga agtctatgat
    12601 ttgcccgata aggcttttct gatagaatcg aagtctacaa attctggaga gaacgccact
    12661 ttttcactgg agattcttca ttccttagga gctgtaccag aaaaaatctt attgatgaat
    12721 gatccgacac tgcaacgcag gactcgagca acttttgaga aagtatggca aaatgaacaa
    12781 actattttcg cgaatgctgt gccatttgtg ccggaaatcc ttcacttttc tgaagagatc
    12841 atctttacag caaaagaact gaatcatcaa tggcctaagg aatatttcca tgcgctagta
    12901 ttaggagaaa tggaacgtct ccatgacgat gagaacggct atggtccgaa aggaaaagat
    12961 tttattcctc acattgatat atcagaggaa gtttggtctt cttacaccag aattaaagag
    13021 tcaataaaaa cggatttttc tcgtacttga ccattttagc aagcgaatcc tcctttccaa
    13081 ataaatttgg ttgaaatttt caatcgttta taagataatg aggagcgtgt tgaatgtaat
    13141 agctaaaaga atggacgcta gataagagaa gaggattcaa atggaaaatc aaaataaatt
    13201 gtatcatttt gttggtatca aaggttcagg aatgagctcc ttggctcttg ttttacatga
    13261 acaaggtcta aatgtccaag gatcagacat tgaaaaatat ttctttacac aaagagattt
    13321 agaaaaagca aatatcacga ttctgccatt taacgcagat aatgtaaaac cagggatgac
    13381 gatcattgca ggaaatgcat ttcctgattc acatgaagaa atccagcgag caaaagaatt
    13441 aggtttggaa gtgattcgct atcacgactt tattggtcat tttatccaaa attatacgag
    13501 tatcgctgta acaggctctc acggaaaaac aagtacgact ggactgctct ctcatgtatt
    13561 gagtggtgtc cgtccaacaa gttatctaat tggagatgga acaggacatg gtgatccgca
    13621 agcggaattc ttttcatttg aggcctgtga atatcgccgt catttccttg cctattcacc
    13681 agactatgca atcatgacca atatcgattt tgatcatccg gattattata caagtatcga
    13741 cgatgtattt acagctttcc aaacaatggc tggacaagtg aaaaaagcta tttttgcgta
    13801 tggtgacgat gcttatttac gcaaattaaa agcaaatgtg ccgatttatt attatggtgt
    13861 cacagaaaac gatgatatcc aagcacgaaa catcgaacgc acaacttctg gttctgcttt
    13921 tgatgtctat catggggatg aatttgttgg gcacttcact gtaccagcat tcggtaaaca
    13981 caatatctta aatgctttag gtgttattgc agtagcttat ttcgaaaaac tggatctgaa
    14041 agaagttgca gaagaaatgc tgactttccc aggagtaaaa cgtcgattca gtgaaaagat
    14101 cgtagcagac atgaccgtag tcgatgatta tgcgcatcac cctgcagaga tcaaagcaac
    14161 gattgatggc gcacgccaaa aatatccaga taaagaaata attgcagttt tccaacccca
    14221 tacgtttacg cgtacgattg cattgatgga tgaatttgca gaagcacttg atttagctga
    14281 taaagtttac ttatgcgata tttttggttc ggctcgggaa gaacaaggaa acgttaagat
    14341 tgaagacctt ggagcgaaaa tcaaaaaagg tggagaagtc atcaaagaaa ataatgtatc
    14401 tccattgtta gattatcatg atgcagtagt aatcttcatg ggtgctggag atgttcaaaa
    14461 atttgaacaa gcgtatgaaa aacttttaag cagcacgacg aaaaatgtac tgtaatacaa
    14521 ttactgcagt gtccttataa aaatagaaag agggggagtg gcaaaaatct tgccaagccc
    14581 ccttttttgc aacttatgat aaacggtgtt caaaagcttg catctgcggt aataagccca
    14641 atcaaatcaa aaacatgaga agctgttttt tcttggctgt tcttattatt tgatgcagga
    14701 cagctttttc acgacctctg taaactatag gaatctgtta aatcaaggga gcagaacttg
    14761 ccttttacgc tacttttggt acaataatgt atgattaaaa ataaactgga gggtttctat
    14821 cttgaacata ggtaagccga gaaaactagg aaaaacaata aatgaaatcg aggaaggcga
    14881 ctcgttatcc ttaacagaat ctattgaaga taaccagctt ttgctgtatt tgggattgac
    14941 gaatgatgca aatcctttgt atatccaaca tgactttgca caaaaaacag aatatcaaca
    15001 gccaatcgtt ccatcagtga tgctgatggg gatcatcaca agtgcaattt caaagcatct
    15061 tcctggacca ggatcacatg ttgtcaattt ttctgtcaat tttattgaac cggttttcca
    15121 ttatgaaaca ctgacttttc agtttgaggt cataaaagta gataagatga aggatgtggt
    15181 gacgatttct gtagaagcgg agaatacgaa cgaagatcgt gtgttggatg ctgtcgttat
    15241 ggtccaaccg ccacaagttc ctgaattgga aatagcaatc gaaagtgaag gtgaattgaa
    15301 tgaataatcc aagtacatca atgtcaggta tcaatcgttt ttatgcaaaa gtttatggat
    15361 ttttaggtat gggacttgcc cttagtgcag tcacttctta cttggctctt tgggtatttc
    15421 ctttgcaatt agcaactttt atcaataatt ttccactagg ctttgtgggt ctgtggatcg
    15481 ttgaactgat tttagtaatg gttctaggcg taaaagcaca aaagaatccg agcttagcaa
    15541 tcggtggatt tattgcctac tctcttttga atggcataac attagcggtt actcttgcat
    15601 actacgatat tggaacagta acgcaagctt tcttgacagc tactttgatg ttcatgggga
    15661 tgtctgttgt cggagtaaca acaaaaagag atcttagcgg ggttggaaga gcaggacagg
    15721 cagctttaat cggagtgatc attgcactag tcttgaacat cttcattttg agaagtagcg
    15781 tagttgagct gttcatctcc ctactgttag tcgtcatctt tgcagggatt acagcttatg
    15841 ataaccaaag catccgtaat atctatgtcc agacaaacgg aacagcacat agtggaattg
    15901 ctgtattcat ggcattgaag ctgtatttgg actttatcaa cttgttcttg gcgtttctga
    15961 gaatctttgg acggaacaac taaaaaagaa aaaaattcat cagttggggc tataccgaat
    16021 tatttggtat ggtcccgctt tttcttttcg cattccgcag gattctttgt tagaatgaat
    16081 aagaacttta cagtaaggag ttaagaagat gacaaaaaat aaattattac tcgttgatgg
    16141 gaacagtgtg gcttttcgcg cattctttgc gcttcacaat tctcttgaac gtttcaaaaa
    16201 tagaaacggt cttcatacaa atgcaatcta tgcattcaat acgatgtttg aaaatgttat
    16261 gcaaaaagag caaccgactc atgttctggt tgcgttcgat gcaggaaaaa cgacttttcg
    16321 gacagaaatg tatgcggagt acaaaggtgg aagatccaaa acacctggcg aattcaaaga
    16381 acaaatgcca tatatacgtg atttgttgac aggactaggg gtacagtatt acgaattgcc
    16441 caattatgaa gcagatgata ttatcggtac tttggcagaa aaagtagaca aagatcaatt
    16501 tgatgtcgtc gtgctttcag gtgatcggga tttgactcaa ttagctagta aagaagtaaa
    16561 agtggatatc actgtaaaag gtgtcagcga tatcgaaagc tatacaccag aacatgtagc
    16621 ggaaaaatat gatgggttga cgccaaaaca gattattgac atgaagggac tagccggaga
    16681 tgcctctgac aatatccccg gtgtgactaa aatcggtgaa aaaacagcta tcaagctatt
    16741 gaaacaatat ggctcagtcg aaggaattta tgaacatatc gatgaaatga agcaaagcaa
    16801 aatgaaagaa aatctgataa atgacaaaga gcaggctttc ttatcgaaaa aattggcgac
    16861 catcaatact tcagcgcctg tggatgtctc tatcgactca ttgaagtatg aagggaaaaa
    16921 tctcgataaa ctcgttccgt tttataaaga aatggatttc aatcagttcc tttctaaatt
    16981 gaatatcgta gaagaagaag taaaaatgga tgatattttg tttgaagtcg ttcatgaatt
    17041 caaagaggaa atgtttacta cggatatggc gttgtacgtg gaaatgatgg gcgacaatta
    17101 tcatacagag gaaatcgtcg gagtagcgtg ggggacagaa aagaaaattt atgtcacgaa
    17161 cgatctatct gtattcgaaa accgagcgtt ccattcttgg atcactgatc cgactcggct
    17221 gaaaaaagta tatgatgcga aacgaactta tgttgcttta aaccgctata ctggaaaaac
    17281 gaaaggaatc gattttgatg tcttgctagg tgcatacctt ctcgatacga atgaaaaaag
    17341 cacagatatc gaaggaatag ctgctcatta cggatacaac gatatccaat cagatgaagc
    17401 tgtatacggc aaaggggcga aaaaaggttt accagaagaa gaggaactct tttttgctca
    17461 tttagcgcga aaagtagctg caatcaatgc gttgacacca aaattgagcc aagaactggc
    17521 agaaaaaaac caagaagatc ttttccggaa aatggaactg ccattgtcta tcattttggc
    17581 agagatggaa ataagcggga tcaaagtaga tgcgactcgc ttacaagaaa tgaagggcga
    17641 gttctctgct cgcttaagag agatcgaaca aaaaatctac gaagaagccg gcgaagaatt
    17701 caatttgaat tcaccaaagc aattaggagt gatcttattt gaaaaaatgg gattacccgt
    17761 tatcaaaaaa acaaaaactg gatattccac agccgtagat gtactggaac agcttcgtga
    17821 acaagcacca atcgtagagg atatcctcac ctatcgtcaa attgcaaaaa ttcaatctac
    17881 ctatgtcgag ggactgctga aagtgatcca atcagatgga aaagtgcata cccgttatgt
    17941 ccagacattg acccaaacag gacgattaag ctctgttgat cctaatttgc agaacatccc
    18001 gattcgatta gatgaaggga gaaagatacg tcaggcattt gttcctagag aaaaagactg
    18061 gctgatttat tcttctgact attcacaaat cgagttacgc gtacttgcgc atatctcaaa
    18121 tgatgaacat ttgaaagctg ccttcctaga aggtcaagat atccatgcca acacagcgat
    18181 gcgtgtattt ggaatcgaaa aggcagagga tgtcacacca aatatgcgta ggcaggcaaa
    18241 agcagtgaat tttggtatcg tgtatggcat ctctgattac ggactttcac aaaatctagg
    18301 aatcagccga aaagcagcac agcaatacat cgatacgtac tttgaaaaat acccaggtgt
    18361 caaagaatat atggaaagaa tcgtacgcga agcaaaagac caaggctacg tcgaaacgct
    18421 gtatcaccgc cgtcgatatc tgccagacat caattcaaga aattacaata tccgttcttt
    18481 tgcggaacga acagctatca atacaccaat ccaaggaagt gctgctgata ttttgaaaat
    18541 cgcgatgatc gaattggata aacgattaaa agaaacaggt cttcaagcta cgatgctgtt
    18601 acaagttcat gatgaacttg tcttcgaggt accggaaaaa gaacttgaat cattagataa
    18661 attggtgaag gaagttatgg aacaagctgt ttctcttcat gtaccactga tcactgacag
    18721 cagttgggga aaaacgtggt acgaagcaaa ataaacattg tttaagacag agcaatataa
    18781 tgaaggtgaa aagaaaaaat gccagaatta ccagaagtag aaaccgttcg taaaggattg
    18841 gaacggttag tagtaggaaa aacgattcaa aaagttcagg tcttgtggcc caagatcatc
    18901 gagcaaccag agacacccat ctttgaggct tctcttgttg gcgaaaccat ccaatccatt
    18961 ggacgtagag ggaagttttt aatttttcat ctcgatcatt gtgaactgat ctcacacttg
    19021 cgaatggaag gaaaatatca atttacgaaa gaaaatacac caatcgataa acatacgcat
    19081 gtacttttct tttttgagga tggaagccaa cttcgctaca acgatgtacg aaaatttggt
    19141 cgtatgacaa tcgtcgaaaa aggagcaagt gcaacttacc gaggaatcat gaaattggga
    19201 cctgagcctt tgccagatag cttcttatta gcagatttcg caaatggact gaaaaagtcc
    19261 cacaaagcaa tcaagccatt gctgctagac caacgtctag tgactggtct aggtaatatt
    19321 tatgtagatg aagcattatg ggaagcgaag atccatccag aacaaccggc gaacacactg
    19381 cgccccaaag aggtagaaca attacgtcta tcgatcattg atgttttgga tcgtgcaata
    19441 gaagccggag gtaccacgat tcgcagttat ttgaatgcat taggagaatc aggcggattt
    19501 caagtagcac tgcatgtcta tcagcaaact ggcaaaccat gtatacgttg tggaacgccg
    19561 atcgtgaaaa caaaagtggc acaaaggggg acgcactatt gtccgaattg tcaaaggcta
    19621 aaaatcgcaa agtaggattt gttttaggtt tgacgggtgg aatagctacc ggtaaaagta
    19681 cggcagccaa agtctttcag tctcatggat ttcctttgat cgatggcgat gtgattgcca
    19741 gagaaactgt tgagcctgga acgcttgctc tgaggaaaat tgtatcagtg tttggccaag
    19801 aggtattgca gcctgatggg caactagatc gagggaaact tgggatgatt gtttttccat
    19861 caaaggaaaa acggcaaaaa cttgatcagc tattagatcc gtttatccga aaagcgataa
    19921 aagatcaaat cgcctctcta agttccaatc atcctttagt aattgtagat atcccattgc
    19981 tctacgaagg tcattatgac cactatatgg atgcagtagc tgtcgtttat actacgccag
    20041 aaactcagct tcagcgtttg atgaaacgta atcaactgac gctagaacaa gcgcagcaga
    20101 gaattagtag tcaacttcca atcgaagaaa aaaagcagcg tgcggatatt ttgttcgaca
    20161 acaatggaac aaaagaaaaa cttgtgggac aaatcgaaaa ttggcttttt gctaatcgct
    20221 ttctttgagt atctgtgtca attttgctat cctgagtata tagaaagtga gggatagcga
    20281 tgggatcatt aaaaaatcaa aaggataaag tcgtaggtaa cgcaaaagaa aaaatcggcg
    20341 agatgctcaa taaagaaaat ctaatcgatg aaggtcgtac gcaatcagaa acagcagaag
    20401 aaaaagaaaa gcagtaccaa gcaagaatcg atgaaataga agaccagcag gcagaagaag
    20461 aagcgaagcg gcaagatttg actcaagaac gaaaaaatcc gacacctgat cgtagtaaaa
    20521 caccgctccc agaaagtgac cacattcccg atgagcaaaa aatcgaggaa cagcgaatga
    20581 aacactatac aggaatcgaa gagaagttat aaaatagtaa aaaatcgaga tgtgacttga
    20641 agcagatgag aaggtgttca tctgcttcaa gttttttgtg ctagatgact aaatcagctt
    20701 ttgtgtaagt tatttctcag taatcaaggt gatcagtatt ggaaaattga aatttagtaa
    20761 ggacagggga cacaattacg catgaaagta gaaaaaattg ttcttatatc cgtgtttttt
    20821 gggtaggaca gtcgcgtttg tgatttgttt cgtgttataa tacaactagt tgtaaagaaa
    20881 gacacatcct ttatataaaa tgcgatgcaa gaggtgagac aaaatgagat gtcccagatg
    20941 tcaacataat aattcacgag taatcgacag ccgtcaagca gatgatggac gtgcgatccg
    21001 tcgccgtcgc gaatgtgaga attgttcgtt ccgattcact acatttgaac gaattgaagc
    21061 tgctccgtta ttagtaatca agaaaaacgg ggaacgcgaa gagttcaatc gcgataaaat
    21121 tttaagagga ctcatccgtt cagcagaaaa aagacctgta gctatggaac aaatggaaca
    21181 aatcgtcaac catgtcgaaa accgcgtacg cagtctcgga gaaaacgaag tatcgacgaa
    21241 tctaattggt gaatatgtga tggaagattt agttgattta gacgaaatcg cttatattcg
    21301 ttttgcaagt gtttatcgcc agttcaaaga tatgtctgtt ttcttaaaag agttgcaaga
    21361 tatcgtggat aaagcaaaag aaaataataa agagaataat aaagaaaaca attaagaaac
    21421 aaaaaggagg catttccttg gaaaatgctt ggaaagagct tcaaccaaaa aatatttacc
    21481 aaggaagaaa agcggctccg ctgagagaag acagcaagaa cgccttgctg tatctttatc
    21541 agccactaat cggcggagaa gccttgagta tctatttcac cctgttatca gaaatctctc
    21601 aagaatctgg caaaggtcct gaagggatgc atgcagatct gcttagtagt ctaggctgcg
    21661 gtatcccgca attttatgat gcaagaaaaa agctagaagg aatcggctta ctggatgttt
    21721 attttaaaga agatagtgat ctgggacctc gttttattta tgagctgtta gaaccactca
    21781 ttcctaaagc atttttcaaa gactctgttc tggcttttct tcttctggaa aaagttggtg
    21841 aacgacgatt ccagaagctt acccaattct ttgaacctaa aaggcataca gcagagggat
    21901 atcaaaaaat cacaaaaaaa tttttagatg tttatcgctt caatgaagca gtgtacgctg
    21961 ctaatcaaga aaacatcgaa tcgctgaata aatcttttga agaatctgaa tcagtagcat
    22021 tacttgaagc agattcgaca ttcgattggc agtttttgaa tgattggctg gcaaaaaaac
    22081 atattgcaag tcttgatgat ccaatcatca aacaagtcga catgtatcat cagctttacg
    22141 gtttcgatga attgacactt ggcgaactga tcagtcaatc ttatgacttt agtgaacaaa
    22201 aagtatctga aaaagaactg caaaaagcca ttttggcaat gcagagacgt ccggtgaagc
    22261 aaacaaaaga aacagttgcg gtttctgaaa acagtaatga accctcgtca aacgaacatt
    22321 tgacaaacgc agcgcttcag ctgataaaag aagcacagag cattccaccg atgaaatatc
    22381 tggaagcaat caagtcagaa aaaggcggat atgtttctaa acaagaaaac tggttactgc
    22441 aagatcttgt ttctcaatca ggtctttcca gtagcgtgat caatgttttg ctgaattatg
    22501 tactagtgat caagaataat gctagcttaa atgccagtta tgtaaataca gtggcaaatg
    22561 aatgggcaca aaagaaaatc gccacagcag aagaagcgat ccagcacatt cggcaaataa
    22621 gtaatcaagc aaaacaatca aaacaaaaga agcaaacaaa ttatcaaact ggcaagcgaa
    22681 atgttcgacg ggaaaagctt cctgattggg tcaaccagcc aaaagatgaa aaacagatca
    22741 gcagcgagaa aaaagcagag attgaccgcc gttttaaaga atacctggca aaaaaggagg
    22801 gtgatatcta gtggaagacg taggcaaaga attgaagagg atcatcagca aacgcaatta
    22861 tcaagaaaag tatgctgaaa tgatcgctga agtcttaaat gatgcggatg ttcaagcttt
    22921 tctaagcgca caccaagaag aattgacaca agcggatatt gaaaaaagtt acgctaaatt
    22981 gtatgaattc gtgcaagaaa agcgtaaata tcaaaaaaac gatccaacga tgatcgctcc
    23041 cggctatgag ccgaagctga cgttgaattt tcattatgtg gatgtcactt atgtaccaac
    23101 ggaagcattg ttagcacggc aaagacaaga agaaatcaga agccgagtaa aagcaatgga
    23161 tatgccaaaa gatattcaat cggcttcttt tgaaaacttt gaacgtacag atggacgagg
    23221 atatgcgagc ttagaagccc tagattttgt agagaaatat actgctgatc caaaagggta
    23281 tcataaagga ctgtatctag taggaagttt tggtattgga aaaacatatt tattaggagc
    23341 aacagcgaga gaattagcga ttgctggatt caccacgacg ctagtccatt ttccgacatt
    23401 tgccgttgag atgaaacagg caatcggaaa agatcaagtg gcagaaaagt tagatgcagt
    23461 gaaacgttca cctattttga tgatcgatga cattggtgca gatgcgatga gtagttggat
    23521 acgagacgaa gtattcggtg ttatccttca atatcggatg caggaacagt taccaacctt
    23581 cttctcatcg aattttacaa tggatgaatt ggaacagcat ttgtccgtca ctcaacgtgg
    23641 agatgaagag ccgctaaaag cgaaacggat aatggaacgc atacgttatt tgacaaaaga
    23701 aatcgaaatg actggtcgca accgacgcaa tggttgaaca gttgacaagt ccaactgaca
    23761 tttgacctag tagtaaaagc aggtcagcga atcattatat ggacagaaat gaccggagac
    23821 aatcaccgca acaagtaatg cagaaactta aacggaggat tttttatgag ggctattttt
    23881 tctagtatat ctgtagttgg tctatttttt cttttagcag gctgcggtag cgcagatagc
    23941 aacacaggtt ctgttgattc gattgatgcg ggtcaaacag taaaaacaag cagtacgaaa
    24001 acggagagac aaagcatatt tgtcggaacg attgaaagta tcactcaagt agaagcagaa
    24061 gaagaaacgc ttcagttgaa actcgtcgat gtcatggcca aagaagatcc agcgaatatg
    24121 ggaacgtcat tcagcaatga cggagtgatc ttaaatgcta ctgctgaaaa aataagcgtg
    24181 gacataaatg aattgaaaat cggtgacaaa gtccaattta cattagttga gatgccgatc
    24241 atgacgatgt ctatcccacc gcaagttcct ggagattcga ttattgatgt ttcattgaac
    24301 aaataaccaa taacattttt tcaaaaataa gaaaatgttg ttttatagaa cgaaaaggta
    24361 tattttttaa ggagatatgg gaattt
//
LOCUS       DAZKOR010000039        24258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000039 DAZKOR010000000
VERSION     DAZKOR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.039"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG8ZM_001464"
     CDS             complement(45..1340)
                     /gene="purB"
                     /locus_tag="ACG8ZM_001464"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0492429.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG8ZM_001465"
     CDS             complement(1353..2477)
                     /gene="purK"
                     /locus_tag="ACG8ZM_001465"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0492430.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG8ZM_001466"
     CDS             complement(2470..2958)
                     /gene="purE"
                     /locus_tag="ACG8ZM_001466"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0492431.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3374..4681)
                     /locus_tag="ACG8ZM_001467"
     CDS             complement(3374..4681)
                     /locus_tag="ACG8ZM_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0492432.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4684..5265)
                     /locus_tag="ACG8ZM_001468"
     CDS             complement(4684..5265)
                     /locus_tag="ACG8ZM_001468"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0492433.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5381..5478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5837..7129)
                     /locus_tag="ACG8ZM_001469"
     CDS             complement(5837..7129)
                     /locus_tag="ACG8ZM_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0492434.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7126..7749)
                     /locus_tag="ACG8ZM_001470"
     CDS             complement(7126..7749)
                     /locus_tag="ACG8ZM_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492435.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7752..8588)
                     /locus_tag="ACG8ZM_001471"
     CDS             complement(7752..8588)
                     /locus_tag="ACG8ZM_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0492436.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8605..9492)
                     /locus_tag="ACG8ZM_001472"
     CDS             complement(8605..9492)
                     /locus_tag="ACG8ZM_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0492437.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9508..10791)
                     /locus_tag="ACG8ZM_001473"
     CDS             complement(9508..10791)
                     /locus_tag="ACG8ZM_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0492438.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11474..12157)
                     /locus_tag="ACG8ZM_001474"
     CDS             complement(11474..12157)
                     /locus_tag="ACG8ZM_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0492439.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12175..12792)
                     /locus_tag="ACG8ZM_001475"
     CDS             complement(12175..12792)
                     /locus_tag="ACG8ZM_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0492440.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12802..13626)
                     /locus_tag="ACG8ZM_001476"
     CDS             complement(12802..13626)
                     /locus_tag="ACG8ZM_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0492441.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13619..14179)
                     /locus_tag="ACG8ZM_001477"
     CDS             complement(13619..14179)
                     /locus_tag="ACG8ZM_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492442.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14353..15309
                     /locus_tag="ACG8ZM_001478"
     CDS             14353..15309
                     /locus_tag="ACG8ZM_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0492443.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15614..16570)
                     /locus_tag="ACG8ZM_001479"
     CDS             complement(15614..16570)
                     /locus_tag="ACG8ZM_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0492444.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16674..17276
                     /locus_tag="ACG8ZM_001480"
     CDS             16674..17276
                     /locus_tag="ACG8ZM_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0492445.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17364..18863)
                     /locus_tag="ACG8ZM_001481"
     CDS             complement(17364..18863)
                     /locus_tag="ACG8ZM_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0492446.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18879..19403)
                     /locus_tag="ACG8ZM_001482"
     CDS             complement(18879..19403)
                     /locus_tag="ACG8ZM_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0492447.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19701..20450
                     /locus_tag="ACG8ZM_001483"
     CDS             19701..20450
                     /locus_tag="ACG8ZM_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0492448.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20441..21226
                     /locus_tag="ACG8ZM_001484"
     CDS             20441..21226
                     /locus_tag="ACG8ZM_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0492449.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21195..21911
                     /locus_tag="ACG8ZM_001485"
     CDS             21195..21911
                     /locus_tag="ACG8ZM_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0492450.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22013..23032)
                     /locus_tag="ACG8ZM_001486"
     CDS             complement(22013..23032)
                     /locus_tag="ACG8ZM_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0492451.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23051..24052)
                     /locus_tag="ACG8ZM_001487"
     CDS             complement(23051..24052)
                     /locus_tag="ACG8ZM_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0492452.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7623 t
ORIGIN      
        1 tttttgctat tctattttaa ttgaattttg caactcactc ttctttattc caaacctact
       61 cgtttgaata tcacgtcaac gtttttcaaa tgatgatgat aatcaaaggc atcgtctatt
      121 tcttctttag ataataccga tgtgatggtt ggatcttctt ctaacagcgg gcggaactgc
      181 acttggttgt cccaagcgta cgcagttttt ggctgaacaa gatcataagc ttcttctctt
      241 gtcattccct tatcgatcaa tttcagcata acgcgctggc tataaatcaa accaaatgtt
      301 gcagacatat tccgcttcat gttttcagga aagaccgtta gatttttaac gatattgcca
      361 aaacgattta acatgtaatt caataaaatc gtggtatcag gcaagatgat tcgttctgct
      421 gaagaatgag aaatatctct ttcatgccat aatgtgacat tttcataggc agtgaccatg
      481 tgtccgcgca ccactctggc tagaccagcc atattttctg aaccgatcgg attccttttg
      541 tgcggcatag cagaagaacc tttttgacct ttcgcaaaaa attcttctac ttcacgtgtt
      601 tcggattttt gcaatccgcg tatctctgtt gcaaaacgtt caatacttgt agcaataagc
      661 gccatcgctg agaaatattc agcatgtaga tcccgaggga gaacttgagt agagatttct
      721 tgtggacgga tccccaattg tttacataca tactcttcaa taaatggcgg gatatttgcg
      781 aacgttccca ctgctcctga gatttttcct gcttctactc cttttgccgc atgttcaaat
      841 cgttcgatgt tccgtttcat ttctgaatac catgtggcaa gtttcaatcc aaaagtagtt
      901 ggttcagcat ggacaccgtg tgtccgaccc atcatcactg tatatttgtg ttcttttgct
      961 ttttcgccaa tgatttccgt aaaacgtttc aagtcttccc gtaaaatatc atttgcttgc
     1021 ttgatgagat agccataagc agtatctacg acatccgtac ttgttagacc ataatggacc
     1081 cattttcttt cttctcccaa tgtttcagaa accgctcgtg taaacgcaac gacatcatgg
     1141 cgtgttaatt tttcgatttc caagatccga tcgatatcaa atgatgcatg ctcacgaatt
     1201 tttttcacat cttcttgcgg gatttcacca agttctgccc aagcttcatc tgctaaaatc
     1261 tcgacttcga gccacgcacg gtatttgttc tcttccgtcc agatattccc catttcaggg
     1321 cgtgtataac gttctaacat gttttttctc ctctaatccc aaatattcgt ttgtttgatt
     1381 tcttccagcg tttgctggat gtcttctgtc aagatagtga tatgtcccat cttgcgacct
     1441 ttttttgctt gtttttttcc atagaaatgg aattgccagt ctggcttatt ctcaatcaag
     1501 cgatagcttt ccagcagttg ttctcccaaa acattgacca ttactgcatt gcttatcaaa
     1561 cgtacttttt ctcctaatgg ccaaccacaa atacctcgaa tatgggcatc gaactgactc
     1621 atagaacaag cttcaataga ataatgtcca gaattgtgag gacgaggagc taattcattc
     1681 acatatagcc cgcctgtttt tgttaaaaac atttctaccc ctaaaacacc tgaaaggctc
     1741 actgcttctg caatcacacg ggcaatacgc tcagcctcct cgatcacatc ctgatcaatc
     1801 gcggctggag caatcgtttc atgaagaata ttgttgtggt ggatgttttc cacgaccgga
     1861 aaagtcgtga aatctccttg accatttcct gccaccatga tcgaaatctc tttttcaaac
     1921 gggatccatg cttctaaaac acaggttcct tctcttaaaa ggtccatggc aggagctaga
     1981 tccgcccgac ttttcaaaac atactgcccc ttgccatcat agccgcctcg agtggttttc
     2041 aaaacacaag gatagccgat cccgtcaatc gcatcttgga tatccgtcgg actgacgatc
     2101 gttgcataag gagcaatcac gatgttattc gtttccaaaa aagatttttc taacaggcgg
     2161 tcttgcgtaa tcgcaagcaa gtctgtccct tgaggaataa atgacatggg taaaatcgca
     2221 tttaacgcct ctacactgac gttttcgaat tcataggtaa tgacatctgt tcttctggca
     2281 agctctttca gcgcaaaggt gtcatcatag acactttcaa tgtgccaatc tgcaacttgt
     2341 gcggctggac aatcgctcac aggatccagc acacctactt taaagcccat ttcttttgca
     2401 ctaatcgcca tcatacgacc taattgaccg ccaccgacga tcccgatcgt tgaaccaggc
     2461 aacaaaggtt taaccaagtt gatcactact ttctataaca gttttagcca acattgaacg
     2521 tctttcagcc aatttagcct ccaattctaa atcatacatg gaaagcatct gtatcgcaag
     2581 tagtccagca ttaattgctc ccgcttttcc gattgctgta gtagctacag gtactcctcc
     2641 aggcatttgg acaatcgaaa gaagcgaatc caaaccattt aatgttcgtg attggaccgg
     2701 tacaccgata accggcaaag ttgtttttga tgcgaccata cccggcagat gcgcggctcc
     2761 ccctgctcct gcaataatga ttttgattcc tttttttcga gcgttttgtg catattcaaa
     2821 catcaaatct ggggttcgat gagcagaaac gacctttttt tcataagaca caccgaattc
     2881 atccaatatt tgacatgctt ctttcattgt ttcccaatct gaagtactcc ccataatcac
     2941 agagattttt gctgtcattg tttttcctcc cagtttttct acattcatat tttaacaatc
     3001 actttttttg atgtcaaaga aatttcgaat gttaacaaag aaaaacattt tattgttcgt
     3061 gtttattaaa gataaaaaaa aggaatgtga caaaagttag gtcacattcc tttttatgga
     3121 taaacggtgt tctcctgcca actcctcggt cacaaggaac aatattcaac aaacatgaga
     3181 agcttttttt tgaaattgtt ccttgtgact cggagatgaa cggcaggctc acgacctctt
     3241 ccttcaacgg tgttcaatca gctgaccctc ggtattagct caaacaaacg gcaaaatacg
     3301 aggaactatt tttccgtttg tttctctaac aatcaggtca aaacgctgat ttcacaacct
     3361 ttcttttgct ctattacttc ttgaaggctt cttccaatat ttcttcatct tttttcaatc
     3421 cattaaagat caaattcaat atgatagccg taatgctact cattacgatg ccatttccag
     3481 tgaacatttg caatgtttgc ggcatttgac tgaataatgt cggcgtaaca ttgaaaccca
     3541 gcccaaatcc aacagaaaca gcaacaatca atagattttt atcattgctg aaatcaactt
     3601 ccaacagcat acggattcct tggactgcca ccatcccgaa catgactaac attcctccac
     3661 caagtaccgg ctcagggatg atttgtgcta acgcaccgat tttagggaat aatcctaaaa
     3721 caatcaagaa gaatgcagag aaatagatgg gttttcttgt tttgattccc gaaagttgaa
     3781 ccaatccgac attttgagaa aagccagtat aagggaatgt attgaaaata ccgcctaaga
     3841 taacggcaag tccttctgca cgatatccct ttttcaattc ttcttctcca acatttttcc
     3901 cagtaatgtc accaagtgca aaatatacac ctgttgattc taccatgctg accatggaaa
     3961 tgatgatcat tagtaaaatc gaccatacgt cgaaagttgg tttaccaaaa taaaagggtt
     4021 gtggaacatg gaacaacggc gcatctccaa tcgcgctcag atcaatcatt cccatgaaag
     4081 cagctaagat gcttcctccg actaaaccaa tcaataccgc gattgaacga atgaaccctt
     4141 ttgccagcat ttgcacgcct acaattagga gaatcgtcac aaatgcaagt agcagatttg
     4201 ttttgtcacc aaaatcagct gctgttgcat tacctccacc cattttctcg attgctacag
     4261 gaatcaaagt caaaccaata acggtgataa ccgttcctgt tactatagga gggaatagtt
     4321 ttttaatttt agaaaagaca ccagaaacta acacaacaaa gatcccagaa gcgataatag
     4381 atccgtagat agctcctact ccttcattgg ttccgatcaa gatcagtgga gctactgctt
     4441 ggatcgcaca tcctaaaacg acaggcaagc caattccgaa aaatcggtta actgtcaatt
     4501 gcaataatgt cgctaatcca cacataaaga tatcaattga aatcaaataa gtcatctgtt
     4561 cttgattaaa gcctagtcct gtaccgatca aaagaggcac cgctactgct cctgcataca
     4621 tcgctagcag atgctgcagc cctaaaaccg cagcttttcc attttgtgtt tctttttcca
     4681 cgattatgcg tcctcctcaa gaaactcaac ttgtccattc gtcaaggaag caatacgcgc
     4741 aagagaaacg actctcaatc ccatttcttc aagtaactga cgtccatctt ggaaagactt
     4801 ttcgatgacg atcccgattc cttctacttg cgctccagct tgctggcata attcgatcaa
     4861 tccttttgcc gcttgtccgt ttgccaagaa atcgtcgata atcaatactt tgtcttctga
     4921 agataaaaat ttacgagaaa tagaaatcgt acttgtcact tgtttcgtaa aagaataaac
     4981 agaagctgtc aataattctt catccatcgt caaacttttt gcttttctag cgaagatcat
     5041 tggaacatct aattctcttg cagcaaataa agctggtgcg atcccagaag cttcaatggt
     5101 cacaactttc gtgattcctt gttctttaaa tacttctgca aaccgttttc ccatttgttc
     5161 catcaataca gggtctactt ggtgagtgac gaagctgtcg acttttaata cgccctcgcc
     5221 taatatacgt ccgtcttttt gaatacgttc aactaattct ttcactttat ttgctccttt
     5281 atcatttatt aataccgaaa taaccgcttt tgtgcaaaaa cctactatgt ttctgttaaa
     5341 gaaaaacacc ctccacttta tcataagaaa agaagggtga aatagccaat cttttactta
     5401 tagtccagca tttacggtac tggctagaaa ctgtcatcca tattatgacg atatataagt
     5461 aggtatgtaa aactaataat agtgtaaatt cattttcttg caactgtttt gcacgtgaag
     5521 gcagtttgcc atatttgaat aaaaaatgtt gtttcaaata ttgaaaacag tctaacacta
     5581 ctttctgaaa ctgacaacgt ggtttactta tttttttgta aaatctaatg atttttaatg
     5641 cattattcct ttttgttcgg gtttaaatag ttatttttat tttctcctat ctaaaaaagc
     5701 agcccacata aaaagaaggc atgacaaaaa gcatgtcata gccccttttt ccgaataaat
     5761 agtgttcaaa agacagccta tcggcaataa ggcaagattt tataaactac gggaagtaat
     5821 ttccgtaaat ctattcttat tcttttaagc tgacaagctc gtcaatcact actgcttttc
     5881 ctgaagcaat agaatgattt gctgcgattc ctgtcaaaat cgacaaaata ccatctacgt
     5941 gagttgctgc ccgattccac ggatcttctt ctttttcttc accaaataga tcatctaata
     6001 aacgcggatc tcctccgcca tgtccgcccg cttcttgttc gacgattacc tcatatggct
     6061 caccgaacat cggatagaca tggatggact gaagaacggc tgctccttca tcctttttat
     6121 ctccacctgc attgatgtaa gacttttcga tcactttcat ttcgatccgt cctttgctgc
     6181 cattgaaaga aacattgaat ccttcccacg gcatatacgc gtttagcgaa tacgtcaaaa
     6241 tcgcaccagt ttcatatttg accgttacgc ctagtgtatc ttcaatagag atatcatcgg
     6301 aaaatacact gcggtcacga atatatccac tgtctttttc tgcttctaaa tacatttctc
     6361 gcaattcctc attggctgac aggtctatag caaatggatc agctttagct agttcgcttc
     6421 catagcatcg actgtaaaat atttctactc cgcgtttctc cgcattatgc atcccataaa
     6481 agtttaatcc accgaatgca aatacctttt ggggacggct gttcaaccaa aaattcacta
     6541 gatcaaaatg atgggtagat ttatgaacaa gaagcccgcc gctattgtgc ttatttcgat
     6601 gccatcttct gaaataatct gccccgtgtt gcgtatttaa cagccattcg aagtggatag
     6661 aaaacacatc accaatcaca tcactttcta tcagttcctt tatcttcgtg tgatacggtg
     6721 cataacgata attaaaagtg actcgcaggc ttcttcccgt ctgtttttgg accgttatta
     6781 tttcttgagc tttttcctca tcgatagtca gaggtttttc gcaaataaca tcatatcctt
     6841 ttttcattgc acgaatgata taatcatgat gcgttcgatc tactgacgta ataatgactt
     6901 cagccggccg tacgtcatcc agtagttgct caaattgtga tggcatacat gtctttactt
     6961 ttttctgtcc atacttgttc acaagtatcc gatttgcata atccatcctt gtttgagata
     7021 catcacaaaa accgactagt tcacaagttt gatgatacgt agaggcaatt gcctcataaa
     7081 aaaaacgcgc tctcccacca agtccgactt gtacatattt tttcttcatt taaagtcatt
     7141 ctccttttct tttaagcaaa atactgtgac aaatggcagc cctggataca gtaatgcagt
     7201 gagtaaaaaa tcatgctgta tacaccagaa cagtccatac aaaaggcata aataccacag
     7261 aaaccccttg acgttttttg acaacagcca gatattcctt ttgatcaaat aaacaagact
     7321 atctgcttta tcttctttgc agatatcaat tagcgtacta aaaaaactat gggatacaaa
     7381 aagcaacaga cacaatagaa aaggaatcag ataccaagct ccaatctttc ccactaataa
     7441 ataccaatca actgcaaaaa taatgacaaa cagctgaaac aacgtaatga tgaaacacat
     7501 ccgtttgatt tttcctttcc tccaacatac ctttttttct gccaagtaat ggacagatag
     7561 ataataagaa ggatacatca tcaaaaaata aatcagatag acaacacgag cagacagcac
     7621 taatcctaaa gtgaacaaac atcctagaac taaagcgaaa agagcattac tgataagact
     7681 ggttaaaaaa atccgacaga catagtagtt gagcttgtaa agaaacatct cttctattgg
     7741 ctttttcata ttcagccctt cagaccgctt gtactgatcc cctggacaag atatttattg
     7801 aagaataaga aaatcagaaa aaccggtagc agcgacaacg tagacatcgc aaacatactc
     7861 gcgtaagtcg tactagatcc agcgtctgca aataatttga tggcaatcga taccgtgtaa
     7921 gatctcggct tgtttaaata aatcaatggt cccatgaagt cgtcccattt ccaataaaac
     7981 tggatgatga tcgtcgtgat gatcgaaggt ttcagcaaag gaaaaataat tttggtaaag
     8041 acactatact tgctgcatcc gtcaataatc gctgattcat ctaactcttt ggggatgctg
     8101 accatgaatt gcatgatctg gtaaatgaaa aatgcttgtc caaaaaaatg tggtaaaatc
     8161 agcggcacat acgtcccaac caaattcagc cgattgtaaa taataaactg cggaatcaaa
     8221 atcacttgtg caggaatcat catcgttact aacatgacga cgaaccagaa tctttttcct
     8281 ttgaaatcga ttctcgataa tgcgtaagca atacaagtcg agctcagaac tgtccctatc
     8341 gtcgaaactg cagagatgac aaacgaattc ctaaagaaag taaaaaatga aattccagca
     8401 aaccccgccc atccttttgc gtaattttcc cattgaaaag tctctggaat caaattcagc
     8461 gaaccactca taatctcttg atccgtttta aaagaacctg cgatcatcca taagaccgga
     8521 taaatcatga ctagacctaa tagcaaaata aaaccgtatt gaaaccattt cgttactgta
     8581 ttattcattt cttcctcctc tcattcacac aacgacccat ttttttgatg atttgaaaat
     8641 cagaaaggtg acagctccca taatcaacag catgatccac gaaatagcac tagcataccc
     8701 catagaatta aaagaaaaag cttggttata cacataaaga gcataaaagt tcgtggcatc
     8761 attcggacca ccttgggtaa tgataaatgc ctgcgtaaaa gtcataaacg ccgcgattgt
     8821 ctgcataacc aagttataca aaatgatcgg cgaaagaaga ggtaacgtga tcttgtaaaa
     8881 cgtctgccat ttgttcgctc catcgatctc tgccgcttca taatacgagc ctgggatctc
     8941 tttgatacct gcagcaaaga tgatcattgc cgaaccgaat tgccaagcag acatcaaaac
     9001 aagagggatc atgaccagtt tttgatcgct aaaccaggaa agcgcaggtg cgcctaatcg
     9061 ttccaggaaa atattcacta acccgttttt agaaaacaac tcccgccaaa ctagagcgat
     9121 agaaatactg ccaccgatca aactaggcac ataatacaaa gaacgaaaga aagtcgttcc
     9181 tttgatattc ctcgttaaaa gataagcaat caatagacca aagatcagtt tcacaggtac
     9241 agatgcaaat acatagagaa aagtgactcg gatggagttc aaaaaacggg catcactcag
     9301 catctttata taattatcca agccgatcca tgagactgga tttcccaaac gataatcggt
     9361 gaacgaataa taaagtgaca agatcatcgg tatcactgtt atcaatagaa atcctaggat
     9421 gaatggagaa gcaaatacgt aaccgaccac attgtcttga tccaagaacc gttttagttt
     9481 ttcttttttc atgccttcct cctgctgcta tcctagaatg tttgacgcaa actcataaag
     9541 actctctgct gcttcttctg ctgttacttc ttcataaatg actttttcaa ctaaaagatt
     9601 gtattggtct tgaatttccg gattcaaagc actatcaaag acattcactt cgccgttgtc
     9661 catatcggaa atcatttcta aataatcaaa tgtcgcttgc aaagttgcat catctgcacg
     9721 agcgagctgt tctcgaatat tcgacatgat tggtacacca cgttctcctt ttaaaatttc
     9781 attggcttct tcactgtttt gaaagaaatc aataaactta gcagcttctt ctttatgggc
     9841 tgagcttttt gccatactca gcatttgcga agaacgtaaa gggataccag attctccatc
     9901 tgcttttctg cgaggtggat taatcaactg aatctccata tcttcaggag cccctgacaa
     9961 tatttctgtc aactggttac ttgatagaaa caccattgcc gcttctctat ccctaaccgc
    10021 ctgaaaagac agatccgtcg tacttgcagc tccaccacct ggttctggat gggccccttt
    10081 gtctaccaga tttttacgca gctggaaaaa atctgttaga tagtctggat tttcaaatcc
    10141 caagctgtta gatagatctt cccgattata aaaattcaat cctttctcaa cttgtggtat
    10201 ttgaataata cagccagcta taaaatcttc gaatcttgaa atagcataga ttcctaattc
    10261 ttcagtgatt tgatagctga tttgttcaaa ttcatcccat gtccaatttg aagctggttc
    10321 agggatattt aattgttcaa atatattttt attgtaagca atcgcaaacg aattcacccc
    10381 gttagacagt ccaactaatt gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc
    10441 attagaaata tcaattatcc cttgctccac atattcatct aatggttcaa tctgattgat
    10501 gtattgagga aattgattgt tcagttggaa aatgtcccag atatcatctg atgctgctag
    10561 tgtacctaat tttgtgtaat atccttcata atcataaaat tcataatcga tcgtcacatg
    10621 gggatttttt tcttcataaa gctcgattac ttttactgtt gcatcgtgtc ggacttgcga
    10681 accccaccaa gccatgttca gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa
    10741 gaagaataac ataacactta aagctaagat gattctcatt cttttcttca ttctctgcac
    10801 ctctacttcc attgttattt ttcaatatac tgtattttta tacagaacat atttattaaa
    10861 cgatcttttt caaggggaat aaaaaccaag acttgcctta tcgttctgcc taggatgaac
    10921 aactcaatac tttcaagaaa aattagatag tgctgattct tctttcatcg ctaaaaacac
    10981 ttcttgcttc aatgatggga ggataattac gcaaagaaaa aaggatatca gacaactgcc
    11041 caatatcctt gacatagcta aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac
    11101 agaaaaataa ctagtaattc tgtatgaaat taggtacaat atacacgtcg atgcacaagc
    11161 attgactatg gcaacttgat tggtcagttc ccgctgactt ttcaagcccc gacaatttag
    11221 ctgctactaa attgtcgggt gcctttttta gtttttattc gtttttcatt ctatagaaag
    11281 cgcttcaata aatcaagaca tttttttgtt agaatgctaa taataacaaa aggttttaac
    11341 tcttcttatg tacattacag gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga
    11401 acaaatagta ctggagtaca aaagacgagg aagagccact atctgctctt cctcgtcttt
    11461 tgattctcac ctgttattca tgattctttt tctcagtttc ccgatatttt tccattttac
    11521 gactaaaaag ctctccgcta cttggtggcg tatatgtgat ggcatttgct cctgcttgaa
    11581 tggtctcagc aatactttct tccgttggac cacctgtcgc aatgatagga agttcaggat
    11641 attttttacg aaaatggcgt actgcttctg cagtttcttt acctgcactg atattgataa
    11701 tcgaaatacc agctgctaac ttttcatcta tttctgtata tttcgacgtt accgtactga
    11761 taactggaat atcaactact cgacaaactt cttttactgt ctctaaaggc gtaggaccgt
    11821 tcaaaacgac ccctaacgaa ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag
    11881 caccttgtgt caaaccacca cctacaccgg agaaaactgg aatgtcagct gcctcaataa
    11941 tgcttttagt aatcgctgga tgaggtgtaa aaggatacac agcaattact gcattcgcat
    12001 ttgtattacg tatgatcgca atatccgtag taaagatgat cgaacggatc aattttccaa
    12061 atatcttgat cccgcttgct tctaaaatga tttccggtac tcttacgata tcttttctca
    12121 attccgtcat gatttctggc gtaaattgtt ctgccatcag ccacttctcc tttgctatct
    12181 ccggtcatac tgattcaatt gatacgtctc gatgacctgg atgatttttt gcgcataacc
    12241 ggggtctgtc gcataacctg catcttgcaa tgcttgtgcg gcttcctgat agttggtggc
    12301 cgtaatcact ttttcataca gcgcaggatt ccaatctact ccttggacga acagttgtgt
    12361 atggtcatcc atggactgtt cccatgaatc atacactttg aagtctcctt ggatagttat
    12421 ccattcttcg ttaacaaatt cctttgtttc caggcttact ttcttctgat ccccataagc
    12481 cttgatcccg aaaagattat tgtatttgct agccaaggta ctttttcccc aatttgattc
    12541 taatatagct tgtcctaaaa tgatactagg aagcactcca taaccatctt gtagttcttg
    12601 ggcatgcgga acaagccgtt caataaattg ctgatgggat tgcgcttcat tttcttgctg
    12661 attatcagta aaaggagaag acagattctt caaggaaaaa acaaaggcta agagtatcac
    12721 tgctacaccg gcaaccactg ctggccaacg gatattcttg cgcttttgct ttttccactt
    12781 ttttttcgcc atgatcaatt cctacttttc atttaattca ttcagatatt cttctagcgt
    12841 caattggtgt ttggtgatat actcagcgtt ttctttaccg acataacgta agtgccaagg
    12901 ttcatatgtg atcttagtta tatcttcgtg tccttttgga taacgaacga taaatccata
    12961 ctgcgcagca ttatctgcga tccatttagc tccaggctga tctccgtaac ttgcatccaa
    13021 tacagtagaa ggataactgt tgtaccagtt ttgatcgacg acatcgactg ccagacctgt
    13081 gtggtgttcg ctgtatcctg gctccgtaat cgtttcttta gtaatctttg tcgcctcctc
    13141 ttctgataca ccttgacgac tcatcacatc ttggacattt tgttcgaaaa cttgttgttg
    13201 ggatgataca gaccggtaag ctgagaccat cactaatgga aatccagctg cttcagcagc
    13261 cttggcaaat gcttcatatt ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa
    13321 tgatgccaat tgagtagact catcaatttc tttttctaat ttgtgatctg gaccaaccaa
    13381 aactagcttc caatcgtctg ttttaacatc tggcaattgt ttggacgtac gatcttttgt
    13441 ttctttgtcc tttaccttgc tgctggttgt ttgactgttc ttttctttac tagcagttgt
    13501 tttggtgctt gttcgttcga ctgcttgatt gccggcttga acatgcgaat catcatcgct
    13561 aaaaataaca tagcccattg ctgcgatcat aatcacaact gctccaaatc ctaaaaattt
    13621 attcacttga aaaatccttt cgttgttctt cacgttcccc attcgcctca tgaacgtaag
    13681 tttcttccag attttcattc acccaatcga gaagttcgat tttttctcct tcgatctgtt
    13741 tttgaacatc tgctggcaga cgaaaattca aaatatcatc atagccccat tcatcatatt
    13801 gaatacttac tttcaagtca aggtatctac cagcctgctg attatttttc aaagggatca
    13861 gctcgacttt tgctgctttg ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct
    13921 tgtactctgc aacggcaacg tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct
    13981 tttgcgttag catccattca taatcagtga aaagattttt tactttctgc atcgcttcag
    14041 atggcagatt tttctcgcca tctttccatt tttgccattg ttctggactg atcagcaagt
    14101 attcatctaa aaaatttttt tcagtttcaa actgttctaa taatacactc ataataagat
    14161 caatatatac ttgatgcacg tttatcccct ccttttaact attctactta agttttgatc
    14221 aaaaagcacc tcttaaatcg ctgctttcat tttttttaat gtttcaagtc ctctcctctt
    14281 tattcttcgt gatttttgtt ataataatag acagacaaga aactcaatcc aactatgaag
    14341 gagcgttttc agatgaaaaa atatcaatgg ggaatcgttg gtcttggtac catcgcacat
    14401 gagtttgccg aaagcttcaa tcaagagacg agcgaactaa cagcagttgc ttccagaact
    14461 tcagaaaaag cggaaaactt cgctcaccgt tacaatattc caaaagctta cggaagctat
    14521 caagaaatgt tagacgatgc tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag
    14581 catatcgacc atatcttggc agcattggaa gcaggcaaac acgttctatg tgaaaaagcc
    14641 atcacgatga ataaaaaaga actggctgac gctatgcgct tagcagaaga aaagaatttg
    14701 atcttagcag aagcgatgac aatcttcaac atgccacttt atcagcagtt acgctcaata
    14761 atggatacag gaaaactggg ggctttaaaa atgatccaag cacctttcgg cagttacaaa
    14821 gaaccagacc ctaaaaaccg gttcttcaat ccagaattag ctggaggagc tctgttggat
    14881 atcggtacgt atgctgtatc tttcgctcgt ttctttttga gctctcaacc tgaagtagtt
    14941 gcctctacaa tggttccttt tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt
    15001 aacaaagaaa atgaacttgc tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc
    15061 ggtatcgttg cctttgagaa cggttatgtg acgattgctg attacccgcg tgctgatcgt
    15121 gcggagatct tatttacaga tggtacaaag gaattcatcg aaagcgggaa tacatctgaa
    15181 gcaatgaatt acgaaataaa gaatatggta cacatgattg aaggtgactt gcccaatcgc
    15241 tctctcttct tgacaaaaga tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa
    15301 aatatgtaat tttaaaaggt tgtgaaaaag ctgttctgca tcaagtaata agaacagcca
    15361 aacaaaaata gctcctccta tttttcgttt ggctggactt attaccgcag aggaaagctt
    15421 ttgaacaccg tttaagaaag aggttgtgag cctaccgttc agctccgagt cacaaggagc
    15481 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttga
    15541 caggagaaca ccgtttaaag aagaggttgt gaaatcagct ttttgacctg aatattagct
    15601 gaattcacaa cctctatttt tggatttttt gttgggagaa ttctgccttg atttcaataa
    15661 aacttttaaa agactgcaaa aactggaaca attctgctcg atcctcgaat tcagggcgac
    15721 tagtcggcag aggcatttgc cgatacaata cataaacttc ttcaatacgt gttaaaattt
    15781 gtctgccatc atttgactct gaaaatgttt ctgcagtata aatcaataaa ccatagatat
    15841 gttttccata ataagctggt tgctggattc tctctagatt ttccagcatg tctttaatca
    15901 cattggcctg tgctcttctc atagagaagt aagtttcgta gtatagcggc tgtctgagcc
    15961 agtagttttc ttgatgttct ctggcatctt cttggctttc gcgaatgaat gtcagtaaat
    16021 gttcgcattt gatctgcaca tcttctttat tttctgataa aagaaattct gccatttgtc
    16081 tcaaaatatt tcgaaattct ttttcgatct gtatttggtt ttccttcagc tgcttcgtac
    16141 gatctggcat gtaaacattt gcaagcaaag caaatactaa tccaatcgcc attaatcccg
    16201 cttcattgcc gatcagctgc caagacatgt tttcctccac taaataatga gtgaccaaaa
    16261 ccgaatttac tactatccct tcggttaatt tgaagcgaga ggcaagcggg atgaacagga
    16321 ataaaaaaag actgaagcca aaaatagtat acccaacagc aaaaaaactg aacagtgctg
    16381 ttgccgtagc taatataaag gctgcgattc gattcatgcc cgtacgcaat gtagagcgct
    16441 tggtatttcc tacacttaat aatgcaatga tccctgcagc tggccaatac aacagcgata
    16501 agctacccgc tgtgaccatc gaaaagaaga cactgatcac tgttttgact gttcgcattc
    16561 ctatacgcat ttacttcccc cttttttatt ttaattatac ttgaataatg aaaaactagc
    16621 tatcagacaa aagtttaggt atgatagtaa aaagaccaaa aaggagaatc tctatgcaat
    16681 ttggccgttt tcgactaggt atgcgtacga tcaaaagtgc cttagctgtt tttttatgta
    16741 tcttgttttt tcatttgact caacgaggaa tcccgatgat tgctgcatta tctgctgttt
    16801 tttctttgcg tcaggacttg actacaaccg tttcttttgg aaagtctagg atcatcggca
    16861 atacattagg tggtacatta gctatcattt atttttttgt caaaagctat tttcacaatg
    16921 attttttggt agaattgatc ttgcttccta ttctagttat tattgtgatc gtcgtgtcag
    16981 atggtattaa caataattcc ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt
    17041 tgagcatccc tcaaggcgaa tccgctttgt atgctatcca acgtgtacta gatactttta
    17101 tagggacatt cattgcaatc ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga
    17161 aggaagaact atcagaagat ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa
    17221 atttacaaga gatagaagct aagatccaaa aacaaaagaa aaatagcgag gactaatttt
    17281 cttttgcaat aaaataaaaa aatggagaga agaactttaa aaagtcttcc tccatttttg
    17341 attgaacggt gaagtgtact actttattct tccttcatcg agttttgttc tgcctctttt
    17401 ggcgcttcat acatatttac ttggctagta gtcccacgtg tcgacaaatt ctctacgatt
    17461 tccttcaagt caagtcctgt tgtcgctttt agtgtttctt gagtagaaga aagcaaattt
    17521 gtggcataat ttgtcactcg gttcgctcca ctggtttctc ctgaactgct tcctgtatcg
    17581 acaacagaga ttttttcgat atttcctagc ggctgtgctg cttcgcgcat caattgcggt
    17641 agcatatcga taaccatact caagacagct gcttcgccat attccttgaa ggcattggcg
    17701 atcttttgtt ttgcttcggc ttctgctttt ccttttgcta gtgctgcttc ggcttctgct
    17761 tgtccagcta aacgtgtctt attggcttcc gcttctgcca gtgcttccac acggaatcgt
    17821 tctgcttcgg cttcagtcac ttctttgact ttttgagctt gtgcttcttg ttctttagcg
    17881 taccgatcgg catctgcttt tttcttcact tccgaatcat attgcttttc acgacgtgtg
    17941 atttcttttt cttccagttc gatttgtttt tgacgttcta cgattttaat ctgcatttcc
    18001 tgttcgatta cttgttgttg tgctcgggca ctttctaaat tgtaggcttg gtctgctttt
    18061 gctttagcaa catcttgttc ttgtttgtag gttgctaatt tcagttcttt ttcttttaat
    18121 gattctgcga tctctgtttg tcgctgtaat tcagctgctt gtgattcttt ttctgcttga
    18181 gcttttttga tacgcgtttc ttttagagct tccgcttctg ctatatctgc atctcttttg
    18241 acttgggcga ttcttggttt ccctaaagag tcaagataac cgtttttgtc tctgacctct
    18301 ttgatagtga aagaaacgat gatcaacccc atttttgcaa ggtctacaga agcgacttct
    18361 tgtacgcttt gactgaactt atcgcgattt tgatagattt cttctacagt catagaacca
    18421 agaatcgagc gcaagtgtcc ttctaatact tctcttgctt cattttccag ttcttctctt
    18481 gtcttgccta aaaactgttc tgctgctgta gcaatctctt ctacagatga accgattttg
    18541 atgatcgaag taccatcaca catcaccggt acgccttgtt ccgtatacac ttcaggggtg
    18601 gagacgtcca atttactcga taatagactg atccgattcg aacgttgaaa aactggcaag
    18661 acgaatgcgc cacctccacg cacaattttg attttgttat tgctttcatc aatgtgcaca
    18721 tttttgcttc ctaaataact tccactgatg atcaacgctt catccggttt tgctgtctgg
    18781 tacttcgcta caaagacgat cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg
    18841 ataaaaacaa ctggtatttc catttaacac acgctccttt aagcttttat catgctttca
    18901 taaggcatga cataacagat tctttccttg atctcaatga tcaatacttt cgttccccgc
    18961 tcagctgtct gttcttgggg gctgtaaaaa caagctggac gactaatgga accggtaaca
    19021 cttttcaact gaatttctcc catacctcgt acagggatcg gtgtgatcac aacagctact
    19081 tgtccttcca gagttttttc agaagacgac atcgttgatt cggcatgttt catcggcatc
    19141 aatatataga aattaagcaa aaataccaac actatcgata tgcctccact agcaataaat
    19201 aaaaataaag atgattgatc tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa
    19261 gttaaccaag ggataataag catcggatca agcgggccat caaaatcgaa tacatctcca
    19321 aaaataatca ataggactgc caatcctgca caaatcagca atgtatacca ataaatcgtc
    19381 tcaagtgcga ttccccctat catatacacg cctccttcca tttctacgtc tatcatacca
    19441 atgtttgaaa attctgtata gcttctttta ccgactcagt ggtgtttttt ataggaaggt
    19501 tgtgtttatc gttgacaatt tgttaaattt ttgttttcat gttaaaattt tcacaataaa
    19561 ttctttcgag gtagcctatg tttccttttt ttaagaaaaa accattaaag ctccagaaca
    19621 cgatcatcct tgtcgtttgt ttctccacat tcgtttcttt gattgtttct gccatattga
    19681 tcaggaattt tattctttcg atgaaccgat ctagtgtgtc agataaaatc tctagcattg
    19741 ctaaaatcgt tgccaatgat aaactcatcc aaaaaaagct gcagactggt gacccgacac
    19801 atgcgattca aggaataaca aatgaaatca tgattgcatc gaatttagat tttatcgttg
    19861 tcatggaccc tgaaggcatt cgatattctc acgcagaccc aagttacatc ggtcttcctt
    19921 tctcaagtca tgaagatgtg aaaaaagtga ttggaggaca agaatttttc tctgaaaaag
    19981 aaggaatgtt agggttcggc ttccgctatt tcgtccctat ttattatgat caagagatta
    20041 ttggtgcagt ttgtgttggc ttaacaaaca acacgattga caatcaagtc aaagatggac
    20101 aaaaaatgat tgttttttca ttgattattg gacttagcgt aggcatcata ggcgccgttt
    20161 ttcttgccca aaaaatgaaa aaagtactgc ttggacttga accacaagaa atagcagaac
    20221 agttaagtga aaaaacgatt atcgaaaatg aagtagatga aggaatctta gccatctctt
    20281 ctgataaaac tatcctcttg atcaatacac ctgcaaaaaa cattttctac caaacagagc
    20341 agctattgga tttcgaagta ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa
    20401 agagacgatt gagcatcaaa cgaccgttcg gactcaatcc atgtatttaa atgggataga
    20461 aactatttct agcgttacac ctctttttag caatgaccgt tttttaggag cagtcgcaac
    20521 tttacgaaat cgatcagaag taatgaaatt aatggaggag cttgggggga cgaaacaata
    20581 tataaactcc atgagagctc aaacccatga attcatgaat aaacttcatg tcatttctgg
    20641 tttgattgat ttgaagaagt atgaagaagt cacccatttt atccagcagc taaatcaaaa
    20701 ttataaaaaa gagattggcc aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt
    20761 tttattaggc aaaataaacg aagccaaaga acaatctgtt gaaatgctgt tagaaagcga
    20821 ttcttttatc cctaaaatgg agatggatga ctccatccat tcacttttgc aagtgttagg
    20881 gaacttactt gataatgcaa aagaagccgt gctacaaaaa catgaaagaa acggacaggt
    20941 ccggattcat ttaaactatg acacagaagc tgaagtattc atcgtgaccg tctgcgatga
    21001 cggtgtcggg atatcagaag aaatctcaga aaaaatattt acgctaggtt tttcgacgaa
    21061 gggaatcaat cgtgggtttg gattgaatct catccattca atcgtcaaac gccacggtgg
    21121 atttttagaa ttttccaaac aaacaaatgg cggtacaatc gtctatctag aattaccaaa
    21181 aaaggaagat caaaatgatg acaaatgtcc tgataatcga agatgatcca atggttgcag
    21241 aattgaatcg tacttttgtg gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa
    21301 atgtagaaga aggaaaaaaa tatttcagac aaaaaaacgc agatctgctg ctgcttgatg
    21361 tatacatgcc tggcgaaagt ggtatccatt ttttatcttg gcttagacag caaggaaatg
    21421 atatcccagt gattttgatc actgctgcag atgatatcga aaccataaag aatgtgatcc
    21481 gctacggtgc cttagattat ttgatcaaac cgttcacttt gcaacgtttt cagctggcga
    21541 tcgaaaagta tcttgctttc tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat
    21601 tgatcgatca agtattcaac cacaccactc ccattcacga aataccatca ttcatcgatt
    21661 tgcctaaagg tctgtcaaaa ctgactttaa aaaaaataag tgcatttatt ctaaaagaag
    21721 atactatttt ttcaacaaaa gaactggctc aaaaaagctc actgtcacgt atatccgtca
    21781 agaaatattt gaatttttta gtagaacaag atatattgaa cgaaagaatc aaatatttgg
    21841 aaatcggccg gccgctaacc tgttatgaat tgacaaagaa tgggcggaaa aagctcacag
    21901 attactgttg agtcatcgaa ttatcccata caaaatagtc caagtataca gaagtaacaa
    21961 ctgtttactt ggactatttt taaacatttt attgacgaat atccatgttt tcttatctta
    22021 attgacgggc tttaggaaga gatacctcat ttttcgtttt tccagtttct attatttcac
    22081 gaaagttctc gaaacttgtc gaaatcatat ttgataatgc ctcatctgta tttgaaccta
    22141 catgcggggt tacaagaact ctagggtaca tttctactaa ttgctggata gcagggtctg
    22201 gaatcgtctc ccatggcttg aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat
    22261 cggttgcaaa accagctaaa tgattgcttt tcaaagctct taaaatagct tcattatctt
    22321 gaagctctcc acgggctgta ttaatcaaga tgctaccttt tttcatttga gctaaaaatc
    22381 cttcattgat catttgatcg ttttcacctg ggatatgagg tacatgaata ctgacaatat
    22441 cgcttttggc taataactct tcaagcgagt ggaacgtcaa tactttttta gcagcttctg
    22501 tctgatgaat atcgtagccg atcactttgg ctcctagtcc cttaaacaaa acagcttccg
    22561 tcaaccctat cctgcctgtt cctatgatcc ctactgtgca atttctgatt tcacgactaa
    22621 acatttgctc atccactcga aaatctttga aagatgttcg gatcgtcgta tacgcagtat
    22681 ttctcaaaag catcatggca aaagtcaaag ataactctgc tatcgcattt ggcgaatacg
    22741 atggtacacg ggctactgtc ataccaaaat cagctgccgc ttgtagatcg atatgattga
    22801 ctcccacagt ccgtgtaaaa acataacgaa tcccatattc ctgcattttt gcaagattgg
    22861 tctcatctgc tatacaattt cccctcaaca atacagcatc atggtccttt gctgacacga
    22921 tattgtcatg tgtcaacagt tcttcataaa gactgagatc ataatggtaa atattcaatt
    22981 tttcaaaaaa atctatctcg ttcggacgaa caccgtaaca tgcgattctc atactttctt
    23041 cctccatttc ttatgaaaat aaacctatgc catgaatcat ttgtaataca acgatccata
    23101 caggtgtcag taccacagca aaaatagttg acagtaagga tgcattggag gctaaaagtg
    23161 cctccctatc aaaactgata gcataggctg ctgccactgt tgcagctggt gtagccatca
    23221 tgataacgat tgttgccagt gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa
    23281 ctgctagaat cacgatattc aatgcaggta caaataccac tttgactaga ctgtaatacc
    23341 atgaagtttt ttcagatgca gcttttttga agcttacttc acctaatgtt gcgccgattg
    23401 ctagccacgc caatggtgaa cttaaaccag ctaaataatc catcggctga aatagccacg
    23461 gtgcagtctg gtcaattcgc aaaaacgcta cttggcttat ctctccttct gcacctgtaa
    23521 ctgacacttg tggcagatac ccttggaaca gccaaatgaa aaggccggca aaagtagcga
    23581 taatgattgg attgacgaac atcgctttga tatttttagc ttccatcttc aagccactca
    23641 tttttatgta tccataagaa tacaaaaaga ttcggtagcc aatattgaaa attgaggaat
    23701 acatcacgcc gacaggacca taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag
    23761 aaccaaagat cgtcaggaca cgcaacgttg tttgcttatc ccctttatag cttaagaaaa
    23821 aaggttttga aactagaata agaataatat aaataactaa tccccaaatc aagacattga
    23881 ttccttgcat cagtaattct gtattgatat cttgcatgaa agctcgaaat gccagtgctg
    23941 gcaaagccac tgttaagacg actttcgata gaatttttcc aacagattca gaaaaaatcc
    24001 ccttttttct acaaacaaac cctaataata taataaaaac tgttgaagtg attgcactga
    24061 caatcgacat atctgtcaaa gtcgatcgaa aaatgtcaaa taagttcatc tttctcctcc
    24121 taattatgtt catttattca caataaaaaa agcttttatt ttgctaatac gtattaacat
    24181 ttttaatttt atctttacat tgttacttat tgaacaatag gaaattaaag aaacttttga
    24241 aacttatgta aagtgatt
//
LOCUS       DAZKOR010000040        23829 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000040 DAZKOR010000000
VERSION     DAZKOR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.040"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACG8ZM_001488"
     CDS             complement(53..787)
                     /locus_tag="ACG8ZM_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0492453.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACG8ZM_001489"
     CDS             1114..1524
                     /locus_tag="ACG8ZM_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0492454.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACG8ZM_001490"
     CDS             1517..2218
                     /locus_tag="ACG8ZM_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0492455.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACG8ZM_001491"
     CDS             2218..3804
                     /locus_tag="ACG8ZM_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0492456.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACG8ZM_001492"
     CDS             3823..4044
                     /locus_tag="ACG8ZM_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0492457.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACG8ZM_001493"
     CDS             4041..4802
                     /locus_tag="ACG8ZM_001493"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0492458.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACG8ZM_001494"
     CDS             5182..5691
                     /locus_tag="ACG8ZM_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0492459.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACG8ZM_001495"
     CDS             complement(5766..6305)
                     /locus_tag="ACG8ZM_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0492460.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG8ZM_001496"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG8ZM_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0492461.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACG8ZM_001497"
     CDS             7318..8169
                     /locus_tag="ACG8ZM_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0492462.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACG8ZM_001498"
     CDS             8197..8832
                     /locus_tag="ACG8ZM_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0492463.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACG8ZM_001499"
     CDS             8852..9625
                     /locus_tag="ACG8ZM_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0492464.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACG8ZM_001500"
     CDS             10033..10830
                     /locus_tag="ACG8ZM_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0492465.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACG8ZM_001501"
     CDS             10808..11221
                     /locus_tag="ACG8ZM_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0492466.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACG8ZM_001502"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACG8ZM_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0492467.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACG8ZM_001503"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACG8ZM_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0492468.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACG8ZM_001504"
     CDS             15998..16558
                     /locus_tag="ACG8ZM_001504"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0492469.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG8ZM_001505"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG8ZM_001505"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0492470.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACG8ZM_001506"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACG8ZM_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0492471.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACG8ZM_001507"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACG8ZM_001507"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0492472.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG8ZM_001508"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG8ZM_001508"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0492473.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG8ZM_001509"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG8ZM_001509"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0492474.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACG8ZM_001510"
     CDS             21905..22483
                     /locus_tag="ACG8ZM_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0492475.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACG8ZM_001511"
     CDS             22535..23017
                     /locus_tag="ACG8ZM_001511"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0492476.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACG8ZM_001512"
     CDS             23134..23508
                     /locus_tag="ACG8ZM_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492477.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4286 c         5114 g         6647 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtagactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtttt
//
LOCUS       DAZKOR010000041        23653 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000041 DAZKOR010000000
VERSION     DAZKOR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.041"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..506)
                     /locus_tag="ACG8ZM_001513"
     CDS             complement(<1..506)
                     /locus_tag="ACG8ZM_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFQ0492478.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            779..3250
                     /locus_tag="ACG8ZM_001514"
     CDS             779..3250
                     /locus_tag="ACG8ZM_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0492479.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3353..5089)
                     /locus_tag="ACG8ZM_001515"
     CDS             complement(3353..5089)
                     /locus_tag="ACG8ZM_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0492480.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5086..5790)
                     /locus_tag="ACG8ZM_001516"
     CDS             complement(5086..5790)
                     /locus_tag="ACG8ZM_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0492481.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5921..6424
                     /locus_tag="ACG8ZM_001517"
     CDS             5921..6424
                     /locus_tag="ACG8ZM_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0492482.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6384..6722
                     /locus_tag="ACG8ZM_001518"
     CDS             6384..6722
                     /locus_tag="ACG8ZM_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0492483.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6743..8161
                     /gene="ffh"
                     /locus_tag="ACG8ZM_001519"
     CDS             6743..8161
                     /gene="ffh"
                     /locus_tag="ACG8ZM_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0492484.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8273..8851
                     /locus_tag="ACG8ZM_001520"
     CDS             8273..8851
                     /locus_tag="ACG8ZM_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0492485.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8855..9376
                     /locus_tag="ACG8ZM_001521"
     CDS             8855..9376
                     /locus_tag="ACG8ZM_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0492486.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9455..10009)
                     /locus_tag="ACG8ZM_001522"
     CDS             complement(9455..10009)
                     /locus_tag="ACG8ZM_001522"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0492487.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10262..10537
                     /gene="rpsP"
                     /locus_tag="ACG8ZM_001523"
     CDS             10262..10537
                     /gene="rpsP"
                     /locus_tag="ACG8ZM_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0492488.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10549..10800
                     /locus_tag="ACG8ZM_001524"
     CDS             10549..10800
                     /locus_tag="ACG8ZM_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0492489.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10925..11716
                     /locus_tag="ACG8ZM_001525"
     CDS             10925..11716
                     /locus_tag="ACG8ZM_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492490.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11886..12407
                     /gene="rimM"
                     /locus_tag="ACG8ZM_001526"
     CDS             11886..12407
                     /gene="rimM"
                     /locus_tag="ACG8ZM_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0492491.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12397..13158
                     /gene="trmD"
                     /locus_tag="ACG8ZM_001527"
     CDS             12397..13158
                     /gene="trmD"
                     /locus_tag="ACG8ZM_001527"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0492492.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13294..13641
                     /gene="rplS"
                     /locus_tag="ACG8ZM_001528"
     CDS             13294..13641
                     /gene="rplS"
                     /locus_tag="ACG8ZM_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0492493.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14014..14676
                     /locus_tag="ACG8ZM_001529"
     CDS             14014..14676
                     /locus_tag="ACG8ZM_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492494.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14755..17982
                     /gene="fss3"
                     /locus_tag="ACG8ZM_001530"
     CDS             14755..17982
                     /gene="fss3"
                     /locus_tag="ACG8ZM_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0492495.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18096..18410
                     /locus_tag="ACG8ZM_001531"
     CDS             18096..18410
                     /locus_tag="ACG8ZM_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0492496.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18423..18797
                     /locus_tag="ACG8ZM_001532"
     CDS             18423..18797
                     /locus_tag="ACG8ZM_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0492497.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18807..19151
                     /locus_tag="ACG8ZM_001533"
     CDS             18807..19151
                     /locus_tag="ACG8ZM_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0492498.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19222..20574
                     /locus_tag="ACG8ZM_001534"
     CDS             19222..20574
                     /locus_tag="ACG8ZM_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0492499.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20634..21776
                     /locus_tag="ACG8ZM_001535"
     CDS             20634..21776
                     /locus_tag="ACG8ZM_001535"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0492500.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21801..22055
                     /locus_tag="ACG8ZM_001536"
     CDS             21801..22055
                     /locus_tag="ACG8ZM_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492501.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            22311..23495
                     /gene="mobT"
                     /locus_tag="ACG8ZM_001537"
     CDS             22311..23495
                     /gene="mobT"
                     /locus_tag="ACG8ZM_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0492502.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            23492..23629
                     /locus_tag="ACG8ZM_001538"
     CDS             23492..23629
                     /locus_tag="ACG8ZM_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0492503.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7834 a         4218 c         5071 g         6530 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      661 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      721 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      781 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      841 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
      901 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
      961 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1021 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1081 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1141 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1201 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1261 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1321 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1381 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1441 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1501 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1561 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1621 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1681 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1741 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1801 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1861 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     1921 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     1981 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2041 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2101 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2161 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2221 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2281 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2341 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2401 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2461 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2521 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2581 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2641 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2701 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2761 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2821 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     2881 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     2941 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3001 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3061 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3121 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3181 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3241 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3301 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3361 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3421 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3481 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3541 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3601 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3661 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3721 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3781 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3841 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     3901 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     3961 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4021 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4081 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4141 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4201 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4261 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4321 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4381 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4441 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4501 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4561 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4621 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4681 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4741 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4801 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4861 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     4921 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     4981 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5041 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5101 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5161 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5221 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5281 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5341 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5401 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5461 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5521 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5581 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5641 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5701 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5761 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5821 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     5881 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     5941 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6001 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6061 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6121 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6181 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6241 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6301 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6361 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6421 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6481 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6541 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6601 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6661 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6721 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6781 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6841 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     6901 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     6961 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7021 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7081 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7141 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7201 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7261 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7321 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7381 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7441 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7501 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7561 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7621 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7681 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7741 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7801 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7861 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     7921 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     7981 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8041 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8101 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8161 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8221 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8281 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8341 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8401 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8461 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8521 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8581 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8641 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8701 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8761 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8821 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     8881 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     8941 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9001 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9061 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9121 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9181 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9241 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9301 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9361 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9421 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9481 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9541 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9601 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9661 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9721 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9781 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9841 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
     9901 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
     9961 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10021 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10081 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10141 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10201 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10261 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10321 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10381 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10441 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10501 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10561 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10621 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10681 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10741 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10801 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10861 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    10921 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    10981 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11041 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11101 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11161 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11221 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11281 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11341 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11401 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11461 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11521 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11581 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11641 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11701 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11761 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11821 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    11881 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    11941 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12001 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12061 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12121 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12181 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12241 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12301 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12361 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12421 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12481 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12541 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12601 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12661 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12721 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12781 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12841 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    12901 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    12961 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13021 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13081 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13141 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13201 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13261 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13321 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13381 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13441 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13501 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13561 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13621 atcaaagaaa tcagaagata aacacggaaa acttccttgg aaatctgaaa tttgtgaaaa
    13681 gtccttgtat atcaagggct ttttcttttt tctctatcac agttttaagc catttttggc
    13741 tgttttggac catttttcaa tcatattccc ttaaaaattg tcctagattg tccgacattg
    13801 actatcagtg tcgtcaaaat agggcaaaat ttagtagtca gcttgacgac aaatataata
    13861 cgaaaaatac caaaacattt accgaattta gcaacttttg aacccaagtt tctcaaaggg
    13921 tcagctatta gcaaaaaaga caaaacatgt acttttctac taaaaagttg atgtgatagg
    13981 cttctatcta aataactatt aggagttttt atcgtgaagc aaaatgatgg acgtatcatt
    14041 tggaaaaatc aaagtgagtt aaaactgatt ttaacaatta atgaattcat cgaaaaacac
    14101 ggaattacat ctagccgtca ataccagaaa aagctatcag aaaatccaaa ttctgcacca
    14161 agtatgtggt tcattaacaa aaaatatgga tcatgggaga atttattgat tagtatagga
    14221 cgagaaaata ctgggtatgg aaaatgggct agaatgtcag aacaagaatt attggaaata
    14281 gtagaagcct ttattaagtg cgagaagata acctctcaac gaatgtacga acaaaaatca
    14341 gtagggaaga atattccttc acttagtacc attaaaaaga tgttaggaga tatacgtcca
    14401 ctttttaaag aaaaaaatga tggatctcga tttactgatt tcgaactgct gttagaacta
    14461 aaaaatgaaa taatccgatt aaaactacag gatgatttat caatgacaaa gtttcgaaaa
    14521 ctagttcaat ccccaaaact accatcagtt gatacaatta tgaaaagaac gaacaaaaat
    14581 tgggaggaat tgatggcaga gattggattt gattatcgaa gaatcaaaat ttataagcag
    14641 agaaacaacc tatccaagac aaagaaaact aaatagtatc actctaaatc agacgttcaa
    14701 aattaaaatt tggacgtctt ttttattacc caaaaacgaa aggagattta ttttatgaaa
    14761 aagccaagat ttaagaattg gcgtttgctc gcaacactga gtctattatg ccaaacaatt
    14821 ggtggatcgc ttggcccaac gattgccttt gctgatgaaa ttactcatcc acaaacggta
    14881 actgtggaat tggatttggc tcaccagtat gctgtcgaag gtacatttag cgatggtcgt
    14941 cccatgtcag aagttaccgt tccgcactat gcagtttaca atggtgtgaa gcaagatatt
    15001 ttctgtattg agccaggggt gccgatttac aatgagttca ctcctggtta tgagaaaaat
    15061 ccattgcctg atatgtcgga gaaagcgaaa ttagtttccg ttttatggaa ggaagctggt
    15121 acggatgtgg atacacatat tgtggcccag aagatgattt ggcaagaagt taatggctac
    15181 acgcttcact caattaaacg ttctgatggt agtgcagtaa atatcgcagc cattgagtca
    15241 aaaatcaatc aagccattgc ggattatcaa aagaaaccaa gcttccataa ctccacagtg
    15301 aaaaccgtgt taggtcaatc gactactgtg acagatacga acggcttgaa tttatcggag
    15361 tttgatgaag ttgtcgagaa tatcgcaaat attgattatc gtgtgaacgg caatcaacta
    15421 gtgattactc caaatgccaa ttcaaaagag agtggtgaat taaccctaaa gaagtcagct
    15481 aatacaggaa caccggtagc ttataaaatg gctggtcaac aaactttgat ggcgggggca
    15541 attgataagc ccaataccta cacaatcaaa attgatgtgg aaactgaggg ttctttgaag
    15601 attaaaaaag tcgataaaga aacaggaaat gttgtaccgg gaaccgtttt ccatttagac
    15661 tttggaaaaa cattacctgc aaaagacgtg accaccgata aagaaggcat tgcgacctta
    15721 gatgagattc ctcatggcgc gaaagtaacc attactgaaa aatcagtgcc agctccttat
    15781 acgattgaca ctacaccaat caccgcaacc attaaatctg gtgagactat ttctgtgact
    15841 tcaaaaaata ctcgcgaaaa aggtcaaatc attttgaata aaatgggggt ggaaacagga
    15901 actgatcttt ggaacgacaa atactcatta gccggaaata cgtttgccat tcgcaaagac
    15961 agtccaaccg gtgaaattgt ccaagaaatg accacggatg aaaaaggtca tgcggaaaca
    16021 ccaaaagaga ttgccaatgc gttggaattg ggaacctatt atgtgaccga aaccaaagct
    16081 agtaatggct ttgtgaatac ctttaaacca gtaaaagtcg aattgaacta tgtcaatcaa
    16141 accgtggctc ttgttaccag taacgtaaaa gggcaaaacc aagaaatcac tggtgaaacc
    16201 actttgacaa aagaagacaa agataccggt aatgaaactc aaggtaaagc tgagtttaaa
    16261 ggagctgaat atactctctt tactgcaaaa gatggtcaag cggtcaaatg gagtgaggct
    16321 tttaaaccag agttagtgaa gggaacgaaa gcttctgatg aaacggtgac tttgacttta
    16381 gatgaaaaga atcaagttgc tgttaaacac ttagcgatta atgagtatta ctggcaagaa
    16441 accaaagcac ctgaaggata taccttggat gaaacaaagt accctgtttc catcaaaaag
    16501 gtggatgata acgaaaaaaa tgccgtgatt acccgagatg ttacggcaaa agaacaagtc
    16561 attcgctttg gctttgattt ctttaaattt gctggatcag ctgctgggac tgccgaaact
    16621 ggcttcaatg atctgacctt taaagtatcg ccattggaag gaaccagtga gattacaggt
    16681 gccgaagatg aagcaattac agcttataac gagcaactag gttttgatgg ctatggcaag
    16741 tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaggctcc agaaggattt
    16801 caaaaaatta cgccattaga aatccgttct acttttaaag aaaataaagc ggattacact
    16861 aagagtgaat acatttttac gattaccgaa aaaggacaaa aacaaccaat taaaacggtg
    16921 accgttcctt acgagaaact gacgaataaa gcattttctg ttagcttgaa tcgtttgatg
    16981 ctctatgatt tacctgaaga agaagatagc ttaacttctc ttgggacttg gaaagacgga
    17041 ggcaaagaat tgacagacct tgattctacc gagctagttg ataaattgag ctataacttg
    17101 catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga agcgacaaaa
    17161 gctgcccaag aaaaagatga aaaagccaaa ccagtggtaa ttgccgaaac aaccgcaacg
    17221 ttggcgaata aagagaaaac aggaacttgg aaaattttgc ataaattaac cgcagaacaa
    17281 gttttggata aaaccatcgt cttattcaac tatgtgtatg aaaacaagaa accctatgaa
    17341 gcaggtgatg atccagtagc gaaagatgct agcttgaaca atcaagccca aactgtcaat
    17401 tgtacggtgg aacgccatgt ttccatccaa acaaaagccc acctagaaga tggttcgcaa
    17461 acctttactc atggggatgt ggtggatatg ttcgatgatg tgtcgattac ccatgatgta
    17521 ctggatggct caaaagaagc ttttgaaaca attctgtatg cattacttcc ggataatacg
    17581 accaaagaaa tttggaaatc tggcaaaatt gattatgaag tgaatgacaa agaattcact
    17641 aaaaccgtac ttgcggaaaa agtggacaca agtaaatatc cagaaggtac aagttttacc
    17701 ttcaaagaaa ttaactatgg caaagacgga aacatcaatg ggaaacacaa tgaggatcta
    17761 aaagaaaaaa ctcaaacttt aacacctaaa aaagttccga caacaccaag tgcaccagaa
    17821 caaccggaaa taccaactgt tccaagtgac tctcaagaat ctagtcctac agtaaagaca
    17881 ttcccacaaa caggggagaa aaattctaat gttctgctgt ttattgggtt taccttgatc
    17941 tttgcgactg ctgggtatta tttctggaat cgccgaaact aaggtgatgt gatgaaacaa
    18001 ctaaatagga aaaaccaacg tgcgcgaccg cctcctaaaa aaagtggtcg cgctattttt
    18061 gtatccaaaa ctaaaaatga aaagaggaaa agaagatgga attgaaattt gttgtgccag
    18121 atatggccga aacctttgga aagataaggt atgccggcga aggcgaagtt ttaactgaag
    18181 gatatgggcg gaatactaca gtaattggtc gtagttatca tttgtattcc agtaagcaac
    18241 gagccgatga tattgaagtg gtggtagctg cggaagccgg cgaaaaggat tttgatcaag
    18301 atcaaccact aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
    18361 gtgggtttac cgactacgtg ttgtatgtcg atgatttagt gaaagcatag gaggaaaaga
    18421 agatgagatt aacagaaggt attgttgtag attcaggact aacgtttggg aaattacgat
    18481 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacggtt agtaacgagg
    18541 taaaagaacg gacttataat ttgaaatcct ctgcgcaagg tcgaatgatc caagttagta
    18601 tcccagcaaa tgtaccctta cgcgagtttg cctatgacgc agaggtagag ttggtcaatc
    18661 caatcatgga tacggtcgcc aattatgttt tccgagaagg aactactgtc aattggttca
    18721 ttaaggccga tgatttagtc ttgaaacgac aaccagatca gggaaattct acaaaccaaa
    18781 atgaaggaaa gaaataggtg aagaatatgg aaattaatgt tgaacatatt ttagattgtc
    18841 tcgatcaata tggaaaaggt gagctaacgg aggaacaact cactaaagca ttaacctatg
    18901 atgaaaaaat gtttctgatt atgcatcaag ggttactgga agtaggtaac gatgtcgaag
    18961 aagattttga tgtactgaat tggttagggg aagaagagtc cttctttatg gttatcgaag
    19021 taaatgaaag tctatgtcga gaagccgaaa ccgtattgga agaaattggt gtggaaatgc
    19081 cggatgccat tgaagttttt ttaaagcaac tagtggaaac gaaacaactc cctatagcag
    19141 taaatgacta gcataactgc ttgaaattgg atcaatgaaa tcctatgaag cttttttcga
    19201 ttggaggtgg aaaccataaa aatgtacaaa gggcatcgta tacgggcagg agatcagcac
    19261 ttggtttatc actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc
    19321 ttttattttc aagagcttag acaatttgat atttctaagc tatcactttc tactatagaa
    19381 atcgttcgtt ctataaaaga tttaatctat ttgctaggaa gtctcgttct ttcaggaagc
    19441 acgatgttgc tttatataca cttttttcag gatcattggc gcagtttgtg gcatcgacaa
    19501 aagctagccc ggatgattct agaaaatcat tggtacgaag taaaacaaac tcagagtgaa
    19561 ggatttttca aagacctcaa tagtagtcga accaaagaga caatcagtta cttccccaaa
    19621 atctattacc gtatgaagga tggtttactt tctattcgcg ttcaaatttc actgggaaaa
    19681 tatcaagatc agcttttaaa gctggaaaag aagttagaaa gtggtctata ttgtgaacta
    19741 gtagaaaagg aactcaaaga ctcctatgtg gagtacactt tactgtatga tataattgcc
    19801 aatcgaattg ggatagacga agtagtggca gaaagtgggg ctttgcgatt gatgaaaaat
    19861 caagtatggg cttatgattc tttacctcat atgttaatcg ctggcggcac aggtggtggg
    19921 aagacttact ttttactcac tatcatcgag gctttattaa aatctgatgc cgagcttttt
    19981 gttcttgatc ccaaaaatgc ggacttagcc gatttaggta cggtaatgcc tcatgtctat
    20041 tcccaaaagg aggaaatttc tgcttgtgtg gaagatttct atgagcgcat gatgactcgt
    20101 agcaaggcaa tgaaagaaat gcccaactac aaaacaggag agaattatgc gtttcttgga
    20161 cttccaccaa actttttaat ctttgatgaa tacgtggttt atatggaaat gttaacgacg
    20221 aaggaaagtg cggtgatttt gaataagctc aaacaaatcg tgatgttagg tcgtcaatct
    20281 ggtttctttc tgattctagc ttgtcaaaga ccggatgcga aatatttagg agacgggatt
    20341 cgcgatcaat ttaatttccg ggtggctttg ggtcgtatga gtgaactggg atattccatg
    20401 atgtttggcg aggttgataa aaaattcttt atgaagcgta tcaaaggtcg aggctatgta
    20461 gatacgggag ggagtgtgat tagtgagttt tatacgccac ttgtaccaaa aggatatgat
    20521 ttcttaaggg aaattagcga cattggtgga ttaaacgtta atacggaagg ataaaataat
    20581 agtatgtagc ctaggagtgt caagaatcct gggctttttt tacgaggtga tttatgacat
    20641 ttttagattt attcgctggg attggtggct tttgcttagg tatggaacaa gcgggtcacc
    20701 agtgtatcgg cttttgtgaa attgatgatt ttgctcgcca gagttataag gcaatccatg
    20761 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagacgag tttattcaat
    20821 ctctcggacc agtggatatc ctttgcggtg gatttccgtg ccaagctttt tcaattgcgg
    20881 gaaagcggca aggattttcc gatactcgag gtactttatt ctttgaaatc gctcggttcg
    20941 ccgctattct ccaacctaag tttttattcc ttgagaacgt ccggggatta ctcaatcacg
    21001 aaggaggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgatgtgg
    21061 aatggcaagt gcttaactca aaggcctacg ttccccaaaa ccgtgagcgg atcttcctta
    21121 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga tcgtctccaa
    21181 catctgatca aaacatcaga aacttattaa atatcaaccc ttccaatcga ggaatggggg
    21241 gacaagtcta tgggtcagat gacgtagcgc ctaccttgac cggtgatgaa ggaatcaaaa
    21301 tcgcattgcc ggtagatgat ggaatttctg tagcgggtat gttactggga aattttgaac
    21361 aaggcaatcg ggtttatgag ataactggaa cagcgcctac tttgtcaacg aaacaaggcg
    21421 gaacgaagat catgcttcgg aaaaacgaaa cagaatatca agaggtgaag ccagggaaca
    21481 gtgtgaacct ggcttttcct aattccacaa gtcgtcgagg ccgtctggga aaacaaagtg
    21541 tgcatacctt attaacagga gaccaacaag cagtcgtcac ggatcagtat caaattcgaa
    21601 agttaacacc tagggagtgc tggcggcttc agggctttcc ggactgggct tttgatcgag
    21661 cttctcaagt caattcagat agtcagttgt ataagcaggc tggaaattct gtaacagttc
    21721 cggtcatttt tgatattgct agaagattaa aggaggtaaa aacagatgat ttatgatgag
    21781 ttgattgggg aatctactgg gtgattggca agattcagtc agatccggaa ttggaagaag
    21841 aactgtaccg gctcaatttt gatattcgca aaaacggggt gaaagtccct gatgatccgt
    21901 atgtaattga tgaggaaacg gatgctcgaa tggagctgaa tcaagtcatc agtgaattgg
    21961 aacgaattgc ggactttgct aaagaacctg atatccgcca atacttgttt gagataaaag
    22021 cagagttgga aattgagggt attaccgcgg agtaacggcc aaataaacca ctgataggca
    22081 ctgaccgcgg gggatttgcg aagcagaaat cctccgtggt gctgtgcctg tttatggacg
    22141 gcgttagccg gccatgatgt ctcacccccc gtatctaaca ggggggtaca aaaacacaaa
    22201 aaataagcaa gcaacgataa ggaatggctg tcctatcaag gataataagc aatttgtgta
    22261 gatgttaact gatttgaaaa agttaacacc ttaaatttgg aggtgagaac ttggcacaaa
    22321 ggaatttaga ttatcggcta ttaaaagacc ggcgtaatga atatggcgtt tcacaaaata
    22381 aattagctac gacttgtgga cttagtcgcc cgtacttaaa tcagatagaa aatggtggcg
    22441 taaccgcatc gacaaaaacc atgaggaaaa tttttgacca actggaaagt ttcaatcccg
    22501 agttaccttt aacgttactg tttgactatg taaggattcg ctttcccaca acggatgcga
    22561 ggaaaatcat tcaagagatt ctccatttga aatttgatta tatgctccac gaagattacg
    22621 ccttttactc ctatcaagaa caatatgtta tgggagatat tgtggtgatg ttgtcacatg
    22681 aagaagataa aggcgtcctt ttagaattaa agggtcgtgg ttgtcggcag tttgaaactt
    22741 ttttactcgc ccaaaagcgt agctggtatg actttttcga agattgtcta aaagccggtg
    22801 gcgtgatgaa acgcttagac ctggcaatca atgatcgagt tggcctgtta gatattcctg
    22861 atttaacgaa aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc ttcaaaagct
    22921 atcgttccgg ggaactgttg aaacctgatg ataaagatgg tatgggcaat actttgtata
    22981 ttggcagtct taagagtgag gtctatttct gtttgtacga aaaagactat gagcaatata
    23041 tcaagttagg gattccttta gaccagaccg aaacgaaaaa tcgttttgaa atccgactga
    23101 aaaatgatcg agcttatcat gcaattcaag atttgttaaa aggtcgcagt attgaaagta
    23161 ccacattttc cattattaac cggtacttgc gatttgcgga taaggtggaa ggcaaaaggc
    23221 gaactaactg gcccttgaat gaacaatggg ggcgcttcat tggacggaat cagaaggaaa
    23281 ttcaattaac ttctgaaccg aagccttata ccattgaacg aacgttgaac tggcttggac
    23341 gacaagtcgc ccctacgtgg aagatggcca aggagttgga tcggttgaat cagacaacgt
    23401 acattcagga tatggtaaat aatactcgac tatcagaccg gcacaaaaag attttggagc
    23461 aacaaagtat ggcaattgaa aatttgatta tatgaaaaga ggaatcgtga tggaaattgt
    23521 gaaagatatt ttattggtgt ttggtggcag tatgcttggt gttactgtga tgtgcttgat
    23581 gcatgcaagt gcagcggcgg accgagcaat ggaaaagaaa ggaaagtgat gaaatgaatt
    23641 tcggtcaaaa ttt
//
LOCUS       DAZKOR010000042        23496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000042 DAZKOR010000000
VERSION     DAZKOR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.042"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..1774
                     /locus_tag="ACG8ZM_001539"
     CDS             113..1774
                     /locus_tag="ACG8ZM_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0492504.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1844..2191)
                     /locus_tag="ACG8ZM_001540"
     CDS             complement(1844..2191)
                     /locus_tag="ACG8ZM_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0492505.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2275..3216
                     /gene="opp3b"
                     /locus_tag="ACG8ZM_001541"
     CDS             2275..3216
                     /gene="opp3b"
                     /locus_tag="ACG8ZM_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0492506.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3217..4260
                     /locus_tag="ACG8ZM_001542"
     CDS             3217..4260
                     /locus_tag="ACG8ZM_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0492507.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4266..5336
                     /locus_tag="ACG8ZM_001543"
     CDS             4266..5336
                     /locus_tag="ACG8ZM_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492508.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5326..6279
                     /locus_tag="ACG8ZM_001544"
     CDS             5326..6279
                     /locus_tag="ACG8ZM_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492509.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6291..7301
                     /locus_tag="ACG8ZM_001545"
     CDS             6291..7301
                     /locus_tag="ACG8ZM_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0492510.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7586..8407)
                     /locus_tag="ACG8ZM_001546"
     CDS             complement(7586..8407)
                     /locus_tag="ACG8ZM_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0492511.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8504..8782)
                     /locus_tag="ACG8ZM_001547"
     CDS             complement(8504..8782)
                     /locus_tag="ACG8ZM_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492512.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9050..11059
                     /gene="metG"
                     /locus_tag="ACG8ZM_001548"
     CDS             9050..11059
                     /gene="metG"
                     /locus_tag="ACG8ZM_001548"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0492513.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11538..12125)
                     /locus_tag="ACG8ZM_001549"
     CDS             complement(11538..12125)
                     /locus_tag="ACG8ZM_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492514.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12300..13622
                     /locus_tag="ACG8ZM_001550"
     CDS             12300..13622
                     /locus_tag="ACG8ZM_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0492515.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13777..14553
                     /locus_tag="ACG8ZM_001551"
     CDS             13777..14553
                     /locus_tag="ACG8ZM_001551"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0492516.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14546..15115
                     /gene="rnmV"
                     /locus_tag="ACG8ZM_001552"
     CDS             14546..15115
                     /gene="rnmV"
                     /locus_tag="ACG8ZM_001552"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0492517.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15108..15992
                     /gene="rsmA"
                     /locus_tag="ACG8ZM_001553"
     CDS             15108..15992
                     /gene="rsmA"
                     /locus_tag="ACG8ZM_001553"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0492518.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16443..17072
                     /locus_tag="ACG8ZM_001554"
     CDS             16443..17072
                     /locus_tag="ACG8ZM_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492519.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17196..18305)
                     /locus_tag="ACG8ZM_001555"
     CDS             complement(17196..18305)
                     /locus_tag="ACG8ZM_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492520.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18514..18936
                     /gene="mgsA"
                     /locus_tag="ACG8ZM_001556"
     CDS             18514..18936
                     /gene="mgsA"
                     /locus_tag="ACG8ZM_001556"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0492521.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19123..19221
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19259..19795
                     /locus_tag="ACG8ZM_001557"
     CDS             19259..19795
                     /locus_tag="ACG8ZM_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0492522.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19835..21598)
                     /locus_tag="ACG8ZM_001558"
     CDS             complement(19835..21598)
                     /locus_tag="ACG8ZM_001558"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492523.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21601..23316)
                     /locus_tag="ACG8ZM_001559"
     CDS             complement(21601..23316)
                     /locus_tag="ACG8ZM_001559"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492524.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7579 a         4297 c         4777 g         6843 t
ORIGIN      
        1 ttatgaaaaa gacaaaaaaa agtgttgatt ttttaataat tattcattaa actcttaaac
       61 aacttcagaa aatattaatt attctgaaaa ttccaataaa ggggtacgta aaatgaaaaa
      121 gaaatttaca tttggtttgg ttgcggtatg cggccttgta cttgctggat gttatggcgg
      181 cagttcagcc acaagcagta gttcagcaaa aagcggttca gcagatggag gaggcgtatt
      241 taacttagtc gtgcctcaag aaatgcctac agctgactta tcagttgcaa cagatacaat
      301 cagttttaca gcattgaata acgtttatga aggaatttac cgtcttgatg aagacagcaa
      361 accacagccg gcaggagcta gcgagttggc agaagtcagt gaagacggat taacttacaa
      421 aatcaaatta cgagaagacg caaaatggtc aaatggtgat cctgttacag cagcggatta
      481 cgtttatggc tggcaacgaa cagtagaccc agctacagct tctgaatatg cttacctgta
      541 tgcgccagtt gaaaatgcag aagacattac agctggaaag aaagataaat cagaactagg
      601 gatcaaagca gttggtgact atgaattaga aatcaaattg acgaaacaaa caccatattt
      661 ccaatatctg cttgctttcc catcattttt cccacaaaaa caatccgtag tggaagaaaa
      721 tggggatgcc tatgcatctg caagtgataa agctgtttat aatggaccat tcacattaga
      781 agattttgat ggaccaggaa cagatacaga atggacttat aagaaaaatg atcattactg
      841 ggacaaagat acagtaaaac tttctgaaat caaagtcagt gtagtaaaag aatcttctac
      901 tgcattgaac ttattcaaag atggccaagc agatgacgtg atcttgtctg gtgagttagc
      961 acagcaaaac gcaaacgatc cagcttatac ttctgttaaa gaagcgcgga caagctatat
     1021 cgaattcaat caacgtgaaa agaattcacc attcaacaat gtgaacctaa gaaaagctat
     1081 ttcttattct atcgatcgtg aagctttagt aaaacaagta ctaggtgacg gatcagttgt
     1141 ctctacagga ttgatcccat cagatatggt aaaaagtcca acaacgaacg aagactttgc
     1201 aaaagaagct ggaaaattag tttcttatga caaagataaa gcaaaagaat attgggaaaa
     1261 agcgaaaaaa gaattgggcg ttgattcttt agaatttgat ttgatggctt ctgatgatga
     1321 ttcatcgaaa aaagtaatcg aatatgttca aaactcaatc caagaaaact tggatggcgt
     1381 aacagtcaaa ccaacacctg ttccattctc tgtacgtctt gaccgctcaa cttcgggtga
     1441 ttttgacgca gtactaggcg gatgggctgc tgactatgca gacccaagca gctttactga
     1501 tttgtttgtc acaggtaatt catacaaccg tggtcgctgg agcaatgcag aatatgacga
     1561 agcagttaaa gcagcttcat caacagacgc tggcgatgaa caagcccgtt gggatgattt
     1621 ccaaaaagca atcaagatca tctctgatga tatgggtgtc gttccggtgt accaaaaagc
     1681 agaaggtcac ctagtgaatg aaaaaatcaa aggtatcgtt caccatgcgg caggcgcttc
     1741 atgggattac aaatggacat acgttgaaga ataatcgtta ataccgtgag catgagaaaa
     1801 aacgaggctg cggcaaatgt cgcaacctcg ttctcatatt cacttatcct aacaaagaaa
     1861 aaagcatgaa aatagcagaa agagcaatta gtattccggc aatgatcagc caaaagctgt
     1921 acataccgta gctggcagac gagagcgtgg aaaattctgg ttttttcttt cggttccttg
     1981 ttcgtgcaag atggaccgat cgctgaaaat gatcgaagaa acccgaagaa aagacccata
     2041 aaaaaccgcc gataatcaga aacggcaatg accataagaa tagatcatta gataattgaa
     2101 tgagtgtaaa ggaatggttg atgagatttt ttattaaaat aaccagaatc agtagaaaag
     2161 ctactgcgta ggggatagat ttttttttca tttgattgct cctttatttt tggttcatta
     2221 tctaataatg tagcgaaaca tctgtcagaa gtcaaaagct agggggaaac attagtgaat
     2281 agttatatca aatatgtatt aaaacgtgtc tttttcatga tcatcacgct ttggttaatt
     2341 gcaacaatca cctttttctt gatgcaattg ctgccgggca cgccatatac gaatcaggaa
     2401 cgtttgagtc ctgaaacaat cgagatgcta aataaacaag tcggtttgga taaaccagtt
     2461 atcgtacaat atgggattta tctttctaat ttgcttcaag gagattttgg aatttccttc
     2521 caatttaaaa accagccagt cgcacatttg ttagctggaa gaatcggacc atccttgcag
     2581 ttagggttac aggcaattat ctttggaaca gtcctaggga cgattttagg aacgatctct
     2641 gccatgaagc aaaatacatg ggcagataca tcttctacac tcgtagcgat attaggtcgt
     2701 tccattccaa actttgtatt tgctgtttta ttacaatata tctttgcaat caaattacaa
     2761 gttttgccaa tcgcaaaatg ggatggcttt gtgtacacaa tcttaccaac aattgcgttg
     2821 gccatgtcgc cgcttgctga ctctgctcgg tttatccgta cggaaatggt ggaagtactg
     2881 catagtgatt atgtggaatt agcaaaagca aaaggattga gccgttggga aatcgccttt
     2941 aaacatggtc ttcgaaatag tttgattcca ttgatgactt tattaggacc gttagctgta
     3001 gcattgatga ccggatcact ggtggtagaa aatatctttg ctattcctgg tatcggggaa
     3061 caattcgtta agtcaatcac aacaaatgat tatcctacga tcatggctgt aacgatcctt
     3121 tactcattta tgctgatttt tgttattttg atcgtggatc tattgtacgg tctagttgat
     3181 ccaagaattc gtgtttcaga agggagccga ggataaatgg aaatgatacc aaagcgttat
     3241 gaaacagttg cagatatccc tgcggatgaa tttcttccat tacaaaaaaa taccgaagaa
     3301 gaacgtgaac agattactgc accttctttg aattttatcc aagattcatg gcgccgttta
     3361 aagaaaaaca aagctgccgt tatttcaatg gttttactat tggtcatcat tttgatctct
     3421 gtgatcacaa tctttgtttc tcctcatgat ccaactgccc aaaacgtggc ttatatcaat
     3481 ttgcctcctc gtattccggg aatcaatatc gatggattga atggaaaagc aatggttggt
     3541 ggtgaacttg ttgataaata tgctcaagca aacgtccctt caaacgtaaa tttcttttta
     3601 ggaacagacg gtttgggacg tgatgtatta agccgcttat ttatgggaat gagaatctcg
     3661 ttgctgatcg catttattgc tgcattacta gatgtaacga ttggtgtagc ttacgggttg
     3721 gtttctggtt tgttaggcgg acgtgtcgat aacgtgatgc aacgtttttt ggaaatctta
     3781 tcaggaattc caaacctcgt tgtcatgatt ttgatgttgg tcgtttttga accaggaatt
     3841 ttttcaatcg tggctgctat ggccatcaca aactggatac caatggcacg tatcgttcgt
     3901 gcacaaacaa tgaaattaaa agatcaagaa tatgttttag ctggattgac attaggtgaa
     3961 tcaagaatga aaattgcatt caaacatatt ttgccgaata tctctagtgt cattattatt
     4021 caaatgatgt ttagtattcc atctgctatt ttcttcgaag cctttttaag ctttatcggt
     4081 ttaggtctta cgcctccgtc agcttcatta ggaacgatgt tgagcgaagg gtacaagaca
     4141 ttcctgtatc tgccttatct attatggatt ccggcagtaa ctttatcggt tatcatgatt
     4201 ggctttaact tacttgctga tggattgcgt gatgcctttg atcctaaaat gaaagagtga
     4261 agacgatgac gaaaatttta gaagtgaaag acttagaaat ctcttttgat acgtatgctg
     4321 gaaaggtacg agcgattcgc ggtgtcaatt ttcatttgaa taaaggagaa acattggcga
     4381 tcgttgggga atctggaagt ggaaaatcag tgacgaccag aagcatcatg cgcttgctgt
     4441 caagcaacgc caatatagat gctggtcaaa tcctctttaa aggtaacgat atcgttggaa
     4501 aatcagaaag agagatgcaa aaaatccgtg gtaaagaaat agcgatgatt ttccaagatc
     4561 caatgacctc tttagatccg acgatgccca ttgggaagca agtcgctgag tcccttagaa
     4621 agcataataa agtttcaaaa aaagagggac aaaaagctgc attagaattg cttaaattag
     4681 ttggaatacc cgaagctgag aaacgtatca acagttatcc gcatcagttc tcaggaggtc
     4741 agcgtcaacg gatcgtgatc gcgattgcac tggtatgtta tccggaaatc ctgattgctg
     4801 atgaacctac aacagcgctt gatgtgacta ttcaagcaca gattttggag ctgctgaaag
     4861 aaattcagac aaaaatcgat acatcaatta tctttatcac tcatgacctt ggtgtagtag
     4921 cgaatgtcgc tgatcgtgtc gctgtcatgt atggaggaag aatcgttgaa gttggaacat
     4981 cggaagaaat tttctacaac ccgcaacatc cctatacttg gggactgctt ggttcaatgc
     5041 cgacattaga tagtgcgaac gatcgattgt atgctattcc tggttctcca ccggatttat
     5101 tgaacccgcc aaaaggagac gcattctatc caagaaatga attcgcgatg aaaatcgatg
     5161 cagaacaaga accaccattt tttgaactat caaaaacaca caaggcagca acttggctac
     5221 ttgctccgca ggcacccaaa gtgacaccgc cagaagaaat ccaacgtcgc tgggctatct
     5281 ttaaagagaa acaaaaacat acttacggtc aaggaggaat tgctgatgcc aaataaacta
     5341 cttgaagtta aaggattgaa acagtatttc aatgtaggaa aaaagaatga agtacatgct
     5401 gtaaatgaca tcagctttca catttatgaa ggagaaacat tcggtttagt tggagaatct
     5461 ggaagcggaa aatccactac tggccgtacg atcattcgct tgaatgaacc tactgatggt
     5521 gagatattat tcgacggtca agatataacg aagatcaaag ataaaaaagg tttgacgaaa
     5581 ttccggcatg atgttcagat gatctttcaa gatccatacg cttcgttgaa tcctcgtatg
     5641 aaagtcagag atatcattgc cgaaggaatt gatgttaatg gactagctaa atcatctgaa
     5701 gagcgtgcaa aaaaagtcga tgatctattg agaactgttg gattgaatcc aagccacggt
     5761 actcgttatc ctcatgaatt ttcaggtggg caacgccagc gtattggtat tgcacgagca
     5821 ttggcagtaa aaccgcgttt tatcatttgt gatgaaccaa tttctgcact agatgtgtcg
     5881 atccaagccc aagtggtcaa tttgttgcaa gatctccaaa aagaacatca attgacctat
     5941 ctatttattg cacatgattt gtcgatggtt aaacatatta gtgaccgaat cggtgtaatg
     6001 cataacggct tgttattaga gatgggaaca agtgatgaaa tctataatca cggtgttcat
     6061 ccttatacag aaagtttgtt atctgctatt cctctaccag atccagacca tgagagaaaa
     6121 cgcagaagaa tcaaatatca acctgaacca gatgacggac aagtacgtca acttcgtgaa
     6181 atcgcaccag aacattttgt ctatgcaact gagcaggaag tcccatatta cgcaaaaaaa
     6241 ctaaaacgac aaaaggaatc tcttctggtt gctaactagg agggacatag atgctaaaag
     6301 cttttaaatt ccgtatgtat ccgacagaag aacaaaaaca gcaattgatt cgtacttttg
     6361 gatgtgctcg atttacctat aaccatttgc tgaaaaagcg gcagaaaagt tggcaacaaa
     6421 caggagtagc caatttttcg ctcacacccg ctacattaaa aaaagaatac ccattcttaa
     6481 aagaagtaga cagtcttgct ttagcaaatg cacagcttaa tttggatcgg gcttttcgca
     6541 attatttcaa gggacgtgcc agcttcccaa agcttaaaac gaaaaaaagt atgtggcaat
     6601 cttatacgac caacaaccaa acccgtacga tctatctgga aaatggctac ttaaagttac
     6661 caaaacaaaa agaactaatc aagataaatc agcatcggcc agttgaagga tcgattcgct
     6721 ccgctacgat ttctgctcga tataatgaag aattttatgt cgcactactt tgtgatgttt
     6781 cgccagtaaa aaaagaatct ttagctaaat ggatcgggat cgcctatcat ccgaagacat
     6841 tgatccaaac atctcgacct ctagaagtaa ctttaccaaa atttcaccag acagaagaaa
     6901 agctgcaaca tgcacagcgg aaattgaatg tgaaagtccg ctccgctcac catagaaaga
     6961 tcaggttaga tcaggcaagc aattatcaga aacaaaagag aaaagtaatg gatctttatc
     7021 tgaaacaaaa gaaccagcga gaagattatc ttgaacaatt atctgggaaa ttggtcaaac
     7081 aatatgacta cttatttgta gaatcttttc caaaagaaga ggcacatgct gatttttcta
     7141 ttcacgactg gcataaatta ataacaaaat tgcgttataa gtcccaatgg tataataaaa
     7201 aatttctttt gatcaatacc gacggagcag aagaaagcaa ttccgtaaga aagagccaag
     7261 tagtagaaaa aatgggcaga cattcggtta tcaagggata aaaaatgaat gaaactgata
     7321 ggagcgttag ggttagcctt gttaataaaa agtcttaatg acttttgttt ctaggaattt
     7381 ttcttttgaa gaaaagctca ctaattatga caaaccaata aaacaatagc agaaaaagtt
     7441 cgtacgagct ttttctgcta ttgttaattt tttattcaga aagtttacga atacatcaaa
     7501 attctgaaac aaaaaacgta actcacaaag agttgttttt gcgttttttc ttgaatgatt
     7561 aggtctgaat cttttgctca caaccttatt catggtgccc cggcttgagc tgatagattt
     7621 ttgaaggacg gccgattcct acaggacgtt caccgatctc atcgaaatat tggagcatag
     7681 ctttcttaaa attggaatga tcgatcgttt tataatcgat ccctaagaat ttagcaaata
     7741 cttttcgagc ttccgtaatc gtgaaatctt tacctaaaac ttgtagaact tgcggctcgt
     7801 gttccatttt attgactaca cggttgaatg ctttcaagat gatttgacta tggtcaaaag
     7861 ccaaagtatc tttgccagaa gactctccag tgactaaatc aagcgagata gccacttctc
     7921 cgctagataa atggatcttg ttactatgac gttctagtgt gaaccagcgt acttccttgg
     7981 catcatctcc ggcgattagc ggttcttctc caataaaggc taagtagctg actgtgacga
     8041 cccagcctcg cggatctcga ttaggcgtac tgaatgtatg cagttgctca atattttctt
     8101 ttgaaataac cacaccagtt tcttcctttg tttctcttaa gacgctttct ccagttgact
     8161 catccttttg gacaaagcca ccaggcaatg cccatgaatt tcgataagga tgcccttttc
     8221 tttggatcaa caaaactttc agctgatctt cttctttgtt atagcacatc agtacgatat
     8281 cgacagtcaa agagggcttg tcatattctg gacgttcctg tttttgatac cagttaagaa
     8341 attcttcttc acttgcttta tgttcataat ataatttttc ttcttcttta gaagcaaaac
     8401 ggttcatctg accacctcca ttgacagtaa aagtactttt taccttattg tagcagactc
     8461 tgaaaaaaaa ccaaggattt acttgaaaaa tctaggttat catttaaata tgaaaaataa
     8521 atggaagtat gagattgaat aaaaagaaaa gcagataaaa cggcaatact ttataccgct
     8581 cttcacgaat ggcaaagaag ccacaaataa cgatatatac gtcaatgata aaacgcaaaa
     8641 tccaagctgc tgtcataaaa ctgttcaaat gcccatggag gttaaaataa ccatacatac
     8701 gaatataaaa atagctgaat attaaatgtg ctaatgaaag aaaagcaata ctaagtgtaa
     8761 aaaaaagatg tttcttctgc atgattgttc cagtccctta ctaagataga tagtctttat
     8821 tataccacgt gtgaggggag aaataaaaag ccgtttttta gtagatatga aacaaaacaa
     8881 aaacccgcat ttattcaacc tattcatcct tttaaaggat agattgaacg tagatagaag
     8941 tcgtttatcc agtctttttc attttgaaaa aaagctaaca gcatgctttt tttatggtat
     9001 actagcaagg aaacgaattc gcatcatgcg ataaaggagg aagagccccg tggcagaaaa
     9061 ggaaactttt tatatcacta cgccaatcta ttatccaagc ggcaaacttc atatcggcaa
     9121 ctcgtatact acgattgcat gtgacgcaat tgctcgttat aaacggttga tgggctttga
     9181 tgtgttttat ctcactggag tggatgaaca tggtcaaaaa atcgaaacaa aagcagaaga
     9241 actaggtgta aaaccgcaag aatatgtaga taaaatggcg gctgatgtca agaaattatg
     9301 gaaaacgctt gatatcagtt atgataaatt cattcgcacg acagatgatt accataaagc
     9361 agctgttcaa aaaatatttg atcgtttatt agaacaggga gatatttact taggtgaata
     9421 tgaaggctgg tactctgttt ctgacgaaga attctttaca gaaacacaac ttgcagaagt
     9481 gtacaaagat gacgaaggta acgtgatcgg tggtaaagcg ccaagtggac acgaagtaga
     9541 attggtaaaa gaagaatcat acttcttccg catgagcaaa tacgcagacc gtctattgga
     9601 atactatgaa gaacatcccg aattcatcca gccagaatct cgtaaaaacg aaatgatcaa
     9661 caactttatc aaaccaggat tagaggatct tgcagtttca cgaacaactt ttacttgggg
     9721 agtaccagtc aagagtgatc cgaaacatgt ggtgtatgta tggatcgatg cattatccaa
     9781 ttatattact gcactaggat atggctccga agatgaaagt ctattcgaaa aatactggcc
     9841 tgcagatgtt cagatggtcg gtaaagaaat cgttcgcttt catacgatct attggccaat
     9901 catgctgatg gcccttgatt tgcctctacc taaaaaagta tttgggcatg gctggctatt
     9961 gatgaaagac ggaaaaatgt ctaaatcaaa aggaaatgtc gtttatcctg aaatgcttgt
    10021 ggaacgttat ggtttagatg cactccgtta ttatttgctt cgtgctgttc cgtttgggtc
    10081 agatggtgtc tttacgccag aagattttgt ttcccgtgtc aattatgatc tggcaaatga
    10141 tctagggaac ttattgaatc gaacgattgc tatgatcaac aaatattgtg atggactcgt
    10201 tcctgactat gcctcacttg tcactccatt tgatagtgaa ctgtctacga cggctgctaa
    10261 cgttgtcgga cgttaccatg atgcaatgga aaaaatggaa ttcaatacag cattggctga
    10321 aatctgggtg ctgatttctc gtgcaaacaa atacattgat gaaacagaac catgggtatt
    10381 agcaaaagac gaagaaaaga aagctgaatt gaatagtgtc atgatccatt tagctgaatc
    10441 tttgcgtatc gcagcgatat tacttcagcc aatcatgaca gaaacgccag agaagatctt
    10501 caatcaacta ggattggact ctgaaacaat gaatcttgaa ggattacact ttggcgaatt
    10561 tccttcagga acaaaagtag ttgcaaaagg tacaccaatc ttcccacgtt tagatatgga
    10621 agaagaagtt gcttttattc aagaaaaaat gtctgaaggt actcaaacaa acgaagatac
    10681 agtcaaatgg gatccagaag aaacagaact tgtttcaacg aaagaaaaac aaatcaaatt
    10741 tgatgtattt gaaaaagtag aactaaaagt agcagaagtc atcaactgtc aaaaagtaga
    10801 aggggcagat aagttgctgc aattccgact agatgctgga gacagccaag accgtcaaat
    10861 cttgtcagga atcgctgaat tttatcctga tccaagcgaa ttgatcggga aaaaagtcgt
    10921 cattgtagca aatctgaaac cacgaaagat gcgtggacaa atcagtcaag gaatgatcct
    10981 ttctgcagaa gcaccagatg gctctttaca agtaatcgaa gcaccaaaat ccatgccaaa
    11041 tggctcagaa atcgcataga aattcaaagg gggtatgaca ctagttgtgt cataccccct
    11101 ttttccgaaa tttgagaagc aattttcgta aattccttct tattacttga agccaatcat
    11161 ataatacaga aacagagaac tatccaaaat aaatcctaga aaaagaagta cgagcttcgc
    11221 tgatttttgt taatttagaa taatactaat aggaatcgta aaaacagaaa aaaagcataa
    11281 tgataaaggg tcgggcagat tttaattgat tcatctggta ctctttttta ttgtccttac
    11341 attcataaag tttgaatgca atgactttat tacatctata tttatatcta acagtagcgt
    11401 tgatgtgtta attataagtt ctatcacttt atgttcaatt agtttgtgct cttatatgtg
    11461 tgattgaaaa tatagataaa aaaacaatca tcggctttga cttcgatgat tgttttcttt
    11521 attttgtaaa aatcctatca cttgatttta tggtaacctt ttatttttgt cttcaaacgg
    11581 cttggtaaga gagcagtaag cagtgcaaat gggatcgaaa agacatacca gaaaatcgta
    11641 cgtccgatac cataatgttg atagagcttt gtcaacatca cgtgtaacaa gatcaaccct
    11701 aaaagcaagg agccagctat aaaccaatga ttatcccaat gcatgacttc actaagtgcc
    11761 atcactccaa aaggatagaa ataaaaatag atcccgacta aggtcatccg ccagttcttt
    11821 gtaaatgttc cgatattcaa taaaaagaaa atcgctgaaa gcaaaatacc gataggcgga
    11881 aaaagcccaa ccaaaaagga atacaacatc ccccacaaga tcatcacagg acctttgatc
    11941 aatgcaacga ttgcgatgaa caaagtcagc aatattagtt tttgtgataa cgttgcaaca
    12001 gtcattaatc caaataaaat taatgtgaac caatgccaat gggactgctg ttgttcttta
    12061 cgggaaaaat cgacccggtt ggataaataa tctgctaatt tcggataaga acgcttaaaa
    12121 tccataaact gttcctcctt cgtggaaaag gtaggtttag tatacgaaaa ataaaaaaga
    12181 aatacatccg tcttaagagg gagtttttca ctgaaagtgg agaaaaaaga atggtaaatt
    12241 aaaatattct cagttatact gaaaattata ccaaatttca aagaagaaag aggatgggaa
    12301 tgaaaggcaa actaaaacga gaaatcaatc tgtttggcgc attagctacc gttatgggaa
    12361 ccgtgatcgg tgcaggcgtc ttcttcaaaa ctgctgctgt tactgctagt actcagtcta
    12421 caagtttgac attgctagct tggcttttgg gtggtttctt aacgatctgt gctggattga
    12481 cagtggctga attagctaca gcaatcccgg aaactggtgg agctgtaaaa tatattgaag
    12541 ctgcgtatgg aaaattgccc agctttttat taggatgggc gcaaagcttg atttactttc
    12601 ctgctaatat tgctgcatta tcgattatct ttgcgacaca attgacgaac ttattacaat
    12661 tatctaccga ttatcttttg ttgattgcag tgatcaccgc tgtttctgtg acagggttga
    12721 atttgttagg gacaaaagtc ggtgcttctg tacaatcagt gacgttgatt gttaaattaa
    12781 taccaattgc tgtgattgtc atatggggat tattgacccc aggacaagga actgtccagt
    12841 tatttccaat cgaagctgga aaagatgtta cttttgtcga aggattgagc agtgcgttgt
    12901 tagcaacgtt gtttgcttat gacggctggt taggtgtcgg tgcaatggca ggggaaatga
    12961 aacggccgga aaaagatttg cctaaagcaa ttattcttgg tttgagtttt gtaacagtcg
    13021 tttatctatt gatcaatttt gtttttttga aaactttgcc gattgatcat cttgccggta
    13081 atttgaatgc agcttctgaa gcatcagacg tcatttttgg aggaatcggt ggaaagctgg
    13141 tcactattgg gatcttgatc tcggtatacg gtgcattgaa cggttataca ctaactggga
    13201 tccgcgtgcc ttatgcgatg gctttagaag acgagctacc tttcagtaaa cagctgacaa
    13261 agctttcaaa gaagtttacg gttccttatg ttcctgcggt tttccagtta gcagtagctt
    13321 gtattatgat gagccttggt tctttcgact ttttaacaga catgttgatt tttgtgatgt
    13381 ggctattcag tttactgatt tttatcggtg tctttatttt aagaaaaaaa gcgccggaat
    13441 tgccacgtcc atataaagta ccactttatc caatcgttcc gattattgcg attctcggtg
    13501 ctattttcat tttaggtatg acgatgatga cgcaaacgaa attagcattg atcggtatag
    13561 gtgttacctt gataggtatc ccagtctatt atcaaaaaaa gaaaaaacgc tcagaagagt
    13621 aaaatatttc aacaacagac agaaaaagta cgaactaggt cctctagatc ttcgtgcttt
    13681 ttctatttca ataaaacaag ttcttctatt ttgatttttg ctacttctgc aagtagtgct
    13741 tatgtgctaa agtaagaatg aaaaggagag ataaacatga tatttgattc tcatacccat
    13801 ttaaatgctg aacagttcaa tgaagatatt ccagaaacga ttcagcgagc acaagaaatg
    13861 ggggtaacga aaatggcggt cgtgggcttc gatacgccaa cgattgaaaa gtctttgcaa
    13921 ttaagccatg actatccaaa tatatacagt attatcggct ggcatccgac agaggctggc
    13981 agttatacga aagaaatcga aaataaatta caagaacaat tgactttacc aaaggttgta
    14041 gcattagggg aaattggctt ggattatcat tggatggaag acccgaaaga agtccaagat
    14101 aaagtatttc gcagacagat tgctatcgca aaagaaatga acctaccaat cagtattcat
    14161 actagagaag cacttgaaga cacgtatcaa atcctaaaag aagaagatgt tcgagatatc
    14221 ggtggaatca tgcatagttt cagtggtgat tatgaatggg caaaacgatt tcttgactta
    14281 gggatgcata tttcatttag cggtgtcgtc actttcaaaa aagcattaga cgtccaagag
    14341 gctgcagcaa atgtaccaat cgaacgatta cttgtggaaa cagatgcccc ttatctggca
    14401 ccagttcctt ttagaggaaa gcgaaatgaa ccaggctata ctcgttatac agtagaaaag
    14461 attgctgaac ttcggaatct tccgacagaa gaagtggctg cccaaacatg gacaaatgcg
    14521 caccgattat ttggtctaag cgaacatgga taagatgaag atccaagaga ttatagtcgt
    14581 tgaaggaaaa gacgatacaa gaaggctgcg ggaagtagtt gatgcagata cgatagaaac
    14641 aatagggtct gcaatcaatg aagagatcct gatgcaaatt gaacatgccc aagagactcg
    14701 tggagtgatc gtttttaccg atcctgattt ttcaggagaa aaaataagaa aaatcattat
    14761 ggaagctgtt cctcaggcaa aacatgcttt tttgccgcgc acacaagcta aaccaaagaa
    14821 aaagggaagc cttggtgtcg agcatgcaag tgatgctgct attttagaag cattgaaaaa
    14881 ggtcgtgacg ccagacattt cccaaacggc tgtttctgaa atcactagac aagacctttt
    14941 agactatggt ttaatagctg gggcacaagc aaaagaaaaa cgagaaaaac taggtgacga
    15001 gttgcgcatc ggttacacga acggcaaaca attggaaaaa cgtttgaaga tgtttcgtat
    15061 tacgccgaaa gaactcgccc aagcaatgaa aatagtggag gaacaacatg agtgaatatc
    15121 gtgaaattgc gacaccctct agaacaaaag agattttatt gaaacatggt ttttcattta
    15181 aaaaaagttt aggacaaaat tttctgactg aaccaaatat tttaaggaaa atcgtagaaa
    15241 ctgcgcaaat agatgaacat acgaacgtga tcgaagttgg accaggaatc ggtgcattga
    15301 cggaacaatt agccaagcat gcaaaacaag tcgtagcatt tgagatcgat gatcgtttga
    15361 tccctgtatt ggctgatacg atgcaaccct atgacaatgt gactattgta catcaagata
    15421 ttttaaagac cgatttgtca acagctgtta gagaaacatt tcatgaggag cttccgttaa
    15481 aagtcgtagc gaatttacct tactatatta cgacaccgat catgatgcat tttcttgaat
    15541 cagatttggt tgtggatgaa ctagtcgtca tgatgcaaaa agaagtagct gatcgtatct
    15601 ctgctgaacc aggaacgaaa gcttatggtt cattatcgat tgctgttcaa tattatatgg
    15661 aagccagttt agcattcatc gtaccaaaaa cagtatttgt ccctcagcca aatgtagatt
    15721 cagcgatttt gaaactgaca agaagagata taccggcagt cgaagtgaca gacgaaaaag
    15781 catttttccg attgacaaaa gcggcatttc agcaaagaag aaaaacgctt tggaataact
    15841 tgcagcattc gtatggcaaa gatgaccaga caaaagcatg gttagcaaaa agcttggaaa
    15901 cagctggaat cgatccaaaa cgtcgtgggg agactttgtc attacaagaa ttcgccgcat
    15961 taagcaatgc gatgtcagaa aatatgcagt aaaaaaagag gttgtgacag aagttgtgaa
    16021 aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catgtttttc
    16081 gtttgtttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag aaagaggttg
    16141 tgggcctgcc gtttaactct aagtcacaag gaacaatttc aaaaaacagc ttctcatgtt
    16201 tttgagttag ttggtcttat tacttgatgc aagcttttga ccactattat gccgaacaag
    16261 gagatgtgac acaaattttg tcacatctcc tatttttgtt tcttgatcaa aaaattctta
    16321 tacttgatac tcacactcat tttgtatgga tttagaaaag aatatgtgtc ttgacagtta
    16381 ttagtaagtg agttatacta agtcgacatt tatgtgtcaa gacatattag gagagagaaa
    16441 aaatgagaaa aaattcagtt cgaaaactaa cgatttctgc tttattgatt gggatggggg
    16501 tcattatccc aatggtcatg cctaaaatca tgattccacc agcttccttt actttggcgt
    16561 ctcatgtgcc attatttatt gcgatgtttt ttacaccagg agttgcagtg gctgttgcgc
    16621 taggtacgac atttggtttc tttttaacaa cgccggttat catcgcattg cgagcattgt
    16681 cacatcttgt gtttgcgttg attggggctt ggtacttgca aaagcatccg agtatcgttt
    16741 taaaaaatgg acagtttact tttgctaatt ggcgtttcca agggttcaat tttgtgatcg
    16801 gtgtgattca ttctttagca gaaatgctag tagtcagcat cttttacttt attggtaata
    16861 tgcctgaaac atattattct caaggatatt tttatacagt attcatcttg atggggattg
    16921 gtgggcttat ccacagttta gttgattata atattgctta ttttatagca ggaacactca
    16981 gcaaatattt tgatatcccc gtatttacgg cagcaaaaaa agaaaatcgg aaagagtcga
    17041 ttcaagggta cgcgaagaaa acgacatatt agacatgatc tctcaaaaaa aaaactctca
    17101 tctagtaatg gataataaat cctctaagat gagagttttt tgtcctaaaa agagctgcac
    17161 caacatcttc tggcacagct ctaacagggg gcaatttatg gaagtttgat gacttgttct
    17221 gtttctttat caaagaagtg gcctttgctg atgttgaagg caaggtcgac tatttcgcct
    17281 ggtttatgga aatctcttgc atctactttt gagatgaact cagtagaacc agtttttgtg
    17341 taaagcatcg tttcagctcc aagtaattcg gaaacgacaa cttctgattt cactacggct
    17401 tcaggtgaag cttcaagggc tacttgttca ctgtggatgt cttctggacg aataccgaag
    17461 ataacttctt ttccttcata acctttttca actaatactt tgttttttcc ttcaggaata
    17521 cgaagcttaa gcccatgtcc atctgtgata acaccatttt tcaatgtcac attaaagaag
    17581 ttcatggcag gagatccaat aaagccggca acgaacatat tgacgggtgt atcataaact
    17641 tctttcggtg taccgatttg ttggataaat ccgtctttca tgataacgat tcggtcagcc
    17701 atagtcattg cttctgtctg atcgtgagta acatagatgg tcgttgtttc aagacgctgg
    17761 tgaagtttgg caatctcagc acgcatggct acacgtaatt tagcatctaa atttgataat
    17821 ggctcatcca ttaagaagac ttttgcatcc cgaacgattg cccgtcccaa tgccacacgt
    17881 tggcgttgtc caccagataa agcagctggt tttcgtttca agtattctgt cagacctaag
    17941 atctctgctg cattgtccac acgttttttg atgtctgctt tatcatattt acgtaatttc
    18001 aagccaaaag ccatattatc aaaaacagtc atatgaggat acaatgcgta gttttggaaa
    18061 accatggcaa tatcacggtc tttaggtgcc acgtcattca tgacttttcc gccgatattt
    18121 agttctcctt cagagatatc ttccagtccc gcgatcattc gcaaagtagt tgactttcca
    18181 caaccggaag gaccaacaaa aacgataaat tcacggtctt tgattgatag attaaaatca
    18241 gttacagaat aattttctgc attgtcatat tttttatata cgtttttcag agccatttct
    18301 accataatac gttccacact ttctttttta gttttattaa ctatttaaag tataaatgaa
    18361 agcgttaact aacttcaatg tcatgttgaa caagaatttt ttcttttttt tgtcaatgtg
    18421 ctagttttaa gctttaataa atatccataa gaaataaaaa ctgatacact ggagtaaata
    18481 agtatgttaa caatatcaaa aggagtgtct cttatgaaaa tcgctttgat cgctcatgac
    18541 cgaaaaaaac ctatgatggt gaaattagca acagcgtata aaacaatatt ggaaaaacat
    18601 gaactttttg ctaccgggac gactgggcag aggatcacgg aagcaaccgg cttgccgatc
    18661 catcggttta aatccggacc gttaggcggg gatcagcaaa ttggagccat gatttcggag
    18721 gatcagttag atttggtgat ttttcttcga gacccgctgg ctgcacagcc ccatgaacca
    18781 gatgtaacag cacttatccg tttatctgat gtctatgaga ttccgttagc tacgaatatc
    18841 ggaacagctg aggtgctact aagaggactg gaagccggct tcctagattt tcgaagaatc
    18901 gtccatgaaa tagacaagca tccactttca ttttgatgga taagcgtatg gaaaaataac
    18961 gtttaatgtt cgcctatatg tagctataaa agctaaacaa actgataata tgcgatataa
    19021 tacgaacgta ttggataata taaacaataa tattagtttt tcgtatttgc agtaaatgtg
    19081 aaaatatgct atagtttctg tgggaaaaga tcccaaaaat aatacagagt agaaaataaa
    19141 gcgttaagtg ctaagaggat gggatgttgc ctcttggacg aaggacgtaa tgttcgcggt
    19201 tttattttcg cattcgctgc atgatacgat gtagcagctc ttaaaggaga tgctaaaaat
    19261 gaagaaacac cggttagacg cacgtatgat tgctattatg ggcttattga ttgcattgat
    19321 ggtcactttg tcccgactgg tagcaatcga gaccccattt attaaaatca gtgtcacatt
    19381 tattccacaa gtgatcatgg gcattctttt tggtcctttt tggtcaggga taggcgcagt
    19441 tttagctgat ttagtaggaa tggccctctt ctcgaaatct gcattcttta ttggatttac
    19501 attgaatgca tttattgaag gagcaatcta cggattcttt ttctatcgaa aagagatcac
    19561 ttggaaaaat gccattctgg ctacgcttag tgtcacgctg atcatcaact tatttttgac
    19621 accattatgg cttgcgttga tgtaccatgt gccattgttc agctgggtcg tttgggcgcc
    19681 ccgcctgtta aaaacagtaa tatggttacc gattcaatca attgccattt attacgtagg
    19741 acgtagtatt ccttacaaaa aaattttaag aagtttagcg atccacgcta aataaaaaac
    19801 aagcagtctt cccatcccag aagactgctt gtttttattg tgctgtgaat tgtgcttgat
    19861 agagccgtcg gtaataacca tctttttgaa gcagctcatc atgggtgccc atttcgacaa
    19921 tagctccctg atccatcact aatatctggt ccgcattttt gattgtggac aggcgatggg
    19981 caatcacaaa actggttttt ccttccatca ttgctaagaa ggcttgttgg atttttttct
    20041 ctgtcaatgt atcgactgag ctggtagctt catctaagat caacataggg ggattactga
    20101 tcatcgttcg agcaatcgtc aacagttggc gttgtccatc tgatatcttt aacccttgac
    20161 cgccaatctt agtatctaat ttttccggca aacgttctac aaattcatac atataagagg
    20221 cttttagggc atcattgatt tctgcgtcac ttgcttccgg attcccaaaa cgaagatttt
    20281 ctcgcaatgt actgtcaaat agccaagtat cttgtaacac cattccaaaa cttttacgta
    20341 atgtatcacg ttggatagag cggatgtcat ggtcatcgac tgtaattgtt ccttttgttg
    20401 tgtcataaaa acgcatcagc aaattaacaa gtgtggattt tccggcccct gtttttccta
    20461 ctatagcaat ggtttgacct ggataagcag taaaattgaa atcttcgatc aaacgctgct
    20521 tggggtcata agaaaaagaa acatgatcaa aagagacttt tcctttgata tttttgggtt
    20581 ccaaggcata ggcatctggc aaatcaggct tttcttctgg ctgatcgatc aaatcaaatg
    20641 ctcgttctag accagaaaaa gcggtttgca gttgtgtcgt gattccagaa agttcgataa
    20701 atggtttaga aaactgactt gagtagatcg taaagctgga aatcacacca acagtgattg
    20761 acgaaccact cttcaatgcc aataggccgc caactaaacc aatcgataaa taagcaaggt
    20821 gatctacaaa acgagaagac gggtttgtca aggaagaaga aaattgtgcc cgttgtcctt
    20881 tttcatatag atcagcattt agcagttcaa attgctgttg actagcttct tcttgctgaa
    20941 aagctttcac gattttctgg ttaccgattc gttcggtcac aaagcccgag atatccccaa
    21001 taatcctttg ctgtctatcg aaatttgttt gtgaagcacg agcaacgatc caactcacaa
    21061 ggaaaataat tggtgtagaa caaagaacga ccaatgtcag aagtggactt agacgcatca
    21121 taaaaataaa cgaaagaatg atgattgcca ctccagaaaa taattgattg aaggccgcag
    21181 aaattgcaat ggaaatattg tccatatcat ttgtaaatcg actgacgata tttccgtggg
    21241 agttttgatc ataataacga agtggcaatc gattcagatg agcaaatgca tcttttctta
    21301 attttgcaac ggataagtaa gctaaccgat tgcttaacac ttggatgagc cattgactga
    21361 ctacgttgat taaaacgata ccaccgaata gaataagtaa atggaacagt cgattgaagc
    21421 ggacctgtcc ttctccaatc atttgatcga tactttgacc aatttgtaac gtcatcacaa
    21481 ctgttgcaat cccattgatg attcctagta tcacagctag ccaaatgtct tttgcatagg
    21541 cagtaaggta aggaatgaat cggcggagcg tctgcaagga taattttttt actttcataa
    21601 ctattcttcc tcctgtgatg cgacgatttc ctgatattct gctgattgag caagtaattc
    21661 ttcatgggta ccctgagcag caatcttacc ttgatccatc actaagatat gatgtgcttc
    21721 ttgaatagag cggacacgtt gggagataat aatcactgta ctgccaagat aagtctgtag
    21781 cgccttacgc aaatccaagt ctgtttgata atccaacgca cttaaagagt catctaaaat
    21841 cagtaatgct ggcttggcaa tcaaagctct tgcaatcgtc agtcgctgtt tttgtccgcc
    21901 agagaaattt ttcccatttt ccatgacttt tgtatctaaa ccatttggaa gctgacggac
    21961 aaattcttct gcctgagcga ttttcagagc ttcccaacat tctttttctg tggcgtcttt
    22021 cttaccccat tgaaggtttt cacgaatagt cccggtaaat agtacagaag tttggggaac
    22081 aagagcaatt tgctgacgga gatgatccag ctgccaagaa cgaacatcca ttccgtccat
    22141 tataactgta ccggaactga catcatagaa acgcggaatc aattgtgtca aagtcgtttt
    22201 cccgcttccg gttggaccaa tgattccaag gactgtattt cgcttcagct gaaatgtaat
    22261 attttggaga gaaagaccag aatcaggagt ataacggaag tcaacatggt caaaagcgat
    22321 aattgcttca gaagaaaaat cgggagtagt actgacctca gcttcaagaa tcgaattttc
    22381 tgtgtctagc acctctttca cccgatttcc tgaagcttcg gcacgtgtga agatcacgac
    22441 gaggtttgaa acaacgatca gtgccaaaag catttgattc atataattga tcaaagcaag
    22501 aacttgtcct tgttgcaagc taccaatatt tactttgatc ccgccaactg tcagcaaaca
    22561 gatgatcccg acattcatga tcaaggttgt agcaggtgat aatagggcag agatattgga
    22621 aacacgtaaa taattgttcg ctagatcatt tgtgctttta tcgaacttct ctgtttcttt
    22681 ttttgtctgc gcaaatgcgc ggatgacacg aacaccgcta agattctgac taacgtgttc
    22741 attgagctga tctagttttt cttgcacttt ttggtagaga ggaacggttt ttttgatgat
    22801 aaaaaataaa atgattgaga aaataggcaa taatgctaag aaaaacagac ctacttccca
    22861 gtcgatcaca aaagccatga ccactgaccc gatacttaaa aacggtgctc tgatcaataa
    22921 acgaatgacc attgctaaag ctagctgaag ctgattgatg tcgtttgtca ttcgggtgat
    22981 caaggtatct gttccaaaat gattcaactc gttgtgagag aaagtattga tttttttgat
    23041 tagctgatta cgtaactctg taccaaatcc ttgtgaagct actgaagcat agtattggca
    23101 aatgatagca caaatcagac caatcacaga catagcgacc atccataagc ccatttcaat
    23161 gatttttcct cggtcattca tttttagtcc attatcgatc aaggaagcca tcattaaagg
    23221 caggacaagt tcgaagacag cttcaagaaa tttgaagaac gggcccaaaa tgatttgctt
    23281 acgatagtct tttgcataac gtaataactg aatcatatga ttcctcctat tatttatttg
    23341 tccacaattg aaaaaacaag tactaccaca ttgtagctta ggaaatttag aatgaaaaga
    23401 gaacaagcct tttcataaat gaaagtttta ttatgaagaa gctttagaaa ttgaatagac
    23461 tatttttttt aaaaaaaaga aaatgagaaa acatgc
//
LOCUS       DAZKOR010000043        23357 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000043 DAZKOR010000000
VERSION     DAZKOR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.043"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..994
                     /locus_tag="ACG8ZM_001560"
     CDS             137..994
                     /locus_tag="ACG8ZM_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0492525.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1109..2029
                     /gene="pstC"
                     /locus_tag="ACG8ZM_001561"
     CDS             1109..2029
                     /gene="pstC"
                     /locus_tag="ACG8ZM_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0492526.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2029..2913
                     /gene="pstA"
                     /locus_tag="ACG8ZM_001562"
     CDS             2029..2913
                     /gene="pstA"
                     /locus_tag="ACG8ZM_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0492527.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2928..3734
                     /gene="pstB"
                     /locus_tag="ACG8ZM_001563"
     CDS             2928..3734
                     /gene="pstB"
                     /locus_tag="ACG8ZM_001563"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0492528.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3750..4508
                     /gene="pstB"
                     /locus_tag="ACG8ZM_001564"
     CDS             3750..4508
                     /gene="pstB"
                     /locus_tag="ACG8ZM_001564"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0492529.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4521..5198
                     /gene="phoU"
                     /locus_tag="ACG8ZM_001565"
     CDS             4521..5198
                     /gene="phoU"
                     /locus_tag="ACG8ZM_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0492530.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5436..5735
                     /locus_tag="ACG8ZM_001566"
     CDS             5436..5735
                     /locus_tag="ACG8ZM_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492531.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5776..6468
                     /locus_tag="ACG8ZM_001567"
     CDS             5776..6468
                     /locus_tag="ACG8ZM_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0492532.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6465..7232
                     /locus_tag="ACG8ZM_001568"
     CDS             6465..7232
                     /locus_tag="ACG8ZM_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0492533.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7332..8900
                     /gene="liaX"
                     /locus_tag="ACG8ZM_001569"
     CDS             7332..8900
                     /gene="liaX"
                     /locus_tag="ACG8ZM_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0492534.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9197..9517
                     /locus_tag="ACG8ZM_001570"
     CDS             9197..9517
                     /locus_tag="ACG8ZM_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0492535.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9517..9867
                     /locus_tag="ACG8ZM_001571"
     CDS             9517..9867
                     /locus_tag="ACG8ZM_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0492536.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10034..10972
                     /gene="hprK"
                     /locus_tag="ACG8ZM_001572"
     CDS             10034..10972
                     /gene="hprK"
                     /locus_tag="ACG8ZM_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0492537.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10987..11817
                     /gene="lgt"
                     /locus_tag="ACG8ZM_001573"
     CDS             10987..11817
                     /gene="lgt"
                     /locus_tag="ACG8ZM_001573"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0492538.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11861..12886
                     /locus_tag="ACG8ZM_001574"
     CDS             11861..12886
                     /locus_tag="ACG8ZM_001574"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0492539.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12901..13839
                     /gene="galU"
                     /locus_tag="ACG8ZM_001575"
     CDS             12901..13839
                     /gene="galU"
                     /locus_tag="ACG8ZM_001575"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0492540.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13964..14404
                     /locus_tag="ACG8ZM_001576"
     CDS             13964..14404
                     /locus_tag="ACG8ZM_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0492541.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14407..15009
                     /locus_tag="ACG8ZM_001577"
     CDS             14407..15009
                     /locus_tag="ACG8ZM_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0492542.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15077..16183)
                     /locus_tag="ACG8ZM_001578"
     CDS             complement(15077..16183)
                     /locus_tag="ACG8ZM_001578"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0492543.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16393..17394
                     /gene="ccpA"
                     /locus_tag="ACG8ZM_001579"
     CDS             16393..17394
                     /gene="ccpA"
                     /locus_tag="ACG8ZM_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0492544.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17730..20075)
                     /locus_tag="ACG8ZM_001580"
     CDS             complement(17730..20075)
                     /locus_tag="ACG8ZM_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0492545.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20354..21256
                     /locus_tag="ACG8ZM_001581"
     CDS             20354..21256
                     /locus_tag="ACG8ZM_001581"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0492546.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21469..23244
                     /locus_tag="ACG8ZM_001582"
     CDS             21469..23244
                     /locus_tag="ACG8ZM_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0492547.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT         7443 a         4142 c         4926 g         6846 t
ORIGIN      
        1 agcatagaaa cttttaatga gatagttttt tttacaatta tgagaattta ttttgttatt
       61 taatgatttt gattcgtttt catcaagtaa tgaaaatgat aaaataaaca tactttagga
      121 ttaaaggaga tttctaatga aaaaacttaa attattctta ccaataatag gattagccat
      181 tcttttatca ggctgttcaa agtggattga tcgaggagaa tccattacgg ctgttggctc
      241 ttctgcttta cagcctttag tagaaacagt tgctgaaact taccagacag aaaatcctgg
      301 aaaattcatc aatgtacaag ggggcggaag cggaactggt ttgtctcaag tacagtcagg
      361 agcagtcgac atcggaaact ctgatttgtt tgcggaagaa aaatcgggga tcgatgcctc
      421 agcattagtc gatcatcgag tagcagttgt tgggatcact cctattgtga acaaggatat
      481 tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta tttacaggcg agatcaaaaa
      541 ctggagtgaa gtcggtggaa aagatcagcc agtagttata ttgaaccgcg cttcaggaag
      601 cggaacccgt agcacgtttg aaaaatgggt attggacgga gaaacagctg ttcaggcaca
      661 agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc gacacacccg gtgcaatcag
      721 ctatgttgct ttttcttatg tttccgatga tgtagatacg ttgaatgtta acggtgtaga
      781 accgacagat gaaaatgtga caacgaacaa atggacgatt tgggcatatg agcatatgta
      841 cacaagagat catccaaaaa aactgacaaa agaattttta gactatatct tatctgatga
      901 agtacaagat aacatcgttg gagaactagg ttatatccct gtttctcata tgaaagtcga
      961 acgtgattgg caaggaaata tcgttaaaga gtaatcttta cacaaaattt acaaacttag
     1021 ctaaactttt acatgttatt aaaaccaaat tgatgttcaa tttgtacact gagtgagtat
     1081 gttaaacctg attaaggggg agtcatctgt ggaagatgtg aaaaaagtat tattaacaaa
     1141 atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc agttttttat gtatcgcgct
     1201 gatcgtgttg gttgtattat ctatttttta ttttgtagct agtaaaggat tggccacctt
     1261 ttttactgat aagatcaatg tatttgattt tctgtttggt acagattgga atccaagcca
     1321 aatcggagca aatgggaaac caatggtagg ggcattgcct atgatcacag gttcgtttat
     1381 tgtgaccttt ttatctgcta tcgttgccac gccatttgcc attggtgcag cagtattcat
     1441 gacggagatc tctcctaaaa acggaacgaa gatcttacag ccggtcatcg agctgcttgt
     1501 aggtattcct tctgtcgttt atggttttat cggtttatca gttatcgtgc cttttgtcag
     1561 aagtattttt ggcgggacag ggtttggtat cttagctggt acatttgtgt tgttcgttat
     1621 gatcttgccg actgtcacaa cgatgactgt tgatgcatta aaggcagtgc cacggcacta
     1681 tcgggaagct tcattagcgc ttggtgccac aagatggcaa acaatctaca aagtcgtgct
     1741 acgagcagcc gtgcccggga ttttaacagc agtcgttttt ggtatggctc gtgcgtttgg
     1801 tgaagcctta gctatccaaa tggtcatcgg taatgcagca ttgatgccaa caggacttac
     1861 tactcctgct tctacattga cgtccatctt aacaatggga atcggaaata caatcatggg
     1921 aacagttgaa aataatgtgt tatggtcatt agcgctgatc ttgctattga tgtcattatt
     1981 ctttaatatc gtgatccgta tgattgggaa gaaaggagag ctgaaataat gaacgcaaaa
     2041 cgctcagata aaattgctac aggtgtttta tatatagttt cggggatcat cgtattgatt
     2101 ttagcagctt tactacttta tatcttagtt cggggaatac cgcatatttc atggtccttc
     2161 ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa tcgggatcca gctatttaac
     2221 tcgatttatt tgcttttgat cacgatgatc atcagttttc ctatttcttt aggtgcgggt
     2281 atctatcttt cagaatatgc tggaaaaaac tggctaacag atgtgattcg cacgtcgatt
     2341 gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat tcggtttctt ggttttcgtt
     2401 attcaattcc agtatggttt ctcgattatt tctggtgcat tggctttgac tgtattcaac
     2461 ttgcctcttt tgactagaac agttgaggag tctttacaag cggtccatta cacacaacga
     2521 gaagcaggat tggccttagg tttgtcacgc tgggaaacag tgataaaagt ggtggttcca
     2581 gaagcgacac cagggatttt gactggtgtg atcctaagtt ctggaagaat ttttggtgaa
     2641 gcagctgcat tgatctatac ggctggacaa agtgcacctg cgttagattt tacgaactgg
     2701 aatccattaa gtgtttcaag cccgatcagc attttccgtc aagctgaaac attagctgtc
     2761 catatttgga agatcaatac agaaggcacg atgcctgatg gtgtttctgt ttcggctggc
     2821 gcttctgccg tgttgatcat tgctgtttta ctgttcaact tcggcgctcg tgcgattggc
     2881 aaacgattat accgaaaaat gacttcagcc taaggagaag aagcaaaatg aaaaaatata
     2941 actggaatga aacaaatgtc atcaccatgc caaaagagaa agaagtagca ctatatacgg
     3001 atgatctaca tgtctggtat ggcgacaatg aagcaatcaa aggagtggat ctggaatttg
     3061 aaaaaaataa gatcactgca ctgattggtc cttccggctg cggaaaatct acgtatcttc
     3121 gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa aatcactggg aaaatcatgt
     3181 atcaaggcgt agatattaat gcgcctcaag tggatgttta tgagatgcgc aaacggattg
     3241 gcatggtttt tcaacgtcct aatccgttca gcaagtcgat ttatgacaat attacatttg
     3301 ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga tgaaatcgtt gaaacaagct
     3361 tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt ggacaaaagt gccttggctt
     3421 tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc gatcgcgatg aaacctgata
     3481 tcttgctgct agacgagccg gcaagtgctt tggaccctat ttcgacagga acggttgaag
     3541 aaacacttgt aaacttgaaa gaagactata cgatcatcat cgtgacgcat aacatgcaac
     3601 aagcagctcg tatcagcgac tatactgcct ttttctatct aggaaaagta atggaatatg
     3661 acaaaactaa caagatattt accagaccaa aaattcaggc aaccgaagat tatgtgtccg
     3721 gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga aattatttca tctaaagatc
     3781 ttcatctgta ttatggaaag aaagaagcac taaaagggat caatctaagt ttcaatcaag
     3841 gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa atccacttat ctgcgttctt
     3901 tgaaccggat gaatgacctg attccgaatg taacgatcac cggaagtgtt ttatataaag
     3961 acaaagacat ctatggtcca aaaacagatg ttgttgaact aagaaaagaa atcggcatgg
     4021 tattccagca gccaaaccca tttccttttt ctatctatga aaatgtcatc tatggattga
     4081 aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt agttgaagaa agcctgaagg
     4141 cagcgtctgt ttgggatgat gtgaaagaca aattgcataa aagtgcttta tctttatccg
     4201 gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc agttaatccg gaaatcatct
     4261 tattggatga accaacaagt gcgcttgatc ctgtttctac tgggaaaatc gaaagtatgc
     4321 tgttagaatt aagagagcgg tatacgatga tcatcgtgac ccataatatg tctcaagctt
     4381 cacgtatttc agataagacg gcattctttt tagatggtca cttgatcgaa tacaacgata
     4441 cgaagaagat attcatgaat ccagaaaaac aagagacaga agattatatt tcaggtcgat
     4501 ttggataagg aggaaacgga atgctacgta cacaatttga agaagaattg ctgaatctcc
     4561 ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc cgtgcacaag tcagttcgtg
     4621 catacatcaa acacgataaa atcttagccc aagaagttat cgaaaacgat ataaatatca
     4681 ataacatgga aacacgcttg gagaagaaaa gttttgaaat gatcgcattg caacagccag
     4741 ttacaactga tttgcgtatg atcattacgg tcatgaaagc tagctcggat ttagaacgta
     4801 tggccgatca tgcggtgtct gttgctaaat ccacgattcg cgtaaaaggg cagacgagaa
     4861 taccggaaat cgaaaaagag atctccgata tgtccgatta tgtgaaaaaa atggtggaca
     4921 acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc acgtacgatc gccaatatgg
     4981 atcaaagagt caatgactat ttcaatcgaa tctataatgc aacaatcaaa aatatgcagg
     5041 aaaatcctga gacagtcatc agtggaacag actacttgaa tgttgccagt tatctagaac
     5101 ggatcggaga ctatgtgaca aacatctgtg aatggatcgt ctacttggct acagggaaaa
     5161 tcactgagtt aaatacgaac cacaatgaga ttttctaaac tgaagctgtg attatgccgt
     5221 ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa aagctgttct gcatcaagta
     5281 ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg acttattacc
     5341 gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa aacatgtcac
     5401 aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt agcatcatca
     5461 ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagct gctttatatg
     5521 aagtacctgt catcgagatc gaaaaagtac gaataaaaga ggatcatcaa ttgattacag
     5581 gaagactgat gaacacggat caaaaaggag aatcgattta tttatcggtt ccttatcaaa
     5641 aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc caaatgcatg
     5701 gagatgaatg gcagctagta gcaaaaaaaa aatgatggct ggcttttctt cttcacaagt
     5761 atctccattg gcgccatgtt gcttattggc ggaaaacagg gtctatttgc tttaggaagt
     5821 ctcatcatca atatcgtttt acttttactt ttgttgctca tctttactaa aacagaaggg
     5881 atttctctat taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg
     5941 gctttagatg gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt
     6001 tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagacca aggactgcgc
     6061 tatgaggaac tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg
     6121 attggaacag ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa
     6181 ttgactaagc gacaaccaga aataacagcg aaacagttat ggctttcagg aaggcaagtg
     6241 ggtcgagatg tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca
     6301 gttccatcgc ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag
     6361 catttatcat tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc
     6421 cctatctcgg tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta
     6481 tacttgttgg gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata
     6541 ttcttttagg cttattcatc aatttaggga tctttttcct gttgttattc ggttatcatc
     6601 agaagtggcc gatactggca ttgtcggtca taggattttt attgattgct gtggttatct
     6661 tattttttat caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct
     6721 ttttattttg tttcctacta ttgcttccta tcactgattt tttagcgatc caaggtttta
     6781 catcggtaga actagaagaa ttatctggct tagataagac tttagcgatt gattttagat
     6841 tacttacgag atcgttgctt ctgattagtc ttagtggtgc tgttttagat gctagtgtag
     6901 caatttctag tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat
     6961 tgcgccattc tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc
     7021 tttttgcatt catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc
     7081 cttttcagca agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta
     7141 ccacattgtc tgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta
     7201 caaaaaagaa agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt
     7261 ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg
     7321 aggcaatttc catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt
     7381 cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa
     7441 taaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat
     7501 tgatcgataa acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa
     7561 gagaaaggca agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga
     7621 ataaaacttc ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac
     7681 gcaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga
     7741 cagaagaaga attagcacag cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta
     7801 gtggcgatac actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta
     7861 agaatcaatg gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt
     7921 ggaaagatca agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat
     7981 cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca
     8041 acatggaatg gaaagatgtc agcttacgtg tccctgggat tgcgacaacg aaatttgagc
     8101 atgaatttta ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa
     8161 acgtgacgtt aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt
     8221 acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta
     8281 atgaagatca tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct
     8341 acttacctaa acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga
     8401 ttgaaacatt ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg
     8461 accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa
     8521 acgggaacat cttagattct attatcgaaa cagtaaatgg tacagtgaca tttggtgcga
     8581 cagcagaaaa cctttctgtg tctttggtaa atggtgatgt acgtcttacg attaaagaag
     8641 ataatttgaa aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag
     8701 atggcatcgg tttggaagga catgctaaaa caagtcttgg aagtatcaac agccgtctgt
     8761 ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc
     8821 gtgtttcaga tggtgaaatc acgcaagtgc aactttcaac gacaacagga agtatctatt
     8881 taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa
     8941 gcgattttgt aaaatagctt cccctatttt acaaaattgt gccttattgc cgataggcta
     9001 tctcttgaac accgtttacc aagagattat gaacctgcca attatcgccg agtcacaagg
     9061 aataatttca gaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt
     9121 tgcagaaaga caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt
     9181 agaacagagg tgagacatga tgaaaaaaaa gttgacaaaa tccaataaaa atgttgtttt
     9241 gacagggact ttagcaggaa taggagaata catcgggatt gatccgactg ttatccgtgt
     9301 gctttatgta ttcctgagct tagttgcttt tggaagtcca atcattttat acattttatt
     9361 agctttgatt atcccatctg cgcgttctgc acgaacaaac tatggacata acaatgcata
     9421 ttatgaaaaa aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc
     9481 ggaaaaaatc gatgatgacg aatggagtga cttttaatgt cgtactttca acgtatttta
     9541 gttaatacgt taacattcat atcattggct gttctttttc ctgcgatggt ccatgtgagc
     9601 agtatttgga cggccgttct tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa
     9661 ccgattttga cgatactgtc cctgcccttt acattagtca cattgggatt gtttacgttt
     9721 gttatcaatg cagcgattct taaaatgacc tccttttttg tgggagcaga acattttagt
     9781 ttttcttcat tcggtgcagc gttgttagta gctatcttga tgtcgcttgt caatgtaatt
     9841 gtcagtgaac ataacagtag aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt
     9901 tttatttaga atcaggaatg cgttttctat tacattggta attaaataaa aacgtttttt
     9961 ttaattggta gtacttttgg ctagacaatg ataaaatgat agagaacact ctaatagaaa
    10021 tgaggtagca tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa aattatcttt
    10081 ggaaatcgtg acaggcgacg agaaaagctt agaccgtgtc atcgtaactg gggacatctc
    10141 acgcccagga ctagagttga ctggttattt taattattat tcccataatc gtttacagct
    10201 atttggcagt aaagaaatca gttttgcgga acggatgatc ccagaagagc gtttgatcat
    10261 catgagaaga ttatgtagtg aagatatgcc cgcatttatt atttcaagag gattaccggc
    10321 tccagaagaa atgatccagg cagcaaatga aaatgggata gctgtactgc gttctcccat
    10381 ctcaacttct cgtttattag gagaattatc cagttattta gatggtcgcc ttgcccctcg
    10441 aacaagtgtc cacggtgttt tagtggatgt ttacggatta ggtgtactga ttcaaggtga
    10501 cagtgggata ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggtc atcgattgat
    10561 cgcagacgat cgagtagatg tttatcagca agatgaatta acggtgatcg gagaaccgcc
    10621 taaaatcctg gaacatctaa ttgaaatccg aggaatcggg atcattgatg taatgaatct
    10681 ttttggtgcg agtgcggtac gtggctctat gcaagtccag ttggctgttt atctggaagc
    10741 atgggcaaaa gataagaaat acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc
    10801 ggaagtgggc attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat
    10861 cattgaagta gccgcgatga atttccgtgc acgtacaatg ggctttgacg caacgaaaac
    10921 atttgaagaa agattaagtc gcttgattga agaaaactca ggcaacgact agcaaggagt
    10981 gacaatatgt tagcacaaat caatcgagtt gcttttgagc ttgtagggat tcctatctac
    11041 tggtatgcat tgatcatcgt atcaggtatc attatcgcca tgtggcttag cagcagagag
    11101 gctgttcggg taggattaaa agcagatgat gtgacagatt ttatgttgtg gggattgcct
    11161 atctccatta ttggtgctcg cttatattat gttttatttg atcttcccca gtatatagct
    11221 gatccaatcc aaatattcaa tatccgttca ggaggcttag ctatttatgg cggactgatt
    11281 gcaggcgcga tcacgctgta tttttttact aaatatcatt ttatttcttt gtggactttt
    11341 ttagatatcg ctgcaccaag tgtattatta gcacaggcaa tcggtcgttg gggaaatttc
    11401 atgaaccacg aagcatatgg accagacacg acgcgaagct tcttagaaag tctgcattta
    11461 ccgcaattca tcattgataa tatgtatatc gaagggatct atcgtcaacc aacatttctt
    11521 tatgaatcac tttggagttt gattggattt attttgcttt tgtttttacg aaagaaacca
    11581 caccttttga aaaaaggcga agtcctttta tgttatctaa tgtggtatag tttcgggcgt
    11641 ttctttattg aaggattacg tacagacagt ctttactttt taggtgtgat ccgtatttct
    11701 caattattat ctgctgtgtt atttgtcggt acattgattc tatttatttg gcgcagaaaa
    11761 aaaggaaatt taccggatta tgaccgctca ttaggaaaaa atcaacaaat tatctgattt
    11821 ttagagcgtt aaaaatgaaa aaaatttcag gagtggtaaa atgaattata gagtagcggt
    11881 tttaggacca ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa acggacatga
    11941 cgttcggatt tggggacatc gtgcttcaca agtagaagaa atcaacaccc aacacacgaa
    12001 tcaacgttat ttaccagatc attatctacc cgattccatc caagcattca ccaatatgga
    12061 agatgcagta aaagacgcag atgcggtgct attcgtcatt ccaacgaaag caatacgttc
    12121 tgttgcaaaa gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa
    12181 aggattagaa cagggaacac ataaaaggat ctctgacatc ttgttagagg aaatcccagc
    12241 agaaaaaaga aaagctgttg ttgttctttc tggaccaagc catgcagaag aagtagcgaa
    12301 acatgacatc actacgatca cagctgcaag tacagatgca aaagcagcaa aatatgtaca
    12361 agagctgttc atgaataatt attttcggat ctataccaat cctgatgtga tcggtgtaga
    12421 gatgggggca gctttgaaaa atattattgc tattggtgct ggcgctattc acggcttagg
    12481 gtttggcgat aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga tcagccgttt
    12541 aggtgtagca atgggtgcca acccgctgac ctttatcgga ttaagcggag taggcgatct
    12601 agtcgttact tgcacaagtg ttcattctag gaactggcgc gcaggcaact tgttaggaca
    12661 agggcagccc ctagaagagg tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac
    12721 tactcaagca gcgatggaac tagcccaaat aatggaaatc gaaatgccca tcactcaaac
    12781 aatctatgaa gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt
    12841 acgtgatggg aaaaaagaaa atgaattctc attaacaatt ttttgaagga gcgaatgtat
    12901 atgaaagtca aaaaagcagt tattccagcg gcaggattag gaacaagatt tttaccagca
    12961 acaaaagcta tggcaaagga aatgttgcca attgttgata aaccaacaat ccagttcatc
    13021 gttgaagaag cgttgaaatc aggaatcgaa gacatcttga tcgttacagg aaaagctaaa
    13081 cgaccaatcg aagatcactt tgattcaaat attgaattag aaacaaacct aagtgaaaaa
    13141 ggaaagaccg aattattgaa attagtagaa gaaacgacag atgtcaactt gcatttcatc
    13201 cgtcaatctc atccaaaggg tttaggacat gccgttcttc aagcaagagc ctttattgga
    13261 aatgaaccgt ttgtcgttat gctaggcgat gatatcatgg aagatgaagt accattgaca
    13321 cagcaattaa tcgatgatta cgaacgtact catgcttcta cgatcgccgt tatgcaagtt
    13381 cctcatgatg aaacttcaaa atacggaatt atcaatccag gagaagtatt ggaagatggg
    13441 ttatacaatg tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta
    13501 gctattattg gacgttactt attgactcca gaaatttttg atgtattgtc cgagcaacaa
    13561 ccaggagccg gaaatgaaat ccaactaact gacgcaatcg atacattgaa taaaacacaa
    13621 cgcgtatttg cccgtaaatt cacaggtaaa cgttacgatg tcggtgacaa atttggtttt
    13681 atgaaaacaa gtattgaata tggtctaaca catccagaag taggtgcacc tttacgtgag
    13741 tatatcctgt ctttaggtaa aaaattagaa gcagaaggaa gcaagatcga tcataatcca
    13801 aaaggaataa cagaaatcaa agaagaaaag aaaaaataac ccttttttct aacgagcttc
    13861 agctgctaat tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa
    13921 atgctatcct tatagaaaag agagacaaga agggagtttg atcatgtcag gaggagaaat
    13981 tgcaggattg atcgcagcaa tcgcctttgc gatacttgtc gtttttatcg ttcgtgtgtt
    14041 gttgcaagtt tctaaaactt tagaaaaagt agatcagact gtagcagaag caaatacgac
    14101 gatcgaaatc gtcacaaaag acgtagatct tttgtctaga caagtagaag gcttgctggt
    14161 caaaagtaat gaactgctct tggatatcaa tggaaaagtc gcaacgatcg atccattgtt
    14221 cacagcagtt gctgatttaa gtgaaagtgt atcagaactt aatcattcta gtaaaaacat
    14281 cgcgacgaaa gtcggcggaa tcggtaaaac gacagctaaa gcgacggttg ccggaaaagt
    14341 cggtgggaca gcgatgaaat tttttaaaaa caaaaaacat actgaaacga caggaggaaa
    14401 ataaaaatgg ctaaaaaagg tggattttta ttaggtgcag tagtcggagg aacagccgca
    14461 gcaatcgccg cgttattgtt agcaccaaaa tctggtaaag aacttcggga tgaattagct
    14521 gcccaagcgg atgatttgat ggatatggct tcagattaca cagatatggc gaaagaaaaa
    14581 tcgactgagt taacaggtat tgcaaaagaa aaagcgcaag atctttctca acaagcaggt
    14641 gatctagcag gtagtgtcaa acaaaaagta agtgaatcag cagatgaagc aggaagtgaa
    14701 tctgacgata tcttgttaaa tcttaaagaa caatcagctg acttatctaa tcgtttcaaa
    14761 aaaacggcag aagatatgaa aaaacctgca gatgaattag gaagtattgc taaagatact
    14821 tcagaagata tcatgattga tgtaaaagac gctgctggta aagcaggaga tactgtcaat
    14881 gaaggaatgt cagaagcaaa accagttgca gatgacacaa aagaaatgat gaaaatggca
    14941 aaagaagatg cgaaagaagc taaaaaagaa ataaaagata caatgacaga ttcatctaca
    15001 aatcactaaa atcactaaaa tcacaaattt atagaataaa aaggtgggag cggactagct
    15061 ctcacctttt ttactcttat ttttctaaaa ctagtaattc ttttggtgta gtcgtgaatg
    15121 gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct actttttcag
    15181 gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact aagtcatttc
    15241 cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct aaacggtgat
    15301 tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga tcaagttcac
    15361 ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca agagtgatgt
    15421 tgtatatttt ttcttggaag tcggttggtt tttgataagc aattgtacga gtagcatcac
    15481 tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc ggtgaaatct
    15541 tcgtgtttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg actaatgtgt
    15601 cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcgatttct gcaatgatcg
    15661 cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca acatcggccc
    15721 atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt tgtagacgtt
    15781 ggatgatgga agctagatca gtgtaagaat ccgttcccgt caagatatca gagatagcat
    15841 cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc tgaccatagc
    15901 gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa tagccacgga
    15961 catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga gtgaaaagga
    16021 aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga tcactttcgt
    16081 atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg acttggtgtt
    16141 gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa cctactttct
    16201 aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa cgttctaaaa
    16261 actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat tcttgagatt
    16321 atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata aaatatgaat
    16381 aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag agaggcaaat
    16441 gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgtcaa accagcaaca
    16501 agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc agttgcgcgt
    16561 ggcttggcaa gtaagaaaac aacaacagtt ggggtaatca tccctgatgt cagcaatatg
    16621 ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa atacaatatt
    16681 atcttagcaa attcagatgg taacgaccaa aaagaagtca atgtattgaa taacttactg
    16741 gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga aatccgtgga
    16801 gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc agatgaacaa
    16861 gtaggtagcg taaacattga ttatgtatca gcaacaaaag atgcagtaaa caaattggca
    16921 aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc tatcaatgga
    16981 caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc atatagcgaa
    17041 ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt cgatcggatc
    17101 cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat cggtattttg
    17161 gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat cacaagtaac
    17221 aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca accattatac
    17281 gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga agaagtcgaa
    17341 gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa ataataaaag
    17401 gacaaccatt cagaggttgt gagcctgccg tttaactctg cgttacaagg agcaatttca
    17461 aaaaacagca tttcatgttt gatgaatatt gtgactcgtg attcggagtt ggcaggagaa
    17521 caccgtttat tcgggaaaag gggttatgac aaaattttgt ttaatccttt ttttttgtct
    17581 taaaaaagag agaaagtggt tgaaattaca tttatcgtat ataaaacaat ccgtgtatag
    17641 atgctgcatt atgttcacca ccatcaaaaa aataagggac tgtgacaata ctattgtcac
    17701 agtcccttat tactgtgtat tctattaaat caatcatctt tctcatctga ttctttggtt
    17761 gcttgattcg ctcttgcata agctgaagcg gtgttccagt aatctgtata gttttcatcg
    17821 gtaccgccaa cgccaaattt aaaggcgctt ttcttcggtc catttttcgc ccatagcgat
    17881 tcaactgttt ttcctggtgt ttggatggat tttttattga cagtaacttt tccaggtgtt
    17941 tgaccagtaa atccagatac ttttttcttc atcacatcag atgataattc gaatttctca
    18001 tcagtgccaa aaatgtcagg ttttgcttgg taagcttgat tgatcagatt ggcaagataa
    18061 ttagccgaac gtatccttgc tttgctgtcc attccagtat tgtcatcgtg ccctgcccag
    18121 ctactgatgg taatagacgg agtagagaca attagccatg aatctcggta ttcattggta
    18181 gaaccagttt ttccgaccca atctgctttt cctagatttc cgtttaagct tgagatggca
    18241 tctttgaatg gtgtagtgat ttttgcattg atcacactgc gcatcatgtc attcatgatc
    18301 gatgccgttg cttccgaata gatccggaca gagttgcttt cgtgtttata aattacattt
    18361 cctgcgttat ctgtgatgga atcgatgata tagccttctt catagacgcc gccgtttgct
    18421 aaagcctgaa atccattggt ctgttgaagc gtcgtgacat ctactgtacc taaaggagca
    18481 gattcaacgc cccaattgtc atttgccggg tagttcatct ttgccaagta gtgctcgtaa
    18541 gcatattgtt tcgatccacc ggaattcaac acatcttgat ataagtgata agccgggata
    18601 ttgttggacc attctagtga ctcacggacg gtttggaagg tgttcgatcc ttcgttagta
    18661 gcattgacga ttttctcgcc agcattttct ccttcttgcc aagttgttgc ataatctgat
    18721 acacgggatt ctgatccgat aagccccatg tcgattgcag gtccataaac aagaactggt
    18781 ttgattgatg agccagcttg acgttctgta tcgaaggcat ggttattttg attctgactg
    18841 taattccgtc cgccaacgaa tccgtagatg cggccagttc tgttatccat caatacattt
    18901 ccggtttcta cttgactgcc agcaccatca tctaacatgt atccataatt ttctacgcca
    18961 ttttgcaatg cagcatagac atctttatcg attgtcgaat ggattgtata tcctttattt
    19021 tggatcgttt gctgtgcctt ttgataataa gcgtcactga ccgatgaatc agacatatca
    19081 gcgttgtctt tttcagccaa ttgttgtgca atgattcttg ttgcttcatt catcacagta
    19141 taatacaaga attctctgtc agtctgttct gcgatttgtt gtggtagaaa gtcttttgta
    19201 aggtcatagg cttttgcttc ttcgtattgt tctttcgtaa tttggccttc acgatacata
    19261 ctgaataaaa caaaatcttt tctggcaagg ccggcagaaa gatcttcttt tagcgcacct
    19321 gtgttcgtgt atggactata cgtgatcggg ctttggggta atccagcgat ataagcagct
    19381 tgcggcagcg tcacatcttt tgcatctaca ccaaagattc ctcttgcagc ttcctggact
    19441 ccagcaatgt tttgtccctt attattacga ccaaatggtg agacattcaa atacgtagca
    19501 atgatctcat cttttgaaaa gtatttttct acttgtgcag aaagaatgat ctcgtttgct
    19561 ttacgtttga atgttgtttc atctgtaagg atctgctgtt tgactaattg ctgtgtcaaa
    19621 gttgatccac cagaagagcc aataccagta gcttcagata ctaatgctcg taaaacagct
    19681 ttaggaacgt aaccttggtg tttatcgaag tattcatcct ctgtgctgat aattgcagtt
    19741 ttcaataaag gtgaaatttt atcagatgaa atagtcgtgc gcatcagatc tgtctggatt
    19801 tttgatatct ctgtattatc tgcataaaca atctttgacg tttctgtaat gtttcctagt
    19861 tctgtctgca gtgctttttt agtcggcaat tgtgtgtctt cgacgagata ggcaaaatag
    19921 ccagcgccta ttccaatacc tagagctgcg aacatcaaaa gaagaacagt aataaaaaca
    19981 gtcaatgatt gaaaaactct aaaaataatc ttaggaacaa accatttttc tttttttctt
    20041 ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac taatggtttt
    20101 atttcgctaa ttatagcaaa taatagaaca aaaaaacaga caaggctgtt tttggcagga
    20161 atttcttttt taaaagagtt aaaacgtgag aaaatgatct tttttataaa agagttctta
    20221 agcttttctt tagagaaaat ttcagttact atggagaaca gggcaaagaa aaatcaatca
    20281 ttttctttgt atatcatctt accaatatat aggaattgtt agtaacaatt gcctttcaag
    20341 gaggaatcca tccatgttat taggctcaca cgtcagcatg agcggaaaga aaatgctgtt
    20401 aggagcagca gaagaagcag ccagttatgg atcttcgact tttatgatct atacaggcgc
    20461 ccctcaaaac actagaagaa aagccatcga agagatgaat attgatgccg gaaaagcgta
    20521 catggcagaa aacggtctaa caaatatcgt cgttcatgcc ccttatatca ttaatttggg
    20581 gaatacgatc aaaccagaaa tgtttccttt tgccgtccaa tttctgagag aagagattgt
    20641 tcgtgcggaa gctttaggcg ctactcaaat tacacttcac ccaggtgctc atgtaggggc
    20701 aggaacagaa gccggaattg ctagaattat tgaaggcttg aatgaagttt tgacaaaaga
    20761 acagacggca caaatcgctt tggagacaat ggcaggtaaa ggtacggaaa tcggtcgaac
    20821 atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg aatgaaaaat tatccgttac
    20881 ttttgacacc tgtcatacga atgatgccgg ttataatatt aaagaagact tttcaggtgt
    20941 aatggaagag tttgataaga tcatcgggtt agatcggctt aaagtactcc atatcaatga
    21001 ttctaaaaat ccgcaaggtt cgcataaaga ccggcacgcc aatattggtt ttggaacaat
    21061 cggattcgac acgctgaata agatcgtcca tgatccaatg tttgacaata tttcgaaaat
    21121 cttggaaact ccctatgtcg gcaaagataa aaagaaagct tacgcaccat atggaaaaga
    21181 aatcgccatg ttcaaagcac aaatattcga tccagaagtg tttcctgagt tattagcaga
    21241 agaagtgata ctctaagatt ttctatctga cagcagcaaa aaaggaccag gcacattgcc
    21301 ttgtcctttt ttgtttaaga aacaaaggag tgctttttgc agtctttttc atcatgcatc
    21361 ttctcccccc tctttttaaa tttagacaaa cagggtatac tggtagaaaa gtaaaaaaac
    21421 gaaaagtttc acaagcaagt ggcaaaacgt gattggaagg acgaaaagat gctgaacact
    21481 ttaaaaccct tgatcaacca actaaaaaag aaacatacat actttattct tggcatagcc
    21541 ctattattta caattattcg tttgtattta tatagtaaag caatctatgg aacagacatg
    21601 tcggcaacct atgatgacca gctgctgatt cgctatgctc aaaatatttt ggacggtaaa
    21661 tggctagggg agtatacgac tacaacactt tccaaaggaa tctcctacag cttattttta
    21721 gtacttgcac ataaactgca tctttcttat gcagttttgt tcggcatgtt cagtattttt
    21781 gccagtatat tggttgcact cgcgtttaaa ccgatcgtga agaacaagta tcttctttta
    21841 agtttctatt tttatttttt gttttctccg attagtttta cacatgaata ttccactcga
    21901 acgtatcgta atgccctcgt gattccggct gttgagatca ttatcgcttg tttactagct
    21961 atttattacc gtaaatatag ttccagcaaa aaaatgctgc cgtggttttt agggctagct
    22021 gtttcttttc ctttcttttg gttcattcgc gaagattcca tctggcttct tccttttact
    22081 gtagtcgcgt taggcatcag tttcgtccag atcttgctag gacaagactt ttctttcaaa
    22141 gaagcaaaga agttaaaaga acgaccattt ctcctttcga aacaagaatg gctgaaactt
    22201 tcattgtttt tactccctct agtcggtttc tccatcgtct atcagtggat cgaatataaa
    22261 aacgagacaa catatagtat tgccgccatc aatgaccgtt cgagtggcga atttggacgt
    22321 ttgatgaaac agctgatccg aatagatgac gaaacgaact taaataaaga aaacagtgag
    22381 atatgggtct cgcatgctgc attaaataaa gcattagacg catctcctac atttcatgct
    22441 gtttctactc gtattgacga attgtatacg actcatgcgt ggacccaagg tggaaaagta
    22501 aaagaattac caggagatat cattttttgg gcattgcgag atgtggtaag cgaatctggt
    22561 tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa tcaagaatta
    22621 gaagaagcct atcaaaaggg aactatcaaa aagaaaaaag aattctatct gatgggatca
    22681 ggggatggaa agacgatgaa tgatctgcca atcgtatttg cttttctgaa atcaggatat
    22741 gcctatgcat ggcgatacaa agatttccat caagggggga attatagttt cggtacgcaa
    22801 gaacaagtcg aacaggccca agaattattg aatcttccac taatggataa ttggatcgat
    22861 agcagtgatc cgagaacacc tgatttccag ttgacaaaaa cagcaaaagt cgcgaatgtc
    22921 atgataaaaa tttaccaagt tctggccatt cccgtgctga ttgcttcaat agcagggata
    22981 ttacttatga tactaggaac cgcatttgca aaagctgatc gtgcctatta tagttcctct
    23041 ttgattgtag tcaccggatt aatactgaca caattggttt ttcttttcgg cgtctcgtgg
    23101 ttttgttctt atgcgccaga aaaaaaggat tattttttaa gtgtctacac tggcgcaggc
    23161 gtgccattaa cgcaattcat cgtagtaaca agcatgctag ttttcagtaa attgaatctg
    23221 aaaaaagctt tatcttccaa ataaaaacat actaagatta gaggagaaca tcattgacga
    23281 atgatgttct cctctatttt ctatttttag attatagtga agagtgaaag aaagttcgaa
    23341 caaagccttg aggacta
//
LOCUS       DAZKOR010000044        23246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000044 DAZKOR010000000
VERSION     DAZKOR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.044"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACG8ZM_001583"
     tRNA            2..74
                     /locus_tag="ACG8ZM_001583"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACG8ZM_001584"
     tRNA            78..150
                     /locus_tag="ACG8ZM_001584"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACG8ZM_001585"
     tRNA            155..225
                     /locus_tag="ACG8ZM_001585"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACG8ZM_001586"
     tRNA            238..311
                     /locus_tag="ACG8ZM_001586"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACG8ZM_001587"
     tRNA            323..394
                     /locus_tag="ACG8ZM_001587"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACG8ZM_001588"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACG8ZM_001588"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0492548.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACG8ZM_001589"
     CDS             1983..3455
                     /locus_tag="ACG8ZM_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0492549.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACG8ZM_001590"
     CDS             3538..4326
                     /locus_tag="ACG8ZM_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0492550.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACG8ZM_001591"
     CDS             complement(4393..5061)
                     /locus_tag="ACG8ZM_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0492551.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACG8ZM_001592"
     CDS             5176..6888
                     /locus_tag="ACG8ZM_001592"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492552.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="ACG8ZM_001593"
     CDS             6891..8660
                     /locus_tag="ACG8ZM_001593"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492553.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="ACG8ZM_001594"
     CDS             complement(8865..9431)
                     /locus_tag="ACG8ZM_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0492554.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="ACG8ZM_001595"
     CDS             complement(9431..10387)
                     /locus_tag="ACG8ZM_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0492555.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="ACG8ZM_001596"
     CDS             complement(10387..11043)
                     /locus_tag="ACG8ZM_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0492556.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="ACG8ZM_001597"
     CDS             11413..13431
                     /locus_tag="ACG8ZM_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0492557.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13501..13707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13858..16272
                     /gene="leuS"
                     /locus_tag="ACG8ZM_001598"
     CDS             13858..16272
                     /gene="leuS"
                     /locus_tag="ACG8ZM_001598"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0492558.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16620..18173
                     /locus_tag="ACG8ZM_001599"
     CDS             16620..18173
                     /locus_tag="ACG8ZM_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0492559.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18554..20338)
                     /locus_tag="ACG8ZM_001600"
     CDS             complement(18554..20338)
                     /locus_tag="ACG8ZM_001600"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492560.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20353..22074)
                     /locus_tag="ACG8ZM_001601"
     CDS             complement(20353..22074)
                     /locus_tag="ACG8ZM_001601"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492561.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22174..22935
                     /locus_tag="ACG8ZM_001602"
     CDS             22174..22935
                     /locus_tag="ACG8ZM_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0492562.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7332 a         4180 c         4843 g         6891 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtatctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag tagccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaac
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt
    13801 tttttcgtta tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg
    13861 agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact
    13921 ttcaacaccc atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat
    13981 ccatccggtc aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt
    14041 tcacgtgtga aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc
    14101 ggtctgcctg ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa
    14161 aaaaatatcg aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat
    14221 cgcgaagtga atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag
    14281 ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta
    14341 ggaacagtta tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat
    14401 gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta
    14461 ttggatgatc tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg
    14521 atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat
    14581 accgtattta cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct
    14641 gaactagatc ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat
    14701 atcgaagaga cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca
    14761 ggagtattta ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg
    14821 atcgccgatt atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat
    14881 gatgaacgtg actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa
    14941 ggtggagatg ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt
    15001 ctagacggca tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat
    15061 ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt
    15121 tactggggtg aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca
    15181 gaagaagagc taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga
    15241 gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa
    15301 aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg
    15361 cgttatatcg accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg
    15421 ctaccagttg acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca
    15481 cgattctggc ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa
    15541 aaactataca atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt
    15601 ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg
    15661 tacgaaatgt tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa
    15721 ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg
    15781 cgtgaccgta tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg
    15841 aaaaaagtga cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg
    15901 gtctttatca atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt
    15961 gttcaactac tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga
    16021 aatgatggtg gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa
    16081 gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc
    16141 cgagacttag gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa
    16201 tatattgaag gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc
    16261 gttgctaatt aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
    16321 ggccagaatg aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt
    16381 gatcaaaaag gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat
    16441 ggtcttgtaa gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg
    16501 atgtataaac tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg
    16561 actagtgatt gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa
    16621 tggatcaaga actatttatt atcgaagtat tatcagctaa acaaaaaatt atcgagaaag
    16681 cgaaaaaaag agcgaaaaga ataatgttca agcttttaag cttagtattt ttctctggtt
    16741 tgcttttttt cggagctgta tcgttcctat atcgccatat gagtgcagta gtccgttcag
    16801 ctgaagggat gcaaaaatta aataatttgt atcacttttc gtggggagtt ggcggaataa
    16861 caattgttgt gcttattgtt tttcttctac ttatttggct ccgttaccat caattgtcgc
    16921 gtcatttcga tcaatggctt agtcaaagag cacaacagtt tttacataat aaaccaactt
    16981 ctcaagatga tgactatctt tattttgaga agacttcgac gagtcaagct tttcggatac
    17041 aaaaaagtga ttgccagtta ttgctgagat cacttgatga tttacctatc tacttaggcg
    17101 aaagcaaatc aaaattcgga atgaacacat tccatatttt tttactagaa aacgaaccca
    17161 gatgggaaaa aagccagtct tcgcctggtg gatacctgac aactaagatg agtacattag
    17221 tcactggttt tttcctcatt cttttagctg gaacaatcta ttatgggaga tacactcagc
    17281 caaaaatgtc ttctcatata aatgctgctt ctcacatgac aatgaactca actaacgata
    17341 cgcatataca aaagacaaat gaagggttgt tagctgatcc taagcaaaag gtaaatttga
    17401 caacacaaaa gataaatgat ctcaaacttg atacagagac gcactctctc tatatgacaa
    17461 cagatagtgg tgctaattgg gtatttgtgc cgattgattc gcaatggttg cgttttggcg
    17521 aatacaccct gacaacgggg ctgattcctg aaggttattg gatggacaac acatatgata
    17581 tttccgccga tttctcatgg tttatctatt cttctgggga tgatgtacgt ctgctgactt
    17641 caagagataa tggtaaaaca tggcaaacgt ctattatcac ccaatcagtt cagccgatac
    17701 gttatcgaaa agcggtattt tatggcaacg atggtctatt tatctgtaca tcagccagtg
    17761 ggatgtctgc agaaactttg tacatttaca ctactatgga tggcggaatc acttggataa
    17821 aaagtggaag tacaatgatc aatcagccag tacaaaatgt cagcttcgtt acaacttctt
    17881 taggttttgt ttcgacacgt gaaagtttgt tttatacaaa caacggagga cgctcattca
    17941 aagaatcagt tctgtcgatt ccggaaaact atcagacagg ggggatagat ctttttcaat
    18001 caccaaatga agttactcaa ataagcgcga atacgttaga gacaaaattt tatttgttga
    18061 aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct cttccgttca actgataacg
    18121 gggaaacatg gcaattcgtt cagcaactat cgcaagtaga acaaggagat taaagaagag
    18181 agattgtgag cctgacgttc agcaccgagt cacaagaaac aaattcaaaa aacagcttct
    18241 catatttgtt gaatagtgta actcgtgatt cggagttgac aagagcaaca agaagcgatt
    18301 ttgaagtata gcttctcgta ttttacaaga tcgtgtctta ttgccgatag gttgtctctt
    18361 gaacaccgtt tattcgtaaa aagggattgt gacaagtctt ttgtcactac ttaccgaacg
    18421 aaactgaagg agctatccaa aaagtatcat tagtattatt ctttgtgttt ctagtggcac
    18481 cactagtaca tttaaaaaag ctatcgatca tggactaatc atacatgatc gatagctttt
    18541 tagataatgt ttgttacgct atctgaaatt gactattata aagactggca taaagcgttg
    18601 gtgattggag tagttcagaa tgtgttcctt tttcgacgat atcgccattc ttcattacaa
    18661 ggatcatgtc tgcattttca atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat
    18721 cttttgtcac agcttccatt gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag
    18781 aagttgcttc atctaaaata acgacaggag gattcgctag aatgactcga gcaattgtca
    18841 ataattgttg ctgtccttga gataacgtgc cgttttcgct agaaatgatc gtatcatatc
    18901 cgtctggcag tgttcgaata aagtgatcac attgtgcgat ttcagccgct tcgataattt
    18961 gttctcttgt ggcatccatt ttcccgtagg cgatattatc agctaccgtt ccttcaaaca
    19021 gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag ttctccacgt gaaagcttgg
    19081 taatatcaag accgtcgaat tcgatctttc ctttattcag ttcataaaaa cgcatcagca
    19141 gattgactaa agtcgttttt cctgcaccag ttggaccaac gatcgctacc gtttgttttg
    19201 gctttactga aaaatcgact tgattcatca atattttatc tgatgtgtaa ccaaactgta
    19261 catttttgaa agctattttt cctttcggtt gttctaaaag tcttgcatct ggaaggtctg
    19321 gaatttcatc tgtttcatct aatatctcaa atatcctatc gattgctgcc attgcggatt
    19381 ggatcgagtt gatcacataa gaagcggtag aaattggttc agaaatctga ttgatgtact
    19441 gaagataagc ttgtaagaag ccgatcgtca caccacctga tagaacaagc atagcaccga
    19501 ataccgcact tatgatgaag gcgagttgat taatgaaacg aatggccgga tagatagcac
    19561 agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt tttatttact tcgtgtatag
    19621 cagcttctgc atgagcttgc tgattgaatg ttttgaccac tagattacca gctaagattt
    19681 cttccattcg attatttaaa acacccagtt catactgatt ctgttcagaa atctgtttgt
    19741 ttttgttcgc aatcttagtt gtaacaattg aactgccaac gatcagcaat aagacgagca
    19801 aagtaagctt gctttcgata taaaacagca tcagaccagc agcagctatt gttacgacag
    19861 aagtaaaaaa ttgattgata cctgtcaata acacttgaga aagttgattg agaccggtag
    19921 ttgtccgact aatgatgtca cctacttgat gattgtcaaa aaacgacatc ggtaatgttt
    19981 tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca
    20041 ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac tgaacagccg atcagcaata
    20101 agacagggaa caacaatgct tgtttgatat cactgaaaga aagctgtcca atacctgttt
    20161 ttcttatttc tgataagaga tcatcgatcc cgattcccat gatcaagggc atggctacga
    20221 ctaataggtt cccgaataaa ctgcatagaa acaatccgta aaaatattta tgttcaggtt
    20281 tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg
    20341 tatcccttct ttctatattc cttgtgattt tgcaaactcc tgataagttt ggcaattaga
    20401 cagtagttct tcatgagtac cttgtccaac gatttttcct tcatctaaaa caatgatgtt
    20461 atctgcttgt tggattgtac tgaggcgttg agcaacaatc acgagagttt tctccgacat
    20521 gtgtttatga agggctgctc ttaatgctgc atctgtttta tagtctaaag cggagaaact
    20581 atcatcgaat acatagatct ctgctggttt gatcaacgct cgagcaatac atatccgctg
    20641 tttctggccc cctgaataat tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg
    20701 aagggtgtcc actagttctt ctaattgagc gatcttgatc gctttagaca actcttctgt
    20761 tgaggcatat tcatttccca tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc
    20821 cttctgaggg acataactga tgtgggagcg gatctgttct tgagtcattt gagtaagagg
    20881 aataccacca agggttactt ttcctatcgt agggtcacta agtctcaaga gaagtttcgc
    20941 caaactactt tttccagatc cggtcccgcc aacgatcgct gttgtttttc ctttaggtat
    21001 tgtgaaatca atatcttcta gaactggatc agagatttca gtatatttaa atgtgacatg
    21061 ttcaaaagca gctgcatttg tctgatcaaa ggttggttca gctactgttt tgatcgtctc
    21121 ttcctcaatt tcgatctctg cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga
    21181 tttagggata atgaccaata ccatagctgc gatcatcaag taagcaagtg tcagcataga
    21241 gtattcgact acagcagtca cgacaccgat ttgtaatgct ccttctgcta caagatttcc
    21301 gccaaaccac aaaatagaag cataaacgat tcccatgagc aaaaaagcaa ttggagtgac
    21361 ccaagcaaat attcggttca ctttgatcat ttgattagca tagtcagcaa agctcttatt
    21421 cgttcgcttt tcttcatatg attgattatc aaaggcacgg atcatattaa tcccagtaaa
    21481 gaattctctc aatgtgatgg tgatccgatc catttttggc tggatactca aagataaagg
    21541 tgcccctcgt ttcatcagtc gataaacaat ccatacataa aaaacaacag agatcaatgt
    21601 aacccatgtt agtaatagag aataacgaaa catcatgaga atggtgaaaa cgcaaataac
    21661 cggtgctggt aagatcattt ggcaaaataa aacaatcatt tgctggacat tatcgacatc
    21721 attcgtcatt cttgtcaaaa gagatccaga actaaaacga ctgatatcct tcattgacag
    21781 ctgttggaat ttacggaaat atttctcccg cgtttggaat ccaaacctag cggaagccaa
    21841 tgctgaaaag taactagagg caactgcagc gattgttcct agaaacgcta caagcagcat
    21901 ttcaactcca atttttttta taactgaggc ttcgccactt gctatacctg tgtcgatcag
    21961 ctttgctact aattttggta cacctaatgt accggcgatt tctaaacaga tcatcacaaa
    22021 ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt tttaagatag acatattatt
    22081 cctccttttt tcctgatact tttcgattct ataccttcaa gtaacttgaa ggtcaataga
    22141 aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa
    22201 gtatcggaac tatttgattt gccaaaatca acgatccgct attgggatga acaaggatta
    22261 atccgttcaa gtagacaaga agagaatgat tatcgattgt ttgatatcga tgatattttt
    22321 atgctttatg atatcgtttt ttatagaaaa ttagatatac cgatcaaaca gatgaagaat
    22381 ttatatggaa aaacattgac ggagttatat gagacacttg atgaaacaga aagaagaatc
    22441 cataaagaac tggtggtgat gaaacaaaaa caaaaagaga taagggagca aaaaaaacag
    22501 ttgaaattga tgatcgatac aaacgaggaa gaatttcctg tagaagagat cccttttgac
    22561 tgtatgatct caacggagtt tgaagatatt gtggaaatca aaaaatttct acctaactat
    22621 tcaagctttg ggatgatgag tatgccagct tccagttcac agacgatgta tggatttttt
    22681 atcgacccta gtgaagtaca tttatttact caagacgtga tttgggagaa aaaagatacg
    22741 gctgtttatc gtcgattctt attgaaatcc gagatgaacc atgcggaacg taataatatt
    22801 ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa caggagaagt gatcggccag
    22861 tatctattga cgaatacgga tgaaaacaat atcagaactg aatattatca tgcatggata
    22921 gaaatgaaaa aataggaaat atgtgtttaa gataactcga aaatgatggt tgttttagtt
    22981 ataaagaaaa attttctggt tcgattacgg gtttaatgtg ggggattatt agtcgtggat
    23041 agatagaatt ttagtttttt agaggaaata tgtctttaaa aatggaatgg ttgcaattct
    23101 tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg
    23161 tgttatgtat aattcatggt gaactgagac taatgagaag aaaaattcat attgttatga
    23221 gtgagattgt atcagatgag aatgat
//
LOCUS       DAZKOR010000045        22763 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000045 DAZKOR010000000
VERSION     DAZKOR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.045"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8ZM_001603"
     CDS             6..305
                     /locus_tag="ACG8ZM_001603"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0492563.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8ZM_001604"
     CDS             378..1739
                     /locus_tag="ACG8ZM_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0492564.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8ZM_001605"
     CDS             1761..2021
                     /locus_tag="ACG8ZM_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492565.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8ZM_001606"
     CDS             2035..2787
                     /locus_tag="ACG8ZM_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0492566.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8ZM_001607"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8ZM_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0492567.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8ZM_001608"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8ZM_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0492568.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8ZM_001609"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8ZM_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0492569.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8ZM_001610"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8ZM_001610"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0492570.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8ZM_001611"
     CDS             7161..8558
                     /locus_tag="ACG8ZM_001611"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0492571.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8ZM_001612"
     CDS             8639..9079
                     /locus_tag="ACG8ZM_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0492572.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8ZM_001613"
     CDS             9227..9553
                     /locus_tag="ACG8ZM_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0492573.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8ZM_001614"
     CDS             9554..10303
                     /locus_tag="ACG8ZM_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0492574.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG8ZM_001615"
     CDS             complement(10383..10838)
                     /locus_tag="ACG8ZM_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0492575.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACG8ZM_001616"
     CDS             complement(11265..11540)
                     /locus_tag="ACG8ZM_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492576.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8ZM_001617"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ACG8ZM_001617"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0492577.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8ZM_001618"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ACG8ZM_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0492578.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ACG8ZM_001619"
     CDS             14770..17436
                     /locus_tag="ACG8ZM_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0492579.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ACG8ZM_001620"
     CDS             17800..18552
                     /locus_tag="ACG8ZM_001620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0492580.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ACG8ZM_001621"
     CDS             complement(18574..19728)
                     /locus_tag="ACG8ZM_001621"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0492581.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="ACG8ZM_001622"
     CDS             19913..22354
                     /locus_tag="ACG8ZM_001622"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0492582.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22511..>22763)
                     /locus_tag="ACG8ZM_001623"
     CDS             complement(22511..>22763)
                     /locus_tag="ACG8ZM_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0492583.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         7290 a         3995 c         4750 g         6728 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttggctct tcgtcaaatt
    22441 gtgttggcga agacaaattt gaataaaact tagtccgata tgcggcgcgt tctgtgcgcc
    22501 gcatattttt ttagttttca aagatcagac cttgttgtaa atagatccgt gcccgaaagt
    22561 tataaaagtt gcgataacca aacgcaattc gtttaatcag cttgatcttg ttattcagcc
    22621 cctcagtgac gccgtttgag tggggcacct taaaggcatt tgaaataccc gattggtagc
    22681 gatggaagat cgtgaatttc tttttaaacg actgaggtaa cgtagatgaa attgttttaa
    22741 gggtttcttg aaagccttcc gta
//
LOCUS       DAZKOR010000046        22736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000046 DAZKOR010000000
VERSION     DAZKOR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.046"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..1959)
                     /locus_tag="ACG8ZM_001624"
     CDS             complement(28..1959)
                     /locus_tag="ACG8ZM_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0492584.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1977..2891)
                     /gene="pfkB"
                     /locus_tag="ACG8ZM_001625"
     CDS             complement(1977..2891)
                     /gene="pfkB"
                     /locus_tag="ACG8ZM_001625"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0492585.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2888..3640)
                     /locus_tag="ACG8ZM_001626"
     CDS             complement(2888..3640)
                     /locus_tag="ACG8ZM_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0492586.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3800..4009)
                     /locus_tag="ACG8ZM_001627"
     CDS             complement(3800..4009)
                     /locus_tag="ACG8ZM_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492587.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4020..5347)
                     /locus_tag="ACG8ZM_001628"
                     /pseudo
     CDS             complement(4020..5347)
                     /locus_tag="ACG8ZM_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5483..5638
                     /locus_tag="ACG8ZM_001629"
     CDS             5483..5638
                     /locus_tag="ACG8ZM_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0492588.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5712..6299)
                     /gene="yihA"
                     /locus_tag="ACG8ZM_001630"
     CDS             complement(5712..6299)
                     /gene="yihA"
                     /locus_tag="ACG8ZM_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0492589.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6382..7632)
                     /gene="clpX"
                     /locus_tag="ACG8ZM_001631"
     CDS             complement(6382..7632)
                     /gene="clpX"
                     /locus_tag="ACG8ZM_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0492590.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7879..9168)
                     /gene="tig"
                     /locus_tag="ACG8ZM_001632"
     CDS             complement(7879..9168)
                     /gene="tig"
                     /locus_tag="ACG8ZM_001632"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0492591.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9322..10254)
                     /locus_tag="ACG8ZM_001633"
     CDS             complement(9322..10254)
                     /locus_tag="ACG8ZM_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492592.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10411..11070)
                     /locus_tag="ACG8ZM_001634"
     CDS             complement(10411..11070)
                     /locus_tag="ACG8ZM_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0492593.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11073..12098)
                     /gene="sppA"
                     /locus_tag="ACG8ZM_001635"
     CDS             complement(11073..12098)
                     /gene="sppA"
                     /locus_tag="ACG8ZM_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0492594.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12111..12401)
                     /locus_tag="ACG8ZM_001636"
     CDS             complement(12111..12401)
                     /locus_tag="ACG8ZM_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492595.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12810..14615
                     /gene="glmS"
                     /locus_tag="ACG8ZM_001637"
     CDS             12810..14615
                     /gene="glmS"
                     /locus_tag="ACG8ZM_001637"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0492596.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14687..16042)
                     /gene="glmM"
                     /locus_tag="ACG8ZM_001638"
     CDS             complement(14687..16042)
                     /gene="glmM"
                     /locus_tag="ACG8ZM_001638"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0492597.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16066..17235)
                     /locus_tag="ACG8ZM_001639"
     CDS             complement(16066..17235)
                     /locus_tag="ACG8ZM_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0492598.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17232..18119)
                     /gene="cdaA"
                     /locus_tag="ACG8ZM_001640"
     CDS             complement(17232..18119)
                     /gene="cdaA"
                     /locus_tag="ACG8ZM_001640"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0492599.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18308..22000)
                     /gene="nifJ"
                     /locus_tag="ACG8ZM_001641"
     CDS             complement(18308..22000)
                     /gene="nifJ"
                     /locus_tag="ACG8ZM_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0492600.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22199..22720
                     /locus_tag="ACG8ZM_001642"
     CDS             22199..22720
                     /locus_tag="ACG8ZM_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0492601.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRCLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6330 a         4939 c         4046 g         7421 t
ORIGIN      
        1 gaaaacccag cctcttcttt tttatttcta tgcttctgct actgcaggag cttttctcaa
       61 taaaccataa atcacaccag atacgactgc tccgatagca ataaacacca aatacatgac
      121 cgggtgactc agtaagaata caacgaaaat cccgccgtga ggagccagta atttgattcc
      181 gaaagctccg actaatccgc cagttaaagc agaaccaata ataaaacttg gaatggcacg
      241 aatcgggtca gctgctgcaa atggaatcga tccttccgtg acaaacgata gtcccatcac
      301 gatgttcgtc aaaccagcat cttgatctgt tttgctgaat ttattcttaa acaaacgtgt
      361 agcaacgaaa attgccaatg gaggtaccat tcctccagcc ataacagaag ccatcacgac
      421 acttccgcca gttgcaacag tcgtagctaa agttgctgta ccaaacacat aagcggcttt
      481 gttgattgga ccgcctaaat ctgccgccat cattccagct aataatgcac caagtaaagc
      541 agcattcgaa ccactcatac ttgtcaagaa accgttcaat gcatcattga ttgctttcat
      601 agggacattg accagcaaca tcaagaaacc agtaatcagt aagccaaaca ctggataaaa
      661 gaggatcgtt ttaattccat ccaatgattt tggtaagttc ttgaataatt tacgtaaaaa
      721 tacgatgaca taaccagcta agaatccacc aatcagtgca cctaagaatc ctgcaccgcc
      781 tgtattagct aatgcaccag cagcaaatcc aacgatcaaa ccagggcggt caccgatact
      841 tgaagcaata aatcctgcta ataccggcag catgaaacca aatgcagctt gtccgatttg
      901 gttgaaccag ctagcagctt gattgtaatt ccctagattt gataattgat catgtggcac
      961 gccaataaat tggtcgatca tgaaagaaag cgcaatagcg attcctccac caataacgaa
     1021 aggtaacatg tgtgatacgc cgttcatcaa atctttgtaa attctttgtc cgatagtacc
     1081 atccgcattt ccttcttctg aactgctttc ttttccatca ccatggaata ctggtgcagt
     1141 accactaagg gctagattga tcaactcttc tggtttacgg ataccatcac tgacaggacg
     1201 attgatcagt tcttttccgt caaagcgatt catctctact tttttgtctg ctgcaacgat
     1261 tacaccttct gcacgggcga tgtcttcagc agtcaatcgg tttttgattc cttctgaacc
     1321 gttcgtttcg actttgatct ccacacccat ttctttcgct tttttcttca aagcatcttc
     1381 tgccatgtaa gtgtgagcga tccctgttgg acaagctgta acggcaacaa cgaattttct
     1441 ttctggatga gcagaagctt ctgcagcttg tgtttcttgt tctgcttttt cttctgcttc
     1501 tttttgctct tcagctgttt ggaacaagtc ttggacttct tctggtgtat ttgcttcttt
     1561 taattttcct acgaaatctg catcaatcag caatcgagat agtgctgcta atgcttgcaa
     1621 atgtgtgtcg tttgcgcctt ctggagctgc gatcatgaaa aacagataag ttggctgtcc
     1681 gtctaaggct tcataatcta ctcctttttg gctttttgcg aataaaactg ttgcttcttt
     1741 aactgcactg tttttcgcgt gaggcatggc gattccatca ccaagacctg tagaagtttg
     1801 tgcttcgcgg gctagaattc cttctttata tgtttcgata tcggaaatcc ggccggcatc
     1861 aaacattttt tggaccattt catcgattgc gcctttttta tcagtcgctt gcaaatccat
     1921 gatcatcgct tctttgacca ttaaatcctt gatattcatg attatcctcc ttgaaactaa
     1981 tcaatttttg tgatttcaac ttctggcagc aactcatcaa tcaattcgcg agctgccaag
     2041 tcatcagaaa aagctgttgc gcttccgcaa gcaactcccc atttaaaggc ttcgattgga
     2101 tctttcgtac ggctgtaagc cccaataaag ccagcaatca ttgaatcccc tgcacctact
     2161 gaatttttca atgggcgaac taatacattc gttttataag ttccttgttc cgtgaagagc
     2221 aaagcaccgt ctccagccat tgagatcagc acattctgtg ccccttcagc tagtaatttt
     2281 ttcccatacg gaatgatttc ttctacagat tggaaggttg tgttgtaaag ctcagctaat
     2341 tcgtgattgt ttggtttgat caacaacggt ttcttaacaa gtgcattttt taggtcatca
     2401 cctgttgtat caatcacgaa atctgcacct ttttctcgaa tgatatcaat cagttcttga
     2461 taaaatcctt ttcgcaggct agctggcgcg cttcctgaca atacgacgat atctccttgt
     2521 cctactgttc ctaatgcttg cttcaactca tcgatttctt catctgaaat agatgggcct
     2581 aagccattga tctcagtttc agtgtctgat tttagtttga tattgattct tgtatcttct
     2641 tttacggttg tgaacaaaga ctggatttgt tctttttcaa gccacttcga aataaattcg
     2701 ccagtgaacc ctcctaaaaa ccctagtgct gtagattgag aatcgatccg tttcaaaata
     2761 cgggaaacat tgatcccttt tcctccaggt aacttgaagt cttccttcat tcggttcaaa
     2821 tcgcctagtt ttaacccatc tacgcgtacg atataatcaa ttgatggatt aagtgttact
     2881 gtgtaaatca ttgtattgcc tccttaattg ttgtcttttc acggaattca ttaacgaatt
     2941 ctaatggaca acgctcggtt atgatcgtgg ctgcttccaa aggtgcaaca gaggtgaagg
     3001 acaactggtt gaatttggta tgatcaatca atacaaatgt ctcttcggaa tttttcatgg
     3061 ctaaacgttt aacagctgcc tcttctggat ccggagtcgt gaatccttgt tcaagatggg
     3121 caccgttcat tcccataaat gctttattaa aatgaaaatt ttttagttgt tctatcgcat
     3181 tagatcctaa aacagcatta gttgccagct ttatcgttcc acctaaaata atggtagcaa
     3241 gctccaaatc aactaaattt gccgcatgtt gaacggaatt tgtcactact gtgatctgtt
     3301 ttccttgcag aaaaggaatc atttctaacg tagtagagcc agcgtctaaa taaatgacat
     3361 cttcgtcatt aatggtttca gctgccgatt tggcgatttg ctgtttttcg tgaacgtttt
     3421 tgagtgattt ttcactcata ttctgctcaa aattcaataa ttgctctttc ttagctccgc
     3481 catgaactct ttctagaaac ccactattct ccagctcttg taaatcacgt cttatcgttg
     3541 attcagaagc attcaaaagg tttacaagat cttgtgattt cacgattttc tgctgttcaa
     3601 gcaagtctag gattttttgt ctgcgttcct ctgtaagcat tttcacgatc acctcaatac
     3661 aaataatagc atattttttt tctactagca atataaatca gtcaaaaacg ttcataaaaa
     3721 ttataaatca gtcaaaaaaa aaataaaaaa ccagtcagaa agactggttt tttatttttt
     3781 tgtctatcat caagccgttt tatcgttttt tattttcgct tcttgttttt ttcatataaa
     3841 cagaagcagg ttttctttcc ttatgaagaa gataataata aagtcccatc ccgccaaaca
     3901 tgaccaaaaa accgatgact gatcgaatag gaaaattagg aacaaaaaag agacagacgg
     3961 ataataatga tgccggaacc attattcgat aatagagttg tttatccatt ttcttcacct
     4021 catatttttt gtaaagtaat caattgttct aattctcggt aatctaaaaa ttgcacacgc
     4081 agatgctgct cacctttctt gacatcgaat tggatacttc gccgcttttt ctttattcgt
     4141 ttgaaggtaa catcagagag tctatgtttg atttccttta acggtaattc tgacagggat
     4201 tctgaaggaa catagattct taaggataaa agatactctt gatatttttc atctgttacc
     4261 tctacttcta gatattccct aatataataa caataagctt cgattccatt atccaaagca
     4321 acagtcggat cgataacttt atagcacatc atgcgtgatc gattctcgtc aacccaagga
     4381 aaatacgcct gagcatttag tgttccgcga acacgccttt ctttataatc tccttgttgg
     4441 atatcataac tttttgttac aaaaaatgaa gcattctttt taggaacagg ttgcatttgt
     4501 gcattctgat actggacaga ttggatcgta tggtcagtgt aaatgtattc aacgaatggc
     4561 ggtttcaata aaccgacacc attgtcccaa gcaagatgat caaacccaag ctggtcccta
     4621 aaataggatt gaaacatttg attgtttggc ttttgccagt taaaggggtc tcgataaagc
     4681 tggatacata tgacttcgtt ttctaattcc ttcacttttt ctacttgttg gatgtcgaga
     4741 aaaatcggtt tagggatgat ttgatgataa tagcgactat aatacatttc ccaacacgaa
     4801 gtgaaacata aaccttggta aaaaagatcg tatcctggaa attggttgca atcgactact
     4861 acttccccgt tttgttgttt cattttaggt aaatgttcga tttcatcctt tttctcaaac
     4921 aatagccgtt gagagatctg ttttgtattg tgatagataa actcgctata agctcgcata
     4981 taggcgaaca ccccatgagc aatcattttt ttctgaatga agtctcgaat caaaaattgc
     5041 ttttctttga aaacagagat agacaataca ttttttatca ataattgatt atcttccaag
     5101 cgaaaactac attcgttctc gtcatttcct acaactaacc aactttcgtc tcttaccgat
     5161 gctagcatta ttttttcttg aagctttcga agatcatacg gatccgcttg ttgtgtgaaa
     5221 gagcggataa atgttggctg gatcaacaga cgtgtcaaaa tagcccattc gcttatttcc
     5281 tgtttttttc ttcttcattt aagtctgtga gctggatagt catcaataat ttatcttcgc
     5341 caatcatttt ctcacctctt tcaaatcttt ttataattta attataacca atgataaaga
     5401 aaaactaaaa ctttatgtct tagaaaccaa tttggacgat tacatgatag actaagtaaa
     5461 aagaaacaaa gaggtgttta caatggggtt gaaatttgaa cgtacagata atttagataa
     5521 acttttcgaa tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag aagagatcga
     5581 tcgatttcta aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt
     5641 taagctcaaa aaaagactta ccagccaaac ttttatgaaa gagtttgact ggtaagtctt
     5701 ttttattctt tttatgattc aattgcttgt tcgattgctg cccacgcttc ttcttttccc
     5761 tgttttgtaa cggaagaaaa gagaataaac gtgtcattgc gatcaaaatc caacgctttt
     5821 ttgatttcgc tctcatgctt gttccattta ccacgaggga tcttatctgc cttagtggca
     5881 acaacaatta cagggatatc ataatacttc aaaaattgat acatctggat atcttcttga
     5941 gaaggcttat gacgcaaatc tactaaagaa acgaccgcac gtagctggtc tctagtcgtt
     6001 aaatatgtct cgatcatctt tccccatttt gctcgctcag ttttggatac ttttgcatac
     6061 ccatatcccg gaacatcgac aaaatgtaat gcgttctcga tcaaataaaa attcaatgtc
     6121 tgggtctttc ctggcttacc ggaagtacgg gctaaattct ttcgatcaat caatgtgttg
     6181 ataaaagaag atttccctac atttgatcgt cctgccagag ctatttctgg caattgtgta
     6241 tctggatatt gttttggtgc cactgcactt atgataattt cagcttgatg gactttcatg
     6301 gtctctctcc ttaacaaggt gatgaataaa gcaagcggct gtcgcaaaag ccaaaatgac
     6361 ttttacggca gcctcttttc atcaacccgc ttttttctct gaatctttat agattactgt
     6421 tggctcacca gacaattcag ctgcttcttt tgtgataatc actttttcga tgttttcatt
     6481 tgaagggata tcaaacatca catccatcat gatttcttca atgatggaac gaagtcctcg
     6541 agcaccggta ttgcgttcga ttgctttttt cgcaatggct ctcaacgctt caggttcgaa
     6601 ttcaagttcg gtattatcta gagaaagaag tttttgatat tgtttgacta gcgcattttt
     6661 cggctcagtc aagatccgta ccagatcatc tgttgtcaat ttctctaaag ctgccataac
     6721 aggtaatcgt ccgataaact cagggatcaa tccgaatttt agtaaatctt caggaatgat
     6781 atgctgcatg acactttcgt cttcagaaag tttttggttt tgtgtaccaa atccaatcgt
     6841 cttttcgccc atacgattct tcacaatcgt ttcgatgcca tcaaatgcac cacctacgat
     6901 gaacaatatg ttcgtcgtat caatctgaat aaattcttga tgtggatgtt tacgtccgcc
     6961 ctgaggtggc acacttgcaa cagttccttc tagaattttc agcaaggctt gttggacacc
     7021 ttcgccggaa acgtcacgag taatagatac attttcgctt ttacgcgcaa ttttatcgat
     7081 ctcatcaata tagatgatcc ctttttctgc aagttccaca ttgtaatcag cagattgaag
     7141 caatttaagc aaaatgtttt ctacatcttc ccccacataa ccagcttctg tcaaacttgt
     7201 cgcatctgcg attgcaaaag gtacattcaa tgtttttgct aatgtttgtg ctaaaaatgt
     7261 tttaccagac cctgtaggtc cgatcaagca gatattgctt ttttgcagtt caacatcatc
     7321 tgtgttttct tgtgcttgct gattgacacg tttatagtga ttgtatacgg caacagctaa
     7381 cgagcgtttt gcgcgatctt gtccaatcac gtagttattc aatacttcaa gaatttctag
     7441 tggtttaggg acttcagtga attcgcgtac agcttcttca taaaattctt catcaatgat
     7501 ttctttacat aggtcgatac attcattaca gatgtagacc cctggaccag ctacgatctt
     7561 tttcacttct tcttgggttt tcccgcaaaa agaacagcgt acggtctcat tgctacttgg
     7621 attgtcatac atggtcttca ccccttaact tgcaaaaagt atgcaagaaa ctatcaaaga
     7681 ctttacagtc ttttactatc ataacatagt ttagtaaaaa gaaaaagtgt ttaacaaagg
     7741 atcaactgac ctataccttt gcatttttta ctcttaacaa aaaataagga actgtgacaa
     7801 aatcgtgtca cagtccctta tttcgaataa acaattttct agaagtagct tcttcagaaa
     7861 tgatttatta cttgatttct attctttcgc ttctgtcgct gtacctgtga tcaattcgat
     7921 tgcttttttc attgtcacat catgttccag catatcttct gtcaacacgc gtttgatttg
     7981 ttcgattggc atgttgtatg tttctgataa ttcttggatt tctgcattga tttcttcttc
     8041 tgttgcttga agattttcag cagctgcaat tgcttcgatc acaagatttg ttcttgtgcg
     8101 catttctgct tcaccttcga attgtttatg caagtcttct tctgtagaac cagttaattg
     8161 atagtacatt tcaggagaga taccttgacg ttgcatgttg ttcaagaatt cgtccattga
     8221 acgatgtact tcatcatgaa ccatcacgtg tggcaattca acgatttctg cgttttcaac
     8281 agccatacgg atcgctgctt catcttttgc atcgtccgct gctttttctt ttgtttctac
     8341 taattctttg cgatattttt ctttcaattc gtctaatgtt gatacttcgt catcaacatc
     8401 ttttgcgaat tcgtcatcta attcaggcac ttcttttgct tttacttcat ggattgtcac
     8461 tttgaagact gcttcttttc cagcaagatc ttctgcttga taatcttctg ggaaagttac
     8521 atttacatct agactttcac ctgatttatg accaactaat tgatcttcaa aacctggaat
     8581 gaatgagcct gaacctaatt caagtgagta attttcgcct tttccgccat caaacgcttc
     8641 gccatctaca aagccttcga agtcgattac tactgtatcg ccattttcag ctgcagcatc
     8701 ttctttgata actaattcag cttgtgcttc ttgttcacgt ttgatacgtg catccacgtc
     8761 agcatctgtt acttcacggt cttgtttttc aacaactaga tctttatatt gtcctaattt
     8821 tacttctggt ttcactgtta cttcagcagt gattacccag tcttgacctt tttccatgct
     8881 ttcaacatcg atttttggtt gagaaacagg atcgatgcca gcttctttga cagcagcttc
     8941 ataagcttct ggtaatacag cattcaatgc atcttcatat aaagcttctt caccgtacat
     9001 acggttgaat acttgacgag aaactttccc tttacggaat ccaggtacgt tcaagctttt
     9061 tttcacttta ttaaacgcag ttgttaatcc tttttggatt tcgtcttgcg caatcgtaaa
     9121 ttttaaaaca ccatcattcg tgcctttttt ttcaaatgtt gcagtcattt aattccctcc
     9181 gagtttagaa attcatttta tacatcaaaa acgtttcatg cataccttta aatagtacac
     9241 taaccctttc aaattgtaaa catattgaat ggatagaatc cttgtttttc ataacatttt
     9301 tatctttttc actgataaaa tctaatggaa gaagatcatt tgctgcaaaa ttttttctct
     9361 tttttttaaa aaatcttgtt tttcatcttc tgtaccttca tacatcgttt ttcctgtaca
     9421 cgcctctaca gtcagttgaa accagcgctt cgggtcttcg atgtatgtat catagacagg
     9481 ataaacaatt gcagattgca acgtgaattc ttcttttaac acggaactaa gcagcgcatc
     9541 ttcattcccg taacgatctg aaaatacaca taattgctgg taacttgcct gctgctgcgg
     9601 ctttggcaga tcttttggta tgactttgac taacttttct tcaatcgtca aatagaaaac
     9661 aggatggcat tcgttcaatt gcctaagtgt ctccagtaat tttgcccttg ctaaaatatg
     9721 gacctctggc gatacaataa gtttactcgc tgtttggata aactcatgat aaggaagctg
     9781 ttcgatctca tcaatagacg caagctgacg tgcaggttcc aacgaaggaa gttcctccga
     9841 tttattgtgg agtgccttga tcttctgcct ctccatttgt ccggataatt cttctgcaaa
     9901 ttggattttt tcaatgaccc gctgctcgaa agcttcataa aaatctgtac ttgctatcaa
     9961 ttttctagca tataaaaaat cttgtgtact gattaacaag tcaaaataaa attcagcaaa
    10021 ctcctcattt tgaagatact gtttttcatg caagagtgct tgcttcaatg cttctttttt
    10081 ctcgtccaac tcgtaaagac aaaaaacaag caggctatta gcctcaaagc tttctttcaa
    10141 ctcgtaagct cgttgaaata attcttttgc ctgctgatat tgctggtttg ctagtgcttg
    10201 ttgtgccact cgcattaaat tctcatattt ttctggaaaa tcaatcggct tcacgcagtg
    10261 tcctcctctt gctcatattc agctcatgtt caatagtagc aaaacaaaaa aagcatgaca
    10321 attaaaaata actgaagcac cctctaaaag ctagatttta gatctgactt ttagaaggtg
    10381 ctttcaactg ttactgcttt ttattgctca tcagctgttg acttcctggt tgaacgcttt
    10441 gatcagattg atcgtaacaa cacttgtatt agtgaaaaaa tcgcccagat gttgcttttt
    10501 ctgattaaag actgctggaa gataccctaa catcagcaaa ggaaatttta gaatgaaccg
    10561 gcaagcacct tctctaacta aaacggttga ccagctaagt tcttcttcat caaagctgat
    10621 gactcgaata ccaaagatca ttttcccaat cgtttggcca tgattgaatt tcgtcaataa
    10681 aataaaatac gctagataaa taagtaaggc tgtgaatccg tagagactca gataagaatc
    10741 tgaaaaatta agccaaccta gatttttggc aataccaagt gttaaacgcg taatagctcc
    10801 gatacacaaa agatcgacca agaaggcaaa cattcggata aaaaaacctg catagaaata
    10861 cttaggaaaa tcatttgctt ttggcggttc atttcttgtg tccgtatact ttttccattt
    10921 acgttgtttc tttttgattt cttcgggcgt caaaggctct ctttcaaaag agctcagtac
    10981 cttttgggaa aagttggagt caggtcctac agcaggctgc tgttgcttat ctttttcttg
    11041 ttccttattt tcatttgaaa gctctgacat gcttactcac ctccgtaata atacattgct
    11101 tttggtgatt ccgctgtccc tagattctca agaacggata agatctgact agtctcagaa
    11161 ggcttcaatc cctggaattc tgctagttta cttcctaacc atgtactagc aaacccagat
    11221 ggcgtggaag aatattctac tagttccgca tcttcaagct gttgttcctt tcgtatagct
    11281 tttaaagcat cttcaggaaa tcccaattca tcgactaaac ctacttcttt tgcttggaca
    11341 ccatcataaa tccttccatc agcaattttt ttgacttctt cttcagattt attccgtccc
    11401 tcgctgacga ttgctacaaa acgactatat gcgctgtcga tatatgcttg aagcaccgct
    11461 tcttcttctt ttgtctctgg tcgcgttcct gaacccatat ctttcaacgc tccgctttta
    11521 actgttgtat cttccacacc cagtttttct agaagtcctg aataattcaa tcctgacatg
    11581 atcacaccaa ttgagccggt cactgtttct tctgttgcaa agattttatc tgcttgagca
    11641 gaaatatagt agccaccgct agctgccatg tttttcatgc tgacatacat tgggatatca
    11701 tgttctttac gaatcgtatc caatttctta gcgatttccg cactttcgta gacaccgcca
    11761 ccaggagaat ttacttctaa aaagatacct ttgactgttg gatcttcttc aatcgccttc
    11821 agttgttcca taaaattttt atgattgtaa ccttcagaag caaataaacc tgaagaccct
    11881 cccgcagcaa tcgttccatc tactgataat tttacgattt tttgcgtact gtctccctct
    11941 tccaacacat ttggagctag ttcttcatcc ccatataacc aactgttcaa tccctcaatt
    12001 tgatcgtttt cctgaggctt agtcaaacta gctgaaacta acgaaaccac aaataatcct
    12061 gcagcaatca gaactgctgc ccaacgtctt ttattcattc tttctccttt ctaatacaag
    12121 ttttttatga cgatcacagt ttctgactgc tcagaagtgt cgaaccgctc tgctttttct
    12181 tcccgcgcat acccggaaaa cgactctttt gttaattcat cttctaccgt aaactcacga
    12241 gcttgagcaa cttccaaggc atcaggtgcg atttcttgga tgctatccca gccagtatct
    12301 aaaaatattt tcgtatcatc tgatacacca gctgatgctt cgaatttgga caattgtttt
    12361 tttaacattc ccagtgtcat gatctcaatt tttgaatcca tttttactcc tcctctatcc
    12421 gattactttc tattgtaccg aactcctgaa aaagatgata gataaaaacc cattattttt
    12481 tgacataaaa aggacatttg acaaagaatt tgatatatac caatttattt tttattcttt
    12541 ttttgttgac aagaacattc gcaaaaggtt atgattttaa atgtttccag ataaaaaaca
    12601 aaaaggagga tagttatacc ttttaggata gcgccaggtc tgtgtaaaca gatgacgagg
    12661 agagggttta tcgaaagatt cggcggatgg ccctagggac tgcactctac agaaaatcca
    12721 caaaaaatgt ctgcaaagat aaaacaaaag gattttcctg cagctggaag cattcatacg
    12781 aattcattat agaaagtagg atgtttttta tgtgcggaat tgttggaatg attggattaa
    12841 aaaatgtaac accaggattg attgacggtc tagaaaaatt agaataccgt ggttatgatt
    12901 cagctggtat ctttgtctca gatggcacaa ctgattatct ggtcaaagca caaggcagaa
    12961 ttcaaaattt gaaaaacaaa ataaatattg atactacagg aaatataggt attggtcata
    13021 cacgatgggc aactcacggg caaccttctg aagaaaacgc ccatccacat acttcccaaa
    13081 gtggacgctt cgtgctcgtt cacaacggcg taatcgaaaa ctttgaagaa cttaaaattg
    13141 cttatttagc aaatgaccac tttattggtg agaccgatac tgagattatc gctcatctaa
    13201 ttgaaacttt cgcgaaggat actacaacgc aagaggcgtt tttaaaagct ttgcgggtca
    13261 tcaaaggttc ttacgctttc gctttgattg atcggacagc acctgatgtt atttacgtcg
    13321 ctaaaaataa aagtccacta ttaatcggac tcggcgatgg attcaatgtg attgccagcg
    13381 atgcaatggc tatgcttgct catacgaagg aattcgttga gattgaagat gaagagatgg
    13441 ttaccgtcac atcagaaaaa gttattattc aaaattttgc aggtgacatc gtagaacgtt
    13501 cttcattcga agcacaagta gatgcttcag atatcgagaa aggtacctat cctttttata
    13561 tgctgaaaga aatcgacgaa cagcctatta tcatgcgtcg tatcgctcaa aaatacgtaa
    13621 cagaagacaa tcgtgtctct cttgatcaaa agctggtcga tacattgtct gatagcgatc
    13681 gaatctatat cgttgcttgc ggaactagtt accatgcggg gttagcaggt aaacagactt
    13741 tggaaaaatt gacacagatt cctgttgagg ttcatttagc cagtgaattt ggctataaca
    13801 cacctcttct ttcaaaaaaa ccattcttta ttttcttgag tcaaagtggc gaaacagcag
    13861 acagccgcca agtgctggta aaaatcaacc gtctcggcta cccttcactt acgattacaa
    13921 atgttgctgg ttctactctt tcgcgggaag catcctttac actgctattg catgctggac
    13981 ccgaaatcgc agtagcatca acgaaagctt atactgcaca aatagctgta ctaactttat
    14041 tagccaaagc tatcggtgat aaaaaagaat ttgaaacttc tcttgtattc gatatcactc
    14101 atgaattgag tttagttgct aatgcgatgg aatcggtcat cgcccaaaaa gattatttag
    14161 aagagttggc agaagtatat ctcagcgata caagaaatgc cttttacatt ggacgtggtg
    14221 ctgactacaa cgtttctctt gaagctgctt tgaaattgaa agaaatcagt tatatccagg
    14281 cagaaggttt tgccgctggc gaattgaaac atggaacgat tgctttgatc gaagaaggaa
    14341 caccagtgat cggaatcatc agtgaagaag tgactggtgc gcatactcga ggaaacctga
    14401 aagaagtcga aagccgtggc gctaaaacac tagttattgt ctcagaggga ttagaaaagg
    14461 aaagtgacca gctggtgcta ccaactgtcc acccttatct aactactcta accacagtcg
    14521 taccgactca attacttgca tattacgcaa cgctattacg gggattcgac gttgacaaac
    14581 cacgtaattt agcgaaatca gtgacagtag aataataaaa gaacaaggac cagtgtcaga
    14641 catctgacac tggtccttgt tcttttattt tttatgaaaa tatctcttaa tccagtccga
    14701 tttcttcttt gactactgcc gcgattttat cgacatagta attaactttc tcatcagtag
    14761 gcgcttctgc catcactcga agtaatggtt ctgtaccgga aggacgaact aggattcttc
    14821 cttctgatcc catttcagct tctgcctctt caatcacttt tttgattgcc ggaacatcca
    14881 tcgcaccgtt tttatcactt acgcggatat tcactaattt ttgcggatag attgttacct
    14941 ctgctgcaag ctctgataat ttcttacctg tctgtttcat gatattcagt aactgaattc
    15001 ctgaaagcat cccatctcca gttgtgttaa aatctaagaa aatcatgtgc ccagactgct
    15061 cgccgccaaa gttataatcg tttttgcgca tttcttctac aacgtaacga tcaccaactt
    15121 gtgtaacgac atctttcaaa ccgattcctt ccaccgcttt gtggaatcct aagttactca
    15181 tgacagtcgt aacgatcgta tcttttttca atcgattttt ttctgccaag tatttagcac
    15241 agataaacat gattttatca ccgtcgacaa tgtttccaag ttcatccacc gcaataatac
    15301 gatcgccatc gccatcgaaa gctagtccag catctgctcc tttttcaacg accatctcag
    15361 ctaaacgttc tggatgggta gagcctacgc cgtcattgat atttaacccg tctggatttg
    15421 tgcccattgt ataaaaatct gtttcaagat cggcaaacaa acgattaaca cttgttgaag
    15481 ttgctccgtt tgctgcatcg acacaaatgg tcaaaccgga aaggtcaccg ttgatcgttt
    15541 gtacaaggaa ttgagaatat ttcaataagc cttctggaaa ttcttctaca gttcccaatc
    15601 cttcagctga tggacgaggc aattcatcca cttcagcatc taataatgct tcaatttcta
    15661 gctcttggtc atctactagt ttaaaaccat ctgcaccgaa aaatttgatt ccattatctt
    15721 gtgctggatt atgagaagca gaaatcatga cccctgcgct tgctttttgc aaacgtgtca
    15781 gataagcaac acctggtgta gagatcacac ctagctggaa cacttcgata cccaccgata
    15841 aaagtcctga aatcagcgct tcttctaaca tttgacctga gattcttgta tcccgcccta
    15901 ctaacacgcg tgggcgtttt ccttcttctt catgctggct taggacataa cctccgcacc
    15961 gtcccagctt aaatgcaagt tctggcgtaa gttccttgtt ggcttcaccg cgtacgccgt
    16021 ctgttccaaa atatttaccc atagttgtaa aattactcct ttaacttaat tttctgagga
    16081 atttgttctg ctcatttgtg ttgattcgct tgtttctgat gtttcttctg tacttgtggt
    16141 cgagctgctt gtcccagatt ccgaactgga agaaatggtt gttgtcgatg gttccgttcc
    16201 tgaatcagca gatgaaatac ttctactcgt acttgtattt tcttgtgtcc gactgctcgt
    16261 cgaagcagag ctactgcttg acgtttgcaa cacgggtgta atctccacac taactgattc
    16321 gggcttgata cttaacccct cttctactgg tatttcaatt ttacgagttg ttcgatgatt
    16381 gatacctgag acattgatcg gtacagcaat actgtcaccg atcgatgcaa gcaaatcgct
    16441 agagccgctt acttctgctt ccagttttga aaaactgaaa tcatagcttt ctaccccggc
    16501 agctggtgtt ccttgctgta tcccataaag tcgtaccgac ttgcttggct tgatcgaatc
    16561 agctctcact gttacttgca ctggatcaga gacgatactt aatacttccc ctgctgcatt
    16621 cagcgcctgt attgggacct cactattaat cccatcatct gtgaccatcg aagcgtccac
    16681 atcagctact actcgtttga tttctgccaa tgttttgtca cctgtagtga tagagacttt
    16741 tttaggacta acggaagtat catcaagttc atatccagat ggtgtgaacg ttgaaggaat
    16801 caacgtttcc acatcgaaat cttttattac ctttttctca accgttactg taatagtcga
    16861 tggttcgatc tttgctgtga cagcactgct caaattttga atttttagcg gaacttcatg
    16921 tgttccttcg cccagcttag tgatatctgc tgtgacgcgg aacatccgtg tatcttcgtt
    16981 tgcctctgca ttcaactgaa ctcggtttgc actggaaagt ttgacagtca ctgcattttc
    17041 atatccatgg atatagtagc gctttgaatc ataagaaatc ttgatcggca cccccgtaac
    17101 tgtttgatta tacgcttcat ttccagaaag cgtatttgga acggactggc catttgcatt
    17161 gaagtacagc gcaatactga acagcaatgc aataaaactg taaagaatgc ttgtccgttt
    17221 ctcttttgag atcatttgcg atcccctccc ttagtcatac cgtctaggaa agattgaatc
    17281 acgtttttct tgttttttgt tttctcttca gcaattagtt ctctacgcaa gattgccaaa
    17341 tattcttgct gggataagcc agccatcagt tcattatcta acgtgatgct gacgtccccg
    17401 gtttcttcag agacaacgat ggtaatcgcg tcactgactt cgcttatacc gacagctgcc
    17461 cggtgtcgag tcccgaattc tttagggatc aacatacttt ctgatagagg aagataggcg
    17521 cttgctactg cgatctttcc ttcttttaca ataactgcac cgtcatgcag cggtgtatta
    17581 ggaataaaga tattgatcag cagctcccca gtgatatctg catctaaagc aatccctgtc
    17641 tcaatatact catctaatcc agtatgacgt tcgatagtga tcaacgcacc gatttttcgc
    17701 ttggacatat attggatggc tttgtcaaaa gacaagatca tcttctcatc ttcctgctgt
    17761 tcgctttttg attgtttgaa aagcgaactg cgtccaagat gttccagtcc tctccggatt
    17821 tccggctgaa aaatcacgat agcagctatc accccgtacg taatgacttg gttcatcagc
    17881 caagacaagg tgtgcagacc aatgatttcc gctacgattc ggataacaat aaaaatagct
    17941 acacctttca gaagctgaat cgcttttgtg cctcgcagca gttggatcag tttgtataca
    18001 agataccaga caaccagaat atcaagtatg ttcaccaaaa aattcagaga gaacaagtca
    18061 ccggaaaaca tttgctgcca atagtccaaa ttaaaaagtt ccccgatttg aaatgacata
    18121 aaaagcctct cttctctttt gagatttcct ctctagtata tcatatgttt taattttaga
    18181 ggctgatatg ctcgtatttt tcttgagaaa aacgcataat tttcaagaaa attgcaagta
    18241 tgcgttttat ttttctctgc aataaatatt tttaaacttc agagaattat tcattatatt
    18301 ttttctgtta tttttccttt ttttcttttg tagcttgttc cattttttta cgtcgttctg
    18361 ctctggcagc tcttctagca gctcttcttt cttctgcttc aaggtccgtc gttttttctt
    18421 ttctagtctt aacgggtaca tcgatagatt cctctggttc aagtcgtttc ttgatttttt
    18481 ctaattctcc cgtcagtgca gcatagttgt aaaaacgtgt tttagcgtcg tggacagtct
    18541 tttcaaataa agcttttgca agatttggat ttgcgttatc tagcgaggaa aagcgaactt
    18601 gtttcctcat gaaatcaggc atagcagaga aatcaggttt tttgtagtcc aaagtcattg
    18661 ggttcttgtt tttttcgacc attgtagggt tatatcgata taatgaccaa taaccagaag
    18721 aaacagcttc ttttgcttct ttaagcgatt ctttcattcc gccaagcaag ccatgtgtga
    18781 tacaaggcgt ataggcaata atcagagaag gaccaggata cttttctgct tcttcaaatg
    18841 ccttgatcgt ttgcatttga ttggcacctg aagcaatttg cgccacataa acatttccat
    18901 atgtcatcgc catcattcct agatctttct ttgaagcata ctttccacta gccgaaaatt
    18961 tagcgattgc cgaagctggt gttgcttttg aggtttgacc gccagtattg gaatagactt
    19021 cgttatccaa aacaaggata ttcacgtctg ctccgctggc aagtacatga tcgatcccgc
    19081 caaaaccaat gtcgtaagcc catccgtctc caccaatcat ccattggctg ttttttacga
    19141 atagatcctt tctcttgtat atatcctcca gttcagggaa aattgatcgt tcgctttcga
    19201 tcgcagcttg cagttttgct gctctttgct gcgttccttc actctcaaac atgtgtacta
    19261 accagtcttc catcaattct cgtagttctt ctgatacttt tggcaatact tctttgattg
    19321 aacttgccaa agcctcgcga cgagtctgtg ccgctagaag cataccataa ccaaattcag
    19381 cattatcttc caataaagaa tttgaccatg caggcccttg accacattca ttggtcgtat
    19441 aaggagaagc aggagaagca ccgccccaaa tagaagaaca gcctgtcgca ttcgcgatca
    19501 tcattcgatc accgaacatt tgtgtcaata attttacata aggtgtttcg ccacagcctg
    19561 agcaagcgcc cgagaattca agaagcggtt gattgaattg tgtagacaag acagtattct
    19621 ttcctttgac tggatttgct ttttgtctta atgtcattgc aaaagcccaa ttcacagctt
    19681 gttctttctg ttcttcataa ggcttcatca ctaaagcttt ttcttttgcg ggacaagcct
    19741 ctacacacag accacaacct gtacagtctt ccactgaaac ttggatacgg tattttacgc
    19801 cgtctgcgcc tcgcatttct ctcacgataa atccttctgg cgcttcttcc atttcctctt
    19861 catctgctaa aaatggacgg atggcagcat gaggacaaac aaaggcacat tcattacaca
    19921 tcgtacagcg atcactaatc cactcaggta cttctaaagc gacgccccgc ttttccatag
    19981 aagccgttcc taaaggcatt tcgccagttg tcattccatt tgctacaagt gtaccaacag
    20041 agagttgatt tccttcctgt gcattcactg gttcaagtat ttcttgtata taaccagtgt
    20101 tccttttatt gattttttca ggcatctcca acgttctcca atggtctggg atcgcgactt
    20161 gatgaagcag ttcggtcgtc cgatcgattg cttgtatgtt tttttctacg atagccattg
    20221 atttatgtcc atagcttttc agggcttctt cttttagcag cggaagcacc tcatcaaaag
    20281 gaatgatgtc agctaattta aaaaaagccg tttccattgc cgtattgatc cggcgtccca
    20341 aaccgacttc ttgggccaat ctcatggcat tgatcgtata gaatctaatg ttattttcag
    20401 ccaagtaacg ttttaatttt aagggcagat gcgtttctaa ttgttcgtcg ctccaaagag
    20461 tattcaataa aaaaatgcca cctggcttca atcctttgac caagtcatag ctatgcaggt
    20521 aagctggtgt gtgacaggca acaaggtccg catgctcgac aagatacgct gaacgtatcg
    20581 gcgtatctcc aaaacgaaga tgagagacag tcagaccgcc agattttttc gaatcataat
    20641 aaaaatagcc ttgcgcatat ttatcggtgt gatctccaat aatcttgata gctgatttat
    20701 ttgctccaac tgttccgtca gatccaaatc cccaaaactt ggcctggaat gtttgcggtt
    20761 ctgtcaaatc cagactttcc tttggttcca aagattgata agtcacatca tccacgatcc
    20821 ctatggtaaa ccgttttttg cgtatgcttg ggtctttttt caattcatca aaaactgctg
    20881 agatttggtc gggagtcaca tctttagacc ccaatccgta gcgaccacca ataacagccg
    20941 ggcgaagttc agaatcatat aacgcacttt gaacgtctaa taagagaggt tctccacctg
    21001 cccctggttc tttacttcga tcaagaacag caattgtttt tactgtatga ggcaactttt
    21061 gcacgaatgt ttccactgga aaaggacgat aaagatggat attcaaaaaa cctgttttcc
    21121 gtccgtttgc attcaagtaa tcaatcgttt gttcaatcgt ctgtgcaact gatcccatag
    21181 aaacaatgac ttcttccgca tcgtctgctc cgtaataagt caccaagtca taagaagttc
    21241 ctcgtaatcg gttgatctct ttcatatatt tttgaacgat ttcaggtaat ttatcataat
    21301 aggggttgat cgtttcccgt tgttgaaaat ggatgtctgg attctggttc gtaccactaa
    21361 cagtcggatg attgggattc atccctcgtt tacgaaatga agctaatttc tcctgattga
    21421 tcagaggagc taattcttca tattccaaaa cttcgatttt ttgaatctcg tgactcgtac
    21481 ggaatccatc aaagaaattc aaaaaaggga cttctccttc aatagaagca agatgggcaa
    21541 caggtgctaa atccatcact tcttgtacac tgctttcagc taacatagca aatcctgttt
    21601 gtcttgcagc catcacgtct ccgtgatctc cgaaaatatt caacgcattt gtcgtcactg
    21661 cccgactagc aacgtggaaa acacttggca gtagttcccc agcaatcttg tacatattcg
    21721 gtatcatcaa aagcagccct tgtgaagcgg tatatgtagt tgtcagtgca ccagttttca
    21781 atgatccatg gaccacacca gctgctcctg cttcggattg catttctact acttttactg
    21841 gttgaccgaa tacgttttct tttcctgctg ctgaccattg atctactaat tcagccatgg
    21901 tcgaactggg tgttattgga tatattgcag caatctcggt aaatgcataa gaaatatatg
    21961 cggctgccgt attgccatcc attgttttca tttttcgcat gattcatcat ccttgttcat
    22021 attctctctt attatctgtt ccattataac gtaaaacatg ccattttttt cttttattta
    22081 taaaaatatt ttacatgtat ctatttgaat tggtcatcta atttttagac aaacagtcta
    22141 aatcgttgcc tttttttgtc agacatgtat aaactataag cgaaagaggt gttttaacat
    22201 ggatatatca gtgattgatg caacgaaaac aaatacacaa aaaggaatcc tttatggttc
    22261 atcgaatgct cctaaaaaaa tggtcgaatt catcaactta gcttgtccgt attgccgaca
    22321 atggtttgaa gaatcttatg agctattaga agaagcagtc caaagcgggc aattgcagcg
    22381 agtgatcaaa ttgttcgata aagaaaaaga aagcttacag cgagggaacg tgatgcaccg
    22441 gtgtctcaca atcagcgatg gccaaaaagc gatcaaagag atcaaacaaa tattcgatac
    22501 tcaagacgag tggaaacatc tttctctcca agaagtagcg gattttgcag tagataaact
    22561 aaagctgaca gaacaaaaag acgaacagct gagccaagct gtcatcaacg aagctgagca
    22621 agcacatatt cgatttgttc caactgtcat cttgggtaaa gaaatctttg atgaatctat
    22681 ttcaatagag gaattaaaag aattgatcca aacaaaataa aaaaataagg agcgtg
//
LOCUS       DAZKOR010000047        21428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000047 DAZKOR010000000
VERSION     DAZKOR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.047"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..810
                     /locus_tag="ACG8ZM_001643"
     CDS             109..810
                     /locus_tag="ACG8ZM_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0492602.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1419..2435)
                     /gene="tsaD"
                     /locus_tag="ACG8ZM_001644"
     CDS             complement(1419..2435)
                     /gene="tsaD"
                     /locus_tag="ACG8ZM_001644"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0492603.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2432..2896)
                     /gene="rimI"
                     /locus_tag="ACG8ZM_001645"
     CDS             complement(2432..2896)
                     /gene="rimI"
                     /locus_tag="ACG8ZM_001645"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0492604.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2903..3445)
                     /gene="rimI"
                     /locus_tag="ACG8ZM_001646"
     CDS             complement(2903..3445)
                     /gene="rimI"
                     /locus_tag="ACG8ZM_001646"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0492605.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3429..4151)
                     /gene="tsaB"
                     /locus_tag="ACG8ZM_001647"
     CDS             complement(3429..4151)
                     /gene="tsaB"
                     /locus_tag="ACG8ZM_001647"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0492606.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4342..5322)
                     /locus_tag="ACG8ZM_001648"
     CDS             complement(4342..5322)
                     /locus_tag="ACG8ZM_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0492607.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5346..6830)
                     /gene="galT"
                     /locus_tag="ACG8ZM_001649"
     CDS             complement(5346..6830)
                     /gene="galT"
                     /locus_tag="ACG8ZM_001649"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0492608.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6842..7831)
                     /gene="galE"
                     /locus_tag="ACG8ZM_001650"
     CDS             complement(6842..7831)
                     /gene="galE"
                     /locus_tag="ACG8ZM_001650"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0492609.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8079..8246
                     /locus_tag="ACG8ZM_001651"
     CDS             8079..8246
                     /locus_tag="ACG8ZM_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0492610.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8308..10119)
                     /locus_tag="ACG8ZM_001652"
     CDS             complement(8308..10119)
                     /locus_tag="ACG8ZM_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0492611.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10116..10481)
                     /locus_tag="ACG8ZM_001653"
     CDS             complement(10116..10481)
                     /locus_tag="ACG8ZM_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0492612.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10644..11039)
                     /locus_tag="ACG8ZM_001654"
     CDS             complement(10644..11039)
                     /locus_tag="ACG8ZM_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0492613.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11057..12019)
                     /locus_tag="ACG8ZM_001655"
     CDS             complement(11057..12019)
                     /locus_tag="ACG8ZM_001655"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0492614.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12019..12231)
                     /locus_tag="ACG8ZM_001656"
     CDS             complement(12019..12231)
                     /locus_tag="ACG8ZM_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0492615.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12252..12950)
                     /locus_tag="ACG8ZM_001657"
     CDS             complement(12252..12950)
                     /locus_tag="ACG8ZM_001657"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0492616.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12970..13512)
                     /locus_tag="ACG8ZM_001658"
     CDS             complement(12970..13512)
                     /locus_tag="ACG8ZM_001658"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0492617.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13644..14648)
                     /locus_tag="ACG8ZM_001659"
     CDS             complement(13644..14648)
                     /locus_tag="ACG8ZM_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0492618.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14645..15634)
                     /locus_tag="ACG8ZM_001660"
     CDS             complement(14645..15634)
                     /locus_tag="ACG8ZM_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0492619.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15631..16437)
                     /locus_tag="ACG8ZM_001661"
     CDS             complement(15631..16437)
                     /locus_tag="ACG8ZM_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492620.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16603..17559
                     /locus_tag="ACG8ZM_001662"
     CDS             16603..17559
                     /locus_tag="ACG8ZM_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0492621.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17636..18154)
                     /locus_tag="ACG8ZM_001663"
     CDS             complement(17636..18154)
                     /locus_tag="ACG8ZM_001663"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0492622.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18242..18391)
                     /gene="rpmG"
                     /locus_tag="ACG8ZM_001664"
     CDS             complement(18242..18391)
                     /gene="rpmG"
                     /locus_tag="ACG8ZM_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0492623.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18619..19065
                     /locus_tag="ACG8ZM_001665"
     CDS             18619..19065
                     /locus_tag="ACG8ZM_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0492624.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19260..21155)
                     /gene="asnB"
                     /locus_tag="ACG8ZM_001666"
     CDS             complement(19260..21155)
                     /gene="asnB"
                     /locus_tag="ACG8ZM_001666"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0492625.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6444 a         4548 c         3699 g         6737 t
ORIGIN      
        1 atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta caaattcttt
       61 aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat gcgtgttaat
      121 aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca tttctcggat
      181 gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa aggacagatc
      241 cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg actcgttcgt
      301 ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa tacaaataac
      361 ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc tcaagcgatg
      421 actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc acgaaaaaat
      481 cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat acatgaattg
      541 cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa tttattcatt
      601 ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc ttatccgatc
      661 accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg acaagtaatc
      721 aaacaactac gtgattccgg gctgttagat tatgacaaaa agcgttttac tttctgtaag
      781 acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt gaccctgata
      841 aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag
      901 atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt
      961 tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg atctagatta
     1021 ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga acggataatg
     1081 ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg taaaaaatag
     1141 ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac ctgtcaactc
     1201 ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa ttgttccttg
     1261 tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc aaaagcttgc
     1321 atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc ctgggctgtt
     1381 cttattactt gatgcagggc agctttttca caacctcttt attcagataa aaccaagctt
     1441 ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac atgtgctgct
     1501 gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac tagttctacc
     1561 tcagggagtt tagctgccaa agcatcctgc aatccttcac gcaacccttg attggccgct
     1621 acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg aagtgtttta
     1681 gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt tttgtctaga
     1741 tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt taatccgcta
     1801 aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta attatctttt
     1861 ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc taatactctt
     1921 ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat gatttcatat
     1981 gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag aagtgccatc
     2041 aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc catatggttt
     2101 acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga aataccaatc
     2161 agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa atcttctgcc
     2221 gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc tacgtgatgg
     2281 cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat ttgagaagca
     2341 acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt ttcatcacaa
     2401 cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt tttaactttc
     2461 tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt ttacctaata
     2521 tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga cgtacttcta
     2581 aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac aaatgccgtg
     2641 catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg acttcatcta
     2701 ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa agcagataat
     2761 cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt gaaccatgtg
     2821 gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca tcaaaatctt
     2881 tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata tccaacgcat
     2941 cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac cccaatttcc
     3001 gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc gtctcacagc
     3061 gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt cgtttacgct
     3121 gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac gctcttgaac
     3181 cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga ggaagcggtg
     3241 acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta tagacttctc
     3301 gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt acgtaacgac
     3361 gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa aagcttctgg
     3421 catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt ctccaaccat
     3481 ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt ctctgcaact
     3541 ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc ccatgctgga
     3601 actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa tttgtgacaa
     3661 tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa caaatcagaa
     3721 gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca gcggtatgcc
     3781 ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt atccacaccg
     3841 atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt atctaatgta
     3901 taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga accgggacca
     3961 tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc aaacaattgg
     4021 tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg tatttgtcca
     4081 agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt atctattcct
     4141 aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat tgtagcatac
     4201 ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata catgattatc
     4261 tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta tcactccatt
     4321 ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact ctgtttccac
     4381 aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg tctccactgc
     4441 tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg ttacatattt
     4501 cgctacactg acatcattga agcccatcac agaaatatct tctggtacca ctaaaccttt
     4561 ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc
     4621 aggaacatgc ggattcgttt ttaaatattg cttcactgct tgatatcctg actcgacaga
     4681 aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa
     4741 tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt ctgttccagc
     4801 aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt tttgaacact
     4861 tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag aatctaccaa
     4921 aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga attttcctag
     4981 tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt tcaataaagt
     5041 aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat aataaagatc
     5101 ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct tagtatttgc
     5161 tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa ttttttgctt
     5221 tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa cagtagcagg
     5281 agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct cctccttttt
     5341 tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc atctcgcttg
     5401 aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt ttgtacgaca
     5461 tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc tgcccacgga
     5521 cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac ttctactagt
     5581 tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa cccaatgttc
     5641 tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg atgttcttct
     5701 gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct tcttcgtgca
     5761 attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat agatactgtt
     5821 tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat aagcaagttt
     5881 ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc tgcgatgact
     5941 tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat aggaaattca
     6001 tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat tggtaagtca
     6061 gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag tctagcaaat
     6121 gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga atgttcattg
     6181 taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt cattcgaata
     6241 atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc gttttccatg
     6301 caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc gatttgtttt
     6361 ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata ctccgactct
     6421 gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt tttcttactt
     6481 aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg tgcaaagaat
     6541 gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc aaaaatctct
     6601 tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac aagctgatcc
     6661 aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag agcatcttct
     6721 cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat ccatccgcca
     6781 gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat atctcattcc
     6841 cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt ttgataatcg
     6901 ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt tcacttgaag
     6961 cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga atttcttttc
     7021 ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg ctgctgccta
     7081 aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa atatgtgcag
     7141 caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt gtatcataat
     7201 cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa atgattggga
     7261 caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt gctccggcta
     7321 cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac cacttcatca
     7381 ttttttccat catcaattta ctttcaccat acggattttt aggattagct ggtgtatctt
     7441 ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag aatacgattt
     7501 tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat acgttgttgt
     7561 tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca gcaaaatgga
     7621 ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta tcccggatgt
     7681 ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg agcaaattat
     7741 ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc gaaccgatat
     7801 aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt agtaaaattg
     7861 tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga aaacaaacaa
     7921 agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta aacattttca
     7981 agggtttttt gcaaaggttt acaaacaaac gtaaaactag cctttttttc tcttttgttc
     8041 cataatagat ataacaaata ataaatgagg tggattcaat gaaaaaattt gtatctggaa
     8101 tattagtagg aagtctggca acagccgctg ccgttgctgg tttggttgct tctgtgaaaa
     8161 aaacagtgat cgatccaatt gacgaaaaag aagcaatgat cgaagaaaac cgtaaaaaag
     8221 caatgcgtaa acgcgtttct cgttaataat tatttaaaaa tacgcatgaa gactttctaa
     8281 aaaacagata agtcatcatg cgtattttta tttgtcagtc aatataagtt gtgctatttt
     8341 atctgcctgt ctcagattcc atggatctgt ataaggtcta gctactgctg cagttagcca
     8401 atccacacct tcaacaggta ttccatagca ataaaagtca gactggattt cccctgaagc
     8461 atcaatcacg cgattcgtac ctcgctcaac atctatagct tctgaatgat aacttcctgt
     8521 attatctgaa aatgaaaaag ggcgaacaag tttgttttcc ttcaaatttt ttatgacagg
     8581 attacttgaa cgagaaaagc ttgtttgtgg caatcgagct tcaatcaaat agcgactgct
     8641 gatttcttta tcttgtagag aaataacata gcggttgtgc ttttgctgaa tatcaaatgg
     8701 cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct gctaattctt tcgtccgaat
     8761 gattggcgga ccgatcgtta aaaagctatt tagatgagta aaccatcccc atagtatttc
     8821 tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc tgccggattg gatcacgcca
     8881 gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg caattgcctt tttccgcttc
     8941 ttttatctgc ccttcaataa atattcggct agcttctgaa aagggctcat tctgataggc
     9001 aagaggagtt tctaaatacg accaatccca tctataaggg attagtgctg gatgctttcc
     9061 gagacactcc tctgaagaga gggagagaaa atcatgctca aaatttttct ttggaatcgg
     9121 caagtgatat tcttcgatga ttttcttgta ataaaacaac tcaacgtctt ttttcagata
     9181 gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc cattgctcca ggttttcttt
     9241 tgttagtaac cgtggccatg ccattgctcc gccttgcttt tgatttcttc ctcgagggta
     9301 ataagggagc ccccttcttg atccactgta tacaatcggt tccttgcctg atggatgata
     9361 gaccagtttt cccttttcgt tctcgaagcg ccctcctctt gatactgtaa acaatccgac
     9421 ataatcaaaa aaacttaagc ctagacctct taaaaagaca ttttctttag gcggtatctt
     9481 cgaaagatct acatctgcag gattagaagg aagctgatac agtaaaacat ttttctttgc
     9541 gtagtctacc cacactcttt cttcttctgt ctcatcattt tcccagtgac cgcttgctaa
     9601 aacaagctga tcgatagaat agctttgctg atctgtctgt aaaagataac catcttttct
     9661 tttgcttact ttttttacta aagaataaac aaactgaata gaagctggaa attcttcttg
     9721 caactttgag aaaaaccagc gttgatagag tccgtataaa catcgagtag attgctgatc
     9781 tttttccaga cgattgattt cttctataat tagttgctta tttttggtta ccgtctgttc
     9841 gatataatct gtagcaggtc ctttactcca ttgaaacaag ttgggtccct gtgttaccaa
     9901 tcctccactg gacaatgttt catctgtaaa aagcgtcact tgttgagaaa cagaattcat
     9961 taataactcc ggtggctgat tcaaccgcca aactcgacct ccaggaccat tgggatcgaa
    10021 aagaaaaagt tcaagagaat gtctgtcctg attattatgg agaatccttt ctgctacact
    10081 taatcctcta ggtccggcac caatgatccc tattttcatt tgcatgcctc catttcgata
    10141 aattttttca ataaaacagc atggttctga tgttcatttt ttgcaccata aacaaaagta
    10201 atgactggat gttctttcat aagttgactg agtttaaaaa tagcttgctt cttttcttct
    10261 tcgaaaatca attcttgctg gtatttcttt gcaaatgaag caaatcgttc tggtacatgg
    10321 tcaaaagttt tgcgtgcttc attactaggt gcaatttctt tcaaccacag atctaactgc
    10381 gctttttctt ttgaaattcc tcgtggccag aatctatcca cgagtattct atagccatcc
    10441 tctttttcag catcaaggta aacacgtttt aatttgatca tagttgtttc cttctttcta
    10501 ccttttcgct tagcttatat atgtagataa tagcacaaaa taaaaaaaat gaacggcaca
    10561 actatccatt gcgccgttca tgtgatcttg gtcaaaatct aatgattgat tcggttttta
    10621 aagctgtcat acaatcatct gttttatact tttttacttt tgatttttcc agaccatccg
    10681 tcatatccac ccttaagtaa ataaatctct tgataacctg ctttacgtaa tttatttgct
    10741 gtacggatgc tcaaactttt cttttgatcg taaatataga caggctggtc ttttcgaata
    10801 gatcctaatg aatctttcaa aacagtgtaa gggatgtttc ttgcacctaa aatatgccca
    10861 gcatcaaaca catctttttc gcgaacatcg attacttgtg cttttctcat tccttctcga
    10921 aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca ttactcgtag atagacttcc
    10981 caaaggatga ttgcaagcac aatcaatagc aaaatgatat taattaccca taaaaccatg
    11041 taatgcctcc gtttttttaa gcaaatttca aggctaatga ggcagcacca atgacaccag
    11101 cttcattacc aagctctgct aatttgattt ttgttgattc tcttacttgc gggaaagtaa
    11161 attctttaaa atatttctct acacggctac gtaaaaattc tcctgctgcc gatactcctc
    11221 cacctaacac gatacttgac ggatttaatg tattccccaa gtttccgcaa gctaaaccta
    11281 agtagaagca tacgcgatca acaaccatca atgcaaacgg atcgtcttct tgagccattt
    11341 caaagatatc tttacttgtt acttcttctc cgttgtctag cattgctttc agttttgaat
    11401 cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac cacacctgta gcgctagata
    11461 cagtttctaa acatccgcgt ttaccgcaag tacattcgaa cccttcagga tccacagtga
    11521 tatgtccgat ttctcctcca gctccgctca cgccgtgaat caaatttccg ccagcaacga
    11581 tacctccgcc aacacctgtt cctagagtaa cgaatacaac gtctggatcg ttttctccag
    11641 cacctttcca tcgttcacct aaagcagcaa cattcgcgtc attatcgata gcgaatttga
    11701 ttcctgttcc tgcctcgatc agttttttca caaattgaac ttctgtccag tttaagttat
    11761 acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc tggggtaccc atgccgattc
    11821 cgataaagtc ttcggctttc atattatata aattcaaacg atgattgatt gattcaatga
    11881 tttcaggaac gatatgctta ccatcatcca aaatattggt atcgatgctc catttttgct
    11941 gaatctctcc ttctggtgtt aaaattgcaa attttgctgt tgttccacct aaatcaatcc
    12001 caataatctt cttgtccatt acaaatttcc tttctttcgg ctttcttcaa ttcggtgctc
    12061 tctttttaat atgcgccacg ctgtcatgta tgtatcatga tcaatcaaac ggctatcata
    12121 cagattacgc aattcgatag tcattaattc aatgtcatag atgcgtgcac ccacatagat
    12181 aaagatgcca aattttttga acagctgctg tacatcatat aagctctcca tctttaagca
    12241 cttccttcat attatacaag taaaccatat ttttttaaac ccagtaaaat acatattaca
    12301 aaaagaaata caaaaactag tgtagaaaga atcctttggt gtatcgaata gcttgtctgt
    12361 tgtttcggta gacccaaaac attccctaac actaatccgc caattagccc accgacatgt
    12421 ccccaaacat ctacagaggt atccagaaga ccaaaaacaa atgtcaacac aataaataaa
    12481 agaaaatgtc tgactagctg ctggatcgct gggttatgtt taaaatgaaa ccctaagata
    12541 aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac caccagacag aacattcgct
    12601 tcgttaaaag caaagcttgc tgtatttccc attatcccgc tgaacatata aatcaagaaa
    12661 aaacgccaat ggccatagat tgcctcgatt tgctgcccca taaaatacag tatcagtgag
    12721 ttcattacga aatgcatcag tccaaaatgg atgaagattg gtgtcactaa acgccaccat
    12781 tcattgaagt gaacaagata aggaccatac attccggcat aataagggat ctcaagtcgc
    12841 ggaaataatt ccatcatgag gaagaccgct gtctgtatcc ctaaaaaagc ataggtccat
    12901 aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt gataattcat gaaaacctcc
    12961 catttcattt tagtcaataa acaattgttg gatcttctga tcaaagctct ccggttgcca
    13021 ttgttcattc aattgttcac gaaaaacaag gctacatgtt tttttatgaa aattatttaa
    13081 ataccggtcg taataaccac cgccaaaacc gattcgataa ccggctctat tgtagataat
    13141 acctggtaca atgaggagat cgatcatctc cggcaaatag agcttctctg attgtggctc
    13201 aagaacacca aaactcgaac gtacaagaac ggactgttca tccatttcat aaaacgccat
    13261 ctgacgtttc ggcaaagttt taggtacaac tattttcttt tgttcttttt ctgctcgttg
    13321 gataaccggc tgtgtatcca gttcaaattc attcgataaa gtcgtcccta tcacttttgc
    13381 tgacttccag tactcgctgg cgaatagctg tttatagatt cgctgttctt tttccttttt
    13441 cttgccactt ttggccagat ttttcagctt ttcgattcct ttccggcgta atcttacttt
    13501 caattggtcc aaaaactcac ccttccttct ctcataaaat ggtataggaa aaaaagaaaa
    13561 atagcaatcg aactttcaaa taaatgcata aatagaataa aaagaagacc tagaaatgat
    13621 tggccttcct tttttaatga atttcaactg ttcagcgcgt gtttttgtct gataaaaagt
    13681 gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa ttcctgtagg tacttcccca
    13741 cttggtagaa tcactctagc aaccatatct gcactaagca gaaaaatgga accgaacaaa
    13801 ccgctcagaa caaaggtcca gttattttct ttacgtacga ctagcttcgc agcatgagga
    13861 gctagtagtc ctacaaaaga taaactacct gtcaccgcta cactaccgct tgccaaagca
    13921 acagctaaca acaaaaggat tttttgactt ttagccacgt taaggcctaa tccttgcgcg
    13981 cgttcttgac caaatgatag caatacaatc ctctgtgcat aaagtcctcc tagcggaatt
    14041 aagataaccg cccatggaag caatgcccaa acataaggcc agctcgcacc ccagattgat
    14101 cccgctaacc atgcattcac aaatccataa ttttccttag ataattggat cgtgcatagc
    14161 aatgttaatg cagaaaggcc ggcattaacg gcaataccgg acaataagat tcgattcata
    14221 cccaaaaagc catttcgatc ataggccgca agatagacac agcaagcagc taaaacacca
    14281 cctagaaagg ctacaactag caatagaaac ggcagcgaat agtgcgaaaa gaaacctaaa
    14341 taaagcatca ctaaaaatcc tgctccggca ttaatgccca atatactaga atctgccaat
    14401 tcatttcttg ttactccttg caagatataa ccagaaacgc caagacaagc tcctgctaat
    14461 aaactgatca gtaatcgagg aactcgaaat tctctaacaa tcagcgccaa agaagatgat
    14521 tctttcccta tgaacgtggc aaaaagctga tccaggtaaa taaaaggcgt accaatcatt
    14581 aaactaccta tagacaggat gacaagtaca aaaagcaaga aatagaacgt ccaatatatt
    14641 tttttcaccg ttgttccctc cttacttgta tcagtaaaaa cggaacgcca atcaatgcaa
    14701 tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat cctcgctgta aaatccgcca
    14761 acataaccaa tccaccgcca actaaaaaag agacaggtag aacataacga tagtccgttc
    14821 caaccattcc gcgaacaact ggcggaacca acagcccaat aaatgaaaca ggtccgacta
    14881 acgaaacggc aatggctgct aacactgcaa ctagacagaa aacaatcaac cgcgttcgtt
    14941 taggatgact tccaagagct aatgcatgtg tttcactcaa atgaatcaaa gatattgctt
    15001 ttcctaatga aaaagaaacc aaaacagcaa gtatatataa aggaaacaat actgctaatt
    15061 gtttccatgt gatattggca gctccgccta caaaccaaaa agcaagatcc tgctgcagat
    15121 taaagaattg ggtaagtgct tggctgatag cagtaaaaaa agaactaata gctacgccag
    15181 ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa ctgcaaaaaa ccaaatacga
    15241 gcaaagttac agcaaaagaa cctgccaatg acattagaaa cacagaaaaa tcagaagctt
    15301 taggccaaaa agcaaaacta agggccattg ccaacgaagc acccgcgttg attcctagta
    15361 agcctgaatc tgctaaagga tttcttgtca ctccttgcat caatgcaccg ctcaatgcaa
    15421 aacaactccc tacaagaaat gctcccgcca cacgaggcaa acgaatattt cgtatgattt
    15481 ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg ccaaaaatct gctttttcca
    15541 cagtcgctgc gccatttgaa agagcaacaa aaaaaataat caaaagaaca aaacagacca
    15601 aaagcaggag tctatttttc ttcatagtcc tcataattga tcctcctgct cttttggcaa
    15661 caaatcatag gttagaaaaa ctggtttatt tgttcttgga agcgtgacaa tcgttgcatc
    15721 aatctcaaat atttttttca tacactcttt tgtgatcact tcttcaggtg taccttctgt
    15781 gatcaactta ccgtttttca ttgctaataa ataatctgaa aaacgtgaag catgattaat
    15841 gtcatgaatc gacatcaaga tcgttgtttg tttcatttga ttgatttcag tcaataactg
    15901 taggatctct aattggtgtg ccggatctaa aaatgttgtc ggttcatcca agatcaagat
    15961 atctgtttcc tgagcaagcg ccatagcgat ccagacacgc tgggtttgtc caccagacag
    16021 cgttgcgata tcccgcatac gaaatccagc tagacctgta gcatccaacg cccactcaat
    16081 tgcttcatga tcttgcgtgt ccatgccact gaacagtttt tgataagggt atcgtccgta
    16141 agatactact tcttctactg tcatacctgt agggtgttca ctagtttgtg ctaataatgc
    16201 aatttttttt gcaagttctt ttgaactgta tgtagagata tcagagttat tgacaatgat
    16261 ctttcctgat ttcacaggta gtatacgggt aaaacttttc aataaagtag attttccact
    16321 tccatttggc ccaatcaaac tagtgatttt cccttcagga atcgtaacag acatgttttc
    16381 aatgattttt ttgttttcgt agccggctgc aatgtcagct gctcttaatc tcgtcatttc
    16441 acccatcctt tcgctgaaaa tcattctcaa ttatgacgct gtttcaacat ttcgtcaagc
    16501 gctactttca tatctatagc caaatgaagc ggttgacagc gttcgaaata tcaggaataa
    16561 taaccaatga gaaagattct caattatgtt aggaggaaag aaatgaaatt aaaaacaaaa
    16621 gtattcacat tatctagcat attgctgctg gctggagtac tgagcgcttg ctccccctct
    16681 tcagataaag aaacagctac aactgccaat tcgacgactt catccaatag tactcataca
    16741 gtcacagata cacttggaca taaagtagag attccaaacg cgcccaaacg gatcatcggc
    16801 agttacttag aagactatct gattgcacta ggggaaaaac ctatcgccca gtggactgtt
    16861 ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa aggatgtccc aacgatttct
    16921 tatgaccttc cttatgagaa agtgttaagc tttgagcctg atttgctgtt gatttcttct
    16981 tccgctactg tcgaaggcgg gaaatacgaa caatatagca aaatcgcgcc aacttatgtt
    17041 gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa aagatgtcgg caaagctttg
    17101 gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc aaaaatccat caagaaaact
    17161 cgagaagaac tagcagataa aatcgagaac aagacagctg cagtgttgtg ggtgaccaac
    17221 aactcagcct ttatggtcag caaaaatcgg tcaagtggaa gaatcgtata cgacgatctg
    17281 gaatttggtg ttcctgatct agtggaagag gtctccaaaa aagccacttc tgactggtct
    17341 gctgtttcct ctgaaaagct agctgaatta gatgctgact acatcatcct tgtgaacagt
    17401 gacaaagatg ctgccatgtt caatgaacca agctggaaga atctaaaagc agtgacagac
    17461 aatcatgtac tgcaattcgg acctgaatct agttggctat ataacggacc tattgccagt
    17521 caggaaatgg tcaatgatat caaagaaaaa ctaaattgat tttaactaaa aaaagcatct
    17581 tcacgattag tcgtgagatg ctttttttct tattaacaaa ctagacgcat agattttatt
    17641 cttcttttga agcaacccta ttttttactc tttcccattt ttgaaaacga tgggcatata
    17701 gattttcttg atccgttatc ccctcatact cctctataag ggcgaagtct gaaaaatcaa
    17761 tattcccaat atacgtatcg ccttcaaacg tgctatcgat caatgttcgc cagatccgac
    17821 cagtgtaagg aagaagttct tgaaaaatcc gactaccacc cgaaacatag atatcttctg
    17881 agatcgtttg actatacgac aaaagttcat cgatcgaatg cattacttcc gcattttctt
    17941 tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg gcgataagga agtgaaagat
    18001 cccccattcc ttcataggtc tttcttccca tcactaaaat tcgatccatt gtatgttcgc
    18061 gaaagaaacg catatcattt ggcaatcgcc atggtaacaa accatttttg ccgatcagac
    18121 catttttatc ttgtgcccac atggaaataa acatattatc cgctccttat gattttagat
    18181 gttttgagat aaaaaaagtc tcaattcttt gaccagcaag gaattgagaa ctttaaaaac
    18241 attattttgt ttcacggtgc aaagtaactt tgcgttcgcg tgggcaatat ttttgttttt
    18301 ccaaacgatc aggattgtta cgtttgtttt tgcttgttag atagttacgt tctttacaag
    18361 aagtacactc taaagtgatg tttacgcgca tgtttttccc tcccattttt acaagaatcc
    18421 tctaagatga ctctcagact tgattatctt accatgattt tttctagatt gctagtgttt
    18481 ttcacgattt tgttaagtaa aactacttga cgataaatac aacggtttta cttctcaaac
    18541 aaaatctttt tctttttgta taaataccct tacacttgtt tatatagatt atgtttttta
    18601 ataaaggggg atactaatat gccatggaac atgaaggact ttcctgcatc aatgaaaaac
    18661 ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa atgcattatt agatgaaggg
    18721 taccctgata accgtgcgat cccaattgcc attgatcaag caaaaaaatg gtatgaagat
    18781 gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa atccaagcaa gacggatgaa
    18841 cacgatacga atcctcgtgc tggaaaactg cttgattcag atgtgatcgt agaatatgag
    18901 gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg caagcaacca ttttgataca
    18961 aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa acaaagaatc aactttgttc
    19021 atttataaaa aagatgggac aaaagaaaaa gaaatcgatt attgaacata tgtaaagggg
    19081 ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa caatggtgtt cctctgccaa
    19141 ctccgaacca taagtcataa tatctataaa acgggaagag ctgttttttg aaattgtttc
    19201 ttatcactca gtgttaaacg gtaggagaaa acctcttttt tattgatatt tgattttcat
    19261 caaacagtca catagtcgat tgcttctgct tgcggaatcg tttcatctac aaagtagact
    19321 ttataccatg tcaagaaact gaagattgtc cagatttttc tttgctcttc tttacgtcct
    19381 tcgtgatggt catctagcaa cttcatgatt ttttcttgat caaaaaattc ttttgcaaaa
    19441 tctgcttcga ataatgcttt gacttgttcg tagccgtgtt cttctttcaa ccaagctttg
    19501 attggcacag ggaatcctaa tttcacacga ttcgaccatt cttccggaag atgtttgttc
    19561 gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca ataaaaactt acttggtatt
    19621 tgttgagcca atttcatgac ttcgatatcc aataaaggta ctcggacttc caaagaactt
    19681 gccatagaaa gtttatctgc ctttaacaag atatcttttg gcatccattg atgaagatcg
    19741 acatattgca tcttattgac ttcatctttg attccttctg ttttctcgta atgaacagtc
    19801 atgatttcat ccacagatgg tgctgtttga tattttggtg ttaacacttc tgatgcttct
    19861 gattcttcga acactcgagc atgaccgata aaatattctt ttgcaggagc aagtgattca
    19921 tataaatgga tccgtccatg gaagtttttc atttttccga ttttacgtcc taaattatag
    19981 cgtgttcctt ttggtaattt tttcaatcct tgagcaatca cacggatgaa ttttgaattc
    20041 gtatggaatc catacgtttg gtatcccgca aacagttcat ccgccccttc tccagattgg
    20101 actacccgca cactttgtga agcaagattt gctaaaaagt acaaaggtac gcaagaagga
    20161 tttgaatcag gttcatctaa gtgatactgg atcaaaggga atgctttgaa cgacatgtct
    20221 ccgtcgatca ctgctgctgt attatctaag tccaatagtt cggttagttt tctggcttca
    20281 atcgcttcat tataggtttt atcatcaaac ccgattgaga aagaatgatc tggtttcaat
    20341 acagaggtta cataactgga atcaaccccg ctggataaga aagaaccaac ttctacatca
    20401 ctgaccgtat gtgcttcaat agaagcttga acggtttcgt cgattttttc gatccattct
    20461 tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc aatattgctg aatgtctatt
    20521 tttccatctt tatacgtata ataatggcct tcaggcaaac gatagacatc tttgaagaat
    20581 gtttcgccgt ttaatggaga atattggaaa gtcatataag gtttcaacgc ttctttgttc
    20641 aattctttgt taaaatcagg atgaggcaga aaacttttga tttctgaacc aaacataaat
    20701 gttccgttca tttctgcata ataatatggt ttgatcccaa agtggtctct tgcaccgaat
    20761 aactcattct tttcattatc ccaaatagca aaagcaaaca ttccgcggat cttttgcaat
    20821 aattctgttc cccattcttc gtaaccatgc aaaaggactt ctgtatctgc atgtgtttga
    20881 aaaacgtggc cagctgcaat caattcttct ctcaaaggct ggtaattata gatttcgcca
    20941 ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag gctgagtacc gccctctaag
    21001 tcaataatac ttaatcttct gaatcctaaa gcaacatgtt tatcgatata ttctcctgaa
    21061 ctattaggtc cacggtgaac gattcgatcc atcattgtat taataattgt ttttttgttg
    21121 tccttgtcgt taacaaaacc tacaataccg cacatttaac tttcacatcc taatataaaa
    21181 ttactgttta tttcattcat atgcatctta tcatagaata aatagcggac gaatattttc
    21241 tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt ttctgttttt acagaaaata
    21301 aagtcccaat atctaattta ccatgttttc ttttttgagg ctaccttatt ctcttcgtgt
    21361 taaaaaaata ttagtggatg ctatttccaa aaaaaccagc aaaaaaagac acgacctaaa
    21421 gatcgtgt
//
LOCUS       DAZKOR010000048        20588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000048 DAZKOR010000000
VERSION     DAZKOR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.048"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG8ZM_001667"
     CDS             352..1041
                     /locus_tag="ACG8ZM_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0492626.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG8ZM_001668"
     CDS             1028..2236
                     /locus_tag="ACG8ZM_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0492627.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG8ZM_001669"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG8ZM_001669"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0492628.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8ZM_001670"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8ZM_001670"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0492629.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACG8ZM_001671"
     CDS             complement(6339..7034)
                     /locus_tag="ACG8ZM_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0492630.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8ZM_001672"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8ZM_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0492631.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACG8ZM_001673"
     CDS             complement(8233..8424)
                     /locus_tag="ACG8ZM_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0492632.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACG8ZM_001674"
     CDS             complement(8445..9335)
                     /locus_tag="ACG8ZM_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0492633.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACG8ZM_001675"
     CDS             complement(9322..10089)
                     /locus_tag="ACG8ZM_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0492634.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8ZM_001676"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8ZM_001676"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0492635.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8ZM_001677"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8ZM_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0492636.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8ZM_001678"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8ZM_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0492637.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACG8ZM_001679"
     CDS             14660..15043
                     /locus_tag="ACG8ZM_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492638.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8ZM_001680"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8ZM_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0492639.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACG8ZM_001681"
     CDS             complement(15987..16805)
                     /locus_tag="ACG8ZM_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0492640.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8ZM_001682"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8ZM_001682"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0492641.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8ZM_001683"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8ZM_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0492642.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17934..19268
                     /gene="dnaA"
                     /locus_tag="ACG8ZM_001684"
     CDS             17934..19268
                     /gene="dnaA"
                     /locus_tag="ACG8ZM_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0492643.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19466..>20588
                     /gene="dnaN"
                     /locus_tag="ACG8ZM_001685"
     CDS             19466..>20588
                     /gene="dnaN"
                     /locus_tag="ACG8ZM_001685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0492644.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVR"
BASE COUNT         5878 a         4206 c         3789 g         6715 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgcggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggagtcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa atggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggtgattt cctgtgtatt ccgtctccta actattggta tatctatatt
    17701 ttttatccac agatgcaaga ggaaatctat tgatttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt tttgacaact gtgtttttct cttttttctt tttgtcgatt
    17821 gtgtggataa ttgtaattaa aaaatttttt tcttcggttt ctgtggaaaa ctattgaaaa
    17881 aaatgctaaa ttagtactag ttattcactt tttaatgaag gaggaggcct tatatggtat
    17941 ccctcgatgc tttatggaat gaattaaaag caacatacca aaaggattta tcgcctgcta
    18001 gctataatac atggatcgaa acagcgaacc ctcgaaccct tgatcaaaac cagctcgttg
    18061 ttgaggttcc tagcaagatt cataaagaat attgggaaaa aaacctggcg acaaaaatcg
    18121 ttgaagtcgg ttatatgtta tcaggaaatg agattatccc tcgctttatt actggcgaag
    18181 aagcagagca agaagaagtt atagaagaaa aaaatccaaa agttgtggca cctagtccgc
    18241 taaaaaaagc catgctgaac cctaaatata cctttgatac gtttgtcatt ggtaaaggaa
    18301 accagatggc tcatgcagca gcgctcgttg tagcagaaga tccaggttct atttataacc
    18361 cgctcttctt ttatggaggc gtgggacttg gtaagactca cttgatgcat gcaattggtc
    18421 atcaaatgct gcaaagtcag cctaatgcca aagtaaaata tgtaagcagt gaaacttttg
    18481 ctaatgattt catcaattcg atccaaaaca aaacagcaga agagttccgt caagaatatc
    18541 gaaatgttga tttactgcta gtggatgaca ttcaattttt tgcagaaaaa gaagctacgc
    18601 aagaagagtt tttccatact ttcaatgcct tgtacaatga agggaaacaa atcgtactaa
    18661 ccagtgatcg gttgccaaac gagattccga aactgcaaga acgcctcgtt tctcgctttg
    18721 catgggggct gtctgttgat attacaccgc cggatcttga aacaagaaca gcgattttac
    18781 gtaaaaaagc ggcggctgaa cgtctagaga tcccagatga cacactaagc tacatcgcag
    18841 gacaaattga ttctaacatt cgtgaattag aaggtgcgct tgttcgagtg caagcttttg
    18901 ctgcgatgaa tagtgaagat atttcgacta gtctagctgc agacgcactg aaaacgttaa
    18961 aatcaggaaa aggacatcct caattgtcaa tcctgcagat ccaagaagaa gtggcaaagt
    19021 attatcacat ccaattgaaa gatatcaaag gaaagaaacg cgtcaaatct attgttgttc
    19081 ctagacaaat cgctatgtat ttggcacgtg agctcacaga taattcttta ccgaaaatcg
    19141 gagctgaatt cggtggcaag gatcatacaa cggttatcca tgctcatgaa aaaatccagc
    19201 agctgatgga ttctagtgtc agtatgcaaa atgaagtatc agaaatcaaa aactttttac
    19261 taaattaatt tgtcgattct atcaactgaa aatagcatgt ggataaaaga aaaaaacgtt
    19321 caaaagttat gcacactttt tccacagctg ttttttcgtt tctctcttac gttttttgag
    19381 ttttccacag aatcaacatg ccctattact tttattattc tttttaacta ataataaata
    19441 aacaaacatt caaaggagat acatcatgaa agttacttta aaccgagcta gctttatgca
    19501 ggaattgcaa actgttcaac gagctatttc aagcaaaacc acgatcccta ttttgacagg
    19561 tgtaaaaatc acactgacac aagaaggttt gactttgacg gggagcaacg ctgatatatc
    19621 aattgaaact tttttgtctg ttgaaaacga aaaagcaaat atgcaaatcg aatctactgg
    19681 ttccattgtt ttacaagcac gtttctttag cgaaatcatt cggagacttc ctgaagaaac
    19741 atttacttta gaagttttag aaaataaaca agtagcgatt acttctggaa aagcgaattt
    19801 tatcgtaaat ggattagatg cagataacta tcctcatctt cctgttgtcg aaagccataa
    19861 ccagatgaaa ttacctgtac acgtattgac taaactaatc aacgaaacag tttttgctgt
    19921 ctcacaacat gagagtcgtc caatcttgac aggtgtgcat ttcattttat ctgataattc
    19981 tttattggct gtagctactg attctcaccg tctaagtcaa cgcgtgattc cagtagaaca
    20041 agcggctgat cattttgata ttgttattcc tggaaaaagt ttgatcgaat tatctcgctc
    20101 attaacaaat gaagaagaaa tcgtcgaaat cagcattatg gaaaaccaag tgttgttcaa
    20161 aacagaaacg atgtatttct attcccgttt gctagaagga aactatcctg ataccaatcg
    20221 tttgattcct tcaagtttta atacagaggt tgaattttct gttcctagct ttttagcggc
    20281 gatcgaacgg gcttctttac tttctcatga aggccgtaac aacatcgttc gtttatctat
    20341 tcgtccagat gcggttgttt tatacggaaa ttcgccagaa atcgggaaag tagaagaaag
    20401 cttgagttat acagcaagca gtggcgatcc attagatatt tctttcaatc cggattatat
    20461 gaaagcagcg ttgcgtgcat ttggagatat gagcatcaaa gtgaaattta tctctgctat
    20521 tcgtccattt acattggaac caacggaaga tggcgttcaa tttattcagc tgattactcc
    20581 tgtacgta
//
LOCUS       DAZKOR010000049        20184 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000049 DAZKOR010000000
VERSION     DAZKOR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.049"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="ACG8ZM_001686"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="ACG8ZM_001686"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0492645.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="ACG8ZM_001687"
     CDS             1013..2260
                     /locus_tag="ACG8ZM_001687"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0492646.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="ACG8ZM_001688"
     CDS             2336..2680
                     /locus_tag="ACG8ZM_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492647.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="ACG8ZM_001689"
     CDS             complement(2797..3123)
                     /locus_tag="ACG8ZM_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492648.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4698)
                     /locus_tag="ACG8ZM_001690"
     CDS             complement(3496..4698)
                     /locus_tag="ACG8ZM_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0492649.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4815..5330
                     /locus_tag="ACG8ZM_001691"
     CDS             4815..5330
                     /locus_tag="ACG8ZM_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0492650.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5529..5975)
                     /locus_tag="ACG8ZM_001692"
     CDS             complement(5529..5975)
                     /locus_tag="ACG8ZM_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0492651.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="ACG8ZM_001693"
     CDS             complement(6022..7500)
                     /gene="msr(C)"
                     /locus_tag="ACG8ZM_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0492652.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7854..8483)
                     /locus_tag="ACG8ZM_001694"
     CDS             complement(7854..8483)
                     /locus_tag="ACG8ZM_001694"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0492653.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8467..8931)
                     /locus_tag="ACG8ZM_001695"
     CDS             complement(8467..8931)
                     /locus_tag="ACG8ZM_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0492654.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9418..10686)
                     /locus_tag="ACG8ZM_001696"
     CDS             complement(9418..10686)
                     /locus_tag="ACG8ZM_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0492655.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10917..11654)
                     /locus_tag="ACG8ZM_001697"
     CDS             complement(10917..11654)
                     /locus_tag="ACG8ZM_001697"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0492656.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11721..12290)
                     /locus_tag="ACG8ZM_001698"
     CDS             complement(11721..12290)
                     /locus_tag="ACG8ZM_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0492657.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12418..13290
                     /locus_tag="ACG8ZM_001699"
     CDS             12418..13290
                     /locus_tag="ACG8ZM_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0492658.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13632..14504
                     /locus_tag="ACG8ZM_001700"
     CDS             13632..14504
                     /locus_tag="ACG8ZM_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0492659.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14501..16459
                     /gene="nagE"
                     /locus_tag="ACG8ZM_001701"
     CDS             14501..16459
                     /gene="nagE"
                     /locus_tag="ACG8ZM_001701"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0492660.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16710..17561)
                     /locus_tag="ACG8ZM_001702"
     CDS             complement(16710..17561)
                     /locus_tag="ACG8ZM_001702"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0492661.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17684..18151
                     /locus_tag="ACG8ZM_001703"
     CDS             17684..18151
                     /locus_tag="ACG8ZM_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0492662.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18414..18896)
                     /locus_tag="ACG8ZM_001704"
     CDS             complement(18414..18896)
                     /locus_tag="ACG8ZM_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0492663.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18996..19859)
                     /locus_tag="ACG8ZM_001705"
     CDS             complement(18996..19859)
                     /locus_tag="ACG8ZM_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0492664.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6068 a         4157 c         3663 g         6296 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tacaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4441 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4501 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4561 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4621 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4681 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4741 taaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     4801 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     4861 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     4921 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     4981 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5041 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5101 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5161 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5221 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5281 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5341 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5401 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5461 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5521 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5581 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5641 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5701 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     5761 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     5821 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     5881 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     5941 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6001 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6061 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6121 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6181 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6241 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6301 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6361 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6421 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6481 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6541 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6601 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6661 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6721 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     6781 cggtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     6841 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     6901 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     6961 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7021 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7081 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7141 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7201 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7261 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7321 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7381 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7441 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7501 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7561 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7621 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7681 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7741 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     7801 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     7861 aacgtagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     7921 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     7981 aactgatctg cttcgactcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8041 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8101 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8161 ggcatcttcc ctgattcgta agcttggtta tagatttctt cgccatattc gtaacattgc
     8221 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8281 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8341 tgcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8401 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8461 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8521 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8581 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct cgattttctt
     8641 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8701 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     8761 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     8821 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     8881 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     8941 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9001 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9061 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9121 tattgattct tatcaaacta tagaagatag ctcttctgca gtagaaaatt ttacaccgcc
     9181 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9241 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9301 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9361 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9421 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9481 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9541 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9601 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9661 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9721 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
     9781 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
     9841 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
     9901 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
     9961 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10021 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10081 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10141 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10201 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10261 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10321 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10381 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10441 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10501 aattgtattt tctgagacag tagctgactg caaagtactc tctgtatcgt tttcaattcc
    10561 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10621 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10681 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10741 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttga taaatttgct
    10801 ttttctacat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    10861 ggtcgtttga ataactattt aacaagtaat tctctttgga tattatccgt tatggattat
    10921 acttcctgtg tcgtcggacg gataatcatg ttactgacag acatccggtc tggcgtttct
    10981 atcgcaaatg ctacagcaga agctatatct tcggaggtca atccccattc tttttgcgct
    11041 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11101 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11161 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11221 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11281 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11341 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11401 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11461 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11521 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11581 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11641 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11701 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    11761 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    11821 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    11881 tttttcttga tgtggattgg ctttactaac aagcctcttc gttcttttgg atcggttaga
    11941 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12001 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12061 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12121 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12181 gctgctaaag gctcttcgtt ttcagcttgg atctgatgga tcacttcaaa gagcggatcc
    12241 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12301 ccttttatcc ttactttata acggacggct tatcatgtct aatacttata aaacatactt
    12361 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12421 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12481 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12541 gaacttggtg tgacactttt cactcggggc tcccgagaga tacagttgac ggattctggt
    12601 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12661 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12721 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    12781 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    12841 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    12901 caagatcgtt ggggactttt gatacatcga aatcatcctt tagcaaagca aaccgtcatc
    12961 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13021 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13081 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13141 gggatcatca atacttatgg gacggaaaaa atattcatcc cactcgatcc gccattacaa
    13201 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13261 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13321 ttaacaacga ataagataag caaaagatcc aacgattaaa taacataagt ttcttaattt
    13381 atttcttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13441 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13501 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13561 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13621 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13681 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13741 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    13801 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    13861 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    13921 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    13981 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14041 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14101 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14161 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14221 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14281 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14341 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14401 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14461 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14521 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14581 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14641 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14701 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    14761 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    14821 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    14881 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    14941 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15001 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15061 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15121 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15181 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15241 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15301 aaacaaagca aaaacagctt ccttgatgct agcagcagct tttgcttcat tctttactgg
    15361 tgtgacagaa ccacttgaat tttcatttat gttcgttgct tggccattat atgtcttgca
    15421 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15481 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15541 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15601 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15661 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15721 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    15781 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    15841 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    15901 cgttgcagat gagatggcaa aactacatgg tggtgctgca gcacgacctg cacaaacaaa
    15961 caccgtggtt aaaacagaaa catttgccgg tgaaacgatc gaacaagata tttacgctat
    16021 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16081 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16141 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16201 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16261 atatgtagaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16321 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16381 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16441 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16501 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16561 caactccgcg gtaataagtc cagctaaacg aaacatatga agagctattt tttatttgac
    16621 tcttcttatt acttgatgca gaacagcttt ttcacaaaat ttttcttcat actcaaaact
    16681 aatcggcttt ttcacaacct ctttatacat tattcctgat ccaatatttc ttttaatgct
    16741 gcttcaagag aagttaatgg ttttcccaat actttttcta aatcatcaga ggaaatagct
    16801 aactgatcct ttttaatatc atgttggatc gctaaaaaca tgtcagctgc ttcttgaggg
    16861 aatccggctt cttttagatt ttcgacaaac gctgcatcac ttgcttcaat aatttctaac
    16921 tctttacctg tcgcacgctc taatgccttg gctaattctt catagtcagt tagattgccg
    16981 gataattcta agatttctgg agagtcactt tctactagta cttttgctgc agcttctgca
    17041 tattcacgtt tcaatgccca acctgttttt ccatttcctg cagcataaac gaattgacca
    17101 gtttttaaag cctgcccaat gataggtaat tcattctcta cataccaatt attccgtaaa
    17161 aatgtatggg aaatacctga atccttaatc attttttcag tgaattggtg atctggcgct
    17221 aatacactga tcgagtgatc ggcatctgca aaacttgtat aagcgatata agatacccca
    17281 gctgcttgtg ctgccttcac tacattctta tgttcggctt gacgatttcc tggaacgcca
    17341 gagacaaaaa gcaatcgatc tattcctttc aacgcttctt tcattgagtc taaatcatca
    17401 taatcaccaa tacgtatatt aaatccagct tctttcagtg cttctccttt ttcttcacta
    17461 cgtactagta catagatttc attttgtgga accatctttt ttaagaattc cagcgcataa
    17521 ccaccaaaac ctccagttgc tcctgttact aaataatgca tatttgccac tccctattct
    17581 ttttatttgt taagatttac agctgtacat ttataaatta cacctttatc ataaagagtg
    17641 tggtaagata agtcaagcaa aaaaaatgaa aggatgacaa ctgatgaagt attctctgca
    17701 atttagtgat gcaatccatg tacttgccta tattgaaatg tttcaagata cccgatcact
    17761 ttctagtgag atgattgcca gtagtatcga aacaaatgca gcaaatgtac gtaaaatcat
    17821 gagtcagctc aaaaaatccg gtttgatctt tacaaaaacc ggaaaaccgc agccttccct
    17881 tgccaaaagt ccagatcaga tttctttact agatgtctat cgcagcatcg aaggtaatac
    17941 acagcttatc caagtcgatc caaaaacaaa tccaaattgc gtcgttggtg cgaatatcca
    18001 agaagcttta gaagaaagct acgctcggct gcagaaaaag gcagaagaag aaatggctaa
    18061 gatcacactt gatagtctga tacataaaat agccgttttg gaaaaagaaa aaagaccaga
    18121 aaacacaggc ctcgtagaga gatttttata attctctcta ctgtgcctgc atttttctga
    18181 tcggaatcgt ctacataaat tcttttattc tccgggggac aaaagccttg tcatgcccct
    18241 ttttcctctc taatgctcag gtcaaagcgc tgaagtcatg acgtcttgat aaacggtgtt
    18301 caagtagctg accctcggca ttagttctga aaaatgcaaa atatggaaag ctatttccca
    18361 tttttcctct ctaatgctca ggtcaaagcg ctactttcac gacctctata ttatcatggt
    18421 ttattgacta ccggtatcca gatttctccc atatatttcc cgttggtaac tcccatttcg
    18481 actacagcat ttggaccccc aacataggcg taatcttcta cttctcttaa aacatgccca
    18541 aaggccgtat tcgtcaaatc atttcttaat tcttcaggcg attctgctaa agcgcttaca
    18601 acaagatacg ttccttttgg gaatgggatg attcgcgtag tgtttggaac atcctgactc
    18661 gtttgaacac cgacatagta catcattttc ccgttatagg gttcgttgac tgtaaagaaa
    18721 taatgatttt cagcagcttg tttcaatgca tctattgttc cattaccgat aactttttgc
    18781 tcaaatgctt gtttctctgc agccaaaccg acaaaatccg tattgctact cttcaattcc
    18841 acaccaatac ccgtcaccgt aaaatctggt ttttcttcga tcctatactt tgccatttct
    18901 cccacccttt ctattatatt aagttcatct tacataaaat taaagggaaa tgacacgatt
    18961 acgctctttt ttctagggat atttttattt tttaactatt tttttgcttt cactgcaatc
    19021 caaatctcac tgtaaacatt actgaaatcc tctccccatt ttgtaaaaga gatctcggga
    19081 gcttcgacta acccataatc tgaagaagga agccattctg catagatttt cccccaagta
    19141 tcttgtaaag tttggggaaa tggcccttta ttaggaaaaa tggcccaagt gtgttcttct
    19201 atagcaagca tttctaaatc agcgtacgga ttttcttttg tcgtagcaaa gccaatcatg
    19261 tgcatcaaac tgcccttttc ttccaagcgg tctccatcaa atccataaga aacgttgatt
    19321 acttgatttg gatataaatc tcctaactta tgcatctctt ccctttgagt ctccgtgatc
    19381 gtctgcgcta acttttgtat ttcttgattg actccttcaa atttgacagg aacttttttc
    19441 gccacacctg ccacataaaa ctgttctttc ttttcgatac gaaattccat cgattttcct
    19501 cctctgacat caataaaaaa tgtcaagcgt ggaaaagatt tttgtatgcc gtatctgctg
    19561 atttctgaag gcagatatcc tgaccaatca cgaaaagctc ttgaaaaacc ttcgactgtt
    19621 tcatatccat atctaaacgc cgtttctgtc actcttgatc catctagaag atcgcgatta
    19681 gccaaagcca atctacggtt tttgatatag tccattagcg acatccctgc aataaacgta
    19741 aagatgcgtt ttaattgata ttcagagatt cctgtatttt cggctattat tttataggaa
    19801 agtccttctg tcaaatgttc ttcgatgtaa tcgatcaatt ggttcagatc tttaagcata
    19861 tttatcttcc tccttgattg cctttttatc atattatcaa agaaagtcaa ttacttgatg
    19921 aatcaggaac caatttagtc agatgtagaa aatgttgtat ggatctaaag aaaattctca
    19981 agcagcgttt cgtaacggat aattggtatt atctgcctat ctatgtttta cctttagaaa
    20041 aataaatttt ggggaagaca gattctaagt aaacaaagcg gtaaaaatat cttttattcc
    20101 cttagttaat ccgcttacta cctactgtac aaagcaaatt gttgttcaaa ctgctgatca
    20161 gaacgctgct tggatttttc tttg
//
LOCUS       DAZKOR010000050        20140 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000050 DAZKOR010000000
VERSION     DAZKOR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.050"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1648)
                     /locus_tag="ACG8ZM_001706"
     CDS             complement(89..1648)
                     /locus_tag="ACG8ZM_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0492665.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1803..4037
                     /gene="pcrA"
                     /locus_tag="ACG8ZM_001707"
     CDS             1803..4037
                     /gene="pcrA"
                     /locus_tag="ACG8ZM_001707"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0492666.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4054..6090
                     /gene="ligA"
                     /locus_tag="ACG8ZM_001708"
     CDS             4054..6090
                     /gene="ligA"
                     /locus_tag="ACG8ZM_001708"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0492667.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6196..6501
                     /gene="gatC"
                     /locus_tag="ACG8ZM_001709"
     CDS             6196..6501
                     /gene="gatC"
                     /locus_tag="ACG8ZM_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0492668.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6501..7973
                     /gene="gatA"
                     /locus_tag="ACG8ZM_001710"
     CDS             6501..7973
                     /gene="gatA"
                     /locus_tag="ACG8ZM_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0492669.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7970..9400
                     /gene="gatB"
                     /locus_tag="ACG8ZM_001711"
     CDS             7970..9400
                     /gene="gatB"
                     /locus_tag="ACG8ZM_001711"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0492670.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9410..10465
                     /locus_tag="ACG8ZM_001712"
     CDS             9410..10465
                     /locus_tag="ACG8ZM_001712"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0492671.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10576..11949
                     /gene="rlmD"
                     /locus_tag="ACG8ZM_001713"
     CDS             10576..11949
                     /gene="rlmD"
                     /locus_tag="ACG8ZM_001713"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0492672.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11952..12080
                     /locus_tag="ACG8ZM_001714"
     CDS             11952..12080
                     /locus_tag="ACG8ZM_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0492673.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12520..12708
                     /locus_tag="ACG8ZM_001715"
     CDS             12520..12708
                     /locus_tag="ACG8ZM_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0492674.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12786..13799
                     /locus_tag="ACG8ZM_001716"
     CDS             12786..13799
                     /locus_tag="ACG8ZM_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0492675.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13866..14900)
                     /locus_tag="ACG8ZM_001717"
     CDS             complement(13866..14900)
                     /locus_tag="ACG8ZM_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0492676.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            15015..17240
                     /locus_tag="ACG8ZM_001718"
     CDS             15015..17240
                     /locus_tag="ACG8ZM_001718"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0492677.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17258..19141
                     /locus_tag="ACG8ZM_001719"
     CDS             17258..19141
                     /locus_tag="ACG8ZM_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0492678.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19122..20096
                     /locus_tag="ACG8ZM_001720"
     CDS             19122..20096
                     /locus_tag="ACG8ZM_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0492679.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6778 a         3702 c         4327 g         5333 t
ORIGIN      
        1 ttttcccagc ctcttctttt tttatagaat catatagaat caagtagtgt agatgaatag
       61 ttgttgtaaa atagtttttc catgttcgtt aaaatgattg cttcaaattt gtgatagaag
      121 atttagcctc gatttcctgc ttgattcttg gcaagtttgc ttcatcagtc aagataacca
      181 aaatatctcc tacattcatg atcgtatcac cgtgagtcaa gatttcctga tttcctctgc
      241 gtatagagat tagcagcata tttttaggcc aattgaagtc tcgtaccatt aatccgtcta
      301 aagaactctc tgccgttact ggaaaatcaa aagttgtcct tgcacctttt agtgaggcgg
      361 tctgagtatt tccaagtaga cgttccaata atgattcgta aattggatta ccgccgagaa
      421 ggtcattgac gatataagca acaagagcag ccaatcctaa aggcatcaga tgattaagag
      481 agccaaccat ttctgtcact aaaataattg ctgtcagcgg tgcttttcca atcgctgtga
      541 aataaccagc catggcaata acgacaaaat ccttgatgta gattggatcc atcccgaaat
      601 caaccataat gtttccataa aagctaccga ttaaagcccc caatgtcaat atcggaagga
      661 aaatcccccc tggaagaccc ccaccgtaag aaatcatgga aaaaacgaat cgaagaacaa
      721 acaaaccgat aatcataatg attggcaagg aagcatgtcc aaaagctaaa acgatttgat
      781 tgcctccgcc tagagtacta ggccataaat aaccaattgg caaaatcagc aagaaaggaa
      841 cgaacccgtg aaaataagct ggtaaaggca atcggttgta aaattttggc aaagccagca
      901 agacattctg ataaataaaa cctaagatgc ctagaagtac tcctaagatc actaaggaac
      961 cataatattt caacggcaaa gaaggaaggt cacctagata taaaacaggt ctcaagccga
     1021 aaaagtaaag agagacgaag ttagcagtga ttgctgaagc aaatgatgtc agccaaacaa
     1081 gtggagaaaa ggagtgatgg atttcttcta aaacaaagag taatcctgcg ataggagcat
     1141 tgaatgctgc tgcaagtccg ctacttgcac cgctagaaat taaaatcttt tcttctgaag
     1201 atggactccg aaacaattgt ccagctcctt gtccaatgga tgcgccaagc tgtatagaag
     1261 gtccttctct acctagaaaa aggcccgcac caactgaaag tacaccgcca atgaactttt
     1321 tccataaaac agagaaccag ttcattgata attccccgcg aagctgtccc tctacttgtg
     1381 gaatcccgct tcccttgata tccggttcat ttttgaccag ataacccacg aaaaatgctg
     1441 caaggatgca gcaaatagct aggccaatta agtaaatcgg ctgttcccga ctcatttcat
     1501 agatacttcg aactaaatcg cttagctttt cgatagataa tcgaaacagg ctaacgacga
     1561 ttcctgtcag cagtccaaca aatattcctt ttagaataaa atatatctga gtcttatcta
     1621 atttcttgat ttttcctttt ttttccattc aaacgcctcg ctttctttta atttagcgcg
     1681 aaatcaacga attgacaagg aaaaatccat aaaaagaaac aaatgatttt gaactagcta
     1741 gtttaatgaa agccaagaaa atgctataat taacaagatt gataaaagaa aggatgttgt
     1801 taatgtcatc caaagctgaa ttattaaacg ggatgaaccc ccgtcagaaa gaagctgttc
     1861 ttcacactga cggaccatta ctgctaatgg caggtgcagg aagtggaaag acgagagtgc
     1921 tgacacaccg cattgcctat ttgattgagg aaaaagaagt caatccttgg aacatcctag
     1981 cgattacttt taccaataag gctgcaaaag aaatgaaaga acgtgtcaat gcgattctcg
     2041 catctggagg cgaagatgtc tgggtatcga cttttcactc gatgtgtgtg cgtattttaa
     2101 gaagagatgt tgattttatc gggtataacc ggaactttac aatcatcgat tcttctgaac
     2161 agctgacctt gatgaaacgg atactaaaag aactgaacat tgatccgaaa aaatatgatc
     2221 ctcgaagtat attaggaaca atctctcaag caaaaaacag cctgcagacc cctcaggatt
     2281 ttgcaaaaat gcaaggcagt tattacgaag agattgcggc aaaatgttat gctgcttacc
     2341 aaaaagagct tcaatataat caatgtatgg actttgacga cctgatcatg aatacgattc
     2401 gattatttga agaacatcct gattcactca cgtattatca aaacaaattc cattatattc
     2461 atgtagatga gtaccaagat acaaatcacg cacagtatac gctagtcaat ttattggcag
     2521 gtagatttag gaacctgtgc gtcgtgggag atgcagatca aagtatttac ggctggcgcg
     2581 gtgcagatat gcagaacatc ttggattttg aaaaagatta tccagatgct gcagtaattt
     2641 tgctagaaca aaactatcgc tccacgaaaa acatccttag cgcagcaaac caagtcattg
     2701 aaaacaatag caatcgaaaa ccaaaaaact tgtggacaga aaacaaagaa ggaaacaaga
     2761 tcacttacta ccgtgcagat aatgaacgag acgaaacccg ttttatcgta gaccggatgc
     2821 aagaagagat tcgaagcaat catcggaatt acggcgactt tgccatcttg taccggacga
     2881 acgcacaatc tcgtgtaatg gaagaaactc tgttgaaagc aaatattcct tataaaatgg
     2941 ttggagggca caagttctac gatcgtaaag aaatcaaaga tattttagcg tatttgaatg
     3001 tgctggtaaa tcctcaagat tcaatcagtt ttgagcgtat cgttaattcg cccaaacgag
     3061 gcatcggacc aggatctatc gaaaaactac gcagttttgc ttctcttcat gaatggccac
     3121 tgcttgaagc agcgcaaaat gtggatctgg caaatatcgg tggaaaagca ggacagcaat
     3181 taggtgcatt tggagagatg atccaagaag tcactcagat gatcccatac ttgacagtga
     3241 cagaattgac gaaagaggtt ttggatcgta gcggatatct agaagactta aaaatccaaa
     3301 atacattaga agcacaagca cgcatcgaaa acttggagga atttttaacg gtcacacaag
     3361 aattcgacaa acaattcgaa cagcaaaatg aagaagacgc tgatgcacca gaagaaaaac
     3421 taacggtctt cttaaatgat ctggcacttg tttccgacat tgacaattta gaagaagatg
     3481 cttcccaagt gacattgatg actttgcatg cagccaaagg attagaattt ccagttgtat
     3541 tcctgattgg tctagaagag ggggtttttc ctttatctcg agcattgatg gaagaaagcg
     3601 aactggagga agaacgccgt ctcgcatatg tggggatcac gcgagcagaa gaagcgttgt
     3661 atttaacaaa tgctttctcc agaacattat atggacggac gcaatataac cgaccttctc
     3721 gttttgtaga agaaatcgat caggagttac tggaaatcga aggcatgcgt cctacgccta
     3781 aaaaaacgcc tgtattcgct aaaaaaacag catatagcta taaacagcca gagacagcgg
     3841 ttgttccctc taaaagtgct actggagggg aaaataacag ctggaaacct ggagataaag
     3901 tcaaacacaa aaaatggggt ctaggaacag tcgtccgagt aagcgggacg tcgaaggatc
     3961 tagaactaga tgtggctttc ccaagtcaag gagtaaaacg tcttttggct gcctttgcgc
     4021 caattgaaaa agcttagtag tggagggtaa atgatggaac atgaaatgac gattgacgaa
     4081 gctgctaaac gagcagaaga acttcgtaca agactgaacc aatggtctcg cgaatactac
     4141 gtagaagata aaccaacagt ggaagactat gtttatgata aagaatacgc agaattagta
     4201 gcaatcgagg aacaatatcc tgatttgatc acgtctgatt ctcccactca acgagtaggc
     4261 ggaaaagtat tagaaggttt tgaaaaagtc actcatgata ttccgcttta cagtttgaac
     4321 gacgttttca gcaaagaaga gctgatagct tttgatcaac gtgtacaaaa agcagtggga
     4381 cgtgtagttg actattgctg tgaactaaaa atagatggac tttccgtttc tttgcgttat
     4441 gaagatggaa atttcgtgag aggtgcgact cgcggggatg gaactgtagg agaaaacatc
     4501 actgaaaacc taaaaactgt ccggtcggtc ccaatcaaat taaaagaacc aatgaacatc
     4561 gaagtaagag gcgaatgttt catgccaaaa cggtcatttg tccaattgaa ccaagacaga
     4621 gaagcagagg gaaaagatat ctttgcgaat cctcggaacg ccgcagctgg ttctttgcgt
     4681 cagttagatt ctaaaatcac agcgaaaaga aatctggaca cttttttata cacagtagct
     4741 gatttcggtc ctatgcaagc aaaaacccag tatgatgcgc tagaagagct agaaaaaatt
     4801 ggattccaca cgaatcggga aaaacgtcta tgtcattcga ttgatgaagt gtggtcatac
     4861 attgaagaat atcatgacaa acgtgtggat ttgccctatg aaatcgatgg aatcgtcatc
     4921 aaagtgaatg aattctcgct acaagatcaa cttggtttta ctatcaaagc accgagatgg
     4981 gcagcagctt acaagtttcc gccagaagaa gttgaaacgc taatcgaaaa catcgaatgg
     5041 acagtcggtc gaacaggggt agtaacgcca acagctatca tgacaccagt ccgagtagca
     5101 ggaacgacag taagccgtgc cagcctgcat aacggtgatt atattaagtt gaaggatatc
     5161 cgcttgaaag atacagttct gatttataaa gcgggagaca tcatcccaga agtttcgcaa
     5221 gtcgtgttag ataaacgacc taaagacagc gaagaatacc aattgcctac acattgtcca
     5281 gtttgtggaa gtgaacttgt ccatttggat gaagaagtag ccttacggtg catcaatcct
     5341 aaatgccctg ctcaaatgaa agaaggtttg aaccactttg tttcaagaaa tgcgatgaat
     5401 atcgatgggt tggggccgcg cgtactagaa cagatgtatg ataaaaaact agtagcagat
     5461 gttgctgatc tttacaaatt gacagaagaa gagttattga cgttggacaa aataaaagaa
     5521 aaatcagcaa ataatatttt gacagcaatc gacaacagca aagataattc ggtcgagcgg
     5581 ttgattttcg ggttaggaat ccgacatgtc ggagcgaaag cagcaaaaat tttagcagaa
     5641 catttcggtg atctagaaac attgagtaaa agtgactatg agtcgattat tgcgcttgat
     5701 acgatcggtg atatcattgc cgacagtgta gtcacttatt tcagtaacga agaagtccat
     5761 gaattgatga atgaattaaa acaagcaggc gtcaatttcg aatacaaagg gcttcgaaat
     5821 gcccaattgc aagaagtcga gtctcctttt aaagaaaaaa cagtagtcct tactggtaaa
     5881 ctaacacgat ttactcgtga agaagcaaaa gagacgatcg aaaatcttgg cgggaaagtg
     5941 actggtagcg tatcgaaaaa aacggatatc gtggtagcag gagaagatgc tggcagcaag
     6001 ctgaccaaag cacaagaatt aggaatcgaa gtttggacag aagatcaaat ggcagatgca
     6061 ttggctaaaa gtcgctcagt agaagaatga aaaatgagtt ttttcagaca aatttcaata
     6121 aacaatttca gacacggcac tcttgtgtta aaatagagag caaagttttt aactcgttta
     6181 gaaagaaggg gtagtatggc gattagtgaa gaacaagtga aacacgttgc caaattagca
     6241 aaacttgcgt tcgcagatga agaacttgca agttttaccg accaattagg taaaatcatc
     6301 gatatggttg aaatgctgga agaagtagat acggaaggtg ttccatttac gtcgaatgtg
     6361 gttgaaacag taaacgtgat gcgacaagat cgggcgcaag ctggatggaa ccgcgatgaa
     6421 ttattgaaaa atgtgccgga aacagaagac ggctttatca aagtacccgc aatcatcgat
     6481 aatggggagg ctggcgcata atgacagaat tacacaatcg ttctctagaa gaactgcatc
     6541 atatgcttgt atcgaaagaa atcactgtac aagatttgac aaaaacaaca tttgaacgaa
     6601 tcaaagaaac agagcctgag gtcgaggcat tcattacatt gaacgaagaa aaagcattgg
     6661 aacaggcaaa agctttagat gaaaaaggga ttgatgaaag caacgtcctt gctggtattc
     6721 caattggtat caaggataat atcgtaacaa aagatatctt gacaaccgct gcatctaaga
     6781 tcctttacaa ttttgaacca atttatgatg caacggtcat ggataaaatc tatcaagcag
     6841 atatgatccc tgttggtaag ctaaacatgg atgaatttgc tatgggtgga agtacagaaa
     6901 cttcttattt caagaaaaca aaaaatgctt gggacccaac aaaagtacct ggtggctctt
     6961 ctggtggttc tgcagcagca gttgcagctg gacaaattcc agtttcttta ggtagtgata
     7021 ctgggggaag tatccgccaa ccagcagctt ttaacggcat cgtagggatg aaacctactt
     7081 atggccgtgt ctcacgtttt ggtttgattg cctttgcttc atctcttgac caaatcggac
     7141 caatgacccg tacggtgaaa gataatgcat tagcattgac agctatcagc ggttatgacg
     7201 aaaaagacgg tacttcttca ggcgtttcag ttcctgattt tgcagaggga ctgacaggcg
     7261 acatcaaagg aatgaagatt gctttaccta aagaatactt aggcgaaggt gtggcccctg
     7321 gcgtaaaaga agctgtattg aaagcagcag aaacattccg ttcattaggt gctgttgtgg
     7381 aagaagtcag cttgcctcat tcaaaatacg gtgtggctgt ttattatatc attgcctcat
     7441 cagaagcaag ttccaatttg caacgattcg atggtatccg ctacggttat cgctctgaaa
     7501 atgtgaaatc attagatgat gtatatgtca actcacgttc agaaggattc ggtgaagaag
     7561 taaaacgccg tatcatgtta ggtacattct ccttaagcgc gggttattat gacgcttact
     7621 tcaaaaaagc aggacaagtc cgtacattga tcaaacgaga ttttgaaaaa gtctttgcag
     7681 attatgactt gatcatcggg ccatcttcac caacagttgc attcggaatc ggtgagaaca
     7741 ttaacgatcc aatcacaatg tacatgagtg acatcttaac gatccctgtc aacttggctg
     7801 gacttccagg tatgtctgta ccggcaggtt tatcagaagg attaccagtt ggattgcaga
     7861 tcatcggtaa ttattttgat gaaaaaacga tgtatcaagc ggcatatgcg tttgaacaag
     7921 caacagaatt ccataaacaa caaccagcta ttttaggagg gaaagacgaa tgaattttga
     7981 aacagtcatt ggactagaag tccacgtcga gttaaaaacc aactcaaaaa ttttctctcc
     8041 agcgccagct cattttggtg cagaaccaaa cagcaataca aacgtgatcg actggggtta
     8101 tccaggcgtg ctgccagtaa tgaataaagc tgcgctggaa ttcgggatga aagcagcttt
     8161 ggcattaaac tgtgaaatct ctaaagatac tcattttgac cggaaaaatt acttttaccc
     8221 agataatcca aaagcatatc aaatttccca atttgatcaa ccaatcggtc atgacggatg
     8281 gatcgaaatc gaagtagaag gtaaaaagaa aaaaatccgg atcgaacgtg tccatttaga
     8341 agaagatgct ggaaaaaaca tgcatggtat tggcggttat tcttacgttg atttgaaccg
     8401 acaaggcacg cctttgatcg aaatcgtttc tgaagctgat atgcgttcac cagaagaagc
     8461 atacgcttat ctagaagcat tgcgatcaat tatccaattt acagaagtca gcgatgtgaa
     8521 aatggaagaa ggctctatgc gctgtgatgc caatatttct cttcgccctt atggacaaga
     8581 agagttcggt acgaaagcag aattgaaaaa cttgaactct ttatctttcg tgaaaaaagg
     8641 tttagctttt gaagaaaaac gtcaagcaaa agttcttctt tcaggaggag aaatccaaca
     8701 agaaacacgc cgttttgacg aaacaacgaa taaaacagtt ttgatgcgtg tcaaagaagg
     8761 gtctagtgat tatcgttact tcccagagcc tgatatcccg aaattcgtga tcgatgacga
     8821 atggattcaa aaagtaaaag ctagtttgcc tgagatgcca gcttctcgtc gcgaacgcta
     8881 tatccgtgag tttggacttc cagagtatga cgcaatggta ttgacactta caaaagaaat
     8941 gtctgatttt tttgaagctg ttttggaaaa tggtgcagat gccaagcaag catctaactg
     9001 gttgatgggt gaagtttctg cttacttgaa cagtgagaaa ctggaattag ctgagacaaa
     9061 attgacacct gagaacttag caggaatgat tcgtttgatc gccgatggaa cgatcagctc
     9121 aaaaatcgcc aaaaaagtct tccgtgaatt gatcacaaat ggcggagaag cccaagaagt
     9181 agttgaaaaa aatggttggg tgcaattatc tgatccgaac aaactactgc caatcattaa
     9241 tgaaattttg gataacaacc aacaatctgt cgatgatttc aaaaatggaa aagatcgtgc
     9301 ggttggcttc ctagtcggtc aaatcatgaa agcaactaaa ggacaagcaa atcctggtgt
     9361 cgtgaataag ctacttaaag atgaattagc gaaacgatag aggctgaaaa tgaaaaaagc
     9421 acgcgtaatt tataacccca cctcgggaaa agaattattg aaaaaaaatt tggcggatat
     9481 cctacaggca ttagaagaag caggatttga agccagcgcc tatgcaacta caccagaacc
     9541 agattcagcc aagaatgaag catgtagagt cgcagaacta ggttttgatc tagtagtggc
     9601 agctggtggc gatggcacaa tcaacgaagt ggttaatgga atcgctccgc ttgaacaccg
     9661 tcctaaaatg gccatcatcc cagctggtac aacgaacgac tatgctcgag cattaaaaat
     9721 tcctagagat aatattaaag cagcagcaga agtgataaaa aaacaccaaa ccgtaaaaat
     9781 ggacatcggc ctttctgata acagttactt catcaatatc gctgcaggtg gctatttgac
     9841 agaattgact tatgaagttc catcagaact aaagagtatt tttggttatc ttgcttatct
     9901 tgcgaaagga gcagagatgc tccctcgagt aaagccaatc aaaatgcgct tgaagtatga
     9961 tgaaggggaa tatatcggaa atgcttcgat gtttttccta gggctgacga actctgttgg
    10021 aggattcgaa cagatcgcgc cagatgccaa attggatgat gggaagtttt ctttaatcat
    10081 tgtgaagaca gcaaatatct ttgagatctt gcatattgca gcactcatgc tgaatggcgg
    10141 tcgacatgtc gatgatccaa gaataatcta taccaaaaca agcagtttgt tcgttgaaac
    10201 agaagatgat atcaatcgac cagtaatgat caacttagat ggtgaatacg gcggggaagc
    10261 accgatgcat ttccaaaatc ttcatcaaca cattgagttc tttgccaata ccgatcagat
    10321 tcctgatgaa gcaatcacag gtacagatga agaagaatta gaggttgtca gcaaagaatt
    10381 cgtaaaagaa gtcgaacgtt taacggatga agatatcgac ggtgacggaa aaatagcaga
    10441 cgaaacacct aatcaaaaga attaatcaaa agccggtgct gaacgtagta aggtcagtgc
    10501 cggttttagt tgtgtaatta gctgatttat agtagagttc agtaggattg aaaaataggc
    10561 ttaaaggagc aaaatatgac agaacagcaa atagtaaaga aaaacgaacg atatacagtc
    10621 gaaatcgtcg acttgagtta tgaaggaatg ggggtagcaa aaatcgatgg ttatccatta
    10681 tttatcgaaa atgcactccc tggagaagaa gtagaaatac tagtagtaaa agcaggcaat
    10741 aagtttggat atggaaaagt cgaaacattt attacacaat cacctgatcg tcagcctgta
    10801 gaaaacaatg cattacttcg cataggtatc gcacctttag cccatttgaa gtatgaacaa
    10861 cagcttgtct tcaaacaaaa acaagtagag catgtaatgg cgaagattgc taagatgcca
    10921 gaagttcctg tacttccaac ggtcggaatg gcagaaccat tcggctaccg taataaagca
    10981 cagatccctg ttcgccgtat cgatggacag ttagccaccg gtttttacaa aaagaatagc
    11041 catgatctag tccctatcga agatttttat atccaagatc ctgtcatcga tcaagcaatc
    11101 aaagtagtca gagatatctt gcaacggttc caagtacgag gatataatga agaaaaaaat
    11161 gaaggacagc ttcgtcacat cattatccgt cgtggtcatt attcccatga aatgatggtc
    11221 gttttagtta ctcgtaaaga aaaattcttt aaatccaagg aaattgctga agctattcat
    11281 gaagaattgc cagaagtggt ttcggtcatc caaaatatca atgaagaaaa aacaaatgtg
    11341 attttaggag acaaagaaaa agttttatat ggcagagact atatcgaaga tcagctatta
    11401 ggaaaaacct atcgaatctc agcaaagtca ttttatcaag taaatactca acaagcagag
    11461 tatctgtacc aaacagccat cgagtttgcc gagttagata aaaaagacat tgtgatcgac
    11521 gcttattcag gaataggtac gattggcttg tctttagcag acagagtgga taaggtgtat
    11581 gggatggaag ttattccaga agcaattgaa gatgcacagt tcaatgcttt aaataataaa
    11641 atcgaaaatg ttcattatga agtcggaaaa gcagaaaaag ttatgaaaaa atggcaagaa
    11701 aaagggatca aaccatctgt gatcgtagtt gaccctccac gaaaaggatt agatcatcat
    11761 tttattgaat cagcaatcga aatgtcacca gaacgaatcg tctatatctc atgtaatccg
    11821 gcaacttttg caagagatgc taagttattt gcagaagcag gttatgccgt gaagaaagtt
    11881 cagcctgtag atcttttccc gcaaacccat catatcgaat tagtcgcttc attcgttaaa
    11941 ggagaataaa aatggaccaa aaagaaaaaa tgaaagaatt gatcaaaaag aaacaaggtg
    12001 gaggccgatc caaacaaatg gaaacaccta agaatgatcg taaaaacatg agaaaaggcc
    12061 ctaaaatctt taataaatag acgtgctaaa gaaagactaa gaagtgttaa cttttgttat
    12121 tgtttcttag tctttttagt tagtccacat tattttggcg atttgccaat aaggactgtt
    12181 gagttgtgca aaatcaaatc tgggtcagaa tgaacgtcat tctgtctcag atttttcgtt
    12241 atttcaattt gattaactga ttgatcaaaa agatttttat tttcatattc tcaaatgatt
    12301 caacactaaa tatacatgtt tgacgcggaa attattattc gattttggcg gcatcagggg
    12361 ggatgacgac gtagcagacc cattatttta ctcaagaaaa tgtccgtgcc tattatttag
    12421 aatacgatag tgaacgtgct gggagttttg ttcttttaga gaaagtctct gaagataaat
    12481 tagttgtctt agattttgac tgtaacgaaa aatggcgaaa tggaagagca ccagcaagtg
    12541 attgaccgga tctacgaagc gacacgatat ctgcctttgg atcgtttgtg cttaagtcct
    12601 caatgtggat ttgcttcaac agaagaagaa aatgttttgt ttgaagaaca atggaaaaaa
    12661 tttcttttgt caaaagcatt gcagaagaag tttggggaaa atcattaaat catctaggag
    12721 agcttgatag gaaataacac tatggtataa tcagaatgct taatactaac tactgaggga
    12781 gccttatgaa aaagaaaatt cttaaattta tcggatggtt cttcggaatc attcttggga
    12841 tcgcgtttgc aatctttctt gccttccagc tttcccccag acctggagca ttcttgatca
    12901 accatatgtt ttcaaatact gttcaaatca ctgataaaaa aacgttcgat caagcagaaa
    12961 aaaacgtggc gaaaaaaaca gatttgacgt atgagtccaa gtataaagag aatacttatg
    13021 acatctacta tccaaaaaac agcaaggggc ctgttcctgt tctattttgg gtacatggtg
    13081 gaggattcgt cggcggagat aaatcgggtg tcaaagaatt tgcgacaaaa ttagtttcag
    13141 acgccaacat cgcagtggtt gcgatgaatt atgaattagc gcctgattca gaatacccta
    13201 atcaagttct tcaagtgaat gaactgatca aagatttatt agctgaagaa aaagacgaca
    13261 aattgctaga catggaacaa ttattttttg gtggagatag tgcaggttca caaatcgcat
    13321 tgcaatatgc agctatacaa acgaatgatg cgtatgctaa acaaatgaaa atcaaacagc
    13381 tacttccaaa agatagtctg aaaggaacca tttcttattg cggacctgtt gatttgaagc
    13441 taacagcaca gcagcattcg gatgatcgat ttatgaagtt tttcgtcaaa accgttgcgt
    13501 ggtcgttgat tggaacaaaa gattggaaaa cgagtgagca attgaaagaa gctagtgtgg
    13561 ctgaccatgt ttctaaggaa tttccgccaa cgtatataac tgacggaaat gcatactcat
    13621 tccaggagca aggtcaagca ttagaaaatc ggttgacaga attaaaggta cccgtgcaat
    13681 ccttgtttta tacagataca aaaaaagaga ttacccatga ataccaattt gattacacgc
    13741 taaaagaatc gcaaaattgt taccagcaga cattggattt tgtgaataag tataagtaag
    13801 aggttgtgaa aaagccgttt cgttttgagt atgaagaaac gaagcggctt ttcacaacct
    13861 ctctttcaat acctttttgt tcttgggata aaactttccc taacaatcaa tcgtgcagga
    13921 taagtcaaaa tgattggttc gtcccgtata ccgtcaattc gctccaatgc cattcgaaca
    13981 gctgcttgcc caattgattt cgcattgatt tgaacactgg tcaagccagg ggagaggttt
    14041 gcgattgtct cgatattatc aaagcttgct acaacaatct cttctccaat ttggatattt
    14101 ttttgtttca atgcccgata gacaccaatt gccattggat cgctcccgac aagaatggca
    14161 gtaggaagag gggattcatt ttctaataga ttagtcgcaa gtacctcacc ttgtttttct
    14221 gtccagccac ctagatgata agacatatat ttttctaagt gatgatcgat cataaaatgc
    14281 tgataggcac gcatcctctt ttcatttata gaaatctgct tcccaccgaa ttcatccata
    14341 tcgactttat atcccccgat ataggctatt cttttatgac cattttctaa gaacaattcc
    14401 agtacggttt tagtcatccg ttcaagatct gcatacacca tatcgacctc ttgctggatg
    14461 tcagggttat ccacgacgac tatttgcatg ttttgtttta acaaggcggt aatcgattct
    14521 ttcgtaacag tcccaacaac gatcaccgca cctaactgat ctagatcttc ccattgttta
    14581 ggattatcag atagattata gattcgattc atccctaaat gcagacgtcg agcttcttct
    14641 tccatcccta ctcgcaaatg tttgtaatag atatcctgtt cttcgctcgc tttgccgatc
    14701 gtagagatca caccaatcga aggcattctc tttttgatat atttacgagt actgatgtca
    14761 taatccaacg cagcagctgc ctctactatc cgttgccgag tagtctgagc cacatggatc
    14821 gtctgatcgt tattcaacac acgggaaact gtagcaggag agacatttgc ctttcgtgcg
    14881 acatctctaa ttccaaccat ttttacacac tcctcaaaaa aataaaaaat ttttattgac
    14941 aaaattgttt agtaaacagt atacaggaat tataaaaaaa agtaaacggt ttccgttttg
    15001 ttgggagtga aatgatggat attacgcata gaaaaacaga taaaaatttc atcttatttg
    15061 atgaaaagag ccgagtcttc catttacgaa atacatttct ttcttatctg ataaagatag
    15121 aagaaagcaa tgtattggcg catatttatt ttggcaaacc agtcaagcag tacaaagaca
    15181 ataaaaatta tccccgaaga gatcgtggat tttcaggaaa tgttccctta aatcctgacc
    15241 ggtctctgtc gaaagataca ctaccgcaag aatactcttc tcatggcagc atggactttc
    15301 gaacgcccgc atcgatcatt cagcgcaaga atggatcaga cttactggat ctgagatatg
    15361 acagccatta tattacagat gggaagccag agatcgaagg cttgcctcaa acttatgtga
    15421 tggacaagtc agaggcacaa acgttggtga tttcacttaa ggatagagaa acagcgattt
    15481 atttcgattt gttctatacc atatttactg atagagcagt gatcacacga tctgtaaaga
    15541 taagaaatga aaccggagaa acaatcaaac tagaaaaagc ggctagtttc cagctagact
    15601 ttgcccatac taggcgattt gatgaagtca ttgctttgcc aggagcacat gtcaatgaaa
    15661 gacagatttc acggcaatct gttttatctg gaacaaaagt atttgaaagc cgtcgaggaa
    15721 cgagcagtca ccatatgaat aactttattg ctttagttca tcatcatacg acagaaaata
    15781 ctggagaagc gatcggactt caatttgttt attccggcaa ccattctttc gaacttgaaa
    15841 aagatcagat caatcaattg agagtcgtcg gtgggatcaa cagccatcgt ttttcgtggg
    15901 aattgaatgc aggccagtct tttcaaacac ctgaaatgat cctttcttac tcaagtcagg
    15961 gattgaacaa aatgagtcag atccaccatg agttgctacg tgaaagaatc gcaagaggtc
    16021 gtcatcaatt tgctgaacgg ccgattctgg tcaataactg ggaagccact tactttgact
    16081 tcaatagtga aaaaatcaag gcgatcattg atgaagcaaa ggagctaggc atcgaaatgt
    16141 tcgtattaga tgatggctgg tttggaaaaa gagatgcgga caattcttca ttaggagact
    16201 ggttcgagta tgaaggcaag ttgacaaatg gattacgtga aatagcagat tatgctcata
    16261 gcaaaggatt gaaatttggt ttatggtttg aacctgaaat gatctcggtt gattctgaac
    16321 tgtatcgaac acatcctgat tttcttatgc aggagccagg tagaatgcct tctgcttcca
    16381 gatcacaaca tgtactggat tttacaaggt tagatgttcg gcagacgatt gagaaacaaa
    16441 tgagaaagat tttagatacg attcctcttg actacatcaa atgggatatg aaccgttcgc
    16501 tttcagatgt ctattcgatt acccttgacc cacagagaca aggagaagta gcgcatcgtt
    16561 atatgctcgg gctttatgaa ctacttgaac atttgacaac tgattatccg gaaatcttat
    16621 gggaaggctg ttctggtgga ggtggacggt ttgatgccgg gtttatttat tatatgccgc
    16681 agtcatggac aagtgataac acggatgcag tggagcgtat gaaaatccaa tatggaacaa
    16741 gccttgctta tccgatttcg tccatcacag ctcatgtatc agctgtacca aatcatcaga
    16801 ccgacagatc tacttcgttg aaaacaagag gagaaacagc tatgagcgct gtttttggtt
    16861 atgaactgga tttgacaaag ctgtctcctg aagaaaaaaa gcaagtaaaa gaccaaatta
    16921 tatcctatca aacgattcgt ccggtgatcc agtatggcca ttattatcgt ttggcgagtc
    16981 cttttgaaga aaatattgcc gcatggatgt ttgtgtctcc taaacaagat gaagctattg
    17041 tgtttttagg gaggatacta gcttcagctc aaccagcgtt ccatgaagta tatctgatgg
    17101 ggttagatga tgaggcactt tatcaggaac agacctcgaa gcggatattt tcgggggccg
    17161 aattgatgac agttggactt tacttccccg attttcaagg tgatttccaa acagaactgc
    17221 ttcatttcaa aaagttatga gagagaagga aaaaagtatg aaagcaaata taaaatggtt
    17281 agatgatcca caagttttta gagtgaacca atcaaatgca catagcgatc atgtttgtta
    17341 tgcaacaagg gaagaagtag aaaccacatc ctcccttaaa cagtctttaa atggatcatg
    17401 gcgtttttgt tattcgccaa atccttcggt acgaccaaaa acattttata aaaaagattt
    17461 tgatgcctgt tcttttgatg aaatcactgt tcctagtcat attgaattag caaattatga
    17521 caagatccat tacatcaata cgatgtatcc gtgggaggga catgtatttc gccgtccccc
    17581 tttcacgaca agccctgaga aagaggcgga agggatcttt tcagctgcta aagacaatcc
    17641 ggttggttcg tatttgacga cttttcgatt agaagaaggt ctaaagggaa aacaaatctc
    17701 gattcatttt gctggtgtag aacaagcaat gtttatttgg ttgaatggtc attttatcgg
    17761 gtatgccgaa gattcgttca ctccgtctga atttgattta acaccttatt tggaagaaga
    17821 aaatattcta gctgtggagg tttacaaaag aagtacagct gcattcttgg aagatcagga
    17881 ctttttccga ttttttggga tcttcagaga tgtcttctta gttgctcgag gacaaaacca
    17941 tttagaagat ctttggatca aacctcgctt agatgacaaa tgtcaaaatg gcaaacttga
    18001 agtagatttg tcttttgagc aagtagccaa aaacagtcag ctgaatgtag cgatactaga
    18061 tcctgatggg aaggaaatct atcaacagca atatccgtta aaagagcaaa tgacgattcg
    18121 catcgatgga tttgaaaatg tggcactgtg ggggcatttt cagcccaatt tatataagct
    18181 tgaattggag acgatagacg aagaaaaaag agtaacggag tatattgact attccttcgg
    18241 ttttcgtaaa atcgaaatca aagataaaat catctacttc aatggaaaac ggatgattat
    18301 caacggggta aatcgacatg agtggcatcc gaaaaaagga cgcgccatca ctatagaaga
    18361 tatggaaaaa gatttggcta tcataaaaaa tgcaaagatc aacggcgtac ggacatctca
    18421 ttaccccaac cagatcactt ggtattattt atgcgaccaa gcaggaatcc atgtgatggc
    18481 ggaaacgaac ctcgaatccc atggctcatg gcaaaaaatg ggcgcatttg agccttctta
    18541 caacgtacca ggtgcagtac ctcagtggca ggcggctgtt cttgatcgag caaaaacgaa
    18601 tttcgaaaca ttaaaaaacc atccaagtat tcttttttgg tctcttggaa atgaatctta
    18661 tgcggaagaa aacattcgac aaatgaacga ttacttcaaa aaagtagacc ccgatcgact
    18721 tgtccattat gaagggaatt ttccaaatcc agcattcgaa gattcgattt cagatgtaaa
    18781 aagctgcatg tatgcgccac cagaagagat cattgactac cttgaaaata aagcggaaaa
    18841 accgttcatc ttatgtgaat atatgcatga catggggaat tcgttaggtg gaatggattc
    18901 ttacatgacg ttgcttgatc gttatgaaat gtaccaagga ggctatatat gggattttaa
    18961 agatcaggcg atccaagtga ctgatccaat cacaggcaga gaagtgatgc gctatggcgg
    19021 tgactttgac gataaaccaa gtgactatga attctcaggg aatgggatcg tttttgcaga
    19081 tggacaagaa aaacccgcca tgcaggaggt aagatattat tatgaaaaat acagtaaata
    19141 aaagtcatat ggatacggaa aaagttgcaa tcgtcttcgg cgactgtaca ttaggtgtca
    19201 aatcggggaa tacgcattat attttttctt atacaagagg cggactggaa tcgctcaata
    19261 aaaacggaaa agaatggcta taccgggaaa cacttccagt tttttggcga gcaacgaccg
    19321 ataatgacag aggtagcgga tttggattta aatccggtat gtggttagga gccggattgt
    19381 atccaaaagt caaagaaata gaagtactgg tggacaataa aaagatcgat ttgccgattg
    19441 ctccagtaaa taatcaatat tcaagtaatg aatacgctga aacggttgaa atcattttta
    19501 cactggaaat caacacagta ccaaaaaccc aagtgacaat ttcctaccaa atcgctggag
    19561 agggtaaaat gcttgtcaat atgcattatt atggacaaaa aggattaccg gaattacccg
    19621 cattgggttt aaggttcatt atgccaacga aagcaaaatt ttttgagtat gaaggcctat
    19681 caggggaaac atatccggat cggatggctg gtggtgtcga aggtatttat aaagtggcag
    19741 gactccccgt ttccccatac cttgttcccc aagaatgcgg catgcatatg gaaacgaaat
    19801 gggtagaact aacaagaaat caaacgttgg ataatacgga taaagatcaa gaagattttt
    19861 cgttgcgatt tgaacagcag gatacgccat ttgctttttc cgctttacca ttcaaagcag
    19921 aagaactgga acaggcaaca catatagaag aactaccgcc agaaagacga acggttctta
    19981 cgatttttgc aaaagtaaga ggagtcgggg gtatcaatag ctggggagcg gatgttgagt
    20041 caccttaccg aatcagcagc gaagaggacc atgaaatcgg ctttattatt cagtagcaat
    20101 ctttagatta ttatttgaaa aacggagttt acaagacaaa
//
LOCUS       DAZKOR010000051        19659 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000051 DAZKOR010000000
VERSION     DAZKOR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.051"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..374)
                     /locus_tag="ACG8ZM_001721"
     CDS             complement(<1..374)
                     /locus_tag="ACG8ZM_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492680.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGS"
     gene            complement(374..823)
                     /locus_tag="ACG8ZM_001722"
     CDS             complement(374..823)
                     /locus_tag="ACG8ZM_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492681.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTMVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(827..1444)
                     /locus_tag="ACG8ZM_001723"
     CDS             complement(827..1444)
                     /locus_tag="ACG8ZM_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492682.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(1444..2352)
                     /locus_tag="ACG8ZM_001724"
     CDS             complement(1444..2352)
                     /locus_tag="ACG8ZM_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFQ0492683.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(2365..5124)
                     /locus_tag="ACG8ZM_001725"
     CDS             complement(2365..5124)
                     /locus_tag="ACG8ZM_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0492684.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMDVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(5121..5825)
                     /locus_tag="ACG8ZM_001726"
     CDS             complement(5121..5825)
                     /locus_tag="ACG8ZM_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0492685.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(5837..8146)
                     /locus_tag="ACG8ZM_001727"
     CDS             complement(5837..8146)
                     /locus_tag="ACG8ZM_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0492686.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(8341..8805)
                     /locus_tag="ACG8ZM_001728"
     CDS             complement(8341..8805)
                     /locus_tag="ACG8ZM_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492687.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(8805..9431)
                     /locus_tag="ACG8ZM_001729"
     CDS             complement(8805..9431)
                     /locus_tag="ACG8ZM_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0492688.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(9438..9803)
                     /locus_tag="ACG8ZM_001730"
     CDS             complement(9438..9803)
                     /locus_tag="ACG8ZM_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0492689.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(9793..10131)
                     /locus_tag="ACG8ZM_001731"
     CDS             complement(9793..10131)
                     /locus_tag="ACG8ZM_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492690.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(10121..10447)
                     /locus_tag="ACG8ZM_001732"
     CDS             complement(10121..10447)
                     /locus_tag="ACG8ZM_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0492691.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(10428..10706)
                     /locus_tag="ACG8ZM_001733"
     CDS             complement(10428..10706)
                     /locus_tag="ACG8ZM_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0492692.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(10708..12072)
                     /locus_tag="ACG8ZM_001734"
     CDS             complement(10708..12072)
                     /locus_tag="ACG8ZM_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0492693.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(12085..12654)
                     /locus_tag="ACG8ZM_001735"
     CDS             complement(12085..12654)
                     /locus_tag="ACG8ZM_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFQ0492694.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(12626..13813)
                     /locus_tag="ACG8ZM_001736"
     CDS             complement(12626..13813)
                     /locus_tag="ACG8ZM_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0492695.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEETQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(13877..15604)
                     /locus_tag="ACG8ZM_001737"
     CDS             complement(13877..15604)
                     /locus_tag="ACG8ZM_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0492696.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(15601..16053)
                     /locus_tag="ACG8ZM_001738"
     CDS             complement(15601..16053)
                     /locus_tag="ACG8ZM_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0492697.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(16165..16545)
                     /locus_tag="ACG8ZM_001739"
     CDS             complement(16165..16545)
                     /locus_tag="ACG8ZM_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0492698.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(16542..16928)
                     /locus_tag="ACG8ZM_001740"
     CDS             complement(16542..16928)
                     /locus_tag="ACG8ZM_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0492699.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(16930..17208)
                     /locus_tag="ACG8ZM_001741"
     CDS             complement(16930..17208)
                     /locus_tag="ACG8ZM_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492700.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            17256..17984
                     /locus_tag="ACG8ZM_001742"
     CDS             17256..17984
                     /locus_tag="ACG8ZM_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFQ0492701.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(18130..18606)
                     /locus_tag="ACG8ZM_001743"
     CDS             complement(18130..18606)
                     /locus_tag="ACG8ZM_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0492702.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(18900..19085)
                     /locus_tag="ACG8ZM_001744"
     CDS             complement(18900..19085)
                     /locus_tag="ACG8ZM_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492703.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(19086..19358)
                     /locus_tag="ACG8ZM_001745"
     CDS             complement(19086..19358)
                     /locus_tag="ACG8ZM_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFQ0492704.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(19355..19555)
                     /locus_tag="ACG8ZM_001746"
     CDS             complement(19355..19555)
                     /locus_tag="ACG8ZM_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492705.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
BASE COUNT         5520 a         4200 c         3014 g         6925 t
ORIGIN      
        1 gacccaacca gttgttcaaa actaattggt gtccgatagc cttctggaac agttaaaata
       61 accgaatcat tcccgccact actttttccg tttaaagcca caaaatatat agaaactgtt
      121 tttccttcac gataaagctt tgctgtccct gtattaccgt tagtgacaac cagatttacc
      181 acttcatatc tgttatcatc agtcgtcaaa acacgtttat tattgattgt tacagaacct
      241 gaaaaattct tatgtcctgc aatggcttgg tctccatctg tctgcaccaa tgcttcttta
      301 ccatcaatag cttctacatg cgtttttaag tatttagcaa ctccgtcttc ttttaattga
      361 acaatatcag ccattaaacc gtccccactt tctcaaatgt aatattcgct aatccatcaa
      421 gtttcacttt atcggttgct gacattaaac ctgctgtcgt agttgtagca ttacctggat
      481 ttttctgtgc tccagctgca attccatcca acttagtttt atctgtggac gacatcaacc
      541 catttgctgt agttgtagct acagctgtag ttgtggcatt tattccagga tcacctttat
      601 ctccctttgg taaaacaaaa ttaaatctag ctgcagatga tgttcctaca ttcgtaacag
      661 aagcggttga accactagaa acggttccta tggtaattgt tgctgcttgg ccaggatcgc
      721 ctttatctcc cttcaccatt gttggtttgc cttctatagc attccaatga gtttgtggat
      781 aaacctgtac attgctttgt tttattttta cgatatctgt cattctctat acctccccaa
      841 ttttttcaaa agtaaaattt ggaattcttt cgtttgtgta attttctgct tgatttacag
      901 cttcttggaa tttttgatct acatatgact gattaacacc accagtccca ctaccacctg
      961 tagaactaat tgttccatct tctgcaattg atatatttgc acctgctttt aatattttta
     1021 gagattctaa ttttcctttt aattcttcag agaaattgaa gtctgtttgc ttagttgcag
     1081 ataaaacacc ttcttcagta acttctaaga gttccccaac ttttatacct cctaattgtt
     1141 caggagtagc aatcggcaaa atatatgttc caccttctcc atttacgatc ttttgaaaca
     1201 tttcagcagt aagaatacca tcgcttgtct cgcttgcata aggtagttca gtaattgcat
     1261 tctctaatcc taaatcagct ttcgttaaaa tgactgcacc atatttacca ttaaccgaaa
     1321 gaacttttga ttgtcctgat atcatttttt ctaagcctaa aactgcagag acatgtgtaa
     1381 ttggcataaa ttgacgttta acaccagatt catcagtttc catcattctc tttactttaa
     1441 ccatcaaatc accccaactt tctcaagagt gaaaacattc tgttttggat catcaacagt
     1501 tgcaataatc aaagctcctt cttcaattgg aaattctact gtttcaattt tttcgacttc
     1561 gtgattatct gaaaagagat cgtcttgaag aatatcagtc acttcaattt caccgtattc
     1621 aatcgtgaaa agtgctgctt tcattttttg atacaaataa tttagatcgg ataggaaacg
     1681 ttcagaaatc gattcatggc gtacaccttg aagatctaca cgtgcatcca ttaattcggc
     1741 taacattact ccacctggat cgatagactt caaaatatcc ttgattgatt caaaccatga
     1801 caagtaatct gattcttgtc cttctctcca atcttggaaa ctattctcct gttcctctct
     1861 ccagcgatca aattcttctt tcctttcatt catccagtcc gtaaaatcgc ctttattttc
     1921 attaataaaa tctgtcatat cagcaattaa atcttcaatt gattgccaat aagaacccat
     1981 ttctccttct gtttttgaaa cagcattgat gacaaaataa gagaagtctt gagttgttcc
     2041 aatcaagtct tctcctttaa atataatgaa attggctgtt tgtcgatgca aacactgcat
     2101 ggaatattta tcaaaaatat atttgatttt ccctttttta gcatccacaa tttttgtttc
     2161 taattggact ggatattttc cgccaacaac tgattcaaaa tataccttac attctgataa
     2221 atcatatgga agaccatttt cgacaattgt agcttccata acttcagtgt ttttattgcc
     2281 ttgtcgaact tgaatcatcc ccacgtaatt atagggttct gttgtactta atatgacatt
     2341 ccactttgcc attaaatcac ctccctattt tggtggtatg acaatggaag aaattgcact
     2401 tgcactataa tactgtcgat ctaactttcc acaaatcata cctaactctg tgttttgttc
     2461 ataagtttgc atacgaccat tagctaagcc tcggataacg ccagtatgtc cataagttcc
     2521 atctgcaaac catgaaccca cttgtccacc tcttgcccaa ttaataattg caccaactac
     2581 taattgatca tatctagggt tttgaatcac tttccaacca acagcagacc aatcataggc
     2641 aataccaata tcagatgctg cagaagtatt tcctatcaca tgagttaaac catattttgt
     2701 tccagcaccc atgccacaac cacccagata tcctgaatat tctgcagaca agccataaca
     2761 ctgaccattg ccaattcgtt gtccaatcaa agactctaaa tgttttagtc ctgcttctcc
     2821 agtagctcct cctggtttta aatctttaaa tttgttatac cagttaactg cataatcttg
     2881 acgttcagga tgtgtcgctg ccggacgttc atagtttcgt tcaaaagcat atgctgcttg
     2941 tcttggatca gtacaggcct taaatccatc aaccgtcgta ggttgtacta cgtccatcca
     3001 ttgtccattt gtaaatgtcc aaataagcaa tcgaacttgt gcatctaagc tcgttattgg
     3061 ttctttaata cctgcagcat taaataagtt ttgaacataa acttttccat cccatgttgc
     3121 tggaccaaca agaggatatg aagaaccatc ccactgaact aatccgtatg ctggtccacc
     3181 tatttgaaca gtatcaggat caaaagttcc tcctgtttct tgttgaatgt tccccaatat
     3241 tccacatgca gattgtttcg taaatccgtt attacacaaa atatcgtata ttctccaagc
     3301 tctcttttct gcatctgttt ttaattcatc aggataccca ccaggtgttt ctcctccgcc
     3361 agatggaccg ccattttgtc ctggtatcac ttctttacct ctaacagtaa gttttccctg
     3421 aatatctaaa tcaccgaaat aatatgcttt tccgtttcct aataacacaa atcctttttc
     3481 tgcagttgga gaaattaaaa tgtattttcc atcgccattt gtacgtatta ccaaagaatt
     3541 ttcctcaaga ggtgtaggtg ttacagcatt tggaaatgga tttcctgctg aatctgttgt
     3601 tccgatcgtt ccaatggatt cttttctatt ccagaattcc ataccttttt tggttagctc
     3661 catgattttt ttgttttcat tccaaatttg aagcgtgcct tttacaagtt ttaacacatc
     3721 accataagca ttaaaggaag tttcgaatac ttctgcattt attgttccta ctttgatgaa
     3781 gtcagcaaca atttctcctt ttgatgtgat agcaataccg aacgggccat ttactccgtt
     3841 gtctgaataa cctaagccat ttaaattcca acgccacact cgctttgcat tcgccacatt
     3901 tggagtatcc atgataagaa tctctgatgg agctttttct ggacgaaaaa cgacatgccc
     3961 accagaatta ccagtgatcc atgccgtcgc attcaacaca ttttgaacta acgtttctgt
     4021 tcgattgtcg attttctttt tcaattcttg tgcttgagta tttactgccg aagtgtacag
     4081 tgataaatca ttacctaaaa caatatcctt atatttccca agcgttggga aataagtgta
     4141 ttcgaccata cgctctttta tctcgatatc tagctctttc gccctaacat gagcgacatc
     4201 accaaagtgt aaagacgcta gctcttgata catgtcaccg tattcgagtg tgtgttccaa
     4261 cgccaccata ctgactgtat gagtagcttt tggctcatgg atacgatcat tgtcaaataa
     4321 ggttttgccc catttgatca attcatcaac tgttttacaa tctccgtttt cacgtttggc
     4381 aatacgtcga ttctcgttgg ttactccatc aatttctaaa taaccatatt cgataggttc
     4441 tttgtcttca tcataatcgt tatcaggaac accgccaacc aaatacaaac tattgacaat
     4501 tgattcttca tcaatttctt cttcgattgc ttctaaattg atgccaaaat caatccgaaa
     4561 accattatct gatccaatct gttttaccag tttcaaatca tagttatcca tatcaagttc
     4621 cgcgctagtc acgccagtca aattttgatt accgttattt gaaccaatga ttgcttcaac
     4681 tggcgcgact tgtcttgctg taaattggtg cgttgtccct acattcgaaa gatagttaaa
     4741 tttttgatca aatgccaaac tgtttttaag attggtcatg atctgagacc cattgccgtt
     4801 atccgtaaac gacttgacaa taaagttctt attggccata aaaccaatat gccgagctgt
     4861 tacagaaacg gaggtcaaat tctttttgac attataaatt tcaaaatatt gccatgatcc
     4921 atctggtacc ttcgctttta taaaatttcc tttttttaaa taagagcgat actgtccgct
     4981 tcttgaatag ttaccataaa aacgatattg gccattcagc acacgattga tttctggcaa
     5041 atcttcccaa tcaactaacg acgctccgtt cacgctcaaa tcatctggca tttttttata
     5101 agcataaata aattcttgcg tcacagatac acactcctat tccaaaaccg cacttcttta
     5161 aattttccgg atatttttac ctgattccat tcaggctgca atactggcca atcaccacgc
     5221 gtaaataaat tcaaaccttc ttgtattgct tttccaagct gagtatcaac cacgattgtt
     5281 gcagctaacg tattaagtat tgttaggctt ttatcaccga cagcgatcgt tatatctcct
     5341 ccgttcgatt ctatttccag atagggatgt gcaatttcgt caccatgatc aaaaatatcc
     5401 ataacactag aatgaaaaat tttaggtgct tcaccgattt ttcttttgag tggctgacaa
     5461 cgaaaagtaa catcgaatgt ataaaagaag ccccactcgt tttcgaaagg gacttcttta
     5521 tccatgctgc agatagcctc tagatacttg tctggatcat tatgtgtgat taattggctt
     5581 ttaccagtga gccatcgttt gacttctctc agtttcgaat gtggaatagt gatcccttct
     5641 atttccaaat caaaaggttc atagtcatca aacgtctccg tcaattctcc gcttcggccc
     5701 tgaatcgtgt aagtttcgta tcgcttgttc ggcataatat ctggaagctc tgcctcaatg
     5761 atgcaattca tatcaattac tgcattccga tttttccaaa taaaatttgg ttcatctgga
     5821 ttcataaatt gtcgactcaa ataggaacac ctcccaaatc acgaatagcc tgtttgtttg
     5881 ctttagcaaa tttacgattc atacgatcta actcagatgg attgtttgca tcgactttgc
     5941 caatgtgtat atgctgttca atgctacctc cagaaactct tccactgatc cctttgcgtt
     6001 tttcttcgtc tgataatgga gtaactgtgg ttttaccatt cttggcagtc agtaactcag
     6061 gtccagcttc accaacaatc gcttgtccat tgattagatg accgccttgt gctaaataag
     6121 gcagttttga aatagaaaat gattttccac ctacaccagg aacccatttt ggtatcttta
     6181 tattattcaa tccaccaata aatccattga tcaatccaat catggcatta ataggagctt
     6241 tccctacagc aacaatacca tcgaaaatac ctccaaaaat atcaactaga ccttgccatg
     6301 cttttgacca attccctgta aatactccag tgataaaatc tagaaaacca ctgaaaattc
     6361 gttttccagc attataaaaa ttattgaaat ttgctataac accatcgaaa aatccaccaa
     6421 aataaccact taaaaaatta aatatttcaa cagctacatc tgaaacacct ttgaagaaag
     6481 aattcactcc atcatgaaac catttaacgt tattatatgc ccaaattagt ccagtaacta
     6541 atgcgccaat agctataact gctaatgcaa accagcctcc cgacattcca aataaaccag
     6601 caagtccttg ccaaactcct ataaaacttt taacttcccc aacgattttt gtaacagatc
     6661 ccataagtga accaagaacg attagtactg gtccaactgc tgccgctata ccagcaattg
     6721 ttatgatcca attctttgta tcttgatcta gagaccccca ccatttagag aattcctgta
     6781 ataaggaagt tgctttttca aaaaaaggca aaaggcttac ttggactgct tcacctacgt
     6841 cagccattgc taattttgca ttgttcatag cttgatcggc ttgatcaatt gggtcgagag
     6901 tagcatcgaa tgtgtctcct acagctcccc cactttctcc agcagttttt gctaaatctt
     6961 ccaggttcaa agtacctcta cgaattgcat cggccattct aggaccgcct ttagtcccga
     7021 atacttctgc tgctgcattg atggcttctg tttcagaacc tgcatttttt accttgtctt
     7081 gtagttcttc tagtccttga cttaaagatt ttccgtcttt cgcataagcc actgttgctt
     7141 tagataaact acttaaagct gcgcttgaat caacaccaga ttgctcaaat tgtccaagta
     7201 aagtgacccc ttcaccaaag ctcaacccta attgtttaat ttgtggtgcg ccatcaattg
     7261 ctttttgcat caagtcgtct acagattgac cagtattctg cgatgttttc gttgttacat
     7321 caaggacaga attcaaatca tcatattcta gtccataagc atcaatagct tgtcgagcag
     7381 atattgctga ttgcgaaaca tctgtatcat tgatttcagc gtactgtagg agataattgg
     7441 ttgaatcttc cagtttttta tccatgaatc caaattgtgt atttacctca ccaatagctt
     7501 ccccaaccgt ttgtaaaggt aaatgagtat ttgaaccaac gtttttgaaa gactgtgaaa
     7561 gtctatcagc ttgatcacct gttgctccgg tttttgtaat gatggtatca agtgcctcgt
     7621 caacttcacc aaatgctgca agtcctgctg ctcctgccgc tacaataggc gcggttactc
     7681 caacagacat tttttcacca acgcctttta ctttctcacc tgtttcttcg attttctgga
     7741 gtttttttgc tgtatcaaca gaaacgtcac cttgttcttt aagtgcatcg ttcgtctgtt
     7801 ctaatgctgt acgaagttta ttttctccgg tttcagaatt gagtaattgt ttataaagtt
     7861 tttctgattc agcagaatat tcaccggttt ctttgacaga tttttcatat tcctctctca
     7921 gcatttgaga tcgttgttca gccaatccta attgtttttc taatttttgt ttagccgctc
     7981 gcaatttttc tgtttgagtt gcatccttgt ccatcgcaga cacttggttt ttgtactcgg
     8041 cagcggctaa gttcatttct ttgttgattt ctttgattgt tttcgaataa ctgacttcgc
     8101 cattcgtttt aaaattaaga acgacatcag attctttacc agccatttat cttagcgctc
     8161 ctttctacca ccaaggcgat ttatccatag ttacagattg aggtggttca aaatcggtgt
     8221 tttgttgtaa ccactgtaaa taagatttga gccacaagtt aggtgtagac tttaaaaaga
     8281 aactctcact ccaattcaat agagtgagag caacgtaaat ataaaaactc caaggagttc
     8341 ctatctcttc cgattctttt tgtttttctt tctttttgct tgcgcttttt gaaagtcttg
     8401 tggcttcttg gattttttta ggtcttccac ctgaaatgtc tggctagcaa aaatttccat
     8461 acaggtacta taagcagaca acacctctcc actcattccc aaaaatttga aaatagtttc
     8521 tggatcttcc tctaaaccac cagtacgcag catggcataa atcaaggcac gcatgatttt
     8581 tagatcacta ggcgataagt tagcagaaga aatttgtcct tcttttttag atagcatggc
     8641 gttcatatca gattcaaatt gcgaataatc tccaccatac acatctgcta taaattccat
     8701 cgtttgcata gtaaatgaaa tagggaattc tgcgccttga ataattacaa cagcagaatt
     8761 ttttaagtct tcaacgtgaa ttccataatc agataaccga gccattaacc cgcacctcca
     8821 actttagcca atgttttcca ctgttcttca tcgtatacag gttgagcaat gaatttatta
     8881 aataattcta gagaagctcc gtctctattt gaatcaaaac ttgcatacat cacattattg
     8941 tatttcagtc cgatggaaac cagatttgca gttacatcgt caatttttgt ttcttcctct
     9001 gcagtggcat actcttcgtc aatcacattg gatagttgtg tattaggata ccaaactgct
     9061 tttttaccgc cattttcaat atttccaata aatccaaagg cgaagtacgg aaattctcgt
     9121 gcggtatttt tgctaaacgt tactccgctt ttagcaacta atcctttgat ttcatccatc
     9181 acttctatcg gaatacccac gtgatccaac gcaagttcat gttttgtttc tcgcgacaca
     9241 cgacgaaaca ttttgctgga tgcccatttt tctaatgctg ttccattccc tttgacaccg
     9301 atttttgtgg caattggcaa tcgtaaaact tcactataag tcggtgcagt tccaacagaa
     9361 tctggcgttg ccatcatcgc aattaaaatg tcgtccaatc cttcaaaata aaaagtatct
     9421 tgttttccca attaaaatca cccttcccat aaatcaagta tttttctagt cataatttct
     9481 tcaatttttt ctttgttttg ttcatacgta ccacttgcaa aatgttgggc acgttgattt
     9541 gttgtgccat tttcggcaaa gcgccagtaa aaggccgtct cttcaaattg aactttcact
     9601 tgatcgtttt caactactac ttttatttga tcgcgcatat gtttcttttt taagagcgac
     9661 ttaggaatat taggaagcaa ctgctttaga taaaagttag ctgcttcttc taaagattcc
     9721 aacgataatt ttttcggatc aacttttgaa aggtttccta aataatccga tatttcagaa
     9781 aaaccattct tattacttgg cattttccac acacctcaca tatgtgtaat agttggttac
     9841 ggtatcgtcg ttctcatcac cttgtatacc tgtaaaatct gaatatggaa tgccagcatt
     9901 ttgcagcgct tgttcaataa caactaaatc ctgttctgtt ccaagtgtaa aaaaagagac
     9961 ttggtaataa ggtaattttt tatagacttt accggatgcc atttttttgc tgttactcac
    10021 atttgagtac acaatgtatg gatacatcgt ccctaatctg gctttgtctc tgaacactgc
    10081 taactttgtt gatttcagcg ctgttttcaa ttcatcaaag ctaatcgaca taagctaaac
    10141 tcaactccat ttctcttgca tcgggattcg tataaatgcg agtaatgtta tacgttacag
    10201 aatcaatttt gagcgcactt aatttctctg tgatggattt atcccatctg actttaattc
    10261 gtctgacaac gtctgtcttg gcttgctgtg ataaatattt ttcttgagaa gtcacaccga
    10321 gttcttcata gaatattaga cgcttaaatt cgtaaattgt agttggacga tcgtttccgt
    10381 ctgttcctgt tttgatgtct agcaattcgg ctttccatct gagattatta gtctgtctct
    10441 tcggcatttt gaatcgctcc ttgcacgata aatggcgtca tggcattcat agctttgtcg
    10501 agttcatcct ctgaaactct atattcatag gcaatgccgg caaccatcaa aataagatat
    10561 tcttgttggc caccagttgc tgttttgaca taatcttttg ccatatttaa ataaaaagag
    10621 agcaaagaat catccatgcc ctcttcaaaa tgaatatgtg atttgaattt ttcctctaaa
    10681 gacaattctt tagtttgctc ttccatctta accaccaact ggttttgtaa tttcgtagcg
    10741 atatactgcc ggttcaaatg gagaataaac caattgacca tctagcaagt tgtaaatttg
    10801 gaatccaatt tgatttttac cagagaattt ttcaacaagt ttttgaattt ccaaggcacc
    10861 aataacttct tgaattttaa atgcagaaaa atcgccaaaa tataaaactg gcgtgtctgg
    10921 ttcacccttt ttatctgctg catctgtcca atccacagga tagccaacta attggtaacc
    10981 aattccacct tctgcttgtg tgaatggccg caacaaagga aatccatcat ctgttttcat
    11041 tttttcaatg gcagtcaaag cagctcgatt aataataaag cgtccctttt tcatcacttc
    11101 tgtcactggt gtatttttaa attcgattaa tgcatcatat aatttttgcc cagcacctga
    11161 agcagttaga tctaaaggtt ttttaaatgc tacagccttt ttggctaatg caccaggatt
    11221 ttcatttcca gcgtcatcac cattgaacat ataattgatt tctttacgca cataagcttt
    11281 tttcaattct tccacaacaa tatcttcaac tggaacacca gacattttta ataatttttt
    11341 agttactgtt gccaaagcat cgaattcggc aggattaagc aaaatttcat caaactgaat
    11401 agctgtttca gcaatatcag ttgaacgctc tttcttgttt acattcgcat ctgctttctt
    11461 cacaagaatt ggatatttga catctcctga tgttcgcacc actgttccgt atttacgaag
    11521 taaattttct tcttgagcat aagtaataac ttcagatgca attacttctg ggacagtaac
    11581 tgaaccgttg ccagcttcaa tccctaaagc tcgagcttct gcttcagaaa tatttccagc
    11641 tacaaaatta gcaaatgctt ttcgtagttg ttgatctttt tgtttcttgg tcatttttgc
    11701 acgtgcctcc aatccgttct taattgatcc aagtaatcca tctcgttgct gttgagtaat
    11761 cattccagaa cgattttctg aactatcttc actgtctgaa tcttcttctt gatcgttgtc
    11821 ttcaccagaa cggctttcgt ctgaatccgt actattcgat tgatcatctg tattgtcgtc
    11881 tgtttcatca gttccagaat ctgcgcctaa ttcgtcttta attccgttca attcatcaat
    11941 aacactgtca atttcttctt tcacggcttc taaatctgct tcgcgtaatt ctcctgattc
    12001 aactttttca cgtaattcag tcaatcgttg ctcacgacgt gccttcattt ttttcaataa
    12061 ttctttatcc atgtattttt cctcctacgc ttctagcgct tgattaattt ttttgattaa
    12121 tttttttctt tcttcgacgt ttttttctaa ttcatcacga cttcttaatg cagcttctgt
    12181 atcttcgtat gctggcaaag gcacaataga aacctcgtat aattccactt catggattgt
    12241 tcgaagcatt ggttcagaat tgtagtccca cgtttcttca gtcggcacaa aaccaaaact
    12301 acattggttg atgtctcctc gttccataga tttaactaag tcccttgcta ccgttgtgtc
    12361 cggtaaatca acttcgaatt tcaaaccacg ttcatcttct tcaaggcgaa gggttccgct
    12421 tttagtacgt cctagcacat tggaccaatc gtgattgaac aaacaacgaa catccgaatt
    12481 actaattgcc tttgagaatg caccaggaac aatcacttcg cttaagtcat cccataaaag
    12541 tgtttgactg ttgaatacag cagcgtagcc gctaatggtc ctcgtatgag tttcttcatc
    12601 ggaacgtgtt gaaagattgg tgatgtcaat cgtgcgaatt tccttcttct tcatttccat
    12661 cacctccttt caagtcttga tcattcgttg gtaaggaatc atctgttgca tttttctggc
    12721 caatcctaga taagtcattt gaaatataga tagcttgtgt ttctggagta ttctgtttag
    12781 gaaaaccaag catttctgcc acgttatctg gacttgtaat cccggtacga acgatgttgt
    12841 agccaatatt tgtttttgtt gagtaaggaa caaaatccaa aatattaatt ttccattcca
    12901 ctctatagcc agaattaggc ataaaaaaaa gagcggtgta atgttcgctc ttgtttttca
    12961 atattggttt gattgctttg ttgtgcagat acatcatcgc tttttcaata tctgtcttca
    13021 tcagcgattg atacgtatca acattgattc ctagaaattt tcctaagtct tttttgtaaa
    13081 cacctaaata attcaaaatt gccgcgtcat caacaggact ttttaatgtc tcgatggaat
    13141 atccttttcc caaaggaatc atcttaacag aatgatcact ctcattttgc gtttcttcca
    13201 gttgatccaa tatagctttg acaatttttg tttgggcgct attatttgga ttgatgtggg
    13261 cgtctagttt aagcatgaac gcaagtaaac cgcctttagt atatttgtcc gtcaaaactt
    13321 tttcagcgct cagaacgcct tctagagtgt ttcttgcaag atcaattatt ccagcacctt
    13381 ttaatgaatc agttccgatg ttcttaatgt gtcgaatcat ttgaccaggt attttttgac
    13441 cattcatttc aaattcttct tgaagtcgtt cattgatttt agttgttaca ccgtacgcca
    13501 aatgaagctg atccctatcc gttaatggga atgtctcacc attgatcaat aaagtatttg
    13561 tttccaattt ggtaaattcg aatcctgtca aataatcatt gggactcttc aaaatattta
    13621 gtaagtgatg atctttgact tcgttgccgt cgggaccaat aacaattggt gtggccaatg
    13681 ccacctgatt agatatgtct tgaactaatt cataaacatc agaagattcc atgatagatg
    13741 aatccgtcac atatctttga ccataacgtg tgtagtgtcc aaaaatatcc tcgatatatc
    13801 cacgtttttc cataaaagaa tagactgcat tcgataaccg atcacgtaat ttcaattttt
    13861 ttcaccgcct ttctatttat ctatagatgg aacttaggta atcatctaac tcatctgaat
    13921 caatatctgt catttgattc atcgtttcct tatgaccaca caaaaacgcc acgaacccat
    13981 caatcttttt ctttgattga cgtttacttg gcgctttttg tccattgatg ttagtaattg
    14041 ctacaacgtt caaagtgcaa taaaggaata atggattatc aaattgaatt cgtttctcat
    14101 aaaacaaccg ttcgacatcg tcaaaaggag cattcaatac tttaggatat tgagcaactt
    14161 caacgcattc caatcctaag ttttctaatt tctcaacaag tttgtcactc atcgctggat
    14221 cataattcac ttgctgaata tcatataaat tcatgcagtc ctctatgtac tgcaaaatct
    14281 gatcttgatc aatcattttt ccgtcgcaaa attcaacgaa accttgttca gccaaatcac
    14341 tgtaaggaac attatcttct ttttctcgaa actctaaatc ttcattcgga ataaaataaa
    14401 gttgctttac tttaagaacc gctttaccat cttcatccca tgttgggaag tttaaagata
    14461 cacaggtcaa atctcgtgta cgtgataaat ctaaaccgat ataacatggt tcgccactta
    14521 aatcaccaag ttcttgtgtg gtaactaaac atggctctac ttgatcctgt tcaaagaaat
    14581 tatccgcgcc gttcacaaat acatccaagt gtttcgttag aaattcagct ttggaatgag
    14641 ctgaccgttg cgctgtttta aatgccgatt ctaaagcaga taaatcgact gatattcccc
    14701 agttaggatt gcacatttcc cagacttttc tatccgtcca atcataattt ttatttggct
    14761 catagattag aacaaagttt gaatcattat catcacgttt caacacttct tttgcttcgc
    14821 gatatacacg cattccaaca gacgacgaac ctttcccagc agttgagata ttaaacatca
    14881 atggctgtgg taaagatatc tgagcagact taaaattgtc atactgttcc attttctcct
    14941 gtttatgcaa ttcatcgttc aatacaaaat atggattgga tccctcgata ttatcaatat
    15001 tcttggtttg aacaataaat ttatttgagt aagccatatc ttcatgaata tagtcataag
    15061 taatactaga aacagttccc tttggccccc taaatatttt agttccttca agtaggattg
    15121 gattgtttaa gattgtaact gcaaaaggtt tggcagcata ttgcgcttga gcaaaatcag
    15181 atgcgcaagc atagcaatca actgataacg ccccttctcc gtacattgca tatcctaatg
    15241 aaccgacagc aatcaatgtt ttaccattct tttttggaat ctggacataa gcctcacgag
    15301 taacgcgtac aatttggcct ttttcatttt ctttaaccca accataaatc caagaataaa
    15361 taaatttttc ccagggttct aaaagaaacg gttttcccac catttctcct tttgtgtgaa
    15421 caataaatga ttctacccaa tccatcattt cattcgcgcg atctacatca aaccaaatat
    15481 cttttctttt tttccactga taccagcgat ctattgctaa acgaacggtc ttaggatact
    15541 tcttaggttt ctttcttact tcttttgcaa ataaatcagc ataatttaca ccaggttcaa
    15601 tcatttttca gcacctgcct tcttacgcca tttatttctg tgcgctgcta gttcatctac
    15661 tggctttact tctgggcgtg taatttcttc atcttttctt gcggtcgatc ctccagcaat
    15721 tagtctacca gtttttgctt tgtttgttaa tcctaataaa tctagagctt tcatcttctt
    15781 atctgcccaa gtttctactt gttgtgccaa tggatgtttt gattgattag tagctcctga
    15841 tttattcgtg aatttttgcg tctccggaaa tcctttttcc ttccacaata aatatttgtg
    15901 ttggtaaatt tcaaaaatat ccaagtatga ttcgattaat ggatcaagag tgatggtgta
    15961 caaatcagac aaattcatta tttctaagat acgagctttt tcagcactta ctttttcatc
    16021 aataatcgct ttacgttgcg ctttagtcgt catttttgta tacacccccc tttgtttttt
    16081 gaaaaatttg acctaacgat gcgcgtgact ccccgctacc ctatctcccc acgcgaaaaa
    16141 attttgaaaa tgaatagggg ggcttcaatt gaaatacgaa ggaaaaactt ttttgtcttc
    16201 cgtttcgttt tcaacaattg gatgacattt tgaacataat agcatgagat tgtttggatc
    16261 aagcttaagc agttcgttgt ctttgattgg tacaatgtga tggacgtgtg ctctctttcc
    16321 aaatatgaac tgaccacacc gctgacaatg accaccttct ctttcataaa taaattgacg
    16381 catatctttc catgcttgcg ttcgatagaa tggtttgttc tcatgatgat aaacagactt
    16441 tgcttgctgc ttcttcttgc gtgatctgct tgatctcttg tgttcagtac agtagatacc
    16501 ctttgctatt ttgttcgtgc atccgtcaaa ctgacagtat ttcattctac ttcacgaata
    16561 agatttatga tatcgccttt tgcacggata gcaccaggaa tatcaatacc atgtttctta
    16621 gcatatgcac gcaattcttt tgcagtcata ttgtccagtt catccgtatc ttcagtggac
    16681 tgatcattag tagcttcgtc tccatcaaaa ttagcagcag tgtttccatc gctatcaaga
    16741 atttcactgc tatgagctat gagttcgcca ttgacagcaa caaatgattt gccatcactc
    16801 aaaattcctt tttcttcttt tacttcaaaa tttggttctt gatctttcgg aacaactact
    16861 gtccttttct tttctgaatc ccaatactct gttccactaa tggatgttct aattttgatc
    16921 attgccattt taattctctc ctttgtaatt tgtacggatt acttttgcgc ccattcgctc
    16981 ataccattca atcgtttcct gcagattggg tagagtgtgt gacaataaag aaataactaa
    17041 cgtattcatt ccagttctgt aatcatcatt ctccacttgc acatgtgcat gattaccatt
    17101 ccaattttct ctgtacggac tattctctgc ttcaattgct ttcttataaa ctttagccaa
    17161 acttgattct acttcaatac taagtaccgc ttcatgaaaa tcattcattt gtttactctc
    17221 ctatcagata ttttgtggta tgatttataa aaaagatgcg aggtgtttta atgcttcaag
    17281 aagttaatat taagtatcgt gatcgtcaaa atacagagac aatttatttg ccaaatagtt
    17341 gtccgcattg cggaaatata atgactccgt atgtgtattt tggggtttca tcatatagtg
    17401 cgttcgataa tgatagaatc tttggcgtat tagtacagtg tataaatgat gagtgcaaca
    17461 aattttatag tttacaattt ataaatacgt ttaatgaaga tgaaggatat tctgaaaata
    17521 ttttaattaa agattattac cgtccgccga ttaaaataga tttaccagaa aatattgaaa
    17581 aagtttctcc tacatttgtt gatatttata ctcaagccac acaagctaaa tctgagcaat
    17641 tggaacaaat tgctggagtt ggttatagaa aagctctaga atttctaatt aaagattatg
    17701 caatatctaa aaattcagag gatgaagata aaataaaaaa aatgttatta ggtaaaacaa
    17761 ttgaaaatta tttgaatgat tttcctaaac tccaaagatt agccaaagct gctacttgga
    17821 ttggtaatga tgaaacgcac tatgttcgta gacatactaa taaagatgtt catgatatgg
    17881 atgcatttat taaatcagca gcgcaattta tcgcggctga ttatgatgct gatttagctg
    17941 atcaatttat aaatggaaat aactttatgc caactactaa ttaaaagaat aaaagagcca
    18001 cagataccaa ttaataaaac ggttgggata tcttggctct ttcatatttt tttgacacta
    18061 atagaatatc atgctgctaa caatgtttac atagctacaa tgaccgaaca tttagtgaac
    18121 agtgtaattt tagaataaag agagttgttc tttgtacttc tcgctattgt aaaaatcaat
    18181 ttcgttttct tttttcaatc ggtcagcttg tctgtgttca tactcatcta aaaagttcaa
    18241 tgtctttcta atttctgcat gtcgctgtct gacatacgat gagctatacc caacagattc
    18301 agcaatatct tctaagctca tttgatcaac atatttcatc ttaacaattt gattatcgat
    18361 accagaaaag ctatctatca gcaataacat ttcttctttt tgctcgatca acaattccaa
    18421 ttcattttct atcttttgga tattctcttc taaagaagaa gttctcgaat tcttttcgat
    18481 gcggacgttt gctaaatcac cacagaccca tcgattcaat tcaagcttac ttttattaag
    18541 attccacttt aagtaaagaa tctgctcatc tagttcttgg taatctttta accaatgaaa
    18601 tctcacaaac gccacccctt atggtagaat agtattgcgc tgctatctcg aaaggggtag
    18661 cttttttatt ttttcttagc cgttccattc tggattgcct tttgttccaa acgtcgtttt
    18721 ttcttcttaa tctttgattt tgtttttccc atacaattct cctttccgaa aacacatttt
    18781 gttcgatttt taatactaaa taaaagttgg ccagaatttt tcgttcgtct ttactcatct
    18841 ttttttgaat aagattacta gcataaaaat agttagtacc acgaaaatga acaggtaaat
    18901 caattacgtt caccttcttc atgccagtta tcagccacta gaatctttgc gttagtgccg
    18961 ccaccgtttt cttttttgta tttttcaaat gctttataag cagctgtttt tgatttatag
    19021 atttttccgg aaccgactgg gaacatccat gtgccctctg tgatagctaa gatgtatact
    19081 ttcatttaat tcaccccttt aatccccaac actacatatc cggacttctg catgtaatca
    19141 gtaatataag tgatctctgc tttgtatgag tcaccagtgt aactaaatgt tggtctcgtt
    19201 agtccaggta attcatgttt aatgtatttt ttatattcac gtagtattaa ctgatcacca
    19261 actttgaagt cacgatcatt cttgcggatc tcgaattgtt tgcgtccgct cgtcactgct
    19321 tcaaaatatt ctggtagtat ttttagttcg tggatcattc tgtttcctcc ttcaaaattc
    19381 gctcatcatc aaaaagtgaa tctccacaaa atccttctcc caaatattta tcgttcatgt
    19441 ataagtcaac caaatatttg tgatctgctt ctcgatattc attaatatta acgatcttta
    19501 ttttgtaaac attaccttcg tcgctcacta tcttgaattg ttctctgact ttcaccaccg
    19561 ttcctcctct ttttgaaaaa attttagtgt ttttgtaaga ccccctaaat cagctttttt
    19621 atttgatata atacttgtat cgattaatca aatcgaaat
//
LOCUS       DAZKOR010000052        19507 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000052 DAZKOR010000000
VERSION     DAZKOR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.052"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG8ZM_001747"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG8ZM_001747"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0492706.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACG8ZM_001748"
     CDS             complement(1173..1868)
                     /locus_tag="ACG8ZM_001748"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0492707.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACG8ZM_001749"
     CDS             complement(1881..2597)
                     /locus_tag="ACG8ZM_001749"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0492708.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACG8ZM_001750"
     CDS             2927..3532
                     /locus_tag="ACG8ZM_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0492709.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="ACG8ZM_001751"
     CDS             3857..4180
                     /locus_tag="ACG8ZM_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0492710.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="ACG8ZM_001752"
     CDS             4362..5732
                     /locus_tag="ACG8ZM_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0492711.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="ACG8ZM_001753"
     CDS             complement(5862..7163)
                     /locus_tag="ACG8ZM_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0492712.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="ACG8ZM_001754"
     CDS             complement(7393..9384)
                     /locus_tag="ACG8ZM_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0492713.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="ACG8ZM_001755"
     CDS             complement(9528..9794)
                     /locus_tag="ACG8ZM_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0492714.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG8ZM_001756"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="ACG8ZM_001756"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0492715.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG8ZM_001757"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="ACG8ZM_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0492716.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /gene="def"
                     /locus_tag="ACG8ZM_001758"
     CDS             13138..13701
                     /gene="def"
                     /locus_tag="ACG8ZM_001758"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0492717.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="ACG8ZM_001759"
     CDS             complement(13954..14475)
                     /locus_tag="ACG8ZM_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0492718.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="ACG8ZM_001760"
     CDS             complement(14478..15320)
                     /locus_tag="ACG8ZM_001760"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0492719.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="ACG8ZM_001761"
     CDS             complement(15313..16038)
                     /locus_tag="ACG8ZM_001761"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0492720.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="ACG8ZM_001762"
     CDS             16214..16510
                     /locus_tag="ACG8ZM_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492721.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16766..16940
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17078..18670
                     /locus_tag="ACG8ZM_001763"
     CDS             17078..18670
                     /locus_tag="ACG8ZM_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0492722.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="ACG8ZM_001764"
     CDS             18683..19423
                     /locus_tag="ACG8ZM_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492723.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4002 c         3702 g         6223 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaa
//
LOCUS       DAZKOR010000053        19150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000053 DAZKOR010000000
VERSION     DAZKOR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.053"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..636
                     /locus_tag="ACG8ZM_001765"
     CDS             133..636
                     /locus_tag="ACG8ZM_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0492724.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="ACG8ZM_001766"
     CDS             763..1800
                     /locus_tag="ACG8ZM_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic GMP-AMP synthase DncV-like
                     nucleotidyltransferase"
                     /protein_id="HFQ0492725.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="ACG8ZM_001767"
     CDS             1815..3512
                     /locus_tag="ACG8ZM_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E2/UBC family protein"
                     /protein_id="HFQ0492726.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="ACG8ZM_001768"
     CDS             3505..3966
                     /locus_tag="ACG8ZM_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFQ0492727.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..4839
                     /locus_tag="ACG8ZM_001769"
     CDS             3979..4839
                     /locus_tag="ACG8ZM_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492728.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4877..5266
                     /locus_tag="ACG8ZM_001770"
     CDS             4877..5266
                     /locus_tag="ACG8ZM_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0492729.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5253..7700
                     /locus_tag="ACG8ZM_001771"
     CDS             5253..7700
                     /locus_tag="ACG8ZM_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0492730.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            7705..9813
                     /locus_tag="ACG8ZM_001772"
     CDS             7705..9813
                     /locus_tag="ACG8ZM_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0492731.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9810..10814
                     /locus_tag="ACG8ZM_001773"
     CDS             9810..10814
                     /locus_tag="ACG8ZM_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0492732.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10833..11744
                     /locus_tag="ACG8ZM_001774"
     CDS             10833..11744
                     /locus_tag="ACG8ZM_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0492733.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            11931..12218
                     /locus_tag="ACG8ZM_001775"
                     /pseudo
     CDS             11931..12218
                     /locus_tag="ACG8ZM_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12406..12621)
                     /locus_tag="ACG8ZM_001776"
     CDS             complement(12406..12621)
                     /locus_tag="ACG8ZM_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492734.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12880..13089)
                     /locus_tag="ACG8ZM_001777"
                     /pseudo
     CDS             complement(12880..13089)
                     /locus_tag="ACG8ZM_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(13392..14831)
                     /locus_tag="ACG8ZM_001778"
                     /pseudo
     CDS             complement(13392..14831)
                     /locus_tag="ACG8ZM_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15379..16050)
                     /locus_tag="ACG8ZM_001779"
     CDS             complement(15379..16050)
                     /locus_tag="ACG8ZM_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0492735.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16399..17850)
                     /locus_tag="ACG8ZM_001780"
     CDS             complement(16399..17850)
                     /locus_tag="ACG8ZM_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0492736.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(17962..>19150)
                     /locus_tag="ACG8ZM_001781"
     CDS             complement(17962..>19150)
                     /locus_tag="ACG8ZM_001781"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0492737.1"
                     /translation="VSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKD
                     GTRFSQEYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGI
                     IGAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSE
                     SPKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQ
                     QTNIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTN
                     GFSIFNYNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKR
                     IVGVQLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLN
                     IET"
BASE COUNT         6238 a         3214 c         3616 g         6082 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     4201 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     4261 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     4321 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     4381 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     4441 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     4501 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     4561 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     4621 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     4681 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     4741 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     4801 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     4861 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     4921 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     4981 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     5041 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     5101 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     5161 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     5221 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     5281 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     5341 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     5401 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     5461 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     5521 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     5581 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     5641 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     5701 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     5761 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     5821 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     5881 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     5941 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     6001 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     6061 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     6121 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     6181 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     6241 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     6301 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     6361 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     6421 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     6481 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     6541 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     6601 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     6661 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     6721 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     6781 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     6841 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     6901 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     6961 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     7021 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     7081 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     7141 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     7201 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     7261 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     7321 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     7381 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     7441 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     7501 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     7561 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     7621 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     7681 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     7741 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     7801 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     7861 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     7921 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     7981 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     8041 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     8101 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     8161 ctaggagttt atgtggcgta tacaggatta ttaaaacgag aaactaccaa agcagtccgg
     8221 gctgtcatta attttgtgat gatttttcta ctttcgggtt cgttcattgc gtatgcgccc
     8281 acctatatca caaaaatcaa tgattttagc tcagatgtca gcgaagcggc tcttagcctt
     8341 ggaaccgaga ttgtcgtgcc gaattcagaa agccaaggaa aggacagtgt ggatttaatc
     8401 cgagatagct tgttttccat tcaagtccaa caaccgtggt tactcttgca atttgatgat
     8461 tccaatatag aagaagttgg cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat
     8521 gaaaataaag gcaaggatcg ggaagaagcg gtcaaagcgg agattgaaga taatgacaat
     8581 gcgaatctca gtattaccaa gaccatgaac cgcttaggga ccgtggtctt tttggtgttg
     8641 ttcaatattg gcatatcctt ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa
     8701 attctcttta ttatctttgc gatgtttttg ccgattagtt tcttattgtc catgttgccg
     8761 acttatgaga gtttgggcaa gaaggcgatt attcgcttgt tcaatacgat tatgatgcga
     8821 gcaggagtta ctttggtgat tacgactgca tttagcatct ccacgatgtt tttcaatatt
     8881 tcggcaacct atccattttt catggttgct tttctgcaaa ttgtgacctt tgccggtatc
     8941 tatttcaaat tgggcgacat tatgaatatg ttcaatctcc agagtaatga tagccaatca
     9001 atgggaagac gggtcatgcg aaaaccgcaa atgttaatga atcgaacatt gcggcaactc
     9061 aatcgaaatg tgggacggac tttggctttt ggtggtggtg cagcagttgg aaataagttg
     9121 gcaaaggaac aatcaaaatc caaatttaag ccatccggtt cttctctacg aaagaattca
     9181 cgattaccga acgatcacga gataccatct gacttgaata aggaaaagcc aattccaaat
     9241 aataagaaac aatcccgaat gaatttggcc gggagaaaag tcggcaaggt tctggatacc
     9301 caagctttgg tgaaagataa gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc
     9361 aacttgaaat acaacttcca taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt
     9421 aagcgaggac tggtccaaga aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa
     9481 cgtagagacg aaaaaatagc tgaaaagcgc aaagtattga acgaaatagg gaatcctcat
     9541 gggaaaagaa gggaaaatgt tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca
     9601 ccaaagcgga ttgtgagagc aaattcgaat cccgaaatca aacgaaaatt cgctagccaa
     9661 gaaatcattt caaaaggaat aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg
     9721 aaacggcggt ctactctgtc caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct
     9781 catcccaaac caaggtcagg tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt
     9841 taactttcct aacaatctct tttatgggta tcttgatgtg tgtgggctta ttgtttggtg
     9901 aagacagtga aggaagtagt agttcaggtg ctcctcctgg ggggagtagt gtctcagaag
     9961 aggttttgaa acaccgtgca atggtggaaa aatatgccaa agaatcggat ataacagagt
    10021 atgttccgat tcttttagcg attattcaag tggaatctgg tggcacaatg gaagatgtga
    10081 tgcaaagttc tgagtcagcc ggtttaccac cgaattcatt aagtaccgag gcatccatca
    10141 aacaaggatg tctctatttt gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc
    10201 aagatagtat cattcaggcc tataactttg gcggtggtta tttggactat gtggcaaaaa
    10261 acgggaagaa gcatagtttt gctttagctg aatctttttc aaaggaaaaa tcttgcggtc
    10321 aaaaaatcga ttaccctaat cccatcgcga ataaggaaaa tggtggatgg cgctataact
    10381 atggcaatat gttttattgc ttattggtga agcaatacct aactacaaca cagtttgacg
    10441 ataaaacagt ccaagggatt tttgacgagg cgtttaagta tgaaggaacg gcttatgtat
    10501 ttggtgggtc aagacccgaa acaggatttg attgtagtgg cttgactcaa tggtgttatg
    10561 ccaaagttgg tctgaaactt cctcgggtgg ctcaagatca gtatgatgcc atgacccata
    10621 ttgacctaaa agatgccaaa ccaggggatt tggttttctt tcattcgact tatgatgccg
    10681 gtacttatgt gacccatgtc ggtatttatg caggggagaa tcgcatgttt catgcaggag
    10741 atccagtagg ctggacgaat ctaacagaaa gctattggca gcagcatctt atcggtgctg
    10801 gacggtacaa ataagagaaa agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa
    10861 aggaaaagag cctaaaaaag aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa
    10921 tggtgcttgc cctttggctt ttattatctt gtagtcttac ttttggcgtt tataaaaatt
    10981 tcaccgccat tgatcaacat acaactcatg aaaaagtggt tattaaagag aaagtagtga
    11041 atacttctgg tattgaaaac tttacgaagg atttttcgaa agaatatttt tcgtggaaaa
    11101 ataacaaaga agcaattgaa aagagaatga acaacctcga acagtacctt acagaagaag
    11161 gtttcgcttt aagtcaagat atggttcgag cagatattcc cactaattcc gaagttcagt
    11221 cagtaaaagt cttagatgtt gaaaagagct cggaggaatt tgtcgtttca tttttagtgg
    11281 agcaaaagat tacagaggga aaaaaggcac aatcgatttc ttctgcatac cgagtgacga
    11341 tctttgaaga tgaaaacagg aattatatag taacgagttt gccaactatg attgcaaaac
    11401 ctgatagggc aaaatacaag acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa
    11461 ctacagaaga aatcgctgag tttttggaaa cgttctttaa attatatcca tcagcttctg
    11521 aaaaagaatt ggagtattat gttgaaaatg atgcgatgcg accaatcaat attgacttaa
    11581 agttttcgaa gttagaggat ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta
    11641 caaagataaa ataccttgat aatgtatcaa aaaccactag ctactttcag tacaaattaa
    11701 tattacaaaa aaatggaaac tggaaaatca ttgatgttaa ataataatga ctcaatctaa
    11761 atttatgaga agttataagt agtttaactt atttcaggca gaagtcgccc atcctctata
    11821 ggtgggtgat gaatgccgtt cggtaactcg aaaaccgaca attaagacat agtgcctttt
    11881 tattttaatc catgtcataa tcagtctata taagaaatgg ttgatatacc atggaattag
    11941 atacaaataa tcattcatta tttcttcttt gttaccacct tattttagta gtgaaacatt
    12001 gtaaacatct cattgatgat gtaatgttag cttatgcaaa aaactatctt tgaaagaata
    12061 tcagaatcac atcacattac cttagttgag tgaaatcgtg ataaagacta ctttcatatt
    12121 atgctcaaag cacaacctaa aaccgaatta acaaaattca gcaatgccta agctcatgtc
    12181 tgataaaatg agactttcta agggtcaaaa atttcctttg aatacgatca ttagcttaga
    12241 taaaagtaaa aaccttagaa ctacggggat agcttggtta ataagagata tcgctgttaa
    12301 taaagacata agcttgcaag tatgctctat tcccaagaat ctcccacttc aagcactaag
    12361 gatcgttaaa ttcagctaag tggcaagtag ttcactagac gataattatc tccatatttc
    12421 aatctcagct tttccatttt tcatagcaaa ttccgttatt tttatagcat ctaaaagatt
    12481 cagcttttcc actgatcttt caccactttt cagctttttt attgtgctta aattgatacc
    12541 agtacctttt gatattgaat tggaagtaaa atcattaagg gtttttttta ttaaattcat
    12601 atccgcaatt ccagcgttca taagaatgaa gattctcctc tcatagacta ctttgaaaat
    12661 ggtactacag tggctgttta tatatttcag taggttactg tagtggtcat tttattataa
    12721 ttagaaaagg aacctaaatc aaaaatgcta aagaaaattt tcatagtatt aaacctatca
    12781 tgactaaagc cacggaattc ttgataaaag agtatacttg caagcctatg tctttactaa
    12841 cagcgatatc tcttattaac caatctatcc ccgttgttcc taaggttctt gatgttttct
    12901 aaggcaatgc ttgtatacat agcccttgat caaaatattg atactaacgt tgacgtttcg
    12961 atcatgatgt gtacggcaaa taggacaagt ccattctcga attgtgaatg acttcttacc
    13021 atctgtgtgt ccacattctg aaccaatttg actagacgga aatcatttat ctatcttgat
    13081 gatttttttg acagaagtat cgaaaatctt tacataatct gggtgttgca ttcttatgca
    13141 gatgagagcg ttagctccgt attcataacc agttgcctct aaaacgtagt ccatcggtgg
    13201 atacctagct tgcggttgcc agtggctttc gcagtcttct tgtaatcaaa tcgagcatct
    13261 aattgagtac tagtattttg aagataatac ttgagttttt cggttttcag aagttcactt
    13321 catcttccat catccatgga aatcccactc acccatacgc aggcctccaa aaattcttca
    13381 acaatggttt ttcagacatt tttctgaata cctccttgtt ttttgatacg ccagatagtc
    13441 aggaaaaagg caacaaaaca caaaacaaag ctgcctaaaa aggtaatctt cattccatat
    13501 aaaaagacat ccggccgatc tgcgatatag ttagttactc gttggcccat cttagcagac
    13561 atcgcttgat ataagaccgt cgtagaaagc gaaatcccga tgaccatccc caaatttcgg
    13621 gcaaaggaat tcatggagcc tgccagtccc aaattttcga tagtgacaga actcataacc
    13681 atcgtattat ttggggactg gaacaaggca ttgccggcac ccatgatggc agtagcgaca
    13741 atgtacagcc acaaaggaga gtccactgac aaaaacatat acatgacttg ggttaccgac
    13801 aataaaccca agcctactaa tacaaaaatc tccgggccaa cccggtcggt caggtatccg
    13861 cttaaaggcg ccccgacgac catcatcaag ggaaagacca tcatcaacag tccggcatag
    13921 ctaggactca acccgcgagc attttgcaga taaaaaggaa tcactacatt aacaaagaag
    13981 ttggaagaaa aaatcaacaa ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa
    14041 ataccgaaag aaatcaatgg agaggctgct ttcttttcaa cttttaaaaa gctgacaaag
    14101 gcaatcactg aaattacgaa cagtacaagg gggactggat ggaaaaagcc actttcttgc
    14161 cccaaaaaga ttgttccaaa aaggctgata atcgtagccg caatggcagc aaagccccct
    14221 ccatcgattg tatggccgct catggtgata tcttttggta agaatttttc agcaatcaac
    14281 atcgttacga ttcccaccgg tacattgatc caaaaaatgt acgcccaaga aaattgggac
    14341 aaaatcaagc cgccaagacc tggtccagca atcgagccta atgaaacaag cgcaccgatt
    14401 gctcccaatg cccgaccacg ttcatgtatg ggaaagactt ccgtgatgat tccggcattt
    14461 gtagccatag tcatactagc accaatggct tgtaccaccc gtgcgaacaa ttaaatacct
    14521 agcgaatgat tgaacccaca caacagcgag cccaagacaa agatcagcgt accaatccga
    14581 aatactttga ttttcccaca gctatcgcca attttaccaa acagcaacaa acaagaacag
    14641 acaatcatca gataaacgga cactacccat tccgcttgat tcattggtat gcccatatca
    14701 tctgagatgg taggtagtgc aatattgaca atactgccat ccaatgtgga catgaaggta
    14761 aacatagata tagcaatcaa aatccaccag cgattttttt ggacttttgc atcttcttga
    14821 taactatgca taataggatt tcctttctaa tgatcgtgta gctaagaaca atataaccta
    14881 tgtctactgc ccaacaactt ctcgcgactc tagccatagg aagcttgagt aatttcgatt
    14941 tgttattctc atgtaaccat gtaagtttga gatgctttat ttgaacttac tctatagtaa
    15001 ctagtgataa ttcgctttac tttcttctag caatttctaa aagtcgactt cattgttctt
    15061 cagattaata gtgtgagcta tctcgtgaga cgtaagaaaa tttccattct actttttccc
    15121 aattttttca tatttatggt gttgcaccta tttgttttcc aatttgcttt tcaaatacat
    15181 tgctatgata ttcaattcca taggattata ctaaatgtca atagtatgaa taatatatta
    15241 tagatgttta tatatcactg ttaattatta tccacaataa ttaatgatta cctcgatatt
    15301 tgtcaatcaa aggttgaaac aatttatttt cagcatcttt tctaacttga gctgcactct
    15361 taatagaatc atacgatcct aaatagtacc tctttttttt aaagttatat acgcaatcca
    15421 tttctcttta gaacgataaa ctcccttaac accacttgta ttatttttcg gtaattttga
    15481 cgttagacac tctattactg tcccttcaac acatgtcctt gtttcttgcc tttttaaaca
    15541 tccacaactt tgagtatgtc caaaaaataa gttactataa cttatctcac acatactgcc
    15601 acactcacat tgacaaaccc attttgtttt ccatgaacag ttattgtcca aaggatactt
    15661 tgcagtaagt aatccgaatt ttttattttc aatgtgatgc ttttttgatt tcaaacatcc
    15721 acagcttttt acatcacctt ttagaagtcg ctttgccgat actaactttt ttctcccaca
    15781 cagacattga cattcccaaa caacagttcc ttcacttcgc agtttagttg gcttgattgc
    15841 aactaactta ccaaaagttt tatttgtcaa atttttatat ttatgcctat ttttataatt
    15901 tccacatcca caacttttgg ttctacctga tagtaagttt gatcgatata caatcgtttc
    15961 attcccacag tcacataggt aacgatattt tttatatcca ttatgagtaa tctcctcaac
    16021 aacagtaagg tgaccatatc tttccttcac tgcacctacc ctcccaagaa agaagtacca
    16081 tataatttca attaactttt aataaatcag aaaaaaatat tttcaatttt tcttagtctg
    16141 attttatgac taatttataa tacaagactg aacgatacgt acacgaaatc ctcaagatag
    16201 ataaattgtt ttcatctagt aaaacctgct caaaatatga gcataaagac ggcaataata
    16261 cactcgatat tcgataatgg acttgtttta tttgtcaaac tcatcgtaat cgagatatca
    16321 attccagtct caatatcctg atcaaaggtc taagaatcca accattttct tgttactttg
    16381 tcagattgat tacctttttt agtctccgaa taataaggtt ttcggatcaa ctaacatgct
    16441 ctgagaaaga ctactatctt tgcaaatttc caaatgaagg tgtgggccag tactattccc
    16501 agtacttcct acatagccaa ttatttgtcc ttgcaaaaca ttatctccag tttttactag
    16561 gtattgttgc tggtgagcat ataacgcggt tgatccatct tcgtgttgta tcaccacgta
    16621 atttccccaa gagtagtgaa actctgcttt aacaacctta ccagctttaa ctgcatgaat
    16681 cggctcaccc tgtgtagctg ccatatcaag tcctcggtga aattcatttc cacgatttcc
    16741 aaacaaactg gttattgaat aattatgaat aggcacttgg tatccagtct tactcatttg
    16801 tggaccttta acttctttat catagttggt taaatcatag gttttaatta attcatttag
    16861 cttcaaatag tattgtgtat ctgttgcata ccgtcctgta aggaatttag ttgcttctcg
    16921 atatgtttta ctattagctt ttaatgctcc tgcataaaaa tctttatttc cactaattcc
    16981 cttagttaat aatttggcat aatcttcaaa actttcctca tagttttcat acactctaaa
    17041 ttttgattgg gtcgtataaa gcgttccatt acccaaatct tcgtttgtta ccataatgac
    17101 aaagtttcca ttataagttc cttttatccc aaataaattg taattcggtg cctgagctaa
    17161 ctggctttga ccactggcag attctaagat cgcttgtgca atcataacag aagcatataa
    17221 atcatatttc tgaccaattt ttctcgcaga ctcaccaatt ttcataataa aattttctac
    17281 agattcattt ttgtcaaaat gaatttcttc attaccagat agtattggtt gtgattggaa
    17341 agattctgtt gattctattt gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt
    17401 tggcgcttcc ttttttgaag actctatatg gtttggtctt gtcactttcg aagaagttat
    17461 ttcacttgta ctacttgaag ttgttgaaga ttcggcactt gaaatcgtac tactttcact
    17521 tgacgaagtt gttgaagact cggcatttga agtcgtacta ctttcacttg acgaagttgt
    17581 tgaagactcg gcatttgaag tcgtactact ttcacttgat gaagttgttg aagactcagc
    17641 atttgaagtc gtactacttt cacttgataa agttgtcaaa agttcactac tctctgctgt
    17701 tacatcggaa ttactcattt cagtcgtctc ggttctttct tttgcaattt tgtatgttga
    17761 attttcgctt gccaacacat ggaaaggtgt tgctataaag agttgggaca ttaatataac
    17821 tgctgatgca agttttaaag attttttcat ttgatttacc tctctttttt ctctttaata
    17881 gtgttttcta ttaaaaatta tgataaatat tttcaatgaa ttgagtaata cagtatattt
    17941 cttgatttca tttagtaacg attatgtttc aatgtttaat cctgttactg cctttaaaat
    18001 aaaattatag ggttggctaa aactaggatg aaataaataa tctaacattt tcaatttgtc
    18061 taatgtaatt tgttcttcta tagcaagaga aaacattgat aatagtccag ataaatcttc
    18121 ttgactacaa agttgtacac ctactattct ctttgtattt ttttcaaagc taattcttaa
    18181 ttttacacga ctattttttc tcaaaaaaac aggtctaatg aaatcttcta tttctatata
    18241 atctatagac tcacttaata atcgtgctgt ttttaaattc aaacctgctg cgtaaaaatt
    18301 gtaattaaat atagaaaagc catttgtaaa ttgggaacca gaaggtgtaa aatcttgatt
    18361 gtcaatatgt tgagttgcaa tataagctgt tcttagagca ttagaaatag aaaaatcaac
    18421 actacttcta ttaatggacg taaaaaaagt tgtagcacaa tccccaatag catatatatc
    18481 ttttatattt gtttgctggt attcattagt tagatatgct ccactaacat ctctcaagag
    18541 ttcctttttg ccaattggac tattagatag aaatcctata gcttgtacga ccatatccat
    18601 agagtattgg gtaccacttg tagtgactat tttttcaatc ttttttcttc ctaaatattt
    18661 tttaggactt tctgatagac aaagatgtat tccatttttt gatagtattt cttcaacttt
    18721 atctgaaaaa ctacggtcgt aataagtcga caacactcta tctgcaacat cgaaaagata
    18781 tacttctttc cctctttttc ttatggcttc agcaatttca actccaatat atcctgctcc
    18841 aataattcct acttttttaa tagtattatc atctaaagct ttaaaaatct ctttagcatt
    18901 tggaaaattc tttatcgaaa aaatattagc taaattattc cccggtacat ctaacataat
    18961 ttgctttgaa cctgtagcta gtataagttt gtcatattct tgggaaaatc ttgtgccatc
    19021 tttttttaca caaataatct ttttattttg aatatcaatt ttgtcaactg ctgtctccaa
    19081 aaacaacttt tttatcttag aatttagttc tcttctattt tgataaaaaa gatcttctaa
    19141 attgctaact
//
LOCUS       DAZKOR010000054        18619 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000054 DAZKOR010000000
VERSION     DAZKOR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.054"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(4..915)
                     /locus_tag="ACG8ZM_001782"
     CDS             complement(4..915)
                     /locus_tag="ACG8ZM_001782"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0492738.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1292..1930)
                     /locus_tag="ACG8ZM_001783"
     CDS             complement(1292..1930)
                     /locus_tag="ACG8ZM_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492739.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1934..3916)
                     /locus_tag="ACG8ZM_001784"
                     /pseudo
     CDS             complement(1934..3916)
                     /locus_tag="ACG8ZM_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4096..4761
                     /locus_tag="ACG8ZM_001785"
     CDS             4096..4761
                     /locus_tag="ACG8ZM_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0492740.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5158..7191)
                     /gene="brxL"
                     /locus_tag="ACG8ZM_001786"
     CDS             complement(5158..7191)
                     /gene="brxL"
                     /locus_tag="ACG8ZM_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0492741.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7309..9831)
                     /gene="pglZ"
                     /locus_tag="ACG8ZM_001787"
     CDS             complement(7309..9831)
                     /gene="pglZ"
                     /locus_tag="ACG8ZM_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0492742.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(9947..13579)
                     /gene="pglX"
                     /locus_tag="ACG8ZM_001788"
     CDS             complement(9947..13579)
                     /gene="pglX"
                     /locus_tag="ACG8ZM_001788"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0492743.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13643..17314)
                     /gene="brxC"
                     /locus_tag="ACG8ZM_001789"
     CDS             complement(13643..17314)
                     /gene="brxC"
                     /locus_tag="ACG8ZM_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0492744.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17339..17914)
                     /locus_tag="ACG8ZM_001790"
     CDS             complement(17339..17914)
                     /locus_tag="ACG8ZM_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0492745.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(17914..18513)
                     /locus_tag="ACG8ZM_001791"
     CDS             complement(17914..18513)
                     /locus_tag="ACG8ZM_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0492746.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5736 a         3560 c         2610 g         6713 t
ORIGIN      
        1 cgcttattta ctacttgctg tactagaatc tgaagtgaat gtactcaaaa cattttcaaa
       61 gtctttgtct ttgattttta cattagcgtc tttcaatact tcgccaatta ctttattctg
      121 gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt
      181 atccatgtcg ttgcctttgt tttggttttt caccatttta accacataat attccgtagt
      241 ataagttgaa gcattggtag aagtgatcac atcagaaact tgaccatctt tcaacttaaa
      301 ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc
      361 gccatcttct tttgtcgtag tatcagttga cttgtctttg gctaatttag agaaatcgtc
      421 tcccttatca gcagctttct tcacatcttt tgcatcatct tcacttgcta cttggataat
      481 ttgagcttcc acttctggat ggaaagattc ccaagccgtt tttaaatctt catcggtcaa
      541 tttgatatgt ttctttagac cttcttgaaa ggctagactt tgtttgatac tgtctttgaa
      601 tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca aatgaatcac ccaattgttt
      661 ctttgtttga tcatattggg aatcgatttc tttggttgat acgtcatctc catatttatc
      721 ttcaaatact tgataaacga tcaactgttg cacagcggaa tctgctcctt gcattttttt
      781 caagctatcc attacttctt gttcgctgat tgtaccgcct ttataactaa cggctgtttt
      841 ttcaccggca ttcgtacagc ctgacaataa gccagtgcct agtacgatgg caagtgctaa
      901 aaatcttttt ttcatttatc aattctccta cttttcgttt tttgcgaatt gctaaaaaca
      961 aaggtgttca agatgaaaat catcaagaac acctttacta atctgctcga tacgtcctga
     1021 atagatataa ggctacacaa tccagaaagt tcgctcagca gaataaatgt caagtagcac
     1081 atcacacctt aatatactac ttcttttgtt accgtttttc aagtctatct tgcgttccgt
     1141 atttaacgta actaattctt agaacaaatg ttatcgattt tgtttttctt cttccgctgt
     1201 tgggatattc aaatcagaca cttttatgtt cgattttcca gacatttttt tgttgttttt
     1261 attagaaaaa gtttgttttt tcatttgttt tctaaagcat gatcttcttg tttcctattt
     1321 tggtcagata tggatcatgc gtgaccacga taactaacgt accttgggaa gcctgttgcg
     1381 ataagatttc aacgacttgc tccttatttc gtttatctaa tgatccagtt ggttcatcgg
     1441 ctaaaatgat tttgctattc ttcaataaga ctctcgcaca ggcgattctt tgcttctcac
     1501 caccagataa tgtgtagata ggcgtactta gcttcttgtt tatatttact tttttcaatg
     1561 cctcatactt tgctgttcgc ttatctgaaa catttaaatt cgaaaacttc aatccaatat
     1621 ctaaattaaa atcgattgtt tcatattcaa tcaacccaaa atcttgaaaa agaaaagaga
     1681 gggcatttct ttttatcaag cgcttctctc ttccagttaa atgttttgtt tcttgattaa
     1741 ataaataaaa ttgccctgaa ctgaattgat cattcaatcc taatatattc aataaagtac
     1801 ttttccctgc tccgctctct ccaactacag tcacaaaatc cccagcatcc gcagtgaaac
     1861 gatccaaatg aagaatcgtt ttgtttcgaa tgattttttt cgcacctttt atcactatca
     1921 acggctccaa ttgttaacct ccattcatca gcaataactg acttttcttt tctgtagaca
     1981 aaataaaaac aactgaaaga ataaaatcaa tgaagctaaa gaaaaaaata ttaaaaagat
     2041 atttaggctc tctttctata agaaaaagga agattacact tgtcgttccc catttgatga
     2101 ggttcatcat gaagaaatct ttatacgttt caaagagcga ataaccactt gaacgaaaga
     2161 tagcgatttt ccgacaattg tagacaaaat atatgcataa catgacatag ctcaacaaga
     2221 agaaagaaaa gaaagtgagt aaaaatatct gtatcaagta atgaaatgat ctgcttaaca
     2281 ctttgacagc tatctcttca gtctgagtat acggaaatga taactgcaag ttatcacgta
     2341 actgactttc ttcaatcagc tcaaataacc tttctttcga caaagagcct atcttgatct
     2401 ttaacggtgg atagatttct ccgctgagaa tatttctttc ccaatacgta ctgtttttca
     2461 atgtcagtat ttcaacaacc ggataatgtt cgatccaggg gatagaagga ataaatgtat
     2521 aaatgggttg attgtttttt ataaatagaa atttcggttc tttttcactg atttcttgat
     2581 aatattctgt tatttgcgcc aggtcatttt ttaaatctgt atgtctatat cgttcaggga
     2641 tcaatataac agggtgcgtt tctttttcgg ttatttggat caaccttcca gagacatcaa
     2701 tcaaaggaaa tttcttaaga taattcagat ttatttttat ccctctgcca aactgttcat
     2761 tttcttcatt caagtaatct gatacattga ctaatatact tccgttttta ttcaaagttt
     2821 catataattc ctgatcggct gctgatgcaa aacgattgga ataaataaaa tccacgtggt
     2881 ttttcccgat ggttactgga taaaatacat gatagtcctc accagcgatt tcttttggtg
     2941 tttcatggga aacagctaac tgtgaagaag aaaaaacgat agttgctaat tcgacattgg
     3001 caacggtcaa aagaagagaa aacaacaatc caaatgtaaa aagaatgtat tggatatagt
     3061 tgaacgcttc tcttaacgaa gcgatttttt caactaataa aagagaaatc aaagagatac
     3121 cctatattcc gctgaacaag aaaacgtaca tctggaacaa ataaagaaat tgctccttat
     3181 aatacataaa aataaaacca ccaatgattc ctgcaattat gaatgtaata atgattttct
     3241 ttttccccaa taaacaccac aatacttgat gtaatgaaaa gccattattc aacaataaac
     3301 tgatttcttt attcatcaat aagaaaaagg atgataacaa aaggaataaa agaatcaatc
     3361 caatagttaa atacactcga taattatata cgtctgtact tgaaataacc gaatacgaat
     3421 agctattttc aaagtcttca aaaatattag tggcataatc agtgtgaaat gcttgattga
     3481 atcgttccac aaatagtaaa atgaaccgtt cataggcttc tttttctctc gtcatcaaga
     3541 aaaattcacc ttcaaaatct tttattttga tcgattcttc aatggacgtt acgcgaaagg
     3601 aggtattaga atagggtacc ttctcgtgag aagatttgac atccccttga taatacaatg
     3661 tcatttcaag ctttggctgt atcttgaagt tccaagatgt atctacttca ttggattcat
     3721 ttactgtttt tttcatataa gaaagctcag tttctgatga gacattttca aataccatga
     3781 ttagctgctc atattcttct tgcccaataa aatgttgtgg caatcgaatc ggagtataag
     3841 tatctgtttg atggaaaaat tcatttgtcc gatagagtac ctgtttattt ataataacca
     3901 aggcaaaacc actcatgata ccggtcacta tgattgtgag taaaactgtg aacttttttt
     3961 tgattatcac cactctccct tacttaaatg attctttgat aaaatcatac cgtttttcat
     4021 caaaaaaacg tatctatcaa atgtataggt catgtagaat aaatctatat actgtagaat
     4081 cgaaaggaga agaagatgaa catcggagaa aaaatacgaa cttaccgcag aaattgtaag
     4141 cttacgcaaa aagaattagg cgaaaaaatc ggtgtttccg ataaaacaat ctctagttgg
     4201 gaaaattctc gtacaatgcc agaccttgaa atgctgtcac ttttacatca gactttaggt
     4261 ctgccgattg attttccttc tattgaatca gctaccgaat caatagaacc aacattgccg
     4321 gatgaacctc ttgttatcaa gaaatcttat tcatcaaaac aacccttctc actcaaaaag
     4381 acactatctt tctttctgct cgtcttttgt ttggtctatc tttttcttat caattatcaa
     4441 tataaaaatg aaacggttga tcgattcaat ccttttttga aaccacaaat cggctatacc
     4501 acactccctg taggatacgt ggaaaattcc atcgaacatt gggtatgtaa tgatatcttt
     4561 gttcatggta catggctgtc ttttcacgct ggagaggtag aaaaaggtaa gaatcatgct
     4621 gtaatcaaac acaaaggaaa acacgtcatg aacgttcgcc tgctcacttg ggaagccatt
     4681 ccctcagatt accaagaaat cctcatccaa gactatgacg atgccttaaa aagaagccct
     4741 caggagccag ataggcatta agtaggagat gaacgtagag atagaaaaaa tcaatgcaca
     4801 tgatcttatc aaaaaaaaac aggggtacaa attaattgaa aaagctttga gatgaaagtt
     4861 cctaaataaa aaagttcaga atgataggta aaaaaatgac taactattct agtattaggc
     4921 tatttgtatt attcttctgt tatttttata tcaaaaaatc cagaaataac taaatatagg
     4981 tatttctggc ttatagtata atatttacgc cacagccccc aatgattctt tttggaatca
     5041 tgatcaagga cggacataca caaaaatatt ttgcccattc gatccgaata gattaacgac
     5101 taatctagta agtaatatag attggtacgt agtcaaacca gcaaatggaa aagttgtcta
     5161 ttccacccca agggctttaa agacagcatc ttccggcgtt tgataaaaaa tcaaattaaa
     5221 ggtacccatt aattctgggg gtacagttcc aatatcagca gcgcttatca taggtacaag
     5281 tacttttttc gctccggcat cttttgctac ttgtaaacag ttagctaaat tatctacctt
     5341 aatcatagaa ccaccaattg taatatcccc taaaataact aaacttggta atacaggttt
     5401 atttaaggaa actgagcata aagctattat agttgataac gataatgttt ttgtcatacc
     5461 aatgccattc atatcttgaa catgaacgac aaaatctttt gtcgtcaaac taatagactg
     5521 actaacactt ccataattag ctcttaaata tcggtatgcc gaatccatcg cttctcttgc
     5581 tccagcatct gaattgatcc cagtacgttc aaactttcca tttcccgaag tcatttgcgt
     5641 ttccaattta taggtaccta attgcccagt tttgccataa gataccgtgt acaaatgccc
     5701 tggcttccct aacccttctg gaatcaaagt tcctccacct tgttcaggaa cagaaacata
     5761 ctcttctcgc tcttcttcta aatcaatgta tgaaaaattg acatcataaa attccattcc
     5821 accaattttc tttaattgtt cttttacacg acgacgcatt tcaagagcaa atcttagtat
     5881 ttcttcgaca tcactttttt caaatctacc atcaggatac aacagtttca tcatcccaga
     5941 tacaatccga cgaactgcaa tcgtatcccg ctgatttaat tgagaaccaa gtttgaaata
     6001 tttatcaaaa ctatcgccaa aactaacttt tcgaagctca cgcatgactt cagaaaaata
     6061 atctgtaatg aagccatagt catttgtaaa aaaatctggt cgatatttag gaatttccca
     6121 acctggtaaa taacaatgca tccgatccaa aaaggctgaa tccgtcccca taacctccgg
     6181 aaatggctca aataaattcg aagttttcaa tattacatca acgctttgat taatatttcc
     6241 tacaaaagcc attgatgctg aagcattctt ttcttctttg cctctagcaa aagatcctga
     6301 agccatgtaa tctttcatga tttgaacgcc atcattatcc ttaaatttaa tcccagcaac
     6361 ttcgtcaaaa gcaacacagt cccacatacc tacaagtccc attgtacgac tagccatatt
     6421 atagaaaagg tttgctactg ttgtttgtcc acctgaaaca aggattgagt ttggtgaaat
     6481 ttctttatat acatgcgatt tacctgtacc acgtggtcct agttcacaga gattaaaatt
     6541 attttcaatt aatggaataa gtcttgctaa atgaagccat tttacccttt tattaaattg
     6601 tgtaggttcc ataccaatgg accgtagaag taagtcaatc cattcatttg tagtaaactc
     6661 cttgcgagcc atcttgtaag cctcaatgtc taaaccaggc atttggattg gatcaacctt
     6721 ttttgcaata aaagggctat tccaacgatc attctcatca taccaatatt ccatcgaaac
     6781 aatacaccag atccccccac ctaataatcg atcaaatttt ttcggatact cttcactaat
     6841 ttgaacattt tttaatccca ggttagagaa ttctgcttca taacaatctt tcttttcatt
     6901 taaatgtaca gttactttat caataactgt ataaccaccc aattcacgaa tttttgcttt
     6961 tactttttct gcttcatcag gacgcacata gtttcgagca agaatatttt taacattttc
     7021 cacacctttt tcaaccaatg tttcatccaa tgacgaagca tacataccaa gtaaatattc
     7081 caatacataa acaggtacat tcgcaccttc tttaattttc tttgttaaat cttttctgac
     7141 aatctttccg ggaaagaatt cagataactt taaaatttcc tgttgttcca ctagaattcc
     7201 tccatgtgtt cagtcattat aatagcaatt aaagtatatc atacttaacc agtaggcttg
     7261 ataatacaaa agaaaaagta tccacaaaaa attgtggata cctcgttttt atatatcaaa
     7321 atcaaatgat tctgcaaaag gattatctat aatgaatttt attcgctcgg tcaaatcatt
     7381 tttttgattt tttatcacta aataataagt atcttcaact gtatatcgac gatttacaaa
     7441 ttcaaaaaca aatttagtaa accgctgatt tgcatcttct gtttttgaat ccgcaacata
     7501 gatttcttca tttgagatac gttcaccata ttcatcttca aaataaagcc agaaagatgc
     7561 ggggtcataa acatcactaa ttgcattttt ttgatagaat tctaacgtgg ttgataatcc
     7621 tgtaattctg cggttgctag tcattaattc aattgctact ggttcctttg cattgctacc
     7681 tttggctacc gtaatttcta gaacagggac tagcatttct tgaatagatg accctccatg
     7741 aacgtagctt tgtcccccgc cagctttctt aaaaatactt gaagtaatcg ggacacaaat
     7801 gtttctagtg tcatcatttc ctaaagaatc tcccaaagag taatttttcg taccaattat
     7861 ttcgtattct tcttcactaa gaataaaacg acgttctaca cgatcttttt catttctaga
     7921 tggattttca atcttatcta cttcttcaac gtggcttctt ttataaataa atccatgatc
     7981 agccgtaact ataaaacgat agatactgcc atcattatgc aagcgattaa taagtcggga
     8041 aatttcgctt atcgctaaat cacaggcctc aaaaactcga ttttcattcg agtgatcacc
     8101 tgttctatcc acttgatcgt gataaatata aatgactgat ttgccattaa atacttctcg
     8161 tacctcagcc ttattcatct taaccacatc atcataattt aaagctacag catttgcatc
     8221 tcgttgctgg agaatcgcat ttcgattgga taaaccattc gtttttttcc catcaatcag
     8281 tacttctgtt ccttcattgt aagttagttg attgtttggt aataaggcgg ctttaccgaa
     8341 ttcagtaact gaaggtaata cagaaagaaa gctagccatg tttgttgaat acttcttttc
     8401 tctaactaac ttacgttcta aattcatccc taactcatat cgaaaagcat ctgaaataat
     8461 aactacagtt ttaacagagc gtttcattac agcttgatta taaaattttc gaatggatgg
     8521 acgattctta aattcaaaag tttgattcca aatttgtcct gattgatcaa gaaacttcga
     8581 ataatttgct tcaactaatc gatacaattg gccatatttt tgcttttctt ctatatgatc
     8641 actattccaa ataaatttcc gataatgcgt atcaacccaa tacatttcct tctcatattg
     8701 agttagcaaa ttatccatgt tcatcggcaa gcacaatctg tttttatcca caagataata
     8761 agcatgtttc aataattgat actcataaat ataatccgat gcgaaatgca ttttttcgcg
     8821 ctgctttaaa ctatcaaaaa ttgtttgtcc tgcaattttc gttgtagtgt cagaagcctt
     8881 taatcgttct gcataaaaca ttaatatttt tgcatgaatc tcagaaaata cgtctgcttc
     8941 aattagatca tcaatttcta tactgtctaa taatccatca ccatcaatag cctgatatac
     9001 ttttttagat agctgttcaa agctttccat gtaacgtgta tcattcataa actgatccat
     9061 gaaagtaata atgtttgctt tcaaatgtag tatttcaaat cctttgaact tcttcggcat
     9121 ctcttgatca atttgataat aaaaatagtt caaataaaga cacatcgtca gcttttccaa
     9181 tgttggctgg ctatccgtaa agccaaattt catttgaata aattcccaaa atttagctgt
     9241 taactgatac ttatcaagtt cccttaaagg attctcccta ctttctgtaa agctttttag
     9301 aactacacgc agtaaatcat ttaatcgaat acttttcgat ttcaacaaaa cagccattat
     9361 cattaattca atcgtttctg atttattaat tgtcatatgc gtactaaatt caccaaaact
     9421 tctcatgcga tcctttgcat taaagaaatt acgatactgt ttaactgtct gatgaaatct
     9481 aagtggaata gccaattcat tcatgataat cgctaagcgg tctgcactaa attcctctga
     9541 ataattcaat acactcaaca aatgattatc ttcatcttta tcatcccaac gaggaaatgg
     9601 cgcatagatt aaatagttag tttcgggatc ttcatattcc aataaacgct tagttttaaa
     9661 ttgtgcgtcc ttttctaatc gatagatttt tgcattttga atgaccaatg agtcaatttc
     9721 ttcaagaaaa tcctgatttg aatcaaacca aaaaatgatt ttcctacatc cataattagg
     9781 aaaacgttga tttaattttt cttcaatttg ttgcgtattt atatctgtca tcaatctcac
     9841 ctaatcgttc tctttctata ccccttatat tgacataatt tggataattt cgcacgcaat
     9901 ttaagaataa aaaagaaagt tgttagaaaa caactttctt agataactat ttttttggtt
     9961 ctttgacccc ttttgcaaaa atttggaatt tctcgccggt tttaggatca cgctgcaact
    10021 tatcataatt gacaattaca ccatcatcta agtctaattc aattcgtttt aatgctaagt
    10081 gacccaactt ctcgtcatac tcacgaactt cttttaattg cttctgaatc tttacaattt
    10141 cttttgtcat tgcatttctt tcttttgggt tatcacttgt tgaaatttgt tgctgacgca
    10201 attcgattcg attgtcatac gctttttgta gttcgtgtag gtattcagta cgtacttttc
    10261 ctaattgatc tggtgtatag cgatgcaagt acattaacgc tttaaagcca ttttgttttc
    10321 ctgagtccaa ttgccaataa attggtcgat tggagtatgt ttttaaatgg tctttgtaaa
    10381 aatcttgtac gaagtaacga cgaataattt ctcgacttgt ccctttgcca cctaaagtgt
    10441 cagcgataaa ctggagattt tcttcaaggg tttctttacc aaaagtaatc tcaacgaaat
    10501 caatgaaacg attgacaatg tcaatttggc tatccttgaa atactcatca tcggtgatga
    10561 gtaaaacatt atcagtatca ggtttaaatt tttgatagcg ggaaggatcc cattcgccgc
    10621 cagcgaagat gagaccatct tcgtctaggg agtaacgacc gaagatacaa cctattgaat
    10681 aagaaataaa atccttaatt tccctcgcct tatcaggtaa ccttgcagag atatattcat
    10741 cttgaacctc ttttgttaat tctttttcaa gagaataatg attaatgaat atgtcgttca
    10801 gttcctcttc attcgatttt agagtattaa attgattaac caactgccta cttagacagt
    10861 taaatgcctc tgagagatct tttttaaaca tataacttaa caaaatgttt cttctaaact
    10921 cgtatgacgt ttcaagagca ttatagtctt ttctagctat atcgatattt tcatccactt
    10981 tttctcttat tttttttaat tttctctcag atgtaacttt aagaaaaggt agttttccca
    11041 ttggtccttc atggtaatcc aaggtgggag aaataaaagt taaaaagtga ttaactaatt
    11101 tagagtttac atagccaaat atttcttcaa agtcagttat atcctttgag aacagaacag
    11161 atcctttagt ttcaaaaatt gcctgattct gcaatctaaa agatacttgt ccagatgaaa
    11221 gtgtcgacca agtaattccc tttttaaaat agtaattaag attttgtggt ctagatttta
    11281 acttcccatt ttcattaaca taatttttta tttcatctcc atcattctcc cagttaacca
    11341 cgtattcatt gtttccatac cattttctaa aacgaccacc tttagttgta ggaaaccatt
    11401 tttttctaga ctttttagcg tcctctaatg ttttagcatc aaactttatt ttgttaaatt
    11461 ctacttcaaa ccaataccgc aaaaatttat tgttatttcc ggttgccaac ccttgttttg
    11521 catctgcaac atcgttagtt aattttcctt gagcaaaatc agtgattacg ttatcgcttg
    11581 cccagtaagc aatcggcatt cctgggattt ttttgaagtt gtcttgatta gtactataaa
    11641 aataatcgca ttcaccatgc tgatgaattg cttctaacgt tttaattttt tgaacttcca
    11701 ttccaccttt aaaatctgat aattttatat agcttccatc ttgttcattt gaatttttta
    11761 tcgtaaacgt acaaattgga acggttgctt cttcaaaagc tgaatactcc atttgaatca
    11821 aactagaaat gtttctttca ttaattaaaa atgagcgtaa cttttcatag ctagaaatga
    11881 acatccaaac aaatggcgtc ataaaggaag catatccatt atcaatcagc atttggctat
    11941 tcaatttaat aaacacagaa aatagatctt ttttgacatc agaatagtta tcatttacat
    12001 atttttttaa tttcttatcc attttattaa gataaggcgg attagtgact accgctacat
    12061 atttatcaac tagcatttca gccacgtcaa gaatgtgcaa gtatttttct tgtatttcct
    12121 tcatcccata aagaatatcc atattcgtaa attcgttgaa ttttgatata tactctctta
    12181 cttttcctaa gtattcttta gttattgcct gattattttt atcgactaac ttaatgattg
    12241 atccaagttc tttcccattt tcaaattgat caaaaattaa ctctttgtat tcattccagc
    12301 gttcactata cttatcattg ttcaattcac taatacgctc ttgatactcg tctgtcactt
    12361 taggagaatc aataaactct cttaaattcg gcgatagact ttttttcaaa aaaagacgat
    12421 cgtattgacg ccctttcatc ataattgcaa aataagacaa ttgatacgct cttttatcga
    12481 tttcaagacc atataaattg tattcaacga ttgcacgtgc agcatcacgc tcagaatatc
    12541 ctaacgtaag ataaatttcc atcaatacat cgaaggcgta aactaaaata tgcccagacc
    12601 ccattgcgtt atcgataatt tttagttctt ctggttttaa attacttaca gatgttgctt
    12661 gttcaaaagc atatttatct ggaattaagt atttcaactt atttcttaat tcactattgg
    12721 gataatgatc taaccaatac tttcctaaac tattgtcaac catataacga acgacccaat
    12781 ctgtggtaaa tagttgagtt gctgccggaa tatcttgttt tttgacaatc cctttataaa
    12841 tattcaccac ttgattatgt ggctcactat tatagtactg atacaaccac ccaataatct
    12901 ctacctgtcc accagcttga acatagaaat catcctctgg aatacttaaa agatcagcga
    12961 ttacaccatc ttttttaata taagaagctg taaacaaaag ttctgtataa tcttctactt
    13021 cttcaaatag ctcaggtaag taatcattca attgattaca gatttttata aaaatcaatt
    13081 gatacagctt gtccattgct tctgctgaat tatctgtttt ccaagttctg acttgttgct
    13141 gttcattact agataattct tcaaagatat cggcatcata aatatctgta ataatatctg
    13201 gctcaatttt cccacttatt tcactagata atacacgata cgtgtcaggt aaataatcat
    13261 ttacctccat aaaccgaatg gcaatcaatc ggttaaacca cgtataagca acctcttcga
    13321 ttaactcttg aaaagcttct gaataagaag tgacagaagc acgttgtttt agttctgcaa
    13381 tcaatttgtt tctttgcgct atttcttttc cttgaattct tattcctgat ttttcgattt
    13441 cttgtaaatt gctatcaatt ttcttcacat cttcaatttt tgattcatca attcctaaac
    13501 gttttgcttt agtcattact tcatcaatta attttctacg ggcattgacc gcgaaattct
    13561 ttatcgcttt cttatccatt tttctttccc ttctatatgt aacctttgaa aagacaggca
    13621 cctttttatg cctgtctttt cactagaatt gaatcgtgat ttctgtattt tcttgaagtt
    13681 gttccttcag tttttgatta atttcagctg ttaactcatc aatatctgct gctgacttaa
    13741 tagttttaac atgaacgtta agtgttctca ttggtactgt tttctgttga atagtaggtt
    13801 ttactatcgc tatttcttca gcttttgctg cagagtctat ttcatctttt tcagtttcac
    13861 gaatcaagtt tgctgcttca atttgtttac gtgcttgttc ttcttgacgt ctgcggtttt
    13921 cttcattatc gaaacgttga ttaaagatca aatactgttc tgctacgttt aatgataaga
    13981 aaacaagatc atctatagta ttcgcttgtt caatttttgc tttcagttct tgaaattctt
    14041 ggtcaactat tggtacaaat tgatccttaa aatcttttcc ttctaataat ttaaatgttg
    14101 cttgtttttc ttccgttaat ttatttaata tttcctgact ttccaactct aacagttgat
    14161 tatattgttc aataaattct tgaatcaact catttagttt catcatatct gaataaggat
    14221 cagccttttt caaaatggtt ttcatctcat gaatcaattg atcaatttct tggctagaag
    14281 tttgacgccg acttttttcg attaatctaa ttttatcttg tgcattctcc cagatttttt
    14341 tctgagtact attttgcttg tggaaatttt taattttatc atatgtttca gcccagtcga
    14401 ataattcatc gcgatatttt ttgattgtat aaaaaaagtc ttcaacatct gacgtttgaa
    14461 caattacttc aagatatttt tttatttgct catattcata agcactaggg taggtataag
    14521 tgttttgtaa atcaaaataa gagcgtaaat tgtctaattg accttttaaa tatttcttga
    14581 aattttccat tgtatgttca ttgttatttt catcaacaat attacgactt tgaaacagtt
    14641 ctctaccgat ttcgtcaacc aatttttttg cttttggcga aatcaccgcg cttggttcaa
    14701 tttttatctt ttcactttct cgtcgttttt gaataatcat tacaatacta tttggatcat
    14761 ccatccgtga aatttgtaca ttgttataaa gtaaatcaat tttaccagac atgaaaagtt
    14821 tagcgatgca ccattccaca tcaagttctg tgaatccata cggttgtttt gtttcaaaac
    14881 gatcacgaat tgttttcatc gtaatcgtta ggtgatttct cttttgatca ttgataaaat
    14941 tgaaaatttc ctgaactgct tgctcatttg atggatcttc aataatttca tttttgcttt
    15001 ctttcactaa atataagata tcttcttttt ctttaggcgc atcaatttgg tttaatttat
    15061 ggtatacttc ttcagccatt ttctgaagtg ctgattggaa ttttgcttta aagtcggagc
    15121 ctgtcagttg aatttctgca ccgttataga aaaattttgc agatttcaac agttcattta
    15181 attgatcgtt gatacttgca cttagttcct gtaattcacc agatttttcc gaacgaatcg
    15241 cgtcaaagtt ctttactaca ctcgaataag tcgttgttaa ataaagattc attctagcat
    15301 acattgctat atcatcttta aattctttat ctgatggcaa ttctacgaat aataagttac
    15361 gggaactctt aagcgcaagt tctgcctctt gacctgctaa atcagagtat ggtgaaataa
    15421 aattaactgt catttcagca tttccagctt gcttgtatgg acgttgatct aacacttgtg
    15481 cataagtaaa cgtataccgg tttttaaatt tatttccacc tgacatgcct aatttttcat
    15541 aggcaaaact atttcggtct agaacgccgt caaaaattcg tttagatagt tctacgatca
    15601 aatcactatc tgtaatattt tgatttttaa tctcacgagt aatttcctgt tcttcatcag
    15661 tttggaaaat ataactatct aagttcttcc gcacaaagtt ttccttaatt aaaaggtcca
    15721 atgcttcttc tactcgtttt ttagtttcca ggcggtcttc gttgatattt cttatcatca
    15781 agctcgtaat attttcaaca tttggtttga tggcttgatc aacatattta atcatgaata
    15841 aaattcgtaa gacatttacg ttaaagttat ctatatcagt tggtctttga atatgagaat
    15901 tatttgtagc ttgtgtaatc actcggctaa ttgctgtatc aatccaacgt tctaacgagt
    15961 aataaaactg atctaaagaa accaaactgg ttgtatcatt ttccttattt cgttcagcgg
    16021 cttctttaaa cattgctagt aatgaacgtt caccagacga taagtttgat ccttgatagc
    16081 tgtgattacg aatctcggtt agaatttgcc ccaataaatt gaattgatag ggaataaatg
    16141 gataaacttc tgcgtaattt tcagcatctt ggaagagctt taattcagga ctatcaacaa
    16201 agagtaattt atttttcaat tctgtttgaa cagtttcaaa ttgtaaggct aaagaatcat
    16261 tggcaaactc ttttttcttc aaaatacgtt ctttaattac ttcatcggca tttgctgaag
    16321 ataaactcac agttgtggca aaacgatcct tgatcttaga aaaatcatta tcagaattgt
    16381 tatcaaagtt tgttacgtga tcaatcgctt cctgacttgt cactaatacc caagccttac
    16441 cttcacaaat tcgacccaaa tcttccacaa cggtttgtaa ctgcaaagtc aactcacgat
    16501 taccgccgat atattgtccg acctcatcga ctagaaaggc aatatgatgg tctgcttctt
    16561 tggaatcaag atagctttta actagatttg caaagtcatc aatgcttatt aaataatttc
    16621 ctgatgcttt ttctaaaaaa ttatcaatag cggcttcact cataaaatcg attttaagtg
    16681 ccgcttcttt gatatcatct tggatccaat caaactcatg gcgctcttct tgccattttg
    16741 ccccattaat ctcttcaaaa gcagctttga atgcatcaaa cttttcttct ttaattaacc
    16801 gacgttcaaa atctgctaga tgtggaatcg tgcttaatcc ttgcatttcg ttaaatacct
    16861 ttaaaaaaac attcacgatt ccgtttttgt cttttttacc acctgcatca ctttttgaat
    16921 caatattaaa cagaataaca tctgtattta tttgtgagca ccgttccatc gttgccaaag
    16981 tcagtggatc tttgattttc ccatcttctt tgaagtaatc aatggctttc ttgtcattta
    17041 tttctcgatt tgcgagcaag taagaaataa tttttaagaa atgagattta cctgaaccaa
    17101 agaacccctg aatccaaaca cccattttat ctgttggtgt attgatactc cgttggtagg
    17161 catcaaaaat tgttttaaag tgtttttgta gttctttcgt aaccacgtat tcttctaatt
    17221 cttgttgaac attttcatcc tttgcttgac cgaccttaat aactccttga atatttcggt
    17281 caattggttt tgcaaaaata tctttaatta gcatttaatc tcctaccttc catcccaact
    17341 aaattctaaa tgctcgataa taattatcat ctttattggt attaagaatt gtcaaactta
    17401 attcatcata ttcccctgga tacatcatca caactggtac gcgatcaaat acctgatgca
    17461 ttttatttaa aatagagtgc gctctaatta atggatataa ttcgccaatt cctgtcagga
    17521 acacaactga tttttctggt gtatgggcat ccacatattc aaaaaataca ttttcttcct
    17581 caggtaaatc cattttcaat aaacgaccaa tgctttccac taatttttgt aaaccacgtc
    17641 gttcttccat cttaatcgct ttttcagtga aaccgcgttt ttctagatac tctaacataa
    17701 tcacatagag attataaact ttcacttcat aattatcacc aacagatgtt ccagatatat
    17761 cattaatata atctcttaca attaactcgt ctttcggatc gtagtcaaag atataatagc
    17821 ctacttcatt acctaaacca cgattcaata aaaaatcttc tttttgaatt acttcattca
    17881 acttttccaa atgctcttga atcgataatg acattataaa attcctccca gtgctagtac
    17941 aatatcttta cgattcttgt caactaacca ttgaatcaat cgtttatcta ataatatcgg
    18001 agtcattcgt ccatattctt tttcccctct gactaatcct gtttcaatta gattcgtttg
    18061 ataagcggca ataagccgtt taatcgtata atctttccat tttgaaatat cttctgaact
    18121 tctccgcttc tctgtaaaaa aagaacggta atcaactggt gttaattgca aatttccttt
    18181 acgtagattt tcagcatata cctcagtcat aaattcatta aaaagatcat caatcataag
    18241 gatggaaatc aaattcacta acttttgatt atctatattt aactgattga aaagttgcaa
    18301 tccagcttca tccagttgac taactctcct tttcacgttc tgatagattt ctttttgacg
    18361 gttactgctc actgcaaaaa aaatattttc atgttcattt ttctctttaa tttctatatc
    18421 tgtatatcct tctagtcgta attgaatata taacttgaac tctgaaaacc aaaagcgttt
    18481 agaaactaga ccagcagaat attttttatt caatagattg cctcctaatc atctataatt
    18541 caataagtaa acaagtgttt tttgctcatc gttagcattc attttttagt atctactata
    18601 caatacatat ttattatag
//
LOCUS       DAZKOR010000055        18582 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000055 DAZKOR010000000
VERSION     DAZKOR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.055"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..237
                     /locus_tag="ACG8ZM_001792"
     CDS             154..237
                     /locus_tag="ACG8ZM_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0492747.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            362..1099
                     /gene="erm(B)"
                     /locus_tag="ACG8ZM_001793"
     CDS             362..1099
                     /gene="erm(B)"
                     /locus_tag="ACG8ZM_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0492748.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1362..2225)
                     /locus_tag="ACG8ZM_001794"
     CDS             complement(1362..2225)
                     /locus_tag="ACG8ZM_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0492749.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2227..2499)
                     /locus_tag="ACG8ZM_001795"
     CDS             complement(2227..2499)
                     /locus_tag="ACG8ZM_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0492750.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2516..2725)
                     /locus_tag="ACG8ZM_001796"
     CDS             complement(2516..2725)
                     /locus_tag="ACG8ZM_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0492751.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2824..3720)
                     /locus_tag="ACG8ZM_001797"
     CDS             complement(2824..3720)
                     /locus_tag="ACG8ZM_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0492752.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            complement(3890..4383)
                     /locus_tag="ACG8ZM_001798"
                     /pseudo
     CDS             complement(3890..4383)
                     /locus_tag="ACG8ZM_001798"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4459..4590)
                     /locus_tag="ACG8ZM_001799"
                     /pseudo
     CDS             complement(4459..4590)
                     /locus_tag="ACG8ZM_001799"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(4866..5660)
                     /locus_tag="ACG8ZM_001800"
                     /note="aph(3')-IIIa"
     CDS             complement(4866..5660)
                     /locus_tag="ACG8ZM_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFQ0492753.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(5753..6295)
                     /gene="sat4"
                     /locus_tag="ACG8ZM_001801"
     CDS             complement(5753..6295)
                     /gene="sat4"
                     /locus_tag="ACG8ZM_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFQ0492754.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            complement(6292..7200)
                     /locus_tag="ACG8ZM_001802"
     CDS             complement(6292..7200)
                     /locus_tag="ACG8ZM_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0492755.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            complement(7233..7967)
                     /locus_tag="ACG8ZM_001803"
     CDS             complement(7233..7967)
                     /locus_tag="ACG8ZM_001803"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0492756.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            complement(7948..8817)
                     /locus_tag="ACG8ZM_001804"
     CDS             complement(7948..8817)
                     /locus_tag="ACG8ZM_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0492757.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            complement(8832..9056)
                     /locus_tag="ACG8ZM_001805"
     CDS             complement(8832..9056)
                     /locus_tag="ACG8ZM_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0492758.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            complement(9199..10743)
                     /locus_tag="ACG8ZM_001806"
     CDS             complement(9199..10743)
                     /locus_tag="ACG8ZM_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0492759.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            complement(10763..11179)
                     /locus_tag="ACG8ZM_001807"
     CDS             complement(10763..11179)
                     /locus_tag="ACG8ZM_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFQ0492760.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            complement(11180..11677)
                     /locus_tag="ACG8ZM_001808"
     CDS             complement(11180..11677)
                     /locus_tag="ACG8ZM_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFQ0492761.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDC
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            complement(11988..12665)
                     /locus_tag="ACG8ZM_001809"
                     /pseudo
     CDS             complement(11988..12665)
                     /locus_tag="ACG8ZM_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(12665..13282)
                     /locus_tag="ACG8ZM_001810"
     CDS             complement(12665..13282)
                     /locus_tag="ACG8ZM_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0492762.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(13296..13466)
                     /locus_tag="ACG8ZM_001811"
     CDS             complement(13296..13466)
                     /locus_tag="ACG8ZM_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492763.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(13814..15304)
                     /locus_tag="ACG8ZM_001812"
     CDS             complement(13814..15304)
                     /locus_tag="ACG8ZM_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0492764.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(15347..15646)
                     /locus_tag="ACG8ZM_001813"
     CDS             complement(15347..15646)
                     /locus_tag="ACG8ZM_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492765.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(16012..16296)
                     /locus_tag="ACG8ZM_001814"
     CDS             complement(16012..16296)
                     /locus_tag="ACG8ZM_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0492766.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(16815..17300)
                     /gene="vanZ-A"
                     /locus_tag="ACG8ZM_001815"
     CDS             complement(16815..17300)
                     /gene="vanZ-A"
                     /locus_tag="ACG8ZM_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0492767.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(17453..18364)
                     /locus_tag="ACG8ZM_001816"
     CDS             complement(17453..18364)
                     /locus_tag="ACG8ZM_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0492768.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         5332 a         3746 c         2839 g         6665 t
ORIGIN      
        1 gattcatcgt cattcctcct gctatctttt tctattattc taccttattt gatagtagat
       61 ttaaaacttt gcaacagaac caaaattttg tataatagga attgaagtta aattagatgc
      121 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgtaatgta
      181 gataaaacat ctaccgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      241 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      301 acaaacaaat cgtttaactt ctgtatttat ttatagatgt aatcacttca ggagtgatta
      361 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      421 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      481 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      541 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aacactcgtg tcactttaat
      601 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      661 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc
      721 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
      781 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
      841 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
      901 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
      961 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1021 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1081 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1141 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1201 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1261 aaattcctcg taggcgctcg ggaccccaac atgaggaaat gtttttataa aaaaagagta
     1321 gttaccgttt tttggtaact actcttttcc ttttttaagg tttaaatacc tggaagttta
     1381 ggtgttttgg gtattggtgg tgtaggtagc tgcaagcttt ccattttttg ttgaattgct
     1441 ttaaattcag gggtttcttg gtgttgattt tgaaccattt tttgctctat tctctccaaa
     1501 gtcggttgaa tttctttccc tgatacttta cgatttaatt ctctttctaa ggtctctttc
     1561 ggactaatgg aaggcgtttc taagcttgaa tagagtttca ctccttctct attgtaaagc
     1621 cttatatcgc taaataagcc cgttttatga agggtttcta aattggtcgg taagtttttg
     1681 acaacaatat catgcgcttg ttttggtgtt gccctagctg tcattggatc atctgcatac
     1741 atggtttcat atcgttcaat cgttcctaag tatgattcaa ttttcggtac tgccatgacg
     1801 tatatttttg tttcataacc tttagattga agcattgtgg ctgtttgaat gggaacgtct
     1861 gttgttcgtc ctgttccttc aatcactaaa ttatagcctt gatcgcttaa acggctaata
     1921 agtgcttctg tcatgcgatt cgaataaggg gtaacgtgtt ttactacgtc tttttcatag
     1981 agtttcacta attcgtcaaa atttgggtgc tgttgtttaa aggtatcatt atcaatgaca
     2041 ataacattcc cttgtgtttc ttcaaaaatt gctgatcgca aactggtttt ccctgaccct
     2101 ggttgcccac caagtaaaaa agcggttggc gattcaaccg ctagtctatt tttagttagt
     2161 cgttcaacat tcttttctaa acgatcttca aattgctttt cggtgaagtc agtaatattc
     2221 gccatgttaa gccactttct ctttattcaa aatttgtttt gaatattgat tcattgaccg
     2281 ccaatactca tgaatagctt gtaattcttc aaatggttgt tgaaataaat caacttcttg
     2341 tgccacttct aattgattta aaagattcac ttctagtaaa cgagtatttt tagtatctag
     2401 ttcatgattc aaaacataat ttaatacccg attataaaca cttgccgata actcattaat
     2461 gatttcaatt tcaaatgttt tttcatacgt aactgccatt tcattttcac tccttttaaa
     2521 gtttgtcagg taaatatttc cgtatatatt cttctaaggc ttgatccata acttctttta
     2581 cgtttccgcc attctttgct gtttcgattt ttatgatatg gtgcaagtcg gcacgaacac
     2641 gaaccgtctt atcccccatt atatcttttt ttgcgctgat tggcgtatca tttcgttttg
     2701 ctttctgtgc gcctaaattt cccacgatcg ctcacttctt tctatttctt cttactattt
     2761 attttatcat cacctatcac aaatcacaag tgatttgtga ttaatcactt gtgatttgtg
     2821 attctattct ttttcatttt ctaattgaat gattcgctca atcatttcaa aaaatacgtt
     2881 ctcatacata gacaaaactt ttttgtcata gcctttgtgt tctgtaatgc catttttaga
     2941 ccaagtactt actttattac ttcgcttgat aatatttcgg aaaaccaaat tatcctcttt
     3001 atgttctttg tacagttctt ccaggtttga ttttatcgtt gcgctgtccg tatcaactaa
     3061 ataaggaaca aaaccaatca tatctagtcc agggttaaac tgttcttgca aatcaatcaa
     3121 ataggaaata tagttttgaa tgttgtttgt actttcttct tctgcttgta aagggatcat
     3181 aacgtaatca ctcgccacga ttgcattatt tgtataaacg cttggcgttg gtacagtatc
     3241 aataataata agatcatagt cactttttaa aggtgctaaa agagtagcaa gcaatctact
     3301 ttcattttca aacgtccatg agcgagttaa ttttggcagt aacatcaaat caaacgtgcc
     3361 agggatcaag tctaaattat cagtcaaatg aataatagaa gaagccaagt ttccattttt
     3421 caagccttca taaaaattga cacgtggcaa ttctacctca aaagtttttg ctaagtcttt
     3481 tgtcaatgtt gcctgtaagt ccttatcgat cattaaaact tttaaattca atttgtctgt
     3541 caagtaagca aacatagtcg ataatttgga ctttccaaca ccacctttaa agtaattatt
     3601 caagataata attgcttcat ttttattgtt taaaatacgt cttaattctt caagtatttt
     3661 tagtttttcc ttctccataa ctacgcccca ttcttttttt gtatagtaat attgtatcat
     3721 taagaatcac aaatcacaag tgatttgtga ttaatcactt gtgatttgtg ataagtgatt
     3781 tgtgattaat atataaaagc cctctttaaa gggcttttat atttattttg agaaagatat
     3841 aaaatcaata tatccctttt ccccaatttt tacaacggca ttgtagggca tatagttttt
     3901 tatatgaaag catttgtttt aatatttgtt ccaaagagcc atcatggctt tgacagagct
     3961 cttcttctat gcaaatgctt ttaatccgat ccacacgaaa attcctatat tcatttctta
     4021 gcctgcaaaa ccgattaagt accagttctg ttcataaaac cccagtgata taggctctat
     4081 cattctgctt tccggttctt gccctcctga tgcaggatat tgaattgata ttaccctttt
     4141 attgcagatt gcagtctgaa ttgctgcaat aacgttatcc gccaatgtat ttttctgtcc
     4201 agatgtgctg tacacgttaa tactgttctc caactcctgt gcatagtttt tttcaccatg
     4261 cttcaaaata gatttgattt tatacatagc tgaatcaaaa tcctttataa atgattcatc
     4321 tccatggcaa tttaaaaatt tccctgctgt aatcaatgca cccacttccg ccgctgtaaa
     4381 cattaccggc ggaagtagga agccctctac aagaaaatat ccttttccgg cctcagatcc
     4441 aattggtatt ccggcttctt caagtgtccg gatatctctg taaactgtcc ttaagcttat
     4501 attaaatcgc tgtgcaattt ctttggcagg aattattttt tttgattgca gctgaataag
     4561 aatagaggtt actctgttaa tcctgttcaa attttattcc tcctctgtga cttccataaa
     4621 aagtatatcc tatgcgttct taagctagct gctgttagtg cttctcattg caccctggtt
     4681 ataaaagcca tactcacaaa cctcttcacc aaaactcttt acatctctaa tatagaactc
     4741 tatctagcga acttttagaa aagatataaa acatcagagt atggacagtt gcggatgtac
     4801 ttcagaaaag attagatgtc taaaaagctt gtagttaaag ctttttagac atctaaatct
     4861 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     4921 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
     4981 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
     5041 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     5101 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     5161 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
     5221 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
     5281 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
     5341 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
     5401 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     5461 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     5521 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
     5581 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
     5641 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
     5701 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     5761 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
     5821 cgacggagcc gattttgaaa ccacaattat gatagaattt acaagctata aggttattgt
     5881 cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct atagatatat
     5941 tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacggcg atatcttcta
     6001 tataagcgta ccggttccaa ttttttcgca gtttaacttt tccgacgcat ttatcgtctt
     6061 ggtagtaaag atatattatc ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct
     6121 catcctcttc atcctcttcg tcttggtagc tttttaaata tggcgcttca tagagtaatt
     6181 ctgtaaaggt ccaattctcg ttttcatacc tcggtataat cttacctatc acctcaaatg
     6241 gttcgctggg tttatcgata tctttcaggt gccctgcttt catttctgta atcactgttc
     6301 ccgcctctct tctatatcag cggcatatgt gctatccagg cagccggttt taccagtgta
     6361 ttttttatac atgtccctgg tatattttgt tatattccta tcatactccg gataggcata
     6421 atgaagcctt tccgccacct caccggatac cgccctgaac aattgatggc atagaaataa
     6481 tgcttcccat atgttttcat aggaatccat ccggtaggtg gacaaaagtt tctcccacaa
     6541 atcctcggat atataccttt caataaactt atagttcttg cctacactta atttaaagcc
     6601 tgtttcgatg ccgaccttcc atgatatcat tctcagcagc tcatggcgaa caatctgatt
     6661 aaaatgatca atagcaaata aaatttcctt acggcacaat cctttaataa cataaggtgt
     6721 tacattccaa aattcattgc agcaatcatc atactccctt gcgcttggct ttcttacatg
     6781 ataatctata tcagtcggaa ctatgtccct tttaattcta caatctttat caattagaac
     6841 ctttattaat ttatcgccct ttaggtaatt atctaactct tccaagggca ataaggtaag
     6901 atcaatttta ttgtaatcat caaatagcat aagataggaa aatccctttt cttcaggtgg
     6961 gaataattcc atatcctccg gcttttgcat cattattata ttcccaaatt gattaagcca
     7021 gtcatcatta gatataaacg gttctatatc acttacaaaa tatgtaatat cataatcctg
     7081 aaattcatct ttaggtatat taatatttgc gcgtgacccc tcaagggtca caattcgaat
     7141 acgttcatcc tgttctgcta aagaaagtac taaatccatc atttcttttt ctgatctcat
     7201 ttacatactc ctcgtttatt ttttctatat tattacatct tcttttttgc cgatacaatc
     7261 agcatcattg ggcgtcgcat ttcatccgcc atccccggaa tatccatcat gttctctggc
     7321 ggctgtggct ccacaatctg atttattata aaactatttg aaagcagtgt atttagatat
     7381 gtggtcagtg ttctatgata ttttgtaacc ttttcttcca aaaacatagc tgtccgtttg
     7441 ccctcataat aataattgtc caccgggaaa tgcagtattt ctcctttttc gttataatac
     7501 cagtcttgtg ttccatgagc agtaaaaaca ggatgttcaa ctgtaaaaac taaattgcca
     7561 ccagccttca gcatcctata tatctttttt attaaattct catagtctgc tacataatga
     7621 aacgcaagcg aacttagtat tacatcaaag ctctcctctg ggaaatccac atcttctatg
     7681 gcacagcatt catattcaat ctgtggaaaa tgggtttttc cttttgctac ttcgagcatt
     7741 ttatgagaaa tatcaacacc tactacagag gaagcaccgt tttccatcgc atatatacag
     7801 tgccatccat agccgcatcc taaatcaagc acacgcttac ccttaaaatc aggtagcatc
     7861 tttttcaaag tctcccattc tcccgcacca gccagtcctt tctgcgagcg actcatttga
     7921 ctgtattttt gaaaaaatat attatcatca tatttgtttt ctttcatctg aactctcctc
     7981 gtttaaaaag ttatttatct ccgatacaat ttcattcact tctttataaa gtttctcggt
     8041 catgtcgtag cattcaaaaa gagaaagacc gagtacttca aaaatatgat taactctttc
     8101 ggcatatttt tcaggtttat attcaaaagt ttcaagcagt tttatagctt tcttttcgtt
     8161 aatgcaataa gcattattac atgcaaatag tacttgattt aagcatgaaa taatacgaaa
     8221 aacatggcct gcaatataat atttatcctc tgcccccgca tttgctttta caaacattaa
     8281 agagaattca gcttcaaata taaaaaagtt catcaagctt ttctttagag cagtagggta
     8341 aatttctgcc tgctttttta attcgcataa gctttcgttc ttagcatata gtattttact
     8401 aatcgctaat tctccacgat acatagcact tatataacca tggggatgcc cggtctgata
     8461 attggcagta acaattcctt gctccgtatc tttgattatt tgttccaccc gttttatatc
     8521 acgtaatatc aagtcaacat gacacccgtt tataactaac catccaccgc cattaaccca
     8581 atcaccccac gctccgggag gtacaacaag gttgtttcta ttctcatcat ccaactctgt
     8641 agcaatttga ttaatcgctg tcaggtcaaa tgagtctgaa ttgtaataga ttccgatatc
     8701 tatatcagaa ttttctgtat gggtgcctct tgcacgtgag ccccctaata cgatgccttc
     8761 tataaaaggc aaagatgata atttctctgt tactatttga ataacattac ctaccatata
     8821 aacatcctcc tttattcgtt agatattttt atttcttctc catctggtaa aataaatgcc
     8881 tgcacaaact tgacacctag cacatcagca atggccttca actcatccaa ggttactgtt
     8941 tctcgttgta attttttatt gaaattctgt ggagtctggc caatacgtct agcaagttcg
     9001 gaaacactta tattcatttg ctcacacagt tctttaatca tttctgccgt agtcatacag
     9061 cacctccaag tttacgctaa gaatattata aaccatttgg ttgataaaca caatagttat
     9121 taaaatatta ttataatcag caagcaaaag aaaacccaca actctactgt agagtcatgg
     9181 gctatttact tctggttttc atatttccat ttcgatgcca gatttgaagg ctatgacgaa
     9241 gtggccttca tagactgtaa cgctctggat tatcttcctt acaagcttat catcatattc
     9301 taaggtgcgg aatttgttct tgcggataaa ttcaatcagc tcattgattc gctcgttctc
     9361 gccactgagg gaggcatctt ctactaaaag gatctgtcgc ttgtctctca gctcatcaat
     9421 ctcatctgct aagtggtcat agtcttgacc cttgttcgcc agcttgatta gttctttttg
     9481 tttttcctct agtgagttgt taatctctga aatttggtat tctgtggttt cgccaatcac
     9541 agcatgaata ttctcttcca gtgtctttat catattgttc ccacctgcaa gtagcttatt
     9601 aatcgcagtc attactgcac cataaagttc atcttctttt acggttcggt tcttacaaac
     9661 ttcaggacct tgctcgattc tagtcacgca tcgccagaca aattcttttc ttccgtggat
     9721 attccaatag acccgtctat aaatatcgcc acaatctcca cagaaggtga tggcacttaa
     9781 agcgtacttg ctactgtaaa ttcttttgtt cttgccttct cctgtgtaga tattgcttct
     9841 ccgatgaatt tcttcttgcg cctgcaaaaa taactcttta ggaataatcg cttcatggct
     9901 attttcaaca taatattggg gaacatgccc ttcatttttc actcgtttct tggtcagaaa
     9961 atctactgtg acagtctttt gcagaagggc atctccgatg tatttttcgt tttgaagtat
    10021 cttcttaact gattctggtc gccatcttgg ttttcccgct gctgttaaaa taccatcctt
    10081 ttcaagggct cgaccaatgc ccactagact ctgaccctca aggtattctc ggtagatgcg
    10141 tttgataatc tcagcttctt cggggacaat agttaaattt ccatcttcat ctttcgtgta
    10201 gcccataaat cgcttatggt tgacctgcac ctttccttgt tggtatcggt actgtagccc
    10261 aagcttaacg ttttgtgaaa ggctctggct ttcctgctgt gccaaagatg ccataatagt
    10321 cagcaaaacc tcacctttgg catccgtggt gttgatgttc tctttctcaa aataaactga
    10381 tatgttctta tccttgagct gtctaatgta ttgaaggcag tctagggtgt tacgcgcaaa
    10441 tcggctgatg gattttgtaa taaccatgtc gatgttaccg tccatacact ctgcaatcat
    10501 gcgattaaat tcgtcacgct ttttagtgtt cgtaccagag ataccatcat ctgcaaagat
    10561 gccagcaaac tcccattcat tatttttctt tataaactct gtatagtgtg agacctgaac
    10621 ctcatagcta gaattttgtt cttctgtttc tgtagaaaca cggcaatagg cagcaacacg
    10681 aagtttcttt atcttttctt ttgcggctgt acttcctact cttttacgag caggaataac
    10741 cattatattt ttctctgtca ctttattcct cgctttctat cagactgtat aagtattctg
    10801 ctcgttcaaa aggatccact ggcatcttat tatctgcctt tttcattttg aatcgttctt
    10861 tgggaggggg agaggtgaaa gcggcaagct ctaccactcg tcctaaatcc tttgcacgtt
    10921 tatctctaac ttcttcagct ttatcaaata tctctttatc aataattgct ggatacgtat
    10981 catttccaag gtagttgacg tttttcaaaa tgcgccccat cacagaatgt gtcttttcaa
    11041 tacctgcctg ttcgccagcc actgtaaggg atagtcctga tatgtatttc tcaaagaata
    11101 cctttacttg acctgctgcc ttttcatcga cagtaaccac tccatcttga attttgtatc
    11161 catatggaat atatgccatt tatctcacca ccttttcttt cagggaaaga ccgcatttca
    11221 aattgaatgt cagctcatcc ctggaattta caatgatgtt cactacaaat tcttcaaata
    11281 gttcttccgt gtagtcacca ttaaaattat ctgctgacac ataattaatg aggtccttta
    11341 tctcgtttgc tcgcaaaacc ccactcgcag agttcgttac caggtttgtt ttttcggtag
    11401 taagattttt aatctcacta tctaaaacat ttcgttcctg attaaaaaga gcaggttcta
    11461 ggaaaccttt ggacatcagt gcaataaggg tattgcgttc ttccaatagt tgctccatta
    11521 gcttatcaat agcgtccatt ctttcacagt cgctttcttc atcgatttgg ctaactgact
    11581 tgaaaagtgg ttctaggatt agcttatgac tgaaagcaag cttattcatc atggttgtca
    11641 atgtggcttt gatttcccca tctcgcaaaa acaacatgga gcaactttct ttatcttcga
    11701 tactatctca acatgtgtcg tggataaggt aagtatgtaa aatggtatgt ggaacatagt
    11761 gatttgctgt aaatgatttg ctaactactt tagttcttta tacaaagtat aaatcggata
    11821 ttttattatt ttttctttta tatcttctga gattaggtca aagtatttgt ccgctaaatc
    11881 agaaaggggt tcttttttca tcacctaatg ggtgatgaag cagaatactt taaagactat
    11941 taaatcaatg ttaggaaaga ctattagtaa aactatgaat tattcaggaa aaaaggttct
    12001 tttttaaagt tttctattgc ttcctggcgt tgataaatga gatataaagt cggtgtttgt
    12061 acacgtccta atgaaaatgt accttgtacg cctttttgtt gtaaattcaa ggtatataaa
    12121 gggcttgcgt tcatgccgat caaccaatcg gcaatttggc gtgtttgcgc ttcttgataa
    12181 aagggatagt aattcattcc tggttgcaaa ttttgaaaac cgcttcggat cacatctttt
    12241 tctaagctat tgatccataa tcttttatac gttttatctt tagaaaaggc atttgcttta
    12301 tggataatcg accaggcgat attttcacct tctctgtcgc tatctgttgc gacaataatt
    12361 gtatttgcct ttttgagaag ttcagcaaca attttaaact gctttccctt atcttttgca
    12421 acttcaaaat cgtatcgatc aggaaaaatc ggcaaagatt caagtttcca attttgccac
    12481 ttttcgtcat aatgacctgg ttctgctaat tccactaaat gcccaaaacc aaaggtgata
    12541 aacgtttcat ctgtaaatag tgggtctttg atctcaaaat aaccgtcttt tttggtgctt
    12601 tgttttaaag cacttgcgta ggctaatgcc tggcttggtt tttcagctaa aataaccgta
    12661 ctcattaact atccctcttt tcattgtttt ttctttgatc gactgtcacg ttatatcttg
    12721 ctcgatacct tctaaacgtt cggcgattga ttccagtttg ttcttcaact tctttatcgg
    12781 ataaaccatt caaaaacaaa tcgaaagcat gctttaaacg tggatcattt tctttaaatt
    12841 tatgctgacg acctttgaat ttgccttttt tcttagcaat ttcgattcct tgtgcctgac
    12901 gttccttaat tttttttcgt tctgattctg cttgatactt gtacaattca atgacaaggc
    12961 tattaatcaa acgccttaaa ttttcatctt caataccatt cattgagggt aaatttaaga
    13021 cttccagggt tgccccctta atttgaattt gattcatcaa ttctgttaat tctttattat
    13081 ttcgtcctaa tcgatctaat tcagtaacaa taacaatatc cccttcacga atatagttaa
    13141 gcatagcttg taattgtggg cgttcgaccg attgaccgct taatttgtct gaaaagacct
    13201 tagaaacgcc ctgtaacgct tgtaattgcc gatctaagtt ctgttctttg ctactgacac
    13261 gtgcataacc aattttagcc attttcaacc aacctctaaa attctctcgg ttgcaataac
    13321 caatcagcaa tatctacttt ttcaatttca aattgcttat cagaaattgt cttttcgtaa
    13381 gcgataaaat cttgcgcata ttgttgctca ttaaaaatag ccaccacttc gtcattttct
    13441 aaaactcgat aaataaattt tttcatttta ctcctcctat tatgcccaac ttaaatgacc
    13501 tattcaccaa gtcaattata ctgctaaaat catattagga caaataggta tactctaatg
    13561 acctataaaa gatagaaaat taaaaaatca agtgttcgct tcgctctcac tgcccctcga
    13621 cgttttagta gcctttccct cacttcgttc agtccaagcc aactaaaagt tttcgggcta
    13681 ctctctcctt ctccccctaa taattaatta aaatcttact ctgtatattt ctgctaatca
    13741 ttcgctaaac agcaaagaaa aaacaaacac gtatcataga tataaatgta atggcatagt
    13801 gcgggtttta ttttcagcct gtatcatagc taaacaaatc gagttgtgtg tccgttttag
    13861 ggcgttctgc tagcttgttt aaagtctctt gaatgaatgt atgctctaag tcaaaagaat
    13921 ttgtcagcgc ctttatatag ctttcttttt cttctttttt tactttaatg atcgatagca
    13981 acaatgattt aacactagca agttgaatgc caccatttct tcctggttta atcttaaaga
    14041 aaatttcctg attcgccttc agtaccttca gcaatttatc taatgtccgt tcaggaatgc
    14101 ctagcacttc tctaatctct tttttggtcg tcactaaata aggcttgtat acatcgcttt
    14161 tttcgctaat ataagccatt aaatcttctt tccattctga caaatgaaca cgttgacgtt
    14221 cgcttctttt tttcttgaat ttaaaccacc cttgacggac aaataaatct ttactggtta
    14281 aatcacttga tacccaagct ttgcaaagaa tggtaatgta ttccctatta gccccttgat
    14341 agttttctga ataggcactt ctaacaattt tgattacttc tttttcttct aagggttgat
    14401 ctaatcgatt attaaactca aacatattat attcgcacgt ttcgattgaa tagcctgaac
    14461 taaagtaggc taaagagagg gtaaacatga cgttattacg ccctattaaa cccttttctc
    14521 ctgaaaattt cgtttcgtgc aataagagat taaaccaggg ttcatctact tgttttttgc
    14581 cttctgtacc gcttaaaacc gttagacttg aacgagtaaa gcccttatta tctgtttgtt
    14641 tgaaagacca atcttgccat tctttgaaag aataacggta attaggatca aaaaattcta
    14701 cattgtccgt tcttggtatg cgagcaatac caaaatgatt acacgttaga tcaactggca
    14761 aagactttcc aaaatattct cggatatttt gcgaaattat tttggctgct ttgacagatt
    14821 taaattctga ttttgaagtc acatagactg gcgtttctaa aacaaaatat gcttgataac
    14881 ctttatcaga tttgataatc atagtaggca taaaacctaa atcaatagcg gttgttaaaa
    14941 tatcgcttgc tgaaatagtt tcttttgccg tgtgaatatc aaaatcaata aagaaggtat
    15001 tgatttgtct taaattgttt tcagaatgtc ctttcgtgta tgaacggttt tcgtctgcat
    15061 acgttccata acgataaacg ttgggtgtcc aatgtgtaaa tgtatcttga ttttcttgaa
    15121 tcgcttcctc ggaagtcaga acaacaccac gaccgccaat catgcttgat tttgagcgat
    15181 acgcaaaaat agcccctttg cttttacctg gcttggtagt gattgagcga attttactat
    15241 ttttaaattt gtactttaac aagccgtcat gaagcacagt ttctacaaca aaagggatat
    15301 tcattcagct gttctccttt cctataaatc ctataaaata ggttgtttaa ttaacttggt
    15361 ttgctttttc attcaactgt ttcaatattg catgttttga aaaagatttt tttcctttat
    15421 aagtcaattt ttttccacta atcgaataaa ttattttgtt attttctatt aacttatata
    15481 tataatcttc cccctccgaa gaaaaatact tatctgattt tgtttctaag tagatatttc
    15541 tcttttctaa ctctttctta aacgtttcta gtgtatagat atttgctaat tttcttatct
    15601 ccaataaact attttttata taagttttac attcatcatg attcatacaa actccacctt
    15661 ctataaatga atacaaaaaa agcaatcaaa cgatttccga ttgattgctt aacaattctt
    15721 aaattcagta gcttagatac ttgaaaactc tctgatttcc ctatataatg atagtacggt
    15781 tatataccgt cttcaaacaa agttaattaa ataacttctt acgagggaag agttcatctg
    15841 actaactgat aagcgttggt ttggcaatct tatcgggcta tgcatttata aaatgtcgtc
    15901 aaacatttta taaatgtgtc atggctcttt tttcgtttct attcagttcg ttgtttcgtt
    15961 atatctagta taccgctttt aaaaaaaata agcaacgatt tcgtgcatta ttcacacgaa
    16021 gtcattgctt ttttcttctt ccatttctaa atccaatgtt acttgttctg attctgtttc
    16081 tggttctggt tctgttggct catttgggat taaatccact actagcgttg agttagttaa
    16141 ctttgcaatt tgttctagtg tttttatggt tggatctgat tttcctgatt ctattcgtga
    16201 ataatttgat ctactcattt ctaattcttg ggagaatttt tcttttgatt tggtacctct
    16261 catctttttt aagctttctc taaatgctag ttccaagttt ctcccccctc tttttgtgta
    16321 ataaaattca cgctttattt aaattatagc ttttaatatc ttaataaaca agtgattaat
    16381 cacttgtgat ttgtgattat tccaaaagta ggtaatttca ccaacctttt tcaaaagttt
    16441 ttatcgacga ttattggact ggatcgtgtt tcctaaagcg attctaagcg catgtttaat
    16501 tttaaagtgg gtaattctct ttaaaacgtc ctacaaagat tcaaaaaaag catggctgct
    16561 agtgggggta ccgtcaggaa aatgcggatt tacaacgtta aggaaattta ttaaagagac
    16621 caattcctca gcactaaaat tgataattgg gctttctttg ttactatagt gttaggtaag
    16681 ctttttactt tatcaccggt tttcccctca cttcacacct acgggcgagt ttccccacat
    16741 cccgttttcc atctaatcca attcattcaa tatctttatg tgtatgaaag atcagattgc
    16801 ttgatttaga catcttacac gtaatttatt cttaaatggg tacggtaaac gagcaataat
    16861 acgagcaaaa gtatacctac aaaaataata actctgtcta attttttttg attcatatgc
    16921 ttattgctta aaccatataa tttcagtcca agaaagcctc caacagtatt tgtaattaca
    16981 tctgttatgt ctgtcgctcc aatagcgaag ataaattgaa ttatttcaaa agtaagactt
    17041 aaaaccagta caaaagcaaa cttaggtaaa aatccgattt ctttaaaatt gacattcaaa
    17101 agcaagccaa atggaataaa gattataaca ttatctatca tctctctgaa attcccagta
    17161 gcagtaaatg gagtcaagtt aagacttctt tgatgataat taaatactga taaaatattg
    17221 tattgtaatt tgaataacac taaccagatt agtgtcacta aatataaagc tagcaatcct
    17281 ctagataata tttttcccaa tataatttcc tctaattctt ttgtttaagt tgtatcatta
    17341 gtatagcaat gtcctagtta acgtgggttt taaattaaaa agctaacgtt accccaaaag
    17401 tgctagtaca ataatgctgt tcattaattt cgttggtttc attccgccat ccttacctcc
    17461 ttgaattagt atgtgttgat ccgggaaaca ctgtcacaat tacaccgtct atattgtttc
    17521 ctgatatctc ataacgaaga ttagtcggca catgaatggt ggtattttta gtaacaggat
    17581 aataaaagat ctcatatttt tccccattta cactaacaga aatggttttt tcttctttta
    17641 ggtaatccat atattcctcg agaacgaaat tcttttcttt cataatcgca ctatgtggta
    17701 aaccaacata gcgaatatgc catggttcat attgaattcc tgttaactct gttttgtcct
    17761 ctggataacg taaaatgaac ccgtatttcc aagcattttc ttctatccac tttccttcag
    17821 gggctcgttc cattttcgtc aagcttgatc ctacatctag tgataaacct gaattatgct
    17881 cactataacc tgctggtaag gcatactcag cccccatttc ttggtaaagc acactttgct
    17941 catcaaagtc tcgatagcca ctattaataa taaaatgact aacgccaccc tttacagcat
    18001 cattgaccat ctctgaaaat ttttgtgcta tttcttttga catataaata ttactatcaa
    18061 gcaacccgta tccatttatt aattcgtcat gtttagataa attcacgata tctgacttca
    18121 cactttcttg gcgaacagga tatttactat tgattaatag cagatttcct tgataaacct
    18181 gttcttctgt aattgttttc tcttgggtat tttcagaagt cccactattt tctaaatgtt
    18241 ctttgggatt ttgatcataa ttttgaaatt cgactttttc ctgagaaaac agtgcttcat
    18301 taacgtagtc ataacctaag tatattaaga ataacaataa caataaaaaa aacaacttct
    18361 tcattttcag tctcctcctt aactatatag ttggaagtat gaaatatttc tagaatcttc
    18421 cccttaaacg gcacaatctc gtttgagatt gtgccgtttt gccccgtaaa ttacagtcaa
    18481 ttatttcgcc ctactggatt tactattatc accaatgtag atgaattttc ataagtgata
    18541 cccatgctga atttaacgag gctgaactgc ctgttgacaa tg
//
LOCUS       DAZKOR010000056        18504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000056 DAZKOR010000000
VERSION     DAZKOR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.056"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8ZM_001817"
     CDS             99..746
                     /locus_tag="ACG8ZM_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0492769.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8ZM_001818"
     CDS             1139..3784
                     /locus_tag="ACG8ZM_001818"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0492770.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8ZM_001819"
     CDS             4094..5407
                     /locus_tag="ACG8ZM_001819"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0492771.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8ZM_001820"
     CDS             5397..6050
                     /locus_tag="ACG8ZM_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0492772.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG8ZM_001821"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG8ZM_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0492773.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG8ZM_001822"
     CDS             6947..7147
                     /locus_tag="ACG8ZM_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0492774.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG8ZM_001823"
     CDS             complement(7401..9080)
                     /locus_tag="ACG8ZM_001823"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0492775.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG8ZM_001824"
     CDS             complement(9082..9294)
                     /locus_tag="ACG8ZM_001824"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0492776.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8ZM_001825"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8ZM_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0492777.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8ZM_001826"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8ZM_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0492778.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACG8ZM_001827"
     CDS             13269..13466
                     /locus_tag="ACG8ZM_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492779.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACG8ZM_001828"
     CDS             13619..14104
                     /locus_tag="ACG8ZM_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0492780.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8ZM_001829"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8ZM_001829"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0492781.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACG8ZM_001830"
     CDS             14981..15508
                     /locus_tag="ACG8ZM_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492782.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACG8ZM_001831"
     CDS             15599..16336
                     /locus_tag="ACG8ZM_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0492783.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8ZM_001832"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8ZM_001832"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0492784.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8ZM_001833"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8ZM_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0492785.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3200 c         4022 g         5298 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAZKOR010000057        18216 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000057 DAZKOR010000000
VERSION     DAZKOR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.057"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..859)
                     /locus_tag="ACG8ZM_001834"
     CDS             complement(35..859)
                     /locus_tag="ACG8ZM_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0492786.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(832..1512)
                     /locus_tag="ACG8ZM_001835"
     CDS             complement(832..1512)
                     /locus_tag="ACG8ZM_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492787.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1535..2473)
                     /locus_tag="ACG8ZM_001836"
     CDS             complement(1535..2473)
                     /locus_tag="ACG8ZM_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0492788.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2718..3569)
                     /gene="ispE"
                     /locus_tag="ACG8ZM_001837"
     CDS             complement(2718..3569)
                     /gene="ispE"
                     /locus_tag="ACG8ZM_001837"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0492789.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3723..6296)
                     /gene="mprF"
                     /locus_tag="ACG8ZM_001838"
     CDS             complement(3723..6296)
                     /gene="mprF"
                     /locus_tag="ACG8ZM_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0492790.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6437..7243
                     /gene="proC"
                     /locus_tag="ACG8ZM_001839"
     CDS             6437..7243
                     /gene="proC"
                     /locus_tag="ACG8ZM_001839"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0492791.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7322..8878)
                     /gene="rny"
                     /locus_tag="ACG8ZM_001840"
     CDS             complement(7322..8878)
                     /gene="rny"
                     /locus_tag="ACG8ZM_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0492792.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9289..10338)
                     /gene="recA"
                     /locus_tag="ACG8ZM_001841"
     CDS             complement(9289..10338)
                     /gene="recA"
                     /locus_tag="ACG8ZM_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0492793.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10447..11688)
                     /locus_tag="ACG8ZM_001842"
     CDS             complement(10447..11688)
                     /locus_tag="ACG8ZM_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0492794.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11928..12506)
                     /gene="pgsA"
                     /locus_tag="ACG8ZM_001843"
     CDS             complement(11928..12506)
                     /gene="pgsA"
                     /locus_tag="ACG8ZM_001843"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0492795.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12715..13638)
                     /locus_tag="ACG8ZM_001844"
     CDS             complement(12715..13638)
                     /locus_tag="ACG8ZM_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0492796.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14376..15662)
                     /locus_tag="ACG8ZM_001845"
     CDS             complement(14376..15662)
                     /locus_tag="ACG8ZM_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0492797.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15655..16923)
                     /locus_tag="ACG8ZM_001846"
     CDS             complement(15655..16923)
                     /locus_tag="ACG8ZM_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0492798.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17175..17636
                     /gene="mscL"
                     /locus_tag="ACG8ZM_001847"
     CDS             17175..17636
                     /gene="mscL"
                     /locus_tag="ACG8ZM_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0492799.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17695..18141)
                     /locus_tag="ACG8ZM_001848"
     CDS             complement(17695..18141)
                     /locus_tag="ACG8ZM_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0492800.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         5193 a         3982 c         3184 g         5857 t
ORIGIN      
        1 ttttcgtttt tataaggctt cttttcttta taatttagtt attttttctt ctgaaggcaa
       61 ccaatattcg tttgatgata tttacaatca ggaacaaagc aataaagact aatgtgattg
      121 ttgctcctgg aggtgtatct agataaaacg agctagttag tcctgacagc atccctagaa
      181 atcctacaca aacactgata gcaatcacga cattgaagct tttgcctaat cgcataccga
      241 ttgctgcggg aagaaccatg attgcggata tcaaaagtgc accggcaatc gggatcatca
      301 cagcaatagc cacaccagta atcacattga acatcatcga catcaaatga acgggtagcc
      361 catccacgtg ggcggtatct tcatcaaaag tcagaacata catcggccgt ttgaataaaa
      421 agaacaatat accgatggca acgaataaga ttcctaaaaa gatgacttgc tcccatgtaa
      481 tggtcacgat cgaaccaaac agataggatt ggatacttgt tgctgaattc cctccgctca
      541 aattcatcaa tactaacgct agcgccaaac caccagccat taaaatggca atggagattt
      601 ctgaataggt tcggtagatc gttcgcagat actccaatat cacagctgct aatacaacga
      661 ctagcatggt cgtaagtgta ggattaaggt tcaaaaagaa tcctaacgct acccctgcta
      721 aagagacatg agaaagcgta tcagccatca gcgattgtct tctgatcact aaaaacacac
      781 ctagcatcgg ggcgattccg gcaatgaaga tagcggcaag aaacgctctt ctcatgaatt
      841 catatgaaag caacgccatt gagaatcctc ctttctcacc agtcggatct gtctgtccgc
      901 atactgtttg atatcttcat gatcatgggt gatcattaga actgatttcc catggacatg
      961 cgcactatgt cggagaagac gataaaattc atttctggat tcttcatcca tacctgttgt
     1021 tggttcatca agaacaaata agtcagggtc agtagcaaaa atacgcgcta aactgattcg
     1081 ctgcttttgt ccgccagaca attcacctat tcgccgattt cgcatatccc acatccctac
     1141 agcttctaaa gcacgctgta catgttgatg atctttttca gagagacgct tgaaccagcg
     1201 gcctcttgga tatcttcctg aacggactaa ttcgatcacc gtactaggaa aacctgcatt
     1261 gaaagatgca acttgttgag gaatataacc gatacttaat ttttttcctt cttgattttt
     1321 ctcagcaatt tttatcattc cttttgaagg tttcagtaat cctaacgatg ctttcaccaa
     1381 ggttgacttg gctgctccat tttctccagt taaaatgaca aattcacccg catctacatg
     1441 ataagagaca tcttctagta caggttcatc atcatagtaa aaggtcaaat cagatatttc
     1501 gatataacgc accttacaac ctccattcta aaacttaatt gatactttct tttaaagctt
     1561 tcagattttt ttccatgaca gaaatataat tttcgcctgc tttgatttgt tcattagtca
     1621 agctttctaa aggattcaac acttctagct tcactcctgt ttcattcgac aatgtctcag
     1681 ctaccttcga agaggcattt tcttcaaaat aaatcacttt gatgttgttt ttcttcacaa
     1741 attcttttag ctcagctaag cgacttggtg tgggttcctg atctggtgaa acgcccgaaa
     1801 tcgctacttg ttttaagtcg tattgattcg ctaaataagc aaaagcagca tgctgagtga
     1861 caaatgtgcg gtttgttgca tcttttagct catcggagta tttttgatcc agtgcttcaa
     1921 gctttttgat ataagcatcg gcattcttcg taaaagtttc ctttttatct ggatattttt
     1981 cgcctaattc ttcagcaatc gttctgactt cttttatagc taataccgga tcgagccaaa
     2041 catgtggatc gtattcatgt gaatgaccat gcccttcttc tgattcttca tgttcgtctg
     2101 cttcttgcga ttctaaacgg tcgattcctt tagctgcttc gatcatcaag gtctgtttgc
     2161 tatccactga atcctttaac gaatccacaa aaaattccat gtctgaacta ttatacacga
     2221 aaacatctgc atcggaaatt tctgctcttt cttttgcact tggttcgaaa tcatgcggct
     2281 ccgttccagc agggatcaaa agtttcacgt ttccttcatc ccctacgatc tcttttgtaa
     2341 aatcatacat cggataaaaa gtcgtaacga tcgtcaaatc ctcttttttg tctgcttcct
     2401 tgttcgtatt tccacatgct gcaaacagta aagcacctat cattaccgta gctgtcaaaa
     2461 gatatttttt catttatatg ctcctttcaa tgaacaaatc ggaattttac gatttattaa
     2521 cgtgaactaa tgtatcaaaa taaaaaaaca cagtcaatag aaatccatca atttctgatt
     2581 taagcatgta tattcttgat taaatcgtaa aatggatgag ttgctgctca tatctagcca
     2641 tttcactaca ataaaaaact gaagaatacc attttaggat tcctcagttt ttcatcacct
     2701 attttacttc ttatatttta ttgcagggtt cgtactaaat aaacttcctc acaaaaacct
     2761 tttaacccat tatatactct ctgcgctctt gaataatgtt gacacaaggc aaaaacggtt
     2821 ggtccgcttc cactcatcaa tgcagcatct gctccgtact tgatcatacg ctctttgatt
     2881 tgatggatga ctggatgccg ctttcccgtt atatcttcta aactgtttcc catttttttg
     2941 atcatctgct gataatcttg ctctcggatg gcttcagcaa gtccgtggat gtcttgatgt
     3001 tggatggctt gcgtatctac ttctctaaac actgtacgtg tagagacact cattcttggt
     3061 ttgaccaaca caacccagca ctgtggcata gaaggtaata cttggatttc ttcccctttg
     3121 ccaccgacaa atgccgtact tccgtagaca cagtaaggaa catctgtacc gacttccaaa
     3181 ccgatttctg ccagttcttc taacgaacag cccagattcc ataattggtt gatccctctt
     3241 agagcagctg cacagtctgt acttcctccg cctaatccag ctgctactgg aatattcttc
     3301 ttaacaaaga tcttgacacc ggttttgata ttgtatttat tttttagcaa taaagcagct
     3361 tgataaacat tgttccgttc atctaccggt aaaaatgctt tatttgtttc tacaatgatt
     3421 ttgtcttctg ttatttcttc aaaaaataga tgatctgcca agtctacgct cgacatcacc
     3481 atttccaact catgataccc atcttgccgt ctatataata catccagtcc taaatttatc
     3541 ttggctggcg ctttttcaat caattccatc ctctcaccca ttcgaaaagt ctaagtcgca
     3601 agtattctat catagggaaa aataaaagta tagcttatgt ctgcctagtg gttgattttg
     3661 tcatcgtttc aacagctttc acagaatttt ctcttcataa tccaaacaag ttctagtgat
     3721 tcttataatt ctgtgagcac ttcttctgcc aaactcggaa catttccttt ttcttttcct
     3781 atcagactga gtagctgcat ggtagcaaat ccgaaattac ttcctttcct ataagcaaaa
     3841 taacgtcctt cccaatgatc gacatatttt aatttctttt cataagcaga ctggaaggaa
     3901 tagaaaaaat taccataatt atatacgata tgcatgactt tttcttgaaa gaaagaaaaa
     3961 tctgtttctc caacattagc taatggagct aagcctagat tggcttccga gtagcccatt
     4021 ttctcgtata caataaacag attggctaac agatagtcag ctaattcctc agattcttta
     4081 ggtaagaccc gcaacaaatc atatcccgcc tgcttcttcg tcatcggctg agcggtgatg
     4141 aagccaacga ctttttgatc tttacggaca ataccgacac cgctagataa tacatatgat
     4201 ttatcaaatc gtcccacaga gaagtatttt tctcgtgcac cttgaagcca tttttgtgaa
     4261 acttcattta cagcttgatg taattcttcc ggcagttgat cgtaccaagt aaaatggtat
     4321 ccttctttca tcaaacgttg atattcaagt tgctgactca gccgagacaa gtcttctgtt
     4381 gctagatcaa caatccctgc ttcacctact ttcgtgaagt gaaaacccaa ttcatgaagc
     4441 atcactacgt agctttctga tatcttataa aacgcaagct gatagcctaa acaatctgct
     4501 tgtttcataa actgattcgt tgctgcccgc atcattttag gattgccaac tggatctcct
     4561 aaaacaaagc atttgtttcc tttgatctga taaccaaaaa cgacttgatc ttggtcgtct
     4621 tcttggtaat aaaaataact gtaacctgac aagcgaagct gatggcttgt agctgtgcct
     4681 ccgtatgttt ccaataaacg gtcaaaacgg tctttttcgt actcactgcc aagcttcgta
     4741 gtatcttcag ctaaatattg ataaagcgcc actaaagcta gcaatgagat gcccaaacca
     4801 ataagccctg agaaccacac atcatctgaa ggaaacaaaa taaaatgatt tcctgttatt
     4861 cgaccgacta aaaagcgtcc gctgtgatag cctgcaattg cgtaaacgat aaacaaaaag
     4921 ccgaacaagc tagcatcaaa gattatagct ccccaagaat aaacgaattt tgttcgataa
     4981 aattcttttc tggataacca cacagctaac agaatcaaga catataccaa tatcaactgc
     5041 caagataacg tacgtgtgat tgtatttaaa ataccaaaag atagtaaagc aatcgtaggc
     5101 agaaatgcct ttttgacttt tactgaaagt gctctagcaa ggccgagtaa taaaaagcca
     5161 accaatagat tcactgtctg atctaaaaaa ttgaatgaga acggcaataa tacttgaaat
     5221 agccgactga ttgcagacag attagtgatt gttgatagaa gtaccatcat gatccccgca
     5281 aaatagacag cacttacgag cacgatatgc gcaacctttt ggaaaaacag tcgcggaatg
     5341 ttatctaaaa atcgattgat tttgactcct gtatgtgtga tgaacaatag aagtcccgtc
     5401 ataaaaggta gaacataata aaacaaccga taatataata gccaaactac tgctacatct
     5461 tgccgaacag gcaattgact cattcctaag atcatcaaaa cgtcaaaagt ccccattcca
     5521 cctgggacca tcgtcagcat tcctatcaat gtagcaatca tgaacatagg atagatcgaa
     5581 agcatcgaaa tatctattct catcaacttg ccgatgatca aaaataccgc aagggctccc
     5641 gtccattgtc caaaggatgc cagtagcaga ccaagcaagc cttttggata aaactcgaag
     5701 aacaatgtct ttctctttag ataagcaaaa aagaaaatga ctggtgctaa aaaactaccg
     5761 ccgaaaagcc agatccaata gcttcgaaaa atactttctg actgaataaa aaagacatca
     5821 ataaatgtaa gaaacgacca tacagacaaa ccagtcagca taaatagtgc gacttttgat
     5881 acagtggcta aaaccatttt tctatttgtc tgctttccat aaaaactagc ccgcagactt
     5941 gctcctacca caccaccaaa accggcgaga ttgttgatcg tgtttgttac ccacgcagca
     6001 agaagaaact cttttctttc catttttggt tttccttgtt tttctaagac tctgatcgtc
     6061 acccaatcat acaacaacat cggagtgaca cccagcagtc cagccagaat catgagcagg
     6121 atcgtcatgc ggctttgttg tcccatcctt tgccaaatat cttgccaact catgccatta
     6181 gcaatatttg cgacttgatt tgccacaaag actaacacgg agccgaaaaa aatcagtttt
     6241 aaaatcagac tatgttcctt tatccattgc agcatcaagt atcgtttatt tttcacattt
     6301 tcccattcct aacttgtata cttttgtgtc ataccccaat gttatcttat ttattttaat
     6361 ttttccactt tagacctgtt ctttgcttgc gatgatagaa aaagaaagat atcctaatgc
     6421 taaaggagag gataacatga aagtaggatt tatcggcaca gggaatatgg cacaagctat
     6481 tattgatggt ttattaaaaa aaggaacagt ctcgaaagaa gaggtgttcg tttccagtgg
     6541 acattttgaa aatgcacaaa aatttgctgc tgaaactggc gtccatgcat gcaaaagtaa
     6601 tcaggaagtt gcatcacaat cagatatcgt tcttttagcc gtgaaaccaa aaatcattga
     6661 aaaagtattg gaagaactaa gagaatatag ccagaaagtc ttattcattt ctattgcttc
     6721 aggaaaatca ttaacagatt tagaaacagc gcttgaagat cctcaggcct ccatcgtacg
     6781 tgtgatgccg aatgtcaatg tcagcgtagg agcaggcgtt tctgcgattt gcaaaaatga
     6841 agctgccacg gaggaacagc ttcagcaagc tacgatgatt tttgaagcaa tcggcagcgt
     6901 ctatccttta gcagaatcag atttcagtac attcattggc cttgctggca gctcacccgc
     6961 attcgtttac atgctgatcg atgcgatggg acggactggt gtactgaatg gtttgtccaa
     7021 acagacagcc actgagattg ctgcaaaagc aattatgggt tcttgtcaaa aattcctttc
     7081 aagtgataaa aatccatggg aacttgtaga ccaagtttct tctcctggcg gtacaactgt
     7141 tgcaggtgta gttgccttag aagaagcagg attcatccca gcaatcatca aaggaatcaa
     7201 tacgacgatc gagaaagatc ttgagatgtt gaataaaaaa taatgatagc aatgtttttt
     7261 cgtgccttac aaaaaaagaa gagctgtggc ttttgccgcg gctcttcttt tgtatttttt
     7321 attatttagc ataatcagtt gcacgagttt cccgaataac gattactttg atgtgtccag
     7381 gataatccag ttcttcttcg attcgtttac gaatatcacg tactaaacga acagcgtcta
     7441 aatccgagat ttcttctggc ttgaccatta cccgaacttc gcgtccagct tgaactgcgt
     7501 aacttgattc tacaccggcg aagctgttcg aaatattttc cagacttctt aaacgacgga
     7561 tgtagttttc taatgattca cttcgcgcac caggtcttgc tgctgagagc gcatctgctg
     7621 cagctacaag gacagagata acagaagtag cttccacatc tccatgatga gaagcgatcg
     7681 cgttgattac tactgggttt tctttgtact tggcagctaa ttccgcaccg atctcaacgt
     7741 gagatccttc aatctcatgg tcaagtgctt tcccaatatc atggagcaac ccggcacgtt
     7801 ttgctaattg gatatcttcg ccaagctctg ctgctaagat accagctaat tttgcaacct
     7861 cgaccgaatg cttcaagacg ttttgtccat aactagttct gaatcgcaga cgtccgatga
     7921 tcttgattag atctggatga agtgtatgag caccgacttc aaacgctgcc tcttccccgt
     7981 attcgcgaat acgttcatcc atttctttac gagatttctc gaccatttct tcgatacgcg
     8041 ctggatggat tcgtccatct tgtatcagtt tttccaaggt catccgtgcg atttctcttc
     8101 ggattggatc gaatccggaa agaacaactg cttccggtgt atcatcgatg attaaatcga
     8161 taccagtcag cgtctcaagc gttcgaatat tacgaccttc tcgtccaatg atacgacctt
     8221 tcatttcatc attcggaagg gtaacgactg aaacagtcgt ttctgaaact tgatctgctg
     8281 cgcagcgttg aatcgctaat gataataagt ttttcgcttt gcgatctgct tcttctttgg
     8341 cacgctgttc agattctttt accattagag ttaactcatg attcagctct tcttcagtcg
     8401 atttcatgat gacatctcgt gcgtcctctt ttgataaacc agcaattttt tctagttctt
     8461 tttgttgatc ttcaatcatt ttttcaacgt ctttttctcg ctcttcaatt aattgctgtt
     8521 ttgaactaag tgcttcttct ttactttcaa gtgagttctc gcgtttttct aacgagtcat
     8581 ctttacgatt cagcgtttgt tcgcgttgaa tcaaacgatt ctcttgagat tttaattcta
     8641 acttactttc tctcagctca ctttcaactt cagaacggta tttctgattt tcttccttag
     8701 cttctaacaa tgcttctttt tttagagttt ctgcttcttt ttttgcacta tcgatgattc
     8761 cagcagctga tgattgtgca ccagctattt ctttctcgtg acgtgacttt gcgaccataa
     8821 accctaaacc aagaccgaca attaaaccga tgatagcgag gagaatgttg aataccataa
     8881 aatccacctc catactatct ttttcatttt tatcatctat acagcttcta tgataaatta
     8941 ttctaaactc gtcaatatgc atcttcgcat atatgtgtac aaaaaacatg cacaacttta
     9001 ttctaatgtt tgtaccccgt agtgtcaact ttaaataaac aaagaaacgg ctttctactt
     9061 attcattaaa cgatggtttt taaaataaat gacactagtt tcatataggt aaacctttaa
     9121 attgagaaac ttttctatta aattattctt tgtagatgaa cagatatttt tattttatgt
     9181 acccactcgt ataattaaat aactcacgat ttctttattc aataaaaaaa cggcaggtca
     9241 aggagagtac ctactcttca gaccagccgt ttttgtttat cataattttt attcttctac
     9301 taaaggcaat tcttcttgtc ctttttcatc ttctgggaca gcgacttctt caccaatacc
     9361 ataagctgct ctaactagag cagaaacttc tgccatcatt tcaggatgat tagccatata
     9421 gattttagcg ttttcccgtc cttgaccaat ccgatcttct ttataagaat accaagcacc
     9481 gcttttatca acaatgtctt tttctactgc catgtctaga agttctcctt cttgtgagat
     9541 ccctaaacca tacatgacat ctacttcagc gattttgaaa ggaggtgcta ctttgttttt
     9601 tacgactttg atctttgtac ggttaccaac aatatctgta ccttgtttca attgttctgc
     9661 acgacgtact tccaaacgga tcgttgcgta gaatttcaat gcgcgtcctc caggagtgat
     9721 ttctggattc ccaaacatca cgcctacttt ttcacggatt tggttgatga aaatagcgat
     9781 tgtttttgtt ttgttgatcg aaccagagag tttacgcaat gcttgagaca tcaaacgtgc
     9841 ttgtaacccg acatgtgagt cacccatttc gccgtcgatt tctgctcgtg gaactaaagc
     9901 agcaactgaa tcgactacta cgatatctac agccccactt gatactaaag catcagcgat
     9961 ctctagacct tgttctcctg tgtcaggctg tgaaagaagt agttcatcga tattcacacc
    10021 taatttttgt gcatattgcg gatctaacgc atgctcagca tcaatgaaag cggccgttcc
    10081 gccatttttt tgtacttctg caatagcgtg tagtgcaact gttgttttac ctgaactttc
    10141 aggtccatat acttcgatga tacgtccacg ggggtaaccg ccgacaccta aagcgacatc
    10201 taacgcaaga gagccacttg gaattgtaga gatttgttgg tcgatttttt cgcccaattt
    10261 cataatagag cctttaccat agctcttttc aatttttttc agtgcagcat ctaaggctgc
    10321 tttacgatca tctgccaata cgataatcct ccttatattt acaaatcaga gcaacatttc
    10381 attatcctta tcatatcttt tttagtgaaa aaaagcaaga aaaaaccgaa caaatattcg
    10441 catttatcat tttcttaaaa gctgttttct caaaagatca aatccttgca taaccgcatt
    10501 atatctgata gcagcacgat ctctggcaaa gtgatatttt tccgaaacca cgccgtcttt
    10561 agatgaaata gcgatccata ctgttcctgc agattgacct tctaacgtgt ccggtccagc
    10621 cactccggtg aaagctacac tgtaatctgt tcttgctatc tcacgtgctt gaatggccat
    10681 ctgctccaca cattctttgc tgactgttcc atattctgct aatagctccg gatcaatctc
    10741 cagaaaacca gcttttgttt ggagcgagta ggtaacgaat ccgcctggaa atacttcaga
    10801 tacaccagca atgtcgccta atgcggcttg gaaggcgccg gctgtcaaac tttccgctgc
    10861 tgttacagtc tgcttgtttt ctttcagtag ttccactacg acttttgcta gcgaattatc
    10921 atcgccgtat ccataaaaat attcccctac tcgttcttgg attttttctt ctaatgctaa
    10981 aagagcttga ttccctgcct gaacatcatt tgttttcact gtcagtcgta atgtcacttc
    11041 atttggtttg gcatatggtg cgatcgttgg attgatctgt gtctcaatca gatcttttag
    11101 ctcagtgacc agtcgcgatt caccaatccc ataaaagcgc aaaactttag aaattagttt
    11161 ttcttcagaa ggaaaacgtt cctctagtaa aggacgtact tgttccacaa acattggttt
    11221 caactcatta ggcggaccgg gaagaaggat atacgcatgt gtatctgttt gataaaaagt
    11281 ccctaaagca agccctgtac gatttggtag aggaaccccg ccttcgataa tctgggactg
    11341 ttgcagattg tttttggtca tttttcgatg ggttgtttca aaatatgcca ataatttttt
    11401 gtatccttcc gtattttgta tgagagactt accgaggtga gccgcagttg cttcttttgt
    11461 caggtcatct tctgtcggtc ctaatccgcc acataagagt atgagttcac ttcttgtttc
    11521 agctagagca atcaactctt ctaaacgctg ctgattatct cctacaactg tttgataata
    11581 aacttcgata cctagatcag ctagctgttc tgacaagaaa gtggcattcg tattcacgac
    11641 ttgtcctagt aaaagttctg ttccgacagc tattatttct gctttcatta cttcccgctc
    11701 ccttatatag tatacgctgt atttttatat ttttttattt aaaaagaggg caaggagctg
    11761 tgacaaaaat cttgtcacag ctccttgttc caaataaaca gtgttctcct gccaaaacct
    11821 cggtcataag tcacaatata cataaaacat gaaaagctgt tttttgaaat tatcccttgt
    11881 gactcagagt tagacggcag gctcacaacc tctccactaa acgatactta cattgatcct
    11941 ttgaacacac cgctgttttt gatgaagtaa tccacacctg aatagatagt aaagatcaga
    12001 caagcataaa gcatgatttg atctactgga ataccgatca ggctgaatgg gatattattg
    12061 ataaacagca agataatagc aaccatttgt gtagccgtct tgacttttcc cggccaagct
    12121 gcagccatga cttctccatg ttcgacaagt agtaaacgca agcctgtgac ggctaattca
    12181 cgacagacaa tgatcgctac tacccaacca ggggcctttc cttggtcaac caacatgata
    12241 aaagcagtca taactagcat tttatctgcc aacgggtccg caaacttgcc aaaattagtg
    12301 acaaggcctc gcgcacgggc aatttttcca tctaaccagt ctgtgatact agctaaagca
    12361 aaaataactg cacctactag ctgtgtgaca gccagtgtgg tgtctcctac gttaaattgt
    12421 ccccaatcta aaggtaaacc tacaaccaaa atgaaaatcg ggatcataca aattctaagt
    12481 acagttaatt tatttggtaa attcacacta tcgactcctt tcttccctat tctacgtgac
    12541 tatcagcgaa gtttccaccg atattattta cttttccaaa tgaatttatt caaaggagta
    12601 tttttattga aattgatctt tttgaattaa cgaagaagga ccgcgacaca gatttgtcac
    12661 gatccttctt ttttatacct acaagtaaac agatgcgtaa actaatttat acgatcattc
    12721 tgcgtattga agagtaaggt tcacattttt tggactctga ttgttttccc ctggattaac
    12781 tggtacggat tctccattcg ctttgatcgt tgcattgctt gctgcaccaa tcgtgatggt
    12841 cgcatttgtt gctgtttccg gtaacacggt actctgtgtt tcatttgctg ccagtgtccc
    12901 ttgataaaca taggcattgt ctactagcac acctaaccat acacgattga cagcagtaaa
    12961 ttcaagggtt acaggcttgg tagcgtctgt gatatttact gcgatggcac tgccagaatc
    13021 ttctcctgta gctattgcca ttttcttttc ttcacttgat tttgttgttg attctactgt
    13081 agaagactct ttggtctgcg tgctttgctc tgaggaagag gactcctcga ctgatccttc
    13141 tactttaagc gaagaagctg tgtctccgat tattggatca gcttgacgat cttgccaagt
    13201 catatagccc acgacaacga caatcgccaa agcaaccaat cctaataaaa tgacaggaag
    13261 acttgtccaa aagcgacttg gatgcgattc ttcctgatgc attgcttttc tagaagtact
    13321 tactgtttca ggctgcggac gttcagggac cgatcctgtc aatttgcttt tcccatcaaa
    13381 aacatcgact aaatggtctc catcttctcc tacagcttca gcgtattgcc gaataaatgc
    13441 ccgcacgtaa aatttaccag gcaattggtc aaaatcatca ttgtcgattg cttccaaata
    13501 acgtttttga atttttgtaa tctgctgtaa ttcatctaaa gatatttttt tattcaggcg
    13561 tgcttgccgt aatttttctc cgatggtttc aaaagccact cgttcatctt ctccagtctg
    13621 tttgattctc cgaagcatct gctgttgcaa cgaaagacgt tatgtttggg tgctgtgaca
    13681 aaagtcatgt cacagctcct tttttccgaa taaacattgt tctcctgcca actcctcggt
    13741 cacaagtcac aatatccaat aaatataaga agctgttttt tgaacttgtt ccttgtgact
    13801 cggagttaaa cggcaggctc acgacctctt tcttaaacgg tgttcaatca gctgttcctc
    13861 ggtattagct caaaaaccgg caaaatataa ggagatattt ttccgtatgt ttctctaata
    13921 ctcaggtcaa aacgctgatt tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
    13981 tgcggtaata agtccaacca agttaaaaac attaagaagc tgtttttact tggctgttct
    14041 tattacttga tgcagggcag ctttttcaca acctcttggt acacggtgtt ccagaagtag
    14101 cttcttcggc aataagccga aatatcacaa aaatttgaga agcaattttc gtaaattcct
    14161 tcttatttct tgaagctgac cacttctgtc acaacctctt tgattacttc gtttgatttt
    14221 attttaatat aagttgatta aaagagagca tgatttgttt tattttctca ctaatttttc
    14281 caacacaact aagaatacca taaaagctga ttttggagga ggttcctact acaagtctaa
    14341 aacgaaattt aagacttttc ctcataaata ctggtttatt ttggtctcat gtaaaactcg
    14401 ctgaaggcct cttcacttat aaatgcttcc ccagctgcca gtacgtcttt catttggatc
    14461 gaatcgatga tttcaggaag atcaaataaa gttcgatcgc caaataaact ttgcgtaaat
    14521 tgattggcaa tatattcgat cgaatttaat gattggaaat attgtccaag cattttcttc
    14581 ttcagcaaat cgagatttgt ttctgacact tccggatcat cagcgaatcc aagaatgatt
    14641 tctttgactt tttctgctgc tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag
    14701 tgaaattcac gatccaagct gaattcaaaa ccaaagctgt catcgataat tccttgattg
    14761 tacatcgcta gatagtttcg agaagtattg cctaaaatca tttggaacaa cagattgatt
    14821 gctgttttat agcgtaacag ttctgttcct tcttgcggca atgtgtctaa tcctttaata
    14881 ccaagtacaa atttatctcg tgtaatggct gcttccaatg cactttgttt gatgatttct
    14941 tttgtgtttt cagggaaata acgtacaatt tcttgctttg gagggaaatt ttttgcttct
    15001 tggttttccc gaataagttt catcaatttc tcaggctcca tcttaccaac tacaaataaa
    15061 accatattgc ttggctgata aaacgtccga tagcacgtat acaagtcttg agctgtgatt
    15121 ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca atgggtgtgt aggatacaag
    15181 ttatttagta tgccaaaaaa catccgccag ttagggtcgt cttcatacat ttggatttcc
    15241 tgaccgatga ttcctttttc cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca
    15301 aaatcgatca gagtcgtcag attcttttct acctgatccg tcgtagaaaa cagatagctg
    15361 gttttcgtaa aacttgtgaa agcattggcg gaagctcctt gtttcccaaa taattgaaag
    15421 acatcaccat cttctttttc aaacaactta tgttctaaaa aatgggcaat tccatctgga
    15481 actttcacct tttctttttc accataggga ataaattcgt tatcgatcga accataattc
    15541 gtactgaaca aaccatatgt cttgtgatag tcatttttag gcaacaaata cacggtcaaa
    15601 ccgtttggca gtacttcatg ataaagtgtt tcattgattt gctcatattc tgttttattc
    15661 atttgcattt cctcctgcta aaaagaagat tgcctgcaat tcgatttgct cagctacttg
    15721 ttgtatctct ttgatcgtta cgctctccat acgtttcaac cattcttcat cagataattt
    15781 tgtttccggc agccagctgt ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag
    15841 agaaagcaga tattggtttc tcagcattgc ttttgtctga gacagttcca actctgttat
    15901 ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga tgaagtactt gatttcggtt
    15961 cttttcatcg ataccggttt ggacactcat gaatcctcgg aacgtatcca cgctactaga
    16021 ggcataataa gctagacttt ctttctctct gacattcatg aaaagtttag agtgaggaaa
    16081 accgccaaat aaaccgttaa agaccattag cgcaaaacgt tctggttcat cgtaatacac
    16141 atttgtctga taagctaggt tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc
    16201 cttcacgata ttcacttgag gttgggtata aaacagatct ggataaactg tttcccgcga
    16261 ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac
    16321 aaaaatgtcg atttgatcat ttgccatcat ttcttgatag taggacgcta gcgaactagc
    16381 agtgatcttg tccaaatctt cgactacgcc caaccctggg atcttttgtt ccgggctgtt
    16441 ttcaaaataa agttcttgca aggcatagga agcaaaagtt tgtttatctt ccttcaaact
    16501 ttccaaatat agacgcagat tgttcttttc caagtcaaac gtcaactgat caaactgatg
    16561 atttttaata tttggataaa ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag
    16621 gtttggatca ttgacatatt caccattcac aaaactgatc cctgcgttta gccaatgtaa
    16681 atttcctttt tttctaactg ataatccaaa gcttgcgcca taaagctctg caagttgcgc
    16741 acttaacttt gtctgatccg gataattcaa actatttgtt tccaacatgc tgctgagaag
    16801 tgaacgtttc gtgatgactt cttgttgcag acgcgcagta aaacggataa aaatccggat
    16861 cgttttgtat tgttttgttg gtaagacatg aagattcacg ccttctgcca attgatacac
    16921 catatttata tgctcctttt cacagttgat tgcccgctta ctcttttaat gggtcaacct
    16981 gcctttttta cttattcctc tgcttgaaat ttataagttc aggcaattcg ccgttttttc
    17041 taaatgatgt tacactttat agtatagcat agatgttttt tatcaaaaga aacagacggc
    17101 taaaagcgta tattgttttt ttatttttta tttttcgata tactaaagac aaataaaaga
    17161 agggagggtc attcatgatc aaagaattca aagagttcat catgcgtgga gatgtcttgg
    17221 atcttgctgt cggcgttgtt attggtagtg cgtttacaag catcgtaaac caagtggtgc
    17281 aaggcttgat cacaccacta ataggttggt ttgtcgcaat gatcacaggt acttctgatt
    17341 tagaaggagc actttcaatt cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg
    17401 gtaacgttat tagtgctatc attacatttc tgatcactgg ttttgtctta ttcttagttg
    17461 tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga agagcaagtg gaagtggaag
    17521 aaactcctac tgcagaagat tatcttagtg acatacgcga tcttcttgcc aaacaagtgg
    17581 cagaagacaa tacacatcca gtagagcaaa caaatctttc cgatacttct aaataataaa
    17641 gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt ctttttttag attcttaact
    17701 tgctacttgt tcctttttca tgtcgatatg tggaatatca tcttccaaat acacatctga
    17761 gattgccttg aatccaaacg actcataaaa agcttgtagg tattcttgtg cgccgatcac
    17821 gattggtttt tctggaaaat ttttctggat catgttaatc gtttcttgaa caatctttct
    17881 tcctaaacca tctttacggt tgtcctgatg aacaaggaca cgtccaaaat ggatactctc
    17941 cgcttcttca tatactcgcg tgtaggcaag gatcttccca tcagtatctt gaaaaaacgt
    18001 atgccaagct tgtccatcaa tgtcatcaac ttcttgataa ggacactgtt gttccacaac
    18061 aaaaactgcg attcgttctt tgacgatcgc gtaaaactct gctgttgaca attcttcaaa
    18121 tttctttacc tgatattcca ctaagcaaac gccccctgct tttttattct tagcataccg
    18181 attcgcacac aaaaaagcaa acgaacctta cgaatc
//
LOCUS       DAZKOR010000058        17286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000058 DAZKOR010000000
VERSION     DAZKOR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.058"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..603)
                     /locus_tag="ACG8ZM_001849"
     CDS             complement(58..603)
                     /locus_tag="ACG8ZM_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0492801.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(615..1307)
                     /locus_tag="ACG8ZM_001850"
     CDS             complement(615..1307)
                     /locus_tag="ACG8ZM_001850"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0492802.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1326..1610)
                     /gene="macP"
                     /locus_tag="ACG8ZM_001851"
     CDS             complement(1326..1610)
                     /gene="macP"
                     /locus_tag="ACG8ZM_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0492803.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1623..2186)
                     /locus_tag="ACG8ZM_001852"
     CDS             complement(1623..2186)
                     /locus_tag="ACG8ZM_001852"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0492804.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2414..3076
                     /locus_tag="ACG8ZM_001853"
     CDS             2414..3076
                     /locus_tag="ACG8ZM_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0492805.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3200..4396
                     /locus_tag="ACG8ZM_001854"
     CDS             3200..4396
                     /locus_tag="ACG8ZM_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0492806.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4497..5873)
                     /locus_tag="ACG8ZM_001855"
     CDS             complement(4497..5873)
                     /locus_tag="ACG8ZM_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0492807.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5910..6776)
                     /locus_tag="ACG8ZM_001856"
     CDS             complement(5910..6776)
                     /locus_tag="ACG8ZM_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0492808.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6865..7890)
                     /locus_tag="ACG8ZM_001857"
     CDS             complement(6865..7890)
                     /locus_tag="ACG8ZM_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0492809.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7908..8582)
                     /locus_tag="ACG8ZM_001858"
     CDS             complement(7908..8582)
                     /locus_tag="ACG8ZM_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0492810.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8766..9659)
                     /locus_tag="ACG8ZM_001859"
     CDS             complement(8766..9659)
                     /locus_tag="ACG8ZM_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0492811.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9733..11430)
                     /locus_tag="ACG8ZM_001860"
     CDS             complement(9733..11430)
                     /locus_tag="ACG8ZM_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0492812.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11423..12735)
                     /locus_tag="ACG8ZM_001861"
                     /pseudo
     CDS             complement(11423..12735)
                     /locus_tag="ACG8ZM_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(12735..16676)
                     /locus_tag="ACG8ZM_001862"
     CDS             complement(12735..16676)
                     /locus_tag="ACG8ZM_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0492813.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16785..17243)
                     /locus_tag="ACG8ZM_001863"
     CDS             complement(16785..17243)
                     /locus_tag="ACG8ZM_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0492814.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4832 a         3888 c         3011 g         5555 t
ORIGIN      
        1 tttatgctct tcttttatta ttttgtgctt tttattttat caaatcactt tgttttttta
       61 taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat gtccaacggg
      121 atcgaaactt gccacggaat ctttgaaaat atggagtttg tatcctagat tatacgcatc
      181 gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa tatctgtgat
      241 ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat aatgccgttt
      301 gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt ttaaagagcc
      361 gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc ggttctccgg
      421 atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat ccttttgatc
      481 aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg ctgtcccagt
      541 agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt
      601 catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc tgctgatcgt
      661 ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg
      721 ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg tccaacagcg
      781 gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc tttgatttta
      841 tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc gttcaaattc
      901 gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc ataatataga
      961 ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc atcaaaataa
     1021 gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat tccgccagct
     1081 gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact tgtggtaata
     1141 gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt acctaatgaa
     1201 cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag gttctctctc
     1261 aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt tttaattcct
     1321 tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat aacaataata
     1381 agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct gatccttgtt
     1441 ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat ctttttttct
     1501 tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct tgcttgtgct
     1561 tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat aagccgatcc
     1621 ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt ctttgcatca
     1681 caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg atacaactcc
     1741 agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc ttgtgcatga
     1801 taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt aagatggctc
     1861 agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt catttcggga
     1921 ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac tttttctaaa
     1981 ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg
     2041 acaccgccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg caaacgaact
     2101 tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact gattgttttt
     2161 tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat cataactgct
     2221 tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata gataaacctt
     2281 agattttctc taaatataga aaatccttct ttagtccgat actctttcta gtagtccgta
     2341 tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg ataaatgaca
     2401 tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg tcttgctatt
     2461 tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga taggtgtagt
     2521 tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc
     2581 tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat
     2641 ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa
     2701 tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag tcaaagcagc
     2761 gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag caagtcagtt
     2821 tctttatacg catctgccga atctagttga tttgacaaat aagtataatg agatcaatga
     2881 ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc agatcatcga
     2941 ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg atctagatga
     3001 tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata aatatgaacc
     3061 agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc agatgatgtc
     3121 tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg agaacagatt
     3181 caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg ttacaccagt
     3241 taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag atacgctgac
     3301 cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg ctcgtttaat
     3361 tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa aaatcgatgc
     3421 taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat taagtgaatt
     3481 ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag gagattcctt
     3541 aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg ccggagaagg
     3601 gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag tgacagcaaa
     3661 atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata aagaaaaaga
     3721 cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca aagactactt
     3781 tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac ttcaaacaac
     3841 gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg atgttcagat
     3901 tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg atcttcgctt
     3961 agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa acacaaatca
     4021 ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt ggaaaaacca
     4081 agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc aacgccaagt
     4141 ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa tctcagccat
     4201 cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc aaaccactca
     4261 aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag gaaaagaaaa
     4321 acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac aaaaactgtt
     4381 ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca acaacagttt
     4441 attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat tggagtctat
     4501 ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag tccaataacc
     4561 attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa
     4621 gtcaaaatgg agacgattgg caatagcgga acgaatggca ctttgaattc tccagctttt
     4681 ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat caaataggcg
     4741 agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat
     4801 acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt ttgatcgatt
     4861 tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa acgagcaagt
     4921 gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac agcgatcacc
     4981 gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc attgactcct
     5041 agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta aaggacagta
     5101 gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt tttgacttcc
     5161 tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag agctgcacca
     5221 tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg tttaacgaaa
     5281 aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac catggcatta
     5341 ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa tatcactacg
     5401 agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc agttaatgct
     5461 tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga ccatccggaa
     5521 gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt cagccaggca
     5581 gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac tggcactcgc
     5641 gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc taagacaaat
     5701 gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac cacaaatatt
     5761 cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt tagttctttt
     5821 ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc cattgaagtt
     5881 cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc taaaaggata
     5941 ccacctaaaa tgactaaaag gcaaccaaca ataacataaa tgagctcttt tttcgttttt
     6001 ctttcgccaa ggatgaaaat cccacctaaa gtagagataa tgatccccat ttgtgaaagt
     6061 gagaagccaa cagctaaacc aagtgatttg actgttagaa gcatgcagat gtttcctaag
     6121 ccccataata atccacagat catgtttttc cacacaaact gatcgacttt aactttacgg
     6181 aaagcaaaga agcttgcgcc taagatcatt ccgatacttt gaggtaagat aattgacatc
     6241 gcatccaaat tcgcccagtt gacgatgatg gtatatacac cataaccaat cgtagagaag
     6301 atcaatgctc taaaaccctt accaaaatca agcattttat tatctgtatc tatttttcct
     6361 tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa gaagagctaa agcaataata
     6421 ccgtatacat aatctttcgt ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta
     6481 ttcaaaatta attgaaaacc agtagacaat ggtagcccta cagaaacacc catgtacttc
     6541 attccatgga actgtccatt ttgtccaaca ctccaagcta agccggataa aaatccgatc
     6601 aagaaaatcc aaggggtgat tacaggacta acaacgaaaa agacaactaa agaaaacaga
     6661 aatgcaccga tcgtcattcc tagtgtttgt tgattggcat cgccaccaat tttaccactt
     6721 actaaaccaa tactacccca tgccaacata gggactaatg ctattaaaat ttccataacc
     6781 aatctccttt tatttcacat gataagaaac ttatcagcga aaacgttaac aacacaagca
     6841 ctttttttcg tttctaacga ttttctaacg tttttcatta taaaaattta agattgggaa
     6901 aaataaagtc attgttgtcc cttttccttc agttgattca gccgtcaaat ggatacctaa
     6961 tttttcggcc agattttttg ctaaataaag tcctaatcct gttgaatgtg ttttgctaag
     7021 tcgaccattt ctcccagtaa atcccttatc aaagatccgt ttcaagtctt ccgctgggat
     7081 accgatccct gaatctttaa ctgaaaggct gactccttgc gcttcctttt ctatgctcac
     7141 aatgatgttt ccgtgatccg gcgtatattt gatggcatta ctaagcaatt ggttgaagat
     7201 aaaagcgatc catttagcat cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg
     7261 aatatttttt tggataaaat aatttgcctg actgcgtatc acaggctgaa tgattccttt
     7321 tagtccatat tcttggatga ggtagtcccg cgagaaactg tcgagtcttg cataatacaa
     7381 aacttgttct acgtattcat cgatttttga taattcattt tctagtaatg tatacttttg
     7441 atcgtcaata tcgaactcga tcgaacgaac tagcaattta gaagcggaaa gtggcacttt
     7501 gatttcatgg acccatgagt caatataatc tttttggtct tgttgattac tgattgctga
     7561 ttgcattact tcttggtgtt cacggatcag ctgattgata tattcttcta ccagtttttc
     7621 ttcttctgtc ctcccaccac tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc
     7681 taattttccc caccatcttc tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa
     7741 gacgccctca atcaaagcta aataagcaat tgtgctcaaa tcaactttga tgtttggcgc
     7801 cagccacatc acaaagatcg tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca
     7861 ctgatctttc aaatatttaa aaaaattcat cttaaccgct catcctttca gggaacgata
     7921 tatccctgtc ctacttttgt ttcgatatat ccttggatac ctgcctgttc gatttttcgg
     7981 cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac
     8041 agtgctcgca gcaatttttc ccgacttaag atctttccat tgtgcttcat caaaataaat
     8101 aatagacgat attcattttt gctcaaatca atgatctctc cgttgatttc catacttccg
     8161 ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt cactagataa ttctgtgtag
     8221 ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat ttggaaatgt
     8281 tttgtcacaa aatcatctgc ccccatgttc atagccatga tcatatccat attcgtattc
     8341 ctgctggaga taaaaatgat cggtactttc gatacttcac gtattttttg acaccaataa
     8401 tagccatcca aaacgggaag attaatatct agtaatacaa gctgtggttc ttctctttca
     8461 aagtctgaga acacttgatt gaaatcggtc gttccaaagg tttcaaattg ccatttctca
     8521 agttcctcca tgatcaattg tcgaatgatc tcttcgtctt cgactaccat gatttttgcc
     8581 ataaaaaatc cctccatctt tcttcttgct actacaatat caaagaagcc cttgtgaaac
     8641 aagttggctt acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg
     8701 atttaatcgc gtttgtctct tccaagcagc ttaaatttat tatgttattc ttaataattg
     8761 gtctattatc tgttattttt ctttcgttgt ttttgtcttt taaattttcg ataacaccaa
     8821 taaccgaaaa gcgttagcac tagcaatata cctagaagga gtataagtaa tcgataaaga
     8881 tggtattttt ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg aactcgtctt
     8941 cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact
     9001 agatcctgct tttctctaat tgctaatgta tttgtttttg ttggaaggac aacaattttc
     9061 tcaacgactt cataagcgag catccgacca tacacttcaa gatagaatcg gtcatcttgc
     9121 tccatttctt caagatccgt aaagagtttt ttctcagcaa taccgctatg ggcggtaatc
     9181 acggaatgtg tactctctcc tccagttgga aaagaggtgc cttgcaggac tgtcgctcct
     9241 ctatctaaca aaagatcatt cgtttcatca aataaaggca agctaacatt gattttcgga
     9301 atatagatgg ctccgatcat atgttgttcg taatattctt ttcctggatt ttggacattt
     9361 ctaaccgctt gctcaaaagg atctttgact tgtcccattc ctgggattac tgatgtatga
     9421 gcaagcgtct gatttttctt ttcatgcact gctaattgtt gctcatgttt ttcttcttta
     9481 tcgcgttcaa gctgcttttg ataattctcg atccttcttt gatccaaata attgctgaga
     9541 gcatctgcta aaaatggata tgtaaaaact gcagctccgg caagaaacat cactgccata
     9601 aataattgta ataaaagact gatttgggtt tgaggtctat tacctatttt ctttttcatt
     9661 ttcttcacct tcttatttaa aaaaactgca gcaaccttta taaaaccgat tgctgcagct
     9721 tatttttgtt gcttatactt cagacttcat tttagattta cggtaccagc tgtacgctcc
     9781 aatcatcaaa cttattccga ttagtaagaa tgccaagagc ccgttcccac cagtagcagg
     9841 aagtgacccg cgtttcatat tcggtactct ttctggccct ggattttcta ctcctacgat
     9901 ctgaatttct tcattatagc ttccataaga gatgataaat tcagtaaagg ctgcctcacc
     9961 tgttggaagt acataacctt ctggcgccat ggtctctttc atttgatagg ttccataatc
    10021 taacccttga acctcaaagt atccatttcc atctgcgaca aattctgttg cttgtccgct
    10081 tgttgcccac tcaattctat ctgctcctga ttcataaaca gctaaaggcg actccccttt
    10141 ataaaatatt gcgtattcag aacggtcttc attccacaat tcgaatcttg catctttaag
    10201 caattcactg cttgatgcat catatttttc aaactttctt ccacctgttg tgactttctc
    10261 tggcggctct actccttctt cataatcaaa ttcatcattt cgtccgattt caataaccgc
    10321 tgtgttgtta tggaaatcat ccgcttctag ttctgtggaa accttcaaat tatacgtgat
    10381 cgttaaatct cctccacgtg catctgctaa tctacctaaa gtatctactt tggctactcc
    10441 attttcttct gtcaaaatcg tcacgacaaa cccgttgctt actacttcta ctatataatc
    10501 ttgtcctttt gttaaaatcg tctcgccggc tcgcacttct atttcctgat cataataagt
    10561 tagaccaact gtaggcgtat ctgtgatttt gtaatttttg ataacagcgc ttccgttttc
    10621 aaggacagaa tcgatgaaat aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc
    10681 tctttctaga tcaatataag aaatcgtttc ttgttctcca gcttcattaa taatcgttac
    10741 ttgtctaagg tcgctttcct ctacattcat ctgtttatct gcttctctga tttcgttttt
    10801 cggatacaaa tgcacatctg tattgtatgt gtctttccat gtagaaccat cccacacgcc
    10861 atccaccata tcaggcatca tcactggcat cataacaacc attggagacg cgattctgga
    10921 aatatgagct ggcgaataag tttctaagaa aagatagact ttatctctag aagaaggtgc
    10981 ttcttccgtc acttggacgg tagagaatac cgcttctcca gcttcattcg ttgtttgttt
    11041 gtcaatactt gtcagtcgat attttaaaaa ccaactttct gtgtgccgtt tttgaatcca
    11101 ttcaatcgct tcggcagaat taagaccgtc ttctcgttcc ggtgttaaag gattatctcg
    11161 aatcaaatcg taatatactt ccgttacgtc aaacaaatca aaatcgactc ctggaatatt
    11221 ttcttcacca aaatccgtag aaaccaatcc gctattcggt cgttcttccg gaacagatga
    11281 gaaacctttt ttgtgtaatg taatcgtcac ttcacttggt ttttgtgcga cttcttcggc
    11341 tttgactagt tttccgttcc cgaaaatccc tattcccgca gtaacgagta acagtacact
    11401 cattcccgtg acaatcattt ttttattcat ttttttctcc ccgttctttt tttattaata
    11461 aaacagcacc taacaataaa agaattccta gtaaagaaat caacgttctt gcttcattcg
    11521 tttgcgggaa tacggaccat tttcctgatt gacctggatt gcttggacca ctcggctctt
    11581 tcggtttgtc cgttgtttct tttacggtct cgttataaac acgtggtgtt gcggattcat
    11641 aggcagagac tggaactttc ccggattcta attcagccat ctcctgtccg ttaactacta
    11701 catattgtgg ttggcctttt gcatccaccc aaaattcagg gatgatgacc tcgattctct
    11761 tacttgcttc atccatctcg taaccttcca caccttttaa ttcttcaaaa taataggtgc
    11821 cagaaggcaa gaaacgttgt cctgtcgtaa caaggccctc tttatccgaa atgaatttgc
    11881 ttacattttt gtcattcaaa ggatcattgt ctgctggttt gatccattga tttttcaagt
    11941 cattagcagg agccatatct aaataatatt tcccccttct atcatctgat atagagcaaa
    12001 aacagcacct tctaatggtt ttcctttttc agttgttcct ctttgacgtc catacttgaa
    12061 gaagtatgga tctcttagat aaccgacatt tttaggatag atatgaatct cttgcagttc
    12121 ctcggttgat tctgtcgggt gataaattgg caaaatagca gcaattggga tagctgtctg
    12181 atcgatatca acatccaatc caacttctgg atcaatcgat tcttcgaaaa tcaaataaac
    12241 tgcatcccga tcttggtttt tcttaggtgc tttgatacgt gcaatcccgt ctcctaatcc
    12301 gccaaccgta tctttcccag ttgttaccga agtccctatt tggacaagcc tttcttctgt
    12361 gattagctga cggatattgt ttctgttcgt ttcagcgatt tgctggacga attctttagg
    12421 agtcatgttt tcagcagctt ttttttcatg ataataatcg gtcatgtcgt aaatggcaaa
    12481 tgtcgctcca tttagtggca ccgtctgtga aatgatatct gctgtttccc ctgcttcctc
    12541 agagatagcc aagccatcat tttcatggta ttcaaattga tcgttataat cgatatctcg
    12601 aatcatccgt ttatgcaaca cgaattccac gacatcttcc cctgattctt ctgccgacat
    12661 cgcatgtgga agaaggactg ctgcaagcgg tagtaaaaca agtaattgaa gcatcaaaca
    12721 caacagcttt ttcattacct tgctccctcc tttttagatt gtctgacaaa caaaccatag
    12781 gctgctcctg caagcgccat gaagaatgca gcaagcagga tatagaataa tcgtccggat
    12841 cctcctgtag caggcaacgg attttcagga tcgttcccga cactaagctg taatctgatt
    12901 cgatttgtct ccaaaagttc atattctaac tcttgctgta gatcaggcgt ctctgcttct
    12961 tcgtttggat aatacgaaat ctcaactttt agtgtacccg cttcttttcc tggaactacc
    13021 cgatcatttt ctatgtcagg ttcttctcga taactaattt ctaaaataaa gtaacctgga
    13081 agttttctat agctgtcagg tgacttcact tccttgatcc gatagacccc ttccaacaag
    13141 tctgaagcat tgaataggga agaatcgttt ggatctggtg ccaagcctcc ctcgatgatt
    13201 tcatactctc ctgcactatt ttgtttctca attgaaaatt ctgcgccttc aagacgtcgg
    13261 ctattcatac tgtcaatttt caaaagttcc aaatcgattg gcgctagttc atcttctaag
    13321 gttacttgta gtacaccatc gatgatttca tagccttccg gcaagggagc agtttcgtct
    13381 tcgatgaccg tatcttcaat gataaactta ccttcttcat tcagtgcaaa ctggaacact
    13441 tgatcagata gccgataccc atttggtgcg acagattccg agacctcata ttcaccaata
    13501 gacagatgca gaggttggtc gtaatttaaa tcgacatact tttctactcc tacggcacga
    13561 gaatagaagt atccgatccc aggcaaacgt ttcgattctt ctggttctaa cggattacca
    13621 gtgtatgctc gatacagcgg gaaggaagaa tcccccactt tcctgatatc gaaacctgca
    13681 ccatctaatg tatggccttc atggtctaat tttctaactc ggatctcccg ataatcaaac
    13741 gtattgtaag cagtgaatgc tacccataaa cgatcctctt catctgttgt atacgtgaga
    13801 tattgccaat tttcttcacc tgtttgcttg agttggatag tccctatagc attatcgtaa
    13861 agtcgcaaga tccatgtgtc attttcatca ggttctttga aaccttctgg gttctggctc
    13921 tcaatgattt tgtattctgc atagttggta gtttcttcac tatccttata aacaaatgaa
    13981 tctggctctc cgattggatg aataactttc tcaccatcca cgattacata tttcccgtct
    14041 tcatctactg aatagaacgt gagtctccct ttctcttcct ctacttcgtt cacaccagaa
    14101 agccctttcg ctactggata tccgatttcc agtccgttac catctgtttc ataaagttca
    14161 aactctgcgc cattgattgg catattactg ccttcaatta gtttattgat attcatgtgg
    14221 attggcttga attcatttcg catttccagt ttgacataca actcatcata ctctgtgcct
    14281 tctatgttag gctcttgtac aacagaaagc ttgtgatatt ctccttcata ataggaattc
    14341 ggatctttac cttccggtcc gccgactctc gaaccagatg tatattcatc tacaagctcg
    14401 tagtgaccta acatctcatc ttcgttggct gcatattttt tgatttggaa ttgatacgtc
    14461 tcatctacta actgataacg tggtggtcca gcgatctctc tgatccaaaa tatgtcgctg
    14521 actgctccag tttcatctcc taaaatcggc atcttcgttt caaagatcgc tattccgtcc
    14581 gtggtagttt tacttgtttc gattggttcg gcttccccac tctctggatt ttggtcatat
    14641 aatgcaaatt cgactccagc caacggctcg ccattttgac ccttcttgct gacttggaaa
    14701 ttacggcctt tatgttcgta acgaatggaa gaagatggga aatacatccg tccagtagtg
    14761 atatcattct cataatcctc caggttctga atataggcca tattgttcac tgtgcaaaaa
    14821 tttccgtctt gagctcccgc atcaagtttt acttggtact tgatcctgac catttcccct
    14881 tcacctaaag aaatcggaga aaatttataa gcttggtttt cccgtccgac atacgttgtc
    14941 ggagtcgcca ctacttctgg tggggcttgt ccatgacgtt cttcatccca tatttctggc
    15001 tcttggtact tcatgccgtt ttctcctgga tctagatggt aaaactgaat atctgccgat
    15061 ctgccagctc caaatggtga gacattaaaa ccagtttcat cataaagtgt ggcttgctgg
    15121 atcgttctct tgaaggtaga agagatatcc tccaaggctg taacaatgtc attcttgttg
    15181 ttcgcattgt aatatcgcgg tgttacgcca tcttcttcta aatcagcaat atttttcaat
    15241 acatttcgtc caatagcatc tagtcgttgt ctcccgctaa cgtttccatc gattcctaaa
    15301 ccaatggaga agatacttgg cgggttctct tgatacttgt ttttcagcca atgatgataa
    15361 gcaactgtgt tgatcgcaga tgacttttct tgcgaagtag gactgccatt cgtattgtta
    15421 ttagaagaac catcaccaac ccagtgagtc gctttattat ctccttcttt tacctctcca
    15481 taacgatagt gtacatttac ttcatcttct ggacggttaa agtcagatgc ataggtaact
    15541 ggatgagtaa accctgcatt agaagtattc ccagaaaact cctcaaaatt tctgaacaaa
    15601 acgtcattat cttcagcaat cttatcttcc atcacagtcc agttgctcca agctccatta
    15661 gtgctcgtac gataacttga ctgaatcggt gcataactga aggtcggtgt cccatctccc
    15721 acaacgatta ggattttttc tggatttcta ttttctccgc cattgtcttt atacaatgtt
    15781 tcatagccat ttttaagccc tagtgtaagc ggagttcctc cgattggtgt acgatttaat
    15841 gctgtattct cacttcccgt caaacgattg atatcatttg tcaatggaaa gatgtccgaa
    15901 ctgatttgct catggttatt gatagctgta ctattatggt aaaaattcac cataccgatt
    15961 cgaatatcca gactcgggtc agagagcaga tctttactca tttcgtttac tgcctcaatc
    16021 aacgcagcat ttttagtttg actattggtc ccagcggtta attcactcat actcgctgac
    16081 ttatctagaa caaaaacgat atcgattgga ctgatttcct ttttctcgcc cccgatgaca
    16141 tctagataga cattgtaaag ggttgttgca tcttcaggat gtagatcaga tgctgtgctg
    16201 cttggcagaa caggttctac ccattttttg atgtacgccc cgttataatc caagtaactg
    16261 ttttcaaaat tccgttcatt tccccaaagc tgaacggttt gagctggaac gtttctatct
    16321 tcttcgcctg cagttgatcc attattagta gcatagtcgt aatttcgcca gttcgctccc
    16381 ccgtttgtat aatcactatc atcgatcaag ttctctccgt catatggcga gcctttgtct
    16441 ccttgatagc gttcattgct ttgtgtccaa tttcctaaca cttttgtata gtcattcgtt
    16501 gtaaacttcg ggtagcgatg ctgttcgttt tcaacttctc cgctaggata gatatattca
    16561 taggggtctt gcgtagctgt tggcccaaga cgctccaaaa taccgttgaa taactggcgt
    16621 tgaatgcgct ggagtgcttg accgctgggt acgattggtt cgtccagctt ttccatctca
    16681 ttttcttcgc tacgttgaat ttcagcggaa tcttctgtat cagtttcatt ttcttgtaat
    16741 tcttggttat ctattctttc atcaaccacc acttgaagag gtagttaccc ttcgttcaca
    16801 tcggggatct tttctgtttc aaaaatcaat gtgccggttt ctgcttcttt tgaataaact
    16861 tttccgacat accattcttg accatcgatc gtctcgactt ttaggtcttc tccatcttca
    16921 tcataggagg atagacctgt tccacgaacg ttttttacgg taccaatccc ggcggctgcc
    16981 gcatcgattg ctaaacgtat tctaccttca ttggcacttt cctttttttc aaaagccaga
    17041 tgccattcaa gggtttcttc tttttcaata tacttccctt caatttttgc atagtcctca
    17101 tctaagaata cctcatcctc ctttgtttct gttgactcag cagctacatc cagggtcgtc
    17161 atatgataga ctgactggaa atatggtcca aatagcaaac tgacagttac taactgcaaa
    17221 agcttcaatt ttaacccttt cacatcctta tactcctttc aaaaatattt gtcttctttc
    17281 acaaca
//
LOCUS       DAZKOR010000059        16761 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000059 DAZKOR010000000
VERSION     DAZKOR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.059"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..295)
                     /locus_tag="ACG8ZM_001864"
     CDS             complement(<1..295)
                     /locus_tag="ACG8ZM_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0492815.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYG"
     gene            complement(297..494)
                     /locus_tag="ACG8ZM_001865"
     CDS             complement(297..494)
                     /locus_tag="ACG8ZM_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0492816.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(888..2615)
                     /locus_tag="ACG8ZM_001866"
     CDS             complement(888..2615)
                     /locus_tag="ACG8ZM_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0492817.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2608..3801)
                     /locus_tag="ACG8ZM_001867"
     CDS             complement(2608..3801)
                     /locus_tag="ACG8ZM_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0492818.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3988..4632)
                     /locus_tag="ACG8ZM_001868"
     CDS             complement(3988..4632)
                     /locus_tag="ACG8ZM_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0492819.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4726..4860)
                     /locus_tag="ACG8ZM_001869"
     CDS             complement(4726..4860)
                     /locus_tag="ACG8ZM_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0492820.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4862..6994)
                     /gene="feoB"
                     /locus_tag="ACG8ZM_001870"
     CDS             complement(4862..6994)
                     /gene="feoB"
                     /locus_tag="ACG8ZM_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0492821.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6991..7464)
                     /locus_tag="ACG8ZM_001871"
     CDS             complement(6991..7464)
                     /locus_tag="ACG8ZM_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0492822.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7865..8089
                     /gene="nrdH"
                     /locus_tag="ACG8ZM_001872"
     CDS             7865..8089
                     /gene="nrdH"
                     /locus_tag="ACG8ZM_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0492823.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8248..10407
                     /gene="nrdE"
                     /locus_tag="ACG8ZM_001873"
     CDS             8248..10407
                     /gene="nrdE"
                     /locus_tag="ACG8ZM_001873"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0492824.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10457..11422
                     /gene="nrdF"
                     /locus_tag="ACG8ZM_001874"
     CDS             10457..11422
                     /gene="nrdF"
                     /locus_tag="ACG8ZM_001874"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0492825.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11511..12650)
                     /locus_tag="ACG8ZM_001875"
     CDS             complement(11511..12650)
                     /locus_tag="ACG8ZM_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0492826.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12959..13672)
                     /locus_tag="ACG8ZM_001876"
     CDS             complement(12959..13672)
                     /locus_tag="ACG8ZM_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0492827.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13926..14627)
                     /gene="nagB"
                     /locus_tag="ACG8ZM_001877"
     CDS             complement(13926..14627)
                     /gene="nagB"
                     /locus_tag="ACG8ZM_001877"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0492828.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14861..16393
                     /locus_tag="ACG8ZM_001878"
     CDS             14861..16393
                     /locus_tag="ACG8ZM_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0492829.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16584..>16761)
                     /locus_tag="ACG8ZM_001879"
     CDS             complement(16584..>16761)
                     /locus_tag="ACG8ZM_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0492830.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5203 a         3452 c         2886 g         5220 t
ORIGIN      
        1 ctccataatc tgctcgatat aaaccattta ttccagcagt tcttccaatt tcactcaaat
       61 ttcttattaa ttcctcttga tattcagtga atcttatttt attagtataa gcagtaaaat
      121 atagataatt caccaatcta tttattaaag gactgggatc taattcatat ttttgttttt
      181 ctaattttaa atataccttt tgtaaaacct caagaatatc agaatattta ggatctttac
      241 ttatagatat atcattatat aattcatgaa caatttgctt agcatttttt ttcattttag
      301 cacttccctg gaattgctcc acctaaaaaa ccacctatag acattcctgc aatacaagta
      361 gttgccttgg cccaatcgac cgtacattta tttttagtgc aatacactcc atttccataa
      421 tattttccac tatgagtggt acccccatag ataagttgtg tctctttaat agacaaaatt
      481 tttaaatgtt tcataatcta atcaccccta aattaatgtt tttagactat tattctaaca
      541 ttcattaaat ttcaaaaaac aattccttct tgaaacgtag caaaataatt cctgaataag
      601 aatttaatca cttttaagta tataaactat gtatattctt taatattttt tgagaatgca
      661 cttcgtacat aataaacttt aatcacatta gtctttcata cctacgatcg atctctttga
      721 ctcgcaccca taaattcata atagtttgtc ggcgtctttg aacgtttcga agatgagcta
      781 cgcattctct ttttttatag acaaaaaaga tagagacgaa catggagctt ccaaatttgg
      841 atatttgatc caattttgga agctccattt atttcgtgtc tgtcttttta ttttttccag
      901 ctttcgacta attcttggtt ctctttgatc cagtcttttg ctgcttgctg tggatctttt
      961 ccattgttga tttccaacat aactttttcc atatcttttt ctgaccaatg gaaattgtct
     1021 aatactttgt aagcttctgg ctggtcttct ttcaatcctt ttcgaaccat cgtatggatc
     1081 tgctcttcgc ttcccattcc gttttcagga tcttctaagt atttcagatc atatttggca
     1141 aacatccaat gaggtgtcca tcctgtaact acaattggtt cgtgtttttt gatcgcttga
     1201 cctagtgcaa cggtcattgc tcctgaagaa gatgtctcta ctttccagtc tttcaagttg
     1261 tcatattttt ggatcgtgtt ttcagcagcc gtaactacac cagctcctgg ctcgattcct
     1321 atgattttct tacctgcctg atcggttaac tcatcgatcg aatttacatc catgtaggct
     1381 ggcactgcta aaccaacttt tgctcctgtc aaattggcac ctaggtcttc gacttgatct
     1441 ttatattgtg catattgtga accatgagtt ttaggtagcc aagctgaaac catggcatct
     1501 gactcgccat ttgaaacaga tttccacatg attgaattat ctaatggcgt catcgttaca
     1561 tcatagccca tttgtttcaa tacttcaccg actacatttg ttgaagcaac ttctgtatcc
     1621 cattcaacat aagataagtt gatacgtttt gtttctttgg cagatgaaaa actccctata
     1681 ccaatcgcac ctaagatcac tactactaca gcaccaatga tcagtccttg acgtttttta
     1741 cttttctgtg ttgttgctcc tgcagctttt ttattgtttg gctggttcaa atgctgtgta
     1801 aaacggtcaa tgatgattgc taagataact aatgcaaccc cgttgacaaa tccattcccg
     1861 acttgtgctc tttgcaatgc tgataatacg ccgcgtccta agccgggtgc accgatcata
     1921 gaggcaataa cgaccattga aagtgacagc attgttgttt gattgatccc tgccatgatc
     1981 gtacttttag ctaaaggcaa ttctagtttg aatagttttt gacgacttgt gctcccgaat
     2041 gaatcagaag cttctactaa ttctttagga acttgtcgaa tccctaaatt cgtaaaacga
     2101 acggttggtg gcagagcaaa gatgactgag gcaaataccc ctggaaccat cccgataccg
     2161 aaaaatgcaa ctgctggaat caaataaacg aatcctggca ttgtctgcat aaagtcaagg
     2221 atcggtttga taatactttg ggctttactg ctttttgcca tcaagatccc taatggtacc
     2281 ccaatgatga tcgagatcac actagataaa agaacaagtg tgacagtatt catcaaatca
     2341 ttccatagac cttggttata aataaaccat agaccgatta atgtgaacaa cgtaagtcct
     2401 ggacgtttct tagaaataaa gaaagctgct accgtcaata acaagataaa caaaggagct
     2461 ggaatcacta acagtaaatt tgtaatcccc gacatcactg cctgtccaat cgtttgtaaa
     2521 aatgaaaata ggccagaaaa agttgtcgta aaccattctg tgatattttc tacccagttt
     2581 gccactggta attgatagtt caatagatta ttcatgttca ctcacctcgc ttgtttctgc
     2641 taatgcttca atcacactac ctcggatgac gacacctttt aattttccat cctctacaac
     2701 tgcaagtgga gtcggtgagt cataaataat attgaaaata tcacttacca ccatgtcttt
     2761 cccaatcacc gtcacatttt catctaggaa gtcgatcaat ggacgttttt gcttcctagc
     2821 ttctaacgct tgatctgcag tgatgatccc tttcaggtgt ctctttctgt caacagccat
     2881 cagcatgctg acttcttcat tacgcatcct tgtcaatgca acgtttggtc catcagattc
     2941 gatattcgtc gtcaatgcag gtaccatgat gttttgagcc gtaagcactt tggaacgatc
     3001 gacttcttcc acaaattcac gtacataatc atttgctgga ttggtcaata tttcttcacc
     3061 tgttccgatc tgcatgattt cgccgtcttt catcaaggcg attctgtccc caatccgtaa
     3121 agcttcattc aaatcatggg taatgaaaat aatcgttttt tgaacattgg cttgcaggtc
     3181 cagtaattcg tcttgcattt ctcgccggat cagcggatca agagccgaga acgcttcgtc
     3241 catcagcaag atctcaggat catttgctaa agctctggct aggccaaccc gttgttgcat
     3301 cccaccagat aactgactag gatattggtc tttaaatgaa agcaaactag aattttctaa
     3361 tgctttttct gctttttctt gtcgttcttc tttaggtaca ccacggactt ctaacccgta
     3421 ttctgtattt tccaagatcg ttcgatgtgg aaacagaccg aagttttgga aaaccatatt
     3481 gattttatga cgacggactt ctcgcaattc ttctttgctt aacttcgata catcttctcc
     3541 atcaatatag atactgcccg aagttggctc aatcagacga ttcaatagac ggatcaatgt
     3601 ggatttccca ctcccggaaa gccccatgat cacgaagatt tctccttctt ttacgtcaaa
     3661 attcacgtca tacacaccaa ccgttgcacc ggttttcttt aaaatctccg ttttcggctg
     3721 gtgatcattc atcatatcta aagcggcttg tgtcttttta ccaaatatct ttgttaaatg
     3781 ttttacttct acttttacca aacaaattct ccttttttat gtaaattcat ttttctttct
     3841 gaaaaaagtg accacactat gttaacatca tgtagtcact ttgtcaaaaa tgaaagaaat
     3901 ttgcttgtcc aaactgaaaa aaacttggct aaatctttgt aaataaaagg tttagccaag
     3961 ttaatatttt tttaatattt cacacaatca ttctgcaagc tgatgctccg ggtaaaaaaa
     4021 gtttgtcata cgctgtagtg tatattcatt tccaataaaa tgaacgatgt cccctgccgt
     4081 aaatttagca taggggccag gtgaaacaag cagttcttca ttatgagaaa ttgcgataac
     4141 tgtggcgcca gtcgcctgcc agatattcaa ctcgctaatt gaacggtcca gatgcattgc
     4201 tttttctgat aaagataatt caaacggaag taaaggattc atcttatgta ttcgcttcgt
     4261 ttgattgacc agctgatcca ataattgaga aaaatgattc agctcatctt gttgcttctc
     4321 cacacttgcc atgatttctg cttttgtatc ttggatggat tgtacatctt gatattgcaa
     4381 taggaaatct tttgcttttt ccttcgaagc aacaaatgcc ccacttccat gccgtacttc
     4441 catgattcca agatctacca gcacattgat agccttgcgg gtagtctcag gagaagcatt
     4501 gtacgtactg gcaagtgttg agcgggcatg gatcttctca cccactttat atcggttttc
     4561 aacgatacgt tcggctaaat caacagcaat ctgctggtag cgcgggcgga cgattttttt
     4621 ctttccagac attttctttc ctctttcttt ttttactttc cctcatctta ctatgctcta
     4681 gcaaaataag caaaaaaggg gagaatagcc tcttgcaacg actgatcatc gcttgatctg
     4741 ttcctttttt actgggcaag ctgtttgaca gtcatcacaa ttcttaccgg attttaaacg
     4801 agtatagaca attgcgcctg cacttccaaa gattaacaca cttaataaga atgtcgccat
     4861 cctattcctc tccttctaat gacaatatag tataaaactg gttcttggat tttggctgac
     4921 ggaacaaaac ataaacgagt cctatcatca gtaaaatagc aagatacgtc cctaacgcta
     4981 cctgtccacc ctccaataag tgtcccaact gataaatcac aaaactaact aaataagcta
     5041 aaccgcattg atagcctatg gcgatccaag tccatttctt ggcattcatt tcccgatgga
     5101 tagccccaat tgccgcaaaa caaggcgcac aaagtaaatt gaaggctaac aatgaataag
     5161 ctgctgcggg agtatatgtg tgttgtagca cttgccagat ttctctgcca ttttcggata
     5221 cttccgcatg accaaacaaa ataccaaatg tgccaataat attttcttta gcgaccaatc
     5281 ctgtaatcgc tgcgactgtt ccttgccagt ttccccaacc aagcggctga aaaacaactg
     5341 ccaggattcg cccgaaaaag gctaagatac tttggtcttc agaaacaggt tgagcataaa
     5401 aattataaga agagctgaac cagatcacga tactagaaac aaagataatc gttcctgctt
     5461 ttttaacaaa agacttgctg cggtcatagg tttgtcttag gaccgctgac cattgaggaa
     5521 aatgatatgc gggcaattcc atgataaaag gagctgtatc tccagcaaac aaacgtgttt
     5581 ttttcaagct gattccagag aaaataattg cagtcatacc aagaaaatag gcagaaggag
     5641 ctacccaaga ttgatgagga aaaaaggccc ctgctactaa ggcaataatc ggcaactttg
     5701 cagaacaagg catgaaggtt gtgaccatga tcgtcaaacg acgatcttgc tcgttttcaa
     5761 tcgtgcgact ggccatgacg cctggcactc cacatcctgt agcaatcagc ataggaatga
     5821 aggattttcc tgaaagaccg aatttcctga aaagtcgatc catgacaaaa gcgatcctcg
     5881 ccatatatcc gcagtcttcc agaaagctca agcataaaaa caatataatc aattgcggta
     5941 gaaatcctaa cactgctccc acgcctgcga tgatgccgtt caagatcagc tcctgcatcc
     6001 atcccgctac ttgccattca gctaacaatc tgccaacatg ttctggtaac catgtaccaa
     6061 acaatgtgtc attgatccaa tctgtcccca ttgttcctac tgtttgaata gccagataat
     6121 agatcagcca catcacaaat gcgaagatcg gcaaggctaa ccaacgattc gttgcaatct
     6181 gatcgatttt gtcactcatc gataattgga aagaatcatt tgacacaaca cagagtgtac
     6241 atagttgggt aataaattca taacgttcat tgaccagaat cgtctcacta tcgtcgtcca
     6301 atagtttctc tgttaactgg atcagttgct cgatctcttt ttgctgagaa actgctatct
     6361 cgtaatcctc tcttaccttc tgatctcgtt caaaatattt cattgcatac cagcgtttct
     6421 tgccgctttg ttctcctagt acatcttcaa tttcatgtaa agctgcttct aaacgtggat
     6481 catatgtaca gggaacagga atagaaggcc gcttttttgc agactcctga atcttttcga
     6541 ttagtttttc gattccttgc tttttcaata cgctgattgg taaaactggt acaccaagac
     6601 cataggaaag cttttctaga ttgatttttt ttccttcttt tttgatgaca tccatcatat
     6661 tcagaccaat gatcatagga atgtctaaat ccaataactg cgtagttaaa taaagatttc
     6721 gttctaaatt ggtagcatca ataatgttga tgatcagttc aggctgttcc tgaaccaaat
     6781 aatctcttgc aatcacttct tctggtgtat aaggagacaa agaatagatg ccgggtaggt
     6841 cctgaatatg aatgttcgga ttttttttca acgatccttc ttttttttct actgttacgc
     6901 ccggccaatt ccctacatat tgattcgaac cagtcaagag attgaacaag ctagttttcc
     6961 cgctgtttgg atttccgact aacgcgatat tcattctttt tcctcccttt gtatcaagat
     7021 tagttcggcc tctgactttc gtaacgtcag ttcataccct cgcactgtca gttcgattgg
     7081 atctcccaga ggggctagtt ttcggataac tatttttaca tttcgcgtca atcccatatc
     7141 cattaaccgt cgcttcactg cgccttgtcc gtggattgct acgacgatcc cagtctctcc
     7201 tgcttttaat tgatcaagag aagtccagct ttcagaggaa gcagtctttt tattgacaaa
     7261 gatttgacct agtatttccc gacttactgc cactcgatga ctttttacca gtaaaatggc
     7321 attgttgcct gataaattga cgacgacgat tttacttccg gcaacgatcc cgatattttg
     7381 caaatggcgt tttgtttttt cttctgtttc gattcgttcg acttgataaa cgaaattctg
     7441 ctttgcctgc gctaaggtct gcatattgat tcctctcttg tgaaataaat catgtattaa
     7501 aatcattcat tatatgttct aattttctta cgtgttacgc aaataaatga gaatcattct
     7561 cacatagaat actctcggta attgagaatg tcaattatac ttgtgatatt atgagcgaat
     7621 ctttagtatt gatagataaa aatgagaaaa aaatatttct tcatagtttt ccacaatttt
     7681 ttctctatta aatttgctat tttcccacac gatacttgtt ttcagctctt ttttcaaaaa
     7741 cacaaaactt agtattccac agtttccaca acacaatatg tagtgtttgt tgtttgactt
     7801 ttttactaag agttttatac tatctattat agataataag aatgtgttag aggaggcact
     7861 caccatgaac gtaaaaatct tttctaaaaa taattgtatg caatgtaaga tggtcaaacg
     7921 gtttttaaca gaaaatcaaa ttgcttttga agaagtcaat atcgatgaac agcctgaagc
     7981 agtagactgg ttgaaagcac aaggattcca aagtgttcct gtcattactt ctgatgcaga
     8041 aacagttatc ggattccgtc ctgatcagtt gcgtaaactt gccagctaac atgactaaaa
     8101 gaatcaaaac gtgatacatg gatttgaagg gcttgagaca gcaccagttt ttgtcgcaaa
     8161 cttccaaatc cttttttaaa gcttaaaaac aatgcaatga tttgcaatca agcatcatca
     8221 aatacgaaat gaaggttggg cgtacgtatg agtcttaaaa atttacaaga tgtcagttat
     8281 ttcaagctga ataatgaaat caatcggcca gtcaatggtc aaatcccttt gaataaggat
     8341 aaagaagctt tagttgcctt ttttgaggaa aatgtcaaac caaatacgat gacttttcca
     8401 tctattatgg aaaaaatcaa ttacttgatc gatcaagaat atatagaagc agaattcatt
     8461 caattgtatt caccatcatt tattgaaaag ctatatcaat ttttggacga gcaacatttt
     8521 tctttcaaat cttttatggc tgcttacaaa ttttattctc aatatgcgtt gaaaaccaac
     8581 gacggttccg cttatctgga aacttatgaa gatcgagtag ccttcaatgc gctttacttt
     8641 gcgaacgggg atgaagaatt agcacttgct cttgcagacg aaatgatcca tcaacgatat
     8701 cagccagcta caccttcttt cttgaatgct ggacgtaaac gtcggggcga attggtgtct
     8761 tgtttcttga ctcaagtcac tgacgatatg aactctatcg ggcgctcaat caactctgct
     8821 ttgcaattgt cccgtattgg tggcggcgta gggattactt tatcaaatct tcgtgaagcc
     8881 ggcgctccta tcaaaggata tgagggtgct gcaagtggtg tcgttccagt catgaaatta
     8941 ttcgaagata gtttcagcta ttctaatcaa ttaggtcagc gtcaaggagc tggcgtggtt
     9001 tatttgaacg ttttccatcc agacattatt gctttccttt cagcaaaaaa agaaaatgca
     9061 gatgaaaaga ttcgcgtaaa aactctttct cttggcgtga tcgtacctga taaattttat
     9121 gaattgacac gtaaaaacga agatatgtat ttatttagtc catatagtgt cgaaaaagaa
     9181 tatggtgtcc cattctctta tgtaaatatt acagctgagt atgatcatct agttgcaaat
     9241 ccaaacattc gcaaacaaaa aatcaaagcg cgtgatcttg aaaatgaaat ctcaaaatta
     9301 caacaagaat ctggctatcc ttacgtaatc aatattgata cagcgaaccg cgagaatcca
     9361 atcgatggca agatcatcat gagtaattta tgttcagaga ttttacaagt tcaaacgcct
     9421 tctgtgatta acggcaaaca ggaatatgaa gtattaggaa cagatatctc atgtaactta
     9481 ggctcgacaa atatcgtgaa tttgatgcaa agccctaact ttggtcaatc tgtgcgtacg
     9541 atgactcgtg cattgacatt tattacagat gcatctgata tcgatgtcgt accaccgatc
     9601 caaaacggaa ataagctgaa tcatacgatt ggattaggtg ccatgggctt acacacttat
     9661 cttgcaaaag aacaaatgga atatggctca gaggattcat tagactttac aaatatttac
     9721 ttcatgctgt tgaattattg gacacttgtt gaaagcaaca atatcgctcg tgaaagaaaa
     9781 caagttttcc ataattttga aaaatcagct tatgctgacg gcacttattt cgaaaaatac
     9841 gtaactggag aattccagcc aaaatcagaa aaagtcaaag cattgtttga aggcattttt
     9901 atcccaactg ctgaagattg gaatgctttg aaacaagcag taatgaaaga tggactgtat
     9961 catcaaaatc gcttggcagt agcaccaaat ggctcaattt catatatcaa cgatacgagt
    10021 gcaagtattc atcctatcac tcgcatgatc gaagaacgcc aagagaagaa aatcggtaaa
    10081 atttattacc cagcgcctta tctatccaat gagacgattc cgtattacac ttctgcttat
    10141 gatatggaca tgcgtaaagt aatcgatgtt tatgcaacgg cacaacaaca cgtcgatcaa
    10201 ggcatgagtt tgactttatt catgcgttct gaaatcccag aaggcattta tgaatggaag
    10261 acaacaacga agcaaacgac acgtgatttg aatattttaa gacattacgc tttccacaaa
    10321 ggaatcaaat ccatttatta cgtacgtacc tttactgatg atgcagaaga aatcggaagc
    10381 aatcaatgtg aaagctgcgt gatctaatat catagataga ttcgcttatt catattttaa
    10441 ttaaaggagc attctcatgt cagaaacata ttatgaagca ataaactgga atgaaattga
    10501 agatatcatt gataaatcca cttgggagaa gctgacagag caattttggc tagatacacg
    10561 tatcccactt tcaaatgacc tagatgattg gcgtacattg tctcaattgg aaaaagatac
    10621 agtaggacat gtatttggcg gtttgacact gctagatact gtccaatctg aaagtggcat
    10681 ggatcagttg cgtaaagatg tccgcactcc ccatgaagaa gccgttttga acaatattca
    10741 attcatggaa tccgttcatg ccaaaagtta ttcttctatt tttagtacac tgaatacgaa
    10801 aaaagaaatc gaagaaattt ttgactggac aaatacgaat ccttatctgc aaaaaaaagc
    10861 tgagcgtatc aatgaaatct acaaacacgg tacaccgcta gaaaagaaaa ttgccagcgt
    10921 ctttttagaa acattcttgt tctattcagg tttttatact cctctttatt atttaggaaa
    10981 caacaaattg gctaacgtag cagagatcat caaactaatc atccgagatg aatctgtaca
    11041 tggaacctac atcggttaca aattccaatt aggattcaac gagttacctg aagcagaaca
    11101 agaaacgtta aaagactgga tgtacaatct tctttatgag ctttacgaaa acgaagaacg
    11161 ttatacagaa gaattatacg atccaatcgg ctggacagaa gaagtcaaga cattcctacg
    11221 ctataatgcc aacaaagcat tgatgaacct agggatggat ccactctttc cagatactgc
    11281 caatgatgtc aacccaatcg taatgaacgg gatttcaaca ggcacaagca accatgactt
    11341 cttctcacaa gtcggaaacg gctatttgtt aggtcatgta gaagcgatga aagacgacga
    11401 ttatttgatt ggattggaat aaggatttat tttaattcaa aaatttttcg aaaaataaaa
    11461 agagtttagc gtacagatat tttaaatgta cactgaactc ttttttatgc ttattcactt
    11521 gtttcttcca gcacttttgg actgtctaac ttttccgaaa catgttccat tcttctcaat
    11581 aaatagattt tccggatatt taatattatc gtcttaatca ctgcatagac aggtatgcaa
    11641 atcagcatgc ctaatattcc tgcgacactt cctgctccaa tcaataccaa gatgatcgtc
    11701 aaaggatgga tattcattga tttcccaaat aacaacggct tgatcaaatt tccatctaat
    11761 tgttggatca ctaaaatcga caacgccata tacaaagctt gaaatggaga gataaacaaa
    11821 ccgacaatga ttgctggtac cgctccaata aaagggccga catacgggat aatattcgtg
    11881 attccgcaaa tgaccgcaag caacaagcta taaggctgat cgaatattct cattgccaaa
    11941 aagctcatta caccaataat caaagcatct aagatcgtac tgctgatata ggcagataat
    12001 gtttcgttta attcttttac cgtttggcgt aactctttac gaatcgcttt cggaaagaag
    12061 tgagacaacg catcaataaa tttgtgccca tccttgaaca taaatagcaa aataaatgga
    12121 acggtaaata acaaaataaa aaattgcatc ataaatgaaa tgatttttga gacacttgca
    12181 gataaactga ctaatatgat attgaacaaa ttactaagag ataaatttgc tgatgctaat
    12241 tgttcttcca aattgaaatt tttaaactct tcccgctgcg ccaattcatt taaccatttg
    12301 cttgtttcat ctgcatacat tggcaagctc tgggtcaatt gaattgtctg ttccattaac
    12361 tgaggcacta cattcatgac agttaaaaca actaaaatga ttacgaccaa aaacgtcaat
    12421 aaaaaaccga acactctcgg gatccgatgc ttttcgagaa aaacaacgat tggatcgaac
    12481 atataataga taaaaccagc aacaagcaat ggtaaaaaaa ttgccgaaag cattttcaat
    12541 attggactaa aaatattcgg catttgtaaa ataaagtaca cgccgatgat cgtgagtact
    12601 agctccactg tccagaaaaa taattttgat tgtttaaaac gtgattgcat acatctcctc
    12661 caaggtcctt tgatacttct attctatcat ttaacaaaga gttacgcgct gagattttca
    12721 tttgcttaaa aaaaacaaaa aaaataaaca tattttaatg agtagacgag tacttaaaca
    12781 aaaacacggt ttggagtagt gacaaaaatt atactgcagt ccctttttcc aaataaacgg
    12841 tgttcaaaag cttgcatctt cgtaataagc ccagccaaac gaaaaatata aggaactatt
    12901 tttatttggc tgggcttatt acttgatgta ggacatctat tttacaacct cagatcattt
    12961 attgcgcaat actgactaaa tgcaactttg tgatattttt gtgtacggac aactcctcga
    13021 tcacatcggc ataagtgagc ccttttggaa gatcaatcgt atagatattt ttgtacactc
    13081 ggtagtcatc aacgaatttt acgtcaaaat tcacatcttc gattacgata tttttctctt
    13141 caaaataatc ggtaagaaaa tctttggttt cttttctatg gataaattgg atttctaact
    13201 ttttagtcgt aggtacatgg atcacgcgtt tcactactgt gagtgcaata gcaatcccga
    13261 taaaactgat gatcgcaatg gaatagtaac ccatgccgat ggcgatccct aatccagcaa
    13321 ttgcccataa agaagcggca gtcgtaagtc ccgtcaccga ttgtttcgtg acgatgatcg
    13381 ttcccgcgcc taagaaaccg acaccactca ccacttgagc aatcagcctt gcttcatctg
    13441 aacgaatcgt tccagcaagc tgtggatact ctaaagcatc tcttaatgca ccactagcga
    13501 tctcaacttg gatcaatgca atgatacatg ctcccataca aactaaaata tgcgtacgca
    13561 tacctgctgg acgattttta tattgtcgtt caaaaccgat tgctccgcca aatagcatag
    13621 cgatccctaa acgtaaaata atctctggaa ttgtcaatac catatcattc atctaaacac
    13681 cctccagtcc aatatatcat gtctgaaagg agcagacaaa aaaagtgcaa tttttacctc
    13741 ttacgaaaaa aggactgtga caaaattttt gtcatagtcc tttttatgaa taaacggtgt
    13801 tcaatcagct gaccctcggt attactcaac aaacggcaaa atatggggag ctatttttcc
    13861 tttgtcttct ctaatactca ggtcaaaacg ctgatttcac aacctcttta gcctactatt
    13921 gaaaattaca acttacttgc tgcttcttca tcgatgatca caacaacatt gtcgtgattt
    13981 tgcaatacac ttgctggcaa atgattcgtt acaggtcctt caatcatccc tttgattgca
    14041 tcagctttgc tttcgccaaa tgccatcaag acgatttctt tacttttcat aattgaaccg
    14101 attcccattg aaacagcttt tgttggtaca tcttctgctt tatcgaagaa tcgtttgttt
    14161 gcattgattg ttgattctgt taagtcaact actgaagtcg ttccgtcaaa tgaagcacca
    14221 ggttcattga aaccgatatg tccattttgc ccgattccta agatttggat atcgattgga
    14281 tgtgcatcga tgatttgatc atactttttg catgctgctt ccagatcttc tgcttttcca
    14341 tctggtacaa acgtttcttt gaatggtttt ttatcaaata attggacatc catgaaatga
    14401 cggtagcttt gttcatctga accgcctagt ccaacgtact catctaagtt tacagaagtc
    14461 atttcactga aatccaaatc gcttgaagtc atttctttat ataaagtaac tggtgtgctt
    14521 cctgttgcca atcctaatac ttttgcacct ttgttcatac cttctttgat gatttcaaat
    14581 gctttttttc cgccttcttc agcgtttttt acacgaatga tttccatagt gattgctcct
    14641 ttatattttg gtatagccca attatagcat tctttttttc tttttacaat aattagtata
    14701 gaccaaattg catttttttc attttcttga caataaatag gaatccgtgt taggtttcat
    14761 aagatggttc aatcaaacaa tccatgttgc atgaaaacca agcaagtgat tcactgtgga
    14821 cgctttatgt ctgcagtttt tttatgaaaa aggagttatt atgagtatac tgacattaaa
    14881 caacattacc cagcaatttg gcgacaaaat cctctatgaa ggtgtcgatc tgcaattaaa
    14941 tgctggcgaa catcttgggc tgattggaca aaacggagcc ggtaaaagta cattgattaa
    15001 aatcatcact ggtgagatct taccagatga cggacagatc cattggcaaa aaaatattca
    15061 caaaggttac ctcgatcaat atgtggaagt cgatgaaaca ttaacaatcg aagaatttct
    15121 aaaaactgcg tacgcaaaag aatacgaaaa ggaagaaaaa attgcttttc tttaccaaac
    15181 atatagcgag accttcgatg attccttgct ggaaaaagct gggaaactcc aaacggaatt
    15241 agaccaagga gtgttttatc aaatcgacac cttagttgct gagatgagca gcgggttggg
    15301 gatcgatgtc ttaggtttag attcactgtt gaaaaatcta agtggtggac aacgttcaaa
    15361 ggtgatttta gcaaaactac ttttagaaaa cccagatgtt ctgatcttag atgaaccaac
    15421 caatcactta gatgatcagc atatccaatg gctgacgcag tttcttcagg attttgacgg
    15481 tgcgtatatc gtgatttccc atgaccaaga atttcttgat cagatcacca cccatatcgc
    15541 agacatcgag tttggaaaga ttactaagta tactggccat ctaaaacaag cattgaaaca
    15601 aaaggaacaa aatcgcgaaa gctatttgcg ccaatatcat gctcaacaaa aacaaatcga
    15661 aaagaccgaa gcttatattc gaaaatacaa agccggttcc cgatcaacga tggcaaaaag
    15721 ccgacaaaaa caattagata aaatcgaacg gctgacgcct ccagcttcag caagtaaagc
    15781 tgtctttgat tttccattct cgcctattgt cacgacatta gcagtggaaa caactgattt
    15841 agtcatcgga tatgatcgac cgctacttgc acccatcaat ttgaccatcc gctttggtga
    15901 aaatattgcc attcgaggct tcaacgggat tggaaaatct actttattaa aaacactgat
    15961 cggtgagatt gaaaaaataa gtggcgattt ccacttccca gataacacaa aaatcaatta
    16021 cttttcacaa gatctgtact gggaaaatcc attagaaaca ccactacaat atttaagcag
    16081 tcaatttcca aaagcgacta tcaaagaatt acgtcgccaa ctagctaaag cagggttagt
    16141 taatcaatta gcaagtgaac ctctcactac attaagtggt ggggaacaaa ctaaggtgaa
    16201 acttgcacag ttgacaatga accaaggtaa tttactgatt ctcgatgaac cgacgaacca
    16261 tatcgatcaa gaaacgaaag aaagcttaca agacgggata cagaaatttc caggaacagt
    16321 aatcatcgtc tctcatgaac aagaatttta tcaagatctt gtgcaacgag tcattgagat
    16381 tgaaccaaaa taaacgtttg attgaatagt tgaaaaaaag gagctgtgac aaaagttttg
    16441 tcacaactcc ttcttctgaa taaatggtgg ccaattagct gaccttcggt attagctcaa
    16501 aaaacggcaa aatatgagga gctattttta tttggctatt cttattactt gatgcagaac
    16561 agcttttcac aacctctttt cgtctatttt ttattttcga gatttctctt tcctttaaaa
    16621 acaactgttt gaaagaagaa cgcacatcct tgaaaaacaa gcccgctaac cgttacacct
    16681 aaccagccat actgctgcca cattgtcgtc cctagcaatg aaccagccgc acctccaata
    16741 aagtacataa acataaatac a
//
LOCUS       DAZKOR010000060        16616 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000060 DAZKOR010000000
VERSION     DAZKOR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.060"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      80..246
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            394..951
                     /locus_tag="ACG8ZM_001880"
                     /pseudo
     CDS             394..951
                     /locus_tag="ACG8ZM_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1009..3048
                     /locus_tag="ACG8ZM_001881"
     CDS             1009..3048
                     /locus_tag="ACG8ZM_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0492831.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFINNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNPDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3157..4308)
                     /locus_tag="ACG8ZM_001882"
     CDS             complement(3157..4308)
                     /locus_tag="ACG8ZM_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0492832.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4462..4992)
                     /locus_tag="ACG8ZM_001883"
     CDS             complement(4462..4992)
                     /locus_tag="ACG8ZM_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0492833.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5091..5441)
                     /locus_tag="ACG8ZM_001884"
     CDS             complement(5091..5441)
                     /locus_tag="ACG8ZM_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0492834.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5598..7127)
                     /gene="ahpF"
                     /locus_tag="ACG8ZM_001885"
     CDS             complement(5598..7127)
                     /gene="ahpF"
                     /locus_tag="ACG8ZM_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0492835.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7137..7700)
                     /gene="ahpC"
                     /locus_tag="ACG8ZM_001886"
     CDS             complement(7137..7700)
                     /gene="ahpC"
                     /locus_tag="ACG8ZM_001886"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0492836.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7844..8506)
                     /locus_tag="ACG8ZM_001887"
     CDS             complement(7844..8506)
                     /locus_tag="ACG8ZM_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0492837.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8690..10336
                     /locus_tag="ACG8ZM_001888"
     CDS             8690..10336
                     /locus_tag="ACG8ZM_001888"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0492838.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10327..11409
                     /gene="uxuA"
                     /locus_tag="ACG8ZM_001889"
     CDS             10327..11409
                     /gene="uxuA"
                     /locus_tag="ACG8ZM_001889"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0492839.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11517..13070)
                     /locus_tag="ACG8ZM_001890"
                     /pseudo
     CDS             complement(11517..13070)
                     /locus_tag="ACG8ZM_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            13218..14359
                     /locus_tag="ACG8ZM_001891"
                     /pseudo
     CDS             13218..14359
                     /locus_tag="ACG8ZM_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14485..15564)
                     /locus_tag="ACG8ZM_001892"
     CDS             complement(14485..15564)
                     /locus_tag="ACG8ZM_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0492840.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15667..16107)
                     /locus_tag="ACG8ZM_001893"
     CDS             complement(15667..16107)
                     /locus_tag="ACG8ZM_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0492841.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16164..16574)
                     /locus_tag="ACG8ZM_001894"
     CDS             complement(16164..16574)
                     /locus_tag="ACG8ZM_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0492842.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
BASE COUNT         5679 a         3064 c         3040 g         4833 t
ORIGIN      
        1 taaatttcac tatttcattt caaattccat tgcctaatca ttaggaaaag tctatcatga
       61 actcgtaaat agaatatagt aataaccttg ttaggtgagg ctcctataca aacataggct
      121 gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag aaactgttgg attaaggctt
      181 tttccaatag ctaagaatgt tttctttacg ttgtatagtg ctaaaactcg gacgatgaga
      241 tgatgctagg tcagaattct tgttcgaaaa ggtttctgac cttttcttta tatttttgtg
      301 actgacttat ttgatgagac aaactcgctc ctcacctgac aagtggattg ctgtgtgagg
      361 aggagatgcg gtgaaaaaag atcgattggt ggaggtcgct cggattcact tctggttgca
      421 caatacaacc agaaaacaca acaagcaaaa ctgatctcta tcatgagaga ttcatatgtc
      481 gatattccag gttacggaat gaataagata aatgcagctt actcttatgg gggagttgat
      541 ttattaaatc aaacattaaa ggaaaatttc aaatttgaag ctccctatta tgcaagtatc
      601 acatttcaag attttatcga ttgcgttaat gaactgtttc ctggtggagt caagatcgat
      661 gcagaaaaat ctctagattt agatggcgta tatataaaca aaggagagca agtgatggat
      721 ggtaatactt tactccagta tgctcgattt cgggaagaca aagaaggaga cttcgggagg
      781 atcagaaggc agcagcaagt gatcaaagct atctctcagc agctgaaaga cgtaacttca
      841 atattcaagc tacccaaagc ggttgggaaa ttacttggta gtatccaaac gaaccttcca
      901 gaaagtgtgc ttctggattg tggtatggat ttcctcaaag acatatgtta gaataaacag
      961 gtataaatag tgaaataaag gaatgacaag caagagaagg aggaaaaaat gaaaagaagt
     1021 gacaagcacg gcaaaaatcg aacaggcgct tatattgccg gcgcagtgat cttaatagca
     1081 gctgcgggtg gcggttattt ttactaccag cactatcaag aaacccaagc agtagaagct
     1141 ggagaaaaga cagttgagca atttgtccaa gctttgaaca agggagatta taacaaagct
     1201 gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat tatctgaaaa agagatctta
     1261 gataaatacc agaatatata cggtgctgct gatgtcaaag gacttcagat atcgaatcta
     1321 aaagtagata aaaaagacga ttctacttac agtttttcat ataaagcaaa gatgaatacc
     1381 tcattaggtg aattgaaaga tctttcttat aaaggaacat tagacagaaa tgatggtcaa
     1441 accacgatca actggcaacc caacttggtt tttccagaaa tggaaggaaa tgacaaagta
     1501 agcctgacca cacaagaagc agcaagaggg aatattatag atcgaaatgg tgaaccatta
     1561 gcaacaacag gcaaactaaa acaattagga gtcgttccaa gtaaacttgg agatggaggc
     1621 gaaaaaacag ccaatatcaa agcgattgct tcctccttcg acttaacaga agatgctatc
     1681 aatcaggcga tttcacaaag ctgggtacaa cccgattact ttgtcccatt gaaaatcatt
     1741 gatggagcaa cgccagaact tccagctgga gctaccattc aagaagtaga cggcagatat
     1801 tatcctttgg gtgaagcagc tgctcagctg attggttacg tgggagatat cacagcagaa
     1861 gatattgata agaatccaga attaagcagt aatggaaaaa tcggtcgctc aggtttggag
     1921 atggcttttg ataaggatct tcgtgggaca acaggtggga aattaagcat tacagatgca
     1981 gacggtgtag agaaaaaagt tctgatcgaa catgaagttc aaaatggcaa agatatcaaa
     2041 ttgacaatcg atgcgaaggc acaaaaaata gcttttgaca gtctaggagg aaaagctgca
     2101 tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg cgcttgctag ctctccaagc
     2161 tatgatccaa acaaaatgac aaacgggatc tcacaagaag attacaaagc ttatgaagaa
     2221 aatcctgaac atccattcat cagccgattt gcgacaggtt atgctcctgg atctacgttt
     2281 aaaatgatta cagcagcaat cggtctcgac aacggcacta tcgatccaaa tgaagtgttg
     2341 acgatcaacg ggcttaaatg gcaaaaagac agttcttggg gatcttttaa aacaacgcgt
     2401 gtcagtagtg atgtatcaca agtagactta aaaactgctt tgatctattc cgataatata
     2461 tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat ttcgtacagg cttagataaa
     2521 ttcatttttg gtgaagacct tgatttgcca attagtatga atccagcaca aatttctaat
     2581 gaagatagct ttaactcaga tatcttgcta gctgatactg gatatggaca gggcgaactt
     2641 ctaattactc ctatccagca agcagcaatg tattctgttt ttatcaacaa tggcacactt
     2701 gtctatccta aattgattgc agataaagag acaaaagata agaagaatgt catcagcgaa
     2761 acagcagtac aaacgatcgt gccagatctg agagaagttg tgcaagatgt aaatggtaca
     2821 gcacattctc tttctgcttt agggattccg ttggcagcga aaactggtac agcggaaatc
     2881 aaagaaaaac aggatgtaaa ggggcaagag aacagtttct tgtttgcttt caatcctgat
     2941 aaccaaggtt atatgatggt cagcatgttg gaaaataaag aagatgatga ttcagcaacc
     3001 aaacgagcat cggaactatt gcaatacctc aaccaaaatt atcaataaaa aattgctttt
     3061 taagtacagt aaaaggactg tggcgttatc gtcacagtcc ttttttcaaa taaacggttt
     3121 tcacgatgtc tctttcacaa cttctttttg atttactcac acttcataaa ctttttttgt
     3181 aaaatatttg agaacaagtg gagaaataag tgtacttaat aaaatcacga taactaaagg
     3241 tgaatagtac tgtggttcga tcaattgact ttgctgtcca atttgtaaaa tgatcaaagc
     3301 catttctcca cgcgaaatca taccagcacc gaccatcaat gaactatgtt gagaaaaacc
     3361 ggcaatctga gcaccaaaat aacctccaag caatttggtc aaaatcgcta caagcgtcaa
     3421 tatagaaata aagagtagtt gttcactaaa gtcagaaaaa tcaacttcta atcctacact
     3481 gacaaaaaat actggtataa atactgcata tcctaaagcc tctacattgt ggtatacttc
     3541 ttctttaacc tttgtctgac tgacagctat cccagcaaaa aaagcaccaa tgacagagct
     3601 aagcccaaca agatccgcta cgtaagacat tcctaaacaa ataactagcg atacgatgat
     3661 aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt aggaaaggcg caatccattt
     3721 gactaataag aaaataaaaa caaaataaat tagctgttca atcaaaacaa gcggtaaaga
     3781 agcattgcta gaagcttccc ccgttagaaa agataggctg aaacttagaa ctaaaacgac
     3841 taaaatgtca tctaccacag aagcacccaa aattgttgat ccttcttttg tattcactac
     3901 gtttagttct tttaatactt ctactgaaat actgacagaa gttgccgcaa gaataatccc
     3961 aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg cctgtcaacc agccaaaaac
     4021 aaccgggaag atgatcccaa gcaaagcaac gaacatacca ggtcggaaat attttttcaa
     4081 aagagataaa tcactttcca gaccagctaa aaacattaat aagatcacac caatctccga
     4141 aaagtcatgg actagaatat caggtttgac ccaattcaat ccagcttggc ctagcaaaac
     4201 accaacaagt aattgtccaa tcactgctgg gatacctaat ctccgcgaga tatgtgagcc
     4261 aatcgttgtt gaaatcaata tcaggcataa aataccaata aattccattc tcatcattcc
     4321 tttgtataaa atatagcccg aacttagcat aacatacatt attgaaaaaa taggaaaata
     4381 tactcacaaa aaaaccaaca atccaaaaga tttttttgga ttgttggttt ttatacggtc
     4441 tattttaata actaaaaatg attaacgtat agtcattttt cttattctga tgaaattgat
     4501 aatctcctgg atgcccatga aaatcaaaat aaagccgaat atttggaata gaagcatcaa
     4561 acttttgaat ggattgaaca ataagacgaa tccgccaatc aacaacagaa tactactata
     4621 aacactccaa cttttagctt ttgtattcat gctgaagaac aactggaaca agctgttgat
     4681 gataacagct aatccaagaa gaatcggcag gatagaaacg attgcaggtg caaaagcata
     4741 gacgatcaga gctaagatca ggaaaaaaag accactgaac agaccgatac tccaagaacc
     4801 tgttttcctt ttaaaacggt aagcctcaaa aagattcaac aacccaagca aaacaagata
     4861 ggcggtgatc agataaccga taaaatgaaa aacagcagaa gggtttagca caatcgcaat
     4921 cccagaaata atataaacag cagctcttaa taaggcgtat ttttgaatat tttgtttaat
     4981 cgtctcaaac atataagaaa acctcctttg acttatttta acgtgaacta gtcattttac
     5041 caaattaaat gttacaaaat gagcgaagga gaagaatatc tctggaaaaa tcaacttctg
     5101 aggatctttt ccattttttt gccttttgct agctcatcga tcattttatc taaataacga
     5161 atttctttca tcaagttatc ctcgatgtct tctactcgta caccacaaac aacacctgta
     5221 atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa aaaattgttc gaaagtcact
     5281 tgtttttgta gttgatattc tagttctgtt tgggaataac ccgtcagcca acaaatgatt
     5341 tcatctactt cctcttttat acgatctttt ctctccgctt ttgctacata cataggataa
     5401 acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca taatcagtca cctttctttt
     5461 tttcaaatta tttactactt atagtatata agaaaaagag aagaaaaagc gattattcaa
     5521 aaaaacaaaa aggattgtga caaaattctt gtcaccatcc ttttttccgc tctctgactt
     5581 tttacaatct tcatagctca agtgcgcatc agataatcga atgctcctaa agctgcagta
     5641 gctcctgaac ccattgaaac aatgatttgt ttgtatggac tatctgtaca atcaccagct
     5701 gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg tcactatttc atttcttgga
     5761 ttcatttcga tcgttctatc tagccaatcc gtattaggga gtaagccgac tagaatgaat
     5821 actcctgaaa cagctagtgt atgcatctcc atcgtacggc gatcgatata ggagagagat
     5881 tctacctgat tcgtaccgct gatttctttc gtcgctgcat gtttgataac agtgacattc
     5941 gcaagagaga ctagtttatc ctgtaagatt tgatctgctt ttaattctgg taagaattct
     6001 agaacatata catgttttgc taacccagcc aaatcgattg ctgcttcaac accagaatta
     6061 ccaccaccga taacagcaat ctcttttcct ttaaataaag ggccgtcaca gtgtgggcaa
     6121 taagcaattc ctttattttt aaactctttt tcgcctggta cattgataga acgccagtgc
     6181 gcacctgtcg cgatgatggc ggtttttgct ttaagtgata taccgttagc taagccaata
     6241 ttaataaaat gttgctcttt atttaaggaa acggcctgtt gattcatcat aatgtcgata
     6301 gggtaactac gcacatgact ttctacttgc tgcatcaatt ttggaccttc tgtgtaagag
     6361 gtaccaatca tattttcaat ccctagtgtt tcaacgactt gtccgccgaa agaatcagcg
     6421 acaagtcctg tacggattcc tttacgtgct gcatagatag ctgcactact agctgctggt
     6481 ccaccgccaa tcactaatac atcataaggt tctttttcaa acaactcttc ttcaatcaaa
     6541 gggccagtga ttttttcaac aatttgttcg atcgtcatgc gtccgctact aaattcttct
     6601 ccatttaaaa acacagttgg cactgccatg atttttttat catcgatttc tttttggaac
     6661 atcccacctt caatcatcgt atgagtaata tttggattta atacagctaa tatattcaat
     6721 gcctgtacaa cgtcggggca attatgacaa gtcaagctaa cataactgtc aaaatgcaat
     6781 gtttgtgtta tttttttgat tttaagactc aaagattcac tgattttcgg tgcccgtcca
     6841 ctaatttgta acatagccag taaaaaagag gaaaattcat gaccgagggg gataccagca
     6901 aaaacgattc cactttcttt gccaactgga ttgatagaaa aactaggtgt gcgcatcgaa
     6961 tctctttttt cccaagagat tttaggagac atttctgcta tttccttcgt aaattcagct
     7021 agtttttgac tattttcact attatccaaa ctaacagaaa aaacaatagg agattcaatc
     7081 agtgctaaat attgttcgag attttgttta gtttctttgt ctaacatatc caccacctaa
     7141 atctttccaa caagatcaaa acttggtttc aatgtttcgc cactttcttt ccattttgcc
     7201 ggacaaactt cacctgggtg ggtacgtaca tattgagcag cgcgaatctt atcaatcagt
     7261 gcactagcgt cacgaccgat tccatctgca ttgatttcca tagcttgaac gattccgtct
     7321 ggatcaataa taaatgttcc tcgctgagct aatccttgct cttcatccaa aacatcaaaa
     7381 atacgagaaa tctgatgaga cggatcaccg atcatcacgt attcgatttt accgatagca
     7441 tctgatgtat catgccatgc tttgtgagta aagtgggtat cggtagagca agaaaagact
     7501 tcaacaccta aagatttcaa tgtactgtat tgttcttgta agtcttctaa ttcagtagga
     7561 caaacaaaag taaaatcagc tggatagaag caaagaatac tccagttccc cataagatct
     7621 ttttcactga cttctaaaaa ttctccttga tgataagcat gtgcattaaa ttcttcgatt
     7681 tttttcccaa tcaatgacat agtaaaacct ccaataataa ttatttattt ataattattg
     7741 taatttagtt ggagttgcaa agtcaatgga tattttgtta tttaataaag attactcaaa
     7801 aacgaaggtt aattataaaa atagaagagg tcgcaagaaa atgctactgc tggaaatact
     7861 cgggataacg ttgagtaaca gtttcctgtt ctttcagcat catgtgtaag tgcaatcccg
     7921 tttgaaatcg agcttcttct ggttttttat caactaaagc cttgagtaaa tcataatgtt
     7981 gttccatgat cgtttcccat ggaagcccct gaatggtcac tcgtaaccag cggaaacgtt
     8041 ctaaatgggt attatggtca tctaaccagt tccaaacttc ttttcttcct gcaatttcga
     8101 aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc gcgttcgtca tttattttga
     8161 ctgctttttc ttgttcttct aaaatcgttt ctagcactat tcgtgtttgt gtgtccaatt
     8221 tggaacaaca ttcaatcatg atttgctctt ccaactgttc tcggacaaag cgagcatttt
     8281 ctgcagaaac aagattaatt ttggaaacat acgtaccact ttgaggtatc gtatacacga
     8341 gttcttgctg acgtagttga atcaatgctt cgcgaatggg agtccggccg atagcaagat
     8401 gttcctccag tgccttttca gatattttcc ttccaggttc taagtcagca tagataattt
     8461 gtttacgaat cgcttgataa gcttgtgatt gcaagctgct tttcatcttt attcctccaa
     8521 aatcatttct atgaacgtag tataacagaa gaggtctgaa aaacaatctg gtatgtcaca
     8581 ttaactagta aatttgtata aatagtaaaa ttttggttga catagcgatt tttattgcat
     8641 aaaattcact tgaatacaaa aactaatatg ttagtttagg aggggaaaaa tgattgattt
     8701 aaacaactgg aggaacttta tccctgaatt atctcaaaaa gaagtagaaa tacctcattt
     8761 tgaccgtgca tcattaagag aaaatggaca aaaaaatcct gtctggatcc atttcggtgg
     8821 cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa cgtttagcgg atctaggcga
     8881 attgtccact ggtgtcattg tttgtgaaac attcgatgaa gaaataattg aaagggctta
     8941 ccaagatttt gatgatttga ttcttgaagt ggtcatgcca agcagtggag agttcgtcaa
     9001 acgtatatta gctgcgacag ctgacagtct gtattgtcat ccagattgtt ctgaacactt
     9061 tgataagatg attcgacact ttgaaaatcc tagtttacaa ttagttactt ttgctattac
     9121 tgaaaaaggt tatcgattga aagatacaaa taataaatat ataggaccga ttgcagtcga
     9181 tataaaaaat ggtccaaaga agccatcaca tacaatgagt attcttgctg tgctacttta
     9241 taaacgtttt ttatctggag cttggccgat tgcacttgtc tctacagata atttttcaca
     9301 aaacggtagt caattacgta cttctttgct aacgatcgca aaagaatggc aaaaggaagg
     9361 ttatgtacaa aatacattta tccaatactt atcgaatgaa gaaattgttg cgtttccatg
     9421 gacgatgatt gatcgtatca ccccgaatcc ttccttgtca gttagcaaaa tgttggcaaa
     9481 agagggcttt tcaaatatgt cgattttcca aaccgcaaaa ggaaataata ttgctccatt
     9541 tgtcaataca gaggacatcc attatttaat catggaagat cattttccca atggacgtcc
     9601 gaaattcgaa aaagctgggg tattcttgac tgatcgtgca acggtagata aagcagatac
     9661 gatgaaggtg acgacttgcc ttaatccttt gcatacgtct ttagctatta tgggttgttt
     9721 actggggtat aggagcatag ctgaggagat gaaagatcca gatttatcgc tgctagtttc
     9781 tgaaatcggg tacaatgaag gcctcccagt tgtggagtct ccaggtatca ttgatccaaa
     9841 gatctttctg gaagaagtaa ttactgtgcg cttttctaat ccaaatattc ctaatatgcc
     9901 tcagcgaatc gccacggata cctctcaaaa ggtggcgatt cgttatggtg agacgattaa
     9961 aaaatatatg gaaatagaag ttaaagaccc gcagacactt gtattcatac cattgactct
    10021 cgctgcttgg cttcgatacc ttattggcat tgatgatcaa ggaaaaccat tcatcccgag
    10081 tccagaccca ctattgacag accttcagca taagctttcc gatattcaac tgggtacgca
    10141 agatacatca catattcatg agaaaatggc tcctattttg tcaaataaag aaatttttgg
    10201 tgtagatctt taccaagcag agcttggcag aaaaatagaa gaaatgttca tcaagatggt
    10261 agcaggacca aatgccgtac gaaaaacatt acaggattac ttacaagaac atggaggaaa
    10321 tttatcatgg aaatgacttt tagatggtac ggatcacagg aagaaaagat taccttagat
    10381 gaaatcagac agattcctgc ggtcaaaggg attgtaggca cattgatgga tataccagta
    10441 ggagaggtat ggcctaggga gaggatcaaa gaactaaaag aagaaattga gtctcgtggg
    10501 ttaacactaa aagtaattga aagcgtcaat gtgcatgatg ccattaaaat tggattacct
    10561 tcccgtgagc agtatatcga aaactataag cagacaatcc gaaacttggc agaatatgga
    10621 atagaagtca tctgctataa ttttatgcct gtatttgact gggtgaaatc tgaccttgac
    10681 taccgcttag aagacggctc atccacgtta gcttttatat ctgatgatat tccagatgat
    10741 ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg aatttggatt gccgggatgg
    10801 gaacctgagc gcttagctcc tatcaaatcc ttgcttgaag cctatgcatc agtagacgaa
    10861 gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa tcatcccaac atgtgaagag
    10921 gtaggggtca aaatggctgt acatccagat gatccgccat actcgatttt tggattagct
    10981 cgaatcgtaa agaatcgaga agatttagat tggttgtgtc gcatagtaga cagcccttat
    11041 aacggaatca ctctttgtac agggagtatt gcagaagatc cagaaaatga tgtatatggg
    11101 attttagcag aattttgcca aagagatcga atcccatttg cacatgttcg aaatattaaa
    11161 tttttagcta atcgagattt ctatgaggca ccacataaat ccgaatacgg ttcattagac
    11221 atgtaccgca tactaaaaac aatgtatgac aacggattta acggatacat ccgtcctgat
    11281 catggaagaa tgatctggaa tgagacagga agaccaggat acggcttata tgatcgagca
    11341 ttaggtgctg tttatctaaa tggattatgg gaagcaatcg aaaaaggaaa caaaagtaaa
    11401 gataattagt gaaaaaaact gtaattttat tatgtaaata agaaggatgt tcaaattctt
    11461 gatacataaa tccggaggtt gttaataagc attcggattt atgtatctta tttggtctag
    11521 gaagtaactt gattttcttt tttcgattga tccatgagtg cttcttcttt taattcaaag
    11581 atatattctg agatcctcat aatcactttt ttccagtctt ctggtgtgat gattcctttt
    11641 aaaatataga tttttttgat atgagaaggc aattgtttca aagggcagtc gctgataacg
    11701 atgttgcagt tctccataaa atcattttgt attttgatcg attcatttga aaaaatttgt
    11761 tctatcttaa cgatcagatc tttactgata taaaaatcac gcgtatagtt cacaccaatc
    11821 acaattcgcg gctgtttttt gtagcgatca tacaaatgta ctaggtcttc aaccatccat
    11881 tgcacatttt ccttttctaa aataactggt aacaagtgtt gattttgttc tctgaaatcc
    11941 atataaaatt gttcaatggc ggtttggact tctttcaata cgctatgttc ttgataaagc
    12001 atgaaagtag gaaggggcgt atttggttga ttgaagttaa gaaatccatt ataaagttta
    12061 tttcgtgcga atgccaatga gtaaagaatc ttttcttttt caggtataaa caaatcaaat
    12121 acagtacaaa gggactccac catttctctt gaataaagaa catgaggaac gtttgctttt
    12181 tttaaaagtt tgtattgctc caagcttgtt tcttccgatt cgattttcgt gatagaaagt
    12241 cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa ttggcagatt aggtcgcaaa
    12301 atatctacag agagatcaat aaaaaagtca atatattcat actctgtgaa ttctttttgt
    12361 acgtctttca cagaagttgc aggaaaattc tctttacata tttttaatag aaaatataat
    12421 ttatctatca taccgctatt tagtcggtca agtacttctt ttttaaaaaa agtattcatc
    12481 ataaaagcca ctacttgcgg atcttcatca taaaattctg tagcagtact tgaaaagatg
    12541 ttccaataaa tatctaatgc tgcatattga atttgtacct cttttccaga aaaagacttt
    12601 ctgccattat tagaaaagga gatagacaaa ccgtactatg ctaaaaatgc attaatcttt
    12661 ttcattttcg tatacaaata agaggcacta tagtttgttt gctgactgaa actgataatc
    12721 gaatttgtct gacagctgaa caaagaagtc aagatctgat aactgactga ttcgttgcag
    12781 tatcttccaa atagacgata atagcagtaa gccggatcaa ttccttcaaa attcaacttt
    12841 aaaaaaggtt tttgcttatc aatatgcagt tctgttagat tgagacgctc aaagtcttgt
    12901 tctaattctt tgatgtatag taaaaaattt gattcagaca tatggaaatg atctgctaaa
    12961 tattgtgtag taataaagaa acgatgttca gataggaaaa gaaaaatatc taattttgta
    13021 ttgctttttt tcttcaagat aaacgtttta atcgcttctt gagtttgcat gaaagaggct
    13081 cctttttact taaattgtta aaacttgaaa atattactgt ttttttcata aatttagaca
    13141 aaaaaacagt aaacatatag gtagaatctc tactatctta tttcttgcat acaaaaaaat
    13201 caaggagtga gagagttatg agatattcaa ccatcttaat atcaacattt gcagccttaa
    13261 gcttatcgct tactataaat cagacagtcc acacagagga gacagaaaca gccaaatgga
    13321 cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca ggatgaattt tcatccttca
    13381 attcaggagt cgcaaaatta agaaaaggac aaacagcgac catcaaagca aaaattgact
    13441 attcgggaga cttagcacct atactacaat cagctgttac atttgagaat gttgatccca
    13501 ctttgtcgat tggctatgac gagaacactt tgactatcca acacaagcaa gcgatattta
    13561 ccttcaccgt tacattaaat caggacttgt taaaaccagc gctttttact gtcaaggtat
    13621 cagatggact accgaacgat catcatttaa cgccgtatag tcagcggcag acaattgagc
    13681 aagattcagc catcgactca ggaggagatc ttgttgaaga accaacagat aaaccagaaa
    13741 atgaaaataa gccagaagta ccgcctacag aaaatccaga cggagaacag aaaccggaaa
    13801 tagaacctgg agaagaaccc gatacagaaa ctcaaccaga gccagacaat aaatcaaaac
    13861 ctgaaataac ccctggagaa aaaccagatg taactccaga accagatacg gattctggaa
    13921 accaaacagt accggaaaca aatccagaca cagataatga aactgaaaat ccagaaaaac
    13981 cagaggtaga tccagaagaa aaaccagatg tgactccaga accagatacg gacgcaagag
    14041 accaaggaat accagaaaaa ataaacaaaa aaacaattca agaggatgga aaaaaagaga
    14101 gtaaaaaatc aaatctagca attctaaaaa taaatgaaga gcagctgaat aaaaaatcac
    14161 gaatatttga tagcgcccaa tcagctgaaa ccttaaaaag tagcaaggat acaacctttg
    14221 ctgtcctgaa acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat
    14281 actgtggagc ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa
    14341 acaaaacaga tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag
    14401 aaatatccag aaaatgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat
    14461 actttatctt tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg
    14521 gatctttttc actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa
    14581 gccccaaacg ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg
    14641 cagttctgtt ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac
    14701 aatcaaaatc gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat
    14761 gaaagataga ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa
    14821 aatcatgatc gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt
    14881 gagcaacgta tttaaaagag cgacaacaaa ttgagcttct aataccaaac caaatgtatt
    14941 gacaaactta tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa
    15001 aaacaatctt gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat
    15061 gaatgacata gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat
    15121 cgaagcgcca ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata
    15181 ttgtaaaaca ggatctgcat cttttggctg atgctcgtaa aaattaataa cggactggat
    15241 catgtcaaat gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag
    15301 gtaaacgatc agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg
    15361 ctgaatgatt tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt
    15421 tgtaatcata cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg
    15481 gcgtattttt tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata
    15541 acaaaaaata aacacgttat tcattatatg atggaatatg aaaataagaa agagcaaaaa
    15601 gaaaaaccgc taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta
    15661 gcagtttcag ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg
    15721 ttgcagtgta tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat
    15781 accttctgct tgtttttgca acgcagcacc ttgagcagtc agcgtaacat ttacgattct
    15841 ttcatcaccc gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa
    15901 gggagtcagc gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt
    15961 tttcttttcc cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt
    16021 gaggagggga gtgtagtgtc taatgatttc tttagaggcg gcatataaag gaaaacatac
    16081 ttgattaact aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc
    16141 atagcggatt tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt
    16201 ccagctgtag cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt
    16261 tcttccagag aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt
    16321 tcactttctg aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt
    16381 tgctgtttga tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat
    16441 tgttcagggt tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat
    16501 gtacgatcgg gattatgcac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa
    16561 tacatttttt tcataaaatt accaccttta attatatttt atacaattga atttta
//
LOCUS       DAZKOR010000061        16179 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000061 DAZKOR010000000
VERSION     DAZKOR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.061"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            201..1319
                     /locus_tag="ACG8ZM_001895"
     CDS             201..1319
                     /locus_tag="ACG8ZM_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492843.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1822..2142
                     /locus_tag="ACG8ZM_001896"
     CDS             1822..2142
                     /locus_tag="ACG8ZM_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492844.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            2595..2795
                     /locus_tag="ACG8ZM_001897"
     CDS             2595..2795
                     /locus_tag="ACG8ZM_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0492845.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            2942..5731
                     /locus_tag="ACG8ZM_001898"
     CDS             2942..5731
                     /locus_tag="ACG8ZM_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0492846.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            5778..6305
                     /locus_tag="ACG8ZM_001899"
     CDS             5778..6305
                     /locus_tag="ACG8ZM_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0492847.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            6326..7516
                     /locus_tag="ACG8ZM_001900"
     CDS             6326..7516
                     /locus_tag="ACG8ZM_001900"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0492848.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            7556..8854
                     /gene="asnS"
                     /locus_tag="ACG8ZM_001901"
     CDS             7556..8854
                     /gene="asnS"
                     /locus_tag="ACG8ZM_001901"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0492849.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            10030..10704
                     /locus_tag="ACG8ZM_001902"
     CDS             10030..10704
                     /locus_tag="ACG8ZM_001902"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0492850.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            10701..11042
                     /locus_tag="ACG8ZM_001903"
     CDS             10701..11042
                     /locus_tag="ACG8ZM_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492851.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            11072..11431
                     /locus_tag="ACG8ZM_001904"
     CDS             11072..11431
                     /locus_tag="ACG8ZM_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0492852.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            11956..14040
                     /locus_tag="ACG8ZM_001905"
     CDS             11956..14040
                     /locus_tag="ACG8ZM_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0492853.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            14331..15797
                     /locus_tag="ACG8ZM_001906"
     CDS             14331..15797
                     /locus_tag="ACG8ZM_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0492854.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         5372 a         2776 c         3466 g         4565 t
ORIGIN      
        1 tatcagcatt tcatgaagaa tagttgaaga ctggggctaa ataattgagg aaattatgaa
       61 gataacttaa aaaaatagct aaatttaaaa ccgtagaaac cgttttattt ttttagatac
      121 gttttattgt atggaaaaat agtgttgtgg tagactttag atagaaacaa aggaatgatt
      181 tttttaggag gggtacgttt atggcaaaaa aggataaaga attattgtca caattgactt
      241 cagaaggcgc taaaggacca ttcgtcacga tcatgctgaa tacgcatgtc gcccatcaag
      301 atgtggaaaa agatcagatc aaattcaaaa attttgcgaa agaagcaaag aaacgttttg
      361 agaaaaaata ttccgaacat gactggtccc cgttccaaga aaaaattgaa aatttgttag
      421 gagatccttc gttttggcgt agtgcaacta cgagtgtagc ggtcattata actgctgacc
      481 aaacctatat ccatcgtttg agtatcccgg tagacgatca atactatgta agtgatcttc
      541 cttatttact ggctattatc aaaaatgggc agttcaacta ttcttattat ttattgggat
      601 tgaaccgcga ctccatgaaa ttgtatgaag tcagcaacaa acaagtaaaa gaagtatctc
      661 taccagaagg ggcaccggtt gatgtcgtta ctgcattggg atctgaactg acaggtggcg
      721 gtttgaactt ctcttctaat ggaggaggcg gagcccgaga tggagtaact ttccatggcg
      781 taaatccaaa agatgaagaa gtagagatcg attgggtcaa ctattaccaa gcagttgata
      841 cgtacttgaa ggattctttc gaaaatccag aacaatggcc gatttattta tttgctctac
      901 cagaaaatca gacgatgttc aaaaaaattg ctaaaaatcc ctattattgt gcagacgttg
      961 cgatttctgc ttctcctgcg cagctatcct ttagagatat cgaagaaggg gcgaagaagc
     1021 tgtcaagtga actcgcagct caagaatcaa aagaatacaa taaactctta gataagaaat
     1081 tcatcgatca gttagttgat atcgttccag ctgcgaaaga aggaaaagtg tcacatttat
     1141 ttatcgctac gtcaaactta gctgatggtt tcggtgaaga tcctgaaaca gaatatgatc
     1201 ggcgtcaagt gttgaatgca ttagcagatg atgtgatcat taatggcgga aaagtgttta
     1261 ttttggatca aaaagatgcg ccagatgaaa agagtctgac agcgattctt cgttattaaa
     1321 agaggttgtg aaatcagtgt tttgacctga gtattagaga aacaaacgga aaaatagttt
     1381 cacatatttt gccgtttttt gagctaatac cgaaggtcag ctgattaaac accgtgtatg
     1441 agaggttgtg aaaaagctgt tctgcatcaa gtaataagaa cagcaagcaa aaatagctct
     1501 tcatattttt tgtttggtgg gcttattacc gcagatgcaa gcttttgaac accgtttaag
     1561 gaagaggttg tgacagaagt ggtcagcttc aagaaataag aagagattga caaaaattat
     1621 ttctcaaatt tttgtgaaat ttcggcttat ttccgaagaa gctacttctg gaacaccgtt
     1681 taaaaaagag gttgtgacgt ggtcacgacc tctttttgac gattatgata caaaaaacat
     1741 catttatgaa caaaatgtga ataacgggcg ttcttgagat atgattttaa ttgggaaaaa
     1801 cttatcgata aggaggtctt tatgaaaaaa agactgttat ggtgtttgct gtctctatcc
     1861 ctaagtttaa caagtcagcc agtttttgct gaagaaacac agcggtttcc tattacaggc
     1921 acacgtctgt acaaaaatgt ttatgtacag acaccagatc ttgattctgc gattcctgaa
     1981 acagaagaac ggtctttgca gccaagattt ggttactttt ggtactgtcc gacctgtgat
     2041 tatgaaagtt gctggcattt cttacattct acagctgcag cttttgcttt tactcatact
     2101 cagaaatatg atggacatca tcctgttatt tatgaggtat agatatgagc aaaaaagaaa
     2161 aactaatctc gttggtcgta ttggtttgcc ttgttcttct ggtaactgtc ataggctatc
     2221 agaaatacca tgagaacatc agacctacta aagaatcttc cccagcctcg accgttgaaa
     2281 cagaaccgat aagtagtcag aaagaataat ttttttagtt taaaaaagaa catcagagca
     2341 aaaaaacacc aactgaacaa tatgtcgttt attttaaaaa aaagttcggt ttttcacttt
     2401 atgtaatcgc tgagaaaact ttttttaata aaaaaagtcg aaaaactcta gcttttttgt
     2461 aaacggtatg atatactata ggagtagttt ttgttcggta atctgtggta gcttgtttca
     2521 aataaacatc ttccagatat acaccattca agtaattgca aaatatgtgt tgatacacaa
     2581 ggaggatttt tattatggaa acaggtacag taaaatggtt taactcagaa aaaggatttg
     2641 gattcatcac tgcagaaaac ggaaacgatg tattcgttca tttctcagct atccaaggcg
     2701 acggattcaa aactttagaa gaaggccaag cagtaacttt cgatgttgaa gaaggccaac
     2761 gcggacctca agcaacaaac gttactaaag cttaattaac ctatactaaa aaataaacgc
     2821 ccagacattt gcgtgtctgg gcgtttttgt ctattttctt taaaaagcga tgagagtatt
     2881 tttctgcact ttgtagtata atttcaaaag aacattagag gcaggagaag aggaagatat
     2941 tttgaaacca agaacttatg cagtcgttga tatcgaaacg actgggacga atccaaaaga
     3001 agatcggatc atccaatttg gctgcgtcat gattgaatca ggtcggatca cttcacgttt
     3061 ttcgatcgat attcatccag ggcggaagat ttcaaaacaa atccagcatt taacagggat
     3121 cacgaatcaa cgcgtccaaa aagcgcctta tttcgaagat gtcgcccaaa cgatttacaa
     3181 tcttttggca gacaccatat ttgtcgcgca taatatttat tttgattata attttttgaa
     3241 ccatgaactc atgcgatgcg gacttcctag tttgaagata ccaggcatcg atacagtaga
     3301 acttgcacaa attttcttgc cgacggaacc aagctttcgc ttagctgatt tgtcagaaag
     3361 ccttggattt attcatgaga atcctcatca ggcagatagt gatgccgaag tgacaggtca
     3421 acttcttttg ctgatcgaag aacggatgag aaaattgcca atcatcacgt tagagcagat
     3481 tgcgcgatta agtagacaca caggtatgga tacgagtcgt ttcatttatc atgtgattga
     3541 agaaatgaaa gaaaaaccag agccacttga tccctcatta gaaatcgtcg atgggctagc
     3601 tttgcagaaa aaagaagtgg aactattcac atccgtccat tatggcgagc ggacatatcc
     3661 aagaaaaaaa caagcaaaag aaaaaatgtt tggtaaaaca ctgatgtatc gaaaagaaca
     3721 gaacagactg atgaatgcgg tctacgacca tttcacaaaa gatgaatcaa aagatttgat
     3781 gatcgaagca gctacaggaa tgggtaaaac aatcggttat ctgctgccat tgagttattt
     3841 ggctacacct gaaaaacccg ctgtcatcag taccgtttcg ctagttttgc agcagcagat
     3901 catagaaaaa gacattccat tgctgaatca attgctagat cagcccatac aacctgtaat
     3961 tatcaaaagc tatcgccact atattgattt gcagcgtttt aaaggcacac ttgtggaacc
     4021 gccagaacaa aaacaatatg ctttgtatca aatggcggtc ttagtttggc tgacgcaaac
     4081 gaagactggt gatttggatg aattacattt aacaaatttg aaccattcat tctttgcaga
     4141 tatcgcgcac agaggtattg gatttttagc aagaaatcag tcattttacg aacaagactt
     4201 tgttcgtttc ttgcaaaaga aaatcaggca aagtaatttt ttgattgtca atcatgcgtt
     4261 tttagtacaa gaaacacagc gaaaagaacc attgcttcca gctagtccat ttttgttgat
     4321 cgatgaagta catcatttgc cggaaatcgc tgaaaaagta aatgacaaac gattaagtat
     4381 taactatttc cataaacaaa tccagcaatt agaagaacca aatcagctgt ttgaccgaat
     4441 acaaagctat ttaccgaaag aacatgaagc cttacgctgg ttatcgattt atcgagaaga
     4501 actttctgcg ctatgcgaag cacagatttg tatggcagaa ggattaatga gtttagtgag
     4561 cggcaagcaa cctaacggac agtatccaga agaagtcatg atgacaaaag agatgttgga
     4621 tcagctgcca ctggaatctg ggacagccat tgatcacctg cagctttttt atcaggagat
     4681 ccagcagcta cagtcacaaa tgcgtctgac ttttgagatt atccaagaaa gctggacaaa
     4741 ccagatgcgg atcgatttag caagtttata tagcctattt gatcaattag acaagcaata
     4801 cttgctgatg gatcaatggc tggaagactg gcgagatcat ttgatccatt ggttcgttcc
     4861 tgggaacaca gcgcagcaga tgatttttca tctccatgat tttcaagcgg ctatgttgcc
     4921 gtcgactgtt tggtatcctc gatatgatcg tattctttat attggaggaa cgctgaagat
     4981 cggccctaat cgacactatc tctctaagag gcttggcatt gaagcagcac ctcttaaagt
     5041 gattgcctct ccttatgatt atgagaaaca ggcaaggatc tatgtgccaa aagaggcacc
     5101 tgccgtccat caagtcagca atcaagcata tatcgattat ttagagacag cacttacagg
     5161 tctgatcgaa caagaagatc ggccaatact tgtgttattc acttctcatg atgttttgca
     5221 gaaggtctat cagaaaatgc atctgtctat gctagaaaaa ggacgagaaa tactagctca
     5281 agggtttggt ggaagccggg agaagctttt gaaacgcttc attttgtcag aaaaaagcgt
     5341 attgtttggc gctgacagct tttgggaagg tgtagatttg cctggagatg ctttgcagat
     5401 cgttgtagtc acacgtttac ccttcgataa tccgcttcgc ccgatggtca aagctaggaa
     5461 cgcttattta gaatcagagg ggatcaatcc tttttaccag gaatccgtac ctaaagcagc
     5521 tttgaagtta cgccaagcat taggtcgctt gataaggacg gagacagatc gtggcgtatt
     5581 gcttattttg gatcgaagac tagtcactgc aaaatatggc gcacggatca tccatgcatt
     5641 accgaaaaaa gtaccgatca aagagctagc tatggaggaa atctatcagg atatccatga
     5701 attcttaaag aaaaatgaag aaggaagtta gaggagctgt cttttttcat aattaaaatc
     5761 tgctataatg aaaaggaatg aagataaagg agcgacgacc cttgaagaaa aaacagacaa
     5821 aggaaaaaac gattaatcgc gtgttacttg gcttgatcgt atttttatta gcggtcatcg
     5881 tgatcagctc gattttttac ttgcgttcca gtcgtccggc ggtgcaggcg aagaaagaag
     5941 cagtagaaat cgcaaagaaa tatgcaaatc ttgaggaaac cgatcatttt tattggttta
     6001 ctcgaaagaa gacatacttt tcactgacag gtacaaatga taaaggaaaa gaaatcgccg
     6061 ttatcattcc taaatctggt gaaaaagtaa aagtattgga tcaaacaaag ggactgactg
     6121 aaaacgaagc aaaacagaca attgctgcgg cccatcctga aatccaaatc gaaaaagctg
     6181 ctttagggat gtatgatgat cagcctgtgt gggaagtagt aggaaaagaa acaaatggga
     6241 aactgaatta ttacctgatt ggctttgaat ctggagatga agtcaagaca atcaagggaa
     6301 tttaaaaggt aaaggaatga atgggatgga gttatcaaaa agaacaaaac gattagaacc
     6361 gtctgtcact ttggcagctg cggcaaaggc caaggcatta aaagcaaaag gcagagatgt
     6421 gctgagtttg actgtgggag aaccggattt tgcgacacca gaaaacatcc aagaagcagc
     6481 aatcgaagca atccgcaatg ggaaagctag ctattataca ccaacagcag gaatcccaga
     6541 gcttcgacag gcaatcgttg attatctgaa gaaaaactat ggacttgtct atgaaccttc
     6601 acaaacagtc gtaacagacg gagcgaaatt tgctttgtat acccttttcc aaacgatcct
     6661 tgacccgcaa gatgaagtca tcattcctgt gccttactgg gtcagttacg gtgagcaagt
     6721 aaaactggca gaaggacttc ctgtctttgt gaaaggggaa gaatccaata gctggaaagt
     6781 tactgttgaa cagctagaac aagcacgttc agaaaaaaca aaagccgtaa tcatcaattc
     6841 tccatcgaat ccgactggaa tgatttatag caagaatgaa ctgcaggcaa tcggtgaatg
     6901 ggcagtaaaa aatgatattt tgatagttgc tgatgatatt tatggccgtt tagtttataa
     6961 tggcaatgag tttactccaa ttgcaacgat ttcagaagca atcaaaaatc agaccatcat
     7021 catcaacggc gtgtctaaaa catatgcgat gacaggctgg cgcattggtt atgcggtggg
     7081 aaatcctgtg atcatcaatg gaatgatcgc tgttgcttcc caatcaacta gcaatccgac
     7141 agcagtcagt caatatgcag ctgttgaagc attgaaaggt gagcaagata cagtagaaga
     7201 aatgcggaac gcctttgaag aacgcctgaa tacgttatat ccgcttgtgg cagaactgcc
     7261 gggtgtttct cttgaaaagc cgcaaggagc tttctatctt tttcctaatg taaaagaaac
     7321 gctacgcatg tgtaagtatg aaaatgtcac acaatgggta gaagatcttt tagaagaaac
     7381 aggggtagcg cttgtgactg gcgaaggctt tggggcacca gaaaatgtcc gcatgagtta
     7441 tgccactgat cgcgctacgt tagaagaagc ggtacgcaga atcaagcaat ttattgagag
     7501 taaaagtcaa aattaatcct gaagaaattc aaggaatata aaaggagagt catacgtgga
     7561 acaaattcaa atcatcgatt cgaaaaacca tgtgggagaa acagtaacaa tcggcgcttg
     7621 ggtcgcaaat aagcgatcaa gcggaaaaat cgcattttta caattaagag acggaacagc
     7681 ctttttccaa ggtgtagttg taaaaagcga agtgtcggaa gaagttttcc aaacagctaa
     7741 aaatctacac caagaaacat ccgtgttagt aactggagaa atccgtgaag acagtcgttc
     7801 aaaatttggt tatgaaatcg gaattacaaa tgtggaagtg gtaagcgaga gccacgatta
     7861 tccgatcaca ccaaaagaac acggaacaga tttcttgatg gatcatcgtc atttatggct
     7921 gcgttcttca agacaacatg cgattatgca gatcagaaat gaaatcattc gtgcaacgta
     7981 tgaattcttt aatcaacatc attttgtcaa aatcgatccg ccgatcttga ctggttctgc
     8041 accagaaggc actacggatc tattcgaaac aaattacttt gatcaaaaag cttatttatc
     8101 tcaaagtggc caattataca tggaagctgc tgctatggct ttcggaaaag tcttttcatt
     8161 cggtccgact ttccgtgctg aaaaatcaaa aacacgacgt catttgatcg aattctggat
     8221 gatcgaacca gaaatggctt tcactcatca agaagaaagt ttagaagtac aggaacaata
     8281 tgtagcattc ttagtacaaa atgttttaga tcattgcgaa tatgcattag atgtactagg
     8341 acgagacaaa gaagtcttaa aaacttatac aaaattgcca ttccctcgta ttacttacga
     8401 tgatgcaatc gaattactga agaaaaatgg atttgatgac atcgaatggg gcgaagactt
     8461 tggttcacct catgaaacgt tcattgcgaa ttcattcgaa cagcctgtct ttatcctaaa
     8521 ctatccaaaa gccatcaagc cattctacat gaaaccacat ccaacaagag aagacgtggt
     8581 aatttgtgca gatatgatcg caccagaagg ttacggtgaa attattggcg gaagcgaacg
     8641 ggcaacagac tatgatttct tgctagaaca aatccgtaat cacggtttgg atgaaaaaga
     8701 atattcatgg tatcttgatc tacgtaaata cggttctgtt ccgcactctg gttttggtct
     8761 aggccttgaa cgaacagtca cttggattgc tggtatcgac catgtacgtg aagctattcc
     8821 attcccacgt ttgttgaacc gaatttatcc ataagaggtg atgaaaaagc tgttctgcat
     8881 caagtaatta gaacagccaa gcaaaaacag ctgttcatgt ttttgaattg gcgggactta
     8941 ttaccgcaga tgtaagcttt tgaataccgt ttagtggaga ggttgtgaaa aagctgttta
     9001 gtttcgagta ttaagaaaaa ttttgtggaa atggctttcc acatttttgc ataaatttgg
     9061 cttaataccg agaaactggc ttttgaacac cgtttaagga agaggttgtg aaatcagtgc
     9121 cttgacctga gtattagaga aacaaaacgg aaaaatagct tctcatattt taccgttttt
     9181 ttagctaata tcgagggtca gctgattgaa caccgtttat atcaagaggt tgtgcgcctg
     9241 ccgttcagct ccgagtcaca aggagtaatt tcaaaaaaca gtttttcatg tttgatgact
     9301 attgtgactt gtgaccgagg agttggccgg agagcaacgt ttatttaaaa aaaagaactg
     9361 cgaactctcg cagttctttt tttaaaatac gggataaagg aagtttccag cagacaagag
     9421 aagaaagcaa aagaaattgc tatttgattc acaaggttct ataacgaaat cgcaatcaat
     9481 caaacatata caaagaaggt tattcaaatg gaaatctgat ttgatgatgc ttctgctgtt
     9541 gaaataaagc agcatctagt acctgaaaac aaactaatag taacattcga agatggagta
     9601 ggtccgtatt ttcagcatgc aatgatccat atgtaagttt aattcatcct tgaaatcatc
     9661 ggaccagata aagttatgac caatctatcc caagcaatct agaagagata ttagtaaaag
     9721 aacatttact ggaagtctta gatgagccta taaatatatg ttctgatggc gtcatcagtg
     9781 gtctgattga cggaagctta ggtgtgatcg atttctctgg accaaacaac gaaggaatca
     9841 aggggaatcc ggcacgttaa gaaaaaaagt ataaaaaact agacacttga tgagcgttca
     9901 ttcaattgat tgaagggact aggctggtgt cattttttat acatatattt tctctttcgt
     9961 cttgataggg cttatgggat aatataagga agaaaatcat cgttttattc aaaaaggagt
    10021 ttttgacaaa tgaaaaaacg tatattattt atcggtgaca gcattacaga cgcaggaaga
    10081 gaccgagcag aagcaactga tctaggtaaa ggttatccac ttttgattgc cggaaaacta
    10141 atctcccaat atccaacgat gaaatttgaa tgcatgaacc gtgggatagg aggagacaaa
    10201 attgcagatc tgcaagtacg ctggcaagaa gattgtttag ctttacaacc tgactatgta
    10261 tcgattttga tcggaatcaa tgatacctgg cataatacta gcgatcaggc tatattcgga
    10321 actgagaaat ctgcgcagca atttgaatcc gtttatcgaa aattactaaa tgaacttcaa
    10381 acagcggaaa tctgcaacat catcttgatg gagccgtttg tacttcctat aaccgacgac
    10441 cgtcatggct ggcgtaaaga cttagaccct aaaatccaaa ttgttcgtaa acttgctaag
    10501 gaatatggcg cattgctggt tcctttagat ggcatattca atgctttagg gattgcttat
    10561 ggtttttcga aatatacaag agaagatggg gtccacccaa cgattggcgg acatgagatc
    10621 attgcacaaa gctggataga tgcaattgcc gcagtcgatt tttttagtgg agataaaaat
    10681 gagcgaaaga aaagtgagaa atgatggaaa tacggtgggc agtaaaagaa gatttacctc
    10741 aaatcaatca gctgattact gatcatccag aatcattcgt aaaagcaaaa acggcagctg
    10801 acctaaatga taagaataaa ctattgctcg ttgctgtaac aaaagaaaaa cggattatcg
    10861 gatttgcgca agcggacgtg caacagtcag aagaacgggt ggatcagcga ccatcaggaa
    10921 aactaatcca acacacatta gtaacaaaag aaaatacgga agaaatccaa aaaaaatttg
    10981 cagaatactt gatgctggaa gctaaaaaaa gaagaataaa atcaattttt gcccagtttt
    11041 aatcaaaaac agtaatagaa ggagaaatac aatgagttgt ttaggaaatg tcatctggtt
    11101 tatattcggc ggactaatcg gtggtcttag ctggttagca gcggggatac tttggtgtat
    11161 cacgatcata ggtatcccaa tcggtatgca atgttttaag atcgcctccc tttcttttgc
    11221 gccttttggc aaagaagtaa tttatcacca gtctggcatg tcattgatcg ttaatatcat
    11281 ctggctgatc gtttcaggcc taccaattgc tctcgcacat gtcgtcagcg gcatccttct
    11341 atgtatcacg atcataggga taccttttgc caagcaatct ttcaaacttg ctcgattggc
    11401 gttgatgcca ttcggtgcag aaattgtata aaaagagaat aataaaggtt agaaaacgca
    11461 tagagtcagg tgggatacat gtatttcacc tgactctatg tgttttcctg tataagttct
    11521 cccatgtgac ttttacgtct tttatttata tgggattact gtttatggaa agttgaaata
    11581 attttcatta aagaactctt tctatatttt tgaaagcgta gtggagcttc gaatatctgg
    11641 gaaggacatt ttgcagggat tgaaaaaaga tattgtccta tcgaaaaatt aatgaattta
    11701 gacaaattgc cagcaacagg cttcaaactt agttgctatc ctgtcaagat caagaaggca
    11761 agtgcgggtt ggataagagc aggggcaatg atcgaagaga aaaagaaaga ataagccaaa
    11821 acatctgtat ggaagaaatt ttccgatgca gatgtttttc attttttctt tcggatacct
    11881 tgacatatga ataaccattc atttataatt agttatgaag agatgttcat atgttgaggc
    11941 gaggaggtca ttaaaatgaa aagttacaga ttagaaggtt tagattgtgc gaactgtgca
    12001 atgaaaatcg aaaaaggagt tcagaagatc aatggagtca aggaagcaac ggtaaacttc
    12061 acttctggaa aactaacgat tgacgcagag gaagatcatt tggctacgat tgaacaagaa
    12121 acaaaaaaag tagtgaagga attagaacca gatgtcaaag taacagaaat agataaggaa
    12181 aaagtatctg aacatggaaa tgaaaaagag agaaatacgc tgttccggat tttattttcc
    12241 cttgcaggta tcgctttatt gctattattt gattttaatg aaccaattcg cttgataggg
    12301 tatttattga tttatctgct gataggatat gatgtagtga agaaggcagt gatgaatatc
    12361 gtcaaaggaa aaatctttga tgagaatttt cttatgtctg ttgctacgat tggcgctatg
    12421 ttgatcggtg aatatccaga agccgtagca gtgatgctat tttatcaaat cggcgagtat
    12481 ttccaaggat tggcagtcag tcattcaaga aaatcgattc gcgaattgat ggcgatccgt
    12541 ccagaaacag ctcacgtgca gacgacggaa ggattgatga ccgtgaaccc tgaagacgtg
    12601 cagatcggtc agtttgtact tgtgaagcct ggtgaacgtg taccgttaga tggaacgatc
    12661 atcgaaggag aatcattagt cgatacgtca gcacttacag gagaatcagt ccctaaatct
    12721 gtatatgttg gtgaaacagt attaagcgga tttatcaata aaaataaacc atttttagtt
    12781 caagtagaaa aatcttatga aaattctact atcagcaaat tattagaatt agtagaaaat
    12841 gccagcagta aaaaagcacc agctgaaaat ttcattacta aattcgcgcg ctattacaca
    12901 ccagtcgttg taggattggc cgttttatta gctgttttac ctccacttgt tgtttctgga
    12961 gcggcgtttt ctgaatggat ctatcgagca ttgacatttt tagtgatctc atgtccatgt
    13021 gcattagtga tttccgtgcc acttagtttc tttggcggta tcggaggcgc aagtaaaatc
    13081 ggtgtattag taaaaggttc aaattacttg gagttgcttg cgcaaaccga gacagtggta
    13141 ttcgataaaa ccggcacatt aacgaaaggt gacttttcga tccagacgat cgatacaaaa
    13201 atcgatccaa agaaattcat gcaatatatt gcgagtgcag aacagttttc tacacatccg
    13261 attgctcaat ctgttttaga aggctacgca ggtccgcttt taccaacaac taatatccag
    13321 gaatttgcag gtgaagggat cttagctgaa gttgatggca aacaagtatt agcaggtaat
    13381 cacaaattga tggaaagatt tgaaatctct tttcctttaa gtcaagaaat cggtacattg
    13441 ctttatctag cgatcgatca aagttacagt ggttatcttg ttattgcaga taccttgaaa
    13501 gaagatgccg tagacgcatt ggttcaatta aaacaggctg gtgtgaagaa cacagtcatg
    13561 ttgacgggcg actcgaaaaa aattgctgat catatcggga agcaagtagg cgtggacaag
    13621 atatatagcg aattgcttcc tgaagataaa gtgcagcggt tagaggaaat cttacaaaga
    13681 aacaataaaa agaccgcatt tgtcggagat ggaatcaacg atgcaccggt gctagcaaga
    13741 gcagatgtag gaatcgcaat gggtggattg ggaagcgatg ctgcaattga agcagcagat
    13801 gtagtcatca tgaatgatca accatcgaag attgcagaag caatccattt agcaaagaaa
    13861 acattgaaaa tcgtcaaaca aaacattgtc tttgctattg ggataaaaat tttggtactt
    13921 gccttaggtg cttttggttt tgcatcaatg ggagatgcag tatttgcaga tgttggggtg
    13981 acagtattag ctgttttgaa tgccatgcga agcttgcatg taaaaaaaga ggacgaatga
    14041 acgtcctctt ttttttatat ccttttctta atgaaaaatg taaagaaaat agataggatt
    14101 ataaaataac taatgttttt aattttgcag aaaattcaaa atagcttgaa attcaataag
    14161 gtcaatgaaa taaaagaaaa taacttattt ttttgatgga aaaaatatga aaaaacgatg
    14221 tgtattaaat aaaagaatac acacataaaa taacactgtc ttgtaagttt gttaaaaatt
    14281 gaatatgatt acatctggat aacaaatttt tatcataaag gagagaacga atgaacaaca
    14341 aacaattacg ctggtttact gttgcgttaa ttgcatttaa catggtttgg ggcatgggga
    14401 atgtagttaa taactatgct cagcaaggag tatcagttgt cacctcttgg ctattgattt
    14461 tggccattta ttttattcca tacgcattga tcgttggaca attaggatca gccttcaagg
    14521 atagtaaagg aggcgtcagt tcatgggtcg aaaatacgac atctaataaa cgactggcat
    14581 actatgccgc ttggacttac tgggtcgtcc atattcctta tttggcacaa aagcctcaag
    14641 caattctgat tgctggtggt tgggcagtcc aaggaaatgg aaatatcgta aatacgatgt
    14701 cagtacaaat ggtagcagtt atttcactta ttatatttct tgccttttta tatttatcaa
    14761 caaaaggact ttctactttg aaagtgattg gcggactggc cggaacagcg atgttcgtca
    14821 tgtcgatctt atttattctt ttagctgtaa cggcaccgtt catcaatcca acgatggaat
    14881 ttgccacacc acatatgaat caagtgaaaa catatattcc aacatttgat ttcagttatt
    14941 tcacaaccgt ttcgatgcta gtatttgcag ttggaggggc ggaaaaaata tccccttatg
    15001 tcaactctac aagaaatcct gcaaaggaat ttcctaaagg gatgatcttc ttagcagcaa
    15061 tggtagggat ttgtgctgta ttaggctcag tcgcaatggg tatgctgttt gctagtgata
    15121 atattcctaa agacttgatg gcaaatgggg catacagtgc ttttcaaatt ttaggtaatt
    15181 actatggtat cggtaactca ttgatgatca tttacgcatt gacaaacatg attggacaaa
    15241 tctccgcatt agccttttca attgatgcac cactaaaaat cttgttagca gatgcggatc
    15301 cagaatttgt accaaactgg ctacgtaaac gtaatcgtaa aggtacattg gtgaatggtt
    15361 atcttctgac aggtatacta gtaggaatca ttattttgtt gccgattttt ggtatcaaag
    15421 atatgaatga attagtaaaa tggttgacga acttgaactc agttgtcatg ccaatgcgtt
    15481 acctatgggt gttcttggca tatatgatgt tgaataaagc ttataagaaa ttcaattctg
    15541 aatataaatt tgttaaaaat ccaaaaatcg gttttatctt tggtgcttgg tgctttgcct
    15601 tcactgcgtt tgcatgtatc ctaggaatgg tgccaaaagt agattatact gctgatccta
    15661 aagcatggtg gttccaatta tttacgaata tcatcacacc aatcgtcttg atcttattag
    15721 gtatgatctt gccagcaatt gctcgtcgag acaaaaacaa agaaatcact gaaccagaaa
    15781 cagtggttca gccttaaaat agaaatactt gttacaagag gttgtgacag aagtggtcag
    15841 cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg aagtttcggc
    15901 ttgtttttcg aagaagctac ttctggaaca ccgtgaatca gaggttgtga aaaagctgtc
    15961 ctgcatcaag taataagaac agccaaacaa aaatagcccc tcatattttt cgtttggctg
    16021 ggcttattac cgcagatgca agctttggaa caccgtttaa ggaagaggtt gtaaaatcag
    16081 cgatttgacc tgagtattag agaaacaaac ggaaaaatag ctcctcatat tttgccgctt
    16141 tttgagctaa tgctgaaggt cagctgattg aacaccatt
//
LOCUS       DAZKOR010000062        16163 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000062 DAZKOR010000000
VERSION     DAZKOR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.062"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..420
                     /gene="scm"
                     /locus_tag="ACG8ZM_001907"
     CDS             <1..420
                     /gene="scm"
                     /locus_tag="ACG8ZM_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0492855.1"
                     /translation="STDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(575..1561)
                     /locus_tag="ACG8ZM_001908"
     CDS             complement(575..1561)
                     /locus_tag="ACG8ZM_001908"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0492856.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1584..2150)
                     /locus_tag="ACG8ZM_001909"
     CDS             complement(1584..2150)
                     /locus_tag="ACG8ZM_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0492857.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2164..2580)
                     /locus_tag="ACG8ZM_001910"
     CDS             complement(2164..2580)
                     /locus_tag="ACG8ZM_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0492858.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3037..4971)
                     /locus_tag="ACG8ZM_001911"
     CDS             complement(3037..4971)
                     /locus_tag="ACG8ZM_001911"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0492859.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5273..6208
                     /gene="pplA"
                     /locus_tag="ACG8ZM_001912"
     CDS             5273..6208
                     /gene="pplA"
                     /locus_tag="ACG8ZM_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0492860.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6298..7401
                     /locus_tag="ACG8ZM_001913"
     CDS             6298..7401
                     /locus_tag="ACG8ZM_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0492861.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7414..8367
                     /gene="menA"
                     /locus_tag="ACG8ZM_001914"
     CDS             7414..8367
                     /gene="menA"
                     /locus_tag="ACG8ZM_001914"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0492862.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFTMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9181..10527)
                     /gene="gor"
                     /locus_tag="ACG8ZM_001915"
     CDS             complement(9181..10527)
                     /gene="gor"
                     /locus_tag="ACG8ZM_001915"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0492863.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10661..11914)
                     /locus_tag="ACG8ZM_001916"
     CDS             complement(10661..11914)
                     /locus_tag="ACG8ZM_001916"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0492864.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11920..12843)
                     /locus_tag="ACG8ZM_001917"
     CDS             complement(11920..12843)
                     /locus_tag="ACG8ZM_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0492865.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13064..15556)
                     /locus_tag="ACG8ZM_001918"
     CDS             complement(13064..15556)
                     /locus_tag="ACG8ZM_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0492866.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15563..16030)
                     /locus_tag="ACG8ZM_001919"
     CDS             complement(15563..16030)
                     /locus_tag="ACG8ZM_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0492867.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4747 a         3377 c         2873 g         5166 t
ORIGIN      
        1 agcacggaca cgtcagcttc aagtgacagt acagatacta cctctaccac aagtgatagc
       61 tcagattcat ctaccactac aagtgatagc acggacacgt cagcttcaag tgacagtaca
      121 gatacatcct ctaccacaag tgatagctca gattcatcaa cttcaagcga tagtacagac
      181 acgtcgtcta ccacaagcga tacacgtcat gcaagtgtct ccttctcaaa aacaagttcg
      241 gataacagga cgaactttaa cttaactaat aaaacaagca atgacaatgg aaacaaatca
      301 aaaagagcac ttcctaaaac tggaagtcaa tcgaataatt ggataacact cgcaggcgtt
      361 atattacttg taatcggttt aagaatcact tttatgagtt ataagaaatc gaaaaggtaa
      421 aaaaatattc aagataaaaa agagcaacgt cgctaacgga cgttgctctt tatttttgct
      481 ttttatcata aatacaagaa ctacaattca atttgtcaaa aaacacgtag atttccttct
      541 gagaaattta tttacgtatc gaacctttcc ctattcagtc tttgcgatcc aaaatcagtt
      601 gggtaaggga aaagagattc tcttttgttc gaagagaggt ggcaggtaat ttctttattt
      661 ccttcaaagc cttttctgtg taagaggccg ctaacttcct agcttcttcc acgcctcctg
      721 tatgttcaac gattttgcgt acggcgtcga catcttcttc tgtcattgcc gctttcttct
      781 ctaaataagg tagtagcttt ggtttgtttt tttgcaaaga gtagatcaac ggtaaagaat
      841 agaccccttg acgcatgtct tctagtacag gtttgccgat ttcctggctc tcttttgtat
      901 aatctaaaat atcatcgata atctgaaagg ccatcccgat agaaagtccg atatctcctg
      961 ctttttttgc gaagcgctcg cttgttccac tttcaaaagc gccaacagaa caacttaatt
     1021 ggaacagttc agctgtctta cctgaaatat tttccaaata ttcttttaca gaaagttcat
     1081 aattatagcg atcatccatc tgccctaatt cgccattcaa tactttttcc atactgcgag
     1141 aattcaattg gagacttttc aaggaagtgg agtaatcaga caatagttta aaacagcaga
     1201 caaataaata gtctcctgca tagacagaaa cgacattacc aaactgacta cggatattag
     1261 gctgaccacg tcgcagatcc gcttcatcaa cgatatcatc atggatcagt gtggccgtat
     1321 ggagcatttc gatagaagct gccaaagcga tggctttgtc tcgatcttgc tctggtccaa
     1381 attgtgagaa caatagctga taggcaggac gcaacagctt tccgccggca tggatcgttt
     1441 ctttgattgc ttgttcgaca ggtttgtttt tcaggcggac agtttcttcc atccgtttta
     1501 gtgtttctgt caattcttta cttaaagcag gatagttttc ccacattgga tgtagcttca
     1561 taaaaaaact ccttgtgtga cgattatttt ttggtaaaca ttgggctcca tttttgtttg
     1621 gttccctgct cataatcttt ttggaatcgt tgaagtgtct gcacatgtcg taaaagataa
     1681 ttcgctgcga tcccgattgc aatccctgat aaaatcccta aaactgaaag gactggcaag
     1741 tacaacatga ctgtccatga ttgtgcaatg aaagaagcca tcaaaagctg tccgacatta
     1801 tgcatgaaac cgccggccgc actaattccg atgatactga cacgttttgg tcctaattgc
     1861 ttgaccaata acattccaaa ataactaagc aaagcaccac tcatactata aaggaatgtg
     1921 gaaatcgttc cgcctagcaa agtagtcagg aatagtcgta gccaaagtag taagaagctg
     1981 tcttgttttt tcatggtaaa aatggcaacg atcgtgatta gattggctaa gcctagtttt
     2041 gcaccaggcg caaaagaaaa aggataaggg atcatatttt ccaataaacc aatgatcact
     2101 ccttgtgcaa caagtagcga aatataaatc attttttgta atttactcat ggtgtgtaaa
     2161 ggtctaataa atcagactgt cttcgtcacc tccatctgtt gaacggactt cgatcactaa
     2221 tttatgtggc aaacagacga tggtttcgcc atttttcgtt gcccagcctc gacgtacgca
     2281 gatctggtca tcgcaatctg cttcttttat ccgtactcga tcgccatcta tctcgatcaa
     2341 gttataatct ccgtgttcat ctttgtaagt atatgtgttg ctctcttctc cagccatcaa
     2401 agggaatgtt ttgatttctg tgccatccac acgcagtacc gcttcttttt ctgctgttgc
     2461 ggtgttttgc tgaagaccaa aaatcatgac tggtaaaaaa gaaagaacag tcaaaagcaa
     2521 aataataaaa acatcccaag gtttgagcag acttttttca gcaaattctt taaggctcat
     2581 ttttgttctc ctttttccag attaactcac ccaattttag cataaaaaca agaggttgtg
     2641 acacctgtgt cttgctttga gtaataagaa cgaagaatag aaaacagctt tccatgtttt
     2701 ctaattcttt gggcttatta ccgagaagca gcaggtggaa caccgtttat aacaagaggt
     2761 tgtgacagaa gtgggcaact tcaagaaata agaaggaatt tacgaaaatt acttttcaag
     2821 tttttgtgaa atttcggctt atttttcgaa gaagctactt ctggaacacc gtttataaca
     2881 agaggttgta acagaggttg tgacacctgt gtcttgctca tggaggctta tctcgggcat
     2941 cttattcctt taattttaca taaaaaggag gctgtgacgg tttgtctcag cccctttcta
     3001 ttgaaatctt gcattcaaat aacgttcttt atctatttat ttcttgccgt actgggaact
     3061 tggtctaccg taccaccaat tacctagtgt acgttgtagc catggaatta cccagcgatc
     3121 caacccaatc gcacgtccgg aaccgttcat caatgcgaaa gcaacaaaaa tgaaccaaat
     3181 gtttacccag taaaacatgc ctgataagca gaacgtgatc accaatacaa tcgttgtcgc
     3241 actgcatagc catgtgaata atccagcaat caatgctaat gccaaggcaa tttcaaagaa
     3301 taccatgaat ttttggaaga acattgctac atcattgttt ggcatgatca cttgcatgat
     3361 ctttgtgaac cagtctggtg acttctcaat cacagccatt ggttcttcac cgtatgcata
     3421 actcaaacca aaatgtgcag cttgagtcgc tgcttctccg gcacttgccc ctgctccgct
     3481 ggcagctgtg gttgcatcac cagcagcaga agcaccactg gcaacatctg ttacattgta
     3541 ttcccatgtc caagggaaca cactgtttcc agtgaaccat gatccttcac caaaccaagt
     3601 acttggtttg aacacttcac cattcccaat gatttttttg gaagcttcaa cgaaccaaac
     3661 taaaccatag aaaatacgaa gtggcacact ccataagaca ttgccgtaac gagaagaatg
     3721 tcctcgagtc acgttgcgat catctttgat atggaaaaac tcatgcatta aatattggaa
     3781 catataataa ccagaacgaa tatcaaagaa gtatttcaaa ttcacgatat gtttcattac
     3841 gatagctaaa aatccgctta aatggatctt atcaaatagg ttagctaccc cccatttggc
     3901 accaatagaa accataaatc cttgatagtt tcctttaaat ttatgctttt ctccgccttt
     3961 gatcgaagcc acgatattcg ctgcggctgt gtgtcctgtt tgttcagcag cttgaacgat
     4021 ctgcggtgtc ggtgttcctg ggaattcttc ataaaagact aaatcaccaa tgatatagat
     4081 gtttttatct tcgtagcctt ttgcctgcat atattcattg gcgatcaacc gctgtccgcg
     4141 tgctgattct aatccaaagt cagcagcgtc agaagttcct ttgacaccag ctgtccaaat
     4201 cagtgtgtga gtcggaatgt tgcgtccgtc tttcaatttg atatgatcag cagccacttc
     4261 tacgattggt gcattcaata ataattccac gttgtgctta cgcaaatagc gttcagcttt
     4321 tgcagcatca tttcgatcca acatattcaa gatcgtcggc attgcctcta cgaccatcag
     4381 tgtgatttcg tttggatcta atttataatc tttggctagg cgatttttcc agtcgatcag
     4441 ttccccaatc atttcaatac cggtaaatcc agaaccacag acaacgaaag tcagcatggc
     4501 tttacgcagt tcatcatctg gttccaatgc agcttttgct actgtcttct caatatgttc
     4561 ccgaatcctt acagaatctt caaatgacca aagggtgaat ccgtgtgctt tgacaccagg
     4621 tgtaccaaag tcattcggct ctccgcccat tccaaggatc agctgatcaa aaggatagga
     4681 acctaatttg gtcttgacga tttttcgttc tttatcgata ccgattacag tgtctgtgac
     4741 aagttgtaca ttcttttgac gagcaaacaa atgctgcaga tcgtattgga ttgcttctgg
     4801 ttctacacgt ccgccagcta cttcatgcaa ctcggtcatc atagtatggt acgaatgacg
     4861 atcgatcaga gtaattgcta catcttcttt tttcagtttc tttgccaagt attttgtagc
     4921 tgaaacaccg gcatatcctg ctcctacaac gacaatattt tgctttgcca ttgatcatcc
     4981 gctccttata gactatctat ataatgaata tatgataatt cttttagttt gatacttatt
     5041 acacaatctc catatattat atagatgttc tgttcttttg tctaactcct tttggatttt
     5101 tttcaaattt taaaataaag cgttttttca cgaaatttgc tgaaagaaaa cgtttgaaat
     5161 attacatggg ttatttatca aaaatagttg acttataggc taaaaagatt agaatgtata
     5221 atgaatcaca aagaaaaatt gtggactaga aatggaatgg tggggtatta aaatgaagaa
     5281 aaaacgtttt gtaactggat ttgccgtatt agcattttca gctctagtgt taggcgcttg
     5341 cggcgcagat gacaacggaa gctcaaactc ttcttctgaa tcatctacag cacaatcttc
     5401 gactgcaaaa agcacaacag aatcatctgc aaaagttgta gctggcggag atttgcaaga
     5461 cggcacatat aaattagaag aaaaaaatta ttcaaatggt taccgtgcag tctttgaaat
     5521 ggttgtaaaa gacggcaaaa tcactgaatc aaaatatgat aacgtcaatg aaaacggcga
     5581 atcaaaaaca aaagatgcag aatacaacaa aaacatggaa gcaaaatctg gcacaaaccc
     5641 agaaaaatac attcctgagt tgaatgacca attcttaaaa gcacaaagcg caagcggtgt
     5701 ggaagtcgta actggagcta ctcactcatc tgaaagcttc caaaactacg cacaacaatt
     5761 gatccaagct gctcaagctg gtaacacaga tacaatcgaa atcgacaatg gtgctgattt
     5821 gaaagacggc acttacaagc ttgaagaaaa gaactactct aacggctacc gtgtgcaatt
     5881 cgaaatgaca gttgctggcg gcaaagtaac tgaatcaaac tttgattata tcgacaaaga
     5941 cggtaaatca aaacaagatg atacagaata taatgaaaat atgaaagcaa aaagcggtac
     6001 agaaccaaaa acttacatcc ctactctgaa cgacgaattc gtcaaagcaa tgggcgaaga
     6061 agatggctca cctgctaatg tagaagttgt tacaggtgct acacacagct cacattcatt
     6121 catcatgtac gcacaacaat tagtgaatgc agctgaaaaa ggcgacactc aaacaatcga
     6181 agtagataat atcgtaacag aaaaataatc acataatcat actaataaaa gttggagggt
     6241 gggacgatag ccagattagg cagtcgtctc acctttcaaa atgaagagag gaaaaagatg
     6301 cggaaaaaac tactattttc cttaatcaca ctgggcatct cttttatggt gctaactggc
     6361 tgcagcaact cagcaaataa aacaaccact gaaagttcag cagctaccaa gatattgaaa
     6421 gatccatatt ctgatgaaca gttcttgtta gggacttacg ttcgtatccg tgtgtatgat
     6481 gaaggcaaag aatcagcatt gaaacctgcc ttcgatcgag taaaagaact tggtgataaa
     6541 atcacgatca atcaaaaagg ttctgagatc gatgaagtga atgagcaagc tgggatcaaa
     6601 ccagtaaaag tttctgatga cgtctacacg ttagtcaagc gggcctacga atatagccaa
     6661 gattctcaag gcggattcga tatggcgatt ggtgcaatca cacaattatg gcgaatcggt
     6721 tttgatgacg ccagaaaacc ttcccaagaa gaaatcgatc aggcattgaa attagtggat
     6781 tatcataaga tcgaactaaa tgacaaagaa aaaactgttt atctaaaaga aaaaggaatg
     6841 atcatcgatt taggtgcaat cgccaaaggt tacattacgg acgaagtagt gaaagtcttg
     6901 aaaaaacaag gagtgaccac tgccatcgtt gacttgggcg gtaatgtcta tgtcttaggc
     6961 cacagcccaa gaggcgaaaa ccaagactgg acagtcggca tccaagatcc gaacatggct
     7021 cgcggatctg ttttaggttc tattaaagaa cggaacaaaa cattagttac ctctggaatt
     7081 tacgagcgtt atttagaagt agatggcaaa aaataccatc atttgtttga tccaaaaacg
     7141 ggttatccgt tcgacaatga tatcgcaagt gtcacgatca taacagataa atcaattgat
     7201 ggtgacgggt tgtctacagc agtcttttca atgggtgtga aaaaaggact ggaatacgtc
     7261 gaaagcgaat tgaataacgg gacggaagcg atcttcgtga caaaagacga taaagtatat
     7321 gtgaccgatc caatcaaaga caccttcaaa ttatcggagg actcccatta cacgatgggt
     7381 gaccgttctg aactaaaata aggagtacga accatgacac taaaaatctt tttagaagta
     7441 gttgaaatac gaacaaaggt ggctagtgta tttccttttg cggtaggggt attattctct
     7501 atcgcttttt ttcatgagat acattggcta aacacttttt tattttttat tggaatgcta
     7561 gtttttgatt tagcgactac cgctatcaat aattacatgg attatgaaaa ggctcattct
     7621 gaagtatata agtatgaaga aaatgtcatt ggccgcagaa atctatctcc tgtattgatt
     7681 cgaaacatga ttttagctat gattgcattt gtttttgtta tcggcgttat tctaacgatc
     7741 aatacgggtt ggcttttact tttgatggga tttatctgtt gttttattgg agtattctat
     7801 acattcggac cgattccttt atctcgaatg cctttagggg aaattttcag tggggtgact
     7861 atgggccttg gaatttttac catggtcgtt tatgtcaata cattcggttc agatgtctat
     7921 gatctgacag tcaacttttc atctggacaa tttgctttgg taggaaatct ttgggctata
     7981 gcagcaatcg tattagcttc cttacccttg gtgtttacga ttgccaatat catgttggct
     8041 aataatttgc gggatcttga ccgtgatatt gaaaatcatc gctatacact agttttttat
     8101 atcggacgtc ctgcgggaac agtcttattc cagctattga tgtatgcttg ctatttcgtg
     8161 attttaatgg ggttgttgag cggtatctat gaatggccga tcttggtcac cttcttgacc
     8221 ttgccaattg tgaagaagca tttggatcaa ttcaagcaaa cgttacccca gcctatcagt
     8281 ttcagctatt ccatcaaaaa tatggtctta ttcaatgcca gttatgtatt ggggctatta
     8341 ctaacaatca ttatgcaagc catttaacag catgaagagg ttgtgaaaaa actgccctgc
     8401 atcaagtaat aagaacagcc aaataaaaat agctccttat atttttcgtt tggttgggct
     8461 tattaccgca gatgcaagct tttgaacacc gtttatcagg aggttgtgaa aaagtcgctt
     8521 agtttcgagt attaagaaaa attttgtgaa aatggctttc cacatttttg cataaatttg
     8581 gcttaatacc gagaaactgg cttttgaaca ccgtttatca gaggttgtaa aatcagtgtt
     8641 ttgtcctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
     8701 agctaatacc gaaggacagc tgattgaaca ctatttgttc ggaaaaagtg actaactatg
     8761 acacaatttt tttcacagtc ccttttttca cttgatttga ttcgtaccag cgaacttgac
     8821 gagaactttc ttggtttggt aactatatgg tattctttta acatcctcgt ttgcttcttc
     8881 taatttattt atacaaagtc acactttttt aaacatctgt tcttcgatac atttctattt
     8941 cttttaaaca tcgataccaa tattttgaac ttaagagtgc ataccaaatg gattcattta
     9001 ctcttcgtca gttacgaaga taatcaaaat ctccaacttc cctacttatt tcgttttgag
     9061 aattatcgaa gcaatcttac ataaataact aaaaaacaaa aaaccagaaa atcagtatac
     9121 tgattttctg gttttacatg gtttgttcaa taaaaagctg ttggaactgt taatattaaa
     9181 ttatcgcatc gttacgaatt cttccgctcc tgttggatgg atggctactg tattgtcaaa
     9241 gtcttcttta gttgcaccca tcttgatagc cacggcaaaa ccttgcagca tctcatcgac
     9301 accaacacca atagcatgta agccgacgat tctttcttct tttccgacac agatcaattt
     9361 catttcgcat ttttggcgat aatcatttaa tgcaaagtac attggggtaa agcgtgaacg
     9421 ataaactttc aattcatttt cgccatattt ttccaatgct tcttcttctg tcaaaccaat
     9481 tgttgcaatt ggtggatgcg taaagacgac tgtcggaaca agtttatagt caagataaga
     9541 atctttcttt ccattaaata aacgttctga taaacgtcta ccagctgcga ttgcaacagg
     9601 agtcagatct aatttaccga tcacatcacc aacagcaaaa atatgttcat ccgttgtatt
     9661 ttggtattta tctactttga taaacccttt tttgtccaat tccacatttg tattttccag
     9721 acctaatttt tcggtattcg gtacacgtcc accggcaaaa agtacacaat caccagttaa
     9781 ggtttcacca ttttcaaata gcaccgtata ttcgtcattg tttttagtaa tttctttagc
     9841 aatatgatgt gcgtaagttt ttattccagc ttcttggtac atttggatca aattgtccga
     9901 aagcatttta tcaaacgttc taagtggtcg ttcttttcga tatgcccaca tgacttcact
     9961 acctaaacca cgaaatacac ctgaaagttc agccgcaatg tatccagcac ctaaaacgat
    10021 tgctctctta ggtaactctg ttaattcaaa aaatccattt gaatcaattg cgtattctcc
    10081 accaggaatg gtcattttac ttgcacggcc tccagtagcg atcaagattt taggagcacg
    10141 ataggtttca ccgtttactt cgatcgtttg actatcgata aaagtagcgt aaccttttat
    10201 agcctcgaca tgattgctat ctaatcctct ttgataagca ccgtgtagaa aatcaatata
    10261 tttttctcgt ctttctacta gaccggcgaa atcaacattt tttaaatctg cttggatacc
    10321 gtaacttgag gcatctcgtt caattgtttc caaaatcgta cttgcttgcc acataacttt
    10381 tttaggtaca caaccgacgt tgacacatgt tccgcccaat tcattcgctt caatcagcaa
    10441 aacttttgcg ccatgcatcc ctgctctgtt tgctgaagca ataccgccag agccgccacc
    10501 aattacgatg taatcatata tttgcatttt gttcctcctt attgaagaga tcaatcaaaa
    10561 cctttattta acgatccttt ttctacagaa aacaagtgta acacttttct tgaattttgt
    10621 atgcgaagat gcttaaaaca gggatatatc agctttttcc ttatttacgt tttcggatca
    10681 tatctccagg tttcaccggc tgtgcgactg gcatagttaa aatcatcatt ggatttggtg
    10741 ctcggtcaat ggattcgcct tcttcattat gcataacttc aacggtttga ctgaaatgac
    10801 tgaatcctgg accataaaat tcgatttcat ctcctacaga aaacaagttt ctctgacgta
    10861 tcgtagcaat ctgtgttgcc tcatcataag ataatacttc accgacaaat ttatatactg
    10921 ggattttacg acgttcaccg aataattgtt cgttttcagt tggtgtattg tagtaaaaac
    10981 ctgtagctaa ttctcgttgt gctactttcc ataactcgtc gatccattct tgcttgcaga
    11041 catagttttc aggatcttcc atataactgt ctacagccgc tttatagaca tttgacaccg
    11101 tagatacata atggatcgat ttcattcttc cttcgatctt gaagctgtct acgccgtttt
    11161 caatcaattc tggaatatgt tcgatcattg acatatccac tgcactcatc gagaattctt
    11221 cttccacttc gcctgtatca gttaaactgt ttcgttcttg accaaaaggc atatcgaaca
    11281 atccatactt ccagcgacaa gattgagaac atccgccacg gtttgcatcc cgcatcgaca
    11341 tatggttgga caatgtgcat cgaccagaat aagagataca catggcacca tggataaatg
    11401 cttcgatttc ggtatccgta tttttacgaa tctctgccac ttcctccatt gaaacttcac
    11461 gagctaaaac gacacgttcc aacccttctt ctttccagaa ttccaatgtc tcaaaattag
    11521 ttgctgaagc ttgagtggat aaatggatcg gcagacctgg ggcttctgtc gcacaaattt
    11581 cgatcaaagc tggatcagaa acaatcactg cagaaatacc gacatcacgc aaactgcgga
    11641 aaaattcccc tgctccatct tcatttcctt catgggtgac catattagca gcaacgtata
    11701 ccttcgcatc atgttctttt gcaaaagcaa ctccttcagc catttcttca taagtaaaat
    11761 tacctgcacg gcttcttaaa ccatatgcat tcccgccaat atatactgca tctgctccat
    11821 aatggatcgc ggtttttaac ttttccagtg ttcctgctgg cgctaggatt tctggtcgtt
    11881 tcaattttct ttttactgtc atttctttca tcatactcct tatttgatag cagaaggatc
    11941 aatataaaag aatcctgtat ctaattcacg gttttcagga tgcaatttac tgatttcgct
    12001 agtcattttt tccgcttttt cttgatccca gctaccttct aaaatttgtt tttttgcttc
    12061 ggcgaatagt cgagtgatgt ttacgaattt ttcgcctgga gtaaataatc cgtccaattt
    12121 ccatgtacag taatgatgct gataaagttc attcaattca ttcgagagat tcagatcatt
    12181 gctggcaaaa atatgtgtgc catgactatc ttcataaata gaataatgtg tctcttcttt
    12241 tttaggttcc gagataaaaa gacctctttc acgatctttt tgttcgtctt gtttcgtata
    12301 gttatagtaa ttctgtagca gtggacgttt ggattgatgg atgcatgtag caccatacac
    12361 aagggtttct acaggaatat cgagtttttc ttccatcgcc ttcatctctt caaaaggaac
    12421 ttcacgagca agtactgctc caactgcgcc ttttttcgcc cagaaattga tttgacgtgc
    12481 gctcgttact aatgtttcag catcataaat atacggtata cgcaattcag gatttttttt
    12541 catcgtataa ataatacctg tatcaccaac tgtgatcaca tctacgttta tttcttttaa
    12601 aaaagccaaa tattcaggta catttttcat tttttcagga tgaatgatcc catttacagc
    12661 aatattcgct tttttaccat acttgtgtgc taattctacc aattgtcgct gttcttctcg
    12721 tgtaaatgaa gcaggcagac gcaatccaaa ttgctcttca ccgaaatata gtgtatctgc
    12781 acctgcagca agcaactgtt ctgcttgttc aatagtttct actgtggtta tgagttcgat
    12841 cataattttc tcctcctaca acttcataat agtagcatag ctctaaaaac tttccaatac
    12901 ttcttctatt taaagtcgga tttttgaaaa ttatttcaca aaaaatcaaa ataaagttcg
    12961 atatattctg tccaaatata aaaagagatt gtgacaaaaa tcttgtcaca acctcttaat
    13021 caagtcttac tattataaaa attcagctac acaggccaag taactatagg gaacctactg
    13081 tttccttttt aggtccgcga acattcaatg tgatcttacc ttttgaagca cctactgtta
    13141 cgtggtcacc taaacgaatt tctccgccaa gcaaagcctc gctcaatcga tcttcaattt
    13201 ctttttgaag tgcgcgacgg attggacgtg caccatattc aggatcaaat ccagctttcc
    13261 cgatcacatc gattgcagat ggtgtgattt tcacttgaat atcttgttct ttcaatcgtt
    13321 tgataatcgc tttgctcatg atttggacga ttgtatggat ttcatcttta ctcaatgaat
    13381 ggaagacgac agtctcatcg attcgattca agaattcagg tcggaaagct ttctttaact
    13441 cttctaagat acgtttttgc atggctttgt gatctttagt aacatcttgg acattgaatc
    13501 cgacattttt ttcttctcga atttgtgtcg ctccgatatt tgaagtcata atcatgattg
    13561 tatttctaaa gtccactttt ctacctttag aatctgttaa atgaccatca tccaatactt
    13621 gcagaagtaa attgaaaacg tctggatgtg ctttctccac ttcatctaag agaatcactg
    13681 aatatggttt ttgacgaatt ttttctgtca actgtccacc ttcatcatac cctacgtatc
    13741 ctggaggaga cccgattaat cggcttgtac tgtatttttc cataaattct gacatatcaa
    13801 cacggatcag ggcatcttca cttccaaaca tgacttctga aagtgctttg gccaattctg
    13861 tttttccgac accagttggt ccaaggaaca tgaatgaacc aattggacga tcaggatctt
    13921 tcaatccgct tcgtgcacgg cggatcgcac gtgaaactgc tttgacagcc tcatcttgac
    13981 caacgacacg ttcatgcaga agtccttcca gttctaacaa gcgttcgctt tcttttttct
    14041 ccaactgctg tagagggaca ccagtccact ccgatacaac tgtagcaaca tcttcttctg
    14101 ttacacgatc agaataacct tgctcctgtt ttgcttcaat tgcagaaact ttttgcagct
    14161 ttttacgtag tgctttttct tgtctgcgta attgagcagc attttcaaag tcttgttttt
    14221 ggatcgctgc ttccttttct tgtactagtt catcgatttc tagtttgatc acagttgatt
    14281 ttgttaagtg atcggtttga tccaaacgga ctttcgctgc tgattcatcg atcaaatcaa
    14341 ttgctttatc gggtagttga cgagaagtga tataacgaac agaaagattg actgctgctc
    14401 gtacagcttc atctgtgatc tccactccat ggtgctcttc atatcttgta cgtaatcctt
    14461 gaagaatcac ttctgcttct tcaggagttg gctcatctac ttgaatgcga gcaaaacgtc
    14521 gctctaatgc agaatctttt tcgatatatt tttgatattc atctagggtc gttgctccga
    14581 tcgtttgtaa ttctcctcga gctaacgccg gtttcaagat attcgaagca tcaattgctc
    14641 cttctgctcc acctgcacca attaaggtat gcaattcatc aatgaataag attacttgac
    14701 catcattata gatttcgtcg atgacctttt tcattcgatc ttcaaattca ccgcgatatt
    14761 tcgtaccagc aaccaaagat cccatatcaa gcatcatcag gcgcttttct tgcatgtctt
    14821 ctggcacttc acctaaaatg attttttgag ctaacccttc agcaatggct gttttgccta
    14881 caccaggttc acccaccagt acagggttat tctttgtccg tcggctaaga atctggataa
    14941 gccgtttcac ttctctcgaa cgaccaacaa ctgggtccag cttgttttct tttgctaatt
    15001 tcgtcaaatc acgtgctaat gaatctagcg taggtgttcc ttgcggtgtt tgccgtttat
    15061 cttgtacatt tacatttcta cgacgattca ttccttttcc agcagaatca ccagctagcc
    15121 ccattttttg tttcagaact tgacgggctt ttgaaaggct gatacctaag ttgatcatga
    15181 ttcgagtagc catgatactt tcatctttta ataagctcat caaaatatgt tctgttccaa
    15241 ctttttgaga accagttttc ttcacttcgc tgctagctaa agcaaaaact tgtttcattc
    15301 ttggtgaata tggaagaaag actcctttag ggtaagaacg gataccacca taaccaatca
    15361 gatgttcaat ttcttcataa atttccgttt cattcaaatc catttcacgt aatacttttc
    15421 cagcaattcc attttgctcg accactaagg ctagtaaaac atgttctgaa ctaactgatt
    15481 gatgtttcaa ccgttttgct tcttcttgtg caatttctaa tactgcttgg gctctttcag
    15541 taaaaagttc ttccatctaa tctcacttcc catcttcata acttaaacgt tccagaaatg
    15601 ctcggagaat ccgagcacgt gtatgatctt catattcatt gaaattcaat gtgttttttg
    15661 caattgcact caacatcaag tttccttctt tttttgtaat tatttggtct tcataaagtt
    15721 tttgaataat agcaaaagca tttttctcac tgattgtatc atcaaataac tgatttatct
    15781 gctcaagtaa ttgtttttta tctgaaatcc ttacttttgc aattcgtata taaccaccac
    15841 caccacgttt gctttctact agataaccac gttggatggt aaaacgggta ttaatcacat
    15901 agttgatttg tgaaggaaca cagttaaaga ggtctgccat ttcaatcctt cgaatctcga
    15961 tcatttcact attttccaga attttcttta tatatgattc aattaaatcc gaggtattct
    16021 gatgtgccat cttattcatt ccctttgact aatattgacc ttaattataa cgaatttgat
    16081 aatagaaaga aagaagaacg ctttttgtta aataaatcct ttagaatcaa caaaaaaaaa
    16141 tacggaatat actattccgt atc
//
LOCUS       DAZKOR010000063        15900 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000063 DAZKOR010000000
VERSION     DAZKOR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.063"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACG8ZM_001920"
     CDS             complement(52..522)
                     /locus_tag="ACG8ZM_001920"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0492868.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACG8ZM_001921"
     CDS             complement(681..1139)
                     /locus_tag="ACG8ZM_001921"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0492869.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACG8ZM_001922"
     CDS             complement(1310..2455)
                     /locus_tag="ACG8ZM_001922"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFQ0492870.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACG8ZM_001923"
     CDS             complement(2468..3385)
                     /locus_tag="ACG8ZM_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0492871.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACG8ZM_001924"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACG8ZM_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0492872.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="ACG8ZM_001925"
     CDS             4477..4647
                     /locus_tag="ACG8ZM_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492873.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="ACG8ZM_001926"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="ACG8ZM_001926"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0492874.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="ACG8ZM_001927"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="ACG8ZM_001927"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0492875.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /gene="dapA"
                     /locus_tag="ACG8ZM_001928"
     CDS             6911..7792
                     /gene="dapA"
                     /locus_tag="ACG8ZM_001928"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0492876.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /gene="dapB"
                     /locus_tag="ACG8ZM_001929"
     CDS             7789..8505
                     /gene="dapB"
                     /locus_tag="ACG8ZM_001929"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0492877.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="ACG8ZM_001930"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="ACG8ZM_001930"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0492878.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="ACG8ZM_001931"
     CDS             9222..10223
                     /locus_tag="ACG8ZM_001931"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0492879.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="ACG8ZM_001932"
     CDS             10220..11581
                     /locus_tag="ACG8ZM_001932"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0492880.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="ACG8ZM_001933"
     CDS             11541..12692
                     /locus_tag="ACG8ZM_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0492881.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="ACG8ZM_001934"
     CDS             12667..13836
                     /locus_tag="ACG8ZM_001934"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0492882.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHIKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="ACG8ZM_001935"
     CDS             14011..15390
                     /locus_tag="ACG8ZM_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0492883.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15671..15900
                     /locus_tag="ACG8ZM_001936"
                     /pseudo
     CDS             15671..15900
                     /locus_tag="ACG8ZM_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5203 a         2803 c         3383 g         4511 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat atcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact
    15781 aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg
    15841 aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DAZKOR010000064        15473 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000064 DAZKOR010000000
VERSION     DAZKOR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.064"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..1422)
                     /locus_tag="ACG8ZM_001937"
     CDS             complement(58..1422)
                     /locus_tag="ACG8ZM_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0492884.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1419..2978)
                     /locus_tag="ACG8ZM_001938"
     CDS             complement(1419..2978)
                     /locus_tag="ACG8ZM_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0492885.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3034..5514)
                     /locus_tag="ACG8ZM_001939"
     CDS             complement(3034..5514)
                     /locus_tag="ACG8ZM_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492886.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            complement(5498..5833)
                     /locus_tag="ACG8ZM_001940"
     CDS             complement(5498..5833)
                     /locus_tag="ACG8ZM_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492887.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5847..6881)
                     /locus_tag="ACG8ZM_001941"
     CDS             complement(5847..6881)
                     /locus_tag="ACG8ZM_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0492888.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6971..7651)
                     /locus_tag="ACG8ZM_001942"
     CDS             complement(6971..7651)
                     /locus_tag="ACG8ZM_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0492889.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7935..9038)
                     /locus_tag="ACG8ZM_001943"
     CDS             complement(7935..9038)
                     /locus_tag="ACG8ZM_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0492890.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9260..9730)
                     /locus_tag="ACG8ZM_001944"
     CDS             complement(9260..9730)
                     /locus_tag="ACG8ZM_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0492891.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            10063..11703
                     /locus_tag="ACG8ZM_001945"
     CDS             10063..11703
                     /locus_tag="ACG8ZM_001945"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0492892.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            11857..13179
                     /locus_tag="ACG8ZM_001946"
     CDS             11857..13179
                     /locus_tag="ACG8ZM_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0492893.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13241..13672)
                     /locus_tag="ACG8ZM_001947"
     CDS             complement(13241..13672)
                     /locus_tag="ACG8ZM_001947"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0492894.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(13736..14572)
                     /locus_tag="ACG8ZM_001948"
     CDS             complement(13736..14572)
                     /locus_tag="ACG8ZM_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0492895.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         4529 a         3122 c         2798 g         5024 t
ORIGIN      
        1 attctcgatt gattaatatt tattatatcc ccacacaata aaagaccgtg atatttctca
       61 cagtctttta tctactctat tgctatttgc tctcttagct agtactttaa aagctaactc
      121 attgaggtag tgaaaacgag aggtgtattc agacagaaat ccaaaatcct caactgctag
      181 ctgtggaatc gtagttttgt gtttcacgat atccgcaagc aggttcaaat ctgataataa
      241 gaattcttgg cttatcagct gtccgccaat tatttctaat gtatccggat ggtagatcag
      301 ctttgcattt actgttttgt tttcttcgtc gtattgtgga aatagatcaa attctcctga
      361 acacgaaccg gtattttgtt ccaagaatgg cgcttcatct tctgtcagcc ctacagttcc
      421 taaaaagtag tctataatga ctgctgctgt tatcttttgt ctgaggtcgt aagcggttgg
      481 ctgaccactc aaagttagcg ctactgctct tgccatatgt acagctcgag taactaaagg
      541 taaaaagaga tgccgtttat ttttgtcaaa actgactggg acgatatctc caatcgcata
      601 gatgtccgga tcacttgtct gcagatattc attgactatc acagtctcat cctcatacaa
      661 agccactttt tcttttaaca atgtcgtatt aggacgagtt tgggcagaaa aaatgaccat
      721 atctgccttt attgtttgag acagaagttg tacggatact actttctctt ctgatttttt
      781 ccaatcgatc aagaattcat ttggataaaa atttatttgc ttagtttgca gctgattttg
      841 aaaactgttg atcatatcct tatcgaaata tcttgataaa acattcggca tataatcaac
      901 tagatgaata atgtatcctt tatttctcaa agcatctgct aattccaatc caatgtaacc
      961 agcaccgaag atcactactt cttttgcttc ttttaaagcg gcaagtactt gcttagattg
     1021 cactagattt ttataggtgt agacattttc agcttcaatt tgtttataca aggtgacagg
     1081 agaggaacct gtcgctacga ttaatttatc ataggttatg gtctcgtttt ttccttcttt
     1141 ggatgcgagg atgagctgat gtttgtctgg atcaagttca gttactctcc attcggtgat
     1201 cagttttgcc ccactttctt gaagagtccg catgttatgg gcaacttcag ctaaactacg
     1261 tatctttcct tttaaataaa ggttgattcc atttgaaaca tagcctagtc catctttatg
     1321 atcttcatcg atcagtaaaa cttctgcttt cggttgaagt tttcttagat ttagggcagc
     1381 tgtgatgcct gcatgggaag caccaatgat cacgattttc atgttttatt catccttaaa
     1441 ttcaattttt ctaaataaca ttcttttaaa aattggttca aataggggaa atcataatta
     1501 tattcgttgc tagtgaatac aaaaacttta gcggacttct gtgacgggta ctctttcaat
     1561 tggctagtca aaatgatatc atactgcttt tctttctcgt attcgactaa atcatatttg
     1621 atcgtattca gcaattgttc ctggatgatt tctttcacga tatccgccat tagcctttca
     1681 taaggaagat cataagcaat ttgcagacgt aattctaact gcttgatcag cgtccgtaaa
     1741 atagatgcat agcgagcaaa taattcaaag cgggcatttt cctcgtctac ttcagtaaaa
     1801 tcaacaatag caaatgtttg tctgaccaat ctttcacata aaaccaggaa ttgtttatct
     1861 tctacttcag ttaaccgctg ttttaatcct tcttggccaa aaatattcat ccatcctcta
     1921 aaataggcaa aatgagcgtg attttgcatc actgtcaacg caatcttttg cgaaatctct
     1981 tctggaaaaa cataaaaaga gaaatattct tctatcgatt gtttaaacgt tcgattcaat
     2041 tgagctacta cgtcgtcttt gtatttttcg cttatcaaaa actcctggac atgcgagtta
     2101 tgtgtatcta atgtaaagtt actgatcatg aaaagataaa aaaccaacgt ttcattttga
     2161 ttccaaggaa aggcatattg aaagaagtaa cgggtcaaaa atgtatggag gtccaataaa
     2221 aattgtgtcg tttgaaacaa tccaacagga agattgctat aatctggttt ggattttttc
     2281 gtacagcgca ttcgcttctt cataatggaa agccatattt caaatttcaa tcgtccttct
     2341 tctgtaatag caaaactaaa ctctttttgc agcagatcta aaaaagtgtt tcctgataaa
     2401 tcactgtatt cttttttgtt ttcaagatag ggacgtccaa accaaaataa ttggaaataa
     2461 aaataacgaa tttgactttc tggaccggtc agccgcccgc tttttatttt cagatgaaac
     2521 tcatttaaag aatcatttaa ttccttcact ctgcgagaca aagtagccga actgatatta
     2581 agttccatga caagtttctg ccgatcaaat gagccatttt ccaacagata aagaagtatc
     2641 tgatattttg tagaatagcg caagtaacaa tgataataaa catcgactgc tgctatattt
     2701 ccatgtctga ttaataaaat attcccttct tggataacaa tttccaattg tttgatattt
     2761 tccaagttag cttgtaaata aaacagatct tctttcaagg tagcccgatc aactgataag
     2821 cgggtaagta cgttattaac ggaaacctct cctttttcct ctaataaaag ttggaaaagt
     2881 ttgatttgcc gttgttcatt tttttcaagc aaactgaaaa gttccaagtc cactccttca
     2941 agcatttctg attttaacag cagaagtttc cttctcatcc ttgaaaaaga ggatctgtga
     3001 cagttatcac agatccatac aaattctaat cacttagctg gttggcccag ctgtcagttt
     3061 ccatgtgacc atgccttgat acgttgcgcc aggttgagca ttcgtactgc tatttacctc
     3121 taagagtaaa ccatcggtat cgggaaagtc aacgagaacc tctcctttag ctgttccatc
     3181 actctcataa atcaacgtgt tttgaccttg ctgaatcaat tgctttgatc cagaatgatt
     3241 gaagtataat tttgttccgc ttaattcttg accttgatca ttcttaaata aagaagtcaa
     3301 tgtacctgtc agttgccaag gagacgtgct tggaagccga gtatcccgca catcaaaaga
     3361 aaaatcgcct tttgattcat aataacttgt cttcaacggt ttggggagat cgttaccaaa
     3421 tgtaaaatca tctggtacat taactaatgt taattcttgt ttggggacaa gaattgtttt
     3481 agcagatgtt tggacaactg ggttaggaag cttagcacca tctgctaatg taatattgct
     3541 ggaaagtaca gctgcctttg tcagtatgct gacggattgt aaagaagtat ctacagtatt
     3601 taattgatag ttgtaagtaa gatgaatggt atctcctatt ttgctcaata gatctgctgg
     3661 gacagaaact ccttgttgaa tgccggtatc tgcgatagtt gtaccattta attttaagga
     3721 accattgata taagtagtat tttcaggaag tgagatcgtg atttttttgt cggttatcgc
     3781 atccccagat tttgttaacg tgaatgtcac attttcttga atcgtatctc ctgatagagc
     3841 agacagggga tctcctgtct caggttgtct gctaaggttt tttagctgac tatctgcact
     3901 cacagatgga aaattagcca ctgttatttg aggaagagtg gtgattccag cttgttcaga
     3961 ctgccatttg tacgcaaaag acggatacat gttagtaagt acatcgtcca ccaagcgata
     4021 atcaaaataa tattgccctg aagccaatgc aggtagtgta gaagtagctg tatttacacc
     4081 cccgttattt gattggatcg tttggaaaag ttgatctgtc catcctgtcg ctaatttttg
     4141 ggttcctttc gaataaagcc tgtaatggat cgaacccttt tttagttcag ttgctgacca
     4201 attggccgtg acttggtacg atgcagaaga aaggagcttt ccccactgac tatcggctgt
     4261 taagtttgtt ggtaaaaccg ttccgtctag cttgtactgc tccaatacct ttaaagcaac
     4321 aggagaaagc aatccgcctt gcgcatcttt cccactgtac acgatattgg ctgtctgatt
     4381 caatccactt gtgcttgctg gagtatgagt cagtattgct ccttgtgtaa cgttttttcc
     4441 tgtaaaatag ttgactacgc gatcaaagcg actaaatggc aactgatcaa aacgtccttc
     4501 agtcagcccc atcgtcaatg tccgactatt ggtagagcct ggaaggttct ggttatttcc
     4561 atccacgtat tctgtggtat cagcaaatgt attatatttt cctgttgaat cacttggtga
     4621 gatgtaacta ttccacgtat ttatctctgc acttgcagaa aatgcatatg ctcctggatt
     4681 caagtttccg ccagttaatt taggataaaa tctacctgca gctggagcta aaatcgaatt
     4741 gaacatacga tcttggataa tattattcga atcataaaaa tcaacagaaa aaacggcctt
     4801 tttagacata gaaggttgta atttaccctt ggtcaaaatt ttgtttggac tcgtatcata
     4861 tttatctccg ttttctgtca tcttcgcgac attagctgta acatcaaatt gaagcgggat
     4921 atcgtccacg ttgaattcgc ctcgcgtaat aatagcaata atatctgtca atagagtatt
     4981 tgtatcccac gaccccatcc caaaacgcaa ggtatggctt cctgctgata ataaacttcc
     5041 gctagtaacc ttgatggggg caccggcaat atacaagtag gcgttgttcc agtcaattgc
     5101 ttttaataca tcactgcttg atacatgttc cggcaaattg atgatcgcca caaatgcaaa
     5161 tccttgtgca gtgatattca ccaaatcaag tacatttgtt agtttcacat ggctttccca
     5221 atgaaaacgg accgtgccac ctttccaagg atcatttgaa ccatcgttca aataagtagc
     5281 agtcaattta ggcaattggt tagccccagt tagttggaca agcttcccgc cattgactgc
     5341 actacgtcca ccatttggag gtacgatatt ttcatacgtg tatggatcat aagaaatggc
     5401 aggaggcgtt gctccaacag cttctgccga aggtgaaaag ataaatatac ttaaaacaaa
     5461 aagcgaagta aaaaagaagg agcgaaaaaa tgaaaattta ttttgtagca acatgttcat
     5521 cctccttttc ctttcttctc cataacaaca accacgcaag aagccataat tctataccaa
     5581 tgatcaaata aagaaaacgg atcttttgtt cattcgtctc cgggaatacg ccatgttgat
     5641 tagattcttt ataaatcggt agctgtgtct gctgttttga tgcgccattg actgaaccac
     5701 ttggatgcgg aattggactt agatcataaa atgtaatcgt acctgaactt ttggtatctg
     5761 cttggactgg ctcttgccag ctgaagaaca gcaaacttgc aataatccct cccaataaga
     5821 tcgatgtttt caacgaaatc acctcatcat gttgtttctc cttttttctt tttcttgtgg
     5881 acccacaaaa acagcaaaat caaccataac actagaactg cagcaaccaa tcctgcataa
     5941 agccatggga aagattgttc ttgtactgca aaaacggaat tttcatttac tttttgtgct
     6001 ttttcttttt tgatcgtaaa gttttgtgta aatcgccatt cattactgtc gcctgatttt
     6061 gctatgattt ctgcgcggta atcgccggct tttgctttat caccagctaa atcaagattc
     6121 aagtgaaaat tcgaatttgg tgcaactaaa aatgcattag acgtattttc aatataaagc
     6181 tgattttcat aaaagatctt gctcgtaaat atcaaattgc tgataatcct aggctgagga
     6241 ttttggaaac gaagctgaac agttggcttt gcgttgacct cgtccaaatc tcctgataaa
     6301 agtttcagat ccggttctat cgtctcttga ctttcttgta aaacgactgc gagattataa
     6361 gcaatacgat ttttgatttg tgctccttct gtagcatttt ctgtttcagc ttcttcttta
     6421 ggtgaaacat gaatacccgc taatacacgt cccttaaagc tttttgctgg gattttcact
     6481 tctataggta cagttgtttc tgcatgtgct gatacattga tttccttttt cgcaatgagc
     6541 aaaacgtcac ggacatcaaa cggcaatgtc tcatctaacg ttgggttttt ttgactataa
     6601 tcgatcgttc caccatcact cgttaccgcc ggattgacag agacctcaaa tgttcgttct
     6661 tcatctgaag aattgctgat aattacttct agtgtctgat tttgttccgg tgccactaat
     6721 aaatcaaaat aaccgattgc tttatctatt tggttctctg gaaataccgg tttgattcca
     6781 aagtctcctg ctgaatcctt tgcttggctg attgacggaa aaatgaaaaa tagacttaaa
     6841 atagaaaaaa acgaaacaaa cagtcgttta ctttgattca ttgtccacca catcctttca
     6901 acaaaaactg cggtaaaaaa tccttcacct atagtatttt caccgcagtt tgttgaattt
     6961 atttcatact ctatgctact gggccagcca cgaggttcca agtcaaatca gccgtgaatg
     7021 ttccagctgc tgctttcggc actttcagtg tgatatttgc tggatcataa acagaaagcc
     7081 atgttcccag accttgatct tttgctgctg taaatattgg tgtcgctgca cttgctttac
     7141 tcaacccagt tacagtttga ctagttggtc cagtggattc gttatctgac gtacttttaa
     7201 cagtggatgt tccaaaatct aattcagcgc cttgcaaaac ttgtgaaccg tttacaaaat
     7261 ctgaaaccga aactgtcacg ttccaccctt gtgcttggcc atctgcacct acacctcgac
     7321 gatctgatac ttggagataa ggtgtatcgt tttttgaagc gtctacttga tacgtttttg
     7381 ttccgctttc aatctcgtgt gtaccaaatg gcaattcggg aactacatcc agtgttaaag
     7441 gacctgtctg gccagttgct ggattttccg gatctgctgg ctctactggt tttgaaggat
     7501 cagtagggtt aagaggctcc acggtatttg ttggttcagt aaaggtaata tctccttttg
     7561 aagtcgcttg accctcagca aaagctggtg ttgccaagcc gcctagaaga agtgttgaaa
     7621 gtaataatgt cccaatcatg ttctttttca taataaatca ctcctttttc ttagtgacct
     7681 aagtatagta gagacagtat gcaaaaaatc ctcatgaatt catccgctta cttgaaatta
     7741 tgaccctttt tgtataaaag agaaaaaaac aataaaatcg tttacatgaa tattcttcct
     7801 tttttattaa gtatgaaaaa atatttcaat tttatttttt cgtttcaaat tattctatta
     7861 gtgacatcct tgaactataa aaataggagt acggcatcat ctgccgcact cctatttttt
     7921 tcgtcatttg tttcttaaat aaaacgattc tcacgcaatt ttattttttt gatatcttct
     7981 actgtttgtg ttccagctaa ttgcatgacc atttccaatt ctgttttaaa gaaatcaaac
     8041 acttgatgga ctcctgtact tcctcctaaa gacaatccat aaataacggg tctgccaata
     8101 gcaacaagat ctgcacctga tgcgattgct ttgaaaacat gctgtccgcg tcgtacaccg
     8161 ctgtcaaaca cgattggtac acgtttatct actgcttctg ccacatattg caacgaatca
     8221 aacgctgctg gaccaccatc tagttgacgg ccaccatgat tagataccca gattccgcca
     8281 gcaccagatt ctaatgaacg atagacatct tcttctgact ggacaccttt cacatagact
     8341 ggcaaatcag aatgagcagc aataaattct acatcttttg gactaagctt ctgtttggaa
     8401 gatttgtaca cagcatccat cgtttggcct actcctgatt gataagcctg aacgatcggc
     8461 attggcagtg ggaaggtaaa cccatttctg cggtctgttt cacggtttcc gccaactgtt
     8521 gcatccgctg tcaatacgat tgcttttgct ccattacgct tggccacttc aagaatatcc
     8581 aaattgatcc catcatcttt gctcatataa aattgaaacc attgcggtgc tttctccccg
     8641 cctgcttctc ttatctcttc taatgtacaa gaagcgtaag aacttgctgt atagatcgtc
     8701 ccaaaatctg ctacaccttt tgcagaagct ttctccgcct tcacatgagc taagccatga
     8761 gcagcaactg gtgccatgat gattggtgca gtcagcgttt ctttgtcaaa atgtgtagtt
     8821 gtatcgggta attctacatc ttttaataca tgaggaataa tcagctgatg attgaatgcc
     8881 cgttcatttt cttgataagt aaataagtcg ccagcaccac tgctgatgta accataacca
     8941 ccagctggaa tcacttgtga agcggctatt tctaaatctt ccatattgat aaaatccact
     9001 attccttcat ttgtacctgc ttgatacgtt ttttccattt caatgccttc cctctataaa
     9061 aaattgaatg aataatatga tagcgtttac acactttata ttactcttcc gtattgatac
     9121 aggcaaataa tctgtttaca tttcagtcat tccagttgtt actactctga aagaaatgtt
     9181 tagaacacaa atagataaaa aacgatggga ctttgacaaa agtcgtgtca aagtcccttt
     9241 tacgaaatac tgtggatgcc tattgaaata agtgcgggtt gtttctttgt atttcaatcg
     9301 tttcttgcca ttcttcgttt aattttggat taccgttttc ttccttgaac gcataaggtc
     9361 ccaatccaaa aagctcagtt agtatttgat tcacgattcc atatagctcg gtgtgatcgg
     9421 acacatgatt attgaagatc atctcattat caccacgttt gaatctcaca ttcgggctct
     9481 catgatgaac ctctatgcag ttttcccctt tttttaactg acggatttct gaaaacatct
     9541 cataaatatc ttctccaata aaaccttcag caagcgccag actgtccaca agaccgccta
     9601 ctgtcaactc agagattatc gctttgttgg caccaagctt taatgtatag cttgttgctt
     9661 ctactacgcc gcatgcattg caaccacctt ctgatagttc gctttttctg atcaattgaa
     9721 cttctttcat tttctgttcc tcctcttagt tttctactta tgtatttttt atcacctgac
     9781 gtaagtgtat tttatcgtca cgatcacgtg atttattctt cagagattct atttatctat
     9841 ctaacatatc ctttattctt ctacttttct ttctttagca tataagagat acgcatagta
     9901 atcaagcaag gattgttgat ataaaaagaa caaataccgc tattttgatt tcttttgact
     9961 ctattactca tataaataaa atttattata cctgctcttt ttgactatat cctcatctga
    10021 ctcctatact tcgtttgtat taaaacttag gaggaatcat tcatgcttac agggctaaac
    10081 ttattaaata atccttttct aaataaagga acagctttta caaaagaaga acgggcaaaa
    10141 tacggaatca ctggtatgct tccaagtact gtccaaacat tggaacaaca atctgtccaa
    10201 gcttacggtc aatacttaag caaacagacc gatttggaaa aacgtatctt cttaatgaat
    10261 ctattcaata cgaaccggac attgttttat aaattgatgg gcgaacattt agttgaattc
    10321 atgcctgtcg tttatgatcc tgtcgttgcg gattcaatcg aacaatacaa cgaaattttc
    10381 ttgaaaccac aagatgctgc atttctttca atcgacgaac cagaaaatat caaagcaagt
    10441 ttgaaaaacg cagcagatgg acgcgacatt cgtttgatcg ttgtgacaga tgcagaagga
    10501 atcttaggta tgggtgactg gggtgtgaac ggtgttgaca tcgctatcgg taaattgatg
    10561 gtctacactg ctgcggctgg tatcaatcct gcacaagtct tgcctgtatc aatcgacgct
    10621 ggtactaata acgaagaatt attgaataat ccattatact tagggaatcg tcatgctcgt
    10681 gtagaaggcg aaacttacta cgaattcatt gatcaattcg ttcaatcagc tactgaatta
    10741 ttcccagaat tgttattaca ttgggaagat tttggacgag gaaatgctgc aacgatctta
    10801 gaaaaatacg aagataaaat cactactttc aatgatgata tccaaggcac aggaatcgtt
    10861 gtactagctg gtgtattagg tggattgaat atatcaggtg agcaattgaa agatcaaacg
    10921 atcttgacat ttggtgctgg tactgctggt gtaggtatcg caaacatcct tttagatgaa
    10981 atgatccgtc aaggtgtacc tgaagaagaa gcacgccaac atttctatca agtagataaa
    11041 cagggtttat tgtttgaaga tacaaaagga ttgactcctg gtcaaatccc attcgctcgt
    11101 aaacgctcag aattcactaa tgctgatgaa ttgacaaact tagaagcagt tgtaaaagca
    11161 atccatccaa cgatcatgat cggtacttct acccaacctg gagcattcac tgaagaaatc
    11221 gtgaaagaaa tggcggctca taccccacgt ccaatcatca tgcctttgtc taatccaaca
    11281 aaattggcag aagcaaaagc aaaagatttg attgaatgga cggacggaaa agctttagtt
    11341 ggaacaggta tccctgctgc tgatgtggaa tataacggtg taacttacca aatcggtcaa
    11401 gcaaataatg cgttgatgta cccaggttta gggcttggtt tgattgcttc aacagctaca
    11461 cgagtaaatg ctgaaatcat ttctcaagct agtcgagcat taggcggaat cgttgatgta
    11521 acaaaaccag gagcagctat cttgcctcca gtagcaaaaa tcactgaatt ctctcaaacg
    11581 attgctgaaa ctgtcgcaaa atcagttgtt gcacaaaaat tgaatcgtga agaaatcacg
    11641 gatatcaaag aagctgttga atctgctaaa tgggttcctg aatataaatc tttagaagat
    11701 taagattgtt atagtaaaag aacatacaga aactttgtat gacgtctaaa aagaaaaaag
    11761 cgactgcggc aaaacagcag ccagccaact actgctcttt tgccgcttct ctttggcttt
    11821 tttcatacga tacagaataa gaaaggatct gtaaaaatgg cacaaaaaac aacaaccgca
    11881 actgcagttg aggaaaagaa aaaagaaggg ttttggtcaa caaaaattac cggcgtaagt
    11941 ttgccccttt atttgatcat ggtccttgtc ctgatcattg caatggcttt aggaaaatta
    12001 ccgacgaata tgattggccc aatcgctgtt ttggtcattt tcggtaattt atttcatttt
    12061 atcggaacaa aaatccctat cgtcaaaagt tatttaggtg gtggctccgt ttttgcgatc
    12121 tttgcctctg ccgcgattgc tacatttggg atcttacctg agtatgtagt aaaatccaca
    12181 gaaaactttg tcagcaatat gggcttttta gacttttata ttgctgcctt gattactggt
    12241 agcatccttg gtatgaaccg taatctattg atgaaagctt ctgttcgttt tattcccgtt
    12301 gcacttattt caatggtcgt ctcctttttt gccgtaggac tcgtaggtat gcttatcgga
    12361 aatggttttg caaattccgt tttatatatc tcattgcccg caatggctgg tggtgttgga
    12421 gccggagctg tacctttatc aacgatttat gcaaatgttt taggtacgga cgcttcttct
    12481 atcatttccc gcctgattcc tgcgacagca atgacaaatg tcctagctat cattggtgca
    12541 gcgttagttg ctcgtgcggg acaatcaatg ccaaaattca acggtaatgg taaattaatg
    12601 gaaaaaggcg accttggcga tggaaaacct agaaatgtaa aaccttccat tccacaatta
    12661 ggtgtcggtt tgatggtatc attgactttc ttcatcttag gacaaatctg taactatttt
    12721 gttcctaaag tccatgctta cgcattcatg atcattatcg ttgttatctg taaaatcgca
    12781 aacctattac cagaatatta cgaagatgca gctatcatgt tcaacaactt gattgtcaaa
    12841 aacttgacag cagctgttct tgccggtatc ggtattgcgt tattgaatct aaatgtattg
    12901 gcttctgctt tgacatggca atttgttgtc ttatgtttga caagtgttat cgcgatttca
    12961 gtagtttcag gatttgtcgg tcgtttgttt ggtttatatc caatcgaatc aactattact
    13021 gctggcttat gcaacaactc gatgggtggt acaggaaatg tagctgtctt atctgcagct
    13081 aatcggatgg aacttattgc ttttgctcaa atgggcaatc ggcttggtgg tgccatcgtt
    13141 ttgatcatct caggtttctt gatgcaatta ctttcttagt caaaaaaaat acctgatcat
    13201 caagtatcgg cttgatgacc aggtattttt tgataaaaat ttacaggatt ttcaaaaatc
    13261 ttttttgcat tttatcggaa tgataaccga tgcgttcata aaatcggtgt gcttctactc
    13321 tgtattctgc agaattaagt cttattgctg atattcctcg tcttttcgct tcttctacgc
    13381 cagtcatcaa ggcctttccg attcctcttt tttcagcatg tttagacaca gcaagtgcca
    13441 taatattgaa catcggatcg ctgaaaatcg attcatagga ctccgcttgt acatagccag
    13501 ctgctttttt agaagtagga tcttcataga ccaagaaaaa atttttttct ggctccttca
    13561 acagtttctt catttgtctc accgtaacgg ttaatggtac atcatatcct aattgttccg
    13621 cattgatttc tcgtagtgct tctgcatctc ctaaacgcat ttcccgcaac atccaaatca
    13681 ccagccaatt tttatttttt taaatgaatt tttttatcac agtatactcc ttttttcatt
    13741 tagaacaata tgtttcagtg attttgactt gcgttagtct agattctcct aatgtttgta
    13801 agatgacaaa ggcattgtaa caattgacca aaagaaccac gaggacaatt acttgaaaaa
    13861 taaaggtcac tttttttgga gacaaaaccc tgctaaatct tgctccccac aaacctccaa
    13921 taacagcagc ggggataaca taaaacaaca tagataaatc aaagcgcata aagctacttg
    13981 ttaaagcaat cgttaccaat tttgccagtt gagaaaagaa aatcgtagat agagaatata
    14041 gtgttgcttc ttttattggc aatgcaaaca tcagcatcaa caaagaaaca ttgactggtc
    14101 caccaccaat acctaaaaaa ctagctaaaa agcccagaac ccccccacat ataaggtacc
    14161 aagtccatga cgtcaaatga aattttggct gatgatgctt ggtgtagaag aacgcaaaaa
    14221 ccaaggtcaa taccgtcaaa aaaatttgga tcagctgcac atgcttctcg ttttcaaata
    14281 actgtaaaaa aacttcgaat acaatgttgc cgactactcc tccaacaaca gctccactag
    14341 atacccaacc gacaatctgc caattcagtg atttcccgct tgatacttgt cgcacagtcg
    14401 aaacaatgga catcgtgaat accgcaactg ttgagtaaaa agaaatccct gcaacagaat
    14461 gggcgccgat aagatcaaga atcggtttga tcaacacgcc tccacccatc ccggaaatag
    14521 cgccaactgt gttcgccaga acgatcacca caaaataaat cagaccaatc atcttgtatt
    14581 tcctttctct tgtttatatc ttttagcgat tgttactctt ttcttttatt atacagtaaa
    14641 agaaacaaac gaggcttcga aagcttttct actttttaag gaaatttagt gctaaacgaa
    14701 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagct
    14761 cctcataatt ttcgtttggt tgaacttatt accgcagatg caagcttttg aacaccgttt
    14821 aagaaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttcaaaaaac
    14881 agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttggcag gagaacaccg
    14941 tttaaggaag agactgtgaa gtcaacgttt tgacctgatt attagagaaa caaatggcaa
    15001 aatagttctt catatttcgc cgtttttcga gcgaatgccg aggggcagct gattgaacat
    15061 cgtttatttg gaaaaaggga ttgtgacaag acgtttgtca caatcccttt ttttatatct
    15121 tattaattca ttcttttgtt ctattcttct tcttccgcca tgaatgagaa acccatatca
    15181 aagaactata aaggttatca aaacagcgtt tatgactaaa tgaattagag ttatctatca
    15241 ctatttttca agcgatttaa accaaatggc atacaatatg gcatacaaaa acttcaataa
    15301 gccgtggggg tttttaaatt tcttcttgtt ctttaaaatc aatgagtgga gctacataat
    15361 ttgaatccac tttttcaaac gtcacttttt gcatcgacga atgttttctt tcccaagata
    15421 acgtaaatag atttgactct ggacttataa agtctacttc accttctagt att
//
LOCUS       DAZKOR010000065        15416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000065 DAZKOR010000000
VERSION     DAZKOR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.066"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(198..1046)
                     /locus_tag="ACG8ZM_001949"
     CDS             complement(198..1046)
                     /locus_tag="ACG8ZM_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0492896.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1210..2112
                     /gene="gnd"
                     /locus_tag="ACG8ZM_001950"
     CDS             1210..2112
                     /gene="gnd"
                     /locus_tag="ACG8ZM_001950"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0492897.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2114..3115
                     /locus_tag="ACG8ZM_001951"
     CDS             2114..3115
                     /locus_tag="ACG8ZM_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0492898.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3112..3396
                     /locus_tag="ACG8ZM_001952"
     CDS             3112..3396
                     /locus_tag="ACG8ZM_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0492899.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3429..4226
                     /locus_tag="ACG8ZM_001953"
     CDS             3429..4226
                     /locus_tag="ACG8ZM_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0492900.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4240..5073
                     /locus_tag="ACG8ZM_001954"
     CDS             4240..5073
                     /locus_tag="ACG8ZM_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0492901.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5091..5948
                     /locus_tag="ACG8ZM_001955"
     CDS             5091..5948
                     /locus_tag="ACG8ZM_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0492902.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5970..6479
                     /locus_tag="ACG8ZM_001956"
     CDS             5970..6479
                     /locus_tag="ACG8ZM_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0492903.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6516..6917
                     /locus_tag="ACG8ZM_001957"
     CDS             6516..6917
                     /locus_tag="ACG8ZM_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492904.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7029..7382
                     /locus_tag="ACG8ZM_001958"
     CDS             7029..7382
                     /locus_tag="ACG8ZM_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492905.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7416..7859
                     /locus_tag="ACG8ZM_001959"
     CDS             7416..7859
                     /locus_tag="ACG8ZM_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0492906.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7876..8961
                     /gene="uxuA"
                     /locus_tag="ACG8ZM_001960"
     CDS             7876..8961
                     /gene="uxuA"
                     /locus_tag="ACG8ZM_001960"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0492907.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8954..9667
                     /locus_tag="ACG8ZM_001961"
     CDS             8954..9667
                     /locus_tag="ACG8ZM_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0492908.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9874..10023)
                     /locus_tag="ACG8ZM_001962"
     CDS             complement(9874..10023)
                     /locus_tag="ACG8ZM_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492909.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10419..10619
                     /locus_tag="ACG8ZM_001963"
     CDS             10419..10619
                     /locus_tag="ACG8ZM_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0492910.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            11187..11750
                     /locus_tag="ACG8ZM_001964"
     CDS             11187..11750
                     /locus_tag="ACG8ZM_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492911.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(11811..12176)
                     /locus_tag="ACG8ZM_001965"
     CDS             complement(11811..12176)
                     /locus_tag="ACG8ZM_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0492912.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12252..12497)
                     /locus_tag="ACG8ZM_001966"
     CDS             complement(12252..12497)
                     /locus_tag="ACG8ZM_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0492913.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(12567..12821)
                     /locus_tag="ACG8ZM_001967"
     CDS             complement(12567..12821)
                     /locus_tag="ACG8ZM_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492914.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(12985..13308)
                     /locus_tag="ACG8ZM_001968"
     CDS             complement(12985..13308)
                     /locus_tag="ACG8ZM_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492915.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            13495..13568
                     /locus_tag="ACG8ZM_001969"
     tRNA            13495..13568
                     /locus_tag="ACG8ZM_001969"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13529..13531,aa:Ile,seq:cat)
     gene            complement(13953..14450)
                     /locus_tag="ACG8ZM_001970"
     CDS             complement(13953..14450)
                     /locus_tag="ACG8ZM_001970"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0492916.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(14490..15299)
                     /locus_tag="ACG8ZM_001971"
     CDS             complement(14490..15299)
                     /locus_tag="ACG8ZM_001971"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0492917.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT         5170 a         2486 c         3147 g         4613 t
ORIGIN      
        1 tactaacttt gttatcgcat ctaacaagta actcgaataa taccaataca accttctcca
       61 aagatgcgat gacatacgtt ctaacccacc gactattgat gttctgtacc acccttgcag
      121 aaccaaaaaa gccactacca aatatttggt agtggctttt tttgttttat ggatttttcg
      181 ctttttctgt tatggtgcta ttctttgatt tcagaaatag tatgtcgaac tttagcgata
      241 gaacgacgtg attctttttc gttgtaaaac atcaaaatca catatagaga gtccaaaatg
      301 cttagttgag aaatacggga agctaaagct tcagatcgga attctgtttc ttcagaaata
      361 gaaataaaaa cgacatcgcc aagtttagca agggaagaat tagcttggct agtaataacg
      421 attgtttttg ctccagtttt tcttacggtt tcagcaatat ggattgtttc ttttgattgc
      481 cctgtgtgag aaatcaaaac agcacagtct ttttccgtca ataaagaagc ttccatcaat
      541 tgaatatggt aatccgtgct gtgagaagtt gcgatagggg aacgcaaaaa tttatgataa
      601 gcgtcagttg ctacaatctc tgaaccgcca acaccgaaaa aatagacacg tttagattca
      661 ttgataatag tagccgcttt ttttagatcc ttttgttgta acaacttttt tgtgtcattt
      721 aaagttgaaa tactagactc gaacaccttt tgcgccatag tgagctcagt atctgttttc
      781 tgaatatttt cgtggataga aatagcagat agatcatttt cttggatcaa catggccatt
      841 ttaaaatctt taaatccatt ataaccaagt ttttttgtaa attgaaaaaa agtagagtca
      901 gcaatgccca gttcacttgc taaattgcta ataggactat gacttacttt gcttggattt
      961 tctaaaatat aatcggcgat agcctgttct tttgggctga agtcgttgta cattgttttg
     1021 atttgtagtc ttatggagac gctcatgaga attcctcatt ttcataataa gattatcatt
     1081 ttttccgttc tcattaatgt taaagataca aggagagtag gaaaaaatca aacaaaaaat
     1141 atttttgtaa aatctattta caaaaaaatt tttttgtata atgagggtat aagaaatgga
     1201 ggcgctatca tggaaattgg tgtaattggt ttgggaaaaa tgggattgaa tttagcttta
     1261 aacttaaaag agcaccatca caaagtacaa ggactcgaca tttcaagtac agctgttagt
     1321 caggcaagag aaaatgacat tcctgcttat caagagatct ctgaatttct agctactttt
     1381 gagcagaaaa aaatcatctg gttaatgctt cctgctggag agataaccga aaacatgtta
     1441 agaaaacttt ttccaaaact cggaccagga gacattatta ttgaaggcgg aaactcttat
     1501 tataaagatt caatccgtcg agcagaagag tttcagaaaa atgaaatcgg ttacttggat
     1561 tgtgggactt ctggaggaat cgaaggagca cgacatcaag cctgcttaat gattggagga
     1621 gataaagaaa cttttctatc cgtggaacaa ttattcaaag atgtagcaat tgaaagagga
     1681 tatctgtatg ctggtgcatc cggaagcgga cacttcttga aaatgatcca taatggaatc
     1741 gaatatggaa tgatgcaagc aatcggtgaa ggattccaac ttgtacaaaa aagtgagtat
     1801 gcttttgact tggaacaagt cgcacgtgtt tggaatcatg gttcggtgat tcgcggctgg
     1861 ctgatggaaa tcatggaaga tcagttaaac gaacatccca atttaagtaa ttatcgggga
     1921 atcgtttctg catcaggtga agcaaaatgg acggttgaaa cagcgctaga aatggctgtt
     1981 ccagttccag taattgcaac tagtttgttc atgaggaatc tttcacagga agaagatagt
     2041 ttttcagcaa aagtagtttc agcactaaga aatgggtttg gcggacatga aatagttgaa
     2101 aagggtgagt aaaatgccag attatcatat agcaatcgtt aattccagca gtttcggaag
     2161 agtctttcca caccacttgg aaagattaaa gtcagttgga gacgttcatt tctttactgt
     2221 tgatcaagag attccaggaa aagaactagc ggagttactt aaaggattca atatcattat
     2281 tgcaagtgtt acgccatttt ttcgacgaga tttttttgaa aacaaagatg aactgctatt
     2341 gatttcccga catggtattg gttacaacaa tattgattta aaagcagcaa aagaacacca
     2401 gacaatcgta tctatcatac ctgctttaat tgaacgaaat gctgtagcag aaaataatat
     2461 aactaatttg ttgtccatgc ttcgacgcac gacacaagca caagcaagag tcaaggacga
     2521 tcgatgggaa gatcgggcgg agttcgtcgg taggacgctg tttaacaaaa cagttggtgt
     2581 gattggtgca ggaaataccg gaagcggagt aatcgaaatt ttgcgtaacg gttttcgctg
     2641 tgatgttcta gcttatgatc cttacaaatc agatttatac atcgaaagct ttggtgcaaa
     2701 aaaagtagaa cttcaagaat tactggaaca atcagatatc atttgtttat gtgcgaattt
     2761 gacagaagag aactatcaca tgatttctga taaagaagtc gatcagatga aagaaaatgt
     2821 ttatctatct aatagtgcaa gaggcgctct agttgatgaa gaagcgatga ttaatggtct
     2881 gaaaagcgga aagatcgcag gatatgccac agatgtttta gaagaagaac cgggacgtca
     2941 agatcatccg tatttagcgt ttgaacaagt agtgattacg ccacatatat cggcctacac
     3001 aagggaatgt ttagaagcaa tgggtgaaaa atgtgtcaga gatgtcgaac agatcagtca
     3061 gcaccttctt cctgaacgtt ctgtccagac aaagagcaac tatataaaag gatgatgaag
     3121 aaaatggtga acggtttaaa agtgaaaaca ggccctcaat tttatttata tgaagaaggt
     3181 ggtatttcaa aagtttctga tctcttgaaa tcatacggcg cgaaaagagt actggtcacc
     3241 cacggaacgg tatcatggga aaaggcgcta cctaaattag tatttttgaa tgacgaaacg
     3301 attcaatttt tttatcatcg ttattctgga gaatgtagtt atgctgaagc cagacgaata
     3361 gctacaatca tcaaaaaaat gaaatcgatt tcgtagttgg cgttggcgga ggaaaactga
     3421 ccgatctagt gggttatagt gccaatcttg caagaatcga tttcggagtc atccctactt
     3481 tagctagtaa ttgtgctcca tggacacctc ttgctgtcat gtataaggag aatggacaag
     3541 cagaagggaa aacagaacac ttttacagac aagctgcttt ttttataaca gatccccaat
     3601 tagtgattga ttcccctgtt ccatatttca ttgccgggtt agcagataca ctagcaaaat
     3661 ggtatgaatc tgagctcatc ttagaacaag attttttgca agatgaatca tttttgaagc
     3721 tagcacaaga tactgcgaag atctgcaaag aagagatttt gaatcattct gctaaagcaa
     3781 tccaagatat ggcacaaaga aaactgacat cggagttcaa acagctttca gagattatct
     3841 ttgctgttgc tggtttagtt ggaggattag gagataaata cgcaagaaat gcggcagcac
     3901 atgctatgca tgacgctatc agtaaatata ttccggaaag ccatcgtttt ctacatggag
     3961 aaaaagtagc gtatgggatt ttctatcaac tggcactaga aggtcgctgg gaaaaaatag
     4021 acgagttata tcctttgtat cgggaattag aactgccggt ttctctagaa caaatgggat
     4081 tatttccgaa agataaacag ataatagata aaatggctga atttattgat tcgaaagaga
     4141 aagtgcattt gattcctata gaggtaacac agaaatcact gatacagtca atgatggaac
     4201 tggaaggtta tatcagaaag atctgaagga ggaggacaca tggaaacatt aagtgtagta
     4261 caaagtttgt tgattgcttt atgggtagcc gcggttatgt cccgctggtt aggtggagga
     4321 gccacgttga ccttacgttt ttctcctttg atgactggat tagtcgtcgg gttgatcatg
     4381 ggggaagttg cacaagcgat gattgttaca gccgctttgc aaatgattta tatgggggtt
     4441 ttttctccag gaggatcaat gccagctgaa ccatctattg cagcagctat cgcagtacca
     4501 gtcgcattat taggggatct gtcgccagaa gcagcgattg ccgttgcggt gccagtcgga
     4561 ttgctcggaa gttacttgta tcagtttcgt tttttcatca atactttttt aggtaaatat
     4621 acagacaaag ctgtagaaga tttgaataca aaagctatgg cccgatcaat tattctttat
     4681 ccaacggttg cctcattttt attattcgtt ccgcttgttt tcattgcttt atatttaggg
     4741 gcgccattga tcgctgatgt aatcaaagct ttagagggaa cagtcgttat ccacattctt
     4801 gaagtagtag gtggagggtt agcggcaatc ggtatcgcga caactgtata cgttatcggt
     4861 cgaaaagatt atctggtctt tttcttcctt gcttatttta tgagtgttgt tttgaaatct
     4921 ttagatatta cgatggttac ttatgccatt tttgggatta ttatcgcttt gatctttgtc
     4981 cagtcgcaaa aagggaaaca agtacagcct gccacaagca ataacaattc agcatctttt
     5041 gatgacgaag atgactatga tgacggattt taatttagga ggaatgggaa atggctgaaa
     5101 taatggataa tcaaacaggt gctcctgaag aaatcacgaa aaaagatgta acaaaagcct
     5161 atctgagatg gcattttgca aacgaaattc ctcactcttt tgaacgttac ttggcgccat
     5221 cactactata cggaatgatg ccaatcttga aaaaacttta taaagatgaa gactctttaa
     5281 aagcagcata taaacggcag ttactgttct tcaacactca attaagctgg ggtggcggtg
     5341 tgattactgg cctaatgtct tctatggaac agcaaagagc agaagaagag tatgaagaaa
     5401 aagagatctt gatgcaggat gacttgatgt ataacacgaa agcgggattg atgggggcac
     5461 tagcaggaat cggagatgca atcgattcag gaaccgtcca atatattttt atcgcaatcg
     5521 cggtaccttg ggcacaacag ggaagtcctt taggcgcact ttttcctttt atctgctttg
     5581 ggttgtatca ggttttacta ggggtatttt tcgcaagaca agctttcact atgggccgta
     5641 atgctacagg cctaatgcaa aacactggtg ttcagacagc gattgaaatg ctatcagttt
     5701 tagggttgtt tatgatgggg gtgcttgcgg gaaactatgt aaaagtagaa tcttcattga
     5761 aatttgcgat atccggacgt gagtttgtta tccaggaaat tcttgatcaa atcgtgccag
     5821 ggatattacc tttagctgtc gtaatgggtg tgtacttgtt ctatacgaaa aaaggattga
     5881 aagtcacaca agcattatta tggctgactg gaatattaat tgtgctagca ggaatcggta
     5941 ttttataaaa agaagggaac gtgaataaga tgggtgtagt caatttagcg cgtgtggatg
     6001 agcgtttgat ccatgggcaa gtaatggtaa cgttatctca aaaaagtgga gtgaattcca
     6061 tttttgttgt agacgaagtt gtagcaaaag ataaatttat gagagatctt tacaaaaatg
     6121 caggaagccg tacaggacaa aaaacaattg tcattacacc agagaaagct aaattctatt
     6181 gggatgaata tcaatttaaa gaatacaatt gtattttgat cgcaaaaaca gtccaaacga
     6241 tttacgactt agtaaaacac ggaatcccca tggaagaatt gaatattgga gggattgctc
     6301 aaaaaaatcc tgatacagat cttttagtga cgaaatctgt ttatttaaat aaatcagatg
     6361 ctgaaaaatt gaaagaaatg aatgaacaat atggcgtaaa gaatatttat tttcaagcca
     6421 ctccatctgc acctaaaaca ggattaaaag atgtattgaa caagtttgat ttgtcataaa
     6481 caaatgctta gaaaaaagat aaagagggcg aaaaaatgaa gaagaacgag ctgtttcgtg
     6541 attgggaatt tcgttatcgc tatatttacc gtaagcgccg tacaaaaaag agtaaacagc
     6601 gttttctttc agcattagta tcagatattt acagtatgag aacagatgtg acagtcattg
     6661 cttacgatac gctagcctac cgatcaaaaa atatttatgt tggagatatc gaaaaagcag
     6721 agaaagttat ctgtacttat tacgatacac ccgttcatgc attaggaagt tatttcatgt
     6781 ttgattggaa agaccaaagg aaaaaaacaa tctactctat attattgtcc ttcattttgt
     6841 tgttcagttt aggttggtgg ggaatgatga tatataacaa aaatcctcat cacgtattcg
     6901 atctgctatc cgtataaaca ggtatcactg tactagcatt tgggagctac tttttcttat
     6961 tgggaaaagc agctcgcgga tggcctagca gacaaacttt tattcgaaat acttcatcca
     7021 tattaacgat gctagaaatg atccgtacga ttgatgatcc gagtgtagcg tatgcgtttg
     7081 ttgatgaagg ctgttatgga gaaaagggct tagacagcgt gagatcgggt atgaaaaaag
     7141 aggctattct tttttatcta gactctgttg gggcagatac accgctacag ttttcgggaa
     7201 attatttttc aaataaagaa cagtggctga aacaagttga taagttgaaa gaaaaaaacg
     7261 tcaattatat ttttagtgca agaaaaaagc aagcgcagtt cttctatttg accaaaacag
     7321 atttacgagg gaaaacattt aattggcaaa atgccaatca gattattgct ctttttcgat
     7381 aagtaaaaag tgcaaaacaa ggagtgagaa gactaatgtt ggaaatcatg atcgttacac
     7441 atggtaagtt gagcgaagga ctgaaagatg cggcggaagt aattgtcggg acatcgagcc
     7501 atattgaaac aatgtgctta gagtctgggg aggacatcca aacattcgga gagaaggtca
     7561 aagaaaaatt aactatgatc aacacagcag aacccatgct agtatttgtt gatctaatta
     7621 gcgcaagtcc gtacaatcaa gcgctcttgg cagtcaatga actgactgaa gaaaagaaag
     7681 cagccactta tgtcataggg ggcgtgaatc tgccaatgtt gttagaagca atcaatcatc
     7741 agatgcttca cacaccgata gaagaagcgg tggaacaaat tgtcaaacaa ggaaaagata
     7801 atttaactgt ttggcatgct tcagcggaag aaaaaaaatt agatgaggac gatttttaga
     7861 aggaaggctg aaaagatgaa gtggggattt agatggtatg gtgaaaaagg ggattcgatc
     7921 ccattaaaat atgttcgcca aataccggga ataaatggga taattggaac tttgctgact
     7981 aaaatgcctg gagatgtttg ggagatttca gaaattgagg agttgaaagc atctgttgag
     8041 agccaaggac tggaactgct tggaattgaa agtgtagcga tccacgatgc aatcaaggca
     8101 gggacagatg aacgagatta ctatattgat aactatatac gaacaatcaa aaacttgtct
     8161 cgatgtgggg ttcgattgat ttgttatagc ttcaaaccaa tttttggatg ggcgaaaact
     8221 gatttgttta atgaaaacaa cgatggaagc tattctcttt tatacgatca gaaaatcgtc
     8281 gatcagatgg agccaggaga aatgtatcaa ttgatacaca gccagtcaaa gggattcaaa
     8341 ttgccgggtt gggaagaaga acggttgaaa aaattcgctc agctggaaaa actgtatgaa
     8401 ggaatgaccg aagaagaatt atttgaaaat ctgacttatt ttttagaaaa agtgattcca
     8461 gtgtgcgaaa aagcagatgt aaaaatggct atccatccag atgatccgcc atgggaactt
     8521 tttggttttc ctagagtgac gaaaaatctt ggcgacttga agaaaattct aaatgcagta
     8581 gattctccat ataatggact aacgctgtgc acaggatctt taggggcaaa tccggaaaac
     8641 gatttagttg aaatcatcca tgaggtaggt tcgcgcatcc attttgtcca cttcagaaac
     8701 gtggagtttt taggagaaag aaagttcaaa gaaacggcac atccctctac agaaggctcc
     8761 cttgatatgt atcatattat gaaagcgttg gtgcaagaag ggttcgacgg tcctatacgc
     8821 cctgatcatg gtagaaccgt gtggggtgaa gtggctatgc ctggatatgg cctgtatgat
     8881 cgtgcaatgg gactcactta catgcaaggt ctatatgagg cgataagtaa ggaaaaacca
     8941 ctgggagagc attatgaata aaatcaagct cttcatcttt gatatggatg gccttttatt
     9001 agaaactgga cgtttagctt atcgtgctta tgtaaaatca gcacaaaagt atgattatga
     9061 aatgcgtaag gaagtctatt atttactaac tggccaaaca gaaatggcga taagagaaca
     9121 aatgggcatt ttgtacggag aagatgttcc atatatcaaa tggcgagaag caatcaatca
     9181 atataaagaa aaaatcgtga aagaagataa gagagtatat acgaaaaaag gagcagaaga
     9241 aattttatct tttgcaaaag aaagaggaat ccatacaatc gtggcttcgt ctaatacacg
     9301 ggtaaaagta gaaatgtatt taaaaatgga aaatctatat gccttgtttg atcaaatcat
     9361 ttcaggggat gatgtaaaaa aaggaaagcc ggaaccagag atctttttga aagcttgttc
     9421 aaaaatgaat attcctccaa gtgaagcact tgtatttgaa gactcgattg cagggatcga
     9481 agcagcaagg cgagcgggca ttttgtcttt tctcatcaaa gatcatttga tagggttgcc
     9541 cgatcataat gggaagcata agttgaaagt agatgtaagt ttatatgaat ccaataaagc
     9601 agactatgaa ttttatgacc tgcatcaagc aaaacgattt ttagaaagta aagggttgta
     9661 tctctaataa agaggactat tttctagtcc tctttattta ttgtcctgca cgactgaggg
     9721 aacagatgaa aaagaagtaa ttttttattt tgaagtatct accttatttg tagtgaacaa
     9781 tttagaacaa acacaaaacg catctactgt tatctaccag tagatgcgtt taaagagaag
     9841 aacgagaatc gtccatatga gggtttccac tccttatgca gattttttta attcttgcac
     9901 tgcttgttcg atcgtaacac ctgtcgctac atgattggta ttttctttgt caaaaacgac
     9961 aaatagattc aaatctgtat ccaaggttac tcgatattgt atatctttgt tatagaatgt
    10021 catagccatt ctcctttctt taaatacttg ctgaaaggca acgaacgcgt tgataacgaa
    10081 attccattta taagtatacc accaattgaa aggttttcat agttaagaaa ttttaaagtc
    10141 aaaagttgca gataaaagca aataaacaag aaaacaaaaa gtgaatcaat cgcggttatt
    10201 caactttttc ttcgattcga agacaataga agacaatgaa ttttatattt cattgcatta
    10261 tccttgtgtt attagaaaaa gcatgatata ttaaatgagt aatctatttt gaaacgtaat
    10321 ttgagcgata tatctacata gataaaactc cttttgccta tcaaatattt attgcaacga
    10381 aaaaacgtac attgatacgt atcaggagga atttatttat gaataacggt acagtaaaat
    10441 ggttcaacgc agacaaaggt tttggcttta tcactggtga agatggaaat gatgtatttg
    10501 cacatttttc agctatccaa ggtgacggct tcaaaacttt agaagaaggc caagcagtgt
    10561 cttacgatgt tgaagatggt caacgtggcc ctcaagcaac aaacattgtt aaagcttaat
    10621 aaaaattgac ttttaaccgt cttttcgaaa gaaaagacgg tttttttgta cgaaaaaatt
    10681 cattggtcgt tgaatgggct gaatagtagt gtataaaaca attttgtgga gcgtgaagct
    10741 tttatggtag aggaaaatac gaataaagtg atgttgataa aaagattcct atcttgaata
    10801 tgaatcgaga tgtcttgaaa atgaatgagt ttgaccaatc attatcctat cgaaatttac
    10861 agtttcgttc gattggtctg tcaattaatg aaaaagtaaa aaagaatatg ttctaggaag
    10921 agggcggcac atctaaattt ttaagtaacg gacagttcaa atatcaagca ttgaaagagg
    10981 ctatcctact gtttgaagga atacgaatat ataaaaacga caaagaggaa aaagtagttc
    11041 attaaatccc ttcaagtgat gaaagaatga gatctaataa aacctgatct ttcatctgtt
    11101 tttttaataa cgtaataatt tttttatttt ttgtgctttc gccgttattg tagagtgatt
    11161 tagcattctt atttttaaaa ataactgtgt tatatattaa aaaaggcata atagttataa
    11221 gcttttttaa agaatattta gaaattatta attttcgtct gaaaacaaaa aaagaaagcg
    11281 ttattatttt agtctatttt caggggggga accttatgat aaaaaaacta tttttactat
    11341 caatattaac gctagcaatg gcaggttgtt ctgactctgg cttagcaact gaactgatca
    11401 caaaatcaac tgaagccacg acagaggagt taaaatgtgg aaacgatcac agtgactaca
    11461 gaggcttaaa tcgacgatgc atgggaatat taggcagaag caattgtaag catcgatgta
    11521 gtaatcgaag aaggtgtaga agaagcaaca gaaaaatcaa aagaaagatc aaaagaagca
    11581 atctgtgctg tgcatcgtat acagacgtcg aagatgcggt acttttaaac cacgtcagga
    11641 gatgttcatt tatgtctagg gaagttggcg atgaaccaaa actagagtac gcttttagtt
    11701 ttgcacgtga ggatgaagat aaccctaaaa cttacgacat tttagcttga tctaagatgg
    11761 ttaaagcttt ttaatgaagt aagaggagat agaaggcttt tctagttata ttaggtaaga
    11821 taaattcctt tttgctccaa atttgctaaa cattctgcta gataaacttc atcgtgttcc
    11881 ggataaatcg gcacgttgcg ctctacagta ggtaaatcaa aaaatgcttc gcatgatttg
    11941 ccacgataca taggattttt ccactctggt gctgggctgt aaccacgttt ttccatttca
    12001 tccatgacaa gctgatgata ttggaataat ctataaggag aatagtgaaa aacataatcc
    12061 acagtagcat gtttctttcc ccagccattt cctcttaatg cacagcattc ccgatgttgt
    12121 cctaaaagct gttgacgagg aagctttgaa atcaattttt catgccataa tcgcatagaa
    12181 atgtctcctt tatatataga ttgttaacct tagacgttaa atgaaaaaac cagcaccaag
    12241 gctggaaaaa atcacttcag tttgcttgct gtttctgagc gaccaattag gtaatcaacg
    12301 gaaatatcgt aaaagtcagc gatatagatt aattcaatga tgcttggaat ttcttctcct
    12361 tcttcgtact tacgatattt gaataaagga atacttaacg cgctagaaac agctacttga
    12421 gataaagatc ggccagttct taagaaattc agacgacaac gtaaagtatc cagtaaaata
    12481 gcttcttcga tactcattta atcacctttc attatagtag aatagttata atgactatag
    12541 aaagctacac atgcaatcaa tagtactcaa tgttttttat ttaaacgtat caaataaaga
    12601 atgacgatca ataaagacaa aaccagtcct gataattctt ctataaaaac actatcgggt
    12661 tcaaagtagt tgtaattaaa aactttccac ataaagtaga tagccggaag agtcaaaata
    12721 gctaaataaa accgttcttt gttccaattc ataagataaa caatacttgc gaccagtgcg
    12781 accgcaggca cccaaaacaa aactctcacc aacgtaatca tgtaacggac tcctcaacga
    12841 ttcatataat aaaagataac atgttattgc atacatcaca aatgaaatgc taggaaaata
    12901 aaagaagaat agatcaatga aaaatgaaaa agcagaacag ttttctgctg ataagcagga
    12961 actgttccgc tgaatgtgtc ttatctatcg agaaaaaatc aatggattga tatctaagta
    13021 attcaataca tataaaaaga ggacgatcgc attcactaaa aaggaaagaa tgatgatccc
    13081 atagcgaatg gaaacatatt taacaaatac ttttgtttga atacgggcat atctttttcc
    13141 tttatagctg agcatcatga caagaagcaa aagggtaatc acaccttgaa aaatcattcc
    13201 aataaacaga attcctgagg tttgataatt taattggaga aaataagctg ctgccgcaac
    13261 taaatcgaat aaaatcgcaa taaatggcaa aataatcact cctaacattg agaataaata
    13321 aaagaataac attttttcta aaataagcaa ataaaaatta aagtaacaaa atagttatga
    13381 cacaagtaaa agaaataaga ataacaatat tattttactt atattttgaa acttgtgaga
    13441 gtttgtcatt cgtgctatag ttgaaaagta acaaaaataa attttattta tgatgtacct
    13501 ttagctcagt tggttagagc agacggctca taaccgtccg gtcgtaggtt cgagtcctac
    13561 aaggtacata agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta
    13621 aaaacagctt cttatgtttt tacttggctg ttcttattac tgcagatgca agcttttgaa
    13681 taccgtttaa ggaagaggtt gtgaaatcag cgttttgacc tgagtattag cttaacaaac
    13741 ggaaaaatag ttcctcatat tttgccgttt gttaagctaa taccgagggt cagctgattg
    13801 aacaccattt aagaaagagg tcgtgagcct gccgtttcac tctgtgtcat aaaaaagcca
    13861 atctactttg atagaaaaag cacgtaggac taaaacagtt ctgcgtgctt tttttattgt
    13921 gtgttaaata tcgtaaatga agatagatat ttttatgatt cgacgataga gacgttgaaa
    13981 tctttcaaca gctctaaagc ggattctccg tgttttctac tggagactaa cattggatca
    14041 acaaaaaagt ctgcttcagg aaaagcactc tgcaggccta tcagattaga gagtacacac
    14101 aagtttgttt caatcccgac tacttcgatc tctttttgtt cttctttgct ttcaaataaa
    14161 gtttcctgga tttcagctaa agtttcaggt ggaatcgtaa agtaatactt tttaatcact
    14221 cgttcatttt cttgtggaga aagaagaggg atcaattgca aatcttctcg ttcttcttct
    14281 ttgtccttca gttcaactgg tatatcccgt gtgaagagaa tatattcatt attttggcgt
    14341 gctttagcta atctttgagc aatacgttct gccaattgat ctgactctgg aatataaaat
    14401 tcgctgtttg gatcaagaat atgattttgc atatcaataa taactaacat tttctttcct
    14461 cctacattca tatcgtcatt ttattctttt taatagaagt tttttaaaat ataagcaaca
    14521 ccagcttctt cattcgtcag tgttacttgg tttgcaattt cttttacttg tcgctgtgca
    14581 ttccccatcg caatactcaa cccgctgtaa ttcaacatag caatgtcgtt ttcttgatcg
    14641 ccaaaagcca tgatgtcact cgattcgatt ccaaggtaat ccgccagaaa aacaagtgaa
    14701 tgagctttag tgatacctgt aggcgtgatt tctaagaaat gcggatcaga gaatactgat
    14761 tggataccat ctgcttgcag atctctttgt agcttctgta gtttttcttc actcttatct
    14821 gttaatgcta atttaagata gctaagagaa ggatcttcca acatatttaa aatttgatca
    14881 aaagaatggt tctgtaaatt gaaatgttca taataagggt catctttgac tagatttgat
    14941 tcatcgaatg tcagctgatc atcttgggtt tctcggctag caatgatgga tattttcgct
    15001 tcttgagcat agaagaaggc ttttctcagt gtatcgctat ctatttttga acgtctgaga
    15061 actttcgcca tttttccatc cttcatttca gcaatcagtg aaccattgga agaaatcaaa
    15121 tagccaccga ttcctaattc ggaaaaaata tctttagcag aaatgctgtt ccttccggta
    15181 gcgacggtta cgattccgcc attttcatca aatttccgaa gtgcttggat attttcttct
    15241 ggtatctttc catttcgatc aagcaaggta ccgtctaaat caatcgctgc tagtttcatt
    15301 ttatcttcct actttctttt tacatatcta ttttcctgtt accttaagat taaatgagaa
    15361 tttaaaaaaa tgaaactaac ttgctttgtc attatgaatt ttttcaaaaa acattc
//
LOCUS       DAZKOR010000066        15139 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000066 DAZKOR010000000
VERSION     DAZKOR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.067"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACG8ZM_001972"
     CDS             4..2376
                     /locus_tag="ACG8ZM_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0492918.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACG8ZM_001973"
     CDS             2437..3897
                     /locus_tag="ACG8ZM_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0492919.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACG8ZM_001974"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACG8ZM_001974"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0492920.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="ACG8ZM_001975"
     CDS             6128..6277
                     /locus_tag="ACG8ZM_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492921.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="ACG8ZM_001976"
     CDS             6352..6945
                     /locus_tag="ACG8ZM_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0492922.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="ACG8ZM_001977"
     CDS             6958..7857
                     /locus_tag="ACG8ZM_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0492923.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="ACG8ZM_001978"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="ACG8ZM_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0492924.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="ACG8ZM_001979"
     CDS             8723..8983
                     /locus_tag="ACG8ZM_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492925.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="ACG8ZM_001980"
     CDS             9428..9634
                     /locus_tag="ACG8ZM_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0492926.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9655..9885
                     /locus_tag="ACG8ZM_001981"
     CDS             9655..9885
                     /locus_tag="ACG8ZM_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492927.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="ACG8ZM_001982"
     CDS             9901..10338
                     /locus_tag="ACG8ZM_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492928.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="ACG8ZM_001983"
     CDS             10331..11038
                     /locus_tag="ACG8ZM_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492929.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11202..11402
                     /locus_tag="ACG8ZM_001984"
     CDS             11202..11402
                     /locus_tag="ACG8ZM_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492930.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11418..11597
                     /locus_tag="ACG8ZM_001985"
     CDS             11418..11597
                     /locus_tag="ACG8ZM_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492931.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="ACG8ZM_001986"
     CDS             11922..12185
                     /locus_tag="ACG8ZM_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0492932.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="ACG8ZM_001987"
     CDS             12179..12529
                     /locus_tag="ACG8ZM_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0492933.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="ACG8ZM_001988"
     CDS             complement(12553..13167)
                     /locus_tag="ACG8ZM_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0492934.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13481..13903
                     /locus_tag="ACG8ZM_001989"
     CDS             13481..13903
                     /locus_tag="ACG8ZM_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492935.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13913..14116
                     /locus_tag="ACG8ZM_001990"
     CDS             13913..14116
                     /locus_tag="ACG8ZM_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492936.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14327..14911
                     /locus_tag="ACG8ZM_001991"
     CDS             14327..14911
                     /locus_tag="ACG8ZM_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0492937.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5571 a         2451 c         3061 g         4056 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
    13381 taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta
    13441 agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt
    13501 atctttaggt attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc
    13561 tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga
    13621 taggaacgga atagccattg gtaacccaac taacgactta actgttactg aaaattattt
    13681 aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat
    13741 gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa
    13801 aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa
    13861 caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga
    13921 atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc
    13981 gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa
    14041 atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata
    14101 tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa
    14161 ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct ttatttttca
    14221 aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat
    14281 atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata
    14341 cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt
    14401 tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt
    14461 agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg
    14521 tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt
    14581 gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct
    14641 catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc
    14701 cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt
    14761 gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac
    14821 tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga
    14881 ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg
    14941 ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct actgataact
    15001 tattctcaaa tgttgtaagg attttattca tttgttaata ttaaagaacg gaactccaaa
    15061 aaagtccgta ctctaatttg cagtctctca ctcttttaga gtgagagttt ttattagatt
    15121 ttcaaattaa ttagtaaag
//
LOCUS       DAZKOR010000067        14993 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000067 DAZKOR010000000
VERSION     DAZKOR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.068"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..607
                     /locus_tag="ACG8ZM_001992"
     CDS             <1..607
                     /locus_tag="ACG8ZM_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492938.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            627..1655
                     /locus_tag="ACG8ZM_001993"
     CDS             627..1655
                     /locus_tag="ACG8ZM_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0492939.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1652..2410
                     /locus_tag="ACG8ZM_001994"
     CDS             1652..2410
                     /locus_tag="ACG8ZM_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0492940.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2496..3236)
                     /locus_tag="ACG8ZM_001995"
     CDS             complement(2496..3236)
                     /locus_tag="ACG8ZM_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0492941.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3297..4550
                     /locus_tag="ACG8ZM_001996"
     CDS             3297..4550
                     /locus_tag="ACG8ZM_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0492942.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4845..5099
                     /locus_tag="ACG8ZM_001997"
                     /pseudo
     CDS             4845..5099
                     /locus_tag="ACG8ZM_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5221..5547
                     /locus_tag="ACG8ZM_001998"
     CDS             5221..5547
                     /locus_tag="ACG8ZM_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0492943.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5615..6328
                     /locus_tag="ACG8ZM_001999"
     CDS             5615..6328
                     /locus_tag="ACG8ZM_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0492944.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6638..7490
                     /gene="licT"
                     /locus_tag="ACG8ZM_002000"
                     /pseudo
     CDS             6638..7490
                     /gene="licT"
                     /locus_tag="ACG8ZM_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7611..9476
                     /locus_tag="ACG8ZM_002001"
     CDS             7611..9476
                     /locus_tag="ACG8ZM_002001"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0492945.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9490..10933
                     /locus_tag="ACG8ZM_002002"
                     /pseudo
     CDS             9490..10933
                     /locus_tag="ACG8ZM_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11041..11742
                     /locus_tag="ACG8ZM_002003"
     CDS             11041..11742
                     /locus_tag="ACG8ZM_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0492946.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11868..12380
                     /locus_tag="ACG8ZM_002004"
                     /pseudo
     CDS             11868..12380
                     /locus_tag="ACG8ZM_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12778..12987)
                     /locus_tag="ACG8ZM_002005"
     CDS             complement(12778..12987)
                     /locus_tag="ACG8ZM_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFQ0492947.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13228..13803
                     /locus_tag="ACG8ZM_002006"
     CDS             13228..13803
                     /locus_tag="ACG8ZM_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0492948.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13803..14243
                     /locus_tag="ACG8ZM_002007"
     CDS             13803..14243
                     /locus_tag="ACG8ZM_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0492949.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14259..14960
                     /locus_tag="ACG8ZM_002008"
     CDS             14259..14960
                     /locus_tag="ACG8ZM_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0492950.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5066 a         2484 c         3161 g         4282 t
ORIGIN      
        1 agaaaatcaa ctttttgcag gagatgtatt agaaattgta ttaaacgacc aagcaggaga
       61 agctgctgct aagggtgtgc agaacaagcc aagtaccaac gatgaaattg ggaatcacaa
      121 tccaagtgat acgccagttg tttatcatga tgccccgcct tttgcacctg ctacgaagat
      181 tgtagtagaa ggtggtctct catttcttgc cccttccaaa ctcgattgtg gtcagatcaa
      241 agcaactggg ttgacgcaag aagcatttgg ccaaatggat atacaggaaa aattatggat
      301 ttatgatcag cgagaaacaa aaagtccatg gatcttaacg ataaaattat ctcagccttt
      361 cacaaccgat gcaggatttt ctatgcagga tgtcctatat ttcaagaaag ctgaggagta
      421 tcaattagtg aatgaagaag aactggaaat tgtcagagga caaaattcaa cgaatgtgca
      481 aacagattat tcagaatatt taaataatgg tcgggcgttt aaacttgtct taaccaaaga
      541 aaatcagata gctggtaatt actcagcaga gatgacatgg acattagcag atgcgccgac
      601 agaataaaga tcaaggagaa tgattgatga atcgaatatg gaataaaagt catcgaaaca
      661 actgctcttt acttctgatc cttctccttg gattcatcct ttggcagaca cctgtccatg
      721 cctccgaatt caattttgcg gtagattttg aattaccggc taatcagatt gataagaaca
      781 aaacatatat aaatgtaagg atggagcctg ggaaaacaca ggaaatcccc tacacggtct
      841 ttaaccgaac agataaatca gtaacagttg agacattgat ccactccgct acgacgaatt
      901 caaacggtgt gatcgaatat ggagaatcaa aaagtaaatt tgatgaaaca ttaagttatg
      961 atatgaaaaa cttagcaaaa acagtgggag aaataactgt ccctcctcaa agtgcggtca
     1021 ctgagacact ttcactgaaa gctccagaaa aagagctggc tggtgtggtc gcaggaggaa
     1081 tcacgtttca agaaaaaaaa gagaacgttt ctaacaaaag tacagttggt gtcgtcaatc
     1141 agttttctca agtgaaagca atcttgattc gaaatagcga aaaagagtct tccccaaatt
     1201 tgaaattaaa aaaaacttat gcggatcaaa gcgatggaag aaatgtgatt aaggcgaatc
     1261 ttcaaaatcc aaatgcttct tatatgttcg gcttgactac atcggttgaa atctacaaaa
     1321 aagaaacaaa agagccttat ctttcaataa aaaaagaaaa aatagatgta gcacccaata
     1381 caaattttga tttacttgtc ccattagaag gaaaaaaatt agctccggga cattatcaag
     1441 cggttgtgaa gggtgcttgg aaagaaaaac gctggaatct ccataccgat tttgaaataa
     1501 ctgatgatgc agcggaccaa ttgaatgaaa aggatattga tttaaaaaat cagccagaaa
     1561 catttgactg gcgatggctg atacttgttg gagtcattct tattggctta tttgctggat
     1621 tatttatttt aaaagaaaga aggaaaaaga aatgaaaaaa acaattgcat taagtaccgt
     1681 tctagctgga agtctattat tatgtacaca aagtgttttt gctgagaata agagctcagc
     1741 cactacagaa ggaacgattt ctgtaacaaa accagatgcc gcaaatcctg aagacgttgt
     1801 tccagttcct cctgttgatc caaatgatcc cgatacgccg tttacaccta acgaaccaaa
     1861 tcagggaaca tccggattac tgacgattga ccaagctcca gatcttgatt ttggaacaat
     1921 caagttaggt acagcaaaaa cagtatatgc tgcattacaa aacggaacgg acagtacagg
     1981 gacagcaaca caagtacaga atttcgtaca agtaacggat aaaagcggaa tttatgccgg
     2041 ttggaaacta tcagtcaaac gaacagaatt tattggtaca acatctgata cgaataaact
     2101 aactggttca aaggtgaaat tcaaaaacgc cgtgattcga aatagcgcaa ctaatagcag
     2161 tggaacgcca tctactattt ctggtactgg cgctgaagga attgagatcc cagcagatca
     2221 ggcagtagat ctagtaactg caggagcaaa tgaagggatt ggcacatgga tttattcatt
     2281 aggagagaat gtggaacaag ggagacaatc agtggaatta tttgttcctg tatccaatta
     2341 tgccgtagac aactacgttt caacattcac atggtcgtta acagatgctc ctacagaagg
     2401 gaaagcgtaa aatcaaatca atgacttcag ggagtggagc cgattcaagg ccaactccct
     2461 gaaatctgct atgcttacgt ctcagctctt attcatcaac tcgaagcagt tttttggaag
     2521 tcatggaaac gttcctttgt cttttcaaag atctttcgac caacgagtaa ggagtcacca
     2581 ttgaaaaatt ctaacacata atgagcagaa tcaattttct gcaattgcga taagttgata
     2641 cagtaagatt ttaatagtaa aaagtaagca tacttttcca actctgtctt caattgtttt
     2701 aaagaagtat tcactaattt atcttctgta gttgttttga ccataacttg gtttttcaaa
     2761 cctttgatac tttcaatata taaaatttcc cgaggataca ggacgagact tccacctttt
     2821 tgatcaataa agattttatc gacatttacg atttgctgtt ctttccaatt gatctgttct
     2881 agcacttgac gaatttgttc agataaattc ctgtcttttc tgatatatcc aatcggaagg
     2941 attttccgat tgatgatctc tactgcaaaa ttcgtataag cggtgaaaaa aacaatatta
     3001 ctatgtgcat tttttttacg tatttgttcg gcgacatcaa tacctgtgag tgattgcttc
     3061 aagtcgatat cgataaagaa aacatcgtga ttatcaatag tacccaactg gatcaactga
     3121 ttgatttgag agatagaagt gagctgcacg atattctcga tatgatgatt gaccaattct
     3181 ttgatgatcc attcgctgtg aaactggtta tcttctatga taaaatactt tttcatatgc
     3241 aggctcctat taaaaaaata tgtgtacatt ttgaaacaaa acaatggagg aataagatgg
     3301 tcgcaacagt gacactttat ttgaccttta ctttgatttt agatttaggt atctatttat
     3361 tcttttcgaa acaactgcta gtaaaaaaca gctttggttt tatagctttg atggtggtga
     3421 cacttttatt tcctatccct aaagtgagct ttccttacct agttggctta ttattgatgt
     3481 ttctttttta ttttactacg aaaaacctgt ttatcagctg tttgatcagt aatctttttt
     3541 ttgctatcac tggactcagc ttttttctgg tttttgatct gatggattat ttaggaaaga
     3601 gacatcttgg aacagcaagt ttttttgcac tacttatcgc atttattttc tatctattag
     3661 taaaaagtat agacaaacag acgagatttc tcaacttgct ttttacttat ccaactgaac
     3721 actattttgg aggtatttgt attttattaa gctggggcgt gatttacttg gtattgggaa
     3781 gtgttcactt ccgctcatct aattatctgc ttctatctct tttgtcgata gtcatcagtt
     3841 ttttttcttt gtttttttcc attatgttga tcagtttcca agtaaaagaa agacaacatc
     3901 aggaatcgct tcaactgtat caaataaatg aagaatatta ccatcatcta gaggaattca
     3961 aacatgatta taaaagtcta ctgttttcat taaaaattac tcttcaggaa aataaaaaag
     4021 aaacgttgga atttttagaa atcttagaag atcattctgc agctattttt gaaaaaccgc
     4081 aaatacaaca gctaacaaat attgtatccc cagctgtcag gggtgtcttt ttaaaatttc
     4141 tagaaaaagc agaatcggag aaaatcccga tccaagtagt tattcctgtc gaagtgaaaa
     4201 caatcccgat tgatctggtt gtttttattc gctgtttatc tatttttatt tctaacgcat
     4261 tcgatcatcg tgtagccgat cagagtccga ttacgatcaa tctgctggaa gaaaaagagg
     4321 gattacgttt tagtatagcg aacaaggcaa atccgacgaa tttgacgcta tcggagatgg
     4381 tcaaaagagg aaaaacctct aaaaaaaatg gcgggttagg tctatacagt gcgaaaaaaa
     4441 tgttggatgt ttacgaaaat gcagaactaa aaatcgaatt ttcaaaacag aatcactatt
     4501 tttttgtaga aatgtatctg gcatctagaa agagcaaatc agcctactga cttactgtca
     4561 aaggataagc ttgaggaaag tctacacgga gatagagaca acaacacagc atattcaaaa
     4621 gaaacagaag cgtgatgacc gttgtcaccc ttctgtttct tttgctatgt agttaaacaa
     4681 agtagttgac acttccgcta cttagtatta gtacgaaagt atacttcatg atactaagta
     4741 gataagaagt gaaaagcgga acccacgatc gtcaaaatgc taactgcttt gcttacacca
     4801 gataaaggga agagcaatat cgcttatttt aggcgtggag aagcaagaaa atccctacga
     4861 tcacacaaaa aatgctgacc cagcagttaa gagagttaga acaagatggc attgtccatc
     4921 gaaaagtcta taaccaagtc ccgcctaaag tagagtactc gctcaccgaa gaaggaaaat
     4981 cattaagaga gattctagta gcgatgtctg tttggggaga agaacgtatc gaaaaacaaa
     5041 aagcctcagg ggaagaagtc acattactgc atacacatgg tggctaccta aattactgat
     5101 ttctatgaca tttattttaa ataaaagaaa acaggagttt ttttaaaagc atcatctatt
     5161 ttttatgtaa aaaaatggta tgattaatca agtaagaaat gattggaaga aggcaagcaa
     5221 atgaatagca tggaaaaagt taaccagttt agagatgaac gtaattggcg acaatttcat
     5281 aacgaaaaag atttggctat ttctatttct ttggaagcat ctgaattgtt agaacttttt
     5341 caatggaaaa cgccggaaga agtaaaagaa acacaattag aacgaatcaa agaagaactg
     5401 gcggatgtat tgatctattc atacatgatg gcagacaacc ttcagctgga tctagatgag
     5461 atcattgaag aaaaattgat caaaaataat ttgaaatatc ctgtagaaaa aagcgcaggt
     5521 gtacgaaaaa aatacacaga gttttagaaa tgtgtcattc ggctgttgga aagagcaaaa
     5581 gaaaaacagt aaaatgagtc tggagggaga gaaaatgaaa aaaaaaggaa aagcaaaaag
     5641 atggctagtc aatatcctct tgttcctctt gttattagta ggactagctt tgattttcaa
     5701 tgaacagatc aaggattatc ttgtcagaga gacaggagat aaatatgcta tagcgaatgt
     5761 aacaaaagaa gatctgaaaa agaataacga taaagatgtc agttttgact ttgatgcggt
     5821 agaaccaatg acgacagaag gggtcatgag gtcgcagatg agaggaacag atctaccagt
     5881 gattgctagt atcgcagtcc cttcagtttc aatcaattta ccgattttca aaggcttgga
     5941 taatacgtca ctactttacg gagcaggaac attatctcca gatcaagaaa tgggaaaagg
     6001 aaattatgcc ttagcaagcc atcgtgcaac gaaccctgaa ttgttgttta caccgctgga
     6061 aaatttagag atgggtgcca agatctattt gacagattta gaaaatgtgt atacatacaa
     6121 aacatttttt aaagaaaaag ttgctcctac agatacccaa ttactgaatg aagtcgagga
     6181 aaaagaaatt gtcacactaa ttacttgtgg cgacatggac gccgtcacgc gactagttgt
     6241 acaaggagaa ctggaaagtg ttacaccgat aaaagatgcg acagatgata tgagatctgc
     6301 tttcaatcta gagacaaaaa cattttagga tactgggaca taagtcgtta cagattaaaa
     6361 acagccaaag gatatgaatc gataaggaaa aaccaatttg ctgaaaaaaa gaggaacgca
     6421 aagaggtgca tctctgattt ggttgttttc tagatagact tgtgttccag tttttttatt
     6481 ttttatatga aaaggcttgc ataaataaaa attcgatggt atgatgattt tgaataagaa
     6541 atggattgtt actgtacggc aggcaaaacc agaattcagc taagaaacag taggcgaatt
     6601 ctaggttttt ttatttttct tgagagggcg aagcaaaatg aaaattgata aaattttgaa
     6661 taacaatgtc gtcatttcga aaaatggatt cggggaagaa gtcgtttgta tgggaaaagg
     6721 actagctttt caaaaaaaag attggggacg agatttcgcc agaagcggtc caaaaagaat
     6781 ttattttaag agattctttt gctaaaaacc aattccagca gctgatggct gatattccgg
     6841 cagaggagat cgaatgggtc aaacaagtag tcgaactggc tgaagataaa ctaaaagttg
     6901 aattttcgcc taatatttat ctgacattga ccgatcatct tcattatgcg atcacacgag
     6961 caaaagaaaa tatccaactg cctaatccat tattatttga aacaaaaaaa ttttatccga
     7021 aagaatatca agcagcgaaa gaggctcttt tgttcgtgaa acaaaagacg ggtgtggaac
     7081 tttctgagga tgaagccggt tttattgcct ttcattttgt gaatagccaa caaggaaacg
     7141 aagacatgca agtgacgatg accgctacca caatggtaag agatatactg agtatcatca
     7201 gtaaattctt cggcatcgtg ttcgatgaag aatctttgaa ttatcagcgt atcattactc
     7261 atctgcagtt ttttgcccag cgttatctca aaggggaagg atctgacgag caagatgaat
     7321 ttctctacgc attagttcaa ggaaagtatc caaaagcgtt tcattgtgtc gaacggatca
     7381 atgagtatct tcgacaaacg caaaaacagg aaatggggat cgcagagcag atttatttaa
     7441 cgattcatat tcaaagagta gtaagtgaga aaaaaagcat ccaatcataa ggattgttac
     7501 tgtatggcag gcaaaacctg gattgaaaaa ataggttcac gaatgtttgt gactgttttt
     7561 ttcaatctgg gtttttattt tttaaataaa aaagaaagaa ggaaaaaaca atggataatc
     7621 aagcagttgg tcgtcgtgta tgggaagcgg taggaggaca aaagaatgtc aaaagtttag
     7681 tacattgtgc tacgcggttg cgattcagat taaaagatga atctttagca gatacgcaaa
     7741 agttaaaaga agacccggac gtgatccaag tcgtacaaag tggcggtcag tatcaagtag
     7801 tgattggcag taatgtagca gatgtttatc aatcgatcgt ggatgaggaa ggtcttgcag
     7861 tttcggagga tggaaaagaa gagtccaaaa atattttgaa tcgtctgatc gatattatct
     7921 ctagtatatt tacacctttt ctaggggcga tggcagccgc cggggtattg aaaggattct
     7981 tatcattggc aacggtgatg ggctggctga cagctgatag cggtacttat caaatattgt
     8041 ttgctgcagc agatggtgtc tttaccttct tacctgtcat gcttgctttt acagcagcta
     8101 aaaagttcaa agccaaccag tttttagcag tagcaattgc tatggcactt gtgtatccag
     8161 ctatcacagc agttgctggt gcgggagaag ctatcagttt ctttgggatc ccagtgattc
     8221 tttcaccatc tggttatact tcttctgtca taccaatcat tctagccgta tgggttcaaa
     8281 gtaaattaga gccattcgta aaaaaagtga ttccgcaatt tctgcaaatg attttagttc
     8341 cgcttgtcgt tttagtagtg atggttccat taacatttct tgctttagga ccaatcggga
     8401 cggttgccgg taatgcttta ggcggtctct tcaactcgat ttatggattt agtccaatcg
     8461 tagcaggtct aatcatggga agtttatggc aagtattcgt gatgttcggc atgcattggg
     8521 gattcgtacc gatcatgttt ttgaatatcg aacaatatgg gtttgatgta ttgatgccaa
     8581 tgcttcttcc agccatcttg gcacaaggtg gggctgcttt agctgtagca ttgcgaacaa
     8641 aagatacgaa acttcgagca ttagggattt cttctacagt gacttcgtta tttggaatca
     8701 ctgaaccaac tgtgtatggt gtgacattac ctttgaagaa accattcatt gccgcttgta
     8761 tttctggagg tattggtgga gctattatcg gattttccgg agtaaaagct ttctccagca
     8821 gtttggtcag cttattgacg attccgactt tcattaatac ggtggatggt gtagagtcaa
     8881 atgtgacagt agcagtaata gctacaggaa ttgcttttgt tcttgcattc gtaggtacat
     8941 tgattttagg atttgacgaa caaacacaag acaatcaatt agaaaacaaa catgcgaatg
     9001 caggagaacc gattacttca gcacggcata ccttaaaaag tcctttaacg ggtaaagtac
     9061 tgccattgag tgaagtacca gaccaagttt tttcttctgg cgtgatggga aaaggtatcg
     9121 ctattgatcc agaagtagga gaactagttg cacctgcaga cggagagatc acgactattt
     9181 ttccaactgg acatgctgta gggatcacaa cgactgacgg agcggagatt ttgattcata
     9241 tcgggatgga tacagtagaa ttaaatggga atggctttga gatcttagtc aaacaagggg
     9301 atctcgtcaa agcaggagat ttattgattc gttttgacat cgaagcaatt agagcagctg
     9361 gctatagcgt aatcactcca gttgtcatta cgaatactga tgcatttgcc gatatacttg
     9421 aattagacca aaaagagatt attgcaaacg aagatgttct ggcaattgta aaataaggag
     9481 gaaatcagga tgcaatcacg tttttcagaa aaatttttat ggggtggagc aactgctgcc
     9541 aaatcaatta gaaggggctt atgatcgaga cggaaaagga ctatccgttg cagatgctat
     9601 gcctggaggt aagcagcgct ttgcgattat cggcagtgaa gaatttgact ggacgattga
     9661 tcaagaaaaa tatatttacc cgaatcatcg aggaatcgat cattacgatc gtttcaaaga
     9721 agatcttgcc ttgtttgcgg aaatgggatt caaatgttat cgtttttcga ttgcctggac
     9781 acgaattttt ccaaatggag acgaaggtac tcctaacgaa gcgggattag aattttatga
     9841 tcagctgatt gatgagtgct taaaatatga tattgaacct gtgatcacga tttctcatta
     9901 cgaaatgcct cttcatttgg caaaagaata cggtggctgg aaaaatcgaa aactgatcga
     9961 cttctatgaa cgatttgctc agacagtatt agagagatac agcagtaaag tcaaatattg
    10021 gatgactttc aatgaaatca attcggcctt tcactttcca gctttaagtc aaggattagt
    10081 aaaaagcaat ggagcaggag agtaccaaaa tattttccaa gcatggcata atcaattcgt
    10141 tgcaagtagc aaagccgtaa aaatcggcca cgaattgcgt tcggatatcc aaatcggctg
    10201 catgattatc tatgcaacaa cttacagcat cgatgccaat ccagtcaatc aggcagcaac
    10261 aatgatccaa aaccaagaat tcaatttttt ctgcacggat gtccaagtac gtggagaata
    10321 ccctgcatac acagcgcgta cccataaaaa atatggagtc gatccggaaa aattagagca
    10381 aacggaaaaa gacttccagt tgctaaaaga atatccagtg gactacatcg gatttagtta
    10441 ttatatgtct acggcaatca atgagactga tccacaagca gcaacttcag aagggaactt
    10501 gctgggagga gtaaaaaatc cgtttcttga ggcaagtgaa tggggctggc agatcgatcc
    10561 agaagggctg agaatcgcat tgaatgaatt atacaatcgt taccagaaac cattattcat
    10621 tgttgaaaac ggtcttggag ccattgatca tatggaagag gatggcacga ttattgatga
    10681 ttatcggatc gattatttac gtcgtcatat cgaagcgatg gctaatgcag tagcagacgg
    10741 tgtagatttg atgggctata cgccttgggg atgtatcgat cttgtcagtg catcaacagg
    10801 agaaatgagt aaacgttacg gctttatcta tgtggatcta gacgatgaag gaaatgggac
    10861 actgaatcga tcaaagaaaa aatcttttga ttggtataaa gaagtaattg cctcaaatgg
    10921 cgtaaacttg taattaaagc agctgaatga aaaagaacac acttctcaca tgtgatttgt
    10981 gttctttttt tattttttat gtgctattct tttagtaata gtaaagcaaa gggtgacgtt
    11041 atgaaaagga cacgtgtact atacttggaa gtcgcagata aaattaaaga agatattttc
    11101 tcaggaaaat atccagtcgg ttcgatgttg ccgacagaaa gtgaattaga agaactgttt
    11161 caagtcagca aaatcactgt ccgaaaggca atcgaactgc tggcatcaga tgaatatgtc
    11221 gcaaagaaaa gtggtcgtgg gacgacggtt ctaagcaatc gtccttacaa tcgattatcc
    11281 aaagcggcaa gctttacgca aatcttggag aactcccgat atgaagtccg gaaagaaacg
    11341 ctggatttgc aattggtcca tataaagaag gaagatcctt tgttttcctt tttcggtgag
    11401 caagctgttt gttttaagcg cttatattat ttgaacaatc agccttatat ttattttgtt
    11461 cattatcttc cacaagaaat gatgcaattc agcagagaag aatttggcaa agaatctttg
    11521 tatcgtttgc tgacgaaaaa aggatatact attgactcat tttctgatga tttctcggca
    11581 gtatttttaa cagaagaaga acaaggaatt ctgaaaacag acgcacagat cggcatcaaa
    11641 cggatacgga aatcgtctac gcaaattggg gaaatgattg aatattcgga ggcggtctac
    11701 catacggagc tgcatcctta ccatattgat tatgaaacat aaataaaaaa agaggttgtg
    11761 aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt
    11821 tcgtttggtt aggcttatta ccgcagatac acggctgttt ctggattacc atttattctg
    11881 gataaggggg tgtggcacga ttttcagaag gaatctatga accaatcact gtattgattt
    11941 tagcttcgat ggtaacccca ttcggcttga cgatcgctta tttcttaggg aaaatcattc
    12001 gtaaaaatat actcaatcgt caagaaatcg atacattgaa gaccgctttt cctatgggga
    12061 tctgtcagat cacagaggga tgtttcccta tcgtattgaa cgacttggca cgcaatgtca
    12121 tagcaacggg agtcggtggg acagtaggtg gcggattaag catgttttgg ggcgcagata
    12181 gccgtattcc ggcatcggga atgtttgcag tcgctacgat gaccagacca tgggcattca
    12241 ttggtgcatt gttagctggt tcttttgtca caggaataac cattttatta ttgaaaaaac
    12301 cagtagatcc gaatgcagaa atcatccaag aagaaaaaga ggaagaagac atatcttggg
    12361 atgatttgac aattagttag gagaggcaga atggattaca agaaaaaagt acaatagatg
    12421 taccttgaga caggaatggt atgaaagaat cagagttaga aagaatcgaa tatacagatt
    12481 ttggtttagg agcgatcgaa actgtccaga gcatcggcac cctccttgaa atgacgatga
    12541 aggaaaaatg agaacctttc gttgtcgaaa gggtctggta taccttatgt cgaaggaaag
    12601 gaaacaaaaa aactttcctg tactattcca aaaaggaaag aaagttatta tacagtaaaa
    12661 aaagaaatta ttcttcatgc tagagatcag tacacgttcg agcccaatat caaacactag
    12721 ttccaggcag gcgcaacagg tgcggttatc actgaatttt cttccagcag tgacgattca
    12781 agtgatattt ttacaaatcc agctatttat cgttaacaaa aagattttta ggagaagctg
    12841 atgaacaaaa atgatcaaat tttgatcgtt tagttcgtcg gcttttttat ttagagaaac
    12901 atcatttatc agtgaaacat aacacgagaa caaaatgatt ttttcgttaa taaaagaaac
    12961 aagtatttgt tgtttaaaat aatacacgtt attggcatat aggacgaaat cccagtatct
    13021 tctataagta agtcatttca tatcgatgaa aacacgttat ttatttttgt tttatctata
    13081 aaatggctga aaaatgattt ttttctaaat actatatata gtggcgtcta tcctatatac
    13141 cactaaaaaa ctacaaaatc tagtctttta ttttaaaaaa acacgtgtag aatgaagata
    13201 gacttttgag ttagggagtg tttttttatg aaacttactg ttttttgcgg ttcccgtttt
    13261 ggtaataaag aaagttacaa atttattgcg caaacgttag gaaaatacat ggcacaagaa
    13321 gaaatcgaac tggtttacgg tggttcatac tccgggatta tgggagtgat tagtaaaaca
    13381 gtactggaaa ataacggaaa agtcacaggt atctatccga atgggctttt tgaaactgaa
    13441 ttgcctggaa aagatgttac tacatttatt cctacagaaa cgatcgatga aagaaaggta
    13501 ttattatttg aaaagggaga tgctgtcctc gttttccctg gaggtctagg tacattggag
    13561 gaattctcac aacttctttc ttggatagca atcggtctga cgcccgacaa gccaatcgga
    13621 atattgaata ttggcggtta ttacagcggc ttgcaacaat tattagaaac ttttgcaaaa
    13681 gaggatttta tggataaaaa atggttggat caagtaatct tttccaacaa tccattggaa
    13741 ctggtcaatg aattacgagc agtatctatt aaaaatcagt tattattgaa ggaggcaaaa
    13801 taatgagtca aatatattta gcaggaccat ttttttcaga ggagcaaatc gatcgagtaa
    13861 gtcgtatcga aaaagcgttg gaggaaaaca aaacagtcac aagtttctac tctccacgtc
    13921 atcaccaaga aagcaattat gaactattca gcgctggatg ggcgcaggaa gtatacgaaa
    13981 aagatatgga agaactgaca aatgcagaat ttgttgttgc tatccttgac tttgagcatc
    14041 aaacaatcga tccaggaacg gcttacgaat taggtgtggc aactatgttg aaaaaaccga
    14101 tgatcattgt acaagaagag acggtaccta cgaacttgat gattacacaa agtctccata
    14161 cgtatttaaa atcagaccaa gctgtacgtg aatatgattt tgaaacattg cctgttgaaa
    14221 cttacgtagg cgagtactta tagaagagga agggttgtat gtcagtgatt ttgatttcag
    14281 gaacgattgg tgcaggtaag agcagtttga cggatatgct tgcaaaagag attgattcta
    14341 aaccatttta tgaaaatgtc gaagataacg aagtattgcc gctcttttat tcaaatcctg
    14401 agcagtacgc ttttttattg caaatcttct ttttgaacaa gcgattttta gcaatgaaaa
    14461 atgctttagt gaacgatgac aatgtgctag accgatccat ctatgaagat agcctgttgt
    14521 tccatctaaa cgctgattta ggtcgggtaa cggatatcga agtacagcag tacgatagct
    14581 tattagatac gatgctaaac gagttagatg atgttgctcc aaaaaaacgg cctgatttga
    14641 tggtccatat caaagtttct ttggacacga tgctagaacg tatcaaaaaa cgtgggcgtg
    14701 actatgaaca gctagaaagc gatgagacgt tatataccta ttatgaaacg ttgaacaccc
    14761 gctataatca atggtatgaa gattttgatg tatgtcctaa aatacaagtg gatggggata
    14821 aattcgattt tgtagaaaat ccagaagatg caaaaaaggt gatccgtcag atccaagaaa
    14881 aattagctga attggaagga aagcctgctg gaaaatattt tacggataac cagcatgcaa
    14941 aaggtcaaga attaggctaa aaataaaaaa ccaaacgacg ggaaagaaaa att
//
LOCUS       DAZKOR010000068        14609 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000068 DAZKOR010000000
VERSION     DAZKOR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.069"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            279..1127
                     /locus_tag="ACG8ZM_002009"
     CDS             279..1127
                     /locus_tag="ACG8ZM_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0492951.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="ACG8ZM_002010"
     CDS             complement(1298..1639)
                     /locus_tag="ACG8ZM_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFQ0492952.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="ACG8ZM_002011"
     CDS             1725..2432
                     /locus_tag="ACG8ZM_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0492953.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="ACG8ZM_002012"
     CDS             2419..2742
                     /locus_tag="ACG8ZM_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0492954.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3868)
                     /locus_tag="ACG8ZM_002013"
     CDS             complement(2831..3868)
                     /locus_tag="ACG8ZM_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0492955.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4315..5298)
                     /locus_tag="ACG8ZM_002014"
     CDS             complement(4315..5298)
                     /locus_tag="ACG8ZM_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0492956.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5714..6484)
                     /locus_tag="ACG8ZM_002015"
     CDS             complement(5714..6484)
                     /locus_tag="ACG8ZM_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0492957.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6477..7412)
                     /locus_tag="ACG8ZM_002016"
     CDS             complement(6477..7412)
                     /locus_tag="ACG8ZM_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0492958.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8006..9655)
                     /locus_tag="ACG8ZM_002017"
     CDS             complement(8006..9655)
                     /locus_tag="ACG8ZM_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0492959.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9850..10746)
                     /locus_tag="ACG8ZM_002018"
     CDS             complement(9850..10746)
                     /locus_tag="ACG8ZM_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0492960.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10739..11419)
                     /locus_tag="ACG8ZM_002019"
     CDS             complement(10739..11419)
                     /locus_tag="ACG8ZM_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0492961.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11420..12202)
                     /locus_tag="ACG8ZM_002020"
     CDS             complement(11420..12202)
                     /locus_tag="ACG8ZM_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0492962.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12195..13112)
                     /locus_tag="ACG8ZM_002021"
     CDS             complement(12195..13112)
                     /locus_tag="ACG8ZM_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0492963.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13115..13399)
                     /locus_tag="ACG8ZM_002022"
     CDS             complement(13115..13399)
                     /locus_tag="ACG8ZM_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492964.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(14100..>14609)
                     /locus_tag="ACG8ZM_002023"
     CDS             complement(14100..>14609)
                     /locus_tag="ACG8ZM_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492965.1"
                     /translation="ECKRLLGENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKA
                     FISNGESLKNVTARIRKENEVDIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVY
                     LYDILIPKLRSLYMSSESIFVSEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQK
                     NNNTKIQVS"
BASE COUNT         4341 a         3116 c         2516 g         4636 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat
     3601 cgccgacaga aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa
     3661 aacctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     3721 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     3781 gttctttttc atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga
     3841 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     3901 catccataca gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc
     3961 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     4021 tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc
     4081 acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     4141 agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg
     4201 gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac
     4261 ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     4321 ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc
     4381 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     4441 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     4501 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     4561 cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc
     4621 aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat
     4681 cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc
     4741 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     4801 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     4861 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc
     4921 cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     4981 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5041 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5101 tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5161 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     5221 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     5281 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     5341 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     5401 aaaagatgtt tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt
     5461 ttccggaata aatggtgttc aaaagcttgc atctgcggta ataagtccaa ccaaacgaaa
     5521 aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
     5581 caacctctcc actaaacggt gttcaatcag ctgaccctcg gtattcgctc aaaaaacaac
     5641 aaaatatgag gaactatttt cccgtttgtt tctctaatac tcaggtcaaa acgctgattt
     5701 cacaacctct tttctaactt ctcaatcctc ttcctttatt gatcaaatac caagtaacgg
     5761 cgtacaaaac aacgatgaat aacacaagta cgatcatcga tgttgctact ggcacatcga
     5821 tcgttccaag gaatccgtaa cggaaaccgg agatcatata aacgatcgga ttgacttttg
     5881 aaacagcttg ccaaaagggc ggtaacatag agatggcata gaaaacgcca cctagataag
     5941 ttaacggctg caagacaaat gtaggcacga tcgatacatc atcataagac tgagcaaaaa
     6001 tcccgttgag cagtcctgct aatgaaaaca agattgccgt catcaataat gtaatgatga
     6061 ccatgaacca tgaatacaca tgaagtggta caaagaacaa ggaaatgatc gttacgagag
     6121 agccgacgag aatactgcga cccacaccac caatcacaaa gccccaaata atcacatgcg
     6181 taggaacagg tgctaccaag atttcttcga tatttttctg aaatttttga gagaagaagg
     6241 acgaagaaac attggcatac gaactagtga tagcagacat cataatcaaa ccaggaacga
     6301 tgaattccat atatgaaaaa ccacccatgt caccgatccg cccaccgatc atttttccga
     6361 aaataacaaa atacaaggat gtcgtgatca ctggcggaac cagtgtttgg acccatatac
     6421 gcaaatagcg gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa taaagattaa
     6481 acatttttct cctccgtatg attattttct tctgtgattt tcaagaacag ttcttctaaa
     6541 cgattcgact tattcctcat agaaagaact ttgattcctt gtcttgtaag ctgatcgaag
     6601 attccgttca ccccttgatt tcgttcaact tcgacagcga ttgtctgctc atcttcaaat
     6661 gtacaagcat atccttcaat cacaggtttg gtatcatatg gcgctaaatc aaagataaat
     6721 gtttcaaatt gcagttttga cagaagctct ttcatacttg tattctcgat cagttcaccc
     6781 gattggatga ttccgatgtt gcgacaaagc atttcagctt cttctagata atgcgtcgtc
     6841 aaaatgatcg ttgtaccatt ttcgttcaat tctcgtagaa actcccacat ctcgcgtctc
     6901 aactcgatgt caactcctgc tgttggttca tccaagatca gcaaacgtgg ttcatgcatc
     6961 aatgccctag caatcatcag tcttcgcttc attcccccgg aaagcatacg tgcacggaca
     7021 tttctctttt cccaaagatt ggactgcttc aaatattttt cgctgcgttt catcgcttct
     7081 ttgcgagaga caccgtagta gcctgcttga ttcacgacga tttgctggac cgtttcaaaa
     7141 gggttaaaat tgaactcttg tggaaccaat ccaatctgtt gctttgctcg aactagatcc
     7201 ttgtccagat cataaccgaa gacattcact tttccagatg ttttattgac taaagaagta
     7261 ataatcccga tcgttgtaga cttcccagct ccatttggcc ctaatagcgc atagaaatct
     7321 ccttctttca ccgaaagatc gattccccgc aatgcttcga ccccacttcc atagatcttt
     7381 ttcaaattat ttatttcaag cgcatttgcc atttatttcc tatctcctta tagacatgat
     7441 tcttaattgt atcatggaat acagcagatg aaaaaacaac ttggcttcca cacaaaaaca
     7501 cacctgctaa caaagaggag atgtgttttc tagttcatga tcatcttcga tagaataact
     7561 atctaaacaa ctctggaact tttaaaatca taaaagtacc gacagcaact actacgatga
     7621 catatagaag atccttataa ctgaggttct tgtctttttt attcatgtta agctttcctc
     7681 cgtcctttta tcgaaagctt ttttctttta ttttaaccga aataaataaa atggaaatag
     7741 ttattttcaa tttctgtttt cgctgtttgt tttacggaga actttgccat ggaacgtgcg
     7801 tctcgtcgct gatgctaagt tttttcgata aaagctgcta gaaggaagac agcgtggttt
     7861 attttgtatc gtcttttgct aaatcttcca agatcatctg gacagcagac tcgttcccat
     7921 tgaattctgc cacttttttg gcatcaatgt acttcttttg ataatcgtct ttttggaccc
     7981 acgcatcatg cttcgtttga taaagttaat caaacggata ccactgacgc tgttcatcga
     8041 acaacaaact ttctttttgg gcctgttcaa ctatggattt aggataattc aaaactttca
     8101 gcaaggcaat cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga
     8161 ttccttttcc ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa
     8221 tctctgtcaa ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc
     8281 accgaacgac tgaagcggaa gaagcaatcc gttcgatcgt atttgttcct tttaaaatct
     8341 catctacgaa gcagtagcag cgttctttgg aagccacttg gtccagcaaa cgtttgatcg
     8401 atttgatttc tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg
     8461 aagtcaacac atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct
     8521 gtgctagaat acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg
     8581 aacccgtgac taatgtattc ttctgccagt tgactggatt aactaccgct tcttttaaca
     8641 atggatgata gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga
     8701 tcggcatata ggtgcgataa ttcaaaacgg ctgctgcaac ttccaattct cccagcaatt
     8761 cccacagctt gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg
     8821 agataaatgg cagcatgaac atgatgttga catattcaaa aatcaattct gcttcactcc
     8881 cgctcttcat tcgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct
     8941 gtttcagctc tttttgaagt ggtgtcgtca tttttgctaa ctgattggcg gctgaaatcg
     9001 ttgaaaccaa ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat
     9061 aatagaccgt attgaatact aagccaccga ttgctagata gattcctagt acttgcccta
     9121 aaagaagcaa acctacaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc
     9181 gtccaatctt tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt
     9241 ttcctaaacg agcgaattga tattggattc tttctctcag ttgaggattt tcctgataaa
     9301 aagcaattag tttttctaac tgataatctt ctccaaaatc aaagctacgt aaacgctgat
     9361 acaacgcttc tgaacctaca ctagaatagg tactgttgat cccttcaaat acttcgaaca
     9421 tatccaagtc ataccaagta agatcgtcga tttcactatc ccatgaacgg aattttttct
     9481 ccgtgagcca cgcatccttc aaactttctt ctttatcaaa tctcggttga taaggaattt
     9541 ttccccaatt actacgaact gtttccttta ctttcaaacg tgaaaaaatg tctaaaacaa
     9601 ctaataaaat aacaactgca ataataatta aaacgataaa ttctctttgg cccatatccc
     9661 tctccgttcc ttgatttgtt agttttagta tagcactgtt gcgctatttg aaaagggggt
     9721 acaaaaataa ctgtcttcgt tacctaattc gtatcaatca caccaaaata aaaacacctt
     9781 ccaaaaaaga agatgtcttt aaaacgagat acaatttttt gtcattcact tcaggtaaat
     9841 tagagccaat taaaccctcc gcaacttgac ggagatggag aagatgcccc cgtctaatct
     9901 agcggtcatc tctcctccca ttccttccac caattttttt gacaggtaaa gcccgattcc
     9961 gctagattcc gtatttgtgc ggctggtatt ttcggtataa aaacgctgga agactttttc
    10021 aacagaggtt tgcgctcctg gtttgatagg attggccact gttaatcgaa tgaatgaatc
    10081 ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa cgcaataaat tctgtatgat
    10141 attttgcagc actcgcagca acatgagcgg atctgctaat acagttgtat ttatcgagtg
    10201 ttcagcgaac gctacctcga tatttttctc agaaaattgt tcataaaaag agaaaaatac
    10261 ttcttccacc tctttagaaa aagaaagcgt atggagatcc atttcttttt gcttcgtctc
    10321 tattcgagca agatcataaa actgatccgt catactgagt aatcttgcaa cagattgttt
    10381 gatgatcgtt aaatacttct cccgtttctc cgtatcctct acctgttcca gtaattgtac
    10441 ataaccgtta atagcagtca aaggagttct gagatcatga gaaaggccga cgatcgatag
    10501 tctgatgttt tcttccattt cttgaaatgc ttgtcctttt tgttcatttt catccaccca
    10561 tgtattcagt gcatctacta ggtccattag attcttttct cgaaattcga ctgaaagacg
    10621 accaccatat tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc tcttgatttc
    10681 cctttgcagg aagaaaataa aacttcccat aaagataaca acgagaagca acagccactt
    10741 aatcaaatct aaaccccact ccccacaccg tatgaatata cacagcatgt gcctttgcta
    10801 gtttccctcg aagattggaa atatgcatat tcactgtttt ctcactatca aaatatggtt
    10861 cttgccaaac tgtttcataa agatttgctc gcgaaaatac ttttttcgga tgttctaaaa
    10921 aaagcttcag cagatcgaat tctcttcctg tcaaatggac agccacacca ttcacattta
    10981 catccctcga ctctttgtcc agtaagattt ccttgtaata gatgcgttgc gacagatctt
    11041 ttacttggac agaagtctga tgacgaagtt ggatgccgat tcgtaacaac agttcttcga
    11101 tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa actttatctc
    11161 tttgatcgct ttttgctgaa agaacgatga tcggaacatt cgatgtctgt cggatttctg
    11221 ctaacaatgc ttcacctgaa agccctggaa gcatgagatc tagaaggatc aaatcgacag
    11281 gatatgtgct taacaataat tttccttccg tacctgaata agcatcaaga agtttgtatc
    11341 tctgttcaag aacctcctcc agtaaagaat gaacagcttc atcgtcttcg ataatcaaaa
    11401 tagtttccag caggaacacc tacttaatgt cagatttttg gaaaataaac attcctagag
    11461 ctgatgatac cacgattgtt actagactga ccagaatcat tttcggtgct tgcgaataag
    11521 ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac tccgttagaa
    11581 attccaagcc tttgattttt gcgaaaacac cgggaacgaa ggccgttccc atgatataaa
    11641 gaaaagaaag aagcaaactg acactggtac ttctagtgat gaatgtcaaa gccactggaa
    11701 cagataatgt cgctaatatc agcagaagca atacaggaaa tacttttaga acataatttc
    11761 catagttttt gattccctca acttcaccaa atccgaaagc aagtgtgtaa gcaatcatgg
    11821 ctagccccgt aatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg
    11881 acacataata agtcattcga ttccgtccta aagataacgt attgcgaatc gtaccgtttt
    11941 gaaattcttc cccaatataa aagcacgcaa atgctgcgaa agccagaaga atccatgcag
    12001 ccattttttc caccattaaa atccctgttg caccgttgat gactgtatcc gttgcttggt
    12061 ttaattcatt gccgtatttt gtatgcatat accattcgtt ccaagaataa aaaagcggaa
    12121 gtacgaccat cagtcctagt gagacccaca ttttacggct cttccatact tttatttttt
    12181 cagcagttaa tagattaaac atgtggattg cctccagtta attgtaagaa ataatcttcc
    12241 agtttttgcc caactaggtg gataccatcc acaggaactt ttgcctctac caatgcttga
    12301 ttgatggctg ctacttggtc aattccttca aaaagctgga taacacggct agattggaca
    12361 aaataatccc tataaccaag agagtcgaga accgttactg cttgttccgt cgccgaagtg
    12421 tcaattttta taaattgacg agattcttgt gccaattctt ctttcgataa ttctttgatc
    12481 aagtgtcctt catgcaaaat accgtagtga gtggcgatct gtgacaattc atctagcaag
    12541 tggctagaaa gcaagacggt gatgccccgt tcttttacca aacgttggat gatttctcgc
    12601 atttctgcca tccctgctgg atccatccca ttcgtcggtt catccaaaat caaaaattct
    12661 ggattcgtga tcagtgcatt tgcaatagct aaacgctggc gcatacctaa agagaaattc
    12721 tttgctttct ttttccctgt attttccaat cgcatcattt tcagcaggtc ttcgatttct
    12781 ctatccgaca caccgccgtt cgctgcttgg atacgtaggt tatctcttgc agttaattct
    12841 ggataaagag caggggtttc aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc
    12901 gtcgttccaa ataattcgat cgaaccttca tcaatcgaga tcaaacccat aatagttctc
    12961 ataaaggtag acttacccgc tccattctcc ccaatcaaac catagatcat tccacgtttg
    13021 atttcgatcg atactttatc taatgctttg gcagcaccgt attttttggt tataccagtt
    13081 gctttcaaga ccgtttcatt tccaaaatcc atacttatcc ttctttctta ttgataagtg
    13141 aagtatagtt gacgggtata aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt
    13201 ccaattgaat ttcaagaaaa atcagactgt ctcgcagacg tggttgagtt gtctgccaat
    13261 caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta
    13321 tcgttgacat cagacgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat
    13381 aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt
    13441 ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct
    13501 tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca
    13561 aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca
    13621 caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt
    13681 tatagatcga atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa
    13741 aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct
    13801 cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt
    13861 aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggttaa acaaaactct
    13921 attttattgc attttacagg aatgtatttc atgcgaaatt aataagtctt tgtttataga
    13981 gttttactag ggtataaaaa gttgtatgtt atcattacct caaaacatta cctcagcttg
    14041 gcgagttggt tgattgaaga ataaatcatt tatctagata tctcaataaa aaatgatttc
    14101 taagaaactt gtattttcgt gttattattt ttttgcccta tcaaaatttc taattcttct
    14161 agcgtaatat gattatgaca cataatcgat tcaattactt caatacctat ttttttcgag
    14221 aaagatccct tttgttcact cacaaaaata ctttcagaag acatatataa ggaacgaagt
    14281 tttggaatta gtatatcgta caaatagacc accataaaat cgtccttgca accattctca
    14341 aaaagaatta aatttgctga ttgtgttaca tatatcactg aattacattc atggtcaatt
    14401 ccaaaaacaa tgtctacttc attttctttt ctaattcgcg cagttacatt tttcaaactt
    14461 tcaccattac ttataaatgc tttagttact gtaagctctg atgtttgttc agatattcct
    14521 tttataatat tttgttcatc agccacattc cccataaatc cgccgattcc ttttaagtaa
    14581 taattctcac ctaagagtcg cttgcattc
//
LOCUS       DAZKOR010000069        14591 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000069 DAZKOR010000000
VERSION     DAZKOR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.070"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(197..523)
                     /locus_tag="ACG8ZM_002024"
     CDS             complement(197..523)
                     /locus_tag="ACG8ZM_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492966.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(535..735)
                     /locus_tag="ACG8ZM_002025"
     CDS             complement(535..735)
                     /locus_tag="ACG8ZM_002025"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0492967.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            858..1460
                     /locus_tag="ACG8ZM_002026"
     CDS             858..1460
                     /locus_tag="ACG8ZM_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0492968.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1515..2681
                     /locus_tag="ACG8ZM_002027"
     CDS             1515..2681
                     /locus_tag="ACG8ZM_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0492969.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            3098..6325
                     /gene="fss3"
                     /locus_tag="ACG8ZM_002028"
     CDS             3098..6325
                     /gene="fss3"
                     /locus_tag="ACG8ZM_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0492970.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            6437..6751
                     /locus_tag="ACG8ZM_002029"
     CDS             6437..6751
                     /locus_tag="ACG8ZM_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0492971.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            6764..7138
                     /locus_tag="ACG8ZM_002030"
     CDS             6764..7138
                     /locus_tag="ACG8ZM_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0492972.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            7148..7492
                     /locus_tag="ACG8ZM_002031"
     CDS             7148..7492
                     /locus_tag="ACG8ZM_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0492973.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            7561..8577
                     /locus_tag="ACG8ZM_002032"
     CDS             7561..8577
                     /locus_tag="ACG8ZM_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0492974.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            9171..11045
                     /gene="ltrA"
                     /locus_tag="ACG8ZM_002033"
     CDS             9171..11045
                     /gene="ltrA"
                     /locus_tag="ACG8ZM_002033"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0492975.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            11147..11527
                     /locus_tag="ACG8ZM_002034"
                     /pseudo
     CDS             11147..11527
                     /locus_tag="ACG8ZM_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            11572..12714
                     /gene="dcm"
                     /locus_tag="ACG8ZM_002035"
     CDS             11572..12714
                     /gene="dcm"
                     /locus_tag="ACG8ZM_002035"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFQ0492976.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            12704..12993
                     /locus_tag="ACG8ZM_002036"
                     /pseudo
     CDS             12704..12993
                     /locus_tag="ACG8ZM_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13249..14433
                     /gene="mobT"
                     /locus_tag="ACG8ZM_002037"
     CDS             13249..14433
                     /gene="mobT"
                     /locus_tag="ACG8ZM_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0492977.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            14430..14543
                     /locus_tag="ACG8ZM_002038"
     CDS             14430..14543
                     /locus_tag="ACG8ZM_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0492978.1"
                     /translation="MKRGILMEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         4938 a         2486 c         3009 g         4158 t
ORIGIN      
        1 tagccttgct tttcaactaa gctttcaact tctttttcat tcattgtcac acctgcttct
       61 gattttattt gctataatag attcaaataa agttttctca gaactttatt attgagtgtt
      121 cccccgatct gcaaactgac gggaacactc tttttaattt tttctacttc atcaaattta
      181 aaaaacgagg tgttttttaa atttgatgac tcaatatcca ttttttgcat tcttcttttg
      241 aaaacaactc cattttttct aataaaataa ctggcaatcc cattctttta aatttcaaaa
      301 aagttgtata tgatacattc aaatatttag ccatctcttt tccttttaga aatggtcttt
      361 ctaatccggt atcaattctt gcttgatcaa tagcctcttt tgtcatttca aaaacataat
      421 ttttcaattg attcgtttgt tgtttatcta acattactgt aattcctgca tccattattt
      481 ttctcctctc attttctatt tcttcaatca gccaactcgc caagttgtat ttatttagat
      541 tttcaaatca ttaagtaacc aatttaccaa cttttgataa gtagatgctc taatactttc
      601 tttgctccca ttttcgattt tgacatatgt tttccgattt atacctatca attttgctgc
      661 atcgttttta gtaagtataa gatcggctct atgtcttctt aagcttctaa tgaattgatc
      721 attaagagtt atcattcagc gcctcctttc aaattattca ctttgtggtt tttatcttaa
      781 taagcacatt ttaaaccaca aaacgagtaa tatcaattaa aatattactc aatttgtggt
      841 tttcatgcta taatagtttg gaatttacta tagggggcgt tactttgaat agaataaaat
      901 tttatattga ttccagtaaa aagaccatta aacaaattag tacagaaact ggtattaact
      961 atacaactct tacgaactat taccgtggtg agcgaaatcc tagaaatact gaaacatgga
     1021 atatccttgc tgattatttc catgttcctg taagttattt gatgggatta gacgaaacaa
     1081 attccataga acaacctaat cttgatagaa taaaaactgc tcgtttagaa aaaaaggaca
     1141 aaatagtcaa gaatattgcc agtcttgact cagattattt tactgaatta tctaatctat
     1201 acttttccat gacttcttta cttgaagtca aacttcaaga agatgactcc gactttcttt
     1261 ttttaaaaag tattctagag gactcctttg aatacacgat tctatttgcc aaacaaaaga
     1321 agtttattaa taaagcttta aacttaaata tcgatgaaag aaaagagtta gaatctgagt
     1381 tatttggcaa cttaacccaa gtacaggtta acaatctaaa aagagagata acagctcgat
     1441 taatatcaaa acttcaataa aactaattat tcaatcagcc aactcgccaa gttatcagat
     1501 tggagagatt agttatgtca atacaaaagt acacaacaaa aagcggtgca cgatatagtg
     1561 tccgcttgta tgttcgtaaa gatgaaaatg gaaagcaaga atactatgta aaaaaagggt
     1621 ttaaaactga aaaagaagcc aagttacacg aagcaagaaa aaaggtcgag attgaagata
     1681 ccggctatca taagccttct aatgacttgt ttataacagt gtatgaagaa tggttgcctt
     1741 cttataggaa tcgtgtacaa gaaacgacat atcaacgtac aaaagacctt ttccgcttgc
     1801 atatactacc aaaattcggg aataagtcca tttctaagat tactcctgtt cactgtcaga
     1861 atgcagtcaa tgaatgggct ttaaaattca agaatatcaa acagcttaag tcttacagct
     1921 ctcaaatatt tgagtatgcc atttttgcag agctattaca ccgaaatcct atggcaaata
     1981 caaatctacc aaagcgggaa aagaaagaaa atgaaaatta cttttcatta gaagaactaa
     2041 aagctttttg ggatattctc aataaagaag agcctttaaa acatatttta atcttccagc
     2101 ttttgattac tactggtata cgtaaaggag agctagctgc tcttcattgg tctgacattt
     2161 attttgaaga acagctaatg tatgttagaa agagctatgc aactattcga aatgaagaga
     2221 agaatgcaag aagaaaaact gtcaggattc aaaaaaatac aaaaaatata tcttccgaac
     2281 gaatattacc aattgatgca caaactattg aaatgttaaa aatctggaaa aaagaacaag
     2341 ctcgagagct tttgcagttt ggcataaata caaatacaaa aaatcaactt atctttacct
     2401 atgtaaatgc agacggtgac attaatcaac ctttgcatgc ggactattca aataatatat
     2461 tgaaacgttt agagaagaaa tataaattta agcacgttac tattcatgga ttccggcaca
     2521 ctcacgctac tcttctacta gaaggtggag caagtatcaa agaaacacaa gatcgattgg
     2581 gtcataaaaa tgctgaaact acccttaaca cttattctca tgttactgaa aaagctcaac
     2641 gaaatgcgtt agaaaaattc attgcttaca ctggtttata atattgtcat tacctttctc
     2701 attaccagaa cgaaaaaaag acaccttctc gcaatgagaa agtgtctgaa atcgttggta
     2761 taacaacgat taacgttttg aaaactgtga agctttacgt gctttcttaa gacctggttt
     2821 tttcttcgta tcattgaata agtttgaaaa gccttgattt ataaaggttt ttccgtaaat
     2881 atattttgtt atatttgatt aaatttgaaa tgtttgtgtt ctaaaatgtg ttctaattta
     2941 aaggcagtat atttcgtatt ttatattaaa tttttaggct cattcattct caaatccatt
     3001 ctatttagtc aataaatatt ttaaaaagac tttataaaca aagatgttca agcgagcatc
     3061 ttattttttt gtgcctaatt tgaaaggaga acatttcatg tataaaaaac aaaaaaagaa
     3121 attatgtgta ttaactattt tgagtttggc tgttcaatca attgtaggag caattgctcc
     3181 tacgattgct tttgctgatg aaattaccca tccacaaacg gtaaccgtag aactagattt
     3241 agcacatcaa tatgctgttg aaggaacttt tagtgatggt cgtccaatgt cagaagtcac
     3301 tgttccgcat tatgcagtgt acaacggcgt taaacaagat attttctgtg ttgaaccagg
     3361 agtcccgatt tacaatgagt tcactcctgg atatgagaag aacccgttac cagatatgtc
     3421 cgataaagcc aaattagttt ccgttttatg gaaaaaagta ggtacagatg cagatactca
     3481 tattgtggca cagaagatga tctggcaaga agttaatggg tatacccttc actcaataaa
     3541 acgatcagat ggtagtgccg taaatatcgc agccattgag gcaaaaatca atcaagccat
     3601 tgccgattat cagaagaaac caagcttcca taatagtacg gctaaaacag tattagggca
     3661 atcgactact gtgacggata cgaataatct gaatttgtca gagttcgatg aggttgtgga
     3721 aaataccgca aacattgatt atcgtgtgaa tggcaatcag ttgataatca gcccaaatgc
     3781 caattcaaaa gaaagtggtg tgttgacgct taagaaatct gctggtaccg gaacacctgt
     3841 cgcctataaa aaggctggcc aacagacttt aatggccggg gcgatcgata agccaaacac
     3901 ttatacggtc aaaattgatg tggaaatgga gggttctttg aagattaaaa aagttgataa
     3961 agaatcgggt gatattatac caggaacggt tttccattta gactttggaa aaactttacc
     4021 cgcaaaagac gtgaccaccg ataaagaagg cattgcgacc ttagatgaga ttcctcatgg
     4081 tacaaaggta accattactg aaaaatcggt gccggcgcct tatacgattg ataccacacc
     4141 catgactgcc acaattaaag caggtgaaac catttcagtg acttcaaaaa atacgcgaga
     4201 aaaaggtcaa attattctgg ataaaaccgg aattgaaaca gggagtgatc tttggaacga
     4261 taattactct ttagcgggca atacgtttgc cattcgcaag gatagcccaa ctggtgaaat
     4321 cgtccaagaa atgaccacgg atgaaaaagg tcatgcagaa acaccaaaag agattgccaa
     4381 tgcgttggaa ttgggaacct actatgtgac tgaaaccaag gctagtcatg gctttgtgaa
     4441 tactttcaaa ccagtaaaag tcgaattgaa ttatgtcaat caaacagtgg ctcttgttac
     4501 cagtaacgta aaagggcaaa accaagaaat tactggtgaa accactttga caaaagaaga
     4561 caaagatacc ggtcatgaaa ctcaagggaa agctgagttt aaaagagctg aatacacctt
     4621 gtttactgtt aaggatggaa aagcggtcaa atggaccgag aactttaaac cagaattgtt
     4681 gaagggaacg aaagcttctg atgaaaccgt aactttgact ttagatgaaa agaatcaagt
     4741 tgccgttaaa cacttagcga ttaatgagta ttactggcaa gaaaccaaag cgcctgaagg
     4801 atataccttg gatgaaacaa agtatcctgt atccatcaaa aaggtggatg atagcgaaaa
     4861 aaatgctgtg attactcgag atgttgcggc aaaagaacaa gtgattcgct ttggctttga
     4921 tttctttaaa tttgctggat caactactgg cactgccgaa actggcttca atgatctaac
     4981 ctttaaagtg tcgccattgg aaggaaccaa tgaaatcacc ggtgccgaag atgaagcgac
     5041 cacagcttat aacgagcaat tagggtttga tggttatggc aagtttgaaa atcttcctta
     5101 tggggattat ttacttgaag aagtagaagc cccagaaggt tttcaaaaga ttacgccatt
     5161 agaaatccgt tctactttta aagaaaataa agaggattat accaagagtg attatatctt
     5221 tactattacc gaaaaagggc aaaagcaacc gattaaaacg gtaacggtcc cttatgggaa
     5281 tctaaccaat gagaatttct cagtcagttt gaaccgtttg atgctttatg atttgcctga
     5341 ggaagaagat agcttaactt ctcttgcgac ttggaaagaa ggagataaag aattgactac
     5401 tcttgattct accgagctag tcgataaatt gagctataat ctacatgaaa tcaaagaaga
     5461 ctggtatatc gtagctcaag ccatcgatgt agatgctaca aaagctgccc aagaaaaaga
     5521 taaaaaagcc aaaccggtgg cgattgcgga aacaaccgca actttagcga ataaagagaa
     5581 aacaggaact tgggagattc tgcataaatt aactgtcgaa caagttttag acaagaccat
     5641 tgtcttattc aactatgtgt atgaaagcaa agaagcctat gaagctggtg atgagccggt
     5701 agcgaaggat gctagcttga acaatcaagc gcaaactgtt aattgtatgg tggaacgtca
     5761 tgtttccatc caaaccaaag cgcacttaga agaaggttct cagaccttta ctcatggtga
     5821 cgtggtagat atgtttgatg atgtatccat tacccatgat gtactagatg gctcaaaaga
     5881 agcttttgaa acaattctgt acgctttatt tccagatggt acaagtaaag aaatttggaa
     5941 atctggcaag attgattacg aagtgaatga caaagaattt accaaaaccg tacttgcgga
     6001 aaaagtggat acaagtaaat atccagaagg tacaagtttt accttcaaag aaattaacta
     6061 tgacaaagat gggaatatca atggaaaaca caatgaagat ttgaaagaaa aatctcaaac
     6121 tttaacgccc aaaaaagttc cgacaacacc aagtacacca gaacaatctg aaatgccaac
     6181 tgttccaagt ggctctcaag aatctagtcc tacagtgaag gcattccctc aaaccgggga
     6241 gaaaacttcc aatgtgctac tgttcattgg ctttagcttg atctttgcga cggctggcta
     6301 ttatttttgg aatcgtcgga actaaggtga taagatgaaa catgtaaata gtagaaaaca
     6361 atacgcgcga ccgcctccta aaaaagtggt cgcgctattt ttgtattcaa aactaacaat
     6421 gaaaagagga aaaaatatgg aattgaaatt tgttgtacca gatatggtcg agacgttcgg
     6481 aaaactaaca tacggtggtg aaggtgaagt tctatcggaa gggtatggtc gaaatactac
     6541 aattgttggt cgaagttacc atctgttttc aaataaacaa cgggccgatg atattgaagt
     6601 ggtggtagct gcggaagccg gcgaaaagga ttttgaccaa gatcaaccac taaaagctgt
     6661 aaatccccac ttaattgcca aaggctatga aattgagaat cgtgggttta ccgattacgt
     6721 gttgtatgtt gatgatttag tgaaagcata ggaggaaaag aagatgagat taacagaagg
     6781 tattgtcgta gattcaggat taacctttgg caagttgcgt ttttccgcat tacgtcgaga
     6841 agtacgcaag caaaatgagg acggaacggt tagtaacgag gtaaaagaac ggacctataa
     6901 tttgaaatcc tccgcgcaag gtcgaatgat ccaagtcagt attccagcaa atgtaccctt
     6961 acgcgagttt gcttatgatg cagaggtaga gctggtcaat ccaatcgtgg atacggtcgc
     7021 caattatgtt tttcgggaag gaaccaccgt taattggttt atcaaggcag atgatttagt
     7081 cttgaaacga caactgaatc aaggaaaacc tacgaaccaa aatgaaggaa agaaataggt
     7141 gaagaatatg gaaattaatg ttgaacatat tttagattgt ctcgatcaat ttggcaaagg
     7201 tgagctaacg gaggaacaac tcactaacgc attaacctat gatgaaaaaa tgtttctgat
     7261 tatgcatcaa gggttactgg aagtaggtaa cgatgccaaa gaagactttg atgtactgga
     7321 ttggttaggg gaagaagagt ctttctttat ggttgtcgag gtaaatgaaa gtttatgtcg
     7381 gcaggcagaa tctgttttgg aagaaatcga tgtggagatg ccggatgcca tcgaaagatt
     7441 tttaaaacaa ttagtggaaa cgaaacaact tcccgtagta gtaaatgact agcataactt
     7501 tttgaaattg cccaatgaaa tcctattggg ctttttcgac tggaggtgga aaccatcaaa
     7561 atgtacaaag gacatcgcat acgcgcagga gatcaacact tgatttatca ctttgttctt
     7621 ggctggctaa tctttttgtt cattggttgg atgagcgtct tttatttaca agaacttaga
     7681 caatttgata tttctaaggt atcattttcc actagtgaaa ttggctggtc tatgaaggat
     7741 gtaatctgct tgctaggaag tctcgctctt tcaggaagca tgatgttact ttatatacac
     7801 ttttttcagg atcattggcg aagtctttgg catcgacaaa agttagctcg gatgattcta
     7861 gaaaaccatt ggtatgaagt aaagcagact caaggtgaag gttttttcaa agatttgaat
     7921 agtagttgga ccaaagagag tatcagttac ttccccaaaa tttattatcg aatgaaagat
     7981 ggcttacttt ctattcgcgt tcaaatttca ctgggaaaat atcaggatca gctattgaag
     8041 ttggaaaata agttagaaag cggactgtat tgtgagttag tagaaaaaga actcaaagac
     8101 tcctatgtgg aatacacctt gttatacgat atgattgcca atcgaattgg gatagaagaa
     8161 atagtggcag aaaatggggc gttgcgattg atgaaaaatc aagtatgggc gtatgattcc
     8221 ttgccgcata tgttaattgc tggcggcaca ggtggtggga agacctattt cttactcacc
     8281 attattcaag cactattgaa gtcagatgca gaattgttta tcctcgatcc caaaaatgcg
     8341 gacctcgccg atttaggtac gataatgcct cacgtatact cccaaaaaga agaaatttct
     8401 gaatgtgtgg aagattttta cgaacgcatg atagctcgta gtaggtcaat gaaagaaatg
     8461 cccaactaca aaacaggaga gaattatgcg tatctcggac ttccgcccaa ctttttaatc
     8521 tttgatgaat acgtggctta tatgggtgcg aatcgttttc ctacaagcat cgaatagatg
     8581 tgaaatggaa gcctattgag attatcaata gtgacaacta atgttttgag agatggaatg
     8641 aaagccttca agttgagctg aaacatcccc gctaatactc ctacatgctc taatcaatga
     8701 agtagattgg ttttggtatg aagctaggtg aagtcgtctg aaatctgcct aagtgcttga
     8761 gcataaggct gactataggg cgggcggctg aaaaagataa ctatggtaag aatgtctgaa
     8821 aatgactgac gaatctgcga atgtacgggt ctaaaaggct gatcttattg aaagatagga
     8881 acatacggac gtatggtgag ataccgacaa gtaaggttcc atgatatgaa aatcgggata
     8941 gctaacgaag aagctataat ttcacaggct catagtagat acctaaggtt atacatgata
     9001 gctaaaaaca ttggaacgat gtaagcagaa aactatcaat taaatgacta cttctagata
     9061 ggtgcatata agtaataacg aaattgcttt gtttctgtga aagtaagggc acagtaccga
     9121 tgaagcgtct aacaaatgtg gagggatagc cctaagtcta tttaataaca atgaatgttg
     9181 aaacagatga agttcaagga tctggtaaga acgtgggaaa atccgttatg gaggtgccac
     9241 gcttgaacac aaaaatacgt tactgggaat attatgatct acaagaaaca ttcgatcagc
     9301 tatttactca aagtagaaac gggaaaaagt tttatcaatt atatgagtta atcatttctg
     9361 aaaataatat tcttttagca taccgtacta tcaaggcaaa caaggggtct tcgacaccag
     9421 gaacggattc cttcacaatt gataactata aggaaatgaa ccaagcagag tttatccatc
     9481 ttattctcag tcatttagaa aattacaaac caaaatccat aaagcgagta atgataccta
     9541 aacccaatgg tgagaaacgc ccattaggaa ttccatgcat gattgatcga atgatccaac
     9601 agatgttcaa gcagatactg gagccaatat gtgaagctaa gttttatgaa catagctacg
     9661 gtttcagacc attgagaagt gcaaagcatg ctctaggtcg cattatgtat ctcataaaca
     9721 tcagtaaaat gcactatgca gtagacattg atatcaaagg tttctttgat aatgtaaacc
     9781 accgtttact gataaaacaa ttatggaata taggaatttg tgacaaacaa gtcttagcta
     9841 ttctttcaaa atctctgaaa tcacccattc aaggagaagg tatcccgagc aaaggaacga
     9901 tccaaggagg tatcatttct ccactattat ctaatgttgt tctaaacgac ttagaccatt
     9961 gggtctcaaa gcagtggcat acctttgaaa caaagtatcc ctatactaaa ggttacaata
    10021 aatttcgtgc tctaagagat acaaatctaa agcaaggcta tattgtcagg tacgcagatg
    10081 actttaaaat catgactaat gattatccga cagcattaaa atggttccac gctgtcaagc
    10141 tttacctaaa agacagactt aaattagata tttccaatga gaagtctaaa atcgtcaatt
    10201 tgcgtaaatg taagtcggaa tttttaggat tcgctatttg cgtgaaacaa aaagggaaaa
    10261 aatgggtttg taattcacgt atttctaaca aaaaaaagga tcagattaaa gaagagatta
    10321 aacaaagaat caaagatatt caaaagagtc ctacagcaca aaatgctctc ctgtttaatg
    10381 ctctcgtgct cggtgttcat aattatttca ggtatgccac tcatattaat ttagatttga
    10441 ataggatgaa ctacgttctt tcattcacta cttacaaccg attatcaaat tgtagtacaa
    10501 gatgttaccc caacaaagct tccccaactt acaaaaagtt ttactcaaca acgatgaaaa
    10561 cgtataaagt agctggagtc tatttgtacc ctctttgcga cgtatctaca aaaacaattt
    10621 acggatttaa tacggaggat acaccgttta ctccgtttgg tcgtcaaaga ttggagcaca
    10681 aaagcctaca gtctcttgtg ttccaagaat taagaaaatt gatggaatcg aaaataccga
    10741 atagaacggt tgagtattta gatagtagaa tatctcgtta ctcaatgaaa tcaggcaagt
    10801 gtgagattac aaaacaattt ttgccagcaa aagccgttca ctgtcatcac tatttgccaa
    10861 aaagtcttgg tggagacgac aaattcgata acttgagaat tattcataag gatatccatc
    10921 ttctgatcca tacaacaaat aaaatgataa tcgatcacta cgtgcatgaa ctgaaactgg
    10981 tgccagagca aatagcaaaa atcaaccttt atcgaaagat gtgtaatctg caaaacattc
    11041 aatagaaaat aaatagatgg aacgccgtat gaggtgaaag tctcacgtac ggtgtggagc
    11101 gggggaaaag atggagatta tatcaaattc ttacctatcg ctatagatgt taacgacaaa
    11161 agaaagtgcg gtgattttga ataagcttaa acaaatcgtg atgttaggcc gtcaatctgg
    11221 tttctttctg attctagctt gtcaaaggcc ggatgcgaaa tatctaggag atgggattcg
    11281 cgatcagttt aacttccgtg tggctttggg tcgtatgagt gagcttgggt attccatgat
    11341 gtttggagaa gtcgataaaa atttttttat gaaacgcatc aaaggacgag gttatgtaga
    11401 tacagggggc agtgtgatta gtgagtttta tacgccactt gtaccaaaag ggtatgattt
    11461 cttaagggaa attagcaaca ttgttggatt aaccgttcat acggaaagag aaaataatag
    11521 tgtgtagcct aggaatatca ataatcctgg gcttttttat tgaggtgatt tatgacattt
    11581 ttagatttat tcgccgggat tggtggcttt cgcttaggta tggaacaagc cggtcaccag
    11641 tgtatcggtt tttgtgaaat tgatgaattt gctcggagga gttataaagc aatccatgat
    11701 acaaggaagg aggtggaaat gcatgacatc acaagtgtat cagacgaatt tgttcgatcc
    11761 ctcggaccag tggacatcct ttgcggcggc tttccgtgcc aagctttttc aattgcggga
    11821 aagcggcaag gattttccga tactcgaggt actttattct ttgaaatcgc tcggttcgcc
    11881 actcttctcc aacctaagtt tctattcctt gagaacgtcc ggggattgct caatcacgaa
    11941 ggaggggcta cgttcgagac gatcctccga acgttggatg gattggggta tgatgtggaa
    12001 tggcaagtgc ttaactcaaa ggcctacgtt ccccaaagcc gtgagcggat cttccttatc
    12061 ggacattctc gagatgcatg taccgaacaa gtatttccta tcattggatc gtcttcaaca
    12121 tctgatcaaa acatcagaaa cttggtaaat atcaatcctt ccaatcgagg aatgggtggc
    12181 caagtctatg gttcagatag cgtagcacca accttgaccg gtgatgaagg aattaaaatt
    12241 gccttaccgg taaaggatgg gatttctgta gcgggtatgt tgtcgggaaa ttttgaacaa
    12301 ggcaatagga tttatgaggt aaccggaaca gcccccactt tgtcaacgaa acaaggggga
    12361 acgaagatta tgattcggaa aaatgaaaca gaatatcaag aggtgaagcc aggggacagt
    12421 gtgaacctgg cttttcctaa ttccacaagt cgccgaggtc gtctgggaaa acaaagtgtg
    12481 cataccttac taacaggaga ccaacaagcg gtagtcacgg atcagtacca gattcgaaag
    12541 ttaacaccta gggagtgctg gcggcttcag ggctttccag attgggcttt tgatcgagct
    12601 tctcaagtca attcagatag tcagttgtat aaacaggccg gaaactcagt aacagttccg
    12661 gtcatttttg atattgctag aagattaaag ggggtaaaag catatgattt atgacgagtt
    12721 gattggagaa atctactgga tgattggcaa gattcaggca gatccgaaat tggaagaaga
    12781 actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccagg tgattcgtat
    12841 gtagtggatg aggaaacgga tgctcgaatg gagctcaatc aagtcatcgg tgaattggaa
    12901 cgaattgcgg accttgccaa agaacctgat atccgccaat acttgtttga gataaaagca
    12961 gagttggaaa ttgagggtat caccgcggag taatagggaa ataacccact gataggcact
    13021 gaccgcgggg gatttgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    13081 ggcagccggc catgatgtct caccccccgt atctaacagg ggggtacaaa aacacaaaaa
    13141 acaagcaagt aaccataaaa aatgcttgtt gtatcaagga tattaagtaa tttgtatagg
    13201 tgttaactca tttggaaaag ttaacacctt gaatttggag gtgaaaactt ggcgcaacga
    13261 aatttagatt atcggttatt aaaagaacga cgcaacgaat atggcgtttc acaaaataaa
    13321 ttagctactg cttgcggact tagccgccca tacctaaatc agatagaaaa cggtggcgta
    13381 atcgcctcta caaagaccat gagaaaaatt tttgatcaac tggaaagttt caatcccgat
    13441 ttacctttaa cgttactatt tgactatgta aggattcgct ttcccacaac ggatgtgcgg
    13501 aaaattattc aagaaattct ccatttgaaa ttcgagtata tgcttcatga agattacgcc
    13561 ttttactcct atcaagaaca atatgtcatg ggcgatattg tagtgatgtt atctcatgaa
    13621 gaagataaag gtgtcctttt ggaattaaaa ggtcgtggtt gccggcagtt tgaaactttt
    13681 ttactcgccc aaaagcgtag ctggtacgac ttttttgaag attgtctaaa agccggtggc
    13741 gtgatgaaac gcttggattt agcaatcaat gatcgagtgg ggctactgga tattcctgat
    13801 ttaacgaaaa aatgtcagaa ggaagaatgt atctccttgt ttcgtacctt caaaagttac
    13861 cgttccgggg aactgttgaa agctgatgaa aaggatggta tgggcaatac cttatatatt
    13921 ggcagcctca aaagcgaggt ttatttttgt ttgtacgaaa aagattatga acaatatatc
    13981 aagttaggga ttccattgga ccagaccgaa acgaaaaatc gttttgagat tcggttgaaa
    14041 aatgaccgtg cttattatgc gattcaagat ttgttaaaag gtcgtagcat tgaaagtacc
    14101 acgttttcca ttatcaatcg gtatttgcga tttgcggata aagtagatgg aaaacggcga
    14161 accaattggc ccttgaatga acaatggggt cgttttattg gacggaatcg gaaagagatt
    14221 caattaactt ctgaaccgaa accgtatacc attgaacgaa cattaaattg gctaggtaga
    14281 caagtcgccc ctacgtggaa aatggctaag gagttggatc gattgaatca aacaacgtat
    14341 atccaggata tggtacagaa tgcgcgactc tcggaccggc ataaaaagat tttggagcaa
    14401 caaagtatgg caatcgaaaa cttgattgta tgaaaagagg aattttgatg gaaattgtaa
    14461 aagatatgtt attggtactt ggtggcagta tgcttggtgt taccgtgatg tgcttgatga
    14521 atgcaagtgc agcagcggat tgagcaatgg aaaagaaagg aaagtgacaa aatgaatttc
    14581 ggacaaaatt t
//
LOCUS       DAZKOR010000070        14553 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000070 DAZKOR010000000
VERSION     DAZKOR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.071"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1114)
                     /locus_tag="ACG8ZM_002039"
     CDS             complement(227..1114)
                     /locus_tag="ACG8ZM_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0492979.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="ACG8ZM_002040"
     CDS             complement(1111..1980)
                     /locus_tag="ACG8ZM_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0492980.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="ACG8ZM_002041"
     CDS             complement(2117..3403)
                     /locus_tag="ACG8ZM_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0492981.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="ACG8ZM_002042"
     CDS             3676..4254
                     /locus_tag="ACG8ZM_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0492982.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="ACG8ZM_002043"
     CDS             4254..5183
                     /locus_tag="ACG8ZM_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0492983.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="ACG8ZM_002044"
     CDS             5180..6916
                     /locus_tag="ACG8ZM_002044"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0492984.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACG8ZM_002045"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACG8ZM_002045"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0492985.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="ACG8ZM_002046"
     CDS             complement(7812..8321)
                     /locus_tag="ACG8ZM_002046"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0492986.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACG8ZM_002047"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACG8ZM_002047"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0492987.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="ACG8ZM_002048"
     CDS             complement(9622..10713)
                     /locus_tag="ACG8ZM_002048"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0492988.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACG8ZM_002049"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACG8ZM_002049"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0492989.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACG8ZM_002050"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACG8ZM_002050"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0492990.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACG8ZM_002051"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACG8ZM_002051"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0492991.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4153 a         3156 c         2623 g         4621 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt ttt
//
LOCUS       DAZKOR010000071        14449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000071 DAZKOR010000000
VERSION     DAZKOR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.072"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(358..570)
                     /locus_tag="ACG8ZM_002052"
     CDS             complement(358..570)
                     /locus_tag="ACG8ZM_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492992.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
     gene            complement(591..1442)
                     /locus_tag="ACG8ZM_002053"
     CDS             complement(591..1442)
                     /locus_tag="ACG8ZM_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492993.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            complement(1543..1839)
                     /locus_tag="ACG8ZM_002054"
     CDS             complement(1543..1839)
                     /locus_tag="ACG8ZM_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0492994.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(1974..3467)
                     /locus_tag="ACG8ZM_002055"
     CDS             complement(1974..3467)
                     /locus_tag="ACG8ZM_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0492995.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4079..5032
                     /locus_tag="ACG8ZM_002056"
     CDS             4079..5032
                     /locus_tag="ACG8ZM_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0492996.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            5004..5279
                     /gene="prgO"
                     /locus_tag="ACG8ZM_002057"
     CDS             5004..5279
                     /gene="prgO"
                     /locus_tag="ACG8ZM_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0492997.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6183..6353)
                     /locus_tag="ACG8ZM_002058"
     CDS             complement(6183..6353)
                     /locus_tag="ACG8ZM_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492998.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6350..6700)
                     /locus_tag="ACG8ZM_002059"
     CDS             complement(6350..6700)
                     /locus_tag="ACG8ZM_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0492999.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            complement(6693..8018)
                     /locus_tag="ACG8ZM_002060"
     CDS             complement(6693..8018)
                     /locus_tag="ACG8ZM_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0493000.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            complement(8305..9477)
                     /locus_tag="ACG8ZM_002061"
     CDS             complement(8305..9477)
                     /locus_tag="ACG8ZM_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0493001.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            9629..10324
                     /gene="vanR-A"
                     /locus_tag="ACG8ZM_002062"
     CDS             9629..10324
                     /gene="vanR-A"
                     /locus_tag="ACG8ZM_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0493002.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            10302..11456
                     /locus_tag="ACG8ZM_002063"
     CDS             10302..11456
                     /locus_tag="ACG8ZM_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0493003.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            11671..12639
                     /locus_tag="ACG8ZM_002064"
     CDS             11671..12639
                     /locus_tag="ACG8ZM_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0493004.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            12632..13663
                     /gene="vanA"
                     /locus_tag="ACG8ZM_002065"
     CDS             12632..13663
                     /gene="vanA"
                     /locus_tag="ACG8ZM_002065"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0493005.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            13669..14277
                     /locus_tag="ACG8ZM_002066"
     CDS             13669..14277
                     /locus_tag="ACG8ZM_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0493006.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(14357..>14449)
                     /locus_tag="ACG8ZM_002067"
     CDS             complement(14357..>14449)
                     /locus_tag="ACG8ZM_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0493007.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT         4411 a         2765 c         2669 g         4604 t
ORIGIN      
        1 ttcgtcacat cttcgatcaa tttctcttac gattttctca tccacatttc ttattcttac
       61 ttccatatta attcctccaa tttaaaaagg atagcgaaaa ggagcctatc ctttttgcta
      121 tcctactacc ctccttttac tatccttcac tatcctttca ctatcctatt tttaaatatt
      181 ttgttcccac atttattttt gagaaacgta ataggcacag cctattggaa cacttttttt
      241 aaaaagtgtt cccacgtttc cttatgctct ttacaaataa aggcttttag agacgttctg
      301 gaattttaga ttccttgaat tgttcccatt catcaatcaa ttggctataa gtagccatta
      361 atttttttct tgctcttgtt gctttcgaat caacaaaaaa tcagtttgat tcgttaattc
      421 taaatattct gttcggtatt caatgttcat cgtacccgtt ttactacata gtaactgatt
      481 atgacgttct ttcactttga ataactgctc cttctttaag gtttttaact ccatgccaac
      541 tataagcaca atcctgtata gaggaatcat tttcaaacga ttctccataa ttatttatct
      601 ttcattggaa gttctaaatc actactgttc caaaaaatgt atggtttgtt ttgacgaata
      661 ttacttccat ctaaccacaa ttttccgatg ccatatcgta ggcttgcttc gacttcataa
      721 aaattgtaga tctcttttct tcgatctatt gctttcattt cctggccatt ttttaaaata
      781 gatctgatgc ccttaacagt agattcatgg atactcgtgt gaacgatatc cgtattgatc
      841 aattcgtcat atcttccaaa atcaacattg tactcataac caagaagtgt cggaatcagg
      901 tcaatttcaa tttctttttc aattgaacgt ttaataagtt tcgtggatac atacccgaca
      961 aaaaagagaa taatacccac ttcaaacaaa tacatgtgtc catttacaaa gtgaataaac
     1021 gtcaataaaa aatccttcac tccttttgtt gttcttccag attgttcaat ttgttttatt
     1081 aaagtcgcta gaattgcatt tacaacaatt aaatcgaata ggcttttgaa aagttttcct
     1141 gctgttaatt tttgcctggt aaattgcatg actaattgag taaaaataga caatagcatg
     1201 acaaaaatca acgtcattag cactgtaaaa atgtctgatg ctgcaatcct attgggatca
     1261 tttatcaagt aataagccgt tccaaatact ccaatcgagt aaacggttaa atcaagaata
     1321 agtcgtgccc cgtaactaca aagttttcct aatggccata attgcaatac ccaaaatttc
     1381 tttcgtttgc tatttctttt cagaggaagc caggtgtgtt caatccagtc gtccgatttc
     1441 atttcgattt cttccctaaa ttctttcctt taacgttttc ctttgctact ttagcagcga
     1501 taggttgctc aatgaaccaa ttttttaagt aaataaaaac tactactgga tatacttctt
     1561 cctttttctt aactcgttag ctattttata ctcaatatac tcctgctcta acacacacaa
     1621 acaatcatat acctgatcca tagtctcccc agaagctaag gacaacccat gaagaaactc
     1681 aatcggcaaa gaagcaactg ttttttccct ggtaacccaa gaagacaaag tcccctgctt
     1741 aaaaccataa agctcacaga acacttccac agataatccc aaacgaccaa tgataaacac
     1801 attaacagga tgtgaataga cataattttt taacgacata caacttcctc catgatacat
     1861 aagactgcat acgcagttat aagtatacca cctacacgta gctaggcaaa gggagcaaca
     1921 cctacagaag tatcatagat atattgtata gggcaatttg caccttttat ttttcaacct
     1981 gtatctactt caaaaagatt cacttcttta agagggttca agggggttga taattgtcta
     2041 aatgtctctt ctactaagct ctcgttgagt ccaaatacct ctgtgatcct tgttgaataa
     2101 tctagtctct tttctttact tagttgaata accctcgcga acaacgtttt gactgaagca
     2161 atgatcattc cgccatttct tcctcgtgtt accttgtaga agactttatt ctcctctcgt
     2221 aactcattta agatcttatc aagtgttcgc ttgggcatcg ttagttgtga agccaattct
     2281 ttttttgaca ggttcaaata aggctcacta gcaagacttt tttcgcttag ataagccaat
     2341 agatcttctt tccattcatt tttatgacta tattttcttt gatctcgtgg ctttttaaat
     2401 ttccaccagc cacgtcgttg aatgaacaga tcattatttt ttaaggttgg tgaaacccat
     2461 tgctggcaca attcaataat gaattctctg cttgctgctt gataattacc agaataagca
     2521 ctacgtacaa tctttactaa ttctgcttca gatagcggtt cctgtaaccg ttcgttgaat
     2581 gtgaacatat tgtattcgca agtatccaat gcataacctg aagagtaata agctaaagaa
     2641 agcgtgaaaa tcacattatt tcgaccaaga cgtccttctc cgccgacgat gtttgcttta
     2701 tgaagtaaca tatcgaacca cggttcatct acttgacgaa tttctttttt ctcaggaaag
     2761 acaattagtt ttcgttctcg atcttctttt gttttttgat ctgattcaag cttcatcgac
     2821 cattgaatcc agtcagcaaa agaatactga tagttcattt ctttaaaaac aatattattt
     2881 tcttgtggga atcgagcaat tccgaagtgg ttacatccta gatccactcc ggacaatttt
     2941 tcagccaact gtagacgaat tgtttttgaa attttttttg ctacatcgat caccttgaaa
     3001 ttgcttttct ttgtgacata gcctggtgtc tttagtacaa aataaacttg ataaccatga
     3061 tctgattcta aaattagcgt tggcatgtag cccacttgat ctaaacaggc taaaataatt
     3121 tcgccttgat ggttctcttt tgaatcgata tcaactacaa atgtgttgat ttgacttaag
     3181 tttttctcag aatgaccttt aaccactgtt cgataatcat ccgcataagt accaaagcga
     3241 taggtattag gtgtccaatg cgttagtttc ttttcgttct ctaaaatagc ttcttccgaa
     3301 gtaatgacca taccacgccc aacattcatt aattgcttgc tacggtacgc aaagatagca
     3361 ccactcctac tggaatcaat atgacctgca ggtttcatgg tgctgtttcg atatttgtat
     3421 gtacgtaagc cgtttttaag aatagctgaa taaatttcgc tcaacatcga ttgtaacgtc
     3481 ctttctcaaa caaaaagaga acacagttta gctttaatct cagccatagt atgtgttctc
     3541 tcttcagtgt ttaagaatat ttaatttaag tacactaaat aaacgttctt ctgttgagtt
     3601 gctcgccttt ttttggtaga ataaaggcaa tcggaaaagg tatccgatca acttttaata
     3661 taagtagttg gtagctactt acatattctt gagacgagcg tgaacacatg tcctaaccat
     3721 gtgattcaga aataattgat tctgctaaaa tcaattattt ccgtctcttt tttttatgta
     3781 atttttctgt ttaaagagta ccactgcttt tgctactttg taaatagtga attatctgtg
     3841 gtttctatag aattaagttt acctcttttc actctaaatt gtcaacgcaa aacgcacaaa
     3901 tatattttga cattttgaat atatctgata tactgaatat agcaaatata tttagtatat
     3961 ccactatatt ccttataaag tctgaattct tatcaatttg gagtatatta gatatatcga
     4021 atatattaag tatatgaagt atactaaata tatttagtat aggaaattgg agggaactat
     4081 ggaaaactta cggactgcat ttaaaacatc agataaacta ggaaaagttg taatgcttac
     4141 aggtagaaaa ggcggcattg gtaagactac agacaatgat ttattagcga ttgtttctag
     4201 tcaacttttt gaaaaagacg tgttgctaat tgattatgac caacaaagaa atactacttc
     4261 caacattgga tcaacgtatc aaattactag ctttgatcga tccatgtctg cagcaatcaa
     4321 aaaaggggac tgggtttcag gtattactca agtaagtcca catttatata ttatggctgg
     4381 ctctcctgga tctgaagaac ttaatgaata cttgtccgaa aaatatcctg atcgtcgtaa
     4441 gagatcattg gccttcatta aacctttaga agaattgaga aaaaatttcg attatatctt
     4501 tatcgactgc ccaccatcaa cagataatgt ggttcgtgca ttcttaactg ctgcagatta
     4561 tattattgca atgcaagaat taaaacggta cgcaatggaa ggaacagaag attttattaa
     4621 taaagtttta gttccgattg tcacgaattt tgaagagtct catcttcaaa tcattggaat
     4681 tttgcctgtt cttttctcag tgcgacgttc ctctcaacac gcaaactatc aaaaaacaat
     4741 tgataagtat ggtgaatcaa acatttttaa aagcattgtt aaaggctcgg atcggttaga
     4801 aatgtatggt gagaatggta tccaattaaa tgattatgta gatcgaagat ggtgggctat
     4861 ttttgcagac atctttacag aattagaaga aagaattgaa tactatgaac aacatggtga
     4921 tattgaagga tttgattata cacctaaata tgctgactca atggccaatc atatcctacc
     4981 tcttggaaag gagattaaac taaatggcat tattaccaac gaacaaagat aagaagaaaa
     5041 ttggtgctga agatatttca attaaagatg atattaatgt cgagttaggg gaacgaaagt
     5101 acactgcgaa agaaagaaaa ccagttcaag tagatcctcc cgtactaaaa accattcggg
     5161 atcttagtta tgcaaaagat attcctatgt atgaaatagt caaacttgca atggacgcct
     5221 atgttgatac tttaaacgac gaggaacgga ttatatataa tagacgatca aaagattaaa
     5281 atatagtaag tatactagat atagtaaata tatttagtat acttactata tttttagctt
     5341 tattaatcct aatccttcaa aatatgtgta ttgtttgggg gtatacaaca ttcacaaatt
     5401 ttattgtttc tagtttttac tatactaaat atatttaata tagtgagtat agtaaatata
     5461 ttggttttta tctacaggta atggtggttt taacaaacat atagttacag ttactgaaga
     5521 acagtccgtt agtcagcagg agtaagtaat aaatcgtttg tgagacacta ttttgattga
     5581 tacgacctct aaccatgtgt ttgtttcatt tatgttataa taagaatgta agcatattaa
     5641 acagcaaact atcacagcag cactagactc aatcaaatga agggggatgg tgctaatgaa
     5701 aaaactacta gcaatttccc ttatcctaaa aaggagaatt aatagtgtca aaagaaccta
     5761 tgtccgcatg gatgcagctc gctcttagca tcataaagct gatctctaat tttgtggctt
     5821 tagacaatag agcaaaggtt ctaactacgc aatgtttagt aatacttact tgtagcctac
     5881 tggttgtaat agtatttctt ttacttgcgt tattcgtagt acaccttttg tttttttaaa
     5941 acaaaagaag cttattcaac tatatttagc tgaataagct ggtctcaatg ttaaatcaag
     6001 tttgttggac gggtttaaca ttgctttcta gtgctatttt agttagctct agtatgcttc
     6061 aagctagtta caacgtccta aggttgtaac tagttttttt atatttttta attacctata
     6121 ggtaatttta tcagaattga tacaaaatgt aaaacataaa agaaatataa ttttattgca
     6181 ctttaatagc caatatagta ttcgtattta tggtatcctg ctgcagcttc tacatagaaa
     6241 gagaagcgtt caacaccaac ctcatttatc aaacgctggc cataacccca aatctcttct
     6301 gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt cattctaatc
     6361 gaccccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag tgaactttgt
     6421 tttttccaac gtagatcccc agctcatcac tacctaagat cttgctaaca atatcttctg
     6481 gatagtatcc ttcagcgttt cgttcttctt tttgaataca tactgatcta ttttttatga
     6541 ctgcagcgtt caatacttcc gcaacctctt cagtcgtcat ttgttctttt gccaaatttt
     6601 gattggctcg ttgttcattg tcggcatcaa ttttttcagt atgatcagat aaataaaaac
     6661 ctgcccactt taaacgtcca cgatcattat aatcaatcat ttttaagccc tcctgcgccg
     6721 cctgcatggc caccaaccaa tgtacttcta gtaatactac gagcgccttc taacatagaa
     6781 gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat aattttatct
     6841 aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg aagcgaatca
     6901 gtgtacacaa gcttgctata ggttattcca acgtgcctta cttcttgtcc ttcataatac
     6961 ttatcaaaca agaaaagaca gtaattctgc aattcctttg tattatctgt tatagggatc
     7021 ttcatctggt gtgaaaaacc tggtctcgtt tctaagatcg acgtgccaat gtataaatgg
     7081 acacaacctg ttttacagtt atgttgcctg atacgaatag ctacttgttc cgccatttct
     7141 tttaccacca gttcgatttc atttctcctg gtataatctc gtggtaacac ttgtgaatta
     7201 ccatatgatt tttcctttgt cggttctggt gggatcgcta tatctgttcg atcgatcccg
     7261 ttcgcatgaa aatacagctg taagccaatc acacctaaat ggctcttaat agtgtatgga
     7321 ttccaattag ctaagtcttt aatactcatg ataccaattt ggttgagtct ttttttcatt
     7381 cgagatccaa taccccaaag gtctgtcatt tcaggaatat tccatacttt atcaggaaca
     7441 ttttgatacg tccattcagc aaggaatcca tcattatgtt ttgcttcatt gtctaaagca
     7501 agtttagcta ataagggatt atctccgaca cctactgtag caagcaatcc tagctcttct
     7561 ttaatattct cctggatcat ttgagccagc ttctttctcc gttgacttct tgttccttca
     7621 gtcgtaaaaa gattcattga tctggtcact tttaagatcg attcatcgat cgagtaaatc
     7681 aataaatctt cgtctgctac ataccttctg aaaatattgt ttacctgcat atttcgctta
     7741 atatagagat tcatgcgtgg tggaacgaca tgtaacgttt taggaaaagg ttgcggtaga
     7801 tctcgtggtc gactgacatt agaaataccg tacttcttct ttgcagctgg tgaagaagct
     7861 aaaatcaacc ctgatcctgt attatccgct cgactcatga cgactaattc agtcgttaat
     7921 ggatctaaat tcctttctat acattcgaca cttgcgtaaa agcttttcat atcgatcaaa
     7981 aagtaatcat ttactggctc ttttgaataa tccatcataa ataacactcc attcttccac
     8041 attatacaaa catacgttcg tttttgtaaa gaagaatcga ttaaatgtac aaaaaaagaa
     8101 gcataggtaa gttatttccc tacttcttct gttgatttgt aaaatcaaca cattgtatca
     8161 ctttattaaa tggatattta ctattagtct tgtgaaaatt tgaaaataag tgtcgtatta
     8221 tccagttttg cttagttata ttagaaaaaa ataagtcaag tccataatcc tgtgtaaaat
     8281 actatacaat gttttaccgg tctctcattg atcaaactta atatattttc ttgtagaact
     8341 gagccattcg tcatgaaggt ccataaggac agctccaatt aatcgattgg cagacgttcg
     8401 gttgggaaaa atccgaataa tcttttctct tctgcggact tcctggttca gtcgttcaag
     8461 aaggttggtg ctttttagcc gattatgact atttccgata accgtgtatt gaaaggcatc
     8521 ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt cgatatattc
     8581 accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact taaagatcgc
     8641 ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga agatgtttct
     8701 taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac gaatcgcaga
     8761 cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc cgcgttcttt
     8821 taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa tcatgaagcc
     8881 aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc ttttagaaag
     8941 cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat aaggataatt
     9001 cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact gctcagtcag
     9061 gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa tctttgaaac
     9121 tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca gtgccttttc
     9181 atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg ttctaggcac
     9241 ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc catttctttg
     9301 actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg ttcgttgatt
     9361 ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat cttttaccga
     9421 atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt gggtcatgta
     9481 acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg gctgttacat
     9541 ggccttttat cttttacaca attatatgga ctttatccta taatctgata aattattaag
     9601 ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg tggatgatga
     9661 acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata cggttttcaa
     9721 atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg accttgccat
     9781 attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa taagggacaa
     9841 gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata aaattacagg
     9901 gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg agttaattgc
     9961 tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg agcagaacga
    10021 aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt gttatctgaa
    10081 cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc tctgtgaaaa
    10141 caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg acgaatattt
    10201 cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa aaatgaacga
    10261 caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata aaattgaaaa
    10321 ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc gttgcaattg
    10381 ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg aaacttgggg
    10441 attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac gcgatgaaat
    10501 tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg attgtcatta
    10561 gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt gacgagataa
    10621 ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt tctgcggaaa
    10681 tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag cgagagcagg
    10741 atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg cacgatatta
    10801 aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct ccagacatgc
    10861 cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat cgactcgaac
    10921 agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata acgctaacaa
    10981 aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt tatcctcagc
    11041 tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc gtgtccggcg
    11101 accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct gcatacagtg
    11161 aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg tcaatcgaat
    11221 tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa aagttctata
    11281 ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg gcgattgcaa
    11341 aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat aactatacga
    11401 cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg tcctaagaga
    11461 tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag gaaattaaca
    11521 atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta agaacgagcc
    11581 gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg ttttatttgg
    11641 tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac tgtttatgga
    11701 tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg cgttatggca
    11761 acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt caatcaatgt
    11821 atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct gaagagagcc
    11881 ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac aactgctgct
    11941 aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt tgccgattat
    12001 actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg ctctgtggaa
    12061 aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat gacagttggt
    12121 gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg atttggatgt
    12181 aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc gtttgatgag
    12241 ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga tacgcactat
    12301 attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat caatactggg
    12361 cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg gaaactgggc
    12421 ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga ttgcacccaa
    12481 aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt gataatcaca
    12541 ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa aaccattaaa
    12601 aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg caatactgtt
    12661 tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga tagccgctaa
    12721 cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg gtgtatggaa
    12781 aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag ctgtactctc
    12841 gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg aaatcaacca
    12901 tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat ccatacaagg
    12961 tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct cagcaatttg
    13021 tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta ctcccgcctt
    13081 ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc ctgtttttgt
    13141 taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg cggacgaatt
    13201 ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg agcaggctgt
    13261 ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag ttgttggcga
    13321 ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag tcgagccgga
    13381 aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag aggagcgagg
    13441 acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag gtctagcccg
    13501 tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca atactctgcc
    13561 cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta ttgcacttcc
    13621 cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat ggaaatagga
    13681 tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata tgccacttgg
    13741 gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt agggacatac
    13801 gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg gtacggattg
    13861 cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca atgggctgca
    13921 cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg aactgagatg
    13981 atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc cattgatctt
    14041 acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt tgattttatg
    14101 gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca aaatcgcaga
    14161 cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga atggtggcac
    14221 tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt taaataaact
    14281 tttaaccgtt gcacggacaa acggttctgt tgcaaagttt taaataaaga ataaaatccc
    14341 ttacggtatc tatgatttaa gctgggattc ccaataatac cttgatttca gtacagaccg
    14401 aaaacccgaa gagagtgcct tcttttcggg ttttcttata taatcctcg
//
LOCUS       DAZKOR010000072        13897 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000072 DAZKOR010000000
VERSION     DAZKOR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.073"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG8ZM_002068"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG8ZM_002068"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0493008.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACG8ZM_002069"
     CDS             complement(2260..2511)
                     /locus_tag="ACG8ZM_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0493009.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACG8ZM_002070"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACG8ZM_002070"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0493010.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACG8ZM_002071"
     CDS             3461..3679
                     /locus_tag="ACG8ZM_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493011.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="ACG8ZM_002072"
     CDS             complement(3968..4480)
                     /locus_tag="ACG8ZM_002072"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0493012.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACG8ZM_002073"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACG8ZM_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0493013.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="ACG8ZM_002074"
     CDS             complement(6933..7376)
                     /locus_tag="ACG8ZM_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0493014.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="ACG8ZM_002075"
     CDS             complement(7377..8165)
                     /locus_tag="ACG8ZM_002075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0493015.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACG8ZM_002076"
     CDS             complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACG8ZM_002076"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0493016.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="ACG8ZM_002077"
     CDS             complement(9280..9867)
                     /locus_tag="ACG8ZM_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0493017.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACG8ZM_002078"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACG8ZM_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0493018.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="ACG8ZM_002079"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="ACG8ZM_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0493019.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="ACG8ZM_002080"
     CDS             complement(12784..13719)
                     /locus_tag="ACG8ZM_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0493020.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3848 a         3106 c         2452 g         4491 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgta
//
LOCUS       DAZKOR010000073        13771 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000073 DAZKOR010000000
VERSION     DAZKOR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.074"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..389
                     /gene="yaaA"
                     /locus_tag="ACG8ZM_002081"
     CDS             147..389
                     /gene="yaaA"
                     /locus_tag="ACG8ZM_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0493021.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            376..1500
                     /gene="recF"
                     /locus_tag="ACG8ZM_002082"
     CDS             376..1500
                     /gene="recF"
                     /locus_tag="ACG8ZM_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0493022.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1497..3443
                     /gene="gyrB"
                     /locus_tag="ACG8ZM_002083"
     CDS             1497..3443
                     /gene="gyrB"
                     /locus_tag="ACG8ZM_002083"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0493023.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3463..5934
                     /gene="gyrA"
                     /locus_tag="ACG8ZM_002084"
     CDS             3463..5934
                     /gene="gyrA"
                     /locus_tag="ACG8ZM_002084"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0493024.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6170..6469
                     /gene="rpsF"
                     /locus_tag="ACG8ZM_002085"
     CDS             6170..6469
                     /gene="rpsF"
                     /locus_tag="ACG8ZM_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0493025.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6519..7031
                     /gene="ssb"
                     /locus_tag="ACG8ZM_002086"
     CDS             6519..7031
                     /gene="ssb"
                     /locus_tag="ACG8ZM_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0493026.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7057..7293
                     /gene="rpsR"
                     /locus_tag="ACG8ZM_002087"
     CDS             7057..7293
                     /gene="rpsR"
                     /locus_tag="ACG8ZM_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0493027.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7436..9409
                     /locus_tag="ACG8ZM_002088"
     CDS             7436..9409
                     /locus_tag="ACG8ZM_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0493028.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9415..9867
                     /gene="rplI"
                     /locus_tag="ACG8ZM_002089"
     CDS             9415..9867
                     /gene="rplI"
                     /locus_tag="ACG8ZM_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0493029.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10141..11508
                     /gene="dnaB"
                     /locus_tag="ACG8ZM_002090"
     CDS             10141..11508
                     /gene="dnaB"
                     /locus_tag="ACG8ZM_002090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0493030.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12288..13580
                     /locus_tag="ACG8ZM_002091"
     CDS             12288..13580
                     /locus_tag="ACG8ZM_002091"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0493031.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4583 a         2480 c         3026 g         3682 t
ORIGIN      
        1 tttaaggctt tctgtttatt tttgtttaaa tcaccttttt tagattaaat cgtcttatat
       61 ggaaaaatat atggataaat ttgttttatt gcgaaaaaaa aggtataata gagtatactt
      121 ttaataatga aaatgaggga tgggcattga aacaacaagt cattctaaat acggaattca
      181 tgacgttggg ccaaatgttg aaagaagtca cagttatcgg tagtggcgga caagcaaaat
      241 ggtatttggc agaaaacact gtgttagtcg atggcgaacc agaaaatcgt cgtggacgca
      301 aactttatcc cggcatgatg gttgaagttc cagaagtagg aacttttttt atggtaaaaa
      361 aaggaaacga cgataatgag gctgaataag ctgtatttaa aaaattatcg aaattacgaa
      421 gagctgaaca tcgaattttc caagaacctc gtcatctttt taggagaaaa tgcgcaagga
      481 aaaacgaatc ttttggaaag tatctacgta ttagctatga cgcgaagtca tcggacaagt
      541 agtgagcagg aattgattgg ctggactgac gatcaggcga tgattcaagg agagatcaca
      601 aaaggttcat ccactcttcc attagaaatc ttattatcta aaaaaggccg aaaaaccaag
      661 atcaaccata tcgaacaaaa aaagttaagt acttacgtgg gacaaatgaa tgtcatttta
      721 tttgcaccag aagatctttc tttagtaaaa ggcagcccgc agatcaggcg gaagttttta
      781 gatatggaat taggccagat caatccaatc tatctttatg atcttgtcca ataccaagca
      841 attttgaaac aaagaaacca gtatttaaag caattgaatg aaaaaaaaca aactgatttg
      901 ctttatttgg atattttgtc cgaacaacta gcagcgtttg gcagcaaagt attgaaagca
      961 agagcacagt tcgtcaagcg attggagttt tgggctaatt ctttgcatca gcaaatcacc
     1021 catcaaaaag agcagctgga aatcgagtat ctcaccgcag tcgattcttt agaaacacat
     1081 acgcaagagc aaatccaaga acaatttctc gcacttttga accaaaataa aaagaaagac
     1141 ctttttcggg gaacaacgac agttggtcct catcgtgacg atctttcctt ttttattaat
     1201 cagaaaaatg tgcagactta tggatcgcaa ggacagcaaa gaacaactgc actaagtgtc
     1261 aaattagcag aaatcgattt gattaaagaa gagacggggg aatatccaat ccttttgtta
     1321 gatgatgtga tgagcgagct ggatgatagt cgtcaactcc atctgctaga aacaatcgaa
     1381 ggaaaagttc aaacgttctt aacaacaacg acacttgacc atgtaaaaaa taaaatgact
     1441 gttgaacctg agatttttta tgttcaacaa gggaaactaa aaggagaaga aactgaatga
     1501 cagaagaaag aagtttagta gaacgcgcta aagagtatga tgccagtcag attcaagtac
     1561 ttgaaggact tgaagccgtt cgtaaacgac ctggcatgta tatcggttcg actagttctg
     1621 agggtctgca tcaccttgtt tgggaaattg tcgataattc gatcgatgaa gtattagcag
     1681 gttttgcaac gaaaatccat gtgattattg aaaaagacaa cagtatcacc gttattgacg
     1741 acggtcgggg gatacctgta gatatccagg caaaaactgg acgtcctgca gttgaaacgg
     1801 tctttactgt tttacatgct ggaggaaaat tcggcggtgg tgggtacaaa gtttctggtg
     1861 gacttcacgg agtaggatct tctgttgtta acgcgctttc tacacagctt gatgtcaaag
     1921 tatacaaaga aggaaatgtg tactatcaag aatatcgtcg tggagctgta gtggacgact
     1981 taaagattat cgaacaaacg gatcgccatg gaacaacggt tcactttacg ccagaccctg
     2041 aaatttttac agagacaact gagtttgatt tttcaaaatt ggctacccgg atccgtgaac
     2101 ttgctttctt gaaccgcggg atgcgtatat cgattgaaga caaacgagaa gaagaaccag
     2161 tcatcaatga ataccattat gacggcggga ttaaaagtta tgtcgaatac ttgaatgcaa
     2221 acaaaaccgt gatttttcca gaacctgtat atttggaagg tgaacagcaa gatatcgcag
     2281 ttgaagtctc tatgcaatat acagacggct accattccaa tatcatgagt tttgccaaca
     2341 atatccatac gtatgaaggc ggaacacatg aatcaggatt caagactgct ttaactcgtg
     2401 tgataaatga ttatgcccga aaacaaaagc tgatgaaaga aaatgacgac aacttgaccg
     2461 gtgaagacgt gcgagaagga ttgacagctg ttatttctat caagcatcct gaccctcaat
     2521 tcgaaggaca aacaaaaaca aaattgggca attccgaagt acggacagtc actgatcggt
     2581 tatttagcga acatttcatg aaattcttgc tggaaaaccc aagtgtgggc cgtcaaattg
     2641 ttgaaaaagg attactagct tccagagctc gtctagcagc gaaacgtgcg cgtgaagtaa
     2701 cgagacgtaa aggtgcttta gagattagta atctgccagg aaaattagcg gactgctcta
     2761 gcaacgatcc tgaaaaatgc gaattgttca tcgtcgaagg agattcggcc ggcggatcag
     2821 ctaagcaagg tcgtaatcga gagtttcaag ccatcttgcc tattcgtggg aaaatcttga
     2881 atgttgaaaa agcaagcatg gacaagatct tggcaaatga agagatccgg tctttattta
     2941 ctgctatggg tacagggttt ggagaagatt ttgatgtatc gaaagctcgc taccacaaat
     3001 tagttatcat gactgatgcc gatgtcgacg gcgcgcatat ccgtacattg ctgctgactc
     3061 ttttttatcg ttacatgcgt cctattgttg aagctggata cgtctatatt gctcagccac
     3121 ctttatacgg ggtaaaacaa gggaagaata ttacgtatgt acaaccagga aaaaatgcag
     3181 aagaagagtt gaaacaagtc gttgcttcac ttcctgcctc accaaaacca agcgttcagc
     3241 gttataaagg acttggagaa atggatgatc atcaactatg ggaaacaact atggacccaa
     3301 gcaatcggat gatggcccgc gtcagtgtgg acgatgctat tgcagcagat cagatatttg
     3361 aaatgttgat gggggatcga gtagaacccc gtcgtgcatt catcgaggaa aatgcacact
     3421 atgtgaagaa cttagatatt taatcatgaa ggaggaaaaa gaatgagtga agaaatcaga
     3481 gaaaacatcc atgatgtcaa tctaaccagc gaaatgaaag actccttcat cgattatgcg
     3541 atgagtgtta ttgtcgcacg tgcacttcct gatgtcagag atggtctgaa acccgttcat
     3601 cgccgtattt tatacggcat gaacgaatta ggtgtaacac cggataaagc ccataaaaaa
     3661 tctgccagaa tcgtcggaga tgtcatgggt aaataccatc ctcatggaga ctatgcgatc
     3721 tatgagtcaa tggtacggat ggcacagcca ttcagctacc gttatatgct agtggatggt
     3781 catggaaact ttggttctgt tgacggtgac ggtgccgccg ctatgcgtta taccgaagca
     3841 cggatgagca aaatcgctac agaaatgctg cgtgatatca ataagaacac tgttgatttc
     3901 caaagcaact atgatgatac agaaaaagaa ccagttgtac tacctgctcg cttcccgaat
     3961 cttttagtga acggaacaac ggggatcgcc gtaggtatgg ccacgaatat cccacctcat
     4021 aacttgtctg aggtagtagc ggctatcgat ttgctgatgg ataatcctga tgtgacgacc
     4081 aatgaattga tggaagtatt acctggacca gattttccaa caggtggttt ggttatggga
     4141 aaatcaggaa tccgaagagc gtatgaaaca ggtaagggtt ctattaccgt acgtgcaaaa
     4201 gttgagatta ctgagatgcc aaatggtaaa gaacggatca tcgtgacaga attgccttat
     4261 atggtcaata aagcaaaatt gatcgaacgg atttctgaac tgcacagaga caagcgtatc
     4321 gaagggatca cagatttgcg ggacgaatct tcccgtgaag gtatgcggat cgtcattgac
     4381 atccgtcgtg acgccagcgc atctgttatc ttgaataact tatataaatt gacatccttg
     4441 caaaattcat ttggattcaa tatgttggcg atcgaaaaag gtgtgccgaa agttttgagc
     4501 ttgaaacaaa ttcttgaaaa ctatatcgaa catcagcgcg aagtcatcac aagacggaca
     4561 gtctttgaaa aagaaaaagc agaagcaagg gcacacattt tagaaggttt gcgtattgct
     4621 ttggatcata tcgatgaaat cattgcgatt atccgcgggt ctaaatcaga tgacgaagca
     4681 aaagcgacga tgatcgaacg gttcgattta tccgatcgcc agtctcaagc tattttagat
     4741 atgcgtttac gccgattgac cggtttggaa cgggaaaaaa tcgaaaatga ataccaagaa
     4801 ctcttaaaac tgattgaaga tttaactgac attctggcaa gaccagaacg tgttacagaa
     4861 atcatcaaga atgaattagc agacttgaat caacgatttg gtgataaacg acgcacagaa
     4921 ttactagtcg gtgaggtctt aagtctggaa gatgaagatt taatcgaaga agaagaaatc
     4981 gttattacat tgacaaacaa tggttacatc aaacgtttgg caaataatga attcagagct
     5041 caacgaaggg gtggacgtgg tgtccaagga atgggtatcc acaatgacga cttcgtcaaa
     5101 aatctggttt cttgttctac ccatgataca ttgttattct ttacgaataa cggaaaagtg
     5161 tatcgggcaa aaggatacga aattccagaa tacgggcgca cagccaaagg aataccaatt
     5221 atcaatttac taggtatcga ttcgagtgaa agcatccagg caatcattgc ggtgacggga
     5281 gaagcagaag aaggccacta tctcttcttt acgacgcgtc aaggtacagt aaaacgtact
     5341 tctgtcactg cttttgctaa catccgaagt aatggtctga tcgcaattgg tttgaaagaa
     5401 gacgatgagc tcgtcaatgt gctactgaca gatggtcaat ctaatatcat tattggaaca
     5461 cataacggct actctgtcac tttcaaagaa gaagcagtcc gcgatatggg acgtacagct
     5521 tccggggtta gaggaatacg cctgcgtgag gaagattatg tcgttggggc agccttaatg
     5581 aaggaagatc aagaagtatt agtgatcaca gaaaaaggtt acggaaaacg gacaaaagta
     5641 tcggaatatc cagtgaaagg ccgcggaggt aaagggatca aaacggcgaa catcactgaa
     5701 aaaaatggtc cactagctgg attgaccact gtttctggag atgaagacat catgctgatt
     5761 acagataaag gtgtcattat ccgtttcaac gtatcgactg tttctcaaac tggacgttca
     5821 acattaggcg tacgtttgat gaagatggaa gctgatacga aagttgtcac gatggcagca
     5881 gtcgaaccag aagctgcaga acaaacagtt gaagaacaag gatcagaaga atagccatca
     5941 agactaattg tgtaagctag caagaatgac cgattaaaaa atctggttat tcctgctggc
     6001 ttttttgatt agttgaattt ctcttaaaat attcgaaact gttcattctg tattgctttt
     6061 gaccaaaata gagtgtataa tcagagcttg tgagtagtct aattaattag ataactctcc
     6121 ttgctcttag gaacctaaga gccgaaagtc cacagggagg tgaatatcaa tggaaaacac
     6181 gaaatatgaa atcatgtaca ttattcgtcc aaacattgat gaagaagcaa aaactgcttt
     6241 aatcgaacgt ttcgatgcaa tcttgaaaga taacggagca gaagttatcg aatcaaaaga
     6301 ttgggaaaaa cgccgtttcg cttatgaaat gaacggttac cgtgaaggta tctatcacat
     6361 cgttaaagta tcttctccat catcagcaaa tgctgtcaat gaatttgacc gtcttgctaa
     6421 aatcaacgac gatatcattc gtcacatgat tgttaaagaa gaagcttaat tgttgtttca
     6481 tgtgaaacaa tagaaaacga tgagaggaga tgactatttt gattaataat gttgtattag
     6541 ttggacgtct gacgaaagat ccagatttgc gttacacagc aagtggtacg gcagtcgcaa
     6601 catttacttt ggctgtgaat cgtaatttta caaaccaaag cggcaatcgc gaggctgatt
     6661 ttatcaactg tgtgatttgg cgcaaatcag cagaaacttt agcaaactat gctcgtaaag
     6721 gaacactttt aggtgttact ggacgtatcc aaactcgttc ttatgataat cagcaaggac
     6781 aacgtgtata cgtgacagaa gtagtagctg acaacttcca attgctggaa agtcgttctg
     6841 catctgaaaa tcgccaacaa agcggcggat tccaaagttc tggtcaatca gctggcggat
     6901 ttggcggaaa caataactct aatcaaacat ctcaatcatc caacggtatg cctgatttcg
     6961 accgtgatac ttctgaccca ttcggctcaa gttcaacgat cgacatttca gacgatgatt
     7021 taccattcta aacgaatttg ataggaggga aatagaatgg cacaacaaag aagaggcgga
     7081 cgtaaacgtc gtaaagtaga ctacattgct gctaaccata ttgaatatat cgattataaa
     7141 gatatcgaat tactaaaaag atttatttca gaacgcggta aaatcttgcc acgtcgtgta
     7201 actggtacag gcgctaaaaa ccaacgtaaa attactgttg caatcaaacg cgctcgtatc
     7261 atgggcttgc tacctttcgt tagcgaagac taataaatcc taggaattga atgactgatc
     7321 cttttgatca gctattcaat tccttttttt ctgtaaaaat aacctctatt cgtttagtcg
     7381 ctcgaatgac gaaagttatg gtaaaatcaa attagtcata tatgggagga aaattatgga
     7441 aaataaaaac aatcatttat ccccgaaacc tctcttcctt atcctgttta tattaatcga
     7501 agtattggta gtcttaattt taccatttcg ttttgttagc gcttcggtca ttctattgat
     7561 gaatttatgg ggtgttcgct attggttgaa aatgaaacgt tggcttattt taaataaacg
     7621 tgaaaaaatc cagcaggctt cgactgtagc tgaaaccaat ctagcttatg tctcagaagt
     7681 catgcctgta ggagtgattg cttatcagcc atcaagccaa aaaatcgaat ggctcaatcc
     7741 attagcattg aaaatcaaag atcaaagtgg attaacagag gaagaattgc tagatacttt
     7801 ttttactgca aaagaaaaaa agaaaaacca agtctatcta gcagataaaa catttcagtt
     7861 tgatatggat acagtaaagg gtgttgttta ttttaatgat attacccaaa accagttgtt
     7921 aaaactccat cagacgaaca gtcagccggt gattggtatt ttatctattg ataactatgc
     7981 agatgccatt gataaattgg atgacaaaga gatttcttat ttgaactctt tcgtcactac
     8041 actagtttct gactggatga atgaatacca tgtctttttc aaaagaatca acgcagaacg
     8101 gtttttcttc gtagcacaga tgatcgatct gaaaaagatg atggataaaa aatttgattt
     8161 gctggatcgc tttcgagcag aagctgaaaa acagagctcg cctatcaccc ttagtatggg
     8221 gattgcttat ggctcaagaa ccctcgatga aatcggtgga gaagctcaga ataatttaga
     8281 tgttgcttta gtccgtggag gcgaccaagt tgtcgtgaaa gaggtaaaag aaggagcaaa
     8341 acctgtttat tatgggggga aatctgctaa gaatgtcaaa cgcacgcgtg ttcgttctcg
     8401 agcgatgagt accgctttgc gtcggatttt tgatgaaaca ggcgatgtgt ttatcatggg
     8461 gcacaagttt ccggatatgg atgctttagg ctctgctttt ggtgtcagtt gtctggcacg
     8521 cttcaaccag aaaaaagctt acatcgttat caacgagaac gagatcattc cagatgtaga
     8581 acggtgtctt gaagaaatcc ataagcatcc tgaattagaa atgcaactaa tctctccaga
     8641 acgtgccata gagctgaaaa aagaagagga cctcctcgtg atggtggatt accataaacc
     8701 ttctctatcg atctctcagg gcctatatga gcaatttgat aagattgtga tcattgatca
     8761 ccatcgtcgt ggggaagagt ttccaagtaa acctctgctg acttatatcg aatcttcagc
     8821 ttcttcagct tcagagctag tttctgagct gatccaatac caaagcagca aaacaaatcg
     8881 cttagatcgt tttgaggcca cattattgct tgcagggatg gttgtagata caaatagttt
     8941 caatgtccgt acaactgcac gaacttttga cgttgccagc tatttaaggt cttgtggagc
     9001 agacgcatcc ttagtacaat atttattgag ctcggattta gcgacatact tggagatcag
     9061 caatttgatt gctggaagcg actatgtgac ttctgatatt gttgtggcgg cagggacaga
     9121 cgaaaaagag tataatagtg ttatagcagc aaaaacagca gatacattgc tttcgatgat
     9181 caatatcaat gcagctttcg tcattacaaa gcggacagac ggcttgattg ggatcagtgc
     9241 cagaagtacc ggcacgatca acgtccaaat catcatggaa tccttaggtg gcggaggaca
     9301 cttcacaaac gcagctactc aactggaaaa tacgacagtt gaggaaacaa aacagaagct
     9361 atttgctgca atcaaagaaa atatgaatca aatatatgat aaggagtaat ccaaatgaaa
     9421 gtaatttttt tacaagatgt aaaaggaaaa ggtaaaaaag gggacatcaa agaagttcca
     9481 gcaggttatg cacaaaatta tctaatcaaa aataacttgg caaaagaagc aacgaaaagc
     9541 agtatcagtc agttagcagg acaaaagaaa gcacaagaaa aacacgaagc tgaaatcttg
     9601 gaagaagcta aaaagttaaa agaattcttg gaaaaagaag atactgtagt cgaattgaag
     9661 gcgaaagcag gggaagacag ccgtttgttt ggttcgatcc cttcaaaaca aattgctgaa
     9721 gggttaaaca aacaatacaa aatcaaatta gataaacgaa aaatcgaatt agaaaatccg
     9781 atccgttcac taggctacac aaacgtacct gttaagcttc atcatgaggt gacggctacg
     9841 atcaaagttc atgtcgtaca agaataacaa atcttgatag cgatgttcag ctggtaagta
     9901 taaccagaag gaaaaaagac aaaccgaaat ttatctgaga aaacagctgc agcaatgaaa
     9961 gcaaaagctg caaatcgatt tcagattgat ttcggtttct tttttgggaa cagcaaaata
    10021 gcataaagaa aacgagtata ataaaagaga actatttttt tattacactt tcggtacgaa
    10081 tgatgacttt tctctcattt tcttgtaaaa tgaagcaatt gaagacaagg agcgatacgt
    10141 atgaatgaag tgtggcagga tcgaatccca ccgcaaaata ttgaagctga acaagcggtt
    10201 ttaggctctg tttttcttga cgcagaaacg atcattgatg ccatggagta catagagcca
    10261 aaagactttt atcgtcgtgg ccatcaaatt atttttgaga caatgatcga gttaaatgat
    10321 cgtaatgaag cgattgacgt ggttactgtc aaagaccggc tggaacaagc caatttgtta
    10381 gaagatgccg gtggtttaag ttatctctca gacttggcat tagcagttcc gacagcagca
    10441 aatatcgtct actatgcgaa aatcgttgaa gaaaaatcat tgctgcgtac attgatccag
    10501 acagcaacag gaatcgttac gaaaggattc gagcaaggag aagaagttca aacaatttta
    10561 gatgatgccg aacgcagtat cttggaggtt tcggaaaaaa gaaatcgaag cggcttttta
    10621 tctattgctg aagtactgaa ctccacgatt gaaaatatcg atcgactagc tcaaaacaat
    10681 gaagaaatca caggtctacc aactggctat caaggtctgg ataaaatgac agcaggtttc
    10741 cagcccgaag aactaatcat tcttgctgca cgtccagcgg taggtaaaac agcgtttgca
    10801 ttgaatattg ctcaaaatgt aggaacaaaa acagatcgtt ctgttgcgat atttagtttg
    10861 gaaatggggg cagaatctct tgtaaatcgg atgctttgtg cagaaggttc gattgaggcc
    10921 agccatttac gtacaggtca gctttcggaa gaagaatggt cgaatctgat cgttgcgatg
    10981 gggagtcttt ctcgagcaaa tatttttatc gatgatacac cagggatcaa aatctctgaa
    11041 atccgtgcga aatgtcggaa actagcgcaa gaaaaaggca atctaggctt gatattgatc
    11101 gattatttgc agttgattga aggtaacggt aaagagaacc gtcaacaaga agtatcagat
    11161 atctctcgcc aactgaaaaa actggcgaaa gagctgaaag tacctgtcat tgctctttca
    11221 cagttatctc gtggagtaga acagcgacaa gacaaacgac cagtgctaag tgatattcgt
    11281 gaatccggat caattgaaca agatgcggac atcgtagcct tcttgtatcg agatgattat
    11341 tatgaaagag aaggcggaga tgacgatgat ggaaacgagc caaaaaatga caacgtcatc
    11401 gaagtcatca tcgagaagaa ccgaagcggt gcacgaggaa cagttgaact actgtttatc
    11461 aaagaataca ataagttttc gtcgctttcg ccaagagaag aattttaaag aagaggttgt
    11521 gagccttgag caatcccgag agctctcgta agaagatgca aggagtaacc gcaggaacga
    11581 ctagagtact taatttaaac agatctccaa agggattgtg acaaaagtct tgtcacagtc
    11641 cctttttccg aataaacggt gttcaatcag ctgacccttg gtattagctc agcaaacgtc
    11701 aaaatatgag gaactatttt gacgtttgtt tctctaatac tcaggtcaaa acgctgatct
    11761 tacgacctct tttccttaaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata
    11821 tcatcaaaca tgagaagcag ttttttgaaa ttgctccttg tgactcagag ttaaacggca
    11881 ggctcacaac ctctttctta aacggtgttc aaaagcttgc atctgcggta atacgcccaa
    11941 tcaaacgaaa aatatgagaa gctatttttg tttggctgtt cttattactt gatgcaggac
    12001 agctttttca caacctctga ttcacggtgt tccaaaagta gcttcttcga aaaataagtc
    12061 gaaatttcac aaaaatttga gaagcaattt tcgtacattc cttcttattt cttgaagctg
    12121 accacttctg ttacaacctc ttttttaata accattctaa aaagataaag ttcgtttttt
    12181 aagcgttttt ataaaataaa aataaaaatc attcgatttt atttgaaata tgtatctatt
    12241 attgatacaa tggttcagga ttaaataata tagaacgagg tgttcacatg tcatctgtag
    12301 tagtagtagg tacgcaatgg ggcgatgaag ggaaaggaaa aatcacagac tttctcagtg
    12361 aaaatgctga agtgattgca cggtaccaag gtggcgacaa tgcagggcac acaattaagt
    12421 ttaacggtgt gacttataaa ctgcatttga ttccttctgg gattttttat aaagacaaga
    12481 tcagtgtgat cggaaacggc gttgtagtca atcctaaatc attggttaaa gaattagctt
    12541 atttacatga aaaaggtgta gaaacaagta atttgcgtat ttctgaccgc gcgcacgtaa
    12601 tcttgcctta tcatatcaaa ttggaccaat tacaagaaga tgctaagggc gaaaataaga
    12661 ttggtacaac gattaaagga atcggacctg cctatatgga taaagcagct cgtgtaggta
    12721 tccgtattgc agacttatta gataaagaaa tttttgaaga acgtcttcgt ttgaatctag
    12781 atgacaaaaa ccgtcaattt gtaaaaatgt ttgattcaga accacttgat tttgacgata
    12841 tttttgaaga atattatgaa tacggacaac aaatcaaaca atacgtcaca gatacttccg
    12901 ttatcttgaa tgatgctttg gatgcaggca aacgcgtgtt gtttgaaggc gcacaaggtg
    12961 taatgctgga tatcgaccag ggaacttatc cttttgttac ttcttctaac ccagtagccg
    13021 gtggggtaac aatcggaagc ggtgtagggc cttcaaaaat caataaagtt gtcggcgtct
    13081 gcaaagccta tacgtcacgt gtcggtgatg gaccatttcc aactgaatta tttgatgaaa
    13141 caggagaaca aatccgcgaa gtaggacgag aatacggtac gacaactggt cgtccgcgtc
    13201 gtgtaggctg gtttgattct gtagttatgc gccattctaa acgagtttca ggtattacca
    13261 acctatcttt aaactcgatc gatgtattaa gtggattgga aacagtaaaa atttgtacgg
    13321 cttatgaatt agatggcgaa ctaatctacc actatccagc aagcttgaaa gagttgaacc
    13381 gctgcaaacc aatctatgaa gaattgccag gctggtcaga agacattaca ggatgcaaaa
    13441 cacttgcaga actgccagaa aacgcacgta actatgtacg tcgtatctca gagcttgttg
    13501 gtgtacgtat ctcaacattt tctgtcggac ctgatagaac acaaacaaac atcttagaaa
    13561 gcgtctgggc gcaaatctaa ggataaaaac gacgactgaa tcaggacgtt cttaccagag
    13621 cgagtcgaag tgtttatcta tacgataata ataacttgaa gaggtagtga aagagacgtt
    13681 tagccttatt gccgataggc tgtctcttga acactgttta ttcagaataa gggactgtga
    13741 caaaaaccgt gtcacagtcc ctttttccga a
//
LOCUS       DAZKOR010000074        13715 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000074 DAZKOR010000000
VERSION     DAZKOR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.075"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(204..1346)
                     /locus_tag="ACG8ZM_002092"
     CDS             complement(204..1346)
                     /locus_tag="ACG8ZM_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0493032.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(1351..1536)
                     /locus_tag="ACG8ZM_002093"
     CDS             complement(1351..1536)
                     /locus_tag="ACG8ZM_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0493033.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(1890..2135)
                     /locus_tag="ACG8ZM_002094"
     CDS             complement(1890..2135)
                     /locus_tag="ACG8ZM_002094"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493034.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(2129..2530)
                     /locus_tag="ACG8ZM_002095"
     CDS             complement(2129..2530)
                     /locus_tag="ACG8ZM_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0493035.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(2717..4525)
                     /gene="uidA"
                     /locus_tag="ACG8ZM_002096"
     CDS             complement(2717..4525)
                     /gene="uidA"
                     /locus_tag="ACG8ZM_002096"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0493036.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(4548..6314)
                     /locus_tag="ACG8ZM_002097"
     CDS             complement(4548..6314)
                     /locus_tag="ACG8ZM_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0493037.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(6327..7121)
                     /locus_tag="ACG8ZM_002098"
     CDS             complement(6327..7121)
                     /locus_tag="ACG8ZM_002098"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0493038.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(7131..8507)
                     /locus_tag="ACG8ZM_002099"
     CDS             complement(7131..8507)
                     /locus_tag="ACG8ZM_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0493039.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(8627..9274)
                     /locus_tag="ACG8ZM_002100"
     CDS             complement(8627..9274)
                     /locus_tag="ACG8ZM_002100"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0493040.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(9358..10785)
                     /gene="uxaC"
                     /locus_tag="ACG8ZM_002101"
     CDS             complement(9358..10785)
                     /gene="uxaC"
                     /locus_tag="ACG8ZM_002101"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0493041.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(10859..11938)
                     /gene="uxuA"
                     /locus_tag="ACG8ZM_002102"
     CDS             complement(10859..11938)
                     /gene="uxuA"
                     /locus_tag="ACG8ZM_002102"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0493042.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(11941..13572)
                     /locus_tag="ACG8ZM_002103"
     CDS             complement(11941..13572)
                     /locus_tag="ACG8ZM_002103"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0493043.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
BASE COUNT         4353 a         2742 c         1773 g         4847 t
ORIGIN      
        1 aaaaaccacc ggtggcaaac cggtggcaaa tccatgtaaa catggtttat tttttacact
       61 gttttcaaac aaacccttgc tatttcttcg gttcgtcctc gtccattttc gataccgaca
      121 gctagcttat aacccaaggt atatcaacat aaaaaaataa aaataggcta tgacagcagc
      181 gtgacagcaa aaaagatgga aagttaaata tcaaacctcg taatttcatt tgcgagtgct
      241 tgatttgttt ctggatataa gtgtccatat ttgtccaaag tagttttaat agaggcatga
      301 cctagacgct tagatattgc atagatattt ttattatgtt caattaaaaa ggcaacatgc
      361 gagtgtctta atccatgcat atgaattggt tttactcctg caatttcagc tagttttttt
      421 attctgctta aaatagttct tggagatggc ggtaatccat caaaagaaaa aaccaattct
      481 atatttttcc caattgattg ttgagttttt ttccatgttt ttaaatcttc aacggtttgt
      541 ctgtctaacg aaattatccg aatacttgat gtattttttg tttcagcaaa ttcataatct
      601 tgtcttgtac ggatgtaaat tgacttattt acgctacatc tgccatttat taagtcaaag
      661 tctttccatt gtaacgctaa gagctcctca ctccttacac ctgtaacaaa aataaaacgc
      721 atcaaacgtt tataaaattc ttcataaaaa tccccgtgat aaacactatt atatactttc
      781 ttaaattcat caactgtcca aaattctact tgaggacgct gagtttttat tttacctata
      841 gtatctacag gattttcttc aataatttca agtacaattg cccttttaaa aactactctc
      901 aacttattga atatctgttt aatataattt ggtgataatg gttgctcaat gccctttttt
      961 acaacatgga ttttcgataa aaactgttga aattcctgta tatgaattgg tcgaatcttt
     1021 acaacctgca tttctccaaa aaaatccaat gcacgttgta aaatatgatc tgttttcata
     1081 tatgtttttt ctacagttcc caacttatac caaggaaaaa aatactctcg ataaaaatca
     1141 tgaaacgtta ttccttgcag ataaattcct tgtgaatcag aaaaactttg ttttaactct
     1201 tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc ttctggtcac ttgaattctt
     1261 tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc aagtgcctct ttttctgtct
     1321 ttataaacat tcgcaatttt cctcatttta ctattcctcc caagaaatat ggagaacttc
     1381 ttctaaaatt gaaataggaa ttctttttat ttttgaatta cgataaaaaa agtatccttt
     1441 ctcaactaaa agttctttac attccttgaa gattttatta gcatagtact gagaaaaacc
     1501 tagattaatc aaatcttttc gagtagctgt tttcattata gcaccttcct tacttttttt
     1561 catttatttc tatcagctgc ttttgtcaaa agcacatagg aatttcacct ctccccaagt
     1621 ttacggcgag ccttacggaa gtatcattat cccaacttta aatcatcgct ttttattgac
     1681 tataaaaatt attttagaat atttatcgct ccttctttac agcaagaaat catggcgcaa
     1741 ctaaaatatg ctccttaaag tgttaacgta ctgatagaat ctatttaatt ttcaatgaac
     1801 aataggtttt ctacctaaaa gaatctacta attccttaac tattagaaga ttcttttaaa
     1861 cagaaaaagt ggaaagggga tttttttaat tagttaattt taaattttaa gatcgcttct
     1921 attaatttaa tttctagctg acttatcata tattcatcta cataggtaac gaattgacct
     1981 acctcgttaa atccatttgt caaacataac ttagccatat aagaatgata atgtttgagt
     2041 atcatcttga tcgaaattgg atctccttct agagcagata agataatgga tagtggaact
     2101 aataggtact gccgcttttt agatttatct accataggtc ataccccatt ttcaattgtt
     2161 caactgcctt ctttcgttga taccagatac cacttgtgct catattgaga attttaccga
     2221 tttcagcatc attaaaacct gcaaaataat aaagtaggat aatctttctt cttatctccg
     2281 ataactgatc aattgctgct gataactttt gatctgaaat taatatttcc attccaaata
     2341 agaaatagac atcttgttca tcaaaatgag tttgggaata cctgccttca tttgaatctg
     2401 ccaatggttc ctcattatcc tttatgttag cgtttcgttt tcgaatagac ttagcttcgt
     2461 ttcgtaatac ttttttacag taggcatcaa attggtgttg tatagatatg tcattgtaat
     2521 gttgttgcat gataatatgc tcctttttcc ccctttccac taatacaaca atcagaaagc
     2581 cccttttttt cgaaaaaact agaaaaaaaa cataaaaata tttatcattc tttaaacaga
     2641 aaatctttct ataaaaaaca aaaaaaaacg attaggctag gctaatcgtt ttttgaacat
     2701 tcttaaaaaa tccaaattat tttttataac tgaaattggg tattgatgac catcgtttct
     2761 gtagatagta agcaatcatt ttaggttttc tatctctagt aaagattcct ttcttatttc
     2821 cttgcactct taaaatccct tgacttgttg caaaatctgc aaaattccac acttgttcac
     2881 caacaaaatt ttcacatgta tctattactt cgtgatttgc ttcgtaataa gccacttgat
     2941 actcttctgt aaacattgtt ggtactgtat catgaaatcc agctactgta tctgctccat
     3001 attctgtaaa cattattggc gtatcaggac atctttccat ccatccatct agctcttttt
     3061 tcaacagata ttttgcatgt gccagatccc ctcccgaaac ataccaacca taataacgat
     3121 ttaaacataa aacatcgaca atatctccta ccttacaaac atctggcgtt gattttagat
     3181 aagtaacgat tgttacaggt cttttctgcg gatcaagttc tttagttaat tcaactaaag
     3241 gttcaaaata ttctttagca ccttcggaat ccgaatctgg ttcatttgca atagaccaca
     3301 ttacaacact tgggtggttc ttgtcgcgtt ctatcaattc cttgatcacc gttttatgag
     3361 aattttctgt tcgtatctct ttccacgtat tatgtttttc cacattatca tctaataaag
     3421 tggccatgaa atttaaatgt aaccctactg caggtacttc atcaataact acaatacctt
     3481 ctcgatctgc taatcgcata atttcttctg agtaaggata atgtgatgtt ctaaaagaat
     3541 tagcacctat ccatttcatt aaattaaaat ctttaatatt tacagcctca ttcaaaccac
     3601 gtccattaac atacgaatcc tcatgcttac caaatccttt aaaataaaaa ggtttattat
     3661 taatcaaaaa tttgccattg gtaacctgaa cactgcgaat accaaattct tctgtatagg
     3721 tatcgatcac tttattatca tcaattaaac taattttcat actatataaa taagcgtcta
     3781 gaggttccca aaaaattgga ttcttaagct gaatcattcc tttctttcct tcagcttcag
     3841 ctacaagttg agcctcttcg tcatatatcg ctattaagat ttttccggtc cattctgtat
     3901 cgatattgaa ttctacactt gcatccaaat cagttattct tgtattaata gtaatatcac
     3961 ttatatagtc tttcggagtt gtatataact taacaggtct atgaatacct gcataattga
     4021 agaaatcaaa attaactaag ttttcctttt ttatatcccc attttcctgt actgtttcct
     4081 tcataatccc cacaggtaaa gtactatcgt caataatatt gtttacagca actgtaattc
     4141 gattattccc gtcttttaca aaccgattaa tttcagactc aaatggtgta aacccacctt
     4201 tgtgttgagt aataaacttt ccattcaaaa aaacttttgc ttcatgtgtt gcagatccaa
     4261 aacgaagaac taatctttct tctaataatc ttttaggaag agtcacttct ttttcatacc
     4321 acacccatcc aacatgattt ctaatgttct ctgtttcaaa taaatcatta taagaagagg
     4381 gaactgaaat cggataactc tctttttcat ttattggttc taaagcaagt tcttcctgta
     4441 agtcatcatt tgtatctaat ttaaatctcc agagaccgct taagtcaatt acttgcctcg
     4501 tctctgtcat aatgggatac aacatataaa ccactcctat ctaaatatta aataaatgta
     4561 tcctttaaac cacaataagc atctattgga cttactccct taaattcaga aagacccatt
     4621 agtttgtcca ccaatatttc taaagtttct tgttttccat cataagtatt aatataagtc
     4681 tttacttgag gtacatcagc caaatgaaac ggacactgta cagatacaaa aattgttgga
     4741 atttcatgaa tgtaaaacgg tatatccgga gttcctttac ttgctggcca gacaattcgt
     4801 tgaactgtgc ctggattaac attagctata ttaatgatca agtcatattt tttagttaaa
     4861 tttttaattg gtctcttagc agcatatatt ccagctatat ttttaatttt ttcttcttgc
     4921 gataatcctt tcaattcttc ttcaggtgaa atgtattttt caacttcaaa tccttttgct
     4981 gacaaaatct gtttcaataa atcaatggct tgaggtctat ttccagcaac tacttcacca
     5041 aacccacctt ttaccccatg tacatctact agtaaaatcc ttttatattt ttctggtaca
     5101 ataggccaaa tatcttgttt atctttaact aatgtaattg cttggtcagc cacctcttta
     5161 aaaattgatt tattttcaat taaaccaatt ttttccagag cttcttgctt aggtaataaa
     5221 atttgttctt ttgctttttt gtgtaatccc aatttagctt ttaaccctaa aattcttttt
     5281 aaggcatcta ataaccgatc atcagtgata attcctttac gatagccttc catcatataa
     5341 ttaaagtctt catctggatc attaaaaaat agaaatagat cacatccagc tgcaatagaa
     5401 gtgggcaata attctgatcg tttcattgcc ccagtcattc ctaacatatg actagcatca
     5461 gtaactacta atccattaaa tccgagtttt cctctaagta agtcagtgat taattctttt
     5521 gataaagttg caggaagtaa atcttcatct ttcagatcag gattaaaatg ttttgaataa
     5581 gctggcatat ggatatgacc tgccataatt gaaggtaatc cagcttctat taagcctgag
     5641 tatacttttc caaaagaatt atcccattct tccgtaccta aactattaat actaaaagat
     5701 agatgctgat ctcgttcgtc aataccgtca ccagggaaat gtttagctgc aggcaaaatt
     5761 ccactctcct gaataccttt catataagct aaactaagtt ctaaaacttt atcagggttt
     5821 tgtccaaaag atcgactaga aatgatagga tttctccaat ttctattaat atctacaata
     5881 ggagcaaaac tccagttaca tccaatagcc gatgcttcaa tcccagaaac tctccctaat
     5941 tcataagcat atttcgcatc atttgttgca ccaattttta cttctacacc aacttcagta
     6001 ccatctgtac aagctccgtt accacctgct tccgtattag cagcaattaa tagaggaatt
     6061 ttactttctg tttgtaagat atagttttga tcatatacct catcagcagt tcctggatta
     6121 tatcgtacag ctccaatatg gtactgattg accatattac tcaagtaatt tttatcacga
     6181 ctagatccca tattaacaaa caattgacct attttttctt ccaaagtcat agaatcaatc
     6241 gtctcttcaa tccaagaaat agcatcttca tctaaattat atggtttttt tcgtaaatct
     6301 acttttatag tcattttctt tctcctctac ttattagtaa gatttttcca aaaagtatct
     6361 atatattttt gttcaatatc tacataatct cccgataaac atttaaataa aacatcagtt
     6421 cttaagtttt cccatgattc tttttcttta ttgaccaaaa taggataaaa gaaaacttta
     6481 tttttatttg cagcttctag atcaccagga gaatcaccaa tcattaatat cttatccaaa
     6541 tcacaaccat tttcaattac acttcgaata atatcttctt tttttccttt atcttgacaa
     6601 cacagttcat caactaatga taataattca tgtctttccc attcttcttg aacagcttcc
     6661 ttattagctg agcttacaat aattatcttc ccatattttt tcagagcttt tatagtatct
     6721 ctagcactta aaaagggttt atctaatcct tttgcttgac taatctgttg attgacttct
     6781 tccgaccatt ttaaagcttt taataaatcc tctgactgag aatattctat ttccctcttc
     6841 aaagatggat tagacaattc ttttgttaga gatacccagt gctttaatcg attaatcgat
     6901 gttacatcat ttttttctaa tgttttaact aaacctaaaa atctgtttat tccacgagtc
     6961 tctgaatata aattaatatt attccaatcc tgtaggaatt tttctctatg atgaacatca
     7021 aaaatctctg cagcaattgg tccgaaaaag agattgtgtt tataagtcat cgtatccata
     7081 acacagccat ctgaatcaat acaaaatatc caattattca tatttctctc ctatttatgt
     7141 ttttgtttta aaagctcgca attttctaag actttatctt tagataatgg ataaaggaaa
     7201 aataaaatta atcctacaat cagaaaacaa attgatggaa ttaatgttga agcgttatat
     7261 aacttttcta aaacatctcc cgtttgtaca tttgctaaag agtcatagcc tataatagaa
     7321 agagtccaac ctccgacgcc tccagctaga gcttgaccta tttttctagc aaaagaatat
     7381 accgcatata cggtcccatc ttctctttga tcagacttta cttcttgatc atcaataaca
     7441 tcagtaatat ttgcccaaat aacagtatta aacacaccca aacccatata gccaaccgaa
     7501 ctaagaatta agaatacata catattacta gcttttatca aaaataagat caaatatact
     7561 attgctgaaa agaacattcc tactgatgcc agttcttttt ttccatattt tttacttaaa
     7621 aacaaagtta aaggcgaaac aactattaaa accaaaatag gattgataaa attcattaaa
     7681 actattccct gtgcatcatt atagtaatca ggaaatacat aattattcat actcataatt
     7741 aataattgag caaggagtaa gaaaatagcc gcaccaatta tagaaagtaa tgccctattt
     7801 ttaactaacc cttttaaaga ttttaacaat gaaactttgt attgagattg gtcaattctt
     7861 actcgttcag tagttagttt ataacatatt aaataacaaa taagtgcaca tatcgaaaat
     7921 attcccgcaa caattgtaaa agttgatcca ccacgaacta tttgagcgcc tgaatcatct
     7981 cttgtataaa taaataatgg tgttaataca ccaatgacca aacttgctaa agtagctcca
     8041 acatttcgga atgtcgatag cattgttctt tcttctgtaa tagaagtaat agctgatgcc
     8101 attgatccat aaggaatatt tatagatgtg taaaaaatac tcccccataa tatatacgta
     8161 acatacatgt atactacctt aacgctcata gaagctgaaa tcatagaagt ttgatacatt
     8221 aaaaaacttg ctaaagcgac tggaccactc attcttaaaa tccacggttt aaacttaccc
     8281 tgcttagttg ctttcgatct atcaacaata cgccccatag ttacatcagt aactgcatcg
     8341 ataaatcttg ctactaaaaa taatgttcca acggcagctc cgcttatccc caatactttt
     8401 gtataaaaga tcattaaaaa agaactagca aaaatgaaag taaaatcgtt tccaaaatct
     8461 ccaaacatat atcctatttt gtctcttata ccaaattttc tttccataca attctctccc
     8521 ttctgttgtt aagcgttttc attctgatga aagtacgtac tatactctga agcgtaaaga
     8581 gtcagtattt attttttaca tttcagagta ttatttgttt tttctcctaa taattcttta
     8641 tctcataaaa cttatctata tatttactag ctactcgact tacttcttca aaatcacctt
     8701 tttcaacaga agccaataaa ttcccaccta caccaactgc aacaactcct acttcaaacc
     8761 aatcttctaa gttctctata cttactccgc cagtaggcat tatatttacg tgtggtaatg
     8821 gagcttgata ggcttttaca atggatggac caaaagcaga acctgggaat agtttaatga
     8881 tatctacgcc agcagtaagt gcctccttta tctcagtaat tgtcatacaa cctggtaaat
     8941 aaggaatttg atataaatta caaatctcag ctgtttgtcg atcaaaagat ggactaacga
     9001 tatattctgc cccagccata attgcgatac gagcagtaaa cgcatctaaa accgtaccgg
     9061 ctccgattat tacatctttt tgatcagcat attttttagc aatttctttg attatttctt
     9121 cagcattagg aactgtaaaa gttagttcaa tccctttaat tccacccgca ataattgaat
     9181 ttattgcttt aatggcctca tccttatttt tcccacgtac aaccgcaatg actccagccg
     9241 cttctaactt acttaaaatt tctacttttt tcatatctat ctccttcctt tatggataca
     9301 tactttgtct agctgtacta acttacaaag aataaattta agcttttatt tcaagtatta
     9361 ttttctactt ccgatttcaa aaaaattaaa ataattatac gcattacgat aacaaatatt
     9421 ctccactatc tgactaagat acatttcatc atctggaatt cggcctgctt taacccactc
     9481 gcttagtaat tgacataata ctcgacgaaa atactcatgg cgaggatagc ttagaaaact
     9541 tcgagaatca gtcaacatac ctgtaaaatt cgacaacaaa ctttgttgtg ccatcgtagt
     9601 caactgttga cgcattcctt cagctgtatc attgaaccac caagcacatc ccaattggat
     9661 tttttgaatc ataccccctt ggaagctttg cattgctgtt gctagtggaa gccaatcagt
     9721 tggatttaat gaatataaaa ttgtttttgg taagcaccca ctcttttcag cggtatcgaa
     9781 caatgctaca acattttgtg tcaaactagc ttgatccccc atcgaatcac caccgacatc
     9841 tactccaatt tgttccttta atatacttga attatttctt aaagcattta aatgcatttg
     9901 catcgtccaa ccatatcttg tatactcaga catcaaatgt aattgaatac ctgtaacaaa
     9961 ttgtgcaatt tctgataaat ggaaatccgt ttttcctgct aaagctttta ttaacagatt
    10021 gtcgagtatt ttttcatctg ctatttcata aaaataagta ttcaaaccgt gatctgctaa
    10081 tcgacagcca atttgatgaa aatgatccac tcgttggctc aatgcttgct tcaacccatt
    10141 aaagtctttg attggtatag ccgctgtttg actaagtttt aaaatatagt tcgaaaagcc
    10201 tgatgatttg atactccaga ccttatctgg acggaaagta ggtaatacct tgaaagaatt
    10261 atcctgttta gaaagctcag tatgatacca caagtcatct gtaggatcat ccgttgtaca
    10321 gatggctttc acatccatca tctccaacaa ttctttcggg cgataggaat ccgtttgaat
    10381 tttttcgtta gcacgtcgcc aaatttctgg tgcattcttc tcagaaattg tcaaatcaat
    10441 atcaaagtaa cgttgtaatt ccaaatgaga ccattcaaaa ataggattac ctggcgcctt
    10501 ctccactgtt tttacaaaag ctaaaaattt ttcataatca tcacctggac cactgatcaa
    10561 ctcttcttct ataccattag ctcgcatcaa tcgccattta taatgatctc cagctccttt
    10621 atcatttaac catacctgag tcaaattctt gtattgctta ttttctagca aatatgctgg
    10681 gtctaaatga caatgatagt cgataattgg ttggtgtttt gcaaatttat gaaaaagtct
    10741 tttagctatt tcattctcta ataaaaagtt cttatctaaa aacatattta aacactcctt
    10801 tccttccgtt agtttttaga agagaaaatt tttcctctaa ataaactccc ttatataatt
    10861 atttactcgc ccccttttca agcgcttccc aaataccatt taaataaact gcacctagag
    10921 ctcggtcata taatccataa ccaggtctac cggtttctcc ccaaatcatc cgaccatgat
    10981 ctggacgaat atagccatta aatccattat cgtacattgt ttttaatatc ttatacatat
    11041 ctagagaacc atattctgat ttatgcggcg cttcgtaaaa atcgagtcca ttgtcactta
    11101 aatatttgat attacgaaca tgtgcaaaag gaatccgatc ccgttgacaa aattcagata
    11161 gaatttcata gacatcatta tctggtcgtt ctgcaatact tccagtacat aatgtaatac
    11221 cattagaagg agaatccact acattacaca accaatctaa atcgtcacga tttttaacaa
    11281 tacgaggtaa tccgaaaatg gaatatggtg gatcatctgg gtgaactgcc attttaatcc
    11341 caacttcttc acatgtcggg ataattgctt tgagaaaata tgcaaagttc tctcgtaatt
    11401 tctcttcatc aacttcttta taggcttcta ataattgctt aatttgtgat aaccgatgag
    11461 gttcccaacc cggcaattca aaattattag tagaattttc tacttctcta ataatttcct
    11521 ccggatcagt agggatattt tttgaaatat aggctaacgt agaagaccca tcatctaaac
    11581 gataatccaa atccgatttt acccaatcaa atactggcat aaaattataa cagataacct
    11641 caatcccaaa ttctgccaaa ttgcgaatcg tctgtttata attctcaatg tattgatctc
    11701 gtgttggtaa accaattttg atatcatcat gaacattaac actctcaata actttcaagg
    11761 taagtccatt ggcttcgact tcatctttta aagatttaat tcgttctctc ggccaaacct
    11821 ctccggctgg tatatccatt aatgttccaa caattccttt cattgctggg atctgacgaa
    11881 tttcttgtag tgagattttt tcttcatttg ccccatacca tctaaaagtc atttccatag
    11941 ttactttcct ccaacttctg ttatatattt attgattgtt ttacgaactg cttgaggacc
    12001 tactagcatt tcgcaaaaga ttttttcagt tttctttcct accccaactt gatataaatc
    12061 taaaccaaag atttctttat tttctaaaat ttcctgtacc gcacaatgaa tctttgaagg
    12121 atcttgttca cctaatttca atactgataa tttatcctgc aactctacca ataacggatc
    12181 aggacttggc acaaaatttt ccccttgatc atcgactgcc agtaaataac gtaaccaagc
    12241 agcaatagcg ataggaataa atgttagatt ttctgcattt ccgtcttttt cgacatactt
    12301 tttaattgtt tcaccatagc gaatcgctac tttttgagaa gtatctgttg caattcgttg
    12361 tggtgtatct ggaatatagg gattaggcaa acgtttatta attacttcat caataaattg
    12421 ttttggatca ataatgcctg gattttcaac tactggtaaa ccttctaaat aacctattcc
    12481 tttgattaaa ccaaccaaat catcatcctg catctctgct gaaattgatt gatatcctag
    12541 cagacaacct gttactgcca aagcagtatg aagtggattc aagcttgtcg taaccttcat
    12601 gatgtctgct ttatcaaccg tctctctatc tgtcataatc acaccagcct tttccaattt
    12661 aggccggcca tttggaaaac tatcttcaat aactaaataa tgaactacct cagtattagt
    12721 gaaagcagca atattggtat gtttttttgt gtgaatgata tctaaatcct cgagccccat
    12781 catcaataga tcttccttta cattctctga aggatttggc gtaattcgat caatcatact
    12841 ccaaggaaaa gtcacttttt ctttatcctt taaataagca ataaattttt cagaaacata
    12901 tccttctttt tgccaagctt ctgcaatcgt aaaaatacta tcttgaaaac gttgaccatt
    12961 ctgtgagaaa ttatcagtag aaaccatcgc aatcggcaac tctccagcat taaatctgct
    13021 atataacaag gatgttacaa tactcatcgt atgttgagga ttgctaggtc cagatttgat
    13081 atcagctttg actaccgaaa agaattgccc tttagaatct tttaaactgt aacctttttc
    13141 agtaatagta aacgttgtaa actgtaattt aggatcttca aaatatttaa ccactctttg
    13201 atagtccgct atattcttcg gattgcaata taaactatca gccgttactg ccaaaagtct
    13261 ttttttgagt tcaccatctc cactcataac aatttccaaa atgtcattgt tatagtcatg
    13321 ataagctttt tccactactt cttcatcaaa tgtttcacac acaactactc ctgtttctaa
    13381 ttcattttga tctgctaagc attgagcaat ttctgcatga aatcctcgat aaagatttcc
    13441 tccaccaaaa tgaagccata ctggatgttg ctggccttct tgcttaactt ctttgacatc
    13501 aaaacacggc agtagaatct gcatttcatt gaattcttcg atagaatttt gccaattttt
    13561 aatcgaaacc ataattaacc tcctcaaact gatatgttag ttttgataca ttttatattt
    13621 taaaacccat tgtaaccgtt gtcaatataa aaagggaata aacttgcttt tttctaccat
    13681 atcagaatga ttgtattttt tattcagaaa aataa
//
LOCUS       DAZKOR010000075        13707 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000075 DAZKOR010000000
VERSION     DAZKOR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.076"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..1537
                     /locus_tag="ACG8ZM_002104"
     CDS             110..1537
                     /locus_tag="ACG8ZM_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493044.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1629..1994)
                     /locus_tag="ACG8ZM_002105"
     CDS             complement(1629..1994)
                     /locus_tag="ACG8ZM_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0493045.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1991..2356)
                     /locus_tag="ACG8ZM_002106"
     CDS             complement(1991..2356)
                     /locus_tag="ACG8ZM_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0493046.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2548..2796)
                     /locus_tag="ACG8ZM_002107"
     CDS             complement(2548..2796)
                     /locus_tag="ACG8ZM_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493047.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2795..2923
                     /locus_tag="ACG8ZM_002108"
     CDS             2795..2923
                     /locus_tag="ACG8ZM_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0493048.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2980..4674
                     /locus_tag="ACG8ZM_002109"
     CDS             2980..4674
                     /locus_tag="ACG8ZM_002109"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0493049.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4741..5070
                     /locus_tag="ACG8ZM_002110"
     CDS             4741..5070
                     /locus_tag="ACG8ZM_002110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493050.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFL
                     TWLDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLP
                     RGYEKGV"
     gene            5448..6581
                     /locus_tag="ACG8ZM_002111"
     CDS             5448..6581
                     /locus_tag="ACG8ZM_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0493051.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6612..6932
                     /locus_tag="ACG8ZM_002112"
     CDS             6612..6932
                     /locus_tag="ACG8ZM_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0493052.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6961..7425)
                     /locus_tag="ACG8ZM_002113"
     CDS             complement(6961..7425)
                     /locus_tag="ACG8ZM_002113"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0493053.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7556..8809)
                     /locus_tag="ACG8ZM_002114"
     CDS             complement(7556..8809)
                     /locus_tag="ACG8ZM_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0493054.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8838..9155)
                     /locus_tag="ACG8ZM_002115"
     CDS             complement(8838..9155)
                     /locus_tag="ACG8ZM_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0493055.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9181..9501)
                     /locus_tag="ACG8ZM_002116"
     CDS             complement(9181..9501)
                     /locus_tag="ACG8ZM_002116"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0493056.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9965..11380)
                     /locus_tag="ACG8ZM_002117"
     CDS             complement(9965..11380)
                     /locus_tag="ACG8ZM_002117"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0493057.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11397..12713)
                     /locus_tag="ACG8ZM_002118"
     CDS             complement(11397..12713)
                     /locus_tag="ACG8ZM_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0493058.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12863..13561)
                     /locus_tag="ACG8ZM_002119"
     CDS             complement(12863..13561)
                     /locus_tag="ACG8ZM_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0493059.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4352 a         2783 c         2359 g         4213 t
ORIGIN      
        1 tttaacggca tttatatgct ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt
       61 cataatattt cccatattgt gcaaaaaaat acaaggcgga ggctctacta tgattgaaga
      121 atatattgag aaaaatattt taagacagct gtttttatgt gggcaattct atgtaaataa
      181 agaagtcaat ctagaaaagc tctccaatct cctgcatgtt tgcaaaacca ctttactcaa
      241 tgatatcaat aacataaaaa aagaatttga agaacagatc gcttatacac atcgggaaaa
      301 ggaccgttac actttatatt tttcagaaca tatacctcga tgcaagatca tgcagcaatt
      361 gtctcaaaat tcgttgtttt taaaaacttg tctgctttat ttggaagaag atgaacctga
      421 ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa
      481 acaagtatta gcttatttta atgactgcgg gatcgaaatc gaccgctact cccctcgatt
      541 cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg tatcgtttag gcggttttaa
      601 ttcttgggag ctacctgaat cgttttttga acgtgccgat cgtttcattg aatccgtaac
      661 ggaaaatagt ggcagatttt atgataaaga aaataaagaa atcttatcga ttggttttgc
      721 tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga
      781 aatcaaaaaa cgtcctattt acaattatgt cgaatcagca tgggaaaaca ctgatttcca
      841 aacctattat aaaaaagaag aattcgcctt tattttgaca ctattcaatc tttgtaatta
      901 tggtttccat tcttatcagt tgattgcgga agactttcaa cagctacacc aagtgtttat
      961 tgacaatact cccgaaataa aagaactagt tgctaccttt gaatctcact tcaatcaaga
     1021 attattcgga aaccaaccgt tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga
     1081 taactatcaa ttattcatgc cagaaaaatt ttatctttta aatgaggaac agacaaattt
     1141 atataaagaa gtccagacaa ttttttccag ctggtccagc cagttacctt atgatctacg
     1201 gttgaatcct aattgcatgc gggcttttgt gatcgaattg agcggaattc tccgtctgac
     1261 aaaagaacat ctgactatct atatcgtcac aaattcggat gttcattatc tgatttatcg
     1321 ggaagccttg gaagctgtaa ctactttcga ttttcaagtt gcaccaacaa tttattcaag
     1381 tatatctgat ataaagaagt atgcgcagca gtcctcaaac cgggtattat gtgaacgtac
     1441 attatataca ccagatgcgg tccaatatga aaatattatt cctatttcga tcaatacgat
     1501 cgatcgagct atcatttcag ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc
     1561 acagcctctt atttgttgaa gatgcccatt tctgtaacaa tctcttgata cacgtcaggc
     1621 tcacaacctc acttattttg gacatctacg ggtccgatct ctttatttaa aaaataagaa
     1681 atgatggttc ggataactac caacgccgct agttggaaaa tatcctgcaa ggtaggtttg
     1741 ataatcgatt cgatgatccc agctgaaaca agaatttcca aacttagaag tatatagcct
     1801 cccaacatct ttttaatagc attgttctcg ctgactcggt cgctttgcac ttttgtatgg
     1861 acaaataaat atttcaaaaa atccttgatt gatagacaaa caccccaaac caataaacag
     1921 atagcaaaga tatcaagtcc cagtacaagt ttttcgaaat atggaagaag aaactgcatc
     1981 aactctgtcg tcatcctacc tctcctcttc tttgtttttc tttgtcttct atttcctgat
     2041 tcaagaaata agaaatgact gttcgaatga taataataag tgcaagcttc aaaatatctg
     2101 tccaagtcgg cttgataact gactcgatga tatcagcaac gactagtact tccaagctta
     2161 acaaaatata acttgctagc agttttcttg tatctgcatt acgagtatta cgttctgcat
     2221 agtttttctt gacatgagac caatcaaaaa gtttgaatag aacaagaacg atcccgacaa
     2281 ttaagatacc gatagaacaa atatttaaaa tagcaatgat ccattctaga aattcaatta
     2341 aatttgagcg aaacatatac gctacctact ttcttttaat ttgttcctat tcttttattg
     2401 taaaagaaag ttaccttgtt cgtctattta aaagttgttc taatgataac ggttcgctta
     2461 aatttttctt tttcactctc ccatttttct ccctccatcc ataaatcgtg gaagtatcca
     2521 aaacaacgta gcaagttaaa ataaggctta gtccgtcatc agcttttcaa agaagcactg
     2581 tctcggtgct ttttttgtac ccaaatttct aaaaatagaa ccatacacct cctcaaatct
     2641 tttcttctta cctaccctat ttttatttac aggaaaatca gacacttctt cttttttgtc
     2701 aaaatttcct ttttcttctc gtcctatcat ttggcattct aataaaaacg aactatcctt
     2761 ggatatagaa agagaaaaca aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg
     2821 catcggttta ggtttgcttg cccttggcgc tgcatatcta ggaaaaaaga caggactatt
     2881 cgaagatgac agtcatttat atgatgaatt cgaatcaatc tagcatccta tttagttgaa
     2941 tcactacgga catacttgga agacattgga ggaatcacaa tgagaaaaag taaagcaata
     3001 atgattggtg ccggtattgc caatatggca gcggctgttt atttgattca agaaggcaaa
     3061 tggaaagggg atcagatcac cttttattca ttggatgatc acggttcaaa tgatggtgca
     3121 ccgaccgaca ctgtagtgga tgaatattgg aataaaaacc atccattaga aaaccaaaaa
     3181 ggctatattg ctcgaggcgg tcgcatgctt aattaccgta cctatgtaga tttgatggat
     3241 ctgttaagtc gaataccttc tgttactgag ccagggatga ctgctgaaga agatacgaga
     3301 gattttgatg caaaacaccg aacctttgac aaagcacgtt tgatggaagg tggcaaagga
     3361 atcattgatg gcggaaaact gggattcaat aataaagacc gtattcttct gactaaactg
     3421 attgctatgc ctgattcaga agaagaaaaa ctagacaata tcactatcga agaatatttc
     3481 aaagatgatc cacacttttt tgaaacaaac ttctggttta tgtgggaaac aacatttgct
     3541 ttcagaacac gtagttctgc tcaagaattg cgtcgctata tgcatcaaat gatttatgaa
     3601 ttcactcaaa tcgaacatct agtgggcgtc aatcgaacac gttacaatca atacgaaagt
     3661 attatgcttc ctttgatcga gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc
     3721 cgtgtcactg actgggaatt caaagaaaca ccgatgcaag atgaaatcac tgtgactggt
     3781 ttgaaaatta ccaatacact gactcaagaa gaagaatacg ttgtggtcga tgaagataca
     3841 gcagttattt tcactaatgg gtccattaca gattcagcta ctttaggaga ttacgaaaca
     3901 ccagctgtag aaaatatgga ttatggtgca gcttctagtc tctggaaaaa agccagtgaa
     3961 cgtttctaca acttgggaaa tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg
     4021 gtcagcttta ccctgacgac gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg
     4081 actcaagaac caggaaatgc cttgaattca tttatttcaa ccacaccgat cacgccgtta
     4141 ggccaaaaag atgtcaatat gtctatcgtc gtgcatcatc aaccgcattt tacgtctcag
     4201 aaaccaaatg aaacagttat ttggggttat ttcctttatc caagacgacg tggcgaattt
     4261 gtggataaac agtatattaa aatgaatgga aaagaaatgc tagaagaact gattggacaa
     4321 ctttcaaaag tagatcctgg accagtgaat attcgtgaaa aagaaacaga aattttcgac
     4381 agcgtaataa ataatatccc tgtctatatg ccttatgctt ccgcgttgtt caataatcgt
     4441 gccaaaagcg atcgaccaaa agtcattcct aaacattcga ccaatctggc ctttacagga
     4501 gaatttgtcg agcagcctta tcagatgatc tttactgaac aaagtgctgt acgttctgga
     4561 gaaatcgctg ctttccattt tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt
     4621 tacgacaaag atattcctac tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga
     4681 ggttgtgaaa aagctgccct gcatcaagta ataagaacaa ccaggctaaa acagcttctt
     4741 atgtttttga tttggttgga cttattaccg cagatgcaag cttttgaaca ccgtttacca
     4801 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cacccaagct aaaactgctt
     4861 cttatgtttt tgacttggtt ggacttatta ccgcagatgc aagcttttga acaccattta
     4921 ccaagaggtt atgaaaaagc tgccctgcat caagtaataa gagcagccaa gctaaaacag
     4981 cttcttatgt ttttgacttg gttagactta ttaccgcaga tgcaagcttt tgaacaccgt
     5041 ttaccaagag gttatgaaaa aggcgtttag tttcgagtat gaagaaaaat tttgtgaaaa
     5101 tggttcctca cgtttttgca taaatttgac ttaataccga gaaactgatc ccttctgtca
     5161 taacctcttt ttatttatgt ctgtttcttt ttgaaaacaa ctattttttc aacgtttact
     5221 ctcttgccaa tccaacatca tctaacggat acaactattt caatgattac cattacacta
     5281 aaaaatcgga aacacacctc tccgaacaca aatacataag actatagaat gtttcattta
     5341 agaaaaaaag cgtttcatta tcaaaaaggt aagatctcct ttaaaatgaa attatctgta
     5401 gtactaataa tgcagacaat tttatgaatc tgaaaggagc atctccaatg aaagcagtca
     5461 cttggcaagg aaaacagaaa atggaaatca gaaacgtaga tgatccaaaa atattagaac
     5521 caacagatgc aatcatccgt atcacagcaa cagccatttg cggctccgat ctgcatcttt
     5581 accatcatgg tgaagcagtt ttggaaaaag ggtatgttgt aggacacgaa ccaatgggaa
     5641 tcgttgaaga agtgggatct caagttcaaa cattgaaaaa agggcaacgt gtggttatcc
     5701 cttttaatat tagttgtggt cattgtcatt tctgcctgaa tcacatggaa agccaatgtg
     5761 atgaatcaaa tccagatcag ttatatggcg ggcttttcgg atttggcaag ttgaacggta
     5821 atcattgggg tggtcaagcg gaatatctaa gagttcctta tgcagactct acttcatttg
     5881 ttgtaccaga taatgatctt cctgatgaaa aagttttatt tctatcagat attttgccca
     5941 cagcttattg gagcgttgaa aacgcaggag taaaaaaagg cgatacagtt gtgattctag
     6001 gttctggtcc agtcggtcta ttcgctcaaa aattcgctgt tatggctgga gcagagcgcg
     6061 taatcgcagt agatccagta cagcaccgtc tagataaagc ggctaattac aatggggttg
     6121 aaacttattt cttagaagac acggcacaag ctggaacaga attgtatgaa ctggctaaag
     6181 gtggcgcaga tgtcattatc gattgtgtag gaatggacgg attagagcct gtcaaagaaa
     6241 aagcgaaaaa tttagtaagc ttacaaagcg gcacaatctc tccattacag atggcatcgc
     6301 aagctgtcaa aaaatttgga acagtccaag taactggtgt ttatatgact ccagcttctt
     6361 cttacccgct gcaagaattt tttatgcggg atatcgctgt caagcatgga caggcaccaa
     6421 ttatccatct aatgccaaaa atttacgcaa tgattgcaga aggactattc gatcctacac
     6481 aaatcattac tcattcgctg ccactatcag aagcagcaca agcatatgag atctttgaca
     6541 aaaaagaaga taaaaatata aaagttgttt tgaaaccttg aattcagtaa aaaatacagg
     6601 tgattttctt tatgcccaaa atcaaacgaa atacccatga gatcgaaata ggaaaagaag
     6661 aagatatcga aatacgagga cgtcgcttcc gcttctattt ccaaaatcgt tacacattga
     6721 tctcactggc tgtcgattta ttaaccggga ttttctatat ttttggaagc cttgcttcct
     6781 taactaaaat ccccgatcga tatggcacct atttttattt ggctggtgct attttcttga
     6841 cgattcgtcc tattctcaga atcgtaagga acatttttat ttacgacgaa aaaaaagcaa
     6901 tgaagagaac aagcaaaaaa aaagaggcat gagaattttt caaatctcat gcctcttttt
     6961 tcaccctatc gtatcacttt gatacagacg agcggtcgtc ccactttctt ccttgaccat
     7021 tgtcaaaaat gtccactcgt atcgttcata aaacgtggta tgatctgtaa ttaagtaaag
     7081 tttttcgtac ccaagttgat gcatttcttt tctgatttcc gctaatatcc gcttggctat
     7141 cccctgctta cgcttttccg gttcaacaaa cagggcacaa agatttggtg tcagatcagg
     7201 gcggttatgg aagtcgtttt caatgacgcc cgctcctccg acgatggttt ctttttcatc
     7261 taagaccaca taccattgag gaatactttc cttattagcg attccagtac gaatactttc
     7321 ctcatacaca acagcaggaa ctccccattt tgatgaaaac caattcgcgc tttgttttac
     7381 taattctggg tgttctctta atttgatcca gttatatgta accaatgttt aaacctccga
     7441 ctaaaataaa acgtttacaa aaattataca agtctttatg aaaattagca atgacaacaa
     7501 tagtgatgta aaaaaaaaac ggaaaacaat cacttgttct ccgttttttg attaatcaag
     7561 catctaaagc ttcttttctt ctttcttgga tcgtagctac cttgacgaac ggtagataga
     7621 tcaaaataga aagaaccaaa ttgacagcgg ctagtacacc acctgcaata ctttttgttg
     7681 ccaatacacc gccaataatt ggtggagtta cccatggagg cataacggta gctgctggca
     7741 ctaaacctgt agaagtcgca aagtaagcaa cactaactaa gaccattggt gtcaaaataa
     7801 atggaatgaa catgatcggg ttcaacacga taggtaaccc aaacatcgtt ggttcattaa
     7861 tattgaagac acctggcgca atcgacaagt ttgttacaac catatatggt ttgtttttac
     7921 gtcctactaa ataaatagct aacagtaaac ctaaagttgc acctgttcct cctaagttga
     7981 caaagctgtc aaagaaaggt ttgttcacga taaacggtgc gctatgccca gctgcgattg
     8041 cattaacgtt tgcttcaatg gctggcgcat tgattgtttg catcaaagga tcgaccatat
     8101 tcgctccgtg taaaccaaag aaccataaga atggtgtgat aaatgcaagc agtaaggcag
     8161 atggataaga gtttgcaagt cccataaatg gttcttggac cactgtatag aaagaaccta
     8221 cgatatcatc aacaccgaat gcagcaaaaa ttgcagcaac taaagcaaac gcagagatag
     8281 tgatcattgc tggtagtaat gaagcaaatg cttttgcaac tgccggtggc acaccatcag
     8341 gcattttgat gatcaatttt tcatttccgc ttaaacgtac aaagatttct gttgaaataa
     8401 tcccaacaat aatagcaata aacaaaccgt ttgagctcat acctaatgct ccgcctaaag
     8461 cgaagaaaga gcctaaagaa atcacacctg cggaaacacc gtcttttcgg tagccgttag
     8521 ctaaatgata agcaagcaag aaagcaatca agacagaaag aatggcaaat gtaccgttcc
     8581 aagcagcagc cccgaatcct ttccatgatt caccaccaaa aattgagttc atcaattctt
     8641 ggaacccggg aatcggtaag ttattgatca ttactgctaa tgctcctaag atcattaacg
     8701 gcatactcac cataaatgag tcccgaatcg caactaaatg ccgttgtgca ccaatttttg
     8761 atgctacagg tataaaatgt ttctccataa atgcaacaaa tttatccata aatcttccct
     8821 cttttcttta ctgtagatta cgcgttcaat cgacgataca gatcaatgat ctcttttgct
     8881 aaatctgtaa aagctataga tgtcatcaga tgatcttgac tgtggaccgt caaaagcgtg
     8941 acttcaacgt gatttccttg tgcttcttgt gtcagcatct ctgtttgaga gtgatgtgct
     9001 tggactaaag attcctctgc atctttgatt ttttcatctg ctaaagtgaa atcacctttt
     9061 ttcgcagcag caatcgcttc cattgcatcg ctttttgcat ttccgccaaa catgatcaga
     9121 cccatgatgg cttctagatt tttttgatct tccatttatt gtatcctccg ataattttta
     9181 ttatttgttt attagttttt ctgcttggtc gagtactttt tcaccattca tcatgccata
     9241 gtcagccata ttgattacat ctaaaggaat gcctttaggt gctagctttt cagcaaattg
     9301 attttgcata aagcgtactt gcggtcctaa aagtaaaacg tctacatttt tagattctag
     9361 ttgattatcc gcatctgaag cagatacagc aaagatgtct gcttccattc cttttgcttc
     9421 tgctgccttt tgcatttttg tgactaggag acttgtactc attccagctg aacatacaag
     9481 catgattgtt ttcttttcca ttttaaacac gttcctttct atacttacac ttgtatataa
     9541 gcaagtagcg tgccaacttt aaaagcgctt taaaaagttg atatcatcac atttaaggtc
     9601 tataccaagt tgcatacaca ctaaaaaaga aagcgcacac taaacacaca aattaatttt
     9661 tattaccttt tttcttttaa cgtgcacaaa aaaagttgtg acacaaaact tgtcacaact
     9721 tttttcaaat aaacggtatt caaaagcttg catctgcggt aataagtcca accaagttaa
     9781 aaacataaga tgttgttttt actttgctat tcttattact tgatgcagga cagctttttc
     9841 acaacctctc aataaacggt gttcaatcag ctgaccctgg gtattagctc aaaacgctgg
     9901 attcacaacc tcttttattt caaataacca gtaaatacaa tgaacatcta caaaaaactt
     9961 ctatttattt atagatttcg tcaggcaaat ccaattctct cttatcaatc aaagtctgat
    10021 accacgaagc agattttttc aatgttctgc ttcgttcatc gttcaattcg attcgaacta
    10081 atccatatcg attcttaaat gcattcattg gtgacacaca gtcagtgaat gcccataaca
    10141 tatagccttc gcaatttgcg ccttcttctg ttgctttgat caagtaatgc aaatgctgtc
    10201 cgataaattc gatacggtaa tcatcttgca cgattccttt ctcatccata tactgttctt
    10261 catattctct tcccataccg ttttctgtta tcagccaagg gatattatga taattttctt
    10321 ttagatacat ggctatatca tacatgattt tcggatagat ttcccatccc ctggaattat
    10381 tcattttctt cccaggtaaa tcaaagactt cataatatcg ttcaggatgg aaaggtgtct
    10441 cttcattcca ttgataacga ggggcgcgaa cgcgtttagg ataatattga ttgattccaa
    10501 ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc tgttgcagga tcgaaataaa
    10561 tcgattgttt ttgacacaac tccaggaact cttctgtgta gtatcctttt accatcggat
    10621 caaaaaatac tcgattataa aacaaatcat acatataagc agctttttgg tcttctggag
    10681 aattggatcg agcatagacc atttcaggat ttaaaataca gccaatcctt ccaccataat
    10741 ttccgctgcg gtaaatctct actgctttag ccgttgctaa aactttatga taattccaca
    10801 tcatccactt ttttgtgttt tgttcatgcg gccagcgaac agcatccaaa tagcaacgcg
    10861 tttggggaac gattggttcg ttgaatgtaa accagttttt taccctatca ccgtaacgat
    10921 caaaggcaat ttgagcatac ttagcataaa aatcaactac ttttcttgaa gaccagccat
    10981 catatttttc caaaagaaaa gctgggactt cataatgttc caaacaaatc atcggttcta
    11041 cgtctgcttc gatcaaagag tcgatcattt cacttatatg ctgagcatat tcttcatcta
    11101 ctatacagtt ctcataatca gtaaaaaaac gagcccaatt gatagaagtc cgataatgtg
    11161 tcagatggat ggatttcatc agatccacat cctgctggta ttgttccata aaattagttg
    11221 ctacggctgg gccataccca ttatgccaaa caaaatgttc atttttatac cagctatcta
    11281 taaacgaatc ctgtccttct ttttttcctt tccaaccttc cgtttgccaa gcagaagatg
    11341 cagcaccaag aataaaattt tttggaatag taattttcat gatttcctcc tgttttttag
    11401 atcccatcat tttccacacg atttgctact tttacaaatg gcagatagat caaaatagaa
    11461 acaataatac aaacaagttg cgtaataact gcaccgatat ttcctgctgt tgccagataa
    11521 gcattgacga tcggcggcat tggccaaggg accattacaa ctgctttcgc cgcaaatcct
    11581 aaagcggttg caaaataacc aatcgttcca gtgataagag gcgttaagat aaacggaata
    11641 gctaaaattg gattcagcat gatcggcata ccaaaaataa ctggttcatt gatgttgaag
    11701 atcccaggag caagggataa cttggtgatt tgtttcatat cttcccgttt tccaactaac
    11761 aatactgcaa tcagtaaacc gatcgtgact ccagacccgc ccatactcat atacatatcc
    11821 caaaatggca tagtaataat gttcggtact tcttttccag cttcaaaagc ttccgtattt
    11881 acagcaattg ccgctaaaag caacggttcc cgtatcggct tcaccatttg atttccatgg
    11941 ataccaatca cccaaaagac ttgcgcaata aacatcaata gcaaaatacc cggtaaccct
    12001 tgtacgacac cttctaatgg gcgttgtacg acattgtaaa tgatatcata aaggtacatt
    12061 cctgtaagtt ctttgatgat aaatcccgca gccgcgatac ccgatacagt cagaatcgtc
    12121 gggaataaag cagaaaaact agcagaaaca ttcgttggca cggtatcagg cattctcagc
    12181 tgtagtttct tctgtttcct caaccagcaa taaagttcaa tagaaaggat ggagaccaac
    12241 attcccaaaa ataacccttt cgtatctgta tattgtttag ccaatacgtt ttctactata
    12301 acagtctgat tttcattcac taattcataa acagttggat taacagataa ataagacatc
    12361 actgcgatga taccagggaa tacaccatcc actttattca gcttggctaa ttcgatccct
    12421 atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga catagctgat ggcagatgcg
    12481 ataggcttta attcttctaa aaaacgcaag aaacttatct gtgctaagcc attttccgag
    12541 tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg taataattac cggaatcaat
    12601 gcagcaaatg cgttttttat caccatgata taccttaaat tattgattct cgttgccacg
    12661 ttccctaaaa cattggctaa tttatccata aaactcattc cagatcccct cattcctgct
    12721 tcttctcctt gacgtcatct ctttttgtct attagtcagc gcttacattt agatgaacag
    12781 aaactattta ctcctctttt ttaccaaata taaaacaaaa attcatctga tgtatttatc
    12841 ataaaataca tcagatgaac tgtcaattag tttctttttc tcctattatt ttctgtaatt
    12901 ctctccgatt tattgccata tggacagtca ttgcatcaaa cgcagcaagt gcattccgcc
    12961 tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt ctcgattttc agatgctgga
    13021 tatcataatt tttattaaac aaagcaattg attcattaat gattgggata atatgcagca
    13081 aggctacgtt tccgctagct tctgcaatca tcgtatgaaa atcgatgtct aattgaacat
    13141 gttcttgtgt gccttctcga aaagaagttt caatcttttg cgttaatttt ttcatttgga
    13201 cgatttgatt cttcgttgca tattccgctg ccaaacctgc catcttaggc tccaataaat
    13261 atctcagttc aaataagtct cgtatcatcc gatcctgatc ttcaatcaaa gaaaaaccga
    13321 gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt ccccgctcct tgccgaactt
    13381 caaggatatt cctggaagct agagcccgca cagcttctcg caccgtgctt cgccccacct
    13441 ctaaataatc ggacaattca tattcattcg gcagtttatc tcctacttgc aactgcattt
    13501 cctttaaata tctaaggatc ttgtcagtag ttacttctac tagggtttta ttttcttcca
    13561 aaccacatac ctccttttca tctgatgtat tttttcgtaa ttattataac acgtttgaat
    13621 catctgatga ttttttgtat acaaaattga ttaatccgtt catagacata caaacgaaga
    13681 agatatttac cataaaaaag actagga
//
LOCUS       DAZKOR010000076        13606 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000076 DAZKOR010000000
VERSION     DAZKOR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.077"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(222..920)
                     /locus_tag="ACG8ZM_002120"
                     /pseudo
     CDS             complement(222..920)
                     /locus_tag="ACG8ZM_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            999..1175
                     /locus_tag="ACG8ZM_002121"
                     /pseudo
     CDS             999..1175
                     /locus_tag="ACG8ZM_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1351..1920)
                     /locus_tag="ACG8ZM_002122"
     CDS             complement(1351..1920)
                     /locus_tag="ACG8ZM_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0493060.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1994..2317)
                     /locus_tag="ACG8ZM_002123"
     CDS             complement(1994..2317)
                     /locus_tag="ACG8ZM_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493061.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2471..3283)
                     /locus_tag="ACG8ZM_002124"
     CDS             complement(2471..3283)
                     /locus_tag="ACG8ZM_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493062.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3610..4485
                     /locus_tag="ACG8ZM_002125"
     CDS             3610..4485
                     /locus_tag="ACG8ZM_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0493063.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4499..4822
                     /locus_tag="ACG8ZM_002126"
     CDS             4499..4822
                     /locus_tag="ACG8ZM_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493064.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4819..5571
                     /locus_tag="ACG8ZM_002127"
     CDS             4819..5571
                     /locus_tag="ACG8ZM_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0493065.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5561..7732
                     /locus_tag="ACG8ZM_002128"
                     /pseudo
     CDS             5561..7732
                     /locus_tag="ACG8ZM_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7844..10015)
                     /gene="copB"
                     /locus_tag="ACG8ZM_002129"
     CDS             complement(7844..10015)
                     /gene="copB"
                     /locus_tag="ACG8ZM_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0493066.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10035..12221)
                     /locus_tag="ACG8ZM_002130"
     CDS             complement(10035..12221)
                     /locus_tag="ACG8ZM_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0493067.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12221..12430)
                     /gene="copZ"
                     /locus_tag="ACG8ZM_002131"
     CDS             complement(12221..12430)
                     /gene="copZ"
                     /locus_tag="ACG8ZM_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0493068.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12443..12883)
                     /locus_tag="ACG8ZM_002132"
     CDS             complement(12443..12883)
                     /locus_tag="ACG8ZM_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0493069.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12957..13496)
                     /locus_tag="ACG8ZM_002133"
     CDS             complement(12957..13496)
                     /locus_tag="ACG8ZM_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0493070.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3831 a         2840 c         2415 g         4520 t
ORIGIN      
        1 aaatataagc tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac
       61 atgtttatag aatctaaata ataaattttt atgtaccgcc cctcaaagtg aactgaaccc
      121 caaaagttga actatttaat ggactgtttc cgatattcta ctggagatag tccattaaat
      181 agttcaactt ttggggttca gttcaaagag ggggggattt tttatcgttg tggaatattt
      241 aaataccaac gcttgtcatg aaaatcttgt gctccaccct tagtatttcc ttctggatcg
      301 tttgtcgcac gcatcatgac gtatactttc ttattaggga aatcacgcat attgaaagat
      361 acatgatagc caacatttcc taaagtatta taagcttgat ttacgtctgg tctataaatc
      421 ccatcagctc ttactctagc tagttctttt ccagtattgt agtccataat gaaaatatac
      481 tcgtatttat aattagcaat gtgccatcca gctacatgca agtttgcgtt ttcgatttct
      541 ccgaactgat caatgtgggc gtaatttgtt ccatctgtca gcgtagaatt tgcagcacct
      601 gctcgagttg gatcaattac tggtttatcc tccgaagtag ttggattatc atccgtaaaa
      661 ccatgagcca aatcataggc tagtttttct ttacttacac ccatttcaga aagataaccg
      721 taaggatctg tatgatcgcc ccaaatattt tgtgttaccc ataaatgcga tttgattcct
      781 ggttggttat aaggagtgtc taatgttaat ggaataccat atttcattgc tgaatctctt
      841 gccaattcaa cgtatacttt atagttcttt tcaaaagttt ctttatcatg tgtatgttgt
      901 aactcgattt gcacaggact tttggctcac gaggttgtcc tactctacat cttgctgata
      961 gatatgctga aaggaaaaca ccaacattat gttttgcttt ttcagctaga acgagacaag
     1021 caggaatacc tttagaagat atcaaaggtt ttttaggtca taaagatgtt tccactacac
     1081 aagtctatgc ccatatttct cccgaagtga aaaaaagatc aatgaatcaa ctagaaaact
     1141 atatagaaga gcaaataaaa aagcactcaa attgagtgct tttttaagct cactatcact
     1201 aaaactatca ctaattttat ttataagcga acatgtttct atcactgatt ttttaaatct
     1261 agcgaaaaac ccttatctta aaggcgttcg ttcaattctt tggcaaggtc ttcaaagcct
     1321 ggcttgccta gaagtgcgaa catggttttc ttatagctca tgtagaaaaa ttttcttgat
     1381 gatgatttta tccatcaatt gagattgact gttttgctct cctatgattt cagctatgat
     1441 tgtccgattt tgcggttttc ttcttaactg gtagttgatt ttcccagttt ccatcaactc
     1501 gctaagcata tccacgactc cgccaaatcc ggtctttttc gtaataaaac ctagtgctac
     1561 cattcgttca tctagatttt ttgctgtaat cgtcacacca gtaatgagcg agtgctgtaa
     1621 tgtttgggcc agctcttcca tcccttcagc attggatacg acggaagctg ttttgttaat
     1681 aggttcttcg tttatttctt ttggttcttt cctctccact ttcactgttt gacgaaggac
     1741 atctagttct tgcaagagcg tttcgtattg ttcttgggtc agcatcacgc cagctacttt
     1801 ttcacgatta aatatatata cgccagttcc cgcgtctcgc gcttgagcaa atacatccat
     1861 cggcgatcgt ttcacttctg tgatcgaaga tgtgggaact tctaatttct ttaattccaa
     1921 tggatttcct cctttactaa tcgcttttta ttatagcaca gactgacttg ttttgatctt
     1981 ttttccaaag gttttatcgg tttttgccta accagtaatt gatatcggta agaagtcttt
     2041 cgataccaaa tgaaggatgg tacagatacg ttttttgatc ttcgttatgg tctagtggaa
     2101 aaaaatgatc gatgatcact agatctgcat accgaggcgg cttatttcga gtacatacat
     2161 gtatagagat ccgttttcga tgtcttagcg ttttgttcag taccccagca agtcttcttg
     2221 cttcttggtg ttcatagcga aaaaaaaaga cgattttcac ttcaggaggg tggacatcta
     2281 aataccctcg ttcctggagt gttttcagta aaagcatccc ccactctact gcggggtgtg
     2341 caagtgcttg ttggatcgct agccgaaatt gatatagttt atctttggaa ttgggaaatt
     2401 tttctaatgt ttcctgataa taacgaactt cttctatcag ccggtcatgg ataccaatta
     2461 cgtaacgaag tcattcttgg taaagtactt ttgtcagctt tgatttgctt tcgctctcaa
     2521 attcgctatt cagccaaaac agcgaatcaa gcttttcagt cacaatcgtc aatttccgat
     2581 agtcttgtgt ctcttctatc cgtttccacc gcttttcagt aatctggtta cagctcaagc
     2641 gcaaaattgc tagataattt ttttctttta ttggcaaaat ctgctcctct ctaaatagca
     2701 attcaggaaa caattcaaag aatagtgttc gactgtctcc taaaatctcg atttctgccg
     2761 gatcctttct ttgcttcaat aattggatga acatctcaga aaaggactgc tctccatcat
     2821 ctgcaaaaaa atgcggaaaa tctttcaaaa agcaaaacag cagatatctt acttttcttt
     2881 ctgaaccaaa gatctgtgct tttttataag aaaaagaaag gtggtactcg ccaaaccact
     2941 ttctcaatcg ctcttgcgta gaacgatcgt acttctgctc gtaaggttca ttcaatagca
     3001 gatttttcag taaacaaaac gggggtgatt ggatcagaaa aacaaacaag agataaggtt
     3061 cgctatatcc ttgatcataa actaactgaa cggttttctt atgaagaatc agctgaatgg
     3121 gacagtcgct ttccgccaaa cgtttcagat gccgatccac tgttttaaca tcgattttca
     3181 gttcttcagc taaatcagct tttcgccaga tcaaatcttt cattagattg attagtgaaa
     3241 ataaattctg tgcatttttt gataatagtt tttgccatct catcgatcat ttctcacctg
     3301 atttctaaac atattgtttg atcaaaatgg gttcactcct aaaaagataa aaaactccct
     3361 ttctatttat ttaaatacgc aaatcttcct cattattttt tattttactc aatttaatcg
     3421 tattctcatt tcaaaacgaa tgtactttaa aaaaacataa taagatcact ttgcttgtag
     3481 gatgtattcc cttccattac aatgaaataa atagcgattt tatgagaagg tgatgtgaaa
     3541 atggaagaaa aaatagaaga atccaaacaa aaaatcgatc agttgatata agaattagaa
     3601 aaagcggtca tcggcaaacg ttcaactatc caattgatga tcgtggccct attagcaggt
     3661 ggtcatgtct tattcgaaga tattcccggt gtaggtaaaa cgttactggt caaagcacta
     3721 gcaaaagctg tacagggaac cttcagcaga gtccaatgta ctcctgatct gctgcctagt
     3781 gatattcttg gtttttccgt atacaattca caatcaaaag agtttgaatt tcgtccggga
     3841 ccgatcttta ccacgatctt gttagcagat gagatcaatc gtacgactcc tcgtactcag
     3901 tcagcacttt tagaagcaat ggccgagaat catgcaacga tcgacaacaa tacataccct
     3961 ctagacaatc atttttttgt cttagctacg caaaatccaa tcgagtacga aggaacatat
     4021 cctttgccgg aagcacagct cgatcgtttt ttattccgac tacaaatcgg ttatccttct
     4081 tttgatgacg aacttttgct acttttggat aaattcgaaa aagtgttgga taacttaaat
     4141 gaagtattga acagttatga aatagaagaa ataaaaacaa atgtggcgaa tgtctttgtc
     4201 agtcccgaag tggcatctta tgccttgcag ttagtgtcag caacaagaag tcatgctgcc
     4261 atccagcttg gcatcagtcc acgaggaagc ctttccttta ttcaggcagc aaaagcttat
     4321 gctttaatcc atgggagaaa ttacgtcacg cccaaagatt tgcaggattt agttccttac
     4381 gtgttttccc atcgcctgat tttttacgat cgttcattga aggaagacga aaagaaacaa
     4441 atcattcata ctcttgttgc acaaattcct attcctgtga ggtagattga ggtagacaat
     4501 ggaaaaaatc aaagattatt tcattaattt tgtgttattt ctggtatatt tcatcattgt
     4561 gctttttgct cttgtattca acaattcttt aggctggttc ttgttggcct tttttactct
     4621 ttatgtatca tggagcttga tttcattaag cagctttcta aagaaacttt cggttacttg
     4681 tacattgcag ccactcgttt atcgcaaaaa aagtatgcag gttatttttt ccatagcaaa
     4741 aaagagaact ttttctttac ctctccctcg tttacaaatc ggcttcccag caatctttgc
     4801 agaaaaaaac aagagatttt gattcttaca aataaaccgc aagaagtcca attgctttgg
     4861 cagccaaaag aaagaggatg ccatgaaaca ttaactgtca tttttacagc ctatgatagt
     4921 ctaggacttt tcagaaaaag tgcaaaaaga gagatctctt ttcctgtgac tgttctgcct
     4981 gctttttgta agattcaagc agaaaaatta cagagagttc tggaaaaaaa acagcagctg
     5041 aacgcgtacc aaagaggtct tgattttcgg gaacatcgcg cttatcgtcc aggtgattct
     5101 ttcaaccgaa tcgattggaa attgtctgct catactcaag agtggttata ccgtgaatac
     5161 gattatcaag aagaagaatg ttctcctctt ttttgctttt ggggttcgcc cagtccaaaa
     5221 tttgaacctc tgctagactt gtatttttct ttatggcatt tgagaaaaga aaagcagccg
     5281 gaacttctga tattaggcaa ccgcgccttt catggcaaag accccggtca tacctatttt
     5341 gcttctttgg aagctggcga tgagaaagct tttttcgcac acttgaaaaa atatgcaaaa
     5401 aaacaaatcg tcctctttgt tcctgagaat tctcctgaag tctcagaagc tattgaaaca
     5461 ttgtcaaaag atcgaacggt ttatcttgtt tatttcgtag aaaaacaact catggtccaa
     5521 gaaaaagaca agatatgttc attgccagga ggtgaatgga ttgacgatta aacgggaaaa
     5581 atggctgcgt ggcgtttact cctttgtgat actaagtgta ggtgctccga tatttgtagt
     5641 agctaatcaa ttgcaaaaca gctaccttct tatcctaact attggtgtcc tatgccttat
     5701 tgtctgctgt attcctaaaa aatgggtacg tttgctgctt tctttgccag taatgatagg
     5761 ttgtttgttt ttatattttc cttcgaagac attttccata ttatggatct tacagttttt
     5821 ttcgtcagtc acagaggaat tcttccatct atcccaaggt aaactttatt atttgccaga
     5881 aaagacagct ttttttctca taatgttgct gatctattta tttattacat tgaccgtgga
     5941 ttacgactat tggtacgcac cctttctttc tttgatggct tacttcatga tgcttaccgt
     6001 ttttcggaaa aaagatttac tgtacgaaat gattgcggta gtaactgttt tgttcgtgta
     6061 tctggcagca aggcaatttt tctccatccg gttgttgtcg ggaaacactc gattccagtc
     6121 attgactaca gttctgctta tttgtttctt aacttttagc gctatcttgc ctttatatct
     6181 tccgaaagta caccaatctc tcgaggaaac gagtgaaccg atccgagaat atcttaatga
     6241 tgaagggctt tatgacacac tccatgagta tcaattaacc ggaagtgcaa gaacaggctt
     6301 cagtgagaat gatgaacagc ttggtggacc tttatatgat aatggcgaag ttgtttttac
     6361 agcgaaccaa aaaggaaatg gctattggaa gatcgaaaat aaaaatcatt acaccggttc
     6421 tggatggata gaagatcagg acgcacccat ccaaccaatc caagaattcc ctgttgacat
     6481 cacgagctac caaaccgtta tagaagacaa agaaaacatt acattgaccc taaaaaaaca
     6541 agttttctta ccttatcctt atggaaatac taccttgact gatttttcat tagctagtgg
     6601 acaaacgctt tactatttgc ctgaaagcca gcgattcatt cctgtcccga atgaatcgac
     6661 acgttctgca actttacaaa cagaaaggtt agtttttacg agagaagaat taaaaagcag
     6721 tcagttaaaa gctgatgatt tagaaaatca gcagacaaat cttcaattgc caaaaagttt
     6781 gcctaaacgt attgaggact tggcggtcga attgaccaaa aatgaagcaa ctttaattcg
     6841 taaagtcgaa gctgtctaac agtatttaaa aacaagcgga aatttccgct attccaaaac
     6901 ggataccggg catactccaa aagaacacga ttatgtggat tatttcctct ttgattcaaa
     6961 agtcggctat tgtgacaact tttcaacttc tatggtcgtc cttcttcgaa cacttggtat
     7021 cccagctcgc tggacaaaag gttttacaaa tgggctcaga acaggcacag ataatcaagg
     7081 gaatgaggaa tacagcattc tcaatagtca tgctcattct tggccagagg tatactttcc
     7141 tggatttggc tggcttccat ttgaaccaac cccttcattt gccaatccaa tgggttcaca
     7201 accagttgct gaaacaaccg aaaccgtcca gcctgctatc gaacaaccaa atgaaagcga
     7261 aaatatggca gaaactattt ccagtgagag atctgaggtc attccatcag aagaaacaac
     7321 actcacacca gcttataaaa tcgtcttatt cagccttgga gtgtgtttat ccgtcttgtt
     7381 gttctttcta ttgttattca gaaaatctct gcgctggaca agttatctta tctatcttca
     7441 tttttttgct tctactaata cgattgcata tcaaaaactt cttaaccctt tggaaacatt
     7501 ggtaccgcgc aaacccgcag aatctttgca ggaatatagc ctgcgaatag aacaagaatt
     7561 caagctagat ctgcgtttca ttcgattaac tactcaatat gaagcagaaa tatatggacg
     7621 acaagcagat ccggtaaaat tcgataaaaa aacctattta gaaataacgc atataataag
     7681 aaaaatggta tacaaaaaaa ttctgacaaa agggagccgc ttcaaaaaat aagataaaat
     7741 ttctggagaa tctacttctg gaatgccgag aaccagctga ttgagtatcg attactcgaa
     7801 aaaaaggttg tgacacgttt tttgtcacaa ccttttttca ttattatctg attttcaatg
     7861 tcaaagcatt tagagctaca actaccgtac ttaacgacat caagacagca ccaactgctg
     7921 gacttaatat gataccaatt ggcgcaagaa ttccggctgc tagcggaatt gcaagaatat
     7981 tgtaacctgc cccccaccag aggttctgga tcattttctt tcgcgtttgc ttggctagat
     8041 caagaaaatg caaaatatct tttggatcgc tgtttgtcaa tacgacatcc gcagaatcga
     8101 ttgcgatatc tgtccctgca ccgattgcca tcccgattga agcacgggct aaacttggcg
     8161 catcattgat cccatcaccc accatgatta cttttttccc ttggtcaagg tatttctgga
     8221 caacagcttc tttatcatct ggtaataaac cgccataata ttcttcgatc cctaaataat
     8281 ctgctactgc ttgggcagcg tttttattgt caccagtaag catgacagga gtgatttttc
     8341 tttctttcaa tgttcggata aattcttttg cttctgtttt tactacgtca cctaaagcaa
     8401 cgattgcagc tagatgattt tctaaaatca ggaagctgat ggtatttcct tgttcttggt
     8461 aaggtttcaa taatgcttgt tctacgttta atccgagacg ctcaacttct ttttcattaa
     8521 ctattttcac ttgttgtttt ttgactgttg cctctagccc gactcctgaa agatttttta
     8581 gattttcagc ttgataaggc ttgatttcat gttctttcaa ataattcata atgccaacag
     8641 ccaatggatg attggcattc gcttccaagg ctcctagata ttgaaggatt tcatttttat
     8701 tgaacgcatc atcaagtact tcgacacccg ttacagtaaa ttttccttct gttagtgttc
     8761 ctgttttgtc cagcatgatc acatctagat cattggcttg ctccagtgca ttgcggtttt
     8821 tcaaaagtaa tccgttttta gctgctattg atgtggaacg agcaacaact aatgggatag
     8881 ctaaacctag agcatgagga caggcgatga taaagactgt taccatacgt tctaatgcat
     8941 taggaagatc tgtcaagaac aaccaggcga tgaatgccag gatacccaca attaatgcga
     9001 cataaaacag ccattttgcc actttgtcag ataatgactc tagcttggat ttttctcctt
     9061 gtgcttttcg caccatttcc ataaccttcg ctagataacc attttcacca gttccagtta
     9121 cttcgatttc gatcgttcca tccccattga tagaaccgcc aatcactttg tcattggctt
     9181 gttttttgat tcctcttgat tcgccagtca cagctgattc gtcaacaatt gtttcacctt
     9241 tcaagattac accatctgtt ggcattttat caccagaccg gacgatcaaa cgatcacctt
     9301 cgtttacttc tttcaatgaa actgtctctt cttttccatc tttcgtcaag cgtttcactg
     9361 attctggcag taattctgct agtttttgta aagcattgct ggcattcgac acagcattca
     9421 tttcaatcca gtgacctagt agcataataa cgatcaaagt agccaattcc cagaagaaat
     9481 ccatgacgtg tgtatgtgga ttgagcagat ttgcgataaa cgaatagaca ctataaatat
     9541 atgcaactgt aatccccatc gcgatcagtg tcatcatagc aggacttttt tgtttcaatt
     9601 ccatcttagc tccacttaaa aatggctgac cgccatagat aaatagtata gttgctaaga
     9661 tcaatacgag ccaatcagaa ccaggaaacg ttacttgaaa tggaaattcc attcccatca
     9721 ttggtgaaaa aagaatgatt gggatagcta ggataagcga tagccaaaat ttttgtttga
     9781 aattccccat gtgcatggaa tgatccatgc cgccgtgttc gtgatccatg tgcatcgaat
     9841 ggtccatttg tttgctgttt gctatatctc catggttatg tcccatgtgc atggaatgat
     9901 ccatctgact atggtcatgt ttcatctcca tctgtttttc atgagattct gtactttctt
     9961 tgtttactac ttctttttta tgctcatgtt ttttctccat atcgttccca ttcattttta
    10021 ctccatccct tctcttactt tttcatgtgt cgatttaagc acagggagtt caacaataca
    10081 ctgacggaac taaacgccat cgctcctcct gcaatgattg gattcaaaaa gcctagtgcg
    10141 gcaaaaggaa taccaatcgt attatagaca aaagcccaga aaagattttg tttgattttt
    10201 ctcaatgtca acctagacaa ctcgattgtt tgtgcaatgg atgccaaact gctattcatc
    10261 agcgtcacat cggctgtttc catcgcaatg tccgttccac ttcccattgc cataccgaca
    10321 tcagctaatg ctaaggccgg tgcatcattg attccatcgc ctgccatacc gacttttttg
    10381 ccatctttct gaagtttttc tacgtaggct gctttatcct ctggaagtac ttcagcaaag
    10441 atatgctttg gatcaatacc aacttgttta ccaatcgttt cggctgccag tttgttgtct
    10501 cctgtcagca taaaaacatc taatccttta ttctggagct gcttgattgc ttgtttgact
    10561 tctagtttga tttgatcagc cacggcaatc aacccgatca cttgcttttc atcagctaaa
    10621 aacatgactg tttttccctg ctgttctaat tctaacgcgt gttcttggta ttcatcaaat
    10681 gataggttca attcaattaa tcgtttacgt gtacccgcaa aatagcgtac tccgttaatc
    10741 gtaccgctga ttccagcacc tgggtgggct gtaaaatcaa tcatatcgta tgttgctgct
    10801 tcttgcagtt ttccgtattc cacgattgct ttacctagtg gatgttcaga agcttgttct
    10861 aaagtataaa aaatagatag gacttcttta gaaccgacta gatctgtaac tttgggcttt
    10921 ccttctgtaa tcgttccagt tttatccaaa acaatgctgt ctaagtgagc agctgcttct
    10981 aatgcttctc cgcctttaat taaaattcca tttcgtgcac caacacccgt tcctaccata
    11041 atagctgttg gagtcgctaa acctaatgca catggacacg caatgactaa tacagagaca
    11101 ctatggatca atgcgagttg ccaatcacca gtgatcaaac ctgtagctat gagcgtgata
    11161 aacgcgatga ctaatacgat cggaacaaaa atccctgaga tccgatctgc gattttttgg
    11221 attggggctt tgctcccttg agcatcttcc accatttgaa tgatttgcgc taatactgtc
    11281 tgcttgccga cttgtgtaac ttccatgtga agtagaccat tcgtattgat tgtcccgcca
    11341 aagagcgtgt catccggatt cttttctaca ggaagatttt caccagtaag catactttca
    11401 tctatcgcac tgcttcctga aataattcga ccatctgctg gaatctgctc accaggacgg
    11461 atgactagtt gatcaccaac gactacttct tcaatcgcta acgtttcttc tttcccgttt
    11521 cttatcactt gggcggtttt tgtttgaaga gacatcaatt gtttgattgc attacctgtt
    11581 ttcgtttttg ctgtatgttc caaatatttg ccgagcaaaa tcagcgtgat gatcatactg
    11641 ctgctttcaa aataaagatc ttgggggtgt ccacggaaaa atccgttgta aatgctcaat
    11701 gcaaaggcag ccgatgttcc aattgctacc aatacatcca tatttggtgc tttcgtcttg
    11761 attgcatggt acgcaccttt ataaaaacga gcaccgacat aaaattggac aggagcagac
    11821 agcactaact ggacgattgg aaaatgaaaa aagtgaacaa tcgccgcatg gcttcctaac
    11881 atcatagcaa tcattgacaa catcaacggc aaagtcaaaa ccgtactcaa tataagatcg
    11941 aatagcattt ttttcaaata cgcttgcttt tcttctgcaa ttttttgttt gtgggcttca
    12001 tcgtacaaaa ttgcaccata gccgatgttt tctacgcttt ggattaattt ttcagtggtc
    12061 gtcccttcat atttgacact tgctttttca gtggccaagt tgactgtggc attgaccaca
    12121 ccaggttgtc cgttcagctc tttttcgatc ctagcagaac aatttgcaca agtcatacct
    12181 gtaatgacga acgtatccat tttggtttct gctgttgcca tcaaatcacc tctgctttat
    12241 aacccaatcc attgattgct tgacaaatct tttcggattg aacttttgtt tcatcaaact
    12301 tgaccgtacc gtttgctttt ttcaaattta ctttcacttt ttgcacgccg tcaagtgcgc
    12361 ttactgtttc ttctactctt gctgcacaat gattgcagct cataccttcg attgaaaatt
    12421 gttgtttcat tttttcttcc tcctattgat gttgtttaca ttcacattga cctgggatac
    12481 agttacattc gatcgtatct actggttgct tttgttccag ttgtttcaaa atctcattga
    12541 tatcttcttg agtcaaagtg gcttcttcta ctagatccgc aatcgtctct ccaatccttt
    12601 ttgcacagat atgggaaaaa agattttccg tcgcactgcg aactgtttcc atctccgaga
    12661 cagctggata ataaatgaat ttttttcctt cttgttctgt ccagatcact tcttttttta
    12721 ccaaacgacc cagcaaagtt ttgatcgttg ctggtttcca atccatcgaa tcagctaaga
    12781 tctgcgtgat ttcttgggct gtcgtctgtc caagtgtcca aataacacgc atgatctccc
    12841 actctgaatc actgatcttt actggcgttt tgactttttc catttatttc acacctttcg
    12901 tttacaaatg taatcattat cttatgaata cagattacac ttgtaaacaa aaactgtcaa
    12961 ttatttttgc tctctatttt caatttagtt aaaaattctt gtttctctcg tcttgtttta
    13021 aggatgatca gtttatttct tgcattagct acttggatca gctgctgtat tttcggttca
    13081 ttgttttctt caaaatgcca gataaaagat aaaaattcta agtattccct gtttagtttc
    13141 tccgaaaatg attcaggcat atctggccga gattttttgt ttatctttct ttttagagaa
    13201 cgccaaacta ctcggtagat ggcgagataa cgcggcactt taagccaaat gatcttatct
    13261 gcttctttaa tcctttcctc taatgtagaa gtataatttc cttcaacgat ccatgacgga
    13321 ttagatacca taaacaattg ctgttcttgc aaaaaccact tttttgcctg aatactgtag
    13381 tctgttgtat gccataaaag gtctagcggc agtagcggaa agtcggtgat ttctttgatc
    13441 ttccttgcga gggtcgattt tcccgaccca ctttgtccaa tcacgatgat cttcattctt
    13501 ctccctcact tctctttgga atttggttcg atcataccac ttataacgcc gcaaaaaaac
    13561 aagggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaataa
//
LOCUS       DAZKOR010000077        13237 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000077 DAZKOR010000000
VERSION     DAZKOR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.078"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(417..1031)
                     /locus_tag="ACG8ZM_002134"
     CDS             complement(417..1031)
                     /locus_tag="ACG8ZM_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0493071.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1637..2014)
                     /locus_tag="ACG8ZM_002135"
     CDS             complement(1637..2014)
                     /locus_tag="ACG8ZM_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493072.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2314..3195)
                     /gene="galU"
                     /locus_tag="ACG8ZM_002136"
     CDS             complement(2314..3195)
                     /gene="galU"
                     /locus_tag="ACG8ZM_002136"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0493073.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3238..4257)
                     /locus_tag="ACG8ZM_002137"
     CDS             complement(3238..4257)
                     /locus_tag="ACG8ZM_002137"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0493074.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4890..5894)
                     /locus_tag="ACG8ZM_002138"
     CDS             complement(4890..5894)
                     /locus_tag="ACG8ZM_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0493075.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5907..6956)
                     /locus_tag="ACG8ZM_002139"
     CDS             complement(5907..6956)
                     /locus_tag="ACG8ZM_002139"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0493076.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6973..7794)
                     /locus_tag="ACG8ZM_002140"
     CDS             complement(6973..7794)
                     /locus_tag="ACG8ZM_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0493077.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7781..8560)
                     /locus_tag="ACG8ZM_002141"
     CDS             complement(7781..8560)
                     /locus_tag="ACG8ZM_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0493078.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8577..9047)
                     /locus_tag="ACG8ZM_002142"
     CDS             complement(8577..9047)
                     /locus_tag="ACG8ZM_002142"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0493079.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(9059..9478)
                     /locus_tag="ACG8ZM_002143"
     CDS             complement(9059..9478)
                     /locus_tag="ACG8ZM_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0493080.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9559..12336)
                     /locus_tag="ACG8ZM_002144"
     CDS             complement(9559..12336)
                     /locus_tag="ACG8ZM_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0493081.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12527..12751
                     /locus_tag="ACG8ZM_002145"
     CDS             12527..12751
                     /locus_tag="ACG8ZM_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493082.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3892 a         2758 c         2151 g         4436 t
ORIGIN      
        1 gaaaaaggta tcctgttgga caattttgtt atatcaggta aacttcattg caataatgat
       61 aacaaatgta ctataattac tttgtaaaga gttttgggga agactctttc atcaacgtgt
      121 attaagagac aagaaagaat ccatatggat tctttcttgt ctctttttcc actacttttt
      181 atttttctga cactacatat tcttataact catttctcat aactgtgaaa caacttataa
      241 aaataacaaa ctttaaacaa aacaagctta aaatcaaaga tgatccatct taaaataact
      301 aaaacaaaaa aagtagtcgt tgttaatcct cattaataat cgttttcttt ttttcaaacg
      361 ctccattttt atgtggaaca ttattttttt aaggaacttt gtcaacatgt ggataactat
      421 tttccacttg tgtcctggac tagctgtttt gctgtaaaat acaaaacttg ctgcagcaaa
      481 aagatcgttg caagagaggc tgttccgcag atcgctggat ctagatttgg caaagtcaag
      541 actgcatcat gtaaattggc gtggttgtcc aaaaccaaat cagcaaattc aaataatcgc
      601 aaaccactgc tatgttgcga tttgatactt cgtgacaaat caaatccagt aacaatgatt
      661 aatggatgac cattttcttt gatccactga gctagttcga tgattgccgg gtttcttcct
      721 tcattcgaaa gaagaagaaa aatgtcttct gatttgacag aaagctgatc aataattgcc
      781 tgtcctgcac cttctagcgt ttcatagaca cctccggctg gattttcaat caatatactt
      841 ggtacaagat ccggggtttg atcccaaaat tcaaatgcta caccacttag tgtgcggcta
      901 gcatatatct gcaatctacc accttgatta atttgtttcg cgatccaagt gccagcttga
      961 agcatcagcg gctcttctat ccttaatttc tcttccaatt ggtgataata gtcaaaataa
     1021 cataaactca tttatctctc ccgcctagct atacttgaca tgaatagaaa cagctgccta
     1081 atctaaggaa gtacataaaa aaacagtagt tagacagtaa cacatgtggt ctttgctgtt
     1141 tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc ttgacatagc aaataaaaca
     1201 acgtgcagac catggaaaat atctacatag gggtagaatt ttcaccctag attaggtaca
     1261 ataaattagg tcgatgcact gcattgacta tggcaaaatg atcggtcagg gtgtatgtgt
     1321 gtgactggtc attccctgat tcttagcgcc aactaagggt cagggagcct ttttttattc
     1381 agctgtttct atctgaattt attgtaagta atctcttatc caagtgcaag caagaaaata
     1441 caaaaaaaca ttggtatgat ttatatatat ataaataaca taaaaaaagg aatggcttat
     1501 cccgatacaa caagcattcc tttaaaaaag attcttttat ataagtgaaa aaagcttttt
     1561 tccaccaatt aaataaatct ctcatctcaa tagtttatcc attattttat ctttttttgc
     1621 gtatacctac taaatattac cgtcttcttc tcgttcttct actttgttcg agagaatctg
     1681 tactagaaaa agtagttgta tgtctacctt ttctgcctat ccgagacttt cttttcatat
     1741 atgggatata aatgattaga tacagtacag caaaaaataa agcaaatacg caagcttccc
     1801 aactacctaa cgccaattca accatcaagg tagcaacccc aacggatgct gcataccaga
     1861 taaaaatcag aaacgtttct ttttttctga ccatgcgaaa catgataaat agactaataa
     1921 gcaatagtgg caacacaaat atccaattgc ttccttccat cagccacttg agtatatcaa
     1981 gcagcgactg tgcaacagcg actaattttc ccatagtgaa cacacacctt tttttataat
     2041 taaaaaattc gcctttttat tctactatga atcttctggc ttggctttca tttctcttat
     2101 gtaatcattt tcttttatac aacttaaaaa aaaattaaac cattagcata atattttctg
     2161 aatctattca cgaaagtctt tttctatctt tcgttttcca cgactcaact gatctagagt
     2221 caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc tgccgcttta ataacaaata
     2281 gcctttcatt ctcagcaggc cacttatttc cagttaactt tctgttctta gctttttact
     2341 caactggata atatatgcac ttaatgaagt cccaatttcc gaatgttgaa gtccatattc
     2401 aatatttgtc ttcatatatc ctaacttatc gcctacgtcg tatctttttc ctttaaactc
     2461 tctcgcatag accgcctctg tctggttaag agtctgaagt gcatccgtta gctgtatctc
     2521 gtttcctgct cctggttctt gagtttctaa tatctcaaat attttaggag tcagtagata
     2581 acgtccgata atggctaaat tacttggtgc ttttcccatt tcaggttttt ctacaaaccc
     2641 ttttactctg caacgaccct tttcgctatc tgttttagga tcaataattc cgtatttaga
     2701 tgtttcattt tctggtacac gcatgacggc aacagcggaa gcttgtgttt cttcataatc
     2761 catcatcaat tgttgagtca atgggatatc atctttcatc aaatcatccc ccagcatgac
     2821 gacaaacggt tcatttccta taaatgcttt tgcttgaagc acggcatccc ccagtcctct
     2881 tggatgagat tgacggatga agtgaagatt gacttgagtc gtttcttcaa ctaaagcgag
     2941 taattctgtt ttttcctttt cccgcaaatt gttttccaat tcgatatttg catcaaagtg
     3001 atcttcaatg ggtctttttt cttttcccgt tacgatcaag atgtcttcga ttcccgaatc
     3061 caatgcttct tccacaataa actggatcgt tggtttatcc acgattggaa gcatttcttt
     3121 tgccatggct tttgttgccg gcaaaaaacg tgttcctagc ccagcggcag gaatgactgc
     3181 ttttttgacc ttcacttgct gttcctccta aacgacgatt aactattgat catctattta
     3241 acgaagagaa aattcattct ccatttttcc gtcacgtaac atgatttctt ttgctgcttt
     3301 tttgatatct tctccattat acaaaacatt atagattgtt tgagtaattg gcatctcaac
     3361 acctagctgt tgagctagct ctactgctgc ttttgtcgtt gccacaccct caacgaccat
     3421 tcccatgttt tctagaactt cttccagctt atgtccctgt cctaacaagt taccggcacg
     3481 ccaattgcga gaatgaacac ttgtacaagt aacgatcaag tcccccacac cactcaaacc
     3541 gataaatgtc agaggattgg cacccatcgc gacaccaaga cggctgattt ctgccaatcc
     3601 tcgagtcatg attgctgctt ttgcatcatc tccgaatcct aatccatgga tagcgccggc
     3661 accaattgca atgatatttt tcagtgctgc accagtttct acgccaatca catcattatt
     3721 tgtataaatc ctgaaataat cattcatgaa cagtttttgt acataggctg ctgctttttg
     3781 atcaatactt gcagctgtaa ttgttgtgat atcatgtact gccacttcct cagcatggct
     3841 aggaccagat aatacaacaa ttgcctgtct tttttcttct ggaatttctt cagccaaaac
     3901 ttctgagatt cgtttatgcg tatcttgttc tagtccttta cttgcatgta taatgattgg
     3961 cttagtattc attttaccaa taagttcctg tgcgactgaa cgaatcgctt ttgttggaac
     4021 aacaaataaa atagcatctg cgttatccac tgcttctgcc aattctttat atccgataat
     4081 cgattctggg atcgtcaaat caggtaaaaa atgctgattt gtatgatact tattgatttc
     4141 atcaatctgt tcaggtacat ttccccaaat acggacatca tgcccgtttt ctgccaatgt
     4201 ttgtgctaaa gcagtgcccc aagatccagg accaagaact gcgatttttt gtttcatgga
     4261 tattcctcca aataaataat tgataatttc cattatagta tacatttctt aaagttggga
     4321 ctaatttcca ttgtttttaa gtgtttgttt acttctttta tcgtttttac ttgaaataat
     4381 caatgatatg ttacaatagt ctacatttta tttttacact cgttatgctt tgcttgttcc
     4441 taagaggaag cagttttgac aatgttacta ccagcattac tattaggagg ttaggaaact
     4501 tctcctagtg agtattgatt taaaatacat aagagacaga ttgaacattt ctaaatctgt
     4561 caggtcctgc aacggacaga ttgtctctgt tgacggttga cataggaact tttgttttag
     4621 tcaacattct ggatacaaaa cgctatgtaa actggcgcaa ctcgttgtac ttggactttt
     4681 actacggatc catgcaacaa ctaaatagta ctattgcata acaaaaaatg gttggcaccc
     4741 acatgtccca ttaaacagat tataagttga actgtagata atgcaaaaaa tgtgttttat
     4801 aacgtaattg atgccaatat tttaaaaaaa taaagggagc agttatcgct gctctcttta
     4861 ttttttatat tctttttatc cagtctatcc taataatcaa actgacgata gtagcggcgt
     4921 atctctaatg gatggcagtt caactgctcc acatgagcat cgatgcgttg tgtcactaca
     4981 tgcatcaata agaaagacaa aacacgtccg cgatattttt cactgatacc ttctactgga
     5041 tagtcttttg tgtctatcaa tgtataattg ctgcaaattt ttggcaaaag tttagccaca
     5101 cgttcagata acactctctg ttcatcttcg cctaaaaata atgtgactgg ggttgtttct
     5161 tctacgatct ctaacgtccc gtgaagaaac tcggcagcat ggatagattt cgatggaagc
     5221 cagctttgtt cttcccagta acacatggca tatgaataaa cagctcccca ttgattccct
     5281 gctccaataa aataatgcat actatcacgt cgatgttttt ctgcaaatga taagccaaat
     5341 gcatctgctt ttttctctgc ttccactaac cctgttggta aataacgttc gagctgttca
     5401 tagtattctg aataagcttc gaattctcca ttcaaataca tcaatcgatc tgctgccata
     5461 aaaaatttga tttgttctgt tccaggagct ggataagtga ccacaaaatc gcatagctga
     5521 gacaacttac tgttcgcttt atcgataaat ccaatcagtg tcgctcctac tttcttgatt
     5581 tgttctactg ctttcactac ttcttgagtt gtaccggtaa cagaggacaa aataacgata
     5641 gaatcttttg ttaatctttt gtttccagta gtgtaatatt ctgcagcatg ctgtacaaag
     5701 acaggcaagt tgcttttccc attcatataa gtgacagctt gcatcgagga agcatacgtt
     5761 ccaccgattc ctagataata gacagcgtca aatttttccg cataaagttt atcgatgatt
     5821 tcttctactt gcggacgtaa agctagtgcg ccttttattg ctttgatttg ttcttcttca
     5881 ttgaatttca gcatgatttc cacctcttat aatttacttt tcaagactgt atcaaagtct
     5941 tcaaaaatct ttttgttcaa gtcaatacct ttagctactg cggttttata agcgaatagt
     6001 tgaaacggaa cgacaagcgc taatacagac agatattcat cacattctat tgctaatcca
     6061 agtgttttct cgtttcttgc tttttttgtg gtaatggtga gagtctctcc aacgtaccga
     6121 ctcatatatc tttgcaactt ttgcgaacgt tccttattta cactttcccc ttcaataaag
     6181 aatagcaaat gcgaagcatc tgcctccagg tatgggccgt gcatataagc ttctaactca
     6241 aatccttgtg acggaacgcg gattgtttca gtcaatttcg tctctgcttc tttagctgtt
     6301 ccccagtttg gaccatatcc aatggtgacg aaacgctgtg ctaaacgaaa taaactttga
     6361 tatccatcaa aaaattcttc cgttttttga ataatcgctg gtaaatggtt gattattttg
     6421 cgcaactgtt gcatgtagtc ttgttctatt tttttgctga ttttattttt acttattcca
     6481 attcctatcc ctaagagtag taactgtaaa acagtcgcag aatagccttt tgtaacgaag
     6541 cctactgatt ctaccccggc acctaaatca ataatttgtg cagcttctaa agcaagtgga
     6601 ctttgcacgt catttgtgat agcaacaaca ggtaaccctt gtttttgtat catttttact
     6661 gcttcaacag tagaagcact tttcccgctt tgtgacacag caatcacagt atcgattgaa
     6721 ggagaaagtt ttccataatt gaaaaaataa aaaggttctt gaatatcgac agtaagatca
     6781 ccataagatt ccaatgctgg cttcgctgct aaacaagcgt tataagatga acccgttgct
     6841 aagatcagta aattcactgt ttttttcata tgttctcttg tcgaaaaatc attctttttc
     6901 aagatcgtac ttagtacttc cttttcttca tagatataat cttgcatcgt aaacattttc
     6961 tctgctcctt ttttaaaaaa tccctatcca cgaaccaaaa attccaaaga aagctagtcc
     7021 gatcaagatc gttaatggtt tcacttcttt cttcaataga taaaacacta tgccaaatgc
     7081 tccaagacat aagatatttg gaacgatatc atcaaaaata ctttgaacag tcacagcatt
     7141 ttctcctgaa ccgattttta aaggaatatt gatggtgatc atcgtggcag tcattgctcc
     7201 aacgaccatc agtccaataa tagacgcgcc taatgtcaaa ctttccatca taccgtttgc
     7261 ctggatcttt ttcaaaaaac ccgttcctaa gcgatatccc caacctgttg ctagatagcg
     7321 aaacagaatg tgcggaatat tgaaaatcaa aatgaacaag atagggccta aaatatttcc
     7381 ttgcatggct aaagaggtac ccacacctgt ggcaatcaat cgcaaagttc cccaaaagaa
     7441 agaatctcct aaccctgcaa gaggtcccat caaagatgtt ttgatactat cgatcgcagt
     7501 cacatcaaat tcaggatcat ccttattttc ttcctccatt gcagccgtga ttcctaaaat
     7561 cagtgtaacg atatgtgggg tcgtgttaaa aaactctaga tgccgtttca aagctgctgc
     7621 catttcttct ttcgactgat ataacttttt caaaatcgga atcattgcat aaacataagc
     7681 catattcatc tgacgttcat aattccaaga aaactccatt tgaaaagaac gccaaaatac
     7741 gtgatttagc tcttttttcg ttattttgct gtttttgatc tcagaattct tcatcttctt
     7801 ctacctcccc tgcagttgtt tgaagtggta gttgacggac attcataata ttgactacca
     7861 caactgctaa aatcgcacca aaaatagcta ctccggtcac aggaatctct aagtaagaag
     7921 ctatagcaaa acctaagaaa aaatatggtg caacttgttt gttgatcagc aaacgagcaa
     7981 gcatcgcaaa accaagagct ggaatcaacc ctgtagctac tgacaacccc ttttgaacga
     8041 agtcaggaat catatttaat agatttccga tcgtattgct tcccaccata taagaaacca
     8101 ttaccacaac accaagcatc aaggaaagac cgataccggc aagcaagtgc atatgctcga
     8161 ctcctttata atttccttct tctgcataat tgtctgcttt atggttcata attggaatca
     8221 aaagccctaa gtaaatattt ttcaatatca atgtcaacgt agcaatcggc aaacctagta
     8281 aaagcgcggt ttctgtaccg gctccagctg taatagcaaa agccgtccct aaaatcccac
     8341 cggtcacaac atcaggtgga atcgaagcac ctactgagaa cgatccgata aatgctaact
     8401 ctaaagttgc gcccataatg atcccagttt ttacatcacc taacacgatt cctgtaaaca
     8461 gccccgtcac aatcggacga gaaagcaacg atgtccctag tgcatattct gattgtgcga
     8521 caaatgcaac aattcctaat aagactgctt gtacaatcat tgaggttctt cctttcttac
     8581 aaaacttctc ttacatccac ttttttatct gctggtactt gacggatttc tacttctatt
     8641 tgtttgtcaa ttaatcgttt caagcgttcc tcctcttctg gtaaaatatt taccgctttt
     8701 ccgatgctgc gagtaccagg ttttgccttg atccctccta agttgatggt tttgatttct
     8761 ggatacgctt ctgccagctt atatgcatcc tcgatcgatt ccactacgat gaataactta
     8821 tatttatctg tcaccccgct tttcaacgcg gcaatcgagt catcaattga tttgatcact
     8881 agtttgactc cttgtggctt ggctaatttg atcgttgtct ttcgtatctc attcgtaggt
     8941 acatcatcat tagcaatcaa aatacagtct gcgcctattg tttgtgtcca tgaaaaagct
     9001 acttgtccgt gtaataaccg atggtcgact cttgtcaata caatcatttt tcttcctcct
     9061 aaaattcgtc ttcttctatt ttctgattga gccgctcgtt acaaaactgg atcatttttt
     9121 ttgaatcagt tagcacctgc tggatcaaaa cagttgtctc tacttcagcg tccaaccgag
     9181 atactagctc aatcaatagt ggcaaattca atccggcaac gagatagaca tgatcccgat
     9241 tgatatattg taagaattcg ttgttgacac ttccgccaaa aagatccgtc agaacaatga
     9301 tttctttatc tttgtacctc ataaaaagct gccttactgt ttccgctaaa tcaaaattct
     9361 catcagtata gcaatcgaat acagtaatag aatgattttt cccatagatc agctctaatg
     9421 aatctaatat ccctgaagca aattttccat gggaagctaa gataatttct ctatccactt
     9481 tttcatctcc tcttttcact tacacatact aaaaaagcaa gttgcatgcc aaaattagtg
     9541 gcaacaactt gcttatgttc aaaattcttc ttctatcgag agaaactctg tatcttcact
     9601 caaaatatca tagatatagg caatctcaga attggggatc atgacactat aatgctcttc
     9661 aatgacacta aacgcagatt taatagcaga taacccttct ttttgacact gcttccattt
     9721 ctctaatcct tgataggttt cgattggcat ttgtcggatc aatcgttcaa tgagacagct
     9781 cacatggaca tacaacgcta attttttggg attgctgatg cttcgattca actgcacctc
     9841 taattctctc ataaaatgat cgatttctcc catcaccttc tctgtatcta gaatagtcac
     9901 ggaatccagt actttttcca atgaaaaatt acgaatgatg cgctgattga atatttcctt
     9961 ctctgaagca ttcatagtag tgtccaacca ttcagctagt acagacatct tttcccccga
    10021 gatgaggtct tctaatgaaa gatagtcgat attggtgata tcaggatctg tggtgccaat
    10081 cattcctacg acttcgtata tggaaaataa tgggtcactg tttttcttat cttttaattg
    10141 ctgatattca tatgggatga ttttcaactg acaagccgta ggtaagcttt tttctagcag
    10201 ttcacttaag tggaccgccg taccgattcc agtgaagcaa gtagtaatga gtgctttggg
    10261 tttgttttcc tctggataga gcagtcgcca ttctggctgg atctgtcgaa cagtctcttc
    10321 tgctatctct tctaatgata catttttttg caggcattcc cctactgtga ttgctaaagg
    10381 tgtcgtcaca ttattcaaaa tcaaaagcgg tacggataac tgttttttaa aaaactgatg
    10441 gatttctttc agagatccca tgtctactaa tatgatcaat ccatttgaaa catcgctgtt
    10501 ttcgcaataa tcaaggattt ctgaagcaat ctgctgtggt gtgacatcta gtggcatatc
    10561 aaatgattca aatagatcct cttgtaataa acgatttact acatttgcca tactgctagc
    10621 agttgcataa ccgtgagcag caatcatcgc ttttggcagt cgtttttctt ttgaccattt
    10681 cgcttttttt agatagatcg tcagtaaaat ccgatccata cttgtgtctt caatatctaa
    10741 tttgatctta cttaattcca gaattcgttg gacatagtga taactagatg gataagactg
    10801 agaaacttgt tgttctaatt ctttgattag ttggataata tcatgttctt ccggtaacca
    10861 tttgatagtg cttcgctgat agagatagta actcaaggca tagacactgt ttccatcgaa
    10921 tttgatttga taggcagttt ccatttgtcg aaaggtctct cggatagatt gtgtccaata
    10981 aaccagcagc tcgtgttttt cttgtctact ggtctcaaac agcaaaaaat caaataattg
    11041 aatcacttct tgctttaatt ttccttcgca agaatatagc agacagttat ttttcttgaa
    11101 atcaattagg atttgctcaa aacttttaac gattcgctgc tgttcgggct gttgcttctc
    11161 cattaattgc tctaaggtct ggtcttgcgt aatatagatt ccttgctgtg ctgtattttt
    11221 agtaataggc tgcgcaagta gttctttttg caaatgatgg atcgtgacat ggatttcagg
    11281 ctgttcacgc tgttcagcat atgcttttgc tgctgaaact ttgacgatat ttttcagctc
    11341 tcctatgttt ccggataatt tctgacttgt taaaagtgac aaagctggtc cactgactga
    11401 caaagggctc cccatttttc tttgttcttc gagaaaaaaa gagtaaatca gttctagccg
    11461 ttcattacga ctacgctgat taagtgcagg tagttcgatt cggataggta ttcttcgaat
    11521 gaaagttgtt aagaaatgac tagtaatctc ttcagtcgta gcaaaaaaga gacgtgtttt
    11581 gatccggcgt gacaaattag gttctcccat acgataaatc acaccttgat caagataggt
    11641 gaacagtttt tcctgacctt ctggtgttaa gcgatgtacc tcgtctaaaa acaagatccc
    11701 accatctgct gcttcaaaag cccctttttt attttcttct gcaccagtgt acgctccctt
    11761 aacatgtcca aacaaattgc tagtcaaaag ttcaggatta tctgcatatt gtgcacaatt
    11821 aaaagtgatg aatggtgcag aatcttctag cagatcatga agcagacaat actgatagac
    11881 caaattaact aaataacttt tacctgtgcc actttcgcct gtaatcaaaa gtggtaatcc
    11941 gccatctgga tagtaaagag ctgttttgat ttgttcgata ctttctttca aactggcgtc
    12001 gtatccgatc ataaaagaga ataagtcttt tggttgcgcg aaaaaaggct gttcctcttt
    12061 gagatcatcg atagaaaaat aaaaattcgt gcgtaactta aaaaattgtt tttcaaaagc
    12121 ttctttgtgg aaataaatca ctggtcgaga attgattttg actagtagtt gctcatcatt
    12181 taactggttc aaataatgac tgactgtgtt tctttttacc ccaaaatgat cagctaacct
    12241 ttgtgctgta aagacctcgt tcatatgttc taaatcgaaa aaggctgtct ggttctctaa
    12301 gtaacataaa atttccttct tcaacaactt attcacccct cctccatcat agaaaacgga
    12361 ttcattttgt caataaaatc tttaataaag aattgaaaaa tatatcttat tatagacagc
    12421 agcctactta tcccttataa tttttattct aaaaaacgta cagttccttc cagtttttat
    12481 actggaattt caacatatga aagaataagg aggctatgac tattcaatga aatcggataa
    12541 atataaaaac aggaaacata cttcctggaa aacttttcga gaaatcatcg gatcttatct
    12601 cgctgtagtt gtatttttgc ctttgcttta tatggctatg acagaacaaa ctcgttttat
    12661 tggcgtagat acgcaatata tagccgaaac aggattttta ttcatcttta tcttgatttt
    12721 tgcgttctat aaatccttcc atacaaaata agattcacag atttcaactt tcctgtctgt
    12781 ttttagctat tacaaatcct tatatgtggt ataacaacaa tttattggca taatgaggaa
    12841 aaacgagtta tgatgctttc agagagtaaa gaggtactgt tgtcaccatc ctttttattg
    12901 attacgtaca ccactcttag ttttttccca ttgattacga aaacaaggta gaaacattga
    12961 tgttcctacc ttgttttctt tttttatttt cagatcaaaa tctcaagtgg cttttgactt
    13021 gatgtcggga ggttgtcttc gttccatcaa acctgataga atctgcgcca aaccatagtt
    13081 ttctccacta ttatttattc ctctaagata gtacccacaa taaacgaata aatatttttc
    13141 tcattttcat actccttctt tgaaaatcaa ttagatttct ctcaacccac ttttttacaa
    13201 aaattacgta gcaaaaaaga ttgcaagaac aaattaa
//
LOCUS       DAZKOR010000078        13235 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000078 DAZKOR010000000
VERSION     DAZKOR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.079"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..435)
                     /locus_tag="ACG8ZM_002146"
     CDS             complement(<1..435)
                     /locus_tag="ACG8ZM_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0493083.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSG"
     gene            complement(446..1114)
                     /locus_tag="ACG8ZM_002147"
     CDS             complement(446..1114)
                     /locus_tag="ACG8ZM_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFQ0493084.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1245..1829)
                     /locus_tag="ACG8ZM_002148"
     CDS             complement(1245..1829)
                     /locus_tag="ACG8ZM_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0493085.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2164..2394)
                     /locus_tag="ACG8ZM_002149"
     CDS             complement(2164..2394)
                     /locus_tag="ACG8ZM_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0493086.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(2409..3359)
                     /locus_tag="ACG8ZM_002150"
     CDS             complement(2409..3359)
                     /locus_tag="ACG8ZM_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0493087.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3356..4198)
                     /locus_tag="ACG8ZM_002151"
     CDS             complement(3356..4198)
                     /locus_tag="ACG8ZM_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0493088.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(4331..5056)
                     /locus_tag="ACG8ZM_002152"
     CDS             complement(4331..5056)
                     /locus_tag="ACG8ZM_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0493089.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(5517..5831)
                     /locus_tag="ACG8ZM_002153"
     CDS             complement(5517..5831)
                     /locus_tag="ACG8ZM_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0493090.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(5847..7442)
                     /locus_tag="ACG8ZM_002154"
     CDS             complement(5847..7442)
                     /locus_tag="ACG8ZM_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFQ0493091.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(7465..8355)
                     /gene="iolE"
                     /locus_tag="ACG8ZM_002155"
     CDS             complement(7465..8355)
                     /gene="iolE"
                     /locus_tag="ACG8ZM_002155"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010777829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HFQ0493092.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(8372..9397)
                     /gene="iolG"
                     /locus_tag="ACG8ZM_002156"
     CDS             complement(8372..9397)
                     /gene="iolG"
                     /locus_tag="ACG8ZM_002156"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HFQ0493093.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(9421..10428)
                     /locus_tag="ACG8ZM_002157"
     CDS             complement(9421..10428)
                     /locus_tag="ACG8ZM_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0493094.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(10541..11683)
                     /locus_tag="ACG8ZM_002158"
     CDS             complement(10541..11683)
                     /locus_tag="ACG8ZM_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFQ0493095.1"
                     /translation="MPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTGHLQKIK
                     ELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTAKVVAVL
                     AANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKMMMKDLV
                     FMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEAMKKILN
                     TLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPHGISNAM
                     LLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLSIPTIRD
                     YGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(11777..13231)
                     /locus_tag="ACG8ZM_002159"
     CDS             complement(11777..13231)
                     /locus_tag="ACG8ZM_002159"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFQ0493096.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         3953 a         2868 c         2174 g         4240 t
ORIGIN      
        1 accacttgat agcgaaaaaa tgtttttatc taatatattt gaaagatcta ataattcagc
       61 taattcattg attcttttat ccatatgctc ttttgtaaat ccaaagtttt ccatcgaaaa
      121 tactaactca ctcgttgtat ttgttgtaaa gaattgtcct ctaggatctt gacttacgta
      181 gccaatattt tttgatatac tttcttgtgt taaggattgt agcgagttat tttcaaataa
      241 aatttcccca accaattttc cgtcataaag tcttggaata agtccattca ttaactttaa
      301 tagtgtggat ttcccactcc cagaagaacc agtaatcaat gtaagactac ctttttttat
      361 tgaaaaatta aggtcaacaa gcgcttgttc cttaacatcc ttatatgaaa aagaaacatt
      421 gttaaattca aacaattctt tcacctcaaa tcaaaatact taagaaaaaa atagtaacgg
      481 aaaacaggat gatatagtct aataagctca tgtttgtttt atagtaagaa cttcttttca
      541 caggagattc tatccccttt gttattgcat tagcggtcat ttcttcagca atcatagttg
      601 cacgtataag aacgggaact aacagaaatt ccaaatattg cagaggctga aaaaaagaca
      661 agcgaatgtt tctcattttt aaagattctt taatcattct tatttcagaa cgataagtcg
      721 gaaagaatct gatcactact gtaatactta acattatttt tttcggtaaa tgtatttttt
      781 ctaaagagga taataattca ttgctagtat agacactaac caacaaccat gagatcatta
      841 cgaccggcaa cattcgaaat acaatatata aggatgtccc aaaaaatata gtaaaaaact
      901 ctatcttgat agcatcaata attttcatta ctaaaaacag aactgaataa atcaagatga
      961 aataaataca tgtctttatt ttacgaaaca tcaatactaa aactgtagca ataatgaaac
     1021 accccacttg aaatatattt ccctttccta aaaaaataat gaccggaaaa aaaatattta
     1081 aagtaatcaa ggttaacgga ttgatttttg acactctttc acctccaaaa aataagatcg
     1141 cactgttatc ttaagctgat aaaaatgcac cgttattcag ataacagtgc gatcttatca
     1201 aaaagcataa ctgagaacaa ttctcaatgc aagctttttt ttaattaact cagtaattca
     1261 atccacccag gaacagaaaa ttgatacaat cttctggtta atagcatagt cttttctttt
     1321 gaatagctat gcttcaatat atcatagaat gtagcgtaga atgagtaaat gtagaagtgg
     1381 atttcttctt cattaaagac taaattttga ttgatcattc cgatagtctt taacttatct
     1441 agacaacaga ctgtcttttt tgtcacaaat gatgtaattt tttcaacaat atcttgatat
     1501 ttggttccag ctgattggaa taaaaccaaa tcgaatatac gtttattctc aaaaatataa
     1561 ttaataaatg gatctaattc attgaagttc tctctccaac ttttctctat atctatattt
     1621 tctaagttta agaggtattt ggtaaactct tcattataca tattaataag cgcattaata
     1681 tgcggctgaa taatttcttc aaataacgct tccttagaag taaaatgttt ataaaaagca
     1741 cccgtagtta cgcctgcggt caagcacagt tgccttatct tagtttgctg aaatcctact
     1801 ttttcaaaat ggcttagtcc actttctatt aatttttggt gagttgtaat ataatctctg
     1861 gtttctatgg gtacttcctc ctaataagat gaattaacta tattatctta cacaggactt
     1921 gaagtttcaa tcgttaacgt ctattactga actcattcct tagagctaaa ctaatgagaa
     1981 agagcaaaat aactgataca aaaagcaatc ctacaagaat taatagactc atctaaatca
     2041 ttcacctttt ctaaaataat ccgagaaaga tttaaaaaaa ttatatccag ctattaggat
     2101 aagtcctatc ataatcactc caatagccat tttcttatcc tccctaaaaa taaagccttt
     2161 tacttaaaac ctttttttgt aatcgtcaat aaattttttc cctttatcag ttaattgaaa
     2221 tcctatatct aaatagtcct catatctaag tttagtcagc atctcactta cagatgctgg
     2281 actaacactt agaacttttg ctaaatcatt attttttaat ttcttttttc tttttgataa
     2341 ttcctgaatt gctagtaaat aaattagtat agtagttgtt ttaatttgta ccatacctac
     2401 cctccaaatt atttcgaaag gccttcagca attttatcca ggttccattt catcatcgca
     2461 tagtaactat caccatcttg tcctttttct gcaattgaat cagtaaaaat cgttgaatag
     2521 ataggaatat tggtatcttt tgatactgtt ttcattggtc tatcgtccac actgctttct
     2581 acgaataagg agggaacttt tgttgtgcgt aatttttcaa ctaagtgttt tatttgatct
     2641 ggtgttcctt cttcttcagt gttgatttcc caaatgtaag cagaaggcac attatacgct
     2701 ttcgagaaat atttaaagca tccttcactt gtaacaatca tttttttatc attcggaatt
     2761 gaagcaaatt tagatttagc ttctttgtct agagaatcca atttttcaat atacttatct
     2821 agattttctt tatagaattt tttattatca ggatcttttt ccgctaactg tttttcaata
     2881 tttttagcgt aaataatacc gttttctaaa tttaaccaag catggggatc ttccttccct
     2941 ttctcactct gaccctctaa gtaaataaca tctatgccat cacttgctgc aaaatagtct
     3001 ttgttttcct ctttgttcgc attttttact aattttgtaa accaagcatt tcctccagtt
     3061 tccaagttaa caccgttata aaaaatcaaa tctgcttttg aagttttttg aacatcttca
     3121 ggcaaaggtt catattcgtg gggatctttc ccaataggta cgatactgtg taaatcaatt
     3181 ttatcttttg ctatattttc agtaatatct gctaaaatcg aattagtaac tactaccttt
     3241 aatttttcgt tcgaagccac tgtatctttg tctttactat ttgttgaaca tgccccgaat
     3301 ataaaaagag caatcaaaat ccccagactt cccagtactg ctttccattt tctcatcata
     3361 actttctctc cttctttctt aaaaattttt gttttggtga gattagaaaa ctaattacaa
     3421 ataagaatgc cgctgttaaa acaatacttg atcctgctgc tatgttaaaa ctataaccaa
     3481 tgaacaaacc tagcaatgat gagcacgctc cgactgtcgc cgaaataaat atcatagttt
     3541 ttaaactgtt tgcatataaa tatgctgtag cagctggtgt aattaacatt gcaacaatta
     3601 atatagttcc cacactttgc atagctgtta ctgctactaa agttaataga atcattaata
     3661 gataatgata aaaactgaca ttcatcccca ttgcttttgc cattaatgga tcaaaagaag
     3721 tgattaataa ttctttaaaa aatagaacaa ctgtaaccaa cactaaaaca gaaacgccaa
     3781 ttgtaatcca cttatcaata tcttgtacag ctaaaatatt tccaaataag atatgaaata
     3841 aatccgttga gctatttgca actcctataa ggataactcc taatgctaaa aaagaactga
     3901 aagtaatacc aattgctgta tctcctttta ttacactatt gctttttata aaagtaataa
     3961 tgatagaagc taatagacca aatacaatcg caccaataaa aaaattaata cctaaaatat
     4021 aagaaattgc tactcctggc aaaacagcat gagaaatagc atcacccata agagacattc
     4081 ctcttaaaat aataaaacaa cctactgttc cagaaacaat ccctataact acagatgtaa
     4141 ttagagcatt ctgtaaaaag tgaaagtcat acagaccatt aataaaattt tgaagcatat
     4201 tattcccctt gtatattaga gttagggttt taaccttccg gttttgattg gataacccta
     4261 tgaaccatcg ttattttaat ttggtattaa ctcatttctt ttgagataat atatttattg
     4321 gattgagagg ctaagccgtt cccccctctc caataaatat agtatctcca taggcttttt
     4381 ttaaattctc ttcattaaat acagattcta cacttccgtg agcaattaga tttcgattca
     4441 aaagaataat attatcaaaa tacttcttta ccttgcttaa atcatgatgc acaattagca
     4501 ctgtttttcc ctgatgtttc aattctttta acgtatccat aattattcgt tcactaacta
     4561 agtctatccc tacaaatggt tcatctaaaa atatgaaatc cgcattttga gcaagacatc
     4621 ttgctaaaag tacacgttga aattgtcctc cagataattc tcctatttga tgattagaat
     4681 attttcccat atcgaccttt tccaaagcct ttgaaactct ttgccattct gcatttttaa
     4741 ttctttgaaa aactttcatt ccggcatatg ttcccataga tacacattct tttactttta
     4801 ttggaaaagt aaaatcaata tcagcttttt gctctacata tgcaatcttc accagttttt
     4861 tattgatatc ttcattatta atggagacct ctccctgatg agggataata tttaacattg
     4921 ctttaagcaa tgtagatttt ccagcaccat ttggaccaat aatacctgtt aaagcacctt
     4981 cttgtatttt taaagaaatc tcgttcaatg ctaaaaaatc gttataacaa acattaagct
     5041 ttttcacttc taacataact ttctccttct aaatgataat gattatcacg atcatatggt
     5101 atacaaaaat tatattttag tcaaccctaa attttataat ttagattttt ttctgttgtt
     5161 gaagattagg atgtcaaaat tatttgaaac tactcgtata gtaaatagta agtaattttc
     5221 aaaacacgaa gaaaaacaaa aagaaaaaag gaaacgccga agcatttcct ttcagtgatt
     5281 taatgagaac aacacgattt gacttagagt ctcaaaaagt cgaataggta cactcattta
     5341 agtgtctcca ttcgactttt tggggatgag ctggctattg aggtccgctt accctattga
     5401 ataaatacaa gattgggtat ttctaataag tccgcccttc atattggggt gatcggggcg
     5461 aacatgccat cttttcatat accacctcct cgacactcaa taatcaaaga ttctcgttaa
     5521 tcttcttcga tgcttgcaat gatgttttca atattcatat aacgaaaatc ttcttgtttg
     5581 atataaacga tctgagcggt acatttttcc tttttgatga tttcttttac tttttcctca
     5641 gaacgtgcga cctgcggcgt taaaagtaaa acgtcatagt ctttataatt cagtttttct
     5701 tcaacgtttc ggatcgttgc ataatctatc tcatacgata gatttttggc tctgatcgtt
     5761 tcaagcattt ttgatacgag cagcgacgca ctgattccgg tctcgcaaat aatcaataga
     5821 tccttatgca taataccctc ctgtttttag cctgcaaagc caattggtga aaatgccaca
     5881 taagttaaaa taacaatgac taccaatgct atagcagtca ccttggtgta tttccaaggc
     5941 tcgatatcga cctttgtatc gaatgagttc aacacaaatt ctccatcagg tttcaatttg
     6001 gaaaccacca tcaaaatgat aatgtccaga acgaataatg ccgtcaaaac ataaaggaaa
     6061 tggatatttt ggaaaagcat ttttgatagt gcatacaaga cgatatgcac ggcaatcgtc
     6121 gtttttgccc caaaagcagt tgcttttttt gaataaaagg caaagagaat gatcacgagc
     6181 agcggcatgt tgtatagtcc attgaattct tgaacgaact gatacaatcc agccggtgca
     6241 aaagaaatga atggcgctac gataatggag ataatcccca caacgaccgt tactgtttta
     6301 ccagcacgag cgatttgctt atcatttgct ttcttattga taagcggttt ataaaaatcc
     6361 agtgaaaaca aggtcgatgt cgcgttcaaa gctcccgcaa atgatgataa gatcgcgcca
     6421 aagataacag cggcgaaaat accaaagatc attttaggca atacttcact gactaaaaaa
     6481 ggatacgcgt tatctggatt tgacattaaa ctatcgccaa aaatattgcg agcgacgatc
     6541 cctggaaaga caaggaataa cgcacctaag attttgaaga cgccgactag gaaagcgccc
     6601 ttttgtgcct cagctaaatt cttgccggac aatgcttttt ggacgatcat ttgattggta
     6661 caccagaaat acagattgtt aaacagcatc cctgtaaata gtgtcggcca tggaacaaca
     6721 gccgagtcga tcgatcccac agagttaagc agccatggcg tgtttaccac aacgtgttcg
     6781 atcccgccaa caaagctgcc gtcaccaagc ttcattaagc ctaaaattgg gaccagcaga
     6841 ccacaagcca gcagaccgac tccataaatg gagtcactat tggcacttaa cgacaagccg
     6901 ccgatcagca gatagaggat gccgacaata ccaattgcca tcgctacaat tatgatcgcg
     6961 acgatcggct ggacgcccag cagctcatca atatggaaga tcttattgaa caccagcgat
     7021 ccagaataaa gaaccacggg taagaaagaa gtcatatagg tcacaataaa taagatcgaa
     7081 ataattcgtt tggtaaccgt atcataacga atctcgatga aatctgagat tgtattgatg
     7141 ccgtatttaa aatattttgg caagaatatc acagccaaag ctacgatcgc aatcgtagca
     7201 gttacttccc aggccattac ttccatccct gcatgataac tttgaccatt ttgtccgact
     7261 atttgttcgg tggacaggtt cgtcatgatg atcgtgccgg cgattggcaa agcagttaag
     7321 ctccgccctc ccatgaagaa cccttctgat ccagaagtat cgacacttct ggatctagaa
     7381 taggcaaata accatacaca gatgacaatc actaggaaac tgattataga aaaaagattc
     7441 atcgtctagc tccttttcaa tagattaaag accggtgacc tctcgaatgt agcttctggc
     7501 tttgactgca tattcaaatg gattggcttt agccgggtcc tgttctgctt caactacgat
     7561 ccagccttca tagtcgctgg catggattgc tgtccagatt ggagtgaaat caatcatccc
     7621 atcaccagga accgtgaaga cgcctttctt cacgccgttt aagaagctgt ctttttcctt
     7681 ttctacttct tcagccgttt cttgacgaat atctttgaaa tggatgtgtt tgatacgatc
     7741 ttgatgacgg ttgtaaaccg cgattggatt ttcaccagaa aagaccaaat gtcccgtatc
     7801 gaacaataat ttaactttgt ctggatcagt catttccatt aaacgatcga tttcttccgt
     7861 cgtttgaacg ccggtaccca tgtgatgatg gtaaacgatc tccatatcct tttcgtgggc
     7921 caaatcccct aatttttcta atccttccgc cagttttttc cactcttcat cagtgaaaac
     7981 tggtttatca tcaaagactg gcgctgtcat aatcccttgg atactatgac cctgctcagc
     8041 tactacgatg actttagcgc ccatcgcatg taagaaatcc cgatgtgctt tgaaggcttc
     8101 ggcagtttct tcaaaaggtt tagttgtcag aaacgcactg aaccatgcac tggcaacctt
     8161 taagccgcga atattcaaat attgttccaa cacctctgga tcttttggat atttgttgcc
     8221 aatctctgtt ccggtgaacc cagcaagggc catttcactg atacattgtt caaaggtatt
     8281 ttcactacca agctcgggca tatcatcatt ggtccacgcg attggtgcga tggctaattg
     8341 aatttgtttg gtcatattct cacactcctg cttatttttt tagttgtact aaactcttcg
     8401 tatcgaatga ttcttgacaa gccaacgcga tcttagttga ttgcaatcca tcttctgcat
     8461 taaccgctgg ttgacgtttt tcttgaatac atgagacaaa ttcttgggct tcacgaatga
     8521 aggcttctct gaatcgttct gggaagtttt gtgaacatgg acggatgatc ccttgatcgt
     8581 tcatgatcgt cacaagattt ttctcaggcg cttgcgctac ccgcagcatt cctttggttc
     8641 ccatgatctc tgtttcgaca tgatagccat gggctgcgtt tctgccggca actagtaaag
     8701 ctattgtttt atcgctcagg ctcatcattg cggctccagt ttcaagctca ccagcctgat
     8761 ccaattctgg atacgccgca tttttcccaa tcgcccacac ttgatcgact tcttggccgg
     8821 taaaccagcg aaccaaatca atatcatgga tcgccatatc taagaacaag ccgccgctgt
     8881 cgttattcat ggcaaatttg acaaagcttt ccaagccttc gctcggatcg atcccataac
     8941 aacgaatcag tgtgatttcg ccgatttcgc catcttctac cattttcttc gcatactgat
     9001 acgactcatc atagcggcgc atgaagccta acatgaagac ctgttcagga tgctcttcga
     9061 tgactttttg ggtcttttta atatcttcaa cttcaaggcc taatggtttt tcagtaaaga
     9121 catgcttgcc attttccatc gccaagcgga cttggtcaca gtgaaagcct gatggtgaaa
     9181 caataaagac tgcatcgatc acggaacttt tgaccatttc ttcatagctg gaaaaagtat
     9241 gcttgacgcc taatttcttc tgtgcgaatg ctaattctgt ttcaactaaa ctgcacgcag
     9301 ccgtaagctc acaacaactg acacctgttg ctaaattttg tgcatgtact tttcctaatc
     9361 ttcctaaacc gacgatgcct actctaatgt tgttcatgaa atacctccgt tttctgagaa
     9421 ttactcattg tatagtgctg gtcgttcatc catttgaatc attacttttg tttttgtttc
     9481 acgagactca tgacaagcca tcatggtgat cgctgctaca tacccatccc aagcggtagg
     9541 accgacaacc gcatcatttt tgatcgaatc gatccacagc ttgaattcat aatcataggc
     9601 atccaaaaag cgggtctgcc aatctggtga aacaggtgtg taattttttt cttccgattt
     9661 aattcgtgta taggctgggt ccgtcattcc tacttctgcc gtttcgaaaa ccacatcaca
     9721 tttaatatcg tagccaaagc ggcaattcac aaagatttcc aaatcaatcg ttacaccgga
     9781 ttttgtttga aagatcagca gctgcggatc atgcaaatcg gcatgggagt tgctggtttt
     9841 tttcggcaag atcatttgaa cagattcata atcttcattt agcaaccagc gaagcacatc
     9901 aatctcatga atcgcggtgt tgctgatcgc catcggtgtt gtgtagtttt catctgcgct
     9961 aggatttcta tgggcacaat gcatcattaa tgcttctccg tattgcttcg attcgattgc
    10021 ttctttcagt tcaatgtagc cgcggtcata gcggcgcatg aagccaacct gaaccaattt
    10081 cttcccataa gcaacttccg catcaacgat ccggcggcag cctgctgcat ccatcgctaa
    10141 tggcttttcg cagaaaacat atttcccatg cttgattgct tctaacacat actgctcatg
    10201 ggtcgggtcc caagaagtca caacaacgac atccacatct tcagaagcga tcaatgcttc
    10261 tccggtttca aagaatttag catcgacacg tttggcaacc tctttagcag cttttacatt
    10321 gatatctgag actgctgtca cataagcccc ttgtgttttc tgattgatcc tgtcgatatg
    10381 ctcgatgccg attgcacctg ttccgataac gcctactttt actctcaatt acttatcccc
    10441 ctgttctgtt gtttaagcac acatagtatg cgcttacaaa attattcctg tgtagctttt
    10501 attcttaaaa ggtagagccc tctctttgct ccgcctttta ctacccataa gcttgtacgt
    10561 ataattcctt aatttgacta tgactaggca cctttttatt attttgcgga cttccgctgg
    10621 tcaacgcatc cgcggccatt ttgtctaagg ctgccataaa cgcttgctga tcaataccat
    10681 aatctcgaat cgttgggatc gacagctgct cacagagctc ttggattttt ttaatcagat
    10741 aatccgcagc ctcttccagc gataaatctt cggctgccgg ataaataaat ctggcgatcg
    10801 tcgctaactc agtctgaata gcatcctttg agaattcaaa aaagaccggc aataacatcg
    10861 catttgatat cccatgagga atgtgaaaaa gtgcccccac cggtcgactc attccatgaa
    10921 tcaatgtgac tgacgcattc gaaaaggcaa tccccgcctc cgttgaagct aagctcattt
    10981 gttctctggc ttcagtgtcg tcgctatttt catatgcctg agaaagcgtg ttcaaaattt
    11041 tcttcatcgc ttccagtgaa aatagttgcg ttacttcatt ggctttgact gaaacataag
    11101 attccaaagc gtgacatagc gcatcgactc ccgttgccgc ggttactttt ggtggtacag
    11161 ttttcgtaaa atcagagtca acgattgaga tctgtggcat gaaaacgaga tccttcatca
    11221 tcattttgac tgctgttttt gtattggtaa taactgccac atcggttact tccgaaccag
    11281 ttccagcagt tgttgacact gcaatcagcg gaatgcccgc ttgcagctga tcaaaatttc
    11341 ttgcatgaat cgcttcgaga cttaattgat tagcagccaa aacggctacc accttggctg
    11401 tatccagaca gctgccgcca ccaaaagcaa tgatatgatc acattgatgc ttctgaaaaa
    11461 gcttcagcgc atcataaaca ttttcttgcg tcggttcggc ttgaatgtct gtatactccg
    11521 ctaattgatc atctgttttt cccaacagct ccttgatctt ttgaagatgc ccggtctgtt
    11581 ccataaccgt atcactaatg attaacggct tgttgcctag ttgttctgat tcttccataa
    11641 gaaattggat tgttccgccg ccaattctta aattggcagg cattttcagt tgaatattca
    11701 cagaaaaaac ctctctctgg cttatagcct ataacagaat cctcgaagct ctttaacaga
    11761 gcttcgaagc aaatctttaa atctcacttt gcgcataacg tgctgtaaca actttccgat
    11821 gggtataaaa ctcaacactg tctttgccat tggcatgcaa atcaccaaag aaagagtgct
    11881 tccagcctga aaaggcaaac caagccatcg gtgcaggcac cccaagattg acccccagca
    11941 tcccagcatc aacattttct ctgaagtagc gaatcgctga tgcgttgttg gtatacagac
    12001 acgaaccatt cgcaaattca gattcattcg tcagctcaac ggctgccatc aaatctttta
    12061 cccgcacgat tgatgcgacc ggggcaaaaa tctcatcctg ccagatcttc atatccatcg
    12121 tgacattatc aaaaatcgtt ggtccaacaa aatagccttt ttctggaatg ttttcgcgac
    12181 catctagtac cagtttcgct ccttcatcga cccctttttg gatatagtca aaggtccgct
    12241 gctgtgcatc ttttcgaatc actggtccta aaaagacccc ttcgtccaag ccattgccga
    12301 tcttgatcgc ttttgccgca gccttaaatt tttcaacaaa ctgatccgcg atttcctctt
    12361 ccaaaacaat gacggaggct gccatgcagc gttcaccagc agaaccaaaa gctccgccaa
    12421 taaggtcctt gactgcccga tcaatattgg catctttcag cacaacaaca tgatttttcg
    12481 ctccagtcaa cgcttgtacc cgttttagat tttttgttcc ttcacggtaa acgtattcac
    12541 cgacattttt tgatcctaca aagctgatgc cttttaccag tggatcttgc agcaatgtgt
    12601 tgacaacatc gtgggcacca tagacaacat taaaaacacc gctaggaaag cctgcttttt
    12661 caaccagctc aacaattttc tccatcaaca atggtgtttt ttctgaaggc ttcagcacca
    12721 ccgtatttcc tgccgccaga gccatcggga agatccagaa gggcaccatc attggaaaat
    12781 taaacggtgt gatccctccg attactccga ttgggtaacg ataattcgtg atctcaatat
    12841 cagtcgccgc tgttgataaa gaatctccca tcattaaatt ggtgattccc gctgcgtgct
    12901 ccacattttc gattcctcgt tgaacttctc ccagtgactc ggacaatgat ttcccatttt
    12961 ccagcgtgat caaacgcgcc aattcacttt tatgatctgt taaaagctga tgcagcttga
    13021 acagataacg tccgcgaaca ggaacaggaa cttttttcca agtttgaaac gcagcatcgg
    13081 cagctttaac tgcttcggct gtctctgctt tgctagacaa cggtacattc gccagcgctt
    13141 cttctgtagc cggattcatt acttcttcaa actctgttga gcgactagcg acccaacgac
    13201 catcaatata atttcttaaa aatcttgtca tttat
//
LOCUS       DAZKOR010000079        13025 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000079 DAZKOR010000000
VERSION     DAZKOR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.080"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            410..979
                     /locus_tag="ACG8ZM_002160"
     CDS             410..979
                     /locus_tag="ACG8ZM_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0493097.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            966..1826
                     /locus_tag="ACG8ZM_002161"
     CDS             966..1826
                     /locus_tag="ACG8ZM_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0493098.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2016..2720
                     /gene="yycF"
                     /locus_tag="ACG8ZM_002162"
     CDS             2016..2720
                     /gene="yycF"
                     /locus_tag="ACG8ZM_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0493099.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2725..4560
                     /gene="walK"
                     /locus_tag="ACG8ZM_002163"
     CDS             2725..4560
                     /gene="walK"
                     /locus_tag="ACG8ZM_002163"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0493100.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4557..5873
                     /gene="yycH"
                     /locus_tag="ACG8ZM_002164"
     CDS             4557..5873
                     /gene="yycH"
                     /locus_tag="ACG8ZM_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0493101.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5874..6743
                     /locus_tag="ACG8ZM_002165"
     CDS             5874..6743
                     /locus_tag="ACG8ZM_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0493102.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6798..7607
                     /locus_tag="ACG8ZM_002166"
     CDS             6798..7607
                     /locus_tag="ACG8ZM_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0493103.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7710..8339)
                     /locus_tag="ACG8ZM_002167"
     CDS             complement(7710..8339)
                     /locus_tag="ACG8ZM_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0493104.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8509..9801
                     /locus_tag="ACG8ZM_002168"
     CDS             8509..9801
                     /locus_tag="ACG8ZM_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0493105.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9877..10539)
                     /locus_tag="ACG8ZM_002169"
     CDS             complement(9877..10539)
                     /locus_tag="ACG8ZM_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0493106.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10764..11048
                     /gene="groES"
                     /locus_tag="ACG8ZM_002170"
     CDS             10764..11048
                     /gene="groES"
                     /locus_tag="ACG8ZM_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0493107.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11099..12724
                     /gene="groL"
                     /locus_tag="ACG8ZM_002171"
     CDS             11099..12724
                     /gene="groL"
                     /locus_tag="ACG8ZM_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0493108.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4337 a         2234 c         2803 g         3651 t
ORIGIN      
        1 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
       61 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      121 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      181 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      241 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      301 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      361 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      421 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      481 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      541 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      601 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      661 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      721 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      781 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      841 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
      901 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
      961 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1021 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1081 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1141 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1201 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1261 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1321 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1381 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1441 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1501 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1561 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1621 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1681 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1741 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1801 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1861 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     1921 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     1981 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2041 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2101 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2161 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2221 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2281 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2341 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2401 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2461 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2521 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2581 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2641 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2701 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2761 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2821 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     2881 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     2941 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3001 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3061 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3121 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3181 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3241 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3301 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3361 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3421 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3481 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3541 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3601 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3661 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3721 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3781 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3841 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     3901 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     3961 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4021 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4081 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4141 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4201 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4261 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4321 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4381 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4441 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4501 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4561 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4621 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4681 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4741 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4801 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4861 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     4921 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     4981 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5041 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5101 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5161 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5221 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5281 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5341 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5401 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5461 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5521 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5581 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5641 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5701 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5761 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5821 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     5881 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     5941 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6001 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6061 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6121 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6181 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6241 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6301 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6361 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6421 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6481 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6541 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6601 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6661 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6721 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6781 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6841 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     6901 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     6961 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7021 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7081 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7141 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7201 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7261 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7321 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7381 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7441 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7501 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7561 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7621 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7681 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7741 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7801 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7861 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     7921 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     7981 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8041 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8101 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8161 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8221 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8281 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8341 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8401 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8461 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8521 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8581 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8641 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8701 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8761 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8821 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     8881 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     8941 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9001 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9061 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9121 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9181 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9241 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9301 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9361 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9421 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9481 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9541 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9601 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9661 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9721 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9781 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9841 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
     9901 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
     9961 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10021 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10081 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10141 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10201 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10261 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10321 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10381 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10441 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10501 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10561 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10621 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10681 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10741 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10801 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10861 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    10921 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    10981 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11041 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11101 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11161 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11221 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11281 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11341 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11401 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11461 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11521 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11581 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11641 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11701 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11761 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11821 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    11881 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    11941 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12001 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12061 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12121 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12181 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12241 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12301 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12361 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12421 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12481 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12541 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12601 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12661 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12721 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12781 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12841 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    12901 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    12961 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13021 ccatt
//
LOCUS       DAZKOR010000080        12977 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000080 DAZKOR010000000
VERSION     DAZKOR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.081"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG8ZM_002172"
     CDS             33..656
                     /locus_tag="ACG8ZM_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493109.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8ZM_002173"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8ZM_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0493110.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG8ZM_002174"
     CDS             2152..3033
                     /locus_tag="ACG8ZM_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0493111.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG8ZM_002175"
     CDS             3036..3806
                     /locus_tag="ACG8ZM_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0493112.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8ZM_002176"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8ZM_002176"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0493113.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG8ZM_002177"
     CDS             5117..6280
                     /locus_tag="ACG8ZM_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0493114.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG8ZM_002178"
     CDS             6397..6807
                     /locus_tag="ACG8ZM_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0493115.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG8ZM_002179"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG8ZM_002179"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0493116.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG8ZM_002180"
     CDS             9380..9664
                     /locus_tag="ACG8ZM_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493117.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACG8ZM_002181"
     CDS             9824..10060
                     /locus_tag="ACG8ZM_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0493118.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACG8ZM_002182"
     CDS             10057..10455
                     /locus_tag="ACG8ZM_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0493119.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            11534..12922
                     /gene="ltrA"
                     /locus_tag="ACG8ZM_002183"
     CDS             11534..12922
                     /gene="ltrA"
                     /locus_tag="ACG8ZM_002183"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0493120.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT         4534 a         2182 c         2758 g         3503 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata
    10681 ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc
    10741 gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt
    10801 actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata
    10861 tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca
    10921 cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca
    10981 tatgtacgga tacattttcg gaccgattaa ttttaaccat tctatttatt tgcttaccta
    11041 ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggatgt
    11101 gcgccctgca tgggtgaaaa cttggtggtg aaagtccact acaggcttgg cagtaggaac
    11161 tgttagcgaa agacaaggcg gcaatcgtga ggttgtggct gaaggaagtc ggacgcaaac
    11221 tcctgaactg acgaacagaa actagataca aggctgaatt aggtcggata agtcttcatc
    11281 acaagacaaa gtccaatact gcccgaaacc tatacagtaa atctagcagt tacatgggag
    11341 gaaagttttc actcttaccg ggggagatct catgagggtg ttgtgggcgt tgaatgaaaa
    11401 caaaccagca caacgatcat ggcgacatga tggtgaatca tgagaagtca gccgaggtca
    11461 tagtagtaac gacactgacc gttacgaagg actgaacaat aagaatcttg aataatttag
    11521 gaggtgtgag aagatgcgac gaccgcagaa aacaagaaaa cttggctatc atcaaagagc
    11581 tagtgtggaa cacgaagggt atgatgaagt gcgcagcact tcttgcggtg aacagaggaa
    11641 acaagatggt gtagaaaatc tgtttgggag aattctagat cggaataatc tcaatcaagc
    11701 gtatcttcaa gtagtcagaa ataaaggggc ggctggtgca gatgggatga ccgtcgatca
    11761 actgcttcct tacttgaaag agcaccgcga ggaattacta tccaatttac gccaagggaa
    11821 ctataaacct cagcctgttt tgcgagtaga aataccgaaa ccaaacggcg gtgtaagaaa
    11881 actaggaatt cccacggtta ttgatcgtat gcttcaacag gcaatcaatc aagtgttaca
    11941 accgctattt gaaccagaat tctcagataa tagttatggg tttcgcccaa atcgcagcgc
    12001 acatgacgca gtgaagaaag caaaatctta ttatgaacaa ggttaccgat atgtggtgga
    12061 cattgatatg aaagcctact ttgacaccgt aaatcacgat aagctgatgt atttcattga
    12121 gcggaaagta aaggacaaac aagtcctaaa actgatccgt caatacttaa tgagtggtgt
    12181 aatggaaaat ggactcatca caacaactga agaaggaacg ccccaaggag gcaatctctc
    12241 tcccctgtta agtaacatct acctgaacga atttgatcaa gtgctagaaa atctaggaca
    12301 taaatttgtt cgatacgccg atgactgcaa tatctatgtt agaagcaaac gagcaggaca
    12361 gcgtgtgatg aagaaatcca ttcaatttct cgaagaacaa ctaaagttga cggtaaatcg
    12421 agaaaagagt gcggtaggaa gtccattgaa acgaaagttt cttggattct gcattctgcc
    12481 aacaaagaaa ggtattaaaa ttcgccctca cgcaaaagca aaacagcgtg tgaaaagcaa
    12541 gctcaaacaa ctcactaaaa gaaaccgtgg aagaagtatc caacttattt taaaagaaat
    12601 caagcaacta atgactggtt ggatcaacta ctatggaatt ggtgagatga aagaattcat
    12661 gagggagtta aacggttggt tgaagcgaag aatcagacaa tatatttgga aacaatggaa
    12721 gaatccaaga accaaaagaa aaaatctgat tcgtttaggg atcgataaag cgaaagcgta
    12781 tgaatggtca aatacaagaa aaggtatttg gtcagtctcc aaaagtcata ttcttcatcg
    12841 atcattaaca gacaaagaac tggcccgcca aggatatgag gacatctcct tgaagtacca
    12901 gttcgtacac tcaacttatt gaaccgccgt atacgggtcc gtacgtacgg tggtgtgaga
    12961 ggggcggaag gtcagtt
//
LOCUS       DAZKOR010000081        12904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000081 DAZKOR010000000
VERSION     DAZKOR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.082"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..3658
                     /locus_tag="ACG8ZM_002184"
     CDS             131..3658
                     /locus_tag="ACG8ZM_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0493121.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="ACG8ZM_002185"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="ACG8ZM_002185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0493122.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="ACG8ZM_002186"
     CDS             7446..7787
                     /locus_tag="ACG8ZM_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0493123.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="ACG8ZM_002187"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="ACG8ZM_002187"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0493124.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /gene="pheT"
                     /locus_tag="ACG8ZM_002188"
     CDS             9144..11564
                     /gene="pheT"
                     /locus_tag="ACG8ZM_002188"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0493125.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG8ZM_002189"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACG8ZM_002189"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0493126.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4382 a         2243 c         2739 g         3540 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgac tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taag
//
LOCUS       DAZKOR010000082        12861 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000082 DAZKOR010000000
VERSION     DAZKOR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.083"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..2301
                     /gene="acm"
                     /locus_tag="ACG8ZM_002190"
     CDS             136..2301
                     /gene="acm"
                     /locus_tag="ACG8ZM_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0493127.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2573..3058)
                     /locus_tag="ACG8ZM_002191"
     CDS             complement(2573..3058)
                     /locus_tag="ACG8ZM_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0493128.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3148..3493
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3538..4179
                     /locus_tag="ACG8ZM_002192"
     CDS             3538..4179
                     /locus_tag="ACG8ZM_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0493129.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4198..4833
                     /locus_tag="ACG8ZM_002193"
     CDS             4198..4833
                     /locus_tag="ACG8ZM_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0493130.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4851..5666
                     /locus_tag="ACG8ZM_002194"
     CDS             4851..5666
                     /locus_tag="ACG8ZM_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0493131.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5985..7421
                     /locus_tag="ACG8ZM_002195"
     CDS             5985..7421
                     /locus_tag="ACG8ZM_002195"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0493132.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7610..10057
                     /locus_tag="ACG8ZM_002196"
     CDS             7610..10057
                     /locus_tag="ACG8ZM_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0493133.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10287..11354
                     /gene="rlmN"
                     /locus_tag="ACG8ZM_002197"
     CDS             10287..11354
                     /gene="rlmN"
                     /locus_tag="ACG8ZM_002197"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0493134.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11344..11550
                     /locus_tag="ACG8ZM_002198"
     CDS             11344..11550
                     /locus_tag="ACG8ZM_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493135.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11547..12650)
                     /locus_tag="ACG8ZM_002199"
     CDS             complement(11547..12650)
                     /locus_tag="ACG8ZM_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0493136.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4400 a         2150 c         2740 g         3571 t
ORIGIN      
        1 tataaatatt tgtaaaataa ctatcaattt tttagttaaa agattggaaa tgagtggggg
       61 aggaattgaa gaaaggattt ttgagagatg atatagtagg ttttattttt agttaaaata
      121 ggggagatag aaaaaatgaa aaagtgtaga aatattattt tatcaatgtt atttattatt
      181 actaatataa catcactgat tcctgttcat gtctatgcgg atgcaggcag agatatcagc
      241 agtaatgtca cttcgttgac cgtggaccca acgaatatta cagatggagg aaacataaaa
      301 gtaaaattct cgtttgacga aaaaaaacaa aatatccaac caggtgatta tctttggatc
      361 aattggccaa gtgaaggaaa tattcgcggt gaaggattcc aaaaagaaat cccattaatg
      421 attgaaaata aaaatgttgg aactttaacg gtcagaaaag attctgcgca ggttgtgttt
      481 aatgaaaata ttaaaaacct agactcagta gaaggttggg ggcaatttga gatacaggcc
      541 agaaacgtaa ccgataccgg tgaagaaaat acgggaccat tcaccgtgac gagcggtgat
      601 aaaacagcta ctgtaaatgt gacaaagcca gcttctggtt cttcttctag cgttttttat
      661 tataaaacag gagacatgct acctgaagat actaagcaca ttcgatggtt tttgaatatt
      721 aacaataacg gcacttatgt tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga
      781 caaagattag atccaagtac gtttgaaatt aatcaaatac acttaggtga acagaaggtt
      841 tatagaggag aagagggaat ccaacaattt ttacaagatt tccccagcgc tacatttaat
      901 ttttcagtaa ctgataatta cattgaaata actataccaa aaaactttgt caatcttagg
      961 aaaataatgg tgagttataa gacgatcata gaaaacccag aacaaataaa ttttgagaat
     1021 cattcggaag cttggtttaa agaatttaat aaacctgctg ttgatggaga aagtttcaat
     1081 catacagtta aaaatatttc agcttctggt ggagtcaacg gtacagttag aggcgaattg
     1141 aagattttca aatatatcaa tgacacagag attggtatcc caaatgtgac tttcgaatta
     1201 agacgagcag atgaacagcc tattcaagga cagtctagta tcctgttgac ttcgaatgaa
     1261 cagggagaag ctagtatcaa aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa
     1321 gcacccaatt ggattgattt tgatccacta agttcaaatg aactcaaatt ctccataaac
     1381 gaaaatgata cagagggagt cagtttacct atttataaca agaaaaaggt tacgaacatc
     1441 acagcaacta aaatttggaa cggtggaacc actccaagac cgagtattta ttttaaatta
     1501 ttcagagcaa gtacaaacaa ttgggaacca gttcctgatg cagaaacaaa acgattagac
     1561 aatggaataa cttcagtgac ttgggaagat atccaacagt atgatgatag tggaaacgaa
     1621 tatacgttta aagtgcaaga agtagatcaa aacggaaatg attatgtccc ttcaggttat
     1681 caaaaaattg aaaacgggct agtcgttaca aatgagaata aagaagtcat ttcagtctca
     1741 ggacaaaaaa catgggaaga caatgagaac caagacggca aacgaccgac gacgatcaca
     1801 gtaaatctat tagccgacgg tcaaccgatc caacacaaag aagtaagtga aaaagatgat
     1861 tggcgctatc gtttcacaaa tctgccaaaa tacagagacg ggcaagaaat catctatact
     1921 gtaacggaag acaacgtgcc cgaatacagt acgacgattg acgggtataa catcaaaaac
     1981 agttatacac cggaaaaaac aaatatatct ataccaggta gccatataac accgtggaaa
     2041 aattttccag agaagacaga gagagagaaa attagaaatc tattctctca aaaattgcaa
     2101 atatttgctc atacaggaga atccagcgaa caaagaagca attatattag taagagagct
     2161 gtacctaaac agataacaaa taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa
     2221 tctagttttg ctgtaatcat tggtttattg atagtaacta tctgtgctgg gatattctta
     2281 caaaaatata aaaaagttta gttagaatat aatatttttt gttaaatatg actgaaatca
     2341 aaagaataaa attccgattt ataatcgaaa aaagaagcat ttttatatat gcttcttttt
     2401 tcgtagaatc aataaatatg gatttggcta actatggaag aatactcgta ttagttttca
     2461 gcgttcatac tagttgttac attgtttgat tggccaaaag aatttctgat atattttgtg
     2521 ctaaataaag aaaaagacgc ttgaattggg aaataagaaa actactctat cattacaaat
     2581 tttcataaac tggcagatgt tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca
     2641 taatcaacgt agtggtattg atatttggat gaaagcctac ggtatcttga cgatcaatag
     2701 cagcatcgat cacgacttgg atttttttct ctttgtcgtg catcaatgct aatggagtca
     2761 ctgtacctgc tggaacgcct aggaggtcag gaagctgttc ttcagaaaca aaagataagc
     2821 gcttttcttt taattgttca gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga
     2881 tgacaagata gatttgtttt ccttttcttg atttcaataa tagatttttt acttgcggac
     2941 caggtaggga aaaagggaca tttttgactg acgtaatcgc aggatgatcg actttttgaa
     3001 aagagatacc cagttttttt agtagttcat aagcttcttg ttcggttgca cgactcatcg
     3061 cgcatcaccc tccttttttt attagtgtat cataaaaaag agttgacaaa tgaaaaataa
     3121 tcatgtaaat tacacgtaaa gttaaaacaa atgacatcga aaagaagagt agccgttagg
     3181 tgtttttaga gagtctctgg ttggtggaaa gaggcagcag ttaaccgtga aacacatctt
     3241 ggagtcgatt ttctgaagcg tatagtagga aaatccgggg agcccgttac agctgcggtc
     3301 gttgctagat gaccagataa ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa
     3361 ccacgtgatt aacgtccttt gatcttcgga tcaaaggact ttttgttttc atctgaacgg
     3421 caacgactgt gtgaggtttt ttctgcaagg aaaaaataaa cagaggtgga accacgtata
     3481 atacgtcctc ttggcatttg ccaagaggat ttttttattt taaaggagga aaagaagatg
     3541 gagtatttga ttgaagttat gccgcaatta ttagtaggag cactcacaac attgaaatta
     3601 tttttattta ccttgattgg ctcattacca ttagggattg ttttagcatt aggattgtct
     3661 ttgcatttta agccgctacg ctacttgctt caaacatatg tatgggttat gcggggaaca
     3721 ccgctgttgt tgcaactgat ttttattttt tatgggttgc cgacgattgg gatcgttttt
     3781 gatcgtttcg aagcagcact cattgccttt atcttaaact atgcagctta ttttgcagag
     3841 atctttcgtg gagggataca atcgattcct caaggtcaat atgaagcagc gcaagttttg
     3901 caattaagtc cctttcaaac ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt
     3961 ttaccttctg tagggaatga agttatcaac ttggtcaaag attcttcctt gatctatata
     4021 ttaggtttag gtgacgtaat gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta
     4081 ctgcctttgc taggcgtggc aatcatctat cttacattga ctggtattct aacaatcgta
     4141 atgaaacaag ttgaaggaaa attaaattat taccgctaac tgataggagg aagaaatatg
     4201 attgaattga agaaagtgaa caaagcattt ggtgaaaaaa aaataatcaa agatttgaat
     4261 ctaatcattc cagatgggaa aacattagct atcgtaggtc cttccggtgg cggtaaaacc
     4321 acgcttttac gtatcttggc tggcttagaa agttctgaca gcggagaatt ttatttagac
     4381 ggtgtaccgt tcaacccatt cgagcttaaa atgaaagaac aagttgtagg agtcgtattc
     4441 caagacttcc agctatttcc acatctgaat atttttgaaa acattacctt agcaccaaaa
     4501 atggtattga aacagacaaa agaagaatac accaaaaaag cagaagaatt agcggcttca
     4561 ttagggatta gtgatttatt gaatatctat ccttatcagc tttctggcgg acaaaaacag
     4621 cgggtagcga ttgccagagc attggctatg aatccacggg tattggctta tgatgaacca
     4681 acaagtgctt tagatcctga attacgccag caagtcgagc gtttgatcct atcattgaaa
     4741 gaagatgggg taacccaaat tattgtcacc catgatttag tatttgcaga aaatgtcggt
     4801 gatcaattac taaaagtttc cccaattcat taagaaagga gaagatagag atgaaaaaaa
     4861 ccagttttct attcgctttg atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa
     4921 aaacttcagc agataattgg gcgactatca acgaagagaa aaaaatcgtt ataggtttag
     4981 acgatacatt tgttcctatg ggatttagag ataaagacgg gaagctcaca ggatttgata
     5041 ttgatttagc caaagcagtc tttgaacaat atgggattac ggtagacttt cagcccattg
     5101 actggtcaat gaaggaattt gaactgaaca atggaacgat cgatttgata tggaacggct
     5161 attccaaaac gccggcaaga gaaaagaaag tgcaatttac aaaaccttat atggaaaacg
     5221 atcaagtgct gatcacacct aaagaatcag gaatcacaaa ctttggacaa atgaataata
     5281 agagattagg tgcgcaaaat gggtcatctg gttatgatgt atttacgaaa caaccagaag
     5341 tattaaaaga gatagtcaaa aaccaagatg ctgttttgta tgatagtttt aatgaagcat
     5401 tgatcgatct gaaaagcaga cgggtcgatg gtctgctgat ggataaagta tacgcagact
     5461 attatttgaa acaaagaaac gaactagaag acttcaacat aacaaaaggt tcttatgaga
     5521 gtgaggattt tgcagttggt gcacgcaagt cagatagtac attagtgaaa aaaatcaatg
     5581 aagccctaga tcaactagag aaaacaggtg aattccaaaa aatatccgat aaatggtttg
     5641 gcgaagatgt tacgccgaga aattaacaaa taggggactg tgacaaaaga cgtgtcacag
     5701 tccctttttc cgaataaaca gtgttctcct gccaactcct tgtgactcgg agctgaatgg
     5761 caggctcaca acctcttggt aaacggtgtt caaaagccag tttttcggta ttaagccaaa
     5821 tttatgcaaa aatgtagaaa gccattttca caaaattttt cttactactc gaaactaagc
     5881 gactttttca caacctcctt tacataattt tattttgttg tggaaggggt ttcgtgttat
     5941 tctaaaagag gaacgattga aggttatagg aaaggaagag atgaatggaa cacaaagaac
     6001 ttacctcatt tccaaaagat tttctgtggg gctctgcttc tgcagcgtat caagtagaag
     6061 gagcttggca agaagatggg aaaggagaat cagtctggga tcgatttgta cggatacctg
     6121 gaaagacatt taaaggaacg aacggtgatt tggcagtaga ccactatcat cgttataaag
     6181 aagatattgc tttaatgaaa gaacaaggtc tgaaagctta ccgcttctct gtagcttgga
     6241 cacggatttt tcctaatgga cgaggagaag tcaatcaagc tgggctcgct ttttatgaga
     6301 gattgatcga cgaattgatt gaaaacgata ttgaacctat gttgacttta tatcattggg
     6361 atcttccaca agcattgcaa gatgaatata atggttggga atcacgtcag atcattgaag
     6421 atttcactca ttatgcagaa acattatttg aggcattccg cgggaaagtc cattactgga
     6481 ttagtttgaa tgaacaaaat atcttcactt cattaggtta tttgttagca gcacatccgc
     6541 caggtgtgac agacccgaaa cgaatgtatg aagttaacca tattgctaat ttagcaaatg
     6601 catctgtcat caataaattt cacgagttag aaatgcctgg aaaaatcgga ccaagctttg
     6661 cgtatacgcc aaattatcca attgacagta atcctgaaaa tgttctggct gctgaaaatg
     6721 cagaagacct gatggccaat tattggatgg atgtctatat gtggggaaaa tatccgattg
     6781 ctgccatgag gttcttagaa gaaaaaggat gggcaccaac gattgaagct ggcgatgcag
     6841 aattgctaga atctgctaaa ccagattttc ttgggatcaa ttactatcaa acggcgacga
     6901 atgcattcaa tccattaaac ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag
     6961 gttcatcaga agaaactgga gttcctggaa tgtataaaaa agttgagaat ccatttgtcg
     7021 aacgaacaaa ttgggactgg gagattgacc cagaaggatt aaggatcgga ctgcgtcgta
     7081 tcacaagccg ttaccgcatg ccggtactga ttacggaaaa tggcttagga gagtatgaca
     7141 agttgacaga agataaacaa atccatgacg actatcgaat caattactta caaagccacg
     7201 tcaaagccat caaagaagca atttcggatg gagcagaagt cctaggctat tgcacctggt
     7261 cttatacaga tttgcttagc tggctgaacg gttatcaaaa gcgatatgga tttgtatatg
     7321 ttgatcaaga cgaaacacaa gaaggatctt tggaacgata taagaaagac agttattact
     7381 ggtaccaaaa actgattgaa gaaaatggag agaaccttta gttcacatac ttaaaacaag
     7441 aaggtacggc tggatatggc cgtgcctttt cttgataaaa tcatttagaa attaagaaaa
     7501 actgtaaaat aagctgaaaa atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt
     7561 cccttacttt tttttgtata atgaaacttg atgaaatgag ggataataaa tggcacaaaa
     7621 gaaacggcct actaaaaaaa ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca
     7681 ggaacaaatg ctgacgattg gtgttgggtt tgccttgatt ttatttgctg tcttcggctt
     7741 tttaaaatta ggttttttag gaatattgat tgcaaacggc tttcggatca ttgcaggaaa
     7801 tacttaccaa atcctctgtc ttttattagc cgtattagga ttttggattg tcattaagaa
     7861 tacagagttt tcaattggta aaaaccgacg ctggtttggc ggcatactct tttatttcgg
     7921 tatcttatta ttgttacatg cccatttatt tggcaaactc catactgggg aaccaaatat
     7981 catggggacg acatgggatt tgttagccag tgatataaaa caaagtcaag tagacaataa
     8041 cgttggtggt gggatgattg gagcaatcct ttatcatttt acttacttct tgattgcaca
     8101 gcctggaagt tatttagcag cagtgctatt actggcagga ggagcattcc tgttcagtaa
     8161 tctggaaggc tatcagctgt taaatggtat ccaatcaatt ggagaacgag tacaggaatt
     8221 attggaaggt gatcctgcca agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa
     8281 acagcgcgca gaagcgaaag aagcaaggcg cttagctgca aaagaagctg cagaaaaaga
     8341 agctgcagaa tatgagaaga aaaaagcggt gagccagcaa agggaaaata ctgctgatga
     8401 atggcaggaa ccagaaaata cagagccgga gcagctaagt tttgttccaa tcgacagttt
     8461 tcaagaaaat atccatcctg cgaatctaga gaaaccagta ccagatacac caaaacagac
     8521 caatacagca gaaggttttt cagatgagtt gccagaggat gacggtacct cattagaatt
     8581 tgagattgaa gcagaacagg aaaatcaaga ctatgaatta ccgacagttg atttgcttga
     8641 ttctatacca actgtggatc aaagtgatga gtataaaaaa attgaaaaaa atatcggcgt
     8701 attagaacaa actttccaaa gtttcggtgt cgatgccaag gtcgtcaaag ccagcttagg
     8761 accatccgtc acaaaattcg aagtccagcc ggctgtaggg gtaaaagtaa gtaaaattgt
     8821 gaacttaaca gatgatatcg cgcttgcttt agcggcgaaa gatgtccgga tggaagcacc
     8881 gattccaggt aaatcactga ttggtattga agtaccaaat ggaaagatca gtatggtttc
     8941 ctttagagaa atcatcgaag cacaaccaaa ccacccagat aaattactag aagtaccttt
     9001 aggacgtgat gtttctggac gggtacaaac ggctgatctt tcgaagatgc ctcacttact
     9061 cgtggctggt tcgactggta gtgggaagtc agtagcaatt aatgggatta tcacaagtat
     9121 attaatgcgt gctaagcccc atgaagtaaa actgatgatg atcgatccga aaatggttga
     9181 attgaatatg tacaacggca tacctcatct gttgacgccg gtcgtgacga accctagaaa
     9241 agcagcgcaa gccttacaaa aagtagtaca agaaatggaa gaacgatatg aaaaattcgc
     9301 ggcaaccgga gtaagaaata tttctggtta caatgaattt gttcaacaga aaaacttgga
     9361 aaacggaacg aaacatccaa ccttaccatt catcgtagtg atcgtagatg aattagctga
     9421 tttgatgatg gtagccagca atgaagttga agatgcgatt attcgtttag cgcaaatggc
     9481 tcgggcagcg gggattcata tgatcctagc tactcaacgt ccaagtgtgg atgttatcac
     9541 aggaatcatc aaagcaaatg taccttccag aatggcattt gcggtatcaa gcggtacaga
     9601 ttctcggacg atcattgatt ctaatggggc agaaaagctc ttagggcgag gagatatgct
     9661 attcttgcca atgggtgaga ataaaccgat ccgtgtgcaa ggagcattca tttctgacca
     9721 cgaagtagaa cgggtcgttc aatttgttac agatcagcaa gaagctcatt atgaagagaa
     9781 aatgatgccg acagatgaag ttgaaacgac aggtgctcca gaacagccac aagatgagct
     9841 gtttgaagaa gcaaaagcac ttgtcgttga aatgcaaacg gcaagtattt cccttctcca
     9901 aagaagattt cggatcggtt acaaccgagc agcacgttta gtagatgaac tggaagctca
     9961 tggagttgtt ggcccgtcag aaggaagcaa gcctcggaaa gtattcattg agcaggaaga
    10021 aacatcagaa acgttgccgg acgataatga catgtaaaac ttgtgtgatg aagggttgaa
    10081 aaacgaataa ccaataaaaa gacaaggcat gatgatacca aaagaaaaaa tggttttttt
    10141 ccatcttttc taatacggta tgatgtcttg tctttttagt ttttgtgaga ttaaagttct
    10201 attcgattta tttgtgaagt attgcattaa ggaaatgaat catgtatagt atgtagcgaa
    10261 actttgagaa aaagagggaa aatactttgg aaaaacagtc catttatggc ttgacgaatg
    10321 aagaattaat caattggttt atagaaaacg gcgagaaaaa attccgagct gcccaagttt
    10381 gggaatggtt gtatcaaaaa cgtgtaagta atttcacaga aatgacgaat ctatcgaaac
    10441 aattgatcga aaaattatcg gagcatttta tcatcaatcc tctaaaacag atggtcgtcc
    10501 aagaagcttc ggatggtaca gtaaaatatt tgtttgaact ccctgataaa aacatgattg
    10561 agactgtact gatgagacaa gaatatggct tgtcagtttg tgtgacgaca caagtaggat
    10621 gtaatatcgg ctgtacgttt tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg
    10681 ctggagaaat tgttgcacag atcatgatgg tccaacatta ttttgatgag cgcaatcttg
    10741 gcgaacgcgt ttcacatgtc gttgtaatgg ggatcggtga gccatttgat aattacgaca
    10801 atgtaatgga ctttttgcat atcatcaatg atgccaaagg attggcaatt ggtgctcggc
    10861 acattacagt ttccactagt ggattagcac acaaaatcaa ggaattcgct aataacggct
    10921 tgcaagtcaa tctggctatt tccttgcacg cgccgaataa tgaggtgcga acatcgatca
    10981 tgcggatcaa ccgaagtttc ccaattgaga aattgatgga agctgtggat gagtatttag
    11041 agaaaacaaa ccgtcgaatc acgtttgaat atatcatgtt gaatcaagta aatgatagac
    11101 cagagcatgc gcaacaatta gcagatctat taaaagataa gaaaaaatta gcatatgtca
    11161 acttgattcc atataatcca gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg
    11221 atgtattgag attttatgat gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag
    11281 aacatggtac agatattgat gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa
    11341 cagttgccaa ataatcgtat cctactcgtt ttttcaatta ccataaaagg agcagttttc
    11401 ttaattaagg aactgttcaa cttaaataag agaaaagatt ctatagcgaa agctataaag
    11461 ctttttttga caagaaaaac cctcatcaag caaagaaatg tcctcaattt gatgacagcc
    11521 tgcttgatgg gggttttagc aattatttaa ctgattttct tatatccttg ctggctttct
    11581 tgtgtagcat gataagtttc tacgtcaaga atggcctgtt taaatttttc tcgggtgatt
    11641 tcataaggca catgtttaat atcatttgtt gccatggctt tatctaagat tgcttctaaa
    11701 tcggtcgtga cattgagatt catgtcggtt aacgatactg gaagttggtt ctttttcata
    11761 aacacataga ttttatcaaa caaatcgaac tgctgatcca tcatcaataa ggtcagtaca
    11821 ccataactga cagatgctcc gtgaaggtat ccctccattt tttctagaac agtcgtaccg
    11881 taatagaatg agtgtgccat agcagaatta tagttattgt taactaaaac agaaaccaat
    11941 ccggtagtaa caatgatatc cgaaacgact tgttcaattg catatgaggg acgatcgtcg
    12001 gcactatctt tcaatgcttg cgtaccataa gtcaagagac gatcaatgca catttttccg
    12061 atttggattc ctaatctatc cgtgtagcct agatcatcgc cacgagcaga aaaagtagat
    12121 tcgacttctt tggataaagc atcgccaata cctgcccata agtatttatc aggtgcttcg
    12181 gcaataattg ctgtatcaat gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg
    12241 tccattacgc cttcttcgtt atagatgaca cataccgcag tgacgggcga acagttcgaa
    12301 ccaatagtgg gaaaggtgaa gactggttta gctagttttt caccaatgac ttttacagta
    12361 tcgcaagcac ggccaccacc gactgcaaac aggacgtctg ctttttggac actttcatgc
    12421 tggatcaact gttcaatatt tccataagtt gaattacccc cgtaccagag actggcgaca
    12481 acctctattt cctgtgtcaa agctactttt attttgtttt ctgcctttga aagtgcagtc
    12541 ttccctccaa tcagcactac ttttgtccca tattctttga cgattccatt tgctttttca
    12601 tacgcatctg ttccaatggt ataatttggt aagaaaacac tttgactcat attcatttcc
    12661 ttctttcttc gttgaaacgg gttaaaattt taaagagtga tcgatctgac tgcggctgta
    12721 gatggagtag aggtgaacct aagacaggat aagtgatgaa taaggtggca tgaacacctt
    12781 gaaatcatca ataagtatcc taaaaacgaa caagtacaaa aacattgaaa acgacaataa
    12841 tgaatttttg ttcctcttag t
//
LOCUS       DAZKOR010000083        12738 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000083 DAZKOR010000000
VERSION     DAZKOR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.084"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..540
                     /locus_tag="ACG8ZM_002200"
     CDS             97..540
                     /locus_tag="ACG8ZM_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0493137.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(546..1730)
                     /locus_tag="ACG8ZM_002201"
     CDS             complement(546..1730)
                     /locus_tag="ACG8ZM_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0493138.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1998..2696
                     /locus_tag="ACG8ZM_002202"
     CDS             1998..2696
                     /locus_tag="ACG8ZM_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0493139.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2703..3380
                     /gene="nth"
                     /locus_tag="ACG8ZM_002203"
     CDS             2703..3380
                     /gene="nth"
                     /locus_tag="ACG8ZM_002203"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0493140.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3506..4039)
                     /locus_tag="ACG8ZM_002204"
     CDS             complement(3506..4039)
                     /locus_tag="ACG8ZM_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493141.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4336..6717)
                     /locus_tag="ACG8ZM_002205"
     CDS             complement(4336..6717)
                     /locus_tag="ACG8ZM_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0493142.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACG8ZM_002206"
     CDS             complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACG8ZM_002206"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0493143.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7418..7957
                     /locus_tag="ACG8ZM_002207"
     CDS             7418..7957
                     /locus_tag="ACG8ZM_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0493144.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8047..8466
                     /gene="gpsB"
                     /locus_tag="ACG8ZM_002208"
     CDS             8047..8466
                     /gene="gpsB"
                     /locus_tag="ACG8ZM_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0493145.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8488..8855
                     /gene="rnpB"
                     /locus_tag="ACG8ZM_002209"
     ncRNA           8488..8855
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8ZM_002209"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9064..10230
                     /locus_tag="ACG8ZM_002210"
     CDS             9064..10230
                     /locus_tag="ACG8ZM_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0493146.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10276..11772
                     /locus_tag="ACG8ZM_002211"
     CDS             10276..11772
                     /locus_tag="ACG8ZM_002211"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0493147.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11853..12536
                     /locus_tag="ACG8ZM_002212"
     CDS             11853..12536
                     /locus_tag="ACG8ZM_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0493148.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12545..>12738
                     /locus_tag="ACG8ZM_002213"
     CDS             12545..>12738
                     /locus_tag="ACG8ZM_002213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493149.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4273 a         2230 c         2630 g         3605 t
ORIGIN      
        1 tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa taggaaacaa
       61 aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca actgctaata
      121 gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga aatggaaaaa
      181 gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg gaaaatccct
      241 gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc catcagtaag
      301 ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga agagaataaa
      361 aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag acacgagcaa
      421 ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc aaaagaagaa
      481 tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag tagcaaatga
      541 agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa tccgaatacc
      601 gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc
      661 gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt atctgagaca
      721 gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa accataacag
      781 ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt gatgaataga
      841 taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga aaattttttg
      901 agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat caaagagaag
      961 cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg agaaaagata
     1021 gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa aggaataacg
     1081 gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa
     1141 tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc ataaacggca
     1201 tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa ctcagctaat
     1261 agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc catccatcca
     1321 aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata taaggtggag
     1381 atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca tatcgaattt
     1441 cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat aaggatcgga
     1501 tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa ggcagctaga
     1561 gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga gtgagaataa
     1621 ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc agtgaataag
     1681 acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat gtaaaaactc
     1741 ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt gtttccttgt
     1801 caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag tgaagatacg
     1861 catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg accgatcagt
     1921 gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt atactgtgat
     1981 tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga caaacagtca
     2041 tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa gaatttttat
     2101 ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat ttgcagatga
     2161 ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat cgtctcgtaa
     2221 caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca gatcgatata
     2281 atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa aaaaaatacg
     2341 aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa caagaatttg
     2401 gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa gaagaccatt
     2461 atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag gcttatagtt
     2521 ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac
     2581 aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa gtagaacagt
     2641 cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag gagtagcgga
     2701 atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga atgtttcccg
     2761 aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca gtgatcttaa
     2821 gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt gcctcttttc
     2881 cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga
     2941 ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac
     3001 gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct ggtgtaggca
     3061 gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc gcagtagata
     3121 cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct acggtcatgg
     3181 aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag acacatcata
     3241 cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc
     3301 ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa
     3361 aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta tgaattatct
     3421 tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt aaatgtacat
     3481 actcagtagc taggatggta acttattaca atttattgcg ataaaaataa aagttgttat
     3541 ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct ataaaataag
     3601 tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt gtaataaaaa
     3661 tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg gatttgggcg
     3721 gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa aaagtgagaa
     3781 tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt aaaagtacag
     3841 aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat aataaacaaa
     3901 atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa aaaagatggt
     3961 gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag ccttctttca
     4021 aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg ggattattaa
     4081 gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata aacaagtaaa
     4141 tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta agctaaatgt
     4201 tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac agcttggtac
     4261 atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac gcttgtagac
     4321 gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg gtgtatggtc
     4381 atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag gagcagatga
     4441 agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag acgctggctc
     4501 gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc tttgactagt
     4561 agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct ggacatagct
     4621 ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat attgatcttt
     4681 gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt ctgtatttgt
     4741 cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta cgtgtgacga
     4801 ttctgaagtg acaggcgtca tttttttatt gtaacctgtc cacacggaaa tcgaataatt
     4861 tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac caagttttgc
     4921 atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata acccagcgat
     4981 ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg tgatcatata
     5041 agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt ctgttccgtc
     5101 ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat tggcgaaagc
     5161 cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat cttgttcttc
     5221 cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc caagattttt
     5281 taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg ctggaacatt
     5341 acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat tatcccagtt
     5401 tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag tttttccagt
     5461 agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca tcgtagaacc
     5521 aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg
     5581 acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg tggaggcaac
     5641 ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat catatagttt
     5701 cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt agacattctt
     5761 atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga cgatcttagt
     5821 attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat taacagcttg
     5881 atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg
     5941 tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg cttgaggcat
     6001 cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac caaagtacat
     6061 ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat ttacgtagta
     6121 ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc acgcttcttg
     6181 ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa gtttgatcaa
     6241 ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag agagggcaga
     6301 accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat cttctaccga
     6361 cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt cacgcttttc
     6421 tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag atacggtagc
     6481 gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga aaagtcctac
     6541 acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct tgataaataa
     6601 tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct tcttcacact
     6661 ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat ttgccattct
     6721 tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc aaataaggaa
     6781 tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa gataaaggta
     6841 gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga acactattca
     6901 aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc ccattttgtt
     6961 cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag ggaaaagatg
     7021 ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag acgccattat
     7081 agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca cttctcttag
     7141 gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc gccaacccgc
     7201 gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc ccccgattgc
     7261 cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag gcattcttat
     7321 aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct aggccacatt
     7381 atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa aagtaatcta
     7441 tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca
     7501 agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag ggctggaatg
     7561 ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag tagcggaatt
     7621 gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg attttggtaa
     7681 taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag cagattatgt
     7741 cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc acactcgttt
     7801 tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc ctggaaaaac
     7861 gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg aaattcggct
     7921 gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt tgatttatca
     7981 acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa aactagaggt
     8041 gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga attcaaaaca
     8101 aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat catcaaagat
     8161 tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg attgagtgca
     8221 aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca acaaacagag
     8281 gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa
     8341 cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc agcacaacct
     8401 aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa aacacgtcaa
     8461 ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga cttgatcagg
     8521 ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac
     8581 cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct tcggctatgc
     8641 ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat ccggtggaac
     8701 gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt gtctggaaga
     8761 agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg ttttttctcc
     8821 ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag ggaattaggt
     8881 cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta tttttcaata
     8941 cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta ttcggaattt
     9001 gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat aaggagattg
     9061 tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg gctagaagca
     9121 ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa cggacgtgca
     9181 cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg tacagcagat
     9241 cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac
     9301 gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc tgtagctgga
     9361 aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac aaaaaaagca
     9421 atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact gacggaaact
     9481 ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt aactctggat
     9541 acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg agcaccattg
     9601 aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga ccgtccattt
     9661 tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat aggccataat
     9721 atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt cagtcaagaa
     9781 atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat tgaattagat
     9841 attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa tgcagaagaa
     9901 atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt caaaacggat
     9961 aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg agaagaagaa
    10021 actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa aacatggagt
    10081 aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa agcaaccaaa
    10141 aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg gggaactcgt
    10201 ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc aattggatgt
    10261 ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga aatcaatctt
    10321 ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc tgaaaaagct
    10381 agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc aaaaaaaatc
    10441 ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga attgtcggaa
    10501 gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg
    10561 gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca atggcaaaaa
    10621 gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa tattgaatta
    10681 acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga tgttttgctg
    10741 aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga tcagcttaaa
    10801 gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc tgaaacagac
    10861 tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa
    10921 caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc atttatgact
    10981 ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga tttcaaaatg
    11041 gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga catcgatgat
    11101 aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga atcacaatcg
    11161 ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca atatcctttc
    11221 tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt ttatcgttca
    11281 ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac ttaccctctc
    11341 catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt agatgttgct
    11401 gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg accaacaaat
    11461 gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg ttattttccc
    11521 tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca gcaagaaata
    11581 gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg gatgtcagag
    11641 catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga tgcaacacat
    11701 gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt taatgtatat
    11761 caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct atagatgttt
    11821 tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg atcgctgatt
    11881 atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat accaaaagag
    11941 ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc tttgtgacgc
    12001 ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat gagaacgagg
    12061 caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa cagatctcga
    12121 tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa gcggccgaac
    12181 tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg
    12241 gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt gatcctgaac
    12301 tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc
    12361 atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac ggatatgaag
    12421 aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt ctggaatggt
    12481 ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa aaatgatgga
    12541 ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta aaagttactt
    12601 ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac
    12661 aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt cggaattggc
    12721 aggagaacac cgtttatt
//
LOCUS       DAZKOR010000084        12737 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000084 DAZKOR010000000
VERSION     DAZKOR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.085"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8ZM_002214"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG8ZM_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0493150.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG8ZM_002215"
     CDS             complement(323..892)
                     /locus_tag="ACG8ZM_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0493151.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG8ZM_002216"
     CDS             1078..1980
                     /locus_tag="ACG8ZM_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0493152.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8ZM_002217"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG8ZM_002217"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0493153.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8ZM_002218"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG8ZM_002218"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0493154.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG8ZM_002219"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACG8ZM_002219"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0493155.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACG8ZM_002220"
     CDS             8548..8733
                     /locus_tag="ACG8ZM_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0493156.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACG8ZM_002221"
     CDS             complement(8795..9991)
                     /locus_tag="ACG8ZM_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0493157.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="ACG8ZM_002222"
     CDS             complement(10161..11597)
                     /locus_tag="ACG8ZM_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0493158.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="ACG8ZM_002223"
     CDS             11890..12699
                     /locus_tag="ACG8ZM_002223"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0493159.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2672 c         2250 g         4012 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtctaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgacaat
//
LOCUS       DAZKOR010000085        12671 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000085 DAZKOR010000000
VERSION     DAZKOR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.086"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..577)
                     /locus_tag="ACG8ZM_002224"
     CDS             complement(<1..577)
                     /locus_tag="ACG8ZM_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0493160.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
     gene            719..1651
                     /gene="pfkB"
                     /locus_tag="ACG8ZM_002225"
     CDS             719..1651
                     /gene="pfkB"
                     /locus_tag="ACG8ZM_002225"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0493161.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            1633..3096
                     /locus_tag="ACG8ZM_002226"
     CDS             1633..3096
                     /locus_tag="ACG8ZM_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0493162.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            3093..3551
                     /locus_tag="ACG8ZM_002227"
     CDS             3093..3551
                     /locus_tag="ACG8ZM_002227"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0493163.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            3548..4522
                     /gene="lacD"
                     /locus_tag="ACG8ZM_002228"
     CDS             3548..4522
                     /gene="lacD"
                     /locus_tag="ACG8ZM_002228"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0493164.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            4963..5691
                     /locus_tag="ACG8ZM_002229"
     CDS             4963..5691
                     /locus_tag="ACG8ZM_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0493165.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            5709..6905
                     /locus_tag="ACG8ZM_002230"
     CDS             5709..6905
                     /locus_tag="ACG8ZM_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0493166.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            6898..7893
                     /gene="lacD"
                     /locus_tag="ACG8ZM_002231"
     CDS             6898..7893
                     /gene="lacD"
                     /locus_tag="ACG8ZM_002231"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0493167.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            7890..8831
                     /locus_tag="ACG8ZM_002232"
     CDS             7890..8831
                     /locus_tag="ACG8ZM_002232"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFQ0493168.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            9007..9486
                     /locus_tag="ACG8ZM_002233"
                     /pseudo
     CDS             9007..9486
                     /locus_tag="ACG8ZM_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            9499..10389
                     /locus_tag="ACG8ZM_002234"
     CDS             9499..10389
                     /locus_tag="ACG8ZM_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0493169.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            10376..11185
                     /locus_tag="ACG8ZM_002235"
     CDS             10376..11185
                     /locus_tag="ACG8ZM_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0493170.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            11199..11600
                     /locus_tag="ACG8ZM_002236"
     CDS             11199..11600
                     /locus_tag="ACG8ZM_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0493171.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="ACG8ZM_002237"
     CDS             complement(11646..12596)
                     /gene="yidC"
                     /locus_tag="ACG8ZM_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0493172.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         4055 a         2052 c         2774 g         3790 t
ORIGIN      
        1 atttaaaaaa ttgaaaaata aagacataaa tcttattttt gatgttttta tttgttttct
       61 caccgttcaa agaaacaaaa ataacgctgt ctttcttagt tagtttccga ctgatgcttt
      121 tgataatttc tggatcagta tgcatctgac aattttttcc cgttaattga agttttacca
      181 gcatttcttg gccaatcatt tctgaaaatc ctcgggcaaa gacaaaaacc cggtcagcaa
      241 atttgattct ttgtattgca tcttcaatgt tcccgatttc caacatattg attgtacgta
      301 ccacttcttg ttcatttttt agaatggctc ggcggattcc tttgtctact ttatctaaaa
      361 atgaaaaatt gatgttcttg ttttctcctt cttttagatg atgcttaaac gaagtaaacc
      421 cttcatagcc tttctttttc attgtccgca caatggtggc tgttgagaca tttgcttgtt
      481 cacttagttt cacgatggaa tagttaggga tctctaggat gaggtgttcg ataaagttcc
      541 agaggtactg ctctgtttca cttaatttgt gattcatatt ctttccttct ttcaccaaaa
      601 gttagttaaa aaaagcacga cgagtttata aatgtaaagg ttttctttgt gttgggacga
      661 ttaagaaaac attttctatt tcgcgtattt cataatagaa tcaaggaggt cgttggttat
      721 gtctatctac acatgcacga tgaatttggc aattgatcta tttattgaaa cagatgagat
      781 gcatcctttt atggtaaacc gcacaaaaga ggatgacatt caggcaaacg gaaaaggggt
      841 gaatgtgtca ctagtattaa agatgctaga tttcccaagt acggcattag gtttttctgc
      901 tgggttcaca ggaaagtata tagaagacta tttacagcag aaacaaatcc aaacagagtt
      961 tattgaagtg cctggtatga cgcgtatcaa tgtatttaca caagtcaatc aaacgggaga
     1021 agaatacaaa ttagtgaatc aaggacctga aataccgcag acagctgttc ataatttttt
     1081 aaatcaaata agagcattac aagcagggga ttatctttgt gtttctggga gtttaccaag
     1141 agggctgtct ccaaaggttt taatagaaat cagtcggatt tgtttgatta ttgatagtag
     1201 tgatcaagaa gtcatggatt gtttacccta tcgaccgttt ttgctgaaac caaatgagga
     1261 agaactttct tcttggttcg gacgaaaaat ggttacagat gaagattact ttgtttatgg
     1321 acaacgacta gtagagtcag gcgcagaaaa tgttttgtta tccctcggtg agaaaggagc
     1381 gattcttttt acgaaagaca gggtgattcg aggaaactcg ccaaaaggaa aagttgtcaa
     1441 tacagcatgt gcaggtgatg ccatgttagg cactttttta gcaggataca tgaatcgacg
     1501 atcgcttgat gaaacgttac gtaaaagcat agcagcaggc agctctactg cttttcgtaa
     1561 aggactgact gattttttgg atgtggagga attaagtaaa caaatcaaaa tcaaggaaga
     1621 gaggttcgaa agatgggaaa atatcaacta attgcagcaa ccggttgtcc gacaggaatt
     1681 gcgcacacct acatggcgca agaggcatta gagcaagcag ctaggaaaaa aggcatcacg
     1741 atcaaagtgg agacccatgg acaaattggg atcgagaatg aattaacggc tgaagaaatc
     1801 aaacaggcag atgctgtcat tattgccgca gacaaagatg tccattcaga aagatttgtg
     1861 ggaaaacgag tcattgatgt ttctgtcagt acaggaatca aggatgcaca acggctgatt
     1921 gacgaagcat tggcaggaaa aggtactgtc ttagcagata atgaagctgc tgatacttta
     1981 gaaccagaag cgataagtgg tgctaatttt ggtcgtagca tctataaaaa tttgatgaac
     2041 ggtgtctctc atatgttgcc tttcgtggta gctggcggag tattgattgc tatttcattt
     2101 gccgtttggg gaatctattc ttctgatcca gatcatgcac aatacaatgc tacagcggca
     2161 atggtaaaag cagttgacga tgcagcaatg ggaatgatgg ttccagtttt agcagcttat
     2221 attgcagaag ggatcgcgaa acgtccaggg ttagtcgttg gatttgttgg tggaatcgta
     2281 gccatgaatg gcggtactgg atttttagga ggtattttag ctgggttctt agcaggttat
     2341 tttatcttat ttttgcaaaa gctactgata attttaccta aacaactaga tggcttgaag
     2401 gcaatcgtct tatatccagt tattggtgta gcatttattg gcatcgtgat gtctttgctg
     2461 gcaggtccaa tgaaggctat aaatgaagga atgatgagct ttttagctgg atttgaatat
     2521 tcgaatccac ttgttcttgg cgtgattgtt ggctgtatgt gtgcgtttga tatgggagga
     2581 ccggttaata aggcggcata tgtaacagga acagctcttt tgacacaagg aaacacgact
     2641 tttatggctg gtgcgtcagc agcttgcatt gcaccaccgt tgattactgg ttttgctact
     2701 ttattgtttg gtaaatattt tgatactaat gatcgtaatg caggtttggt taattttatt
     2761 ttaggatcaa ctcatattac ggaaggagct attccgtttg ctgcgaaaga cccgatccga
     2821 gtattaccaa cgatgatgtt aggctcatca attgctgctg tcttgaccta tctgtttggt
     2881 gtccaagtac cagccccaca tggcggtttt ttagtgttac ctgtagtgac gggaaaaatc
     2941 caatgggtta cagcgattct tattggttcg atcgttggtg gagtgatact aggtttgatc
     3001 caaaagaaaa gagtaactga taaagacaag aagagggata ataaagcaac aaatcgtaca
     3061 gttatcgaag aaggaagagc aggaggttta tcatgacaat cattcatgaa gagaacattt
     3121 ttttacaaca gccattagat aatcaagaag aagcatttaa gtttttagca aagcaaacat
     3181 tctgtttagg aatcgctcat gacgaaaaag aagtatatca aaaacttctg gaaagagaaa
     3241 aagaaggtac tacaggaata atgtcaggtt ttgctatccc tcatgcgaag tctcaaacaa
     3301 tcaatgaacc tagtttgatt atcgtcacac tgaaaaaagg aattgaatgg aacagtttag
     3361 atagacagtc agttgatttt atttttgcgt tatttatccc agattcagaa gcaggaacga
     3421 ctcatttgaa attattgtct ctcgttgcac gatcattgat gcgaaaagag gttacagaac
     3481 agttgagagc tgcaaaatca aaaatagaaa tagctacaat attaactaaa gaaataggtg
     3541 aagatatatg aagaaaatga gtatagaaaa acaggaacgt ttgaagcgat tgacggatga
     3601 agctggaatt attagcgcgc tagctatcga ccaacgtggt gctttgaaga aaatgatcaa
     3661 ccaatataaa gaagctacat cagaagatat catccaattt aaacagatgg tttctcagta
     3721 cctgactccg tatgcttccg ctattttact cgatccagaa tatggtctgc ctgcagcagc
     3781 gcttcgagag aaagaatgtg gtttgttgct agcttatgaa aaaaccggct atgatgcgaa
     3841 aatgccaggc cgactaccag atttactgaa tagttggtca gtgaaaaggt taaaagaagt
     3901 gggtgcggat gcttgtaaat ttttattgta ctatgacgtt gatgaaccag tagagattaa
     3961 tgtgcaaaaa caaatagtta tagaacggat tggttcagaa tgcgaagcag aaggcatacc
     4021 tttcttttta gagctccttt cttacgatgc aactattggc gatccgtctt ctcttgacta
     4081 tgcaaaagta aaaccgcata aagtgattga agccatgcga gaattttcaa aagaacgtta
     4141 tcaagtggat gtattgaaag tagaagtacc agtgaatatg agatttgtgg aaggatatgg
     4201 gcaagatact tgttatagtc aagcagaggc atccgcgtat ttctatgaac aatcgaaagt
     4261 gactgaacaa ccgtttattt ttttaagtgc gggagtcagc gctgcactat ttcaagatac
     4321 attgcgtttt gcaaaacaat caggttcgac attcaatggt gtcttatgtg ggcgagcaac
     4381 atgggcgaag ggagtagcac cttttgttac cgaaggcgaa gaaaaagcga gtagttggat
     4441 gttagaagaa ggaaaacaaa atattaaaga attaagtaca ctattagaag aaacagcaac
     4501 ttctatttat gttaacaagt aagagaaagg attgtctgag tgacagattc aaaaaataat
     4561 tagttattta aaaaaatgtt tgtcaaattt tatagaaaaa aagtataaaa ctgcaaaagt
     4621 tatctaaaac taataataac atttgtcatt aataagctat ttttagttgt tgtaaatgat
     4681 attggaagta ataaaacgat agttgtagga atatcatagg gattaatgtg gatcacgctg
     4741 ctcgataagt tagaccattt tgacggatcg cttatgtggt tcgtcttttt ttctgttctt
     4801 ttattggtaa ctggtatttt tgaggtatct ttgtctattc aagatataaa gctttaaaga
     4861 gctgttataa tagctttttt ggtctaaaaa acctttgaca aatgattaat aattcactat
     4921 tcttgtgttg tggttataac cagtaaagaa gaggtgattt ttatggagag ctcaaataaa
     4981 ggaaaatcat tgtattatca actagttgat ttattgaagg atcgtatcga aacacagatg
     5041 actccgcatg ataagctgtt atctgaaaga gaactttgtc aacactatgg ggtgagtcgt
     5101 accaccgtac gtctggcttt acaggaattg gaaaatcaag gttatattta ccgaagacat
     5161 ggaaaaggta cctatgtctc tgatttgtct gataagtcat cgaacttagc tggagcatat
     5221 agctttacag aagaaatgcg cgaaataggg cgcgtcccta agacggtcat tttagaattt
     5281 agacaaatgg aagctaataa gagaattgct aagaatttga acatccattt aggtgaaact
     5341 gtatttaaac ttaaaagatt gcgtttggca gatgaagagc cattgatggt tgagcgttct
     5401 tatctaccgg cgaaactatt tatgggactt gatttgccgt tacttgaaag taaaccgttg
     5461 tatgatgtgt tttcacagga ttatgaacaa caagttcaat tagctgatga agagttttat
     5521 gcaagtatcg tgcaaaaaaa agatgctgtt gtgctacgaa tcgctgatgg cgctcccgtc
     5581 ttacatctga cgaggataac ctataacgtg aaaaatgaga ttatcgaata tacagatagt
     5641 gtagcaagag cggaccagtt tcattataaa attcggcata tccgaaaatg attgagaggg
     5701 agaacaacat gtttgagcta tcgaagaaca aattaaaaca aataggtgca gcaatcacga
     5761 cacaagaaat aaaacaacaa ccagatctct ggaaagaaac aatcgataga ttgatgcaaa
     5821 gccaagaaaa tttagctcta ttttttgatc aaataaaaaa agaagcaaac ggaagaagaa
     5881 tacgtgttat ttttacgggt gcaggaacgt ctcaatatat tggggatacg ctcttaccct
     5941 atctaaatga acatggagac aatcagtttt ttatcttcca cagttttggc acgacagata
     6001 ttgtagcttc accaaaagaa tttttgtttc caaatgaacc gactttatta gtttcatttg
     6061 ctcgcagtgg caacagtcca gaaagtgtag cggctgtgca aattgcaaat gaacaagttg
     6121 aaacattgtt tcatttgacg attacttgtg ctaaagaggg gatgttggca aaaaacgcac
     6181 aggatgataa acagaattat ctgttattga tgccagagag agcaaacgat gcaggttttg
     6241 ctatgacagg aagttttacg tgtatgttat tgagtgcttt atggatcttt gatcaaacag
     6301 caatggagaa aaaagaaaga tatcttgaac aattagttaa aatcggtcaa gatcttcttt
     6361 caagagatca agaaattcaa aagttattac ctgatttttt tgaacggatc gcttatctgg
     6421 gttcaggaag tttggcaggt gcgacaagag aagcacaatt aaaaatttta gaattaacag
     6481 caggtaaagt tgctacaatt tttgattctt cgatggggtt tcgtcatgga ccaaagtctt
     6541 ttttaaatag cgagacactc gttgtaggtt ttgtgagcaa tcaaccatat acgcgaaatt
     6601 atgacttaga tattttagag gaaataaaag aagatcgggt cactgccaat gtcattgcga
     6661 ttgcgcaacc gggtactatt gatttctcag gtatgactgt gctattggaa gactcggtga
     6721 gtaaattgcc cgatgcctat cttgcattag cgatgattat gactgcacaa atcattgcac
     6781 tctatacttc aatcaaagta gctaataaac cagatacacc atctccaaca ggcacggtca
     6841 atcgagtagt caagggcgtc attattcata agtatgaaac cgaaggagaa atgacagatg
     6901 aatgaagaaa aaaatagggc aatggagaga ctatcaacaa aagatgggat tattagtgca
     6961 ttagcgatcg atcagcgtgg tgctctaaaa aaaatgatca aatcgttggg aaaagagcca
     7021 agtgaagacc aaatcagtga attcaaagaa ctagtatcag ctgagctgac accctatgct
     7081 tctgctattt tattggatcc ggaatatgga ttgccagcag caaaagtcag agcaaaagag
     7141 acggggttat tgttagctta tgagaaaacc ggctatgacg caactacgcc tggtcgtcta
     7201 cctgatttat tagcggattg gtcttctctc agattgaaag aacagcaagc ggatgcagta
     7261 aaattcttac tttactacga tgttgatgaa gcacccgaaa tcaatcattt gaagcatgtg
     7321 ttcatcgaac gtttaggtag tgagtgcgaa ggagaaaatc gtcccttcta tttggagtta
     7381 gtttcttatg acgcacaaat aagcgatagt tcttctccag agtatgcgaa agtcaagccc
     7441 cacaaagtta ttgagatgat gaaagaattt tctgatccaa aatataaagt tgatgtgttg
     7501 aaagtagaag taccagtcaa tatgaattat gtgaatggct attccacagg tgaatttatt
     7561 cacagcaaag aagaggctgc tgccttcttt aaggagcaaa gtgaagcgac tccattacca
     7621 ttcatttttt tgagtggggg tgtttcaaca gaattatttc aacaaacact gatttttgct
     7681 catgaatcgg gttccacctt caatggtgta ttgtgtggac gtgcgacatg gaaagatggg
     7741 gtcaaagcat acgtcgaaaa tggaaaagac ggagctattc attggttaag aacaaatggg
     7801 agagaaaaca tcgagacttt aaataaagta ataaatgaaa ccgctacccc ttggttcaat
     7861 aaaacaaaaa gcaaaaaggt gaagatgaaa tgattacgac agttaccttg aatccttcca
     7921 tcgatatcgc gtatacaatt gaagaattaa acctcgacaa agtcaatcga gttgaaacag
     7981 tcaataaaac agcgggagga aaaggagtga atgtggcgag ggttctaaat ttattaggag
     8041 aagaggtgat agctactggt ttaataggtg gccagcacgg tgaatatcta aagaaccagc
     8101 tagatcagtt agggatcatg catcagtttg ttccaattaa aggagagaca agggattcta
     8161 ttgctatcct gcatagttcg agacaaacag agcttttgga acgaggcccc ttcataacaa
     8221 agaaagaaca acagacattt accgatcatt tcgagcagct cctcgaaata tcagatatta
     8281 tgacgatctc aggtagttta ccccaaggat gccctcttga ttactataag caattggttg
     8341 ctttagctga taaaaaaggg aaagacatat tactagatac ttctggtaaa tttttgcttg
     8401 aagtattgga aggtaaggag aagccactac ttatcaagcc caatctggaa gaattaggag
     8461 aactgattgg ccaacgtatc tgttcttcga ttcaattaga aaaaattcag tccttattag
     8521 ctcatccaat atttgaagga gtgccatggg ttgttgtttc tttaggagag agaggtgctg
     8581 tggcaaaagt tgctcagtgt ttttacgaag tcattattcc taaaatatca gttgttaatc
     8641 ctgttggttc aggtgacgcg acccttgcag gatcggccca tgcgttatca aaaaggcaat
     8701 caatcgaaga gattctcgca ttttctatgg cttgtggtat ggcaaacgcg caacaagaga
     8761 aaactggctg gatcgaccct atgaaagtta cgatgttgat gaaacacatt caagttaaga
     8821 aaatcaaatg aacttaaata taaaagaaag cgttttcttt agttgggaag gaatttgttc
     8881 ttagcttaaa aatgaaatat aagaaagaaa aaatgaaaac gttatctttg aaacagaagg
     8941 gatatttgat gatgtcacag ttttaattga acaataaata gaaaaaatag ataaggagaa
     9001 tgcatgatgg caattattgg tgtaagaatc gatggacgtt tgatacatgg ataagtagca
     9061 aatctttgga caacgaaact gaatattagt cgaatcatgg tcgtggatga tgaggtagcc
     9121 acaaacacaa ttgaaaaaag tggattgaag ttagcgactc cagcaggggt aaaattaagc
     9181 attttgccaa tcgaaaaagc agcaacaaat attttagctg gcaaatatga ctctcaacga
     9241 gtattgatcg tagcaaaaaa gccagatcga atcttatctt tagtagaaaa aggagttcct
     9301 atcaaagaag taaatgtggg gaatatgtca caaacgaatg aaacacggtc cattacaaaa
     9361 tcaatcaatg tggtagatgc agatatcgaa gtatttaaaa aattagatca gcttggtata
     9421 catttaattg ctcaaatggt accaaacgat aaagcagaag actttatgaa tcttttaatc
     9481 aaataggagg aatgatccat ggatattcaa tggtggcaga tcttattatt aacattatat
     9541 gcaggttatc aaattttaga tgaattacag atctattctt caatgagtgc gccagtgttt
     9601 gcagggttat atgcaggttt agtaatgggt gatttaaacg caggtttgat tattggtggc
     9661 agtatgcagt tgacagtttt aggagtgggg acgtttggag gcgcatcaaa aatagatgcc
     9721 aactctggga cgattttagc aactgccttc tcgatttctc ttggaatgaa tccagaacaa
     9781 gcgattgctg ctattgcagt gcctgtagca agcttaatga tccaattgga tattttagct
     9841 agattcgcta acacatactt tgcccatcgt attgaccgtc acgtagaaga catgaattat
     9901 caagcaatcg aacgcaattt cttaatggga gctttacctt ggtcgctctc tcgtatgatt
     9961 cctgtatttt tagctttagc atttggtggt ggattagttg aaaatgttgt ttctgtttta
    10021 aacgggaatc taaaatggct aggcgatgga ctttcagtag caggagctgt tttaccagct
    10081 gttgggtttg ccattcttct gcgctattta ccagtaaaaa aacatctatc ttatcttatt
    10141 ttaggattta cgatcacggc attattagca acgttattct ctaatgttca attattagga
    10201 acaagtgtgg caagcgttgt taaagatctt ccggcaactt tcaattcgct gccgatgctt
    10261 gctgtagcat tgattggtct agcactagca atgatcagtt ataagaatga tcaaaacaaa
    10321 gaccatcaac cattagctag tgagccatca cattctgttg agggggaaat cgaagatgac
    10381 gaaatctaat tacaaattaa caaaagaaga ttttaagcaa atcaatcgaa gaagtttgtt
    10441 cacttttcaa ttaggttgga actatgagag aatgcagggt tctggctatt tatatactat
    10501 cttgccacaa ctaagaaaaa tatatggaga caatacacca gaattaaaag agatgatgaa
    10561 aacacatacg caatttttta atacttctaa tttttttaat acaatcatta ctgggattga
    10621 tcttgcaatg gaagaaaaag agggaattgc ttcaaaggaa accgtatcgg ggctgaaagc
    10681 tggattgatg ggttcctttg cagcgattgg agattccgta tttgctgctt tgattccaac
    10741 gatttttggg gctttggcgg cgaatatggc aactgatgga aatccgatag gtgtgtttat
    10801 atggattttt gctcagatcg ttgtcatggt ttttcgttgg aagcaacttg aatttgcata
    10861 caaagaagga atttccttag ttactactat gcaatctcgc ttaactgcat taacggacgc
    10921 agccacttta ttaggggtat tcatggtagg ggcattagtt gcgacaatga tcaatgttaa
    10981 attttcatgg gcaccaacga ttggcgatgt gacattgaat atgcaaaact atatcgatat
    11041 gatcttacct aaaattttac ctgctgggat cgtcggtggt gtttattggt tattaggaaa
    11101 gaaaaacatg actcctactc gagcgatttt tatcgtatta attgtgtgtg ttgctttatc
    11161 tgcattgggt gtgatttcga aataagggag agcgaaaaat gagtagacaa ttggtattga
    11221 ttagtcacgg aaaattttgt gaggaattga aaaaaagtac agaaatgatc atggggcctc
    11281 aagaggatat ctacacgtta cctttattac cagaagatgg cactgaaaca tacaaacaaa
    11341 agtttctcac tgtcacagat tcgttaaatg attttgtggt actatgcgat cttttaggtg
    11401 gtacacctgc aaatatcatt tctaaactaa taatggaagg acaacagatt actctttatg
    11461 ctgggatgaa tcttccgatg gtcattgaat ttatcaactc acaaatgatt ggttcagagc
    11521 ccgagtttgt tcgtgcagga tgtgaaagca ttgtctctgt caacgaatta attaatggaa
    11581 tagcagaaga ggaagagtaa caaaaaagat gagggcttag ctctcatctt ttttgtccta
    11641 tgtttttatt tccgcttctg ctgctttcct gcgttgcggc ctttgttgtt tgtactgttt
    11701 tttggttgtt tgatttgttt tgttgcttgt ttgttgttag ctggctttgc tgattctgtc
    11761 acgtcttttc ttggagtgac gacttgtttt ggcgggtttt gtttcaactc ttctgccact
    11821 tgtgctttca aacgtggttt catcataacg tttgtgatga atgtttgcag acagctgaac
    11881 acaccaccga ctacccagta aagtgtaaca ccggctggcg cactgatcga catgaagacg
    11941 atcatagcag gagagacgat tagcatcgta cgcatcgtct ttttctgttc ttcaggaata
    12001 ccgatcgttg agatgtatcc ttgaagcaga taggcgaccc ctgcaatgac aacaagtacc
    12061 atacttggtg agcctaaatc gatgccgtag aaagatgatt cccgaatacc ttctgtataa
    12121 cgtgccgtaa agtacaaggc agagaagatc ggcatttgga tcaatagagg cagacagccg
    12181 attccgccgg tcatactcaa tccgttttcg cggtatactt gctgcatttc tgcttgtgct
    12241 gccatttgtt cttcacgtgt tgttgcagct ttcaattttt gttgggctgc ttcaacttgt
    12301 ggttttaacg cttgcatttt ttcagattgg atcattgttt ttttcgattg actaatacct
    12361 agtggcaaga tgatgatacg gacgatcaca gtcattgcaa taactgccca gccataagac
    12421 caattgaaat tctcaactag atatgtaatc gcttggccca taggatggac gagtactcga
    12481 taaacgattc ccgaagtgtc agggtttccg tttgaatcca tccgcacaca gccagataaa
    12541 aagagcagga tactgacaag tccggatccc aataaccagt ttttccattt attcatagaa
    12601 tacttcctct tctataattt ttacacaaac ttaactatac taaaaaagcg catggaattc
    12661 aatcaacttc a
//
LOCUS       DAZKOR010000086        12399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000086 DAZKOR010000000
VERSION     DAZKOR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.087"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..417)
                     /locus_tag="ACG8ZM_002238"
     CDS             complement(<1..417)
                     /locus_tag="ACG8ZM_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0493173.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDL"
     gene            complement(414..1067)
                     /locus_tag="ACG8ZM_002239"
     CDS             complement(414..1067)
                     /locus_tag="ACG8ZM_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0493174.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1355..1875
                     /locus_tag="ACG8ZM_002240"
                     /pseudo
     CDS             1355..1875
                     /locus_tag="ACG8ZM_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1873..2592)
                     /locus_tag="ACG8ZM_002241"
                     /pseudo
     CDS             complement(1873..2592)
                     /locus_tag="ACG8ZM_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2617..2802)
                     /locus_tag="ACG8ZM_002242"
     CDS             complement(2617..2802)
                     /locus_tag="ACG8ZM_002242"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0493175.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3029..4302
                     /locus_tag="ACG8ZM_002243"
                     /pseudo
     CDS             3029..4302
                     /locus_tag="ACG8ZM_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4409..5440)
                     /locus_tag="ACG8ZM_002244"
     CDS             complement(4409..5440)
                     /locus_tag="ACG8ZM_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0493176.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5447..6277)
                     /locus_tag="ACG8ZM_002245"
     CDS             complement(5447..6277)
                     /locus_tag="ACG8ZM_002245"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0493177.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(6274..7089)
                     /locus_tag="ACG8ZM_002246"
     CDS             complement(6274..7089)
                     /locus_tag="ACG8ZM_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0493178.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(7086..7910)
                     /locus_tag="ACG8ZM_002247"
     CDS             complement(7086..7910)
                     /locus_tag="ACG8ZM_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFQ0493179.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7900..9000)
                     /locus_tag="ACG8ZM_002248"
     CDS             complement(7900..9000)
                     /locus_tag="ACG8ZM_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0493180.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            9178..9963
                     /locus_tag="ACG8ZM_002249"
     CDS             9178..9963
                     /locus_tag="ACG8ZM_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0493181.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9996..10379
                     /locus_tag="ACG8ZM_002250"
     CDS             9996..10379
                     /locus_tag="ACG8ZM_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0493182.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10455..10586
                     /locus_tag="ACG8ZM_002251"
     CDS             10455..10586
                     /locus_tag="ACG8ZM_002251"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0493183.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10555..10791
                     /locus_tag="ACG8ZM_002252"
     CDS             10555..10791
                     /locus_tag="ACG8ZM_002252"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0493184.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            10803..10889
                     /locus_tag="ACG8ZM_002253"
                     /pseudo
     CDS             10803..10889
                     /locus_tag="ACG8ZM_002253"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(10869..11840)
                     /locus_tag="ACG8ZM_002254"
     CDS             complement(10869..11840)
                     /locus_tag="ACG8ZM_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0493185.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3720 a         2788 c         2236 g         3655 t
ORIGIN      
        1 taaatctttc gcatcttttt gcatatcagc tgtagaaaca aattgtagat ttgttttttt
       61 accatcatta agctttattt ctctttcttc aattatcaaa gaattactat cactttctaa
      121 attaacattt atattatctt tgatttgtat ttcattctta ttattttttt cttttatgaa
      181 tacttttatt tcactactct tggaataaaa ctccaaagtt tgttctatat attttatttc
      241 ttttccattc atattactag aaatttcatt tgccatatta tgaatctttt cttttctcgt
      301 ctccaaatat gttttaggaa agataaaaaa aattaatgaa tgaactaata taattattat
      361 tccaagaaca gaaaatattt gtatgaacat ttttggaaat atctttaatt ttttcatttt
      421 ctctccaatt tatatcctac atttcttata gtagttatac agtctaattg taattttttt
      481 ctaagttctt ttatatatac atctattact cgatcataag gagtttcttc gctatctttc
      541 catacataat caataatttg cattcttgtt aacacttgtc cattattatc caataaacat
      601 tttaatactt ctagttcttt tgcatttaca tctatttctt caccatttat tttagctgta
      661 tagctattaa aattaaccga tagatcctta tattcaaact tctctaaatg tccataattc
      721 ttcttaatta aagaatctat tctagctttt aagaccggca atgaaaatgg cttttctaca
      781 tatccgtcta ctaaattagt aaatgcatca attttatatt cttcatcact aaatgcagtc
      841 aagattagaa ttggtaaatt gcttttcttt ctaatttctt tcaacacttc taaaccattt
      901 ataaaaggta tctgaatgtc taagataacc aaatttatat cgctattaaa ttttgacaag
      961 gcttctcttc catctttagc ttgaataaca gtatatccaa attctgaaag atattcactt
     1021 attccctctc ttatcatatt atcatcttca acagttaata ttttcatata tacctccact
     1081 taagcctata cactagatat tatacagcat attatggctc tataatatct agtgttggtg
     1141 acgccttctg ttgccaatac tttttctttt ttttaataaa tgcaaacaga caccttgaaa
     1201 tcctttagaa aaaaggcttc tataatattt tgtgttgttg gaaaatcgaa ggcgattttc
     1261 tactaacact ttttcttcgt ttcaactcaa aaagagactc acttttggta aaatttagtc
     1321 gaccaaaaca tcaccctacc aaaggagtcg ctctatggat aatcatacta gaaaattact
     1381 cggattaaca gataaacatt tattttttga tgaagaatgg ctgattgaaa aagaatataa
     1441 aggcgttcaa gcgcagttta ttaaagggag attggatgac tcaccttccc attgtgaaca
     1501 ctgtggatcg attcgtatta tccgcaacgg ttcctatacg acacgtactc agatgttgaa
     1561 agtcaaagaa aagttgacta ttttagaatt aaaacgtacc cgttttctat gctatgattg
     1621 tgggcagacc ttttctgcga aaacggactt agttgatgaa catcatcaac taacaaagga
     1681 actgaaacag gcgattctca tagaactgta cgaaaatcaa tcacgtaatc tgaaaatcag
     1741 aagaagtacc gtcggctcaa gcgatattgg aaactcttac tcaaagattc cacgacattg
     1801 gagccgctca aacggcatta tcatcgactg tttaaacgac caattagtca aacagaaatc
     1861 gtcgatgaac cgttgtgcac aatggtgtcc tgaacgtaca gcgacacctt ccatatccag
     1921 agcagtcgcc acatcacggg gatggatgcc atccaaatta aaagcaacca cgccagtatg
     1981 atgttcagga tcttgcggtc cgtaaatggt gataccgccg atcgcttgta atttaggtaa
     2041 aacgtaggca accagttctt gctcataacg atgaatattc tccataccaa tctcattcaa
     2101 gtaatcgatt gctcctgcaa tgttcggagt accagcttca aatttccatg gcagctcttt
     2161 ccatgaacta tcgtaaagat tgacaaaatc tatcatttca ccgccgaatt cgactggctc
     2221 catttgttct aacaattgac gtttgccata taatacaccg attcctgtcg gcccgcacat
     2281 tttatgcccg ctaaaggcga aaaaatctgc atccaacgct tgtacatcaa ctgtcatatg
     2341 cggtgctgct tgggcaccga ctggactatc ctcaactaca acagcctgtt caaccctcag
     2401 ccgtttcaag cacaacaaat aaccttctgg atctggtttg ccgacctgaa gatcatcgat
     2461 cgtcacaaag gaatcaattt tttccttaat catcggatgt gcttcaaaaa aatagtctag
     2521 ctcccgcagt tcagaattga gactactccg attttccagt gctgctgatc cgctgctcaa
     2581 aatgttcagc atccactggt ctcccaaccg cttgacttat cccttcataa aaggacagtt
     2641 ctgctgccgt cccaacacct tgttgatagt ctgcaaaagg aattttatag ccgaactgct
     2701 ccagtaaata ggcttcagta aatagtgatg aatactctcg taaaacatcc attcactgtc
     2761 aatcatcgtc ccatcaaaat caaaaataag tgctttggtc atcttctttc ccccttaccc
     2821 aaaacaagca aaagctgtgt cctgctctct ctacagtatc agcataaaaa ctgccgtctg
     2881 aaaagtcaaa ggtgaaagaa atgtggcgct ataatttcta ggactgatag taaatcattc
     2941 tatcagtcct tttgagcata aaaaaacaaa acatacagaa gctccgttag aattgaatca
     3001 ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcttatca aactattact
     3061 aggaatcaaa gatggaaaca ttcaaatcac tcatgtcgag aagaaaaaaa tacattcaat
     3121 caacagtatc gttgtttctg ccacgctcat taaaacgatt cagccctgtc ctcagtgtaa
     3181 acaggtgaat caacatgggc tgatcgttaa gaacggaaag aaaaaatcgc tggtccttct
     3241 caataagtgt gcgaatcaac ggacttatct tgctttggca aagcaacggt acctctgtcg
     3301 tggctgtcac acctatttta cggcgaatac ctacattgtt gaaaaaaact gttttatcgc
     3361 caaacaagta cattataaaa tattggagga attgactgaa aaacaagcaa tgacgacgat
     3421 ttctaaacat tgcggcgttt cttggtcaac cgtttcgcga acactcgctt ctctgctccc
     3481 catgacaaac gtgaaaataa attggttgcc tcgctgttca ttggtagatg aatttcgttc
     3541 attgaaaaat caagtcggtc cctattcttt ttcttgtatg gatggcgata ctgggaaatt
     3601 actggatatt cttccttcac gtaagaagaa agatctcatt tcttatttta tgcaatttga
     3661 acgacgtgca cgtttaaacg taaaaatact cgtaacagat atgaacgcct cttatgcttt
     3721 gctgataaaa gagtgctttc ctcatgccaa actcgttgtg gatcgctttc acatcgtcaa
     3781 gcacctcatt cgcagtttta aggatatttg actatgagtc atgaaaagct tcgaccgaaa
     3841 cgatccaata caagccaaac actatcgaca agtgaaggct ttatctcgac ttctgatcaa
     3901 acgacaggac acgcttgttt atgataaatg gacaaaatgg cgtaattttg gttgggccta
     3961 tttaaccgaa agtgaagtcg tagaacgtct actttctact tctgatgaac tacgaatagc
     4021 ttatgcttac tatcaagaaa ttcttcaagc tttttatgac aaagaagcgg acatcttctt
     4081 tcagcttgta aaaacaatgc ccaagtcggt tccaagagaa ctgcatcata taaaaaaagc
     4141 gtttataaac tatgaatccg gcattcgttt agccttagag ttgccttact caaatggcaa
     4201 aatagaaaat ctgcataccc acatcaaagc attgaaacga atagcttacg ggttaggatg
     4261 agaaccagaa ttttcttaat caatcatctg atcacttatt aaacataaaa aaacatctaa
     4321 agcagaaaat caattctacc ttagatgttc ttatatcact ctatcagtcc tatttgacaa
     4381 agagccagaa atgtttgccg actatacact agctttgttg tgcaaaatgc tgagtatcaa
     4441 tttccacagg gattgctaga atatctttca gctgaatgta taaggcatcg ctaattttct
     4501 cttcccgatc gttcaaccga tccactaaaa tttctttgcc ctccagcgtt tgaaaaacat
     4561 accgttgaat gttgcctaaa ataaatgatt ccttccatag aaccggaatc ttaaccccct
     4621 gaggaaccgg ttcaaattga atcagctccg gtcttaaata ataaatgcag cgattgccct
     4681 tagcggaaaa ctcgtgggta tactgattca gctcatacca ttcgaaacga ttatagttgc
     4741 cgataaattc cgcaacaaat cggctctgag gattctgata caattccttc gaggtggaaa
     4801 cctgagccac ccgcccagct tccatcacaa aaatccgatc actgattcgc atcgcctctt
     4861 cttggtcatg agtcacaaaa atcatcgtca tccctagcga ttgctggata gcgcgcaaat
     4921 cccgctgcag ctgttttcta attcgggcat ccaaagcact taatggctca tccagcagca
     4981 ggactttcgg ttcagtgacc aatgagcgag ccagtgccac ccgttgtcgt tgaccgccag
     5041 aaagaaactg gacattttta tctgcctgct ccgtcatatc caccaatgat agcatctcaa
     5101 aaaccttttc ctgaatcacc tcaggactca gcttctttac cttcaaacca aaggctacat
     5161 tttcaaaaac agtcatagtt ggaaacaatg catactgttg aaaaacaaag ccgatattgc
     5221 gatccttcgt aggcttatcg gcaatattct cattgtccag cacaatctca ccgctctttg
     5281 gttgttccaa tcccgtgata cagcgcagca acgtagactt tccgcacccg ctgggaccta
     5341 gtaaagtaac cagctcaccc ttttcaatct gaatttcatc aatagctaaa atctgttttt
     5401 catcaaaaaa cagattgaga tcatgtattt ctaaaaacat tttccgtcac aactccttta
     5461 agggcttctt tttcgactgc ttctcttccc tcgtctgctt aagggacagc tgttgctttt
     5521 gtcgataagt cagcataaag gtagttaaag caattcccac cataaacagc aaatacaaaa
     5581 ccatcacagc gctagccaaa tggccgtttt cgttcatccg ccgcagcata tagatgcgta
     5641 ccgtctgaaa tcgaccgcca atcaacaggt tcgtcagcat gtactcccca aaaaccgaag
     5701 aaaaaatcat cagagaaact agggtcacac cgatacggat gtttggaaac agcacctgga
     5761 taataatcgt agacatcggg gcgcccaagg tttctgctgc atccactaat tccttcagat
     5821 taattaagcg catctggttg ttaagactaa gatagacaat cggcaacgcc gtaataaaca
     5881 atgcacctac caacactaca aacattggga taccagtttc agcatacgtt cgaagcaaag
     5941 ccgttaccaa aatcactcct ggtaaagcat acggcagcag gagcagcttt tccagccaac
     6001 gattcaaacg agggaaatac aaatgaatcc aaataatggt gggaatcatc aacaacagaa
     6061 tcgtcaccaa gaccacacct gctaataaaa gtgagcgacc caccgcagca ataaattccc
     6121 tgtccgtcag cagctgttgg aaccattgca aggtaaaatc actaggtaaa agcgattttg
     6181 accatttagt ggagctggca tacaaaatcg ttccaataat cggcagcaag taatacagga
     6241 caaagcagaa caaaatcggt tttattaact ttttcacgtc tgcaccacct tgcctttctt
     6301 ttgaataatc aacattgccg ttaccagcag gacacctaaa gcgactgcca acgcactgcc
     6361 taaattaggc ttggcgaaca catctcccga cactaaagcc gcaattcgaa tcgttaaaag
     6421 atttacatta ctgctggtca gggcatacgc tgtttcatag gtccccatgc cattagcaaa
     6481 taagatgaca aaggtgctgg caatcgatgg tcgcaaaaaa ggcaaagcca ccttgaacca
     6541 agcatacgct gatgaagcgc ccagcaattg tgcggactcc agccattcct ttttcacttc
     6601 cttcatgctg ggatacaaaa acaagacccc taacggaatt tggaaatagg tatacgtgat
     6661 ggtcaatccc caccaagaat acaaattgaa gtccgctaga aatggcaaaa tcattttcag
     6721 cacacctaca ttccccaaca aaataataaa agagaatgcc agcggaatcc cagcaaaatt
     6781 agctgccaaa ttggaaacaa aggtgatttt ctcctgcaat ttagtcgata gattcattaa
     6841 gcataaacat aaaataaacg tcccgattaa cccaatcaca ctggagctga cagcaatcag
     6901 tacgctgtta acaaaggcct gtgtgtaata tttgttgctg aaaatatcac tgtaattggc
     6961 caatgtccac tggttagtta aatcactgtg caagctgcac agaaccataa aaagcagcgg
     7021 cagcagtaaa aatgcacaga agataaagga ggcaggaacc agaaccaagc gttttttcat
     7081 ctctttcatc ccctcatcaa ttcttgaatg tcctgcattt tctctgacgg tgcaattcct
     7141 aataaccagc aaaacaatgg agccgccagc agctgtggta cagtttgttc cttcagcttt
     7201 tggcgtttat ttgaaatcaa ataaaaagga acttcacgat gtgcagccaa gctgccgcca
     7261 tgcagtccgt gcgcatccat cccgtgatcc gcagtcacca cgacttgata acctttttgc
     7321 agccattcag gaaggtaaat agccaataat ctgtccgcct gattaaccgc tgctgcatat
     7381 tcatgagaat cggcggtaaa tttgtgaccg acatcatcaa tattcatcga atgtacgacc
     7441 aaaaagtctg gctgatactg attcactaaa taatgggcat ccgcaaataa atgcgaatca
     7501 gggtaagtgt cctcactata aaagatgcca tgctgaatca gtcgctcctc ctgatgctgg
     7561 atgcgatcaa ccaaacgatt atagggggct tgattgtaca actcgctgta ccagtggtaa
     7621 gcggcggctc ctgtttttcc gccagcttcc ttaactaatt caaacagcga ccactctttg
     7681 gatcgctgag catggtaatt actatagatg ccattttcat aggctggaac gccggtcatc
     7741 agtacttcgt ataacggacg tgaattgctg ggcagctcac tttttacctt caataagctt
     7801 gcttgctggt gctcaactaa atgattcatg aacccaagct gggcgacggc tgtgtcgtag
     7861 cgacagccgt ctagtataac tacaatcagt ttgtcaagtc tactttgcat aggtcagtac
     7921 ctccgcctgc caaagctctg gaagctctac agaggtcttg ttccaagctt cattgtcttc
     7981 aactggcacc gccgcttcat aagcatcctc cggcagtaat ttttcagcta cttctttagg
     8041 cagctctacc ttgcgaatag gtcgcgcata gccctttgcc aaattgattt gcccttcatc
     8101 agataaaata tattcccgtg ctagctttgc cgcattggga tgaggggcat ttttattgat
     8161 taaggttgta tacccgctaa tgatggagcc gtctgttggc acagttacct caaaacgctc
     8221 ggagtcaatt tggtcacgat aatttagtcc attgaagtcc caaacaactg ccacatcgat
     8281 ttcacctttt tcaagatttg ctacgctggc atcaacggtg gacaaacggc ctttttctgc
     8341 catttttttg tagtaattca gacctggctg aatatccttt tcgtcacctc cgttggcaat
     8401 cgccgcagcc aatacagcaa attgtgcttg gttggcaatc gtcacatccc ccacgctaac
     8461 ttgatagtca ccctcttgca gctccttcca gctagtcggt gcttgcttca cattctcttt
     8521 atcggtgata aaggaaatgg ttcccgtata gcttaacaac caatccccat catcatcctt
     8581 tgcccaatcc ggaatttcct cccaatagtt ggtcttataa ggtaaggtta actctttgct
     8641 ttcagccaac ggaccgaagc tgataccgac atcaccaata tcggcggttc cattttttcc
     8701 ttctgattca aacttagcca attcttctgc actggacata tccgtatcca cgtgacttaa
     8761 gccgtattct ttttcaaggt cgttccaagt gcctacccaa tttgcccaag tatccggcat
     8821 tcccaagctg gcaatctctc cttcttcctt ggctttttct ataatcgtat ctaaagagtc
     8881 gtctgccgca gccgattgct tcgttgcttc acctgagccg cagcctgtca gcaggatact
     8941 tactgttgct gcacaaaata aaccgatagt catgttacga attttttcat tcattttcat
     9001 tcattcttct cccctttcga taattccatt ctaacaaagc tttgtaatga gagaatcagt
     9061 ttcttgaaaa ggtttcatga acaatgtaaa ggttgtatat ggtttgtaaa tttcgacgaa
     9121 ttcccgttaa ccttctaaag cttttgcggt atgatagtag aaaaaaggag gctccctatg
     9181 cagtattatg atcagcatct ccacacgtat ttttcctttg attcagagga aaaatttgaa
     9241 aactatttag cctatcagcc ggagtttttc gtttctacgg atcactttga tttaaacaat
     9301 ccatctaccg gaaaagatga cattccggat tatccagcct atataaacaa actggactct
     9361 ttaaaagaaa cctattccac caaatttctg cgaggtatcg agattggcgt tgttccagga
     9421 caagaagagc ggatcgtgga ttacctcgct cagcatcctt atgatttaaa gctggtaagt
     9481 attcatcaaa atggccgctt tgattatatg gacgatgtgg ttttaaccaa agatcccatt
     9541 caaactacca aggaatattt tgatcagatg ttgcaggtct tgacggattt tccagaagga
     9601 gatattttga ctcactttga ttatggatta cgccgcttca ccttcacagc ggaagaattc
     9661 gcggagcatt ttgaggctca gctgctaacg attttcaaaa aggttgttga acgagaattg
     9721 gcgatggagt taaatgccaa gagcttcttg aaatacggca acgaagacct ttaccgttat
     9781 gccgttcctt tgtatcaaag tgtcggcgga aagctcttta ccttaggctc ggatgctcat
     9841 gttgccgaag attatcagct gggctttaaa aaaatgagtc agctgttggc agactgtcag
     9901 gtagaaaagt tgctagtcat tcaaggaaaa gaacgccgcc tgacgccgct gccgattttg
     9961 tgattcctat ataatagaag aaagtggtga aaaaaatgag caacctgtgg gactacaatc
    10021 aagaggcacc catccattat ttaattgcgc gacattggga tgcgttgaaa attgaggcgg
    10081 tttgccgatc cttgttagca gccgttccaa aacaacaact ggaaaatttt ttggtggctg
    10141 acagcttgca gcgggaaaaa gttcaggctt attttgcagc ctttaaagat cagcctttgg
    10201 aatatttgca tgcgcagttt catctttttt atcaagtagc cgccccagat gactacaacg
    10261 atttacgagg acagctgcaa ttgacctttc aagcggatga aaccgcctat accgttttat
    10321 tgggaatggc tcgccttggt gatcaggcga aggtcgagtg gcggattttc gatatttgat
    10381 ggcaggatca aggcgtttca gttgcaaacc ttcctctctt tcaattagaa aaaccgcagc
    10441 aggaagaagt ttgtgtgctg gtcaccgatg ctggtgaaat tcgtctccgc ttatttcctg
    10501 accaagctcc cctggctgta gccaactggc gctccctagc tgagcaaggc gtttatgaca
    10561 acacaccttt cggctcgtgt cattaaggaa tttgttattc aaggtggcgc tttggatggt
    10621 tctggtgatg aagctgtttc cagctatgag ggctttttcg ctgatgaagt gcatcgcgga
    10681 ctttatcatt ttaatggcgc cttggcctta ggggaccacg gcccccacac caacggcaac
    10741 cagtttttta ttgtgcaaaa cacaaaggcg caggctgacc tgctaatgta aagcaagcat
    10801 acttggagct tggaggacta ccggagctgg atggtcgtta tactgttttt ggacaggtat
    10861 accaagggtt agaaatcgta gcagcgatcg cagctcagcc gacaaacagt gaagatgtac
    10921 cgattgagcc agtgttcatt cgacaaatta acttcgaaaa aacatcgtaa aaagacactc
    10981 actgacttca gcaaaagctc tattcttgag ctggctgcag ctagtgagtg tctttcattt
    11041 atccataaga taaagcttgg attcaataaa taaataaatg ttttggttaa agctgtgacc
    11101 agcaaatgag gaaccgtgcg agccagttga tctgccaatg caaacatttc tttaggtgaa
    11161 ggcgctggcg tgtctgccaa cggatactcc agtcccaatg cttggtaacc gtgcatacca
    11221 tagcagtggt acggtagtaa ttctaacggt acgaaccgat caagatctgc cacaaaacaa
    11281 gcaatcgtca gcagctcggt caggttcata ttgatacctg gaaccacagg aactcgaatt
    11341 cgcacttcgc agttaggata ctcaattaaa aagcgacgaa tattcttcaa aatttgttgg
    11401 ttatctaagc cggtccattg tttgtggagc ttgctggtaa acatttttac atccacaaac
    11461 aatgtatcca catagggagc taccttttgg atttcattat aagcaccaca aaagctggtt
    11521 tccagtacgg tattgattcc aatgtatttc gatttttgca aaatttcttt agcaaaatcc
    11581 gcctgaacca gtgcctcgcc tccgctaatg gtcactccgc catccgagtg aaagtaaaaa
    11641 acagcatcct tctcaatctc atccattacc tcttcagcgg tcatggtctg gccaaatcct
    11701 ttttcaatct gcttgctttg tgattcggga atcgaacacc aggcacagcg taatgggcag
    11761 ccctttaaat aaacaacggt ccgtaaacca gcaccatcat gtagagaacc ctgttctatt
    11821 cttaatactg ttccttccat atagattttt gaattacaca ctagtttaaa ctaaaacaca
    11881 caaggaaaaa cttaccattt aatcataaaa ttagctcata taaatatact aggatgtaaa
    11941 atagactggt caaaatctgg tcaaaaaaga ggactaatac aggtagtcct cttttttgtt
    12001 attttaatat tctaaatcat tttttattgg tttattccct tattcgatgt agactcaaat
    12061 cactatatca ctagatatca aacggcgtca tacggaatca aaaacatcaa atttgacctg
    12121 ttaaatggtc attgaataac attcaaaatc aataaaacgg agtcagacac ggagtcaaaa
    12181 acgctgagag taaaaaatat atacagcctt gtaggtaaag taccacaggg ctgtatattt
    12241 tttatttcag attaccttaa atgattccat ttacttttta tctcattttt tcctactagt
    12301 tagtatcaaa tactctccta ttttctcaat aaactatttt tggatacatg gtatctgaaa
    12361 cagctaaaac cttgtcatta cagcaaaaaa tcacgttat
//
LOCUS       DAZKOR010000087        12175 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000087 DAZKOR010000000
VERSION     DAZKOR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.088"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..301)
                     /locus_tag="ACG8ZM_002255"
     CDS             complement(137..301)
                     /locus_tag="ACG8ZM_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493186.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            596..1924
                     /locus_tag="ACG8ZM_002256"
     CDS             596..1924
                     /locus_tag="ACG8ZM_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0493187.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2126..2959
                     /locus_tag="ACG8ZM_002257"
     CDS             2126..2959
                     /locus_tag="ACG8ZM_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0493188.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2975..3712
                     /locus_tag="ACG8ZM_002258"
     CDS             2975..3712
                     /locus_tag="ACG8ZM_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0493189.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3867..4835)
                     /locus_tag="ACG8ZM_002259"
     CDS             complement(3867..4835)
                     /locus_tag="ACG8ZM_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0493190.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5067..5900
                     /locus_tag="ACG8ZM_002260"
     CDS             5067..5900
                     /locus_tag="ACG8ZM_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0493191.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5934..6773
                     /locus_tag="ACG8ZM_002261"
     CDS             5934..6773
                     /locus_tag="ACG8ZM_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0493192.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6799..7359
                     /locus_tag="ACG8ZM_002262"
     CDS             6799..7359
                     /locus_tag="ACG8ZM_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0493193.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7558..9279
                     /locus_tag="ACG8ZM_002263"
     CDS             7558..9279
                     /locus_tag="ACG8ZM_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0493194.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9444..10586
                     /locus_tag="ACG8ZM_002264"
     CDS             9444..10586
                     /locus_tag="ACG8ZM_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0493195.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10602..11813
                     /gene="thiI"
                     /locus_tag="ACG8ZM_002265"
     CDS             10602..11813
                     /gene="thiI"
                     /locus_tag="ACG8ZM_002265"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0493196.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4151 a         2042 c         2527 g         3455 t
ORIGIN      
        1 aaataaagct tatgcaaaga aaaagaaatc caatcacttg ggtttctttt tcttttatta
       61 ttatgatgaa tcaagctttc tgtttttgtc acgtatgttg taatctttac gaaagagtag
      121 agttggctac tcataatcaa ccatacttcc catcatcgct aaatacgaaa taaatcagca
      181 ttcctacacc aatcaaagca caagctgcac caagaaaagg gatgacattc ccaaagttat
      241 ctgtattcat aagtatgcca ccacccacaa acaaaaagcc gccagcaatc aggtttttca
      301 ttttattcat ctcctttctt acgttatagt atttccttct ttcaatgagt attttgaaag
      361 ctatcttatt gtatgatgtt cttctaggcc tcttctttat tatacaggcc attttcgtta
      421 tctcatagaa atattccttt ttatctgaaa actattgtat accgtatgac atttttcttc
      481 atgaagttgt aagtatattc tcattcaaat gaaaatttca tcatagaaga gattgtattt
      541 ctaaattctt aagagtaaaa tacgaagtat aacttggatt tggaaggatg attggatgaa
      601 agctattacg cctgatatga caacaaaatt taacaaacag tttcaagcaa atatcaaaca
      661 gcaggcattg cagcgggcag ttgtgaagaa cggaattact gcttctgctg aaaatgtttc
      721 agcacatgtg gagaatacac cggtattttc tatagatttg gctacgggga aagtggctaa
      781 tcaaatgcaa tcggggcgtt gctggatgtt tgctgcactg aacacattcc gtcataagat
      841 gctgaatcaa ttccatttaa aagagtttga attatcacaa aattacactt ttttctggga
      901 taaatatgaa aaggcaaatt atttctatga aaacatcatt gcaacaggaa aagaggcaat
      961 cgatagccgg aaagtagctt tcttactcgc tactcctcaa caagatggcg gccaatggga
     1021 tatgatcgtt tcattattcc aaaaatatgg cgttgtgcct aaatcggtga tgccggaaag
     1081 tgcgaatagt tcaaattctc gagatttgaa taactacttg aacaaattac tgcgtaaaga
     1141 tgcagtagtg ttacggaaaa tggttgctca aggagaaaca ttagacgcta ttgaagaaaa
     1201 gaaagaagaa atgctagaaa gcatttataa cttcttagct atctcattag gaacacctcc
     1261 aaaagaattc gattttgagt accgcgatga agaaaagaac tatcatcttg accgaggact
     1321 gacaccacaa attttttatg ataaatacat cggtgtaaaa ttagatgatt atgttagcgt
     1381 gatcaatgca ccaacaaaag acaaaccatt caatcgttct tatacagtag agatgctagg
     1441 aaatgtcgtt ggcgggaaag aagtcaaata tctgaatgtt gatatggaaa ctttcaaaaa
     1501 attagcgatt gctcagcttg aagaaggaga atctgtttgg tttggttgtg atgtaggtca
     1561 atcttctact agaactagtg gcatcatggc cttagatgct tattcaatgg atgatctatt
     1621 tgatacggat tttacaatga ctaaagctga acgtttagat tttggagaaa gtctgatgac
     1681 ccatgcgatg gtattgaccg gtgttgactt agtagacggg caatcaacaa aatggaaagt
     1741 cgaaaatagc tggggcgaaa aagttggaaa gaatggcttc tttgttatga gtgatgcctg
     1801 gatggacgaa tatacgtacc aaatcgttgt aagaaaagat ttattgacga aagagcaatt
     1861 aaatgcgttc aatgaagaac caattgttct atctccgtgg gatccaatgg gtgctttagc
     1921 atagacgagt atttcaatga agagtatgta gtaaagataa aaagagctgg cttaagccag
     1981 ctctttttat ctttaaagtc cattccctgt tttttatggt ccctgtgcta taattctaaa
     2041 taaaggataa ggattggaaa taatcctttt atcattaaaa atatgatgac gaagtagaat
     2101 aaaattggga gtagggggga aagtcatgga tatccaaact gttttatctg atctgaaatt
     2161 aaatggtaaa gtgattccag ttgttggtgg cgatgttaat cagacctatc gtataaaaac
     2221 cgaacatcgt gcttattttt taaaaatcca tccgaatgta aaaaaaggat tttttgaagc
     2281 agaagtagac ggattaaaag aactgtcagc attcgtacgt gtaccggata cgtatatgct
     2341 aggtgaaaca agtgaaggcg cttatttgtt aatggaatgg atcgagccgg gaaaagggga
     2401 tcaacgagat cttgctgcag ctttagctaa tctccatcag caaacagcgc cacaatttgg
     2461 ttttcgtaaa gataactatt tgggtacact tgttcagaaa aattcttttg aagaagattg
     2521 gtggactttt ttctttaaag atcggcttga atcccagatt tctttggcag aagaaacgaa
     2581 tcgatggaat gtacagcgtc aagaaaagta tctgcgattt aaagaacgtg tattgaaatc
     2641 ggttgaacca aaaaagatca ctccacgcct cttgcacgga gacctgtgga gtggtaatgt
     2701 gttctttgac cagcaagggc atccagtttt tgtcgaccca gctgtttctt atgggaatcg
     2761 ggaacaagat attgcaatga gccagttatt cggtggtttc cgaccggaat ttttagatgc
     2821 ttatcaaact atatttccat tagaaaaggg atggaaagac cgactgccga tttaccagtt
     2881 atattatctt ttggcacatt tgaatatgtt tggagaaagt tacggttccc aagtagatca
     2941 attactagaa aatttttaag aggagggcgt gtttttggca aaaaaatata ccaaaaagca
     3001 tgaagtttca tattatgagt gtgacatcaa ccaaacgatg acatttcctt cgatgttagg
     3061 agtagttatc aaaacgtctg aagatcaaag cgaccagttg ggaagaggca gtgaattcgt
     3121 caatagcttt ggacttactt gggtcatcac gaattattcc atggagatta atcgtctccc
     3181 tagagtcggt gaagtaatct cagtaacgac tcaagccatg gagtacaata agtttttctg
     3241 ttatcggaac ttctggatcc atgatgaaga tgggaaagaa ttagtcaaaa tagaatctgt
     3301 tttcgtattg atggattttg tgaatcgaaa aatgagcagt gttaatgaag agatcattgc
     3361 tccttttgag agtgagaaga tcaagaaaat caagcgtcag gaaaaaattg aaaagattga
     3421 ttcgggcttc atgttgccat atcgtgttcg tttttatgat atcgatagta atcagcatgt
     3481 taataatgcg atgtatttta attggatcat tgacgtatta ggatatgact ttttgactac
     3541 gcatgttcca gaaaaagtaa ttattcgatt tgataaagaa gtagagtacg ggaatgagat
     3601 cgaaagccat tttgaacaaa tccctatcga aaatggtgtc aaaacaaaac atgaaatccg
     3661 aatgcaagaa cagctgtatt gtgaagcaaa tattatatgg aaaaataaat aaaaaagagg
     3721 ttgtgacaga agggggcaac ttcaagaaat aagaagaaat ttacgaaaat tgtttctcaa
     3781 atttttgtaa attttcgact tagttttcga agaagctatt tttgtcacag cctctctttt
     3841 taagtttagc tattagataa tgatgtttag ttttggataa acgaaatcaa ttcttcaccc
     3901 gacaaaccat agtttacagc aatcaatagt tttaaacgtg ctttttgact attaatactg
     3961 ttacagaaaa taatccccat tttttctagt tcttttcccc cgccttcata atcataaaca
     4021 ggctctgcga ttccattgaa acaacgagaa acaagtacta cagggatttt ttggacaaga
     4081 agcttttcca aagcagctaa ggcttgtgga gggagattcc ccgcacctaa agcttcgatt
     4141 accaaaccgt ctactttcgt atgggcgatg gcttccaata agtccccttg catcccagca
     4201 tatgctttaa cgatcgggat agtcccatct acagcggaaa tgtccagtcg ctttgtttct
     4261 aaaagttcct gcaagaataa aattcgattc ttagttacta agccgatggg acccaatgta
     4321 ggtgttcgga aagtagcaac atttgtggta tgggtctttg tcacgtagcg tgcagaatgg
     4381 atctcatcgt tcatgaccac caaaaccccc ttatctagcg cttcttcaca acttgcgaca
     4441 cgaatcgcac tttcaaaatt ataaaggccg tcacttccaa gttcattact tgaacgcatt
     4501 gccccagtca aaacaattgg taatttgttg ccaatcgttg tatcaagaaa aaaagcagtt
     4561 tcttctaatg tatctgtacc gtgtgtgatc accactccgg atatacctga tgcataagcg
     4621 gacaagatac gttcttttaa taccaacatg tgagaaggca tgatgtgtgg tgagggaaga
     4681 ttgaagatat cttcaactaa taattctaca tgcttgggga ttgtgatttc tgcatctaat
     4741 aatgggtttg ccacatcagg actaacaccg cccgtgagca tgttttcttt catggcgatc
     4801 gttccgccgg tgtgtagtac aagtattttt ttcattttac agacttcctt acgaataatt
     4861 ttgcatagcc gttaaatctt attgtatact atataaaaga gttttgctat cagtgaagaa
     4921 taacgaatag cctaacttat cataaaaaag ctgaccttat taaaatggaa aagactcaac
     4981 cttgacctcc taatacaatg gagtatctgg ttgagtttct tttacaagaa agcagccaag
     5041 gaaatcaatc acgaaggtgg ccggaaatga tcagaatcgg attaacatcg tttaaagaac
     5101 atgacaaatt aactggtaaa aagaataaca ctctttatga atatgcaggg tttctaccct
     5161 tagtggaatt agatactgcc tattatggaa ttccccgaaa gtcctcagtt gagaattgga
     5221 taagtgaagt acctgaatca tttcgctttg tagtcaaagt ctatcaagga atcagcggtc
     5281 aaggagactg gaagccttat tatcagaccg aagaagagat gattgcgcat tttttagatg
     5341 ttatgtcgcc aatggtagat gctggaaaga tgttttgctt tttagtccaa ttcccctcat
     5401 cattcaaatg tacgaaagaa aatgtccagt acttgcgcaa acttagaaag tggtttgagg
     5461 gttatcctgt ttctatagaa ttgcgggatt attcttggta cagcgatgaa tttcttgaac
     5521 gtacaagaaa ctttatgtct agagccgctt tttctttagc catcatcgat gagccacagt
     5581 tgctgaatac aacgattcct tttgatccat ttgtcaccaa tccgaacttt gctctttttc
     5641 gattccatgg taggaatgcg caaggatggc aagcaaatga taaagagtgg cgtaaaaaaa
     5701 gaacgttata tcgttattca gaaaaagaac tgcaagaact agcacatgaa gtcaaagtag
     5761 ttgattctaa gacaaaagag gtaggtgtga tttttaataa taattctggt ggtgatgcgg
     5821 ctgaaaatgc attagcactg caaaaaatat taaatatcaa gccagatgga ctcaatccaa
     5881 cccaaatgga tctgttttaa gggcttattt tattcaagaa aggggcgaag tctatgatca
     5941 aagctatttt ttttgatatt gacggaacat tggtcaccaa tcgttcaaaa gcgttggaat
     6001 caacaaaaca agcaattgaa tatgctcgga aaaacggcat tttatgcggt gtagcaacgg
     6061 ggcgcagccc agtaaagatc aaagaaatca ttgatgaatt ggaattagat atgtatgtcg
     6121 tgtataatgg tcaactggtt tttactgcag atcgcacgat tatcgaccac ccttttgaac
     6181 aaaaagtatt ggaacatatt gttgaatttg cagatgagaa ccatcgacaa atcgtttttg
     6241 gtgcgagaaa tcggttagat ggatcaacga cgatgctatt aggacaatcg atttttatca
     6301 agcgactagt cagcttttta ccgcgtaaat tcccggtacg tctgatgaaa aaaatcctcc
     6361 aggtttttag ccctcaccga caaaaggatc gttatgaaac gttagagata ttaaaagaac
     6421 caatttatca atgtatattg ctcagtccgg agtcagaaca gaaaaagtta gaacatcgct
     6481 ttcccgagtg tacgtttcag cgttcaaata cgtatactgt agatatcatc ccaaaaggtg
     6541 gttcaaaatt actaggcatc caagctttcg caaatgctgt cgaaattgaa atggaagaaa
     6601 ttatggcatt cggcgatcat tacaatgata tcgagatgct gaaaggtgta ggaattggag
     6661 tggccatggg gaatgctcag atcgaagtaa aacatgctgc agactttgtg actcaatcaa
     6721 atgaagaaga tgggatatac gaaggactga aacacttcgg tctgatttat tagtcaatga
     6781 aagaaggaaa aaatcagtat ggaagcagag acaccttttc aaaaagtaga aacattccac
     6841 aaaacttttg atccaagaag accagagata cccacggctt ttacagaaaa tgaagctcat
     6901 acacgtgctg cgttcaaaat agaagaactt gttgaatttg ttgcagctac ttcacctaat
     6961 aaagaacaac tgcatgaagc tgttatgctg ctgcacgaag cattagataa agcagaagaa
     7021 aaaatacttg gtaaaacaca gtggggaagt gatccgctgg ttgaacaagt agatgcactg
     7081 acagatatcc tttactttac ttatggttct ttttcattac taggaatagt gcctgatcga
     7141 atattcgaaa tcgtccatca ggcaaatatg gggaaattat ttccagatgg aaaacctcat
     7201 tatgaccctg taacaaataa agtgttgaaa cctgaaaact ggcagatgga atatgctcca
     7261 gaacctaaaa tcgcggcaga aatccaacgt cagattcaag aagcgattgc taagaagaaa
     7321 tccgctgata taggcatgtg cacaaatgaa aaagagtgat cagagtttca ctatttttgt
     7381 tatctttata cgatggaaaa acaaaagatt ttcgtttaag ttagtgaatg catgaaaata
     7441 gtatgctata atctagaata gatgtctttt gcacgagaga cgtattattt ggatttatgc
     7501 caagcagaac gccatctgtt gcataaaaga tgcatactga aaacggagga accataaatg
     7561 ggaaataatg ttatcatcgg aatcgtcatt gcaatcatta tagtcgccat tgtcgtcttt
     7621 ttggttagtt acttgatgaa gaaaaaaaac caagatcgat tggatgcctt agaaaaacgc
     7681 aaggaagaat tatttgactt gccagtgatt gaagaagtcg atgatgttaa aaaaatgcat
     7741 ctggttgggc aaagccaaaa tacctttcgt gaatggagtc aaaaatggaa tgacatatct
     7801 acttcctcgt tcgctgaact agaaagtcaa attttcgaag tggaaaacct caatgaaagt
     7861 tttcggttct taaaaggaaa aaaagcagtt gttgaagcag aagctacaat ggaccaaatg
     7921 gagaaacaag tgacggaaat ccgtgaaggt ctaaaggaat tgcgggaaac ggaagaacgc
     7981 aactcactag ctgttcagca ggcacttgac gtctatgaag aactgaaaga aaaagtcaga
     8041 gaagacgagc atgaatacgg accagctctt tttgaattgc aaaaacagat caaagtgatc
     8101 gaaaaagaat ttacgcagtt tgtgacttta aacacttctg gagaccctgt tgaagcccgc
     8161 gaggttttgg ataaagcaga aaaacatacg tatgaagtgg aagctctgat gaaaaaaatt
     8221 ccaccgctgt atgaggagct ccataccact ttccctgaac aacttgaaga aatcagtgat
     8281 acttacgaga aattgatgga agaacaatat gtattcccag aagaagatct tgctgaagat
     8341 atcgcaaaag taagtcgaag aattgaaaat tcattagcca atcttgaaaa aacagaagtt
     8401 gaaacggtcg aatttgaaaa ccgtgaaaca gctgatttga ttgattcgtt atatgatatc
     8461 ttagaacggg aaatggaagc tcagagatat gtaaaaacta accaatcaac gattgcagag
     8521 tatatcaaac atacaacaaa gaataaccgt caattattga ttgagctgga tcacacatct
     8581 caaagctata cattgaacca caatgaaatt ggccgtgtac gaggtttcca aaccgaagtg
     8641 gaagaaatgg aacgtcaaaa tgaacagatg attccacaga tccgtcaaca cgaaattcct
     8701 tattcagaag tgcgcacgtt ctataaaaca gtcttcaaag tcttagaaga tatcgaaaag
     8761 caacaagtag agatcgacga ctcattacat gaattgagaa aaggcgaaaa agaagcgcaa
     8821 gaaaagatcg atacattcga attcaaattg cgcagtttga aacgttttgt agaaaaacaa
     8881 cgtttgcctg gcttgccgaa tgattaccta gaattctttt ttgttgctac tgaccgtatt
     8941 gaagaactaa gtgttgtctt gaataaaatt cgagtaaaca tggaagaggt caatcgttta
     9001 gttgctctat gtgaagaaga tctagaatta ttagataaga agacacatga tttagtagat
     9061 gcagcagcat taacggagca aatgctccaa tatgcaaatc gttatcgtca tacacacgaa
     9121 gaagtgagag aagcaattga taaaagctac tatttattca ataaagaata tcactatcaa
     9181 gaagcattag acgaaatcgg tacagcatta gagcgagtag aacctggtgc tttcaaacgc
     9241 atcgaagatt tttattttaa tcatccagac ttagtataaa gcaaaaaagt tagtaaacca
     9301 gcaagagcaa gacggattat tgtcttgttc ttgtcttttt ttgtatattt gaaaattgaa
     9361 acaagtgaaa atttcacctg ctcttgaaag caccttggac aaccgttata atagaaaaga
     9421 atttcaaaaa agaggggttt cttatgatat attttgataa cagtgcaaca accccaatct
     9481 ttccgcaaag cttagatgca tatgtaaaaa ccagtcagcg gattattggg aatccatcaa
     9541 gcttgcatga tttaggcaat caagctaacc ggttgttgca gcaagcaaga aagcagatcg
     9601 ctgatttgat ccatgtagaa ccagaagaaa tctattttac ttcaggcgga acagaaggag
     9661 ataactgggt attaaaagga acgatgatcg aaaaaagaga atatgggaat catatgatca
     9721 tttcaggagt cgaacatccg gccgtcagcg agacagcaga acaattaaaa gaattaggaa
     9781 tcgaagtaag tatcgcccca gtggataaac gcggatttgt acgtgtcgac gaattgaaag
     9841 aattaataag aaaagagaca gtattagtct ctgttatggc tgtgaataat gaagtaggat
     9901 cgattcagcc catccaggaa atcagtgatc ttttggatga ttttcctaaa attcatttcc
     9961 atgtagatgc tgttcaggcg attggaaaag taccattctc gcaatggttg acagaaagag
    10021 ttgattttgc aacattttca gctcataaat tccatggccc acggggaact ggtttcattt
    10081 attggaaaaa gggtcgtcgt ttagcaccat tactgacagg tggagggcaa gaaagaaatc
    10141 agcgaagtgg gacagaaaat gttccaggta ttgtggctat ggttaaagct ttacgtttat
    10201 cattagagat aaaggaacaa aaacctgagc agacacaacg cttaaaagta gctctaatca
    10261 atgcgttgaa gacgtatgag aaagtgacga ttttttcaga aggaactgac tatgcaccgc
    10321 atattttatg ttttgctcta aaaggaattc gcggcgaagt tctcgtccat gccttagaag
    10381 aaaaacaaat atttgtttct actactagtg cctgctcctc acgtaaacat atggcaagca
    10441 gtacattgca cgcaatgcat gtaccaaatg atctggctac atctgctgtt cgaatcagct
    10501 tagatgaaaa caattcgctt gcagaagtcg agcaatttat gattattttc aagcagcttt
    10561 ataagaaatt ttctgcaatc aattaaaaga ggagtgttct tgtgaaatac agtgaaatta
    10621 tggtccgtta tggggaactt tcgacaaaag gaaagaatcg aaaaagcttt atcatgcaac
    10681 tagcacaaaa tgtcagacaa gctttgaaag actttccaga aatcaaaatc catgctgatc
    10741 gggaccggat gcatctttta ttgaatggcg cagacgggca gctagttatt ccaaaactag
    10801 caaaaatatt tgggatccaa aatttctctc caagtattcg tgtagaaaaa gatgtcgaag
    10861 ttttgaaaca agctgttcaa gacatcatga aagaaattta tactgggaaa gaaacattta
    10921 aaattatcgc taaaagaagc gatcaccaat ttgaattgac aagtaatgaa ttgaatcaaa
    10981 cattaggtaa tgctgttttt gatattttcc cgcatatcca agttcaaatg cgtcaaccag
    11041 atattccatt acgtgtagag attcggcgtg atggcgctta tctatcgtat gaaacgatca
    11101 agggagctgg aggattgccc gtaggaacaa gcggaaaagg aatgcttatg ctttctgggg
    11161 ggattgattc tcctgtagca gggtactttg ccatgaagcg gggagtggaa attgaagctg
    11221 ttcattttgc tagtccgccg tatacaagtg aacaagcact gcaaaaagca aaagatctaa
    11281 cagccaaact agcaccttat gtgggaacga tccattttat cgaagtgcca tttaccgaga
    11341 ttcaagaaga aatcaaaaag tcagtgccac aaggatattg gatgacaatt actcgtcgaa
    11401 tgatgttgcg tttgaccgat gaaattcgcc gtattcgtca tggtttagtc attttgaatg
    11461 gtgaatcatt aggtcaagtg gcttcacaaa ctttgcaaag tatggtggct atcaatgaag
    11521 taacgaatac gccaatcatt cggcctgtag cgacaatgga taaacttgaa atcattgaag
    11581 tagcagaaca gatcgataca tttaatcttt ctatccagcc ttttgaagat tgctgtacga
    11641 tttttgctcc tccacagcca aaaacacgcc ctcaactgga aaaggtccat caatatgaag
    11701 agcgtttagc aatagatgaa atgatccaac gagcactagc tggcttgaag atggaagaga
    11761 tacaagcaga atcgtctaat gaaaaaactg aagaattttc agaattgcta taagaggtta
    11821 tgaaaatagt gttttgacca gagtattaga gaaacaaaac gaaaaaatag ctccatgtat
    11881 tttaccgttt ttgaactaat gtcgaagatt agctgattga ataccattta tcaagaagtt
    11941 atggaaaagc cggttctttt ttactaactg aaaaataaaa ttttatttag agatcaagct
    12001 acagttgata aatgatataa aaaagagaat ttcccatgaa atagcgaggt tgtgacagaa
    12061 gtgggcagct tcaagaaata agaaggaatt tacgaaaatt gcttttcaaa tttttgtgaa
    12121 atttcggctt atttttcgaa gaagttactt ctggaacacc gtgtatcaga ggttg
//
LOCUS       DAZKOR010000088        12164 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000088 DAZKOR010000000
VERSION     DAZKOR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.089"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..731
                     /locus_tag="ACG8ZM_002266"
     CDS             159..731
                     /locus_tag="ACG8ZM_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0493197.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            876..1580
                     /locus_tag="ACG8ZM_002267"
     CDS             876..1580
                     /locus_tag="ACG8ZM_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0493198.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            1808..2146
                     /locus_tag="ACG8ZM_002268"
     CDS             1808..2146
                     /locus_tag="ACG8ZM_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493199.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2131..2985
                     /gene="lgt"
                     /locus_tag="ACG8ZM_002269"
     CDS             2131..2985
                     /gene="lgt"
                     /locus_tag="ACG8ZM_002269"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0493200.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3132..3365
                     /locus_tag="ACG8ZM_002270"
     CDS             3132..3365
                     /locus_tag="ACG8ZM_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0493201.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3563..5467
                     /locus_tag="ACG8ZM_002271"
     CDS             3563..5467
                     /locus_tag="ACG8ZM_002271"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0493202.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5777..6139
                     /locus_tag="ACG8ZM_002272"
     CDS             5777..6139
                     /locus_tag="ACG8ZM_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFQ0493203.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6156..6767
                     /locus_tag="ACG8ZM_002273"
     CDS             6156..6767
                     /locus_tag="ACG8ZM_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFQ0493204.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6869..7777)
                     /locus_tag="ACG8ZM_002274"
     CDS             complement(6869..7777)
                     /locus_tag="ACG8ZM_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0493205.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7819..8133)
                     /locus_tag="ACG8ZM_002275"
     CDS             complement(7819..8133)
                     /locus_tag="ACG8ZM_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0493206.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8432..10363
                     /locus_tag="ACG8ZM_002276"
     CDS             8432..10363
                     /locus_tag="ACG8ZM_002276"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0493207.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10656..11207
                     /locus_tag="ACG8ZM_002277"
     CDS             10656..11207
                     /locus_tag="ACG8ZM_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0493208.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11520..12062
                     /locus_tag="ACG8ZM_002278"
     CDS             11520..12062
                     /locus_tag="ACG8ZM_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR4 domain-containing protein"
                     /protein_id="HFQ0493209.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         3952 a         2034 c         2556 g         3622 t
ORIGIN      
        1 gaatttaata gagaagcctg gcgtctatta agaaataatt ttcttgatga gcgtcaattt
       61 ttttaacgga aatctatcct atactaaatt cagtaaagaa aaaggaggaa tcaaagaaag
      121 ctattttaaa caaaccaaaa ttataggagg aataaatcat gactgaaaat aaagctactc
      181 aagaaagaac accccaagaa agattgcaag aagaacaaga acacaaggaa catgttcatc
      241 atacgaaaat taatgcagcg gccattgcgg atcatctttt aggcaacatg catacattac
      301 atgtgaaact gcaccaatat cactggtatg taaaaggagc caatttcttt acgttgcatg
      361 aaaaatttga agagttgtat aacgaaaatg aaaaatggtt cgacaaactt gcggagcgtc
      421 taattacttc tgggcataaa cctgcttcga caacggttga atttgaaaaa tattcgatgc
      481 tttcggaaga tgcagttaat aaatacgcta aagcagaaga catggttgaa aatattatcg
      541 aggacttcag aagcactcgt gacctaacta ttcgcgcgat tcgtttagct caagacgagg
      601 gtgacgatgc tttagaagat acattgattg cttttaaaaa tgacttagac aagaacattt
      661 ggatgctgca agcgttcatt ggtaaagaag cattagaaga tgatgacgct cttgatgagg
      721 atgaagatta aggtaaaagt agaagctgat ttagggattg gaattctttc cagtcctttt
      781 tccattttct tgatgtgcat caattttttt gtagtaagag acttatataa taaaagcagc
      841 gaatataaac aaaataaata aagggggaga atgaattgac taaacatacc catcatgaaa
      901 ttggcgacca cgcagcctgt atccgcttgg ttccaatatt caatcactta gaggatagcg
      961 cgatgaatta cattgctgaa aaagcccaca cgaaacagta tcaaaaagga gagtttctgt
     1021 ttcgttcaca agaaaaagag gatactttat atattatcaa tcgtgggaaa atccgtattt
     1081 atcgattggc agactctggc aaagagcagc tagtgcggat cttgaaccct ggtgatttca
     1141 ccggagaatg gaccttgttc aacccggatg cagtacacga ggaatatgcc gaagcaaccc
     1201 gagataccgt tgtctgtatg attcagcaaa aagatgtcca agattttctg gaacaatacc
     1261 cagctatttc tttaaaacta ttggcggaaa tgtctaaacg gttagaaagt tcagaacgtc
     1321 aaacgacaca agtagccgtg gagagtgtca ttacccgttt agttttgttt ttggcagaaa
     1381 atgtggaacc tgaaatgggc aactctccca ccatcaccct gccgatggca aaaaaggaca
     1441 ttgcttccta tttaggaacg acacctgaga ccatcagtcg caaatttgga gaattggagg
     1501 acgaaggatt gattcaacag ctgtctggga aaaggatcaa gattcaggat ttagatgatt
     1561 tattgcttta cagcgaataa tcggacacac ttttttctac acgttggtgt tctgtcgctc
     1621 ttcattaaca acgggacaga actcagtgct tttgcttcca tctgtccgtt tttggctttt
     1681 ttaagacaga acgaccttca ttcattggaa aaataaaagc acaaacatga taaccctcaa
     1741 gtttttttgt tttatttttc tttaagatag ggttgtcgaa caaaacgtaa aaataagtag
     1801 ggtgaatgtg cattgttttt gggtgatagt tgccatcacg ttgtcagcag cgggggcatt
     1861 ctttgtcgtc cctttcacca agaacacgat tggcggcgaa gcaaaaacgt ctaacgttgt
     1921 tgaggcagat aggttagcta tggaaaaaga agaggaagtt gtaaaaggag ccggcgtaat
     1981 cggacacgga aaagtggcgt attacgaagc gagtgaccag ggagataaaa gtgtattcac
     2041 tgtttggttt ccatggagct tagccggaat cttgttcagc atcaccatga ctagaatgac
     2101 gcgaaaaatt gaagaaatgg agcaaataga atgggattcg tattaggcgt tttgaatccc
     2161 catgtggtgg agattggttc tgttgtgatt cattggtatg gagtcattat tgcagcgggc
     2221 atattagcgg ctctccagtt gagcaccaag gaagcaaaga aaaaaggact ggaagaggat
     2281 accattatcg atatggcctt atgggcaatt ccgattggac tcctcggcgc tcggctttat
     2341 tatgtgttat ttgaacttgg ttattatctg caaaatccag gacaaatcct tgcgatttgg
     2401 aatggcggca ttgcgattta tgggggattg attgcaggag ggggtacgct atattggtat
     2461 gcaaagaaaa aaggtacttc tttggcactc gttcttgata ttttagcgcc caatgtattg
     2521 ttagcccagt ccattggtcg ttggggcaat tttatgaatc aggaagctca tggtgggccg
     2581 gttactcgtc aattcttgga aaatctttat ttacctgaat ttatcattga acaaatgaat
     2641 atgaatggta cctactacca tccgactttt ttatacgagt cattgtggag tttgttgggc
     2701 tttgtcctca tcgtcactat acggaaccga aaacagcttt tgcgtcaagg agaagtcgct
     2761 ttgagttacg ttttgtggta ctcagtgggt cggttcttca ttgaaggtat gcgaacggac
     2821 agtttatgga ttggcgagtg gatccgcgtt tcgcaagcct tgtctctggc cctgtttatt
     2881 ggcgcgattg tagtatggtt tatacgcaga caagattatc caccaatttc ttattattct
     2941 gatggcaata aggggcagaa aaagactatt aagatggtga attgagcaaa aagataaaaa
     3001 gaagtgcaaa acttgatatg cgtcaatttt cgtttgctaa aagtgagcta tactacagtt
     3061 gtaaagagga aaaggcagtg ttcattaatc tgctgccgat aaaataaaaa cacataaagg
     3121 agataggaat tatggaaaaa gctatattgc aattagaaac gttgtcttgt ccaagttgta
     3181 tgcaaaaaat tgaaggtgcc gtgaaatcag ttaacggtat tgataaagac agcattaaag
     3241 tattattcaa ctccagcaaa gtcaaaacca attttgattc agctgtcact tcgattgaag
     3301 agattcaaaa agctatcgaa aacgtaggct atccggttct taaagcaaaa gtcaaggccg
     3361 tttaacagat taaatgaact aggcaattct ggaagaaaag ctatttttcc aggattgctt
     3421 tttttgttgc aatccgattc gaggtgctta gaaacgataa aagagaactt tctaaacttg
     3481 atgtcagtca attaattact taggcaatta tcttatactg tgtttatcaa taaaagagaa
     3541 attaaatttg gagggaaaaa gcatgcaaca atatatatta agcaagaaaa atgttatcac
     3601 cgtcatcagt ggattgttaa tcgtactcgg tttcttcagt cactttgttt tagaaaacgt
     3661 aggactttca gagtggtctt tgatcatcgc ctctgtcttt gggattacgc caattgccat
     3721 tcaagcgttt caagcaatga aagtcaaagt catcagtatt gatgtcttag tcagtattgc
     3781 agcgattggt gcgcttttta ttcaaaatta tgaagaatcg gctattgtca cgttcttatt
     3841 cttattcgga cactacttgg aacaacgaac attgaaccaa acgcgatctg ctatcaaaga
     3901 attgactgaa atggcacccg aaagcgcctt gaaacaaatg gataatggcg aatttgaaga
     3961 agtagaagtg gatgatatag atgaagggga tatcttgctc gttaaaacgg gtgcgaaagt
     4021 tccagtagat ggcatagtcc ttacgggtga agggcatatc aatgaagcta gcattacagg
     4081 tgaagctatt ccggtcaaca agctagctga tgcagaagtt tttgcaggaa ccattttaga
     4141 aaacggaacg attcaaatca gagctgaccg agttggtgag gacaccacat ttggaaaaat
     4201 tattgaactc gtggaagaag cacaagattc taaatccgaa gcggaacggt tcattgatcg
     4261 cttttctaaa tactacacac cagctgtctt ggttctggca attgttgtat gggcgttcag
     4321 tcaaaatatt gagttagcaa tcaccatctt ggttctaggt tgcccaggcg cattagttat
     4381 cggagtgcct gtctcaaacg ttgccggtat tgggaatggt gctcgtaacg gtgttctttt
     4441 aaaagggagt gaagtcattc aagacttcag taatgtggat acaattgtat ttgataagac
     4501 aggtacttta actgtcggaa acccaaccgt tgcagcgact gaactgtatg aaaaagatcc
     4561 tgctgaaacg cttggctatc tggccagtgt ggaacgggaa tcagatcatc cattagcaaa
     4621 agcggtctta aatcaaattg gagaaacaaa cttttatcct gttgaaggaa ctgaagttgt
     4681 gaaaggcggc ggaatcgttt caagtgtagc tggacataga gtggctgttg ggaatgtggc
     4741 cttgatggaa aaagaaaatg tcactctcag caaaaaagtg caaaaagatg tcaaacggtt
     4801 tgaacaacaa gggaactctc tcgttttgac agcggtcgac ggtgaattaa aagtactgat
     4861 gggcattcgc gatcaagtcc gtccgggtgt gaaagctaat ttgcaggaat taaaagactt
     4921 gggcgtgaaa aatctagtcg ttctttcagg agataaccaa ggaaccgttg atgtggtcgc
     4981 cggcgaactt ggtttgaccg aagctcacgg ctacatgttg ccggaagaca aatcggctta
     5041 tatcggaaaa cttcaacaac gtggtcaaat tgtagccttt gttggagatg gcgttaacga
     5101 tagtccgtcc ttagctttag cagacattgg aatcgcaatg ggaagcggaa cggacgtagc
     5161 gattgaaaca tccgatgtcg ttttaatgaa ctcgaacttc agcaacttgc ctcacgcatt
     5221 aggattagta aaagccaccg caaataatat gaaacaaaat atcgtgattt caattggagt
     5281 agtgttggtc ttgttgacca gcgtcttctt cagcgaatgg atgaatatgt ctatcggaat
     5341 gttggttcat gaaggtagta tcttggtggt aattttcaat ggcatgagat taatgaagta
     5401 taagttgaaa ggtcgaaaag aacaaaaaga agtatcagca cctgcagaga aagtaaaaat
     5461 atcttaagaa taaattagaa gaggctgaga caatagtcca tccttgaaat gacaaaagcg
     5521 gaaactcaga tttttaaatc tgggattttc cgcttttttg agttcttgaa aaaaaataag
     5581 agtatagagt atgttcttta taaaattaat tttcgtgtaa atgattaaaa aatttaataa
     5641 taaaatttaa attcctatta agttgtaaca catgaaattg cttataatag tgtattgaaa
     5701 ttaaggtaga ttgacaaacg agtgatcaaa tagatatact accttcaaac gaccgtttga
     5761 atgtgaggtg agatgaatgg aaaaagtttg ccaagtgact atcgtacata aagaaaaagt
     5821 aaatgtaata aaaaaaaaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     5881 atgtttcagc gattcttcta ggattaaaat tttctatgct ttagaaacct ataaggaaat
     5941 gtgtgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     6001 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     6061 ttctttagcg aatgaagaca tcctttccgc tattcgtgtt tttttaaagt tatcagaaaa
     6121 tctatctatg aaatcttagt ttggaggaag ttattttgtt acaaagtatt ttttcagcta
     6181 tcgctgttta tatttctacc agtattgatt atttgtttat cttgctgatt atcttttctc
     6241 aaagccacac aaaaaaaggt attcgtcaca ttttttgggg tcaatatctt ggaacaggta
     6301 ttttggtagc cgtaagtttg tttgctgctt atgtgctaaa ttttattccg caagattgga
     6361 tcattggtct tctcggtcta attccgattt tcttaggaat cagagtcgct tttgttggcg
     6421 aagaggagga agaagaagag gaagtcgttg aaaaactcga atctagagga actaatcgct
     6481 tattctggac agttgcctta ataacgattg cttctggagg cgataattta ggtatttata
     6541 ttccatactt tacctcgtta gctttttcag aaatcattat tgctttaatc gtattcgcta
     6601 tctctgttgc ggttctctgc tatatcagtt acaaattagc caagatttct tttgtttccg
     6661 aaacgttaga aaaatacgaa cgaatcattg tccctataat gtttattgcg ttaggaattt
     6721 ttattatgat agagaacggg acgatacaaa cgctactagg tttttaaaaa tcataatcac
     6781 gaaagataaa aagagaggca gcgactaatc aaaatcgctg ccttttttgc ttttcaaata
     6841 atgaagtatt ttttagcgta gaactatctt acgtgttgtg ggagtatccc ttttcaagct
     6901 cttctttgct tgtagttatt tctattgtga tatgattctc tacgcttgat ttgtgtgcga
     6961 cttctgcaat ttcttctttt atttccctag ccttgtctat gtctgaaggg tctataagaa
     7021 ccgtgatgat aagggcgttt tcttccccgt ccagtgacca aatgtgaaaa tgtgagaggt
     7081 cttttacagc aggaacattt ttaatggagt ccgccaagct ctttacgttt acattttcag
     7141 gcgaaccatt caataaaatt ttcattgtgg tgaaaaattc aggaacggtt ttgtagagga
     7201 tataaagtgc aatcaaaatc gataagatag gatccagacg atatgcctcg gtaaagttca
     7261 ttagaatact cacaatcaag actccaatcc accctaatac gtcttccagc atatggaggt
     7321 tcaacatgct ctcgtttttg gatgtgcctt tgcttaaaat ccacgcagca tatccattca
     7381 atgcaatagc tacgagagaa agccagaaca tgccactata atttacgggt tgtgggtcaa
     7441 tcaaacgtgg cacactacga tagatcataa aaaacgagcc actaattagc acaacgccag
     7501 ttatcagcgc gcccagtaaa gaaaagcggt tatagccaaa agtgaaccgt tcatcttgtt
     7561 ttttctctga ataaccttga aaaaaccaag cgaatccaat tgaaatagag tcccctaaat
     7621 catgaactgc gtcggacata atggacacac tattgaaaag aaagccaaag aaaaattcgc
     7681 ttatagagaa aataagattt atgaagaaag caattttaat attctttttc ggttgattct
     7741 gattcgagtg attatggtct ttatctgatt ttgacattta aaaacctcct aagtttgtat
     7801 gctacatact aattggtttt atttttctaa ttcattaaca tgagttaaga cttgttcgag
     7861 aatactaaaa acatgaaggt catctaaact gtaaagcata ctttttcctt ctcttcttga
     7921 tttcactaag cgtgcgtctt tcaacgtttt taattgatga gaaatagcgg actgttccat
     7981 gtccagagaa atagcgatat ttccaacact acactctttt ttctgtaaaa gaaagagaac
     8041 cgacaatcgt gtaggatcac taatcacctt aaatagtttg cttattgaca gaatagatcc
     8101 tttatcgatt ggagaagttg tttttatatc catagtgttt ttattccttt catatgacct
     8161 tccgttcata tctatattac catatgagcg aaacaatgct aatcttttgt ttataaaaga
     8221 gaagtggcgt tctctttatc ttgacaaaat gtatatagcg atataaaatg agtatacaaa
     8281 catatgatta attagtcata tgtaaggtgg attcattacg atttactttt ggaataaacg
     8341 aaaggagtca gaaatagcag aaatttatgt gattcaagaa gtaaatgatg gaaaacaaag
     8401 gaattataaa ttatataggg aggcaataga agtggaaact atccaagaac aacagtctca
     8461 agaaaagcat aatcataatc acaaccacga tcacgaccat gatcatggga aaatgccagt
     8521 cgttttgtac tttgttggtt tagcattagc agtgattgct ctatttttga atgaagatta
     8581 tcagttggta caaaatgttt tgttctcaat cgctacaatc agtgccgggt atcacgttat
     8641 tcttcttgaa ggccttggag aaacaattga gaattcaaaa gcacaaaaaa agttcatacc
     8701 taattctcat attttaatgg gattagcggc aattggagct tctttgatgg ggaatttttg
     8761 ggaaggaaca ttattgattc ttattttttc tggcgcacat tttttggaag attatgcaga
     8821 aggaagaagc aaacgagaaa taacgaaatt actcgaaatg aatccaacga ctgcccggtt
     8881 aatcctgcct gatggtagta caaaaaatgt ggatgtcagt gaattaaaag tgggagatca
     8941 acttcaagtg ttgaatggcg atcaagtacc tattgatggc gtcattttgt ctggatctac
     9001 atccattgat gagtcgtcta ttactggaga aagtatcccg aaagagaaat ccaaaggaga
     9061 tggcgtcttc gggagtacaa tcaatggaac aggtaccttc acgatgaaag tcaccaaaga
     9121 aaacaaagat acagtatttt caaaaatttt acaattagtg aatcagaacc aagataacca
     9181 aacaaaagct gcgagtatta tccaaaaatt tgaaccgaaa tatgtcacag ttgttttaat
     9241 tgccattcct ctattcatgt tattggctcc ttttctactt gattggacat ggtcacaaag
     9301 tgtctataga ggattggtcc ttttagttgc agcttcaccg tgtgctttag cagcagctac
     9361 tgtctcggca acgctatcga cgacttctaa tttagccaaa aaaggagtcc tttcaaaagg
     9421 tagttcttac ctgtctcaat tagccgatat taaagcaatt gcatttgata agacgggcac
     9481 cttgaccaaa ggaaaacctg aagtaaccaa ttattatttt gctgattccg tgaacgaaga
     9541 aaacatgatt gatatcgtag tagctcttga aaaagaatcc aatcacccct tagcagatgc
     9601 tattctaaga aagtttgagc aaaaaaacaa actaactatt gaagtagaaa accaaattgg
     9661 taaaggattg acgggagatt acaaagggag aaattatcgt ataggaaaac cgacttcgtt
     9721 tgatgatgtt tcagatgaat atagtcgttt aaataatgaa tgggcgtcag aaggtaagac
     9781 agtcgtatac gtagcagtag atgaagaggt tataggcctt attgccctta tggacgttcc
     9841 gagtgaatat gctaaagaaa ccattgaata ttttaggaaa caaggtatac acaccacatt
     9901 aattactggt gactcagaaa tgacgggaaa agcggttgct aaacaattag gaatagatga
     9961 agtgattgcg aatgtcatgc cggaagacaa atccagaatt atagacgaac aaaaagaaaa
    10021 atacggcgtg accgccatgg ttggagacgg tgtaaatgat gctcctgctc ttgttaatgc
    10081 agatgtggga atcgctatgg gagatgggac tgatgtggca gtagaggtat ctgatttagt
    10141 tttaatgcaa aacaatttat ctaaattggt acaatctcat aatatttctt cgaaaatgaa
    10201 ccgtgttatt tggcagaata ttattttttc aatggccgtt gttgcttttt taattgtcgt
    10261 tagtttctta ggcttaacgg atattgcaat cagtgtcatt attcatgaag gaagtacttt
    10321 ggttgttatt ttgaatggac ttcgattgct aaaggcagtc taaagaatga ttgcacaact
    10381 tcaaattaat cgagtaatag agataattaa ggattacact acatttttta tctctattac
    10441 ttgtttatat ttatctagat gttcaaagtc aaaatgatat atcaaccata ctcttgtggc
    10501 gcgggttgaa tataagatac atgaaattgg ataaaatcat gtatcaagaa tcctatcctt
    10561 atagggttct ttttttttac tccccctaaa cacacgattt cgtgttgtaa taactataat
    10621 catgtattcg aacagactaa aaaggaggga ttgtcatggg ctataatgtt cagccactac
    10681 ggtcgcaaca ggagattaat gattttttat tttgcctgcg acggaataaa aatgcggaac
    10741 gtgacgtttt cttgtttcta attggaatta acagcggtct gcgcatgtcc gacatcgtta
    10801 aactcaagaa aaaagacgtg attacctcaa aaaacccgcg catcgtggag caaaagacag
    10861 ggaaaacacg cattttatac ttaagcagct tacaggactt gattcacggc tataccgcac
    10921 ccttagatcc ggaggcttac ttgtttccca gtactaaggg cggccattta gaagtcaata
    10981 cggtctacca gatgtttcaa aaggtcgcga agctgttagg aagggacgat atcggcaccc
    11041 acaccctacg aaaaaccttt ggttaccact attacaagaa aaccaaagac gtggccacct
    11101 taatggaaat ctttggtcac agcagcgaga agattaccaa acgctacatc ggaattaatg
    11161 aagatgaaat cagtgaaacg ttattaaatt ttcggttagg tttttgattg tattatataa
    11221 tagataagca cagtaagacg gttataatca cccacagtat tggacaaatc aacctttttt
    11281 tggtatatta tatatacaaa tgagagcagg ctcatagtca acttgatata aatccggaac
    11341 ataagggtcc ggcgggtggc gagcaccaaa actaaactcg atacaaatcc ggaacgtaag
    11401 ggtccggcgg gtggcaagca ccaaaactaa acctgtacaa aaggctgctc ttttgagcag
    11461 ctttttttat tgggaaaaag gagcattctt cgcacgcggc aaaaaaagaa tacaaacgca
    11521 tgtctataaa agagacaacc caaattacca gacagttaaa cgcgatttat aaggctgctc
    11581 atctcttaca agaacatttt gtagataaaa aagtatcctt tgtcggagaa gtctcaacgg
    11641 tagcgattat tttttcgaca actaatttta tgcacctctg tggcattgat tatcgcagag
    11701 gcacacactt gttttttcaa gatgccttag acaggaaaat aaatttacag gatattcaaa
    11761 tcaaaacgga tgggacgacc tttcagaaat tacaagtaat aggtagcctt gatttattat
    11821 tggggaagca tatttctatt gttggcagag gtgtttactc ttctcttcgc tatgatgcag
    11881 ctattcgaac aaggaaaaaa atattggctc tgtctttgaa acaaaacgga ttaatctata
    11941 tccctatctc tttgttaaac ctatcatcaa aggaaattgg acctggacaa aaagttactg
    12001 gcatttttag cgaggattta acatccgggg aacttaaaat gattatggaa gtcatcgatt
    12061 aatcattaat agtgatatgc gacgtcattt tgaagtaatt attctatttt gggttataaa
    12121 ataaagcatt ttaagacgat ttcaaatcat tcatgtgggt tatt
//
LOCUS       DAZKOR010000089        11705 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000089 DAZKOR010000000
VERSION     DAZKOR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.091"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..728)
                     /locus_tag="ACG8ZM_002279"
     CDS             complement(114..728)
                     /locus_tag="ACG8ZM_002279"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0493210.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(875..1558)
                     /locus_tag="ACG8ZM_002280"
     CDS             complement(875..1558)
                     /locus_tag="ACG8ZM_002280"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0493211.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1667..2125)
                     /locus_tag="ACG8ZM_002281"
     CDS             complement(1667..2125)
                     /locus_tag="ACG8ZM_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0493212.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2209..3972)
                     /locus_tag="ACG8ZM_002282"
     CDS             complement(2209..3972)
                     /locus_tag="ACG8ZM_002282"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0493213.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4193..5653)
                     /locus_tag="ACG8ZM_002283"
     CDS             complement(4193..5653)
                     /locus_tag="ACG8ZM_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493214.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5861..6787)
                     /gene="glsA"
                     /locus_tag="ACG8ZM_002284"
     CDS             complement(5861..6787)
                     /gene="glsA"
                     /locus_tag="ACG8ZM_002284"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0493215.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6810..8237)
                     /locus_tag="ACG8ZM_002285"
     CDS             complement(6810..8237)
                     /locus_tag="ACG8ZM_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0493216.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8268..9482)
                     /locus_tag="ACG8ZM_002286"
     CDS             complement(8268..9482)
                     /locus_tag="ACG8ZM_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0493217.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9568..9795)
                     /locus_tag="ACG8ZM_002287"
     CDS             complement(9568..9795)
                     /locus_tag="ACG8ZM_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493218.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(9823..10065)
                     /locus_tag="ACG8ZM_002288"
     CDS             complement(9823..10065)
                     /locus_tag="ACG8ZM_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0493219.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10097..10654)
                     /locus_tag="ACG8ZM_002289"
     CDS             complement(10097..10654)
                     /locus_tag="ACG8ZM_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0493220.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10667..10855)
                     /locus_tag="ACG8ZM_002290"
     CDS             complement(10667..10855)
                     /locus_tag="ACG8ZM_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0493221.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10868..11434)
                     /gene="amaP"
                     /locus_tag="ACG8ZM_002291"
     CDS             complement(10868..11434)
                     /gene="amaP"
                     /locus_tag="ACG8ZM_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0493222.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3430 a         2428 c         1916 g         3931 t
ORIGIN      
        1 acttctaagt actgcccttg cagaaaatca ttcactgttt catattcttt tttcatttct
       61 tttgaatctg tcgatggttt atagcttatt tttaacataa ctagtgctcc tttttattcg
      121 ggacgtgcaa tcgtgaaaat ttctcctatt gctgcataat catttcctgc caaacggctt
      181 acagcattca attttcttgg atcgatcctt cctgtcttat catcatagac tgtttcattt
      241 aaatgatatt tctgtacttc tagcaaaaat aaatcagctg ttactttatt tttattccgt
      301 atctcgacat gttcatataa ggatgtttca aaacgaactt ttgcttctgt caatgctggt
      361 actgaaatga ttactgaatc agttaaagtc aagtcagtca ggtacagttc gctttcctca
      421 ttaggcagat tggctgctgt tttatttact tcttctacgt tgtcctcatc aaggatatgg
      481 ataacagctt ctcttgtctc aagaatattc tttgctgtgt cttttactgc atcttgtttt
      541 ctctggatcg ccaaggataa gatgggtcga ttcgaactga cgatattgaa gtaactgaag
      601 ggtgcgatat tcactatatc ttttgctgat ttagtcgtta ctactgctac tggtctaggg
      661 atgactgaac caatcagcaa cttgtaattt tcacgttctg tcaattctga aggactccgt
      721 tcaaacatgg tttcgccttc tttctataaa attcgttaaa aagcagatct tgaagacaaa
      781 atgaaaagta atgctttttt catcttctta gaatctgctt taaactatct atcaattttt
      841 cttttttgtc aagaatctgt gccgtgcgtg ttttttagtt atttgcttta cttgcggctt
      901 cgacatatct tttagtgatc aattgcggac gggtgataat agatccaact acgactgaaa
      961 aagcacctaa ttcatatgct ttctctactt gttcaggaga gtcagtatgt ccttccataa
     1021 ttactgggat atcgacttgt tctatgattt gattcaataa atcaaaatca ggtgtctgcg
     1081 tctttcgtga atattctgta tagcctgcta aagtcgtaga caaaagatca aaaccatctt
     1141 cttgcgcttg aagcgcttcc tcaaaagtcg aaatatctgc catagctaac cgaccgtgtt
     1201 cgtgaatcat ttggacaagt tcagcagttg ttttttctgt cggacgttta cgagaagttg
     1261 catccagtgc aatgatttct gcttgcgtct ccaaaagttc ttccacttct ttttctgtcg
     1321 cagtaataaa cacttctgac ccatcgtaat ctcttttgat aatccctatt accggtaaat
     1381 caacgattcg cttgatttca ttgatatctt ctgtcgtatt tgctcgaata ccaactgctc
     1441 cgccttcttt tgcagccaaa gccattctcc ccataataaa agaactgtgt agcggctcgt
     1501 tttccaaagc ttgacaagaa acgacgactc cgcctttgat ttgttccagt gtatccacaa
     1561 tatctcctcc agtaatgtta tataaaaata cagcttcata taaaaatgta acccttttct
     1621 attcaggaga caataatgag ctatttcttt cagcagacct ttcttactaa aagaaaatta
     1681 cttcaaattc ttcgtatacg accagcttct cttctgtaaa ttctatgttc aacgcttctt
     1741 tataagcatg tttaaaaaac tcagcatgga ctttccgcca ataagctaat gtccgatccc
     1801 cttctccttc tttccaagca tgctctttcg tgacttcact aaaaggcaag atggtcacat
     1861 ttatattttt gatgatacaa actggttcat tttttccatt caggaccacg tctaattgac
     1921 caacttttgg taaaggctca ttctcttgtt tatacagatc gtaaccactt gatgtaccag
     1981 tctttatctt ttttacaact agttcgctca actcgtctgc cattttttct gtatccccaa
     2041 atgaaaagaa acctaatttt tctgattgat aactaggata tttttcttgg aactttttga
     2101 catattcttc tacttgctga tacataacat ccaccccttt gctttttata atgaaaaaac
     2161 catccagata ctagtataaa caattatctg gatggtgagc ataatgaatt attgaatatt
     2221 caagacacgc agactatctt caacgatttg ttttggtgtt aaatgataac gttcataaag
     2281 ctgccctaca ggaacgctat ccgtgaattc tttctctgca ccaaaattta atacgttcat
     2341 tgaagaatca ccataataac gactgatatt ttcgccaaat cctcctgaca aactaccatc
     2401 ttctaatgtg atgactaatt cgtgtgtttc ttttaattta tctaatgttt ctacgtcatt
     2461 ttctgtgatc gttcctggat tgatcaacgt tgcttcgatt cctttttttc taaaagcttt
     2521 tactgtttct tttcctaatt caagaaaacc gcctaaagct aaaattgcta cttttcctcc
     2581 attatttact gtttgatagg ctggaatact ataatcattt gcgatagctg taccatgttc
     2641 tacaccatgt gtaggaattc ggattgctac aggctgcgct gtttgttcga acgcccaatc
     2701 tagcatagag atcatatcct cttttgttgc tggagcaaga tatttgatgt tcgggatact
     2761 tgataagtaa ggaatgtcaa atgttccttg gtgtgttcgg tcactagctg agatcgttcc
     2821 gccaccgact aaaataatca ctggattctc gttgatcgct aaatcatgtg ataattgatc
     2881 atatgcccgt tgcaagaaag tacttgattg gaagacgact ggacgtactc cctgagaagc
     2941 caaagcagaa gcgaaagtga tagagtgctg ttcagcgatc cccacgtcaa aataatgatc
     3001 tggatgtttt tcttggaatg ttttcagtcc aaaggcgcct ggtatcgctg cattgactgc
     3061 ggcaatagga gctgtacttg tttgcatttt cttatccaaa taatccatga tgatcatatt
     3121 aaatgtttct ccagaagtta cttgctttgc ctccccggtg gacagatcaa atggtacatg
     3181 ccaatgatat ttcattttat tatcgattgc cggctgataa ccgcgacctt tttccgtatg
     3241 gacgtgaagt acaatcggat gatcaatatc ttttacttct tcaaataaac ggatcaccgt
     3301 ttccagatcg tttccattat ccacgtaacg ataatcaaat cccattgctt taaataggtt
     3361 attgctgctt ttaccgttgg tttgacgaag ctcagctaag tttttatata aactgccatg
     3421 attctcatca atagacatct cattatcgtt tactaatacg attagattgg attgtaaaac
     3481 accaccattg ttcaatcctt caaaagctaa gccaccactt agggagccgt caccgataaa
     3541 tgctaccaca tttcctgatt ctttcttcag atctctcgct ttagctaaac ctgttgctaa
     3601 gctgatagat gtagatgtgt gccccactgt aaaatagtca tgtttacttt catgttggtt
     3661 ggaatatccg ctcacttcac ggtaatgtcc tttttcaaaa ccattttttc gaccagttaa
     3721 aagcttatga ccataagact gatgggaaac atcccaaacg attttatcct ttggtgaatc
     3781 aaatacataa tgaaacgcaa tcgttaattc tactacacct agattcggac caacgtgccc
     3841 acctacatcg ctgtcctttt gcaagatttt ttggcgcatc tcttctgcca actgtttcat
     3901 ttcctcaaca gataattttt tgacatctgc ggggctattg attgcatcca atacatgtaa
     3961 tactgtactc ataatacatt ctctcctccg tactatcttc ttttaatgaa aatagatttg
     4021 ttacacttta tctattctac tacttagagt tgactctaag tcaatagcat tttaaaaaaa
     4081 tttcagagaa agcttgttat ataagtagat cttcgcacga atcaggactc aaataaaaac
     4141 cttctacaga caattaaaaa gctttgtaga aggtctccta attaaggtct catcaagaaa
     4201 tatgtttttt taataggtga agttcctgag caatatgata caaatggatg tttgcacctt
     4261 ttaatgagat tggatagatc tttgtctttt cagtttctaa tccattttga tagctagcag
     4321 catcgcacaa aaccactctt tttttcgcac atagacattc atcaggcaat tcttctaatg
     4381 aacgaaataa aataggattt atttcagcga tgccttcttc cgcatacaaa tgaaattggg
     4441 ttcgataaaa aaattgtttg actgaacttt ctgaaacaac aaaaatttct atttcctgtc
     4501 tccccgcttg ttcactgttg atcaaagaaa gacttttgac gaattttcga atgaaatttt
     4561 cattccatct tagttggatt tgatttttct ttttccactc ttccaatatt tctacaattt
     4621 ttccgtacag aagttgttct ttttctgata gaagatagat cctttccggt aaaaatactt
     4681 gtccatctgc ccacaacgta cggacaaatt gtaaaaagga ttgcttgaaa ataaaatttt
     4741 ctgcatcgtc gatttcagcg atactaataa tctgtaaaat caattcattc agtgataggt
     4801 cattgagaac aaaattttta taaacaactt cgaaatcttt tcttaacagc tccatgttct
     4861 cattggtata atttctactg ttgaacaaag aaaaaatata atagacatct tcttcttgta
     4921 agtctagaat tcctttttgc tcttccaatc cttgacagac taattgaaat aacggcttcg
     4981 tttctgcgct tttcttatct tcttctttca gtccaagggg aaatgccttt cttccgattg
     5041 cgatctctat tcctcgtatg ataaaggact tttcatcttc cgaatatctt cttttaaaaa
     5101 aatgagtctc tacataatca atcaactcgt tacaagcttc ttctacatac ggatttttat
     5161 tttcatagcc ttcccaattg acataacgta ctaaagctag caagatgttc cgcgagtcaa
     5221 attctgggat aatgatttct ttatttttca aataatcatt ttctttaaaa aaatccaata
     5281 cgattttttt gattgtatat actttgctca aagaaataaa ttcaagatca gccagttctg
     5341 tactcgtgaa tataccatta aaataataac ttaaaaactt taaaaaataa gattgaccgt
     5401 acagaaactg tgtgagctca gaaagagcaa taccatgttt gaatacgagt ttatacttat
     5461 gcttctctct tacttgttct tttatacaat attctagaca ctcggctaga ctttctagat
     5521 cgtgaaggat cgttggtgta gtcaccctta gagaaatagc tgtttgttcg atgtctattt
     5581 cttggaattc aaataattgt tcacacagat acacttttct aaatatattt cgctcgatgt
     5641 aattttccag cattcctgat acacgcctcc agtttttctt atgattttat ttaatcatat
     5701 ctgtaaatcg tatttttgtt tcctttttat ttagaaaagc cctttcaaaa accaataaaa
     5761 aaagaaactg ggacaaaagt caaacaagct cacaatttca gcctcattgt aggtagcttt
     5821 gagccgtttg gacttttacc ccagcctctt tcaagaatgg ttattcaaag atgtcagcgt
     5881 ctagttcttt gactacatct ttcaacaatt ggataccagc tgcgctgtta ccaaatggat
     5941 ctagtgccgg gctgaagaca cctatgccgt aacgatttgg cacagctgcc atcaacccgc
     6001 cgcctacacc gctttttgca ggtacaccaa catggacgga aaactctcct gattcatcat
     6061 acaaaccagc agttgtcata attgatttga cgattgtagc actttcttca gtaatgattt
     6121 gtttgccatt ccaaggagca attcctttat ttgccagcac agcagctaat tttgctatgc
     6181 ctgtagcggt gacattgagg gagcattgtc tgaaataagc atcgagtatc tccggtacgt
     6241 ctccttcgat catttgatta cctttcatat agtaagcaag agagcgattg atatcacctg
     6301 ttcttgattc agaatgatag atttcttcat tcaatgtgat attaggatca tcacagatct
     6361 ctttcataaa tgacaagatt cgctcaaatt tttcatctgc atctttaccg gcaatcaacg
     6421 aggttgttgc aatcgctcct gcattaacaa aaggattcat tggatgtttg cgatgattga
     6481 tttccatatt catgatagaa ttgaaggcaa aaccggttgg ttccgtgttg attgtttgaa
     6541 agactttttc tattccgttg tctttgattg ctaataacaa tacgggaact tttgacatgc
     6601 tttcgatggc aaagcgcaca tcggcgtctc ctccctcaat cagttgtttt gtatccaaat
     6661 cataaatcgc catacctaat tgtttgggat ccacttcagc taatgctgga atgtaatccg
     6721 ccacttgtcc tgtttgataa tagtggaaat tttttccaat caattgttct aattctttta
     6781 cttccatttg aataaccagc tttcattttt tatttttctg catgctggaa tttatgacgg
     6841 catgcgtaga tcgtaaatgg aataactaag acgataagat aagaaacaat cagaatcgtt
     6901 tcataaatcc catgctgtcc agctgcgata ctagcaggtg gaacaaatga gatacaaaaa
     6961 gcaaaaagtg aagtcaaaag acctaaacaa gcaacgatta cttttacaac ttttccgcct
     7021 ggaacctgat aggctctttc aagactgtca tctttaaaaa ccagcttcaa atagcccgcg
     7081 aaaaacataa agtacaacat caggtagatt acaacagtca atgacatcgc tgcaagaaat
     7141 gaaagattat tccccccgcc gcttaatgta aagacggctg cccagattgt tgcaacgatc
     7201 ccatttgcga tcatcaaatg agcaggaaca ccgtgtgtgt tttcttttgc aaaatattca
     7261 ggaagtaatc cttcttctgc tgctacgtga agtgcttttg aaggagaggt tacccaagaa
     7321 gcaatttctc cgatgacacc taaacaaaca agaatcgcaa gaattttcgc aatccatgca
     7381 agatttggat caatgtgtct taataggaat tctaacgatt gaatcacccc tgtattaaga
     7441 gacaaaccat gttgaggtac tgtagtagcg acggtcaatc cgccaaaagt atctaaaatg
     7501 attgccagaa taaccaatac aaacattgct aatggataat cctttttagg gttttccatt
     7561 tcatttgcat gactggcaga tgtttctacg cccatatatg ctaaaataaa ggaaacaaaa
     7621 acaaccagcg taggcaattt cgaaaaatca ggaatcaatg ttttcataga cagtatcgtt
     7681 tgtagtggag cgcctgaagc gacataatgg atccctaagc caaacaagac taatgctggc
     7741 aacaagatac cagctacaaa accgatccga gcaatcaatg aagtgtattt ggttcctttt
     7801 aactgtgaga gtaaaatagc ccagaaaata atcaatagga ttgccaattt cagatattta
     7861 ttgttttgta tagcaccaat acccgttgca taggataatg caccagttaa gaaatacagc
     7921 atggtcaagt agccaactgt tatctcaaac cattggaaaa agattgcggc aaaaccgaat
     7981 cgttttccca atgtgcgact gacccaagta tagacaccgc ctttttccca tcctttcacc
     8041 gttgccatct ctgctgcgca caatgccaca gggataaacc atagcaaacc gcctaataac
     8101 aagaaaaata ctaagctgaa gcctgaggtc gcgaatgtgg gatattcata taatgaaacg
     8161 accattgaga tggtcatagc caaaaagcca aagaacgtta atttttttgc aggaattttc
     8221 gttttgcttt gttgcatgaa tcttcctctt ttctatttga atttgtttca attgagtttg
     8281 ttgccaaaga agaaaaagtc caatccttct tcttgttctt cttttgtagt agtgatcgtt
     8341 gtaaagtgca tcgccgattt taaaatcact gttgtcagca cataggcata cacggcagta
     8401 atgatgacac caatcacttg aatccctaag aagtaccagc tgcttggatt cgttagtaaa
     8461 tggctgttag atgcaaagat ccctatcaga atcgtacctg tgaatccgcc catgccatga
     8521 acaccccaca catcaagtgc atcatcccat tgtttctttt tacgaaaatc aacagcagca
     8581 tgacagacga tacccgcaat gatcccaatc agcatggcac tttttgcttc tacataaccg
     8641 gcacaaggcg taatggctgc caatccagca acggatcctg ttagtacgtc gacaaaatca
     8701 aagccatttc cttttacttt tgcaatgatc agccaagtaa tcatcgcaaa tgcaagagcg
     8761 atgaatgttg aaccaaatgc attgactgct tgttcatctg ctcgaagtgc accaccggaa
     8821 ttaaaaccga accagccaaa ccaaagcagt cctgtaccga tagcagctgc cataagactg
     8881 ctatgctgac ttctttctcc taaaggaatc tttctttgac caagtgtgag gatacatgct
     8941 aaagagccga atccagctgt cgtgtggatg actgctccac cagcgaaatc tctaaatccc
     9001 atctgatcca aaaatccgcc accccatatc cagtggcaaa cagggaaata aatcagcaga
     9061 ttccaaaaaa taacaagtat aatatagcct tttaaattca atctccctgc aaaagctcct
     9121 gtcatcaaag gaacggtaat cacgataaac atcagttggt aaagaaagaa cattaaaaat
     9181 ggaatcgttg caccatgaaa catgttgggg aagcttgtaa catgtctcat caaaaagaaa
     9241 tctgccgggt ttccaataac accgccgata tcccgtccaa aggccaatcc gaatccacca
     9301 aatacccaca taatcgttgt tatcccaacg gagagaaaag attgagacat gatcgttagt
     9361 atattattct tccgtgctaa tccgccgtaa aaaaaagcta gaccaggagt catcaggcaa
     9421 accattgctg tacataaaat catgaatgct gtatctcctg tattgatagg taatgtattc
     9481 atttttttcg cctcctttct acgattacga gtataaaaga aggtgtagtt gtttttttct
     9541 tctgtttttt tgaattaata cttgcattta taaaagacag acaattaata aaagcttgat
     9601 gtgttgtgct ctttttcgtt tttcacacaa ataaagacaa aaaataaacg gtcttctttt
     9661 tatagaaaac cgtttatttt cgtctttttt tatttttaaa gatatttttt cataaggaat
     9721 aaagaatact tcatataaaa tatataaaaa agaaccgcag cgagtgtcgc tacggttctc
     9781 aaatttgttc ttcatttcaa cataagtttt tcttcaattt gcttataatt tacgtgcaaa
     9841 gaatgaaacg actaatacta aaatgattgc accgatcaat gatggaatga tagccattcc
     9901 tgcaattgtt ggtccccaag ttcctagtaa agcttgtcca attgctgaac ctactaaacc
     9961 agcaatgatg tttgcgatga tccccattga tgatccttta ttcgtgattg ctcctgcaat
    10021 tgctccaatg ataccgccga cgattaatgc ccaaataaat cccattttaa attcctccta
    10081 actgacttct tacttcttat tgtacacgtg gttcgttatt ttctttgact ttttgtgcac
    10141 ctttgcctac agcagatttt gctttatctg tttggcgtga agcaaattgt cctgtggatt
    10201 ttgctgcttc tgttactttg tcttgtactg tttcttgatc tttttggtat tcttcttgtg
    10261 ttttgatgtc tgcaacattc acattcactt cgataacatc taaatgtgtc atattgttta
    10321 cttcagtact gatcaattct ttgatttggt tgtaaatatc ttcgatgtct ttaccgtatt
    10381 ctacgatcac atctagatcc acagctactt gtttcttacc tacttctgta ttgatacctg
    10441 ctgttacgtt atctgtgttg actaattttt cagccatatt tgagaaaaat ccgccatcta
    10501 atgtcaatag accatctaca tctttcaatg cataaccgat gattttttgg atgactttgt
    10561 cttcataagt caattcacca ttcatcatgc ggttttctcc tttttcattt gccaaaactg
    10621 atccatttgt tgtattcatg tttttgtttt ccatctttgt ttcctcctat gatttaaaaa
    10681 attgatcaac tattcctgac tgtttcaaat aaaaaccgat tcctgcgcca attgctgtca
    10741 tgattatgat caacaatgtc ttgaaaaatc caatcgttaa ccataaaaca gctagcagta
    10801 gtccaagacc cccaaagata atagggagct tgtatgctga aaatagttct ttcattattt
    10861 ggccctccta ttccactcga gagtgcttgt agtctcttgt atcttcttgt tcttcataac
    10921 cgctgtatgt gactgccact ttgaccgttt cttttgttcc gagtacttta gttacttctt
    10981 cttcgatatc ttgcatcaat acacctgttt tccctactaa tgaagaagtt cgtttcaagt
    11041 ctccgttgac atggatatgg attcgatttt tcgttgaacg tatccgtact ttaggtgaat
    11101 gaatgaactc ttcttcgtgt aaatgggaac gtaccaaccc ttcgatcgct tttttgtcga
    11161 ttgttagttt gccatcttcc cgtttcataa cgaaagttcc tcttgacttt ggatagaaaa
    11221 gaacgactag caagatgata atagcaagaa cggcaaatac tacagctgcc cagaaaatat
    11281 attgttgcat gtagtaattc gttaaagtaa agaagcgaaa tcgttccaat tgttcaggca
    11341 taatatacgg cgcattgatc aacgcaatcg ctagaaatac tgaaagtagc agtaaactca
    11401 tgataacacc gattattttt aaccccttat tcattttcca cctcctataa acttattttt
    11461 accctgtaaa taactctacc gacaatcaca gtatagatag cgaacagtta gcatagcatc
    11521 agtaaataaa gcgctaccaa acattgctgg atcataacgg cgttttattg ccagtctgat
    11581 actgaaataa caattgctta cttattctct atttactttt gattgctggc tatacttaaa
    11641 ggatattatt caagattaaa aattgcaaag attatgctca gttattatta caagattcta
    11701 tttat
//
LOCUS       DAZKOR010000090        10083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000090 DAZKOR010000000
VERSION     DAZKOR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.092"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..2229)
                     /locus_tag="ACG8ZM_002292"
     CDS             complement(109..2229)
                     /locus_tag="ACG8ZM_002292"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0493223.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="ACG8ZM_002293"
     CDS             2459..3637
                     /locus_tag="ACG8ZM_002293"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0493224.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="ACG8ZM_002294"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="ACG8ZM_002294"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0493225.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="ACG8ZM_002295"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="ACG8ZM_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0493226.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="ACG8ZM_002296"
     CDS             complement(4990..6243)
                     /locus_tag="ACG8ZM_002296"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0493227.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="ACG8ZM_002297"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="ACG8ZM_002297"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0493228.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="ACG8ZM_002298"
     CDS             7821..9167
                     /locus_tag="ACG8ZM_002298"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0493229.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="ACG8ZM_002299"
     CDS             complement(9475..9861)
                     /locus_tag="ACG8ZM_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0493230.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9910..>10083)
                     /locus_tag="ACG8ZM_002300"
     CDS             complement(9910..>10083)
                     /locus_tag="ACG8ZM_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0493231.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         3214 a         1839 c         2054 g         2976 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttattata cccgtggtgc
     9901 tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg
     9961 acttaataga acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac
    10021 agaacgtgag ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac
    10081 tgt
//
LOCUS       DAZKOR010000091         9921 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000091 DAZKOR010000000
VERSION     DAZKOR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.093"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            322..801
                     /locus_tag="ACG8ZM_002301"
     CDS             322..801
                     /locus_tag="ACG8ZM_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0493232.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            834..3062
                     /locus_tag="ACG8ZM_002302"
     CDS             834..3062
                     /locus_tag="ACG8ZM_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0493233.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3191..4834
                     /locus_tag="ACG8ZM_002303"
     CDS             3191..4834
                     /locus_tag="ACG8ZM_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0493234.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4907..5452)
                     /locus_tag="ACG8ZM_002304"
     CDS             complement(4907..5452)
                     /locus_tag="ACG8ZM_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0493235.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5475..5960)
                     /locus_tag="ACG8ZM_002305"
     CDS             complement(5475..5960)
                     /locus_tag="ACG8ZM_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0493236.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5950..6762)
                     /locus_tag="ACG8ZM_002306"
     CDS             complement(5950..6762)
                     /locus_tag="ACG8ZM_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0493237.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6901..8433)
                     /locus_tag="ACG8ZM_002307"
     CDS             complement(6901..8433)
                     /locus_tag="ACG8ZM_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0493238.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8542..9573)
                     /locus_tag="ACG8ZM_002308"
     CDS             complement(8542..9573)
                     /locus_tag="ACG8ZM_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0493239.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3304 a         1842 c         1909 g         2866 t
ORIGIN      
        1 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
       61 ttaagaatac aaaaaagatg ttggtgaagt tcaccaacat caaaaagtat agagccaaaa
      121 tattccaaca tcatcttgga ctttctacat gttactaatg agcgtgtaaa agaacgagat
      181 ggtgttttta tttctatcaa tgaagctctt tttctttgca atcctcactt caatcctcta
      241 taatgtcgca tatagtttag taaatgagta aactactaat ttacttattt actaaaaata
      301 aaaggatgag gagttaaaat aatggaaata ccaacaaatt taaaacataa accagtaatc
      361 gttgtagaag actatgataa agttgatgga agaaatgcat taaatatgga tgcaaaaggg
      421 ctttcactgg gccttgctca gtggaatgat cgtggaaaag tcgatatctc aggcaaggta
      481 tggcgacata ctggagaaaa atggtcacga cagtcggaag aattaccaat cacacgtatt
      541 ttagatttag cgatcttagc tgcgcaagga agtttatact tccaagatgc ttatcgttat
      601 gaaaaatttt atgatccaga aaatccagtc gtagacatca ttggattaca agggaaccga
      661 atgacggtag aagtagatac caaaaatcca aagatcgatg aagacatcat tttgtattac
      721 gatacactac aaaaagacgg agaactgatt ggggaacgat tccgtatttt aaaacgtctt
      781 ttagatgagc taggatatta atagacagag atgatcaaaa ggggaagaga agcatggaga
      841 atttctcact aacagaaaac cgaacctttt cacggctaga aaagcaaatg atctggaaga
      901 aaccgaaaag ccatcggaca agtgcagaag aatggcggat cgcctccgaa atcaaaggga
      961 attggcaaga tcctgaaatg aaaatcagta acgtactttt ggaaggagat gccgggtcgg
     1021 ggaagacaca attagccaaa gcactatctg ctgatctgca acttccctat acaaaagtga
     1081 cctgttttgc agatatggac aaatcagatg ttttcggagc cttattacct gtagtagatt
     1141 ctaataagga acaagaccaa gaattactag aagctattta ccaaacagat acacttgaag
     1201 ctgtactgac actgatccag aaccattacg gcattgatcc tttttctgct aaagaaaaat
     1261 tagcagagct agttcaaagg atcgaagctg attatccaga tgcaatccat tacagctatt
     1321 acccttctga aatcgttcga gcgattgaaa aaggctattt attagagatt caagagccga
     1381 ctgtcatccg agatgcctct gttcttgtgg cacttaactc agcgctcgaa ccaaatggga
     1441 tgttgaattt accgacagga atcattcgtc gccatccaga ttgcgtcatt gtgatcacta
     1501 caaatcgaaa ttaccaagga aatcgcccat taaatgaatc tttaagagat cggatgcaac
     1561 atgcggaaaa aatggatctt cctgccttag aagtcatggc agaacgtgcg atggcgaaaa
     1621 ctatgatcca aaaacaagaa cttctcttaa agatggcaga gattatccgt ttgttggatg
     1681 aaactgcaaa agctaacgcc atcaaaggtg tagcaggaat gcgttcttat ttttattggg
     1741 caaataccta taagcaagga caagatttac tccagtcgat ttatccaaaa gttttgtata
     1801 aattatccac tgattcagat gaattggcaa ttcttgaaca agctttgtct gacagtggat
     1861 tgctactgga acttcaggac atattgagaa aagaaaaatg gggtcatcct caatctgagc
     1921 atacaaaaga aagaaccatt agcgccgaag aagcaaatga acgaaacatc actgaaaatg
     1981 ctgaagaaat tctgcgctta gatgaaaaga aatcgttgtc tgctgaagaa ccaaatgagg
     2041 gagaagagaa acaagaagaa acaatagaaa aaaacgagat tcctcctcaa atgcaaaaag
     2101 aacgcagtag cgatatcgaa ggaaaaaatc aaaaagaaca acaaacagga gatgattctc
     2161 aagaaatgtc tgcttctgac atggaagcac atgataagga aatcaagaag caactgaata
     2221 aagaagcgcg acaaatcatg aaggaaacga ttcatgaaaa agaaggtctc atcgttcacc
     2281 gaccagcttt ccaaggaaaa acgccggaag cacgtgagct acacatgcaa gtaatgccaa
     2341 tagtagaatc acttagccgg caaattttag agttactgga taatgaacaa acagagacgt
     2401 accaaaaagg aaaatacgaa ggtcagcggt tcaatgcaag caaagtagca tatggtgatt
     2461 tgagaaattt tgacaaaaag aacccgccac atgagcaacc ttctttagcg gtagctgtac
     2521 ggatcgatga atctgggtca atgatacggg atgatcggat cgaagcggca aaaaaagcag
     2581 cgattgccat tgccgaattt tctaagaaag tcgatatccc attgttgatt tacggagata
     2641 cagcagaccg ttcgtcaaga gaaaagactt ctttgttttc ttataaagaa tttgaagacg
     2701 gatttcaatg gttaaatgaa aaattagtca ctatgaaacc aaggcaaaac aatcgtgatg
     2761 gtgctgcact tcgtttagca gcagaaaaat taaacagaca atccgcaaca accaaactgt
     2821 tgcttaatat tagtgacggt cagccaaaag cgttgccgga ttatacaggc aaaaaagcaa
     2881 aagaagatat ccagtctgtt ttgaaagaat atgaacgtca aggaatccta tttgtctccg
     2941 cagctattgg ccaagacaaa gaagaaatca agtcgatcta cggtgaaagt cgttttgtag
     3001 atattactga cttgaatgaa tttcccaagc aaatgattca actgattgcc cgctatctat
     3061 gataaaataa agctcaacga acgcacttag cagattcgtt gagcttttcg tttctagtta
     3121 ccggtaacaa ggatacttct aaaatagaat cggttataat caagagggac cgttaaagga
     3181 gtgaaacgat atgatacaag aacaaaaatg gtggaaagaa gcagtaggct atcaaatcta
     3241 tcctgctagt ttcaaagata gcaatggcga tggtcaagga gacatcaatg ggatccgtga
     3301 aaaacttaat tatttgaaag atcttggtat cggctttatc tggatcaatc cgatctatca
     3361 atctccattt gtcgataatg gttatgacgt aagtgactat caagatatag aagagagatt
     3421 cggaacgatg gaagattttg atttattgct aaaagaagca catgaattag ggatcaaagt
     3481 gattatggat cttgtcatca accatagttc ggatcagcat caatggtttg aagaatcgaa
     3541 aaaatctaaa gaaagcccgt atcgcgatta ttacatttgg gtcgacgggg tagatggcaa
     3601 agaaccgaat aattggacat cgattttcgg cggttctgct tgggagtatt ctcatgaaac
     3661 tggacagtat tatctgcatg tttttgcaaa agaacaacca gacttaaatt gggaaagcga
     3721 gaaattgaaa gaagaactat tcaatatgat ccgatggtgg ttggataaag ggatagatgg
     3781 atttcgttta gatgcgattt ctcatgtaaa aaaagatgaa tattcagtaa aagccacaga
     3841 gaaccctttc tcaccctttc aaaatgtctc aggaatcgaa gaacatttga ccgatttgaa
     3901 acatgttttt gaagagtacg atatcatgac tgtcggagaa gcaagtggtg tcactgcaga
     3961 agaaggacca caatgggtag ggaaagacgg ttatttcgat atgatattcg aatttgatca
     4021 tatccatatc tggcagcaag aaaaagaagg gcaattagat gtccttaaat tgaaacatgc
     4081 attaagcgct tggcaaactt cattggatgg tatcggatgg aacgctttat atatggaaaa
     4141 ccatgatgtt cctcgtgccg tttcagtgtt tggagatact cgtcccgatt tttgggctat
     4201 gtctgcaaaa gcaatcgcta tgatgtattt ctttttgcaa ggtacgccat ttatttatca
     4261 aggacaggaa atcggaatga cgaatatgcc ctttgaatcg atcgatcaag ttgatgcagt
     4321 agattcgaaa agactttata aaaggctgct tgcggaaggt aagacaagag aagaagcatt
     4381 ggatatcatt cgtgaaacaa cgagagacaa cagcaggaca cccatgcaat ggacttcgga
     4441 acaatatgct ggtttttcta cacatgaacc ttggctgatc actaatccaa ataccaaaac
     4501 gatcaatgta gaacaacaag aatatgaacc agagtcggtc ctgcaattct ataagaacat
     4561 gatccgcatt agacaaacca ataaaggtct gatatacggt tcttataaag aatatcttca
     4621 tgaacatcca caactttatg tctatgaaag atatttagaa gatgaacaat atcttattat
     4681 ggtaaactta acagaatcac ttgctgacta tgaacttcca aaagaagcag atcagtcctg
     4741 gacattactc ttgtcaaatt cctctagcgg agaatttgaa gctaaaggca tactagcacc
     4801 ttatgaagct cggttatata aaacaaacaa gtaaaacttc ctcgttaaag ttcattagtt
     4861 ggactttgcg aggaagtttt tcagattgct ctgtcaatta ctcatttcaa taattcgctc
     4921 taggaccacc gatatattta ggacgagagc gaagggctgt cacgtgagct tgtccaaggg
     4981 ttttcaaacg aggtctgaca ggtacttgga catgcttgat atgcataccg attgatgtgt
     5041 caccgatatc gataccggct tgtccaacta cgtgttccac ttcgacagga tcattgaact
     5101 ggtcaaaagc agcaacagaa caagcacctc cagcatgtag tgcaggaaca acagagacaa
     5161 tttcgaaatt ttttgcttct gccactgttc gttctactac taatgctcgg ttcaaatgtt
     5221 cacaaccttg gacagctagc gcaatttctt taggatccaa caactctttt aacgtacgga
     5281 tgatccattg accaacttcc gcactggaat tttttccaat atgtcctcca acgacttcgc
     5341 ttgtgctgca tcctaaaaca aaaagatcac cttttttcaa gtgtgcctca gccaatatgt
     5401 cattaactat ttcagtcaat tggtctttta attctttttc tgacaacttc attttctaaa
     5461 accttctttt tcaattaata ttgcttccgt gcttgaatac gttgaagact tgcagttata
     5521 ggaaaggcaa gtcccattcc aatcagattt tggaagatgt ttcctggtat agaagccact
     5581 ccagccggcc aatcatataa aatccaaccg gcgataccat agccgatgat cataactaag
     5641 cttcctaata aaagccctaa tccccacata cgagtagaag atttgctgaa agaaccagca
     5701 acatatcctt gtaacccgtg gatcactaat gaaaaaactg cccactgcgg atatcctgaa
     5761 atcaaatcga tcaatagacc actggaagca ccaacaacta atccacccgt tggaccataa
     5821 agaaaagcag cggtatagat cccggcctcg cataatgtca caaatccatt cgtagccggt
     5881 acagggatca aaattgttaa tgaaagggca actgtcaacg caatcagcat agcatagatc
     5941 gttaattgct tagttttcat ttctcattcc tcctttttga ataccgccag accagacact
     6001 tccggtatga tcgttcatca gcacgccatt agaaatacaa tgggcaacaa attttttact
     6061 ttctgctact gctttggtaa aatctgcccc taacacaaga ttcgctgtta ttgcagaaga
     6121 aaaggcacaa ccggccccgt ttactgttgt agtgtcgatt tttttatgag aaaatacctc
     6181 ataatgatca attgaataga gaagatccac agcttcatct ccagccatcc ttgtgccgcc
     6241 tttgatcaca atgttttttg ttcctgaagc agtgagttct ttagcgagat ggatcatttc
     6301 tgttaacgaa gagggaagag cttgaccaga aagtaaagca gcttctgcca aattaggcgt
     6361 aatcatcgta acaagtggaa aaagttcttc gatgagctgc gaaatataca attgattgat
     6421 cacagcttca gtttctttga atgccaaaac agggtctaaa atgattggga tattttgttt
     6481 tgtttttatg aagtctcgaa caaccatgat tgcttcactt gaattcaata acccaatttt
     6541 gataccatct aatgaagtca ttttatcaat cgaaactaac tgtcgtttaa gcaattccaa
     6601 agatatagat tccagcataa atcgctcatt aatatctagt gcccctatag aagtcaaggc
     6661 gcttagtccg aatagaccaa aattctcaaa agtttttaaa tctgtttgga ttcctccacc
     6721 agcaaatgga tcagaaccgc caattgtcaa gattcttttc ataacatttt cctccatgaa
     6781 taagagtata acgaaatttt caaaaaagaa aaacagccaa ttggcatatt tttatctcat
     6841 ccaatttaaa aaaacaaaaa gaaaagaaag accaaaatgg atctttcttt ccttaaatag
     6901 ttaggacttt tgtgatttta attctttctc gatttgaagc cgttgcactt tcattgtagc
     6961 tgttcgcggg attgcatcaa aatcacggat gatagggacg ttcaagcgag gaagttcatg
     7021 gacttgctcc caccatcgat tccaatccat ttctttatca ggagctaaag caataatcgg
     7081 ttgtggagaa tgatttttat cccttacaat cactacttct gccaaaaaat ctaacgaatc
     7141 taataaaaag tcttcaatag ctaatgtact attgatcgtt tcgatcaaat ccacttgccg
     7201 atctttcaaa aataggtggc catgcttatc cataaaaccg taatctccgc tatcccacca
     7261 atcgccataa acattttcct ggaaccgtgc atcttccttg taataagtca aagcccgtcc
     7321 tttagacagt aattgtatat gaccatctgt gttttcaggt aggacattcc caactgaatc
     7381 tgtaatccga gcgcttgtca tgtcttccag tccaacgccc atatctcttg catcacttgt
     7441 tttcaaactc tctagtgtat gagccttaag aatcattggt ccgcattcac tttggccata
     7501 tacttgaaga aataccgcat cctgtgccat cgacgttcgc aaaaatgtct tcattgtctg
     7561 gttgtttata gcatcaaaag tggagtgata gtatcggata cccgaaaaag cttctggatg
     7621 ctccttagca gtaaaacgcc actgaacaaa gttattcgga tgggtctcta gtgcaattgg
     7681 ccggtgagct tccaacatat gaacaacttt tgaactttgt gcgtttgcta aaggcatcat
     7741 aggaaatccc atggacatca aagaagagat accaatattg aatctggaat gcactggaga
     7801 gatgtgaaaa gcgattaatt tcttttccga aatttttgtg aaaactgttt tttgccattt
     7861 cgtccgccat cccatagaat gagcagaatg acaaataagt ttagggatac cagttgtacc
     7921 agaagtatgg gtcatataag aaatttcatt tttgtctaaa aatgattgag aaacagacaa
     7981 agcaggttgt tccattaaat aaagaatagg aatctgtttt gattttgttc catttgaaat
     8041 ttctctcact cgcttttttg tctcatcatc aaataaaatg aaagggtctt ctaaacgatc
     8101 gataaatact tccattgttg ttgttggtag atgataagaa gtcatgacag gcactgcacc
     8161 aaggtaagac acagaaacag ctagaaggta tgtgtcaaaa gcggaactct tataaataat
     8221 aactttgtct ccggatttca ctccaagatg agccaattga taagcccgtt tcaacacact
     8281 ttcgcagcat tttttgtaag ttgtttcaag tcctaattct gggaaagctg aaagtggctc
     8341 atcaaagata atcggggtct cggaaaagtt agctgcagct tcttgaaaat tcgtaaataa
     8401 gttcaaaggg gaatacgtta tggtctccat cattcacact ccattatctt ttgttaagta
     8461 atatacaaat tcaagataat cgtaacataa tcctttatat aataacatag tttattgtga
     8521 aaaaaagatc atacatttgt tttatatgga agaaacccga acgaatgccg ctaattcgtg
     8581 ataagaaaaa tcaaccatct tttcccagat atcttgaaaa gaatgggatg agaggtatcg
     8641 attaccacat aaataattta ccgcgtcagc atcatctaaa tatagttctg cataataacg
     8701 atatgttttt ctgcatagtt ctggcgatac gtttttatat actgtaattt tgctcaaaat
     8761 ttggcgaata gtccgaggag aaatttgctg atcatgtgag gaacgaaaaa caaaagaatt
     8821 ttcgtccgta ttttgtgctg ttaacttaaa atagttctcc agaaatggca tcgcttcttt
     8881 tggtattggt actttattca gtctgttttc ttttctattc agattaagaa tctgcttggt
     8941 caaagataca tctttacgtt taagttctac tacttcatgg atctttaatc cgcaatacag
     9001 aagaatggca gtaattgcaa ggttcctcgg tctattcttt tcttcaaagc tagcttctct
     9061 gcttgtttga aagccttcaa tcgggataaa caaatgctca aaatctttga aaagttctgc
     9121 atcgatatat cgcggagctg ctttggttgt ttccggtaaa cttgtaccca aaagagaaat
     9181 ctcttcttca atcagtcctt tttgatacaa aaatcgtaaa aaatgattta atgaatattg
     9241 cttccttgaa atcccgtaat agctattttc aaacaattgc tccgtttctt ggttcgctgt
     9301 agtttggaac gaacgagtat gtcgatagat tttagctaag aaattctgat aatcttgttt
     9361 ttctaaatgg ataatcagct gatcatccac ttcttcagaa aatacttttc tcaaataatc
     9421 agtaaacaga tgaaaatctt ttgcgtattc atagcgactg cctaatgaaa gggaagaacg
     9481 attttcttct aaaaactgtt gaaagacgga agggagcaag tgtgtacgtt gctttaactt
     9541 ttcttgcagt tgttttctag ttttgctgtc cattacaaat tccctccaat agtgttcatt
     9601 ttctatattt taccgcattt catcaaatga agatagacag tttgtcttga tcttaggaag
     9661 caaattttta actaagtact agctaaaaga tatgaagtaa gaagatacat ttttagttat
     9721 tttattagtg cagtcattct tttgatcttt taattgattt atcctctttt tagctatgtg
     9781 gttacttcat gagcaatgaa atggatttgg ttcgttctaa tggcaggagg ctctttcttt
     9841 tatttccgaa aaattagaaa acattttttc agaatcgatt ggctctccat ttattcaata
     9901 atggtttgtc taacgatatt g
//
LOCUS       DAZKOR010000092         9892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000092 DAZKOR010000000
VERSION     DAZKOR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.094"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            276..482
                     /locus_tag="ACG8ZM_002309"
     CDS             276..482
                     /locus_tag="ACG8ZM_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0493240.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(684..1685)
                     /locus_tag="ACG8ZM_002310"
     CDS             complement(684..1685)
                     /locus_tag="ACG8ZM_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0493241.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1690..3603)
                     /locus_tag="ACG8ZM_002311"
     CDS             complement(1690..3603)
                     /locus_tag="ACG8ZM_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0493242.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3771..4277)
                     /locus_tag="ACG8ZM_002312"
     CDS             complement(3771..4277)
                     /locus_tag="ACG8ZM_002312"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0493243.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4437..4877
                     /locus_tag="ACG8ZM_002313"
     CDS             4437..4877
                     /locus_tag="ACG8ZM_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0493244.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4903..6018)
                     /locus_tag="ACG8ZM_002314"
     CDS             complement(4903..6018)
                     /locus_tag="ACG8ZM_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493245.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6063..6419)
                     /locus_tag="ACG8ZM_002315"
     CDS             complement(6063..6419)
                     /locus_tag="ACG8ZM_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493246.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6717..7691)
                     /gene="sgrA"
                     /locus_tag="ACG8ZM_002316"
     CDS             complement(6717..7691)
                     /gene="sgrA"
                     /locus_tag="ACG8ZM_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0493247.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7887..8726)
                     /locus_tag="ACG8ZM_002317"
     CDS             complement(7887..8726)
                     /locus_tag="ACG8ZM_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0493248.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8911..9798
                     /locus_tag="ACG8ZM_002318"
     CDS             8911..9798
                     /locus_tag="ACG8ZM_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0493249.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
BASE COUNT         2981 a         1979 c         1490 g         3442 t
ORIGIN      
        1 aataaaaaag tttaggatac atcgaatttt tggggaaatt cttttggggg tatcctaaat
       61 cttttctatt atattcccat attttctatt attggtctag acgtatgatg gtaaactttg
      121 tacctatctg attgctgaat cattaattgc ttatcattag ccatctttgt ttcaagttcg
      181 ctagatgatc attttattca catcctaaca aacttgtcac atttatagat tcttaatcta
      241 tttatcatac aattataaaa aaggaggtgc tgcttatgaa agctatccat atttctttgc
      301 tttttttcgc tagtgcattg attttatttg ctattcatct ttgttcccag ccttatatca
      361 ataacaacgg aatattagtg gaaccagcat tcttttgctt accactcggc tttttgtccc
      421 ttcttgcctc cgcaggcttc ggatttgttg ctttttttaa acgatcaaaa caacaaatct
      481 gacttataaa atttacgtaa aagagatcgt gacaataatc gtgtgatatt cccaaatttc
      541 gaataaaagg tgccccttgc catctctaac tcctcagtca caagttacaa tattcataaa
      601 ccatgaaaag cttgtttatt gaaattgctc cttgtgactt ggagctaagt gacagattac
      661 aacctctttt gcatcataag actttactcc ttgaaaaaca ttactaactt ttgatatgtt
      721 tcttgcgctt gtttattgct gaaatcaaac tgatattcat ggttcaaaac agcatttttc
      781 tccgcataat ctacttttgt gactttcacc cccgctttct caaatacaga agttacttct
      841 tccatttgtt tcgtaaacgt atttgtattt ccatccgtta agaatgtgtt ttgcgtccaa
      901 cgatccgcat gtctgaccaa ggctaaactt ttgaccttct ctgattgttc aaagtcctta
      961 ttatcaaagt aagcccatcc acatttttca taaagaaagc tagagatttt atcacttact
     1021 tgagtaagtg cagaaggatc caacagacca gacaaagaaa taaacttcgt aatctgcttg
     1081 ttctccaaag tcggatcaag atgatcagct tttttaattt cttcattttg aagtgctaac
     1141 aaatattctc cactaatttg tgcacctgcc gaatttccac caatcgaaat attcgaccaa
     1201 tctgcttgat cttttatctc ttttttagct gcttgaatgg cttcgttcaa ttggataact
     1261 ggtgtaggat gttttgcttc cggtgatcgt tcataatcta tatttacgat gatttgctgc
     1321 gtgtctgaac atagatattc caaataatca cgacaatcat ttttatcccc tcctacatat
     1381 gcgcctccat gcatccagaa aagaatcggt aattttttct tttccacttt cggtgtgtag
     1441 atatccatat tattattttt gtactttgaa ggataggata tttctttttt tatatcgatt
     1501 tgttcgattt tttgctggaa atcatctggt cgggcatata cttgttcttt ttcacttgtg
     1561 tcaaattgtt ttctaacaag caagctaatc ggctttggtg tgatttgatt aatcaaaaat
     1621 acagtgacta aaataactac gatgataact ggtactgcaa tcactattcg cttgaaccat
     1681 ttcattcatt taccctctct ttcttcgcca aatccatgtg ataatcgtgc taacagataa
     1741 aaatccacct gaataaatga gaagcgaatg tttcttttct ccagttttcg gcaacattct
     1801 tttttttgca tctgtctttt tttgtacttt cactgtcatc tgaatcggtt gcgtatcatc
     1861 acttgattga tcctctcgag gttcattctt tcccttattg tctgcattct ctgtcggatc
     1921 ttgctttttt ggtggagcta ctgggatttt tttgtaatag aaaataatag ttggtatcgt
     1981 ctcattaaaa atcccttcag tcggtccttc aacatttgct aattcgtatc cttcgaatgt
     2041 cttttgctct gtcatataat tttctccata agctccgtaa agctggattt cgtcctggtc
     2101 tttgagtaga cttttattgt tttcatccac atactttacg tttatctgat catggaacac
     2161 tactttcaaa aagtccgtta catgtttcgt gtaggcagca tggtcaactt cctgtgcctt
     2221 tccatgccct gctccttcta ctaataaaag atctttatac cctcctgata actcatataa
     2281 tttttgcgcg ttagacacag gtactacatc atctgctgtt ccatggataa tcaatttagg
     2341 caatgaatct gacattccaa tcgtaggtag agcagctttt gtatccaaat gcatctcctg
     2401 atcaaaatag tattgtttca aaaggcccat aaataaatct tcatgctctt gatccccaat
     2461 gatgtctaac tgatttttta tggcatccgg tagtgcgggg gctactgtct ggctgtacgt
     2521 aaaacgtagt tgttcatcaa ttgatgcaaa tccgcaattt tcaataattc ctttgacatt
     2581 gacaggaata tctttcgcta atacagacat catcgtagct gctcccatcg aaccaccata
     2641 aagtaaaatc ttttgagatg gccgctctcg aacttcttga tttatccagt aaaggacgtc
     2701 atctgactca tattgtccat aggtcacata atcgcctccg ctattaccag tcgctctgga
     2761 atcaacaaat aataaattat acccggcctt ataaaaatca ttgtattctt ctgtaacgat
     2821 cccgttattc caattccctc gaaatccgcc atggatcatg actgttttat ctgaaccttg
     2881 atcgacataa tatgcatata catccatcat tttgccatct tgtttgttta gtacagtttg
     2941 atgaagttgt tttgtatttg gatagaagcc tgtgttttct gtaggcgttg tcccatcatc
     3001 tgtacctcca agcatcgaca tcaacacact gttttttgca aaagcgatat cgtacaatag
     3061 ataagtagca atcagatgtt tgataatctc tttatctaca ccataatcat taacagcaag
     3121 ctcagtgact aataactcta ttaattttga cttgatgtct tctgttggtc cattaagtat
     3181 ttctgtcatc tgtttaccga agtaattcaa atattcttta ccattgaaat tttcaccttt
     3241 accgatatca tcattttctg tcccattctc tttgtcactt actttagcag aagaggattc
     3301 gtcgtttgac atttttgtag ctgttgcttc ttctgtttgt tggttatctg tggtagcggt
     3361 cgtttcttga tctgtgctag taacagatgt ctcaaccgtt gtttcagtat ttgtttctgt
     3421 ttgtgaagta ctcgatttcg tttcatttcc agcatcagta gtttctgtgg tttctacctc
     3481 ccgagattcc gattcggaag aaatgctctc tccatatact gtccattgcg ggacgcttgt
     3541 acttacagca aataagatga ccaacgctcc ctttaatact tgttttgttc tcaagctatt
     3601 cactcctttt tatgtttcta ggagaattat atgtaaacgc aaacattttt tatacaaaaa
     3661 ataaatggtt tatcccataa aaatagaaca ggattatttt ctcattttac gtaataaagc
     3721 agaatggata aaccagtatc cattctgctt tttttaaacg aagaaacaac ttattgctcc
     3781 aaaaatgtca gaaatcgttt atcccaaatt ttctcgtaaa aagcgattgt ttcctttgct
     3841 ttcataaaag gattatcata agttgtcgtt gctcctctac acatcataag ctggtatcct
     3901 aatccatgag caaacttcac agtcgtatct atacaatact ctgcttgtgc cccacataat
     3961 tcgatcgaat gacagtccaa ttcagttaat aaaacactca aattagtctt ataaaatgaa
     4021 ttcgcatggg tttttctcac aaaataactg tgctcaggaa tagataattc cgacaatagc
     4081 tcccatggtt ttgtttgtgg cactaattga gcatcttcgt gttgaacgaa aataactggt
     4141 ttcccaatcg tttcgtatgt tttgattctt ttattcacta atgctgtcac tgtttctaat
     4201 tcgtgtatcg gcgaagaatc aaaacatacc cctttttgta aatcgatcac tactattgcg
     4261 tcggctgtgt ttgacatact tgtgccttct ttcttttttc ttgatttaat agtatttctt
     4321 atctagctag ttccattata cgtgacaaca ttttcttatt ttacacctct aaatggcact
     4381 tttcgactat ttcttcatgt tattatttca taaatactta gctattggag gaatgtatga
     4441 tgattaaatt gtatacaggt tctggctgct cgtcttcaag acgcgcaaaa cgttggctta
     4501 tcgtccatga tataccgttt atcgaacaga acttaattcg tcaaccacta accaaaaatg
     4561 aatttatcca cttactaaaa ttgacaaaca acggcgtgac cgaaatgctc tctactcatt
     4621 ctattagtta taaaaatctt gttcacagaa tagatgaact cagtttgagt gaactatata
     4681 ttgagattcg tacatcccct tctttattga aaaaaccgat tatctacgac caatctgcaa
     4741 aacttttgac tggatttagc gaagataaaa tcaggatgtt cctttctaaa actaaaagaa
     4801 agcaagagct caacgaactc ttgctttctg gtcatcttgt cgattttaaa gaaaataatc
     4861 tttttgcaat gtcttaattt tggcataaac aatctgcttt tcttaattaa cgaaatccaa
     4921 tttttccaat atggatctat ttttctcatt cctgattttt atgatttcag ctttcagacg
     4981 cgcccaatca ttttttgtag gttgtccatt ccagtaaaat atctgtgtat tcgtttgatc
     5041 atatgccttt ttgtcgatag gataatcgct aatgacaata tcaggtaact gatcagcgga
     5101 tgcactatat tttattgggc gttcagtcat agataatatc cttttctttg cccataattc
     5161 ctgtgtttta ccatactttg attgaatata gaatatgaca ggttgttcgt gttttataaa
     5221 aatatccctg actaacatac agtattgaaa aatcaaccgt ctgttctttt cataataagt
     5281 attaagctct ggaattctgc gagctacccc actaaaaaac tgttgcatga ttggatacat
     5341 cactggaaat atatgctgaa aatctctgtc ttctgctgat tttccaaaag catccacctt
     5401 cttttttgtc ccaaaaaccc ttgaatacaa atggatcatt gccaaattga catatagata
     5461 atttttttct gaagttgtaa aacaaatctt gaagtttttt tcaaaatttt ctacaataga
     5521 cgattcaatc cagccccaac tttcttgtac tttatctgga gtatccaaca aatcttcttc
     5581 caaataggtg ttcattacac caaacataaa ataaaggtaa gcttgctctg ttggagtgat
     5641 gtgattcatc gaccagaagt ttgcttcaaa aaaaggctct agtatgtaat gcgtaaactc
     5701 ttcttttgtt atcaatggat gcacaaattt gttcatctct tctgggacat acgtaatcga
     5761 gtggccttgt ttcattcgtt gcaacgaaat tcttagtagg ctgatccatt tctttctatc
     5821 tatactacgc gtatatggcg catgtttcgc aaatttttct aatagttgac gtttattttt
     5881 tatacttgat aatatatcag aatgattttc gtcgaatgac tgccaataca tcaagtgaaa
     5941 aaagtatcgg atacggtatt cttctccttc tatcggatta ccttttttca agctgcaaga
     6001 taaatgatag tttttcaaaa tagtattaag ttctcttaat tttctttgaa tcgtacgcaa
     6061 acttatataa ttttttgact aagattttct aaggattgat aagtaccttt gtaaagtgaa
     6121 tccagtaaaa tataaccaat cgttccctct aaaagtatag aataaacttt actcagtaaa
     6181 aactgagaag aaaactcgac ttttatcatc ttgttttctg aaagaaaaga taatttatat
     6241 tctggatacc tttcgcaaaa cgattctgct tgttgaatca ccgaattcat cttagccagg
     6301 gtgtagttta gatctaaagc gatatccttc accacatata atttctcgtc tctttcataa
     6361 caatagttca aaaattgata tacttgcctt tcattgatat caataaattc ttcaaacaaa
     6421 gtacatactc ctttttatct attatttaga cttattccga cagctacatg aataacgtac
     6481 aaatagattc gttactgatt tttcagagat actaccatgc gcagtgtgca ttgtcaatta
     6541 ttcttgctct tattttccat ttgtgttcct gttatttcct gtttctcaaa tatgttagta
     6601 aacaaaaata gatctggcaa agctacaact tcgccaggca atacagcact ctcgaatgaa
     6661 atattcactc aaaaatctat cgttttattc aattttagat ctgttgcttt tattctttat
     6721 ttcactcgat ggaagagaga acgaaaatag cttgctccta caatcaaaat gagtaacaca
     6781 cccatccatg taattagctt gttcaaggtt ctactaccag tttctggtaa atcgcttttt
     6841 ctattatctg tttctttcgc tttttcttga tgatcaattc cacgagaaac aaaatcaggc
     6901 tgttttagca cactttttag gttgctccct gcatttctct tttgttcctt agttggtatg
     6961 ataggtacca aatcaagttc attatttact tttcctgtgc tgctttcgct gctcgtgctt
     7021 cctgtgctac tctcactact catgcttcct gtgctacttt cactactcgt actctccgta
     7081 ctgctttcgc tgctcgtgct tcctgtgcta ctttcactac tcgtactctc cgtactgctt
     7141 tcgctgctcg tgttttctgt gctaatttca ctgctcgtac tctctgtact gctttcaccg
     7201 ctcgtgtttt ctgtactgct ttcactactt gtgctatccg tactgcttgg cggtccaaca
     7261 actgtccaac tgcttggttt caaattctcc gtgatcgtca tctgtccatc ataaaaaaca
     7321 atcaaatcaa ctttatcttt actcttgatc tcatcaatcg atagttctgc agaaatcatt
     7381 attgtatact caataccagc ggtccattgt acgtctttta aataaacttc cccaataact
     7441 ttttctgaac ttccattacc atcccaacca tatgttagaa cagcatgtac atcactagca
     7501 ttcacgcttt tgtttggttt gactggaata gtaatgactt gttgtgtcat gtcataaaca
     7561 atccgatcag ttttcacagt aaatgaaaga ttctcatttg cccgttcatt tgcagaagta
     7621 ctgattccaa tcattgctgc aattatactc agcccaagaa taacaaataa tacggtcgct
     7681 gttttcttca tttctatcac tctccatata attaccttct atttgataaa ggaattatat
     7741 aaaatgaaaa tgatgagaac acgacagtca gttgtgaaaa tctaacaaaa gatgtcgtgt
     7801 attcgcttga atttgtttac tttttcgttt aatcaatgac agacttaaaa aagcaagacc
     7861 gatatcagcc ttgctttctt catcttttat tgataaacag cagtgtattt cttcatccgc
     7921 tttgttttat atgcatagag taacaaacca acaatcaaga ctaacaagaa taatgtagtt
     7981 gatatagatg cttcagcccc aaagtttccc ccagtaatta ttgtattttg cgattggatc
     8041 agagtggaga aaaaaccttg ttcttgcgtt ccgctgactg gtatacccaa aatagctcct
     8101 tgtgtccaat tccaagtaat atgagcacca atagccgtaa ataaacttcc tgtactgtag
     8161 ataagtgcgc tgaaaaagca acctgctaaa aagatattga aaactgccag aaatgtcatg
     8221 ttaggattca atccgtgcaa aagagcaaat aatgcagcag atccaatcat tgctattaac
     8281 ggaccaaatc taagcatctg atgctgaaaa aagccacgga ataaaagctc ttctgatgcc
     8341 cccactgcaa taaataaaat aaaactcgtt atcagcggca caatgtttgc gtttcctgaa
     8401 tctaaccgat acgcccccgt caaatataat ggactagtaa taagcagaaa cactccaatt
     8461 ccaacaacga gacctcccgc taaatacccc cagtcttttc ctcgaacaga aacaccataa
     8521 tcttctattc tttttccttc gaccttacta gcaagaaata aagctagtaa aaaaacagct
     8581 aaatgtccga ccgtaccggc aattgctcca atccattcac tatttccaat aaacatatcc
     8641 gcaagtgcat tattgacaag aattcctaaa acaaaaccaa caaagactat taaaacagat
     8701 ccaatcaacc gaataatcct gatcatcttt catccctcct ttatattaag tttaacaacc
     8761 cttttctttc ttgtctcgtg aaaactatgt aaatgttctc catcatagag agaatagatt
     8821 tttacgaact aagaatatct acagcaacct tacgaaaatg aaagactgtc tccctaattc
     8881 ttctgttaga ctgtttttaa taaggaaagg atgggaaatt ttttgaaaaa acaactacta
     8941 attatgtgcc tttcagcatg tgcaatttta ctattctcag gttgtagcaa gtccaatgaa
     9001 acgattgcaa cgtttaaagg tggaacactt acagttcagg atttttatga taacccaaaa
     9061 gttaaatcaa ataatcaaac actattaaaa aaaatgattg tcttcaaagc atttgatgat
     9121 gtatacggaa aagagatttc ttcaaaggaa gtaacaaaag aatataatga acaaatcaaa
     9181 aaactcggtc caaactacaa ggaacaatta aaagcagttg gccaaacaga agagacatat
     9241 aagaaactct ttaaacagat gctggctttc caatacggtt tgaaagccaa tgtaaaatta
     9301 acagataaag atctagacac tgcttggaaa gaattctatc cagaagttag tacacagatc
     9361 attctatttt cgactaaaga agaagcagat aaagcaaaaa aagaagcaaa tgaaggagaa
     9421 aatttctcca agcttgttca agcttatgga aaaaataaaa cattaaagga aactgatgga
     9481 aaaatgaatt ttgattcaac caatccagag attcctactg aagtaaaaaa agcagcattt
     9541 aaactaaaaa acggggaagt ttctgatatc attcctgtaa cagatccaac gacttatcag
     9601 caaagctatt atctagtaaa aatggtaaaa aaacaggata aaggttctaa taaagacaaa
     9661 tacaaatctg agctagaaaa aattgctacc gaagcaaaaa ctcttgatac ggaattcatg
     9721 gataaaacaa tccgtaaagt gatgaaaaaa gacaatgtaa ctattaaaga tccttatgta
     9781 aaaaatatat tcaaataaac aaatttgacc caattgggga tgtggcaaaa tttgagaagt
     9841 gatttttgga aattccttct tatttttgag gcaaaccact tttgccacaa ct
//
LOCUS       DAZKOR010000093         9869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000093 DAZKOR010000000
VERSION     DAZKOR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.095"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..263)
                     /locus_tag="ACG8ZM_002319"
     CDS             complement(18..263)
                     /locus_tag="ACG8ZM_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0493250.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(377..955)
                     /locus_tag="ACG8ZM_002320"
     CDS             complement(377..955)
                     /locus_tag="ACG8ZM_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0493251.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1032..1562)
                     /locus_tag="ACG8ZM_002321"
     CDS             complement(1032..1562)
                     /locus_tag="ACG8ZM_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0493252.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1566..2426)
                     /gene="rlmB"
                     /locus_tag="ACG8ZM_002322"
     CDS             complement(1566..2426)
                     /gene="rlmB"
                     /locus_tag="ACG8ZM_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0493253.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2419..2814)
                     /locus_tag="ACG8ZM_002323"
     CDS             complement(2419..2814)
                     /locus_tag="ACG8ZM_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0493254.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2811..4223)
                     /gene="cysS"
                     /locus_tag="ACG8ZM_002324"
     CDS             complement(2811..4223)
                     /gene="cysS"
                     /locus_tag="ACG8ZM_002324"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0493255.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4220..4756)
                     /gene="epsC"
                     /locus_tag="ACG8ZM_002325"
     CDS             complement(4220..4756)
                     /gene="epsC"
                     /locus_tag="ACG8ZM_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0493256.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4842..5081)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5078..6538)
                     /gene="gltX"
                     /locus_tag="ACG8ZM_002326"
     CDS             complement(5078..6538)
                     /gene="gltX"
                     /locus_tag="ACG8ZM_002326"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0493257.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6708..7865)
                     /locus_tag="ACG8ZM_002327"
     CDS             complement(6708..7865)
                     /locus_tag="ACG8ZM_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0493258.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7899..9269)
                     /gene="radA"
                     /locus_tag="ACG8ZM_002328"
     CDS             complement(7899..9269)
                     /gene="radA"
                     /locus_tag="ACG8ZM_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0493259.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9332..9859)
                     /locus_tag="ACG8ZM_002329"
     CDS             complement(9332..9859)
                     /locus_tag="ACG8ZM_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0493260.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2506 a         2213 c         1784 g         3366 t
ORIGIN      
        1 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
       61 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
      121 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
      181 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
      241 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
      301 taaaattttc aagtttacca taaaaaaaaa gaaatgcaat aaaaataaat aagaagctca
      361 ttaagatttt tctaaattat tcctcattat aaaatttcaa gtgttttttt attttgagtt
      421 ttactctccc gtaagcgctc attacttgct tgtgatccat tgctaaacat tcagccatgt
      481 ctacaaaatc aacgccttgc atataataat gaaaaatttt agcttctatt tttgaaaact
      541 ggatttcgca attttcgatt gcttctttta ataaaagatg ctgaatcgct aaaggttctt
      601 gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc tattttttgg tcgattgaga
      661 cttcatcttt ttgatcacga cgcttcagcg cgttctgctt cctcaacaga gaatagattt
      721 gattgcttat aatgctttta taataacttc caaagctaac acctttgctt ttttcgtaag
      781 ttctcaatgc ctgatataaa gcgatccttg cttcttgttc ccaatcctca gaaacataat
      841 ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg aaaatatttt tgatacaggg
      901 cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa taactcctca gacataaaat
      961 aattcctcca taaattcgtt tatggaggaa tcttatcatc aatgattcat cgaactgatt
     1021 agaacgatat gttatttatt ttttctcatt aaatcgtcca atttttggga caattttttt
     1081 aactgctctt cattccatgg agaattacgt cggaaatcaa taaaatgcat atctttattt
     1141 tcttgggcta ttgttttttc cgtttttttt atcgtcttat ataattcata agcagaagtt
     1201 cgtaatgctc cttgggaaaa gatcatccac tgttctgcta aatcgcttgt cgccactgtc
     1261 acttgtgtca aacggtcatt cagctgacca gcgattctct cgatataact gtcagccgtt
     1321 tcgtcttctt tcgtaaaaac aacatccaac tggtatttct tataattttg ttggatgccc
     1381 ggaactagtt gggcatcgaa gacaacgatg acttccattc cttcatattt cgcataattt
     1441 gataaacgat gtaaaagagc ttctcttgca tccgccattt tgtcctgctt tttaagctgg
     1501 acaagttccg gccacgaacc aatcatgttg tagccgtcta caatcaacag ctgttttttc
     1561 atggtctact ccccctgacg ttgggaaaaa gcctgataca taagcaaacc agcagcaaca
     1621 ctggcattta agctctggac gtgcccggtc atcgggatcg tcagcaattc atccatttct
     1681 tttttcaatc ctggactcat tcctttgcct tcattaccaa tgactaaagc aattgatcct
     1741 ttggcattcc atttttggaa gtttgtgccg gtcatatctg tgccaaatac ccaaaatcct
     1801 gtttctttga gctgttgcac agcttgtacc aaattcgtca cgcgtgcaac agggacatgt
     1861 tcgactgccc cagtggaagt ctttgttacg ataggtgtga ttccaactgc tcggtgtttt
     1921 ggtatgatca caccatcgac gccacttgcg tctgctgtcc gtaaaatcga cccaaaattg
     1981 tgtggatctt cgatgctatc caaaatcaaa aagaaggggt cttctttttt ctcttttgtt
     2041 cgttcaatca gttggtcaag ggataaatat tcataaggtg taatcgcaag aacgactcct
     2101 tgatgcacgc cgtgatcgct caaagtttct aatttttgtt taggaaccca tttgactggc
     2161 acagcttgtt cacgtgcgat ttctttcagt tcttctactt tttctcctcg tgaatcttct
     2221 tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt ctactgcggc atggtgccca
     2281 aagacgaaat tatcttctaa tgcttcttca ttttgatcag aaactgactg atttcgtcca
     2341 gtttgctttt tatttgaata tctattttgt tttttgtctt caaatccacg tttcgattga
     2401 tgtttgtttg ttctttcttt acccattttt ttcaccaact ttctgaatac accagcggat
     2461 caattcttcc atccgctcgg tttgttttgt caaatgcaga tagcccatga gcgcttcgaa
     2521 ccctgttgcg acccgatacg tcgtaacatc agcatttttt gcgctcgtat ggcttttagc
     2581 atttcttcct ctgcgataat aaagttcttc ttcttctgtc aaaagatgtt cttccagcat
     2641 ttgctgaatc aaaaaggctt gtgcattcgc agaaacataa ttcgttgccg cacgatgcag
     2701 cttattgggt tttgtttctc cttgatcaat caggtggtca cgaacataga tttcgtaaat
     2761 cgcatctcca acataagcca aggctaaacc gttcaattgt gtgtaatctc tcatgcttct
     2821 cttctccatc gggttccttg cggtgtatct tctaataaga tccctttttc tttcaacaaa
     2881 tcacgaattt cgtcgcttcg gacaaaatca cgattgcttc ttgcttgatt tcgttcttcg
     2941 atcaattgtt caatctcttc atctaataac tcttctgctg taaataaaat accgaagatc
     3001 gaaagccatt gcgagaataa agcttctcct tgtgccaaga cagcggcaga aactttcggc
     3061 tggtctagat aagtgttcag ccacttagca agctcataga ccactgtgat cccatttgct
     3121 gcattaaaat catcgtccat ttcttcgatg aatcgctgtt ctaaattttc taacaaagcg
     3181 attttatccg catcatctgc caaattctct gaggcagttt cttggcggaa tttcaaattg
     3241 tcaaacgtat tcttcagtcg ttgtaaattg gtggctgctt ctttcatcgt cgtttcactg
     3301 tagcggatcg gacgacggta ttgtgtggtc gccatgaaga aacgcaagac ttgtggatca
     3361 acgtttttga tcatttcatg tacagtgatg aaattcccta gagatttact cattttttca
     3421 tcgtcttcgc caatcgttac atagccattg tgcatccagt aattagcgaa ttttttccct
     3481 gttttcgctt cactttgtgc gatttcattt tcatgatgag gaaattctaa atcttgcccg
     3541 ccaccatgga tatcgatcgt ttctcctaaa tgttttgttg ccataacgga acattcaata
     3601 tgccaaccgg gacgtccttt gccccatggt gaatcccaag aaatctcgcc aggttttgct
     3661 tgtttccata aagcgaaatc taatgggtct tctttttgct gctgttcttc ccctgtacgc
     3721 tgacttgctc cgacttccag ttcatcgatg gattggtcac ttaatttccc ataattttca
     3781 aatttacggg tacggtaata cacatcacct gaagattcat aagcatagcc tttttcaacc
     3841 aacacttgaa taaatgctaa aatatctggc atatgatcca tgactcttgg atgcagtgta
     3901 gcaggctcga cattcaacgc tttcgtatct tcttcaaatg ctcggatgaa acgatcagca
     3961 acttctggtg ctgtaatacc taattcgttt gctgtacgga tgattttatc gtctacatcc
     4021 gtgaaattcg atacgaaatc gactttatag cctcgatatt caaaatagcg tcgaattgta
     4081 tcgaaagcaa tggtgctacg agcatttccg atatggatat aattatacac agtcggtccg
     4141 cagacataca taccgacatg tcccttttgt atagggatga attcttcttt ttttctggtc
     4201 aatgtattgt agatttttat cattctgatc tcctcacttt ttctcctttg attcgaacga
     4261 cttttgctgg tactcctacc gctgtagcgt cgtctggaac atctttcaac acaacggcac
     4321 ctgcaccgat tttggcgttt tttccgatcg ttaccggtcc taatatctgc gcattcgcag
     4381 agagcatggc tccttgttta acagttgggt gtcttttacc cgtttctttt cctgtgccgc
     4441 ctaatgttac gccatggaat aaaatcacat cgtcctcgat ttctgcggtt tcgccaatga
     4501 cgattcccat tccatggtcg atgaacacgc cagttccaat cgttgctcct ggatggatct
     4561 caacaccagt taggaaacgc cagaattgag cattgatctt cgccaataaa aagagccggt
     4621 gctgatataa aaaatgagag aaacgatgcc aaaacaaggc atgaaaacca gggtatgtca
     4681 aaacgatctc agctgtcgaa cgtgcagccg gatcgtttct ctttgctgca tcaatcgctc
     4741 gggtaaacca actcatgact ttccactcct ttcgtaagaa ttttatgtag gaaatcatct
     4801 tcttataaag aaaagaagcg ccttttaaat aagtttcctt atcaaaagac gctttccgcg
     4861 tggttccact tttcttcata atcaatgatt atctctgaca gctaacgcct gttatacgtt
     4921 cttggctact ttgtttccta taaaacagtt tttcctgttt tctgtgtttc gccaagacca
     4981 ctcccagagg catttcggca accattcatt gataccttgc accacccggt atctctcttg
     5041 taatgatcag ctgcttactt ttttctgttc atcgtgttta gttcagtact tgatttaaat
     5101 gtgctaatga tttttcacga ccaagcaatt cgatcgtttc aggcaattct ggaccatgca
     5161 tttgtcctga tacagctaca cggattggca tgaaaagatt ttttcctttt acgccagttt
     5221 ctttttgaac agctttgatg gcagctttga cagttggagc atctactact tccatttgtt
     5281 ctagctgtgc tttgaatgcg tggagaacag taggaactgt ttcaccagca agtacttctt
     5341 ttgctgcttc attcaataca ggatgttcat tgaaaaatag cgtagacaga tcaacgattt
     5401 ctgcagcata gctcatttga ggttgataca ggctaacgat cttcttcacc cattcgattg
     5461 tcttttcatc tgggttttct gctacacgtc catcagcgat tagataaggc agacacatgt
     5521 cagtaaatgc atctagatcc attgctttga tgtaatggtt gtttacccat tgcagttttt
     5581 tcgcatcgaa agctgcgggt gatttactta ggcgttttgg atcgaacatt ttgatcaatt
     5641 cttggtgttc gaagatttcg tcttcgccca caggtgacca gcccaataat gcaataaagt
     5701 tgaacattgc atctggtaga tagcctaatt cgcgatattg ttcgataaat tgcaagattg
     5761 attcgtcacg tttgcttagt tttttacctg tttcactgtt gatgatcaat gtcatatgtc
     5821 cgaaggttgg tggtgtccag ccaaatgctt catagatcat cagttgtttt ggtgtattgg
     5881 caatatggtc atctccacgt aaaacgtgag tgattttcat caaatgatca tcgaccgcaa
     5941 cagcaaaatt ataagttggc ataccatcgc gtttttgtat gacaaagtcg ccaccgatat
     6001 tatcggattc gaacgaaata gcgcctttca ccatatctgt aaatgcgtat tctgtattgc
     6061 gaggcacacg gaaacgtaca acagattcta gaccttgtgc ttctttttct gcttgttctt
     6121 caggtgtcaa gttcgcacat gttcctgcgt aatgaggcat ttctcctctt gcgcgttgtg
     6181 cttcccgttc tgcttctaat tcttcttctg tgcaatagca tttgtatgct cgattgctag
     6241 caagcagctg atcaatcagc ggttgataga tttctttacg ttcagattga cggtaagggc
     6301 cgtattctcc aggattttca ggagattcat cccaattcat tcccaaccaa gcaaggtttt
     6361 ccaactggct tttttcgcca tcttcgatat tacgtttttg gtcagtatcc tcaatacgaa
     6421 taataaagtc tccatcattg tggcgggcaa acaaataatt gaataatgca gttcttgcat
     6481 ttccaatatg caggtgccca gttggacttg gtgcatagcg tacgcgtact ttcgtcattt
     6541 tcttcctctt ctttcaaact agactttgac taacagcaaa aattccgtca gttacggctt
     6601 ctcaaatatc atttatttaa agccactcag cacaaatttt accttttcta aggtaaatag
     6661 taaagccaac tggttgaatt tccttaccag ttggctgaat ttctttttta ttttgattgt
     6721 ttagtctttt tctctgtctc ttttttattg tcgattcctc gtcctgcatg tgcaggttta
     6781 gcaaagatca tgcgtccagc agctgtttga agggcacttg ttacaacgac ttcgatacgt
     6841 tcattcatgt aatgctgtcc atcttctacg acaaccatcg tgccgtcatc aaggtaggct
     6901 actccttgtt ggcgttctgt cccggctttt actactagaa cattcatcgt ttcaccaggg
     6961 atgacgactg gttttacggc atttgccaac gcattgatat taagaacagg tacattttgg
     7021 aattcagaga ctttattcaa gttatagtca ttggttacaa tgacaccatc taaaagctta
     7081 gccaatttga tcaatttgct gtctacttca ctgatatctt caaagtctcc atcgtacatt
     7141 tcgacagata taccctcttc tttttgcaag gcatttagga tatctaatcc gcgacgacca
     7201 cgcacacgtt tcaaactatc ccctgagtcc gcgatgtatt gtagttcgta aagaacaaaa
     7261 ttagggatca tcaaggttcc ttctaagaat ccagtctttg taatatcata aatccgaccg
     7321 tcaatgatca cgcttgtatc caagatttta tatttatgga aattttctcc tgattttcgt
     7381 tctaacagtt gactctcaac ttgactagct gtttcttgct cgattttttt gcttcgtgaa
     7441 ccaaaaactt tcttccattc ttcgattcgt gtcgttccca ttctcaatcc cagatagcct
     7501 aagatgatca tcagtaaaat cggcaataca ctattgacga atggaatttc catattgtaa
     7561 agaggggtcg agatcacaac acctaaagtc aatccgatga tcgcaccgat cgcaccgaag
     7621 agaagataag tcaagctgac ctcacttagt ttttgttcta cttgtttgat aatagctgag
     7681 atatgtttgg ccaacagcaa agataataaa aagaaaataa gtgcgcctaa caggctgttg
     7741 gtaaatgtat tattcagcca tccgtttgac tgttgaccga ttgcctgcca agctgagggt
     7801 aaaaatgaaa tacccaagct tgcgccgaca atgatcatca gcaaagtaat gacacgtttt
     7861 tgcatcgttt ttcctccgtt tcgacaatca tttattagtt agcggaacac acgcttcaac
     7921 gtttcagcca aagttgctac tccaactatc tcgattcctt ttggcgcttc ccagttacct
     7981 aagttgtttt taggtaggta tacttttgtg aagccgagtt tttggacttc tttgactcgt
     8041 tgttcaatat gactgacacg tctgatttct ccagtcagtc caatttcacc gatgaaacat
     8101 tccgttggct gtgttccttt ttctttatag cttgaagcaa tactgacagc gatagctaaa
     8161 tcaatcgctg gttcatttaa ttttacccca cctgcagctt ttaagtaagc atcttgattc
     8221 tgaagaagca acccagctct tttttccagc actgccatga tcagagatac ccgattgaaa
     8281 tcaagacctg ttgttgttcg tttggcattt ccgaacatag taggtgtgac taatgcttgg
     8341 atctctacca aaattggacg tgtcccttcc attgccacaa cgatagcaga gccagttgca
     8401 ccgtctaggc gttcttctaa gaaaatctgt gaaggattag cgacttcttc caatccatgc
     8461 tcatgcattt caaaaatccc aatctcattc gttgagccaa atcggttttt tactgctcgc
     8521 aaaatccgaa aactatgatg ttgttcacct tcaaaatata acaccgtatc caccatatgt
     8581 tctaacattc ttgggccggc gatcgagcct tctttggtaa catgaccgac gataaaaatg
     8641 gctatcccat ttgttttagc gattttgagt agttctgcag tggtttctct tacttggctg
     8701 acacttcctg ccacacttgt gatgtctggc tgcgtcatcg tttggatgga atcaatgata
     8761 acataatcag gggagatatg ttcgatggca cgactgattt cgttcatatc tgtttcagcg
     8821 tatagataaa attctgtgtt gatacttccg aggcgttccg cgcgtaattt tatctgctct
     8881 gcgctttctt cgccggatac atataagact tttccgccta tggctgctaa ttgctgagaa
     8941 acttgtaaca gcaaagtgga tttcccgata cctggatcgc caccaatcaa gaccatagac
     9001 cctggcacca cacctccgcc taacacacgg ttcagttctt ctaattttgt tttgatccga
     9061 ggttcttttt tagggacgac gtctgcgatt tttgttggtt ttgctttttc tcctgtcaaa
     9121 cttgtccgag ttctgcggtc cgttgtatct tgttcgattt cttcgaccat cgtattccat
     9181 tttccgcaat ttgggcagcg tcccaagaac ttaggcgaca catacccgca attttggcaa
     9241 acaaattgta cttttgattt ttttgccatt gtaccgcctc cttttatcct aatcatgaga
     9301 cattaaatca agcttaagta tctttaacgt tttatttact ggaagaacca aatccgcctt
     9361 gacgttgagc agtcgtttga tcttgatctg cttttaaaaa cggcaaaaag atcccttggc
     9421 cgatacgctc gccttttttg atcactacat ccttgaaacc aaagttggtg aattggaaca
     9481 tgatatggcc ttcatttgcc tcattattgt aataatcact gtcaatcaca cctactccgt
     9541 ttgtcaacac taaaaaccgc ttcaacggat tgcttgaacg gttagcaagc tgcaaaaatt
     9601 catcttctcc catgtaagct ttgattccag taggcacgag ggttggtttc aattgttttt
     9661 cattttctga tacatctttt ttcaatccat ctgcgactgt ttccaacccc tttttccagc
     9721 ctgctttcca gatacttgga atcacgacct cttctgctgc ttcaaaatca tagccagcag
     9781 catgatgtgt agcgcgctgc ggtagattga tattttgatc tttatattta gaaataactt
     9841 caaatcctct ttttctcacc ggttttcct
//
LOCUS       DAZKOR010000094         9667 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000094 DAZKOR010000000
VERSION     DAZKOR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.096"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..632)
                     /locus_tag="ACG8ZM_002330"
     CDS             complement(<1..632)
                     /locus_tag="ACG8ZM_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0493261.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="ACG8ZM_002331"
     CDS             975..1391
                     /locus_tag="ACG8ZM_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0493262.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="ACG8ZM_002332"
     CDS             1388..1858
                     /locus_tag="ACG8ZM_002332"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0493263.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="ACG8ZM_002333"
     CDS             1872..2645
                     /locus_tag="ACG8ZM_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0493264.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="ACG8ZM_002334"
     CDS             2659..3471
                     /locus_tag="ACG8ZM_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0493265.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="ACG8ZM_002335"
     CDS             3525..4490
                     /locus_tag="ACG8ZM_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0493266.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="ACG8ZM_002336"
     CDS             4606..7329
                     /locus_tag="ACG8ZM_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0493267.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="ACG8ZM_002337"
     CDS             7342..7974
                     /locus_tag="ACG8ZM_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0493268.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9667
                     /locus_tag="ACG8ZM_002338"
     CDS             8873..>9667
                     /locus_tag="ACG8ZM_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493269.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLYL"
BASE COUNT         3261 a         1477 c         1884 g         3045 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtattta
//
LOCUS       DAZKOR010000095         9603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000095 DAZKOR010000000
VERSION     DAZKOR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.097"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..467
                     /locus_tag="ACG8ZM_002339"
     CDS             <1..467
                     /locus_tag="ACG8ZM_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0493270.1"
                     /translation="KTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFV
                     SVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPK
                     FMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            476..703
                     /locus_tag="ACG8ZM_002340"
     CDS             476..703
                     /locus_tag="ACG8ZM_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493271.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            907..1986
                     /locus_tag="ACG8ZM_002341"
     CDS             907..1986
                     /locus_tag="ACG8ZM_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0493272.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            2728..3099
                     /locus_tag="ACG8ZM_002342"
     CDS             2728..3099
                     /locus_tag="ACG8ZM_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493273.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(3355..3597)
                     /locus_tag="ACG8ZM_002343"
     CDS             complement(3355..3597)
                     /locus_tag="ACG8ZM_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0493274.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3629..4531)
                     /locus_tag="ACG8ZM_002344"
     CDS             complement(3629..4531)
                     /locus_tag="ACG8ZM_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0493275.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4544..4732)
                     /locus_tag="ACG8ZM_002345"
     CDS             complement(4544..4732)
                     /locus_tag="ACG8ZM_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0493276.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4746..5309)
                     /gene="amaP"
                     /locus_tag="ACG8ZM_002346"
     CDS             complement(4746..5309)
                     /gene="amaP"
                     /locus_tag="ACG8ZM_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0493277.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(5347..6240)
                     /locus_tag="ACG8ZM_002347"
     CDS             complement(5347..6240)
                     /locus_tag="ACG8ZM_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0493278.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(6318..7241)
                     /locus_tag="ACG8ZM_002348"
     CDS             complement(6318..7241)
                     /locus_tag="ACG8ZM_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0493279.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(7290..7640)
                     /locus_tag="ACG8ZM_002349"
     CDS             complement(7290..7640)
                     /locus_tag="ACG8ZM_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0493280.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7673..8575)
                     /locus_tag="ACG8ZM_002350"
     CDS             complement(7673..8575)
                     /locus_tag="ACG8ZM_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0493281.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8568..9425)
                     /locus_tag="ACG8ZM_002351"
     CDS             complement(8568..9425)
                     /locus_tag="ACG8ZM_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0493282.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2621 a         1949 c         1768 g         3265 t
ORIGIN      
        1 ttaaaacatt gctattaaaa tttgtcgttt taggtataag tgccgtattt attttgttcg
       61 gcgtactttt ttatactcag atcgctacaa gcgaaaaagg attcaatttt gattggcaga
      121 ccacattatt tgtttctgtc atttactttg cccttctcct agggatcgtt attgcttatt
      181 tcttattgaa actattgaaa aaaatcgaag ataaccaagc tttttccact caaagcttaa
      241 acatactaaa aaaaatcgaa cgaaccattt tgtttattgg tttcgccttt tttggcttac
      301 ttcctaaatt tatgaaagta gcggatttag acgatgctcc aggtttgatg ttggtaggaa
      361 tcgctgtcgt attcattcct tttgcagtat acacattaac aagcgtgctg gaaaaactgc
      421 ttgaacatgc gattttactt aagacagatc atgatttgac agtgtgaggt gcgacatggc
      481 aattatcgtt aatttagatg tagtattagc tcaaaaaaaa atgagtgtga ctgaattgag
      541 tgagaaagtg ggcattacga tggcaaacat ctctattcta aaaaatggaa aagcaaaagc
      601 catccgtttt tccactctcg aaaaaatctg ccaagttttg gagtgtcaac caggtgattt
      661 gttgacctat aaaaatgact cgaatactat accagaagaa taaaataaag tagaacaaac
      721 agtaaataaa aaataatctg aatgatagat gtgtgagact tttagaagtt atttacgtct
      781 attattcaga ttttttattt ggcggatttt ataattaact tagccatttg gaaataaaaa
      841 aacgccacgt gaattccatg ttatattgaa gttaccacaa ctctcaatag acaaggatga
      901 attcacatga cgcaaaacca tgataacaca gtttcacgca agggaaaaca cctaacctac
      961 tcagaacgtt gccaaatcgc catcctgaaa aaggaaaact attccaaccg tcaggttgcg
     1021 gatgtcctgg gctgcgtgcc gcagaccata aacaacgaaa tcaaacgcgg gaccgttact
     1081 cagcttaacc gccagaatca gaacgggaag gtctacgact attatcatga cgtctacgac
     1141 gccgatgccg ggcaggcggc ctacgagaag aaccgcttga actgcggcag acggccctta
     1201 tgggccgaca cggacgcctt cattgaatgg gcggacgaca tgatgctgtt ggaaaaatgg
     1261 tccccggacg tggtggtggg ctttgccaga aaacacgaat tgttcgaccc ggccattatc
     1321 cctgggacca ccaccctcta tcagtggatc gaccgtggca tcatgaaaac gaagaatatg
     1381 gacctgctag aaaaactgtc ccgcaaaccg aaatcgtccc gtcatggcaa ccgcccgaat
     1441 aaacgggtgc tagggaaatc cattgaagag cgcccggagg cggttggtac ccggaaaacc
     1501 ttcggccatt gggaagtcga cacggtcgta ggaaacaaga ccaagtcgga tgccgtgctg
     1561 ctgacgttgg tggaacggca gacacgcttc gaggtcattc tgaagctggc cgggaaggat
     1621 gccgaatcgg tcaaccaagc ggtccgacag ctgagagaac gggctggtgc gtacttcggc
     1681 cagctgttca agacggttac ctcggacaac ggctcggagt tcgcgggact gcacgatgcc
     1741 ttgcaggatg tggcaaacat ctacttcagc cacccctatg cttcctggga gcgcgggacg
     1801 agcgaaaacc aacacaagct gattcgccgt ttcatcccca agggcagccc aatcggcaac
     1861 gtgagcgaaa accaaatctt gcggattcag cgttggatga acgattaccc gcgaaagatt
     1921 ttgggctatg caacgccgca tgacacgttc gtccaggcat ttaggcagga acggttgacg
     1981 gcttgacacg tgggcccttg acaacgagcc tccctcggca tggcttgtgt ctggaaagct
     2041 ggccggggcc agccagtcgg gagactacca tgcctcagct cattgtcaag ggcccactcg
     2101 attagaaaaa ttagacggtt ggtggtaacg agcaaccggg tcaccatggg tggctaactt
     2161 aaacttgaaa tttacgagat tttttatttc gattgaatac cgttttatct ataaataaga
     2221 tatgtactga ctcatacaaa gggggccttt tcttctccat gtactatcac ttttcctgat
     2281 acaactggtt tcctgttacg ttgtagttgg atctgctttt agtataatac tttattcatt
     2341 ccaccagact gatagcagtt tgagcagtct aaataatcat gtagtattac aggccattct
     2401 gtttcatacc ttgaagtgct taaaacttca gctaatggcc cattgtgtta actcttactc
     2461 cattcttttt tagtaagctc ccatatatac tgtaaagcct tctatatatt atgtcatgtt
     2521 gctttttaac atttactatg tagttttgat aaaatgattt gaataacagt gatggacaat
     2581 tcttttttat ttttaacatt attttgataa taatgattat ttatttaaaa atacattaga
     2641 taacatttgt tacgacacga aaaagcttca caaacaaata tcgttatttt aaaatgaggg
     2701 cgaactcaca caattaggag gaatagtatg ccatttattg tttacttttt tctttcatta
     2761 ctcgtgatct atattccatt accaacattt ttcctgctcg tcaatcgtct agcagagcaa
     2821 catgatacga ttgctgctct tacgaaactt tgtttacttc tatttgtttg tctagattac
     2881 tttacgagct attattggct aaaacagaca agagtaaaaa agagatatta tttcttcctt
     2941 gtctttttca atctctttga gtttctgatc cattgctttt tacttcatca gtatcatacg
     3001 atccatttgt ggatcatctg ggttttcaaa gtggtcgtat tattttgtat gttcaccttt
     3061 cctatctcaa gaaaatttag ggaggcattg ttttattaaa gaagcttctc tgtatcaata
     3121 aagacacatt taaggaaaaa cttttaaaaa tctaattggg aatttgcccg cataaataag
     3181 aggctgtgac aaaagtcttg tcacagcctc ttattccgta taaacagtgt tcaaaagctt
     3241 gcatcttcga taatatgccc aaccaaatga aaaatatgag aagctatttt tgtttggctg
     3301 ttcttattac ttgatgaaga acagcttttt cacgacctct ttttaaaagt ctttttacag
     3361 ttttcgagca aagaatgata cgattagtac taaaatgatg gcaccaatca atgatggcac
     3421 gattgccatt cctgcaatac ttggtcccca agaacctaat aatgcttgac caagtgctga
     3481 tccaactaaa cctgctacga catttgcaat aatccccatc gaagaacctt tattcgtgat
     3541 tgctcctgca attgctccga taattcctcc gacgataagt gaccaaataa atcccatgtt
     3601 tcgttcctcc taatgtttta cttccttatt actgaacccg tggttctgta ttttctttta
     3661 ctttttgtgc gccgcttcca gctgctgatt ttactttatc tgtttgattc gaagcaaatt
     3721 caccggtttt tttcgctgcg tcagttactt tgtcttgcac cgtttcttga tctttttcaa
     3781 attcttcttt tgttttgatg tccactacgt tagcgttgac ttcgatcact tctaaatgtg
     3841 tcatattttt tacttctttg ccgattactt cttgcatttc ttcaaagatt ttttcgatat
     3901 ctttgccata ttccacaatg acatccatat caactgctac ttgtttttta ccgacttctg
     3961 tttcgatccc agccgttaca ttatctgtat tcacaagttt tcctgccaca ttagagaaga
     4021 aaccaccgtt tgcagataat aaaccgtcta cttgttcaat tgcgattccg atgatttttt
     4081 gcacaacttt atcttcataa ttcagctcac cgcgcattga atcttcctgt tcagaatcat
     4141 gctttccttg gttatttgta tgatcagaac caagttttcc ttgtccgttt gtgtggtcag
     4201 aactaagttt ttcttgtgct ttcgcatggt cagaaggatg tttttgctct tccacaggtt
     4261 tagaaggatc gattgcatca tgttgttctg gaactttaga ttctggagtt cctggatgat
     4321 gaggtgcatg cttttctgca tcatgtttct tctcttctac tccttgttga acgttaggga
     4381 ctactggatc tggatgtgca gatactggtg ctgttttgtt cttttcatcg ttcacaccat
     4441 ttgattggtt tggtgtcgtt gggtctggat gtgcagacac tggtttttgg ttgttcattt
     4501 tattgttatt atttttgtta ttgttattca tggatgtttc ctcctaagat ttaaaatagt
     4561 gatctaagat tttcgtttgt ttcagataaa aaccgatagc gacgccgatc gctgccatga
     4621 tcaagatcaa taaggtcttg aaaaagccca cggtgatcca taaaacggct aatatcaatc
     4681 caagtacccc aaagatgata gggagcttgt atgtcgaaaa tagttctttc atttttggtc
     4741 cccccctatt ctactcgttt gcggtcattg cttcgtttag ctggttcttc aaagcctgtg
     4801 tatttcaccg atactttcac tggctctttt gttcccaata tgtgttgtac ttcattttcg
     4861 atttctttca tcaatgttcc tgttttacca atgagtgctg aagttcgttt caactcccct
     4921 tttacattga cggcgatctt gtttttcgtt gctttgacat ttacttttgg tgaatggatg
     4981 aattcatctt catgcaagtg agaacgaacc attccttcaa ttgctttttt atccaacgtc
     5041 aactccccat caccatgttt cagtagaaag tttccgattg tttttggata aaacaggaaa
     5101 acaagtaacg tgataattgt caaaatagca aatgcaacgg caagccaaaa tacatattgt
     5161 tgcacgtatg aattagtgat ggaaaagaat cgaaacttct ctagctgaaa aggcaatgga
     5221 taggttgcac ttaatagtat cgcaaacaaa agcacagata gcaaaagtaa tgttccgatc
     5281 actccgatcg tttttacacc tttattcatt ttcgcacctc ctcatagggg tgaaacgatc
     5341 aacttcttat aagttgatcg cttctccacc agttactccg taaatttggg ctgtaacata
     5401 acttgcttta ttcgatgcta agaaaacata aactggtgct aattctcctg gctgacctgc
     5461 acgttctagt aaactttgct gaccaaattc agggatcgaa tcagaaggtt gtccttcgtc
     5521 tagttgtaat ggtgtccaga ttggaccagg tgctacacca tttacacgga ttcctttagg
     5581 tgcaagctgt tttgctaaac cgatcgtaaa gttagcgatt gaagcttttg ttgctgcgta
     5641 atctaataaa tgttcgcttg gattgaatgc ttgaacagaa gttgttgtta cgattgctcc
     5701 tcctgctggg atatgcggaa cagcttcttt cactaaagca aacatagcga tgatgtttat
     5761 cataaatgtg tctttgactt gctcaagtgg taactcagca atagactgac aagaaatttg
     5821 ctgtcctgcg tttagtacca tcgtatctag cccgccaagt tcactcacgg ctttatctat
     5881 gatttcttta ggtgcttgtt cgtcacgaag atcaaaaggt agtaagacaa tcttacgtcc
     5941 tgcatctttc acgtattttg ctacttcctc tgcatctttt tcttctcctg ggaagaattg
     6001 aagagcgata tctgcccctt ctctagcaaa tgcaatcgct gctgcacgac cgatccctga
     6061 atctccacca gtgattagta cacggcggtt ttctagttga ttattgcctt cataggattc
     6121 ttctccacaa tcaggaacag gaatcatatt ctgctgtaaa gctggtgtat cctgatcttg
     6181 tttaggaaat ttctccgtat ggtacaagtt tcgcgggtcg attaattttg gttctgtcat
     6241 tgctgtattc ctcctagttt ttaaattgta ttgccaaaac aaatttcttt gtttgatacc
     6301 tgttttaaat agattcttta atttgtataa tctttccggt ataaatggta acctaaaact
     6361 actgctgcta tcaaagagcc aaagactacg agtataaagc ctagagcaga acttttaccc
     6421 ggtaatcctc ctgtattcat tccccaaatc cctgcgaaca acgccggaac ggaaatgatt
     6481 aatgcagctg aatctagata tttcatgata tgatttagat tattgtccat catcccgttg
     6541 aatagatcac caatactctc taataaatct cgatagatct cgatcatttt ttctgtttgt
     6601 cgctgtctca aatggacatc atagagtagc tgttcatcgt ctaaacgttt gatgaaagtt
     6661 tcagaattcc ataattgatc cattgtttcc cgttgatctc ttaaggtatg atcgatatat
     6721 acgatgtctc tttcaagatc tgcctgttta tacagttctt cattttccgt tgttttacga
     6781 gctgctttat ccaaagcgtc aattcgcttt ttcatctctc ttagttcttt cacgtaatgg
     6841 gtgtaaatat ctaataatgt atacgtgatg atcttttcaa acgagtttaa atgatttcca
     6901 tgctttgtca gcagccgatc cacaaattcg gtttttctat cgatacaagt aatcaagaga
     6961 ttatcggaaa gtacaaaaga tattggcgtt aatttttttt cgatacgatt atctgactga
     7021 tctagattaa tcactaccaa agaaaatgga tgctctagtt tcgtctcata aaggcgctcc
     7081 atccgagaaa cttcttccgg atattcggtt cctacgaaaa tatcatcagg aagctcgaat
     7141 tcttcgattg cttgctcaat ctcttcgggc tttgcactat caagtacgag ccaattatat
     7201 tttttttcat ctgtttctat tacattcttt tcttcatcca caaagtagta tctcaatgta
     7261 tacctccttt ttggactttc tcgaaactat tagctgaaga agcagttctt taatttgata
     7321 ttataatcga gtactgcgat ttcattttct aatgaaaagc tgtcaagctg tttgttgaag
     7381 tccatgttca atcgtttacc gaaataattt tgctctaatt tagatgtata gatgtccaat
     7441 aatggatgct cgattttagt cgcatattct ccaaattccg aaacaaaagg aatgatttga
     7501 aaaaagctgc cttttgcaaa agcacctttg tcccttagaa atggatcgga atcattgata
     7561 agcgcgagtg cgatttttgt attttcccca accttttcaa ttaacgtctc tactgcattt
     7621 ttagacattg taatttccat tcctactccc cctcttcagt tgtggtcttc tattatttaa
     7681 caactacatc tgtttttggt tgaacttttg catattttgg atatttaggg aatttagcta
     7741 cacaacggaa agccgtcatc cctgcatatt tttcgtaata tttcttcgtt ggcccttcaa
     7801 aatccttgct ccacggtttc acatgtcctg aaaaatggat aatgtaagga ttgttgcgag
     7861 tttcttcgta ttcacgttca cctgctgctg ttggatgttt ttttgctttt gccatgatat
     7921 agccttgtgc attccaacgc ggatgaagtg gtaaccaacg gtcatgtaag atagcattta
     7981 atgcatcttg gtcatggaat cgtaattttt ctggattatg ttcgatgaaa tctaatactt
     8041 tttgcgtaat attttcatct aaccattttt tcacattgat cagcatcaaa cctgagttga
     8101 aataacgcat tgatttagct gaaattttca ttttttccag tcgttgatgg aacccagcgt
     8161 cttcaacagc tgctacgatt gaatcaccga agtcaagacg ccataattta gagatatctt
     8221 gtaaagcgat catgtcgcaa tccatatata agatacgttc aacttctgtg ccgcggaaca
     8281 attccggaat cgcaatacga taataagctg tttctggaat acgatcgctg attaccacat
     8341 ttgtaaagaa ttctttattg attttcaaga attccacatc tgagttcatt cgtgcgtttt
     8401 tcactgagaa gcgtaaaagc tgtttacttt caaaatcgat atcatcgtca atcacataga
     8461 accgaacaaa tttagacggg ttgcaattat ctaatactga gacaaataat gcagctaaat
     8521 gtggtacgaa ttttgtgtta caacttgcga ctactgctat ttctttttta ttcattgatc
     8581 tcgcctactt tcgttagagt actgtgtgct aattttttta agtataaatt ggtatacgga
     8641 tgatctttca acgtattcca aggtttatca tgtcctgtaa agtgaacgat cgaaggtttt
     8701 tcatttcctt ctttatactg acgttcatat ttttcatttg gtgctggatg acgttcaaaa
     8761 atcagcgaag tctgcatgtt ccattttgga tgaagttgtt cccaatcttc ataaagtaca
     8821 gcattcaaag catcttggtc gtgatagatg attcgatcgc cattttcact gaggtaatgg
     8881 atagtttttt ctgtaatttc ttttttattc cattgatcaa tgtcaatcac catcactcct
     8941 gaattgaagt acaagtcatc ggaatcgatt cctaatcttt ccaaagcttt cacttggcct
     9001 ggatcgataa tagcgccgat tgtttttcca tttaacggct catcatatag ttttacgata
     9061 tcgtctagta ctaacacatc tgaatctaag taaaggactt tcttataatc ctctttagct
     9121 aataagtttg gtaaagaaat tcttaagtaa gcggtagttg tgatatggtc actgactagg
     9181 aagtcttcgt agatatcttt ttccactgtc aggaattgga tacttgcatt acttgaatat
     9241 ttatttaccg tttcttccaa tccttcttta ctatattctg atagaccatc gtcaatcaca
     9301 taaaatttta tgcgtcttgt tttattgcaa ttttctaatg cagttgcgat catcacgctc
     9361 aagtaaggag cataattttc gtctgaagca gttaccacag gaactacgcc gtatctcttc
     9421 tccatcaaaa tcactccttt ttctaatctt tttctaacct gttctacatc tagaatattc
     9481 ctcatttggt tttaaagcaa aatatatgct ttgttttcat tgaatatttt aaaatttttt
     9541 tatcacaaaa cgcatcggta agcgctaact attcgttttt catggctttt tattcaaagg
     9601 tat
//
LOCUS       DAZKOR010000096         9590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000096 DAZKOR010000000
VERSION     DAZKOR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.098"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..154
                     /locus_tag="ACG8ZM_002352"
     CDS             <1..154
                     /locus_tag="ACG8ZM_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0493283.1"
                     /translation="NKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKV
                     LLGIQA"
     gene            206..352
                     /locus_tag="ACG8ZM_002353"
                     /pseudo
     CDS             206..352
                     /locus_tag="ACG8ZM_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            429..758
                     /locus_tag="ACG8ZM_002354"
     CDS             429..758
                     /locus_tag="ACG8ZM_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0493284.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            748..1035
                     /locus_tag="ACG8ZM_002355"
     CDS             748..1035
                     /locus_tag="ACG8ZM_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0493285.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1321..2178
                     /locus_tag="ACG8ZM_002356"
     CDS             1321..2178
                     /locus_tag="ACG8ZM_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0493286.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            2179..2727
                     /locus_tag="ACG8ZM_002357"
     CDS             2179..2727
                     /locus_tag="ACG8ZM_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493287.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2987..3448
                     /locus_tag="ACG8ZM_002358"
     CDS             2987..3448
                     /locus_tag="ACG8ZM_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermophilic serine proteinase"
                     /protein_id="HFQ0493288.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNR"
     gene            3492..3578
                     /locus_tag="ACG8ZM_002359"
                     /pseudo
     CDS             3492..3578
                     /locus_tag="ACG8ZM_002359"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(3544..4614)
                     /locus_tag="ACG8ZM_002360"
     CDS             complement(3544..4614)
                     /locus_tag="ACG8ZM_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0493289.1"
                     /translation="MKNDIVTYANTKCEEIGISNLEECFFDTNDLSQFLRTGRTNASK
                     LANDLVAKNVLFKINTRPVLFAIKERFEEHFLIPVKDVYESISELKTDLEPNSTDSIL
                     QKMIGYDMSLSDAVEQMKTAVFYPGRGLPMMITGDTGVGKSFFVHTAYQFMKKVNVIH
                     ENAPFKVLNCAQYHNNPELLSGILFGYVKGAFTGADEDRNGLLQDADGGVLFLDEVHR
                     LPPEGQEKLFVYMDGGTYSRVGETIHHASNVRLVFATTEGKEDFLETFMRRIPIHIHI
                     PNIEERGILEKNKLSKILSLAKAKTLARLLMFLLKSLVLHKYSQNILNYYFLEKSKKN
                     RIPNFHLHFQKDLHVDNQFLNQ"
     gene            5216..7060
                     /locus_tag="ACG8ZM_002361"
     CDS             5216..7060
                     /locus_tag="ACG8ZM_002361"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0493290.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            7116..8582
                     /locus_tag="ACG8ZM_002362"
     CDS             7116..8582
                     /locus_tag="ACG8ZM_002362"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016620473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0493291.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            8619..9461
                     /locus_tag="ACG8ZM_002363"
     CDS             8619..9461
                     /locus_tag="ACG8ZM_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0493292.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         3294 a         1473 c         1936 g         2887 t
ORIGIN      
        1 caataaattc tatcgaagtt tacgcactgc ctctaccacg attaaaggca tggaagccat
       61 tcgaggatta tataagaaaa cccgaaaaga aggcactctc ttcgggtttt cggtctgtac
      121 tgaaatcaag gtattattgg gaatccaagc ttaaatcata gataccgtaa gggattttat
      181 tctttattta aaactttgca acagaaccca atcatttgag aagttaaaac ctacacatga
      241 aggtaggtat tttagaagat tgaatagaca acaccgggtt gtttataagg tagatgaaga
      301 tgaacacgtg gttgaaattt attcagcaag gacgcattac gagtcacagt aattacttgt
      361 aaatacatta tctcgtggta taatgtattt actaagtaat tacatactgt tagaaatgag
      421 ggatttaaat gattacaaaa acaagaaaac aaggcaattc aatcatgttg acagtaccta
      481 aagattttaa tgttcctaat ggagtcgaag ttgaagcaaa gttggtagag aatggtattc
      541 tttatgaatt tgttgaacca caaaaagaat tttatgattt tagcgaagat attttgtctg
      601 atataattgc agaaggctac gataaagatg agattcttgt agaatttaag aatagaaaaa
      661 acaaaatgca ttcttccttt agagatattg ctgaagatac acttactaat tctaaagtaa
      721 tgactaaaga ggagttggca acagaaattg gcttatgaaa tcgttccttc aaaacatgtc
      781 attaagtatt taaaaaagct aaaagagaag ccattgaaag aaaaattttt gaatttaatt
      841 tatgatgaaa ttgcgattga tccatataag ggtgaacaaa aaactgggga tttatcaggt
      901 atttggtcta gtgggttcaa atatgcgggc acaacatata gagtagctta cgagattata
      961 gaaaacaagg ttattccagt attgttatgt ggaacgcatg aaaactttta tgaacagttg
     1021 aaaaaattaa tctagtgatt tgttgtcgtt cttaagaagg acggcttctt ttttatcgaa
     1081 aatctgattc gtctacatga ctaaagtcgc gagtattctc tgcttctcga taaattaaat
     1141 aacgtcatta ctttgaggaa agctataaat caatatattt cagcttttcc ttatttatgt
     1201 actacctttt acttattaat tataaggtag tacatatata tctttttttt ggtagtaact
     1261 aaaaataaat atgttataat agtatcaaat gaatattgct aaaagaaaag aggtaggaac
     1321 gtggtagcga agaaatatgt aatcggtaat tttaaaggtg gcgtaggtaa aagtacgtgc
     1381 gcgcaaatgt tagggtttga atcagcagtt aatgaaggaa aaagaacctt gataattgac
     1441 ttggatatgc aaggaaatac ttctgatgtg atgaacttaa cgcatatgaa cttcactaac
     1501 gaagaaggag gcggagaagg tgaaccgatt gtttacgaaa atactgtgac tgacgtgttg
     1561 ataaacaatc ttccacctaa agaggctgtc tataaagtaa tcaataacct ctatatactt
     1621 ccagcagaca tgtcctttga aatgtatgat gattggatca aagaccaata taatgattca
     1681 ttatcccaat tcaaatatat gaaagaaaag ctagaaccat tatttgatga gtttgacgtc
     1741 atctatttag atgtcccacc ttctatttct gtatatagta aatccgcgat gtacattgcg
     1801 gattgggcaa tcgtcgtctt acagacccaa gtaaaatcga tgagaaatgc gatgcaatat
     1861 cttgagtata tggacttttt tgtgaaagaa tttgatacaa atttatatgt tgctggtgtt
     1921 attcctttca tgttggaaag cggtgacgca gtagatcagg aaatgtataa acaagcccaa
     1981 gagatttatg gggaacattt gattaaaaat gttgtactca aaaatgcgcg cttaaaacga
     2041 tacgacggat caggtattac gatggagaag acaaagaaag gtctcttaaa acaatgggat
     2101 cgcaaaagcc acgaattatt tatcagtatt ttgaaagaat tggaagaaca cgagaattgg
     2161 tatgtggagg tggagtaatt ggctgacatc aaaggacttg atatttttgg gaacaacaaa
     2221 ggactcgcca aaataaaaaa agaagcagaa gcaaagaaaa agcagcaagc aattacacca
     2281 tctgaaaata agcaattaga agtgcaccaa gttcaagaaa taaaaacttc ctccaatgtt
     2341 gaaagacaga gtagaggaag aacaaaaaaa gttggcgcac caatcagaaa gtttgataag
     2401 gtttattcca ccaagcatcc tttaaaactg tcgactttgt taaatgcgac ttccagagtt
     2461 ttagtagaaa agtatgagac taatatgaca agagatgaat tgcttagaaa agctttagat
     2521 gagtatatta aacagaatct tacgcaagaa gataaacaag atttgtttaa tgacgtggtg
     2581 aaagaactag acttgttcag agaaaaatat ccaattgttc cagaattaga tgatcgtgga
     2641 gaaataataa gaactaccag tcagataaaa caagataccg aagactcact gcgcaaaggg
     2701 tggggaatga ataataaagt tagataataa tcaaagaaga actaagtaac tacctttaaa
     2761 cggtagttac ttagttcttc tttgatttgc gtgaaataga ggggatttat ttttctggtt
     2821 acgtgattaa aaaatccaaa aaatgagcat aggaacggtt atgattttag ggtgtgtttt
     2881 aatgagatat tgtgatttca ttaataagtg tatgataatc tgttgttgag ttagtaaata
     2941 agtaatagag ctaactctaa aataaaacta ttaggaggag atgtagatga aaaagtttaa
     3001 aaagaaaata tatttaggat tggttgttgg ggtgagtatg tttgggttca tggctcatac
     3061 taaacaagta actgcagata ctaaccaaaa agaaaggatt atgagccaat tagatgactc
     3121 tcaaatagag tatgtaccca atgagctaat tgtcagttta aaggctacac aaagaatttc
     3181 aacagagaaa aataaaccgc aaaatttatt gggtgtaaaa gttatggtgg aaaaagattt
     3241 gactcttagg acaaatgaaa gcgattataa taagcgagat caagtattat tagtgaaatt
     3301 gaacgaagaa attagtgtgg aagaggcagt aagaacattt aatgcttctg atatggtcaa
     3361 ttatgcagag ccaaactatg tggttgaact agagccgttg aatgatgttg aggagccaaa
     3421 tacaactgat tctttaggaa atcgataacg atcctaattg gagtaaacaa gctaatttaa
     3481 gagagattag catgccatcc gcttgggaag aaaccaaggg ttctaaggat gtgaaggtag
     3541 cggtcattga ttcaggaatt gattatcaac atgaagatct ttctggaaat gtagatgaaa
     3601 gtttgggata cgattttttt tacttttttc aagaaaataa taattcagaa tattctgact
     3661 atatttatgc aacactagtg actttaggag aaacatcaat agccttgcta aagtttttgc
     3721 tttcgctaaa gagagtattt tcgataattt gtttttttct aaaatacctc gttcctcaat
     3781 atttgggata tggatatgta tagggattct tcgcataaag gtttccagaa aatcctcttt
     3841 gccttctgta gtcgcaaaaa ctaaacgtac attggaggcg tgatggatag tttcaccgac
     3901 tctagaataa gttccgccat ccatataaac aaatagtttc tcttgccctt caggaggcaa
     3961 tctgtgtact tcgtctaaaa acagtactcc accgtctgca tcttgcaaca atccatttcg
     4021 atcttcatct gcgccggtaa aagcaccttt aacatagcca aatagtatcc ccgacaataa
     4081 ttcaggatta ttatggtatt gcgcacaatt aagtacttta aaaggagcat tctcatgaat
     4141 aacgttcact tttttcataa actgataagc agtatgaaca aaaaaacttt ttccaactcc
     4201 agtatctcca gtgatcatca taggaagccc tcttccagga tagaagacag cagttttcat
     4261 ttgttccacc gcatcggata agctcatatc atacccaatc atcttttgta gaatggaatc
     4321 tgtagaattg ggctccaagt ctgtttttaa ttcagatata ctttcgtata catcttttac
     4381 cggaatcaaa aagtgctctt caaagcgctc tttgatggca aataacactg gacgagtatt
     4441 gattttaaat agaacgttct ttgcaactaa gtcattggca agtttactgg catttgttct
     4501 tcccgttctt aaaaattgcg acaggtcatt tgtgtcaaaa aagcactctt ctaaattact
     4561 gatgccaatt tcttcacatt ttgtgtttgc ataagtcact atatcatttt tcatcttata
     4621 aacagtcctt tctaaacttc tatcccttaa ataaatagca aaaaaaagga agcaatatcg
     4681 atatgctgtc ctcttaattt aataaagcgc taatctaatt cactgtatta tcatttttta
     4741 gtagtatagc acaattatga atgccttttc tttatatcaa ataaagttta tataaaaaac
     4801 ctcatcctgc aagaaagaaa aaatgaaccc attcttttta tactaggtct taaaaagaca
     4861 gaacatctgc tagaagatag aagagaataa gagttatata tgttggaaga tattcttaga
     4921 aaaaagacaa aacttttaga acacgaaaac tatattgaac gaataaaatg aaaaaatgat
     4981 taaaaaatca ttttattatt tacgtttaca ttctaatatg ttaaattgaa tataacttaa
     5041 acaggcatgt gactggtaat gcaggcagga cctaaataag ttgaataaca atgtatgcgt
     5101 ttcaatacac gtttgccttg ttgtcaattt atttagtttc tgcctgtttt ttgtgttgag
     5161 gatggccact tcgactcaaa agttcatcac tcaaaaaaga aaagagggaa acaccatgaa
     5221 ttacaaacaa acagcagaag aagtattgca gtacatcggt ggggaagaaa acatatccca
     5281 tttggagcat tgctcaactc gtttacgttt tacgttaaaa gacaacagca aggtaaatat
     5341 gagccagtta gaaaaagtgg atggcgttat gggcgttcgc caaaatgtgc aatgccaagt
     5401 aatcattgga aacgatgttg ttgaagtgta tgatgaagtg aaagcgttat taggtgacaa
     5461 tgtttcaaat actgaagaag caccaaaaca aaaacaaaaa ttaggttcag ttctattaga
     5521 ttttattatt tctattttcc aacctttaat tcctgcgatt gcaggtggag gagtattgaa
     5581 gtccttgttg ctactagctt ctatcttagg gctgattagt gaccaatctc aaacctatca
     5641 gattttaaat ttggtcggcg gggcaccgct ttatttcttg cctattctgg tggcaatgac
     5701 tacggctaat aaattgaaag tcaatcaatt ggttgcagta tctgctgtcg gtgcattgat
     5761 ccttcctgaa atgacagcta tgttggcgga aggtgctaca ttcttatctt ttggattgga
     5821 taatatagcg tatgcatctc aagtattccc agccatctta actgttttat tttatgctca
     5881 acttgaaaaa tggtttacaa aagtttcacc aaaaccaatt cgtattttct ttgtacctat
     5941 gatgagctta ttgattacag ttcctattgc tttgttgctt ctaggaccat taggttataa
     6001 tgtaggaaca gctttctctt ctatcattgt gtctttgtac aatcaatttg gatgggttgc
     6061 aaccggagtc ttagcagctg tactgccatt aatggttgta acgggtatgc ataaagcaat
     6121 gatcccttac gctgtttcat ccatgagtga attgggtgca gaacttcttt atctcccggc
     6181 ttcattggca cacaatattt cagaagctgg cgcatgcttt gctgtcagta tcaaaacaaa
     6241 agacaaaaaa ttgaaatcta cggctgtttc tgcgggtata tctgcattat tcggaattac
     6301 tgaaccagct ctttacgggg taacgattct gcacaaacgt gttttatatg ccgttatgtt
     6361 ttctagcctg atcgggggag catttgcagg aatcgttgca cttcaagcct ttgctttagt
     6421 gggtcctggg attgcaagta tcacgatgtt cgctgacaaa gctgacccaa tgaatctggt
     6481 ttgggcattt gcagtatcgg ggatttcatt tgtggttgct tttgctgcag cgttggttct
     6541 ttataaagat aaagaagttg tcaatgaagt tgaaactaag atggaaacgg ccgttgtttc
     6601 agatgctgtt ctttcaagtc ccgtgaatgg aaaagtaatc tcactatcag aagtaaaaga
     6661 cactgttttt tctagtggta tggtaggtga tggagtagca atcattcctg aagaagggat
     6721 attagtagct cctgaagatg gagaaatcac aatggtattt gaaacgaagc atgctattgg
     6781 tttaaaaacg gctaatggtg cagaacttct ttttcatatt ggtattgata ctgttcagtt
     6841 agaaggaaaa gggtttgagg catttgttcg ttctggtgaa aaagtagcca aaggacaaaa
     6901 gctgattgca tttgatattg atttaatcaa agaagcagga ttcgacccca cagttatctg
     6961 tgtggtaacc aataaggata attacactat ccgttcattt aacctagaaa aattggtaac
     7021 caatgatagt gatctcttag ctatcagtcc gatagcttaa acacaagata gtgaaaaaaa
     7081 atatattgaa tgaaggaata gaggagaata gaataatgac aaacacaaca agttttccaa
     7141 aaggattttt atggggcggc gcgatagcag caaatcaagt agaaggtgct tggaatgtag
     7201 acggtaaagg tctttcagta gcggatatgg ctacttataa accaaattta gacaacaaag
     7261 attttgctgc tcatatgcat gtcaccctgc agtctataaa agaagctagt gaagacaaat
     7321 ctgataaatg gtacccaaaa agaaggggcg ttgattttta tcatcactat aaagaagatt
     7381 tagctttatt tgccgaaatg ggctttaaaa ccttgcgcct ctctattgct tggactcgct
     7441 tgttccctac aggagaagag ctggaaccga atgaaaaagg ggttgcattt tatgaaaacg
     7501 tctttaaaga aatgcaacgt ttaggaattg agccgattgt gacactatct cattatgaaa
     7561 tgccattagc tttagcagtt aaatataatg gctgggtgga gcgtcgagta attgatgatt
     7621 ttgttcgctt cgctaacgtt tgttttgatc gcttcggcaa atacgtaaaa tattggctga
     7681 catttaatga gatagacagc atccaccgtc atccatttac aacagctgga attatccaag
     7741 agaaaagcgc agaaggtcaa gaagctcaag atatctatca agcattgcat catcaatttg
     7801 ttgcgagtgc gttagtgaca aaagatgctc atgaaaaaat tccaggaagc caagttggtt
     7861 gtatgttaac gaaattgatg acttatccat taacttgcgc accagaagac gttgagctga
     7921 ccttgaaaaa gaacttagaa aataattttt atgcagatgt tcaagttaag ggagaatatc
     7981 ctgctctaat cgaaaaggat ttggaaaatc ggggaattca gattaaaatg gaagatgaag
     8041 atcgagaaat tttaaagaaa cataccgtag attttgtttc tttcagctat tacatgtcca
     8101 tggcagaatc agctgatcca aacgcagaac gcacaccggg aaatactgtt ttaggggtta
     8161 agaatcctca tttgccttca acagattggg gctggcaaat cgatccaaaa ggtttgaaaa
     8221 tttcgttgat cgaactttat gatcgttacc aagtaccgtt gatgattgtg gaaaatggta
     8281 tgggagcagt cgatcaggta gtagatggta aaatccatga tccgtaccgc atcgactact
     8341 ttaaagcaca cttccaacaa atgaaagaag caatcgatga aggtgtagat ttgatagctt
     8401 atactagctg gggaccaatt gatttagtta gtgcaggaac ttctcaaatg tctaaacgat
     8461 atggatttat ttatgtggat gcagacgatg aaggaaatgg gacatataac cgcttgaaaa
     8521 aggattcgtt caactggtac aaagaagtga tcgaaacaaa cggtgcatcc ttattcgagt
     8581 agtctcgatc tttttaagca gcgatagggg gataatgagt gatttcaata aaaaaagtat
     8641 tgaattcaag tgtggtactt gttgaagaaa atggcaaaga aatgattgca ttaggtaaag
     8701 ggataggctt tggacagaaa gctggggatg aaatatcagg agataaagta gataaaatat
     8761 ttctgccttt tgaagaaaag aaatcttcac aactattaga gttagttgaa gaaataccta
     8821 ttgaattttt tgaaatcact cgtactatcg tttttgaagc agaaaaagga ttagataaaa
     8881 aactgaatcc tggagtctat ttaaccttaa cagaccacct gcattttgca gttgaacgcg
     8941 cacaaaaagg ggtaaatatt gcaaaccgtt tgtactggga aataaaaatc tactatccca
     9001 aagaatttca aattgggaag tacgcactca cccttttgaa cgagcaatat ggaattgatc
     9061 ttcctaaaga ggaagcctca aacattgctt tccatttgat caatgcacaa tcggaagatc
     9121 ctgatagcgg cgctggattc aagtatgcaa aaatgatcgg cgggatcgtc aatttggtcc
     9181 ggtattcgtt gcagaaagat atcgatacta attcgatcca ttatgcccgc ttcattacac
     9241 atgtacgttt ctttgtagaa agatattatt ctgatgcttt gttggatgat ggtgaggaag
     9301 aattctacaa tcaaatgtgg atcttatacc cgtctgccat ggaaagcgca gctaagatca
     9361 aagactacat tgaaaaagtt tatcataaaa caattccgaa cgatgaagtt gtatacctag
     9421 gtgtccacat caatcggttg atgaaacatg ctaatgggtg agcgctcagt aatactattg
     9481 atgcttttaa aatctttaga gatggattat agtaaaaaca aggtgatttc cagctgtttg
     9541 ttatctataa gagacattta aaaaggatta acgtaagata ataaaaaatc
//
LOCUS       DAZKOR010000097         9416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000097 DAZKOR010000000
VERSION     DAZKOR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.099"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG8ZM_002364"
     CDS             168..362
                     /locus_tag="ACG8ZM_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493293.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG8ZM_002365"
     CDS             631..930
                     /locus_tag="ACG8ZM_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493294.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG8ZM_002366"
     CDS             complement(1027..1461)
                     /locus_tag="ACG8ZM_002366"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0493295.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG8ZM_002367"
     CDS             complement(1693..2403)
                     /locus_tag="ACG8ZM_002367"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0493296.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG8ZM_002368"
     CDS             complement(2393..3253)
                     /locus_tag="ACG8ZM_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0493297.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG8ZM_002369"
     CDS             complement(3258..3896)
                     /locus_tag="ACG8ZM_002369"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0493298.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG8ZM_002370"
     CDS             complement(3911..4210)
                     /locus_tag="ACG8ZM_002370"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0493299.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG8ZM_002371"
     CDS             complement(4253..5737)
                     /locus_tag="ACG8ZM_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0493300.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG8ZM_002372"
     CDS             complement(5757..6218)
                     /locus_tag="ACG8ZM_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0493301.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8ZM_002373"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8ZM_002373"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0493302.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG8ZM_002374"
     CDS             7521..8291
                     /locus_tag="ACG8ZM_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0493303.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG8ZM_002375"
     CDS             complement(8564..9391)
                     /locus_tag="ACG8ZM_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493304.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2925 a         1927 c         1481 g         3083 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaat
//
LOCUS       DAZKOR010000098         9048 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000098 DAZKOR010000000
VERSION     DAZKOR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.100"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..894)
                     /locus_tag="ACG8ZM_002376"
     CDS             complement(<1..894)
                     /locus_tag="ACG8ZM_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFQ0493305.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYD"
     gene            complement(900..2006)
                     /locus_tag="ACG8ZM_002377"
     CDS             complement(900..2006)
                     /locus_tag="ACG8ZM_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0493306.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            complement(2006..3142)
                     /locus_tag="ACG8ZM_002378"
     CDS             complement(2006..3142)
                     /locus_tag="ACG8ZM_002378"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0493307.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(3174..4625)
                     /locus_tag="ACG8ZM_002379"
     CDS             complement(3174..4625)
                     /locus_tag="ACG8ZM_002379"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0493308.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(4669..5433)
                     /locus_tag="ACG8ZM_002380"
     CDS             complement(4669..5433)
                     /locus_tag="ACG8ZM_002380"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0493309.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(5461..6159)
                     /locus_tag="ACG8ZM_002381"
     CDS             complement(5461..6159)
                     /locus_tag="ACG8ZM_002381"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0493310.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(6171..6950)
                     /locus_tag="ACG8ZM_002382"
     CDS             complement(6171..6950)
                     /locus_tag="ACG8ZM_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0493311.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6966..7910)
                     /locus_tag="ACG8ZM_002383"
     CDS             complement(6966..7910)
                     /locus_tag="ACG8ZM_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0493312.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7956..8735)
                     /locus_tag="ACG8ZM_002384"
     CDS             complement(7956..8735)
                     /locus_tag="ACG8ZM_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493313.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2597 a         1865 c         1330 g         3256 t
ORIGIN      
        1 atcatatata ttatttaaat ttttaatcaa ttcgtcacat aatggattgt tactaggtat
       61 tttatcaaat gtctgtgcta tttcatgatt tattctctgt gctaaaacaa tcatataatc
      121 tatcatcaaa taatccacca tcatatcatt atttttccaa taatttaaaa aaaagtctct
      181 aattatagca aacatccatc tagactcttg attacatgct aatgaataac ccgcaaaata
      241 tcctgatgct acggatgcgt gagcataatc aggcctccta atagaccaaa tatcgatatc
      301 aaaatactca tctggtattg gttttgtaca ataaaatgtg gcgtctaacc acaaacctcc
      361 atgttttgct agcaaagata atcttaataa atcagaataa tttgttctcg taatgattcc
      421 tttgtttttc ttttcttcca cccattttgg tatatcaaca tattgtctat aattatcttc
      481 tgttaaaatt ataacagtat gtttccctgc atttcgtttt attgatttta cacaagcttt
      541 tacaatttcc ggcgattgat ccaatccctg ccaccaacaa acccaaatgc agttagaata
      601 tggtgatttt gattgattaa ttatttgatt ttgactataa tcatatgttt tcaaaaaatc
      661 tgaaaaagtc ttttcataat acttcagcat tacctcatgt ttttttaata aatgttcctt
      721 ctgtttttta ctttcattat atccattacg attcatgact tgaatatcaa ttttagcttg
      781 taacgtggca aatgcttcct tccaagatgt tatctttgca attcgtcttg ttgcattcaa
      841 ttcttcggat atgcgatgta taattctttt tatacttgaa tgtttctgtg tcatattctt
      901 taacctcttt gtattaattc aaataactca taatattttg attctaagat tttgtatgct
      961 gtttgaatat tataatctgc ctttattaaa atcgaacgtt ctgaatctcg atgagagata
     1021 tctacatttg caatctctct cgcccattct tttgccgatt gatttaaact tatgaaattg
     1081 gtcttatcac tgaaagcaac ttctgtagga actttctccg aaaagaaaca cggtaaatct
     1141 gcaaattgag cttcaatacc cactaacgga accccttcaa ataatgatgg caacattaat
     1201 acgtcaaatg cttgataaaa tttttctata tcatttctat ttcctaaaaa tttaatatca
     1261 tcatctatgc cacttttctt tgcataattt tttatttctt cttccatatc accagttcca
     1321 ataattaata atacggaatt attcttaata gcgtgaaatt ctttaaaaat ttcaatcaaa
     1381 aaacgatgat ttttagaata tgaaattctt ccaatatgtc ccattacaaa cgtctgactt
     1441 gaaattccta atttcattcg tacatcattt ctaatacttt catcataata aaatttttta
     1501 gcatccactg catttggaat caaagtgaat ttatcatttc gaaacaaaaa atcacctgct
     1561 acttttccac atgaaaattc atgtgtcaaa acagacgtta acttacatcg ataatattgc
     1621 tttaaaagat atttaaaatc tctatctatc gatgtattat ggctatgtgc tatccgtatc
     1681 ggaatcccag ctttttttgc agcttttaaa ggtaaataac tcatcgcatc aatatgagaa
     1741 tgaacaatct catattctgg atgatcctta aaaaattgtt ccatccattt aaaatattta
     1801 ggataatttt gtggataaag tctcggtgca taatagacct ttcctccaag acttattatt
     1861 tcctcatcat aatcactttt atatggacga tgtgtcaaaa aatcaaactg aattttagtt
     1921 cgatctatat tcctataata gttcattagc atcgtctcca accctgcacg atgcatatca
     1981 ttcacacact gtaatattcg aatcattata tatttcccct atacaaattt aatgtttctt
     2041 gtacgatttt atcccaatta tatttactac aaataaactc agtagcttga ttcttcaaaa
     2101 ctttaaccat ttccgattga ttgcatgcct cttctaatct tatctttaaa tcacttacat
     2161 ccgatttctt aaaaatcata gccttgtctt ctactacttc cgtacactct gagatatttg
     2221 aaactataca acagttccca taactcattg cttctaacaa acttaatggc attccttcta
     2281 aatcacttgg aagtgtatag atatacgcat tactatataa ttcttctaat tcctgtcctt
     2341 gtacaaatcc agtaaatata attcgctcat caccttttgc taattccttt agttcatttg
     2401 caaattcatc tgtatcagag cttcctccgg ctattataag ttttttatct gtctgaacat
     2461 ctttaaatgc ctcaattaga taccgaattc ctttttcggg aaccaatcgc cccaaaaaca
     2521 aaatataaga atcttttgtt aatccatact tttctgtaat caatcctgct gattttattt
     2581 caattttttt aacgccattt ggtataaacc ttgtttttct tccatatata tcttcaaaat
     2641 aattttggac gctttcacta agtacaatta tttcatctgc aaattttaca gcacactttt
     2701 cccctaacat gatgtatgta ctagcaagct tgttccattt ttctctttga tgatctagac
     2761 catgaattgt tgcaacacat cttttcccaa aaagttttgg caaccacaac atagcacatg
     2821 gtccttctgc atgaaaatga acaatatcat attttccgaa tgctgctcta attccaccaa
     2881 atactgaaga actcattgct gcaattcctt ttatatttaa tgtaaaaata gtctttaatt
     2941 ttacaccttt atattcttta tttttatgct ggtcaaattc ctttccgcta acatgatgac
     3001 cacttcgatt atagcaagtc acttcatgcc caagtgcaac cattcgcgtt gccaattctt
     3061 ctaccactat ctcaattcca ccttctcttg atggaattct tttatgacct agcatacaga
     3121 tttttaattt ttttcttgcc atcacgtttt actccttcta tagtatcttt atcttacatg
     3181 gaaccgtttc tatgaagcac agttttgact gtcctaaaca aaattctaaa atctaatccc
     3241 atactccaat tcctgatata ttctgcatca agttttacaa cctcttcgaa gtctgtgata
     3301 tcacttctac cactcacctg ccacattcca gtaatcccag gtttcatcgc aattctcgca
     3361 cgatgatgaa aatcatactt ctcatattct gacttcaagc aaggtctaaa ccccaccagg
     3421 ctcatttttc ccattaggac attataaaat tgtggaaact catccaatga tgtagatcga
     3481 ataaattgtc caattcctgt cttctttgtt ccatcaggaa gtattttatt gccaatgatt
     3541 cttgggtcaa aatccatctt aaacatgaga ttattactca tcttattttc tttcatcaac
     3601 tctgctttgc gttcttctgc atccatatac atactacgga acttatacat cttaaatgtc
     3661 ttaccatttt gaccaatacg ttcttgtgca aagaaaatcg gccctggtga tttgatgtaa
     3721 ataactggtg ctacgaagat aaaaataaca cctgttgcaa tacatccaat aattccagca
     3781 agaatgtcaa tcgttctctt tataaatgct tgcctcatcg tcataacatt aatactagat
     3841 gtaataacag tataatcacc aactcggcca ataaattgtt ttccacgtgt actatcagac
     3901 acttttgtaa gattcatatg tacagcaagt cccatttccg aacatctggc gattaattct
     3961 ttgggatatg gaacactttc gtcaacattg atgaatactt catctaccca attctgtaaa
     4021 agatattccg ctgcattatc agcatttgca actactggga cacctccgat ggtagttcca
     4081 tacatatctc tgtctactac aatcacacca ctaagtttat acatgccata attcttctct
     4141 ttaagattac gaactacatt ttttgcaatc ttttgagatg ttacaataac taacgataca
     4201 tcttcaccgt ttttcatgcg gtgatgcaac aaccctttcc aaagtatacg aattatatat
     4261 gttaataatc catacaccac ccccattata aataaagaaa ctctggaata atcatttcca
     4321 tcctgcattg taataaggta taaactgcac acaagtaaca gcataaaaga ttgctttata
     4381 gaagctacag cctctttata atatcctctt tttaaaacat ttttatatgc ctcaaataaa
     4441 aatataatcg taagattctc aagttctata aaaattgcca tgtggcgata aataggaatc
     4501 acatacggat tccagtcacc ctgtcgcatt acatatgata tataaaaagc aagctggaca
     4561 caaaataagt ctaataaaat aaaatcaata tgtttagacc aaccacttga gttttttcta
     4621 tacatctctc actctctcct catttccttt ttatctacga ataaagaact aaaataaacc
     4681 aaacttcttc ttttttacct cttcatagat tggataattg atctcatccc cgttgatcag
     4741 atcctttgcg acttgtttca tctgtactac tttttcctta ccaaactcct tggcaatctg
     4801 ctcatagcac tcttttaagt aaaaggtgcg cttagagacc ccgtgtgcat cagaagcagc
     4861 catctgcacg agattatgtt tgaccattgt tttggctgtt ttctggatat ctttgccaaa
     4921 tacaccaaca taacttggcg cagtaagttg ggccaaacat cccatatcta ggaacggaag
     4981 caaatgattt gggtcttctc gaaaatgcgc gttgcgttct ggatggacga tcactggtat
     5041 ctttcctagt tctaacaact caaaaaaaag ccgttctgca tataacggca cttccagtgt
     5101 cggaaattcg atcagaagat atgtatcatc taaatctgta aataaaattt catctcggtg
     5161 gatgtcttcg atgagcgttc cggtaatacg tacttcttgt ccttccaata acgtcaaagg
     5221 caactgtctt ttctctaatt cggcttgtaa cgaagcgact agagaaatga cttgtgattt
     5281 ttcattgctg tattttccat tattgtggtg tggtgtgcat aaaatatgcg taatgccttg
     5341 ctgaatcgct ttttcagcca ttgcaatact tgcttctaag ttttctgccc cgtcatcgat
     5401 tcctggtagg atatggcaat gtagatcgat catttctctt gtctcctctc ttcttattcc
     5461 ttaatttccg tagtaataac cagcatcttt gctgtgttct gctccgttgt atacgacacc
     5521 taagactcgt gcttgtacca tatctaacag ttcttttgct ttagtcaatg attcttttcg
     5581 tgacgtattt tcccgtacga ccaataaggt gccatccgtt tttgaagaca tgatctgtgc
     5641 atctgttacg gcaacgactg gcggcatgtc gaagatcact acatcaaata gttgtcttgc
     5701 ttctgctaaa atctgatcca ttcttggcga acttagtagt tctgatggat ttggtggttt
     5761 aggtccactt ggcagaatcg acaaattgtc gactggtgtt cgttggatct catctgctac
     5821 acttcctgat gaactaagaa cagtacttaa tccgcttgcg ttgttcaact tgaacgtttt
     5881 atagatgacg ggcttacgta agtctgcatc gactagcagc acccgttgac cagatttggc
     5941 aaaaacaacg gcaatgtttg ctgcagtggt cgatttccct tctccaggtc ctgatgaagt
     6001 cacaacaatc gttttgattt gctgaccagc tgcggaggca aactggatat tcgtccgaat
     6061 cgtacgatat tgttcagcga ttggtgaaga gggattggtg agtgtgacca aactgactgc
     6121 atgagtttgg gctgcttttt gtttctgtgt tcgtgccatt ttctttcctc ctagactctt
     6181 gagcggcttc tgcgtgatgg cgctgtttta tcgtttgtag cttttggttg tgtctgagct
     6241 ggtccttttt tacggatacc agcattcaat tctttcgctg acatttctgg aactgttcct
     6301 aagattggga atcctaatgt atctgtcaca tatttttcat ctttcactgt ccgatcgagt
     6361 aattccaata agaatgccag acctacacct accatcattc ctaacattaa gccagtcatt
     6421 aaattcaatt tattatttgg cgaaacaggt gtcgtattgg caactgcacc tgaaatgatc
     6481 gagattttat caacattcat cacatctttt gcgttctctt ggaaaacttg tgaagttgtg
     6541 ttagcgatct gttgagcagc cactgcattc gtacttattg cctgaatcga gaacatttgt
     6601 gaattttctg cctgattaac tgagatggca tctttgattt gttcagcagt cattttcaca
     6661 ttgtcttctg tttctaaacg atctttgact tcattgagca ctaaatcact gacgatcata
     6721 tctttatacg tattgatcat ttgcaggttc gtattgacat cattgacatt tgcagtgtct
     6781 gattgtggca gtgtcacgat caattgtgct tgtgaactgt attttggtgt caagatgaaa
     6841 aatgttacga ttcctgagat ggccaagcca gcaaacattg agatgatgat tattaccaag
     6901 tgtttgcgta atgtttcaaa aatttctttc aagctgattg tctcttccat tttttctcca
     6961 ctttcctatt tattctctag attcatttga tcttttaatt cttttttcac acgatcaagt
     7021 tcttgatcac tgactttttg ataggagata ttatcttgca tgaagccttc tccttgcagc
     7081 tgatcttgct taatcgtacc aaatgcctcc cgataatcta aagcaatttt tttcatttca
     7141 tcaaagttca tgtctgtttt catattcgac tctagagcat tcaaaacgga ttgatagttg
     7201 ctgacaccgt ttagactcaa tacttttttc gctacgcctt ctacgatctt tctttggcgc
     7261 ccttgtctac cgtaatcacc atttgggtct tcataacgca tacgagaata agataatgcc
     7321 tgatccccgt tcaatgtaat tttaccgatt ggaaagtcat agccatcttg tgagaaagtg
     7381 aggtcattat tgacttcgat accaccaact gcatcaacga gttcctttag ccctttcata
     7441 ttgatagaga catagtgatc gattgggatg tctacatatt tttgtacggt atccatcgcc
     7501 atggcagctc cgccgaatgc gtaagcgtgg ttgatcttgt cttttgtgtc acgacctacg
     7561 atgtcgacat acgtatcacg aggaatgctg acgatcgttg tctgtttgtc ttcaggactg
     7621 actgtggcaa ccatcatcgt atctgatctt ccttgatcga cccgacctaa atctccagta
     7681 tcaatcccca tcaatagaac agagaaagat tcttgtttgt ccagatcaac tggtttcgtt
     7741 cgttctcctt gggttcgttc cactgattcg taagttttct caatagaatg tgagagatcc
     7801 agatataatt ttgctcctac tcctacaatt gctaatacta atacgataag aactgctaaa
     7861 ataatcagaa atattttttt ccctcttgat tttcttcttc gtcttgccat agtttcctcc
     7921 gtttgctaac cttgcttgaa tcaacgtttt aactgtcaat agcttatatt gctatagccc
     7981 atttgctcag caacagtttg tgcttttgtt gcgtatgctg cgcttatttt ttcattccgg
     8041 atctgctcta tttgactgag caccgtcaaa tattgatcgt aagtagcggt ggcagtcact
     8101 tgaccatcct ttaacatcgt atcgaataac tgatctagtt gcgtgacttg cgcgatctga
     8161 atatcagcga atcctagata ttgcataaca accgttttcc aaggactatc tttaaaaaag
     8221 ctcatatttt cacgtacacg agcgacatct gcacttgtca ccttttcttt gatgatgaca
     8281 tcgtctacag cttgctgcca gtttgataca tttttagtaa ataaatcgtt gacagctgtc
     8341 tggatcttca gttttgattc tgcactatct aactgtgtca cgattgcttt cttctcatct
     8401 tggattgcaa cagcttcttt aggcaattct gattctttga tgtcgtaatc atctgctgaa
     8461 agtttgacag aggatagctt atttgacaaa cttgtgattg tttcttctgt tatttcaggg
     8521 tttagaaaaa ttttttcttt tgtactatac agcttgtcta cttcttttga taaagaagtc
     8581 aaggtgtctt tttgattggt gatggcagat tctgcataat tgatccgttg ctgatgttgc
     8641 cgctgattat tgatatggta tccaaacaaa cagatcgcaa tagcacctgc acctaccgcc
     8701 acagcagtca atgacttgac gaatggcttt tccatagatt cactcctaag tattgatgat
     8761 agataaatta agatataatt cacttacata aaaatgacag ctttttattt aaaaaaacat
     8821 aattaaacta atatattcac agaaatattt gtaaacaagt tttccctata taatcatacc
     8881 acaaactgtt ttcttgacta gggcaattta cgatttaaaa gtaaataaaa cattaataag
     8941 ataaaaatta atcgatttca tttttatttc cataattttc ataggtgttt ttaaaagtta
     9001 cacaaaaaat agaataacaa aaaaattaac attgaattaa taatagtt
//
LOCUS       DAZKOR010000099         8902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000099 DAZKOR010000000
VERSION     DAZKOR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.101"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..837
                     /gene="purR"
                     /locus_tag="ACG8ZM_002385"
     CDS             19..837
                     /gene="purR"
                     /locus_tag="ACG8ZM_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0493314.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1340..2713
                     /gene="glmU"
                     /locus_tag="ACG8ZM_002386"
     CDS             1340..2713
                     /gene="glmU"
                     /locus_tag="ACG8ZM_002386"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0493315.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2807..3778
                     /locus_tag="ACG8ZM_002387"
     CDS             2807..3778
                     /locus_tag="ACG8ZM_002387"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0493316.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3784..3886
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3952..4521
                     /gene="thiT"
                     /locus_tag="ACG8ZM_002388"
     CDS             3952..4521
                     /gene="thiT"
                     /locus_tag="ACG8ZM_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0493317.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4602..5375)
                     /locus_tag="ACG8ZM_002389"
     CDS             complement(4602..5375)
                     /locus_tag="ACG8ZM_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493318.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5642..7342
                     /locus_tag="ACG8ZM_002390"
     CDS             5642..7342
                     /locus_tag="ACG8ZM_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0493319.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7312..7530
                     /locus_tag="ACG8ZM_002391"
     CDS             7312..7530
                     /locus_tag="ACG8ZM_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0493320.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7550..8440
                     /locus_tag="ACG8ZM_002392"
     CDS             7550..8440
                     /locus_tag="ACG8ZM_002392"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0493321.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8458..8742
                     /locus_tag="ACG8ZM_002393"
     CDS             8458..8742
                     /locus_tag="ACG8ZM_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493322.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         2998 a         1527 c         1881 g         2496 t
ORIGIN      
        1 taaaaggagt tggaataggt gaaagtacgt cgcagtgaac gtctagttga catgacacag
       61 tatttattga atcacccgca tgaattgatt tccttaacaa gctttgccga acgttatgaa
      121 tctgctaaat cttctatcag tgaagatttg ggaatcatca agaaaacatt taaagaacga
      181 gggtatggaa ccttggaaac aatcccgggt gctgcaggtg gtgtattgtt catccctgaa
      241 atttcatatg aagaggcgaa aaaatatatt gaatctctag cagaacgtct atcagaacaa
      301 gaccgtctgc ttccaggagg atatgtttat ctctctgatt tgttaggaga acctgaactg
      361 ctgcgtcaag ttggaagaat cattgcttct aaataccttg gtgaaaaaat cgatgctgta
      421 atgactgtag ccacaaaagg cgtacctatt gcccaagctg tgtcctatta tctgaatgcg
      481 ccattcgtta ttgtacgccg ggactcaaaa ataacagaag gatcaacagt aagtgtcaac
      541 tatgtgtctg gatcttctga aagaattgaa aagatggaac tgtctaagag aagtttgaaa
      601 cgtggttcac gtgtccttgt agtggatgat ttcatgaaag gcggcggaac agtcaacgga
      661 atgaagagct tgattgaaga attcgaatca gaacttgttg gcatcactgt atttgccgaa
      721 tcaaaattca acggtcatcg cgccatcgat gattatacgt cactgcttta tgtcaaagat
      781 gtagatactc aaacgaaaaa tattacggtt gtgccaggaa attatttttc agaataagag
      841 gttgtgaaaa agctgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcca
      901 cattttttca taaatttggc ttaataccga gaaactggct tttgaacacc gtttatctag
      961 aggttgtgaa atcagcgtcc tgtcctgagt attagctcaa taaacggaaa aattgctcct
     1021 catattttgc cgtttattga gctaataccg aaggtcagct gattgaaaaa cgtttaatga
     1081 agaggttgta agcctaccgt ttaactctaa gtcacaagaa acgatttcaa aaaacagctt
     1141 ttcatgtttt atgagtagtg tgacttgtga ccgaggagtt ggcaggagag caccggttac
     1201 caaatagata ggcccgttat ggtctatcta tttttttatc cagaaaacgc tgggataaaa
     1261 gaaaatcact tgttttgttg aaaaaatggg tatactggga taaactgaaa tcagcaaact
     1321 atatttagga gtgacgcagt tggacgcacg ttatgcaatt attttggcgg cagggaaagg
     1381 tacccgcatg aagtcaaaat tatataaagt tttacatccc gtttctggta aacctatggt
     1441 agaacatatc attaatcggg tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg
     1501 acacggagca gaacaagtta aagcacaact aggagaacgc agtaaatatg cgttacaagc
     1561 agaacaattg ggaacaggac atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa
     1621 aggaacaacg ttagttatca gtggcgatac accattattg actactgaaa cgctgaacaa
     1681 tctttttgaa tatcaccaag gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga
     1741 aaacccaaca ggatatggac gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt
     1801 ggaacaaaaa gatgcaacac cagaagaagc tcttgtacaa gaaatcaata caggaactta
     1861 ctgttttgac aacgaagctt tgttcgatgc gttaagcaaa gttggaacaa acaatgctca
     1921 aggtgaatac tatctaacag atatcatcga aatcttaaaa gaagaaggcc atacagttgc
     1981 tgcttatcaa acggatgatt ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc
     2041 taaagcaaat gagatcatga gaaagcgaat taaccagatg catatggtca atggcgtaag
     2101 ctttgtcgat tcagcaacga catacatcga tgcaggcgta gaaatcggtc cagacacatt
     2161 gatcgaagca ggtgtacaaa tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg
     2221 gtcacattca aaaatcgtag acagtaggat tgaggatcac gtcgtcattg aaaactcagt
     2281 cattgaaagc agccatgtca agaaacatgc agatgtggga ccttatgcac atttacgacc
     2341 aaaagctgaa attggcgaaa acgtccatat cggaaacttt gtggaagtga aaaatgctca
     2401 aatcggcaaa ggaactaaag tcggtcactt gacttatgtt ggtgatgcta cgctgggaga
     2461 agaaatcaat gtcggatgtg gtgttgtctt cgtcaactat gatggaaaga acaaacatca
     2521 tacgactgtc ggcgaccaca gctttattgg ttctagcacg aatatcatcg gacctgtcga
     2581 ggtagctaaa aacagttcga ttgctgccgg gtcaacaatt acagacaata ttccagaata
     2641 tgcgctggca attgcgagag caagacaagt caataaagaa ggatatgcaa aaaaactccc
     2701 ttatctaaac taagactttt gcaaaatcac ttgatttatt taaaaaatat gactactatt
     2761 tattaggtag aattctagag ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta
     2821 ctttgaccca agattaaaaa tttttgcgtt aaattccaat cgcccattag cagagaaaat
     2881 tgcagcagca gtcggcgtcg agttaggaaa atcatctgtt actcaattta gtgatggaga
     2941 aattcaagta aacattgaag aaagtattcg tggatcgcat gtctatgtta ttcaatcaac
     3001 gagcagccca gtcaatgata acttgatgga gttgctgatc atgattgacg cactgaaacg
     3061 agcaagtgca aaaacaatca acgtagtcat gccttattat ggctatgcgc gtcaagaccg
     3121 taaagcacgt gcacgggaac caatcacagc gaaattagta gcaaacatga ttcaaaaagc
     3181 tggtgcaaca agaatgctga ctttggacct tcatgcagtt caaatccaag gtttctttga
     3241 tattccagtc gatcatttga tgggtgcgcc acttattgcc aattatttct tagaaaaagg
     3301 aatcaaaggc gatgatgttg tcgttgtttc accagaccat ggtggtgtca cacgggcacg
     3361 taaattagcg gaatttttga aatctccgat tgcgatcata gacaaacgcc gtccaaaagc
     3421 aaatgttgca gaagtaatga atatcattgg acaagtcaaa ggcaaaactt gtgtactgat
     3481 cgatgatatg atcgatacag caggtacgat cacattggct gccaatgctt tgaaagaagc
     3541 tggtgcaact agtgtgtatg cttcatgtac acatccagta ttatccggcc ctgctttaca
     3601 aagaatagaa gattcagcta tcgagcgtct agttgttaca gactcgatct atcttccaga
     3661 agatcgtaaa atcgacaaaa tcgatgaagt aagtgttggc ggactaatgg gagatgcaat
     3721 caaacgtatc catgaaaaca aaccagttag tccattgttt gaaacaaaaa ataaataata
     3781 aataaactca caaaggggag tccaattggg ctgagattga atctatttct aaacccttcg
     3841 tacctgtatc ggttatgcga gcgtaggaat tgtgaataaa cagctgtttg actattttcg
     3901 aaagctgctt cctcctttgt gactttttta ttcaaaggag gaagtttttt tatgggaaaa
     3961 aatagagttt gggtagaagg aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt
     4021 ccgatccaga tcggcagcag tttttctatt tcattagggc agattccatt gactcttttt
     4081 gcgttaagaa gagggacaaa ggcagggatg ctggcaggtt tcatttgggg catcctacat
     4141 tttccgttgg gacaagttta ttatttaagt gtcgttcaag tgttgattga gtacccgatt
     4201 gcctatactt ttgcaggcgc agcagggctc atggcttcaa atgtccaaaa aggctatgta
     4261 gacgataata cgaaacaaat tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt
     4321 tccagatatt tttggcattt tattgcagga gtggttttct ggggagcgta tgcactatgg
     4381 gggatgaatc cttggctctt ttctttcgta atgaatggtg ccagcgggtt gagtacagct
     4441 attgtaacga tcatcgtttt attagggatc caacaaacga ctccttcgat ttttgtgcca
     4501 gataaacgac aggagctgta aataaaaaag aagcaagaaa cttgagacag aatcatgtcc
     4561 tagtttcttg tttccttttt attttttgat aagaaataga tctacttcaa tggcagataa
     4621 gaaagatcgg catcttctac caattgccag tgtcgatcgg tgacagagta gattaaagta
     4681 gcgatgattt cctcttctcg ataccgggtg ccttttaatt tccaaatata atcttcaatc
     4741 acatagattg ggatacgata tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg
     4801 atcccttcct gcatcaaatt acgcatttct ttcaataatt ccacactgat tttttcaaca
     4861 ggtgtttctt ttttgaaaaa agattttttt acattgatct tatctgacaa gatcgaactt
     4921 aatttactag caaaaatcct ataaaattca tctaaatagc gataataaat ccgattcagc
     4981 tgatcattaa gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga
     5041 agatgtgttt tcatatgacc aaaatataat aattctgaaa tttctaaagg tgtcaattct
     5101 ttcagcatcg ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc acgcctgtta
     5161 cgagaagagg aaaagaaacg ctgaatattt tctgtaccac ggattacttt cagccctgta
     5221 tgggattcat attctccagc agaagcagat ggatcaagca agagcaagtt ttgaggaaaa
     5281 tgcacgatac tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac agcattactt
     5341 gtggtatcaa tatgtacata aactgactca gtcatcatgt tcctcctctt atttcctctt
     5401 aatctctttt attttacaac atttgcatta aaaaagcttg tttcttttat tttaaaattc
     5461 cattacaaaa tcacgttttc attgaaaaca ctctcatttt caattaattt ttgtgatttt
     5521 tgtatttttt tatttaaagg aggttttatg aattgttttc ttatggaatc tacaaagtcg
     5581 aatacgagac tcatgacctt tttatgacgt actggtatac ttgaagaaaa aggagagcgc
     5641 gatggagata aaaaaagttt acttttcaca gaaaatgtcg ctgtattcgc ctatccagca
     5701 agcaaaacaa ccccttccat tttttcaaag tcatcaagaa agtagagacg ctttttggcg
     5761 ttctgcaaaa aagcaatgtg cttttaaagc agtatcgaaa aaatataccg tctattttac
     5821 tattgctaaa agtagaggag atgttatttt taataatctg ttgattgaag gtgaaacttt
     5881 tgaatggaaa aaattttttc ggtatatgga agcttgcgct cgttttttta ttaaagaaaa
     5941 ctgttgtttt ttatttcaat ctcctctatc agaagaatgg ttgcgtattt ttcataaaaa
     6001 cggctatcaa ggaaccacac aattaaataa aaagctggtc tatcatacag cacttgtttt
     6061 aggtggagga ggagcgcacg gtgcttatca gattggtgtg tggcaggcgc tgaaggaaca
     6121 tgatatcaac tttgagatta tcacaggtac ctcggtaggt gcgttgaacg gcgcattgat
     6181 tttacaagga gatatgaaaa aagctgtgaa tttatggaaa aagctgtcga caagacaagt
     6241 gcttgcgtta ccagaaatgg ctgcagttga agaccttcgt gaacgcttct atcgagaaag
     6301 acgccaaatg acgaaaacag cactaattga aggcggggtt tcttctgctc cattggaaaa
     6361 gatggtcaaa gacaatttgg atttgtcctt actcaaacat aatcccaaaa tacgtttata
     6421 tacggtgtct actaaactgc cagaactagc ggaagtagtg actgatatcc aacagacaaa
     6481 aacagaagaa ataccagatt ggatactcgc ctctgcttca ttttatcccg ctatggctta
     6541 tcgaaagatc ggaaaaaata aatatgcaga cggtgggtat cgcaacaaaa tacctattga
     6601 tattgcgatt aacaaaggag caacagaggc atttgtcgta gatgtgcaag ggccaggccc
     6661 agcgaaaaaa attcgtatgc cagacatttt tatccattgg aaatgtcaaa cattgtggac
     6721 actaggcagc tttctgcttt ttgatagcca aaggaaccaa ttaaatttac aattaggcta
     6781 tctggaaatg aaaaaacgat taggctatta ctatgggaac tggtatacat ttcattctgt
     6841 taaacctgca agaacatact ggcgaggatt tttgtcttac ttaacaaaag aaattcagtt
     6901 agaattatct tttttcaaaa gcataaaatt ttggcaaaaa ctgcgtaatt tatataagaa
     6961 cagggtcgta ccagagacat gcggattggc gatgctagaa cttttagcaa aaaaacattt
     7021 tttactgccc aatgaaatct atcaagtaga catgatgatt caaatgatct gccaacaaga
     7081 tataaaaagt atgccggatg atttattggc acaaattggt caattaagta ctgaagaatg
     7141 gcgaaggtat cgaaaatacc agcaaaaaat acaaaatgag cgcacgaaaa ctgtttattt
     7201 tgattatctg ctacaaggaa aacgcaagga tcgtttgcag catgaattgc ttaagcaacc
     7261 agttgacaca ttactgatcc tatatttata ttatctcaag gaggaacaac catggcacaa
     7321 gaattttcat atgaaatcgt agaagaaatc gctatattgt cagaaaacaa caaggggtgg
     7381 cgtaaagaac tgaacctagt cagctggaat ggtcgcccgc caaaattcga tcttcgagat
     7441 tgggcgccag accatgaaaa aatggggaaa ggcctgacat tgacgaacga agagtttgaa
     7501 caactgcaaa aagcaataga aaatatgtaa tgaggaagga ccctgtcgaa tgaaaagtat
     7561 gactggtttt ggcaaagcca tccgcgaaac aaaagcgtat cagttagata ttgaaataaa
     7621 aagtgtcaac cagcgttttc tagatgttca aatacgaagt cctaaaatcc tgaactttgt
     7681 tgaaaacgat tttcgtcagc ttattaaaca gcagttaagc cgcggtcgaa tcgaagtatt
     7741 tgtgaatcta gcttatattg gcaaaaacga aaaacagatc gtcatcaact gggaattgat
     7801 tgattcactt atcactcaat tgacagacgg aattcaacaa cgatacggta gtcaaaatca
     7861 agtgtcaatt agtcggatgg cggaacgctt cgcgctggac gaaaacttcg taacggtaga
     7921 agaaaaaccg gaagagagca tggaagaatt ggcacagtta gctctagata cattgaaaag
     7981 cgcacttgat tctattgagg cgagtcgaga aaaagaaggt caagctttag ctttagtgtt
     8041 gaaaaagaat actgaagaat tcaaagaagt actaaaaaaa ctcgatagtt ttgtagaaat
     8101 ctatgaaaag gaatttcaaa taaggtatca aaaaaagctg gaggaatttc ttggagcgaa
     8161 agtcgatgag caacggttgt tgacggagct agccatcttg ttggaacgcg gagatattca
     8221 cgaggaactg gatcgtctgg cgatccatgt acaaaaacta gatgaactta tcgaagcaca
     8281 atgtcctgta ggtcgggaat tagatttcct gatccaagaa atgaatcggg aaatcaacac
     8341 gatcggttcc aaatcaaatg caatcgaaat taaaaatcaa gtcgtccagt ctaagacgat
     8401 cttagaaaaa atcagagaac aagtacaaaa tattgaatga cgaaagaggt agagataatg
     8461 aaatattatg acgtaacgtt ccacgaatta agtggaaaaa ctgtggttaa acgggatatc
     8521 ccttctgaaa aaaacggctt tgacgtatgg aaagatgcct gtgcagacta taatgagaac
     8581 gaactgttta ttttgatcaa cgacggtgct tacgtgacga tgaatcggaa atttattgtt
     8641 cgaattgaca cagaagaggt agaagatccg acagaaaaag cacgttctcg aaaagatgag
     8701 atcatgggtg tagtgaatac tttgtccaat atgggctttt agcaaggatg ctgaacaaag
     8761 aaatacaata aataatcaga attggatttc ttcgcttagc ttctttgctc taggaataca
     8821 tttaaaggtt gtgacaaaat tcgtgtcaca atcttttttt attttgaata agtgatgttc
     8881 aaaaaccatt ttctcggtat ta
//
LOCUS       DAZKOR010000100         8471 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000100 DAZKOR010000000
VERSION     DAZKOR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.102"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..242)
                     /locus_tag="ACG8ZM_002394"
     CDS             complement(<1..242)
                     /locus_tag="ACG8ZM_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493323.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTA"
     gene            375..749
                     /locus_tag="ACG8ZM_002395"
     CDS             375..749
                     /locus_tag="ACG8ZM_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HFQ0493324.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            803..2689
                     /locus_tag="ACG8ZM_002396"
     CDS             803..2689
                     /locus_tag="ACG8ZM_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493325.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQ
                     YKDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILA
                     RNDSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLK
                     KYGTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNS
                     FDTIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIV
                     TLSIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLK
                     YWERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQE
                     NNKEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTT
                     YILVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYF
                     SFNTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQ
                     INPYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQVKSRLLCKMLYNVFTIS
                     TYQN"
     gene            complement(2673..3845)
                     /locus_tag="ACG8ZM_002397"
     CDS             complement(2673..3845)
                     /locus_tag="ACG8ZM_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFQ0493326.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            4258..4878
                     /locus_tag="ACG8ZM_002398"
     CDS             4258..4878
                     /locus_tag="ACG8ZM_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0493327.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            4992..5129
                     /locus_tag="ACG8ZM_002399"
     CDS             4992..5129
                     /locus_tag="ACG8ZM_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493328.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESY
                     C"
     gene            5571..5951
                     /gene="mobC"
                     /locus_tag="ACG8ZM_002400"
     CDS             5571..5951
                     /gene="mobC"
                     /locus_tag="ACG8ZM_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFQ0493329.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            5933..6847
                     /locus_tag="ACG8ZM_002401"
     CDS             5933..6847
                     /locus_tag="ACG8ZM_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0493330.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            6807..7085
                     /locus_tag="ACG8ZM_002402"
     CDS             6807..7085
                     /locus_tag="ACG8ZM_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493331.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDL
                     QVKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
     gene            7087..7776
                     /locus_tag="ACG8ZM_002403"
     CDS             7087..7776
                     /locus_tag="ACG8ZM_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493332.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(7897..7983)
                     /locus_tag="ACG8ZM_002404"
     CDS             complement(7897..7983)
                     /locus_tag="ACG8ZM_002404"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0493333.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
BASE COUNT         3046 a         1357 c         1355 g         2713 t
ORIGIN      
        1 atagctgtca ataccagtaa attatttttt ttattttttg aattatcttc atttaaaata
       61 gaatttatgt tagtactata aaattcacaa atttttttta gtgtctctat atctggtgtt
      121 gttttgttat tttcccactt tgaaatagtt tgacgtgtaa cgttaaattt tcttgccaca
      181 tattcttgag tatacccttt tttcactctt aattctctta gcttagatcc taaatcctcc
      241 attttttctc ctatgtaaac gattatttac ataaaatttt atcataaaaa tactaacgcg
      301 ccaaaaaagt tctttatttt ataaaacaca ctaaaataaa ataattctag taaaaaagat
      361 aggagaaata agttatgaaa aaatttttat gtctttctgc tttaatgggt gtcttattaa
      421 ctagtggagg aatagctagt gccacagaag ctttaaatct agatgtaccc gaagaccata
      481 ccgccattta cggaggagga atggaagcta cagaagaagg attttcttct agtaaattga
      541 gatatgctgc tggcggcggg gatttcaact gtggggttaa tggatttaaa gtttatgcaa
      601 attactacca tgctagagct aaacatagtg caacagctaa aaatggacga ggcggtcaag
      661 tacgatcagt tcagaaagct ggagtaagag cttatgcaac atgcaatgcc acacttactg
      721 gaaatactgg ttggtggaat gtttattaaa aaagagagtt taaactctct tttttattta
      781 catttgaaag gaaattcaaa atatgttaaa aaaaataaac gctgttttat cactttttgt
      841 ctgcattaca atactatttt tttctactaa tgttataaat ggtataaaga cttctgaagc
      901 gctatataaa aataccgctg aaattcatat gcaatataaa gatgatatgg actttaacag
      961 ttatataaac gctttgtatg aaatatctac acaagaaaat attaatattt ctcaatatag
     1021 ttttactgct gttaaccaac taactattct tgctacaaac cctaacgcta acaaacactg
     1081 gaaattttct aaaaaaaata tattagcaag aaacgattct aaagtccatt atactaataa
     1141 caaaaattct aagtaccaat taaaacttcc aaataatttt ttaaatataa atatttatcc
     1201 tttttctcat gtggaaaata ttggattaag tgaaatatta tatgtacaag gaaactcaaa
     1261 caaattaatt cctgtactga aaaaatatgg aacacctact atttccaata tcaatcaatc
     1321 agatactttt caaattaata ctaatattgt tcttgttatc tgctatctta gtatatttat
     1381 tgtaattact acaattatat ttgctttttc aaaaatgaaa gaaatcactc taaaaaaaat
     1441 gttaggctat aactcttttg atactataaa agattctatt aaagagttaa acctcttatt
     1501 ttgttctttg attataactt tcattttatc tatttttttg agcatatatt acagtaacga
     1561 aaattttttc ctatcatttc ttctatgtag tatttttcat tttataatta gttttttatt
     1621 tatcatagtt actctaagta tctatcattt atctagtata aaaaattatc taaaaaattc
     1681 acgaccactt aagttaaatc tttgtatatt aaatatctgt ctattcttaa gcatgatcct
     1741 tctgttaata gcttctacaa aagtaataaa tacccataat gaagccgaaa ataattctct
     1801 taaatattgg gaaagaacta ctaatttata caaaacaaat attactaatc aattaaatag
     1861 aaacaacacc gtagaagaaa ataactactt gaaaaaagct agtaaatttg tttataaaat
     1921 acagaagaat tataaaactt ttattatcgc gccatacaat tacgcaacta tccaagagaa
     1981 taataaagaa ttttttattg gacaaaaagt ttatccaaat tttgaagact atgttagtca
     2041 acctgctggt gcaggaatat gtgttgattt aaactatcta aaatattaca atcctatttc
     2101 ttttgaagaa tctactgact taaatttaat aaactctgat ccactaacta cttacattct
     2161 agtacctaaa aaatataaag aatatgcaaa aatgattgaa aaaaattatt tagaagacat
     2221 acagtttaga cttgaggact ctcctcctca agctccttat aaaaatccta atttgaaaaa
     2281 tttaaaaata aaactaatat ttgttaataa taaccaaaag tattttagtt ttaatacctt
     2341 atatggaaag gcaaaagata actatacaat aactgatccc atagttagag ttattaatcc
     2401 agaaataaca gaatcattat tttggggaaa tatattgact agtgatggtg gattattttt
     2461 tgatcagaaa catacttcaa accaaaactt ttttaatcaa attaatccgt atataaaaga
     2521 atttaaccta gaaaatatca taaatttttc ttattctgtt tttcaagaaa ctggtgaatt
     2581 attatctcaa aaaaaagtaa ataaattaaa tgcatatcta caagtcaagt ccagactcct
     2641 gtgtaaaatg ctatacaatg tttttaccat ttctacttat caaaattgat gtattttctt
     2701 gaagaataaa tccattcatc atgtaggtcc ataagaacgg ctccaattaa gcgattggct
     2761 gatgtttgat tggggaagat gcgaataatc ttttctcttc tgcgtacttc ttgattcagt
     2821 cgttcaatta gattggtact ctttagtcga ttgtgggaat ttccttgtac ggtatattga
     2881 aaggcgtctt cgaatccatc atccaatgat gcgcaagctt ttgaatattt tggttgatcg
     2941 atataatcat gaatcaatcg atttttagcc tcacgcgcta agttaatatc tgtgaactta
     3001 aaaattcctt taacagcttc tctgaaagat tttgaatttt ttttaggaat ggtggtaaag
     3061 atatttctta ggaagtgaac ttggcatctt tgccaactta cgttggtgaa ggattttcta
     3121 atggcagaga ctaatccttt gtgcgcatca gaaataacga gttccgtacc ttgtaaaccg
     3181 cgttctttta ggtattcaaa aaatgttgtc caggtctctt cgctttcgcc actttgaatc
     3241 atgaagccga taatttcacg gtcgccatct ttggttattc caatcgctat atgacagctt
     3301 tttgagagta ctcgattttc ttctcgtact tttatataga gtacatcggt cattaagtaa
     3361 ggataatttt tttctgataa taaacgattc tgccactcgt taaccatagg ttctagctgt
     3421 tctgttaagc tagaaacgaa ggacttagag acggatttac cacaaagttc ttccacaatt
     3481 tttgatactt tacgagttga aacgcctgat acatacattt ccaacattga agccatgagg
     3541 gctttttcgt ttcgttgata acgttcaaac actgtgggtg aaaaatggcc atcacgtgtt
     3601 ctgggtactt ttaattctag cgtgcctaca cgtgtcgtaa agctgcgctc ataatagcca
     3661 tttcgttgac tttgtcggtt ttctgttcgt tcatattctt ttgcttgaat atattctgtt
     3721 cgttgatttt ccattagttg attaaatacc gttgttaaaa tatttttaga aacgtcatcc
     3781 tttacagaat attcaataat gctttgaatc tcttcgcttt tcagtgtaaa atgtacttgg
     3841 gtcatgtaaa agtcctcctg ggtatgtttt tgtcgttaaa aacattgtac cgtaaaagga
     3901 ctgttatatg gcctttttac ttttacacaa ttatacggac tttatctatc tacaatatct
     3961 tttaatatta ggattattta taataattaa ttttcaaatg atttttgtta gttttactat
     4021 aaattcaaaa aaaatagctt taaaaaaaat atttggatat tcaccaataa gtcaagtttt
     4081 agatttatgt ttactccctt ttacaataga gttatttgct actatgtgtt ttcaattgat
     4141 attttcaaaa aacacttact tgtattttat aatttttatg atctttttaa taaatctaat
     4201 tacttctatt attatttata aaaaaaatag ttttaaatat tttaaggaga atattttatg
     4261 atattatcag ttgagaattt caccctgtca attaataaaa aaaagatcaa acaaaattgg
     4321 aacttttcaa tatcagaaaa agattttatt atagtaacag gaaaaagtgg tagtggaaaa
     4381 actactctgt taaataattt aagcctaata gataaacaat ataccggtaa cttattctat
     4441 tttgaaaaga aagtttctaa acatgatata caacgtctac gaagaaatca tattagctat
     4501 ttatttcaaa actatgctct tcttgaaaat caaacagtac aatacaattt cgaattagca
     4561 aaaaagttta acagtaatat ttccaatgat catatttata atttattaac agatttttct
     4621 ttgccaaaag atatcttaaa agaaaaaatc tttctcttaa gcggtggaga acaacaaaga
     4681 atagcattaa ttagaaatat gttaaaacca tttgatatat tatttgcaga tgaacctaca
     4741 ggaaatttag ataaagaaaa ctcaactttt ataatgaact ttctgaaaaa tttaacagaa
     4801 aaagaaaaaa aagccgttgt actagtaaca catgacttaa atgttctaaa ctatggaact
     4861 aatattatag aattataatt tttacccgtg atgctagctg atttaaaact agcaccacgg
     4921 gtatttattt tatattccat ctctcctatc ccaaaattta aataatcact taataaaaag
     4981 gaggcaccct agtgatttta ttgaaaaaag agtatgaaaa attttatgat agacttattt
     5041 attctttgca attgccaaaa aaattaaacc gtcaattaca aaagacttat gaaactaaat
     5101 caactgaaga atgggagagt tattgctaga tttttggtag aagtaacaaa aagtggacat
     5161 attatcataa tgactcgccg aaagtctgat aaaaaattgg ctaaaaatat tgctgaatgt
     5221 ttaataaaac tatcagtttt agctaaatag ttagtagttt acacaaaaaa gcatttatgt
     5281 tttttgttgc aaagtaacaa tttcatcttc ggtgaaataa gcgaaacttt acaaaaagtg
     5341 gctagcattt gctagctttt tttgtattgt ggagcgtggc ttttgagggg gattcaaggg
     5401 ggagcttgcg cccctttgac aagcggttat ttgcactaaa tacgtaggta tttggctagc
     5461 aaatatagag cttgccaaac ccttttagtg agctttactc attgtacctt tttaggtaca
     5521 atggtattgc gaacgatacg tccgtaggac gtactgaaag ggtgggactt atgagcgaac
     5581 aaaatcaaaa tttggctagc aattcttcta aaaaatatac ctatcgatcc gagccaaaac
     5641 aaatcagttt tcgagtgagc gaatccgaat ttgcaaagct gaagcaatcg gctgaagctt
     5701 tgcaaatgag tgtgccggct ttcgtgaaag ccaaggcaca aaacgcgcgt ctggtgacac
     5761 cgaaagtggc gccagacatt gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga
     5821 atatcaatca aattgctaag tggtgcaata cacaccaaca tgacgttgct tctggtgacg
     5881 ctcaacgttt atccgaaaac ctaaaaatca tgcaaaagga gctacaaaag atatggcaac
     5941 aattaaggta agccgaacca cttcttgtag tcgtgcaatt aattacgcag aaccacgagc
     6001 aaccattaaa acaggaataa actgtgacgt cgattatgcc aaaagtgaaa tgaaacaaat
     6061 tcgcatgctg tatggaaaag acgaccgtgt acaggcacac ttattgattc aatcttttcg
     6121 accaggagaa attaccgctg aaaaagctaa tcaactaggg gcagagtatg caaaaaaaat
     6181 agcccccgat caccaaacag ctatttacac acatacagac aaagaccata ttcataatca
     6241 cattgtcatt aactccgtta atctggagac tggaaaaaaa tttcaagctc atggtcaagc
     6301 atttctaaac aaatgttatg acctaaatga cgagatttgt cttgcacata acttaccaat
     6361 cactgagcga ggaaagaaac cagaaaaacg aaccatgtct gaaataaaat taaaagaaaa
     6421 aaatgaacct gtttggaaag acgaaattcg gattgcgatc gatcaaacaa tgaaaaacaa
     6481 aaaaaccaga acctatgacc aattttgtaa ttccctcaaa acattgggga ttcattgctt
     6541 caatcgcggg aaaaatttca cttacgaatt gatccacaaa aagaaaaaag tacgatccaa
     6601 cagactagga aaggactacg aaaaggagac gattttacat gagctggaca gacgagaacg
     6661 aacctataac caacagcgaa ccaacgagct acgaagagct cgagctagca ttcgcgaaac
     6721 tgcagaagaa gtacgaagag aacaacgaac tcttacgcct gaacaaccgg aaattagacc
     6781 ggctcgaaga aaccatgaac aacacgttga aagaggaaca gaccaccaac aagaattatc
     6841 gctctaaaac gttaaaagac atagaaaacg aaataaagaa acaacgtgct tttctcgttc
     6901 cttatacgca agaaaggata caaactacga ttgatttaca agttaaaaaa ctccatcaag
     6961 agctattaga ccagcgaaat aaggaacaga aaaaggtgct tattcaacga tcattggatt
     7021 ttcttgcccc cttatgttgc ttagtcctga cactgttcgc attttggtta gtatggggag
     7081 gctaaaatgg cattttattt accctatcta ctcatttttg ttagtatttc aggaagtatt
     7141 tggctcatct ataaaatttt tcaaacaaga tattcattaa aaggctctaa aattcgattt
     7201 aagcgatttt tctttcttgg ctgtatattt tccttgataa tcgtttcaag cggcttattc
     7261 ggcgttttag agggaaataa gcgcgtttct agaagtattt tattaggaaa tgtcactcaa
     7321 aagtatgaat cagcgcgtga taaaaagaaa aaagagcaag cattagcaca aaaaatggaa
     7381 gagtttacca cttgttatga ggaaatgaac gatatttttg tgaatcaaga aaaacgtttg
     7441 acggataaaa acatggaaaa actcactcgt ctttatcaaa acttgccaga aaaacagcaa
     7501 aaagaggttc aagacaacta tgagcgaacc aaaaaagacg tgcaatatgt aaaagataca
     7561 aagatagaag aagcctgtag tgatctattt ggagacacaa acccttggtt tgccagtgaa
     7621 gaagaaaaaa aagaaaaaca acaatctgtc acatatgaac gctatgaaat cttacttcaa
     7681 caagcaacta acatgcaaaa tccgacaaaa aaagaaaccg ccttaaacta tctaaaaagt
     7741 gtcaaggaat ggttagatca gcaacaacaa aattgaattt gatttaaaat catgttaaac
     7801 tgaatgtgca aatgatcaaa cattagatac acgaaagccc cagagaggtc ggattctctg
     7861 gggctttttt tcaggttgat gtcgccttta ttgattttac ttcttgtttc ggttattcaa
     7921 ccaataactg aataaagaga cacaaactcc tacaagaagg gggcaaatga acagttcaaa
     7981 cattagatac acctccttcc agtcgcaaga ctgtcggata ggcgacaagt tattatatct
     8041 taaaaaatta aatttgtgtg gtgtttttaa gtatcacttg ttttagggtt gttagggctt
     8101 gccctgaccg cctgtaaggc gcttctcgac tagattaagg gaaattaatc aagaattgtc
     8161 ttagaacggc ttagacaagc tttcagagtc tcacacctcc tctttgaaca acggttttag
     8221 caaaggagcc ggaggcgact gcgcagtata atatgagagc atagcgatca caccttttta
     8281 gcgaccgtag ggagcaagtt ttccagttta gaaaaattcg cgaacctttt tggaggaaaa
     8341 agtaggagtt ttttaggcgg gaaattcaat gtgctcttaa gatatactgt tagatatact
     8401 gttagatata ctgttaataa taactgttag ggattagcaa ccttatggga gagtaagtat
     8461 tgacataggt a
//
LOCUS       DAZKOR010000101         8314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000101 DAZKOR010000000
VERSION     DAZKOR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.103"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..631)
                     /locus_tag="ACG8ZM_002405"
     CDS             complement(107..631)
                     /locus_tag="ACG8ZM_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0493334.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(624..1097)
                     /gene="tsaE"
                     /locus_tag="ACG8ZM_002406"
     CDS             complement(624..1097)
                     /gene="tsaE"
                     /locus_tag="ACG8ZM_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0493335.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1220..2200)
                     /gene="pta"
                     /locus_tag="ACG8ZM_002407"
     CDS             complement(1220..2200)
                     /gene="pta"
                     /locus_tag="ACG8ZM_002407"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0493336.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2353..3033)
                     /locus_tag="ACG8ZM_002408"
     CDS             complement(2353..3033)
                     /locus_tag="ACG8ZM_002408"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0493337.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3159..4028
                     /locus_tag="ACG8ZM_002409"
     CDS             3159..4028
                     /locus_tag="ACG8ZM_002409"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0493338.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4054..4569
                     /locus_tag="ACG8ZM_002410"
     CDS             4054..4569
                     /locus_tag="ACG8ZM_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0493339.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4616..5179)
                     /locus_tag="ACG8ZM_002411"
     CDS             complement(4616..5179)
                     /locus_tag="ACG8ZM_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0493340.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5234..5413)
                     /locus_tag="ACG8ZM_002412"
     CDS             complement(5234..5413)
                     /locus_tag="ACG8ZM_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493341.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5490..5999)
                     /locus_tag="ACG8ZM_002413"
     CDS             complement(5490..5999)
                     /locus_tag="ACG8ZM_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493342.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6095..6475)
                     /locus_tag="ACG8ZM_002414"
     CDS             complement(6095..6475)
                     /locus_tag="ACG8ZM_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0493343.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6805..7554
                     /gene="pnuC"
                     /locus_tag="ACG8ZM_002415"
     CDS             6805..7554
                     /gene="pnuC"
                     /locus_tag="ACG8ZM_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0493344.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7875..>8314)
                     /locus_tag="ACG8ZM_002416"
     CDS             complement(7875..>8314)
                     /locus_tag="ACG8ZM_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0493345.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT         2374 a         1714 c         1500 g         2726 t
ORIGIN      
        1 tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg tgtgtttttc
       61 tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat tcaatcaaat
      121 aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct ctaggcatga
      181 ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca ttacgaactt
      241 gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg atgatttctt
      301 ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg ataccgactt
      361 ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt ccaataatcg
      421 tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata taatccaact
      481 cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact aaaaattctg
      541 tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct ggcactgctt
      601 cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc tagaattcgc
      661 tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg cttttctaaa
      721 tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc ttcttctaat
      781 agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc taaccctaaa
      841 tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat ccgaccttga
      901 ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat ccctaatcct
      961 tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt caatatcaga
     1021 ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt cattgttaat
     1081 cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc cagtatatcg
     1141 cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct gctgtttttg
     1201 gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata atcgataatt
     1261 tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg ttcaagcctt
     1321 gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta tagccgatat
     1381 ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact tctgaatctg
     1441 gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat tcgccgtcga
     1501 tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact ttgtctactt
     1561 ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt ggttcgatat
     1621 cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct tgcgcagttg
     1681 gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt ccacgtacca
     1741 tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc tgaagtgctg
     1801 ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct gcaatcccca
     1861 tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct gcttgttctt
     1921 tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc cacttatcgt
     1981 aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca tgcgctgcat
     2041 ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct gctttcaacc
     2101 gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt ttgatcccac
     2161 ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct ccagacgttt
     2221 ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta tctgtatcag
     2281 tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact ataacaggaa
     2341 agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt ctcctaacgc
     2401 aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac gacttgcaga
     2461 taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga tcattttgat
     2521 tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc gttcatttaa
     2581 tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag aatacgcttg
     2641 cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag cccatgacac
     2701 tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt tatagatatt
     2761 tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc catgagcttg
     2821 attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt cataaggagt
     2881 caattccaat gcttcataga tatgatacat atctggatgg atcttttggg acgcatattc
     2941 tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg aggacaaaac
     3001 ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat aaaaagaata
     3061 actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga ttaatatggt
     3121 aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta attgcatcag
     3181 atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat gccgaggcga
     3241 tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga aactatcaag
     3301 aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact ttgaacgggg
     3361 cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca aataatcaag
     3421 caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta gcaaccaacg
     3481 atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc atggtggcta
     3541 cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct aagctggagt
     3601 tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag aatctggaag
     3661 ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca gttggaaagc
     3721 agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat atcgaagtga
     3781 accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag cgtgggattt
     3841 ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg ctgcaagtag
     3901 caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat gccaaatata
     3961 caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg attgcagaag
     4021 acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat tcaggaaaag
     4081 cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc cctgtttttg
     4141 atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat gaatggcgat
     4201 ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga gctgtataaa
     4261 gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga cttttattat
     4321 attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata tcaaatccat
     4381 catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg agattactat
     4441 gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc tgcggtttta
     4501 gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta ccgacttaac
     4561 ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca tctttttaga
     4621 tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg acaactttga
     4681 agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca cttagaccga
     4741 tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat cgcgaaccga
     4801 tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat tcgttgggaa
     4861 acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc tctaagatag
     4921 gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa gcaactcctg
     4981 caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga taatttggat
     5041 cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc ttcaaaaatg
     5101 gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga gcatcttctt
     5161 ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg cagaaaaaga
     5221 aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa taaccatgag
     5281 taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt tctttatcat
     5341 tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa ttagggcatt
     5401 tcattttttc catgttatct caacttcctt tcatctttac tctcttagct tactcttata
     5461 ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg acggtcctgt
     5521 tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag aagtgtccac
     5581 tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc tgcttgcggt
     5641 tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt ttccgtgtca
     5701 tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc ttcacaaaaa
     5761 aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac tgcaaaatga
     5821 atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc ttttttcaat
     5881 gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt gagcagtccc
     5941 gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat ttttttcatt
     6001 cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat gaaaaatcga
     6061 atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct aatggcaatc
     6121 cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct ctaatcggtt
     6181 ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg tctactttga
     6241 aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc gcattagcaa
     6301 ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag tcatctcgtt
     6361 ctttgcggta attttctctg attacttcaa atcctaattt attaacataa aaatcacgtg
     6421 atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg ttcatatgag
     6481 cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat tgtaaatgtg
     6541 tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa aaaaacagaa
     6601 agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt aagaaattat
     6661 attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat tgaaagacta
     6721 gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac acactacatt
     6781 tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc tcatcagcta
     6841 aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg ccaggtcatg
     6901 actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg taccacgctt
     6961 ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat tttaggtatt
     7021 ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt aagtatcggt
     7081 gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa agaatggaat
     7141 cataatatgg cttcaaaaat ccgaaaattc actggaaaaa gctggtttat cgcaatcatc
     7201 agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac agatgatcct
     7261 cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat catcagtttc
     7321 ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca aatggtcctt
     7381 tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa cagctctgtt
     7441 tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca aaaagaacgt
     7501 gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc ataaaaaaac
     7561 tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
     7621 ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt
     7681 ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct ctatttccta
     7741 cgattagtca agtaaaaggt gcaagaaaaa tagctcgtaa acgaaagcga caaaatcaaa
     7801 gcacaccaaa taaagccccg ttcgtaaaaa attttcgatc tgggcaaaga agtggtatac
     7861 tgtaagtggt ttagttaatg gtccactgag accgcgtacg catagtcgta ctctgtatgt
     7921 gccctaacca tagcgtgcat acatttcaga agcttgttca tacaagcaac tgtggcaacc
     7981 ttatctttct tagggatagg ttgctttttt aatttgtagt aataatcgac aatatgatta
     8041 ggagctgcgc gttgctggcg aatcatattt cgaatggtga agaaaataat tttccgtgct
     8101 ttgggattac cacgcttatt gatgcggtct tggccagtat acttacctga atgataccgt
     8161 cggatgtcga ttccgacaaa ggcattcaat tgtttatggg ttttaaatcg tgtgatatct
     8221 cctaattcac cgagcaataa agcggctgtg agtgcgccaa tcccagggaa cgtttggtac
     8281 aacaaaaagc aaggagagcc ttcggctttt ttga
//
LOCUS       DAZKOR010000102         7897 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000102 DAZKOR010000000
VERSION     DAZKOR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.104"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..1005
                     /locus_tag="ACG8ZM_002417"
     CDS             142..1005
                     /locus_tag="ACG8ZM_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0493346.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1134..2756)
                     /locus_tag="ACG8ZM_002418"
     CDS             complement(1134..2756)
                     /locus_tag="ACG8ZM_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0493347.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3005..3526
                     /locus_tag="ACG8ZM_002419"
     CDS             3005..3526
                     /locus_tag="ACG8ZM_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0493348.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3712..5442)
                     /gene="spxB"
                     /locus_tag="ACG8ZM_002420"
     CDS             complement(3712..5442)
                     /gene="spxB"
                     /locus_tag="ACG8ZM_002420"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0493349.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5607..6425
                     /locus_tag="ACG8ZM_002421"
     CDS             5607..6425
                     /locus_tag="ACG8ZM_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0493350.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6528..6725)
                     /locus_tag="ACG8ZM_002422"
     CDS             complement(6528..6725)
                     /locus_tag="ACG8ZM_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493351.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6894..7472
                     /locus_tag="ACG8ZM_002423"
     CDS             6894..7472
                     /locus_tag="ACG8ZM_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0493352.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7567..7704
                     /locus_tag="ACG8ZM_002424"
     CDS             7567..7704
                     /locus_tag="ACG8ZM_002424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493353.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2372 a         1405 c         1567 g         2553 t
ORIGIN      
        1 ttcttgaagc tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa
       61 accggttaaa gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt
      121 aacgagaagg ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca
      181 taccatgtat tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat
      241 caagagttag tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg
      301 aaaaatggtg cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc
      361 cgttcttatg cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc
      421 ggctcttaca gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct
      481 gagatctatg tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa
      541 gctgtaagat tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt
      601 aattatttga aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc
      661 ggtaggattt ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt
      721 acggtggata aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg
      781 atccagacaa tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt
      841 tatgtggctt acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag
      901 cagataaaga tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca
      961 ggctatggct gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt
     1021 ctgtaaaaaa gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag
     1081 catcctatct cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc
     1141 cataactcgt tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta
     1201 tcagctcgat cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg
     1261 ataaattgat ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca
     1321 ttcaatgctg tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg
     1381 ttagctttcg aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa
     1441 acattcactg gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt
     1501 aagaaggtgt tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt
     1561 agatcagtat cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca
     1621 ccccaacgta ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt
     1681 gttgttgtta tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa
     1741 gatatatcat ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat
     1801 aagtcgtttc caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa
     1861 ggttggatat cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt
     1921 gattttgatg cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct
     1981 tcttttttgg catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa
     2041 aaatcataat ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta
     2101 catactttgt tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg
     2161 atcaaaaatt cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct
     2221 agcaaaagaa catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt
     2281 ccaccagtta attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc
     2341 agtaaaacag ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct
     2401 aattcagctg ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct
     2461 ttttctttca gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta
     2521 tagtcttcat agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata
     2581 gtaacggaac ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta
     2641 cctgcaccat ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca
     2701 tcatcaaata gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt
     2761 tttcctcact tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg
     2821 taaaagattc aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat
     2881 aaaaataaaa gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat
     2941 ttttgacgtt atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt
     3001 gaaaatggat gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt
     3061 acttgtctca ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt
     3121 ctttgcgatt tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa
     3181 agagctaaca ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt
     3241 tggcgtcttt ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc
     3301 agtatggttt attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt
     3361 aagtgaagga tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat
     3421 tttcttattg tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta
     3481 ttttatgtta tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa
     3541 ataaaaaagc cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat
     3601 catggacaaa tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg
     3661 agaagctata tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct
     3721 cttttcatat aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc
     3781 ttggatttct tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg
     3841 gcgcttgtta ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc
     3901 aaaggcaaat cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc
     3961 tccaacttta ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc
     4021 ttcttgttct gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg
     4081 aagctgatat ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc
     4141 acttaaggta aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac
     4201 accattaccc atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt
     4261 caataaacga atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc
     4321 ttcagcaata cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg
     4381 atcttcaaaa gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta
     4441 ccagctatca gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc
     4501 atcaccgagg atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc
     4561 gatttggata aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc
     4621 gacaaataag atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg
     4681 tccagcaaaa ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga
     4741 agcgacaaca ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg
     4801 cgttccttga ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc
     4861 tagcaaatct ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa
     4921 aggttgttcg tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc
     4981 tttatgctca taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt
     5041 catgactgta cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg
     5101 gaaagagtta tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac
     5161 atggtccatc tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga
     5221 gccgaagaca gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc
     5281 gacttcttca tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc
     5341 cataattgaa ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc
     5401 aagaactttt accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag
     5461 caatgatgat acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa
     5521 ctaattcgtc ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat
     5581 agatagattc aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc
     5641 agcaagcact tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg
     5701 tttttttctt taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg
     5761 gtgtcatgat tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag
     5821 aaaatggaga tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt
     5881 tatatatggc agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag
     5941 gattgcctac ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca
     6001 ttcctgaagg tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga
     6061 cagccgctat attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag
     6121 aaggagcagc tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg
     6181 tttacggaca agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag
     6241 taacgaaagt ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt
     6301 atgtcgtggt ggaagaattg atccctgaag cacagcagac attgtccagc aaacatcatt
     6361 ttgcagtttt tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag
     6421 gataaataaa aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc
     6481 tattttttta gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt
     6541 tttctatgtg tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt
     6601 agaactttga ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg
     6661 aagctagctg caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga
     6721 cgcatacaaa accctcctct tgttcattct ttactataga aaaagtataa cagggattgt
     6781 ttaaaaacac ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat
     6841 tgctttttct ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta
     6901 aaaatcgtct tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt
     6961 tagaacttca tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat
     7021 tttttatcta tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt
     7081 tatttcaagt tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt
     7141 ttgcgctttc gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg
     7201 ctccagaaat gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg
     7261 caaaagaact tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata
     7321 attacaagaa atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt
     7381 tagtagcgcc agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc
     7441 caagtcgtac catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa
     7501 aaatatagaa aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg
     7561 atgcagatgg atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt
     7621 atttatttgg atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca
     7681 ttattcacag caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag
     7741 aaaagcatgt ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt
     7801 aatttttaga aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg
     7861 taagagataa aaataaaaaa ttagtcaaag aagggcg
//
LOCUS       DAZKOR010000103         7647 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000103 DAZKOR010000000
VERSION     DAZKOR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.105"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..2344
                     /locus_tag="ACG8ZM_002425"
     CDS             17..2344
                     /locus_tag="ACG8ZM_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0493354.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2341..3255
                     /locus_tag="ACG8ZM_002426"
                     /pseudo
     CDS             2341..3255
                     /locus_tag="ACG8ZM_002426"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            3328..3843
                     /locus_tag="ACG8ZM_002427"
     CDS             3328..3843
                     /locus_tag="ACG8ZM_002427"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0493355.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            3840..4286
                     /locus_tag="ACG8ZM_002428"
     CDS             3840..4286
                     /locus_tag="ACG8ZM_002428"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0493356.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4436..5251
                     /locus_tag="ACG8ZM_002429"
     CDS             4436..5251
                     /locus_tag="ACG8ZM_002429"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0493357.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5457..6629
                     /locus_tag="ACG8ZM_002430"
     CDS             5457..6629
                     /locus_tag="ACG8ZM_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0493358.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6839..7285)
                     /locus_tag="ACG8ZM_002431"
     CDS             complement(6839..7285)
                     /locus_tag="ACG8ZM_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493359.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(7282..7476)
                     /locus_tag="ACG8ZM_002432"
     CDS             complement(7282..7476)
                     /locus_tag="ACG8ZM_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493360.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2574 a         1444 c         1525 g         2104 t
ORIGIN      
        1 tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc aacgtatatg
       61 tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact tacatcccat
      121 tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta tttatgtaaa
      181 cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa atagtccacc
      241 ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg agacaacctc
      301 aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag cagcaaaagc
      361 agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta ctgttgcggc
      421 cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat atgccaaaga
      481 tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt tacaaggcgt
      541 aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt ttattggctc
      601 cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg tggctaccgc
      661 tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt ttacaccctc
      721 aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg gtatacagtt
      781 agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg ttgatggtaa
      841 gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca atgcgaatcg
      901 gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa cgtacgggac
      961 tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag cagtaacggg
     1021 ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg tcgatacggt
     1081 gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt ttggacagat
     1141 gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag acctgacgac
     1201 taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgatag aaacaaagaa
     1261 ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa tcgtaaagga
     1321 cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg caacagtatc
     1381 aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga tcgttaaata
     1441 tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca gtattgatca
     1501 aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg aagtaactgc
     1561 gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac tagctaaact
     1621 agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct atacgattaa
     1681 gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa aagcaggtgt
     1741 cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc cagtacaagt
     1801 ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata tggttaatgc
     1861 tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag cagcaaatcc
     1921 caagttgtca tttactgata tagccaacac atcaggaact tataccatct ataaagctaa
     1981 cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc actcgatttc
     2041 ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt ataccctaac
     2101 agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag gcgaaggaag
     2161 tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga tcaatccgac
     2221 tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt taggactgat
     2281 ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag gtgaacagcg
     2341 atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt tatcctactg
     2401 gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac atttactttg
     2461 cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga cggaataaat
     2521 atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc tatatatgat
     2581 gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga cgcacaaaga
     2641 aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac aaaaacagta
     2701 gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca aggtcgtgac
     2761 gctgtttacc gatttacgga aataaagact tcagattaag taaaagaaaa atcagcacca
     2821 tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac catccacttg
     2881 tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac agtagagaat
     2941 aagcagacag attttgcaga tggggataaa gtcccttatc tcatcaccgt aacaattcct
     3001 gagaacatca acgagatagg aacctatacg atcaaagata cagctgatcc tcagttatgg
     3061 ctagaaccag aaacgatcga ttttttgatt ggtggagaca agattcatac atttcacaca
     3121 caaaaaataa aacatggttt tgtcttatca gattctggaa aagatcttag ttcgtttgct
     3181 ggaaaaaaat taacgatccg ttaccaaatg acattgaaag aaaacagtga aaaaacgaca
     3241 tttgataacg atgtacgttt gacgactgac aatcaaacag tagaaacaca tcttgccatc
     3301 caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa cgaaacaaaa actagcagat
     3361 gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc aagaaaacgg gataaatcat
     3421 tgggtagcca aagatgattc aagagcaacg atttttactt ccagtaaaga tggaacattt
     3481 tctgtccatg gattgtcttt tggaagttac gaactagtgg agattaaacc gcctaaagga
     3541 tatatattga gtcaatcaac cattctcttt gaagttgaaa aaggcagtta tgatccgctt
     3601 catcctatac cattggaaat catcaacgaa ccaaaagcaa atccgacgaa taatggaaaa
     3661 acgccagttc agatgcagac aaatggtcca tcaaatgaaa aaaatggagg aagctttcca
     3721 aaaacaagcg aagagatgct tggaggtttc tcaattttag ggttattgct agtcctttca
     3781 acagggacag cttggtttta taaaaaacaa caaaaaggaa acaataggag ggaaataaaa
     3841 tgaaaaacat caaaacaaaa atatggacgt ttttaggaac tgctatcatg ctgcttcctt
     3901 tcgttctcgg cctaggaact gcagaggttt ctgcagccgt atcacctaca ccagaaaatg
     3961 ttacggttaa tttgcataaa ttgaaattca cttcagcacc agaaaatcaa ataaacaacg
     4021 gaacggaatt gacatttccg aatagcgaac cgttgaacgg cgttgaattc aacgtatatg
     4081 acatcactgc tacgtactat ccaagtaaag acacagcagt tccagcagac gctacaccct
     4141 tcgcaagtgt aacgacttct ggtgaaggat tagctaacct gactcttcca ggtaaatctg
     4201 atgggaaaga tgctgtatac gtctttgtag aaacaccaaa gcccggtgtc gaaacgtcgc
     4261 caaatatcgt gttaagctta ccctagaccc tgacaaacct ttagacacga ttcacctata
     4321 tgctaaaaat gtcatcaaag aaagcaatat caaaatccag aaagctggga acttttcagg
     4381 ggtagataag ttagcagggg cgcaattcca gatcaaaaat tcagagaatc aattcgtgac
     4441 cggttttgat gaaaacactg gtgtcgctct ctatgatcct gctgagtcag cgttgacatt
     4501 cacaactgat actgaaggat ccatcagtgt caatggatta atagacggca attatacttt
     4561 agttgaaaca aaagcaccag atggttatca attaaccaat ccacaaaaag aaactacttt
     4621 tatagtaaaa aacggaaaat tgcaacaaac aaccggtatc atccatggcg cagatgaagc
     4681 caatcttggg aatccaaacg gtgtagtcaa tcgggaaaat acaactacgg ataacacgat
     4741 tactgtagaa aatgtccaaa actacggtga tttccacttc atcaaacaag atgtgaacac
     4801 gcaagaaaaa ttagccgatg cagaattttc tattctaaga gcaaaagatc caactgttaa
     4861 attaatgaaa aaaatagcaa accttgcctc aggagaatac ccatatgctt ggtcagatga
     4921 agtcacaggt tctgactggg aagaagtgaa attgatttct gattcagaag ggaaattttc
     4981 aatcgaaggg ctgaaacaag gggcatactg ccttcaagaa acaaaagcac cagaaggcta
     5041 cgcattacct aagaatgaag ctgcgtacac tgaatttaca gtagatgatg atccaacaac
     5101 ttctgatgaa acaacgatcg ataaccaacc taaaggaatc ttgccttcaa ctggtggtat
     5161 ggggatcatc gtattcatct tggtaggtac tgctttagtt ggaggagcag tgatctattt
     5221 caaaaaacgc catcaagaaa cagaagctta aactattgga aacaaaaatc atacgataaa
     5281 taaacgatag tgattcaaat ggggagaaag gttgtgacaa gtggtcggtc tcttggtatt
     5341 cgctcaacaa acggcaaaat aggaggaact attttgccgt ttgcttcgct aatactcagg
     5401 tcaaggcgct gatttcacaa cctcttcata tacatattag aaaataggtg gacatgatga
     5461 ataaaagcag aataagaata ctggacatct tcatggcaat cattttagtc gttgggatcg
     5521 ggatcttttc ttatcctttt gtagaagatt cactgaatga ttttcttgcc cagcaaatga
     5581 tcatccatta tcaaaagcaa gcatcgaata agaattcaga ggaaataaaa aaacagcagg
     5641 aaaaaatgac aaagaaaaac caacagttgg cagagcaaaa cgtatcacca gggataggca
     5701 gtttcaacca agcagttgat gcaaaagcat taaaagattt acctagtaat gcctttttta
     5761 tggaacatat gcttggagtc atcgaaatcc caaaaatcaa tgtcagctta cctatattcg
     5821 atcagacaac ggaaatattt ttgcaaaaag gaacgtcatt attggaagaa agttcttatc
     5881 cgactggcgg gaaaaacacc catgccgttc tttctgggca tcgtggactc ccagaagcaa
     5941 aattatttac agatttgcct aagttgaaaa aaggagacca gtttttcatc cagatcaatg
     6001 gaaagacatt ggcgtatcaa gtagaaaaaa tccaagtggt cttacctgat gaagtagatt
     6061 ctttgggtat ccaaaaaggc agagatctcg tgactttgct tacctgtact ccttatatgg
     6121 tcaatacaca tcgactgctt gtgacggggc atcgtattcc ttatcaagca aaagaggcaa
     6181 aaaaagcgat ccaaggaatt gatcaatgga aaaaatggaa attttttatt tggtttatcg
     6241 gtatactact tggatcaatc ggcttagttt ggctactcat cgcttattta gattctttgg
     6301 ccatcgctaa gcgaaactat cctttatctt tttatgtaaa gaacacaaac ggacgaccaa
     6361 tcgaaggaat ggtcttttct gtaaaaacgc tcaacggcaa acactatatt actagagaaa
     6421 aagttccatt tgtgaaagct tctgatgaat acggactagt gagattttct gatttaaaag
     6481 gcaggaacta tcgcttgcaa catgaggagc tcctgcttaa aatccatgta aaacataaac
     6541 attcgaaaca gttttcaatg aaattgaaaa aaggaagata taaactgcga aaagaaaaag
     6601 aggtgtacta tttgattgag aaagaatgaa tgaagatgtc caaaaaagca gagggaacag
     6661 actaaatgaa aaaagagaga agctgacgaa atggacgtgt aaactgttat ttttggttta
     6721 ctatctggaa cgccatcttc tattttttta tgtttcagaa gaattttatt attatagtat
     6781 aacttttgaa ttagtttcca taacatttgg agcaaggggt taaaccacga gcttgagctt
     6841 agcttaaagt ggcaggggta tacgtaccat ttccacattt atgtgtatgg tattttgaac
     6901 cggtcggtgt gacatagaca gtttgttcat tattttgctc aggagctgcg gcttgtgcct
     6961 gctgagaggc ctgttcagct gctgattgct gtcgcgcctg ttctgcttgt tgctgtgcga
     7021 ttgcttcttg cctcgcctgt tctgcttgtt gctgtgcgat tgcttcttgt tgcgcttgtt
     7081 ctgcttgttg tcgtgcaatt tcttcttgct ttttccgttc ttcttctgct tcttttgctt
     7141 tgattgtgga atcaattgca gcaagacggc ttgtcaactc ttgtttgttt tcaggtagcg
     7201 cttggatagc tgctgaagca gttgcatagt tttcttttgt aggatttgct tcagcttgtg
     7261 acaaagcaga ttccgcttct gtcacttttt ttctagtgca gctttttccg ctttttgttt
     7321 ctcttctttt ttcttttcaa tttgttcttg ttctttcgat gaatctgccg tactttcgga
     7381 gatcgtggta ctgctgctgg aagcctgatc tgaacttgtg ctttgagtag tagacgtagt
     7441 agcaccacac ccactcaaca aacttcctgc gaccaaaaac aatgcaacta attttttcgc
     7501 taaatttcct ccttaagtaa aaaacaaccg ttttcaataa atgtcctttt caattgttta
     7561 cttacttgtt ataataattg tgttatgaat agtgattgag gaaacttaat ctaatcccgt
     7621 gttgatacac gggatttttt gctatag
//
LOCUS       DAZKOR010000104         7599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000104 DAZKOR010000000
VERSION     DAZKOR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.106"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..427)
                     /locus_tag="ACG8ZM_002433"
     CDS             complement(137..427)
                     /locus_tag="ACG8ZM_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493361.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="ACG8ZM_002434"
     CDS             complement(545..805)
                     /locus_tag="ACG8ZM_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493362.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="ACG8ZM_002435"
     CDS             complement(942..1355)
                     /locus_tag="ACG8ZM_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0493363.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="ACG8ZM_002436"
     CDS             complement(1811..2296)
                     /locus_tag="ACG8ZM_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493364.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="ACG8ZM_002437"
     CDS             complement(2435..2650)
                     /locus_tag="ACG8ZM_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0493365.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="ACG8ZM_002438"
     CDS             2874..3674
                     /locus_tag="ACG8ZM_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0493366.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="ACG8ZM_002439"
     CDS             3693..5561
                     /locus_tag="ACG8ZM_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0493367.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="ACG8ZM_002440"
     CDS             5554..6045
                     /locus_tag="ACG8ZM_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0493368.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /locus_tag="ACG8ZM_002441"
     CDS             6033..6587
                     /locus_tag="ACG8ZM_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0493369.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..>7599
                     /locus_tag="ACG8ZM_002442"
     CDS             6605..>7599
                     /locus_tag="ACG8ZM_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0493370.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DAZKOR010000105         7579 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000105 DAZKOR010000000
VERSION     DAZKOR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.107"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            228..1535
                     /locus_tag="ACG8ZM_002443"
     CDS             228..1535
                     /locus_tag="ACG8ZM_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0493371.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            1656..2855
                     /locus_tag="ACG8ZM_002444"
     CDS             1656..2855
                     /locus_tag="ACG8ZM_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0493372.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(2882..4150)
                     /locus_tag="ACG8ZM_002445"
     CDS             complement(2882..4150)
                     /locus_tag="ACG8ZM_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0493373.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(4206..4272)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(4320..4961)
                     /locus_tag="ACG8ZM_002446"
     CDS             complement(4320..4961)
                     /locus_tag="ACG8ZM_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFQ0493374.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(5370..5873)
                     /locus_tag="ACG8ZM_002447"
     CDS             complement(5370..5873)
                     /locus_tag="ACG8ZM_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0493375.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(5996..6760)
                     /locus_tag="ACG8ZM_002448"
     CDS             complement(5996..6760)
                     /locus_tag="ACG8ZM_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0493376.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            6868..7143
                     /locus_tag="ACG8ZM_002449"
     CDS             6868..7143
                     /locus_tag="ACG8ZM_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0493377.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         2294 a         1599 c         1368 g         2318 t
ORIGIN      
        1 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
       61 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
      121 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
      181 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
      241 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
      301 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
      361 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
      421 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
      481 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
      541 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
      601 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
      661 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
      721 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
      781 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
      841 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
      901 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
      961 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
     1021 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
     1081 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
     1141 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
     1201 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
     1261 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
     1321 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
     1381 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
     1441 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
     1501 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
     1561 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
     1621 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
     1681 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
     1741 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
     1801 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
     1861 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
     1921 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
     1981 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
     2041 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
     2101 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
     2161 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
     2221 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
     2281 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
     2341 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
     2401 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
     2461 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
     2521 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
     2581 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
     2641 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
     2701 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
     2761 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
     2821 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
     2881 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
     2941 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
     3001 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
     3061 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
     3121 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
     3181 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
     3241 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
     3301 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
     3361 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
     3421 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
     3481 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
     3541 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
     3601 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
     3661 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
     3721 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
     3781 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
     3841 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
     3901 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
     3961 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
     4021 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
     4081 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
     4141 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
     4201 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
     4261 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
     4321 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
     4381 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
     4441 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
     4501 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
     4561 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
     4621 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
     4681 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
     4741 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
     4801 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
     4861 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
     4921 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
     4981 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
     5041 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
     5101 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
     5161 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
     5221 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
     5281 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
     5341 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
     5401 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
     5461 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
     5521 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
     5581 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
     5641 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
     5701 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
     5761 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
     5821 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
     5881 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
     5941 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
     6001 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
     6061 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
     6121 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
     6181 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
     6241 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
     6301 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
     6361 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
     6421 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
     6481 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
     6541 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
     6601 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
     6661 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
     6721 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
     6781 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
     6841 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
     6901 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
     6961 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
     7021 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
     7081 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
     7141 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
     7201 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
     7261 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
     7321 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
     7381 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
     7441 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
     7501 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
     7561 tgattttttg tcttttttt
//
LOCUS       DAZKOR010000106         7480 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000106 DAZKOR010000000
VERSION     DAZKOR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.109"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..635
                     /locus_tag="ACG8ZM_002450"
     CDS             <1..635
                     /locus_tag="ACG8ZM_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0493378.1"
                     /translation="ITHQENRQKNLIVQNLNYGTNQYSVDSSKFELCDTSMKTRDKNN
                     FADSSKNELELYKSYKDSKDIKESHNDDLLESLSTKNNKLDEELIETFIEENLLKQIW
                     GESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVR
                     EQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            711..929
                     /locus_tag="ACG8ZM_002451"
     CDS             711..929
                     /locus_tag="ACG8ZM_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493379.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            916..3594
                     /locus_tag="ACG8ZM_002452"
     CDS             916..3594
                     /locus_tag="ACG8ZM_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0493380.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3585..3782
                     /locus_tag="ACG8ZM_002453"
     CDS             3585..3782
                     /locus_tag="ACG8ZM_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493381.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3786..4397
                     /locus_tag="ACG8ZM_002454"
     CDS             3786..4397
                     /locus_tag="ACG8ZM_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFQ0493382.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4382..5773
                     /locus_tag="ACG8ZM_002455"
     CDS             4382..5773
                     /locus_tag="ACG8ZM_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0493383.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5989..6303
                     /locus_tag="ACG8ZM_002456"
     CDS             5989..6303
                     /locus_tag="ACG8ZM_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493384.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6296..6790
                     /locus_tag="ACG8ZM_002457"
     CDS             6296..6790
                     /locus_tag="ACG8ZM_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493385.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            6858..7172
                     /locus_tag="ACG8ZM_002458"
     CDS             6858..7172
                     /locus_tag="ACG8ZM_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493386.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7271..>7480
                     /locus_tag="ACG8ZM_002459"
     CDS             7271..>7480
                     /locus_tag="ACG8ZM_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0493387.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
BASE COUNT         2879 a         1012 c         1439 g         2150 t
ORIGIN      
        1 agataaccca tcaagaaaat agacaaaaaa acttgatagt tcaaaatttg aactatggaa
       61 caaatcaata ttctgttgat agttcaaaat ttgaactatg cgacacatca atgaaaacaa
      121 gagacaaaaa caattttgct gatagttcaa aaaatgaact agaattatat aagtcttata
      181 aagatagtaa agatattaaa gaatcacata atgatgatct tcttgaatca ctttcaacta
      241 aaaataataa attagatgaa gaattgatcg aaacatttat tgaagaaaat cttcttaaac
      301 aaatatgggg agaaagtatt gtgaattgct taaaactggc tagcaatagt gattatcgtc
      361 aattcgacaa ctggtacaaa aaattcaaat atgcgattag atctgctgaa aaagaacaaa
      421 aaattcaact gaaaattatc tatgaaattt gtaataacaa gtattttgta gatcatgtta
      481 gagaacagct atcaatgact ttacgtgact tgatacgaag agcaaaaaca gatcatcgta
      541 tcaaacaaaa agacgattat atctttgctt ctctaaaaaa taaagcgtta gaactaattg
      601 aattgcaaat cagcgaagga atagataagc aataagaata gaattgagtc aaatgaaatg
      661 gaggggtatc tccacttcat ttgagaactt tgaatatagg aggggttatt ttgattaaaa
      721 atcgtttaat agatcaagtt tggtgtctag atcaaaatac agatagtctc gaattaatcg
      781 agactatttg cttcaacact atagttctcg atttaagagc tgaaaatgat aattgtgtaa
      841 ctcatgtcat aattaatcaa aaaatggctc agaagctctc tgagagcctc agaaaatggg
      901 ctaagggagg taacgatgaa aataattgaa aaaaagattg ccaataaaaa aataaattgg
      961 ttggttgtta attttcttgt tctgatagct atgattattt ttacaagtaa tattgctcat
     1021 gcgagtattt tcaattggtt tgatgacaca aaaagtcaaa cagagttttt gaataattct
     1081 ctttatagac cttacctaaa aaagtacaat ggagatttcc ttagtcagtt cgggatttgg
     1141 ctcggatggg ctgtggtaaa aggcatgttt actgtgacag attcgattca aaatatgatt
     1201 ccagatgtgc tggatttgtt caatttcatt gaaagtacag ggctaaataa tgtttatcaa
     1261 tcagccgtaa atacaattgt cattggatta atgatccttt cattgatgtt tgttggttac
     1321 aaaatgatta ctggaaaagg aacagtggat ttaaaatcgg ttggaatgaa cgtagtgatg
     1381 tctatagcat taatactttt aatgccaacc atgatttcta gtggtattca atttgctaaa
     1441 acattttata aggactctac tacgattaca aattcagatg acggagtcgc ttggtcattg
     1501 ataaaacaag gagtaacaga tttagcatat attaataaaa cagatcagta ctcaagcatt
     1561 gataaaacgg aggatcgaaa taatttaaca cgaaaaaatt ttcaacagac ggatctcaca
     1621 caagtgttaa cggataaagt gattgataaa ttggaaaaag aaaatccaat tgcggacaat
     1681 cttcgttatg aattagttga aaattctaat aatgaatttg ttgctacaaa gttttctgac
     1741 aacttcttat caacattttc tgatagcttg aaatcaggct attaccgata ccaagcaaat
     1801 ctatggggga tttcaattgg gttgacagcg ttagcaatcg cctatgtgtt cagcgcgttt
     1861 gtgatcatta cagcaatact agaacttgca tttaaacgtg tgttaggtgt tcttgttttt
     1921 gcaacagata ttgagacagg acagcgttca aaagttgttc tatcagatat tctacaatgt
     1981 tacttaactg ttgggtttca aggatttggg ttatcaatgt ttgctatgtt tatcaatttt
     2041 ctgaactcag gacaaggtat ctcaacaaat atttttataa aaacaattgc ttatatctgt
     2101 gccgtttttg ttttaatcaa aggcagtgga acagtcatga gatactttgg tgttgatatt
     2161 ggactcaaag aaggctatgg gcaacttgct tctgcttttg gaatgggcgc aatgctgttt
     2221 agaaaaggat caaatggatt taatcgtgct aaaggaagcg gagaaggaga agatcgaaaa
     2281 ccagaaaaga atttcggcga aactctatca aaaaaagctg gcaaaactgg aagagctttg
     2341 ggatatgccc atgaacgtgg attatccggt ttagcttcag atggagctac aatggctagt
     2401 gaaagagcaa caaaaccatt taagagtatg cgtgatatag ctaatgatac caagaataag
     2461 tttaaagaag gtttagatga tggtacagtg tccgctatta ataaaaatag caaacctatg
     2521 ttagctaaaa ataaagaaga tgaaactgga aaatatgctg attccgtgcc aacacgtctt
     2581 tcagatagaa aagatggtgc taaagttgaa aatgccgata gaattatgtc tagttctgaa
     2641 cgtatgcgtg aagcaatgaa aaataatgct gaatcgaata ataatcctgc aagcgcaatt
     2701 cagcaaaaag tccaacaaga tattgaagaa cgtaaaaatg ctatgcacgg tcaagctaag
     2761 tctgctgaag aattaattaa tcagaagcgt catgaagcga aatacacgcc tgaagcaatg
     2821 aatcgcgagg aaataatgtt aaggaaacgt gtagaagggc ggacaggcgc taatattgac
     2881 ggaaaagaag cgttgaccaa agaaagaatt caagaagcta aaaattcaaa tacaggatta
     2941 gaaaaattag tcaaagaaaa tccacaaaat tctacaagta gtcatggtgg ccgatcagta
     3001 gatgtccgag aaaatattca aggttcactg aatggtcatg ttggacggaa tgttgatgta
     3061 agagagaatt tacaaagttc ttcaaatgct caaggtacta agacaattga tgttcgagaa
     3121 aatctgcaaa gtagcagtac aagtcaacca aaaacagtag atgttcgaga aaatgtacaa
     3181 aattctacaa gtagtcaagg gtttaaatca gtggatgtcc gagagaatgt gcaaggtagc
     3241 agtgcaagtc aaccaaaaac agtagatgtc cgagaaaatg tacaaagttc tacaagtagt
     3301 caaggattca aaacagtaga tgttcgggag aacattcaaa aagatagtgg agaaatgaaa
     3361 gaaaaaacac aaaaaatcaa tatcgttgaa gacagaaagt cagcgaaaaa atttgattca
     3421 aatcatgaaa cagtgattat tgatagtgaa atccgtgaaa atgataaggg atcaaaatca
     3481 agacgaagat tcacttatga agacaatgag ttattcagag atacacttaa tgatcctaat
     3541 ccacttttcg ataatttatt aagaaataaa agggggggat tgtcatgcaa ttaattctaa
     3601 agattagtac gcatgatttc ttagaacttg acgaattatg tgaatcagta aagaacgcaa
     3661 caaatgaaaa aatcacggtc actaattata ttgaactgat gattcaagaa gaattaaaga
     3721 tgaaaaatga aatagagagc aatttcaaat gttgctctct agaaaaaaat gtgaggggat
     3781 aatcaatgga tgataaacaa aaaatgtacg aaaaaaatcg caaacatagt gaggccttaa
     3841 attttcgtgt cgataaaaag acttatcttg aactgacatt gattgcaaaa caaaggggaa
     3901 tttctagagc agacgtttgc cgtgagcggt taaagggact caaatatgaa ccgccgttgt
     3961 tagctgaaga tgatagggta gttttaacca aacagttaaa tcaaattggt aacaatttga
     4021 atcaagcggt aaaggcatta aatatcattg ctaaatattt tagagaagaa tctgaagata
     4081 aaaaagaaaa gagcctacat tttagttttg atgctagccg ttacaacatt tttaaagatg
     4141 aacttgcgaa tagcatagca aaaaagttga tgattagtta tccagatgac atttttttgg
     4201 agtccaatcg gagtgccccc tcttttttta aattatcaga agtagaaaaa gaatatttcg
     4261 aatattggat agaaaagcaa actagaccgg gtacgaaaga agagtattcc caaaaagttg
     4321 atcggaattt aattcactca attcagatta ttgcaagttt aaaagatgag gtgaaacaac
     4381 tttgggagct actataacat cgaaaggacg caagaaaaat ttatcctcgc tattacaata
     4441 tgcagctaat ccttcaaaaa aagaaaaatg tgttgcagtc tcaggaataa attgtagtga
     4501 tgatagtgag gaagcactgc aagaaatgaa tttagtacgt accaagatga ataaatctaa
     4561 agatatagca caagcgtttc atgttattca ttcgtttgat aagagtactt ctaaagagtt
     4621 aagtattgat aaaatgcacg aaatagctgt cgaatttgca gagaaagctt ttccaaattc
     4681 gcaaatcatt gttgcaagtc acaatgataa agatcatttt catagtcatc ttgtcattaa
     4741 taatattaac atggaaacat gtaaaaggtt gcgtattgac cctaaagatt tagaactaat
     4801 gcgaaaaatt aacgatcaag tcctcaatga acatggtctt gaatcagtga ataatgagta
     4861 ctatgaaaac ttagaaaaga gaaccaatct ttattcaaac gatccttcta agatgaaata
     4921 tggaaaaatt aattatcaag agtttgtgca aaatgcgatc gaagcaacgt tggaaaaatg
     4981 cgaagtgaat tcttttgaag atttttcgga tcatttggca aaagaatata acattgaagt
     5041 ttatcaattt tcaaaaacaa ataacaaact tggctatgtt ctttacaaac aaggtgtaaa
     5101 tttctatgag cgtagaggtg agttaattac tttaggaaga gataaacaat ctagaaaaga
     5161 aaaaagtcag tatgtcgaac gtacttttag tgcaagaaag ttagggaaaa gatattcatt
     5221 tgaatctgtt tctgaacaac ttgaaaagaa tttagaagaa tcacatataa aaaatttttt
     5281 taatatggaa agagagcaag ttatgtcgct aattagtgga gtaaattaca aaactggtga
     5341 gttaaatgta ccgtttgtgg aattagattc catcgcacaa aagcaattta gcgaccgtga
     5401 aggcgatgaa aaattcatta aatatgatga cttcttgcga gaggtagaca agcattttgt
     5461 aaatacaatg tctttagaag atgatcaaga atacacgatt aaacttcata cggatgtgaa
     5521 tcctcaaact ttacgtagtg cgaaaagtaa aacaatttct attaaaaaga ctgatcagaa
     5581 agagttattt ctaccggcaa agggttttga aaattgttct catacacctt ctgctaaaca
     5641 gattcactat caaatcagtc gcataatgaa tgacgatgta aaaatgtatc gtgctaaaaa
     5701 ggattatgaa ttcacacaac aaatgattga aaatcagcaa aaaaggcaac aaaattttga
     5761 aatggaattc tgaatgggct tgttagtgga ctttccagtt ttttgcctat gaagagttgc
     5821 ttggaaagaa gcagttttta ggacatacgc cagctaccca tctacttgcc agtcaatccg
     5881 ccccgctcac gctaggctac tttctggcaa gtcagatggc gggtaaatct ctgagggagc
     5941 aagctcccta cagactccct cccaaaaaaa gaaaggtgga agaaaaaaat gaactatcca
     6001 tattttaaag tctcggcttc agaagaaact aaagaaatat tcaacaattt ttacaaccaa
     6061 aacaaaggaa tttttggttc aaaggcaaat atgtttcgtg ttatggtttc aaatttacca
     6121 gtgttagcta gtccatcaaa taataaattt aatgatcctg aaagcattaa atttgaacaa
     6181 aaaatttcag aactagaatc aatgataagt aatgaagtaa ttgaaaagct ggatgacatt
     6241 gatcaaaagt tatcgtattc tctaaaaaac aaatacaaaa cggaggagaa aaaagatgtt
     6301 taaagtttac ttcccgtttc agtggctcgt taaaggaatt cttttagtgc tactaggatt
     6361 gattcttttt ggaagttatc taacaattaa agccagacat tttccagaag aaaagccaat
     6421 aaaaattgta acaaaagatt taaaaatagg tgaaacttat caggcagatg gggttgaact
     6481 aacatttaaa aatattgaag tgaccgaaaa aagaaatgaa tttgcacaac atgatcgtgt
     6541 attacaagtg acctatacac ttataaatga tacagaaaat ccgatacgtc cacaacaatg
     6601 gtcgattgaa aataaggatg gtgaagaact ttatgagtat gatatgatat tggataatcc
     6661 agagtatatt gaaagtggca aagaagaaac agtaaattac tattatgaat taccgattga
     6721 aaggcaggac taccaaatcg tagtacgtag agagaatggt tacatagttg ggttgccatt
     6781 tactttataa gttttatttt gttagtagtt tttaaggtaa tatcagtttt gtgatttttg
     6841 aaaatggaac tgggagaatg gctgaggaaa ataaagttgt tccgtttgaa acattagtga
     6901 taaataagga agagtttgag caactttttc ttgatttttt tattcaatat gacatgattt
     6961 atgattttag ttcgtcacta gatttatttg aattagaagc agaaataata gaaaacagtc
     7021 gagagaagaa tggtaaaatc attatttcag tagaaaaatt caatgaatta ttctcacaaa
     7081 catttgaaat agatccagac tattatcaac cacaagaagt ttttcgagag tttagaaata
     7141 agattattgg ttttagtcaa actacaaaat gaaagcttaa taaagcaagt gttgatagaa
     7201 ttattcctca acgcttgctt tattttatgg gcacttcata atagaggtat agagaagaga
     7261 ggtattcatt ttggatacta ttactccaac aaacttcaaa gaaaatatag gtaaagtgtt
     7321 gcaagaagta gttcattcaa attctgaaat agaaattact agggattcta aaaatggatt
     7381 gaacgatggc gtcattatga tttctaagag aaaatgggag ttgttacaag aagaacttta
     7441 tttacaacga acgggtacat gggcttatgt atctgattta
//
LOCUS       DAZKOR010000107         7389 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000107 DAZKOR010000000
VERSION     DAZKOR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.110"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            407..676
                     /gene="rpsN"
                     /locus_tag="ACG8ZM_002460"
     CDS             407..676
                     /gene="rpsN"
                     /locus_tag="ACG8ZM_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0493388.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            691..870
                     /gene="rpmF"
                     /locus_tag="ACG8ZM_002461"
     CDS             691..870
                     /gene="rpmF"
                     /locus_tag="ACG8ZM_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0493389.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            902..1279
                     /locus_tag="ACG8ZM_002462"
     CDS             902..1279
                     /locus_tag="ACG8ZM_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493390.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1300..1449
                     /gene="rpmG"
                     /locus_tag="ACG8ZM_002463"
     CDS             1300..1449
                     /gene="rpmG"
                     /locus_tag="ACG8ZM_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0493391.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1473..2456
                     /locus_tag="ACG8ZM_002464"
     CDS             1473..2456
                     /locus_tag="ACG8ZM_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0493392.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2486..2608
                     /locus_tag="ACG8ZM_002465"
     CDS             2486..2608
                     /locus_tag="ACG8ZM_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0493393.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            2626..4149
                     /locus_tag="ACG8ZM_002466"
     CDS             2626..4149
                     /locus_tag="ACG8ZM_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0493394.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            4173..4412
                     /locus_tag="ACG8ZM_002467"
     CDS             4173..4412
                     /locus_tag="ACG8ZM_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493395.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4605..5168)
                     /locus_tag="ACG8ZM_002468"
     CDS             complement(4605..5168)
                     /locus_tag="ACG8ZM_002468"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0493396.1"
                     /translation="MDFSISFITIYRAICNDLFDTEPLSHGNKGLIRSLHHRGKTRHT
                     KNHVESRGKICLTHSIHGHPKSADDRSDFGHWKADIVAGKTSGTCLVTLTDRKSCFLL
                     AGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITPDRGKEFSNHFAVTEVLNNMPFYFP
                     DPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5261..5479)
                     /locus_tag="ACG8ZM_002469"
     CDS             complement(5261..5479)
                     /locus_tag="ACG8ZM_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493397.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            5668..6615
                     /locus_tag="ACG8ZM_002470"
     CDS             5668..6615
                     /locus_tag="ACG8ZM_002470"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0493398.1"
                     /translation="MYGDHGKEIAVIVNDIGEVNVDGVLVDKKGFKRTGEKLVSMPNG
                     CICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPIIQAIILAKNPLDIDLTQRL
                     EIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDIRGLLIDQFECCNILLLNN
                     SDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILNTNLFNIDETSYSMGWFKE
                     LELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKNFPGNITRAKGFLWFAQHS
                     DFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            6582..6671
                     /locus_tag="ACG8ZM_002471"
                     /pseudo
     CDS             6582..6671
                     /locus_tag="ACG8ZM_002471"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6804..7211
                     /locus_tag="ACG8ZM_002472"
     CDS             6804..7211
                     /locus_tag="ACG8ZM_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0493399.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
BASE COUNT         2615 a         1094 c         1443 g         2237 t
ORIGIN      
        1 tgaattagtt cacttaatgt ctattcttat aatataagga ataatcaaag ttttaaaaaa
       61 ttggcgataa accagttaaa cataaaagat tgaataaaaa gttatagttt ctttcatatt
      121 gccgttttaa gtagaggaaa agctacttaa aacggtattt gtgtttgatt ggactaataa
      181 aagtgaattc ttatttttca gagcttaaac tgcatattct tttaaatgat ttgattatct
      241 ctatgtaccg ataaattcgt tttcaaatag ttatctattc atatgagttt tatttcatgt
      301 tatttattgt tgacaagagc aactcttgtt ggtaaagtat cagtgtcaaa acgtaacgat
      361 tacgatttga gatgtctaaa tcaaaataaa aggagtataa aggtatatgg caaaaaagtc
      421 gaaaattgct aaagcaaaga aacaaatggc gatgattgaa aagtacgcag ataaacgtca
      481 ggaattaaaa gccgcaggag atcgcaccgc tctcgctaaa ttgccaagag attcaaatcc
      541 gaatcgtctg aggcttcgtg atcagacgga tggtcgtcca agagggtata tgcgaaagtt
      601 cggtatgtcc cgtattaagt ttagagaact tgcccatcaa gggttgattc ctggagtaaa
      661 aaaagcaagt tggtaaaagg aggaaatatc atggcagtac cagcaagaaa aacatctaag
      721 gcgaagaaac ggttaagaag aactcatcaa actgtagtaa aaccagaaat ttcatttgac
      781 gaagcgaatg gtgattatag acgtagccat catgtatctt taaaagggta ctataatgga
      841 aagaaagtaa ttaaaggagc ttcaaaatag tttaatatat aacttttagg agggaatcaa
      901 aatggaaatt atttacccac ctttagttga agaaggatta aagtattatc ttgaaacgac
      961 ccagcaatcg ctagataaat gcacttttta tcgttcgatg gtagagagag gaattattac
     1021 tgaaacgggt ttaccgaccc aacaggcaat tgaaaatgga ttagtcaaag attactatga
     1081 ggaccaaggc ttatcatttg atgatttttt aagaatctat ccaatttttg aagagtatga
     1141 cgaggagtta tttcaatgta ttgatggtta ttgggagata cctgttgata tgaaagaaaa
     1201 tttagtttca caattggaat ctggcgagtt aacttttgaa gatgcacaac aaattcaagc
     1261 atatcttgaa gatcgataag tattgaagga ggaagattta tgcgtcaaaa tattattttg
     1321 gaatgtgtcg aaactggaga acgtctgtat ctcaccagca aaaataaacg gaataaccct
     1381 gaacggttag aattaaaaaa gtattcgcca aaattgcgca gacgggcaat tttcaaagaa
     1441 gtgaaatagc taagtggtaa aaaggtgaca aaatgggcat accagttaca atcgtttctg
     1501 ggtttttagg ggctggaaaa acaacgctaa tcaatcaagc cttgcaagtc tcaccctacc
     1561 ccagggaaga aattattata attgaaaatg agtttggtga agttgggatt gatcacaaat
     1621 tattagttca tagcgaggaa cgggtgttac aactaaataa tggatgtatg tgttgcagtc
     1681 ttcgcagtga tttattagca atattgattt ctttactgga aacaatagaa gaacatcaac
     1741 aaccaatttc acaaatcatc attgaaacta caggtatagc agatccccaa ccaatcattc
     1801 aaacactttt aacagcgcct caactaagag gaagagtata tatcgatagt ttattgactg
     1861 ttattgatag tgacagtttt gaacttattg aaagagaacc acaagccctt aagcaacttg
     1921 tcatagcaga tcgcattttt gtgtctagaa aacaaaactt gaatttatct gcactaaaaa
     1981 aattacagag agaaatcaaa agtattaacc ctttatccga tattcgaatg ttcacacata
     2041 gtgaaacgat tcaaaaaaag gatttttata atttggaaaa attcaatcat cccttagttt
     2101 ttgaaagtga ttcatcagaa aatcactata atggcaaaca ctatcatcaa catcatcaag
     2161 aacatgagtt taaatcaatt ctcttaactt cagatcaaga cataaaaaaa gattttttat
     2221 tccaattaat tgaccggctg attttaacca accaagggag cctgtatcgt tttaaaggat
     2281 tgattgcttt gcaagatcaa gattttatag ttgccataca aggggtaaac gaacaggtta
     2341 attttcaata caccactcga caaaaagacc aaaccactat tattctaatt ggaaaaatgt
     2401 tggatgaaga aaggatcaaa cagagttttg ataaattact ggcggaaagc aaataatgat
     2461 aagtattgat taggggtaat ttctaatgga gaaaacggat atatctagtg catatcgacg
     2521 acttaaaagc ccgaatatca aaactagaaa aagggcatta aaaattatca aagaacataa
     2581 acaaaataaa atgaagaaat tagcataatt aaggaaggaa aaagaatgaa gaaaatcagt
     2641 gtaggactta ttgggatagc agctctagga ttattgggag cttgctccag cactaatgat
     2701 gcaaaagtat cgaatgataa agatggaaaa ttagaaattg taacaacatt ctatccaatg
     2761 tatgatttca ccaaaaacat cgtcggcgat gaagcgaacg tggatttaat ggttccagcg
     2821 ggatcagaac ctcatgacta tgagccctca gctaaggata tggcaaaagc tcatgatgct
     2881 gatgtttttg tatatcataa cgaaaacatg gaatcatggg taccaaaagc taaagaaagt
     2941 tggaagaaag ctgggcctaa tgtagtagaa ggaacaaaag acatgatttt attgccaggt
     3001 agtgaagaag aagatcatga tcacggagaa gaagatcacc atcatgaact agatcctcat
     3061 acgtgggttt cacccaaaat ggcaattaag gaagtttcaa atatcaagga tcagttagtt
     3121 aaattgtatc cgaaaaaagc aaaagtattt gagacaaatg ctgaaaagta tttgactaaa
     3181 ctgaaacggt tggatgctga ctataccact agtcttaaag aagcgaaaca aaaaagtttt
     3241 gtgacacaac atgcagcctt tggatattta gctttggatt atggattgat acaagttcct
     3301 attgctggac taagtcctga agaagagcca tcttctggtc gattagctga attaaaagag
     3361 tatgttaaaa aaaataagat caattatatt tactttgaaa agaatgctaa tgataaaatc
     3421 gcaaaaactt tggccaatga agctgggata aaattggaag tattgaatcc tcttgaaagt
     3481 cttacaaaag aacaaatgga taatggtgaa gattatgttt cagtaatgga agacaatcta
     3541 aaagcgttag agaaaacaac tatggttgct ggtgaagagg tagttccaga aaaagaagcg
     3601 aaggatgaaa agacagttgc taatgggtac tttaaagatg ccgatgtgaa ggatcgcgaa
     3661 ttgagtgatt atacaggcga atggcaatct gtttatccgt tgttaaaaga tggaattttg
     3721 gatgaagttt tcgattacaa agcaaaactc aataaagaca tgacagctgc cgagtacaaa
     3781 gattattata ctactggcta taagacagat attgatacta tcaacattaa agacaacacc
     3841 attgattttg tagtcaacgg tgaacatcat caatacacat acaaatatgt tggatacaaa
     3901 attttgaatt atgaaaaagg aaaccgtggt gttcgtttta attttgaaac tgatgatgca
     3961 ggtgctggtc gttttaaata tattcaattt agcgatcatg gaattgcacc aagtaaggca
     4021 gagcatttcc atattttctt tggtggagaa agtcaagaaa aactttataa tgaaatgcat
     4081 aattggccta ctttctatcc agcttcatta tctgagcatg agattgctca agaaatgatg
     4141 gcccattaaa atccaatctt tggaggaaat acatggtaag aaaagtatta ttgtctatgg
     4201 attccaagaa tattgatgct tatcggcgtt atcaatttgc aaagcaaatg agattaaatg
     4261 ggagtacatt agctgatatt gctgcaactt tacatgttag tattgacaca gttcatagag
     4321 tattaaaacg ggatcctgat tgttgtttta tcaaggtcaa tactggaaga agtagacgtt
     4381 tagcaaaacg gcataatagc ttggccaatt aattgataat accagagtag atcatttttc
     4441 ccttatttgg atttgatctg ctttttatta tttcttgaat tgtcaaatca agcgcaacac
     4501 tttatcaaag aagacttcat aaggtgttct ccaatctaaa cactttctag gtctgttatt
     4561 cagtttttca atcatctgat ttatcgttga gttatctacc gtagctatct ctttcgattt
     4621 tggcaagtat tctcgaatga gtctattcgt attttaattt gttcattgtt gccaagggca
     4681 tgaggatctg gaaaataaaa gggcatatta ttcaatactt ctgtaactgc gaaatgattg
     4741 gagaattctt ttcctctatc tggagtgatc gttttgaccg cctcatttgg aatactgctt
     4801 aaaagttcaa tcattttctc gctcacataa gcggctttct ttctggggac cttctttcct
     4861 gctaaaagga agcatgattt tctgtctgtc aaagtcacta aacaagtacc actagttttg
     4921 ccagctacaa tatcggcttt ccaatgacca aaatcacttc tatcatctgc ggattttgga
     4981 tggccatgga tcgaatgagt aaggcaaatc ttcccacgag actcgacatg attttttgta
     5041 tgtcttgttt ttcctcgatg gtgcaatgaa cggatcaggc ctttattgcc gtgtgagagg
     5101 ggttctgtat cgaaaagatc attgcaaatt gcacggtaga tcgtgataaa actaattgaa
     5161 aagtccatat gttcatgctt caaacgattc gagattttct ctggcgacca ctgaaaatcg
     5221 acaaataaat gacgaacaat tgaccaaact cacggatcat ttagaatgtg ataacgtccg
     5281 catttttttc ttcgataagc ataatttttt tccgctttgg aaggtgatta gtttttttct
     5341 tgtttcttct cagttcatgt aaaatagtaa acgggctatg ctgaatgtcg ttgccaatta
     5401 tactaataga aaatccttct tcgtgaagca gaaagattct ttctcgtttg agtatagtaa
     5461 gatgacaata ctggctcata attattttcc ttctaggagt gttctcgaca aacttatact
     5521 agttggatat cgattatgac tcatttttat gtgttgcact taaagtgtaa attcaaggta
     5581 tagaaagagg ttccctatga aaaaatacct attacaatct taactggcta tcttggttca
     5641 ggaaaaacga tacttctaaa tcatattctg tacggtgatc acggaaagga aattgctgtc
     5701 attgtaaatg acatagggga agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt
     5761 aaaagaactg gagaaaaact tgtttcgatg ccgaatggat gcatttgttg tacattacgc
     5821 gaagatttga ttgttgaact agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt
     5881 attgaagctt caggaatcag caaaccaatt ccaattattc aagcaattat attagctaaa
     5941 aaccctttag acattgattt gacccaacga ttagagattg gtgccattat tatagtagtg
     6001 gatgtaaaac gtttttggga taatttcgaa acgggtgaaa agattcagga aagaaaagct
     6061 gaaggtaatg tagttgaaga agaacaggat attcgtggct tgttaattga tcaattcgag
     6121 tgttgtaaca tattactttt aaataatagt gacttagtgt caaccaagac gatttacaaa
     6181 attaacggtc taattgctaa gttacagtcc aatgccaaaa taattaggac aattaaaggt
     6241 gaagtagact tatcgaaaat tctaaataca aatcttttta atatagatga gacttcctat
     6301 tcaatgggct ggttcaaaga gttagagctt ggctatcaaa atcatattcc tgaaacagaa
     6361 gaatatggta tctcaagttt tatctatcga aatcggttac tccttgacgc acaaaaattt
     6421 gcccatctta cggataaaaa ttttcctggc aatattacaa gagcaaaagg gtttctttgg
     6481 tttgcccaac atagtgattt ttcgacgatc tttgaaacat cgggtagcca taaaagtatt
     6541 gaatcattaa attattgggt agctagttta cctaaaattg agtgtcaaat gattatacaa
     6601 aaaaacccga attgaaggaa gcctggcatc cgttgtttgg tgaacgcatt aataaactag
     6661 tttttattgg tatgaagatg gatcaacaat gtattaagga acaacttgag ggttgtttaa
     6721 ctgattctct taatgcagat aatttccgaa aaatttcacc atttccatgg aaaaattcta
     6781 atatgtttag ccagatatcg ttaatgaaat ttttaagaaa agctggtgtc aagataggcg
     6841 atgtcataat tattggcatt ttgattattc cctcttttat tcccttattt gtattctcta
     6901 tagaaaagga gaatgaaaca actgaagatg ctatcaaata tgccatcgtt caaattgatg
     6961 gcaaggaaat ggatcgcttc gatttggata agatcaatca taaactcgtg aattatcatc
     7021 ctgcgaaggg acaatataat attgttgaga ttaaagatgg aagaattcga gttaaagaag
     7081 ataatagtcc tgatcaaatc gcggttagaa ctggatggat ctcaaaaccc ggtcaaacga
     7141 gtatttgttt accccataag ttagttatta gtattgagaa aaaagaaagc aaagattact
     7201 acatttacta atctggaaat agatgaggag gtttttaata agaaaaagat attattatta
     7261 ataatagcag gggctttatt aagtgtgtta agcgcttgtg gacaagaaaa agaagcggtg
     7321 acaacaggca caagtatctt taacgggacc tgcaatggtt tagttaacag tcggttagat
     7381 tacttattt
//
LOCUS       DAZKOR010000108         7145 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000108 DAZKOR010000000
VERSION     DAZKOR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.111"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..613
                     /locus_tag="ACG8ZM_002473"
     CDS             <1..613
                     /locus_tag="ACG8ZM_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0493400.1"
                     /translation="GEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEE
                     EFGHLEGDTIVGIHHKSAIITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNL
                     FKSITFDCGKEFSNWKSISNTNDIDIYFANPGTPSQRGLNEHSNGLLRKNGLPKEMDF
                     NQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(681..1454)
                     /locus_tag="ACG8ZM_002474"
                     /pseudo
     CDS             complement(681..1454)
                     /locus_tag="ACG8ZM_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1490..2677
                     /locus_tag="ACG8ZM_002475"
     CDS             1490..2677
                     /locus_tag="ACG8ZM_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HFQ0493401.1"
                     /translation="MQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNTLYLDK
                     SLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAKGLFVE
                     KLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYESAMIQ
                     GFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDYQLPEG
                     SRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEMILNDV
                     NNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTEVNINE
                     YSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVTEYIKN
                     YFN"
     gene            3410..4378
                     /locus_tag="ACG8ZM_002476"
     CDS             3410..4378
                     /locus_tag="ACG8ZM_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0493402.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            4446..5204
                     /locus_tag="ACG8ZM_002477"
     CDS             4446..5204
                     /locus_tag="ACG8ZM_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0493403.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKKIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            5272..5982
                     /locus_tag="ACG8ZM_002478"
     CDS             5272..5982
                     /locus_tag="ACG8ZM_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0493404.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            6018..>7145
                     /locus_tag="ACG8ZM_002479"
     CDS             6018..>7145
                     /locus_tag="ACG8ZM_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0493405.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         2432 a         1115 c         1439 g         2159 t
ORIGIN      
        1 gggagaattc aatgttttac atttaccgat gcaaggaaaa cgaaaaccaa atggttataa
       61 ggaaaaacgt ggaaaacagg cattcaaaag aaatatttct gaacgtaaaa aagattatgt
      121 cgttttcgaa gaagaatttg gacatttaga gggtgatacg attgtcggca tccaccataa
      181 aagtgccatc atcacactcg ttgagcgact ttcaaaagcc attatcgcct tgaaaccaga
      241 aggccgtaag gcagttgata ttgaaaattc gattaatgaa tggcttcaat ccgtacccaa
      301 aaatcttttc aaatcaatta cctttgattg tggaaaagaa ttctctaatt ggaaaagtat
      361 tagtaatacg aatgatattg atatttattt cgcaaaccca ggaacgcctt cccaacgggg
      421 attaaatgaa cattcaaacg gactcctccg aaaaaatgga ctaccaaaag aaatggactt
      481 caaccaagtc aatcaaggat tcatctcatc cgttgcgtcc aaaagaaacc atatccctag
      541 aaaatcacta aattaccaaa caccattaga agtttttttg agttacgtaa atggaaagtt
      601 ttgtctcgct taatttgaca aataataagc caataaaact tagtccgata tgcggcgcgt
      661 tctgtgcgcc gcatattttt ttagttttca aagatcagac cttgttgtaa atagatccgt
      721 gcccgaaagt tataaaagtt gcgataacca aacgcaattc gtttaatcag cttgatcttg
      781 ttatacagcc cctcagtgac gccgtttgag tgggacacct taaaggcatt tgaaataccc
      841 gattggtagc gatggaagat cgtgaatttc tttttaaacg actgaggtaa cgtagatgaa
      901 attgttttaa gggtttcttg aaagcctacc gtatctcgtt taacgaaata atagcgtaat
      961 aattgacaaa aatgatacgc agttcgcaat tcttcgttgt agcttaacag ctcattaacg
     1021 atttctgttt gactaattgg tcgtttaaac agtcgatgat aatgccgttt gagtgcctcc
     1081 catgtcgtgg aatctttgag taagagtttc caatatcgct tgagccgacg gtacttcttc
     1141 tgattttcag agtgtgaggt tcgaaaacga ttcatgatct ggacacgtaa ttgattgaag
     1201 ctgcgattga tgtgttggac aatatgaaag cggtcagtta cgatctccgc atttggaaag
     1261 accgttttaa gcaactgaca atagctggca ttcatgtcca tcactaggta cttgaccttt
     1321 aaacgggcct ttctaggaaa gcgcaggaag tattgagtta gtttgtaaag tctgcgatct
     1381 tcaagggtcg taaatagctg attggttgtc ccatcaacac aaataaaact catggaacca
     1441 gaagtggcaa cattgtgtac ttttctttgc aatcttgtgt acaaaactat tgcaaaacac
     1501 agcgcataag aatccttaca aaacagtaga tgaagcaacc caattagatc ttgttatagt
     1561 tgttaatcag ctcttaacgg gttacgattc aaaatgggtc aacaccctat acttagataa
     1621 gtcacttgaa tatcagaaca taattcaggc tttttcacgc acaaatcgtc ttaatggtcc
     1681 tgaaaaaccg ttcggaacca ttcgttatta tcgtaaagca catacgatgg aacagaacat
     1741 tgaagaggct attgagttat actcaggcaa acaagcaaaa gggttatttg ttgaaaaatt
     1801 atataataac ttagaaaagt tgaacgatat attcgaagaa attaagtccg tatttaatat
     1861 ttctggcata gaaaacttca gtcatttacc ggaggttcca ggggaaatta agcaatttgt
     1921 aaatctttat aatcaactaa tccattgtta tgaatcagcg atgatacagg gattcacatg
     1981 ggcaaaatcg acttatgaaa tccaggatta tgaacctata acagtagtaa tcacagaata
     2041 tgaatttaat atcttggcac aacgttacag cgagatagag agagaaccta gagacggaaa
     2101 tgatagtata gcgtatgaca ttgattacca attacctgaa ggtagtcgta gtcgtattaa
     2161 ttatgagtat ttaaacacaa acttcaaaaa atacatgaat ataaaagaag aacgtgattc
     2221 gaatttaatt caatcattgt taaacgactt acatcgcaca tttgcgcaat taccagtgga
     2281 gttacagcca tatgctgaga tgattctaaa tgatgtaaat aatggggaat tagttatcaa
     2341 agaaggatgg gaatttactg attacctcaa tgaatatcag cttagtgaag aggacgattt
     2401 tatccaagat ttagtggatt caaccaatat taatgaggca ttattacgtg agatgttgga
     2461 tttacgctta accgaggtaa acattaatga atatagtcga tttgataaat taaaatcaag
     2521 tgttaacgta ggtcatttca gtggatatat cgagaaaaat ttaggtaaaa tggtaatacc
     2581 aatcaaggta aatatgctaa ttgacagatt attgcgtgca tttttattag aagatgtatt
     2641 cgatgttaca gagtacataa agaattactt taactagagt acacctacat cttatagtac
     2701 ataaaaaaat tcatcttttt tagtttaata attcaccaaa aaggtgagta atgccatttt
     2761 tgaaaattga gcttgtttta tgtctacatc tgtatccgta tataatccaa aggattataa
     2821 actcagaaga gtctttattt tttctgaata taactactgt atcaaaaaca atcgattatg
     2881 agacaaaaca aaatgacctc ttttatttaa gaaaggaggt cattttttgt atcataaatt
     2941 gtattaaaat caatgtatca ctttttaatc aattttaaga ccataaattg cgtactaaaa
     3001 gaaattctgc aattcaaaat aaagcatttt aagccatttt caaatttaag tgcacccatc
     3061 tacccgtcaa atcactttat tttccactcc agtagtttcc taattcaact gtgtgcaaaa
     3121 cgatttcttc taaacaaatc aagacaaact tataaacctt catcccagca ccataattat
     3181 ttaaatcata ggcatttttc gaaattagta tatgtactca agtttcactg cagattattt
     3241 atatttttta gagaaataaa tttttcctat tgacaaacac aaagaaagcg gatacaatac
     3301 aattatgtca catgacgtta tatgacgtta tgtaatatct ttacatttaa tcagttactt
     3361 ttattttgga taaaattaac atttttaaaa ttttaaggag gaaatgttga tggatgtagc
     3421 aaaaacggtt aacgcaattg tagaaaaaaa tgatggagag atttctggaa tttatttggt
     3481 ggcttgtggc ggttcgctgg tagatatgta tgtctcagat tattttctta aagccgaagc
     3541 atcgaagtgt attacaggct tatatacagc gaacgaattt aaccatgtaa ccccaaagca
     3601 attagatgat aaatctgttg tcattctatg ttctcatgga ggaaatacag cagaaacggt
     3661 aaaagcagga gaagtcgctc aagctagagg agctgtgact gttggtctga ctcataataa
     3721 aactgcggat ttattaaagg tttctgatca tagtttcctt tacgaatggg gagatgatgc
     3781 aaaaatagtc aataatccta tggccatcat tcttgattta actgtatctt tggtcaatgt
     3841 taccgaaaca tttgaacatt acggtaaatt cagagaaggc atgaaggtta tcgataccgt
     3901 aattgaaaat ggcattaagc aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa
     3961 agatgaaaca atgttttata tcttaggtag tggaccatca tatggacatg cttatggttt
     4021 ttcaatttgc tctttgatgg aaatgcaatg gcttgattca acttctgttc attctggaga
     4081 attcttccat ggaccttttg aagtaacaga caaagatacg aattttattc tcttgatgag
     4141 cattggacgc actcgtgctt tagatgaacg ggcaaaacga ttccttgata aatatgcaga
     4201 aagagtagaa atcgttgatg caaaggaact aggattggat atgatatcag atgaagtttc
     4261 tgaattcttc aatcccattt tattctacag tattctttca gaatatcgtt cagctttagc
     4321 agatgcacgc aatcatgatt tagatgttcg tcgctatatg gggaaagtgg agtactagga
     4381 ggattaaaat gaaggtatta ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg
     4441 gaattatgta tcctggagga aattcattga attttgcagt ttttgcaaaa aaactgggac
     4501 atgaaagtag ctttatggga gtttttggct cagatgagga ggcagctcat gttttatcag
     4561 caataaaaaa aataggactg gacaacagtc actctcgctt tgaaaaaggc gagaatggct
     4621 gtgccagagt gaagattgat gagggcgacc ggattttcct tggcagcaat tcgggaggtg
     4681 ttactcgtga atacccgatt cagcttactg aagaagacag agaatattta aaccagtttg
     4741 aattgattca tatgggatta tatagccatg tcaatcatct attagaagag ttacaaaatg
     4801 tttctggaaa gatttcttat gatttttctg acgactttac ggaagaagag gttcagcggg
     4861 caattgataa agttgatttt ggcttctttt ctatagaaga cttttccgat gaagaagtaa
     4921 agcagttttt gatgaagcat ttttgtgagc gaaatgatgt attgattgca acccgaggag
     4981 aaaaaacggc tattggttat gatggagcaa agttttatga agtgaacccc gtgctagaag
     5041 aaccagttga tacaatggca gcgggagatt ctttcttaac agctttccta ctttattttc
     5101 tcgaagggga cgatatagtc accgcgatga aaaaggggaa tgaatttgct gggaagtctt
     5161 gtctggttga cggttcgttc ggctttggta ttcattacga ttgagcttgt tttaaattcc
     5221 tgcatttgat aacctaaaga aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat
     5281 acaaatgcac aattaccatt gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat
     5341 acaggggagt atcccgagaa tacgaaaatt cccgctgaat cagagctgat agaaagttat
     5401 gaggtcagcc gaatcacagt gagaaaagca gtagaagaac tggtagaaga agggtaccta
     5461 cttaaaaaac aagggaaagg aacttttgtc aaccggcata aagtttttcg taaaattgaa
     5521 tatgttatgg gtttcagtga gagttgtaaa tctaatggtt ttacaccgac tagcattgtt
     5581 ttggaacgca agatcattcc agcaactgaa gggatggcaa aaaaactcga tatcgctcaa
     5641 ggtgatgatg ttttatatat ccagagaaag cgaatggcgg ataacatccc cattatgtta
     5701 gaaaacaatt atttttctta tgaaagatat agtttcttaa tggaggaaga gctgactgat
     5761 tctttgtatg aagtacttaa agtccaaggt accttagcca tcaatcctgg ggaaactgca
     5821 cttgaaattg tcacagctga tgaccaaatt gccaaaataa tggaagtagc tgttgggacg
     5881 ccctttttct ttatggatac tttaatcaac gatcaaaaca acataccaat tcatgtaggt
     5941 aatcaatatt acctgggaga agtatatcgt ttttacttat gatttcctgt tttgatttca
     6001 aggagtagat tttatttatg aagcaagtat tgcggtcaaa aatgattttt gatggagagc
     6061 agccaccttt caaaggttat attgtaatca atgaagggat cattgaaaag gtggaaaaca
     6121 actggaatta cagtgatcta ttaaatgaaa acacggaatt aattgcttat gatgatcaat
     6181 tcgttatgcc agcaattcat gataatcatg tatttttcag tggttatttg gctatgaatg
     6241 ctggcataga tttaagcgat acatcttcag ctaacgaggc tgtagagcgt attaaagctg
     6301 taatgaacca taaaaaagca gatgaaccaa tttatgctca tggctgggat gctgccctgt
     6361 ggaaaactga cccatcagaa gagctattga atgaaataaa ccactttggt ccaatcacag
     6421 cgatcgacaa aaatcgcagc cattgttgga tgaatgctat tgctaagcag acttatggtt
     6481 ttacagaaaa agaaaccagt gctgaaagtc gagtattatt aattaaggaa atgctagcaa
     6541 acaaaaagct tttagcagag gtttacgctg aatttgaagc attactgctt tcaaaaggtg
     6601 ttatatcaat gaaagaaatc gtttttgatg acgcagaatt tctttctaat atacctcaga
     6661 ggaaaatact tagtaaccta tatgtgcagg cagtccaaga acctctacag catgaacttc
     6721 tatatgagta taaggaaaaa gttttccctg aaaaggtccg ctttggtgga gtgaagatca
     6781 tggtagatgg tgttgttgcg gatagaagtg gagatattta cggtgtttat cagtcaggag
     6841 tagcttcacc cgagattgac tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag
     6901 tagagattcc ttgctgtttg acaacggaag gagataaagc gggttataaa gctgcgtcaa
     6961 ttcttgtcga atttggaaag cggttacctg aaggcgtttt taattcgatt agtgatttgg
     7021 aaatggttac tcaaaagaca gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat
     7081 atccacaaat tctcggcttg aatcaaacgt tagaagaggc ttacatgtca actgttataa
     7141 aaaat
//
LOCUS       DAZKOR010000109         7060 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000109 DAZKOR010000000
VERSION     DAZKOR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.112"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2671)
                     /locus_tag="ACG8ZM_002480"
     CDS             complement(<1..2671)
                     /locus_tag="ACG8ZM_002480"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0493406.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
     gene            complement(2658..3020)
                     /locus_tag="ACG8ZM_002481"
     CDS             complement(2658..3020)
                     /locus_tag="ACG8ZM_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493407.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFLRTSWQLEYS"
     gene            3239..4459
                     /gene="sstT"
                     /locus_tag="ACG8ZM_002482"
     CDS             3239..4459
                     /gene="sstT"
                     /locus_tag="ACG8ZM_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0493408.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4606..5241
                     /locus_tag="ACG8ZM_002483"
     CDS             4606..5241
                     /locus_tag="ACG8ZM_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0493409.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            5432..6172
                     /gene="truA"
                     /locus_tag="ACG8ZM_002484"
     CDS             5432..6172
                     /gene="truA"
                     /locus_tag="ACG8ZM_002484"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0493410.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6317..>7060)
                     /locus_tag="ACG8ZM_002485"
     CDS             complement(6317..>7060)
                     /locus_tag="ACG8ZM_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493411.1"
                     /translation="DLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSL
                     FIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAV
                     AIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNT
                     VLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYL
                     SIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2269 a         1103 c         1350 g         2338 t
ORIGIN      
        1 ttttttgtgt cgcccatgtt gcactgtact ggttacctgc aacaagatca tattgtatca
       61 gacgataatc ttctagaatt tctttttgtt tctcagttaa atttttttct tggataattt
      121 gactatcctc tgtaacaaag acctgttttc cattaaaacg attagacaag ctttctgtag
      181 gatcaattgt taatgcaggt agatgattag atacttctgt caacaacgca tagtaaggag
      241 ttactttgat attcgcatgc tcaagagcca tagcaccaaa gttattagga gcaacaactt
      301 ctttatttaa cgtagctgct tgttcacgac tatacttgtt gttataaatg aaataatctg
      361 tctggtgctg ttctaatcca tattttttcg aatcattccc actatagatt gcgggtaaat
      421 ggtcaccata aaaaacaaag gtaatcggtt tatcgatttt gtcgatttct tggatgaatt
      481 tttttactgc tgtatcggta tagtgaaggc cttgcatata agtttgtaat tcttttcttc
      541 tattaggaat gacagcagtt ccttcagcag tgtagttcaa ttcactatag taatcatcat
      601 aaggcatatg gttttgcata gtggataatt ggatgaattg tgacgtttct gtattgcttt
      661 gtaaagcttt caaggtttct ttgtatgctg aatcatccga tatgcgtggg ctttgttcta
      721 ttttatccgt ataagttaat ttatccggac tgtctaaata atagaacctg tcaaaaccaa
      781 atttatcaaa cacttgtttt ctgcgataaa gagcagctgt aaaaggatgg atggctattt
      841 tttcatcgaa tagatcggta atatttggac ttattttctg acgctccact agctgggtat
      901 aaggtgtaac taacgaagca gacaggttac tgatactcaa gcctgtcagt ccttcccatt
      961 ccatgttagc tgttccgcca ccataaccga cggataacat caatcctcct gttccttctg
     1021 ttataatgct tttgatgtaa ggaatagggt ctgtaggtac tgtcagatta gggacacgtg
     1081 caggatcact gaagctttca cttaaattga gaacgatcgt ctgattgttt aaccagtcac
     1141 ttcttgtttg gttgatttca tctgcctttg tgttgtactt tttcattatt ccattaattt
     1201 tttctttcga atagccagca ggttcttcca tgattttaat gtcgatattg tttaggaatt
     1261 gaatgactgg tccgttatct tttacggcgc cttgctggtt aaagaaagtc ttgtttactt
     1321 taaataaatt aaacagcaag aaagaaggcg aatttttgtg attaatgaaa aagacacccg
     1381 aaaaacatcc taataataca gcgatactga taaatcgacg tttccaatga atcttcaagg
     1441 aatactgttt ttgtagtcta cgttgtacaa taatactagt aatcaataat aggagaagga
     1501 tcaatccgcc cacgattaat aagactgggc taaccatcga taatagttct gacaatccgg
     1561 tcagtatttt taaatctgaa ggaaagacag gctcttcccg catttccatt ttgattactg
     1621 tcgaaatagt gagcagtaag ttaatgatca aggtagcagt caaggcgtac caaaaacgat
     1681 tggaaatcaa caatagtaag aagaaaaatc ccataataat gatgattgtc aggatcatgg
     1741 gtgcttgtcg ctgagtaaat atttttaagg caacctggat agtttctttc caaccttctt
     1801 ttggttctaa aacgaaaatc tgcacgaagg taaagcagta aaaaaatgct gcaactaaaa
     1861 aataactttt atgaacaaaa agccaatgaa ttcttttttt ccaacgctga gttaattgtt
     1921 caatcgaatc aaaagcgaag tattcgatga aacgatgaat gaaacggatt ttttgtatat
     1981 aaaataaaaa gataactagg agggacactg ctaataaata gcaaataaaa aactgcccga
     2041 tatttatcag ccatgccttc cctgagtttg gaaattcacg tttcataaat gtagatacgc
     2101 gataagaaac taaagggaaa ttacatataa cttgagtgac aatcaaaaat acagcaagaa
     2161 aatttcctct tccttttata cctaatttat cagatagcat ttctaaaaat aaaaacgaag
     2221 agaaagtata atacattgcg aagagagaat atggtttaga aaacctcagc gctgtatctg
     2281 tatttcctct gatgatgagt gaaatttgtg tcatcaaaat aatcaaaccg aataaaacaa
     2341 tcaagctgat aaatacttga ggaagcttat agcggacctt ttctttccag ttccattctt
     2401 tcaaaccata acccattaaa aataaataaa aaatccataa aatactctgt ccattctgta
     2461 atgcaaacgg gtctttatta aatagagtag agcagccaac taatccaatt gttaaaaata
     2521 atagtagata tttaattgtt gttttcgata atttatttaa aaatccaact gtataaggaa
     2581 tactacaaaa aagcaaagta caagaaacag cataagtaaa gtaattctga ctgataggtg
     2641 ttaggataag ccaataatca cgaatattca agctgccagc ttgtacgtaa aaaaaagata
     2701 ttgcaaacta gtgtggctat taggaatata aaggcaaaac gacttaaata atgtaaaact
     2761 tttaaacggg cgattttttg atattggtaa cccatgtaaa gtgggatcaa atttgcacca
     2821 ataacaacgg aagtttgtaa aaatgtatag atgtagaatc gaccagggtt tgttgcatat
     2881 gtaggaatag tgactgctga aatcaatata cgctgtaaaa ccatcattat acaagcgaca
     2941 aagattgtca ggaagccata actctccttc tttatttttt tcaagaataa acacctcaca
     3001 aaaaaattgg acaaaaacat aagaaaacgt ttcctgataa gttgcccatt aataacatcc
     3061 taacttagaa tatctcaaaa aacaacgtgt agacagaaaa agtacaaaat tagcaagaaa
     3121 tggttgtttg tattgttaga atttttagaa aattatacct ttagtttctt tgcataaaat
     3181 gatacaatga ttgttaaaat tgtgaaaggg tggtcatttg ttagagaagt ttttggatat
     3241 gtcgttagtt caacgtattg ttgttggtat tttagtcgga atattgtttg gtatcttttt
     3301 ccccacatgg acatttattt caatattggg aacgttgttt gtcagttgct tgaaggcaat
     3361 cgcacctatt ctggtttttt tcttgacgat ggcgtcaatt gccaagcata aaattgggaa
     3421 taagacattc gtgaagccta ttttgatctt atatttagtg ggaacattgc tttctgcact
     3481 tgtagcagtt attgtaagtt caatttttac tgttcctatt actttacaag aaacagtatc
     3541 tgaaaaagca ccgcaaagtt tggaaagtgt tttatctact atgttgacga atgtcacaca
     3601 aaatcctgtt caatctctta ttgatgcgaa ttaccttggc gtcttattct gggctgtatt
     3661 gttgggacta gcttttcgag cacgttcaga atcgacaaaa gagttggtgg atcagatatc
     3721 tatagcgttg tcacaagtcg tacaagtgat tattgcattt gctccgatag ggattttagg
     3781 attggtttat caatcaattg ctacgactgg aatagctgga ttggcagagt atctgcagct
     3841 tttacttgtt ttgatcggta cgatgctgtt tgtcgcatta gttgtgtacc cattcctcac
     3901 gtttttgttt attaaagaaa atccctatcc attgattttc ttttgtttga aagaaagcgc
     3961 acttcccgct ttctttaccc gaagttctgc cgcaaatatc ccgataaaca tgatgttggc
     4021 tgaaagattg aaattgacaa aagagtctta ttccatatcg atcccgttag gtgctacgat
     4081 caatatgggc ggtgccgcga ttaccatttc cttgatgacg ttgactgctg tacattcttt
     4141 agggattgaa gcaccttttg ttttgaaagt ccttttatgt attttgtctg ctttagctgc
     4201 ttgtggagct tcaggtgttg cgggcggttc actgttattg attccattag catgtagttt
     4261 gtttggtatc tctaatgatg tcgcgatgca aatcgtcggt attggattta ttatcggtgt
     4321 tatccaggat tcaatggaaa ctgccttaaa ttcgtcaagt gatctgttgt ttactgcaat
     4381 aggagagcta ggcgcgcgta aaagaagtgg ggaaaaaatc aaattgaaag attgtttaga
     4441 tggattaagt gaacgttgaa cgttagatat ttagtatttt taaaatgatt cgagttttta
     4501 atgagttata tagatgttcg tgtaaataga ggtttctgtt aaatcaagat aaagtcgctt
     4561 ataataacaa ttaaaagcat aggtaattgt tattggaggc gtatcatgga ttttttacgt
     4621 aaatttggtt ttttcttttt attattaggc gtgctgagtg attttttgac tccgtacgta
     4681 ttaggattgt attatcctga attagatcaa atgaagagcg tgatcagtgt atttggagag
     4741 gtttccagtc ctgtaagaaa agcgtttctt atttggtctg tggtttcagg gatctttttc
     4801 atcctgtcat tgcctgcatt gtatgctgca tttgtaagta catctcgtgt gttagctacc
     4861 ctagttacag cagccatcgc gttctatggt ataggtgatt gtatctttac aggtttgttc
     4921 agtatcaata cccatgaatc ttcgtggaat gtctcgacat gggtccataa tatcggatca
     4981 ggtttaggtt acagtggttt tttattgttt ccagttcttc ttttcttgat ctatcgaaaa
     5041 aatgggcaaa tggacgtaag ccacttatac cttggactgt ctgtgatcag tttgcttttt
     5101 gcggcgattt atggactggc aagaattcct tctataaatt cttggccatt actgaataaa
     5161 ataggttttt gccaaagaat cagttttttc tttaattatg tgcccattgc ctggttgagt
     5221 atcttgcatc tcaaacagta aaacttgagc cgtcaaattg aacagtcagc ggatacttca
     5281 tccggtggct gttttttgtt tttctgaaat atatatttcc acagttttct tgtatagctt
     5341 ctgttttaga gaagaaagga gctatatttt gtgctacaat cttaaaaagg tagagagaag
     5401 accaaatgga agacagagga gccactaaaa tatgagaaac atcaagttga caatcgaata
     5461 tgacggaaaa agatattcag gatggcaaag acttggagat gacgataaaa ccattcaagg
     5521 aaaaatcgaa aaagtattgc atcagatgac aaatgaagag atagaaatta ttggatcagg
     5581 aagaacagat gctggcaccc atgcgcgagg acaagtagca aattttaaaa caaacacaga
     5641 aattggactg gcagaaatga tcgactttat gaatcgttac cttccgcggg atattgtcat
     5701 caaaagaatc gaagaaatgc ctgagcgctt ccattcaaga tataatgcgg tgggcaaaaa
     5761 atacagctat tatgtttgga acaacgtgat tccttcagca tttgagcgaa accacagctt
     5821 ttatttccct caagagttgg atatggacaa gctgaatgca gcttgcgaaa aattagtggg
     5881 tacacatgat ttcattggtt tttctgcatt gaaaaaatca aaaaaatcca cagtgcgtac
     5941 gattgatgaa ttatctatcg aacgaaaggg agaaatgctt cattttactt ttgtcggaga
     6001 cggattctta tataaaatgg tgcggattat catggggaca ttactagaaa tcggcacagg
     6061 tgccatgccg atagaaacaa tcgatgaaat atttgaaagc aaagtgcggc aacatgcagg
     6121 agaaaccgtc cctgcacaag gtctgttttt ggatgaagtt tattatcgat aaaaatactt
     6181 tgataatgga tgaaacgcat gtttcacagt ataaatatcg atgttaaata atgtatgaag
     6241 caaattaaag aaatataaaa aaagactttt tagcctgttt atgtgtacag ctaaaaagtc
     6301 tttcttaaat aaaaatctag tgagaatgtt gtacttctct tctcggttgt ttttgtttag
     6361 aaaaagtaaa tcgataaagt aaaaataaaa tcaaagtaac gatagataaa tatcttgaag
     6421 caattgctag ttttgtgtac tgataagtaa taacgacttt atttatacct ggaggaatag
     6481 ttaattcagt tgtgccaaac ttagataatt ttgtttgtac tagtttgcca ttaatagaag
     6541 ccacttgccc tttataacga taaataggtg tcgtcaagac agtattttta ttattcgaat
     6601 tatctaattc aattgtaagt ttgttactca taaaatgagt tgttggattt atttcctggt
     6661 tacctagtaa atattgatca ttattaatca tatcaggatg atagattgac tctttgtttg
     6721 cataatcagt atgattgtaa ttttcagcta tagcaacagc gtcttcaggt gctataagtg
     6781 ttaatgaatc tttgttagca tggatagaat tgcttactcc tgagagatgc aatattagtg
     6841 caagaaacgt aaagaccatt attttccatg aattattttt agtagaaaaa atataataga
     6901 tcactatact catcgtatag gctataaata aagaagaaaa accatttaac cgccagacaa
     6961 attgtagact cttaactggt gtatggttaa aaagttgcca aggaaataaa ttggttgaac
     7021 aaaaaagaac aaatacacca agaaagaaaa taaataaatc
//
LOCUS       DAZKOR010000110         6909 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000110 DAZKOR010000000
VERSION     DAZKOR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.113"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..218)
                     /locus_tag="ACG8ZM_002486"
     CDS             complement(<1..218)
                     /locus_tag="ACG8ZM_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0493412.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNE"
     gene            complement(837..1169)
                     /gene="traE"
                     /locus_tag="ACG8ZM_002487"
     CDS             complement(837..1169)
                     /gene="traE"
                     /locus_tag="ACG8ZM_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0493413.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(2016..3056)
                     /locus_tag="ACG8ZM_002488"
     CDS             complement(2016..3056)
                     /locus_tag="ACG8ZM_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0493414.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            4222..4308
                     /locus_tag="ACG8ZM_002489"
                     /pseudo
     CDS             4222..4308
                     /locus_tag="ACG8ZM_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            4862..5086
                     /locus_tag="ACG8ZM_002490"
                     /pseudo
     CDS             4862..5086
                     /locus_tag="ACG8ZM_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5346..5600
                     /locus_tag="ACG8ZM_002491"
     CDS             5346..5600
                     /locus_tag="ACG8ZM_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0493415.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            5601..5918
                     /locus_tag="ACG8ZM_002492"
     CDS             5601..5918
                     /locus_tag="ACG8ZM_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0493416.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            6477..>6909
                     /locus_tag="ACG8ZM_002493"
     CDS             6477..>6909
                     /locus_tag="ACG8ZM_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493417.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
BASE COUNT         2346 a         1095 c         1042 g         2426 t
ORIGIN      
        1 cattcattac cttcatcatc ttcaggtagc aagccgaatc caattggatt atcagtcaat
       61 ttttgatagt ataaatcatc ctcgtctaat gctaaaacat ctaatccttt acaatttccg
      121 cctcttctaa acgtcacaaa tcctttgtaa tcccaacgtt ggaaagaaac cttaatagta
      181 tgtgtgccca atcgtatatt cggatcataa tttttcattt gtcattcctc cattacgtaa
      241 atttcatttt ctctatgttt tatatcaatt ttttcacttt attcaggact cgttcatatt
      301 ttatcgtttt tcgtttactt aaaaatacat aaaaaatatc gactcgttta tggcgtccga
      361 aacgagaaac cctagtagaa atcggtaaat gctcgattga tccaatgacc aacgtcctcg
      421 caagtatcta catgtactgc agcacacgac acaatttctt gtgcgtcctc cccattacga
      481 gaagacctac tccttattcc atgagtttgc gtcaaagcct agatccctta gtagctgctc
      541 atgattaatc taggcgctat tctaacttgt ctaacttcta aattccctct tgtttactct
      601 gtgcatatta tttacacaaa ttcatttcac cactatccaa acaagggcat ttactcgatt
      661 gaccaatttt ttacgttacc aatcgtaaaa acctaccccg tcaccccccg aaaggaattg
      721 ttgatagatc aagtctactc cttatttttc tagtaattct aagttctttt agaagtctcg
      781 acaatatttt caagcaagcg aaaaacgata aaatacaagt tatgagaatg atcaattcag
      841 ctgattaaac gccccatccc ttttgcggta caacgcaaga ctccattttt tcttcgacca
      901 atcagtacac cgttttggtc ataaacatgt ttgattgttg gatatagttc ttttaaaggg
      961 ataggatctt ttaaaattat ttgcttttgt ttaccagcgt aataaatcat ttcctctgag
     1021 actttgtctt tctggattat atatttctta cactgcacaa cggttttact ttggccagtt
     1081 gatagtttct tcgtgatttt ctctgtagcg gtacaccgtt taccgtagtg acaaatatct
     1141 tcgagactca aaattcctgt atatgacata aaataatcca cccacttact ttaaatattt
     1201 ttaataagtt tttcttgtta tttttcaaaa atagagaatt gattttcgtt catgacaaac
     1261 aaaaaagagg gaacgcatcc aaacatgcgt tccctctaat ttcagcgttc gtcatacggc
     1321 aaagagccac acggacattt ttataacatt atgcttaggc atagattcat cgtaatatca
     1381 cgtattttta accttaacac agccaaaaaa agtggtatca cttttgaata tctgttatac
     1441 taaatccgtt gcgggtaata ccgtaacact gaaaggtaaa cgcattaagg gattaaaggt
     1501 tgtcttaatg ccacctgtgg acgtcgacga aggttaccag ccttccgagt ctcacagggc
     1561 ctttcttttt tttgtatttt caattgtcca aatttatcgt ccaaaagtgc agaacgaaaa
     1621 ttaattcatc acaagcgtag cagacaatta aatttaaaac aactaaattt atacaaaaaa
     1681 atgtaagcga ataatattcg tattgcctga aaagaagtaa tatcaaggtt tttagctttt
     1741 ttaaaaaatg aaattacctc tcttcgcgta ttattataac taaaaaacag caacatgcaa
     1801 acgtttgttc tcttttttgt gttggaaatt aattattaac tattaacact tatgacgtaa
     1861 aaagctacgt ttgtgatttt tatcataata ttttaactca taaattctat taaaatagct
     1921 atttctttgt aacatttgta atataattag aataataaaa aatttttaat tttaatgcta
     1981 ctccatgaag caacaatcaa tttgtcagtg aaatttcact ctaaccagtc tttcgtaata
     2041 gtttttggga agctttcaga attcaaacgt ttttcacttg atactttagc agctattggt
     2101 ttttcgataa accaattttt tacatagaca aaaatcaggt tctggatatt ttcaactttt
     2161 tcggttttta atttttgata ggcttttaac agtgtcgtgt ataattcttg ttctgcattg
     2221 acataataac tatcgagttc ttcaaaaatt attgaaatac cactttctgt ttcggctttt
     2281 ttctttgcat tatgaatagt ctggacagtt ttttgagctt cactgtaact tgaagaaaat
     2341 gtttttatta aatttaatac tcgttcaggg ataaacgtag caatgctgtc gtttgtcata
     2401 attgtctcaa aattgtctaa caacaattga tcttgtaggc ggtctttttc agtgtctata
     2461 ttgtgtctat tagtgtctaa tatattatta ttaagttctg gtatgatttt cataccctct
     2521 gatttatcaa cgttttggca cggtatgatt ttcataccct cttgagcccc aagggattca
     2581 gcgtctgatt tttcgtcgag ggtgggtatg attttcatac cctctgattt atcaacgttt
     2641 tccctattct ctctatcaaa tttttcgagt aagtagatat cattttctga gacttttaat
     2701 tctgctagat atagtctatt aggattattt ttccctaact gtttgttaaa tcccatctgc
     2761 ttttgtaata ataaaccaaa ggattccaac tcttttttga ttgctatcgc tttttgtttt
     2821 tggcagttta atacgctaca tagttcattt gtcgtataag taaaataaac attaccttct
     2881 tcatcgattt gccgtttgct gattgcgatc tctaatcttg cttttaaaag catataggca
     2941 atcttagttg aatctttcat ttttttgtat tcgtcagaaa caataaagac ttttggaaat
     3001 tgataaaata attcgttgta aactctatcc gccttaaaaa aattaaaatc agtcattgta
     3061 gatcctctac tttcacgttg ttttatttat gtaaaattac ctactctttt cgaaaatagc
     3121 aaagataaat aaaaaatgca aacgcattaa aactgttttt taattaaata ctacctaaaa
     3181 tcagaaataa aatgaattat gtactacctt taaagtcatt aagaactact ttaaataaat
     3241 attatttata attaaatact accagaagaa cgtatgaata aaatttagtt ttaaaattaa
     3301 agataattaa gtactaccag tgtcaaaaca aaaaataaat taagaactac cttatgcata
     3361 taaaacatac taccattact agtaatatat actaccttat ttcagtagta tatattatat
     3421 tcttcttata agacaaggct ttaggctaga aaacaataga ttatatacta ctaaaaaata
     3481 caataagtac taccaatgtc agatatatat aataattatg tactaccatt aaaattaact
     3541 tatttaataa gatactactg tgttaatctg aaaaaaatta ctttaaagta gttacatact
     3601 acctgttaaa ttagatacta ccattaatac ttaaattaga tactacctat atttatatta
     3661 agtactaccg ttctaggaat aagataataa aaaaaaacta ccaaattgca attaaatact
     3721 acttaaaatg taaaaaataa gaactaccca aattaggtag ttcttaataa gagaatatca
     3781 gataaatttg tacaagtcat aaaaaaatta atcttaaatt tttaaatata tatacctgtt
     3841 aattataaac tactttattt aggtagtaca taattaacag ataaaactac atgcttgtag
     3901 tttgtctagc taatgagttg catatgattt atataaaggt gtgcagcata acttagttga
     3961 agttaagaga atagaataaa tattttttgt tttgctaata attaaacact acctaaatat
     4021 ggtagtgttt aattatataa tattcatttt gttacaaata atcgtattat aaaagcataa
     4081 caattgggat aactattttt tagattattt aagtactact taaattaggt agtacttaaa
     4141 ttttggttta aggtagtagg taattaagaa ataatatata tcttctttta taatagaagg
     4201 ggagaggcct agttaaaaac agcgacaatt aataacaatg tatttaatgc ttcacaaaat
     4261 ccatttggtg gacaatgcat tgttattagc gatgatctac cgttctaagt agttttttct
     4321 attggttgca ttcgtgacta attatggtat aattatgagt aacaaagagg tatttcatta
     4381 tatcatcaaa ccccatcagc gtgtaggctg atggggtttt ttttacgttt ctatattata
     4441 gaattaactg gtaggcttac tgtctttttt ggcaattttg caatcaagcc acttgcagat
     4501 gtagtgtgca gttacctctg caaatggctt gattgcaaaa acaaaatcaa aaaggtattt
     4561 cattatatct cctcctcccc ttgccagatt aggccggggt agcagacagc aaaactatta
     4621 taacataact gaaataccta aatgacatat acagatatga taatttatgt attgattttt
     4681 ctaacaaaag cacatatttt aatatatact acctatttta ggtagtatat atttttagat
     4741 acaatatcga tcagttttga ggaaatgaaa ttctagatat tttttgctac atagagttac
     4801 tttcaatatt ttttaacgta attaggcaga agtcactcat cctctatagg tttgtgatga
     4861 aattgctatt gatccattta agtaagagca aaaaactgga ggtttatcag gtaattggtc
     4921 taatggattt aagtatgcgg tcaccgtgta tagagttgct tatgagattc aagaaaataa
     4981 agttattcca gtattgctat atggaacaca taacaatttt tatgaattag ccgagaacac
     5041 tcgcaacttt attcgtgtag atgaatcggc ttcggttgag ttataacttt agctatatca
     5101 atcaaccgac cgacatatgt taaatctata tataataaaa atacctactt aaatggatgt
     5161 taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta tatgttttca
     5221 gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct taagaaacac
     5281 cgttggcaat tgtcgttata acatgttata atttatttat caaaaactaa aagaagtgtg
     5341 ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga gtgatccttc
     5401 caagctctct tggattaaaa agcggagaca ctatccaagc caaacaagaa ggcaacttat
     5461 tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt gaagaaagtt
     5521 tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa gctttcggaa
     5581 aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa agtttccaga
     5641 ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct ccagccagca
     5701 taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat tttccaacta
     5761 tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg atcgtgatcg
     5821 gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata cttgtaggac
     5881 gtattttcca atctaaacaa atgaaactag gcttttaaca ataattaggc aaaagtcgct
     5941 catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt cggttactcg
     6001 aagattgata tataagactt agtacctttt tattttgctc catgttatgg ccgaaggtct
     6061 aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg gggatcgctt
     6121 ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc tctattccca
     6181 aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc aaaattgacg
     6241 taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt taaaagagga
     6301 agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa aatatacatc
     6361 cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa aggaatggga
     6421 taaaatatag gtacaataac tatttgatgc taaatataat taatggagga gagatagtga
     6481 acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt actactttca
     6541 aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa ggatacaagt
     6601 ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt ctttctgaga
     6661 gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt gatatatata
     6721 cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc agtgaatatc
     6781 tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta ctaccatatt
     6841 tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca ggtaatcaag
     6901 gaaatatga
//
LOCUS       DAZKOR010000111         6869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000111 DAZKOR010000000
VERSION     DAZKOR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.114"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACG8ZM_002494"
     CDS             <1..227
                     /locus_tag="ACG8ZM_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493418.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..1620
                     /locus_tag="ACG8ZM_002495"
                     /pseudo
     CDS             227..1620
                     /locus_tag="ACG8ZM_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1635..1841
                     /locus_tag="ACG8ZM_002496"
     CDS             1635..1841
                     /locus_tag="ACG8ZM_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493419.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="ACG8ZM_002497"
     CDS             1862..2458
                     /locus_tag="ACG8ZM_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HFQ0493420.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            3410..4009
                     /locus_tag="ACG8ZM_002498"
     CDS             3410..4009
                     /locus_tag="ACG8ZM_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0493421.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            4058..4774
                     /locus_tag="ACG8ZM_002499"
     CDS             4058..4774
                     /locus_tag="ACG8ZM_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFQ0493422.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            4788..5567
                     /locus_tag="ACG8ZM_002500"
     CDS             4788..5567
                     /locus_tag="ACG8ZM_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFQ0493423.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            5650..6006
                     /locus_tag="ACG8ZM_002501"
     CDS             5650..6006
                     /locus_tag="ACG8ZM_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0493424.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            5996..6262
                     /locus_tag="ACG8ZM_002502"
     CDS             5996..6262
                     /locus_tag="ACG8ZM_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0493425.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            6421..6699
                     /locus_tag="ACG8ZM_002503"
     CDS             6421..6699
                     /locus_tag="ACG8ZM_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493426.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(6696..>6869)
                     /locus_tag="ACG8ZM_002504"
     CDS             complement(6696..>6869)
                     /locus_tag="ACG8ZM_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0493427.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT         2527 a          957 c         1333 g         2052 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaag ataaattatt tttttggtat tcatataagg ttaattatgt
     2941 attatcacaa aatgattgtt gtcgaataac taaaagtgtc aaaacaatga tgaaatcatt
     3001 tagttcttat aaattagaca agagaaaata aatattccat atctacttta ttggatccat
     3061 aacatgacta aattctgaat actttttaaa gcttatcatt tggtaaaact gaatataagt
     3121 taattatata gactgaaaat tttgaatttt cagaatagaa taacttaaaa gtactgacaa
     3181 attttttaaa ggggcaatat taatgttctg agataatcaa atcttaccga aaatatcaaa
     3241 agtccaatgt gacacaattg gattttgtca cattcgttaa aatcgctcca tccttagagg
     3301 ctctaatagg aaatcacttt tcttttagcg ccttttttct atagcttgtt taaatgtgac
     3361 ttattatctg ttataatcgt attaagtaac atttgagagg tgtctaaaaa tgaaaagcgt
     3421 ggaaccgatt cgcgacaaaa aaaagatcga tgccatgaag gccattttgg cttccggaaa
     3481 atatgggcaa cgaaatttgg tgcttttttc gatcgggatc aatacagcct atcgaatctc
     3541 cgatttaaga cagttaaaat taagggatgt gctagaaatt tcccgtgggc gggtgatcgt
     3601 caaagaacgt ctggccatga aggaacaaaa aacggccaaa cacaattcgg tcttcatttc
     3661 gaacaaattg cgaaaagtga ttcttgatta tgttcaatcc gaatttcctg agcagttgca
     3721 agcacaagac ttcagcaagt atttgtttcc aagtcgaaaa ggagcagata cgcctttgac
     3781 ccgacaaagc ctttggcgga tcattcatga agcaggaact gcagttgggt taaaagaaat
     3841 tggtccccat tcgatgcgta agacctttgg ctatttttta tacaagcaag gaaccaaaac
     3901 agagatcatt cagtcgctgc taaaccattc ttcacaacgg gagaccttac gctatattgg
     3961 aatcacccag gaagacaaag atactgcagt aaagagtttg gatttatgac agaataatgc
     4021 cctatataat agaaggaaaa attcaagagg tgagaaaatg aacgaagaaa agcagcctaa
     4081 ctttcctgat aaatatcact tatctcgcaa agagtctgtg tatttattga agaaaaacat
     4141 tgtagaactg gtgtataatg cggggaaatt tgaagggtta aatacaacgt tgttacaaac
     4201 agaagggatt attaagtata atcgggccaa caacgtggct gtagatgatg tcctgaccgt
     4261 cgttaactta aaaagaggct ttgaaatgct tttaaatgac gtacaggagc ccctgttaga
     4321 gacaagtaag cggattaatc ggatcgttgc agcagaggat gcgctttttc ccggtgaaat
     4381 acgaaccggt ggggtagaat tatcgacgat tcaaggaaga tatgtgccac caatgttaac
     4441 tgaagacgag gtaaacaatc aatatggtga gataatgaat caagagatat cagatacgga
     4501 aaaagccctg cgactgtttc tattcatttc gaaaaatcaa atattctgga atggtaatgt
     4561 gggtacagcc ttgttgattg cgaataagat tatgtttagc aacggtttag gactattatc
     4621 aatacccgaa aatgtattta tgaaatttaa cgaactttta tcaaagtatt gcaacagtaa
     4681 tctattagtc aatgagtcaa atattatatc atttttatat gaacagtgta tctttggtat
     4741 taattatttg aaaataatcc aacatacaat ttaagaggta taagtaaatg atgactatag
     4801 acgaagcaaa acaattagca acttattttt cagttggttc agcgggatat attttagact
     4861 ttactaatgt aacgtggaac atatttacaa agaaaatcgt agggattgaa atacaaaatc
     4921 attatggggg ctcaaaggga gtatcctttc aaaagtttct gcttgacgat caatttgatg
     4981 attcgttaaa gaatcggtta atccaatctt taaaaggtat tatggatcga gataaaaaat
     5041 atgaatctac tgaaatgaat cccatagttc agttgctttt tgaggaagta tttaaagaag
     5101 atacttcaac tgcattaaga aaaatgaaat tttcaagcga gattgattta ctttatattc
     5161 ggcagctacc cattaggatt caaacagata ttaaaaacaa ggattttgac tctgttttga
     5221 caaaagcaaa tactttaata gatgaagttc taaaatttat tattgaaaat accgaaggac
     5281 cgagtgttaa tattaattta tttaagtcaa aagaattaag aaaagaagta tttggccgcc
     5341 tcaatattaa aactgaaaaa gatatggata atagaatcaa agcccttgtt ggagcactca
     5401 atacattatc agataaaata ctcgaaatga gaaatagtca aggagatgct catgcacatg
     5461 gctcaaatag aatagtaatt aaggaagaag aagcaattct tgttgcaaat tcttctatgg
     5521 ttttgtgcga gtatgtgttt actaagtata aaggattaaa tagataataa aatgcatatt
     5581 ttatagtata caattcgaat ttttatgtta tatactataa tataaaatca atatatgata
     5641 taattgggta tggaaaaaat atcttttgaa acatttaaaa gacccaatgg tcatgatgaa
     5701 tttttagaat ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata
     5761 gaagaaacag aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta
     5821 gacactaatt tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agccatctat
     5881 ttccatgtgg acaatggaag gtatgtcatt actcacggat ttactaaaaa aacggagaaa
     5941 acaccaataa ctgaactcaa gcatgcaaaa gaacttagag aggagtggtt cacaaatgat
     6001 gattaaaaaa ttgattgaag aaaagaaaaa agataaagat tttgcagaag catatgaccg
     6061 tgaaggaaga aaactcgaaa ctgcggttgc tctttatcat gctcgggagg aagcagggct
     6121 aactcaagcg gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga
     6181 gagaggggat aacgtatctt tcgataaact tgatgatatc gccagggcat taggtaaaaa
     6241 gctggtagta gaatttgctt aaaagcgagt tgaaaatata tctataatag gctcctaact
     6301 aatcaattag tttagtgggc ctttttttac tagatagata aaggttgtac tatagtgtaa
     6361 aagacaataa attttatact aaaattactt taaagagaac tagagagaaa ggtacaataa
     6421 atgaaaacca taaatcttat aaaagtattt atagcatcac caggtgatat agcagcaaaa
     6481 aggaatgaag tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa
     6541 gtgattttga tgcctatacg atgggaaact aatgcgattc ctacttatgt aaataatagc
     6601 tctgggcaaa gtgttattaa taaacaaatt gtagaaaata gggttctgct gcaaagtttt
     6661 aaaaataaaa tcagtttttg tattatttct tcattttaag ctggaattcc caataatatc
     6721 ctgatttcag tgcagactga aaagccgaag agagtgcctt cttttcggct tttcttgtag
     6781 attcctcgaa tggtttccat ccctttaatt gtggtggaag ctgtacgtag actttgatac
     6841 aatttatgtc gacgttttat cgatcgatg
//
LOCUS       DAZKOR010000112         6861 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000112 DAZKOR010000000
VERSION     DAZKOR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.115"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8ZM_002505"
     CDS             complement(<1..182)
                     /locus_tag="ACG8ZM_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0493428.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8ZM_002506"
     CDS             517..951
                     /locus_tag="ACG8ZM_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0493429.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8ZM_002507"
     CDS             974..1294
                     /locus_tag="ACG8ZM_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493430.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8ZM_002508"
     CDS             1435..1626
                     /locus_tag="ACG8ZM_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493431.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8ZM_002509"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8ZM_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0493432.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8ZM_002510"
     CDS             2112..2375
                     /locus_tag="ACG8ZM_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493433.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8ZM_002511"
     CDS             2399..2587
                     /locus_tag="ACG8ZM_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493434.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8ZM_002512"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8ZM_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493435.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8ZM_002513"
     CDS             3070..3441
                     /locus_tag="ACG8ZM_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0493436.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG8ZM_002514"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG8ZM_002514"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG8ZM_002515"
     CDS             complement(3709..4137)
                     /locus_tag="ACG8ZM_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493437.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG8ZM_002516"
     CDS             complement(4197..4955)
                     /locus_tag="ACG8ZM_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0493438.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACG8ZM_002517"
     CDS             5250..5447
                     /locus_tag="ACG8ZM_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493439.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACG8ZM_002518"
     CDS             5553..6035
                     /locus_tag="ACG8ZM_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493440.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACG8ZM_002519"
     CDS             complement(6093..6689)
                     /locus_tag="ACG8ZM_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0493441.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2327 a         1057 c         1348 g         2129 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct t
//
LOCUS       DAZKOR010000113         6826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000113 DAZKOR010000000
VERSION     DAZKOR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.116"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..950
                     /locus_tag="ACG8ZM_002520"
     CDS             60..950
                     /locus_tag="ACG8ZM_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0493442.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1023..1847)
                     /gene="nadE"
                     /locus_tag="ACG8ZM_002521"
     CDS             complement(1023..1847)
                     /gene="nadE"
                     /locus_tag="ACG8ZM_002521"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0493443.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1859..3331)
                     /locus_tag="ACG8ZM_002522"
     CDS             complement(1859..3331)
                     /locus_tag="ACG8ZM_002522"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0493444.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3968..4330)
                     /locus_tag="ACG8ZM_002523"
     CDS             complement(3968..4330)
                     /locus_tag="ACG8ZM_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0493445.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4553..5305
                     /locus_tag="ACG8ZM_002524"
     CDS             4553..5305
                     /locus_tag="ACG8ZM_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0493446.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5445..6368)
                     /locus_tag="ACG8ZM_002525"
     CDS             complement(5445..6368)
                     /locus_tag="ACG8ZM_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0493447.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6383..6655)
                     /locus_tag="ACG8ZM_002526"
     CDS             complement(6383..6655)
                     /locus_tag="ACG8ZM_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493448.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2092 a         1354 c         1162 g         2218 t
ORIGIN      
        1 ataactttta ttaacaaata tgttaatata taagaaaaac cttttaagag gtgatatata
       61 tgcaactaca aataaacgaa gaatccttac cggtatatga agcattagct agtaaaacaa
      121 ggatcaagat catccagcta ctttccaaaa aaaagatgaa tgtcaaagat ttggcaaaag
      181 aacttggagt gagcagtgct atcacgacga tgcacgtgaa aaagctagag gaagcaaaca
      241 tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc gataaaatcg
      301 atatcagttt tccagaaaaa atattcaatg cttttgatac gaaagaaaca tcgataccta
      361 ttggtcacta tacaaattat gcaattgaac caacatgcgg tttagctacg atccatgatt
      421 ttatcgggaa agtggatgaa ccacgctatt ttatggatcc tcgaaggatg gatgcccgca
      481 ttctctggtt taccagcgga tttgtcgagt atcaggcacc taatttttta aatacagagg
      541 atacattgga gatgttggaa gtttctgtgg aaatttcttc tgaatttcct ttttcaaatg
      601 acaattggcc ttcagatatc actttcagcc taaatggtgt ggagctagga acatggacga
      661 gccctggaga ttttgctgat atccgcggta aatatacgcc tgattggtat ccagataatc
      721 ttaatcaata cggcttgctg aagacgatcc gaattaccaa acacttgacg aatatgaatg
      781 gagaaccctt gtcaaacatc acgatcaacg acatccctaa agagcaagac acttggcatt
      841 tgcgaatcga agtgaaagac gatgccaaac atgtgggggg ctgtacttta tttggtaaag
      901 gattcggtaa ttacgatcag gatatcaaat taaaagttta ctacagctga tatttttctt
      961 ttctatttta taaaacaaaa acagccaatc cctatgaagg gacggctgtt tttgctttat
     1021 tgttatttcc agaaatcatc caaaacggaa attggcaaat gtcgtttatg ttgtgtcttg
     1081 ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc ttctagataa
     1141 tcatcaattt cattatatgt gacacctagt gcgacttcat ctgcaatcat tggtttccca
     1201 tcttcaagat ctgcagtcgg aatttttgtg tataattttt ccggtgcatt caatgcttga
     1261 agcaattgtt tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc ggctccacca
     1321 tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag catgatctgt ccctaatacg
     1381 gctcctgctc tttctccagc tacggcatat tgcgtaatca tacgttgacg tgctttgata
     1441 tttcctttgt taaaatcgct gattcccaca cctgcttcgc ttaatacttg tacttgtgca
     1501 tcaacagctg gtttgatatt tactcgcaga ctaacatcgg gctggataaa gtcaagagca
     1561 gcttttgcat cttcttcatc tgcttgttca ccataaggaa gacgaacagc gataaattga
     1621 tagttttcat cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc caaacggcct
     1681 gctaaagtag aatcttggcc gccgctgata cctagtacaa aagttttaag aaacggatat
     1741 tttttcaaat atgctttcat aaagtcaata cttttacgta tttcttcttt tggatcaatt
     1801 gttggtttga cacctaattc attgataatt tctgtctgca ggctcattta ttctcctcct
     1861 atgcttctgt atgaagttct gctacgcttt ttttcattcg ttccatgata gccattttat
     1921 ggttccagca ttcagtagac agatcgactg ggtatttttg cggattcaag tctcgtttat
     1981 actcttccca tagtgaatct agattttctt ttgtatattc tttgatttcg tttagtgcag
     2041 gtaattcgta aactcttttt ccttcgataa agatatcttg taaaactggg cgagctgtaa
     2101 agtctcgaac agttttattg ataaacgtat gcactggatg gaacatgaag atagcttctt
     2161 cttctcgagg gtcttcatcc cataatgtca cgtagtctcc ttcagatttc ccatcaaaat
     2221 ttcgagtgat gcgccacact tgttttttcc ctggagtagt tactttttct gcattgcttg
     2281 ataacttgat cgtatccatc atttttcctt catcatcttc gatcgatact agcttgaata
     2341 ctgcaccaag ggctggctga tcataggctg tgatcaattt tgtcccaaca ccccatacat
     2401 caattttagc tttttgcatc tttaaattca aaatcgttgc ttcatccaag tcattcgaag
     2461 catagatttt tgcttccgta aatccggctg catctaattg ttcccgaaca cgcttagata
     2521 tataggccat atctccactg tcgatgcgta cgccttggaa gttgattttg tcacctaatt
     2581 ctttcgccac gcgaatcgca cttggcacac ctgatttcaa cgtatcataa gtgtcgacta
     2641 agaacacgca gtctttatgg gttttagcgt aggccataaa tgcttcataa tcatttccat
     2701 aagattggac taacgaatga gcatgggtcc cgctagctgg tatcccaaat atcttcccag
     2761 cacgtacatt gcttgttgca tctgctccgc cgatatatgc cgctctagtt ccccagactg
     2821 ctgcatccag ttcttgtgcc cgtctagttc caaattccaa tagtggatct tctccgatga
     2881 cagatttgat acgagctgct tttgtcgcga tcaacgtttg gaaattgacc atattcaaca
     2941 gtgctgtttc tactaattgg cagtgcgcta aaggcccttc cacttggatt aatggttcat
     3001 ttgcaaaaac tagttctcct tcacgagcag aacgaactgt tgctttaaat tcaaaatttt
     3061 gtaaatactc caaaaaacct tcaggataga cttctaatcc acgcaagtaa gcaatatcgg
     3121 aatcattgaa cgtcagattc tctaaataat tgactaaccg ttccaatcct gcaaaaaccg
     3181 cgtatccgtg gttaaaaggc atttctcgaa agtaacattc aaaaacagca tgtaaatcag
     3241 ctcgacctaa ttcccagtat gtctgcatca tattgatttg atatagatcc gtatgcagcg
     3301 tcaaactgtc gtctgggtaa gtattattca tcttgtatta ctcctcttgg caagttttca
     3361 tttcctaccc attataacaa aaaaaattaa taggaaaaga aatcttccat tttttctgaa
     3421 aaagcacatg ttttacggat atttcttgct aaaaatgaac tccatctgct aaaactatcc
     3481 tttcttattg gtatttaacc taaacgagaa agaatactta ttttattcaa tgtatccgag
     3541 acacgaagaa attagcgttt tctatttttc ttacttgata aaataagtag attttcctac
     3601 tacaatcgta gccgttaaga taccgatgat tgaacaaata taaccaaatg tctctacaac
     3661 ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt tccttttttg cttcttatcc
     3721 ccaaagaaac ggatataact tattatgtat tgaatttttt tggaaaggcg aattatgaca
     3781 agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt aaaaagatac
     3841 aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat ggcgagacat caatcagtct
     3901 cgccttgttt tttattgtat acattagata tggagcaggt taccataaag atgattatta
     3961 gtcgttatta aatagaatcc ttcgtatttt tttgttctat cactacaata tctttatttg
     4021 gacgtttgaa ttgtattccg gcacgttcta gctcctgttt cactcctaca tggactttga
     4081 catcttctcg tgtcattttt aaaacgtgat gaatcgcata gtcgatttct tgccttgtga
     4141 tttttttctt cgtattgatc atcacgactt gctttttatc atatgtgtct accaatagca
     4201 gttgtgttcg atctaacgaa aataatttgt aataattaat agccgtattc ccaaaaaaga
     4261 attccactac tttaggataa aatgtcttaa tatttaaatt actattcaca ggtgtttcga
     4321 ttagcttcat ctcattcctc ttttctatat agtcgattta gatccaatct taagttatgt
     4381 taccatcttt cctagcatta aggaatcggt tacaattgaa cctttaaaga caaaaggatt
     4441 taccttttat cacttaatag tataacataa tttcgtgaga aaaaaataca attttttctt
     4501 gaccatctcg ttacgtaaag gattatgctt acatttgtca ggaggaaaaa taatggagta
     4561 cacaataaag aaaatgtcag agatatcagg agtcagtccg agaaccttgc gcttttatga
     4621 tgagattgga ctgctaaaac cagctaggat caattcttca ggttatcgga tttatggcaa
     4681 aaaagaagtc gatcgtctgc aacacatctt attttatcga acaatggcat tcaagttgga
     4741 tgatatccaa gaggtcttag acaatccatc ttttgaccac cagaaagctt taatcaagca
     4801 tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc ctcttgacaa ctgtccaaca
     4861 gacacttgat atgtacgaag gaggaagaaa aatgagtgac aaagaaaaat ttgaagggtt
     4921 taaacaacaa aagttgaaag aaaatgaaga atcctatgga caagagattc gacaaaaata
     4981 cggaaaaaaa aaggttacag catccaatga aaaatggatg aatatggata aagaaacgta
     5041 tgaagaaatg caaactgttg aaaagcagat gttatttgat ctcactcttt acttaaaaga
     5101 accagaagat gaaagcttag cagaccgtat cttccaagct cataagaagt ggttctctta
     5161 cagctggcct gaatatcagc caaaagcgca taaaggacta ggaatgatgt acgaagccga
     5221 tgaacggttc actgcatatt atgacgagcg aagtggtcat ggtgcagcaa aagctttaaa
     5281 tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc aacaaaaaag tacacgtaaa
     5341 atgacctaat aacaaaacag cagacaccca agttagattt ttatgctaac ttttggtgtc
     5401 tgctccattc tttcacaagt tttttcatcc tactttaaat atattcatcg ttgaataaac
     5461 gtataaggta ttttcacttt atcctttttt tctgtaagaa acaattgaaa tgccatttct
     5521 ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa gaagctcgct gatcagcaaa
     5581 ttatcgcgtc caatcaccac tggtgtctta tcttgcagat agctttgcaa gatgcctgct
     5641 gcaaccatat ccgaatttgt aaagacacca tcaactccaa gctctttaaa aaagaagcct
     5701 gcttgttttc ctttctctgc atctatacaa tcccaaaaaa caagtgattc atcaaaagat
     5761 ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt tataacttag cctgccactt
     5821 cgccccaacg tgatgcccaa ttgctttata ccttgctgct tcaaataagt aagaccttct
     5881 gtaattgatt gttctcgatc aataaagaca cagcttgcct gttttccctt gatttcttca
     5941 caaaaaacta gaggtccata tttttgatat gccaataaat catctagtgt attggcacga
     6001 gacgtaatga tcaatccatc gaaagcttta gctgcaaatt cttcaagata ccttcgctct
     6061 ttttctttat gatagcccgt cggtaaaagt gtcactttgt aatcataatc aaacgctgct
     6121 tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat tggtataagg cagaatcact
     6181 ccaatgcttt tcgtttttcc ataacttaac tgtctggcat ttgcattagg gatataatcg
     6241 acttctttca tgaccgcaag tatttctttc cttttttcct catctacata tggatgtcca
     6301 ttgattacac gtgaaatagt agaaactgaa taacctgtga gctttgcgat atctcgaatg
     6361 tttgccactg ctgcttcgcc acctatacaa aaagattttc ttgtaaatag tctaccacag
     6421 ctggaccgca ttcaaaaaac tgatacattt ttccttcatg cgaaaaactg atccgctgcc
     6481 cgccccaata tatatctttt gtcaaatttt cgatttcatt cattggtatt tcttttacta
     6541 tattcctatt caacccttga aaactgtaca acgttaatac ggatcccttg aactcgacaa
     6601 acgcataaaa ataattgttc gttgtgccat ctgttatctc tacataagta ttcattgcaa
     6661 gccctccttt tgatactttg agtatagtgt atgaaacgcg tctcatagca aggagtttgt
     6721 tttgccaaag aaaagtttta ttcctctttc attattacca aagtctctta ttttaaattg
     6781 aatacattat aatgatactg taaatatatc tttattatta gtgaat
//
LOCUS       DAZKOR010000114         6643 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000114 DAZKOR010000000
VERSION     DAZKOR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.117"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG8ZM_002527"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG8ZM_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8ZM_002528"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG8ZM_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0493449.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG8ZM_002529"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG8ZM_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG8ZM_002530"
     CDS             complement(2143..2373)
                     /locus_tag="ACG8ZM_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0493450.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG8ZM_002531"
     CDS             complement(2603..3421)
                     /locus_tag="ACG8ZM_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0493451.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG8ZM_002532"
     CDS             3582..4271
                     /locus_tag="ACG8ZM_002532"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0493452.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG8ZM_002533"
     CDS             4285..5787
                     /locus_tag="ACG8ZM_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0493453.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG8ZM_002534"
     CDS             5800..6276
                     /locus_tag="ACG8ZM_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0493454.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG8ZM_002535"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG8ZM_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAZKOR010000115         6601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000115 DAZKOR010000000
VERSION     DAZKOR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.118"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(252..1463)
                     /locus_tag="ACG8ZM_002536"
     CDS             complement(252..1463)
                     /locus_tag="ACG8ZM_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0493455.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
     gene            complement(1581..2216)
                     /locus_tag="ACG8ZM_002537"
     CDS             complement(1581..2216)
                     /locus_tag="ACG8ZM_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFQ0493456.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2299..2721)
                     /locus_tag="ACG8ZM_002538"
     CDS             complement(2299..2721)
                     /locus_tag="ACG8ZM_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0493457.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(2739..3059)
                     /locus_tag="ACG8ZM_002539"
     CDS             complement(2739..3059)
                     /locus_tag="ACG8ZM_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0493458.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3352..3543
                     /locus_tag="ACG8ZM_002540"
     CDS             3352..3543
                     /locus_tag="ACG8ZM_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493459.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            3557..3808
                     /locus_tag="ACG8ZM_002541"
     CDS             3557..3808
                     /locus_tag="ACG8ZM_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFQ0493460.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            3784..3963
                     /locus_tag="ACG8ZM_002542"
     CDS             3784..3963
                     /locus_tag="ACG8ZM_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493461.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGNRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(4006..4476)
                     /locus_tag="ACG8ZM_002543"
     CDS             complement(4006..4476)
                     /locus_tag="ACG8ZM_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493462.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            4563..5261
                     /locus_tag="ACG8ZM_002544"
     CDS             4563..5261
                     /locus_tag="ACG8ZM_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0493463.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            5439..5780
                     /locus_tag="ACG8ZM_002545"
     CDS             5439..5780
                     /locus_tag="ACG8ZM_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493464.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            5773..6444
                     /locus_tag="ACG8ZM_002546"
     CDS             5773..6444
                     /locus_tag="ACG8ZM_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0493465.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
BASE COUNT         2146 a         1081 c         1176 g         2198 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 tcgttattta atggtatttt aaatgaagat aagcgaattt acagcatttt taaatgctta
      181 tataccgcaa attatcccaa gtttttatat atcctacccg agtttatacc cgaaaatata
      241 attttttagt gttattttat atcttttttt aatagttttt caaacgctgt agttgctgta
      301 tctttttctt tgcgtaagac atggccataa acattcattg tagtattaaa atctcggtgt
      361 cctaatcgtt tttgaagctc ttttggagtc atattaggat cgctaagcaa ataggtagct
      421 gatgtatgtc taatgtcgtg aaaacgaatg tgacgcaaat tgtgacgttt ggtgaatcgt
      481 ttgaacttct tatacagata acttccacgt ggtaaagttc cgtcttgttt tgtatcaaat
      541 ataaaatagt gtcttaattt ctttatattt aatttccaac gaattttttt attctctttg
      601 atcaattcat ctaacatatc aaatagataa gcaggaccgg ttactatttt ttcatcatca
      661 tcatttttca aacctggcag caaccgaata tctgattttc catccacttc tgaaatccgc
      721 tgatgaaatc ttatttcttg tttgtcaaag tcaatatctt tttcttctaa agctgcaatt
      781 tctccttgtc ttgctcctgt aataaatgct agaacaatta aagttttaat ttctatgctt
      841 tcctcaaatg ctgcttttag cattgcatga atttcttctt ccgaataagg ttcttcgata
      901 ttctttttta cgcctttttc ttttggtatt tttacatttt ccacaggatt ttctttaatt
      961 gctccgtact catttttagc catcaaaaat aagttgttaa cggcgtataa catacgaagc
     1021 tttgtatttc ttgataaagg tttatcagag tttcttccag gatcttttgt attgattctt
     1081 tgagcattaa caacaatttt ctttatcatg tatggtttta tatcgattag agggataaaa
     1141 tcaaatttat caagaaatct attctcgatc attcgcttat agttatgata agactttggc
     1201 tctaaatttt tttcggcctc tgaaagccat atttttctat aaaattcacc aaaagttata
     1261 ttgaataaat ccaacgaatc atcggtcatt ccttcgaatt gctctaacca gatgttcaat
     1321 tctttatatg ccgctctttt actattcgct tttatagttt tgctttttct ttttgccgag
     1381 ccatcaggtt tgtaccctaa tacagctctt agtctatatg tttcattgtc tacttgttct
     1441 aagtatcctg ttacttctga cataattgaa tcagctctct ttctctgata caataggtac
     1501 tataaagaag cctattgtgt aggtttgttt tttcttagaa cacgctcgct ttggtcggtg
     1561 gggcgtgttt tttatactaa ttagttatga agagcaattg aaactgtctc tgttccaaag
     1621 taacctgttt gtgcttgcaa tacattattt tgtgcatttg cttgggcttc agagatatca
     1681 aaaactattt taccagtttg ttgcatatca ggatttaagc tttccataaa gaaagagtta
     1741 gtgatattac cattttcatc ttgatttgcc gacatagatg cagccgaatc agcttcgaaa
     1801 gtttttccat ctgcttttaa tttgaagaat gagctatcaa cagtcacagc cttatcacct
     1861 gcatttttta cagataagtc aactactaag aaagtgtctt ttgcgttagt aggaagtaca
     1921 gatgggccaa cttgttttac tacttcaacg gagtttactt tgtattccat tttccctaca
     1981 gcaacgtcat caccgatttt ataagtcttt tcttcttttg ttgtagtttc ttttgtagtt
     2041 tctttattag tacttgttga tgttgcagta gctgaatctt tgttttcacc gccacttaga
     2101 gcaccaccaa taccaaaaaa caaaagtact attaataccc aaaaccatac ccgtttataa
     2161 aacggttttt taaccttata cattttccca tcttgaccca taactttttt tgccatttaa
     2221 atatccctca cttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg
     2281 ttgcagcacg ggcttttttt actgtgcata agagtatttt ttcttgaaat atgactggca
     2341 aacattaaaa cattctgttc ttaacttatt attgatagag tagaattcca tgaaattttc
     2401 taatttgaac tgagattcat cagtcaattc gttctcaata aagatattta gtaaaatcat
     2461 aattgcgatt ctatcagctt cagcttcgaa ttttgaatga aaagttgtag agttatcgta
     2521 cagtactgaa tattcaaaat gtgaagcaat gaaatgaccg agctcatggg ctaaatgaaa
     2581 agcttcagaa ctgtcttcgt gtagtttttc attcaaaaat actattcttg gttttggata
     2641 ataaaaacct ggttcttcca tttccatata gattaacttt aaattatact cactcagcat
     2701 ttctttcaac tttaaataca tacaacccat cactccaact attcattttc ctctaaagct
     2761 ttagcaattg caatcgcttt acgcattgtc tccttagata tttcttttcc atcaaaagaa
     2821 aatacagtat catcttctga taaatccaca tgtttaggcg tttctttttc ttctctacct
     2881 agaagatagt ctacagagac attgaaatag tcagctacct tttgcaaatc tcttgctttg
     2941 ggttctgttg ttttccatcg atagaaaaga ttttcgctaa atcctaattc taaagcgact
     3001 tctttcatgc ttttatctct ttggctagct aatgatttta tcctttcaaa cagtgtcata
     3061 ataatgttct ccaatcctaa acaaataaaa gtacacaaaa tgtaaattat actgttgaca
     3121 taagtataag tttgtattat tatttgtttg taagttaatt tgatagaaaa aaagcaaact
     3181 aaaaatacac cttatagcat taagtttggc gaccgattgc ataataaagg gtttgttgta
     3241 ggcttattta actatgttta tatagtatac gattgtatac tattagtcaa tagtttatac
     3301 gttttttcta tcattttgac ttacaaatct atatgtaagg ggagtgaata gatgtcaaat
     3361 ctagataacg gccgtgctgc aatcaagaag tttatgcgag aaaacggagt aactatggaa
     3421 gatttggcta ctgcatatgg atacacacgt gttcgaatgc agcaaattat tgatggccat
     3481 tggagtggac caaaagctaa taaaactatc ttagaaatca tccgtgatta cagaattcga
     3541 taggaggaat taatcaatgg aacaactagc tttagttaac ctctcagccc tcaaagcttc
     3601 tttggcagaa tctgagattg caaatgaagt gtgggataca aagcaagctg cggaatattt
     3661 gaaaaccacg acacgcacgc taactaaaga tgccgaatct ggaaagattc cagcagcgaa
     3721 agttggcaga gagtggaggt ttagtagtat cgctttgtat cagtatctaa aaggaggaaa
     3781 aacatgggca aattcaacag agcactagta ttcagcgcac cgctaatcat ctacgcttta
     3841 gggctttggg gaaatagaca agcgttgata ggaacgattg tttacatggt ttggattttt
     3901 atggggcttg atgaagctga agctgagtac agagcgaaaa agccaaccga gggggctgac
     3961 taaaaatgaa tagaaaagag aaactagaat gtatattact attactcagt ttaattcttt
     4021 cactaatttc tctattggga agtttgtatt tttgatatca acaattttgt ggatttgggt
     4081 tctatttaga gtaaatatca tttttgtttg gtgattaagg agtttattaa aattaatttt
     4141 ccctacttca aatgcaaggt aacaagatat agcactttta ggaggaatat ttagcggtac
     4201 agcttcagag tatattctat tatcatcgac tgatcctcct ttagcaatta atttcttaaa
     4261 tggagtagct gaccatctgc ggtttaagtt agcggatgaa agttctaaat taactaacat
     4321 agctggttct gttgaatagt tagaaatgat tacttttgta taaatcatat ctgcagcgaa
     4381 gtaagaagca ttaagttcaa cttctagttg agggcgcatt ccttttattt ttatccctgt
     4441 taaaaacgta ctcaatataa aacttattat tgacatccat tgaaaaattg ttaagtgaaa
     4501 aaaattcaaa ataaacacca ccagttttta actaaattat accaaaaagg agagaagaaa
     4561 taatgcaaga attagtaatt ttgaaaaata aagaagctgt gactacgagc ttacaagtgg
     4621 cagaaagctt cgagaaaaaa catcaacatg ttttaagaga tattgatgca ctaaaaaaag
     4681 atgtgtccaa ttttggacag atgtttgtag aaggtaatga accagattca tatggcagaa
     4741 atcgacgagt tttcttcatt agtagagatg gatttttctt gctggctatg gggtttacag
     4801 gaaagaaagc tatctacttc aaacaaaaat acattgaagc attcaacgaa atggaagatg
     4861 ttattcgcaa gaatactgtt cctcaaacaa ttgaagatat gatgatctat caattagaag
     4921 aaatgaaaga tgttaaaaaa gatgtttcca tgcttaaaga tactatgcga attagcggac
     4981 aacaagagtt tgaaattaag caaaaaggaa atatgaaagt tatgggagtt ctagggggaa
     5041 aagaaagccg agcttatgaa gaaatcagca aaaaagtatt ctcaaaattt tggtctgaat
     5101 ttaaacgtac cttttcaatc ccaagatatg gcgagttacc tcgtaagaga ttcgatgatg
     5161 ctgtttcatt tattgaaatg tggttgccag aaactgcgat ccgtatggaa attgatcaac
     5221 tgaacagaca acagagactt tttggtgatg acaatgaata gagctgaagc gctaagaata
     5281 gggacggcaa ttgctaattg ctggtggaaa tactataaac caatcatcct aagccaacaa
     5341 catattgaca agcaaaaagc atggcaacaa ataaaaaagt gactccgccg gcaagcaaag
     5401 agtcacaaag aaaacacatc ataaggagat tttagcatat ggaaaaagaa ctttccactc
     5461 tagatcaata tttgattgat cctgattggg gcaagccgaa aattgaggaa acaagtggtc
     5521 gaaaaatcag acgaaatctt ttgacgaatg aagaactagc ttgggatcaa gatgatttag
     5581 gcaaccatgt aactatttgg gatcatgttt atcttatcca tctatcgaag cattcgaata
     5641 aacctgaata tatttacgtc atcgaagatg gcttgattga tgcgctagaa gagtacgaca
     5701 gagataactt gattgatatc tcttattacg gaccaggtaa gaaatacatt gctgaaatgg
     5761 aggcagaatt tgatgagtga aatcaaaggg acaacgaact ttgaaaaact ttttagtcgt
     5821 aagttaaata aaattctcaa gaaaaaagga aattttgatt atttatcttg ggctcacgcg
     5881 tgggagatta tgaaaaagaa tgatccacag gcaacggtaa ctattaatga gtacaaacac
     5941 tacagggttg tttctggaac tcatcaagac tttcttgttg aggaatataa accttttctt
     6001 atggatgaaa ctgggactta tgtatctgtc tcagtaacgg ttaaaggaca cacggaaacc
     6061 gaattatttc ctgttttaga ttatcgaaac caaccagttg ttaaaccaaa tgcaatgcaa
     6121 atcaataact cattgaagcg atgctttgtg aaagcattgg ctctacacgg actgggatta
     6181 tatgtatttc aaggggaaga tattccaaca ccacctagaa tcgatacaaa gaaattaaac
     6241 atgctagaga cgattctaga agctttcaat gagcagatgg gtaaagatat gaccaaaacc
     6301 ttaatcgaat atgttaatga gcagacagat aaattagggc tcttagctga taacgttgaa
     6361 actattgaac agttaagcta tgagcaatgt gccttgatgg agcgagcaat agcagctaag
     6421 agaaaagaat tagataagaa gtgatatgag tgtttaaacc attaatcgat tcatattcag
     6481 cggttctgaa aaagttcaaa ggaaaagaca taagcgcaac catcaatgag gaagtgaaca
     6541 ttgatcgact aaagacgatg tatgacggct acgatggtga tcgagtcatt gaaattcgtt
     6601 t
//
LOCUS       DAZKOR010000116         6551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000116 DAZKOR010000000
VERSION     DAZKOR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.119"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..780
                     /locus_tag="ACG8ZM_002547"
     CDS             43..780
                     /locus_tag="ACG8ZM_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0493466.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            794..1609
                     /locus_tag="ACG8ZM_002548"
     CDS             794..1609
                     /locus_tag="ACG8ZM_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0493467.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1622..2269
                     /locus_tag="ACG8ZM_002549"
     CDS             1622..2269
                     /locus_tag="ACG8ZM_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0493468.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2285..2941
                     /locus_tag="ACG8ZM_002550"
     CDS             2285..2941
                     /locus_tag="ACG8ZM_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0493469.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3103..3924
                     /gene="racE"
                     /locus_tag="ACG8ZM_002551"
     CDS             3103..3924
                     /gene="racE"
                     /locus_tag="ACG8ZM_002551"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0493470.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3927..5282
                     /gene="rph"
                     /locus_tag="ACG8ZM_002552"
     CDS             3927..5282
                     /gene="rph"
                     /locus_tag="ACG8ZM_002552"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0493471.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5283..5801
                     /locus_tag="ACG8ZM_002553"
     CDS             5283..5801
                     /locus_tag="ACG8ZM_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0493472.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5908..6408
                     /gene="cbpB"
                     /locus_tag="ACG8ZM_002554"
     CDS             5908..6408
                     /gene="cbpB"
                     /locus_tag="ACG8ZM_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0493473.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2158 a         1099 c         1444 g         1850 t
ORIGIN      
        1 tttaaaagaa atcttactaa ataataatgg gagtgtaatt ctatggcaat gattaaattt
       61 gatcacgtgg ataaatatta tggcaagttc cacgcattaa aaaatatcaa tctcgaattc
      121 gagaaaggag aagttgtcgt tgtcattggt ccttctggtt caggaaagag cacgatgctt
      181 cgctgtatca atggattaga gacgatctct tcaggtaagc tgctgattaa tgatactgac
      241 ttgcatgata aaaagacaaa actgacagaa gtccgaaaaa acatcggcat ggttttccaa
      301 catttcaatt tgtacccaaa taaaactgtt ctggaaaaca tcaaattagc accaatcaaa
      361 gtgttgaagc aagaccaggc aactgcagtc aaaaacgcgg agaaattttt gaaaactgtc
      421 aatatgttag ataagaaaga ttcttatcct tccatgttat ccgggggaca acagcaacgg
      481 gtggcaatcg ccagaggttt agcgatgaat cctgagatgc tgctgtttga tgaacctact
      541 tctgctttgg atccggaaat gattggtgat gtattagacg ttatgaaaaa attggctcat
      601 gacggtatgt caatgattgt cgtgacacat gaaatgggct ttgccaaaga agtggcggat
      661 cgtgttatct tcatggctga tggagaagta ttggaagaca gcagaaatgt gagagacttt
      721 ttcgaaaatc caaatgaagt ccgagcacag caatttatca gtaaagttat caatcattga
      781 taggaggaaa aagatgcgca ggttaaaaaa aatacgcctg attttccttt taggcattgc
      841 ttttcttgca ctagccggat gtaaaggaaa tgggttggca aatgaagata ttttagagcg
      901 aagcaaatct accaatgaaa tcatctgggg tgtcaagtat gatactcgat tatttggaat
      961 gatggatatt gagagcagaa ctgtacaagg atttgatgta gatattgcaa aagcaatcac
     1021 taaaaagatc cttggtgaca atggaaaaac agaatttgtt gaagtcactt ctaaaactcg
     1081 gattccatta ttgaaaaacg gaaatatcga tgcaattatt gcaacgatga cgatcacaga
     1141 tgaacgaaaa aaacaggttg acttttcaga tgtctatttc gatgctggcc aagctttgtt
     1201 ggtaaagaaa ggaagccaaa tcaaaagtgt agatgatttg aatgcttcta caacggttct
     1261 agcagtcaaa ggttctacat cggctgcgaa tatccgacag catgcaccag atgcaaaaat
     1321 tttagaatta gaaaactatg cagaagcatt tactgcttta caatctggtc agggagacgc
     1381 gatgactacg gataatgcga ttttgttagg gattgcagat gaaaatccag agtatgagtt
     1441 agttggtgga acgtttacta atgagcctta cgggattgcg atcaacaaag ggcaagaaaa
     1501 cttcttgaaa gctgttaacc aagcgctaga ggaaatgcat gcagatggca catacgataa
     1561 aatctatcaa aaatggtttc caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc
     1621 aatgagcgaa ttgatacaaa cttatggacc aatctttgtc gacgggttca agatcacatt
     1681 gttttcgagt gtcttagcac ttgtgtttag tttgattatc ggaacgttaa tggcaatctt
     1741 ccaattatca aagaataaag tagtgagcgc cttagcaaag gcatatgtag aattttttag
     1801 aaacattcca ttattaatca tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt
     1861 taatctagat ggattccaag caggtacgat tggattaacg atctatacat ctgcttttat
     1921 tgctgaaaca gtacgttcag ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact
     1981 atcttctggt tttacctatt cagaaatcat gcgttatatt gttttaccgc aagcattcaa
     2041 aatcgttatc ccgccattag gcaaccaatt tatcaacctc gtaaaaaact catctatttt
     2101 agcaatcgta gctggtttag atttgatgta tcaaggagac ttgatcgcta gtgaaacatt
     2161 caataccttc gatacgtata tcatcgttgg gttattttac ttagttatca cgctaccgtt
     2221 atcttactta atggtttatc ttgagaaaaa atgggcggtt cgttcataga aggaggggaa
     2281 atacatggat tttagcggcg ctttttcatg gatgaatatc cgttttttat tggaaggatt
     2341 aaaggttact gttgaagttt cattgttttc gatcattttt agtttcttga tcggtggtct
     2401 gacaggtgtg attcgttttg cgaatatccc tttcttttcc aaaatccttg gtgtagtgat
     2461 tgatattata cggaatcttc cattattatt gatcattttc ttcacttact ttgccttacc
     2521 tcaaatcggc attcagatga acatcttctg gtccgctgtc gcagcattaa cgatttttga
     2581 gtcagctatg ttgtcggaaa ttttccgagc tggactcaat gctgttccaa aaggacagat
     2641 ggaagcagga ttatcaacgg gactgactta tgtagaatcg atgcgaacga tcgtgttacc
     2701 gcaagcattt aaatcaatga tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga
     2761 tacttcatta gcagtaatca tttctttacc tgaactttct catcaggcac gtattattta
     2821 tggacagaac accaattatg tgcttccgat gttcatcatc atgacactta tgtacttcgt
     2881 ggtatgttac gcgctttctc tgctttccgg ttacttggaa aagagacggt acagttatta
     2941 atagatgatt cttttagcgg ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca
     3001 ttcgaaaaaa agagatttcg attgttttaa gcaagtaaga gcacttgaaa attcacgcta
     3061 aaaagtacac aatagggagg atagaattta ttgaaaaaaa ggatgatacg attgacagat
     3121 aatcgcccta tcggatttat tgattcaggt gtcggcggct tgactgtagt aaaagaagcc
     3181 ctgaaacaat taccgaatga aaatatttta tttgtaggag acacagcacg ctgcccatat
     3241 ggccctagac ccgcggaaca ggtaatccag tatacttggg aaatgacgga ttatctggtg
     3301 gagcaaggaa tcaagatgct ggtgatcgcc tgcaataccg caactgcggt ggctttagaa
     3361 gaaatcaaag ctgctctttc tattccagtc atcggtgtga tccttcccgg tactagagcg
     3421 gcagtaaaaa aaacacaaaa taaacaagtt ggcattatcg gtacgattgg tacggtaaaa
     3481 agtcaagctt atgaaaaagc actgaaagag aaagtaccag aattgactgt gacaagtctt
     3541 gcttgtccca aatttgtttc agttgtcgaa agtaatgaat accattcatc ggtggcgaaa
     3601 aaaattgtgg cagaaacatt agctccttta accactaaaa aaatcgatac attgattttg
     3661 ggatgcaccc attatccatt attacgcccc atcattcaaa atgtaatggg agaaaatgtt
     3721 caactgatcg attctggagc agaaacagta ggtgaagtat ctatgctgtt agattatttc
     3781 aatttgagca attcaccgca aaatggtcgg acattatgcc agttttatac aactggctct
     3841 gccaaacttt tcgaggaaat agctgaagac tggcttggaa tcggacactt aaatgtagaa
     3901 catatcgaat tgggaggaaa ataaggatgc gtcatgacgg gagaaaagcc caagagcttc
     3961 gcaaactgga gcttcaaaca aatgttttca aatatccaga aggatcggta gtgatccgtt
     4021 ttggtgatac aacagttatt tgttctgcaa caatagaaaa ttctgttccc ccttttttga
     4081 gagaaacagg aacaggatgg gtgacagcag aatatagtat gctgcctcga gcaacgagta
     4141 cgagaaatcg tagagaaagc agcaaaggga aactttctgg tagaacaatg gaaatccagc
     4201 gtcttattgg tcgttccttg cgtgcagtag tagacttgga aaaattagga gaacgtagta
     4261 tcatcgtaga ctgcgatgtg atccaagcgg atggcggtac tagaacagca agtatcactg
     4321 gggcatttgt ggcattgaaa ttagccattg aaaaactttt aagagaaaaa gaattgtcag
     4381 aagatccaat aaaagaacat cttgctgcag ttagcgtagg aatcttacca gatgggactt
     4441 gcgtcacaga tttggattac caagaagatt ccgcagcact tgtagacatg aatcttgtaa
     4501 tgacagaatc tggaaaattc gtggagattc aaggaactgg agaagaagca acgtttgatg
     4561 gtgaacagtt gaatgaaatg ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag
     4621 agcaaaaaca tgctttgcta acagagtttg ctcaaaatga tgagcggata gaagaaacaa
     4681 aaacaatcat cattgcgaca agaaatccag gaaaagcaga agagtttcga aatatgttca
     4741 aagaagcggg atatcatgta aaaacattac ttgactatcc tgaattgcca gatgtggaag
     4801 aaactggcag cacatttgaa gaaaatgccc gattaaaagc agaaacgatt gcacaattgc
     4861 ttgatcagcc agtcttagca gatgattcag gtttgaaagt ggatgcttta ggcggtatgc
     4921 caggaatcta ttctgcacgt tttgcagggg aacaaaaaag tgatgcgggg aataacgcca
     4981 aattattata tgaattgaca gatgtgccgg atgaaaaaag aacagcacaa ttccattgta
     5041 cgttagtatt cgcggcaccc aaaaaagaca gccttgtagt agaagcggaa tggcccggtc
     5101 gagtagcccg tataccatct ggcgaaaatg gctttgggta tgatccttta tttatcccag
     5161 aaggtaagaa acaaacagca gcagaattat cgagcgaaga aaaaaacaag atcagtcatc
     5221 gggctcaagc aatgaaaaaa ttgagtgccg agtggaaaca atggctggaa ggagagagat
     5281 agatgcggta tttagtagta agtgataatc atggagacag agatatcctc gttgacttag
     5341 tagatcgtta tcgttctgaa gtggatatct ttttccattg tggtgattcg gaattagaag
     5401 cttctgaccc attatgggat acattccaag tggttcaagg taattgtgat tatggaccag
     5461 gattcgaaca gaaaaaagtg atccaaactg gtcaggatac agtattcatg actcacggtc
     5521 atttatcgaa tgtccgattt ggtttgacac agttagcaat cgaagctcga gctgcaactg
     5581 ccgatatggt gttttttggt catacccatc aaataggctg tgagatggat cagaaaatcc
     5641 tgtttttgaa tcctggtagc atttctcagc ctagaggccc gatccaaatc ccatcatatg
     5701 ctcttatcga aagtttagat gaccaaattt ctgttcaata ctataaccgt gcccatcagg
     5761 cagttgaaga gttggccttt tctttttcga gagagcgctg aataaaaaaa ctctaatttt
     5821 taataaaaat aagctttatt ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt
     5881 gaatgaaata gaaatggagg ccatcagatg attggaccta tagtaagaga aatgctgctg
     5941 gaaaatcagg aaacgttttt gattcctgca gaaaatgtgg caaatgttat gtatcatcat
     6001 ccattgtccc atggtttact tgtgttatcg aaggtcggtt attccaaaat tcctgtatta
     6061 ggaaaagatg atcactttgt aggactagta agtttatcag atgtggtcaa taaaatgatt
     6121 gacttgcaaa caatcagcat ggacccgtta gaaggactaa cggttgctga tgtgacagaa
     6181 acagatgttc cgacaattga tgaaaattgg gaactagaag aagtactcca tttgttagtc
     6241 gattcgcctt ttttaccggt tgtcacagaa gatcggattt tcaaaggaat cattacaaga
     6301 aaagagctgc ttaaagccgt aaacttcatg gtgcatgaat tagaacgtcg taatatattg
     6361 tctccgaaaa tcgaagaatt aaaagaacaa atcgatatcg tgagctaaag aacgagaaac
     6421 taaaagataa tcaaaaaata agtagaaaaa acagcgtacc agttttgact ttatcttgaa
     6481 aaagaggctg ggacaaaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc
     6541 ttttcaaatt t
//
LOCUS       DAZKOR010000117         6376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000117 DAZKOR010000000
VERSION     DAZKOR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.120"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            252..1112
                     /gene="ylqF"
                     /locus_tag="ACG8ZM_002555"
     CDS             252..1112
                     /gene="ylqF"
                     /locus_tag="ACG8ZM_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0493474.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            1105..1872
                     /locus_tag="ACG8ZM_002556"
     CDS             1105..1872
                     /locus_tag="ACG8ZM_002556"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0493475.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            1928..2785
                     /gene="dprA"
                     /locus_tag="ACG8ZM_002557"
     CDS             1928..2785
                     /gene="dprA"
                     /locus_tag="ACG8ZM_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0493476.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            2907..4985
                     /gene="topA"
                     /locus_tag="ACG8ZM_002558"
     CDS             2907..4985
                     /gene="topA"
                     /locus_tag="ACG8ZM_002558"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0493477.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            4954..6330
                     /gene="trmFO"
                     /locus_tag="ACG8ZM_002559"
     CDS             4954..6330
                     /gene="trmFO"
                     /locus_tag="ACG8ZM_002559"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0493478.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         2214 a         1123 c         1418 g         1621 t
ORIGIN      
        1 tctcctttct gttttgttcg tctatcatct cttatttagg aaacgctgtc aaaaatttag
       61 attcatatat agaaaccaaa ataaaaattt gcatcatcaa aaaagtgaag atgtgataga
      121 atgaggactg gttcactttc acttaactaa tagatggaat ttctttataa taaaaaagtg
      181 aatttatatt gaaaaagaaa aaaacaagta aagccgggga atattccggt atgaaaggaa
      241 tggagagaac aatgaccatc caatggtttc cgggacatat ggcaaaagcc cgacgagaag
      301 tttctgaaaa aatgaaatat gtggacatcg tatttgaatt ggtagacgca cgattacctt
      361 tatcttctcg taatccaatg ctagatcaga tcattcagca aaaacctcgc cttgttttat
      421 tgaataaagc ggatctggca gatccacaac agacaaagct gtggcaagaa tattttcgta
      481 aacaaggcca tcttgcatta gctatcacgg cacaagaaag taaaggaatt aaaggtctag
      541 tacctgcggc taaagaagca ttgaaagaaa aacgggcaag agatgctgct cgggggatca
      601 agcctcgcgc tattcgggca atggtcttag gtattccaaa tgttgggaaa tctacgttga
      661 tgaatcgact agtaggcaag aaaattgcac agacaggaaa taagcctggc gtaacaaaag
      721 gtcagcagtg gcttcgttta ggtaatgagt tggaattgtt agacacacca gggattttat
      781 ggcccaaatt tgaagatgaa gaaatcggca aaaaactagc gctgacaggc gcgatcaaag
      841 accagcttgt ccatttagat gatttagcga tttacggatt agactttttt gcccgatatt
      901 attcaggaag aatcagcgat cgttatggtt tatcaatcga gcaagaagaa aaattggcac
      961 cggaattact gatggatatt acagcaaaac gtgggtataa agaagactat gatcgtggca
     1021 gcgaattagt cgtgcatgat attcgtcaag gaaaacttgg acgatacaca ctggatcgtt
     1081 gcgaagaaat ggagcaaaaa agtgatgact gaatccatcc aaagtatcaa agaacgattg
     1141 aaaagcatac agagaataga tgatcctaca ctagaatcat taagaaatga tccacgcaaa
     1201 ggcgtgcagc aagcactggc ttcttttgaa cgacgattga aaaaggaagc attaattaag
     1261 gaaaaatatg aagaaatgtt ttgctttgaa aagcaagggt acgcacaagg tcaccaatgg
     1321 attgccggca ttgatgaagt aggaagagga ccattagctg gacctgtcgt ggcagctgca
     1381 gttatcttgc cgaaaaatga gatcatatta gggctgaatg actccaaaca attatcagag
     1441 aaaaagcgag aacttcttta tcaacaagtt caagaaaaag ccctggcaat cgggatcggg
     1501 atcgttgatc agcagacaat cgatgatgtg aatatttatg aagcaagtaa attagcgatg
     1561 atccaagcag tagaaaaatt gtccattgta ccaagctatt tgttgatcga tgcgatgacc
     1621 ttggatttgc ctattcagca ggaaaaaata atcaaagggg atgcacgatc tgtgtcgatt
     1681 gctgctgcaa gtatcattgc taaagtatat cgagaccaat tgatgaaaga atatgacgtg
     1741 ctttatcctg gatatggttt ctcgcataat gcaggttatg gaacaaagga acacttagaa
     1801 ggacttgaac gacaaggaat tacgccaatc catcgtaaaa gttttgcacc tgtaaaaaaa
     1861 tatttacaat aaaaaacagc cgcttttacg tatcttatta gaaagagaga cgaaaggcgg
     1921 ttttatgatg tatcaaatag aagaaaattt attgaaactt gcttatacaa aaggaatcag
     1981 tagtcaagga aaatgggcac ttgctaactg gatgatgcag tatccttata aagaaatcgg
     2041 atttgaagac atcattaaga taggtaaaat aaccaaacac cgggagctgt ttcgcaaaag
     2101 ttggcaggaa attcacgcta actggggcaa aatccaatcg aaacagtcgt ttatcacttg
     2161 ctttgatccc aaatatcccc ctcagctgct tcacttgacc tatccgccaa ttgtactgtt
     2221 ttataatggt gatctatctt tactttcttg taacatgtta agcgttgttg gtggaagaca
     2281 cacttctgct ttagccaaaa aaacagttta ttatttacta aaaccagtga tagaagctgg
     2341 atatgtgatc gtcagtggct gcgcgaaagg gatagataca tacggtcacc aagcagcaat
     2401 ctctcatggt ggacggacaa tcgcagtcat tggaacaggg atagagcaag tctatcctgc
     2461 tgaaaatcgt gacttgcaag aggtgattgc aaatgatcat cttcttttga gtgaatatcc
     2521 tcctcaatcc ggcccgaaaa agcattgttt tccaatgaga aatcggatta tcgcagcttt
     2581 gtctaaagga acatgtgtct ttgaagccaa gaaaaaaagc ggctctctga taacagcaca
     2641 gcaagcattg gatcttggaa gaacagtttt ttctgttcca ggaaatattt taacagaaca
     2701 ttctaccgga tgtcatcatt tgatccaaga cggtgcagtt tgcacggttt cgggtgaaga
     2761 tatcttggca gaatttaaag aataacacca tacttttcat ttgcttttct tgacaaacgt
     2821 tttctgaatg gatatgatta gctacgattt gtatgaatcc tcttttaata gtaggaacac
     2881 gacaatggaa aggagtccat tcagtaatgg cgtataaata tttagtgatt gtagaatcac
     2941 ctgcaaaagc caagacaatt gagaaatatc ttggacgaaa ctataaagta atggccagtg
     3001 ttggccatat aagagattta ccgaaaagta aaatgggtat cgattttgaa aacaattatg
     3061 aaccccatta tatttctata cgcggaaaag gcgatgtcat caaaagcctg aaagccgcag
     3121 caaaaaaagc tcaaaaagtt tacttggcaa gtgacccgga tagagaagga gaagcgattg
     3181 cttggcattt agcgtaccta cttgggttgg atctgaaaga aaaaaatcgg gtggtcttca
     3241 atgaaatcac aaaagacgca gtcaaagcag cttttaagga accaagaacg atcgatgtag
     3301 atttagtaga tgcacagcaa gctcgtcgta ccttagacag aatcgttggt tattcgatca
     3361 gtcctattct ctggcgtaag gtcaagaaag ggttaagtgc aggacgtgtc caatctgtcg
     3421 ctttaaaaat tattattgac cgtgaaaaag agatccgaga atttgttcca gaagaatatt
     3481 ggagcatcga cggtaatttt aaaaaagctc gcaagaaatt caaagcaaat ttctggggaa
     3541 tcgacggtaa gaaaaagaaa ttaccagatg cacaaagtgt aaaagaagtc actgctagaa
     3601 ttgatggaac agacttcgaa gttaaaaaag tagaaaagaa agagcgcaaa agaaaccctg
     3661 cgaatccttt cactacaagt agcctgcaac aagaagctgc ccgtaagtta aactttcgaa
     3721 caagaaaaac catgatggta gcacagcaat tatatgaagg aatcactttg ggtaaacaag
     3781 gaacagtcgg cttgattaca tacatgcgta ctgattctac ccggattgcc gatacagcaa
     3841 aagcagaggc cgcagcattt attgaagata cgtatggaaa agaattttct agacatgatc
     3901 accgaaagat caaaaatgct caaggcgcac aagatgccca tgaagctgtg cgtccaacga
     3961 gtgtgcttcg tacaccagat gaactcaaac aatatttaga taaagatcaa ttgaaacttt
     4021 atacattgat ctggtcacgt tttgtggcta gtcagatgac accagctatt ttagatacaa
     4081 tgaaagtgac attagaacaa aatggtgtca tcttcattgc gaacggttca aaaatcaaat
     4141 tcaaaggatt catgcaagtg tatgtggaag gtagagatga cggcaaagaa gagaaagaaa
     4201 atattcttcc agaacttgaa gaaggcgatg tagtccaatc tgttgatatt gaaccaaaac
     4261 aacattttac tcaaccacca gcacgtttta gtgaggctac gttgattcgt gcactagaag
     4321 aaaacggggt agggcgacct tccacttatg caccaacact tgatacgatc caaagacgct
     4381 actatgtaaa attgacacaa aaaagatttg agccgactga actaggagaa attgtcaatt
     4441 cattaatctg tgaattcttc cctcaaatcg tcgatatcca tttcacagca gaaatggaag
     4501 gagatttgga taaaattgaa gaaggcacgg aagcgtgggt caaagtggtt gatcgtttct
     4561 ataaaccgtt tgaaaaagaa ctgacgaatg cggaagaaaa aatcgaaaaa attcaaatca
     4621 aagatgaacc agcaggattc gattgtgatg tctgtggaca tccgatggtg ataaaattag
     4681 ggaaatatgg gaagttttat gcttgcagta attttcctga ttgtcggaat acgaaaccaa
     4741 tcgtaaaaga aatcggtgtg acttgcccag tatgtcatga aggacaagtc attgaacgaa
     4801 aatctaagaa aaacagacta ttctatggtt gttcccgttt cccagaatgt gaattcactt
     4861 cttgggataa accagtgggc agaaattgtc caaaatgtga cggatatctg gtcgagaaga
     4921 aagtcaaagg cggtaaacaa gtagtctgtg taaatggaga ttacgaagaa gacatccaaa
     4981 aataatagaa aagaggttct ttcattgaaa atggtaaatg ttgttggagc agggcttgcc
     5041 ggaagtgaag ctgcttggca gatcgcacaa gcaggagtcc ctgtccgttt atatgaaatg
     5101 cgtccagtca aatcgactga ggcgcatcac acaagtaatt ttgctgaact agtatgttcc
     5161 aattcattaa gaggaaacag tttggctaat gcagtagggt tattgaaaga agaaatgcgc
     5221 cgattgaatt cggtagtaat cacttcggca gatgaaacag cagtgccagc tggtggtgcc
     5281 ttagctgttg accgcgattc tttttctgaa acaattaccc gaaaagtcaa agagcatcca
     5341 ttagtaacag tgataaacga agaaattact gaaataccgg aaggaatcac cgtagtagca
     5401 acaggacctt tgacttctca tccattagca gaaagcatca aagcattcaa tggttcagat
     5461 ggtttttatt tctatgatgc agctgcacca atcgtagaca aagctacgat cgatatggat
     5521 aaagtatatt tgaagtcccg ctatgacaaa ggagaagcag cttacttgaa ctgtccgatg
     5581 acagaagagg aattcaaagc tttccatgag gcactaatca atgccgaagt ggcagaactt
     5641 aaatcttttg agaaagaaaa atattttgaa ggatgtatgc caattgaagt aatggcacag
     5701 cgaggattca aaacgatgct tttcggacct atgaaaccag ttggattgga agatcctaaa
     5761 actggcaaac gtccttatgc tgttatccag ctgcgtcaag acaatgctgc agcttcttta
     5821 tacaacatcg tgggctttca gactcacttg aaatggggcg agcaaaaacg tgttttccgg
     5881 atgattcctg ggttagaaaa tgctgaattc gttcgctatg gtgtgatgca ccgaaacagc
     5941 tttatgaatt cgccagaatt gctgaaaccg acttaccaat caacgaaacg cgaagatctg
     6001 ttctttgcag gtcagatgac tggtgtagaa ggctatgttg aaagtgcagc gagcggacta
     6061 cttgctggaa tcaacgccgc acgcttagca aaagaaatgg aaccagtcga gtttccacaa
     6121 gaaacagcta ttggaagtat ggcttactac attactcatg cggaaggaaa acatttccaa
     6181 ccgatgaatg ccaactatgg tctgtttcct gaactgccag agcgcattcg agacaaaaaa
     6241 gcaagatatg aagcaattgc acaacgagct ttaaaagcta atgaggcaat aaaaacagaa
     6301 ttagaaaaaa ctgcaatccc aaatgagtaa ggaaactcca tgaagagggt gagacggata
     6361 gtctcgctct ttttct
//
LOCUS       DAZKOR010000118         6347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000118 DAZKOR010000000
VERSION     DAZKOR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.121"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1135
                     /locus_tag="ACG8ZM_002560"
     CDS             290..1135
                     /locus_tag="ACG8ZM_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0493479.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="ACG8ZM_002561"
     CDS             1416..2636
                     /locus_tag="ACG8ZM_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0493480.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="ACG8ZM_002562"
     CDS             2633..5353
                     /locus_tag="ACG8ZM_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0493481.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="ACG8ZM_002563"
     CDS             5337..6281
                     /locus_tag="ACG8ZM_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0493482.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2363 a          983 c         1224 g         1777 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttc
//
LOCUS       DAZKOR010000119         6230 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000119 DAZKOR010000000
VERSION     DAZKOR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.122"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..633
                     /locus_tag="ACG8ZM_002564"
     CDS             133..633
                     /locus_tag="ACG8ZM_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0493483.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="ACG8ZM_002565"
     CDS             730..2577
                     /locus_tag="ACG8ZM_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0493484.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="ACG8ZM_002566"
     CDS             2580..3476
                     /locus_tag="ACG8ZM_002566"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0493485.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="ACG8ZM_002567"
     CDS             3526..3915
                     /locus_tag="ACG8ZM_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0493486.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..>6230
                     /locus_tag="ACG8ZM_002568"
     CDS             3902..>6230
                     /locus_tag="ACG8ZM_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0493487.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2106 a          925 c         1336 g         1863 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag cgaaacaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DAZKOR010000120         6068 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000120 DAZKOR010000000
VERSION     DAZKOR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.123"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACG8ZM_002569"
     CDS             832..1566
                     /locus_tag="ACG8ZM_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0493488.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACG8ZM_002570"
     CDS             1582..2751
                     /locus_tag="ACG8ZM_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0493489.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8ZM_002571"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8ZM_002571"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0493490.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACG8ZM_002572"
     CDS             3841..4767
                     /locus_tag="ACG8ZM_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFQ0493491.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACG8ZM_002573"
     CDS             4786..6036
                     /locus_tag="ACG8ZM_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0493492.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2026 a          997 c         1247 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAZKOR010000121         6049 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000121 DAZKOR010000000
VERSION     DAZKOR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.124"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..114
                     /locus_tag="ACG8ZM_002574"
     CDS             <1..114
                     /locus_tag="ACG8ZM_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0493493.1"
                     /translation="SSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(200..1294)
                     /locus_tag="ACG8ZM_002575"
     CDS             complement(200..1294)
                     /locus_tag="ACG8ZM_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0493494.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1483..2244
                     /locus_tag="ACG8ZM_002576"
     CDS             1483..2244
                     /locus_tag="ACG8ZM_002576"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0493495.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2359..2799)
                     /gene="fabZ"
                     /locus_tag="ACG8ZM_002577"
     CDS             complement(2359..2799)
                     /gene="fabZ"
                     /locus_tag="ACG8ZM_002577"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0493496.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3019..3774
                     /gene="fabI"
                     /locus_tag="ACG8ZM_002578"
     CDS             3019..3774
                     /gene="fabI"
                     /locus_tag="ACG8ZM_002578"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0493497.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3884..4936)
                     /locus_tag="ACG8ZM_002579"
     CDS             complement(3884..4936)
                     /locus_tag="ACG8ZM_002579"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0493498.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            5062..5982
                     /locus_tag="ACG8ZM_002580"
     CDS             5062..5982
                     /locus_tag="ACG8ZM_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0493499.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1855 a         1203 c         1078 g         1913 t
ORIGIN      
        1 tccagtgtca gcaatcaacg taatcatatt ccaagaaaat cattgaatta cagaacacca
       61 atcgaaatat ttttgagcta tgtacaagaa gcattttatt ctagcttaat ttgacaaatc
      121 ataacctata aaaaaacaaa ataaaaaagc agaaataact ggctatttct agcggattat
      181 ttctgcaata actaattatc tattttttat tttcaattaa tttaaagctt gaccatggtt
      241 tcaaaaagtc aaggaggaat aattcgtcat cggagattct tcctacaaca ttgacacgtc
      301 cgtcgttttt catatccttc aaggcgattt gtgtttctcc tttgtattga ccgtaccctt
      361 cattgtcaat cagtacatcc ccatgatgca tatctcgcgt attatgaggc gggaaatctt
      421 tatctttgta gtatacacga gtcatcgttg aacgaaggat atattctgag cgatcccctc
      481 gataactatg aaggttatca aacaagcaga ttcgttcgtt ttctgtaata ccttcttccg
      541 tatctacttt caatgttgga taatccgcgt taaatgcttc tgccatttct ttcaattctt
      601 cttcacttgc gtacgcattt ccaatagaaa tatcatcaat caatcctgta aggattaaat
      661 gcttcacttg tgtcgcaatt tcaagatctc ggtggtcttc cagcgtgcac aagccatctt
      721 gcgtaggcca agggccaaac tcagcagatt gcgaattgac gaatgccatc gtattcaaat
      781 tatattttct gaatttttct gaacaaaaaa caaagtgttc gtatcctaaa ccagagtatc
      841 gatgaggata aaaattatga gaacctaata aattatccgt gtttggcgaa taactcatga
      901 tgttgtcaac gtaattcgtg cccgaactca tattgatctc gattttgata ccaaaaggat
      961 tgcgcgtcat ttttgcttct tctgctcctg tgaatccgat atctaagcgg accccgtagg
     1021 cacccatctt atggaaaaaa gataagtcat cgtaagaaat ctccaactgt tcaaataacg
     1081 ctggattgat atcaaccatt acttccatgc ctaggctatt cgcgtaatca actacttctt
     1141 taaattctga aagaactttt tctttatcat cattgatctg cagcaaactg gtaaaaaccc
     1201 gtttataccc atacttatga gctaaatcca agtacgcttt gtccttttca aatgttgacc
     1261 gttctggata aatcgagatt cctaattttc ccatattacc atttcctcct gcatcttttc
     1321 gtttttgtat ccacttacat tatagcaaac acaaagtaaa tggtctacac cttttctttt
     1381 tcttttaaaa taaatgataa attccaataa tttaagtagg tattttccct ctattttgtt
     1441 atcattaaag ataacaaaat agtaaattgg aaaggaacag ttatgaagaa aaataaagtt
     1501 attttaagta ttagtttagc agcagcattg ctgtttgctg ctggttgttc tgcaaacaac
     1561 acgacttctt ctagttcgtc tacagaaagt tcaaaaagta catctactgc ggaaagcacg
     1621 actgccagta gcgtcgattt gaacgcattg gaattaccac aattgacgaa agatgtcgcc
     1681 gaaaatgaag atctggtcga aatggtgacg aataaaggca caatcaaaat caaattattt
     1741 cccgaacacg caccaaaagc ggttgaaaac tttatgacac atgcaaaaga cggttactac
     1801 aacggactga ctttccatcg cgtcatcaaa gatttcatga tccaaggtgg cgatcctaaa
     1861 ggcgatggca ctggagggga aagcatttgg ggagaaggat ttgaaacaga gatcaacaac
     1921 cacctttata atataagagg tgcgttatct atggctcgct cacaagatcc taacagtaac
     1981 ggcagccagt tctttatcgt tcaaaataca gatgatatgc atgatggtct tttaaaagac
     2041 gactatcctc aagcaatcat cgacgcttat aaaaacggcg gttatccttc actagatggc
     2101 cattatactg tcttcggcca agtgattgaa ggaatggatg tcgtggatgc tattgcaaat
     2161 atcgaaacag atagttcaga caaaccaaaa gaagaagtaa aaatcgataa aatcaatata
     2221 ctgcaagaag caaaacaaaa ataaaaaaat aacgtctgaa tcctgcatag atgacaacgt
     2281 atgctaggat tcaggcgtct tctttttaca taaaaagcaa gtaagcttcc ttttgttttg
     2341 ctatatcagg ttttatagtc aagcattttg gttgacgatg aacgtgaact cgcactcaca
     2401 gactttttta tcatctacat atgctgcaga taacgctgta ccgactgatc ccttcatttt
     2461 ggtgatttca aaatgacatt tcagcacatc tcctggaaca actttttgac ggaatttggc
     2521 cttattgatc ccaccgatat aagctctttt tcctagaaac tcttctgatt ttaggataag
     2581 tatcgaacct gcttgtgcca gtgtttctag aatcaacgca cccggcatca ctggattgtc
     2641 aggaaagtga ccttggaaaa aatcttcatt gattgttaca ttttttgtcg caacgatctt
     2701 ttttcctggt tctaaagtat ctacataatc aatataacaa ataggataac gattaggaat
     2761 caattccatt acttcagtag cagataatac gtaagacata attaaactcc ttctttcagg
     2821 caattgccat tttcattact ttgactatca aagtatcata ttcctaaata tttagcaatc
     2881 tttttttaat ttaagaaaaa atagtttgac aatcaaaaca ttttgactaa taattaagaa
     2941 atcaagaagt tattttagct attattctct atttttttac gactatcaaa tttttatcag
     3001 aaacaaagga gagtcattat gtcatttcta gaaggaaaaa aaatcgtcat tatgggcgtc
     3061 gctaacaaaa aaagcattgc ttggggttgt gctgaagcaa tgttgaaaca aggggcagaa
     3121 attatctata cttaccaaaa tgaacgaatg aaaaaatcat tagtcaaatt ggtcggcgaa
     3181 gaagcattca ctgtagaatg tgatgtttct agcgacgaaa gcatcgaaaa agcaatgaat
     3241 gccatcggtg aatatgctgg ggagatccac ggccttgtcc atgcagttgc ttacgccaac
     3301 aaagaagaat tgtccggaaa tgtgtctgat atttccagag acggctatca attagctcaa
     3361 gatatcagca gctactcttt aattgctgta tcaaaatacg cacaaccgtt tcttgcacca
     3421 aacagcggga tcgttagcat gacttatcta ggttcagaac gcgcaatccc gaattataac
     3481 atgatgggta ttgcgaaagc ttctcttgaa gctgctatcc gttatttggc agctgagttc
     3541 tcgcctaaag gaattcgggt caatggaatc tcagctgggg caatcaaaac attagctgtc
     3601 acaggtgtta aagactatca aaaactgatc cagttatctg aatctcgtac gcctgatcag
     3661 caaggtgtga cgatcgaaga agtcggtaat gcttgtgctt tcttaatcag cccactttcc
     3721 agcggaatca tcggtgatat tatctacgtt gacaaaggtg tccatctttc ataaactttt
     3781 gttgcattct ttaaagtaaa acatataaaa atcaggaaaa aactttttaa agtctcttcc
     3841 tgattttttc tttttacgtt tttttcaact cattactttg aaattattcc acgacttcaa
     3901 tccagccttc tggtgcttct atgtctccaa attgaatacc tgtcaattca tcatacaacc
     3961 gttttgttac tggtcctact tccgtttcac tataaaatac atgaagattg ttattgtgat
     4021 aaatcccacc gattggagaa ataacagccg ctgtaccaca tgcaccagct tctgcaaatt
     4081 gatctagatc atcaatgaaa atatctcctt caattgcttg cagtcctagt cgttcttcag
     4141 ctaactgcaa caaggaatat ttcgtgatac ttggtaagat agagggcgat tgcggagtga
     4201 tgaattttcc gtcttttgta attccaaaga aatttgctgc acctacttct tcgattttcg
     4261 tatgtgtggc tggatcaaga taaacacaat ctgagtaatc cagttctttt gcttctgttc
     4321 ctggaagtaa actcgctgcg taatttccgc cgacttttgc tgcaccagtc cccttaggag
     4381 cagcacgatc gtaatcagat acaataaaat tcgttggttt tagtccacct ttgaaatacg
     4441 ctcctactgg cgtacagaag acgacaaata aatattctgg agctggatgg acaccaatgt
     4501 tttcaccgac cccaatcagt aaagggcgca agtataatgt agcgcctgtt ccatagggcg
     4561 gcacaaaatc ttcatttgct tttaccactt gcttgacagc atcaacgaac atcttttcag
     4621 ggaccattgg catcagcaat cgtctagcac tttgattcat ccgtcgacta ttttgttcag
     4681 gtcgaaagag attgaccttg ccgtcttttc gacgataagc ttttagacct tcaaaacatt
     4741 gctgaccata atgaagcgct ggagagcctt catgaatcgt caggtaatta tcttctgtca
     4801 attctccttc ttcccattgt ccgtctttcc atttggcaat aaaacgccaa ggagttttga
     4861 tataagaaaa acctagattg ttccaatcga tttgcatata cttaccacct tcgttatttt
     4921 ctccacattt tatcattatc catattattt gtcattttat tttcagcttt tttccttcat
     4981 tatagttatt tttgctattt ccttttttga aaacttcttg gtacaataca gaaagaatca
     5041 aattttagaa aagaggattt tatgttttca ttattagcaa ctgccggtac gacagactct
     5101 tctggcgaga cattgacaga tcaagctgtc agcaaagtaa atgcgtttca gcggttttgg
     5161 gacgggattg attgggacgc cattttggct gcctttatcc aaaaaagttt atatctgatt
     5221 tttttgatcg tccttttttg gattcttcac cgtgtgggaa aatatctgat tgataagtct
     5281 tttaatcagt attctaaaaa agccattcta aacgagagcc gcttaaagac tttacatagt
     5341 cttttaaata ctatttttca ttataccctt ggtttctttt ttatctatgc aattttatct
     5401 gctatcggtg tgccgatcgg atcacttttg gctggtgccg gaattgcagg tgtggcaatc
     5461 ggtcttggtg cccaagggtt catgagtgat gttattaccg gattttttat tatcatggaa
     5521 caacagatgg atgttgggga ctacatcaag ctagcaaact taacgattga aggaaccgtc
     5581 gtatctgttg gtatccgtac ccttcagctg aaagcaacgg atggtactgt ccatttcatc
     5641 ccgaatcgaa acatcaccac gatcagcaat ctctctcgtg caaatatgca agtactttta
     5701 gatattcgga ttgttcctga agaaggatat gacaaaatct atgagatcat tgatcgagta
     5761 aatcgtacat tagctgaaaa ataccaagaa gagctccaaa ctgaaccttc tatctttgga
     5821 ttagtggatc ttggaaacag caattttgcg attcgtacgg tctgctacgc attgaacgga
     5881 aaacaatatg ccttaaaaga agagttcttg agctcctatg tcaaagaatt gacagcttct
     5941 ggtttcacga ttccgaacag tccaattgct gtcggaaaat aatttggaca aacgatgcaa
     6001 cgaatctcac taaaaagagc gtgggacaaa agtaaagaac acttttgtc
//
LOCUS       DAZKOR010000122         6043 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000122 DAZKOR010000000
VERSION     DAZKOR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.125"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..571
                     /locus_tag="ACG8ZM_002581"
     CDS             <1..571
                     /locus_tag="ACG8ZM_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0493500.1"
                     /translation="YHLKFEKKKYVLQVFHPVVTELENIEKQILALINSMEQQSQPII
                     CILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAA
                     SFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDG
                     NSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            544..1221
                     /locus_tag="ACG8ZM_002582"
     CDS             544..1221
                     /locus_tag="ACG8ZM_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0493501.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1172..2353
                     /locus_tag="ACG8ZM_002583"
     CDS             1172..2353
                     /locus_tag="ACG8ZM_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0493502.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            2366..3343
                     /locus_tag="ACG8ZM_002584"
     CDS             2366..3343
                     /locus_tag="ACG8ZM_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0493503.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3343..4251
                     /locus_tag="ACG8ZM_002585"
     CDS             3343..4251
                     /locus_tag="ACG8ZM_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFQ0493504.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            4252..5004
                     /locus_tag="ACG8ZM_002586"
     CDS             4252..5004
                     /locus_tag="ACG8ZM_002586"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0493505.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            5009..5956
                     /locus_tag="ACG8ZM_002587"
     CDS             5009..5956
                     /locus_tag="ACG8ZM_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0493506.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         2224 a          763 c         1142 g         1914 t
ORIGIN      
        1 atatcatcta aaatttgaaa agaaaaaata cgttttgcaa gtatttcatc cagttgtaac
       61 cgaactagaa aatatagaaa aacaaatttt agctttgatc aattcaatgg aacaacaatc
      121 tcaaccaatt atttgcattt tacctaatag tgatgctggt agcgaaatta tcagaaaaat
      181 gtaccagaaa tatttaaaaa cggatattat ttatattacg aatttagagc cggaggaata
      241 cctgactata ttaaaagagt gcaaatttat gatcggaaat tcttcttctg ggataattga
      301 agctgcatca ttcaaaatac cagtaataaa tttaggaacc agacaaaatg gacgagaaca
      361 atctaaaaat attataaatg caaatctaaa tgaaaatgaa attgaaactg ctttgaataa
      421 aatacaagat cctcagttta taaaaagctt gaaaaatgtt aagaacatct acggtgacgg
      481 caacagttct agaagaataa caaaaacatt gaatcaaata ttgaatgatg gggaagatat
      541 taagtggatt caaaaacaaa tagcttatta aaatatacta gtgatccata cattattatt
      601 ggggctggcg gacacgcaaa agtcattatt gaaaccttaa gattagaaaa taagttaata
      661 tatggatttt ttgatgataa gcaaaataaa atgcaagatc tgaaatatct tgggaaaata
      721 aacgaattga atagctgggc ggatagcaaa aaaaaaatct ataaatttat tgtagctatt
      781 ggtgactgtg tagtaagaaa aaagattata aaaaaattga atatgccaag tgagcaatat
      841 ggaatagctg ttcatccaag tgctatagtg gcaaaagaaa tttcaattgg ttatggaact
      901 gttatttttg cgaatgctgt aattaaccca gatgctgtaa ttggagagca tgctataata
      961 aacacaggta gcattgttga gcatgattgt agaattaata actatgttca tttatctcca
     1021 ggtgtatgct tagcgggtgg agtacatgta ggtgtaggaa ctcaaatagg aattggttct
     1081 caatgtatcc agatgaagaa aattggatct tggtgtatga taggcgcagg gagcactatt
     1141 gttaaggata ttccctcaca ttccttagct tatggcaatc cagctaaaat taagaaagaa
     1201 ggaattaatt ttgaattcta gaattttatt atcaagtcct catatgggag gaacagaaga
     1261 aaaatatgtt gctaaggctt ttaaagataa ttggatagcg ccattaggtg aaaatgtgga
     1321 taaatttgaa gaaagtatta caaaatatac tggttctaag ggcggagctt ctgcgttaag
     1381 ttcaggtaca gctgccatac atcttgcctt aagactgctt aatgttggaa aaaacgatat
     1441 tgtctttgct ccttcattta cttttatagc tacagtaaat cctattttat atcaaggggc
     1501 tgtaccagtg ttcattgact cagaacctaa tacatggaat atgagccctg ttgcactaga
     1561 gagcgcatta gaagaagctg aaagaaacaa ccaattacct aaggcaatca tcgtagtgaa
     1621 tttatatggt caaatggctg attatgaacg aattttagct attgcaaacc aatttaatgt
     1681 tccagtgata gaagatgcag cggaatcgtt aggttctact tataagggaa tttcgtcagg
     1741 cttatttggg acaattggga tctattcatt taacggtaat aagatcatca caacctcagg
     1801 tggaggagct ctagtgtcta gtgataaaaa atatgttgag cattcaagat ttttagcaac
     1861 acaagcaaga gatcaagcac cgtattatca acatagcgaa ataggttata attaccgtct
     1921 aagcaatatt tctgccggta tcggacgtgg acaaatggaa gtattgaatg atagagtagc
     1981 taaacgaaga gaaattttca atcgatataa ggaagctttt tctggaattg atagaattga
     2041 aatgatgcca gaagtggatg gcatggtgcc aaatcgttgg ttgtctactt taacgatcaa
     2101 tgattataat tctattgatc aagtagttaa cgtaataaat aaactaaatg aaaaaaacat
     2161 tgaaacaaga gctttgtgga aaccactaca tttgcaacca gtatttcaag gggtaaaaat
     2221 gtattaccat gataataatc ttagtgtgtc agaaaagtta tttaatagcg gcttatgctt
     2281 accttctgga agtaatatga cagatagtga acaagaattt gttattgatg agtttaaaaa
     2341 aatattcaag taggtgtgat attttatgaa cataggaatt attggtttag gttccatggg
     2401 gaaaagaaga cttagactca ttcaagaaca ctttaatgat attttactat gtggtgttga
     2461 tactaatgag gcgagacgaa aggaagttga aaaggaattt ggaataccaa cttataatag
     2521 tctaacagag gcaaaaaatt tgtttgaaat aaattcagtt tttgtttgta catcccctat
     2581 aacccatgaa tttattataa aagaggcttt aaatttagat tgtaatatct ttacggaaat
     2641 caatttatta aatgaatatt atgatgaaat aatcgaagta gctaaaaaga aagatagatt
     2701 actttatcta tcttctacat ttttgaagag aaaagaaatt caatatatta agtctaaagt
     2761 gacgcataat aaaaaaattt cttatcaata ccatgtagga cagtatttac ccgactggca
     2821 tccttgggaa gattatcgca atttttttgt ttcaaacaaa aaaactaatg ggtgtagaga
     2881 aatattcgct attgaattac cttggattat agatacattt gggaaagtta aatcgtttac
     2941 tgtaatgacc cagaaattat ccaatcttga tattgattat tcggacagtt tcactcttat
     3001 tattgagcat gaaactggta tagttggaac tttaagtgta gatgttgttt caagagtagc
     3061 taaaagaaat ttaactataa ttggtgaaaa gattttaata gaatgggaag gtaacccgga
     3121 ttctctattt tcatggaatg aagaaacttc tagtatgaca gatattcaat tgtacgatga
     3181 ggttatgcat gataataatt attcacatac tattattgaa gatgcatatt tagaggagat
     3241 taaagaattt ttcacgcttt tggaaaagaa aatggaaccc caatatactt ttgaaaaaga
     3301 taagtatatt attaacttaa ttaacgacat tgaggtaaat taatgatgaa agtaattttt
     3361 tttgggttag ggtcaattgg aaagagacat ctaaaaaatc taataagcta tggaaaaaag
     3421 aattctatta attttgatat aacagcttat agtagcagtg gaaaaaaaga gtttgaagaa
     3481 ataacgtata ttcaaaatag agacgatttg gatacacatt atgatatagc ttttattaca
     3541 aatccgactt ttaagcatct ggaaacacta aatgtaattg ttgaaaaagt tgattacata
     3601 tttttagaaa aacctgcttt ttctgagagt attgatctta ctcaatataa ttatgattac
     3661 aaaaatattt atgttgctgc tccactgagg tataaaaata tcatggaagt tgttcgtaat
     3721 agtattcaaa gtaaaaaagt atattctgta agagctattt gttctactta tcttcctgat
     3781 tggagaaaag gtactgatta tagaaataat tattctgcct ttaaatctat ggggggagga
     3841 gtagaattag actgtataca tgaattggat tatattataa acctatttgg tttccctaaa
     3901 tcagtaaaaa aacagtttgg taaatattcg aatcttgaaa ttaacagtaa tgatttagca
     3961 acatatatat tgagatacga tgataaaatt atagaaattc atttagatta ttttggaaga
     4021 gttcctacaa gaaaatttca attgtttact ttagatgaat gtatcaatgt tgacttatta
     4081 aataataaaa taagttttga agaaaacatt gttaaagaaa tgcctgaatc tgctaatgat
     4141 atgtatgaga aggagttgaa atatttcttt gaaaacgtag taacaggcaa agaaaactgg
     4201 aatgatttat atcatgcaaa cgaagtattg aaaatagctg aggagaaata aatggaaatt
     4261 ttatttacta tttgtggtag agctgggtct aaggggttta aaaataaaaa tttgaaatat
     4321 ttggcaggaa agcccctagt ccatttcgct ttagcaatga tagatatcta tcaacaaaaa
     4381 catcctgaac ataattgcac gacttgtttg aatacggaca gtaatgagtt agtagaattg
     4441 acgactaagt taaatccgtc tgtgaaaatt gtagaacgta aacctgaatt agctacagat
     4501 actgtaggga aaattgaagt gatacgtgat tcattcttac aactagaaag agaagataat
     4561 atatttgatt taattataga tttagatttg acttcacctt tacgtgaagt ttctaatctt
     4621 gaagaattaa tagatgaaca tctaaaaaat ttagataaag acgtctactt ttcagttgtt
     4681 tctgcaagac gtaatcccta ttttaatatg gtaaaaagaa ttgaaaatga agttactata
     4741 gttaataaat caagatacac atctaggcaa cagacacctg aagtttttga aatgaatgcc
     4801 tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta attatatttt tgatgggcaa
     4861 tgtgggataa taaaaatgag agattatcta attttagata ttgatagtga agaagatttt
     4921 aaatggttag aatttttgtt tcctaaattt ttagaagatt cttcggagct tcaagatatc
     4981 tataattatt tatatcggag gtaaaaggat gagctgttat cttatcaccg gtggggctgg
     5041 cttcattggt tctaccttag ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga
     5101 tgacttatca atgggaaaag tagaaaatct tgatatgacg aaaaatatca cttttattga
     5161 gggagatgtc gctgagaaat ccttgatgga agagatcatg aaagctaata aatttgatta
     5221 tatctttcat ttagcagcag ttgctagtgt ggcagattca gttgcccgac cagtagaaac
     5281 acatcgtgtg aattttgaaa gtgtattaat gattttagaa ttgatccgca agtatcaacc
     5341 tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt tatggggatg aaccgacttt
     5401 accaaaaaaa gaagagtccg tgattcgccc attgacgcct tatgcggttg ataaattcgc
     5461 tgcagaacaa tatgtgctag attattgtca tttatatgat gtacccggta gcgcagttcg
     5521 ttttttcaat gtctatggtc caaatcaaaa tccgaattct ccatacagtg gtgtgatttc
     5581 tattctcgta gatcgctata agaaacaaat cgtaggagaa gcaacttcat ttacattatt
     5641 tggtgatgga agccagtcta gagactttgt ttatatagat gatgtaatcc aagcattact
     5701 tttagttgcg aatgaggaag ctgcactggg gaaacaattc aatgttggaa cgggtaaagc
     5761 aaccacactt ttggaattga ttcatgcaat caatgaaatt ctcggtgtag aattgacact
     5821 tgaatacaaa gaagagcgtt cgggtgatat ccatgactct ctagcagata ttactaagat
     5881 ccagtcaatt ggctatcaac cgaaatatgg tattttaagt ggaatgacta attatttgaa
     5941 aactgaaatc aattaaaaca tactaagata agattagagg gtaaattcag gctctttgtc
     6001 aataaggact gatgagtttt gtaaaatcaa atctgggata gaa
//
LOCUS       DAZKOR010000123         6023 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000123 DAZKOR010000000
VERSION     DAZKOR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.126"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..501)
                     /gene="yajC"
                     /locus_tag="ACG8ZM_002588"
     CDS             complement(121..501)
                     /gene="yajC"
                     /locus_tag="ACG8ZM_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0493507.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(598..1743)
                     /gene="tgt"
                     /locus_tag="ACG8ZM_002589"
     CDS             complement(598..1743)
                     /gene="tgt"
                     /locus_tag="ACG8ZM_002589"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0493508.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1961..2659
                     /locus_tag="ACG8ZM_002590"
     CDS             1961..2659
                     /locus_tag="ACG8ZM_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0493509.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2827..5466)
                     /locus_tag="ACG8ZM_002591"
     CDS             complement(2827..5466)
                     /locus_tag="ACG8ZM_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0493510.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         1694 a         1323 c         1105 g         1901 t
ORIGIN      
        1 acacacccga ttattcaata ttgagcaata tatcccatgc tcttatctct ttcttttttg
       61 aataaaaaat gaggtaggac atctttctgc cctacctcgt ctttaaataa gctttgatac
      121 ttatttttct tccttctctt ctgtctcttc cagcgtttct gttgtttcag tttttacttc
      181 aactgtctca gctggttctt ctgttgtttc aacagtagtc gttgcatcat ttgttacgac
      241 agtccctggt ttgaccgttt tgattgctgc acgatcgtac tcaaggaaga tcccttcgca
      301 gtctaaaaca actgtccgtt tgtcattgtc gatttctgaa acaactccat gtaatccgcc
      361 gatcgttacg actgaatcac ctggtttcat gctatctaac aagttttgac gttcttgctg
      421 ttgtttcttt tgcgaacgtg acataaaaaa ccacattgct aatagcgcga taaacatcac
      481 aagcattggt aatgttccca tttttttcac ctcactaatt ctgtatctac aaaaaacact
      541 ttagcagaaa aatgctgata acactagttt tttacttaat ttttaaaaga ttttccttta
      601 gaagcttttt gcgttttctt tattgaaccc gtattcttca aaaaaggctt ctctaaattc
      661 aagcaaatta tcatccatga ttgcttgacg tacttgtttc attagattca acaagaagta
      721 taaattgtgg taagaagtca aacgaatccc aaatgtttca tcacatttga tcaaatggcg
      781 gatataggca cgtgtatagt ttttacatgt ataacaatca catttctcat caattggacg
      841 gaaatcatgg gcatatttcg catttttcac tactagacgg ccttgagaag tcatacatgt
      901 gccatttcga gcgattcgag taggcaagac acagtcaaac atgtctactc cacggataac
      961 accatcgatc aatgaatcag cagcaccgac acccatcaag taacgtggtt tgttttcggg
     1021 gatcaacggt gtagtgaatt ctaatacacg attcatttct gatttaggtt ccccgacaga
     1081 taacccgccg attgagtaac cagggaaatc catcccgact agatctttcg cgctttgacg
     1141 acgaagatct tcaaaccctg ctccttggat gattccaaat aatccttgac ggtctggatt
     1201 agcatgagct ttcaatcctc gttctgccca tcgagacgta cgttctactg attttttcac
     1261 gtagtcgtaa ctttcatcga atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc
     1321 caatttattt tggatgttga ttgctttttc tggtgataaa aacatacgcg aaccgttcaa
     1381 gtgatttttg aaatgcacgc cttcttcttc gatattccgc atatcactta atgaaaaaac
     1441 ttgaaagccg cctgaatctg ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc
     1501 gccagcttct tctactaagt cttcaccagg gcgtagccat aaatgatatg tattacttaa
     1561 aataacgcca gcacccattt ctttcaactc ttctggggac attgttttta ctgtagctaa
     1621 tgttcctact ggcatgaaca ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc
     1681 taaacgcgcg ccagtatgtt tttctttctt gattaaacga tagcgaatgg caggttgcgt
     1741 cattcatttc cctactttct atataaaata tcttgacaac tcccaccatc ataatcgttt
     1801 caagtgcttt ttgcaagttc cctcggcttc cttctacttt atttaatatt ctttttttta
     1861 tccttttttt atgaataaca aaaaaaagaa aaacattatg acagaaatat tttttgatac
     1921 atggtatatt ggtgttatta aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt
     1981 aaaacgccaa gcaaaagaga gtctaaaagg acgttgggga caagctgttt tactaaatct
     2041 aatccctaca ttgattggta ttgctactat tcttcttatt atgattccta ttatcttgtt
     2101 tgccaccacc atatatgatg gatcagcgag cgtctcagaa gcgacaactt caggaggcgg
     2161 tagttcagga ggcggtggcg gaattatttc cagcatcatt agcgctttgt tcatgagcgg
     2221 tatttcttgg acttatcttg atattttacg tggacaaaaa gatcagatcg aaccattcaa
     2281 agatgcattt cgtggattca aaggtttgtt tatcggcgga gtgatcagct tagctgtgct
     2341 gatctcgatc tttacaacat tatggggttt gctattagtt attcctggaa tcattaaaag
     2401 ctattcttat tctcaaagct attttattta ctacgatatc gtccaagaaa caggacaaac
     2461 acctaaagta ctggatacga tcactgcaag tcgaaaattg atggatggtt ataaaggaca
     2521 acttttctgg ttagatcttt catttatcgg ctggcatatc ttagcattag caactgctgg
     2581 tattggttat ctatggctca gcccatacat ttctgcaaca aaagcagcat tttatgaaca
     2641 attgccaaaa gatatttaaa agagagtgtg acttgtgacc gagggggtgg caggagaaca
     2701 ccgtttatcg agaggcagta aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca
     2761 acattcatgt cacacctcct ttttgtatag ttccagtaaa aagaaaatca acttagaaac
     2821 agttaatcat tcctcttctt ctgcttccgg taatcgatgg aacttgatca gttcattcac
     2881 cgcgattggt acgatagata aaatccctac caagaaccag tgattcaaac tgatgtgtac
     2941 taggccgaat agttcttgcg taaatggcag caaagcaaca gccgttgtaa tagctaatgc
     3001 cagtaacgcc atttctacca aagctttatt ggtcgctaaa ttgactttga agatcgattg
     3061 gctgctgcgt acattgaaca catgcagaat cgatgaataa gctaagatca agaatgcaac
     3121 agtttgaccg acatgctgac ttgcgtctac ccatgggctg atagtatcga cataactacc
     3181 taaataataa ccaaacaagg ttacgatggt gaagacccct gcatttatgg caattttttg
     3241 caataaacct cgagcaaaga tcccttcatt tttcggaatg ggtggctgat ccatgatgcc
     3301 gtgttccgct ggctctcttc ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac
     3361 aaacagtagt tgcacagctg taaatggtgc tccccatcct aacagcatcg caatcaaaac
     3421 aataaagatc tccgaaatat tacagcttag caaaaagttg atagctttac gaatattctg
     3481 ataaaccgaa cgtccttgac tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa
     3541 aatcatatct gcggcacttt gcgccacatc tgttcctgtg atccccatgg cacacccaac
     3601 gttacttgct ttcaacgctg gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc
     3661 acccgtacgt tgccaagatt tgacgatccg gatcttgtct tctggcgtta cccgagcata
     3721 gacagagaga gctttgacac gcgcatctaa ttcttcgtct gataattgat gcagttctga
     3781 tcctgaaagt gcttctgacg gatcattcaa gatacctaat tctttcgcta tggcactggc
     3841 agtcaccaca tgatctccag tgatcatgac tgttttgatg ccagcctttt tcgctcgctt
     3901 gattgctcct ttactttctg gacgtggtgg atcgatcatt ccgatcagac caatcaaacg
     3961 taagtctttt tccagtgctt cagaagaaat atcttgcggt tcttcgtcat aagtagcata
     4021 accaacagca atcactctca gtgcccgttt accaaagcga tcattaacga tcgccgcttg
     4081 ttctacatcg cctgaactaa agcgcggagc aagaacatcg aatgctcctt tggtgattga
     4141 gatgtatttt tcgcccattt ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg
     4201 aagctcgttt actcgtggat atttttcttc taattctgcc tttgtatgat aattctcttc
     4261 aacagccctt acgatggcta cttctgttgg attacctttg ataacaaggt cgtcatcttc
     4321 ttgctcgacc gtcacatctg tacatagtgc agccattttc aatatttcca tcgcgctgtt
     4381 cgtcattgaa tcttcgatat tcgttacttc atcttctttg ctccataccc ggcggacgcg
     4441 catttcattt tgtgtcagcg ttcctgtttt gtctgagcag ataacattag cggttcctag
     4501 cgtttcaact gctggcaact gtcggatgat cgcatgtttc ctcgccatct tctgcacacc
     4561 gtatgcaagt gtcaacgtaa caataacagt caaggtttcc ggtacagctg caacagccaa
     4621 ggaaatcgaa gtcatgaaca tctcaaggag tggttctcct tgcagttcac caatcacaaa
     4681 tactaaagcc gcagccgcta aagcgatcat actgattcgt ttgcctagct gattcaatcg
     4741 gacctgtaaa ggtgtttttt gcatagtatt ttcgttcaaa agatccgcga tcttacccat
     4801 ttctgtagcc atgccaatgg cagtgacgat cgctttccca cgacctgcag caaccgtaca
     4861 tcctttaaat accatattaa gttggtcacc taatgactca tcttcttcag caataaatgc
     4921 tgcatctttt tcaactgctt cactttctcc agtcaacgcc gattcttcta cacgtaattg
     4981 tgatgcttcg atcaaacggg catcggcaga aatagcatca ccggactcta aaacaagaat
     5041 atcacccact accagttgct ctgactcaac agtggtcact ttgccgtctc gtatgacagt
     5101 tgccgtttgt ttattcatat cttgcaagga ttccagtgct ttttctgcgt ttccttcttg
     5161 gacaatcgcc aataccgagt tgatcacaac gatagcgatg atcagcagtc cttcaaataa
     5221 atccccatgt tctgtagcaa atgcggtata aaaggaaatc accgctgcta caagaaggat
     5281 gatcgttgta aaatcggaca agcttcgaaa gaattttttg atcatcgatt ctttcggcgc
     5341 ctcatcgaat ttattcaatc cctgcttttt tcgccgttca tccacttctt gtgaagacaa
     5401 cccagtatct ggatctgctt ctgttacctt ctttacttct tctattgtct gcttgtattc
     5461 ttccatccta ttcctccttt aaaatcttcg tacaaaaaaa ggtacttatt ttactataac
     5521 atcctttttt tatttacttc aaataaaggg cttatcgatt atttgttaag aaagcgtaaa
     5581 atttaaagtg cttatatttg caaacgctac caaaggatgc tattatatag atgtaaggaa
     5641 ggaggatacc atcaaataga cagaagaatt tctcgataga acagtgtaag caaacaaagt
     5701 tgttagaagt acaggtttca cccagacatg cgtgtagagt acaaacctat aaatagtgaa
     5761 tgcgtatgga tgtttggatc acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa
     5821 ttgaataacg taaacgtctg aaaattgaat aggaacaaga gatgtttcaa atgttgaaga
     5881 tgtaacgaaa acaaaatttt tgtttaacat gaagaaattc aacaccgtgc tttgcatgta
     5941 tgatggaaag ttacaatcat ttctgtttat ttgaatcctt ccgaggtaca agaaagccga
     6001 gcttatcgag agctcggctt ttt
//
LOCUS       DAZKOR010000124         6020 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000124 DAZKOR010000000
VERSION     DAZKOR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.127"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..362)
                     /locus_tag="ACG8ZM_002592"
     CDS             complement(60..362)
                     /locus_tag="ACG8ZM_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0493511.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(582..948)
                     /gene="ssrA"
                     /locus_tag="ACG8ZM_002593"
     tmRNA           complement(582..948)
                     /gene="ssrA"
                     /locus_tag="ACG8ZM_002593"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1119..1892)
                     /locus_tag="ACG8ZM_002594"
     CDS             complement(1119..1892)
                     /locus_tag="ACG8ZM_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0493512.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2036..2386
                     /locus_tag="ACG8ZM_002595"
     CDS             2036..2386
                     /locus_tag="ACG8ZM_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0493513.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2367..3083
                     /locus_tag="ACG8ZM_002596"
     CDS             2367..3083
                     /locus_tag="ACG8ZM_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0493514.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3083..4531
                     /locus_tag="ACG8ZM_002597"
     CDS             3083..4531
                     /locus_tag="ACG8ZM_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0493515.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4602..5111)
                     /locus_tag="ACG8ZM_002598"
     CDS             complement(4602..5111)
                     /locus_tag="ACG8ZM_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493516.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5101..5825)
                     /locus_tag="ACG8ZM_002599"
                     /pseudo
     CDS             complement(5101..5825)
                     /locus_tag="ACG8ZM_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2045 a         1050 c         1170 g         1755 t
ORIGIN      
        1 cccttttata agtatggtga taaaatccgt tccgggctac cttttttagg tagcctactt
       61 taatctttat acctttctgg atcaacgaaa gtatacttca aatagtcata acgccgatga
      121 tcgctccgtg cgtctggcac atcagtcacg atatcaaaca aaaaatacac atctttcttc
      181 attctggttt ttgttgccgg aattttaaag tagttcttat tagagtaata gagattgatt
      241 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
      301 ttgacaaatc tattcgaagg atcaaattta aaccataatt gtgtttttcc ttccatcaac
      361 ataatgttca cctcaaaaag agtatacgaa ctaatgttct ttttgtaaag taggaatatc
      421 attgataata tggatggaac atatatatta aaaagtgttc tttttgaatg ctattttgaa
      481 tgccattttc attcatttaa cgaaaatctg cgaaaataaa aaatgctcaa acccttataa
      541 aataaggatt taagcatcta gcgaaatctg ctgaaatctc acaatggaga cggcgggagt
      601 cgaacccgcg tccaaacaca tcgccactta aaaatctacg ttcatagtca actcatttaa
      661 ggattcactt tacagcttgc cgagtgacag gcattctgta tcgctagtct gatgttctct
      721 ttttcaactt acagacggaa agttgaaacg tatcccacta aagttaggac ccttactcga
      781 gcacatgggc gatgccgaga ggatctacgc taactgtttt taggcagcta aagcgtaaga
      841 gttgttttcg tttttagcag ttatatttaa ctgtaacgtt tttacgtaga cgtaacctac
      901 gaaacgcaat tcaagctcga ctgtgcctgt cgaatccgta acgtccccgt tagctaacaa
      961 gggttacgtg tatttttcat acaaaattag tatagcatat attgggataa attcaataaa
     1021 aaagcgaatc atatcccatt atttatataa taactgcccc tttcttaaga aaaaataagc
     1081 gattttctta agaaaagagg tagcttcaaa aaaagatctt aaatcagatc aaaatacttc
     1141 aatgcgtgga caattccgcc ttctgtattt ttctttgtga taaaggtaga aagttctttt
     1201 aatccgtcat aggcgttgcc catagcgatt ttgttgtcgc aagcttccaa caaggcaaaa
     1261 tcattcggac catcgccaaa tccaaaagta ggaacttttt ccagctgaag ttctttttgc
     1321 agacgtttga cacctgcccc ttttgagatt cctttcttca ctgtatcgat cgagtaagga
     1381 ccattccgat aaaaagttag ctcaggaaaa tgttcgtgat agtattcgtc acctgattga
     1441 gataagatca gcaacatatt tactgtgttt tctttaaagc ctaaaggatc aatttccgga
     1501 acatctgaat gaatgaagtc gtaagcattg atcattgttt ggctatgacc ggtacaccag
     1561 attttctgat cgttatagta agaaagttca tgaccgttca actgtacatg atcatacatt
     1621 ttttgacatt cttctgaaga aaatgtttga gaatagattt cttcgccgtc tactcgaatg
     1681 aaagaaccat tcatggtgac cgcagaagta atgcccgcat cttccatgat agagtggatt
     1741 tctgcttctg tacgtcctgt agcgatgacg gggagaacat tgttttcttt taatgcgttc
     1801 atagcttttg ccacttcggg tgtgattttt gatttcgcat ctagtaatgt tccatcaaga
     1861 tcaaagaaag tgattgcacg ataatttgac acaaataaaa ctcctttttt attggaataa
     1921 gtttatctta acaaagaatg aacaaaagac aaacctttcg tgaagaattc ctgaacatat
     1981 tggttcagtc aacaatttag agtatactat aagaagagaa tttaggaggg aaagaatgat
     2041 ttttttcagt attggtatct tattcttaat aattggcagt ctttttcgga ttctgccctc
     2101 aaaaggaaat ctgccttttt atgggtatca ttcgcctctt gccgcaaaaa cagacgctca
     2161 ttggcggctg gctcaaaaga caagcggaaa ctggtttttt cttatgggct tactgatggc
     2221 attgatcggc tattatttaa agacatctgg gcatacgaat tattttctga ttgaaatgct
     2281 tctgctagta tttccaatta tgcctatttt tattatgaca gaaaagaaat tacagaaata
     2341 tgacctggaa actggaggga acgataatga atattttaat gattgaagat aatcaatctg
     2401 tttctgaaat gatgcagatg tttttcttaa atgaaggttg ggaagcaact ttcaagtatg
     2461 atggaaaaga agggctcgat gcctttttag aaaacccaca acattgggat atgattacac
     2521 tagacctgaa tttgcccaca ctggacggta tgctagtagg aagagagatc cgcaaagttt
     2581 caagtactgt gccgattatc atgctgacag ctagagattc tgaaagcgat caggtgatcg
     2641 gcttagagat gggggcagat gattatgtca caaaaccttt cagtcctctg acgcttattg
     2701 ctcgaatgaa agctttgcat cgccgtagtg agttggcaga agcaagagaa gaagcagtag
     2761 tttcagatga tcattttgat atcgagacta gtcatttcaa gatgaatacg aaaacacgag
     2821 aagcgtatct ggacaaccgg ccaattgaag gactgacgcc taaagagttc gatctattgt
     2881 ttactttagc aaaaaaacca cgtcaagtat tctctcggga gcagttgctt gaattagtct
     2941 gggattatca gtactttggt gatgaacgga cagtggacgc ccatatcaag aaattacgac
     3001 aaaaaatcga aaaggtcgga ccacaagtca tccaaactgt ttggggagtc ggctacaaat
     3061 tcgatgattc tggtgttgcc taatgcgtta tttgtatcag caactcttag ctttctgcgg
     3121 aatggtcgcg ttgatcttat tgatcgttgg agtttctttt acacaactga caaaacaaac
     3181 gattgaagaa aacaattacc agcaactgtt tggatatgct gaatcagttg aaaagacaac
     3241 gcaaacctat gccgatacgc tcccgcagtt tagtcaagat caggcgtttc aaaatgcatt
     3301 gctgctgaca gagcaagtac tgacagaaca aaatgtgaac tttatattta tcgataaaaa
     3361 cgaacgtgtc atttatccga ctaatacagc tttactgaat tttaccttaa cagctgatca
     3421 atggaaaaat ctgaggaatg gacgtcaaga aaaattcaca tcgaataaaa atattttagg
     3481 acaagcacag gcgacctctt acgcacttgt gccatttaat ttgaatcatg aattctatgg
     3541 agcacttgta gttagtcaac ctgcgcgaaa tatcgataac agcgttcgtc caattacact
     3601 gaacctgttc aaaggattta ttttttccag tatcgttgca gttattgcca gctatgcttt
     3661 tgcagcattc caagttaagc gtatcaatcg gttaaggaat gcgacgaaag aagtgacgaa
     3721 tggtaatttt gatgtacagc tccccgtaca tgacaaagat gaatttgatg agttagcaga
     3781 tgatttcaat aaaatgacga attcattgaa agaatctcaa gcagaaattg aagaacaaga
     3841 aaatcgtcga agacaattta tggcagatgc ttctcacgag atgagaacac ctcttacgac
     3901 aatcaacgga cttctggaag ggttagaata caatgcaatc cctgaaaatc agcgagaaaa
     3961 cgcgattaaa ttgatgaaaa atgaaacaga gcgtctgatc cgtttggtga atgaaaactt
     4021 ggattatgaa aaaatccgta cgaatcaaat cagtatggtc attaaaaaat tcaatggaac
     4081 agaaacattg aaaaatattg ttgcacagtt agaagctaaa gcagaagcag caggagatac
     4141 attgacattg aaagccgttg atgatattga tgtttacgct gattatgacc gatttgtcca
     4201 aatcatggtc aatattattc aaaacgcgat acagtttact gaaaacggcc aaatcacgat
     4261 tactttagaa aaaggctatc ttgaaacgat catcacgatt gaagatacgg gaattggaat
     4321 gtctgaacaa caaatgaaaa gtatctggga tcgttattat aaagtagatc cttcaagaaa
     4381 aaatacaaaa tatggtgaat caggattagg tttaccaatc gtgcagcaac ttgtacgctt
     4441 gcataaagga aaattagaag tagaaagcga actaggaaaa ggaacaaaat tcgctgttcg
     4501 tttgcctgac gtagaaatca aaaaagagta aaaaacaagc ggtataaaag atcatgaatc
     4561 gatcttctat accgcttgtt ttttactata aaatgattga tttagcccgc gggctctaaa
     4621 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4681 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4741 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4801 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4861 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4921 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4981 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     5041 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     5101 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa agtgaaaggg
     5161 aatagactaa gaatgagaca gcatccactg agcccaataa gagtttgggc aaatccctta
     5221 agtatatctg tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt ctgttgatcc
     5281 atcggaattg taagtccgct taccgaagtc aagatatcac ggatatctgg cagttcagta
     5341 gtctttaaga caagaaaatt gatcctttga agaagcgctt cataaactga ctggaagctt
     5401 acgatccaca ataaaaccaa gatacaacaa agaaagagag gaaagaacag ctgccagaaa
     5461 ataaaaagga ataatttggt tttagaaaga ccagtcaggt gccaattgat caattcctgc
     5521 ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa tagaaaaaat
     5581 aaactgaata tgccttttat ataaaatcgg ctagctaatt ctaaagccgg aagaagctct
     5641 gtagatgcag aagcgccaat aaatgaaagc cgatcttgga cttgattggc taaagccgat
     5701 tgcaaatggt aaagagtcag gcatgctatc aataaaaaag cagaaaagct tgaaaataaa
     5761 caatagagta ttgtctgctt tttgtaataa attaatcgag cagtagcata gcggattatt
     5821 ttcatacttt tcacctctat aaaaagtata gaaaatgaat atgaataaag tatgaaattc
     5881 ctttagtgaa atcacgaatt aaaaccagaa aatattttgt aaaaagcaag aggttgtgaa
     5941 gaagctgccc tattctatta ccgtagatgc aagcttttga acactgttta ttcgaaaaga
     6001 ggagcagcga caataatttt
//
LOCUS       DAZKOR010000125         5771 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000125 DAZKOR010000000
VERSION     DAZKOR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.128"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..1167
                     /locus_tag="ACG8ZM_002600"
     CDS             13..1167
                     /locus_tag="ACG8ZM_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFQ0493517.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1140..1583
                     /locus_tag="ACG8ZM_002601"
     CDS             1140..1583
                     /locus_tag="ACG8ZM_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493518.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2717..3685
                     /locus_tag="ACG8ZM_002602"
     CDS             2717..3685
                     /locus_tag="ACG8ZM_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0493519.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3695..4183
                     /locus_tag="ACG8ZM_002603"
     CDS             3695..4183
                     /locus_tag="ACG8ZM_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0493520.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4479..4760
                     /locus_tag="ACG8ZM_002604"
     CDS             4479..4760
                     /locus_tag="ACG8ZM_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493521.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4762..5178
                     /locus_tag="ACG8ZM_002605"
     CDS             4762..5178
                     /locus_tag="ACG8ZM_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493522.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         2149 a          984 c          943 g         1695 t
ORIGIN      
        1 taacacaaag gcgtgattat tttgaaaccc aaaaagaaaa tttatctagg tctcctcttt
       61 tccttgatga ttggaatttt tagtatagga attacaaata cctatgcttc agaaaattct
      121 aatttcaata tcaactccca accaattgaa gaacaagcaa ttaattttgc gaaaactgat
      181 tttgaacact ccataacttc aattctttta atggaaaata acaccgaaac tgatattaac
      241 tatactcttg gttcgccatt taaaatgctc gatggagaaa atacggcaaa tagttctatt
      301 ttctttccag tattttcaga cgctgataat acgattaaat atgtttatac cgtcaaaaaa
      361 aatacaaatg gagaatattc aggaagtatt tcacaatttt tagcaaaaga acttcaaaat
      421 ctagtagata gtaatataac agatacaacc ttattttttc aagataatag catttattat
      481 tccgataatg gacaaattaa actactttgg aaatcacctt taagtacaga aaaaaatggt
      541 gtttcttccc ttacagaaaa aaatgatctt atatccgtaa atataaaaaa taaaattaaa
      601 aatttttcta aaaaacaaaa ttttcctgcg gttaaacgtg taaaaagtgt ctcctccgga
      661 gataacttta ttatgattaa ttggcgtata gcagaaaccc aaacaagttt tccgtggtgc
      721 gctgcttacg tagaagctgc tatcttatgt aacaaaacag atgtacgtgc tacttcagct
      781 agagccatta tgcattatac ctatcctaat ttaaacaaca gtcaaattga atcaaaaagt
      841 cttaatcaag atcaaataat aaaatatgca aattactgtg gtgtttatcc taaaaaaata
      901 gcacgaatac taaacctttc agaggtccaa acacaactaa gaaaaggaaa tgccatttat
      961 atgggaactg ctggttatgg aaattatagt aacagccgtc atgcacttgc attatttggg
     1021 tgggtcaatc aaggtggtta tcaaacttac tatgtgtgga atccttggta taactaccca
     1081 actgtaataa gtgcaaatgc aggaccagtt acaattcctg tacctggagg tggttttact
     1141 tgggacaata ccattacaaa ttggtaaata aactcattgc tttagttatg ggtctagtgt
     1201 tagttctttt cttaactagt tgttcaaaaa aacaagaaat acaaaagact gatctgattc
     1261 cttttctagg aacttggcaa actaaagatc attcagaacg tctagtaatc actaatttaa
     1321 atacttcaaa aaatatagaa caatatacca tcttagtaaa agagaacaat cataaaattc
     1381 aaatcattga taaaaattta aatataaatg aacaagtttt catttacaaa gcaacaaata
     1441 atttactaac actgcgctcg aataaaaaac aattagatta cacttttagt ttggattctc
     1501 caaaaaagct cgtatttatt ttgacaacga atgatgggac agaagggaca gctaaaccaa
     1561 ttgaattttt taaagctgat tgatgaatag ttgtttgata ttacaaaaca ataatttcac
     1621 cgaaggtgaa acaagcgaaa ctttacaaaa agtggctagc atttgctagc cttttttgta
     1681 ttgtggagcg tgggttttga gggggattca agggggagct tgcgcccctt tgacaagcgg
     1741 ttatttgcac caaatacgta ggtatttggc tagcaaatat agagcttgcc aagcccttta
     1801 gtgtgttttg tagaaaggga ccttatagtc actttcacat tacaaacgat acgtcctatg
     1861 gacgtatcaa aagggtggga cttatgagca aattaaagca aaatttggct agcaatcctt
     1921 ctaaaaaata cacctattga tcagagtcaa aataaatcag ttttcgagtg agcgaatcca
     1981 aatttgcaac tctgaagctt tgcaaatgag tgtgccggct tccgtaaaag tctaggtaac
     2041 attagtcgat atatacatag ggctctaatt tgcgttttaa ggcgttttaa acgttctggt
     2101 atgtaattat accaaagcga ctttaaaacg ccttaaaacg aattttagag gttctgtaga
     2161 acgttttagg gttgttaggg cttgccctga ccgcctgtaa ggcgctttaa aaacaagata
     2221 agggatttta tctagacttg tcttaaaacg ccgtagatga gcgctcagag cttctaaaag
     2281 gactccttac gaactgtttt taagcaaagg agcctttagg cgactgcgca gtttaatgtg
     2341 agagcgtagc gatcacacct ttaaagcgac cgtagggagc agattcccag tttaggaaat
     2401 ttcgcgaacc tgtttggaga aaaaaagtag gaaaatttta ggcgggaaat tttgcccccc
     2461 tcttttataa tctcttttat aacctctttt taaacctctt taccgtcggc tgtatcctta
     2521 ctctcccaag gtgtgcagaa tcttttaggg gtctttttgt ctgtttttag gggtcttttt
     2581 gtctgttttt aggggtcttt ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt
     2641 ctttaattag ggggttgacc cccattagaa agctctttat aattttgtta agtagttaat
     2701 cttgaaggag gattttatgg caaatgaaat tgtgaaatac caccatgaat taaatacgat
     2761 tcctttacgc aaatttacgt cagtagaaat gaatttattc ttctctataa tatctagaat
     2821 gcgtgatgta ggcgataaaa aagtacaatt tacattcgaa caattaaaag atttaagcaa
     2881 ttataaagca actgcaaatg ttcgattcat agatgattta gaaacaacgt atgacaaatt
     2941 aatggattta cgttttggta gaagaagtgc tgacggtttg caacgtgaac gatttgtttt
     3001 atttaatcaa tttaaaatcg atggaaaagc tgatattcct tttgctgaaa tacaagtcca
     3061 tgagaaagcc ttacccttac ttaataatct tgaagaatgg gttcgatatt ctctccaaca
     3121 attcaatgaa ttagaaagca gttattccaa aaccatgttt agattattaa aacaatacag
     3181 aaccaaagga attgccaatt tttcaaaaga agactttcat gaattacttg atatccctaa
     3241 aagttataga gaaagtgata taaataaaaa aattataaag cctattcgcc aagaactaac
     3301 cgcaatcttc aaaggtttga caatccaaaa gagatacgga aaaggacggg gaaagcctgt
     3361 cattggctat caatttacat ttaaacctga gataaaaaaa tcagacgact ttaacaaaca
     3421 tgctccaacc aaagaagaac aaaataaagc tttggaacta gctaatcaac aggttcaatc
     3481 tgaaaaagaa cctacttcat tacaagaaaa aatcaaagaa caaaaacgaa aattagaact
     3541 tttacaacag ttagcagaat tagaaagaaa acaaaatacc ccaagtaaga aaattaacaa
     3601 acaaatagca gataacaatt ccgatattac cccagaattt aaagaaaact tactagataa
     3661 tctccaaaac ttatttaaag aataaggggt ttttatgaca gataaaacta tcaaagaatt
     3721 agcagatatt ctaggtgttt caaaagataa agtgaagtat caactgagga aattacctag
     3781 taattttacc tacaaaaaag gaaatattac ctacctgaaa aaagaagcag taacaacaat
     3841 tacaaaggtt ttatcaggta atgaatcggg taacttacct agtgaattac ccgattcatt
     3901 acctaccgat tacctactca aggagattga aactaagaat aatcatattg aagaaaaaga
     3961 taggcaaatt gaaaaacttc aaatagcttt agaaacgcag caaaaattac tcgaccagca
     4021 acaacaactc accctacaag ccaatcaaca aatcaaaaaa cttcacgaac aactccagct
     4081 aacctatgca gaagaatctt cagaaaaaga acccctttct gttactgaag aaactcaaaa
     4141 aggacaaaac aaggagaaaa aatggtggca attctggcaa taacccaaca atttttattc
     4201 ataaaaattg ttgggttact ttcttttttt gcttttacaa gcatagcgac atgatctcac
     4261 gtcgctattt tttaaagagc ataaggaaac gcggtatcac tttcttacga aaggtgctag
     4321 gtaggctgtg cctaccacgt ttctaagaaa aaaatttgct atcacttttt cgtcattgtg
     4381 gatagcttat gtatagtgtt tcggcaaaaa acagctatac aaatactata tttgtatgat
     4441 atacataaat tatacacaaa tgcgaaagga attcttctat ggacatttta gttcggaata
     4501 ttgatccaaa agttatttca gaaatagata gacgttgcca agaactcaca acaaaaactg
     4561 gcaaaaaatg gagccgtaat gcgtacctaa aagttctaat cgaaaacgat tttgaccagg
     4621 cacttaggca ttacaaaaaa gatcaattcg atttattact cgaaaaattt atcgatatac
     4681 aagcaaaaaa tactgaaaca ttgaacgact atgttcatac aaacaaccaa ctcattgaaa
     4741 cacttatcgg aggaatataa catgagaaaa acttctgttc aaaaacacgt taccctttat
     4801 cccagtcaca ttgacgtttt agattccatc atcgaaaaaa atccagatat caataactac
     4861 tcggaagcca ttcgttttct atgtatgagt tatgaaccta atactaatac gacaactacc
     4921 agcaaccaag caaaactaaa tgcgatggga aaagaaattt ctattatgtc cgaaatcatt
     4981 gcagaatttg ctgatttata tctaaaagat actggtgttc ttatcggtac aaattctaaa
     5041 gtatatcaag aagtgaaaga aaaaattgaa aatcaactca atccctcccg acgaaatcaa
     5101 cccaaaaatc gtcaaaaaga aaccaatcaa acattcactt ctagaaattt tgacccaaca
     5161 aactttctaa aaagatgaaa aagaaaccga aaattttttc tatttctcag tttctttttt
     5221 agggggcagt cttccagcaa gatttgcaag ttaccggtaa cttgcacaaa aaaagaaaaa
     5281 tccgcttgtg cgaatccttc tttttgcatt ttggggcgct gccccaatct ccaaatatcg
     5341 gttatttaat agcgtggtaa ctcaaaaaaa tagaacgtac tctgaaataa aaaaagactg
     5401 ctctaacgag cagccaataa aaaataatga gcgtttattc ttgaaaaagc agcgcgctta
     5461 tgaagcgctg ccaaaaattt accatatttt atgaaaaata acaagtttct aaaaaggaat
     5521 cggttaagat tcacaaagaa tcgtaaacag acaatagcgg aaaatcaagc ttccgaacgt
     5581 accttttagc tagaccaatg aaaaatcaca aagtgagccc ctgtatcggt tgatacaagg
     5641 gctacttggc atactcgaca ttatgtctac tagctcgata aataacgatt atgttcccaa
     5701 aacaatattt tatgaactat aacaaaaatt tgttatcgca tattgtatat tttaattata
     5761 ggtgtatatc a
//
LOCUS       DAZKOR010000126         5339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000126 DAZKOR010000000
VERSION     DAZKOR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.129"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..414)
                     /locus_tag="ACG8ZM_002606"
     CDS             complement(19..414)
                     /locus_tag="ACG8ZM_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493523.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(404..616)
                     /locus_tag="ACG8ZM_002607"
     CDS             complement(404..616)
                     /locus_tag="ACG8ZM_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0493524.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1688..2290
                     /locus_tag="ACG8ZM_002608"
     CDS             1688..2290
                     /locus_tag="ACG8ZM_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFQ0493525.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2645..2881
                     /locus_tag="ACG8ZM_002609"
     CDS             2645..2881
                     /locus_tag="ACG8ZM_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFQ0493526.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            2878..3291
                     /locus_tag="ACG8ZM_002610"
     CDS             2878..3291
                     /locus_tag="ACG8ZM_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0493527.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            3834..4760
                     /locus_tag="ACG8ZM_002611"
     CDS             3834..4760
                     /locus_tag="ACG8ZM_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFQ0493528.1"
                     /translation="MTGRNPKVFISYSWENKENKEWARELADKLLENGIDAYIDQYDL
                     SLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAELLSGKNERK
                     FIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDIPRH
                     IKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLWKEL
                     FVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQEKK
                     ILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4757..>5339)
                     /locus_tag="ACG8ZM_002612"
     CDS             complement(4757..>5339)
                     /locus_tag="ACG8ZM_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0493529.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1908 a          760 c          902 g         1769 t
ORIGIN      
        1 ttgtatgaac ctttatttct acattttttt ctcaagcaca ctactgataa tgattaaaat
       61 aatcggctgg attgcaatga tacttccgac aataatagct ggaatgatgt agtctaaaca
      121 aataaatata actgttgcca cccctaacag tattgtgtta aacatatttg ttatatttcc
      181 cgctttttct ttgattatga tattccgttc gtccataaca ttgattttgt agtctgcatc
      241 atttttaatc attttatcat gcaaaatttg aacatgagaa acgccaaaaa cggcactgaa
      301 taaaatagca agtccaatat ctaccatttt aggaaaatct gtaaccaaaa ttgtcacaat
      361 caaaaacaac gaaacaatat aaccgatata ccaaatctga cccttatttt tcatcttcac
      421 tttcctcctc atagataaaa atttcttcaa tgctcatttg aaagtacctt gcaattttaa
      481 atgccaatat aatagaggga ttataacgtc cattttctaa agaacctatt gtttgacgag
      541 atacttcaag agcagaagct aaatcttctt gtttaattcc tctttgtttt cgtaattctt
      601 ccaaacgatt tttcacaaca tgctcctttc taaaaaatgg aaagtttact ttcctttcgt
      661 gcttttataa tactcctgcg agcacaaaat gtcaaggaag ctttccatat aaattttgat
      721 tttaatgttt tagttactgt ttttatgaca catgaacaca cttattctta tttcctctta
      781 tctgctatca acagaaaaga aaaagccctg tcaagagccg attagatcaa gccagtcgag
      841 aaacgatgtt agaccaaatt gattttgggt aatgaatgtt tagaacttag tagtcatcca
      901 aaaacgacta ctttttttgt tgaatcgtcc aaggacgatt cgatgctttt gacttgtaag
      961 aacaggattg aatgaaacca tactgttcgc atatttttag tggatgaaca aacaaaatac
     1021 gagagatttt ttgttcgttc atccatggtt ttagaaaaaa gagggacgat tttggaagaa
     1081 gaaaatcgtc tctttttttt cttctttttg tatgacaaaa agaaagatct tttgcccatt
     1141 ttatttttat aaaatgggta ggtggcgttc gcgtaaagca aatcgataca atccaatggt
     1201 tttggcgaat cagatttacc attctaggct tgtttaatac agacatcgat tgttattttg
     1261 agaatttata tccttattgg caatgaaatg gatattctcc ataccaatag aaatcttcaa
     1321 gataattttc ggaaatatat aagagttaat caattatgat catatagtag gtaaaaatta
     1381 ggacagcctt caattatatg ggaaaccaat ataatcaagt aattattcgg gtaacacaaa
     1441 tactagagta ctcatttctt ataactgttt tacacctatt agggatcagt attattaata
     1501 gatctagata aattaatacg taattatata ataatttatg tttgtgccat taaacaatct
     1561 taacagatat ttcattttat ttatgtaaaa caacacgaat aattattgct tcgtaaagtt
     1621 atatgagtat aagttaattt actggaggta gtgcaatgct tttttctgaa agatttggtt
     1681 ttaaaaaatg gaggatatca tactgagaga tagattgaca gaccctctta aaaatagttt
     1741 agcaaatgtg atttatgata tattacgaga ggctgataaa gtttatagta attataatgg
     1801 aaaagtctca tgggtaatat attctttttt aattaaagag ttttttaaag aacagataaa
     1861 taatagctat gagttgggtt atcattttac tgtttattat gaagatatta tcaagaggtt
     1921 agaagacttg gagtggtatg cttattatga tttattagag gtttttgcaa gtgctaaaat
     1981 cttggataaa aagaaaagag aggaagtaaa taaaattctg gagtcggaaa attctggtta
     2041 cagattaagc gaaagcaatg aatttattcc aatcacagat gatatttcta ttaagtctat
     2101 aaatgctgta tctgaatcac catttaatta cgcaaaaaat catatccata aatctcttac
     2161 atatataagc gagaaggaaa atcaagatta taatactgta gtgagagaag caattagtgc
     2221 tgtcgaaagt tgtcttatcg aattttcagg attaccagct aataaaaaaa tactttggga
     2281 actgctataa aagcaattaa agacaaatcg aataacaatg tcgatcttac atttttaaaa
     2341 ccatttgaga caatgtatgg tttggcgagt aataatggaa tacgacatgc agaaaatgaa
     2401 aaaacaattg tctcagattt acctgatgca gttttggtgt taacaacatg ttcagccgta
     2461 attaattatt tgggagtaaa aatagcatat gactccaata aatcatgaaa agttaaaatt
     2521 catccaagag ctacattata actattaatt tccaactctt taaaagttta gtaattgaag
     2581 gaagtaataa gggtgattga acaatacgga tatacggtgt atacttatcg taggagttga
     2641 tgatatgaat attaaaaaac gcaaaatcag agaagtaggt aattcaatag ttgtaacttt
     2701 gtcaaaagag agtttattgc aaaaaggttt gaaacctgga gatactattt ttattgatga
     2761 agataaaatg atggaagcta ttgtaaaaga ggaaagcaag atagatcttg aaattgatat
     2821 gtatgttaac caagctttat ctgagtatga tgcagctttc aaggaattag ttgaaaaatg
     2881 atctatctaa gtgaaaaaga tattgtaaaa ttgaatgtta ttcaaataaa gaaatattca
     2941 ccgcgagaaa caatcggagt cattgacaaa aatgcgctga gtatggctgt aaatcaacct
     3001 aaacagcata tttttggaaa agatctgtat cctgataaat ttagcaaagc agctgttctg
     3061 ctaataaatc tgatcaaaaa gcatccattt cataatgcta acaaacgtac agcattttta
     3121 gccgcgtatg tttttctcaa gctaaatggc tattcactaa aaatgaaaaa tcaggaagta
     3181 gtagaatttg tagttgaaat tgctacgtat cagggtgatt ttgatgactt aaaagaggca
     3241 acaacagata tactaaaaaa gaattcaaca aagaccgatt caaaaaagta gaagcaaact
     3301 agggattcag ttaaatctga agatttagtt tgttctttat tttagtttac ataagataca
     3361 ttatgcgaag ttcattattt taaaagctta aataataaac ttttttataa taccaattta
     3421 tttatgtaaa ccaatgacct taatcgctta agcaagcatg ttatgtaata ctctactctc
     3481 tccaaaacta atgactatct attttcaaag aagctagaat gattatctga tcaaatgata
     3541 atttatattt aattcctggt ctactgctct tcttttaatc aattaatgtc taacgtactg
     3601 tacacgataa agtaggaaga cttattttca acttcggatt cattttttta attcggtgaa
     3661 aatcactaaa ttaagagttt atccaagttc attttagctg agaatacgct tgaaaataat
     3721 tttgatgaat tttcctgaat taaaatttat taacttgttg agtgatataa ttgtaatgta
     3781 ttaccaaata gttagcactt agattagtga tggaaaggaa tagtgagaac aagatgactg
     3841 gtagaaatcc caaagtattt atatcatatt catgggagaa taaggaaaat aaagaatggg
     3901 ctagagaact agcagacaaa ttacttgaaa acggtattga tgcatatata gatcagtatg
     3961 atttaagttt aggtgataga ttacctcagt ttatggagca gcaaatatcc gattcagatt
     4021 atgttctaat tatatgtaca ccagagtata aaaaaaaggc aaataatcgt acaagtggtg
     4081 ttgggtatga aggtcatatt atctcagcag aattgctttc tggaaaaaat gaacgtaaat
     4141 ttatcccaat aattaaagaa ggacgtgcaa gcgaagtact tcctactttt cttgctggaa
     4201 agcttggaat tgatttatca gatagccagc attatgatga aaacttagat gatttaatta
     4261 caactatatt tggtgttaag agaaaaccag cattaggtga tatacctagg catattaaag
     4321 gcagtccttt actacctata gaacataatg aaaataaaga tattgaaatc ttaggaatta
     4381 tcactgataa agtaactgta cctaaaatgg atggctcaag gggttctgca ttatataaaa
     4441 ttccatttag attaaataga gtaccaagtc agctttggaa agagttgttt gttagaaatt
     4501 ggaattcacc accacgatat acaacaatgc atagaccagg aattgcttct gttgtagacg
     4561 atgaaatttt acttaatgga acaactattg aggaagtccg ggaatatcat cgggaaactt
     4621 taaaactttg cattgaaaaa agtaatactc aagaaaaaaa aatattgcta gaggaggaac
     4681 aaaaaaaaga gagagagaat gagcgagtca gatcacataa agaaagtgtt cagaaattag
     4741 cagatgaatt agagttctag ttgatagtca gttctttctt aattcgatac acagtttgtc
     4801 tagttacatt atagtctgtt gctatttttg aaatagcaac accattctcc tggtcctgaa
     4861 cgatgcttct ataaacaagg cgacgttgag gatcttttgt gttcgcactg tataacttag
     4921 gacgtccttt atagacgccg tttgctttag caatctttat cccttgtgcc tggcgtcgtt
     4981 ttgattcagt acgctcttgt tcagcaatca tggccaagat ttgaatgatt aggtctttga
     5041 taaatttatc caggagagga tttccgattg cttcagccat gatcggtaaa ttagtgataa
     5101 taagtcgcac atttttcttc tttagataat ttactgaatc gataatctcg tcataattac
     5161 gtccaagtcg atcgatagcc tctacaataa aaatatcttg gtctctaaca aaatctagaa
     5221 cttcctgaaa aatcggtcga tgagtaatag tcgctccaga ttgtttctcg atgaaaattt
     5281 tttcagcacc aaattttctc atttcctcga tttgacgttg ttcattttga tcgatcgat
//
LOCUS       DAZKOR010000127         5309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000127 DAZKOR010000000
VERSION     DAZKOR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.130"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..1219)
                     /gene="nagA"
                     /locus_tag="ACG8ZM_002613"
     CDS             complement(71..1219)
                     /gene="nagA"
                     /locus_tag="ACG8ZM_002613"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0493530.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1295..2131)
                     /locus_tag="ACG8ZM_002614"
     CDS             complement(1295..2131)
                     /locus_tag="ACG8ZM_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493531.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2315..3532)
                     /locus_tag="ACG8ZM_002615"
     CDS             complement(2315..3532)
                     /locus_tag="ACG8ZM_002615"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0493532.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3681..3842)
                     /locus_tag="ACG8ZM_002616"
     CDS             complement(3681..3842)
                     /locus_tag="ACG8ZM_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493533.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3915..3998
                     /locus_tag="ACG8ZM_002617"
     tRNA            3915..3998
                     /locus_tag="ACG8ZM_002617"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3949..3951,aa:Leu,seq:gag)
     gene            complement(4238..5309)
                     /locus_tag="ACG8ZM_002618"
                     /pseudo
     CDS             complement(4238..5309)
                     /locus_tag="ACG8ZM_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1653 a         1082 c          838 g         1736 t
ORIGIN      
        1 ttactagaac acaaaaaaaa ccttaaaacc aaacttaaga agtttagttt taaggttaac
       61 ttaaattata ttatgcgtgg aaacgttcca cgccatctag ataagttgct tccaatgtca
      121 tatcagggtt caatacgata aagtctgcat cgcgtccttg agcgattttt ccacatgtat
      181 catcgatttt acagctgatt gctggaatca gactagccat catgactgct tgttctggtg
      241 ttgctaatcc ccagtctaca acatttttaa ttgcttcttt caatttcaat atacttccag
      301 ctaagttgcc agattctaga cgtgctgttc cttcttttac tactactgga aattcgccta
      361 aattgtaatt tccatcaggc atcccaccgg ccatcataca gtctgtaatt aatgcgacat
      421 gatcatgtcc tgctttttcc attaggattc ccgcagcatt tggatgtaca tgatggccat
      481 cacaaatcaa ttcagaaaag acatgttcta aagataataa cgcacccacc atacctggtt
      541 cgcgatgatt caatccacgc ataccattga aagcatggac aaatacactt gcgcctgctt
      601 caacagcttt ttgcgcttct tctagtgtag cattactgtg tcctagagaa acaactactc
      661 cttcttcagt cacttgtttg acaaactctt ctacaccttg acgttccggt gctaaagcaa
      721 tttttttgat taaaccacct gaagcttctt gccattcatg gaaagtatcc aaatcaggat
      781 ccccgaaata acttgggttt tgtgcccctt tgtgttcttc tgtgaagaaa ggaccttcaa
      841 aatagattcc tttgatttta gctcctggtg cttcctgata cattttgccg attgtctcag
      901 ctacatcttt taaacgttct ttgcttgacg tcaatgttgt tggtaaaaat gaagtcactc
      961 cacaagataa cagaccttca gacatcactt tgatgccttc tgcatcatta tccattacat
     1021 catgattcat atagccgtga atatgtgtat ctaccaaacc aggagcaatc catttgccgc
     1081 tttggtcaat gatgttgact tctccctcag gtagttcttt ggtgtattca ccaaaaagtc
     1141 catcggtaat ttcaagatag cctgcatgtt tgacacctga cgctaaaaag aatttgtcag
     1201 catagataaa agttctcatt ctattctctc cctttgtttt tctacctcta aagtactctt
     1261 atttagagaa gaagtcaaga atggtctaca ccatttacaa attttttgaa atttgtttca
     1321 cagcttgtgc taagccttca tagaatgctg ctctttctgg ttcttcttta cgcttgtata
     1381 ttaagctttt aagcacaaat aaatcattga ggtaatagta gctattcttc tcttgagccc
     1441 aatagattcc ttgattcacc tgttctaacg cttcgtcgat ttcattttga tggaataaga
     1501 aatttccata attataaaaa atttgcgaaa attcactttt tgatcgttca tcagctgatt
     1561 gatgcagcag ttggatgctt cgactcaaat agtagcgtgc ttcagccgtt tttcctgcca
     1621 caccatatat ttttccgata caactaatca gttgtatttc tgtgtcagaa acatggattc
     1681 tttctttttg gctagagtag cttaatgctt ctttcaaata ataaagggac tgagagatat
     1741 tttgacgcaa cgtgaattcg caaacaccga gataataata atagcgttga aagtcagttg
     1801 cttgatacag attttcagaa atatcagaag attttagtac ttgttctaac ttccgataat
     1861 cacgtttttg gaaacaacta tccaacagtt caaataattt atcgtttgtc cgagtagttt
     1921 ttcgagagag attcatcaca tgatcaatcg ttacccctag ccgttgacaa atctgcatca
     1981 tcaccaatac attcggcagt tcctcattat tttcgatccg acttaataca ctttgtgaac
     2041 aaatatcatg cgaaagcatt ttttgcgtca ttttttgttc ttttcgaatc tctttaatga
     2101 tcgttccaaa ggactcgatt aacgtttcca taagtatcct ccccaaaata tgcgtattcg
     2161 cattctattc gtattttact ttaggaagtc gcaaacttca agaaaagact tgcaactaaa
     2221 taatatactt tagttattta agaaaactcc actagaaaga gagcctcagt gtgtttgtct
     2281 gtggccctcc tactgaaaaa tttggttttt aaaatcattt ttggcgatag gttgctagga
     2341 attctctcat tttcttagct gtttgtttca aatcatccat tttaggaaga taaacgattc
     2401 gaaaatgatc tggctggttc caattaaatc caccaccatg tacaagtaga atatgatgtt
     2461 catgtaaaaa gtccaaaacg aatttttcat catccaaaat attgaatcgc tttgtatcaa
     2521 tttttgggaa tatataaaaa gcagcttttg gttttacggc tgaaagacca ggaatatcat
     2581 tgattgcatt atagatatat tctctttgtt catagatcct tccgcctggc aggagcagct
     2641 catcgacact ttgataaccg cctagcgcgg tttggataat ttgctgagac aatacattgg
     2701 aacacaaacg catggaggac aacatattca gcccttctat atagcctttg acgtgtgact
     2761 tatctccact taggaccatc cagcctactc gaaatcctgc tacacgatga gatttggata
     2821 gaccgttcag cgtaacaacg aacaaatcag gcgctaatgt agcaatcgga atatgttcta
     2881 gaccatccat cactaagcga tcatagattt cgtcggaata aataatcagg ttattttgtc
     2941 gagctacttc aacgatttct tctaataatt cttttggata aagtgcccca gtaggattat
     3001 taggattaat gataacaata gcttttgtcc gactcgttat ttttgatttg atatcatcaa
     3061 tatcaggata ccattctgcc tcttcatcgc aaatataatg aacaggtgtg cctccagcta
     3121 atgcaataga agccgtccac aacggatagt ccggcattgg aaccagtact tcatctccat
     3181 tgtccagcaa cccctgcata cacatcgtga tcaattcgct cactccgttg ccggtgtaga
     3241 tatcattgat cgtcacattc ggaaaatgtt tcagttggca atattgctca attgcttttc
     3301 tagcagaaaa gatccccttg gaatctgagt agccttctga gtcacgggca ttcatgatca
     3361 aatcacgaat gacttcgttt ggtgcttcaa atccaaaagg tgcgggattt cccgtgttca
     3421 attttaaaat actaatgccc tcttcttgca tccgctccgc ttcttctaga acgggaccgc
     3481 gaacatcgta actgacacct tctaatttgt tggatttttc aaattctctc atcacttagc
     3541 caatccctca tttcaaaaag ttagaacttt ccaaattttt tgttatttac tatttcaata
     3601 tcatactcca tcctagaaaa ttttgccact tatttaggaa aaacgtaagt attaaaaaaa
     3661 cacaaacaag cgttgagaac ttaattgtca tgatagtcat tccatagatt cgctgcccaa
     3721 tccattgcaa ttccacaaat gattcctagc ataaaaccaa gcaaaaaacg tccgaatgca
     3781 agtccaatca atacacctaa gatcattccc agtcctgaaa attctccgtc atatttctcc
     3841 atcttaataa gtcctcccat ttttgagctt gacaactatg taaaacctcg ctatcattag
     3901 tgtgttatta ttacgcagat gtgttggaat tggcagacaa gcatgattga gggtcatgtg
     3961 agcgttagct cgtgtgggtt cgagtcccat catctgcact acaactcgtg aagttaattt
     4021 tgcttcacga gtttttttgt ttgaacgttt ctttatgcca ttatatacat atcactgtat
     4081 cctttacaaa aagaacgtac aatcatgtaa acagcctgag gtgagtatat gaaggtcgga
     4141 aacaagctac tcgtagaaac ttgcaagtgt taaaacaatt aatcgaaaaa cgagtaaaat
     4201 ccctcaccta atactaaagc gagggatttt ttgtatatta tctgtttatc accgttatta
     4261 taaaggtaca aataccaagc acggttaatc caaattgcat aaattcattt ttttgtacac
     4321 tcatgctttt cattagctct ttaactgcca ttagtactaa gatggcgata acaacataaa
     4381 gtatatagta cgtccgcatt ccggaagccc atactgtggg agaaaatccc ataattaacc
     4441 ttgaacaaaa tcccattatc atcaaaataa ctaaaaaaat agaagttttc ttattgtcaa
     4501 aacttaaata aattccaaca attaaacata gtaataatgc caagactact aaagtagcta
     4561 tccaagtacc tggataaaaa agtgacagtt tcgttcctaa ctctgtaaag agattattca
     4621 ggttactact actccaaatc attgcccttt tatttccatt gacgtaagta aagctttgac
     4681 ccattgtatt accaaaataa ataatcaggt ttaagaaaaa cggtaatgca gtcaatatac
     4741 gcacataata gttttgacat ttcctatatg ttagaaggaa aattaagaaa aatagcaata
     4801 aaaataagat attcatgtct aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa
     4861 cttattaaga aagttgaaat ttttatattc aggaaaccat ttattaattt catgtgcagc
     4921 acgtaatgca ttccctggag ttgtcaaaga gaaaattaat tctgctaagc ttattattga
     4981 aaatactgat aacttataat tatatcgtcc attgacaatc aaatataaac taactatact
     5041 tgttagaaca aaaaaacagg cgttgacttg ttcttgatta gttaaaaata tcaataaagg
     5101 aatacttacc caatatattt ttctatttat ttcttctcct cttaagagtt gaaaaaacgg
     5161 ataaaaagtt agtaaactaa atgctactgg ccactgatag ttcaaggtgg ttgctctcca
     5221 accggtttcc cccattactg tcaatggtat taaacagtat atagagcaaa ccaataataa
     5281 atttttaaca tttattttct caaaaacat
//
LOCUS       DAZKOR010000128         5207 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000128 DAZKOR010000000
VERSION     DAZKOR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.132"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1368
                     /locus_tag="ACG8ZM_002619"
     CDS             <1..1368
                     /locus_tag="ACG8ZM_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493534.1"
                     /translation="SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIAL
                     TIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSK
                     QIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDS
                     IIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFL
                     FQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIA
                     AKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGE
                     SWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVD
                     VNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINK
                     KNENK"
     gene            1355..2989
                     /locus_tag="ACG8ZM_002620"
     CDS             1355..2989
                     /locus_tag="ACG8ZM_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0493535.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3164..3997
                     /gene="msrA"
                     /locus_tag="ACG8ZM_002621"
     CDS             3164..3997
                     /gene="msrA"
                     /locus_tag="ACG8ZM_002621"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0493536.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4077..5066
                     /locus_tag="ACG8ZM_002622"
     CDS             4077..5066
                     /locus_tag="ACG8ZM_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0493537.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1825 a          806 c          951 g         1625 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttggtctgtt
     2221 atctttatgt attcccctga ttttatttaa aaaattaaca aacatagaaa aaaagattta
     2281 ttatggtacg tgtttgactt ggctagctac aactagtttg gtgccatggt atcttctgca
     2341 aaatacgcca gccaaattgt tacaatttcc ttggcggatc ttaagcttgc aaataatatt
     2401 ctcttctctg atattaacga tgatattttt caaaaataga agatacaata agacaagaga
     2461 actattttat ttatttatga ctgttttgtt attaatcact ttaactgttt ctagtaaaaa
     2521 aaatttcaat caaaaaataa tttctcagcc gggacatatt gttgtaaaca aagaaacagt
     2581 agaggcgtat accactaaag gaatggatta ttttttatat gattattctc ctatcgcttc
     2641 attggagaat aaggagcata taataaaaca cgaagcaaag attggcagtc aatgggtaaa
     2701 ccttccttat aaaacaactg gtagttctat catatataga gtaactaatg agaagagcca
     2761 agtaatcaca cttcctgtat atgcttattt aggaacgaat gtagaaataa atcatcaacc
     2821 tgtaaaggag caggtgaatg ctaatggatt ggtacaaata agcggagtca aaggagttgc
     2881 tgatattaaa gtaagcacaa gttatacagt aacgactatt atttcttatc ttgttacatt
     2941 aactgcatat ttatgtttat tttttaaaat agttttccat aaaaggtaaa ttaacttttc
     3001 atttataaaa taaattgatt aaaaaggaga atctgtcgat aaggattctc tcttttttat
     3061 acgaaaaatc tgtaggggga tgaagtaatc gcttatttcc tcactttatg ataaattaca
     3121 gctaaagtca ctagcttgtc ttcttcggaa aggaggagaa tctatggatg aggaacaaat
     3181 attcattcaa acactttata atctgatttt gaatccgaat acacgagatt gggaacgaaa
     3241 agtattgatt cagacaaaga atgacataag agaaaatata tccgttaaag aacagttatc
     3301 aaaactagaa gcaacactac gtccattagc tatccgaatg aatctgacgc ctgatgtaat
     3361 ggacttttat ttattgatta cggaaggatt tgataaggag caaaaatatg acttttccaa
     3421 gcatgccatg caagatgctg actatcaaga acgtgcagta tttgcagggg gatgtttctg
     3481 gtgtatggtc gagccgtttg aatcgaaaaa agggatccta tctgttttgt caggatacac
     3541 gggcggacat gttgaaaaac caaattatga tcaagtaagt ggtggttaca caggtcatgt
     3601 ggaagctgtg gagatcattt atgacacaag agaaataagt tatagcgagt tgctgacaat
     3661 ctattggcaa attactgatc ccacagatac atttggacaa tttcaagata gagggaagca
     3721 atatcgtcca gtgatttttt atcaaggcga acgacaaaaa gaattagcgg aacaatcaaa
     3781 acagaaacta gacagttcag gaactttcca ccaaccaatc gtcacaaaaa tcgaaccagc
     3841 cggtacgttt tggccagcag aaaactatca tcagcaattt tacaaaaaac aaccgaaacg
     3901 ttataaaaaa atccagcaag cacgtaatca atttctgatc tatcaacgag taaaaaacaa
     3961 atggcaaaaa aatatacgaa aaaatcattt tgattaactc cttttcttta aaatgtagta
     4021 aactaaaaat agaaaaaaga aagcgtttta aaaagatttg atgaggagga gaaaaattgt
     4081 atattgcaga tagtgcacgt tatgaaaaaa tggaatatcg taaatccggt cattcaggtt
     4141 tgaaattacc gattttatct ctcggattat ggcaaaattt tggtgattat gatcctatcc
     4201 ataatcaaag agagatttta agaggcgcat tcgacatggg aattacccat ttcgaccttg
     4261 cgaataatta tggaggccct gctggagctg cagagaaaaa ctttgggcgt atttttagag
     4321 aagattttca agcctaccgt gatgaactga ttatttctag caaagcaggt tatcatatgt
     4381 gggaagggcc atatggggaa tggggctctc gtaaatccat catttcaagt tgtgaccaaa
     4441 gtctccaaag aatggggcta gattatgttg acatcttcta tcatcatcgc ccagaccctg
     4501 atacaccttt ggaagaaaca gcagaagctt tgatgcaact tgtaagacaa ggaaaagcat
     4561 tatatattgg tatatccaac tataatggag aagatacaaa aaaaatgact gaaattttaa
     4621 aaagaaagga agcaccgttc attattcatc agatgcgcta caatatgttt tctcgtgcac
     4681 tactagaaga cgatctatct ccagtattag aagaagaagg gctaggggca atcacgttca
     4741 gtccgcttgc tcaagggttg ctgacaaatc gttatctaca tggtattcct gaagattcta
     4801 gagcacatcg taaggaaata ccattccttt cagaagaaca agttggatca actctggaaa
     4861 aaatcaaagc actgcaaaca atcgccgtat cacgaggaca atcattagcg caaatggccc
     4921 ttgcatggaa tttgcgacaa aaatctgtta caagtgtact ggtcggagca agtcggttaa
     4981 gtcagttaca agaaagtgtt cgaatgatgg ataatttaga tttctcacca gaagaagaat
     5041 tgagaatcga ccaaattcta gaatagaaac gatgtaatca aactttttat ccatatatta
     5101 aaaaaaacaa aagatgacat caagaatcga atgaagatcg tctgttattg aacatcatat
     5161 tttttgtcgt tcaaaataaa aatgatcgaa ggctaggagt gaatttt
//
LOCUS       DAZKOR010000129         5179 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000129 DAZKOR010000000
VERSION     DAZKOR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.133"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..1841)
                     /locus_tag="ACG8ZM_002623"
     CDS             complement(75..1841)
                     /locus_tag="ACG8ZM_002623"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0493538.1"
                     /translation="MQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINMVP
                     AFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLRMQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1801..3555)
                     /locus_tag="ACG8ZM_002624"
     CDS             complement(1801..3555)
                     /locus_tag="ACG8ZM_002624"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0493539.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3665..4234)
                     /locus_tag="ACG8ZM_002625"
     CDS             complement(3665..4234)
                     /locus_tag="ACG8ZM_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0493540.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4252..>5179)
                     /locus_tag="ACG8ZM_002626"
     CDS             complement(4252..>5179)
                     /locus_tag="ACG8ZM_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0493541.1"
                     /translation="ILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKEL
                     IDQVLFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITY
                     ENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLV
                     MQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLL
                     VSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNR
                     IIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1727 a          969 c          694 g         1789 t
ORIGIN      
        1 aataaaatta ttctttttga aaaagaatta taactatatc ccaaaaattg taggttaatt
       61 taaagcctat ttcatcatcg tgattgagct tctttttcat agacgaattt ctcagatagt
      121 ttagttttct ttatgagatt tttgtatatt tctgaccttc gatacaactc actatgagta
      181 ccttgatcaa ctatttttcc ttgatccatc actacaattt tatttacctt ttcaatagac
      241 ttcaagcgat gcgatataat tagtactgtc ttatcttgta ttaaacgatt taaactttcc
      301 tgaatacgtt tttcattttc cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt
      361 ggcgcatttt tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt
      421 gaacttccgt tttctccgac caatgtatga taaccgtcag gtagtttttc aataaagtct
      481 tcgcagttag ctaaacgagc tgcttttttc acttcctcat ctgacgcttg actatttcca
      541 atacgtatat tttccaaaat acttgtatta aacaaagtta cctcttgaaa aacaatagcg
      601 attttgctaa acaaagaagc tggagataca cgtttaatat catacccatc aattaaaata
      661 cagccttcat cataatcata cagtctagac actaatttca agatactagt tttcccggaa
      721 ccactagcac ctactaacgc agtcacttct ccttgtttag ccgtaaaaga gatatgatct
      781 aaaattgggg tattgttatc atatgagaaa gagacatctc gcaactcaat gtcaaaagat
      841 ttcagaggac tatctgatcc ctcttggata ctggtttcct tcatagctct aattcgctgt
      901 attttaggag ctaaataaaa tatttcaagc actgcttctt tcatagaatc gaaggaatcc
      961 tttattttta ttgcggctag taagtaacca acgacgtaga gtatagtcac ttcacccgtt
     1021 aacagtaagt gaacacctac tagaatgact actgctaaac taacatatga gaaaatagag
     1081 gataaggcca tgactgaaaa agtacttaat tctaccttta aatgaatcct ttcactctct
     1141 tccatttttt tgaaaagtct gtcttgaact tttttagata gattaaagct attaatctct
     1201 tgctgcaatt ctatagtttc ttgaaattct tccgagtttt ctctcaaagt atcgtaatat
     1261 ttagtattgg ctttcgtttg ggatttcttt gataacaaga ttaagacaaa actaaataac
     1321 gttggcaaaa taacagctaa tcccattttg acattaccaa ccaaaagcat cactgaaata
     1381 aaagggaaaa acagagccat accgccggct ttaggtattg catgactcat cgcatgctca
     1441 ataccttcaa cgtcagacat gatagtttga gataaatctg ataaattatg ttttgaaaaa
     1501 taggataatg gtaaatttga caaatcgtct gcaatttgca ttcttaaatg cgcactttct
     1561 ttataagtac tattatataa gttttcatac tccctatcta acaaccaata catgaaaagc
     1621 aaagtaacga acgatactgt aaaataaagc caaggcggct ttacattttc taaaactaat
     1681 tcgtctatca gcatcattat aatgaatgct ggtaccatat taatgcagta tacaaaaaag
     1741 ctagaaatag atgctttaac caaatcttct gcgccctgat tagttaaagc gaatttgttt
     1801 ttataaaaac ttttcattgt caagcctcca atcatttgca ttctcataat tagtgaccaa
     1861 ttgtttatac acttgatgtt tagccattaa ttcattatga tttccacgtt ctactatttt
     1921 cccttctgac aagacaagaa tttcatctac agatttaatg ctggataatc gatgagctat
     1981 cataataact gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt catactcatt
     2041 atctggatca atagatgcac tggcttcatc cataataaca atctttgcat cttttaaaat
     2101 tgctctggca atagctatac gttgtttttc tcctcctgac agataaactc ctttactacc
     2161 aattaaggta tgctctcttt cgggaaattt ttctaaaatc gattcgcaac cggctaatct
     2221 tagcgcgtca aatactttac ttgctggcgc ttgaggattt gctatagaaa cgttatcgta
     2281 aatactctga ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt taataattgt
     2341 gtcttttgaa tagtgattta aaggcttacc accaatttta atctgtccct tatccacatt
     2401 gtagaaacct gaaattagtt tagctatcgt actctttccg cttcctgatt ttcctactaa
     2461 tgcataaatt cttttttgtt ctaaaactaa gttgaaattt tctaaaacac actcttcttt
     2521 gtatgaaaag ctgacatttt caaatgcaat gtcacaattt ttaatctgtg tctcgttccc
     2581 atattttagt tggtcttgct tcatttctaa ataaagggag tctagtgttt cgactgcata
     2641 tcttgcttca aacaagtgca tacttacata cattattctc atgaaagaaa caaaaataat
     2701 tccacttaaa aaaaacaaca tcaacaagtc aacagctaat atcttgggag tggtaagaga
     2761 agttataaaa aaggttatag gaactatcaa aatgcaaact atgccaaaaa atagccattg
     2821 aaaaagaaca aaaggtgttt tcccactcaa agaatactta tatgcaaact tagcataatc
     2881 gttaattgct ttattcaagg ctttaaatga tgttacttta gcaccaaata tctttataac
     2941 ttgaattcct cggatatatt ctaccgtttc agagcttaac acatctaacg attcttgata
     3001 tttttgaata aaatttgtat tctctgtcat tttctttaac aagaatacac caattactaa
     3061 catggcgatt agaaccaatc caattttaaa attaatgaaa aagcctaagg caactgataa
     3121 aacaggtgtt actatggctt gagcactatc tggaatcaag tgagcaattg ccatatgtgt
     3181 tttagccgcg ttgtcatcaa ttgtctttcg aatatatccc gaagaattta aatcaaaaaa
     3241 acgaaaactt gcttctgtta aaccgctaat tccctttttt ctcaaattag tctctaagcg
     3301 aaacccaagg atatgagaaa ataagccaga aaaaatataa aataaagctc ctaaagttaa
     3361 ccaagcaaca gtttgaaccg cataactctt agctaaatat tcatctccta caataattag
     3421 agacttaaga aatttgtaaa tactgtaata tccatagact gttaatacga cagaaaaatc
     3481 agaaaataaa gtggctaacc atccgtagta tttcatttta gggacataac taaataattt
     3541 tttgtatact ttcatttttt cctcccccgc attcaatttc ttctcttatt cgtttacaac
     3601 tgttaatgct ttccgtaata gcgtaaatta gagatgtaca aacagataga aggtaaacta
     3661 cgttctattt tttgtatttt tttaagactt ttcttccaat caatacacca atcatagata
     3721 aacttgctac aactaacact gaaatgattc cccatatacc taccgcatag tggatcattg
     3781 catccattgt tgcagcagat tgtcctttat ctatccaatc ttgacgaaaa cgatctgtaa
     3841 agaaccatat aggaatccac tgtccggcag cactgcctaa ttgcattaga gcccaagacg
     3901 caacttgagg aattgctcga tcatatccaa atacagatag aataatatca gctaagatcg
     3961 ccatactaat cgaactgata aaccaaggta agtacgcccc gcccattaac gtaaaaaata
     4021 acaataaaac aattgtgtaa atagtaaaaa ttccttttct agggtagtta tagctaatat
     4081 aaacaaatat gattccagat aataatccaa aaatccctgg ggatatgctg tgaccaaacg
     4141 ccccaccaat actactaacc attattgaaa aaaagtaaat tactagggaa ataactgtag
     4201 ctaaaccaat aaaaatataa tcttttgttt tcatatatat tctcctcaat tctatttcgt
     4261 tatgtcatta aaaatctgtt ctatcaaagt tgaatcttct actctaagtt ttatatcttt
     4321 ttttattcca ccatttttta aataaataat tctattgcat gttttataaa gaaattccgg
     4381 atcatgagtg gctataacta ctgcaccctt ttcttttaag tctttaataa ggtcagcaat
     4441 cctttccata ttaatataat ccaacccact tgtaggttca tcaaatagaa ataaattagt
     4501 ttttgacaat gaagctatag aaaccaataa tctttgtttt tctccaccgg ataaatcaaa
     4561 cggtaattga tctttcattt tataaagttc catttttttt aaaatttttt cttgctgatc
     4621 tttactaact atttcattcc ccagggctaa ctcgctcctc actgattctg tgaaaaattg
     4681 gtaatccatt tcttgcatta ctaaaaacgg cgatgacaaa ttttttttat tttcgattat
     4741 ctttcctttg gtagggcgaa taatcttcat gagtattctt agtaaggttg ttttccccac
     4801 accgtttttc cctaccaaac caataacatc tccaactttg acatcaaaat ttatgtcttt
     4861 tagaatattt tcataagtaa tttcttcaac ttttaataca tctgcactta ccttttcgat
     4921 atagggaact tcaatcgaac tattaaaaat gtcaaatttc cttaatgcgt tattgttttc
     4981 agtttttaac aactgaatag ttgtagtttt tacagttcta tttttaacaa atagaacttg
     5041 atcaattaat tctttcaaat agtaaaaacg atgttcagct ataacaatag taaatccttt
     5101 attttttaaa tcacaaagta agtcagcaag tttcttagtc gctttgaagt ccaaatttga
     5161 agacggttca tccaatata
//
LOCUS       DAZKOR010000130         5148 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000130 DAZKOR010000000
VERSION     DAZKOR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.134"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..357
                     /locus_tag="ACG8ZM_002627"
                     /pseudo
     CDS             8..357
                     /locus_tag="ACG8ZM_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(629..919)
                     /locus_tag="ACG8ZM_002628"
     CDS             complement(629..919)
                     /locus_tag="ACG8ZM_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493542.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1321..1557
                     /locus_tag="ACG8ZM_002629"
     CDS             1321..1557
                     /locus_tag="ACG8ZM_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493543.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1564..3516
                     /locus_tag="ACG8ZM_002630"
     CDS             1564..3516
                     /locus_tag="ACG8ZM_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0493544.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3588..4085)
                     /gene="dfrG"
                     /locus_tag="ACG8ZM_002631"
     CDS             complement(3588..4085)
                     /gene="dfrG"
                     /locus_tag="ACG8ZM_002631"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0493545.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4412..5104)
                     /locus_tag="ACG8ZM_002632"
     CDS             complement(4412..5104)
                     /locus_tag="ACG8ZM_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0493546.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1661 a          897 c          858 g         1732 t
ORIGIN      
        1 tacttaattg ttaaatacct tatttgatgc aaaagaaatc tacgatagtc aacaaaaaaa
       61 ttttcattat atccagaaaa atttttctta gttaaggacc tatggattgc agtaatgcag
      121 aattcgtcga ataaactgcc aaagacatac aatcatattg cttttaaaat tgacgaacaa
      181 gatatagatt cgtttgtctc aaaaatccaa atgttaggtt taactgtcga accaggacgt
      241 tctcgtgtga aaggcgaggc ttcttcaatt tacttttacg actatgataa tcacctcttc
      301 gagttacata ccggtactct ccaggaacgt ttaaaatcct acaattccac tacctgagac
      361 tagtaatgac tagtctcttt ttatggcctc tgatcccccc attgagcgaa tactaatagg
      421 gcttcgaatt gatctgttca atctttaagc aatatccctc aaatgagttt ggaaagttta
      481 gtgattcatc acaaatactt aaatacatgt agttatatat agattgaggt tcttgtgagc
      541 gtatgctcgt gtgggttcaa atcccatcgt ctgcatccat ctttccaaaa caagcggcta
      601 ggacattttt gtctcagccg cttgttttct agtttattct ttcatcttgt cggacctttt
      661 ttcttgggta tagacttttc tcatattcta ccacaatttt gggtttccct aacacatgat
      721 aggatctcac aacaaattgc tttctattat tataataacc tgtaacgact aacttcatcc
      781 cttcgtctac ttcagctagg aagttcaaac tatgggaagc aatcaaacaa ttgaccccat
      841 ttaaactgaa acggatcaac ggtctggcac taacttgcaa agtacggatc ttatctactg
      901 ttccatattt tttctccata accttcacct caataccatt atacgaacat acattccctt
      961 tgtaaagaag aaactctgtt ttatcttttt gactttttgt ctattacctt tcttaaaatc
     1021 atattggcac ttcctgcgaa gatgaaggac atgtactaaa aaaaagaggc agcagtatag
     1081 atttaagtag ttcagttttg gagtacaaaa agtgaaaatg taaacctaaa tgagtatcaa
     1141 attgcaactg aatttgatac tcgtttttta ttgttaaatc aacgtttgtt gttaatttac
     1201 accacaaaat gtaagttaaa atgtatataa aaatgcgaac gtgaaataat tattgtagga
     1261 atgcaaattg gtgtgttaaa atggttacaa tttactaagc aaaaaggggt gcattttcct
     1321 atgcaatttc ttgagccaaa aaataagaac gctaagtctg tggattggga aatatcagag
     1381 caagtaaggg tgatagttaa gcaatacgct gaatatgcag agcgtactga aagcgaagca
     1441 gtggatgaat tcttattgaa cattttagac gataagaagt ttattgaatg gattgccaac
     1501 aagcgaagta ataagcggat tgttgaaaaa atgggtatca aagatagagt gggttgatag
     1561 acaatgacca agcttaaaag attagccacg aaggaagatg aaattgtaga tgttaaaatt
     1621 ccgatttcaa acgaagaatt aaaagatagg gctaaacaat atgatttatt aactccaaaa
     1681 cgatttgcaa caaggtataa caaaatgttg ttcttgccaa cgtcttttaa atggaacggt
     1741 tctgaatatc ccattcaata taattactgc atcaatccgt tttgttgtaa ttttgggaaa
     1801 gaacagcaca aattcaaaga tgtaaaaggg aaaccaagtc gatataaaat gactggtagt
     1861 tccaaagata aaggtcataa aggtatgtac tgcaatgaca atcctattgg cagaggtgta
     1921 tcgcaaaact gtacagtaac ccctttatct aattggtctg ttgttgaaga aattaaacgc
     1981 cttatagaaa tcaatagcat ccaagatgtt gagccagatt atcaatttca taaagaaggc
     2041 tgctcggaag aagaatcaac cccattcaat gagccgaagc aattttacaa aagggggaaa
     2101 agtaggggca aatctcaacg ctatcaatgt aaggcgtgta agaagttcac taatgttcta
     2161 ccaaaaagag aagaaaccac tacttatcac caacaaaaga acaccattct tccaatgttc
     2221 gctaagatgg ttgttggaag ggtttcagtc agtcgtactt gtgatatttt aggaattgga
     2281 gttggaacct actatcataa gctggagtgg ctgtataggc gttgtttgga gtttttagaa
     2341 cgatatgaga cacaacccct tcaaacaaag aaatttaacg agatgtggct taatacggac
     2401 aagatgcact actacctaaa taacgtccgt aagaaaggac aaggttcaaa gaaatatacg
     2461 gggtttgaag accttaatat gcaaacgtat atagttgtat ctgctgaggt cttatcaaga
     2521 tatgtgttcc gttctgatgt tgcctatgat tggaatattt caatggatga attaaatgaa
     2581 gatacaagaa aattcaaaga agaccatctc aatactttta gtcgtaaaaa tgataggtta
     2641 gattggtctt attatccaca agaaccatcc gctaacgata gcgaaaatag gaatgcttat
     2701 cttcacgaat tagggaaaat tactaatagg agtagatttg tcgatggttt aaatgttgat
     2761 gctccttata ctacaactgc tcactattgg ttaattaaac aaatggttaa tgccgacgaa
     2821 tggcgaatga ttagtgatga tgacttctct attagaaacg ccttttatag agtattcacg
     2881 aaagaattaa ggttgagcga tgcacatcat tttatttgcc aagttaacaa gacgaaaagc
     2941 agaaaacaat gcttaaaaga attcggacaa gcaaaagcag aactgctcga ttggggagat
     3001 atcagaggat tcaaaacaaa gtttctacgg actttggcat ctcattatct aactgaatta
     3061 ctaacaagtc atcaattcca cgaagaggct attagtaaag atggagaaag atatcgcaaa
     3121 tatgccgata accctatcaa acatccatta gccactaagg ataaaggatt ttattcagtt
     3181 gattgtagaa cggatttatc tgctctggaa ccaaacgaaa tcgcaaagat gctattaaat
     3241 gttaatgacc attcgaccaa tagctttatt caacagataa gacgttatat atcatctctt
     3301 gaaagaccat taacaacagc tcgtggtgat aagaagagct atatttacgc taacttcaat
     3361 ccgaagtatg ctcaatttgc gattacaatt ctaagaactt actacaactt ttgtcgccct
     3421 tttaaatcag cggataaaaa ggttttgaca cctgctcaaa gattagggat tacagataaa
     3481 caatttgatt ggaaagatat aatttatttc aaatgacaaa aagagcttgg agtaatccca
     3541 agctcttgtg ctttatcttt actgttcttc ttcaattaac gcacccgtta actcaataag
     3601 ttttttcttt catatacatg aaaatagtag ttatacggat ttttatcatt ctttatccct
     3661 ttttgggcaa atacctcatt ccattcctca taattcactt ctggaaaaaa agtatctcct
     3721 tcgaattcat gatgtatttt tgtgatgtac attttctcaa cataagggaa aaacaaatta
     3781 taaatctgtt ctcctccgaa aataaaaatt tcttcttcgt ttttacataa ctcaaaaaca
     3841 tcttctattg aatgaacaat ttcacaacca ttaaaggtaa accccttatc tctcgtcaga
     3901 ataatatttc ttctgtcagg taaggctctt ccgattgatt caaggttctt cctacctaat
     3961 attatcggat gtccctttgt agtattttta acatattccc agtccttggg aatcctccaa
     4021 ggaatgtcat tctctttgcc aatcactcta ttcttatcca tcgcagcaat caaagaaact
     4081 ttcatttata acacagcctt tttaactaat ttaccatcta atttcttatt tctatcaaaa
     4141 aaccttttct cattatcctg cccgttagtt gaataagcaa ttttttctat aagttatatt
     4201 atcataatac cacaaaatca caaattttca ttcaattatc attttcaatt gcattttata
     4261 aatcattttg tatgtaattt ggtatgtaag ttaaaatgaa aaaagtagtc cattttgaag
     4321 tacattttgt cattttgtac tccaaaactg aactacttag tatagattac tgttgcttct
     4381 ttactaattt aggtttactt gttaagaaag attatttacg caaatgttct tcaacaattt
     4441 gctgagattt atcagctgta tcttccactt catcttttgc tttttctaac ttttgaatat
     4501 attgtttcaa gcttgagttt tcagcagttg cttcgtctaa tttcttttgt gtatctgtta
     4561 actgagtttc tacatttgtt ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat
     4621 ttacagtatt ttgtaattct gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat
     4681 ctgcattcag atgattgatt tcatcatttt tttggttgat ctcatctagt ttgacattga
     4741 tttcattttg tttgctttgt agttgtgcat ccttgctgac taattgactt tctaagtttt
     4801 gtttttctgc tgccgattga tttttttggt tttctaattc atttactttt gtttggtatt
     4861 cttggacatc tgcctttgaa tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa
     4921 gatctgtaat cgcttgctct ttatctgtca atgtatgatc aagctgtgtc agattctcat
     4981 tgattgtatc aatatctgtc tctccacccc acaaaataat ttcatttgcg taccgcgtgc
     5041 ctgtgtagcc aattagacat agaaatgcaa cgacaatccc accagtaata aatttctttt
     5101 tcatttgcat agctccttaa tccaatactt ttacatgcta aaagtaag
//
LOCUS       DAZKOR010000131         5087 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000131 DAZKOR010000000
VERSION     DAZKOR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.135"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG8ZM_002633"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="ACG8ZM_002633"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HFQ0493547.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG8ZM_002634"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="ACG8ZM_002634"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HFQ0493548.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG8ZM_002635"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="ACG8ZM_002635"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HFQ0493549.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="ACG8ZM_002636"
     CDS             complement(4028..5044)
                     /locus_tag="ACG8ZM_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0493550.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
BASE COUNT         1337 a         1270 c          909 g         1571 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaa
//
LOCUS       DAZKOR010000132         5084 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000132 DAZKOR010000000
VERSION     DAZKOR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.136"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..848
                     /locus_tag="ACG8ZM_002637"
     CDS             132..848
                     /locus_tag="ACG8ZM_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0493551.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            867..2450
                     /locus_tag="ACG8ZM_002638"
     CDS             867..2450
                     /locus_tag="ACG8ZM_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFQ0493552.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2579..3163
                     /locus_tag="ACG8ZM_002639"
                     /pseudo
     CDS             2579..3163
                     /locus_tag="ACG8ZM_002639"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            3650..4675
                     /locus_tag="ACG8ZM_002640"
     CDS             3650..4675
                     /locus_tag="ACG8ZM_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0493553.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            4924..>5084
                     /locus_tag="ACG8ZM_002641"
     CDS             4924..>5084
                     /locus_tag="ACG8ZM_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0493554.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1880 a          578 c          991 g         1635 t
ORIGIN      
        1 attttaatta ttataaatat cagttatatt aagcagttgt gttatagagg aagaaacatt
       61 atttcaatga ttaagagtcg agagcacaaa cggtagacga tgttattttt ataaaaagat
      121 ataatttatt aatggtcgat aattatgaaa ggaaaaatat tcttaaaaaa aagagtatag
      181 gatgtcgtat gaaaatagtc gctatagtac caatgaagtt aaataacaaa agattgccac
      241 aaaaaaacac gaaagctttt accaatggaa aaccattgtg ttattatata ctttcaacac
      301 ttttaaaagt aaaacagatt gataaagtgt atgtctattg tagtaaccca gatattaaag
      361 aatttatacc agaaggtgta acatttttaa agagatctag ttccttagat caggatacaa
      421 cgagtattac acaggtatta gccggtttca caaaagaagt agaggctgat gtatatgtaa
      481 tgacacacac aacagcacca tttattagcg aaaaatctat tgaaaaagga ctagatgccg
      541 tgctgacagg agaatatgat tctgcatttg ctgcaaaaaa attgcaagat ttcttatgga
      601 aagacggtga accatttaat tatgaattgg acaatattcc tagaacacag gatttgccgc
      661 ctttatacga agaaactagt ggattttata tttataatcg caatgtgatg accgagttgc
      721 atagaagaat tgggaaaaaa ccttgcatag ttgaagtagg tgagattgag tcagttgata
      781 ttgatgaagc cgaggatttt atgattgctg atgcgattta taatcatctt attttacaag
      841 gtagttaaga cagggggaag gttaggatga acaatgtaca gattttagat tgcacactta
      901 gagatggggg ctattgtaac aattgggaat ttggatttga taatacaaaa aaaattattt
      961 ctggacttat agaatcagga attgagatta tagaatgtgg gtttctcaca aataaaacag
     1021 tatacaataa aaatgttaca aaatatacga ccttagaaga attagcacag ataattcctg
     1081 aaaaaagaga aggaaaatta tttgtagcaa tgatgaacta tggcgaatat aatatagagg
     1141 atcttccaga ttatgatggt tcatctattg atggaattag gattgcgttt cataaaaaaa
     1201 attgcaatga agcgttgaaa ttatgtcgtg agataaagaa aaaaggctac ttagtttttg
     1261 ttcaagctat ggtatctctc aattatacag atgaagagtt tttagcagta ataaaaaatg
     1321 tgaatcagtt taaaccatac gctttttata ttgttgatag ttttggaatg atgaaaggaa
     1381 aagatttaac tcgtcttttt tacatgattg agcataatct agaaaaagat atacagatag
     1441 gttttcactc acataataac atgcagcttg catattctaa tgcacaaaaa cttataaccg
     1501 tacaaacaaa tcgaagcctg attattgatt cttcggtgta cggaatggga agaggagcag
     1561 gtaatcttaa cacagaacta tttgtagaat atttgaatga taatgcagga aaaaaatatc
     1621 aattaaaacc attattatat ataattgatg aaatactaaa tagtttctat cagaagagtt
     1681 attggggata ttcattacca aactatattt cagcagttca taatgctcat cctaattatg
     1741 caagctatct gtcatataag aatactttaa caattgaagc aatgaatgaa atattcgaaa
     1801 tgatggatga agaaaaaaag gtatcttatg ataaacaata tatagaagat ttatactaca
     1861 aatatatgga aactgggaaa atacaggagg aatatatgga agaactgaga aaatttctct
     1921 cagacaaaaa aattctactg attgcaccag gaaaaagttc tgtagatgag gtagaaagaa
     1981 taaaagagtt tgttaccaaa gaagaagtcg taataattag tgtaaatttt gaatattctc
     2041 atatagatat agattacgta tttttaagta atttaagacg ttttcatgaa ttaccgaaag
     2101 aaagcagaac taaatgtatt gttacatcaa atatacaagc atcggatgta tatttacgga
     2161 cgagttataa aaagttgtta tctgaaaggg aagttgtaag agacaatgca ggattaatgg
     2221 caattagatt tttagccgat atgaatgtgg atgaaatatt cttagctgga tttgatggat
     2281 attcacataa tgaagatgag aattatggtg agcaatccat ggaaattata acaaaatttg
     2341 cattattaga tgcaatgaac gtgcaaatga gagatgaatt gtcagaatta gctaaaaaag
     2401 taaaggtgac atttttaacg gatccacgtt gggttcgaat tgataagtag tagtaataat
     2461 gtttttaaaa ttttgataaa gagatagtga gagaataaaa atgggtaaag tcttaaataa
     2521 aaaagaagaa aaatatttaa gggccgatgg ggcgagatga aactctaatt ttgaactttt
     2581 aagaatcatt tctatattgt gtattgttgc ttaccatgct aatttgcgtt taaattataa
     2641 tgcagacgga attatagtac atggttcatt aactgctaac cagattttct tgaattatat
     2701 tggtatttgg ggtcagctag gagttaattg ttttatattt atttcggcat attttttaag
     2761 tgagaaaaga aacattcact ttgaaaatgt tttgggaatt gtattagaat gtatttttta
     2821 tagtgtatta gtttatggaa ttctttgtgc atgtggaata gaaacatttt cattgaacag
     2881 ctttgtaaaa gaaatattat catttgctct aggtagatat tggtttatta catcctattt
     2941 agcattgtat attctaagtc cgtatttaaa tatcatgctt aacaatctgg aaaaacttca
     3001 gttgcaaaaa ataagtatgt tattgtttat aggtgtatgc tgttatcgta tgttatttaa
     3061 aacatctttg tttggggatt gtgctgtttt aattgcacta tatgtcatat gttatacact
     3121 gaaaaaaacg ataataatat ttttgagaga cacagaatca taggaacaat tgtatgtata
     3181 acagttatac cacttctcat atccatagga atacttttca aatttatacc aaatgcaatg
     3241 gttaaccaga tggttcatat tggttctcca ttgatggtta tagcaagtat atttttattt
     3301 tatgtattta aaaatttttc attttacagt aagtatataa ataaacttgc gagaaccagt
     3361 ttaggggtat atattttaca tgaaaatcat tatttcattg atggtatgaa gtctagaggc
     3421 ttaatatggg atgggatatt tggattaggc aaaaggtttt tagataggga tttttggatt
     3481 tggtttataa ttgcagttgt agttgtgtgc ataatttgca tggctatcga tgttgtaaga
     3541 gaaaaatttg aaagacagat ttttaaaatt aagagtgtaa agaacctgtg taaacttatg
     3601 aatcatgtta taaatatata cccataatta ataaatgagg agaaaagaag tgaaagttat
     3661 atcattcgga gaattattat tgagacttgc agcacccggt tattcaaagt tatttcaaag
     3721 agatagtttg gagactacat tctgcggagg ggaagctaat gtagcggtat ccttagcaat
     3781 atttggatat caatcagaat ttataacaaa gcttccggat tcagatgttg gatatgcggc
     3841 agctaatgct ttgcgttatt ttggagtaaa tgttgataat attatatttg gagagggacg
     3901 catgggactg tattatttag aaaaaggagc agctcagaga ccatctaaag taatttatga
     3961 cagaaaattt tctgcaattt cgttagctga tagtgatgaa tttaagtgga aggagatcct
     4021 aaacgatgca aattggtttc attggacagg gataaatcca gcattgtctg agaatctctt
     4081 tgagacgtcc ttagatgcat gtcaggctgc caaaaaaaga ggtataacaa tttcttgtga
     4141 tttaaattat cgatccaagc tttggacatc agaggaagca aaagaaaaga tggaacagat
     4201 actaccattg gttgatattt gtatttgtaa tgaagaagat gcagaaaaag tgctaggaat
     4261 taaagctgag catacaaata ttaatagtgg agaattaaat aaagatggct atgtgcaggt
     4321 ggctagggag attcacaaga agtttggatg taggtatgta gctacaactc tgcgtagaag
     4381 ttattcggca agtagaaatg gttggagtgc aatgttatac gaagcagaaa aagataaagc
     4441 gtatttttcc acagaataca acatacaaat tgttgatcgc gttggtggtg gagacagctt
     4501 ttctgctggt ataatatatg cgttatgtga aaatatgtcc ggacaaaagg ctattgaatt
     4561 tgcgactgca tctagttgtt taaaacatac tatagaaggt gattttaatc gaactacaat
     4621 tgaggatgta tataatttaa tgaatagcgg tggaaatggt cgcgtgttgc gttagtcaac
     4681 atataaagat aaatcacagt aatttttatg gtgattatct gtcttagtag tgattagaat
     4741 aacgtgaaaa taaattgttg agcgtttata ctgttttgaa aaataaattt aacatattga
     4801 aaaatacaaa tatatacgta tattataaag cgccgattgt aaaattaagc tagacaacta
     4861 aaacagtcat ttgtgctaca ctcaaaatag ttccgaccaa agaattataa ggagtgagtg
     4921 caaataacct atacctatct tacaacggat gaacttgtaa taatagagtc ttatttgaaa
     4981 attaaccaat ctgttgctaa aactgcgcat tgcttgaatc gttcaagtca aacgatccat
     5041 aaagtatatc tgttcttcaa gcaaagaaaa tcagccttag aata
//
LOCUS       DAZKOR010000133         4977 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000133 DAZKOR010000000
VERSION     DAZKOR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.137"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACG8ZM_002642"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="ACG8ZM_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0493555.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="ACG8ZM_002643"
     CDS             complement(2066..3064)
                     /locus_tag="ACG8ZM_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0493556.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="ACG8ZM_002644"
     CDS             complement(3290..3943)
                     /locus_tag="ACG8ZM_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0493557.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACG8ZM_002645"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="ACG8ZM_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0493558.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1479 a         1031 c          761 g         1706 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt atacataatc aacttttaaa tagctacctt tttttcataa tttttttaaa
     4801 aaaatgaaag ttatctaagg ttaatataca gtaaaaccag cattctcgct ttttttatca
     4861 tttaaaaatt agagttttca aatctaattc aaattttcta aacatatgaa acagcgtata
     4921 aaactgtact attattttat ctcaaaagct aaatgaaacc gatttcttaa gattttt
//
LOCUS       DAZKOR010000134         4859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000134 DAZKOR010000000
VERSION     DAZKOR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.138"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..536)
                     /locus_tag="ACG8ZM_002646"
     CDS             complement(<1..536)
                     /locus_tag="ACG8ZM_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0493559.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEI"
     gene            1230..1616
                     /locus_tag="ACG8ZM_002647"
     CDS             1230..1616
                     /locus_tag="ACG8ZM_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0493560.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1714..1905
                     /locus_tag="ACG8ZM_002648"
     CDS             1714..1905
                     /locus_tag="ACG8ZM_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493561.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            2419..2751
                     /locus_tag="ACG8ZM_002649"
     CDS             2419..2751
                     /locus_tag="ACG8ZM_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0493562.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2661..2864
                     /locus_tag="ACG8ZM_002650"
     CDS             2661..2864
                     /locus_tag="ACG8ZM_002650"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0493563.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            2895..3041
                     /locus_tag="ACG8ZM_002651"
     CDS             2895..3041
                     /locus_tag="ACG8ZM_002651"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493564.1"
                     /translation="MTDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            3048..3203
                     /locus_tag="ACG8ZM_002652"
     CDS             3048..3203
                     /locus_tag="ACG8ZM_002652"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFQ0493565.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            3479..4819
                     /locus_tag="ACG8ZM_002653"
     CDS             3479..4819
                     /locus_tag="ACG8ZM_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0493566.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         1699 a          869 c          796 g         1495 t
ORIGIN      
        1 tatatttctg gatcgtaatt ttgtgataac agattatcta ttttgatttg ctcgatagtt
       61 ttacttagat ttcctgtata ttcaattaac ggttcatgga atagatcttc tatttttcca
      121 gtaaaaaaat aatgaactaa aatcagaata tctcgaatgt ctcctaaaga atacaaagtc
      181 aaattttgca atgaattata gagtcgttct ctatcagcca ttgcaacatt caaattgttc
      241 atttttagtt taaaaaaatc tttatcaata ttatttgctc taaatggacc aaataagtaa
      301 tatctggttt taaacatgta tagaagaaac atctcattaa aatcaccagt aaagaaatgg
      361 aaatctttag tttttttaat attatcagtt agtaactttt tataatcata aaaaaaataa
      421 gtagttctat ctgagcgata ctcttttata atactgaatt cggaattaaa aaccacgata
      481 ggtaacttta aacaaacatg aagagacttt aataagactt ctttatctga aaacactttt
      541 gtttctctct ttcctttcta catcattaaa tagtatttta tacgttatct aaaatactat
      601 tcctctgtca aaataagcta acttacttca ttataaaata tccacttatc gctttcatgt
      661 atattccacc gtaaaaagta cattttatca ccgagaactc atcatttttc atcatgatga
      721 atactattta catatatcta gttcaaagac aataattatt atgatccgtt attatctaag
      781 cactatacag ataataggaa tttttcgttt ctaaatactc caatttggga atccaataaa
      841 aattttcgtt caaatacgtg tctattcgaa ctcttttcta gttgttaatt atgaaggggg
      901 aagaaataaa aaatacaatc tatacacgaa taagaatatt atccaaaagg aggttttaga
      961 aaagatttat tatacgtaaa aagctcaaga cattttctta gccataagga catgtttgat
     1021 atttaagaag atatatgaat tttcttttct aagtataaag ataactatcc attatcaact
     1081 aaaaaaacat ttttgaaaat aaacatcagc agtcctatca atgcatgtga gcttatagaa
     1141 gttattcgca gcgcagcata attgcattag cttcttttcg tgttttttgc ctcttttccc
     1201 tttaaatctt aatggaggag gtgtgcaata tgtcagataa cgacgaacgc gtaaatgccc
     1261 agttgattcg attctttgaa aaggtcattt tgaacaccgc taatacttat tataaaaaac
     1321 aagaagaaat tcaaaaacat gaacaacttg atcaactccc accatatttg tttactaaag
     1381 atactataaa atttaaacaa ccgattaatg ttttaaatgt gactctcctt gttgaagata
     1441 taaaattagc aaaaatcctt cctttgttga aagaacctga acaaattttt cttatcgaaa
     1501 aatttatctt taataaaact gataaagaaa ttggaaaggt tctgggtatt acaagacagg
     1561 gtgcgacaaa tttaaaacat cgactttaca aaaaaattca aaaaatgctg gtttaatatc
     1621 tgcataataa ctaattttgt atgcttcttt gctcgtcctc ctccttcccc aaaaaataaa
     1681 tagattataa gttcatggga agaaggtaaa tttatgaata ctttaaaaga agatttcatt
     1741 ttagcaaaga atggaaatga agaagcagtt gtggccattc tcaaaagatt tagcgctttg
     1801 gtccataagc aatcctggag aactggaaaa tatgatcaag attgttatca agaatgtatg
     1861 cttgcaatat atctagctat ctcgaaattc gaaattaaag aataactcaa ctaagggagt
     1921 ctgtgacata agtaaaaaga tagtttcaaa tttcgaacaa taaatttgaa gccatcttct
     1981 ttataattat aaagtatcga ttttacagcg ttctataatg attcatagaa tctaaaacga
     2041 atgattttaa ttattaacgt tttttaattc gtttttagct taaaaataac ttttgtcaca
     2101 gtctcactcc taaaattact ttatttacgt acgaagtggg caggaggtcg aatccagaac
     2161 tgggccatgg tacttcaacg aggacttctc ctctagaatt gaaaagcatg cgatttacct
     2221 tccgtaacga cggtagattt attcaactat gtgtccaatc aaaatttgtc tgatttcaaa
     2281 aatagtcaag agaattttgg ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt
     2341 gacaaaccct gatactaagt tgggctatga tacacttttc ttgactatca gttacgttac
     2401 cggaaggaat gaactctatt gaaaaaaaca tatgatcgta aatttaaact aaagatttca
     2461 caggatatcc ttgagaaaaa agtaactacc aagaaaattg ctgaagaata caatatctct
     2521 cgtccaacaa tttcccgatg ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct
     2581 ggacaaggaa aaagattacc tgataaagca gatttctaca ttcttgaaca ggaaaataaa
     2641 cgactaaaag aggaagacga gtgttctacg aacacaaaga tcgatacgga tctccaggga
     2701 tcactcaaaa actttacgat gaaggaatcg aaatcaataa acgtgttgta gctgttctta
     2761 tgcgtgaaat gaatttatgc acaagaggat ttcatcgacg caaatcttct catggtagag
     2821 aaaaaacaat caaagaaatg gtaaaaaaaa tgttgaatcg ataatttgaa cagaaccaaa
     2881 ttgatgctgt ttgggtgacg gatattactt acattccatg taatgacggt cgcctctatt
     2941 tgtccaccta tattgactta gcaactcgta ttctacgtta ctatatggtg gattcacaca
     3001 tgaaaaaaga gattgtcgtc cgtccattac aaatttatta aggagaattg cctacggtaa
     3061 ttcattccga tagaggtagc cagcatcggt cttttattta tcaagagctg ttgaccgacc
     3121 accacatcac acattcaatg agccaaactg ggactcctgt tgacaatgca gtaattgaag
     3181 cgtttcatcg atcaatcaaa tgagaattaa tcgactccaa caaatataaa agtaaaatag
     3241 agatgaaggt gttaatccaa gattatttag aagactacta cccaaacaaa agaatccata
     3301 caaaatttat gatgactcct tgacaatttg aagcgaagaa aataagggat ctataatatc
     3361 tagtgttggt gacgccttct gttgccaata ctttttcttt tttctaataa acgcaaacag
     3421 acaccttgaa atcctttaga ataaagtcga cgaaaaccat tcaaaggagt gtttcaagat
     3481 gtcctattca gagtttacca aagagttact agatattcta gacctaaatc ttacctttca
     3541 tgaagatgcc tttcgcaaag agcgcattaa tgacgaaaca tgttttgttt ttgatggcac
     3601 attgacctat caaccagagg aatgcttcca ttgccactat caaaataagc aaacgatcat
     3661 taaatggggc tggaaaaaag tatccatctt attgaatgat gtcagtaact acaagaccat
     3721 tgtgcggatt aacaaacagc gcttcaaatg taaacactgt ggaaagactt ttttagcaga
     3781 agattcagtt agcgatcgtc gctgctccat tgcccgcaga gtcaaacaag ccattcttga
     3841 gctattaagt gagcctattt ctatgtcttt gattgcacgt atgaagcaca tttctccgac
     3901 cactgtcatt cgaatacttc gcagccttcg gcctaaaacc gtttctttga accagccgtt
     3961 accagaagtg gtgtgtttcg atgaattcaa atcggtgaaa aacgtctcgg gagccatgag
     4021 ctttgtcatg atggacggca agacccatca actgattgat attgtggaaa atcggcaact
     4081 caatccttta cgcgactatt tcttgcgcta ccctagaaaa gtccgtgaac aagtacgtct
     4141 agtggtttca gatttctata caccctatcg cacattggtg aaagagtgtt ttccgaatgc
     4201 ccgaatcgtc gccgatcgct ttcatattag ccaacacatc ggtcgggcct ttaccaacca
     4261 ccgaattcaa gtaatgaaga cctttaaaaa aggggatcgt cggtcgaagc acttaaagaa
     4321 atactggaga ctacttcaaa agaatgcgtg ggaattgaat ggtcagcatc gctactggcg
     4381 tccgtcattt cgagatcatc tgacagaagc ggagatagtc gatcggctat tatattatga
     4441 tgactcctta aagcgaggct acgaagtcta ccaggttttt ctttccgcta tcagaagaca
     4501 agatgttcct gaattcgtcg cgctcctaaa agaagactac aaggagttgc cagagcacta
     4561 tcaaccagtg ttcacgacct tcaagaaata tcgaacagaa atcaaacgag cgttgcgagt
     4621 tccctattcc aacggcccta tagaatgttt gaacaaccat atcaaagtac taaaacgaat
     4681 tgcctatggc tttagaaact ttcaaaacta ccgagaacga atctttttat atcgaggaaa
     4741 atatttcaag aaaactaaga acactaccca actgactaaa gccagaacaa caacacggct
     4801 agacaagata gctgtctagc cgtgtgagta tctcgattat tctattgaat ttcttcacc
//
LOCUS       DAZKOR010000135         4852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000135 DAZKOR010000000
VERSION     DAZKOR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.139"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..2410
                     /locus_tag="ACG8ZM_002654"
     CDS             8..2410
                     /locus_tag="ACG8ZM_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0493567.1"
                     /translation="MLSFSIILSYLIVDLKLCNRTVLSTQKWLNKKYSNVTGFDKVPE
                     NGRTGWPTIYGLIEGLQVELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQ
                     GAFWCKGINPGGFDGVYTPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFV
                     LVPGGDSKIRKMQQWLNVNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATG
                     SYGNGTTSKTYPVSEGNSGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKF
                     MVLPEYTEIADVVVIKGLLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYL
                     TGTVGIGKDERNKYLTTEELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAID
                     AAKNLNIPLTTVIYFAVDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCS
                     QIINNGYASFAFVSDMSTGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSG
                     RDSGFNELSNDTNNDNYIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYS
                     GLSWDIISSPVTEHDRKIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQM
                     FLADTINDSVSDFAGWLGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQD
                     VDAVNVYTLAKRNSDSTALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLE
                     NDETVDILGVGIISFFIDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSK
                     LN"
     gene            2490..2885
                     /locus_tag="ACG8ZM_002655"
     CDS             2490..2885
                     /locus_tag="ACG8ZM_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493568.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3210..3827
                     /locus_tag="ACG8ZM_002656"
     CDS             3210..3827
                     /locus_tag="ACG8ZM_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0493569.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            3868..4167
                     /locus_tag="ACG8ZM_002657"
     CDS             3868..4167
                     /locus_tag="ACG8ZM_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0493570.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            4201..4368
                     /locus_tag="ACG8ZM_002658"
     CDS             4201..4368
                     /locus_tag="ACG8ZM_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0493571.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(4413..>4852)
                     /locus_tag="ACG8ZM_002659"
     CDS             complement(4413..>4852)
                     /locus_tag="ACG8ZM_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0493572.1"
                     /translation="TSQQNPSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNT
                     GNELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTD
                     PQKRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         1649 a          677 c          963 g         1563 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gttttaagta ctcagaaatg gctaaacaag aaatacagta atgttacagg
      121 gtttgataag gttccagaaa atgggcgaac agggtggcct acaatctatg gacttattga
      181 aggactacaa gtagaacttg gtattactaa tctagtagct aattttggac ctaccactga
      241 aaaaatgtac gataatcagg ttactcctaa atggggcaaa aatctaccta aaaatattgt
      301 atttcttatt caaggtgcct tttggtgtaa ggggattaat ccaggtggct tcgatggggt
      361 atatactcca tatttagata ctgctgtaaa agagttgcag actgatgcag ggtttaatgg
      421 aagcgctgta actggagttt tggatgcgaa atgggccaaa gcgttatttg atatgagtgc
      481 ttttgttctt gttcccggcg gagatagtaa aattagaaaa atgcagcaat ggttaaatgt
      541 aaattattta gaatacacgg gaataatgcc ttgtgatggt atttatcaaa gaaattcaaa
      601 tcaagcatta atttttgcat tacaagctga actaggatat tctcccagtg aagcaacagg
      661 gtcttatggt aatgggacaa ctagtaaaac ttatcctgtg tctgaaggta actcgggaaa
      721 ctatgtaaga ataattcaat atggattata tgttaatggt tattttgaag acggtacatt
      781 cgatggtatt ttcactaaat atatgggttt agaagtactg gcattccgaa aatttatggt
      841 attgcctgaa tatactgaaa tagcagatgt tgttgtaata aaaggactat tgtctagcgc
      901 tggagatatt agaagatctg cagatggcgc agatacttct acgcaattaa ctagatctca
      961 aatccaaaca ttggttgata atgatataaa aatagttggt agatatctta ctggtactgt
     1021 tggaattgga aaagatgaac gaaataagta tctaactaca gaggaattga ataatatttt
     1081 ttctaaaaat ttatctgtgt ttcctatcta tcaagatgga ggtgcagcat tagcttattt
     1141 tacttatgat cgtggcttat cagatggtaa gaaggctata gatgcagcaa aaaatctaaa
     1201 tataccatta acaacagtta tttattttgc tgttgatcta gacatgttag gggaagaaat
     1261 tcttgcatat gctggagaat attttaaggg ggtatctgct gtaatgtcat atagcgggta
     1321 tcaaacgggt gtttacggaa caagaaatgt ttgttctcaa ataatcaata acgggtatgc
     1381 ttcgtttgca tttgtttcag atatgtcgac tggttatagt gggaatttag gttttcctat
     1441 gcctagacaa tggtcttttg atcaatttgt tgaatttacc ataggaagtg gcaacggagc
     1501 tgttggtatc gatttaatcg ctacatcggg tagagatagc ggatttaatg agttatcaaa
     1561 tgatactaat aatgataatt acattgccaa atacaatcag aaggttataa atcagatggt
     1621 acgtgcgtat caatatctat cacaagctgg tcttgataat ccttggaacc ctcatttgac
     1681 attttataga tatgtaaatt attctggttt gtcatgggac atcatttctt ctcctgtaac
     1741 agagcatgat agaaaaattt atgatgaata taggaataaa ttaactaata gcgagggcct
     1801 gtataattat tttatagatc ctaatactgg ttcaataata ggtttgcctc acttaattgt
     1861 gacgttacaa tcacagatgt ttctagccga cacaatcaat gatagtgttt ctgactttgc
     1921 aggttggtta ggagatttaa tgacttgctg gggtgaagtt aagaaattag gaatacaaat
     1981 ggagcaaggt gtatttgcat tagtaggtac ggctgcaggt ggaacattta gtctagaaga
     2041 tttataccaa gatgttgacg cggtaaatgt ttatacctta gcaaaaagaa attctgattc
     2101 aacagcttta tcggctttaa aaacgtacta ttgtgataag ggtgctattg atagatttaa
     2161 tcaatttgtt aagaacagat ttggttcttc agataaaatc gcttcagtaa caaatagttt
     2221 attagaaaat gacgaaacag ttgatatctt aggtgtaggg ataatttcat ttttcattga
     2281 taaaatagtt aataattcac ttgggacaga ttttacttat cttgatttta ttggagatga
     2341 ggggaaagtt gcaaatggat tttctgaaaa aattattcaa tttcaagaaa atagaagtaa
     2401 acttaattaa gaatcgttta ctatttaaac agtgtccttt tcctttttaa atatatacga
     2461 taaaataagt atatgaattg taaaaggaca tgatgattat ggaagaaata gaatggaata
     2521 atctgactca aaataataaa aataattctg gatgtggaat tatctttatg tttgttatag
     2581 ttttgggaat agtagtactt ttaataactg tcaagatagt agattacttt gcgaatactc
     2641 ctgaagaaca aatacagaga aaacttcctg agtttgaaag agtaccacct tatgatccaa
     2701 gagataattc atttcaagta aaaaaattaa aagagtctcc aaatgaaatg tggtttgaag
     2761 ttacagttga atcaagcgac ttcaaagaaa aaaagaaaga atacaattat attgtaaagg
     2821 caaaagtatc aaaaagattt atttttaacc ctaaattaga aatatatagt gttgtaatag
     2881 aataatagtg ttaaaaaaat tgtaaaagtt aaagaagtca aattctgatg tgaaaaataa
     2941 aatataccat ggaagacata aaagaaaaac tggataacta gtttatttaa tggcagaccg
     3001 ttaaggcctg ccatttttat gtattttagc ggaataaata taatcatgcg ggagtgtaaa
     3061 cttactgttt caaaaagtat actttttgac atgccttgat tttctttgtt tatgtatggc
     3121 ataataacca cataatacaa tttgaaacgt ttctgattta atctgattgt tttgaaacaa
     3181 ttttagtgaa aaaagggtag ataaaaacga tgagaaaatt tttttatatc cgggtcagta
     3241 gttatgatca atcgatcgaa cgacaactga tttccgcgaa ggaattagga atccctgaag
     3301 aatatatatt tgtagaaaaa gccagcggca aagattttaa acgccctgaa tatcaattaa
     3361 tgaaacggat gtttcgaaaa ggagatttac tctatctaca atcattagat agactaggca
     3421 gaaataaaca aatgatttta gaagaatggc gagaattaac taaggaaaag cagattgata
     3481 tttctgtgtt agacatgcct ctgttagata ctgctagtta caagcaacta gatgggttag
     3541 agacgcttat ctctgactta attctgcaat tactcagcta tatggccgaa gacgagcgga
     3601 aacgaattaa tagccggcaa agagaaggcg tagaagctgc gaaacaaaag ggtgtaaaat
     3661 tcggcagaaa gaaaattgct ttacctcctg aatttcctgc gatctattct gactggaaag
     3721 caggaaagat aactgcagtc tatgctatgg atcaattggg catgaaacga aatacctttt
     3781 atcgacgggt gaaagaatat gaacatacct tgcaccactt ttcttgatag atataacagc
     3841 tattagacgg gaagggagag tttgaaaatg gacttaacga atcaaagggt cgtttttact
     3901 ggaacgctgc agcaaatgac acgaacccaa gccatcggtt tggttcactc tttaaacggc
     3961 cattgtcaat caagtgttac ttcaaaaaca aatttcctgg tttgtggtca gtattctaag
     4021 tcgttgtttg atgattctct ctatacaaaa aaggaacaaa ccgcgcggga cttgattgcg
     4081 ttaggtcatg agattaccat tttatcagaa gtcgaatttt atgcgttaat tagtcaacag
     4141 ttgaaagaat ggcaacggta tttataatcg ttagtaaaat ttacaaggag gaaaattttt
     4201 atgaagcgaa aatcttatga tcgttggggc attgaaataa ctagttttaa tcgaaatatt
     4261 gcttttcacg tggagtgtga ctgtggggaa ttagcaaaaa gtgtttatca aaaaaattac
     4321 ttttactgtc ctaaatgcca tcgccgatac ggggtacatg gaacatagaa acgaaatagt
     4381 gcggtagaaa agcgaatgaa tgtagtatat ttctacaaat cattcgcttt ctttatttta
     4441 taaatagttg gtcgagagat cccgttttct tttgcaatct ctgcaactgg aatgccttgc
     4501 tttaacatgt ccaccacggt tttaaaaact aagcgttttt gcggatctgt tgagttaata
     4561 gaatactcga ttggtcgtcc cttatatcgg ccggcttttt gagccaataa tactccttgc
     4621 cgctgacgtt cacgaatttt ctcacgctcg ttttgtgcaa tataacttag cagtgataaa
     4681 aacatatcga acatagcggt atccaggagt tcatttccag tattaaaatc taaaaactgt
     4741 gcatctaata tttttaacgc tacttttttc tgtttcataa aagcaactgt attttttata
     4801 tcttcataat ctcttcctaa acgatctagt gatgggttct gttgcgaagt tt
//
LOCUS       DAZKOR010000136         4808 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000136 DAZKOR010000000
VERSION     DAZKOR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.140"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="ACG8ZM_002660"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="ACG8ZM_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0493573.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1523..1718
                     /locus_tag="ACG8ZM_002661"
                     /pseudo
     CDS             1523..1718
                     /locus_tag="ACG8ZM_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3250..3768)
                     /locus_tag="ACG8ZM_002662"
     CDS             complement(3250..3768)
                     /locus_tag="ACG8ZM_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0493574.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4135..4383
                     /locus_tag="ACG8ZM_002663"
     CDS             4135..4383
                     /locus_tag="ACG8ZM_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493575.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1579 a          786 c          730 g         1713 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcgtta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccacttag
//
LOCUS       DAZKOR010000137         4773 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000137 DAZKOR010000000
VERSION     DAZKOR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.142"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACG8ZM_002664"
     CDS             complement(207..1169)
                     /locus_tag="ACG8ZM_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0493576.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACG8ZM_002665"
     CDS             complement(1171..2610)
                     /locus_tag="ACG8ZM_002665"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0493577.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ACG8ZM_002666"
     CDS             2795..4741
                     /locus_tag="ACG8ZM_002666"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0493578.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1517 a          818 c          789 g         1649 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tct
//
LOCUS       DAZKOR010000138         4716 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000138 DAZKOR010000000
VERSION     DAZKOR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.143"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..190)
                     /locus_tag="ACG8ZM_002667"
     CDS             complement(<1..190)
                     /locus_tag="ACG8ZM_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493579.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="ACG8ZM_002668"
     CDS             complement(200..1015)
                     /locus_tag="ACG8ZM_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0493580.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="ACG8ZM_002669"
     CDS             complement(1277..1720)
                     /locus_tag="ACG8ZM_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0493581.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="ACG8ZM_002670"
     CDS             complement(1854..2192)
                     /locus_tag="ACG8ZM_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0493582.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="ACG8ZM_002671"
     CDS             complement(2180..2557)
                     /locus_tag="ACG8ZM_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0493583.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="ACG8ZM_002672"
     CDS             2781..3974
                     /locus_tag="ACG8ZM_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0493584.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="ACG8ZM_002673"
     CDS             complement(4140..4562)
                     /locus_tag="ACG8ZM_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493585.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         1376 a          898 c          824 g         1618 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4621 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4681 aagtattcta ttgatatgaa tcatttaatc gtttat
//
LOCUS       DAZKOR010000139         4633 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000139 DAZKOR010000000
VERSION     DAZKOR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.145"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..516
                     /locus_tag="ACG8ZM_002674"
     CDS             172..516
                     /locus_tag="ACG8ZM_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0493586.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="ACG8ZM_002675"
     CDS             517..936
                     /locus_tag="ACG8ZM_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFQ0493587.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1028..1378)
                     /locus_tag="ACG8ZM_002676"
     CDS             complement(1028..1378)
                     /locus_tag="ACG8ZM_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493588.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="ACG8ZM_002677"
     CDS             complement(1344..2675)
                     /locus_tag="ACG8ZM_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0493589.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="ACG8ZM_002678"
     CDS             2931..3497
                     /locus_tag="ACG8ZM_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0493590.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="ACG8ZM_002679"
     tRNA            3642..3729
                     /locus_tag="ACG8ZM_002679"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..>4633
                     /locus_tag="ACG8ZM_002680"
     CDS             4063..>4633
                     /locus_tag="ACG8ZM_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFQ0493591.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1683 a          778 c          891 g         1281 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggcaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaatttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat agtagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaacga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggaggggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaa
//
LOCUS       DAZKOR010000140         4587 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000140 DAZKOR010000000
VERSION     DAZKOR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.146"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACG8ZM_002681"
     CDS             186..2855
                     /locus_tag="ACG8ZM_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0493592.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACG8ZM_002682"
     CDS             complement(2946..3560)
                     /locus_tag="ACG8ZM_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0493593.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="ACG8ZM_002683"
     CDS             3680..4219
                     /locus_tag="ACG8ZM_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493594.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACG8ZM_002684"
     CDS             4216..4554
                     /locus_tag="ACG8ZM_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFQ0493595.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1419 a          790 c          860 g         1518 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggaggaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgac
//
LOCUS       DAZKOR010000141         4461 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000141 DAZKOR010000000
VERSION     DAZKOR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.147"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            574..1008
                     /gene="bacA"
                     /locus_tag="ACG8ZM_002685"
     CDS             574..1008
                     /gene="bacA"
                     /locus_tag="ACG8ZM_002685"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFQ0493596.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="ACG8ZM_002686"
     CDS             2298..3221
                     /locus_tag="ACG8ZM_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0493597.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="ACG8ZM_002687"
     CDS             3237..3776
                     /locus_tag="ACG8ZM_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0493598.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DAZKOR010000142         4455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000142 DAZKOR010000000
VERSION     DAZKOR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.148"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..1435
                     /locus_tag="ACG8ZM_002688"
     CDS             134..1435
                     /locus_tag="ACG8ZM_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0493599.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1462..1722
                     /locus_tag="ACG8ZM_002689"
     CDS             1462..1722
                     /locus_tag="ACG8ZM_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0493600.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2721..3956)
                     /locus_tag="ACG8ZM_002690"
     CDS             complement(2721..3956)
                     /locus_tag="ACG8ZM_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0493601.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1313 a          699 c          901 g         1542 t
ORIGIN      
        1 ttcttaaaaa taaatttgat tatttaagag aatatattga aataaaattt cttaattata
       61 taattagtta tgaacattct ttgcgttgtc atgcaatgaa tggttcatat tttattgaaa
      121 aggagtggtt ttagtggaaa agctgatgaa atgggtcgaa gagaaacttg tcccaccaat
      181 ggcaaaaatt ggtacacagc gtcatttatt agcaattcgt aatggggttg tttcaacatt
      241 gtcattgatt ttgattggga cattttttat ggtctttatc aatttaccgt ttcctggatg
      301 gaatgaattt attgcccctt attcggcaac gattgtttta ccctttagga tgacgatggg
      361 attgatggct atttatgcaa cttttgttat gggttcggat ctagctaaat catatggatt
      421 agatagtgtg acaggtggta tattatcttt aggaacgttc tttatgttac aagttcctgt
      481 aaacgtttta actcctgaag aggcacctct tggctgggtc ttacctatga gttctttggg
      541 agcatctggg atgtttgcgg ggatcttatc tatgattttt gcagtggaag tatatcgttt
      601 ctttaaaaat cgaaatatta ctatcaaaat gccagaacaa gtgccacctg ctgttgcccg
      661 tagttttgag gcattgattc caggtgcagt cattttaaca attacttggc tgattcgaag
      721 cgtcgcgggc tttgatgtaa acgctgcctt attatcgtta ttcgagcctt taaccaatat
      781 tttagggaat aatttgcttg gtgttttact accaatgttt ttgatccatt tgttgtggtc
      841 atttggtatc catggcatga gtattgtagg ggctgttgtt cgtccaatgt ggctgatcat
      901 gttggatgag aatgcaaagg cattggcaga tggtacagct gccacccaat taccctatat
      961 cgctcctgaa cagttttatc aatggacggt aacaattggt ggtgcaggag caacgatcgt
     1021 ggtatctgtt ttgtttatat tcttctgtaa atcaaaattc ttgaaagagg ttggtcgttt
     1081 ttctattatt cctggtatct tcaatatcaa tgagcccata atttttggag ctcctatgat
     1141 gttgaatcct tacatgttca tcccgttcaa cttagttcct ttagttttga ctattatcag
     1201 ttattttgca gtcaaattag aaatggtcaa tagatttact gttttgccaa catggacatt
     1261 accagctcca atcggcggtt atctttcagc aggaaatgac tggcgtgttg ttatcttgat
     1321 catgatcaac acattgatag catttattat ctattatcca tttgtaaaag cttatgataa
     1381 aaagatgctg tcagatgaac aaagtcgtgg agaaaacgaa gtccagactg tctaagtttg
     1441 gtttttagaa ggtgggaaac aatgtattgg ctgattttat tttttgtttt tatcttttta
     1501 ttaacggcta gtcaccttat attgaatatg ctagctacat atcatattca aatcaatcga
     1561 tggatttggg cactggcatc atttttgatc gtgattcttc caaagatcat tgtaccgcat
     1621 atgaatgttt tgttttcatg ggggacatat gtactttgtg gaatttttgc tatcaatttt
     1681 atgatcgagc agcatagatg gtttgtcaca tcgaagcttt aaatgttcta aaagtggttg
     1741 taacagaagt ggttagcttc aagaaataag aagaaattta cgaaaattgc ttcttaaatt
     1801 tttgtgaaat gttggcttat ttttcgtttg gttgaactta ttaccgcaga tgcaagcttt
     1861 tgaccaccgt ttattcggaa tatgggactg tgacaaggct tttgtcacag ccctttttta
     1921 cataaagatt attattgttt taactgtttt ggcagaaaga aggaggaaaa aatggaaatt
     1981 ttatcagcaa tggatttaga attagtagat ctactaccaa agtgatcaag tcaaatatag
     2041 atttgggagc ttacaaccat atagtagggg ctgcagtatg gtgtatgaat ggtaatatgt
     2101 atgatgtgag tgtttattca aatcatgaaa cgtgtataga aataattgtg ctgggaatag
     2161 caatcagtca tggtgaacgt gacttttagt gtatcgttgc tgacgacata gaacattctg
     2221 acatgatcta ctcaatatgt ggggattgct ggcagtttat ataggagtat gcatcggatt
     2281 gcgagatcat tgtctcaaca gaagagggag gttgattatc aaagataaag tggtaaatcc
     2341 tacatttgat ttagtaattg ttgaggggac tagcggcaca aatctctaca aagcgatgct
     2401 tctagatcta tatgacacat tttaagaaaa tgtatttgcc taattattat ggtctttctt
     2461 ttgaagaaac gttgagatga tccaaccaga gaaaggtgtc aaagaatttg actaagaata
     2521 aatggcagac gctacctatg ctcatttgga tagcgtcaga tcttttattt taaggatgat
     2581 tttgtgtttt ttagacaccg taatttaagt aactctaact tttcaatcca ctatttactt
     2641 tttttgtatt ttcactgtct gggtctcgaa tagcgaaatt actgatgagt ctctattaaa
     2701 aaagcttttt ctacttgaaa ctatattggg tggttgctca ggaaggtttc gatttcttga
     2761 attgcttgtt gtggaccatt tattggaaaa acggcttgca gcgtgcgtgc ctgctgacga
     2821 atcttgggtg aagaaacttc gcgcagagca atctgcagtt ttctttcagt cactggtttt
     2881 gtgagggcga ttgcgtttcc gtattttaca caatagtcaa tattacagct ttgctcaaat
     2941 tgcatcccca caccgataaa cggtttgccg gattcacagg cagtttggac ggtcccttcc
     3001 ccgccgtgga taaaagaaaa atcaatgaat tcatgcaaaa gatgactaga cagatattct
     3061 gttattaaaa gatcttctga gtcagattga atgtcatcaa gcagatagtc aataggtgca
     3121 ataaccgcta gatccggttg tttttgtaag aagaacaaaa ttttgcgtaa catacgcgga
     3181 ttgcctgaac tacccattgc gaaatagata atacgttttc tttcttgttg ctttcgctgt
     3241 ataaaagtta gcacttcttc aggcaaggaa gtactgagct tagcaaaaat tggaccaacg
     3301 attcgataat tttccggcag ctgttcttct tccaaaaata gtttaggcgt tgtgatgaga
     3361 tttagatcag catccatcat ttgtaaactt gtttttggta aaggaagcat ttctttttgt
     3421 gcagcgtgtt tgaaggcatt ggggatccat cgggtattta ctatacgctt ttttgtccag
     3481 tgccaaaggg gattgcttaa agggccgaag aaacgtaact cgtggggcat cagcgcatgc
     3541 tctgattgaa aaaaaggttc actcaaagca tagggtttaa catatactaa aggaatcttt
     3601 ttattgcggg ccgataaaaa aattgtcaca ttcgaacctg tcacaatcag tcgaggctca
     3661 aacttagcaa tcagactctg ttcactagtg atacgagagg caaccatttc tttggtaaaa
     3721 gggtttttaa gtgtacgcaa ctggtcaaat tttatgattt gttcttcttg ttttttcgta
     3781 aggatcggcg ctagtaaatg atactcaaag ccactttctg tgatcaaagg agcaaaagtt
     3841 ttagaaaagc caaagaatac acagtaataa cggtctttca tagctttggc aacctcaatc
     3901 attcgtgttg tctcagctaa attgaatact gcaggagcaa aaagaattgt cttcatgaaa
     3961 tcctccttat tggaatgagt ctatcccttc gtttcttact agtatactaa aaaacacagc
     4021 gttcgagtag cctatgtata ttttgaatat taaactgtct ctcgctgttt tcacaacttc
     4081 taaatttaaa ttttgtaatc aagttgaaac tccaattttc aaaaaaacac gcaacaattt
     4141 tttgatcgat ttatatgttg tgccagataa atacgagaag ttgtgtcatt aaaaataaac
     4201 atgagaacat aaaaaaataa aataattata ttaattgata ggtgtgctgt cgtcattata
     4261 cacacgtaag aaagaaatag ttatgtttaa aaatattctt agactttctc gccatgacaa
     4321 ttttcactat gtgttataac tgtaaacgat aattattcag ataacacaaa aagaaatcaa
     4381 ggagcagata cgccagttgt ttaatttgaa tattatttta aaattaaaat ttttattctg
     4441 ttctaaacat ttttg
//
LOCUS       DAZKOR010000143         4450 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000143 DAZKOR010000000
VERSION     DAZKOR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.149"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="ACG8ZM_002691"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="ACG8ZM_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0493602.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKRAQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="ACG8ZM_002692"
     CDS             complement(2017..2919)
                     /locus_tag="ACG8ZM_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0493603.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="ACG8ZM_002693"
     CDS             complement(2916..3608)
                     /locus_tag="ACG8ZM_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0493604.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="ACG8ZM_002694"
     CDS             complement(3651..4292)
                     /locus_tag="ACG8ZM_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0493605.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
BASE COUNT         1376 a          949 c          768 g         1357 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtgc acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc
//
LOCUS       DAZKOR010000144         4385 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000144 DAZKOR010000000
VERSION     DAZKOR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.150"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..1347
                     /locus_tag="ACG8ZM_002695"
     CDS             13..1347
                     /locus_tag="ACG8ZM_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0493606.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            1388..2299
                     /locus_tag="ACG8ZM_002696"
     CDS             1388..2299
                     /locus_tag="ACG8ZM_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0493607.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            2296..3150
                     /locus_tag="ACG8ZM_002697"
     CDS             2296..3150
                     /locus_tag="ACG8ZM_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0493608.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            3147..3764
                     /locus_tag="ACG8ZM_002698"
     CDS             3147..3764
                     /locus_tag="ACG8ZM_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0493609.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            3766..4284
                     /locus_tag="ACG8ZM_002699"
     CDS             3766..4284
                     /locus_tag="ACG8ZM_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0493610.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1308 a          752 c          893 g         1432 t
ORIGIN      
        1 tggaggtaaa atatgaaaaa attattgttt acagggttgc tggcaagttc agcattgttt
       61 ttattaggag gatgcggatc aagtaatgca agcgatgcca gttcaagtgc agcaaattct
      121 tctgaaaaaa caagtgatgg aagaaaaaat attgatttct ggtccttttg gggatccggt
      181 gcacgaaaag aagctatcga agaaatcatt gaggatttca atcaatcaca aaacgagatc
      241 catgtaaatt ataagtatca gccatggggt gacatctgga ctaaatcttt atctgcaata
      301 acagctggaa atgcacccga tgttattgta caggacataa attccgttgc ccaaagagca
      361 gaagcacagc aagcaactaa tctctccaaa tatgtagaaa aagacacttc taaggatttt
      421 tatccgcagc tttgggatac agtagaatat gaaggtgatc cttatgcgat cccattcaat
      481 acagatacac aagtcatttt ttataataaa aagttattta aagaagctgg aatcagtgaa
      541 caagatctac cgacaacttg ggatgaatta gaagaaacag cacgtaaact agataaaaaa
      601 gaaggaaaag aatttacacg catcggtttt tatccattat ggaatttagg ggctgatgta
      661 tgggcgctaa acgcagataa cgggactagt tggtttgaca aagatgaaaa agtcaagatt
      721 gatactaaaa ataaagtgga agccttggag tggatccaag actggcaaga ttattatggg
      781 aaaaatacga tcaataaatt agaagccgaa tttgggtctg gtgtatctga tccatttatt
      841 tccggattag tagccatgcg tgcccaaaat atcaattatt actctagttt gatggaaaat
      901 gcaccggatg attttgattt tggcgtgatg cagctcccag aaaaagaaga aggttcaggc
      961 cattggtcgt ggggtggcgg attcgtcttg gaagtaccat acggagcaaa agatcctgag
     1021 gcctcttatg aattcatcaa atatctttct tctccagaag tacaagaaaa attcggcgaa
     1081 aaaagttttg atatcatggc gagcaagaca gccaatgaga atttagtgaa taatgacaac
     1141 ctaacggata aaggaaaaat gatctatcaa atggcggacg aaaactttaa aaatacggtg
     1201 atcacaccag ttcctttaga tgctccggat tacacgacat tagtgaatga acagattgac
     1261 cagattcttt taggaacaaa atcaccaaaa gaaggattgg cagatgcaca aaaagcagta
     1321 gaaaacttag tcaagcaaaa tcaataatcc atttttgact gtctaggtac agaggaggca
     1381 agaaaatttg aagcgtttat ctatccgaaa gaaaaaagaa gcaagatggg cctatttatt
     1441 tatctcgcct tttattatcg ggttcttggt atttatgcta gggcctatgc ttttttcagt
     1501 tattggtagt ttcaccgatt ataatttgac atccaagatg aatttcattg gtctagcaaa
     1561 tttcaaaaga atgttcgttc acgatgattt attttggaaa tcgttataca acaccgttta
     1621 tttcgtcatc ttcaatgtac cgctgacgac gattttcagt gtgtttttag ccacagtact
     1681 caatcaaaaa atcaaaggaa tcagtgtatt ccgtacagct ttctatttac cggcagtctt
     1741 atcaggagta gcagtctaca tcttatggat gcagctgctt tctccatctg caggattgat
     1801 caacacattt ctgggctggt tccatatccc tggaccagct tggctgtttg atccacaatg
     1861 gacgaaacct gcgttgattt tgatgaaaat atggagttct ggaggagcaa tgctacttta
     1921 cttagctaca ttacaaaatg ttcctagatc tttatatgaa tcagcagaaa tcgatggagc
     1981 aggcgtttgg ggaaagttca aaagtattac tttgccatta atcactccta ttatttttta
     2041 cgatgtgatc acctctacga tcggttcttt ccaaattttt caagaagctt atgtcatgac
     2101 gaaaaatgga acaggcggac ctgcaaattc tttgcttttt tataatctgc atatgtggaa
     2161 caaggcgttc gtagcttttg atatgggtta tgcgatggct atgtcaatga tcctgtttct
     2221 gatcgtgttg gttctgacgt ttgtcaattt gaagcttgca cctaagtggg tctattattc
     2281 tggaggtgac ggcaaatgat ggattatttc aaaaagacca gtgtgaatcg gcaaacaaaa
     2341 aaaatactaa gttatgtcct catgacagta ataggaatca ttttgatcat tccattgctt
     2401 tggatggtgt ttacttcatt gaaaccaatg gaagaaatcg ttcgttatcc tccgaccttt
     2461 ttcccagaaa aaatcgtatg ggaaaactat ctggatacga ttgccgcctt tcctttttgg
     2521 cgctatgcaa gaaatacatt attcatcact gtgctagtag tgatcggtaa tgtcctttca
     2581 aattctttta ttgcttatgg atttgcgaag cttgattttc ctgggaaaaa gctgatgttt
     2641 gctttggttc tttctacgat gatgatcccg gggtttgtaa cgatgatccc tcaatatgta
     2701 ttattctcca agatcggctg ggtaggtact tatttgcccc tgatcgttcc atcgttcttc
     2761 ggcaatgcgt tcaatatatt tctgatgcgt caattttact tatcgatcaa taatgaattg
     2821 atcgaagctg ctgaaatcga cggagcgaat catttgtata tttggagtca tttgatgctt
     2881 ccattgacga aacctgcctt gattaccatt gcgatcaatt ccttcaacgc agcttggaat
     2941 gatttcttag gtcctctgtt atatatccaa gatcaagaaa aatacacgtt acagattggc
     3001 ttgcaagttt ttcagaacca agcaacgaca caatggaatt atctgatggc aggagcaaca
     3061 cttgtcttga tcccgacgat tttgctgttc ttttttgcac aacgttattt cattgaaggg
     3121 atggatttga caggaggttc aaaaggatga tgaatcgatc gattggtaaa aaagccaatg
     3181 attatttctt agaaatcatt gaatggattc cacgtcttat ccatttacag ctactatggt
     3241 tcctgtgcgt cttgcctgtt ttcacattag gaactgcaag tcgaacagtt ctgtatatga
     3301 tttatcatta caaaaaaaat gaagagaaaa agctcggttc actattctgg aaaaagtttc
     3361 gtgagaactt ctctctttat ggaaagcaag atagtttagc aagtgtttat tttcttcttc
     3421 tattgatcga ttatcagatt ttccgctatt ggggtggggg aattggctat acattgatgt
     3481 atactacgtt gtttttgctt ctattaagcg cgcttctctt tagttacaaa atcttgcttc
     3541 aactggaaca aaaagaagct tcttggatta ctgcattttt cttgtttttt aatgatttca
     3601 aaagcgcgct tttctatttg gcaggcacgt tgatccttct tgtcgcattc tggtttgcag
     3661 gaccaatcta ttttgcttta ttaggtttca gtttcttgtt ttattttcaa gtcctgcttt
     3721 ttatccatcg tacgcaagaa aaggttctta aaaaggagga ataacatgtt gcgtttgatt
     3781 gctaaaatcg atgatagtat tatcaacaag ttcgccaagc aacggaaaac ggatagtcga
     3841 aaaatcaatt ttttgaaatt tatcagtaat atctctaatg gtggttttat ttggttgctc
     3901 cttgctgctg ttttagtctt gatacctgat actagagaat atggtctagc cattcttatc
     3961 ggcagtttga tctatttttt tgttggtttg ctttttatca aaaaaacgac aagacggaaa
     4021 agaccttatg aaaaaaatcg tcggcttgtc ctttacacaa gtaaaccgaa gacttcttct
     4081 tttccttcag gacatacagc gctagccttt ttatcagtag ctgcgttgtt cgaattaagt
     4141 ccagttgcag gaatattagc ctttttattt gcttgtttga tcgcttattc gaggattttt
     4201 ttacttttac attatcctac tgacatttta ggaggagcta ttttaggaag tgttattggc
     4261 atcctatgcg ctgccgttat ctgaattaat tgtacgaata aagcatgagt gcgtaaatgc
     4321 gcatttgtgc tttatttttt tgtttcgagg aaagcgtagt tgtttattca tttgtcttaa
     4381 taact
//
LOCUS       DAZKOR010000145         4282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000145 DAZKOR010000000
VERSION     DAZKOR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.151"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..513
                     /locus_tag="ACG8ZM_002700"
     CDS             76..513
                     /locus_tag="ACG8ZM_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493611.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            608..1492
                     /locus_tag="ACG8ZM_002701"
     CDS             608..1492
                     /locus_tag="ACG8ZM_002701"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0493612.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1714..>4282
                     /locus_tag="ACG8ZM_002702"
     CDS             1714..>4282
                     /locus_tag="ACG8ZM_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0493613.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     "
BASE COUNT         1403 a          720 c          963 g         1196 t
ORIGIN      
        1 agccacccta gatatttttc tgtataatct taaataaaga aaacgatatt aaaaaatgca
       61 cgtagaagta gataaatgga gtggttttgc aaatttcttt tatttctaga agttattcac
      121 gtatacggat ttgtttttct cacaaataaa aaggcaggaa gtacaatgga tcatttgtat
      181 ataaaatata ttcatcaatt ttccgttttg gtattaggca cactaattat agcggggatt
      241 gttgcattta ttattcaagg tcgtgacata ttaacagtac atcaagagtt tttcagttat
      301 gcatttggta ttcctctgat cgctataagg tctgataaca aaaagtggaa gaaacgctat
      361 tacattctct tgctattttt gtgtttttat ctgccaccta agatttttac tttattaggt
      421 atacaatgcc tatcattttc ttttatttcc tatatcattg ttttaatttt cctcagtttc
      481 atagtatggg tattttatct tgtcaaaaaa taaatgaatg tttcatgaac agtacttcat
      541 cacagcctaa tttttttctt tatgctatcg ttatattaaa gaaatcaatc agaagggaaa
      601 gaaagtgatg aaaaaagttt tattagtggt aaaccccagt tcaggcggtg aacaagcaaa
      661 ggaatttgag caattagcga tagcaaaatt ggaatcggtg tttgatgaag tggtcgtctt
      721 gcatacaaaa aaagcagggg atgcaaaaaa ctttactcgc gaagcggcta cggaagggta
      781 tcatagtgtg tttgtcatgg gcggagatgg aacagtcaac gaaggaatca gtggaatcgc
      841 tgaacaagaa catcggccaa atttcggatt tttcccattg ggtacggtga atgacttggc
      901 tcgagcatta gggataccgc tagaacctga ggaagcgatc aatcattttt cgattgaatc
      961 agtcaagcca ttggatattg gaaaaataaa tgacgactat tttatgaatg tcgtcgcaat
     1021 tggctctatc cctgaagcaa tcaatgatgt agatgcggag aagaagacga aatttggaaa
     1081 actagcttat ttcatgtcag gaatcaaaca gttagcttcc acacaatcct actcgtttca
     1141 cgtagaagtt gatggaaaga aggaagaaat tgagagcagt atcttgctga taggactaac
     1201 taactcagta ggcggttttg aaacattact tccaaatgca aaagtggatg atggtaagct
     1261 ccatttggtt tatttaaaag attcttcttt gatcgatacg cttaaaacat tgccagacct
     1321 attaaaagga gtagatgaat ctacgaaaaa tctagcctat cttacttgtg agcaattagt
     1381 tgtgtcatta gctgatgagg aagaattgac gacgaatgtt gatggagatg aaggggataa
     1441 attacctgtg acaatcaaag tgttaccctc acatttacag gtttattgtt aaaatggagg
     1501 ttgtgaccga agagttgtca ggaggacatc ggttgttcgg aataagggac tgtgacaaga
     1561 cttttgtcac agcctttttt tgtacaaaaa agaaattttc acctaacaga aaaatttgcc
     1621 ataaaaatac atattttaaa tatgaaagta caattacaaa taaattaaaa cgttaaaata
     1681 aaattgtcaa aacgtcagag aggatggtgg agaatgaatc aatatgtaaa aaagctgatt
     1741 gcgtcgattt tgtttgtctt ccctctcttg atggtgacat ccgtttttgc cgatgtagag
     1801 aaaacaagcg aatcaacgca atcagagaat caaagttttg acaagactac tttttcagaa
     1861 gaatcaatta cttctgaaac tacagaatcc caaagtgagg cgcctacttc taatacaagg
     1921 caagaagaaa ataagacaag tgagacctca gaagtagctg aacaaacgtc tgatagcagt
     1981 gaagaggtac aggttagagt tgtttcagcg gatgggtaca gcgaagctgc cacaacagaa
     2041 gaactgacgc agcttctcgc agacgaatca gtagctaaga ttcgtttaac tcagcctttg
     2101 actttagaca gggaattgga gatcaagcga gatatcgtta tcgattttgg cggacttgct
     2161 cataatttcg gcacacatca catctatatc aatgagacac ctcagtcgat cgagtttcag
     2221 aattttaaag gaacggcagc tcatccaggt gggttgatac caaatatcga tggaaatgcc
     2281 attatcattg cttatatgtc cgatttttgg ggaaacagat atcattttac gggagaaata
     2341 aaatttaccg ggacacttga cttatatgat ggttctaaat taggcctgat ttacgcccct
     2401 cgtgcaactg tcacattaga tggtgtttcc ggtgtattgg atgtccaacc agtgaaagaa
     2461 gggatgaatg ccgctccggg taaagcttat ttttgtagaa gctatcagct aaatgtaatc
     2521 aatggtagtc agctttatgg accatatcta ggaaaattct atggttttct agattcaggt
     2581 gatgagacgg gcagtggaaa aacagcacca ggactccata tcttgagtgg ttcaaaggtc
     2641 tctcttgact atgatcgggc agatggcgtc agcgatgaag gagaggctgt cgatacgcta
     2701 cccgggaatg tcacatttaa agtcagtggc tcaggaagtg aattttcggt aaatacaaag
     2761 atcaatatta ccaacgacaa caatcgcgga attatccaaa tgagaggctc aggatcaaga
     2821 gtcgctgtgt cgaatgatgg gaaaatcaca atcaatacag cgactactgg cggatttcgc
     2881 ctgcaaggag attctagtca aatccatgta tcaagtggtg gacaaatctc ggtcaaaatg
     2941 gctggagatg gcgaccaacc tggaaacaat gggatgcgtt ttgtagggaa aggactttct
     3001 ttgatcgtag aaggagccgg atcagtcatc gatattgata agcaaagtgg tcgttctagc
     3061 gcagttcgtt ttgagaacgg tacgcaaaaa ctaacagtaa ctaatggagg ctcattgaag
     3121 gtaagaaatg caggggatgg caaatcttat gtgaacagac tcaatcaagg aaaccaagcg
     3181 atccaatttt atgacgcttc tggttttcta gaaagtccag ggtcagctga ttttacggtt
     3241 actggagaaa aatcaaatat cgatatacgc gcagattccg gagctaccgt agatacaact
     3301 ggagatattg atttgaactt tatggctggc gaaaaaactt atttagtcat ggtcgggaaa
     3361 actggggaag ataagactgg catcatcagt ggggatgtgt tgaatgtaga cttagaatct
     3421 cctacctatt tcgatttcca aaacaaacgg atcggaagcg agactaatgg cataggtggc
     3481 tgggtatttc aaggaaatgg cgattcttct ttgaagatcg ctcattcaga aattgcttta
     3541 tggcgaaagg caggagtaac gttcgataac tttgaaaaag atccggatta tttttctgat
     3601 atgacagact tatcgatcaa tggaaccgac ttaggtaatt ttgtatcaag ccctagtgca
     3661 ggtttaacaa atgaatttgc tcagacaggt gccggaatga aaaattataa tcgaatcagt
     3721 gccaataacc agcttccgat gattgaatcc ttaagagtac caacgaatgc agataaaaaa
     3781 atctatgggc atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga tgcttggaca
     3841 ggcgaagtag aagtgacttt acgtattact tatgcagata cgacgaaatc acctgtagaa
     3901 attactgctc tcacacaagg acaaacagat gaaaatgaag ggacaggata taatccgtgg
     3961 ggagaaggcg aacaaggcgg tttgtttgag gttcaagtac ccaacgaaga aagactaaca
     4021 acgggggatc aagtgcaaat agttgctgct aaaaaagtaa gaggcggcca agaagtgaca
     4081 gaactggact ctgccaaaac gacagtcgat gtgattcctc ctgagcctgc taaattaagt
     4141 accacattga tcaatgcagc ttctcgctca atcacaggta caggcgaagc aggagctttg
     4201 gtttcattga aaaaaaatca agctgttttg tccgaaacaa ctattgatga tacaggtaaa
     4261 tttgttcttt ctatcccaga aa
//
LOCUS       DAZKOR010000146         4167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000146 DAZKOR010000000
VERSION     DAZKOR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.152"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            313..633
                     /locus_tag="ACG8ZM_002703"
     CDS             313..633
                     /locus_tag="ACG8ZM_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0493614.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            651..1082
                     /locus_tag="ACG8ZM_002704"
     CDS             651..1082
                     /locus_tag="ACG8ZM_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0493615.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            1212..1673
                     /locus_tag="ACG8ZM_002705"
     CDS             1212..1673
                     /locus_tag="ACG8ZM_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0493616.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            1707..2342
                     /locus_tag="ACG8ZM_002706"
     CDS             1707..2342
                     /locus_tag="ACG8ZM_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0493617.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            2455..3090
                     /locus_tag="ACG8ZM_002707"
     CDS             2455..3090
                     /locus_tag="ACG8ZM_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493618.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            3389..>4167
                     /locus_tag="ACG8ZM_002708"
     CDS             3389..>4167
                     /locus_tag="ACG8ZM_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0493619.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLL"
BASE COUNT         1620 a          598 c          698 g         1251 t
ORIGIN      
        1 tgcggtgtgt tcatatttct cattcctttc ttttatattc gtaaacaaat ttaacaactt
       61 ttttctaaat tcaattgaca gtatctgagt aatactctat actaagagca taattaaata
      121 agacataaaa acattgattt taaagctttc ttggcggtct gcatttacat atcaatagtg
      181 ttttttgttg tctttttagt tgttaaatct gtttacaaga tatagtatag ctcattactc
      241 agaattagtc aaattatttc tgcgtatttt tctaaacttt ttttgtaaac atttagaaag
      301 gttgttttat cgatgaatac ctatgaaata ataaaagact tggcgaagag gaaaaagata
      361 tctatacggc agttagaaat taattttgga tattctaacg gttatctagc aaagtggaaa
      421 aataatactc ctaattcaga tgaattaagt cggattgctg actatttcga tgtctctgta
      481 gactaccttc taggtagaga agaaagagaa acccctaaac atgtggattt atcagaagac
      541 gatactgttt tttcttttga tggaaaagaa atatctaagg agacaatgcg taaagcgatt
      601 gcaattgcta aagctttaga ggaaaatgaa tagttggagt gatggtttgt atgtatttga
      661 aattgaaaga aatgttgagc gaatacaatt tgaaagtagt ttatatggag atgaaagagc
      721 ctggtttcta ctatccaaaa cccagaatca tttttttgaa tgaaaatcta tacggtgaaa
      781 ctgcagaagc ctttcattta tctcatgaac ttgcacattt cagcgcctct cattttgaat
      841 tttcagtttt gtacgatact tctacaactt ttcactcaaa atttgaaaca gaagcagata
      901 aaatagctat tctaatttta ttaaatatct atattgagaa cgaactgact gatgaatctc
      961 agtttaatct cgaaaaattt atggagtatt attctattca gaataagctc agatacactt
     1021 gttatgctgt ctgccaatgc tattttaaga aaaaatattc ttacgcaagg caatatgtat
     1081 gaatacatca agaattatta aaaaaaccga tttcagcaaa acattaaaaa agcccgtgct
     1141 gcaacacggg ctctttcctc atatatgagc ttctacaaaa aaaatcatat cacagaaatg
     1201 aggaagaaaa aatggaaaaa gagattttaa taaaatctcc aggaaaaacc ttgattaaag
     1261 taactgatga ttctatttca attattagaa aaggatttat aaatttagtt aatcaaggta
     1321 ttaaaggaga aaaaactatc ccctttaaaa atatatcagc cgtacaatta aaaaaaccag
     1381 gtatgagtaa tggttatata caatttacct tgctaggcgg aaacgaaagt cgcggaggta
     1441 tattagcggc cacgaaagat gaaaacaccg ttatgtttac aaaaaaatat tggaatgaaa
     1501 tggaaaattt aaaaaaatat atagagaagc aacaatctat attagataat aattccaaag
     1561 aaacacaaat ttcaagtgca gatgaaatta aaaagtataa agagttgtta gatgaaggga
     1621 ttattaatca agaagaattc gattttaaga aaaaagaatt gcttggttta taatattcaa
     1681 tatcaaaaga gaaagaggat ctattaatgg acggtatatt tggttttact ggtattgttt
     1741 tatttattat aggtttaatc atgttaattg taagatttat caaaaaaaca aataagaaaa
     1801 cacctatgat tctccttatt atcgggatta tttttactac cgtaggtttc tcgctttcct
     1861 catcaaacga ggcaaatgac aataacaaac aggaaagctc atcatctgat actcaaacta
     1921 ctacatctaa cagcagcaat aaaagcctaa ctcaatttga agaattttta gagacaaata
     1981 atcatgactg gggtgctttc ttagatagtt attattctat tactccagct actgagcaaa
     2041 atacagcttt tactaaatat attagtggta aaacatatac ttttgaagga actgttatcg
     2101 aatcaatgac tactagaata gccattatag cagacaaaga gtacgatgat aaaagttgga
     2161 atgatatttc tgcaacacct aaagtttcat atgtaatttt tgcgaaggac ttaaacaatg
     2221 ctgatacctt tacaaagggc gataaagtaa cttttacagg agaaattagt tcaagaggat
     2281 cgaacataga aaaatcctat gctcaatggg atatgattaa tagtaaagta agtaaaaaat
     2341 aaatgaacca cgccccacta agagttagat tgtgcacaat aataaaacct atacaaaaga
     2401 gtgccatcag gagtcacaat gcttgtaaag gaaatgacaa aataggaggt aactatggat
     2461 aatagcaatg aaaaactaac caaaaaagaa tatcttgaat taatagttga aggcggtatt
     2521 ggagcaattc cttatattgg tggcgttgtg cagtcacttt attttgggtc taaaaacgag
     2581 aagcgtttca aacgcatcga gaaattctac aaaaatttaa acgaatcatt agaaaacatt
     2641 cgcgatcaaa ttccagaaga tgttttcaac ttagagaaca aggaccaact cattggaata
     2701 ttcgaaggga taaatgacga ggtagaaaaa tcaaaggcac aaaataaaat agatatgtat
     2761 aaaaatcttt ataagaattg tttacttcga attaacaacg cttcatggga taacgaggag
     2821 tattatcttc ttgtactgaa tcaattaaca tcgattgaga ttcaattgtt agcatgctta
     2881 atgagtcgtg gaaacgaaaa tttcactgga aatatcagca tgtctggcta ttcacaagag
     2941 ctaatagatg gtagcttaaa tagattatct gattttggtc tgttagaaaa aacgattagc
     3001 tccatagtat taggtgatgt cggcaaacaa aatatgggct acaaaatatc tcatttagga
     3061 attcaattta ttaactcaac tcttacttaa gttttctttc taatatgaca tctagaccta
     3121 tcagcaatgc aacaataaaa aaccagacat accaataatc aacaaataat ctaaactctg
     3181 gaagttcagt tattctttta aaaatcactt taatcataat aaacacctct gattggatga
     3241 tctaatacac atccaattat accattaaaa cgacaaatag cacatccttg cgccggcaag
     3301 caaaaaggat gtgcaaaaaa taataaaacc caaacaatgg gcttctttat agttcctatt
     3361 gtatcagaga aagagagctg attcaattat ggcaagatta gtcaaacgtg gaaatagttg
     3421 gcaatacgaa atttcataca aaaaagatga cggaaaatac acgaagataa gaaaatcagg
     3481 atttaagaca aaaggagaag caaaagatgc cgccaacgaa ttagaatata acttgaacaa
     3541 aggccttaaa ggggatcgca aaaatctatt attatcagat tactttgagg attggatgca
     3601 actttataaa gaaggaacag tatctcctat cacttataga aaatacgaag atacgttaat
     3661 gaacataaag aaatatatgc cagcggtatt gatttctgat ttagatagag tgggatatca
     3721 acgcttttta aataagtatg ctaaagacca tgtaaaatct accgttatta agtttaataa
     3781 ccatattaga gcgtcgttga aagatgccgt agaagaagga ttaattccgt ttgatccaac
     3841 tagaaaagca gtaatcaaag gaaaagattc attgaagcca aaagaagata aatatttaga
     3901 ttatgatcaa tttaaatctt taatgaaact cgtagaagaa aacctttctg cgcagtactc
     3961 ttctcctatg ctcgtgttag ttgctggtgc cactggaatg cgatttgctg aacttctagg
     4021 attaacatgg gaagatatcg atttcgaaga tcaaatcatc actattaata aaacatggaa
     4081 ttataaatta aatgaatggg gaaaaacaaa aaacgaaact tcaaatagga aaatttccat
     4141 tgataaacat acgattgatc tcttaaa
//
LOCUS       DAZKOR010000147         4152 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000147 DAZKOR010000000
VERSION     DAZKOR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.153"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..106
                     /locus_tag="ACG8ZM_002709"
     CDS             <1..106
                     /locus_tag="ACG8ZM_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0493620.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="ACG8ZM_002710"
     CDS             complement(359..508)
                     /locus_tag="ACG8ZM_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493621.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            970..1182
                     /locus_tag="ACG8ZM_002711"
     CDS             970..1182
                     /locus_tag="ACG8ZM_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493622.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="ACG8ZM_002712"
     CDS             complement(1460..3259)
                     /locus_tag="ACG8ZM_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0493623.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="ACG8ZM_002713"
     CDS             complement(3383..3979)
                     /locus_tag="ACG8ZM_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0493624.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1227 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt tt
//
LOCUS       DAZKOR010000148         4097 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000148 DAZKOR010000000
VERSION     DAZKOR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.154"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..751
                     /locus_tag="ACG8ZM_002714"
     CDS             <1..751
                     /locus_tag="ACG8ZM_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0493625.1"
                     /translation="FNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEHAVKLR
                     KEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQEWNAQI
                     QKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHVDYFEP
                     CFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGSSFVYP
                     RTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            738..2179
                     /locus_tag="ACG8ZM_002715"
                     /pseudo
     CDS             738..2179
                     /locus_tag="ACG8ZM_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            2190..3521
                     /locus_tag="ACG8ZM_002716"
     CDS             2190..3521
                     /locus_tag="ACG8ZM_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0493626.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            3534..3992
                     /locus_tag="ACG8ZM_002717"
     CDS             3534..3992
                     /locus_tag="ACG8ZM_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0493627.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1367 a          605 c          878 g         1247 t
ORIGIN      
        1 ttttaattcg gatgattttg gctatagcca tggtgtgaat tatgggatta tggatgctta
       61 tcagcgagga attttgacat caactacatt gatggcaaat atgccgggtt tcgagcatgc
      121 tgtgaaacta agaaaggaaa tgcctaggct aggtgtagga gtccatttaa cattgacgtg
      181 tggcaaacca ctcctcaaaa ctgtcgatac tcttatcgaa agcggagagt tcagatcctt
      241 atcttattat cagcatccat ttaacattga caaagatcaa ctgtatcagg aatggaatgc
      301 tcagattcaa aaaatttatc atgcggggat cataccaact catttagaca gtcatcatca
      361 tactcatact ttggatgaaa atcaggaggt agttgttaca ttagcaaaaa aatatgattt
      421 gccggttcgg agcaactttg aacgaaaaga tgaagttcgt catgtcgatt attttgagcc
      481 ttgtttcgat gtcgtaggag aaactgatga aaaaaaacga caaatcaata cgagtgtgga
      541 agaatatttg gcaagtttac tcatgatatt gagagaagat aaaacgatcg aagtcatgtg
      601 tcacgcagcg tatttggatc aggaattgtt aaatggttca agttttgtgt atcctcgtac
      661 ccaccaagtc gaatttttaa ttcattcagc atttgctcaa aaagtgaaaa atgatccttt
      721 gattgagtta gtaacatatg aggatattta atagaggagg aataaaaatg aatgttaaat
      781 caaatgtgca gaagcttgga agagcgatgc tacttccagt tgcagcgatg ccattagcgg
      841 gattgatcat gcgtttatca gctgacgata tgctgaacat cccagtaatt ggtgcagctg
      901 gaaatgccgt ttttggaaac ttggatatct tatttgctat aggagttgca atcggttttg
      961 tcaaaaataa ggacaaagga atacctgcac ttactggttt tttagcgatt gctaccttga
     1021 aaaaaggctt agaaattatg gacccagaag tgaatatggg gatttttggt ggaattattt
     1081 ccggtttagt agcggcatgg acgtataatc gctttaaaga acaaaaatta ccagtagttt
     1141 tttcttattt tgcgggcgaa aaattcccat tgactatggt gatgattcta caaacaatcg
     1201 cagctgtttt tttgggcttt atctggccgc tcatccaaat tgggattgat aattttgcaa
     1261 aactgctcgt tgatatggga gcattaggtg tggggatatt tatgtttttg aatcgtttgc
     1321 tgatcccctt tggactgcac catgtgttga atacgtatat ttattacgat ttaggaagct
     1381 atacatctcc tagcaaggaa gttttcagag gagagatgac acgctttatc aacggagatc
     1441 ctcaggctgg actgttttta tcagtttttt tgtagtaatg atgtttggta taccagctat
     1501 atgtctagct atctataaag cagcttataa agaaaataaa gagatggtca aaggaatcat
     1561 gggaagcggt gcaggaactt catttattgc aaatatcact gagcctgttg aatttagttt
     1621 catgtttctt tctcctatgt tgtacgtgat ccatgcactt tttgccggtt tagcagggat
     1681 tgtctgttac ttattcaata tacgaattgg ttttacattc ggagcatgta ttgttgatta
     1741 tctcatcaat tttcgaatcg ctaccaatgc tattttgatt cttccaatcg gaattttctt
     1801 ttttgccttg tattacgtta ctttttatta tttgatcaat aagagaaaca ttcagacatt
     1861 aggacgtgaa gctaaggcag agtttggcaa tgaagtaacc ttagaagaaa cagagttagg
     1921 gttagcaagt aaaaattatg aatatatggc aacaaaaatg ctacaagcgt ttggaggaaa
     1981 agccaatatc ctggatgtct atagttgtaa tacacgccta cgggtagagg tagtggatcc
     2041 caccatggtc gaggaacagc gaatcaagca attagggata agcgggatca ttaaaccaac
     2101 tgaaaaaaat tatcaaatta tcatcggctt agaagtgact tatgttatgg cagaattcaa
     2161 taaattatta gaagaataga aaggaaaaga tgatgatgaa aaaacagatt atcactatag
     2221 ctggaggcgg gagtacgtat acaccaggaa tcgttcaagc cattttgaac aatgaaaaac
     2281 ggttgccatt atctgaaata cgtttatatg atattgatga agaaagaaat atggatatgt
     2341 acttgattgt taaatttatg ctgaaaagaa aaggtttttc aaatattcgc attcgtgcaa
     2401 cggatgatcc aaagcttgct tttacaggat gcgattttgt tttctcacaa attcgtgttg
     2461 gaggattgga gatgcgagaa aaggacgaaa agattccact taaacatgga ttagtaggac
     2521 aagaaacgtg tggactcgga ggatttgctt atgggatgcg ctcaatcaaa ggtttattgg
     2581 aaattgtcga tcatatacaa gactatgccc ccaaagcatg gattttgaat tatacaaatc
     2641 cagagtcgat cgtttctgaa gctgttcgtc gtaaatatcc gaatgtaaaa atgattaatg
     2701 cctgtgatat gactatttca attgaagaaa cgattgcagt gaattacggt tatgatcgta
     2761 agaattggat cgttacgtat tatggtttaa accactttgg ctggtataca tcgatttatg
     2821 ataaagaatt gcaacgagaa attatgccag agattattga aaaactaata acaaaagaaa
     2881 tgcaagtcgc cgattttaat ataggagata aaacttggca aaaaaccttt caaatgatgt
     2941 cagtcatcac gaaaaatttt ccaagtaata tcccgaataa ttacttagag tattacttat
     3001 atcctgatat ggtcgttgag catactgaca aagagtatac acgtgctaat atggtcatgg
     3061 atggacgaga gaaaaatacc aaagaaatgg ctgataaaat acgccgcggt attgtggaag
     3121 aagtattgaa ctttaatttt ggggagcatg gacaatatat cgtcgatata gctatttcct
     3181 tgttgaatga tgatcgtcgt cgctttatgc taatcgttcc taatcaaggg gcgattccga
     3241 atctacggtc agatgcagtt gttgaaatac cggcatatgt tggtgcaact ggtgtggagc
     3301 caattacact aagaaaacca atcagtgatt ttcataaagg attgatggag gcacaagttg
     3361 cagtagagaa gttactagta gatgcttact ttgaaggttc ttatcaaaaa gctttacaag
     3421 catttacatt gaatcaaacc gtaccaaatg ccagagtcgc aaaaaaaata ctagatgaaa
     3481 tgatcaaagt aaacaaaaaa tattggccag cattaaagta gaggtgataa aaagtgcatt
     3541 tctttaaaac aagaaacatt ctaaaagctg tagctagtgg tcaaattatt ccattaacag
     3601 cagtaaaaga cgaggtcttt tcttcaaaaa tgataggagt tggatacgct ataacgaatc
     3661 atgatggata tatccatgcg ccagttaaag gaataatcga taatatattt cctacccgac
     3721 atgccataac gatcgaaagt gaatctggca gaaaaatact gatccatatg ggtttagata
     3781 cagttgaact cgaaggaagt ccgtttcaga tacgggttga aagtaaccag gaggtaaaag
     3841 aagggacact gttagctatt atagatttga agaagttgga gcaagcacaa aaagatacgg
     3901 tgatcattgt agtttctcca ggcatttttg aaggaaattt attaaaacaa aaccaagaag
     3961 taaagattaa agatagttgt tttgaatttt agtgtacgat gaaaaaagtt aaatagggtg
     4021 atatttatgg gtgaatttac aaaatttttt tgaaatgata tgtttcaatc actagattta
     4081 atcattttga tttttgt
//
LOCUS       DAZKOR010000149         4070 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000149 DAZKOR010000000
VERSION     DAZKOR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.155"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1435
                     /locus_tag="ACG8ZM_002718"
     CDS             86..1435
                     /locus_tag="ACG8ZM_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0493628.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="ACG8ZM_002719"
     CDS             complement(1563..2153)
                     /locus_tag="ACG8ZM_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0493629.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="ACG8ZM_002720"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="ACG8ZM_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0493630.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1176 a          744 c          789 g         1361 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaaaaaa
//
LOCUS       DAZKOR010000150         4005 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000150 DAZKOR010000000
VERSION     DAZKOR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.156"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..617
                     /locus_tag="ACG8ZM_002721"
     CDS             <1..617
                     /locus_tag="ACG8ZM_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0493631.1"
                     /translation="NLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLG
                     MDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQ
                     FIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNH
                     TISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            714..1067
                     /locus_tag="ACG8ZM_002722"
     CDS             714..1067
                     /locus_tag="ACG8ZM_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493632.1"
                     /translation="MSCFVESERLSNGNNSVLNEYDKVVINKVKSLCEEARESISHIN
                     GSNFKQTLFNLIKLDAKISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKL
                     NNYKLIDYKYLSESN"
     gene            1684..>4005
                     /gene="esp"
                     /locus_tag="ACG8ZM_002723"
     CDS             1684..>4005
                     /gene="esp"
                     /locus_tag="ACG8ZM_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFQ0493633.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFT"
BASE COUNT         1462 a          556 c          763 g         1224 t
ORIGIN      
        1 gaaatttaga aaacatggtt tttaatctga gtaatggaat aattccaagt gttagtggag
       61 atacgattcg gtctgaaaaa aattattcta tcattgtttt tgaaaagtta gcacaaacgt
      121 ctataacatt aggaatggat ataatagaag cgtatcaaag tagagatgct ttgatacaag
      181 aaaacgaatt agctgtatcg ttacctgagg tcttaaaagt gagggattct gggattgttt
      241 attatactaa ggaaatcgga aaaactaaga tagagcatct ttctccctta atttcttctg
      301 tcgttcaatt catcggctta aatatctata aaagaattac tgtaaaggaa atagctaatt
      361 atttttctgt aagtgaaact aaattaagag aaagctttaa gaatgaaatg catattacga
      421 tttataatta tatctctaag cgaaaaattt ctacggctaa aataatgttg aaatcaaacc
      481 atactatcag tgaagtttct ttaggtttag gattttcaga ttcttcacac ttttcgagag
      541 tattcaaaaa gtacgcaggt gtatctccta agcaatatca attaggttta gtggataatt
      601 tcaacaacat tagctgagag gttttatttt attatgagac tatatcattt acgtagtttg
      661 gaggagttga ggaaattgga ttttaacaaa aaattttttg ggaaatttct gatttgagtt
      721 gtttcgtaga atctgagcgt ctaagtaatg gtaataatag tgtgttaaat gaatatgaca
      781 aagtagtaat taataaagta aaaagtttat gtgaagaagc tagagaatct atcagtcata
      841 ttaatggtag caattttaag caaacattat ttaatctgat taagctagat gctaaaatat
      901 caagttattt attcttttta atacatgatg aatttatgga ttatcaaaaa ttggatcagt
      961 taattgaaag agaaagctgg gaagattatt acgtgggtct aactttttca gaaaagttaa
     1021 acaactacaa gttaatagat tataagtatt tgagtgaaag caattaatta ttcatgtaga
     1081 gtactagaca ctttaaatat ggacaattca attataaaga aaaagaatca ataatttaat
     1141 gaggctgtga caaatgtcat tttttgtttc gctaaaaacg agtgaaaaaa tgttgataac
     1201 tccaatcatt cgtttaagat tctaggaatc attcaggaat gctgtgaaac cgatacttta
     1261 taattataaa gaaggtgatt tcaaattccg aagaaggaat ttgaagtcac ctttttactt
     1321 atgtcacagt ctcattcagt aatgcgggat aaggctcctt attccgctat ttgaatactc
     1381 tgaagtttaa tctaagaaag agtaatattt tgaaaacatg cttcatcgca tagaaatgaa
     1441 cttaatttca gaaagaatac atacatatct ttttatatgt tttcagctgc tgaatggtta
     1501 aaaagcacaa atatccgaaa aaaacatgaa gaaatgtact ttttttgttg taatgtgcat
     1561 gaaattcttt aagtaaacta gttaacataa gtttagtaag attcctattc tgtgaataat
     1621 acgtatcaga tttatctttt attcttacta agaaaataac tattaaggga gttgatttga
     1681 ataatggtta gcaagaataa taagagagta tttctagaaa aaacaaagaa acgagtattg
     1741 aagtacagta tcaaaaaatt aagtgttggg gttgcatctg ttttggttgg tgttggtctt
     1801 gtcttgggaa caactgaact agttcaagca caagatgaaa tttctccaag caccccactt
     1861 gaaacagcga taagctccgt acaagtaggt gacaaagtag cttcggggaa tacttttcag
     1921 gagaatcctg gttatacgaa aaattataac ttttcggatt tacaatttag tcctcaagaa
     1981 ctaactggag acacactaaa aggcaataca attggatttg aagtttacgg aaaacataat
     2041 attgcagcat caactaaaaa ctgggaaatc cgtcttcaat tagatgaacg attagctaaa
     2101 tatgtagaaa aaattcaggt cgatccaaag aaaggaattg gatcttctag acggaccttt
     2161 gtaagaatta atgattcgct gggtagacct acaaatattt ggaaggtcaa ttatattcgt
     2221 gcaagtgatg gattatttgc tggggctgaa acaactgata cacaaactgc tcctaatggg
     2281 gtaattacgt ttgaaaagag cttagatgaa atttttaaag agataggcat agacaatctc
     2341 aagaccgatc gtttaatgta tcgaatctac ttggtaagtc atcaagacga tgataaaatt
     2401 gtaccaggta ttgatagtac gggctacttt ttaacagatt cagatgattt ctataatagc
     2461 ttagatgtat ctgaaaataa tcctgaccag tttaaacatg gatcagtaag tgcaaaatat
     2521 gaggaaccta atactcaaac aaaagatggt tcaggatcca caggtgctaa tggagctata
     2581 atcttagatc ataagttaac gaaaaattat aatttctcat attcagcttc cgcaaagggc
     2641 acgccttggt atgctaatta caaaattgat gaacgattag ttccatatgt agcgggtata
     2701 cagatgcaca tggttcaggc tgacaaagta acatatgatg tttcttttga atcagggaaa
     2761 aaagtggctg atttagcaat tgaacggcgc aaagatcatg aaaattatgg tgtaggttca
     2821 attactgata atgatcttac caaacttatt gattttgcta atgcaagtcc acgtccagtc
     2881 gttattcgat atgttttaca attgactaag ccattagatg aaatcttaga agatatgaaa
     2941 gcaacagcac aagttgaaga aaataaacca tttggtgaag atttcatctt tgattcttgg
     3001 ttgtcggata cgaataaaaa attaatccag aacacttatg gaacaggtta ttattatttg
     3061 caagatattg atggtgatgg aaaccctgac gataaagaag agagcggaga cacgaatcca
     3121 tatatcggga aacctgaatt agaagaagta tatgatgttg acacaacagt taaggggaaa
     3181 gtattcatcc acgagttagc gggaacaggt cacaaagccc aacttgttga taaagaaggt
     3241 actgtattag cagaaaaaac tatcgctcca aatgaaaaag atggggctcc aatttcagat
     3301 actgtagaat ttgaatttac gggtgtagat tcaagtaaat taatcgcgaa agatgaatta
     3361 aaaatccaaa tcgtttctcc aggttttgat aaaccagaag aaggttcaac cgttattaag
     3421 gaatcaccaa aagcggttga taaacaaacc gtggtagttg gatttaaacc agatgctaaa
     3481 gaatcaattc ggaataataa aaacttacct gaagatgcag agtattcatg gaaaacagag
     3541 cctgatactt ctaacgttac tgatagtacg aaaggtattg taactgttaa gatcggaaat
     3601 cgaactttcg acgtggatgt agagtttgct gtaaaagctt ctcaagctat ggaaaatgat
     3661 gcaacatacg tacctataac aacaactcca gaaacgacag ttcaaagtgg aaaacctaca
     3721 tttgataaac cagatgttcc tttagctaaa gatgcctttt caattttaga tgtctataat
     3781 aaggacttcg gtaatgcaag tgttgatgca aatactggta ttgttacatt cactccagct
     3841 aaaggtgtag gagaatcgga gccgattact ggaacaattc ctattaaaat tgtttaccaa
     3901 gatggttctg taggcacgac cgatttagca gtaactgtaa gtaaagatat ttatgaaaat
     3961 ccaggagaaa acattcctgc aggctaccac aaagtgacct tcacc
//
LOCUS       DAZKOR010000151         3972 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000151 DAZKOR010000000
VERSION     DAZKOR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.157"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..713
                     /locus_tag="ACG8ZM_002724"
     CDS             27..713
                     /locus_tag="ACG8ZM_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0493634.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            715..1659
                     /locus_tag="ACG8ZM_002725"
     CDS             715..1659
                     /locus_tag="ACG8ZM_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0493635.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1858..2502
                     /gene="pcp"
                     /locus_tag="ACG8ZM_002726"
     CDS             1858..2502
                     /gene="pcp"
                     /locus_tag="ACG8ZM_002726"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0493636.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2581..3858
                     /locus_tag="ACG8ZM_002727"
     CDS             2581..3858
                     /locus_tag="ACG8ZM_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493637.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         1269 a          689 c          881 g         1133 t
ORIGIN      
        1 tattgtcatt ttatggagga aaatatatgg aaaattatct tagcctttta ggcgtcttga
       61 ttgttattct cgggtttgca tttaaattag actcgatatt gatcgtaatg gttgctttag
      121 ttgttacagc tttgactggc ggtcttggca ttcaaggaat gttagaactt ttaggtacaa
      181 gttttgtaaa caatcgcggg atggcgattt ttatcatcat tatgttggca acaggaacgc
      241 ttgaaagaaa tggtctgaaa gaatcagcgg cttccttgat caagcgattc aagaaagtat
      301 ctgctggtat gattgtagat atttatggga tctttcgaat gatctttgcg gcttttaacg
      361 tgagttttgg tggagttgct ggatttgttc gtccaatcat tttaccgatg gcattgggga
      421 ccatcgaatc aaaagggtta gaagtccatc ctaagcatga agaagaacta aaaggtatgg
      481 cttcagctat ggaaaatgtc tgctggttct ttggccaagt actattcatc ggaggagcag
      541 gggcgttgct agtccaatca acactgaaag atctaggata tgacgtaaca ttgggacagc
      601 ttgctttagt ggaagttcca gtggctatca cggctttagt tgtggcaagt gtctacttct
      661 atcttaaaga aaagaaatta gcgaagaaat attatccagg aaaggggcaa taagatggac
      721 ttttttacaa atcctgatat tttattaggc gataaattac tggagatcct ttatatcgtg
      781 atgggactcg tattgattta cactggagta agaaatttaa cagataaaac caatcctcat
      841 cgttatgggt cagccttttt ctggacggtc ttaggggtag ttattgcagg aggaagatgg
      901 cttccggcaa tcgtgaacgg tggcttgatc tttgcgatga ccataccagc aattgctaag
      961 aaagtcagca aaggagaaag ccggcttcca tcaaaagaat atatggaaaa aatgtcagac
     1021 aaactaggaa tgaaaatttt tattccagct ttgagtatcg gtgtctttgc gatcttgttt
     1081 gctctattta ccaatttagg tgctttagtc ggcgtgggcg tcggtgtttt tgcagccatg
     1141 ctaatcttga tgttcttttc tcgagataat cgaccaagta catttttaga tgatgcagca
     1201 gatatgctgg gcacagtagg gccgttaagc atgttgccaa tgcttttagc ttctctaggt
     1261 gcggtattta caagcgctgg agttgggaca gtgatttcca atgcagtagg tacgatcgtt
     1321 ccagaaggaa atgtgaacat cggcatcatc atttacgctc ttggcatggt gatatttacg
     1381 gtaatcatgg ggaatgcatt tgcggccatc accgtgatga cggttgggat cggtgcacca
     1441 ttcgtattta gtcatggagc gaatccagcg ttaataggaa tggttgcatt gacttgcggt
     1501 ttttgtggga ccctgctgac accgatggct gctaacttca atatcgtgcc tgtagcgatg
     1561 ctggagatga aagacaaata cggagtgatc aaaaaccagt tgtttattgc gttatttatg
     1621 ctagttttcc aaattgctta tatgattcta tttaaataat agaagagagg ggttgtgaca
     1681 gaataattgt cgcaacctct tctaacattg tagtggaaca ctattttttt ataagaatat
     1741 attaagtttg atctgttatg ttatttaaac tattgtttcc tttatttacg ctgttcaagg
     1801 aaaatggtat gataaaagat aaccatatgt taaatcaatc ggacggagga acaacagatg
     1861 aaagtactag taacaggttt tgatccattt ggtggggaca aagtgaatcc agcctatgaa
     1921 gcagtaaaaa aaatgccaga tgaaatcagc ggtgctgaaa tcatcaaagt ggaagtaccg
     1981 accgtattcg aaaaaagcag tcaagtagtc aaagaagcca tccaacagca ccaaccagat
     2041 gtcgtgatct gtgtcggaca agcaggtggg cggtcagcag tctcgttcga acgtgtcgca
     2101 atcaatctag cagaagcaag aatccctgat aatgaaggaa atcagccatt cgatacggct
     2161 ttagaagaaa acggtccagc cgcttacttt acctcattgc caatcaaagc aatgacaaaa
     2221 aatgtacaag atcatggtct accagcgtac atctcttata cagcaggaac atttgtctgc
     2281 aatgatatca tgtatcgttt gcttcatttg atcgaaacac aattcccggc aattcgaggt
     2341 gggttcatcc acgtgccatt ctcacccgat caagtgatcg atcgtccagt gggtacgcct
     2401 tctatgtctt tagaagacat tgcttcttct ttgacttatg cagttgaagc agcagtagaa
     2461 aataaagaag atatttccgg aaatgccgga acaactcact aatttagcaa gaaacactgg
     2521 ctggttgcta acgttatgtt agtagccagc tttttgtaat tagaaaggaa agaaacagcc
     2581 gtggaagaat ggcaatccgt ctttgaagaa tggtttccaa aagaaatcag caaaagctat
     2641 cccatcaaaa tttctaaaca atacacaagc agtcaacgtt gggagatcta tgcaaaatta
     2701 acgaaaaaac agcgggaact agtcgataag caccgccgat atctaatcag ctctcgtttt
     2761 atggaagaac attatttagc tgcaacagat tgggtgtttt ctgacttcaa gatcaatccg
     2821 ttttttcgaa caaaacgaag ccagcaaaaa ttgtattgtg agtgtggtag agaattaaaa
     2881 gtgcaataca tcgttaaatc gccaaaaacc ggaaagatcc taaaactagg catcaaccac
     2941 tttgccgatc acctgcatgt ctctccgaca gtggcagctt cgatccatca gggaatgaca
     3001 aaagttgatc tggccttaga cgaattatta tggcttaagc agaagaatat cgattttcct
     3061 gaaggactat ggcaaaaata ctgttttgtc ttgtaccaaa atcgtcggat gaaacagcca
     3121 tatttaccgg atataaaatt agctcagcgg ctcgcagaat ttcgccaagt agaaatgccg
     3181 atttacatag cggattatca agcattggaa aatgaaatca agaaaataag tgaacacatc
     3241 aatgggcagt caaaaaagcg acagataaaa aaagaactat ttgacgactt tgcagaagaa
     3301 ttagtaaagg atgtagaaga attcttgatc aactatcgcg catttttacg taaagattgg
     3361 caatccatcg tatatgaaga agtgccagta catcctaatg cttattttga aacatttatt
     3421 tccgtgttga gaaagacaaa acgtcaacga acaccagaag tcactgctca gatggaatat
     3481 tttgcgaaaa accaacgatt catccaacca aaaatctatc tatttatttg gaagcaatat
     3541 tgtcgctatg gttttacaga agggttcttc gacagcattc ctcggatagt cagaaatggt
     3601 tttttaaaag tattgcgtaa agaacgagaa gctatacaat ctgctgataa gaaagaccgt
     3661 acagtctcaa aagaaaaatg gcaactagta gtgaaagata tccaatctgg aaatgtgcaa
     3721 gagacaatcg acaaatggaa aggaaaacat tatcgtttta ctgaagctca gaaacaagcg
     3781 ttggaatatt atcagaagtt agaagaatca ctgcgtttca atgatgaggc aagaaagtat
     3841 ctgaaggaat tactataaaa aacatactaa gattagaggg gaacatcatt gacgaatgat
     3901 gttctcctct attttctatt tttagattat agtgaagagt gaaagaaagt tcgaacaaag
     3961 ccttgaggac ta
//
LOCUS       DAZKOR010000152         3951 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000152 DAZKOR010000000
VERSION     DAZKOR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.158"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..519
                     /locus_tag="ACG8ZM_002728"
     CDS             <1..519
                     /locus_tag="ACG8ZM_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0493638.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            516..1265
                     /locus_tag="ACG8ZM_002729"
     CDS             516..1265
                     /locus_tag="ACG8ZM_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493639.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1277..1546
                     /locus_tag="ACG8ZM_002730"
     CDS             1277..1546
                     /locus_tag="ACG8ZM_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493640.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            1552..1716
                     /locus_tag="ACG8ZM_002731"
     CDS             1552..1716
                     /locus_tag="ACG8ZM_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493641.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1709..2008
                     /locus_tag="ACG8ZM_002732"
     CDS             1709..2008
                     /locus_tag="ACG8ZM_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493642.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2008..2310
                     /locus_tag="ACG8ZM_002733"
     CDS             2008..2310
                     /locus_tag="ACG8ZM_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0493643.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            2350..2715
                     /locus_tag="ACG8ZM_002734"
     CDS             2350..2715
                     /locus_tag="ACG8ZM_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493644.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            3205..3345
                     /locus_tag="ACG8ZM_002735"
     CDS             3205..3345
                     /locus_tag="ACG8ZM_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493645.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            3342..3524
                     /locus_tag="ACG8ZM_002736"
     CDS             3342..3524
                     /locus_tag="ACG8ZM_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493646.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            3537..3731
                     /locus_tag="ACG8ZM_002737"
     CDS             3537..3731
                     /locus_tag="ACG8ZM_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493647.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(3764..>3951)
                     /locus_tag="ACG8ZM_002738"
     CDS             complement(3764..>3951)
                     /locus_tag="ACG8ZM_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493648.1"
                     /translation="VEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQLKYYSDYWT
                     QTRENELNLEKLIIKKK"
BASE COUNT         1443 a          627 c          847 g         1034 t
ORIGIN      
        1 gtacagcaac gaaacttcat ctatgcgctg ataggcgata tttttatcga tacaggcatg
       61 ccaacggact tctggaagga attcttctac tttcgttttg aaggtgtcac agggcgcaaa
      121 ataagcctga aagacgaatc gaatacaact gtgagtgatg ccaatgtctt agcaaatatc
      181 atcttagatt tcatctttga acatcatatt cctttcaaag aaggctatga gattttacct
      241 gcgaatcaag aatattactt ctacaaatgc atcacaaaaa gagtctgctg catctgtggc
      301 aaaacaggag ctgacatcga tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa
      361 gttgatcatg cagagtacac atttgcagca ctctgcagaa tccatcacac agagaagcac
      421 aaaataggtg tgattaattt caaaaataaa tatcaaatca aagggatcaa attaaaccag
      481 aaaacaatca aaaagttaaa tattggaggg gaaaaatgac agaacatcga agttattatg
      541 cgattatacc agccaacgta aggtacgaca aaagacttaa accaaatact aagttgttat
      601 acggagagat aacggccttg tgtaatgaaa gaggcttttg ttgggcaggc aatgagtact
      661 ttgcagattt atatggtgtg aataaagaga ccatatcgcg atgggtaagt gatttgatta
      721 agtttggata cttgaatcgg gaaatcattt acaaagaggg taccaatcaa ataatcaata
      781 ggtacctacg aattaatcaa taccctattg acgaaaaacg caatacccct attgacgaaa
      841 aagtcaaaga taataataca tctattaata atacatttaa taatacaaaa gaatatataa
      901 gagagttacc gccttcgaaa aaatcgaagg ctaagcccgt ccgtcataaa tacggagagt
      961 ataaaaatgt tcttttgtca gatgagcaga tggagaaact caaaacagaa ttccctaatg
     1021 attaccaaga gcgaatcgaa cgactgtcag agtattgtga atcatctggt aagacttata
     1081 aaaactattt ggcaactatt cgaagttggg caaggaaaga aaaaagtgaa cctaagaacg
     1141 caagcagtgg atacaagcgc acaggaagac gagagaagct tcctgaatgg gcaatcgacc
     1201 aagaagccta tctcaagaaa aaagcgctag aacgagctaa tagacaatca aaagcaccat
     1261 tctaagaggt ggaaaaatga agatcgatta tctagaacta attaatgaaa tagcgaatta
     1321 taaaaagggc gaggaattag acgtcctgag agacgtatat gatcaactcg aagaagctgg
     1381 aattgaagga attaagaatg atcgttcgag ttggagtaaa ctcagatact atttcgcact
     1441 ctatatcgat ggaacgcaat taagaaattt agcatatacc aaattactat tcattgattg
     1501 tgttaaaggc ttgcaaaaac atcttaatga acttgagcag gtgtaaacaa gatggaccta
     1561 aagacattta cagcacagat cgaattaatg catcaagaag ctttaagaca aagtgtgtcg
     1621 tacgaagaca agtggctcaa cacatttcat ggcggacgtg agagcgcact tgatcaagta
     1681 ctcaaattac tgaaaggaga atgtcgggat ggataagaaa gcggcaatgc agcgaattat
     1741 cgaattgact tattcagaag attggcagga agataaaaaa atagttgcag aagtccaaaa
     1801 gctcggtaaa tcaatgtgga ctgaaaagcc caaacggaga acgccgagaa agattgcaat
     1861 ctggcatggt gatcgaattc tagtaacggg taccgctaaa cagttatctg aaattactgg
     1921 tctgagcaaa aatattattt gggatagagc taggagctta tggattgatt caaaaggaag
     1981 acagtttagg tatgtggagg agaaataatg gacgaactaa tcgcgaaagt agagcagtgg
     2041 gcaaaagata agggattgga tcaagcggat ttcagcaagc aaatgttaaa aacggtcgaa
     2101 gaaatcgggg aagttgccgc ttctctagct agaaaagatg aacatggttt aagagacgga
     2161 attggcgatg tagtagtaac cttgatcatc ttggcaatgc aaaatgatat ggatttatac
     2221 gagtgcttgg attttgctta tgacgaaatc aaaggtcgga ctggaaagat ggtcaatggt
     2281 gttttcgtta agtccagtga cctgaaataa ttccattaat ggaagaaata actgaatagg
     2341 aggatagaca tgattgatgc aaaacaagtg caaaaacaga aagatggaat gttgatgttt
     2401 gaagcatatg ttctaccatt tcttaatcaa tttgaagttt tggaatgctc tgccagcgga
     2461 gaagaattgg agtatgttgt aattcgtgaa accaaagaaa acgttcagaa gctaaacgaa
     2521 tttctttgca ctattaactg ttgggatatg atagctccag gatttttatg tccagctatg
     2581 ggagaatttt tggaatattg tcgtcttgaa gatgcaggca ctttggattt agcttatctg
     2641 gtatacaact acctaaacat caacacagat catttgtggt ttggcactgc cgaaagaaaa
     2701 tgggtagtcc gctaaccacc aatatcaatg aaaaaaggcg gtttcctgaa agtggaattg
     2761 ccgccattgc tgtaaggttc tttacgcgac tcaaggtcac agaaatataa atgcaaatct
     2821 cttttgaagt gatccaaagc tctcggaaaa tggaagtttt ttgtttcaaa gaataaatga
     2881 gatttgcgtc aagaacctga gagttatttc caaaaaagct tccaaagaac cgaatgagct
     2941 aaggacatca tgtgtaaaca cataatatag ctctcctttc acggctaatc ataacatgaa
     3001 aaatattcga tgtcagtagg aaaaaaggtg aattatgaaa agaatattaa attatcccgg
     3061 cagtaacaga ttctgcaatt aaactccgaa gctgatgtat cagtatcaac tgtagaatat
     3121 tatgagcgtg gagaaagtca agcagtcaca aatgtaaaaa tcgataacgt caacggtctt
     3181 atctgtttag atgaaggcga tagtatgaat gaaattgctg cagaccatga attatcagaa
     3241 actgctttaa ttgatcaaat cgaattagct agacaatact ttgttccata tgcagatacc
     3301 tggtccaagc ataagaatga catcattttt ccagaggaac aatgagagca actgatccag
     3361 taattatcct tgaggaagcc aaatttattt ggactcacga agagatagag caagcacgct
     3421 tgctcttttc tcaaggagtt aagccgagca aagtagctga aataatgggt caaaagattc
     3481 ttgatgtcgg attgcttttg ctccatctag cagaaaaaat ttgatttgag gtgaaaatga
     3541 tgattcaact tgcaggcatc caaacaggga aaatttattt ttctggagaa agcaaaagcg
     3601 aagccaacca atggctattg aaaacttaca cgaacaataa gaagctaagg aaaaaatatc
     3661 ctgatttgtt gctaaaggat gatcagatta tgccggaacc gatgattttg acccgtaagg
     3721 agggaagttg aaactaaaaa accagccgtg tgggctggag ttgctatttc tttttaataa
     3781 tgagtttttc taaatttaat tcattctcac gagtttgcgt ccaataatca gaataatatt
     3841 ttaattgttt aagagtagga atgtcgtctt cggtaaaatt agaaaataat tctggagaat
     3901 tatctttttt taattgttct aatcctgatt ctttaaaacg ttgttcaacg a
//
LOCUS       DAZKOR010000153         3923 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000153 DAZKOR010000000
VERSION     DAZKOR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.159"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACG8ZM_002739"
     CDS             complement(573..1130)
                     /locus_tag="ACG8ZM_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493649.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACG8ZM_002740"
     CDS             complement(1309..1920)
                     /locus_tag="ACG8ZM_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493650.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACG8ZM_002741"
     CDS             complement(2033..2290)
                     /locus_tag="ACG8ZM_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493651.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACG8ZM_002742"
     CDS             complement(2887..3165)
                     /locus_tag="ACG8ZM_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493652.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACG8ZM_002743"
     CDS             complement(3199..3450)
                     /locus_tag="ACG8ZM_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493653.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DAZKOR010000154         3896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000154 DAZKOR010000000
VERSION     DAZKOR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.160"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..1104
                     /locus_tag="ACG8ZM_002744"
     CDS             289..1104
                     /locus_tag="ACG8ZM_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493654.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1230..1487
                     /locus_tag="ACG8ZM_002745"
     CDS             1230..1487
                     /locus_tag="ACG8ZM_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493655.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1739..2311)
                     /locus_tag="ACG8ZM_002746"
     CDS             complement(1739..2311)
                     /locus_tag="ACG8ZM_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0493656.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2327..2932)
                     /gene="fic"
                     /locus_tag="ACG8ZM_002747"
     CDS             complement(2327..2932)
                     /gene="fic"
                     /locus_tag="ACG8ZM_002747"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0493657.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            complement(3333..>3896)
                     /locus_tag="ACG8ZM_002748"
     CDS             complement(3333..>3896)
                     /locus_tag="ACG8ZM_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0493658.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
BASE COUNT         1294 a          715 c          582 g         1305 t
ORIGIN      
        1 aaaaataaga aattcaaaaa agaaaaaact ttccttaaaa acaaaaaata ggagtattca
       61 gtttcttaat agttttgtaa tagttgtatg tagaagtttt cttaatagtt tttgtaataa
      121 tttattaatc gtagtttata taacttattt gtaagtgact agagaattaa ataaaaaata
      181 attactatct agtacgaaat taattacata ggataatata ttaaggtgct ggactacaag
      241 taaaaaatac tgtatgatca tgacagattc aagaaaggag caataagatt ggttaagata
      301 gcaggacttt ctaaaggtgg aaaaaccgtt tcacaacttt ataaacaaaa ctacagagac
      361 tggagacagt tcagaaaaaa cattaaggat tcttacttta ttctaccaac agaacttgaa
      421 aaatatttac cgaataacaa agctattaat ttatatctgt attactgttt taaggcaaag
      481 aatgaaggcg gggattcctg gcattcggtc aataccatag cagagtctct aggagtgaca
      541 actaaaacta taaacacctg gaataaaacc ttagttgatt taggtatgat tgcacgtata
      601 gacgataacc atctcagtaa atcgacaatt ttacttcctc tctctagtta ctataaatta
      661 gcccttgata gttctttaaa ggatgttacc gagagcactg tccatgaaat tgatggcaac
      721 ttggttgcag tttatcattt gtttgaatgg agaaaaaatg aggctgatga aaattataac
      781 gttccttata acacactctg ttgtgttttt aaaagaaaaa cacaaaaaga tactatctat
      841 aaatttatct tggtccaaga tgagaaaata agtaatttcg aattaagcac tccttctagt
      901 aagatttatg acgatgcata tagatttgaa tgtcaagatc tagaagccct cctttccgca
      961 aataatctaa acaatataga acatgaaaac tttgttgtaa acactagatt taatttagtt
     1021 tctgaaaagg actcagattt gttagatttg ctgatagagc ttactaaaaa tctagataag
     1081 aaagaaaaca ttactgtact ataactagca tcggtccatc attaagtttg gactattaga
     1141 acacttgcta ttacagaaaa aggtatgcta gccacgctta ctatttattg gtatcaaaca
     1201 agtatcttaa tttgaaagga agatgattta tggctaattt atatatagag aacaaaggag
     1261 acttgaatgc cattattact ttaatcgtag ttatggtaaa atcaataggt aatatcacaa
     1321 taggaatgct gtttattata ttagcagcag tgctgatttt tatgatagtt atttggttct
     1381 taaatgaaag atctaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
     1441 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
     1501 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
     1561 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
     1621 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
     1681 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
     1741 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
     1801 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1861 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1921 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1981 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     2041 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     2101 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     2161 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     2221 tcttctctac gccgttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     2281 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     2341 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     2401 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     2461 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     2521 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     2581 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     2641 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     2701 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     2761 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     2821 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2881 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2941 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     3001 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     3061 ttgcacagtc tagttgacct ttgatacacc tctatattta tagagaatct aaaacatcaa
     3121 catggcatac tcaaaattct aaatattagg gataaagaat tctcctatac aatcaaaaat
     3181 ttcctttatt tcgaactccg gtcacctaac tctattgatc tcttacaagc caaagaatat
     3241 ctctatacta caaagaaaca atagggtgcc tggttggtct ctttgtgttt tattttagaa
     3301 tctagaacgt aaatattctt gtcattctga ttttatctaa gaatatcaat taaatctttt
     3361 tttgcagcct ctgaagcggg cattacagaa aagaccagtc caataaccga agatacccca
     3421 accgctagta aaatagtaaa tagatcaagt gacacgttta cctttatcaa agaaccaaca
     3481 ctataagcaa gagccattcc taataaatag ccaattaacc ctcctgaaag agttaaaatc
     3541 aaaccttcta aaaggaactg cagcataatc gattttcgag tagctcctag ggctcttctt
     3601 ataccgatct cttttgttcg ttcagataca gatatataca tcatgttcat tacacctacc
     3661 cctgcgataa ataaagatat acctgctaca gctgttataa aataagtgat cgtactgagg
     3721 atttgaccaa ttcctttcgt taacatagcc gtatctaaca cttcatattc ccccaaatgt
     3781 ttcaaactac ctttttcttt caattggttt atcactttag aggtcacctt gtcaggttca
     3841 actccttcct ctaaagttaa cgttagagac gatgtatcct tttcagactt aaaata
//
LOCUS       DAZKOR010000155         3865 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000155 DAZKOR010000000
VERSION     DAZKOR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.161"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..1511
                     /gene="gndA"
                     /locus_tag="ACG8ZM_002749"
     CDS             90..1511
                     /gene="gndA"
                     /locus_tag="ACG8ZM_002749"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0493659.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            1704..2390
                     /locus_tag="ACG8ZM_002750"
     CDS             1704..2390
                     /locus_tag="ACG8ZM_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0493660.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            2387..>3865
                     /locus_tag="ACG8ZM_002751"
     CDS             2387..>3865
                     /locus_tag="ACG8ZM_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0493661.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFI"
BASE COUNT         1306 a          667 c          827 g         1065 t
ORIGIN      
        1 aaatatgttg ttctttagtt tgcaatgtag ccgtgttaag ctataatgta agagattagg
       61 gagaaatccc gtatgtagga ggagttcata tgtcaaaaca acaaatcggc gttgtcggta
      121 tggccgttat gggtaaaaat ctagccttga acatagaaag ccgcggctat tcagtggctt
      181 tattcaatcg tacaggggct aaaacaaccg ctgtagtaga agaacaccca gataaaaatt
      241 tcaaagcaac atatacaatc gaagaatttg tagaatctat cgagaaacca cgtcggatct
      301 tgctaatggt caaagcagga ccggcaacag atgcaacaat ccaagagtta cttcctcacc
      361 ttgataaagg cgatattttg attgacggag gaaatacatt ctttaaagat acgatgcgtc
      421 gtaatgaaga gttagccaac tctggcatca actttatcgg tacaggcgtt tccggtggtg
      481 aagaaggagc tttaaaagga ccttcaatca tgcctggtgg acaaaaagaa gcctatgcgc
      541 tagttgcacc aatcttagag caaatttctg caaaagcaga agacggagca ccatgtgtca
      601 catatattgg cccaaatgga gccggacatt atgtgaaaat ggtacacaac ggtattgagt
      661 atggagatat gcaactgatt gctgaatctt atgacttaat gaagaatatt ttaaaccttt
      721 cagtagaaga aatggctgat atctttaaag aatggaatca aggcgaatta gacagctatt
      781 tgatagaaat cactgctgac attctgacac gtaaagacga tgaaggaaca ggtaaaccag
      841 tagtagatgt gatccttgat gcagctggga ataaaggaac aggtaaatgg acaagccaaa
      901 gcgcgcttga cttaggtgtg ccacttccat tgatcactga atctgtattt gcccgctata
      961 tttctgctta taaagaagaa cgcgtacaag caagcaagat tttgtctcgt acgaacgatt
     1021 ttgagttcac gggagacaaa aaagaattgg tcgaaaaaat ccgagaagcg ttgtatttca
     1081 gtaaaatcat gagttatgcc caaggatttg ctcaattacg tgtcgcttca aaagaatttg
     1141 attgggactt gccatttggt gaaatcgcaa aaatctggcg cgctggatgt atcatccgag
     1201 ctcgattctt gcaaaaaatt acggatgcct atgacaaaaa tcctgagatc gaaaatttat
     1261 tgctagatga ttacttcgtt gaaatcacga aaaaatatca gcaatcagtc agagacgtcg
     1321 ttgccttggc tgtccaagct ggcgtacctg taccgacttt ctcatcagcc atcgcttact
     1381 tcgattcata cagagcagaa cgtctgcctg caaatataat ccaagcccaa cgcgattact
     1441 ttggcgcaca tacctatgag cgtgtcgaca aagaaggaat cttccattac tcatggtacc
     1501 atgaagaata atagtctttc ggctgttagg aaatgagaaa ataaaaagag gacgaaagag
     1561 ctgaagtcga ttgttatgag tagacttcag ctctttttta aaattgctat ccatgataaa
     1621 atgactatag atttttaagc atattatcgg tataatgata gagtttttaa aaagccagta
     1681 gaatagaaag gataaaataa taaatgagca atattctaat cattgaagat gaaaagaact
     1741 tggcacgatt tgtcgagcta gagttgaagc atgaagggta taacgcagaa gtccactaca
     1801 acggccgaac tggtttagat gcggcattaa ataatgagtg ggatgccata ttgttggatc
     1861 tgatgctccc agaattgaac ggtttagaag tctgcagacg tgtacgccaa gtcaaaaata
     1921 caccaatcat tatgatgact gcaagagatt ctgtgatcga tcgtgtatct ggacttgacc
     1981 acggagcaga tgactacatc gtcaaaccat ttgctatcga agaattatta gcacgtcttc
     2041 gtgcattgtt gcgtcgaatc gatattgaag gcgataaaaa tgtggctaaa caaactacta
     2101 ttacttatcg cgatttgact atcgaaaaag aaaaccgtgt cgtacgtcgc ggaaatgaag
     2161 taattgaact aacaaaaaga gaatacgagt tgctgcttac tttaatggaa aacgtgaacg
     2221 tggtattagc acgtgacgtt ttactaaata aagtgtgggg atacgaaaca gaagttgaaa
     2281 caaacgtagt ggacgtatat attcgttatc ttcgcaacaa aattgacgtt cccggggagg
     2341 aaagctatat ccaaactgta cgcggaacag gttatgtgat gcgctcgtga taaagccgaa
     2401 aaaatcggaa aaaagagaac tgaaagggcc ctccttgaca atcaagtggg cctttgcgag
     2461 ttctttcttc atattcgttg tgtttacaat tttcgctgtc attacctata aatcatctat
     2521 caatctgatt gtggcaaaag aaagaaacaa tgtagagcgt acgatttcag aagtagcttc
     2581 tcgtttggct aactcggatg aagaactgac attagtaaat acgtatcgta atctgacgaa
     2641 agcgacaaat gacggagatg aggtcgtaga tcatacttca ctggtcgaag gaaacttaat
     2701 gaagatcgat tcctttatag ctgagctagg gcagccatca atggatcttt atgtttatga
     2761 tttggacgag aacttgattt ttaaaaccca tgaaaacagt cgaaacctga ttcagactac
     2821 acgtaaagtt ccaacgatcg ttacactaga aggaaaaact ggctttttat ctatccagcc
     2881 tatatattct aaaggtacaa gagaaaagat cggctatgcg caaagcttct atgaattatc
     2941 ttctttctat gatattcgaa ataagctatt attgactctt attgttttag aagttgtttc
     3001 tttgatctta agtagtattt taggtttctt tttatcttct tatttcttaa aaccactcaa
     3061 ggtactcaga gatacaatgg atactatacg taaagatccg caatcagata tccacatgcc
     3121 tgaaatcgac acaaatgatg aattggcaga tttagctgaa atattcaatg agatgcttga
     3181 ccgtatgcgt ttgtatattg aacaacaaga gcaattcgtg gaagacgtat ctcatgagtt
     3241 aaggacacct gttgcaatta ttgaagggca tcttagtctg ctaaatcgtt gggggaaaga
     3301 tgatcctgag atattagaag aatcattaag cgccagcatg caagaaatca gccgtatgaa
     3361 aagcttagtt caagagatgc tagacttatc aagagcagaa caagtagatg ttcattatgc
     3421 gaatgaaaca actaaaggaa aagaagtcac ctatcaagtt tataataatt tcaagatatt
     3481 atatccagac tatgtgatca ctttagatga tgatttgaca aaagaagtga ccctgcaaat
     3541 ttatcgtaat cactttgaac aattaatcat tatcatattg gataacgcag taaagtattc
     3601 aactacacga aaagaagtcc atatctctat ttcttccact ctgaatgaat ttgagatcgc
     3661 gattcaggac tttggcgaag gaatacccaa agaagacctt aataagatct tcaatcgttt
     3721 ttatcgagtg gataaagcaa gagctagaac gaagggtgga aatggtctgg ggttgtctat
     3781 cgctaaacaa ctggttgaaa actataaagg acgtatctta gcagaaagtg tagtagggca
     3841 agggacaatt tttagaatct ttatt
//
LOCUS       DAZKOR010000156         3424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000156 DAZKOR010000000
VERSION     DAZKOR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.162"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACG8ZM_002752"
     CDS             complement(<1..2177)
                     /locus_tag="ACG8ZM_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0493662.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACG8ZM_002753"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACG8ZM_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          966 a          754 c          539 g         1165 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atat
//
LOCUS       DAZKOR010000157         3407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000157 DAZKOR010000000
VERSION     DAZKOR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.163"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..878
                     /locus_tag="ACG8ZM_002754"
     CDS             78..878
                     /locus_tag="ACG8ZM_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFQ0493663.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            894..1772
                     /locus_tag="ACG8ZM_002755"
     CDS             894..1772
                     /locus_tag="ACG8ZM_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFQ0493664.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            1785..2765
                     /locus_tag="ACG8ZM_002756"
     CDS             1785..2765
                     /locus_tag="ACG8ZM_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0493665.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            3036..3128
                     /locus_tag="ACG8ZM_002757"
     CDS             3036..3128
                     /locus_tag="ACG8ZM_002757"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0493666.1"
                     /translation="MPRRSFDEIFKITAVKLVTEEGFSVKEVSL"
BASE COUNT         1173 a          559 c          712 g          963 t
ORIGIN      
        1 taactcttga cagataatca aaaagaattt atagttgtta ccgtagtagc tactaaaaat
       61 aaagtgagga ttaatcaatg gaaacgatta ttcaaatcat gaaaaaatat gatttttcag
      121 agatggagac aagagtgtat accacactat tagaaaaagg taatcttaca ggatatgaag
      181 taagtaaaat ctcaggtgtg ccacgctcga aagtatataa tattttggaa aaactattaa
      241 aaaagaattt gattgtcgtt aataaatcag aacctaaact ttatcatgcc atctcggcaa
      301 atgagttttt agagaaatta gaaaaaagtg ttaaaaatga tctttctttt cttaccaaga
      361 atctagggat gatcaaagaa aaagatgaag aagaaatgct gtggaaagtt gatggtatcg
      421 agtatgttct ggataaagta gagcatttgg tgaaaaacgc aaaagaaagt ttgctcattc
      481 aagtttggca tgagaatttc acggattcac tactaaaggc attgcagcaa gctgaaaaaa
      541 gagttgataa gtttgtactg attttattca gttctaccca tgaatatgat cttcccttag
      601 aaaagtatta tattcatggt tttgaaacag acaaacttgc tgactttgga gcaagatgga
      661 tcaacatcgt cgccgatgag caagaagtcg tgtttggtac cattaacgaa gaactgcaat
      721 caaccgatgt cacttggaca aaaaaccacg ccatggtcaa tctagcaaaa gaatatgtca
      781 aacatgatgc atatacacta aaagtgattg cagaatctcc tgaagaactt aaagcaaaat
      841 atggtgaggg ctttgaggga atcagaaaga tttactagta ggaggaattt tttatgattc
      901 ggaatatttt gctgacacta attgtcgcaa tcaatctata ttttattatt tactttctaa
      961 gagacctttt caaaaacaaa caaaatttca aagaagaacc aggggatatg cgtctgttgc
     1021 ccttcacgtc atttatcatc ttctttcttt caacatttgg cgtttccgac tttgctatcg
     1081 gaaccgtcct ttatccaaag ttgaaatggg tcagcatgaa aaaactaccc ggaacgttga
     1141 atacccaatg tgtagtaccg gtagccgtca tggcattgtc ttacatcact gccatcaacg
     1201 tagggatcaa gacactagct gtttgtatta tctgccaaat tatcggtgca tatttaggac
     1261 caagatttgt cgtgaagtta ccagagaaaa cgatcaaact gtttgtcggc ataggattgg
     1321 tcattgctgc attgttgatt tttgcaggac aaatgaactg gattccatca aacggaacag
     1381 ccagtgaatt gtatggcgga aaattaatct tggcaggatt tttactcttt gtctatggtg
     1441 cattgaacaa tatcggcatt ggttcttatg cgttaacgat ggtaactgtc tacttgctag
     1501 gcttgaatcc agttgcagcc tttcctatta tgatgggggc ttgtaccttc tcagtaccga
     1561 tcggcagtgt tcaattcatt aaatttaatg aatacagcag aaaaatcaca ctttttactt
     1621 caaccttcgg tgtattgggg gtattggtcg ctgtattcct agtcaaatct ttagatacct
     1681 atatcctgaa atggatcgtg attttggtct tactatacag tgcatatacg atgttgggca
     1741 gtcaattaaa aaaagttaca gcagctaatt aaggagcgat tcaaatgtta tttcttaaaa
     1801 aagaagatat tatcaaaagt ttttcaatga gagaagccat cgatgcggac aaaaaagcac
     1861 tgtcattgta ttcagcagga aaggcaagtg tgcctcttcg caccaatatc gatgtgccaa
     1921 aaagcaacgg acaaagctta tatatgcctg cttatgtaga aggtggcgaa ggcgcgctag
     1981 gtgtcaaaat cgtttccgtg tatcctgaaa acatcaagaa aaacttacca agtgtaccag
     2041 caacgatgat cgtattggat ccagaaacgg gtatggtatc cgcttgtttg gatggtactt
     2101 atttgacaca attaagaaca ggcgctgttc aaggagcagc aacagaactt cttgcaagag
     2161 aagatgctaa aattggcgcg ctgattggaa ccggcggaca agcacagtca caattagaag
     2221 caatgttgac cgttcgtaaa ttagaagaag ttcggatttt tgatattgat tttgagcgtg
     2281 ccagccaatt tgctgaaaaa atgatgcaac aattttctgt aacgatgcgc ccaacaaaaa
     2341 ccagtcaaga atgcgttgaa ggagcagaca tcattacaag tgtgaccact tcaaaacgtg
     2401 cgacattctc tgctgagtgg gtcaaaaaag gtgcgcacat caatggagtc ggtgcttaca
     2461 caccggaaat gtgtgaaatt ccacgagaaa taatcaaagc cgcagatgtt gttatttttg
     2521 ataccatgga cggtgttcta aaagaagctg gggacttcat ttcaccgctg caagatgggt
     2581 atattcaaag agacagctat catggggaac taggccaact gatcaatgaa gaattgatcg
     2641 gtcggacaag tggagaacaa atcacgatct tcaaaacagt tggttcggct gtattagatg
     2701 tggtagttgc gacagaaatc gttaagaaag ctaaagaaaa taatttggga aaattgattt
     2761 tctaattaaa aaggctgaaa ttaaaacctt tattattgcg tttaaaaaaa gaaaacaaag
     2821 aagtttgtaa tcaaatggaa actgacccca taatttaaat aaaataaaaa acatctcttg
     2881 ttgaattcta gtaatgaagc gaccctaaaa cttagaccaa aaaatctaac ttttgggtgg
     2941 agttgtcaag aaaaaccggg cacttagtta aaaaataagt tggacgggtt atactaaaat
     3001 tgacaccaaa ttaagagaaa gcgaagatct ggattatgcc aagacgttca tttgatgaaa
     3061 tatttaaaat taccgctgta aaactggtaa cggaagaagg gttttctgtt aaagaagtta
     3121 gtctatagct agaagctcgc tctaatagcg tgtattgatg agttcaagaa gttgaaaaat
     3181 atggataacg tacttttctc ggtagaggaa gtcatgaaat ccattcgcag aaatgaaatt
     3241 acacgcttag aaaaagagaa taaacggtta aaagaatagg tagaactcct aaaaattacc
     3301 gaagtcttcc tgaaagaaaa ggctcttcgt caaattgtgt tggcgaagac aaatttgaat
     3361 aaaacttagt ccgatatgcg gcgcgttctg tgcgccgcat atttttt
//
LOCUS       DAZKOR010000158         3392 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000158 DAZKOR010000000
VERSION     DAZKOR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.164"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            508..813
                     /locus_tag="ACG8ZM_002758"
     CDS             508..813
                     /locus_tag="ACG8ZM_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0493667.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDFYKVWDSEVFFLAIEDSLLIN
                     LYYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1327..1400)
                     /locus_tag="ACG8ZM_002759"
     tRNA            complement(1327..1400)
                     /locus_tag="ACG8ZM_002759"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1364..1366),aa:Ile,seq:cat)
     gene            complement(1590..2600)
                     /locus_tag="ACG8ZM_002760"
     CDS             complement(1590..2600)
                     /locus_tag="ACG8ZM_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0493668.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYTINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSAMKYGIPLTLETPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            complement(2597..3016)
                     /locus_tag="ACG8ZM_002761"
     CDS             complement(2597..3016)
                     /locus_tag="ACG8ZM_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFQ0493669.1"
                     /translation="MEKYLNTFSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVEVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(3086..3226)
                     /locus_tag="ACG8ZM_002762"
     CDS             complement(3086..3226)
                     /locus_tag="ACG8ZM_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0493670.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
BASE COUNT         1013 a          638 c          542 g         1199 t
ORIGIN      
        1 catcagtaca tctttggcta cttcggtagt ttcgaaaaag atgtacgtga tgacattttt
       61 tatatttcgg gtatgaaatt cgggtatgta ttttagtttt ttaattttta ataacaataa
      121 aaaagcactc aaattgagtg ctttttttaa gttcactatc actaaaacta tcactagttt
      181 cattttaaag caaacatgtt tctatcactg attttttaaa tctagcgaca aatccttatt
      241 ttacaggcgt tcgttcaatt ctttagcaag gtcttcaaat cctggtttgc ctagaagtgc
      301 gaacatgttt ttcttatgag aaccaccctt ttcatttgtt ctaaagatat ataaatgttg
      361 atacatcaat gtttattctt atttttctat ttacataaat ttcgtcgttt ttgataaatg
      421 ggtactagtt tgggtaccgg ttgctaccct atttttatcc tttacaaaac aaacattagt
      481 tcgtatactt tcgttgaggt gaaaaacatg acaatcgagc tagaggaaca tctgctttgg
      541 tttaaatacg atccatcaga aaaatttgtc aaagattttt ataaggtgtg ggattcagaa
      601 gttttctttt tagcaatcga agatagctta ttaatcaatc tctactattc taataagaac
      661 tatttcaaaa ttcctgctgc gaaaactaga atgaagaagg gtgtatactt tttgtttgat
      721 atcgtgactg acgtgccgga cgcacgtagc gatcatcggc gttatgacta tataaagtat
      781 actttcgttg atccagaaag gtataaagat taaagtaggc tacctaaaaa ggtagcccgg
      841 aacgaatttt atcaccatac ttgtgaaagg agatattttt taagttagta ttaagattgt
      901 gtaatatgat gatatctata ttttatagta tcagtgctat aaaatcaaaa ataagtcact
      961 aattaactac cactccaatt gtaagccttt tttctcactt tttttcaaaa atatggtatg
     1021 ctttttaatg gcttcaaata taaaagagtt taaagcgtaa cacacttatg gggaagtggt
     1081 ttggggtgcg ctttaaactc ttctttatta ttatctcaca atttaaccca aatgtctttc
     1141 tatttaaaaa tcaaagtaaa acttttcaaa tatacagaag tataactatg tgaaacatcc
     1201 tttcattaat ccataaaagg atacataaaa aagccactca tttgagtggc aatgaagaaa
     1261 agctttagct tgtataatac tcttcaaaaa aattctaaca cagaacgatt caaatggcta
     1321 cgttaatgta ccctgtagga ctcgaaccta cgaccggacg gttatgagcc gtctgctctg
     1381 accaactgag ctaagggtac tggttgttgc cacataaagc cataaacaat caaccagtag
     1441 aatgtgtggc aacaaacctg ttatcgcata gcttggagtg tgactattta tgggtgatag
     1501 tgaagatatg cgataacatc actattttat cgaataattt ttatagttgt caatatagtt
     1561 atgtactgct cctcaacgag gagctatttt tatcgtttag gaatatttaa ataccaacgt
     1621 ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt cgttgcccgc
     1681 atcattacat agactttctt actaggaaaa ttacgcatat tgaaagatac atgataacca
     1741 acatttccag aagtattata agcttggttt acatctggtc tataaattcc atcagctctt
     1801 actcgagcta attctttccc agtattgtag tccataatga aaatatactc gtatttatag
     1861 ttagcgatgt gccatccagc gacgtgtaag ttcgcatttt cgatttctcc aaactgatca
     1921 atgtgggcgt aatttgttcc atctgtcagt gtaggatttg ctgcacctgc tctagttgga
     1981 tcaatgactg gtttatcatc tgaagttgtc ggattttcat cggtaaatcc atgagctaaa
     2041 tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta aggatctgta
     2101 tgatcgcccc agatattttg tgttacccat aaatgcgatt tgattcctgg ttggttataa
     2161 ggcgtttcta atgttaatgg aataccatat ttcattgctg aatctctagc caattcaacg
     2221 tatgccttgt agttcttttc aaacgttact ttatcgtgcg tatgttgcag ttcgatttgc
     2281 acaggactat ttgcattagc atatgaacca gcgccatatt gtacataacc aggttgtcca
     2341 acttggtaaa cgatcccacc atcgccaaca atatacgctg tataggcatt gtaccaagaa
     2401 cgtttcatgt actgcgcttc atttcgtcct gtcgcagttt cgtttgctgt ttcatgcaat
     2461 aggatgtatt ggttatttgc tacttgagaa ctaccttcgt ttgcgcccaa attgaattca
     2521 ttgttgatcg tgtaagcaaa ggcgtttatt ggcaataaaa aaagagctat aatagctcct
     2581 acaaagattt tctttttcat ttggttcctc ctcggtcttt attttcagta tcatttcgta
     2641 tttgttggaa tacctctttt aacttatctg gcaaaggaag gaataccgaa atgttttcta
     2701 aaaaactaat cccttcattt gcaacataga acataataac tatttctctt agaggtatag
     2761 aattcccaac gattttctgt acttcaaccg caacggcaat cacaacgaag attaagactt
     2821 tcttcaacaa tccttcgaaa cctatctcac tacttatttc tttcaatttc catgccttag
     2881 caagtccagt taagaagtcc acacccacaa gaaaaattaa aacatgtagt atattgtcca
     2941 tccctcccaa taaaccaaca attgtcccgc ctatcacccc tgtcattact gagaatgtat
     3001 ttaaatattt ttccaatatc tatttctcct ttatgcaaaa aagcgcactc gtttgagtac
     3061 gctttttatt taactttgtg cttaattaaa tttcatttcc agtaatctgc ttatattggt
     3121 cttttgttaa acagttcatg tttacatagt caagtaaatc ttggtccgta taacatcccc
     3181 aatcataaaa ttgttttata ttttcaaagc ccggaaaact attcattact tttccctcca
     3241 ttcttaatac ttgcgacttc catcgtcaat tgagctaaca tttgttgcat tttttcttgt
     3301 tcctgtaccg atatcgccaa ttgttttgtc agtagagcat ttaattgctt caactgatcg
     3361 acatcttcaa tatcgtcaac cgctgtgcca ct
//
LOCUS       DAZKOR010000159         3303 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000159 DAZKOR010000000
VERSION     DAZKOR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.165"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..190
                     /locus_tag="ACG8ZM_002763"
     CDS             <1..190
                     /locus_tag="ACG8ZM_002763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493671.1"
                     /translation="DLEKLTKQRDMKNFNNCVVLIIITELTLFTNIYYIHEAQSSLIE
                     DWAIFMAKEGKEMFQNKG"
     gene            203..478
                     /locus_tag="ACG8ZM_002764"
                     /pseudo
     CDS             203..478
                     /locus_tag="ACG8ZM_002764"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            505..804
                     /locus_tag="ACG8ZM_002765"
     CDS             505..804
                     /locus_tag="ACG8ZM_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFQ0493672.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            978..1529
                     /locus_tag="ACG8ZM_002766"
     CDS             978..1529
                     /locus_tag="ACG8ZM_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFQ0493673.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1675..1968
                     /locus_tag="ACG8ZM_002767"
     CDS             1675..1968
                     /locus_tag="ACG8ZM_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFQ0493674.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1961..2257
                     /locus_tag="ACG8ZM_002768"
     CDS             1961..2257
                     /locus_tag="ACG8ZM_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493675.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2332..>3303
                     /locus_tag="ACG8ZM_002769"
     CDS             2332..>3303
                     /locus_tag="ACG8ZM_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0493676.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGM"
BASE COUNT         1236 a          455 c          661 g          951 t
ORIGIN      
        1 agatttagaa aaacttacta aacaaagaga tatgaaaaac tttaataatt gtgtagtttt
       61 gataattata actgaattaa ctttatttac aaatatatat tatatccatg aagcccaatc
      121 ttctttaata gaagattggg ctatttttat ggcaaaggag ggaaaagaaa tgtttcaaaa
      181 taaaggataa cgttatgtca caaagggagt aatggacagt ttgccggtgg aattacaagc
      241 tttatgttgg aatctaatcg atcaaaacgt tcaaaagcaa tttcctttag actatcttca
      301 aatttttgaa ttttctacgg aaaaaggaaa tcaaaagttg gttcatcgtc aagaagaacc
      361 ggaagaaaga aaagagtatc tgatttcacc taagcttcgt ttgaaatcag tgtctcaaaa
      421 aatatgggcc attgattcgg gtgagtatca aacgatgtta ttaccagaag aatactagta
      481 tctaaaagaa aggaacgaaa ggacttgtta gaaacggcta tcgaagtact ggaaaagtgt
      541 gcacagctgg tcactgcttc tgaagagtgg ggctatgaat ccgttacgat ggaaaaagag
      601 gaaattgaaa tgggtatgct tcctgaagat gttcatctac cacgtttagt gatgacacat
      661 ctttatattt attgcgcacc agaagatgga aaagactatg tagtttattt cattacagat
      721 attaccagcc agcgagaatt tgttcgtggg ttactggtag aaggacgatt ggtttggagt
      781 cagattggag gaacggatga ataagatcga ttatttgaga atgattgcga aatgtttaga
      841 agatatcctt accgcgattc atcaaattct gaagaaaaaa ggagaaagaa tatgaaatat
      901 tttcaactag tattgacact tataacggca atggtaattg aagtgcttga acaaaaaata
      961 gcataggaga aaaagagatg attgaaaaag aattagtgac cacacaaaca gaagtttggc
     1021 gagatcctca tcatacccat cgctatgttt tcaaacgaca atgggcgatc aaaggaaaag
     1081 aagcggaaga taaattagca gtagtcatta ctattcgtcc aacagataca gaaccattta
     1141 cgaatgattt gagtatgtta ttaatcgaaa agaatatccg acaattagga tttactggtt
     1201 ttattgctgt gaatcttttt tcgtacacaa aagcgaaaag tcctcgggta tttcttcgag
     1261 gaaatgatga acaatctttg gaaatattaa cgactgtttt tacagaaaag aaaatcaata
     1321 ccattatttt tgcttgtggt tcgattacag cgacgagtca agtggcttat gaacaagcaa
     1381 agactattta cgatcaattg agtgcgaaac aaaagaagat gaccaaagta ttgataaatg
     1441 cagaaggtaa gttatcccac ccactaagta ttcatgttcg taatgaatgg caattggcag
     1501 atagtcaatt attatttcaa gaaaaataac attttatttt ctcatgttac ttataagtag
     1561 ttttacatgc tttttgactt taaaaataca tgtgatgata catgttatat gaattatcaa
     1621 tgtacccagt aatatgctat tttcattatg taaaaaataa cagggggtgt gtagatggag
     1681 attgatgaaa cttgtttgag taactcttat aaaagtatga atgacttttt tgaggaaatg
     1741 gaactggatt cagaattgct ctatcaattt tatcttgctt ttcgagggca acagatttct
     1801 tttccaatga aaatgtacga tcgtgagtta gttagaaaaa gaatcgaaaa catgatagaa
     1861 caggaaaaaa ctgtagatat ccgtcaattg acagaagttt atggtttctc tgcaagatgg
     1921 attcaaaaag taattaaaca aaaagaaagg agacgagtac atggctaagc tgattgtatt
     1981 aggtggttta atttacggtg gatattggtt atatcaatat atgcttgcgc atccgaatat
     2041 agataaagga aaagtaatta agacagttgg ggtaatcata gttggactca tagctctaac
     2101 taagaaaaat aatactaata gcccaaaaat aatgagaact tatcgctgta gaggatgtgg
     2161 aagggaaatt tctactagtt ttaatccatc tgagagtcct caaggaagag accgctgcca
     2221 tacgggctat cgacattatt gggaaagaat cgattaaaag attaccattt agatcaatta
     2281 attgtctgat agaataagta cgtgagggag aaattgaaag ggggaaatat aatgaaaaaa
     2341 tggctgttat tactaatagg aatgggaatt ttattcactg gttgtacatc tgagaatacg
     2401 aggactaaag cgactataga aactgataca acaattgaga ctaaaaaaag taaaacgcaa
     2461 tcaactgaac aaactacaac agaaatgaag caatctgtgg aaacaagtgc aagttcaatc
     2521 actgaatctt cttctgaaga ggttaagaat actttgtggg atacgaataa agcttccaaa
     2581 ttagaaactt ttgtcacaca atggggaaag acattaggtc aagaatataa aagctacaat
     2641 cttcagaata atgtaagtct gtatggcact cctttaccac aagccgtgat taatggcgat
     2701 tggaaaatgg caatcaatga agcaccagtt accgtacaat ggtcagaaga tggaactggt
     2761 catgcagatt tcaatttagt agcagtttat tctgatgtag aaactggaga atatttaggg
     2821 cagcatgttt actttttcgg ttttcaaaat ggacaaccta aagtttattt aacgcaacaa
     2881 aatcaaggta atgaaaataa ttatctgtac tttaacgaaa ctcaaaatca acagttaaaa
     2941 caaggtttca gtgatattgt gaatggacaa actcctcaaa caccagtagt agaagaatct
     3001 actcaacaag caacaactac aacgacacaa tctacaagta gttcaggaat ttcaagttgg
     3061 gaagctgcta aagaatctgt gctaaataat aaagctttat ggttgtatga ttctcctgat
     3121 aaaaatgtat caaatactaa tttaattagc acaaatgaat atatgaaaga tctaatgcaa
     3181 gatgaaaatg gaagctacta ttcaattaat attgtaacgg atgatgatgg aaacacgacc
     3241 atggctggtg gaaaacaatt tcgtgtttat acagatggaa gaatttctca acgtattgga
     3301 atg
//
LOCUS       DAZKOR010000160         3268 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000160 DAZKOR010000000
VERSION     DAZKOR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.166"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACG8ZM_002770"
     CDS             complement(451..1761)
                     /locus_tag="ACG8ZM_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0493677.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACG8ZM_002771"
     CDS             complement(1773..3191)
                     /locus_tag="ACG8ZM_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0493678.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT         1002 a          668 c          507 g         1091 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgt
//
LOCUS       DAZKOR010000161         3221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000161 DAZKOR010000000
VERSION     DAZKOR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.167"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG8ZM_002772"
     CDS             301..585
                     /locus_tag="ACG8ZM_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493679.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG8ZM_002773"
     CDS             588..1004
                     /locus_tag="ACG8ZM_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493680.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG8ZM_002774"
     CDS             complement(1141..1860)
                     /locus_tag="ACG8ZM_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0493681.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACG8ZM_002775"
     CDS             2544..2630
                     /locus_tag="ACG8ZM_002775"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0493682.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACG8ZM_002776"
     CDS             complement(2932..>3221)
                     /locus_tag="ACG8ZM_002776"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493683.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAZKOR010000162         3083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000162 DAZKOR010000000
VERSION     DAZKOR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.168"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(550..2163)
                     /locus_tag="ACG8ZM_002777"
     CDS             complement(550..2163)
                     /locus_tag="ACG8ZM_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0493684.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2304..>3083)
                     /locus_tag="ACG8ZM_002778"
     CDS             complement(2304..>3083)
                     /locus_tag="ACG8ZM_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0493685.1"
                     /translation="LYAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFY
                     FDEQLLAHITGQSFSIDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQ
                     QMSIHLLSLLVTYCLQKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDP
                     IIRYLEEHLSENFLLEQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVL
                     ENELTYTEIADILGFHSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT          996 a          540 c          538 g         1009 t
ORIGIN      
        1 aaataaatag gaacaaaaaa attaaattat ttaatacaca cattagggat cattcattga
       61 aggattagaa gacaacgagg atggcgaaaa atatgattac aatagaaaag tcgttctaaa
      121 ccaatcagtt cctagtatca cacaacgcaa gccagtcgat ttggtgagat tagaggagtg
      181 aaagcaaact ttattatata agaaaaaata ttcgaaaggg atgctagttt aggtactagt
      241 gctgatagat taatatgatg cctcgtaata ttaggcgtag ttactctaga gacatcacta
      301 tggactatga tttgtctcag agtcacaaca tgttttttta tgaaaagaaa cagctaaaat
      361 aattaaattg cgcaatgttt aaactttctt aaactataac ctaataaatg tgaagaaata
      421 atgcacaatt tcttcacatt tatcttctat tctatataca taccaaccaa caaccctata
      481 tactttttca tttttactcc tcaagtaaaa ataactccta gaccgcggct acccaccgcg
      541 gtcgtttttt tattcttcaa tcaaaatcgc tttgataccc aaacctgcca agattcgggc
      601 agaagtataa gtattcaatc ctcttctgaa agtaatgtaa acttttttat cttttgggat
      661 ttcattgatt cgatctcgta actcattcat tggaatattt tttgttgccg agatactgcc
      721 agaaggtgct ttattaggtt cacgtatatc taaaaagaac gcttctttca aatcgttttc
      781 gggtatatca cttgctttga tcgtttctac gatattcgtc atttgattga ttgcgacata
      841 accagctata ttcaacgggt cacgggtagt agaatacggt ggagagtaag gcaactccaa
      901 atcaggcaaa tcaaagactg tcaagtttcc ggtgattgct acagaaagtt ctcccatccg
      961 tttatcgaca cctttttctc caaatgcttg tccacctaaa atacgaccgc tatctgcatc
     1021 aaaaatcagc tttagattta atcttgtagc ttctggatag aaataggcat ggtcaaaggg
     1081 agtgataaaa acagttttat aatttgttat gcctgatgct tttaattcat gttctgtcat
     1141 tcccgcataa cttgctgtat gatcaaagat ttttgcgaca cctgcaccaa tatatccgcg
     1201 atagcgcata ggcgtaccat ttaaaatatc tgccagcata tgtccttgac gattggccgc
     1261 actggaaagc atactttgga tcggctgacc agtcactaca ctagtcgttt cgataatatc
     1321 gccaatcgca tagatgtccg gcagattggt ttgtaactgg tcatcaacaa tgatctgacc
     1381 tgtatctgat aattgtatgc cggttgcttt caccacttca ttcttcggag aaacaccaac
     1441 agcaaaaatt agcatatcag cagaaaggac ggagccatct gataatacta tttctcgacc
     1501 tttatcacgg atctcagtaa ctcttgtttc taaatgaacg gctaatcctt ctttcagcaa
     1561 tttgtcataa actaaattag caatttctgg atcataaggg aaagcgactt gtggcaattg
     1621 atcgattaaa gtcacgtcta agccgcgatg cttgaaattc tcagctaatt ccagacccat
     1681 aacacctgca ccaagaatga cgacatgttg tggatttttt tcttcaagga acgatttgat
     1741 tcgatccgca tccgtcacac tacggaggac aaagccgttt tctgcttcaa aaactccggg
     1801 aatatccgga taatctggtc tagcaccaga agagatgatc aacttatcgt aatctgtttt
     1861 tatttcttca tttgtggata ggtctttaac tgttaatcgc ttagtagaag gatcgattgc
     1921 tgttacttcg tgtttagtaa agacatcgat gttgtttttt gttttcaata tttctggtgt
     1981 acgttcgatc agcgagtcac ggtcagtgat cacacctcct aaataataag gcaaagcaca
     2041 acttgcataa gaaatatttt ctccgcgttc atagataatg atttcgtgcg cttcattcaa
     2101 tcgacggaaa cgagtggcaa atgaaggacc gccagctaca ccgccaatga caacaacttt
     2161 cataaaaaca tctccttttc tacgttgatc gtactatgtt atggtaaaaa acagcaaata
     2221 attgtgccgg aaaattattg tttattttga atagacagaa aatatagaga aataatttgt
     2281 ggataaaacc taaaaatttt tttctatttt aaggatagat aatactttga aggggataac
     2341 cctgtatatt ttttaaaagc tgaagaaaaa tgatggatcg aatgaaatcc taatatatct
     2401 gcaatttctg tataggttaa ttcattttct agcacgagac gttttgcttt ttccattttt
     2461 agttctttaa aatagctaat aacacttttt ccattcactt ttctaaaatg tttttttaag
     2521 ctggctggac tgataaaaaa aatttgggct aattgttcaa gcaaaaaatt ttctgaaaga
     2581 tgttcttcta aatatcgtat gattggatca actaattcat ctgaataaag aatatgcttt
     2641 tttttaggaa tgactttctc atttgaaggt attgtcaata aagcaagttc ctgttgataa
     2701 tgccttggta aggaagacaa ttgaattttt tgcagacaat aagtaactaa taaggaaagc
     2761 aaatgaatgc tcatctgttg gcatagtatg aaaaaagaat tgatttcctg atcataggaa
     2821 atagctggct tactatgttt tagataatca attttgaact gttcattttt cagaaaagta
     2881 atgatagatg ttatttcttg atctatagaa aaagattgtc ccgtaatatg tgctaataat
     2941 tgctcatcaa aataaaactg gataatgata aaagtttgtg tttccttttg agtagaaaag
     3001 atttggactt cttgctgttt atcaatgaat aaaagggaag gattatctag ttctatagat
     3061 ttatgtgcaa attcagcata taa
//
LOCUS       DAZKOR010000163         3040 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000163 DAZKOR010000000
VERSION     DAZKOR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.169"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG8ZM_002779"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="ACG8ZM_002779"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0493686.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="ACG8ZM_002780"
     CDS             complement(1036..1956)
                     /locus_tag="ACG8ZM_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0493687.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /locus_tag="ACG8ZM_002781"
     CDS             2069..2821
                     /locus_tag="ACG8ZM_002781"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0493688.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          856 a          612 c          526 g         1046 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccgcagat
//
LOCUS       DAZKOR010000164         2829 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000164 DAZKOR010000000
VERSION     DAZKOR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.170"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..971
                     /locus_tag="ACG8ZM_002782"
     CDS             84..971
                     /locus_tag="ACG8ZM_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0493689.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1111..2037
                     /gene="cysK"
                     /locus_tag="ACG8ZM_002783"
     CDS             1111..2037
                     /gene="cysK"
                     /locus_tag="ACG8ZM_002783"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0493690.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2273..2719
                     /locus_tag="ACG8ZM_002784"
     CDS             2273..2719
                     /locus_tag="ACG8ZM_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0493691.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          948 a          454 c          612 g          815 t
ORIGIN      
        1 attaaaagta taatttaatt aattaagttt gttttaagaa gagaactttt tattaagttt
       61 ttttattttg aggaggaatt tgaatgcaag agctgaagac ccgcagtgcc cggcaccata
      121 gacctaaaag aaaaatggtg atcaataaaa aagcgattgc tcctttaatt ggaacgagtc
      181 ttttagttgg gccgatgatg gttacagcgg taccgcaaca tgttcatgca gatgaacaag
      241 gatctcaaac agtcgctgat cctcaagcgt ttatcgatca aatcggctgg tctgcgtcag
      301 aagttgctgc aagtaatgac ttgtatgctt cagtcatgat cgctcaagcg ttgttggaaa
      361 gtgcatatgg cacttccgga ttagctagtg cgccaaatta taatctattc ggtgtgaaag
      421 gaagctataa cggacaggtc gtttatatgg caaccaaaga atatgtaaac ggacaatggg
      481 taacgatgaa tgagccattc cgaaaatatt cttcttattg ggaatccttc caagaccatg
      541 cgaatgttct tcgttcaacc agtttttcca cgggcgatta tcattatgct ggcgtgtgga
      601 aaagcaatac aacaagtttt tatgatgcaa cagcgtactt gacaggtcgt tatgctaccg
      661 atccaaatta tggggccaaa ttgaactcat tgattaatac ttataattta acccgttttg
      721 atacgccatc aacaggttcg gctacaacaa ccacaagtac aacaacaacg actactacga
      781 cgacttcaac gtcatcatct tcaacaacag atgtaaatac aaataatggt cagacatata
      841 cagtcgtgtc aggtgatacg ctatgggata ttgcacaaaa atttggcatg tctgttgatg
      901 atctaatggc gaaaaatggc atgtctatgt ccagttattc tttattagtt ggtcaagaac
      961 taaacgtttg atttagataa aaaggcaaag aaattaccat atatggtata tctttgtctt
     1021 ttttttgcta gtcaaagacg aatcactcgc cataaatgaa cagattgtat tatgataagt
     1081 aagaagagca aagacaagga ggaattgaag atgacagcta tctataactc agtcaccgaa
     1141 ttgattggca aaacgccaat cgtcaaatta aataaaatcg taccagaaga ttcagcagat
     1201 gtttttgtaa agcttgagtt ttttaatcca ggaggaagtg taaaagatcg tattgcttta
     1261 agtatgatag aaaaagcaga acacgatggt ctactgaaac caggtgatac tatcattgaa
     1321 cctacttctg gaaatacagg aatcggattg tcgatggtag gagttgcaaa aggatataaa
     1381 gtaatcatcg tgatgcctga gacgatgagt atcgaaagac gtttattgat gaaagggtac
     1441 ggcgcagagt tgattttgac tcctggtgcg gatggtattt ctggatcaat cagagaagca
     1501 gaacgtttgg caaaagaaaa cggctacttt ttaccattac aatttgaaaa tgaagcaaat
     1561 cctcttgtgc atgagaagac gactggtcct gagatacatc aagcatttgg agtaaatggc
     1621 ttggacgcgt ttgttgcagg tatcggtaca ggaggaacga tcactggcgc tggccgagaa
     1681 ttaaaacgtg tgtatccgaa aatcgaatta atcggggtag aaccagcaga atctgctatt
     1741 ttagaaggga aagaagcggg acctcacaaa atccaaggaa tcggtacagg ttttgttcct
     1801 aaaacactgg atacttctgt gtatgataaa gtcctttcta ttagtggaga cgaagcgatg
     1861 gaaacagcta gagaagttgg gcgaaaagaa gggattttag taggcatctc atctggcgct
     1921 gcaattgctg ccgcgctaaa agtcgcaaaa gaattaggaa aaggcaagaa ggtattagct
     1981 gtggttcccg acaatggtga acgttattta tctacagccc tttaccaaga gatataaggc
     2041 acttggtttt tctccagata ggtttatgta aaaaactttt aaagatgaaa agaacacaca
     2101 aaaatttatg atgagaaaaa ttatttgaat agagacgtag aatagtgaac gcgactgttt
     2161 tacgtctttt ttcattaaaa tacaataaaa ttgaattttc ttttcatgtt aagtggattt
     2221 taaaatgtaa aaatagtatg ataagacgag aataacaacg tggggtgttt acatggaaaa
     2281 taagttagtc gaagaggtta tcgaaaagtt aaaaaaagag aacattcgaa tcacacctca
     2341 acggtacgcg gttttagaat atttgattga acatcattcc catccaacgg ctgatgagat
     2401 ttataaagca cttgaacatc ggtttccaaa tatgagtgtt gcgactgttt acaataacct
     2461 tcgcttattt acttcgattg gatttgttca agaaatgaag tacggcgatg cttctagccg
     2521 atttgatttc agttcaaagc gtcattatca tgtaatttgt gaaaactgcg gtaagattgt
     2581 tgattttcat tacccagggc ttgaagatat agaaatggct gcaagcagac tgactgattt
     2641 tgagattaat gaacatcggt tggaattgta tggactctgt ccggattgta aagagtcagc
     2701 aaaaaaggcg caaatgtaaa aacagtttct acaagtgtta tacctaaaaa aagctagctg
     2761 tgtaaaaaaa acgctagctt ttttattttt ttgtctttta ttcacaatct tagatataaa
     2821 agtgaatat
//
LOCUS       DAZKOR010000165         2796 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000165 DAZKOR010000000
VERSION     DAZKOR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.171"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACG8ZM_002785"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACG8ZM_002785"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0493692.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1085 a          435 c          569 g          707 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattgg
//
LOCUS       DAZKOR010000166         2683 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000166 DAZKOR010000000
VERSION     DAZKOR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.172"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..179
                     /locus_tag="ACG8ZM_002786"
     CDS             <1..179
                     /locus_tag="ACG8ZM_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0493693.1"
                     /translation="EDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFASSL
                     MLIKKPKEILSKIS"
     gene            189..845
                     /locus_tag="ACG8ZM_002787"
     CDS             189..845
                     /locus_tag="ACG8ZM_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0493694.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            845..2221
                     /locus_tag="ACG8ZM_002788"
     CDS             845..2221
                     /locus_tag="ACG8ZM_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0493695.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            2351..>2683
                     /locus_tag="ACG8ZM_002789"
     CDS             2351..>2683
                     /locus_tag="ACG8ZM_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0493696.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1060 a          318 c          477 g          828 t
ORIGIN      
        1 cagaggattc aatgatttcc ggtggaagtt taataaataa tagtagtttt atgtcaaata
       61 taacaacact tggacaaagt tatttaatat taataagtat tattgtttta tcagttgtat
      121 ttgcttcttc attaatgtta atcaagaagc ctaaagaaat cttatcaaaa ataagttagg
      181 agaaaataat ggatatatta gaaataaaga atgtagcata tagttatgca aattctaaag
      241 aaaaagtttt gtcaggagta aatcaaaaat ttgaacttgg gaagttttat gcgatagtag
      301 gaaagtcagg aacaggaaaa tctacacttc tttccttact tgctggactt gataaacctc
      361 aaacaggaaa aatattgttt aagaatgaag atatacaaaa taaaggatat agtaatcata
      421 gaaaaaataa tatatcttta gtatttcaaa actataattt aatagattat ttatcaccaa
      481 ttgaaaatat tagattagta aataaatcag cagatgaaag tatcttgttt gaactaggtt
      541 tagataaaaa acaaataaaa agaaatgtta tgaaattatc tggtggacag caacaaagag
      601 tagctattgc tagagcattg gtatcagatg ctccaataat actagctgat gagcctactg
      661 gtaatctaga cagtgttact gctggagaaa taattaatat attaaagaca ttagctaaag
      721 atagaaataa atgtgtaata gttgtaacac atagtaagga agtagcagat tctgcggata
      781 tcattttaga actaagtggt aaaaagttga aaaaagtaaa taaaatgaat ttggaggttg
      841 aataatgata aaaaatgcat ttgcttatgt aactagaaaa agtttaaaat cattaattat
      901 catattagtt attttatcta tgtcaacttt aagtctcatt agtttatcta ttaaagatgc
      961 tacggacaga gcttcaaaag aaacatttgc taatataaca aatagttttt ctatggagat
     1021 aaatagacaa gtaaatccag gaacacctag aggtggggga aatgtaaaag gtgaagatat
     1081 taaaaagata tctcaaacag atagtataga ctcctatgta aaaagaataa acagtgttgc
     1141 agatttagtt gattatgata ttattgaaac tcaagagact cttgcgaatc aatcacctga
     1201 aagagcaaag aattttaaaa gaacagttat gttaactggg gttaacgact caacaaaaga
     1261 aacaaaattt gtatcagaag catataaatt agtagaagga aagcatttag aaaatgaaga
     1321 taaaaataaa atcttaatgc ataaagattt agctgaaaaa aataacctta aagttggaga
     1381 taagataaaa ataaaatcta atttatttga tgctgataat gagaaaggtg cagatgaaac
     1441 agtagaagta gaaattaaag gtctattcga tgggcataat agcggtggag taagtgcagc
     1501 acaagaacta tacgaaaata cattaatcac tgatattcat acagctgcta aagtttatgg
     1561 taataccgaa gatacagctg tataccaaga tgcaacattc tttgtaaaag gtgataagaa
     1621 tcttgatagt gtaataaaag atcttgggaa attagatata aattggagag aatataattt
     1681 gattaaaagt tcatcaaatt accctgcatt acaacaatct atatcaggta tttactcaat
     1741 ttcaaataaa ttattttttg gctcattaat atttgcagga gttgtagttt cattattatt
     1801 attcttatgg atgaatgcta gaaagaaaga aatagcagta ttactatcat taggtatatc
     1861 aaaattagaa atttttggtc aattcctaat tgagatggta tttatatcaa tccccgcatt
     1921 cgttggttcc tatttcttag ctcaatatac agctgacaag cttgggaata atatattgaa
     1981 taaggttact ggtgatatag ctaaacaaat agctaaacaa tctgcatcta gccaattagg
     2041 tggtggggca gaagctgaag gatttaacaa gacattatca agtttagata tcaatgtatt
     2101 acctaaattc atgatttatg tagttatatt tatgagttta gtacttcttg tatcattgat
     2161 tatttcttca ttcaatatat taagaaggaa tccaaaagaa ttattaattg acaataatta
     2221 aaattttcgg tgctttttag caccaaaaat gagagtgtaa tataaaccgt gtaagtaacc
     2281 atcaatccta ggcgggtggt tgcttcacgg tttttatttt tctaaaataa gatcaagagg
     2341 tgattacacg atggctagaa agaaaagaaa tcctgatgct gagaagttag ctgaatccat
     2401 tttgaatact tatcaacccg aatctgtaga ggatatgcaa gatgttttaa aagatgtgtt
     2461 tggtccctta tttgaaaaaa tgcttcaagg agaattgaat aaccatttag ggtatgacgc
     2521 ccattctaaa gagccaaaga aacatgacaa tcgacgaaac ggttatggca ataagacgct
     2581 taaaacgagc tttggtaaag taaacattga tgttcccaga gaccgggagg cttcttttga
     2641 acctgagttg attcctaata gaaaacgaga tgtctccgac atc
//
LOCUS       DAZKOR010000167         2601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000167 DAZKOR010000000
VERSION     DAZKOR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.173"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(120..995)
                     /locus_tag="ACG8ZM_002790"
     CDS             complement(120..995)
                     /locus_tag="ACG8ZM_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0493697.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(1250..1334)
                     /locus_tag="ACG8ZM_002791"
     tRNA            complement(1250..1334)
                     /locus_tag="ACG8ZM_002791"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1298..1300),aa:Leu,seq:caa)
     gene            complement(1352..1423)
                     /locus_tag="ACG8ZM_002792"
     tRNA            complement(1352..1423)
                     /locus_tag="ACG8ZM_002792"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1389..1391),aa:Gln,seq:ttg)
     gene            complement(1433..1505)
                     /locus_tag="ACG8ZM_002793"
     tRNA            complement(1433..1505)
                     /locus_tag="ACG8ZM_002793"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1470..1472),aa:His,seq:gtg)
     gene            complement(1567..1651)
                     /locus_tag="ACG8ZM_002794"
     tRNA            complement(1567..1651)
                     /locus_tag="ACG8ZM_002794"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1615..1617),aa:Leu,seq:caa)
     gene            complement(1672..1742)
                     /locus_tag="ACG8ZM_002795"
     tRNA            complement(1672..1742)
                     /locus_tag="ACG8ZM_002795"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1708..1710),aa:Cys,seq:gca)
     gene            complement(1788..1859)
                     /locus_tag="ACG8ZM_002796"
     tRNA            complement(1788..1859)
                     /locus_tag="ACG8ZM_002796"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1825..1827),aa:Gln,seq:ttg)
     gene            complement(1871..1943)
                     /locus_tag="ACG8ZM_002797"
     tRNA            complement(1871..1943)
                     /locus_tag="ACG8ZM_002797"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1908..1910),aa:His,seq:gtg)
     gene            complement(1946..2019)
                     /locus_tag="ACG8ZM_002798"
     tRNA            complement(1946..2019)
                     /locus_tag="ACG8ZM_002798"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1985..1987),aa:Trp,seq:cca)
     gene            complement(2026..2106)
                     /locus_tag="ACG8ZM_002799"
     tRNA            complement(2026..2106)
                     /locus_tag="ACG8ZM_002799"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2070..2072),aa:Tyr,seq:gta)
     gene            complement(2108..2183)
                     /locus_tag="ACG8ZM_002800"
     tRNA            complement(2108..2183)
                     /locus_tag="ACG8ZM_002800"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2148..2150),aa:Phe,seq:gaa)
     gene            complement(2219..2291)
                     /locus_tag="ACG8ZM_002801"
     tRNA            complement(2219..2291)
                     /locus_tag="ACG8ZM_002801"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2256..2258),aa:Asp,seq:gtc)
     gene            complement(2301..2373)
                     /locus_tag="ACG8ZM_002802"
     tRNA            complement(2301..2373)
                     /locus_tag="ACG8ZM_002802"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2338..2340),aa:Val,seq:tac)
     gene            complement(2389..2460)
                     /locus_tag="ACG8ZM_002803"
     tRNA            complement(2389..2460)
                     /locus_tag="ACG8ZM_002803"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2425..2427),aa:Glu,seq:ttc)
     gene            complement(2475..2564)
                     /locus_tag="ACG8ZM_002804"
     tRNA            complement(2475..2564)
                     /locus_tag="ACG8ZM_002804"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2528..2530),aa:Ser,seq:gga)
BASE COUNT          774 a          597 c          483 g          747 t
ORIGIN      
        1 aaatcacttt tcccatttca ttctccttct tttttctact aaaataggcg caaccaaata
       61 atcatagtcc atttactatg tgtcatttgg ttgcgcctat agatagttac gcccttattt
      121 taatagattc ttgctagatg aattgccaaa tagatgattt cattttcttg aatggcaagt
      181 tgaaattgct gttcgatgat atctttaatc tttagtgctg tacaatacgc tttgctatgt
      241 ttaaatgttt cagtaaaaaa accaatattt tctactggcg tttcttcctg ataaatcaag
      301 cgtttaacca ggaatttgat atgaaccatt aagcgatgat aagtcagtga ttctttatca
      361 taaacgtttg aataggattt ttctagatgg gacaaaatcg tggtcgtcat ttctactatt
      421 tttaagctct ttaaactatt aggctctcct agctcagcat taatgatatg catagtgatg
      481 aatcctactt catttgaccc taactcggtt tgatatcgat gattgattcg ctcgaccact
      541 tctactgcta tatcatattc atttggataa aggaatctca tttctggcaa cataattgct
      601 gtgacatctc tatcttctct ttttcgttga atagcaaaat aaatatgatc tgtcaatgta
      661 atatagatat ttgaatctaa tggagaggag atcctagctg ttatcagttc aatcacttct
      721 tccgcaaaat ccataaattc gattggaatc tgcgagacta tctcactgaa tcgtttgatc
      781 ttttctttag aatctaatac aaataatttg tcagcatcct caacatcaaa tggcttactc
      841 actttagcag aaaagccaat ccctttcccc atcgcaattt tttctattcc atcgtcatcg
      901 atcaatacag cattatgatt cagctttttc atcacataca ccgttaattc acctcctcta
      961 tatgataaaa caaaacaact tgcagactcc ttcatacata tttttgtctc caaacaattt
     1021 ttaaagacaa aaaacgacct aagaaagaac cctttcttag gtcgtgccta attgaatagt
     1081 agcaccctac ataatttaca aaaatagttt aacacataat aaaagcgtta tcaatatctt
     1141 ttttactcgt tttatgataa tacttgtatc gaattgattt ggaggtaatt acataaaaac
     1201 aaaaaaaata tagcaactct ctataagaaa gttgctatat tgataattat accggcggcc
     1261 ggggtcgaac cggcacgtcc tcaacggaca ctggattttg agtccagcgc gtctgccaat
     1321 tccgccacgc cggcaaattt aaaaataata actggggtag ctggattcga accaacgcat
     1381 gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa agggcgaatg
     1441 atgggaatcg aacccacgaa tgccggagcc acaatccggt gcgttaacca cttcgccaca
     1501 ttcgccatca taaaagatgt tatttaattt ctcaataaaa aactaagatg aagacatctt
     1561 agtcaatacc ggcggccggg gtcgaaccgg cacgccctca acgggcactg gattttgagt
     1621 ccagcgcgtc tgccaattcc gccacgccgg caagttgatt tattagtact aaggcggtaa
     1681 ccggatttga accggtgatg aaggttttgc agacctctgc cttaccactt ggctataccg
     1741 ccattatact attatcaatc tataaaataa agaataaatt atagaaactg gggtagctgg
     1801 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
     1861 gaataaaaag ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc
     1921 gttaaccact tcgccacatt cgccatggca ggggcagcag gaattgaacc cacactaacg
     1981 gttttggaga ccgctgttct acctttaaac tatgccccta taaaatggag gagagtggat
     2041 tcgaaccact gaaccctaag gaacggattt acagtccgtc gcgtttagcc acttcgctac
     2101 tcctccatgg tggcgcggga cagaatcgaa ctgccgacac acggagcttc aatccgttgc
     2161 tctaccaact gagctaccgc gccatatttt aaaattatta attgaataac aagaacgacg
     2221 gttccgacgg gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt taacccctac
     2281 accacggaac cagttcatta tggaggttaa cgggatcgaa ccgctgaccc tctgcttgta
     2341 aggcagatgc tctcccagct gagctaaacc tccaacattc aattaaaatg acccgtacgg
     2401 gattcgaacc cgtgttaccg ccgtgaaagg gcggtgtctt aaccgcttga ccaacgggcc
     2461 aggtaaattt gctacggaga gtaagggatt cgaacccttg agacagtttg gcaccatcta
     2521 catgatttcc aatcatgctc cttcggcctc tcggacaact ctccagagtg aaagaagctc
     2581 aatactcttt ctcaaaatga g
//
LOCUS       DAZKOR010000168         2594 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000168 DAZKOR010000000
VERSION     DAZKOR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.174"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..1059
                     /locus_tag="ACG8ZM_002805"
     CDS             52..1059
                     /locus_tag="ACG8ZM_002805"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFQ0493698.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1071..2075
                     /gene="neuB"
                     /locus_tag="ACG8ZM_002806"
     CDS             1071..2075
                     /gene="neuB"
                     /locus_tag="ACG8ZM_002806"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFQ0493699.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2072..>2594
                     /locus_tag="ACG8ZM_002807"
     CDS             2072..>2594
                     /locus_tag="ACG8ZM_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0493700.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAP"
BASE COUNT          950 a          334 c          520 g          790 t
ORIGIN      
        1 aagattaaga atcgaattat agatgactat aattcatagg aggagatata agtgtttaaa
       61 ggaaaaacaa tattagtaac tggcggaact ggttccattg gaagtgaaat tgttcgtcag
      121 cttttaaaat atgaaccaaa agctatcaga atttatagta gaggtgaaga aaaacaattt
      181 tatatgcaac aagagctctc aaatcacact aatttgagat ttttaattgg tgatatcaga
      241 gactctcaaa ggattgatta tgcatgtcgt gatgtggata ttatttttca tgctgccgca
      301 atgaaacacg ttccagcaag tgaatataat cctatggaag ctgtaaaaac aaatgtgatg
      361 ggaactcaaa atgtaataga tgcagcgatc aaaaataatg taaaacattt tgtaggtatc
      421 agtacagata aagtagtgag tcctactaac accatgggtg cgacaaagtt attgagtgaa
      481 aaattaataa tagctgccaa tcagtataaa ggaaaaacag aaacaatatt ttcttgtgta
      541 aggtttggaa atgtcatggg gtcaagtgga tctgttattc ctctttttag taaacaaata
      601 aaaaacaata aagatattac tttgacagat gttgatatga cacgatttat gatgtcaata
      661 ccggatgctg caagattagt tctgtctgct gctatgaatt caaaatttgg agaaacatat
      721 gtcttaaaaa tgccggcaat caatatttta actttagctc aatcattaat aaaaatatat
      781 aatgatacac atgatgaaaa atatagaggt gaaataaaag ttgtaggtat caggccagga
      841 gaaaaattat atgaagagct aatgactaca gacgaaattg aacgctcata tgaaaatgat
      901 aaaatgtata tcattccacc acactatcat gctactgacg aactgtacaa agattatgaa
      961 aaaattaaaa tcactgcata ctcttcagat gcaacagaat taatgagtgg acaggaattt
     1021 attagaatcg caaaagagaa taatgtcgtg acttggtaag gagttttatg atgaaatcag
     1081 aagtatatgt aattgcagaa attggtgtaa accataatgg aagtctagat ttagctttag
     1141 aaagtattga taaagctaaa gaatgtggag ctaacgcggt taaatttcaa acatttaaaa
     1201 cgaataagct agtttccaaa aaggctccaa tggcaaaata tcagaaggat aatcttaaag
     1261 aggacagttc tcaatttgaa atgctaaaaa aattagaatt atctcaatct gattttatta
     1321 gaataaaaga ttattgtata caaaataaaa ttgatttttt gtctactcct tttgatgaag
     1381 aaagtgcaga ctttctaaaa caaattggtg tacaaggatt taaaattggc tcaggagatt
     1441 taacaaattt accttttctt agaaaaatag atatgtatgg gttgccagtt ttattatcta
     1501 caggtatggc tgatattcat gaggtcagtg aggctgtaga ttgtttcata gatagtccag
     1561 ttactatttt acattgtact tcaaattatc cggctagttc tgaagatatt aatttattgg
     1621 cgattaacac attaaaagaa atgttcggga tacccgttgg gtactccgat cattcattgg
     1681 gatatgatgt tggtatttgt gctgttgcga tgggtgctaa ggtaattgag aaacatttta
     1741 ccttggataa ggaactacct ggtccagatc ataaagcttc tttagatcct caggaatttg
     1801 cagattttgt aaagcatatt agaaacactg aaatattttt aggtgatggt gttaagcgag
     1861 caatgccttc tgaacttagt acaaaagaag tatcaagaaa aagcctagtg gtcactgaga
     1921 atttaaatgt gggagatatt cttacagaga aatctcttac aataaaacgt cctggaaatg
     1981 gaatagctcc taagttttta tatcaatata ttggcaaaaa agttaaaaga ccaataagtg
     2041 cagatagtgt tctgcttgag gaagatgttg aatgaaaaaa atacttatag taagtggaac
     2101 acgagctgat tatggaatat ataaacctgt tgctgagaat ttactacaag ataattggga
     2161 tgtatcattt gttgtttctg gaatgcattt atcccataaa tatggattta ctaaagaatt
     2221 gatcttagct gacggattta gaattagtgg agaagttcaa agtttgtttt tagagaacaa
     2281 taagggaaac atggctcggt ctgtggcatt agagataaat ggtttcacaa atatatttga
     2341 acaaactagc cctgattttg tattattact aggggataga ggagaaatgt tggcagctgc
     2401 gatagcttgt ttgtatcttg gaatcaaaac agcacatatt cacggaggcg aagtgtctgg
     2461 tactgtagat gaatcaataa gacatgccat aacgaaaata gcatctgttc attttgctgc
     2521 tacggaaaaa agcaaagagc gtttaataaa aatgggggaa gattcttgga gagtttttcc
     2581 cgtcggagct ccta
//
LOCUS       DAZKOR010000169         2499 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000169 DAZKOR010000000
VERSION     DAZKOR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.175"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(270..556)
                     /locus_tag="ACG8ZM_002808"
                     /pseudo
     CDS             complement(270..556)
                     /locus_tag="ACG8ZM_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(733..1911)
                     /locus_tag="ACG8ZM_002809"
                     /pseudo
     CDS             complement(733..1911)
                     /locus_tag="ACG8ZM_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1941..2351
                     /locus_tag="ACG8ZM_002810"
     CDS             1941..2351
                     /locus_tag="ACG8ZM_002810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493701.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPKK"
BASE COUNT          816 a          395 c          453 g          835 t
ORIGIN      
        1 aatgaaaaac gagaaggaag atgactttaa agtagtttag tatgaaggtc gattgataaa
       61 acgatgtgga ttaatgctaa ataatgagta atttttcaat aatacaaaaa ctgtgtacag
      121 tattagaagt agagaaacgc tagcgtcgaa aaaaagatat ttttcgtgaa aagtaatagg
      181 ggctaaaaag aaagtataca atttatttat gtttctaagc atataaaaaa gagcgttgaa
      241 aggaatattt ccccttcaac gcttcttttt tagaagattt taatgaccat ctcaacttca
      301 ttttcaggtt taggtaattc atggaagctt gtgtttatcg tgcttaattg atcaactaac
      361 catgttttac tttttgactc gtagacaaat ctaaaattaa tatcattgtt ctcgtgttga
      421 agaggtttct ccaaataatc tggtgaatat tcatagtcaa cgtaacctgc taatacaaaa
      481 gaaagcagtc cattatttag tgtcagattt aacggatggt cgtaagcttt tattttttta
      541 atgtcatatt gtacatctta tgaagactta atgactactt gtagtcaata gatttaaagt
      601 atttattttc tcatttatgt atcaagaata aatactgata taataaaaat tatatcaatg
      661 ttttgatgaa gtaatttgga gggagcgtca ataattttgt gtaaataact cgtccttctg
      721 caaaataatg ggttactcag taaacattga agctaatgta tccgtcactt gttggaagcc
      781 tttatggctt ctatttaaga atttttgatt gtatgtatca aagatggata ctaggaaacg
      841 ctctagtgat tcttcatttt gaaactgctc ttttctgcgg ctgtatttct tgatttgttt
      901 cttgaaagat tcgattaaat tagttgagta aatgcttcgg cggataccag gcggaaactc
      961 ataaaaagtc aataaatctt gattttttat cagtgactgc gtcactttag gataagtttt
     1021 ttgctacttc tcaatcatac tccctaaaaa ggtatttgcc tcttcctttg agctagcttg
     1081 ataaacagcc ttaaaatcat cacagatttc ttttcgatct ttgacacgta ctttatgagc
     1141 aatattacgg gaaacgtgaa cacagcaatg ttggtattta gctttaggat aaatctggtg
     1201 aatcgtatct ttcatgcctt ttaagccatc ggtaataaac agtaagacat catgaacccc
     1261 tctggagtaa atgtcctgta acaactcatt ccaaacgtag gtcgattcag tcggagcaat
     1321 cgcataactt agtacttctt tagtgccgtc ttctcgtata ccaatggcaa tatagattgc
     1381 ttctttggag acggtttgcc gttttaaagg aatataagta gcatccatga aaatagcgac
     1441 atacttatca tttagagttc taactttaaa tgcatttact tcttcagtca gcactttagt
     1501 catgttggac atggtttggg gcgtgtagtg atgcccatac attttctcaa tcaagtctgc
     1561 gatttccgac atggtaatac cttttcggaa gaggtgaatg acggtctcct ctaagatatc
     1621 attcgtccgt ctataagcag gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg
     1681 aatctggaga tgaagttccc cgtactcggt cttgaccgta cggtcatagg agccgttacg
     1741 agaattcccc gtgttaaaac caatgcgatc gtacttttcg taatcgagga aagccgttaa
     1801 ctccgttttg agaagcgtat tgacagcttt ttccaaatgg acacggaata attcattcaa
     1861 atcgcctttg ttcgctagag tctttagaat ttctgtagta aaatcgttca tatggaagtc
     1921 ctcttttctg tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta
     1981 tttacacaaa atattttaca ctctcgtcaa atataaaaac tagtacgaaa ttaatttgta
     2041 aattatttaa gcttgataaa ataagcaata tcgatgatgt aaagtccaag atattaacaa
     2101 gaaaagtaag cgaaccagta aagctaacgg cacaagataa aaaaaatgga attgttcttt
     2161 atggtgatga tgcaaagtat aactataatt gggatacctc ctcagaagct gaggtattaa
     2221 gtattcctac taccgatact tcaggggaaa aagtcacctt taaaataaat tttataacgg
     2281 caatttctaa gttggagtat gggaagcaaa aaaagagaga taacaatgat caatatctac
     2341 ctaaaaaata acgaattaat gatgcatatg ttaaagttgt ttttttgaat taaacaactc
     2401 aatttattca gttatatgtt catcgcagca agtgaacatt aatcgagtta taaaattaat
     2461 aggagctaaa aatataaata aagttcgatc agaatttat
//
LOCUS       DAZKOR010000170         2493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000170 DAZKOR010000000
VERSION     DAZKOR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.176"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG8ZM_002811"
     CDS             <1..435
                     /locus_tag="ACG8ZM_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0493702.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG8ZM_002812"
     tRNA            complement(759..842)
                     /locus_tag="ACG8ZM_002812"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG8ZM_002813"
     CDS             1370..1870
                     /locus_tag="ACG8ZM_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0493703.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG8ZM_002814"
     CDS             complement(2060..2404)
                     /locus_tag="ACG8ZM_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0493704.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT          777 a          478 c          413 g          825 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt tta
//
LOCUS       DAZKOR010000171         2490 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000171 DAZKOR010000000
VERSION     DAZKOR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.177"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..1657)
                     /locus_tag="ACG8ZM_002815"
     CDS             complement(365..1657)
                     /locus_tag="ACG8ZM_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0493705.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="ACG8ZM_002816"
     CDS             complement(1931..2191)
                     /locus_tag="ACG8ZM_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493706.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          844 a          465 c          381 g          800 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
     2341 gtaaaatatt ttgtgtaaat gaaaaaatcc atacaaaaaa ggaaggcgct tctgtagaat
     2401 aaagttaacg acaaccaatt cacagaaaag aggacttccc tatgaatgat tttactacag
     2461 aaattgtgca aactctagtc actaaaggcg
//
LOCUS       DAZKOR010000172         2390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000172 DAZKOR010000000
VERSION     DAZKOR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.178"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2059
                     /locus_tag="ACG8ZM_002817"
     CDS             197..2059
                     /locus_tag="ACG8ZM_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0493707.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2390
                     /locus_tag="ACG8ZM_002818"
     CDS             2059..>2390
                     /locus_tag="ACG8ZM_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0493708.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          860 a          353 c          465 g          712 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAZKOR010000173         2321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000173 DAZKOR010000000
VERSION     DAZKOR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.179"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            18..2171
                     /locus_tag="ACG8ZM_002819"
     CDS             18..2171
                     /locus_tag="ACG8ZM_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0493709.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          796 a          414 c          519 g          592 t
ORIGIN      
        1 aggcggaagg ataggaaatg atgaaagatt ttatgaaaaa aatattaaat acttcttggt
       61 tagaaaaaat aagaaaaggt gcacaagaac ctaagaacaa aacgtttcgc cggtcccatg
      121 ttccttttcg cttaaatttt ctttttttca ttatatttac gttatttgtt gcgttgattg
      181 ctagattggg ttatttgcag atcgtgaacg gcgaagagat ggaggcgcga gtgaagtcta
      241 cttctacgat tacggtccaa gaaagtactc ctcgcggaat gatctacgac tccacaggaa
      301 aagctttagt tacgaataaa gcgaatcagg ccatcacata tacgagagga acacaagtta
      361 cggctaaaga tttgcttgaa atcgcaacga agctgaactc gctgatcgat gttccggtag
      421 atgaaaatct gacagataga gataaaaaag atttttggct ggctgatccc aaacatttga
      481 aagaagccac aaaaagactt tcggcaaaag aaaagcaatt agggaacagc gaacaatata
      541 gtgcgatggt caataaagtc acagctgatg agattaattt aaacgaggag caattaaaaa
      601 ttgctaccat ctttaaacgg atgaatagtg catatgaatt aaacactgtc tatatcaaaa
      661 actctggtgt tactgatcaa gagttagctg ttgtggcaga acgcgcttct gaacttccag
      721 gagtttcgac tggcacagac tggacacgag aatataatgc agcaagttca ctgaaaagta
      781 ttcttggcag tgtgacgact gaaaagcaag gacttccagc agatgaagca gagaaatacc
      841 tagcaaaagg ttattctcgg aatgaccgtg taggacaaag ttatttggaa aaacaatatg
      901 aagatgtcct gcaaggaacc aaaacccaat atgaagtttc ccttgataat gaaggaaatg
      961 tttcgaatca aaaagaaatc ttctctgggg aaaaaggttc taatttaatg ttatctatga
     1021 atgcagaatt ccaatcaaaa gtagaagaaa ttttgaaaag gaattatcaa acattgatca
     1081 acaacggcaa agcacagtat tcgcctggtg cttatgcagt ggctatgaat cctcagacag
     1141 gagaagtgct agcaatgact ggtttcagtc atgaacaagg ttccaaagaa atcaccgaga
     1201 atgctttagg cacgattact agcgcctttg atccagggtc tgtagtcaag gctggtactt
     1261 tgacagcagg ctgggcaagt aacgcgatca gcggaaatca agttttgatt gatgagccga
     1321 ttcgtctgca aggtgcttca gaaaaaagct ctgtctttaa tcgaagcgga caagttgctc
     1381 ttgatgcagt caaagcatcg gaattgtctt ctaatacgta tatgatcaaa gtggcgttaa
     1441 agatgttagg actagactat acacctggaa tgggactacc tagtttagat gaagaagcta
     1501 aagcttatca gcaattgaga gactctttca aagagtttgg tcttggtaca acgactggga
     1561 tcgatttgcc gaatgaatct cctgggatct cacgttctgt tgattatatg aagaagttca
     1621 atgcggataa tggcaaagaa tggtatgctc caggtaactt taccgatttg gcatttggac
     1681 agtttgacac atatacacca atccaattag ctcaatatgc ttcaacagtc gctaatggcg
     1741 ggaaacgagt acagccgcac ctagtcaaag cgatctatgg caatgacgaa aatggaaact
     1801 tgggtgaagt gaagaaagaa attggcacaa cagtagaaaa tacggtcaat atttctgcag
     1861 aaaatatggc gatcctccgt gaaggattcc accaagttgt ccatggtacc gatccttaca
     1921 caacagccaa accattagcc agtgcgaaaa tggacctttc cgcaaaaaca gggactgcag
     1981 aaacggttgc ggaaggtcat ccagatattg cgacagtcaa tagtaatatc gtggcatacg
     2041 gaccaacaga caatccggaa attgccatca gtgttgtctt gccgaactta ttagacgaac
     2101 aagaccacat aaacttgacg attgctaaag aaataatgga cgcgtattat gatatgttca
     2161 tggcaaaata aaaaagaggt cgtgaaaaag ccgcttagtt tcgagtatga agaaaaattt
     2221 tgtgaaaata gcttctcaca tttttgctta aatttgactt cataccgaga aactggcttt
     2281 tgaacaccgt ttactcggca aaaaaggttg tgacaaaaat t
//
LOCUS       DAZKOR010000174         2298 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000174 DAZKOR010000000
VERSION     DAZKOR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.180"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1043
                     /locus_tag="ACG8ZM_002820"
     CDS             <1..1043
                     /locus_tag="ACG8ZM_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFQ0493710.1"
                     /translation="AIQMKNTIFSDEYVEIIISDHSKNTKEIAKKIEEKKVFDGCHYL
                     ESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIY
                     ACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSYNFYCFF
                     PNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVG
                     EYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKI
                     FMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILK
                     NVKIAESLDDIL"
     gene            1040..>2298
                     /locus_tag="ACG8ZM_002821"
     CDS             1040..>2298
                     /locus_tag="ACG8ZM_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFQ0493711.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWG"
BASE COUNT          823 a          271 c          407 g          797 t
ORIGIN      
        1 tagcaataca aatgaaaaat acaatttttt ccgatgagta tgttgaaatt attatttctg
       61 atcattctaa aaacactaaa gaaattgcga aaaaaataga agaaaaaaaa gtgtttgatg
      121 gctgtcatta tttagaaagc aaatcttttg atgataatat tgggtatttt caaaagatta
      181 aggactgtct tcagatttct tgtggtaaat caaatcgata tgcctattat ttgaaaaact
      241 gtaaaagtga atattttgat gaatttattt gtttttcaat gggagaggtt tggttggaag
      301 gaatatatgc ttgcttatat cgaaataata aaaatgtaca ggtatcaatt ttagaagaag
      361 gtattttaag ctatggagtt gacattattc caacagcaag aacaaaagct atcgcagtac
      421 tcaattactt aaaaaaaagg actccactat ataacaaaag ctataatttt tattgttttt
      481 ttcctaatat atatacagga agagagaata caatacaaat tccattagtt acagctgggt
      541 caagaaccgc attgcaactt tcggatattt ttaaccctat gatagatatt tataaagaaa
      601 aaaaatatat attttttacg tctgtttatg attttgaggg cggagaacct gtcggagaat
      661 atgaattagt atgcaaaata gcagacttag taggaaaaga taatctaatt gttaagacgc
      721 atcctagaga tgtaagaaca atttataaag ataatggatt tatcgaggat aaaaattcat
      781 caataccttg ggaggcgatt caattgtcag gcgattttaa cgataaaata tttatgacaa
      841 ttaattcagg aagtgttatt tcaggtagta caatgtctgc aaaacctgta cgcacgtatt
      901 atatgtataa attgtgtaac atagaaggaa atccgtcttg caaaaagaat gcgtatgata
      961 ttgagcgatt gcttaagcaa aaaacgatga aaaacatttt aaagaatgtg aaaatagcag
     1021 aaagcttaga tgatatacta tgagcaaaac taaatattta attaaaaata tgggaatatt
     1081 aacaataagt aatttttctt caaaaatttt agtttttttg ttagtccctt tatatacaag
     1141 tgtattatcc acaacagaat atggcacata tgatttgaca atttcaacag ccacattatt
     1201 gtttccaatt ttaaccttaa acattgtaga tgctgtgatg cgctttttaa tggataaaga
     1261 taaaaataaa aaaaatatag catctatagc gattaaagtt atatttttaa gcgtgatagt
     1321 ctttggatcc gtaatgtatt tgttaaatat tacaaaatta tggcaagatg tttatggatt
     1381 agaaaaatat atttttttgt attatatatc ctatgtatgg aatcaatttc taatacaatt
     1441 tgctaaaggg ttagaaaaag ttaaagctat gggcatagca ggtattatca gcacaattgt
     1501 aacaataatt gccaatattt tcttcttgct tattctgaaa tggggattaa ttggtttttt
     1561 cattgcaaat attttatcac agattatttc agctacttat ctgtcagtat tagtgagaat
     1621 atgggaatat atcgattttg aggctaaaga tcctcagcta aagagagaaa tgctaaatta
     1681 ctcgattcca ttaattgcaa ctgttgttgg atggtggatt aatagtacag ctgataggta
     1741 tgtggtttcc tttatagtag gtgtctcggc gaatggctta ttgtctgtgg catataaaat
     1801 acctcaaata attaatactt tgcagggaat atttatacag gcctggcaaa tttctgcaat
     1861 taaagaatat ggagataatg agactgcaaa attttatgga aatacatttg ttatgattaa
     1921 tttgttaatg tgcataacct gttcaagtct tatcatatta actagaccgt tttcacatat
     1981 tctttatgcg aaagactttt atgaagcgtg gaaatatgtt ccatttttat taatagctaa
     2041 tgttttaaat tgtgcatcag gtctgttagg acctatctta tcagcaaaaa aagattctaa
     2101 atccatgatg tggtctgcaa tcataggggc attagttaat gtgattatga gtgtagtcct
     2161 tgtttataag ataggggttc aaggtgccac aatagccact gttatatgtt catatgtaat
     2221 atatgttgtt cgtaaacgag ctgtgggaaa agatattttt ataaagcatt atcatattgt
     2281 tgtggaatct tggggatt
//
LOCUS       DAZKOR010000175         2293 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000175 DAZKOR010000000
VERSION     DAZKOR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.181"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1598)
                     /locus_tag="ACG8ZM_002822"
     CDS             complement(51..1598)
                     /locus_tag="ACG8ZM_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0493712.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG8ZM_002823"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="ACG8ZM_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0493713.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="ACG8ZM_002824"
     CDS             complement(2043..2237)
                     /locus_tag="ACG8ZM_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493714.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          477 c          408 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DAZKOR010000176         2232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000176 DAZKOR010000000
VERSION     DAZKOR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.182"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG8ZM_002825"
     CDS             <1..378
                     /locus_tag="ACG8ZM_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0493715.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG8ZM_002826"
     CDS             426..1826
                     /locus_tag="ACG8ZM_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0493716.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..2026
                     /locus_tag="ACG8ZM_002827"
     CDS             1859..2026
                     /locus_tag="ACG8ZM_002827"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493717.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2080..>2232
                     /locus_tag="ACG8ZM_002828"
     CDS             2080..>2232
                     /locus_tag="ACG8ZM_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0493718.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT          628 a          371 c          483 g          750 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttactggttc tgttgcaaag ttttaaatct actatcaaat
     2041 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag
     2101 caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc
     2161 tatcgtgaag ttcgagaaat cttatatgat cgtggcatta acgtttctca tacgacgatt
     2221 tatcgttggg tg
//
LOCUS       DAZKOR010000177         2189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000177 DAZKOR010000000
VERSION     DAZKOR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.183"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8ZM_002829"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8ZM_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(987..1952)
                     /locus_tag="ACG8ZM_002830"
     CDS             complement(987..1952)
                     /locus_tag="ACG8ZM_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0493719.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKOR010000178         2187 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000178 DAZKOR010000000
VERSION     DAZKOR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.184"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(224..1243)
                     /locus_tag="ACG8ZM_002831"
     CDS             complement(224..1243)
                     /locus_tag="ACG8ZM_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0493720.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1240..1464)
                     /locus_tag="ACG8ZM_002832"
     CDS             complement(1240..1464)
                     /locus_tag="ACG8ZM_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0493721.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1461..1754)
                     /locus_tag="ACG8ZM_002833"
     CDS             complement(1461..1754)
                     /locus_tag="ACG8ZM_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493722.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(1791..1928)
                     /locus_tag="ACG8ZM_002834"
     CDS             complement(1791..1928)
                     /locus_tag="ACG8ZM_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0493723.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(1930..>2187)
                     /locus_tag="ACG8ZM_002835"
     CDS             complement(1930..>2187)
                     /locus_tag="ACG8ZM_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493724.1"
                     /translation="KGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
BASE COUNT          604 a          451 c          366 g          766 t
ORIGIN      
        1 gagccgtctg ctctgaccaa ctgagctaag ggtactggtt gttgccacat aaagccataa
       61 acaatcaacc agtagaatgt gtggcaacaa acctgttatc gcatagcttg gagtgtgact
      121 attatgggtg atagtgaaga tatgcgataa catcactatt ttatcgaatg atttttatag
      181 ttgtcaatat agttatgtaa tgctcctcaa cgaggagcta tttttatcgt ttaggaatat
      241 ttaaatacca acgtttgtca tggaaatctt gcgcaccgcc tttagtgttt ccctctggat
      301 cattcgttgc ccgcatcatg acatagactt tcttattagg gaagttacgc atgttaaaag
      361 atacatgata gccaacattt ccagaagtat tataagcttg atttacatct gctctataaa
      421 ttccatcagc tcttactcga gctaattctt tcccagtatt gtaatccata atgaagatat
      481 actcgtattt atagttagca atgtgccatc cagctacatg caaatttgca ttttcgattt
      541 ctccaaactg atcaatgtgg gcgtaattcg ttccatctgt cagtgtagga ttagctgcac
      601 cagctcgtgt tggatcaatg acaggcttgt tttcagaagt tgttggattt tcatcggtaa
      661 atccatgagc taaatcatat gctaattttt ctttacttac gcccatttca gaaagataac
      721 cgtaaggatc tgtatgatcg ccccagatgt tttgtgttac ccataaatgc gatttgattc
      781 ccggttggtt ataaggagtg tccaacgtta atggaatacc atatttcatt gctgaatctc
      841 tagccaattc aacgtatgcc ttgtagtttt tctcaaacgt tgctttatca tgtgtgtgtt
      901 gtaactcaat ctgcacagga ctgttggcat tagcatacga accagcaccg tactgtacat
      961 aaccaggttg accgacttga taaacaattc cgccgtctcc cacaatataa gcagtataag
     1021 cgctagtcca tgaacgttgc atatactgcg cttcattgcg tcctgttgct gtttcattag
     1081 ccgtttcatg cagtaaaata tactgattat ttgctacttg tgagctacct tcatttgcgc
     1141 ccaaattaaa ttcattgttg atagtatagg caaatccgtt aattggcaat aaaaaaagag
     1201 ccgttaacag gctcatcgca gtaatagtaa ttttcttttt catttgtttc ctccttcttc
     1261 gctttcagcc gagaacattt tgtaggttcg atttgataca cccaacacac tccctaaaaa
     1321 cgcgccaaaa ccagtaatga tgacaacaca gatatctgtg tactgccaat tgagcgcttt
     1381 accaactaac cccacgaaag tagctagtgc gggaataatt accagtgcga accattttag
     1441 tacttcgaac gttttattat tcattttctt ctctccctaa acaaagtttt gatttgttgc
     1501 gtgtgttcca ccaatttttc tgtatgtgta tctaatcttt catcgtgttt ctttagttct
     1561 tcatgaatca tcaatcgatc tgatttgctc gattctaaat ctttagtcag caaatctaaa
     1621 ttgtggctta cttttgaaag agtctcagta attttcgaga aagatgcagc aatcggtcta
     1681 attactaata aaatcaaaga aacgatagcg gttattgatc ctgctatcat tccccattcc
     1741 cctaaattaa tcatgtgaca actcctttac cttaaataaa aacgcatcaa ttaagatgcg
     1801 ctcttatctt tattaatgat tttatctgct tcttcgtctg taatgcataa tggaacgaaa
     1861 accattactt gttcgttagt gaaacagccc cagtcataca ttagtttgat atcttcatat
     1921 gaatacatgt tacttcccac cttgtaattg ttttttgatt tcttcgattt ccttggtatt
     1981 ttgtacagaa gcaagcatcg tttttgaatt caactgcgct aaactttctg acttttcttt
     2041 caataagaca ttttcttgct tgattgctac attgtttagc attgctttag aatttagctg
     2101 tttcaattca tcattttctg ccttcagcgt ttcgtataat gccttaatat tagccaattc
     2161 atctacgcta tcaacagcat tcccttt
//
LOCUS       DAZKOR010000179         2187 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000179 DAZKOR010000000
VERSION     DAZKOR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.185"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            515..1474
                     /locus_tag="ACG8ZM_002836"
     CDS             515..1474
                     /locus_tag="ACG8ZM_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0493725.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1461..2066)
                     /locus_tag="ACG8ZM_002837"
     CDS             complement(1461..2066)
                     /locus_tag="ACG8ZM_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493726.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
BASE COUNT          744 a          358 c          327 g          758 t
ORIGIN      
        1 tttagataaa atagaaaaat atgaatatat tttaaatatt aagatgagag tcattaactt
       61 atgatagtag tcaatttgct tttcactaat tacaaaatat gtgatatagg aagtttagtt
      121 attgttctca taatgccatc ctgtttttag attatctagt aatttttttg ttatttaaaa
      181 ttaggagcag tgccaattgt tttaatttta aaggcttttt ttatatcttc taaattagaa
      241 gatatttgta tatcaatatg gaatatatta ccttttttgt atttttttat gttatcttta
      301 gctaaaacat caaatacatg tccttgaaat tgtatcgagg cagctggatt tagtttaaat
      361 tgaggaaact ttaccacagc tgattcaaaa cacttgtcac atttttttat ttttttacct
      421 tcgccgattg taaaattaag ctagacaact aaaaaagtca tttgtgctac actcaaaata
      481 gttccgtcga aagaattata aggagtgagt acaaatgacc tatacccatc ttacaccaac
      541 tgaactcgta atgatagaag cttatttcaa tcaatcacac tctgtttcaa aagtagccca
      601 tctggtacaa cgttctcgac aaaccattta taatgtctat cgttttctta aatctggtgg
      661 aagcgcaata agctactatg aacaatataa gaaaaacaaa cgtaactgcg gaagacgtcc
      721 gattgtgtta ccagatgggc aacaagaata cattcaaaaa aaggtggctc aagggtggac
      781 tccagatgtc cttattggtc gagcagagtt tcctattgct tgttcggtac gtacactata
      841 tcggatgttt aaagataaaa cctttgaccc tcacgattta ccaatgaaag ggaaaagaaa
      901 accaaacgga cataaagaaa aaagaggaaa acaagctttt cgccgttcga ttcaggatag
      961 aacaactgat tatatacagt tcaatcatga gtttggtcac ctagaaggtg acaccattgt
     1021 cggtggaaaa cataaaagtg cggtgatcac gttagttgag cgattatcta aagtgatcat
     1081 caccttgaaa cccgctggta ggcaagctat agatattgag aatagactga atcaatggtt
     1141 tcaatctgta ccgaaacact tgtttaaatc catcacattt gattgtggaa aagagttctc
     1201 gaattggaaa aagattagta ataccaatga tatttcgatt tatttcgctg atccaggaac
     1261 accttctcaa cgaggactaa atgaacattc gaatggctta ttacgtaaag atggcttata
     1321 taaagctatg gattttaata aggtatccga aacctttatc caatcggtgg catcaaaacg
     1381 aaatcatatt cctagaaaat cattacacta tcgcacacca ttggaagtat ttttgagtta
     1441 cgtaggtaaa gatgatttgt ctagcttaat ttgacaaacg aaactttgta aaaatattcg
     1501 attgaatatt ctatttggca cgactcactt ggcagtatat gtatgtcaat atttttcatc
     1561 tgtttcatag gtatttcaaa atctaaacta ttaaaaacat ataattccct gtaattattt
     1621 acatctaact ctactaaatt tatctttgga acaatagatc ctccacccat tccaccataa
     1681 taaaaaaaat cttgttctct atcaaaatag cacatattaa gctcagtttg tttatcatcc
     1741 tcaataattt tataaatcaa acctatataa tcatgaatgc tggcaaaaat tttttttaag
     1801 tcaagttcta aatcttcttc gaaaacacat ttaatttctc ctactgataa ttgatactct
     1861 atattacatg acttaattag ttttttagat cccccctcca gataggcata tgtttcaata
     1921 attatgttct ttttattgct tggcaaattt ccgttattta ttgcataaat taaaaatgac
     1981 tgtctattta gcccataatc taatacaact tcttcatact catatttctt taaatattta
     2041 atatgaaaat atgctgaatt aatcaaaact cgagtgttac tgttgttatt tattgttgtt
     2101 ctattgtgta taaaattgta taggttgtca tattttcttg gtttagtgaa ctactcacca
     2161 cttagctaaa cctaacggtt tcttacg
//
LOCUS       DAZKOR010000180         2125 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000180 DAZKOR010000000
VERSION     DAZKOR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.186"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..159
                     /locus_tag="ACG8ZM_002838"
     CDS             <1..159
                     /locus_tag="ACG8ZM_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0493727.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="ACG8ZM_002839"
     CDS             242..859
                     /locus_tag="ACG8ZM_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493728.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1023..1355
                     /locus_tag="ACG8ZM_002840"
     CDS             1023..1355
                     /locus_tag="ACG8ZM_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493729.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1679..1840
                     /locus_tag="ACG8ZM_002841"
     CDS             1679..1840
                     /locus_tag="ACG8ZM_002841"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFQ0493730.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            1872..2125
                     /locus_tag="ACG8ZM_002842"
                     /pseudo
     CDS             1872..2125
                     /locus_tag="ACG8ZM_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          758 a          272 c          429 g          666 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
      901 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
      961 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1021 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1081 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1141 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1201 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1261 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1321 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1381 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1441 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1501 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1561 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1621 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1681 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1741 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     1801 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     1861 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     1921 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     1981 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtgcaaga gtacagcaaa
     2041 gtcctcgatt atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac
     2101 gaaacctata tcaaaattaa gggac
//
LOCUS       DAZKOR010000181         2065 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000181 DAZKOR010000000
VERSION     DAZKOR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.187"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(129..416)
                     /locus_tag="ACG8ZM_002843"
     CDS             complement(129..416)
                     /locus_tag="ACG8ZM_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0493731.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(587..1939)
                     /locus_tag="ACG8ZM_002844"
     CDS             complement(587..1939)
                     /locus_tag="ACG8ZM_002844"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0493732.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT          563 a          412 c          357 g          733 t
ORIGIN      
        1 aaaaaggggt tgacaaagct tgtcataacc cctttttctg attaaaagat agtaaagaga
       61 cagtcatcgg taattgtgat gattttttgt catcacttaa ggatgagcgg ccttttcaaa
      121 aactgtcttt aaagatcatc atcagtttct agcacatatg gctgaccttc atatgtgtac
      181 tctgtattca atgtgccgtc attttcatct gagtagatgc gtctactgaa aggaaaacga
      241 tgagaaacat tatctttggc gtcatttgct ggcatcgtgt aatattcctc ttgcgtttca
      301 ttcaactgag agagtacttg atctgccaac tcatcaatat tgatcgttaa tttcttcgaa
      361 gcacttttat aatcttctaa aaatgcatct tgttggacaa aatattcttt attcatcttc
      421 tttctcacct ttcttttcaa aatcaaataa agctgcttgt tcttttatgt aacagcttta
      481 tctttgctgc ttattgtgac aaaaaattcg aagaaagaca actaattgta cttttcacaa
      541 aaaaagacag agggtcgtga caaaattgtg tcacaaccct ttttctttat tgtgcagttt
      601 cgatttcgcc tagcgctgct tgtgcaagta ggttcaagta attccatggg cggtcaaaat
      661 gtggttggaa gaagaaatct gaaagtgcga gatcttctac agtcatttta ttttgaacag
      721 ctaaagacat ggtatttgct gattgtgtaa tgtcgtattt ggacatgaac tgagcaccta
      781 cgatacgatt cgttcctttt tcatagacta gctccatcat tactttttca gtagttggca
      841 tgaactctgg acgataatta tcttcaaata cagtcgcttg gacttctaga tcgttcaaag
      901 ttgcgctttc tttggtcaca cctgtcgaac cgatcgtcca gccaaatagg taaagtcctg
      961 aagttccttg agtaccacga tacgccattt tatgatctgt caagttattt cccacaagca
     1021 aaccttgacg aacggcattt gttgccaaag gaatatagtt ttgcgtgcca cttggattgt
     1081 agttgactac tgcagagtct ccagctgcaa aaatgtctgg attacttgtc tgcatgtatt
     1141 cattcacttt gattgccccg tttggcagtg tatctacttt tccttcgatc agttttgtat
     1201 ttggacggaa gcccacacat aaaatgacca tatctgtatc aaatgtatct gttggtgtag
     1261 ctacttgttt cacttttcct tcttcatctg tgataaattc agtaacattt tcgcctagcg
     1321 caagagtaac accgcggtct gtcaattctt tttctaagat atctgtgaat ggtttatcta
     1381 gatatttatt caagatgcga tctaatccat caatcaatgt cacttgtttt ccagattcga
     1441 caaaagcttc tactaattca ataccgatat atccaccacc aacaattact acacgttttg
     1501 cttctttcgc ttgtgcaata atttcatttg cttgattgta gtttttgcat aggagtacat
     1561 ttttcgcatc gattccacta atcggtggga taataggcca tgaaccagtt gtcatcacta
     1621 atttatcata agaaaccgtt tctgtttctc ctgttttcag atcttttgcc gttactgttt
     1681 tattttctgt atcgacgtct gttacatcgt gttccatttt aactgttgct cctaatgaag
     1741 ataattcttc aggatttgag tagaacaagc ctgctgggtc ctttactact ccgccgacat
     1801 aaagtgcgat accgcaagat aagaaagaaa tattgtcatt tctttcatat actgtcactt
     1861 ctgcttctgg atgatttttt aaaatacttt ttacagctgc agtacctgca tgagtacagc
     1921 caattactac tactttcatt tatggtccct ccgattaaca ttttgtcctt cgacaaaact
     1981 actataacag aaataaaacg gatacaacag tttaatgctt gtgaattcac aaaggtttgt
     2041 ttgtagtttc attccttttc aagca
//
LOCUS       DAZKOR010000182         2063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000182 DAZKOR010000000
VERSION     DAZKOR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.188"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..789
                     /locus_tag="ACG8ZM_002845"
     CDS             52..789
                     /locus_tag="ACG8ZM_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0493733.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            817..1356
                     /locus_tag="ACG8ZM_002846"
     CDS             817..1356
                     /locus_tag="ACG8ZM_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0493734.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            1372..1542
                     /locus_tag="ACG8ZM_002847"
     CDS             1372..1542
                     /locus_tag="ACG8ZM_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493735.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            complement(1854..>2063)
                     /locus_tag="ACG8ZM_002848"
     CDS             complement(1854..>2063)
                     /locus_tag="ACG8ZM_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0493736.1"
                     /translation="IFVTILGLFELIQMNFFSFTHGIFTWFALSGLIVIDILNYFYIY
                     YSYLKNEKFFYTMFFPLVILIILLS"
BASE COUNT          867 a          285 c          310 g          601 t
ORIGIN      
        1 ttttacatcc tcttatgtct atgataacat aatttattga ggtgattttt attgagtaac
       61 gatatagttc gatataataa tggattaaat tctgtccctt taagaaaatt tacccctata
      121 gatatggatt tattttgggg aatatgttct aaaatgaaaa gaaaaggtac aaaagaagaa
      181 atttttacat ttaaacaaat aaaagaatta attgattaca atataaatgc acgtggcgac
      241 cgttttgcta atgatttaat aaaaatgtca aaaaaaatca ttactttgtc ttttatttat
      301 gaagacgaaa aaacatatga acaattaaat ctgtttcaaa aatttaaaat cgataaagtg
      361 aatgaaacat tattaataaa ggtttctgaa gaatttgaat atatacttaa ttcaatcgga
      421 actaatttta ctcgtttcga attagaaaat atgacaaact taaagagtag ctacgttaaa
      481 gagttatatc gccaacttat ggctcataaa gacagaagat ctaaaaaagg tcactggttt
      541 gtaaaaatag aagattttag agctctatta gcaataccag aaagttatcg catgagagat
      601 attgataaac aaatttttgc ccaagcgaaa aaagaatttt taaacgttaa ttcaactcac
      661 cctgctattt ttaaaacttt tgaaatcaaa aaaattaaag cctacaaagg aaataagata
      721 ggatctctgc aaatttattt tgaagaaaat ataccttcaa ttaccttaga taattggtta
      781 gagaaataat acctaacaat ttaggaggca attatcatgc aacccaaaga aacctttaca
      841 ataaaggaat tatctgaatt atttgaaata agtaggcaag caatctctaa acatgttgat
      901 aaattagacc cgtccttatt agctaaaaat gagcgtggtt ataaggttgt attacgttct
      961 ggagtattgc aacttgctag caacttaggt aaccaaaggt tactatatag gttgcatgag
     1021 tatgaacaag aaaacgataa aaacgtttat atatcaagca aaaacgtgat tgtaaacgag
     1081 ttgctagata agttgcgtga agaaaatgaa tttttacgag aacaactttc cctcaaagag
     1141 caaaataatt ctaatatgca aaaattactc gaccagcaac aacagctcac cctacaggca
     1201 aatcaacaga tcgaaaaact gcaagaacag ttacaattag cttatacgga agaaactcca
     1261 gaagatgaat caactactat tctttctgaa gaaacagaat cgatagaaaa acacacaaaa
     1321 aagaagaagc aaaaatggtg gcaactttgg aaataactgg agggaacatt catgacaaga
     1381 aaaaatcttc tattcggttc tattttacta ggaattctag gcttcagtcc gatgttattg
     1441 tcagtaatca ataaagggaa taaacatggt gaattgaaat tgaatccaaa ttattacaac
     1501 agtcctaacg aagaattgtt tgtaaaagag acggataaat aataaaaaag actgctcttc
     1561 cgagcagcca gttaaaaaat aatgagtgtt tattcttgaa aaagcacgcg cttatgaagc
     1621 gctaccaaaa atttaccata ttttacaaaa aataacaagt cttaaaaaaa tgacagataa
     1681 aaatgcacaa agaattagat atagataacc gcgaaaaatc aagctttaga aagttgcttt
     1741 tagctagacc aattaaaaat cacaaagtga gcgtctctat ccattggtag aggggctact
     1801 tggcatagtg tacattatgt caactatgca attaaataac taactacttc atattagctt
     1861 aataaaataa tcaaaataac tagggggaaa aacattgtat aaaaaaattt ttcatttttt
     1921 aaataagaat agtaaatata aaaataattt aagatatcta ttactattaa tccagataga
     1981 gcaaaccaag taaatattcc atgtgtaaag ctaaaaaaat tcatttgaat caattcaaat
     2041 aaacctaata tagtaacaaa tat
//
LOCUS       DAZKOR010000183         2056 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000183 DAZKOR010000000
VERSION     DAZKOR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.189"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            493..714
                     /locus_tag="ACG8ZM_002849"
     CDS             493..714
                     /locus_tag="ACG8ZM_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493737.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="ACG8ZM_002850"
     CDS             715..972
                     /locus_tag="ACG8ZM_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493738.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="ACG8ZM_002851"
     CDS             complement(1108..1710)
                     /locus_tag="ACG8ZM_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0493739.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="ACG8ZM_002852"
     CDS             complement(1777..1932)
                     /locus_tag="ACG8ZM_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFQ0493740.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DAZKOR010000184         1946 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000184 DAZKOR010000000
VERSION     DAZKOR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.190"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..546
                     /locus_tag="ACG8ZM_002853"
     CDS             64..546
                     /locus_tag="ACG8ZM_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493741.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            743..1633
                     /locus_tag="ACG8ZM_002854"
     CDS             743..1633
                     /locus_tag="ACG8ZM_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0493742.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          635 a          330 c          406 g          575 t
ORIGIN      
        1 taattttttt agatagaaaa ttgtatagta tgatgagaga aaaagtagtt tgggggaacg
       61 ttaatgagaa gaaaagcagt aatcgatcgg ctttatttat ttttttggtt ttttcctatc
      121 gttttgctac cttttacatg ggtaaatgct gttgtacaag gtgcggagat attaggcctg
      181 atccaaggaa gcggatttgt ggtttctagg aattttccag ctttgattgt tttctattta
      241 gtgataagtg taggtctctt attaccaaaa tctctgatca atcactacaa ttggcagctg
      301 ctttactttt cttgcttatt agtatttaca tctacgtttt ctttgctgcc tcgcttgatt
      361 atcggcccta atggtttgag cgagagtgga ttgaaggatt tagcttattc ctccatgtat
      421 ttcttgacga tcaaaccatg gttctattta gtaattgcct catttattct tgcaacattt
      481 ttttacttct ggacagaaat caggataaaa agtgaacgat ctgtatctat aaaaaataac
      541 tattgatggt ggatcatcag taaaaaaaca tatgagctgc gtaaaatgca tgtttctcat
      601 atgctttttt tattattatg taaagaatgc gaaaaacgtt tacatgattt ttacataact
      661 taactatatt tttacagcaa ccatacgagg gatttacagt cgactgataa actttagttg
      721 taaataagaa aggagcaata aaatgaaaaa agtattgttt actctaggta tgatgggaat
      781 tcttttagcc ggatgcggct caggatcagg aaatacagat ggctctgcca cacaaaataa
      841 ttcaagtgac tcaaatgaac aagtaaaaat cgttgcggta ggttcgacag cattacagcc
      901 gttagtcgat gcagcacaag aaagctttgt acaagaaaat ccaaattatc aaatctctgt
      961 acaaggagga ggaagcggaa ctggtttgag ccaagtcgaa gctggagcag ttactatcgg
     1021 aaactctgat gtatttgcag aagaaagaga cggagttgat gcttcaaaat tagtggatca
     1081 taaagtagca gtggtcggga tggcaccaat cgtcaataaa gatacagatg tcaaagatat
     1141 caccaaacag gaattgattg atatctttac aggaaaaatc actaactgga aggaagttgg
     1201 cgggaaagat caaaaaatca acgtcgtgaa ccgtgcaaac ggcagcggta cccgcgcgac
     1261 attcgaaaaa tggggactgg atggtgctac ccctgtgcag tcccaagaac aagattcatc
     1321 aggaacagtt cggcagctgg taagtcaaac accaggagca atcagctatt tagctttctc
     1381 atatctcgat gattccacac aagcactaag tatcgacggt gtagaaccaa aagaagagaa
     1441 tgtagcagat aacagctggg gaatctggtc atatgaacat atgtacacga acggaaaacc
     1501 ttcccctgaa gtccaaaaat tcttagacta catgatgaca gaagaaatcc aagaaggacc
     1561 tgtcaaagaa ttgggctatt taccaatcac aatgatggaa gtagaacgtg atcacgaagg
     1621 aaatatcaaa taactagcag ataaacatgc cacggtagtc gttactatta aggaaaaatc
     1681 aactatgttt ccccacatat acatttttcc tttaattgtg aaaaatagtt cctcatattt
     1741 tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttat tcgaaaaaaa
     1801 gagctacgac atagaactgt cgtaactttt tttggctctt cgtcaaatcg tgttggtgaa
     1861 gacaaatttg aataaaactt agaccgatat gcggcgcgtt ctgtgcgccg catatttttt
     1921 tagttttcaa agatcagacc ttgttg
//
LOCUS       DAZKOR010000185         1854 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000185 DAZKOR010000000
VERSION     DAZKOR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.191"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8ZM_002855"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACG8ZM_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFQ0493743.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACG8ZM_002856"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACG8ZM_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0493744.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          551 a          329 c          299 g          675 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     1801 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcga
//
LOCUS       DAZKOR010000186         1842 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000186 DAZKOR010000000
VERSION     DAZKOR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.192"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            582..1817
                     /locus_tag="ACG8ZM_002857"
     CDS             582..1817
                     /locus_tag="ACG8ZM_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493745.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
BASE COUNT          633 a          183 c          316 g          710 t
ORIGIN      
        1 acaaaaaaat attataaaga ataatatttt ctaaaaagta aactatagta atgtggatta
       61 tcaccttcca acagtatata aaatgagcgt aaaaacggct atttttgcct taacaacgat
      121 ttttttgtat acaaatacga tatgctcatt aattattgga atttgtagtg ctgtttaaat
      181 taatgaaggt gtaattatat gagtgatcat tttgaaaaag tttgttgaga agatgtgctt
      241 ctagtcatgt gatgggagaa aataatttaa ctgttagagt gaatagttaa attattctgg
      301 ttatatgaaa aaagtagttt ctaaaataat cttgcaggga ttaacttaaa gaaattgaga
      361 gaatagtttc tttgaaattt ctatatacaa tgagaagctg aaatttactt ttatttgatg
      421 actggaaata gaaattaaat atattgtatt gttacattaa agaaaacttg tatggaaaaa
      481 tcagtacaat accattattt aatgttcttc acttaaagcg caaaaaatat cagtttatta
      541 aactcgtata tagaacaaat atatgtagac ggggcgaaac gatgagtgta aaaaaaatgc
      601 tatttatggt aagtacaata attgtattaa catcccgatt ttggggaatt aatactagtt
      661 tatctatgcg atattttatt atggcaatat gggatttata ttttatgtta agctattttt
      721 cttatcgtga gaaaacatat aaaaaatacg ttatcaagaa taacttttgg ttgactataa
      781 tgccgtttgt gatattttct atctatactc taataatatg gggaattggt tcaaatgcag
      841 aatttgaaaa ttttataaag ttatgttcca cgttgcttta tttaattctt gcatatggtt
      901 acgcatgtac agcattttac ttatttaagg cagaaggaat tgattttatt ttttggtcag
      961 gtgttatttc atatatgagt ggttcagttt tttatttaat tgtatcttat ggtttaaatg
     1021 gtttaatttc ttatataaaa agtcttataa caggtgaaac aaatactgca aattttgcta
     1081 tggaggtaca tgatttaact tttgcgatgg gggttttttt cctttattac gtattttttg
     1141 aaaataaaaa tacaaaaaaa cataagataa aaattatcgt atctttaatt ttaattttca
     1201 tgggattgaa gagaattgaa ctgttagctt taagtggtgc aattctagtg tattatgttt
     1261 tattaaagtg gggaaaaaag attcaaatcc gtgcattagt gtgtactatt tgtgcaactg
     1321 ttatttcgtt agtgtttatt tacattatag atacaggaat tttggcattg cttatgtcaa
     1381 atttgggcat tacagatgac ggtgcaaggc tttcatttta tctatattca tctaaatttt
     1441 atgaacttgg tttaggatat gtaggaacag gatggacatg gttttcaaaa tactttttta
     1501 atctttattt atcgcacttt aggttaaatg gtggaagaat tgcagcaagc ctacacagtg
     1561 atatattaac tatttatata gaagttggat gtatagtttt tattatatgg atgttttggt
     1621 tattttttgt gaaaccgata agactaacta aaaaatttgg attgaaaaca ggggagtgtg
     1681 tattattatt aactatatat atgtttatat tatatttgac ggataatacg ctcacttatc
     1741 cagacactca aatgattttt ctcttagtac ctatgatagt ttcgtgggtt gataatcaac
     1801 aacgaatatc ggaatagata gctaaagtat attttaatta tg
//
LOCUS       DAZKOR010000187         1737 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000187 DAZKOR010000000
VERSION     DAZKOR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.194"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1025
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8ZM_002858"
                     /pseudo
     CDS             1..1025
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8ZM_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1009..1401
                     /locus_tag="ACG8ZM_002859"
     CDS             1009..1401
                     /locus_tag="ACG8ZM_002859"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0493746.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            1402..>1737
                     /locus_tag="ACG8ZM_002860"
     CDS             1402..>1737
                     /locus_tag="ACG8ZM_002860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0493747.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGI"
BASE COUNT          716 a          133 c          296 g          592 t
ORIGIN      
        1 caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct aatgcagtta
       61 ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa tctggtttta
      121 gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa gattgttatt
      181 taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa tatttaattg
      241 agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt ggttatgata
      301 gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt actaataaga
      361 aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat ttagaaacta
      421 atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct atactaggtt
      481 ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg tcagaagaag
      541 aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac ggtttagatt
      601 atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa gagtatatat
      661 tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat atagaaagtt
      721 ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta tgccataatg
      781 attttagttg taatcatcta ttgttagatg gcaataatag attaactgga ataattgatt
      841 ttggagattc tggaattata gatgaatatt gtgattttat atacttactt gaagatagtg
      901 aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga aatatagata
      961 ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt gaaactattg
     1021 tttattgatg ataatatggt ttttgatatt gataatttaa aaggatttct taatgatacc
     1081 agttcatttg ggtttatagc taaagaaaat aataaaatta taggatttgc atattgctat
     1141 acacttttaa gacctgatgg aaaaacaatg ttttatttac actcaatagg aatgttacct
     1201 aactatcaag acaaaggtta tggttcaaaa ttattatctt ttattaagga atattctaaa
     1261 gagattggtt gttctgaaat gtttttaata actgataaag gtaatcctag agcttgccat
     1321 gtatatgaaa aattaggtgg taaaaatgat tataaagatg aaatagtata tgtatatgat
     1381 tatgaaaaag gtgataaata aatgaatata gttgaaaatg aaatatgtat aagaacttta
     1441 atagatgatg attttccttt gatgttaaaa tggttaactg atgaaagagt attagaattt
     1501 tatgatggta gagataaaaa atatacatta gaatcattaa aaaaacatta tacagagcct
     1561 tgggaagatg aagtttttag agtaattatt gaatataaca atgttcctat tggatatgga
     1621 caaatatata aaatgtatga tgagttatat actgattatc attatccaaa aactgatgag
     1681 atagtctatg gtatggatca atttatagga gagccaaatt attggagtaa aggaatt
//
LOCUS       DAZKOR010000188         1735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000188 DAZKOR010000000
VERSION     DAZKOR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.195"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(474..773)
                     /locus_tag="ACG8ZM_002861"
     CDS             complement(474..773)
                     /locus_tag="ACG8ZM_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0493748.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(779..1009)
                     /locus_tag="ACG8ZM_002862"
     CDS             complement(779..1009)
                     /locus_tag="ACG8ZM_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493749.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(1268..1381)
                     /locus_tag="ACG8ZM_002863"
                     /pseudo
     CDS             complement(1268..1381)
                     /locus_tag="ACG8ZM_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          604 a          270 c          260 g          601 t
ORIGIN      
        1 aagagtatta tacaagctaa agcttttctt cattgccact caaatgagtg gcttttttat
       61 gtatcctttt atggattaat gaaaggatgt ttcacatagt tatatttctg tatatttgaa
      121 aagttttact ttgattttta aatagaaaga catttgggtt atattgtgag ataataataa
      181 agaagagttt aaagcgttcc ccaaaaacca ctgccccata agtgtgttac gctttaaact
      241 cttttatatt tgaagccatt aaaaagcata ccatattttt gaaaaaaagt gagaaaaaag
      301 gcttataatt ggagtggtag ttaattagtg acttattttt gattttatag cactgatact
      361 ataaaatata gatatcatca tattacacaa tcttaatact aacttaaaaa atatctcctt
      421 tcataagtat ggtgataaaa tccgttccgg gctacctttt taggtagcct actttaatct
      481 ttgtatcttt ctggatcaac gaaagtatac tttatatagt cataacgccg atgatcgctt
      541 cgagcgtcta gcacatcagt cacgatatca aacaaaaaat atacgtcttt cttcattcta
      601 gttttcgcag cagggatttt aaagtagttc ttattagaat agtagagatt aattaataag
      661 ctatcttcga ttgctaaaaa gaaaacttct gaatcccaca ccttataaaa atctttgaca
      721 aatctattcg aaggatcaaa tttaaaccat aattgcgtct cattaaaaag cataaccatt
      781 actccgatct atttttaaac ttagtctcta cctctaatat atatcgagtt tttattttgc
      841 cttcagagaa tactgtttct ttttttgcag ttacaggttg tttattttcg gaaaaagcta
      901 atatagctaa aattgaaaca tccatctgga atttgtcttt ttcgttgctt tgctcataaa
      961 aatctgcata ttcatcacta atattttttc taataaattc ttccatcata aagatcacct
     1021 cgaaaagagt atacgaacaa acgttctttt tgtaaaggtg gaatttatta tactaaataa
     1081 ataggtgaac aagtacttgt gccaaattat gtgccaaaaa aaatcgaatt taataaaaca
     1141 caaacaaaaa gaaatcctat tatgataaca tttcttataa taacaaacac aacagaagac
     1201 gtgtaatagt tagtcaggaa cgtacaaata acccctgtat cctttgcggt acagggggtt
     1261 attttgtata agtagacatt gtagtttcta aactgctatg tcctaaacgt ttcgatacgc
     1321 taagtatatt tactccttga taaagtaaaa tagatgcatg cgtatgtctt agtccatcaa
     1381 taaacagtcg aagagaaaat agggaaaaat tgattactaa agaaaaactt actaggtcga
     1441 cggtgtagtt gattttcata cttcaataga ttcgaattaa acgatttcga ttggcttata
     1501 ccagtcctat ccaacgaaag atatatcgtt cttaactcta gcggtataac ttgaagcttt
     1561 acgtaaagcc aatgggaatg aactggagat gactttttat tatttttagt aatagctgat
     1621 gggttaatac ttacaacttt ccttttcaaa gtaccatata aaaaaacggt ggggttgttt
     1681 agcgaaataa aggatctaag ccaatagctt agatccttta ttctatgctt tatat
//
LOCUS       DAZKOR010000189         1683 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000189 DAZKOR010000000
VERSION     DAZKOR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.196"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..542
                     /locus_tag="ACG8ZM_002864"
     CDS             144..542
                     /locus_tag="ACG8ZM_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0493750.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1220
                     /locus_tag="ACG8ZM_002865"
                     /pseudo
     CDS             526..1220
                     /locus_tag="ACG8ZM_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
BASE COUNT          641 a          244 c          324 g          474 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcagcgataa
     1081 cgttaggtaa agagctacta gatcaagtat tagagatgcc ctcgattgat aaagcagtga
     1141 ggggcttcca agaagattgg gtgcaaactt tccaagcaga aacgattggt gaactaaaaa
     1201 aggtcgctga ttacttctga atcaatttct ccataaaata aacgatagcc ttctcgaaaa
     1261 ctcgaggagg ctatctgacg attcaatatt cattaattta aagtttgggt gactattact
     1321 aactattact tatttctcat ttaaacgatg tattccctaa cctctaatac atccttgtaa
     1381 ctctgaagtt acgccagttt tgaataaatt tcataaaaac aaatgaaaag tgtcgcataa
     1441 aaatatcttt cgtgtttctc tagtataaca gaataagaag aacaagtcca gaagggatat
     1501 ttttagtctt ttttaaaata acttttcagt cgagtttgag tttatgatag ttgtttttga
     1561 agcgatacgg caagtgaaaa aatagggaag catgaaagaa tctacttaat ggcgcattga
     1621 ttcatgtgat agaaataaaa aactttttta aattatatcc tcatgattgt aacaaatcca
     1681 gtg
//
LOCUS       DAZKOR010000190         1664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000190 DAZKOR010000000
VERSION     DAZKOR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.197"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8ZM_002866"
     CDS             131..505
                     /locus_tag="ACG8ZM_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0493751.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8ZM_002867"
     CDS             complement(619..1248)
                     /locus_tag="ACG8ZM_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0493752.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8ZM_002868"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8ZM_002868"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZKOR010000191         1647 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000191 DAZKOR010000000
VERSION     DAZKOR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.198"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG8ZM_002869"
     tRNA            complement(20..91)
                     /locus_tag="ACG8ZM_002869"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG8ZM_002870"
     tRNA            complement(113..200)
                     /locus_tag="ACG8ZM_002870"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG8ZM_002871"
     tRNA            complement(225..298)
                     /locus_tag="ACG8ZM_002871"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG8ZM_002872"
     tRNA            complement(312..382)
                     /locus_tag="ACG8ZM_002872"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG8ZM_002873"
     tRNA            complement(384..459)
                     /locus_tag="ACG8ZM_002873"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACG8ZM_002874"
     tRNA            complement(478..551)
                     /locus_tag="ACG8ZM_002874"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACG8ZM_002875"
     tRNA            complement(564..653)
                     /locus_tag="ACG8ZM_002875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACG8ZM_002876"
     tRNA            complement(677..750)
                     /locus_tag="ACG8ZM_002876"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACG8ZM_002877"
     tRNA            complement(764..837)
                     /locus_tag="ACG8ZM_002877"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACG8ZM_002878"
     tRNA            complement(865..937)
                     /locus_tag="ACG8ZM_002878"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACG8ZM_002879"
     tRNA            complement(957..1030)
                     /locus_tag="ACG8ZM_002879"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="ACG8ZM_002880"
     tRNA            complement(1040..1113)
                     /locus_tag="ACG8ZM_002880"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="ACG8ZM_002881"
     tRNA            complement(1124..1209)
                     /locus_tag="ACG8ZM_002881"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="ACG8ZM_002882"
     tRNA            complement(1226..1297)
                     /locus_tag="ACG8ZM_002882"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="ACG8ZM_002883"
     tRNA            complement(1309..1381)
                     /locus_tag="ACG8ZM_002883"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="ACG8ZM_002884"
     tRNA            complement(1399..1480)
                     /locus_tag="ACG8ZM_002884"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="ACG8ZM_002885"
     tRNA            complement(1488..1560)
                     /locus_tag="ACG8ZM_002885"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="ACG8ZM_002886"
     tRNA            complement(1564..1636)
                     /locus_tag="ACG8ZM_002886"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
BASE COUNT          438 a          460 c          391 g          358 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataactt
//
LOCUS       DAZKOR010000192         1594 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000192 DAZKOR010000000
VERSION     DAZKOR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.199"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..>1594
                     /locus_tag="ACG8ZM_002887"
     CDS             275..>1594
                     /locus_tag="ACG8ZM_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493753.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRI"
BASE COUNT          577 a          223 c          290 g          504 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatc
//
LOCUS       DAZKOR010000193         1570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000193 DAZKOR010000000
VERSION     DAZKOR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.200"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1518)
                     /locus_tag="ACG8ZM_002888"
     CDS             complement(52..1518)
                     /locus_tag="ACG8ZM_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0493754.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          514 a          331 c          291 g          434 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa attttcagaa aatattatac
     1561 atctttaccg
//
LOCUS       DAZKOR010000194         1534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000194 DAZKOR010000000
VERSION     DAZKOR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.201"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..1252
                     /locus_tag="ACG8ZM_002889"
     CDS             83..1252
                     /locus_tag="ACG8ZM_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0493755.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          547 a          250 c          253 g          484 t
ORIGIN      
        1 aaatagaaat ttagggtaga aaaaaaagtc cattctgtaa aattaaagtt accacacaga
       61 aattttatag gaggactttc tcatgactaa atttactata gaaattatgc aaacactact
      121 caataaaggg gatttagatg aattatttca ggatcattta gagcgagcga ttaattcact
      181 tctacaagca gagctaactg cttttttaga ttacgaaaaa tatgatcgaa atggattcaa
      241 ttcaggaaat tcccgtaacg ggaattattc acgtacattt aaaacagaat atggcgagtt
      301 gaatttaact attcctagag atcggaatgg cgaattttct caacaaacac taccagcata
      361 taagcgaacc aatgattcat tagaaacgac cattatccaa ttatttcaaa aaggcattac
      421 tatggctgaa atctccaaat taattgaaaa aatgtatggt caccactata cgccacaaac
      481 aatctccaac atgagtaaat tggtggctga agatgtttta gcttttaaag aaagaacgtt
      541 agaagccaat tattccgtta tatttatgga tgcgacgcat atccctgtga agcgacagac
      601 tgtttcgaag gaagcagtgt atattacgat tggtattcgt ttggatggaa ccaaagaagt
      661 tcttgggttt actattgccc caactgagtc tgcttatatt tggaaagaag ttcttcaaga
      721 tcttagaaaa cgtgggttag aagaagtttt attagtagtg acagacggat taagctgtat
      781 tgaagaaagt atccatagtg tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt
      841 atctagaaat atcgctcata aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt
      901 caaattggtt taccaagcga attcaaaaga agaggcattg gatcacatcg actttatgac
      961 taggaaatgg aaaaagcagt atccaagagt cgtcaattta ctcttgaatc ctgccctatt
     1021 aaccttttat aatttccctc acgccatcag acgaacaatt tattcgacga acctgattga
     1081 aggctttaat aagcagctaa aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga
     1141 atctctagag agattcttca tttctcaatt taatcaatat aaccaaaaat ttttaggtag
     1201 aattcacaag ggatttaaag aaattcagga tacattagag tcgatgattt aactgtaatt
     1261 aacagaatgg attttccatt tacatataat tcttgacgct accataatct aaataaccat
     1321 catattcatc tactacttat ataattgagt tttattaaac tttttaagtt tctattctat
     1381 caaatattta aacaatctct gcatccgaaa agactacctc gtatagtttt actaacaata
     1441 tttttattag cgtgttcaaa tcctgatttc ttgtatcaat tattttctgt tcactttcaa
     1501 taagaaaaaa gtctttactt ccatggataa taat
//
LOCUS       DAZKOR010000195         1532 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000195 DAZKOR010000000
VERSION     DAZKOR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.202"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1342
                     /locus_tag="ACG8ZM_002890"
     rRNA            <1..1342
                     /locus_tag="ACG8ZM_002890"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1437..>1532
                     /gene="rrf"
                     /locus_tag="ACG8ZM_002891"
     rRNA            1437..>1532
                     /gene="rrf"
                     /locus_tag="ACG8ZM_002891"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          428 a          329 c          450 g          325 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 acttcaaatc cagttttgag tgaacaaaat ttactcaata aaagataaca ccacagtgtg
     1441 gtggcgatag cgagaaggat acacctgtaa ccatgccgaa cacagaagtt aagcttctta
     1501 gcgccgattg tagtgagggg ttgccccttg tg
//
LOCUS       DAZKOR010000196         1526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000196 DAZKOR010000000
VERSION     DAZKOR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.203"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..780
                     /locus_tag="ACG8ZM_002892"
     CDS             97..780
                     /locus_tag="ACG8ZM_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0493756.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
     gene            complement(817..1314)
                     /locus_tag="ACG8ZM_002893"
     CDS             complement(817..1314)
                     /locus_tag="ACG8ZM_002893"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0493757.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(1370..>1526)
                     /locus_tag="ACG8ZM_002894"
     CDS             complement(1370..>1526)
                     /locus_tag="ACG8ZM_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0493758.1"
                     /translation="SSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGT
                     LRYSLGY"
BASE COUNT          482 a          273 c          254 g          517 t
ORIGIN      
        1 gtctaaacat gttggtaaat caacaaaaaa acgcatatag tagtctcaaa actcccaaga
       61 aatgaggact atttaacaaa ctacgatggt tacgtcgtga caactgaaca ttttgactta
      121 gccaatcctg ttactaaagt cgatgatatc cccgattatg aaatgtacag tcaaacaatc
      181 gatagcttaa ataagcgttt tggcaatcgt gttttaaaag gcatcaaaat cggttacatc
      241 gcttctgaaa aggatcggat catcgactat ttagccgata aagattacga tttaaaatta
      301 ctcagtgtgc atcataatgg gcaatttgat tatttagatg acgaagtcaa agatatggat
      361 cctgctattg tgattcctca gtattttgca caactttcag aagcactagt tgttatagaa
      421 gcagatgtct ttgctcattt tgactatggt tttcgggtat ttggtctatc cgttgctgaa
      481 tttaaacaat atgaagcgca gtttttaccg attctagatc aagtaatcaa aaataaattg
      541 gcattcgaat taaatgctaa gagcgcttac ttgtatgaca atttagcgtt atatgaatat
      601 gtcatcgatt tatatctttc acgtggtggt accttgttta gtgttggatc agatgggcat
      661 taccttgaac atttccgttt ccatttcgat gatctttttg ctctgttaaa agcaaatggt
      721 gtgacggaat tagcgattta tcaaaaagga aagcgaatta tggttccatt accagtctaa
      781 aaaaacgtcc tcagcatcaa aagtgaggac gtttttttag atttcttttt ctaaaatcgg
      841 acaaatacga tcttgttttc ctaaaggaaa atgtttgatc ccgatttttt taaatccatg
      901 acgttcataa aaacgaatac ctgcatgatt tttttcataa acagtcaaac gtaaataacg
      961 tttttgtttc gttttagcat aacgattcgc ttcatcgagt aaccgttgcc ctaacccttt
     1021 gttttgataa tctttgagca gatagagccg ttgaatctct atagcatctt tttcatcatt
     1081 gactttcata taaccaatga gtatttgatg atccttaata ccaataaatt tactggttga
     1141 ctgtgccaat tctactgcca atatttcagt tgtcaattgt gtctgtaaat atccttgcat
     1201 atcaagtgga tctgcgccat cgataaacga ttcataaaaa ctgacaacac ttacaacttg
     1261 taaagcatct agatcatcat aatctaaata cgctagtgtg atttgattgt tcatctaact
     1321 cctccttttg acatagtata ctataaatca cccgtggtgc tagttaattt taatatccta
     1381 aagaatatct caaagttcct tcaaaacgtc gttgtgctcg acttaataga acactgtaag
     1441 ccagtaatat gctttcaaat ctactctcta gccctttaac agaacgtgag ttgaaatttt
     1501 gacaacctaa tttttctaag gaagaa
//
LOCUS       DAZKOR010000197         1471 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000197 DAZKOR010000000
VERSION     DAZKOR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.204"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..496
                     /locus_tag="ACG8ZM_002895"
     CDS             86..496
                     /locus_tag="ACG8ZM_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0493759.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            510..866
                     /locus_tag="ACG8ZM_002896"
     CDS             510..866
                     /locus_tag="ACG8ZM_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0493760.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          540 a          210 c          271 g          450 t
ORIGIN      
        1 ttttttttat attttattag ctaataattg gtattttaaa agaaattact gtagaattgg
       61 attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta gctgtacttc
      121 ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag agaagaatat
      181 ttttgccgaa ccgttaacag aaagtgagat caaaaagatc ttggcagcga ctgaaggagg
      241 agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca tagattttaa
      301 cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat tattgagacg
      361 acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg agatccggtc
      421 atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac aaaacaataa
      481 catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga tcagcgcact
      541 gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct tatcaatttg
      601 ttgacttaga agaacgcgat ttaagtacag aagaagcaga agaattatgc agttttgaag
      661 aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac attgcgattg
      721 acctttcctg tgtaggaaaa aaacaactga tttttttatg cacacaacat accaatctcc
      781 tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat gagcagttga
      841 tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca ctgagcattt
      901 catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct ttttttgact
      961 ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa tctaggtggg
     1021 gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc atttctgttg
     1081 ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag ttgttgacat
     1141 gttagaaatg acatgataag ataactgagt tgttaaaaaa gaaccacttg tgaagcttga
     1201 ttctatcgaa aaaaacttca aaaaagttct tgacaaataa caaatgataa gttatgataa
     1261 atgagttgct gtaagatttc gaaaaacgaa atcaaaaaaa cttttaaaaa agagttgaca
     1321 attacttgtc tctctgatat gatataaaag ttgctacggc agccaaaaac gaatcgggga
     1381 acactcctga tgaagtagac ctttgaaaac tgaacaaagt aagacaaacc aaatgtgtag
     1441 ggcgtcttga ttcaattcaa gacaacaaac a
//
LOCUS       DAZKOR010000198         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000198 DAZKOR010000000
VERSION     DAZKOR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.205"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8ZM_002897"
     CDS             complement(150..1337)
                     /locus_tag="ACG8ZM_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0493761.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKOR010000199         1408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000199 DAZKOR010000000
VERSION     DAZKOR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.206"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            360..902
                     /locus_tag="ACG8ZM_002898"
     CDS             360..902
                     /locus_tag="ACG8ZM_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493762.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1058..>1408
                     /locus_tag="ACG8ZM_002899"
     CDS             1058..>1408
                     /locus_tag="ACG8ZM_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0493763.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPD"
BASE COUNT          551 a          171 c          270 g          416 t
ORIGIN      
        1 ctgcgcgagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt gacatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcaa attgatgaag cagatcccaa
      181 agggctgctt catcaatttt ttcgtaagtg tccgtacact tactgtgctt gctcatatat
      241 taatcgatca tatactaatc gtgtaagaac aactttaagc ataatactta tattgttata
      301 ccatagtttg gaaaaataaa aaattatatt tcatcaacgt ttcataaaag gtgtgtagaa
      361 tgttattgaa agtagctata aaatgctatt gtgtaaaaaa taaaggagga ataatttatg
      421 aaaaaatagt accaagagga gcatgcctat tacttgcatt atgtttgagc ttaagcgcag
      481 ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga aggacaatcc ataagttctg
      541 aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga aacttttaag ccatcagatt
      601 atacattgga ggcaaagaaa gaatatgttt atgaatattt agggcttaaa tttaaactct
      661 cagacaaatt tagaaattat atagctgata aaaagatagc gatgttagat gatcaaagtc
      721 ctatagataa agaactaaag tatgctattt tgacatttga gaaaatgaca gaagaacaaa
      781 agaatgcagt tatagaaaag atgggcgatg gatataaaaa ttggcaaaat gagcttgaaa
      841 gaattgggac tattggtata tttgaaaaaa atacatcaga agaaaaaaat ctaaaattat
      901 aaaatgtgat actcatacta aaataggagt ttcaagtgat ggaaaatacg attgctattt
      961 aagcacaaat agcggagctg aaagtaatct tctggatgaa ttaaaaagaa ctgagattca
     1021 aattatagat aaaaaagaac gtcctaaaaa tggttttgtg ctatcagaaa aaactgattt
     1081 agaaaataca gaagcattta acaaagaatc ggttaaagat ttacgtaaac taccaacaaa
     1141 ggacataaat ggcaaggatt ttacaagtaa agattttgag aagtatgatc ttactatggt
     1201 aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa attcctgatt tggttgaggt
     1261 tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga gttgttacag atcctgttga
     1321 tgataacggt gaaaacaagg aagcaattga aaagtcaaag ttaatacatg aaaagacaaa
     1381 ggcttcatat cctttcttaa tgccagat
//
LOCUS       DAZKOR010000200         1384 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000200 DAZKOR010000000
VERSION     DAZKOR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.207"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..752
                     /locus_tag="ACG8ZM_002900"
     CDS             <1..752
                     /locus_tag="ACG8ZM_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0493764.1"
                     /translation="EQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALSRHVDLEDKM
                     GSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIRKHGGYLTPE
                     KVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSSTDSVTISQI
                     AADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPKADGGTKIVM
                     NTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            767..970
                     /locus_tag="ACG8ZM_002901"
     CDS             767..970
                     /locus_tag="ACG8ZM_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493765.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            986..1324
                     /locus_tag="ACG8ZM_002902"
     CDS             986..1324
                     /locus_tag="ACG8ZM_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0493766.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT          534 a          214 c          273 g          363 t
ORIGIN      
        1 tcgaacaaaa cgaagttcgg acaattttag taaatgatga accatatttt gtaggcaaag
       61 acgttgcgag tgttttgggt tattcaaaca ctaaagatgc tttgtcgcgg catgtagatt
      121 tggaagataa gatggggtcg cgaatcacga cctcaggtca atcaagagag atgacaatca
      181 tcaacgaatc tggtttgtac agtttaatct taaaatcaaa acttccctct gccaaaaaat
      241 ttaaacgttg ggtaacaagt gaagtgttgc cagcaattag aaaacatgga ggttatctaa
      301 ctccagaaaa agtagaagaa gctttgctta atccagatac aatcattcaa ttagcaactc
      361 aactaaaaga agaaagaact ggaagattaa tcgcagaaca aaagattgca gagtacgaac
      421 ctaaaatctc ctatttagat agcatattat cttctacaga ttcagtaaca attagtcaga
      481 ttgcagcaga ttatgggatg tctccacaac agatgaataa attacttcat aaactaggta
      541 ttcagaaaaa agtcggtaac caatggttat tgtgcaaaaa acacatgaac caaggataca
      601 caaaatctca tacaactgag atcccgaaag ccgatggtgg cactaaaatt gtaatgaata
      661 ccaaatggac acagaaaggg cgtctattta tctacgaatt actaaaaaaa gaaggatatt
      721 accctcaaat ggatttagag gaaattggtt agaaaggagt tttagtatga ctgacattgc
      781 agaaatcact caacgagata gagaaaaaat caaagaatat gtcgaaagtt caaagttctt
      841 aacttacacc atgcttgctg aaagatttgg aattagcaaa agctacttat ctttaatttt
      901 aaacggtaaa aagacttctg cagaagcaaa cagaattata gattcgatta tcactatgta
      961 cgaattgtaa agggaggaac agctaatgca atatctagaa gcaaaaatcc caattccaga
     1021 aggctatgta attatctccc aagtggatta tgaggagtta aaaaaagctg atgatactgg
     1081 tagatggatg acgttgccag aagtgctaga acggattaac agaaaatatg attggtttac
     1141 ttctagagtt ttaaagaacc caagatatag aaatattatc gatatcgaga aaaacaaaaa
     1201 tggatttgtc tattatccag ttgaaggaag agacacatat ctatttttaa gaagtaaaac
     1261 acttgaattt ttagaaacaa atttttcgga aatcttaagg aggcaagcgg atgggaaaat
     1321 ttaatagagc gctagtgttc agcgcaccgc taatcatcta cgctttagga ctttggggaa
     1381 gcag
//
LOCUS       DAZKOR010000201         1349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000201 DAZKOR010000000
VERSION     DAZKOR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.208"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..505)
                     /locus_tag="ACG8ZM_002903"
     CDS             complement(<1..505)
                     /locus_tag="ACG8ZM_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493767.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILIS"
     gene            471..>1349
                     /locus_tag="ACG8ZM_002904"
     CDS             471..>1349
                     /locus_tag="ACG8ZM_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493768.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIK"
BASE COUNT          441 a          195 c          188 g          525 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaa
//
LOCUS       DAZKOR010000202         1349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000202 DAZKOR010000000
VERSION     DAZKOR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.209"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..>1349
                     /locus_tag="ACG8ZM_002905"
     CDS             56..>1349
                     /locus_tag="ACG8ZM_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0493769.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          421 a          270 c          257 g          401 t
ORIGIN      
        1 acaccctgaa atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc
       61 tcatactcag cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga
      121 aaattgttta actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct
      181 tctgtacaag ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg
      241 ttgggggacg acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct
      301 ggagctgaag aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac
      361 ctctgttgct gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg
      421 cttaaaagag aatacttcca tgacggaaat cgctcgtcag aaaaatattt ccacttcttc
      481 ggtctatcgt gtcatgaagc gattctaccg cccattgaat cctttcaaac agactttgcc
      541 aaaggtctta tgctttgatg aattcaaatc ggttcgaagt gttgccagtg caatgagttt
      601 tatcatgatg gacggacaaa gccatgcatt attggatatt gtcgaaaatc gacgcttgcc
      661 ttatttagaa cgctattttt ctcggttttc attagcgact cgtgaagccg ttcaattgat
      721 tgttattgat atgtatgcgc cttatgtttc gttggtgaaa aagcttttcc cgaatgctca
      781 gttgatcatc gatcgtttcc acatcgtcca acatattggc agaactttct tgaatcatcg
      841 agtgaaagaa accacggtac gacttaaagg aaattcccac tctcaacgag ggttagggaa
      901 aaaattgaag cgctattgga aactcctaca aaaagaagaa gcaaagttaa actatgaaaa
      961 acgggtttgg cgtgccagtt ttaaagatta tttgactgaa agtgagattg ccgatcgact
     1021 gttagctgct tgtcccaact tacgccaagg ctatcagtta taccaagaca ttctttatgc
     1081 ggtaaagaaa agagatcagt cactatttga ggattgttta actagagaca ttagcggtct
     1141 gccagaaacc tatacaacca ctttacgaac gtttaaaaag tttctgccac agatccaaaa
     1201 tgcgcttcac tacagctatt caaacggacc attggaatgc ttaaacaacc acatcaaagt
     1261 attaaaacgc aatgcgtacg gctttcgcaa cttttacaat ttcaaactac gaattatgat
     1321 tcgtcatggc aaaacatttt taaccaaat
//
LOCUS       DAZKOR010000203         1328 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000203 DAZKOR010000000
VERSION     DAZKOR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.210"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1328
                     /locus_tag="ACG8ZM_002906"
     CDS             82..>1328
                     /locus_tag="ACG8ZM_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0493770.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFE"
BASE COUNT          451 a          258 c          253 g          366 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaa
//
LOCUS       DAZKOR010000204         1254 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000204 DAZKOR010000000
VERSION     DAZKOR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.212"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..741
                     /locus_tag="ACG8ZM_002907"
     CDS             70..741
                     /locus_tag="ACG8ZM_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0493771.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(901..1092)
                     /locus_tag="ACG8ZM_002908"
                     /pseudo
     CDS             complement(901..1092)
                     /locus_tag="ACG8ZM_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          460 a          189 c          175 g          430 t
ORIGIN      
        1 aaatttgata ttatatggca tgaacagaat taaaatatca gttaaaataa aagttaagga
       61 gcattgtgta tgacaattaa aactagtctc caaaatcaag cttaccaatc tattcgccga
      121 caaattatct atgctgactt agaacctgga aaaaaagtat cagaaaaaaa attagaagaa
      181 acactttcta ttggtcgtac accaatacgt gaagcactga ttcaactccg acaacaaggt
      241 cttgttgata caattcctca aagcggaaat tatatctcat taattgattt agattcggcc
      301 aaaaatgctc gatttgtcag agaacatttg gaacgacaaa ttatgttaga gtgttgtgcc
      361 aaaatgaata accaaaaaaa acggatttta gaaactataa tttcagaaca agaaaaagct
      421 gttagtcaaa aaaatgctcg tagttttttt cataatgata atttattcca taaagcttgt
      481 tttgaaattg cagagcgaga agaagtatgg caatggttag aaaataacaa tacacactta
      541 gaacgatttc gatggctacg agtaaaaacg gaaggattaa agtgggaaaa aattatggaa
      601 caacattatc aactatttga ggcattaaca aaacaagatc cagaagaagc taactttcta
      661 acatcgttgc atcttcattt aatgcttaat gagcaaaata ttgttttagc tactcatcca
      721 aaatatttca aaaatatata acttttaact ctttttaaat taaatggttg ttctgatcag
      781 aaaaatatcg cttgttttct tattatgtaa tatatttcat aaactattct gcataacaac
      841 tattgtttgt actttttaca ttggataact aattatgact gctgataact tttgatatta
      901 aactagtatc tccaatacaa ataaaaaata gatatcttgt tcatcaaaat tagttcgtgg
      961 atacttacct tcatttacat cattcagcgg tttcccattt tcttttattc tattatttca
     1021 ttttctaata gacttggctt catttcgcaa tacttttttt caataggcat caaattggtg
     1081 ttgtttagat atttagttgt agttttgtga catgataagt atgctccttt ttctcttttc
     1141 cactaataca acgatcaaag ggtgcttttt tcaaaaaact agaaaaatca aaataagatt
     1201 cgtattttga ttctatatat cctgtttcaa ctacatctta tatttcacaa taga
//
LOCUS       DAZKOR010000205         1253 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000205 DAZKOR010000000
VERSION     DAZKOR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.213"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..906
                     /gene="scrK"
                     /locus_tag="ACG8ZM_002909"
     CDS             34..906
                     /gene="scrK"
                     /locus_tag="ACG8ZM_002909"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0493772.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          376 a          230 c          302 g          345 t
ORIGIN      
        1 tgatttcatt ctaaaataaa gggagaaatg tctatgctat acggtgcaat tgaagcaggc
       61 ggcacaaaat ttgtgtgtgc cattggcgat gaagaaatga cgatcaagga aagagtgagc
      121 tttccgacaa cgacacctga ggaaacaatg cctttagtca ttgacttctt caaacaatac
      181 caagcagatt tagcagggat tgggattggc tcatttgggc ctatcgatat ccatcgcgat
      241 tccgccactt atgggtacat tacctcgacc ccgaaattgg catggcaaaa ttttgatttc
      301 atcggtacca tgaaaaaaga atttccgatc ccgatttctt ggacaacaga tgtcaatgcg
      361 gctgcctatg gtgagtatgt cttcggcagt ggcaaaggat tgagcagtgt cgtttactat
      421 acgatcggta cgggtgttgg cggcggtgcc ttacaagaag ggcgctttat cgaagggttt
      481 agtcatccag aaatggggca tatgctcgtc gtccctcatc caaaagatga ctttgctggc
      541 agctgtccat ttcatggcaa ctgtttagaa gggatggccg ctggacccgc catcgaaaac
      601 cgcttaggga aaaaaggaca agaggttgca gaagatgatc cttattggga aatcgaagcc
      661 tcctatattg ctcaatgtgc ctacaataca accttgatgt tttctccaga tgtgatcatt
      721 tttggcggtg gtgtgatgaa acaacgccat atgctgcaaa acgtccacga cgcatttgca
      781 aaattggtca atgggtatgt cgaaacacct gagttaagca aatatatcgt gaccccagca
      841 ttagaagaca acgctggaac gctcggttgt ttggcattgg ctcgagaagc ggtcttacat
      901 tcataaacga aaaaaggctc ttcgacattt agtgttggta gagaaagata caactgaaaa
      961 tttagtcata accagttagg cagagtgctt aactggtttt tgttttactt ggaagatcaa
     1021 gccttgaatg agataaattc ttgctcgaaa gttatggaaa tttcgatagc cgtaggccac
     1081 ttgtattgtg caatagtttt ttacaaaaaa aagggggtgc ggatattttt ttggaccttt
     1141 ttaacaaata ggttctgttg caaagtttta aatctactat cagataaggt agaataatag
     1201 aaaaagatag caggaggaat gacgatgagt cattttaaag gaaagcaatt tca
//
LOCUS       DAZKOR010000206         1215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000206 DAZKOR010000000
VERSION     DAZKOR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.215"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG8ZM_002910"
     CDS             414..947
                     /locus_tag="ACG8ZM_002910"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0493773.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAZKOR010000207         1195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000207 DAZKOR010000000
VERSION     DAZKOR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.216"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8ZM_002911"
     CDS             <1..1076
                     /locus_tag="ACG8ZM_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0493774.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZKOR010000208         1141 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000208 DAZKOR010000000
VERSION     DAZKOR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.218"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..328)
                     /locus_tag="ACG8ZM_002912"
     CDS             complement(<1..328)
                     /locus_tag="ACG8ZM_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493775.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVY"
     gene            complement(330..>1141)
                     /locus_tag="ACG8ZM_002913"
     CDS             complement(330..>1141)
                     /locus_tag="ACG8ZM_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0493776.1"
                     /translation="RVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFR
                     KSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKT
                     PLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKV
                     DLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNY
                     AFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          442 a          191 c          138 g          370 t
ORIGIN      
        1 tgtaaacatt ggagaaaagt atttttgtga aataattttc cccctgtata aaaatttcat
       61 cataaacgga taaatcatcc gagaaaaatt gattatggtt agttaacaag ttaattttac
      121 gtttaaagga agaaccgatt ttccggtgat tcacaaaaac aacatcatta aatagcccta
      181 attcttttat acgctcacta tatcttctag cgttttcaaa ttcagaataa ataaataaat
      241 ctccttttga ttctcggtaa aaattttgat aaacatttaa tatattaatc aaatgtaaag
      301 gagtccaagc aatgaaagct gtcttcatat taattctcct tttttaaata caagtaagaa
      361 atgcttccca aataatacca gaaaacaatt gaaatgacat cgcgcttatt gaaaagtaca
      421 tgagcctcca ccatttcatc tataaaaata gatagtataa aaattaaaac aataccaaga
      481 aacgcataat tcactttact tctaaaaaat gttttgagta aactaaagat atttaacaca
      541 tagaaagcat agatacatat aaaagcaatt ataccacttg atacaagtac ttgaagaaat
      601 ccattatgca tgtttccatg tgctcttttt agttcattca catctgtagt atcaagatct
      661 acttttttca aaaattttgc atcgctattc ccatagaaat cggtctctcc ataaccaaat
      721 aacaaagttg gtcttataac tttaagcgca gctttccaaa tcgtaaaacg accagctgaa
      781 atttcggagt tatcactaga atgctgtaat attacttctt tttctggaag tggagtttta
      841 tctgattcat ttgactctat aacttctact tttctatcaa tcgatacagg aattgttgct
      901 aaaaaatcct tagcataatt agtaccaaaa gaaaaatata ccaatagaac taataatatg
      961 cttaccatcc atttgatttt tacaggtagt acttctctaa tgctctttct aaattttgaa
     1021 ttggcaaaca acaaaagaaa taatactaaa aaagaacaaa ttactacctg ggcacctcta
     1081 gacgaagaaa gtgtaatata aaatatttgt acgataaaat tcatcatata aaaaacacga
     1141 a
//
LOCUS       DAZKOR010000209         1136 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000209 DAZKOR010000000
VERSION     DAZKOR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.219"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1061)
                     /locus_tag="ACG8ZM_002914"
     CDS             complement(<1..1061)
                     /locus_tag="ACG8ZM_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0493777.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITE"
BASE COUNT          301 a          254 c          182 g          399 t
ORIGIN      
        1 tcttcggtta ttctttcttc ccaggatttt ggtcttaagg cctcattcag attgataaac
       61 agctttttta ctcgtttctg atactccttc atgacctttt tcattaatgt tttgattcta
      121 cgactataga ttccatagcg acgaatcgtt ttgaaattct tattttgaat atggcgaatc
      181 agtgcagcta tgaattcgtc tatactcatg gtcatcgtct ctttagtatt cgttcgtttg
      241 tccatgtagt tgaacatgac agtgtcgcca tcatacatta agattcggct taatgcgatc
      301 gggccacgct tcatataacg actgatgtat tggagtagtg actttagatt tgtcctgctc
      361 cttttaggag catttacgta aaacccttct ccattgttct tgtacgcagc ttgtaatctt
      421 gattgaactc tttgcttgtc ccactcacct aacgttcgtc taattagttt caatacagca
      481 tttttccagt attttcttaa catagaaaaa ggtaaataat cgtattcttc ccaacttcca
      541 tctcttgtta agccacccat agtaacaacc atatgaacat gcggattaaa ttctagtttt
      601 gatccaaagg tgtggattgt agctactata cctgccttca cctttctttt ctcaaaatag
      661 gcaagaataa tttttgcggc ttcatccatt agtcctttca gtaaggaatc tcgatagttt
      721 tccatcataa agatttcccg taacccctca tcgatcgtaa agattacgtg ccgatgtgtc
      781 actttaaaca tatcattcgc cacaaccgct gaccaccttt ggctttcacc tgttgcacac
      841 gttggacaga actttccacg acatttcaag ggaacaaact ttgtttgatg acatccctcg
      901 cagacaaaca gtcggtatcc tttttgaata tctccacaat atctaaactt gtttacttcc
      961 cgtttgacga ctagccgaat ctttcgcccg tatttctcaa caaagttatc ccaattatgg
     1021 tgttcgtcaa aaaagattgc ttcgagaata ttcgctttca tagtaatatt ttacttgttc
     1081 aaaaacaaaa tgaaaagggc taaaagactg gtagtgcagc ctttttagcc cttaac
//
LOCUS       DAZKOR010000210         1105 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000210 DAZKOR010000000
VERSION     DAZKOR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.220"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACG8ZM_002915"
     CDS             <1..1087
                     /locus_tag="ACG8ZM_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0493778.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DAZKOR010000211         1059 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000211 DAZKOR010000000
VERSION     DAZKOR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.222"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..993)
                     /locus_tag="ACG8ZM_002916"
     CDS             complement(37..993)
                     /locus_tag="ACG8ZM_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493779.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgt
//
LOCUS       DAZKOR010000212         1045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000212 DAZKOR010000000
VERSION     DAZKOR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.223"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACG8ZM_002917"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACG8ZM_002917"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0493780.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DAZKOR010000213         1002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000213 DAZKOR010000000
VERSION     DAZKOR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.225"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..>1002
                     /locus_tag="ACG8ZM_002918"
     CDS             15..>1002
                     /locus_tag="ACG8ZM_002918"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0493781.1"
                     /translation="MILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLVTPFCFIS
                     KQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPRCLLFDEF
                     RSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHYIVTDMNA
                     PYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQYRQLKSL
                     FKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQDLLTAYQ
                     EKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLENLH"
BASE COUNT          301 a          209 c          190 g          302 t
ORIGIN      
        1 gcaagaagat ctctatgatt ctcctgaatc gtagcggtaa taaacggacc tatcttcggt
       61 tgaagaaaca gcgatacctc tgccgagcat gtaaaaaata ttttaccgct cggacgtatc
      121 tcgttacccc attctgcttt atttctaagc agattcatta caagatctta gaagaattga
      181 cagagcgtca atctataaaa gcaattggta aacattgcga tgtttctgtg acaactgttc
      241 agcggacatt gtccgccttg tcatcagata tccgtcagaa tcttgagtat ttacctcggt
      301 gtctattatt cgatgagttt cgttctcttt ctacatggca cggtaaattt tctttttctt
      361 gtatggacgg tgagaccggg aaactgtttg agatcttacc tagccgccgt aaaaaagatc
      421 tcgttgcttt tttcatgcgt ttttctctca aagcgaggtt gggtgtacat tacatcgtaa
      481 cggatatgaa cgctccttat ttctcactag ttaaagagtg ttttccaaat gcacaagtga
      541 tcgtcgatcg gtttcacatt gttcaacatc tgaaccgctt cttcgatgcg gttcgaaaac
      601 gtgtgatgaa aacacttgat caaaaagacc caatacaagc taaacagtat cgccaattga
      661 aatccttatt caaattactt ttaaaatcag aggatcgttt agattacaat acactaacga
      721 aaagacgcaa tttcaattgg agactattga ctgaaacaga agtagttgac cgccttctaa
      781 agatttctcc tgaacttaaa acagcttatc gttactacca agacctgctt actgcgtatc
      841 aggaaaaaga tccagatact tttttccagc tgcttcgagc aatgcctgga gaagttccat
      901 tggaactgca ccatatcaaa aaagcctttt tcaaatacga gaaagggatt cggctggcat
      961 tactgaagaa gtattcgaat gcacgactgg aaaatctcca ta
//
LOCUS       DAZKOR010000214         1001 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000214 DAZKOR010000000
VERSION     DAZKOR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.226"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1001
                     /locus_tag="ACG8ZM_002919"
                     /pseudo
     CDS             370..1001
                     /locus_tag="ACG8ZM_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          184 g          294 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DAZKOR010000215          998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000215 DAZKOR010000000
VERSION     DAZKOR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.227"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..634)
                     /locus_tag="ACG8ZM_002920"
     CDS             complement(80..634)
                     /locus_tag="ACG8ZM_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0493782.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          323 a          185 c          107 g          383 t
ORIGIN      
        1 ttccgaaaat tcagttatct tcattaatct caccttctaa gttttcatta tacacaaaaa
       61 atatatccta atatctcaat tagcctccca atttaaaacc acgtaacgat gcccctattt
      121 catcttcagt tataccaatg tacctttttg taattgcttg cgagctatga ttaaaaataa
      181 acattaaggt agcaatgtct ctagttttct tgtagtagtg atacccaaaa gtttttctta
      241 atgtatgtgt tcctatatca tctcttccta atttttctgc tacatctgca tatatctgat
      301 agacacgatc ccttgttata tgtcgacctt gttgccgact tggaaataac caatcatttt
      361 cttctttacc ttcagtatat aataaaatct cttctctcaa attatctata aaaatcggtt
      421 ttcgcttacc tgttttatgt tcaacaataa taggcttact tttacctcgt atgtctccaa
      481 cttttaatcc tataatatct gacatacgta acccattatt gattccaaac aaaaataaaa
      541 aactatctct ttctggatta cttgttcttc ttagccaaaa ctgaaaatct tgtatctctt
      601 catttgtcct taacggttgt acattatagc tcactattgt ttcccttctt aaaaatattc
      661 tgagttcatg attatatata tattttacca taatcatgaa cttatatata tactctgtta
      721 aaagtgaaga aaatcaaggg gtttagttta tgattttata tcatttcagt aactcagaat
      781 attccgactt ttttgtacaa aaaaattaac cttacttata tgaagattct aagctcttat
      841 ttaaatttta gacgggcagt tatccgtaga agttcttaga atagctaatt tacataatta
      901 aattatatga aatttaagtt tgaacaatat actaagaact ccccttacta taaagtgaaa
      961 taaattagca tatgaataac cgtgctacca aacttttc
//
LOCUS       DAZKOR010000216          959 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000216 DAZKOR010000000
VERSION     DAZKOR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.228"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACG8ZM_002921"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACG8ZM_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="ACG8ZM_002922"
     CDS             complement(570..>959)
                     /locus_tag="ACG8ZM_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0493783.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAZKOR010000217          957 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000217 DAZKOR010000000
VERSION     DAZKOR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.229"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          365 a          109 c          181 g          302 t
ORIGIN      
        1 tttcttggct acttttttat ttttatgtat ctacaaaagt ttccaggggc agaacctgtg
       61 attattaatg gaagaacctc aagtattgtc ttaaatcatt gttatgaaaa tggattaaaa
      121 gagttcatca aggtgtgatt aacaagttac ttttcattac atcgaatttt tcagaagttg
      181 cttatattgg tgatgatatt aatgatttat catgtatgac atctgtgaag aagcgggagg
      241 aatagtagaa tgtccaaaaa atgccacaag aaatgagatt gaattatctg attttattgt
      301 aactcacagt ggtggtgacg aatctgtgat agagtttatt gagtggatat tggaatcgat
      361 atagtgtagt agtacagaaa aattatcata caattaatac taggcataag aatattggca
      421 tattctagag ttaaaagtaa ctattcgaaa aggataaggt tagaattttt tgttttgcca
      481 gtggaagaat ataaaaaggg cattggaaat taatatatta gaatgctaag cggagaaaaa
      541 aatatatgaa aagtacgtcc ttgtgatggg atggaaaaat acacttattt caaacaacga
      601 taatataatt atgtgaaccg aagtatactc gcttctaatt caatcctcaa atcccgacag
      661 tcgcgacttc aaaaaagcgt atagttttaa tgtatcccca tatgatccga aaaatggtaa
      721 tttacaatta acagtttgat gatgttgaag atgtggaaat gctgaaatta ccatgatgag
      781 aaacacatta attaaaggga attaattaac tagaatataa tctgattcaa tttgaattaa
      841 gcaaaaaaag gctgagtttt aaataatcgc gaattgtaaa attaagttag aaaaataaaa
      901 aggcatttat ggtacactca aattgtattt ccgacgaaag aaaacaaagg agtgtaa
//
LOCUS       DAZKOR010000218          943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000218 DAZKOR010000000
VERSION     DAZKOR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.230"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG8ZM_002923"
     rRNA            <1..>943
                     /locus_tag="ACG8ZM_002923"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZKOR010000219          938 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000219 DAZKOR010000000
VERSION     DAZKOR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.231"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..406
                     /locus_tag="ACG8ZM_002924"
     CDS             89..406
                     /locus_tag="ACG8ZM_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0493784.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="ACG8ZM_002925"
     CDS             complement(497..862)
                     /locus_tag="ACG8ZM_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493785.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          349 a          116 c          156 g          317 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactt
//
LOCUS       DAZKOR010000220          924 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000220 DAZKOR010000000
VERSION     DAZKOR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.232"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..690
                     /locus_tag="ACG8ZM_002926"
     CDS             <1..690
                     /locus_tag="ACG8ZM_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0493786.1"
                     /translation="NPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSE
                     KAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
BASE COUNT          368 a          122 c          189 g          245 t
ORIGIN      
        1 aatcctgaag gacaaaaagt gactacagac ttaaataaag agccagacgc atctgaggga
       61 attaagaaca aagaagatct accaaaagga actacttata cttggaaaga gaaagtagat
      121 gttagtacag ctggaaacaa aaaagggatt gtagtagtaa catatccaga tggatctaaa
      181 gaagaagtag aggttactat ttctgtagaa gataaaaaag caccgaataa acctcaagtt
      241 gatcctatta cagagggtga ccaaattgtt actggtaaaa ctgaaccaaa tgcagaggtg
      301 acagttacat taccaaacgg gagtcaatac catgggacag ctgataaaaa tggtaatttt
      361 acagttaaag ttcctaaatt agaggcagga acaaaagtga tagtaactgc aactgatgaa
      421 tctggtaata ctagtgaacc tactaatgta gttgtttctt caaacgaaaa agacagtgaa
      481 aaagccgtta gtaaagataa taagactgac aatcaaggta gcaagcaaaa caaaaataga
      541 ggcaaaagca gtcctcaaaa gcagtcctca aaagcttatc ctaagacggg agagattgat
      601 agtaatatct ttactattag tggtggttta atcctgttag gaactttagg gttattaggg
      661 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaaatct tctctgaaaa
      721 gaggaagaat ctttttcttc ctctttttgt tttttatttt gggagatagg ttgttagtta
      781 aaaatgaaga tacttttcat agaagggaaa agaatgtgat taagtgaaaa aaatagtaat
      841 tattggatca aaccattcgg ggctagctgc agctcgattt ctaaacttat gtgattcaaa
      901 ttatgaagtt attctgatag ataa
//
LOCUS       DAZKOR010000221          904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000221 DAZKOR010000000
VERSION     DAZKOR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.233"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>904
                     /locus_tag="ACG8ZM_002927"
     CDS             <1..>904
                     /locus_tag="ACG8ZM_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0493787.1"
                     /translation="SNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLIL
                     SFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKK
                     QWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMS
                     TNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSG
                     LSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGII
                     AAGYTTVFTYFLLFIFHYNIAKKIT"
BASE COUNT          280 a          113 c          151 g          360 t
ORIGIN      
        1 tcaaatgctt tttttgatat gaaccaactt gtatttaatt tgatgttatt acaaagtttt
       61 gctagttttg taattagtta tatccagtct tattttacga ttaactttaa ccattttaaa
      121 tatttgattt tatcttttat atctactttt ttgaatattg caatctctgt agctttgatg
      181 cagacattat attttgatga taaatatgtt ggcagagtag tcggcggaac actgtcattt
      241 gtgttgatag gtttagtaat ttatttaaga ataatgataa agggtgctca tttttttgat
      301 aaaaaacagt ggagatacgc tttaaaaatc tctttgccgc taatcccaca ttccttagca
      361 aatatagttt tagcacaatt cgatagaatt atgattaatt cttatttagg tcctgctgcg
      421 gggggaatat attcctacat tagtaacata ggtattattc taagcgtaat atggatgtct
      481 actaataacg cctgggttcc atggttctat ggtgagatga gtaaaaataa tgaaaaaaaa
      541 gtgaagaaag ttagtaacta ttatttagta gtctttactt tgattacgat gattgtaatg
      601 gtagtttcga ttgatttagc aaaatttatg gctccgaaag actattattc tgggttatct
      661 ttagtggttc cgataacctt aggctatttc tttcagtttt tatatagttt accagttaat
      721 gctgaatttt atgagaaaaa aacatcgttt attgcgattg gtacagtagc aagtgctata
      781 gttaatatac tattaaacat gattttcata cctaggttag gaataattgc tgctggatac
      841 acaaccgtat ttacgtattt tttacttttt atttttcatt ataatatcgc taaaaaaatt
      901 acga
//
LOCUS       DAZKOR010000222          887 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000222 DAZKOR010000000
VERSION     DAZKOR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.235"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..476
                     /locus_tag="ACG8ZM_002928"
                     /pseudo
     CDS             1..476
                     /locus_tag="ACG8ZM_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
BASE COUNT          342 a          124 c          131 g          290 t
ORIGIN      
        1 ggtgatacga tcgttggcat tcatcataaa agtgccgtca ttactttagt tgaaagatta
       61 tctaaagtca ttatcacgat taaacccaac ggccgtaagg cattagatat tgaaactgcc
      121 cttaatcaat ggttttctcg cttccctaaa aacttcttta aatctattac gctattacgt
      181 ttgactgtgg aaaagaattt tctaactgga aagccattag taaccaacat gatattgata
      241 tatattttgc ggaccctgga acaccttctc aacgcccatt aaacgagaat tctaacggga
      301 ttctgcgtcg taatggactg ccgaaatcaa tggattttag agaagtgaat cagacattta
      361 tttccagtgt cagcaatcaa cgtaatcata ttccaagaaa atcattgaat tacagaacac
      421 caatcgaaat atttttgagc tatgtacaag aagcatttta ttctagctta atttgacaaa
      481 tcataaataa taaagaattg aacttctgag aaaacaaaga atcttttttc tttttttgaa
      541 ttgactcatg gattggcgtt aaaattttat tttgaaaaag ttatttctgg tttaactcaa
      601 aaaaagagat atgaatctaa tagtgataga aaggatgtta tttttcatga aatggttgtt
      661 ttagatttaa tacatataac taataaatgc tattgaatgt atttaaacta attaacaaaa
      721 gaatttgaat tgttaataaa atccaataaa atatattaat aaagtagata gaaataaaaa
      781 aacaaagact aaacatatat aattggatta aacgcaatat ctgcaaaata ttgtaaattt
      841 aaagcctttg tagaaaaatt tactgaaaga caaactgata agattat
//
LOCUS       DAZKOR010000223          873 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000223 DAZKOR010000000
VERSION     DAZKOR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.236"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACG8ZM_002929"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="ACG8ZM_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0493788.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          250 a          186 c          133 g          304 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaattat acagaaacac tttattcaaa ataggaaaga
      841 tcaggaaagc catcaattgg aggaaaaaac tct
//
LOCUS       DAZKOR010000224          870 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000224 DAZKOR010000000
VERSION     DAZKOR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.237"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..>870
                     /locus_tag="ACG8ZM_002930"
     CDS             2..>870
                     /locus_tag="ACG8ZM_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493789.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLI"
BASE COUNT          272 a          115 c          130 g          353 t
ORIGIN      
        1 catgaggtgg tatatgaatt tgaaaaaaat tttttctgta aaaatattta tagtttatgc
       61 atttgttagt ttattatatt tatttccagc atttaaaaac atggaaatat ttggctttta
      121 tgatacggca tttcacatta atcgtgcgtt aagtttagaa tctattttta gctcccctat
      181 taattttgaa acgtttagat catatggcat gcaagtaaat aatttttacc catggttaac
      241 tctatatcct ttatttttat tgatcaagtt tactaattta gcaataggct ataacctttt
      301 tctttatatt gttacgctca ttactctatt tatatgtcat tatgtaatgt atgaaattac
      361 caaaaaacat gttactagct cctttttcgc tattatatat acaacatctt cttttaggag
      421 tgtagaaata tttcttcgag gggcaatggg ggaattactc gcaatgtcaa tacttcctct
      481 tattttactc gggtttataa agctttacga ctcaaaaaaa gaatcttggg ttatgttggc
      541 gattagcatg actttattaa tttacactca tgttctttct gtagctatga ctatggtaat
      601 gattataatt gctttaatta ttcaatttta tcggaaaaag aaaatagaaa tcttcttaat
      661 tattaaatta ataaaagctg ctatagttac tttacttctt tcactagcgt ttataggtcc
      721 tatgattgag cagacattgt atcaagatct gaatagacct tatgtggata tattacaaaa
      781 aagagcaatt tatctgggta aagagttttt gattggaagt atagattcag aattactatc
      841 atttggtatt ggcttaactt tgcttatttg
//
LOCUS       DAZKOR010000225          852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000225 DAZKOR010000000
VERSION     DAZKOR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.238"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..307
                     /locus_tag="ACG8ZM_002931"
     CDS             134..307
                     /locus_tag="ACG8ZM_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493790.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          285 a          142 c          104 g          321 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tccacacctt catactctat
      841 cattttttat aa
//
LOCUS       DAZKOR010000226          816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000226 DAZKOR010000000
VERSION     DAZKOR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.240"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>816
                     /locus_tag="ACG8ZM_002932"
     CDS             <1..>816
                     /locus_tag="ACG8ZM_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0493791.1"
                     /translation="NVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQRINREDL
                     SDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAKQNNLKLG
                     DEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEIL
                     NNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGI
                     KYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFE"
BASE COUNT          348 a           66 c          140 g          262 t
ORIGIN      
        1 aatgttaatc aatttaaaga tattgaaaaa ttaaaagaaa ttgaagaaaa aataattcaa
       61 tatgatggat tagcaaaact aaaagatgct aaagtagtta gcggagaaca aagaataaat
      121 agagaagatt tatctgacga atttaagaat gttgtttcac tcgaagctac aaataatact
      181 aaaagaaata ttttatttag tagtggagta tttacaatta aagaaggtaa aaatatagaa
      241 gaaaatgata agaattcaat tattgttcat gaagaatttg ctaaacaaaa caatctaaaa
      301 ttaggtgatg aagttgatct tgaattacta gatattgaaa aaagtggaaa aataaaaagt
      361 cataaattta aaattatagg gatcttttct ggtaaaaaac aggaaacata tacaggatta
      421 tcttctgatt ttagtgaaaa tatggtgttt gtagattatt caacaagcca agagatatta
      481 aacaattcag aaaataatga aattgcaaat aaaattttaa tgtattctgg tagtgcagaa
      541 tctacagact tagccttaaa caaattgaaa gagcttaaaa ttgatgagtc aaagtatttt
      601 gttgaaaaag attctaatgc gtttgaagag tctttagagt cagtgagtgg aataaaatat
      661 ataataaaag taatgactta ttctattatg ttgggtggga tggttgttct ttcattaatc
      721 ttgattctat ggttaaggga aagaatttat gaaataggta tattcttatc tattggaaca
      781 tctaagatac aaattataat gcaatttata ttcgag
//
LOCUS       DAZKOR010000227          791 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000227 DAZKOR010000000
VERSION     DAZKOR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.241"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..716
                     /locus_tag="ACG8ZM_002933"
     CDS             <1..716
                     /locus_tag="ACG8ZM_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0493792.1"
                     /translation="KKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTST
                     ESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADK
                     SAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWS
                     ESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNE
                     TANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          199 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt t
//
LOCUS       DAZKOR010000228          772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000228 DAZKOR010000000
VERSION     DAZKOR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.242"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..539
                     /locus_tag="ACG8ZM_002934"
     CDS             <1..539
                     /locus_tag="ACG8ZM_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493793.1"
                     /translation="KIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDET
                     DIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISP
                     ISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKI
                     LLPENWAELRKLVEYGII"
     gene            526..>772
                     /locus_tag="ACG8ZM_002935"
     CDS             526..>772
                     /locus_tag="ACG8ZM_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0493794.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDI"
BASE COUNT          277 a          105 c          116 g          274 t
ORIGIN      
        1 ccaaaataga tgaaaacagt aaattgatga aaaacataca atcaatcttt gggatagaag
       61 aagtttttgt cggagataaa tgtatctttt ttgatcaacc tttattatct gatggttatg
      121 gcatagatga aacagatatt ttcgctaaag taaatagtat attttctgga aaaaaagaaa
      181 ctttaattaa gcttcatcct agagcaagga caaatcgtta tggtgaaaaa aacatattga
      241 attccagttt accgtttgaa gtcttgctac ttgaaaatga cttctcttca acacttttga
      301 ttagtcctat atctacaatt tctttttcac catctatgat gtttgaaaag acgatcaact
      361 ctattatgtt ggctaaatta attttaaatg aatatacagc attatctgaa gataaaaaag
      421 aaacattgac aaatattgtt cttttctgtg aacattataa ccgtctatca gaagaaaaaa
      481 ttttactccc agaaaactgg gcagaattaa gaaagttggt agagtatggg attatttaac
      541 acactttcca agtcaaaatt agttaagtcc agtttctggt atactatagg gaattttttt
      601 attaagggaa tttcattcat aactattcct atttttgtaa atattactac tcaagcagaa
      661 tatggtttga ttaataattt cacttcattt gtatcaattt tttcgattct tgttgggcta
      721 agcctaaatt cggcaattaa taatgcaagt tttgactata aagatgacat aa
//
LOCUS       DAZKOR010000229          674 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000229 DAZKOR010000000
VERSION     DAZKOR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.243"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            281..484
                     /locus_tag="ACG8ZM_002936"
     CDS             281..484
                     /locus_tag="ACG8ZM_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFQ0493795.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            484..>674
                     /locus_tag="ACG8ZM_002937"
     CDS             484..>674
                     /locus_tag="ACG8ZM_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0493796.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIV"
BASE COUNT          250 a           81 c          115 g          228 t
ORIGIN      
        1 cgcctttagt gactagagtt tgcacaattt ctgtagtaaa atcattcata gggaagtcct
       61 cttttctgtg aattggttgt cgttaacttt attctacaga agcgacttcc ttttttgtat
      121 ggattttttc atttacacaa aatattttac actctcgtca atcattgtta tataataaaa
      181 tgttatataa taaaattact aatgatattt aatattacaa gcaatcttaa gaaattcgaa
      241 aaggaaaaaa tgattatgaa aaagaaagaa aaagaaagta ttgaaacatt gtgttattct
      301 aggaatatta ggaacttgtc tagctggcat tggtacagga atagacgttg acgcagctac
      361 ttactatgga aatggtcttt attgtaacaa agaaaaatgt tgggtaaatt ggggacaatc
      421 atggtcagaa gggttaaaac gttggggaga taatcttttt ggtagtttta gtggaggacg
      481 ataatgactt caataaacat tgatcacaca aaaaaagtca tacatgaatt atacaatagt
      541 ttaatgacac gacctaatca gacgaaagca ttattagata ttactgatgt tttatttcag
      601 gtatataaaa aaattgatac agcaaaatat cctgaaattc ttgtaaataa attagttaat
      661 tatatttaca tcgt
//
LOCUS       DAZKOR010000230          649 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000230 DAZKOR010000000
VERSION     DAZKOR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.244"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>649
                     /locus_tag="ACG8ZM_002938"
     CDS             <1..>649
                     /locus_tag="ACG8ZM_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0493797.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DAZKOR010000231          642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000231 DAZKOR010000000
VERSION     DAZKOR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.245"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..>642
                     /locus_tag="ACG8ZM_002939"
     CDS             20..>642
                     /locus_tag="ACG8ZM_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0493798.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANK"
BASE COUNT          252 a           64 c          139 g          187 t
ORIGIN      
        1 attagaggta taaaaagtta tgaaacacaa ggtaagtgtg atagtaccaa tttacaatgt
       61 tgaaaaatat ttaagaaaat gtgtggatag tttattgagt cagacattaa aagagataga
      121 aatcgtatta gttgatgatg gttcgccaga tgcttcgggg gaaatagcag atgagtatca
      181 aaaaaaatat tctaatgtaa aaacaataca tagagaaaat ggggggttag gaccggcaag
      241 aaatacagga attgagaatg cgacaggaga atatgtggct tttcttgatt cagatgattg
      301 ggtagaaagt gatatgtatg aaaaattata cttagttgcg tccaaagaaa atgctgatat
      361 agtagttagt ggacattgtg attttgcgaa tgacaaagct atggtgaaaa agcaacatcc
      421 tttgagtggg aatgtatatg atacgcctaa tagtattaaa aatgtacgaa aaaatttatt
      481 tggacatagt ccaaaagatc aagaagtaga ggccttcccg atgtcagtat gtatgtcttt
      541 gtataataaa aatctgctag ataaatatag actaagattt gaaaaaattt tatctgaaga
      601 tacaattttt aacttgaatg catatgatgt cgcaaataaa at
//
LOCUS       DAZKOR010000232          621 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000232 DAZKOR010000000
VERSION     DAZKOR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.246"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          229 a           71 c          139 g          182 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc t
//
LOCUS       DAZKOR010000233          616 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000233 DAZKOR010000000
VERSION     DAZKOR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.247"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          236 a           80 c           80 g          220 t
ORIGIN      
        1 agcaatatat aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga
       61 gcaaatataa aatttaataa aaataagaac taaataggaa aacgtagacg taattttcta
      121 cgttttcctt ttcatatata ttaaatatta cggaaaatta tataaactaa tttagtgttt
      181 tgtttgtaat tcctaatttt gctccacttt tagataattt ttaggtctat ttaactcatt
      241 agacattcta tgctcatttt tctccaaatt tgtttcaact ctttcgtact taattttgcg
      301 tatttgaaaa ttcttctttt agagtacttt tattacgata ctagtatgat atatatctac
      361 agcaaacagt taaaatcttc ctaaaataag cgaatagcat tcctaaaacc tataaaattt
      421 ttagatgaag cgacaagtgg acaattaata aactacttga acatgaaatt atggatcacc
      481 tttcaaattt agaaaatgga attcttatac ttattgcgca acatttattg attgcaaaat
      541 tatgtgacaa aaagaactag tagaagaagg aataatggct tgataataaa gtactaccta
      601 ttaaaggtag tacttt
//
LOCUS       DAZKOR010000234          572 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000234 DAZKOR010000000
VERSION     DAZKOR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.248"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          176 a           92 c           62 g          242 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga at
//
LOCUS       DAZKOR010000235          551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000235 DAZKOR010000000
VERSION     DAZKOR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.249"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>551
                     /locus_tag="ACG8ZM_002940"
     CDS             <1..>551
                     /locus_tag="ACG8ZM_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0493799.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSN"
BASE COUNT          203 a           87 c           95 g          166 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc acgattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aattatcaaa
      361 aatcacgata tcatctgtat tgaagactta aacacaaaag gcatgttgcg taatcataaa
      421 ctagcaaaaa gtatttcgga tgtttcttgg tctagttttg tgtctaaatt acaatacaag
      481 gctgactggt acggtcggaa aatcatcaaa gtagataagt ggtttccatc tagccaaatc
      541 tgttcgaatt g
//
LOCUS       DAZKOR010000236          523 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000236 DAZKOR010000000
VERSION     DAZKOR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.250"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>523
                     /locus_tag="ACG8ZM_002941"
     CDS             <1..>523
                     /locus_tag="ACG8ZM_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0493800.1"
                     /translation="LTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNKFKQGK
                     TALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVSCGMRT
                     LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEG
                     DTIVGIHHKSAVIT"
BASE COUNT          185 a           93 c          102 g          143 t
ORIGIN      
        1 cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt
       61 gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt caataagttc
      121 aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt
      181 ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact
      241 cttggttgga cgcctgacgt cattatcggg cgaaaagaaa ggtctgtttc atgcggtatg
      301 agaacacttt atcgtttatt ttctaaagga atatttgata ttgacacact accgatgaaa
      361 ggtaaaagaa aacccaatgg ccatcaggaa aaacggggaa aacaacagta tcagcgctca
      421 atccatgatc gacctgataa ttatcctgat ttcaattctg agtttggtca ccttgaaggt
      481 gatacgatcg ttggcattca tcataaaagt gccgtcatta ctt
//
LOCUS       DAZKOR010000237          499 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000237 DAZKOR010000000
VERSION     DAZKOR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.251"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..>499
                     /locus_tag="ACG8ZM_002942"
     CDS             3..>499
                     /locus_tag="ACG8ZM_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0493801.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          181 a           90 c           82 g          146 t
ORIGIN      
        1 ctatgaatga ttttactaca gaaattgtgc aaactctagt cactaaaggc gatttaaatg
       61 aattattccg ttcgcactta gaaaaagcga taaacacact cctacggact gaattaacgg
      121 cttttttaga ttacgaaaaa tatgatcgca ctggttttaa ttcaggtaat tcgagaaacg
      181 gttcttactt tcgatcaatc aaaaccgaat atggtgaatt aacattggaa atacctagag
      241 atcgtaatgg tgagtttaaa caacaaactt taccagccta caaaagaaca aacgatacat
      301 tggaaaccac tattatccat ttattcgaaa aaggtgttac gatgtctgaa attgctgatt
      361 tgatcgaaaa aatgtacggt catcactata ctccacaaac catgtccaac atgactaaag
      421 ttctgactga agaagtaaat gcctttaaat ccagagcctt aaatgataag tatgtcgcta
      481 tttttatgga cgctactta
//
LOCUS       DAZKOR010000238          497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000238 DAZKOR010000000
VERSION     DAZKOR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.252"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>497
                     /locus_tag="ACG8ZM_002943"
     rRNA            <1..>497
                     /locus_tag="ACG8ZM_002943"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          120 c          147 g          111 t
ORIGIN      
        1 ctggtctgta actgacgctg aggctcgaaa gcgtggggag caaacaggat tagataccct
       61 ggtagtccac gccgtaaacg atgagtgcta agtgttggag ggtttccgcc cttcagtgct
      121 gcagctaacg cattaagcac tccgcctggg gagtacgacc gcaaggttga aactcaaagg
      181 aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgaagc aacgcgaaga
      241 accttaccag gtcttgacat cctttgacca ctctagagat agagcttccc cttcgggggc
      301 aaagtgacag gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc
      361 cgcaacgagc gcaaccctta ttgttagttg ccatcattca gttgggcact ctagcaagac
      421 tgccggtgac aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc
      481 tgggctacac acgtgct
//
LOCUS       DAZKOR010000239          495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000239 DAZKOR010000000
VERSION     DAZKOR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.253"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>495)
                     /locus_tag="ACG8ZM_002944"
     rRNA            complement(<1..>495)
                     /locus_tag="ACG8ZM_002944"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           99 a          162 c          106 g          128 t
ORIGIN      
        1 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
       61 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      121 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      181 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      241 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      301 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      361 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      421 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
      481 cccgaaagcg ccttt
//
LOCUS       DAZKOR010000240          494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000240 DAZKOR010000000
VERSION     DAZKOR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.254"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..325
                     /locus_tag="ACG8ZM_002945"
     CDS             <1..325
                     /locus_tag="ACG8ZM_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493802.1"
                     /translation="KASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDLIRMRQR
                     EGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRASLYRKL
                     SEVNN"
BASE COUNT          197 a           66 c           90 g          141 t
ORIGIN      
        1 aaaggcaagt ttaaaatcac taaaagatac atggcttgat ttatcagaag ataatccata
       61 cagccaattc ttaattactg taatggctgg tgttaaccaa ttagagcgag atcttattcg
      121 gatgagacaa cgtgaaggga ttgaattggc taagaaagaa ggaaagttta aaggtcgatt
      181 aaagaagtat cataaaaatc acgcaggaat gaattatgcg gtaaagctat ataaagaagg
      241 aaatatgact gtaaatcaaa tttgtgaaat tactaatgta tctagggctt cattatacag
      301 gaaattatca gaagtgaata attagccatt ctgtattccg ctaatgggca atatttttaa
      361 agaagaaaag gaaactataa aatattaaca gcctcctagc gatgccgaaa agccctttga
      421 taaaaaaaga atcatcatct taagaaattc ttagtcattt attatgtaaa tgcttataaa
      481 ttcggcccta taat
//
LOCUS       DAZKOR010000241          485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000241 DAZKOR010000000
VERSION     DAZKOR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.255"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..151)
                     /locus_tag="ACG8ZM_002946"
     CDS             complement(<1..151)
                     /locus_tag="ACG8ZM_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0493803.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYES"
     gene            complement(170..331)
                     /locus_tag="ACG8ZM_002947"
     CDS             complement(170..331)
                     /locus_tag="ACG8ZM_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0493804.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          164 a           97 c           56 g          168 t
ORIGIN      
        1 aactttcata taaagccttt tctatttcat tgcttgattt cattattgtt aaacaagaat
       61 acaagcaaga aagaactata gttaatatga taaaaattat taaagttcta tttttctttc
      121 ttgtaatata tgctatagca ttttttatca ctttcctaac tactcctttc tattttctta
      181 ctccataact aattgcatcc actggacaag catttttaca tcgtccacat ctgatacact
      241 ccagatgatt gcaattctca actgaatctt tattcatttg gcaaactttt ttacattttc
      301 cacaattaat acacttatcc ttatcctgaa tcggctacac tagactagac agaaaaaata
      361 aggtgtgtag aataataaaa aacacactgg aggatttttc atgccaagac gtcaacgaag
      421 aacctattca aaagaattca aacaacaaat cgtcgatctc tatctcgctg gtaagccttg
      481 cgcag
//
LOCUS       DAZKOR010000242          420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000242 DAZKOR010000000
VERSION     DAZKOR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.256"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..216
                     /locus_tag="ACG8ZM_002948"
     CDS             <1..216
                     /locus_tag="ACG8ZM_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFQ0493805.1"
                     /translation="YEFVGMSVRKNGRLVIKYAKDNHAKRFMREENRKIDQKEFRQQY
                     EEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            233..385
                     /locus_tag="ACG8ZM_002949"
     CDS             233..385
                     /locus_tag="ACG8ZM_002949"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493806.1"
                     /translation="MDVVKFLDDADIANDPNNPPQFTCEKCGKEMYPEYYRNALGIEF
                     KISDVQ"
BASE COUNT          164 a           56 c           95 g          105 t
ORIGIN      
        1 tatgaattcg ttgggatgag tgtgcgcaaa aatggacggt tagttattaa atatgcgaaa
       61 gataaccatg caaagcggtt tatgcgagag gagaatcgaa aaattgacca aaaagaattc
      121 aggcaacagt atgaagaaga aaaagaaaaa acttttaaag caattcgctg ggattgggaa
      181 cgccaacgtg aaatttatat gtctaagttg tggtgaaaaa gaatcaatcc caatggatgt
      241 agtaaaattt ttagatgatg cagatattgc aaatgatccg aacaatcctc cccaatttac
      301 ctgtgaaaaa tgtgggaagg agatgtatcc agaatattat agaaacgccc ttggaataga
      361 gtttaagatc agtgatgttc aataagaagg ttaatcatac aaaaagtcca ccggagaatt
//
LOCUS       DAZKOR010000243          375 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000243 DAZKOR010000000
VERSION     DAZKOR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.258"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8ZM_002950"
     CDS             complement(<1..132)
                     /locus_tag="ACG8ZM_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0493807.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DAZKOR010000244          371 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000244 DAZKOR010000000
VERSION     DAZKOR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.259"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>371
                     /locus_tag="ACG8ZM_002951"
     CDS             <1..>371
                     /locus_tag="ACG8ZM_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0493808.1"
                     /translation="SDADKYNPEGQKVTTELNKEPDASEGIKNKEDLPKDTKYTWKEK
                     VDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDL
                     TDNVTNLPTLPEGTTVTDVTP"
BASE COUNT          150 a           58 c           84 g           79 t
ORIGIN      
        1 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
       61 accagacgca tctgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      121 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      181 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      241 tgacgccgat aaatatgagc caacagtaga aggcgaaaaa gtagaagttg gaggcacagt
      301 agatctaaca gataatgtta ctaacttgcc aacattacca gaaggaacaa cagtaacaga
      361 tgtcactcct g
//
LOCUS       DAZKOR010000245          367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000245 DAZKOR010000000
VERSION     DAZKOR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.260"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>367
                     /locus_tag="ACG8ZM_002952"
     CDS             <1..>367
                     /locus_tag="ACG8ZM_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFQ0493809.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGER"
BASE COUNT          137 a           61 c           80 g           89 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgag
//
LOCUS       DAZKOR010000246          365 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000246 DAZKOR010000000
VERSION     DAZKOR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.261"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>365
                     /locus_tag="ACG8ZM_002953"
     CDS             <1..>365
                     /locus_tag="ACG8ZM_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0493810.1"
                     /translation="ESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDY
                     CRLYVGLVDDANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKC
                     YTILLLLSRIRKIIKNMTR"
BASE COUNT          152 a           35 c           66 g          112 t
ORIGIN      
        1 agagtctatt acaaaaagtt tatctccaaa taagcagacg gaatttaaag aatttttaat
       61 aactctaaaa gctatagcat cgaaagaaga tttagaatgt caaatcagag caaaacgtat
      121 ggcaattgat tattgtaggc tttatgtggg cttagttgat gatgcaaatt tgggaattaa
      181 agagaaaaga aagtatataa aaaaatttat tgaagatgag ggattaattc aatgttggaa
      241 gggatatcct acaaatacgc taccatttca acaaaaattt tttcataaaa tgcttgaatg
      301 taagtgttat acaatattgt tgttgttaag tagaattaga aagataatta aaaatatgac
      361 cagaa
//
LOCUS       DAZKOR010000247          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000247 DAZKOR010000000
VERSION     DAZKOR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.262"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>362
                     /locus_tag="ACG8ZM_002954"
     CDS             <1..>362
                     /locus_tag="ACG8ZM_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0493811.1"
                     /translation="IIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSS
                     NLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKK
                     SYFYGLKIHMIVTKTGFP"
BASE COUNT          116 a           64 c           62 g          120 t
ORIGIN      
        1 tgattattgc ttgtgttata tggggcataa ttaatggcta tactagccaa agagccacgt
       61 atagagcggt ttgttccgtc ttatttccta atggtgactt tcctagtagg agtcgattca
      121 ctcgcttaag ttcaaactta gcttacacta tcaagattat tcgatacttt ttcatcaaga
      181 agctcaccaa aggtgaacta gtcggaatta tagatagttt tcctagtcca ttatgtaaac
      241 cagttagaaa tagacaagca aaactattga atcaaatagc caaagttggc tataactcaa
      301 caaaaaaatc ttatttttat ggtcttaaaa ttcatatgat cgttactaag actggctttc
      361 cg
//
LOCUS       DAZKOR010000248          352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000248 DAZKOR010000000
VERSION     DAZKOR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.263"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>352
                     /locus_tag="ACG8ZM_002955"
     CDS             82..>352
                     /locus_tag="ACG8ZM_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0493812.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRY"
BASE COUNT          125 a           55 c           73 g           99 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta ta
//
LOCUS       DAZKOR010000249          335 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000249 DAZKOR010000000
VERSION     DAZKOR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.264"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          125 a           54 c           59 g           97 t
ORIGIN      
        1 ttaagattaa tgacactaaa ttaacagact atacgtagta tctatttgct atgaccatat
       61 cttatatcca taagtcaact cagccggact aaaagggcta atcttactag aacacaagct
      121 gcacttgata tgcaaattaa ccctgaatcg gaaaggtgct taaattggat attaacggag
      181 ggataaagta aaatttatct ctagggaatg tttagaaagg atccaagttc cttaacaagt
      241 gacaattagt aatgtagtaa caacgatagg ttgtatgccg aacatttcaa tttgatacgt
      301 ataaaagacg atttcaagct catccgtgat aaata
//
LOCUS       DAZKOR010000250          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000250 DAZKOR010000000
VERSION     DAZKOR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.265"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG8ZM_002956"
     CDS             complement(<1..>321)
                     /locus_tag="ACG8ZM_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0493813.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZKOR010000251          314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000251 DAZKOR010000000
VERSION     DAZKOR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.266"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..>314
                     /locus_tag="ACG8ZM_002957"
     CDS             15..>314
                     /locus_tag="ACG8ZM_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0493814.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
BASE COUNT          112 a           34 c           59 g          109 t
ORIGIN      
        1 gaaggagtat aaaaatgaac aaacataata gaaaaataac gaataacaaa gaactgatct
       61 tgttactaat agggatttgg ggtatctttt tactctttaa tatgtatatg ccgactatga
      121 gagcggatga cgtagtttat gcaagtagat tagatgaact tggctatttg ggtgcttcga
      181 tagaacatta taagacatgg agttcaagaa ttattataga actattctta atgttctttt
      241 ctaaacactt tatgctatgg aaattattaa attctacgat tatgttagga attgtagtat
      301 tactttgcaa gtat
//
LOCUS       DAZKOR010000252          295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000252 DAZKOR010000000
VERSION     DAZKOR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.269"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          105 a           51 c           43 g           96 t
ORIGIN      
        1 tctataattt ctaggactga tggaaatttt ctatctgtcc tagttttttt gtaaaaaaac
       61 aaaaagacat acaaaatttc cggtagaata gagtctccta caacatcatt ctgggggaat
      121 tcttgtatgt ccatgaatca ttttatcaaa caactcttta ctattaaaga ctcaaatatc
      181 caaattcaat cctttgaaga aactttttat aaaagcagaa aaagtatcgt tattgaggga
      241 actctgatcc gtacctacag acgttgtccg tgttgtaaag attctacaaa gcaaa
//
LOCUS       DAZKOR010000253          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000253 DAZKOR010000000
VERSION     DAZKOR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.270"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>291
                     /locus_tag="ACG8ZM_002958"
     CDS             <1..>291
                     /locus_tag="ACG8ZM_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0493815.1"
                     /translation="AIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVK
                     KAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT           93 a           47 c           59 g           92 t
ORIGIN      
        1 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
       61 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      121 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      181 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      241 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt a
//
LOCUS       DAZKOR010000254          281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000254 DAZKOR010000000
VERSION     DAZKOR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.271"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..>281
                     /locus_tag="ACG8ZM_002959"
     CDS             28..>281
                     /locus_tag="ACG8ZM_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0493816.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           84 a           53 c           56 g           88 t
ORIGIN      
        1 tgaaaacgaa tgcaaggagt gatagctatg atgtctaaaa acaatacgaa tggtagaaat
       61 caatttgcga tgcttacgat tgatgatcta gttcctcaag atcatctcgt tcgaaagatt
      121 gatgccgcgc ttgattttga atttatttat ccaatcgtcg aagccactta ctccgattta
      181 ggtcgcccaa gtatcgatcc agtgattctg atcaaattgg tgtttattca gtatttattc
      241 ggtatccgtt ccatgcgaca aaccattaag gaagtcgata c
//
LOCUS       DAZKOR010000255          272 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000255 DAZKOR010000000
VERSION     DAZKOR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.272"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>272
                     /locus_tag="ACG8ZM_002960"
     CDS             <1..>272
                     /locus_tag="ACG8ZM_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFQ0493817.1"
                     /translation="YIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAFLKRLVK
                     QFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQ"
BASE COUNT           96 a           47 c           55 g           74 t
ORIGIN      
        1 gtacatcaaa attaaaggaa aatggcatta tttgtatcga gccatcgatg cagatggttt
       61 aaccttggat atttggttac gtaaaaaacg ggacacacaa gcagcctatg cttttcttaa
      121 gcggttagtg aagcagtttg atgaaccgaa ggttgtagtc acagataaag ccccctctat
      181 tacaagtgcc tttaagaaac taaaagaata cggcttttat caagggacag aacatcgtac
      241 cattaaatac ctgaataatt tgattgaaca ag
//
LOCUS       DAZKOR010000256          271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000256 DAZKOR010000000
VERSION     DAZKOR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.273"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..>271
                     /locus_tag="ACG8ZM_002961"
     CDS             111..>271
                     /locus_tag="ACG8ZM_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0493818.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALE"
BASE COUNT          102 a           51 c           42 g           76 t
ORIGIN      
        1 accacgggtt tttattacgc cgattgtaaa attaagctag acaactaaaa aagtcatttg
       61 tgctacactc aaaatagttc cgaccaaaga attataagga gtgagtacaa atgacctata
      121 cccatcttac aacggatgaa cttgtaataa tagagtctta tttcaaaatg aatcaatctg
      181 ttgctaaaac tgcccattgc ttgaatcgtt caagacaaac gatccataaa gtatatctgt
      241 tcttcaagca aggaaaatca gccttagaat a
//
LOCUS       DAZKOR010000257          266 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000257 DAZKOR010000000
VERSION     DAZKOR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.274"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..>266)
                     /locus_tag="ACG8ZM_002962"
     CDS             complement(34..>266)
                     /locus_tag="ACG8ZM_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0493819.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 agaaggactg accttctgtc tcagattttt ttgttatttc acttgaatca attgattgat
       61 caaaaaaatc ctgattttca tgttctcaaa tgatttaaat ccataagata atcgtttcag
      121 tgtcttgata tggggaatct tggcctctat tttgccatta gaatacgggt aaatcatagc
      181 attgtaaata ccttcttcat acgttaaaag gttttgaagt ttcgtgcgaa gttgtgcatc
      241 aagtgtttca ggaagatttt ttaata
//
LOCUS       DAZKOR010000258          264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000258 DAZKOR010000000
VERSION     DAZKOR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.275"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           98 a           43 c           49 g           74 t
ORIGIN      
        1 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
       61 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      121 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      181 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      241 ttttgataaa aaacagtggg ttga
//
LOCUS       DAZKOR010000259          263 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000259 DAZKOR010000000
VERSION     DAZKOR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.276"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          108 a           38 c           44 g           73 t
ORIGIN      
        1 tataaaagag tttaaagcgt aatacactta tggggaagtg gtttggggtg cgctttaaac
       61 tcttctttat tattatctca cagcatgctt aaaaggtcta tggattaagt tatgtaaaaa
      121 agtgcctctt taaacgtcct aaaatccaaa caaggatagc ataatgtaca ctatcgttga
      181 tccagaaaac aggtaaataa attttgcaaa agcaataagt taaaaacatt acaaaaaaga
      241 caaggatatc acatcaagaa aaa
//
LOCUS       DAZKOR010000260          247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000260 DAZKOR010000000
VERSION     DAZKOR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.277"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          116 a           30 c           37 g           64 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaa
//
LOCUS       DAZKOR010000261          241 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000261 DAZKOR010000000
VERSION     DAZKOR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.278"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..220)
                     /locus_tag="ACG8ZM_002963"
     CDS             complement(<1..220)
                     /locus_tag="ACG8ZM_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0493820.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT           63 a           45 c           46 g           87 t
ORIGIN      
        1 caggaaaaat gatttcggtt ctcccacaac gtcgcttatt ttctttgtat tgttcaaagt
       61 attcaagtgc tgttccacca catttgagaa agttatagac attgtaaatt gtttggcgtc
      121 cacgtttaag ctgcttcgca acgatagcaa ccggagtttc ttgatgaaaa tatgcttcta
      181 tcattacaag ctcgtttggt gtaagatggg tataagtcat ttatgttcac tctccttgta
      241 t
//
LOCUS       DAZKOR010000262          233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000262 DAZKOR010000000
VERSION     DAZKOR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.denovo.280"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAZKOR010000263        15446 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.guided.065, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000263 DAZKOR010000000
VERSION     DAZKOR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.guided.065"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..482
                     /locus_tag="ACG8ZM_002964"
     CDS             <1..482
                     /locus_tag="ACG8ZM_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0493821.1"
                     /translation="AKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIGN
                     GTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSAL
                     AESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP"
     gene            complement(745..1212)
                     /locus_tag="ACG8ZM_002965"
     CDS             complement(745..1212)
                     /locus_tag="ACG8ZM_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0493822.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            1335..2186
                     /locus_tag="ACG8ZM_002966"
     CDS             1335..2186
                     /locus_tag="ACG8ZM_002966"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0493823.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(2437..4395)
                     /gene="nagE"
                     /locus_tag="ACG8ZM_002967"
     CDS             complement(2437..4395)
                     /gene="nagE"
                     /locus_tag="ACG8ZM_002967"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0493824.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(4392..5264)
                     /locus_tag="ACG8ZM_002968"
     CDS             complement(4392..5264)
                     /locus_tag="ACG8ZM_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0493825.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(5606..6478)
                     /locus_tag="ACG8ZM_002969"
     CDS             complement(5606..6478)
                     /locus_tag="ACG8ZM_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0493826.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            6606..7175
                     /locus_tag="ACG8ZM_002970"
     CDS             6606..7175
                     /locus_tag="ACG8ZM_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0493827.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            7242..7979
                     /locus_tag="ACG8ZM_002971"
     CDS             7242..7979
                     /locus_tag="ACG8ZM_002971"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0493828.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            8210..9478
                     /locus_tag="ACG8ZM_002972"
     CDS             8210..9478
                     /locus_tag="ACG8ZM_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0493829.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            9965..10429
                     /locus_tag="ACG8ZM_002973"
     CDS             9965..10429
                     /locus_tag="ACG8ZM_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0493830.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            10413..11042
                     /locus_tag="ACG8ZM_002974"
     CDS             10413..11042
                     /locus_tag="ACG8ZM_002974"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0493831.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            11396..12874
                     /gene="msr(C)"
                     /locus_tag="ACG8ZM_002975"
     CDS             11396..12874
                     /gene="msr(C)"
                     /locus_tag="ACG8ZM_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0493832.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            12921..13367
                     /locus_tag="ACG8ZM_002976"
     CDS             12921..13367
                     /locus_tag="ACG8ZM_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0493833.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(13566..14081)
                     /locus_tag="ACG8ZM_002977"
     CDS             complement(13566..14081)
                     /locus_tag="ACG8ZM_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0493834.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            14198..15400
                     /locus_tag="ACG8ZM_002978"
     CDS             14198..15400
                     /locus_tag="ACG8ZM_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0493835.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4835 a         2783 c         3209 g         4619 t
ORIGIN      
        1 tggcaaagta taggatcgaa gaaaaaccag attttacggt gacgggtatt ggtgtggaat
       61 tgaagagtag caatacggat tttgtcggtt tggctgcaga gaaacaagca tttgagcaaa
      121 aagttatcgg taatggaaca atagatgcat tgaaacaagc tgctgaaaat cattatttct
      181 ttacagtcaa cgaaccctat aacgggaaaa tgatgtacta tgtcggtgtt caaacgagtc
      241 aggatgttcc aaacactacg cgaatcatcc cattcccaaa aggaacgtat cttgttgtaa
      301 gcgctttagc agaatcgcct gaagaattaa gaaatgattt gacgaatacg gcctttgggc
      361 atgttttaag agaagtagaa gattacgcct atgttggggg tccaaatgct gtagtcgaaa
      421 tgggagttac caacgggaaa tatatgggag aaatctggat accggtagtc aataaaccat
      481 gataatatag aggtcgtgaa agtagcgctt tgacctgagc attagagagg aaaaatggga
      541 aatagctttc catattttgc atttttcaga actaatgccg agggtcagct acttgaacac
      601 cgtttatcaa gacgtcatga cttcagcgct ttgacctgag cattagagag gaaaaagggg
      661 catgacaagg cttttgtccc ccggagaata aaagaattta tgtagacgat tccgatcaga
      721 aaaatgcagg cacagtagag agaattataa aaatctctct acgaggcctg tgttttctgg
      781 tcttttttct ttttccaaaa cggctatttt atgtatcaga ctatcaagtg tgatcttagc
      841 catttcttct tctgcctttt tctgcagccg agcgtagctt tcttctaaag cttcttggat
      901 attcgcacca acgacgcaat ttggatttgt ttttggatcg acttggataa gctgtgtatt
      961 accttcgatg ctgcgataga catctagtaa agaaatctga tctggacttt tggcaaggga
     1021 aggctgcggt tttccggttt ttgtaaagat caaaccggat tttttgagct gactcatgat
     1081 tttacgtaca tttgctgcat ttgtttcgat actactggca atcatctcac tagaaagtga
     1141 tcgggtatct tgaaacattt caatataggc aagtacatgg attgcatcac taaattgcag
     1201 agaatacttc atcagttgtc atcctttcat tttttttgct tgacttatct taccacactc
     1261 tttatgataa aggtgtaatt tataaatgta cagctgtaaa tcttaacaaa taaaaagaat
     1321 agggagtggc aaatatgcat tatttagtaa caggagcaac tggaggtttt ggtggttatg
     1381 cgctggaatt cttaaaaaag atggttccac aaaatgaaat ctatgtacta gtacgtagtg
     1441 aagaaaaagg agaagcactg aaagaagctg gatttaatat acgtattggt gattatgatg
     1501 atttagactc aatgaaagaa gcgttgaaag gaatagatcg attgcttttt gtctctggcg
     1561 ttccaggaaa tcgtcaagcc gaacataaga atgtagtgaa ggcagcacaa gcagctgggg
     1621 tatcttatat cgcttataca agttttgcag atgccgatca ctcgatcagt gtattagcgc
     1681 cagatcacca attcactgaa aaaatgatta aggattcagg tatttcccat acatttttac
     1741 ggaataattg gtatgtagag aatgaattac ctatcattgg gcaggcttta aaaactggtc
     1801 aattcgttta tgctgcagga aatggaaaaa caggttgggc attgaaacgt gaatatgcag
     1861 aagctgcagc aaaagtacta gtagaaagtg actctccaga aatcttagaa ttatccggca
     1921 atctaactga ctatgaagaa ttagccaagg cattagagcg tgcgacaggt aaagagttag
     1981 aaattattga agcaagtgat gcagcgtttg tcgaaaatct aaaagaagcc ggattccctc
     2041 aagaagcagc tgacatgttt ttagcgatcc aacatgatat taaaaaggat cagttagcta
     2101 tttcctctga tgatttagaa aaagtattgg gaaaaccatt aacttctctt gaagcagcat
     2161 taaaagaaat attggatcag gaataatgta taaagaggtt gtgaaaaagc cgattagttt
     2221 tgagtatgaa gaaaaatttt gtgaaaaagc tgttctgcat caagtaataa gaagagtcaa
     2281 ataaaaaata gctcttcata tgtttcgttt agctggactt attaccgcgg agttggcagg
     2341 agaacgccgt ttttattcgg aaaaaggagc tgagacaaga tttttgtctc agctcctgtt
     2401 tgtttatcga taaaaaagtc tggggaagac gatggctcag gctttgttgg aaacagagcc
     2461 gattttatca tttgctttta catcacgtgc gggttcgatt tctaagcttt ggactttgtc
     2521 gccatttgtg aatacaacga ccatatccgt gtttttgcct gcagattgaa tagcggcaag
     2581 atcaactaaa gcaatcaatt gacctcttgc gactttttga ccttcttcta catataaagt
     2641 gaatggttcg ccttttaagt caacagtgtt gatgcccata tgaagcaaga cttcgatacc
     2701 agcatctgtt tggattccga cagcatgttt cgttgggaaa atgtttgtga tcgtaccagc
     2761 gattggagag aagatttctc cattttctgg taatacagca tagccgtcac ccatcatttt
     2821 ttccgcgaat acatcatctg aaacttcagt gattggaatc agtttcccat ttgcgatagc
     2881 gtaaatatct tgttcgatcg tttcaccggc aaatgtttct gttttaacca cggtgtttgt
     2941 ttgtgcaggt cgtgctgcag caccaccatg tagttttgcc atctcatctg caacgaattg
     3001 tacttctgta cctacgatga cttgcgcatt ttttccgtcg atgatcttca taccaggtac
     3061 gccagtggct ttgattttat tttgatcgat caggcctgta tctttgactt gcaaacgaag
     3121 gcgtgtagta cagttatcga tcgaagttac attgtcggct ccgcctaatg cagcgtagat
     3181 tttgcttgct tgtgccgcat atttattgtc tgaattagtc gttgcagtat cgtcatctga
     3241 aagatctgct tcctcgcggc caggagtcat cagatggaat tttttgatag caaagttaaa
     3301 gccaaagtag taaatcactg ctgttactaa tccttggacg atcaacatca atggctgatt
     3361 cgcaatcgga tttttcaaag ataggaagta atcgacaaat ccggcactaa aggcaaatcc
     3421 tgctgtccaa tggaagaatg cactcactgc taaagaaatc ccagtgaaaa ttgcatgcaa
     3481 gacatataat ggccaagcaa cgaacataaa tgaaaattca agtggttctg tcacaccagt
     3541 aaagaatgaa gcaaaagctg ctgctagcat caaggaagct gtttttgctt tgttttctgg
     3601 acgtgcattt ctgtaaatag catatgctcc tgctggaagc ccgaacatca tgattgggaa
     3661 aaatccagct tgatacattc ctgttacgcc ttttgttcct tcagaagcca aaaatttacc
     3721 aatatcattg attcctgcta cgtcaaacca gaacacggag ttcattgctt gatgcaatcc
     3781 tgtagggatc agtaaacggt tgaagaagcc ataaagacct gcaccacccc agcctaaact
     3841 caaaatgaat ttagcaaaag aaactaaagc agagtaaacc ggaggccata cgagcagtag
     3901 cactgcactg atcagcaaca tgacaaaggc agccaagatt ggcactagac gcttgccgct
     3961 aaagaaagat aaagccatcg gtaattttgc ttcgtggaaa cgattataaa gagctgccgc
     4021 aatcaatccg gcaataatac caataaatac gttactgatc tgagcaaaag atggatcaac
     4081 tgattctggt ttgatattca ataaagtagc tactgaatca gtttttaaaa catttactgg
     4141 aacttcaaat gcgaccaaac ctgccaaggc agcagcacct gatttgtcca ctgacatccc
     4201 tagtgctaac ccaacggcaa acagcaaagg taattgacct aaaactgcgt taccgccagc
     4261 ttgtaaaaaa tgagcgacaa tgtcgttact ataagatgcc caccagtttc cgatccctac
     4321 tagtaaagca gcagcaggta agaccgcaac tggaagttga agggaacgtc ccattcgttg
     4381 tagatatgct ttcaattttt ttcctcctca tgattgttaa catttgtttg tacgattgcg
     4441 cttcttaaac gctcgatatg gatggccaag taacccagtt cttctttcgt gatcgcgagc
     4501 tggtattctt tggtcagcaa cacacggatt tttttagaaa tcttatagct ttctgggtat
     4561 tttcgtttga ccatttctac gatttccgtg tctaaaacag catattgttg cgcatgggtt
     4621 cgttgtagaa gcagtcgtaa atgattcacc agtcttgaat aattcagtga aaatggttct
     4681 gaatggatat ctgtttccaa ttctgattcc actaaatgga tgatgtcaga tacaatcgtc
     4741 acttcacgga tactgcgatg gttattgttc cgaccgcttc gtccgctgtg aatatggatt
     4801 gcgatatatc ctgcttcgtc ataagtatat ggaatcccaa gctcttttgt cagatactct
     4861 actgcccatt gagcaatact gaattcatca ctgtaaagca cttcgatttc tcttaaaagt
     4921 ttgttgcgga tgacgatacc gttattaatg ttctccgcag aaaaagcaat gtggtcggtc
     4981 aaagcgatca atacatgttc attcaacttt tccatcaaaa cagtttccgc atgatcaatg
     5041 atcgtttctg ctgcaaataa aaagcgttca tcaatctgat taagtaaatt ttgcaacttg
     5101 atttgacctt ctggttccat gacaaatatc cgttcgacat cacgatcaaa aatcaaatca
     5161 tttctttttc tgtcaaatcc aatccctttt ccaatagcta ctttttcctg tccaccgtca
     5221 tctaccaata ctgcattttg attaaagatc tttttgattt tcatgcgcgc accctttttg
     5281 aagatttggt tttcacgttt gctagaccag ttgattgcgg ccaaacaaaa atagacacga
     5341 aaaacctcct agggaaacca tgttcctcag agatttttca tgcctagtat gatctagtca
     5401 catgaaacga attattgtta acgtaatcaa ttttatatag acagttatgt tttgtcaatt
     5461 ctttttttat aattatagta atttactgga attatcagaa ataaaaggaa gaaataaatt
     5521 aagaaactta tgttatttaa tcgttggatc ttttgcttat cttattcgtt gttaaaaagc
     5581 tgaaagcgaa ggaaaaaaac tctacttaac tgtatttctc cgttaggaca tctaaaaatt
     5641 tacgagcagc aggagaaaga ggctggtctt ttttccaaat gagattcagt gaagcttgta
     5701 atggcggatc gagtgggatg aatatttttt ccgtcccata agtattgatg atcccatcta
     5761 gagctaatgc accaaaagtc ccgttatcga ccatcaaaga agcattgtac aataagttat
     5821 atgttccgac gatcgataac tgatctatgc tgaaacctag ccattcagct agttgactgt
     5881 ccactgacgt ttgccgggaa atgatcaaag gaacagtttt cagttcctgc ggatggatga
     5941 cggtttgctt tgctaaagga tgatttcgat gtatcaaaag tccccaacga tcttgggctg
     6001 gcagtcgaaa tgactggtat ttttgtttgt ttgtcggttc gattacgagt ccaaaatcaa
     6061 gtaatccgct atccagtttg tctatgatat catctgcatt cccgctatat aaatgaaact
     6121 ggatatcagg atgcgccttg atcaacgaat gaaggctatc agaaatgaaa gagattgctt
     6181 tagtttcgcc tccgccaatg tagatttctc cagagatgat tgcgtcattt gttaaattgt
     6241 tcgccgtttt ttctaccaac gacaagattt cttttccttt tgaataaaga tatcgaccag
     6301 aatccgtcaa ctgtatctct cgggagcccc gagtgaaaag tgtcacacca agttcttctt
     6361 ctaaatcttt cagttgtttt gagagagtag gttgcgtgat atgcagagat tttgctgctc
     6421 ggctgatatt tttttctcta gctactgcta aaaaatacgt cagtatacga atatccattt
     6481 tttccctcct gtttttaaaa aagcatacca ctaagtatag ttaaaaggaa tagttaagta
     6541 tgttttataa gtattagaca tgataagccg tccgttataa agtaaggata aaaggaggga
     6601 gcaaaatgac aagcctcatc gaaaaacaaa tcgtcaataa agagattcga gtaacggatc
     6661 cgctctttga agtgatccat cagatccaag ctgaaaacga agagccttta gcagcgctga
     6721 atactgggta ccatgaacca gcagatatcc gcaaacggct agaaaatatc attagtgaca
     6781 aagtagatga tacggtgact gttctgctgc ctttttatac agatttcggc aaacatatta
     6841 gtatagggaa aaatgtcttt atcaatcgtc aagtgatgtt cgttgactta ggtggcatct
     6901 gtttagaaga ttcagtattg attggtcctc gtgtcaacct catcacggtg aaccatctaa
     6961 ccgatccaaa agaacgaaga ggcttgttag taaagccaat ccacatcaag aaaaatgctt
     7021 ggatcggagc aggagcaact attctgccag gcgtaacgat tggcgagaat gccattgttg
     7081 cagcgaatgc aactgttacg aaagatgtcc cagacaatac catcgttgca ggaatccctg
     7141 ccaaaatcgt caaacctgtc gaacggatta tttgaacagt aagatgaaat aagagatagt
     7201 gagtataagc aagagatttt tcaaaacagg aggagaatag aatgaatgat ttatcagaaa
     7261 aagtgattgt catcatgggc gcttcaagcg gaatcggtga agcaacaacg aagctattag
     7321 cagaaaaagg ggctaaacta gttatagctg cacgccgaga agatcgccta aaagcaataa
     7381 aagaatcttt accagaagct gagctatata ttcaaacagc agatgtaaga gattttgcac
     7441 aagtccaagc agttatcgat ttagcgatgg aaaagttcgg tcgaatcgat gtattgtaca
     7501 ataatgcagg aatcatgcca accgctccat tagttgaagg tcaccgtgat gaatggcaaa
     7561 atatgcttga tatcaacatc atgggtgtgt taaatggaat ttccgccgta cttccaatca
     7621 tggaaaaaca aaaatctggg catatcattt caactgattc tgtggcggga catgttgttt
     7681 atcctgattc cgccgtttat tgcggaacaa aatttgctgt ccgtgcaatc atggaaggat
     7741 tacgtcaaga gcaacggcaa aataacatca aatctacgat catctctcca ggagctgttc
     7801 aaactgaatt atatcaaacc atcagcgaca aaaaggctgc tcttgaactt catgaagcgc
     7861 aaaaagaatg gggattgacc tccgaagata tagcttctgc tgtagcattt gcgatagaaa
     7921 cgccagaccg gatgtctgtc agtaacatga ttatccgtcc gacgacacag gaagtataat
     7981 ccataacgga taatatccaa agagaattac ttgttaaata gttattcaaa cgaccatcaa
     8041 tttaaggcgt gaatggagaa gaatattctt catttacgtc tttttatgta gaaaaagcaa
     8101 atttatcaaa aagaaacaaa tgttatttaa atatggacat tgacttgctt ttactttttt
     8161 agttacactg ttatttagga aaagtttcat aattcaaagg agaaaaaaga tgaaagcaaa
     8221 gaatttatta tttagcgtta ttcttttagg aaatctagca gttgccaaca caacagtgct
     8281 agctgaagaa tcgacagaaa ctgtcgcaac tcagacatcg agttcttcag aaacaggaat
     8341 tgaaaacgat acagagagta ctttgcagtc agctactgtc tcagaaaata caatttcaac
     8401 cgaagaacca acaactgaaa caacagaaca aacacaaacc gaagacagta catcagcaga
     8461 agctcagaac aatccagaga atgcagaaga aatcacgaac caattgacag atgggcaaac
     8521 attgacacat acccatcaag cagatttgtc tgaattcgaa aactatactg gctatgcttt
     8581 ttatgctcgc tcatctgcta attctcaaca agcgttcatt gatagtattg cctcaactgc
     8641 acaaagtttg gcaagtgcca atgacttata tgcatcagtc atgattgctc aagccatcgt
     8701 tgaaagtggc tgggggaaca gtactttggc ttctgcccct aattacaatc tatttgggat
     8761 taaaggaagc tataatggcc aatctgttac gatgcctact tcggaatatg tgaatgggca
     8821 atggattacg gtgaatgccg cttttagaaa atatccttcg tacaaagaat cattgcaaga
     8881 taacgtaacg gtattgaaga cgacttcttt tcagccaggc gtttactact attccggtgc
     8941 atggaaaagc aatacgaact cttacaaaga tgctacggct tggctaacag gtcgttatgc
     9001 cactgcgcct aattatggtt ctacattgaa caatgtaatc gaaacctata atctgacaca
     9061 gtatgataca gcaccaactt caacttctcc gatgtatcgt ctgtataacc gtcatacagg
     9121 agaacattta tatacattga atgccggaga aaaaaattat ctgccaactg taggctggga
     9181 atatgaaggg attgcttggc aagcaccaaa cagcggacaa cctgtctacc gtttatacaa
     9241 tccttacagt ggagaccatc attacacaat ggcacaatct gagatcaact ctttaacaaa
     9301 aatcggttgg cgttatgaag gtctaagttt ttattctggt ggttccaagc caatttatcg
     9361 actatttaat ccaaatgaaa aaactggtac ccatcactat accttgagtg ctaaggaaag
     9421 agattggcta acaccgatgg gctggagata tgaaggaata ggattttatg gttattaaaa
     9481 agaggtagtg aaaaagctgc cctgcatcaa gtagtaagaa cagccaaaca aaaacagatt
     9541 ttcatgtttt ttgtttggtt gaacttatta ccgcagatgc aagtttttga acaccgttta
     9601 ctcaaaaaag gttgtgacaa catcttgtca taactttttt attactgttt aagcatactg
     9661 atccaatttc taatattcga cgggattctt gcaaaaggcg atttcggaaa tttttggcgg
     9721 tgtaaaattt tctactgcag aagagctatc ttctatagtt tgataagaat caataatctt
     9781 ggttttagcg ttgtgctttc tttatcattt tttccgttca ttctacaaaa aatttctttt
     9841 ctctatactt ttctattgag agcgtttttt ttattacaca gctgattctt tgactttttt
     9901 ctttttttat tctattattg acaagtcaat cgttgacgag tcaattatta gaaaggagga
     9961 aattatgtct ttagaagaga ttagtcacct tttatatcaa atcaaattag ccgatcaaaa
    10021 aatcgtccag atctttgaag aaaatatcgg tataagtctt actagatacg agatcatgat
    10081 gagactgaaa gaagaaaatc cattgctaca aagcagtttg caagaaaagt tgcagattga
    10141 tcaaggagca gttactcgtc atctgaaggt attggaagca aatggctatg tttcgcgtca
    10201 gagaaatcct cagaacaacc gggaagtgta tgtagcgcta acagaaaaag cccagaagaa
    10261 aatcgagcat tgtgaaatgg accataaaga tacaggttcc gtgctatccc cggctttcag
    10321 cgatgtagaa atgaaacaat ttctaacact tttagataaa ttgaataccc aactttgcaa
    10381 tataacaaca gaaacgaaag aggttaataa taatggaaca aaaaaatgat ttttcaacaa
    10441 ttatgactgg tagaagatcc gttcgcgtat atgatgaaac atacaaaatc ccacacgaag
    10501 aaatgctgga aatgatcaaa gaagcgacga ctgcaccttc ttctgtcaat atgcaaccat
    10561 ggcgctttgt ggtcgctgaa agcgaggaag caaaagaaat cttgcgtcct ttgatccgtt
    10621 tcaatacgag acaaaatgat acttcttctg caatggtcat gatttttggc gatatgcaat
    10681 gttacgaata tggcgaagaa atctataacc aagcttacga atcagggaag atgccaaaag
    10741 aagtcagaga tcaacaatta gctgcgatca ttccttatta caagagtttt tcaagagaag
    10801 aaatgaacga tgtagtcaaa gtcgattcta gcttagcggc tatgcaattc atgttagttg
    10861 cgcgtgatca tggctatgaa acaaatccaa ttggtggagt cgaagcagat cagttggcag
    10921 aggcatttgg tttggataaa gaccgttatg ttccagttat tattttatca ataggaaaag
    10981 cggtagaaga aggttacgag tctgttcgtt tagcaccaga aaagatcact acgttcgaat
    11041 aaaaggtttt cagttactat tcagtagcta ttgctttttt tgaatcgtgt gtggtaacct
    11101 aataaaaaag gaggtcggaa aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa
    11161 taacaactaa ataaattcaa aagacatgta taaacaacaa tttcatggat ggagtatgtc
    11221 cattttttcg aacgacagta gaataggtga aagctatgac agatttggtg atttagtcat
    11281 agttatttcg ttcagagtag gaaaagatgt gatgcagttt ttttgcaaac agatttttct
    11341 gctctttttt agtatcgata attgttgttt tacatgaaat aggagtgtaa aacatatgga
    11401 aaatttagca gtaaatataa caaatctaca agtcagcttt ggcaaccagc tagaactatc
    11461 tattgattct cttcgtgtct atcagcaaga tcgtataggg atcataggag aaaatggtgt
    11521 cggtaaatcg actttgctca aactaatagc cggtgaactt tttcccgatc atgggaaaat
    11581 ccaaacagag atcaccttca actacctgcc tcaattaacc tatctcgctg aagcaaagga
    11641 cctaaatttg gaattagcca gtcatttcca gttaagactg gaagaaactt cagagcggaa
    11701 atggagcgga ggggaagaac gaaagatcga gttgatacgt cttctttctt cttatgaaca
    11761 agggatgctt ctagacgagc caacaaccca tctagataga aaaagtattg atcgactgat
    11821 tgaagagctt cgttattatt atggtacgct ggtttttgtt agtcatgatc gctattttct
    11881 agatgagcta gcatcgaaaa tctgggaagt aaaagacgga gaaatccgag agttttcggg
    11941 gaattatagt gcctatctca ctcaaaagga attggagaaa aagactcagc tacgagaagc
    12001 agagtcgatc atgaaagaga aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca
    12061 agcggaaaag ttggaaaaag tgtccagtaa aaagaaaaag caacaaatca gaccggatcg
    12121 gttgtcttcc tctaaacaaa aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac
    12181 attagagaga agactccaaa aaataggaga aacaaccaag ccgcagcaga tgaaacaaat
    12241 ccgttttcca gtaccaaaat ctcttgaact ccacagccgc tatcccatca tgggacaaaa
    12301 tgtccaattg gaaagaagtg ggagaacatt actggtaaat ggtgattttc agttttcttt
    12361 aggtaaaaaa atcgcgattg tcggtgaaaa tggttcaggt aagacaacct tattagaaca
    12421 tatccgcaaa caaggagaag gaatccttct ctctccgaaa gtaagctttc aagtatatca
    12481 gcaaaagggt tatcaaatga catctgaaga atccatcatt cgttttgtca tgagacaaac
    12541 agagttttca gaatcgcttg tccgtagttt gctgaatcac ttagggtttg ctcaggaaac
    12601 tttgacgaaa ccgttatgta cattaagtgg gggagaagcg acccgtctga cgattgcttt
    12661 gctttttact aagccaagta atgtgttgct gttagatgaa ccgactaatt ttattgatat
    12721 ggcaacgatc gaagctttag agaagctaat gcaaatatat ccgggaacga ttttgtttac
    12781 ttctcatgat tcttactttg tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca
    12841 gaaaataaaa aaagtactta cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa
    12901 atttaaagga gtggtacaga ttgcctacaa ataaaaaaga aggaataatt tttacaacaa
    12961 tgatgtgttt tttgatggtc ttggggatga gcgcgtataa tttatggctg catcaagatc
    13021 ttcatttatc aagtttgttg attggtctag tgcctggatt cgtggtggct ttcattttgg
    13081 atgtcttcat catcggtgtg ctggcgaaaa aaattgcctt taaactacca gtaaatccag
    13141 aaagcaaggt ccaaatgatc ttaactattt catgcttgat ggttttagga atggtgacat
    13201 gtatgtcttt attcggtgtg atcatggaac aaggaatacc tgatcaatta ttctctgctt
    13261 atatgaatgc ttggaaaatg aatgtgattg ccgctttacc gttgcagttg atcatcgttg
    13321 gcccgctttc aagaagtgtg ctaaaagcaa ttcaaagtaa tgaatgatgg aacactgtta
    13381 tttgaaaaaa ggccttggca tgctttttgt catagtctct tgaatgcagt tctagaaaaa
    13441 tagtgatccg aacgataggt tttggcctgt cgttcggatc gctttttctt ttccaactgt
    13501 attgctcttt tattgaagat aatttgctct tcaatagata tttctagaaa ggttctgttt
    13561 atcccttact caaaaaactg ctgcatttgt tctcttactt gctgacacca ttggagttcc
    13621 tgctgacaaa gcataattct gcgctggatc acaacataac gtccaaattc ttgtttcttg
    13681 tctagcgaga ggtcattcaa ttttttttga tttttttgca tgattttgtt cagctgttgt
    13741 tctctagtga atagcagtcc agtagctgtt gttttatcga gcgtcgaaaa agcatagatt
    13801 tttgctagaa attcatcttt ttgcgtagga gcgggagttt cctctttgat ccattcctct
    13861 acaagagcac gtccttcatc agtcaagtgg tagatctttt tttgagagtg ctcgtctttg
    13921 tccaaaacgg tcacataacc ctgctggtct aacttggcaa gcgttgtata gatctgactg
    13981 tgatgggctt cccaaaaaat tgtgatcagc tgcttcaatt catacccaga acgtggtttg
    14041 gataaaagca cgcataataa tatataggct aaagtattca taaaaatcct acctctcgtc
    14101 ttattttctt tattatatca cagtaaagtt tccacgtttt acatgtgcat atttacatgt
    14161 aaaataaatc atgtatgatg ctaatagaga gaaagggatg gaaatattgg agaatcaaat
    14221 agatatacaa gaacaaacaa atgaagaaac aaaggggaaa agttcagcgc tagttgtagc
    14281 agctacctgt atgatcgctt ttatgggagt gggtctagta gacccaatct taaaaacgat
    14341 tgctgcacag ttaaacgcta cgccagctga aacgaccctg ttatttacta gttacatgtt
    14401 agtcactggt gtggtgatgc tatttacagg tttcttatct agtcggatcg gtttgaaaaa
    14461 aacattgatg acaggattgt ttatcatcgt tgtattcgct ggtcttggcg gattatctgg
    14521 gaatatcgga atgctgattg ggttacgtgc cggctggggt gtcgggaatg ctttatttgt
    14581 ctctactgct ttagcaacga tcgtcagtat tttagttggt aacacagaaa aagcaatcat
    14641 gatgtatgaa gcagcacttg gctgcggaat ggctgttggc cctctagtcg gtggggtctt
    14701 gggcagtggt tcatggcgtt atccattttt tggggtagca tttttgatgt tttgcggttt
    14761 tatagcccta gcggtacttt tacctgaaac tgagaaacca aagagaaaag taggcctttg
    14821 ggaaggtgtc aaagcgttaa gcaatcataa acttcgttca gttggactaa tcgcattgtt
    14881 atataatttc ggattcttta ctttacttgc gtatgcgcca tttttattga ttggctattc
    14941 tgaattagaa gtcggttttg tattttttgg ctggggtgtg cttctggcta ttgcttcgat
    15001 tttcgttgca ccggctcttg agcggaagtt tacgacttat cggacaattt tagcagcatt
    15061 tgtcttattc attatttgtt tgctgttatt aggagctgga acttctatgc cagtccttgt
    15121 accgatcagc attgtgttag caggattttt ccaaggaatt atcaatacac tgttgacgac
    15181 gattgctatg gaaataccag gtctgcaacg aaacgttgct tcgtccagct atagttttgt
    15241 ccgctttttc ggtggagctt tagcaccatt tattgcaggt gtgattggtg aacatattgg
    15301 tgaaagctat gcgttttatt ttgcaggtgc cattgttctt atcagtgtat gttgtatcat
    15361 ttatcaccgt aattattttg ctacaaatga agaagaataa gaaatcagag gttgtgaaaa
    15421 agctgtcctg catcaagtca taagaa
//
LOCUS       DAZKOR010000264        11796 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.guided.090, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000264 DAZKOR010000000
VERSION     DAZKOR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.guided.090"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..182
                     /locus_tag="ACG8ZM_002979"
     CDS             <1..182
                     /locus_tag="ACG8ZM_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493836.1"
                     /translation="ETKSDKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSK
                     HAILKQLNEKANQVN"
     gene            225..1715
                     /locus_tag="ACG8ZM_002980"
     CDS             225..1715
                     /locus_tag="ACG8ZM_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0493837.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            2063..2233
                     /locus_tag="ACG8ZM_002981"
     CDS             2063..2233
                     /locus_tag="ACG8ZM_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493838.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            2247..2864
                     /locus_tag="ACG8ZM_002982"
     CDS             2247..2864
                     /locus_tag="ACG8ZM_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0493839.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            2864..3541
                     /locus_tag="ACG8ZM_002983"
                     /pseudo
     CDS             2864..3541
                     /locus_tag="ACG8ZM_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            3852..4349
                     /locus_tag="ACG8ZM_002984"
     CDS             3852..4349
                     /locus_tag="ACG8ZM_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFQ0493840.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDC
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            4350..4766
                     /locus_tag="ACG8ZM_002985"
     CDS             4350..4766
                     /locus_tag="ACG8ZM_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFQ0493841.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            4786..6330
                     /locus_tag="ACG8ZM_002986"
     CDS             4786..6330
                     /locus_tag="ACG8ZM_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0493842.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            6473..6697
                     /locus_tag="ACG8ZM_002987"
     CDS             6473..6697
                     /locus_tag="ACG8ZM_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493843.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            6712..7581
                     /locus_tag="ACG8ZM_002988"
     CDS             6712..7581
                     /locus_tag="ACG8ZM_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0493844.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            7562..8296
                     /locus_tag="ACG8ZM_002989"
     CDS             7562..8296
                     /locus_tag="ACG8ZM_002989"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0493845.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            8329..9237
                     /locus_tag="ACG8ZM_002990"
     CDS             8329..9237
                     /locus_tag="ACG8ZM_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0493846.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            9234..9776
                     /gene="sat4"
                     /locus_tag="ACG8ZM_002991"
     CDS             9234..9776
                     /gene="sat4"
                     /locus_tag="ACG8ZM_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFQ0493847.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            9869..10663
                     /locus_tag="ACG8ZM_002992"
                     /note="aph(3')-IIIa"
     CDS             9869..10663
                     /locus_tag="ACG8ZM_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFQ0493848.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            10939..11070
                     /locus_tag="ACG8ZM_002993"
                     /pseudo
     CDS             10939..11070
                     /locus_tag="ACG8ZM_002993"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            11146..11639
                     /locus_tag="ACG8ZM_002994"
                     /pseudo
     CDS             11146..11639
                     /locus_tag="ACG8ZM_002994"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         4178 a         1835 c         2518 g         3265 t
ORIGIN      
        1 tagaaacaaa atcagataag tatttttctt cggaggggga agattatata tataagttaa
       61 tagaaaataa caaaataatt tattcgatta gtggaaaaaa attgacttat aaaggaaaaa
      121 aatctttttc aaaacatgca atattgaaac agttgaatga aaaagcaaac caagttaatt
      181 aaacaaccta ttttatagga tttataggaa aggagaacag ctgaatgaat atcccttttg
      241 ttgtagaaac tgtgcttcat gacggcttgt taaagtacaa atttaaaaat agtaaaattc
      301 gctcaatcac taccaagcca ggtaaaagca aaggggctat ttttgcgtat cgctcaaaat
      361 caagcatgat tggcggtcgt ggtgttgttc tgacttccga ggaagcgatt caagaaaatc
      421 aagatacatt tacacattgg acacccaacg tttatcgtta tggaacgtat gcagacgaaa
      481 accgttcata cacgaaagga cattctgaaa acaatttaag acaaatcaat accttcttta
      541 ttgattttga tattcacacg gcaaaagaaa ctatttcagc aagcgatatt ttaacaaccg
      601 ctattgattt aggttttatg cctactatga ttatcaaatc tgataaaggt tatcaagcat
      661 attttgtttt agaaacgcca gtctatgtga cttcaaaatc agaatttaaa tctgtcaaag
      721 cagccaaaat aatttcgcaa aatatccgag aatattttgg aaagtctttg ccagttgatc
      781 taacgtgtaa tcattttggt attgctcgca taccaagaac ggacaatgta gaattttttg
      841 atcctaatta ccgttattct ttcaaagaat ggcaagattg gtctttcaaa caaacagata
      901 ataagggctt tactcgttca agtctaacgg ttttaagcgg tacagaaggc aaaaaacaag
      961 tagatgaacc ctggtttaat ctcttattgc acgaaacgaa attttcagga gaaaagggtt
     1021 taatagggcg taataacgtc atgtttaccc tctctttagc ctactttagt tcaggctatt
     1081 caatcgaaac gtgcgaatat aatatgtttg agtttaataa tcgattagat caacccttag
     1141 aagaaaaaga agtaatcaaa attgttagaa gtgcctattc agaaaactat caaggggcta
     1201 atagggaata cattaccatt ctttgcaaag cttgggtatc aagtgattta accagtaaag
     1261 atttatttgt ccgtcaaggg tggtttaaat tcaagaaaaa aagaagcgaa cgtcaacgtg
     1321 ttcatttgtc agaatggaaa gaagatttaa tggcttatat tagcgaaaaa agcgatgtat
     1381 acaagcctta tttagtgacg accaaaaaag agattagaga agtgctaggc attcctgaac
     1441 ggacattaga taaattgctg aaggtactga aggcgaatca ggaaattttc tttaagatta
     1501 aaccaggaag aaatggtggc attcaacttg ctagtgttaa atcattgttg ctatcgatca
     1561 ttaaagtaaa aaaagaagaa aaagaaagct atataaaggc gctgacaaat tcttttgact
     1621 tagagcatac attcattcaa gagactttaa acaagctagc agaacgccct aaaacggaca
     1681 cacaactcga tttgtttagc tatgatacag gctgaaaata aaacccgcac tatgccatta
     1741 catttatatc tatgatacgt gtttgttttt tctttgctgt ttagcgaatg attagcagaa
     1801 atatacagag taagatttta attaattatt agggggagaa ggagagagta gcccgaaaac
     1861 ttttagttgg cttggactga acgaagtgag ggaaaggcta ctaaaacgtc gaggggcagt
     1921 gagagcgaag cgaacacttg attttttaat tttctatctt ttataggtca ttagagtata
     1981 cctatttgtc ctaatatgat tttagcagta taattgactt ggtgaatagg tcatttaagt
     2041 tgggcataat aggaggagta aaatgaaaaa atttatttat cgagttttag aaaatgacga
     2101 agtggtggct atttttaatg agcaacaata tgcgcaagat tttatcgctt acgaaaagac
     2161 aatttctgat aagcaatttg aaattgaaaa agtagatatt gctgattggt tattgcaacc
     2221 gagagaattt tagaggttgg ttgaaaatgg ctaaaattgg ttatgcacgt gtcagtagca
     2281 aagaacagaa cttagatcgg caattacaag cgttacaggg cgtttctaag gtcttttcag
     2341 acaaattaag cggtcaatcg gtcgaacgcc cacaattaca agctatgctt aactatattc
     2401 gtgaagggga tattgttatt gttactgaat tagatcgatt aggacgaaat aataaagaat
     2461 taacagaatt gatgaatcaa attcaaatta agggggcaac cctggaagtc ttaaatttac
     2521 cctcaatgaa tggtattgaa gatgaaaatt taaggcgttt gattaatagc cttgtcattg
     2581 aattgtacaa gtatcaagca gaatcagaac gaaaaaaaat taaggaacgt caggcacaag
     2641 gaatcgaaat tgctaagaaa aaaggcaaat tcaaaggtcg tcagcataaa tttaaagaaa
     2701 atgatccacg tttaaagcat gctttcgatt tgtttttgaa tggtttatcc gataaagaag
     2761 ttgaagaaca aactggaatc aatcgccgaa cgtttagaag gtatcgagca agatataacg
     2821 tgacagtcga tcaaagaaaa aacaatgaaa agagggatag ttaatgagta cggttatttt
     2881 agctgaaaaa ccaagccagg cattagccta cgcaagtgct ttaaaacaaa gcaccaaaaa
     2941 agacggttat tttgagatca aagacccact atttacagat gaaacgttta tcacctttgg
     3001 ttttgggcat ttagtggaat tagcagaacc aggtcattat gacgaaaagt ggcaaaattg
     3061 gaaacttgaa tctttgccga tttttcctga tcgatacgat tttgaagttg caaaagataa
     3121 gggaaagcag tttaaaattg ttgctgaact tctcaaaaag gcaaatacaa ttattgtcgc
     3181 aacagatagc gacagagaag gtgaaaatat cgcctggtcg attatccata aagcaaatgc
     3241 cttttctaaa gataaaacgt ataaaagatt atggatcaat agcttagaaa aagatgtgat
     3301 ccgaagcggt tttcaaaatt tgcaaccagg aatgaattac tatccctttt atcaagaagc
     3361 gcaaacacgc caaattgccg attggttgat cggcatgaac gcaagccctt tatatacctt
     3421 gaatttacaa caaaaaggcg tacaaggtac attttcatta ggacgtgtac aaacaccgac
     3481 tttatatctc atttatcaac gccaggaagc aatagaaaac tttaaaaaag aacctttttt
     3541 cctgaataat tcatagtttt actaatagtc tttcctaaca ttgatttaat agtctttaaa
     3601 gtattctgct tcatcaccca ttaggtgatg aaaaaagaac ccctttctga tttagcggac
     3661 aaatactttg acctaatctc agaagatata aaagaaaaaa taataaaata tccgatttat
     3721 actttgtata aagaactaaa gtagttagca aatcatttac agcaaatcac tatgttccac
     3781 ataccatttt acatacttac cttatccacg acacatgttg agatagtatc gaagataaag
     3841 aaagttgctc catgttgttt ttgcgagatg gggaaatcaa agccacattg acaaccatga
     3901 tgaataagct tgctttcagt cataagctaa tcctagaacc acttttcaag tcagttagcc
     3961 aaatcgatga agaaagcgac tgtgaaagaa tggacgctat tgataagcta atggagcaac
     4021 tattggaaga acgcaatacc cttattgcac tgatgtccaa aggtttccta gaacctgctc
     4081 tttttaatca ggaacgaaat gttttagata gtgagattaa aaatcttact accgaaaaaa
     4141 caaacctggt aacgaactct gcgagtgggg ttttgcgagc aaacgagata aaggacctca
     4201 ttaattatgt gtcagcagat aattttaatg gtgactacac ggaagaacta tttgaagaat
     4261 ttgtagtgaa catcattgta aattccaggg atgagctgac attcaatttg aaatgcggtc
     4321 tttccctgaa agaaaaggtg gtgagataaa tggcatatat tccatatgga tacaaaattc
     4381 aagatggagt ggttactgtc gatgaaaagg cagcaggtca agtaaaggta ttctttgaga
     4441 aatacatatc aggactatcc cttacagtgg ctggcgaaca ggcaggtatt gaaaagacac
     4501 attctgtgat ggggcgcatt ttgaaaaacg tcaactacct tggaaatgat acgtatccag
     4561 caattattga taaagagata tttgataaag ctgaagaagt tagagataaa cgtgcaaagg
     4621 atttaggacg agtggtagag cttgccgctt tcacctctcc ccctcccaaa gaacgattca
     4681 aaatgaaaaa ggcagataat aagatgccag tggatccttt tgaacgagca gaatacttat
     4741 acagtctgat agaaagcgag gaataaagtg acagagaaaa atataatggt tattcctgct
     4801 cgtaaaagag taggaagtac agccgcaaaa gaaaagataa agaaacttcg tgttgctgcc
     4861 tattgccgtg tttctacaga aacagaagaa caaaattcta gctatgaggt tcaggtctca
     4921 cactatacag agtttataaa gaaaaataat gaatgggagt ttgctggcat ctttgcagat
     4981 gatggtatct ctggtacgaa cactaaaaag cgtgacgaat ttaatcgcat gattgcagag
     5041 tgtatggacg gtaacatcga catggttatt acaaaatcca tcagccgatt tgcgcgtaac
     5101 accctagact gccttcaata cattagacag ctcaaggata agaacatatc agtttatttt
     5161 gagaaagaga acatcaacac cacggatgcc aaaggtgagg ttttgctgac tattatggca
     5221 tctttggcac agcaggaaag ccagagcctt tcacaaaacg ttaagcttgg gctacagtac
     5281 cgataccaac aaggaaaggt gcaggtcaac cataagcgat ttatgggcta cacgaaagat
     5341 gaagatggaa atttaactat tgtccccgaa gaagctgaga ttatcaaacg catctaccga
     5401 gaataccttg agggtcagag tctagtgggc attggtcgag cccttgaaaa ggatggtatt
     5461 ttaacagcag cgggaaaacc aagatggcga ccagaatcag ttaagaagat acttcaaaac
     5521 gaaaaataca tcggagatgc ccttctgcaa aagactgtca cagtagattt tctgaccaag
     5581 aaacgagtga aaaatgaagg gcatgttccc caatattatg ttgaaaatag ccatgaagcg
     5641 attattccta aagagttatt tttgcaggcg caagaagaaa ttcatcggag aagcaatatc
     5701 tacacaggag aaggcaagaa caaaagaatt tacagtagca agtacgcttt aagtgccatc
     5761 accttctgtg gagattgtgg cgatatttat agacgggtct attggaatat ccacggaaga
     5821 aaagaatttg tctggcgatg cgtgactaga atcgagcaag gtcctgaagt ttgtaagaac
     5881 cgaaccgtaa aagaagatga actttatggt gcagtaatga ctgcgattaa taagctactt
     5941 gcaggtggga acaatatgat aaagacactg gaagagaata ttcatgctgt gattggcgaa
     6001 accacagaat accaaatttc agagattaac aactcactag aggaaaaaca aaaagaacta
     6061 atcaagctgg cgaacaaggg tcaagactat gaccacttag cagatgagat tgatgagctg
     6121 agagacaagc gacagatcct tttagtagaa gatgcctccc tcagtggcga gaacgagcga
     6181 atcaatgagc tgattgaatt tatccgcaag aacaaattcc gcaccttaga atatgatgat
     6241 aagcttgtaa ggaagataat ccagagcgtt acagtctatg aaggccactt cgtcatagcc
     6301 ttcaaatctg gcatcgaaat ggaaatatga aaaccagaag taaatagccc atgactctac
     6361 agtagagttg tgggttttct tttgcttgct gattataata atattttaat aactattgtg
     6421 tttatcaacc aaatggttta taatattctt agcgtaaact tggaggtgct gtatgactac
     6481 ggcagaaatg attaaagaac tgtgtgagca aatgaatata agtgtttccg aacttgctag
     6541 acgtattggc cagactccac agaatttcaa taaaaaatta caacgagaaa cagtaacctt
     6601 ggatgagttg aaggccattg ctgatgtgct aggtgtcaag tttgtgcagg catttatttt
     6661 accagatgga gaagaaataa aaatatctaa cgaataaagg aggatgttta tatggtaggt
     6721 aatgttattc aaatagtaac agagaaatta tcatctttgc cttttataga aggcatcgta
     6781 ttagggggct cacgtgcaag aggcacccat acagaaaatt ctgatataga tatcggaatc
     6841 tattacaatt cagactcatt tgacctgaca gcgattaatc aaattgctac agagttggat
     6901 gatgagaata gaaacaacct tgttgtacct cccggagcgt ggggtgattg ggttaatggc
     6961 ggtggatggt tagttataaa cgggtgtcat gttgacttga tattacgtga tataaaacgg
     7021 gtggaacaaa taatcaaaga tacggagcaa ggaattgtta ctgccaatta tcagaccggg
     7081 catccccatg gttatataag tgctatgtat cgtggagaat tagcgattag taaaatacta
     7141 tatgctaaga acgaaagctt atgcgaatta aaaaagcagg cagaaattta ccctactgct
     7201 ctaaagaaaa gcttgatgaa cttttttata tttgaagctg aattctcttt aatgtttgta
     7261 aaagcaaatg cgggggcaga ggataaatat tatattgcag gccatgtttt tcgtattatt
     7321 tcatgcttaa atcaagtact atttgcatgt aataatgctt attgcattaa cgaaaagaaa
     7381 gctataaaac tgcttgaaac ttttgaatat aaacctgaaa aatatgccga aagagttaat
     7441 catatttttg aagtactcgg tctttctctt tttgaatgct acgacatgac cgagaaactt
     7501 tataaagaag tgaatgaaat tgtatcggag ataaataact ttttaaacga ggagagttca
     7561 gatgaaagaa aacaaatatg atgataatat attttttcaa aaatacagtc aaatgagtcg
     7621 ctcgcagaaa ggactggctg gtgcgggaga atgggagact ttgaaaaaga tgctacctga
     7681 ttttaagggt aagcgtgtgc ttgatttagg atgcggctat ggatggcact gtatatatgc
     7741 gatggaaaac ggtgcttcct ctgtagtagg tgttgatatt tctcataaaa tgctcgaagt
     7801 agcaaaagga aaaacccatt ttccacagat tgaatatgaa tgctgtgcca tagaagatgt
     7861 ggatttccca gaggagagct ttgatgtaat actaagttcg cttgcgtttc attatgtagc
     7921 agactatgag aatttaataa aaaagatata taggatgctg aaggctggtg gcaatttagt
     7981 ttttacagtt gaacatcctg tttttactgc tcatggaaca caagactggt attataacga
     8041 aaaaggagaa atactgcatt tcccggtgga caattattat tatgagggca aacggacagc
     8101 tatgtttttg gaagaaaagg ttacaaaata tcatagaaca ctgaccacat atctaaatac
     8161 actgctttca aatagtttta taataaatca gattgtggag ccacagccgc cagagaacat
     8221 gatggatatt ccggggatgg cggatgaaat gcgacgccca atgatgctga ttgtatcggc
     8281 aaaaaagaag atgtaataat atagaaaaaa taaacgagga gtatgtaaat gagatcagaa
     8341 aaagaaatga tggatttagt actttcttta gcagaacagg atgaacgtat tcgaattgtg
     8401 acccttgagg ggtcacgcgc aaatattaat atacctaaag atgaatttca ggattatgat
     8461 attacatatt ttgtaagtga tatagaaccg tttatatcta atgatgactg gcttaatcaa
     8521 tttgggaata taataatgat gcaaaagccg gaggatatgg aattattccc acctgaagaa
     8581 aagggatttt cctatcttat gctatttgat gattacaata aaattgatct taccttattg
     8641 cccttggaag agttagataa ttacctaaag ggcgataaat taataaaggt tctaattgat
     8701 aaagattgta gaattaaaag ggacatagtt ccgactgata tagattatca tgtaagaaag
     8761 ccaagcgcaa gggagtatga tgattgctgc aatgaatttt ggaatgtaac accttatgtt
     8821 attaaaggat tgtgccgtaa ggaaatttta tttgctattg atcattttaa tcagattgtt
     8881 cgccatgagc tgctgagaat gatatcatgg aaggtcggca tcgaaacagg ctttaaatta
     8941 agtgtaggca agaactataa gtttattgaa aggtatatat ccgaggattt gtgggagaaa
     9001 cttttgtcca cctaccggat ggattcctat gaaaacatat gggaagcatt atttctatgc
     9061 catcaattgt tcagggcggt atccggtgag gtggcggaaa ggcttcatta tgcctatccg
     9121 gagtatgata ggaatataac aaaatatacc agggacatgt ataaaaaata cactggtaaa
     9181 accggctgcc tggatagcac atatgccgct gatatagaag agaggcggga acagtgatta
     9241 cagaaatgaa agcagggcac ctgaaagata tcgataaacc cagcgaacca tttgaggtga
     9301 taggtaagat tataccgagg tatgaaaacg agaattggac ctttacagaa ttactctatg
     9361 aagcgccata tttaaaaagc taccaagacg aagaggatga agaggatgag gaggcagatt
     9421 gccttgaata tattgacaat actgataaga taatatatct ttactaccaa gacgataaat
     9481 gcgtcggaaa agttaaactg cgaaaaaatt ggaaccggta cgcttatata gaagatatcg
     9541 ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag
     9601 aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta
     9661 tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat
     9721 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
     9781 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
     9841 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
     9901 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
     9961 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
    10021 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
    10081 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
    10141 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
    10201 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
    10261 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
    10321 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
    10381 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
    10441 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
    10501 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
    10561 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
    10621 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt
    10681 ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaagt acatccgcaa
    10741 ctgtccatac tctgatgttt tatatctttt ctaaaagttc gctagataga gttctatatt
    10801 agagatgtaa agagttttgg tgaagaggtt tgtgagtatg gcttttataa ccagggtgca
    10861 atgagaagca ctaacagcag ctagcttaag aacgcatagg atatactttt tatggaagtc
    10921 acagaggagg aataaaattt gaacaggatt aacagagtaa cctctattct tattcagctg
    10981 caatcaaaaa aaataattcc tgccaaagaa attgcacagc gatttaatat aagcttaagg
    11041 acagtttaca gagatatccg gacacttgaa gaagccggaa taccaattgg atctgaggcc
    11101 ggaaaaggat attttcttgt agagggcttc ctacttccgc cggtaatgtt tacagcggcg
    11161 gaagtgggtg cattgattac agcagggaaa tttttaaatt gccatggaga tgaatcattt
    11221 ataaaggatt ttgattcagc tatgtataaa atcaaatcta ttttgaagca tggtgaaaaa
    11281 aactatgcac aggagttgga gaacagtatt aacgtgtaca gcacatctgg acagaaaaat
    11341 acattggcgg ataacgttat tgcagcaatt cagactgcaa tctgcaataa aagggtaata
    11401 tcaattcaat atcctgcatc aggagggcaa gaaccggaaa gcagaatgat agagcctata
    11461 tcactggggt tttatgaaca gaactggtac ttaatcggtt ttgcaggcta agaaatgaat
    11521 ataggaattt tcgtgtggat cggattaaaa gcatttgcat agaagaagag ctctgtcaaa
    11581 gccatgatgg ctctttggaa caaatattaa aacaaatgct ttcatataaa aaactatatg
    11641 ccctacaatg ccgttgtaaa aattggggaa aagggatata ttgattttat atctttctca
    11701 aaataaatat aaaagccctt taaagagggc ttttatatat taatcacaaa tcacttatca
    11761 caaatcacaa gtgattaatc acaaatcact tgtgat
//
LOCUS       DAZKOR010000265         7561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.guided.108, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000265 DAZKOR010000000
VERSION     DAZKOR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.guided.108"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..485
                     /locus_tag="ACG8ZM_002995"
     CDS             <1..485
                     /locus_tag="ACG8ZM_002995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493849.1"
                     /translation="SKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAV
                     FNSNQSVLLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHE
                     IAEVIMVLSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK
                     "
     gene            592..1830
                     /gene="mef(H)"
                     /locus_tag="ACG8ZM_002996"
                     /gene_synonym="mefH"
     CDS             592..1830
                     /gene="mef(H)"
                     /locus_tag="ACG8ZM_002996"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFQ0493850.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            2057..2476
                     /locus_tag="ACG8ZM_002997"
     CDS             2057..2476
                     /locus_tag="ACG8ZM_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0493851.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            2481..2720
                     /locus_tag="ACG8ZM_002998"
     CDS             2481..2720
                     /locus_tag="ACG8ZM_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0493852.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(2945..3868)
                     /locus_tag="ACG8ZM_002999"
     CDS             complement(2945..3868)
                     /locus_tag="ACG8ZM_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0493853.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(3900..4664)
                     /gene="map"
                     /locus_tag="ACG8ZM_003000"
     CDS             complement(3900..4664)
                     /gene="map"
                     /locus_tag="ACG8ZM_003000"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0493854.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4822..5262
                     /locus_tag="ACG8ZM_003001"
     CDS             4822..5262
                     /locus_tag="ACG8ZM_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0493855.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(5440..6009)
                     /locus_tag="ACG8ZM_003002"
     CDS             complement(5440..6009)
                     /locus_tag="ACG8ZM_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0493856.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            6263..7495
                     /locus_tag="ACG8ZM_003003"
     CDS             6263..7495
                     /locus_tag="ACG8ZM_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0493857.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         2419 a         1312 c         1401 g         2429 t
ORIGIN      
        1 attcaaagga agatatttta aaagcaattt ttcaaagaat tggaaatgaa aatgcagtaa
       61 tatttgctaa aattagagat aacgatagtt taaacggttt agaaaagtta agagaaatat
      121 ttaagacagc ggtattcaat tctaaccaaa gcgttttatt gacagtttct cctcaactat
      181 taaacaatcc tcgttttctt gctatgcaaa tagaacaaat gtatgaaatt gtagtacctg
      241 aatttgttga acccgtttta gaacaaggta tcaaggataa gtctttggag attgaaaatt
      301 cacatgaaat tgcagaagtt atcatggtat taagtaatgt atggctcaac ccattagttg
      361 aaatgacgga taaagagggt atggaaaaac gttgcaatac attcaatgcc cttttaaatg
      421 gaataggcat agacacatta ttagacgaag aaacaatttc tgcatttata cagttttgta
      481 aataactgta tttgttatcc ctatacgata gactgctcta taaaagagca gtttatttaa
      541 accattatat aaaccgacgg tctgtttgat taaataaaaa ggagagattt tatgcaagca
      601 gaaaaaaaac tttttaatcg tgattttata cttgttgtta taggacagat aatttcttta
      661 tttggaaacg ctatcctacg atttgcatta ccgtactatt tgttaaaaga aactggttca
      721 tcagctttgt ttggtattgc tacagcctgc tcttttttac ctatggttat tttgtcgctg
      781 gtaggtggta ttattgctga caggacaaat aaaagaaaca ttatggtcat actagatttt
      841 gtaacagcaa tcataatttt agtattttat ttgtcattgg gaaaaattcc tatcatacct
      901 ttgtttattg ttgctttgat gttactttat ggtatttctg gtgcatatca accagcggta
      961 caagcaagta ttcctctatt ggtatcatca gataaactta ccgtcggtaa tgctataata
     1021 aatcaagtaa atactttagc aaactttatc ggaccagcta taagcggagt agtttttcaa
     1081 ttttatggta tcaatcccat tttaattgtt agtattatat gttttacttt ttcagccatt
     1141 atggaaattt ttatccatat tccacaccaa aaaagacctc gtgaagtagg tgtaattgca
     1201 gttgctaaaa acgatttaaa agatagttat acctttttga aaaaggagaa acccattttt
     1261 atatccgtaa tctttttggt ttgcattttc aatttggttt taagtgctgt tttaattgtt
     1321 ggtctgccta tcttaatttc tgaagtttta aaaatgccag atacactata tggtttttca
     1381 caatcagcat tatcattagg tggtttatgt ggtggtattt taacagctct tgttgcttca
     1441 aagttaaaac taaaaaacct ttataccttg ttacttattt gttctgtttc tgttttactt
     1501 atgggaattt cgctattttt agaattgcct gcaatagttt cctactgggt agttacagtg
     1561 atgtgctttg tggctatggg tgtatctact atgtttgttg ttcaaattta cactcttgtt
     1621 cagacacaaa caccaccgca attagtagga aagataatgg ctacgctggt agcagttgct
     1681 atgtgcggtc aacctattgg acaaatggtt tacggtattt tattcgatgt atttatacaa
     1741 gaaacatggg ttgttatgtt tgtagcttct attgcttcaa tcatgatttc aatatattca
     1801 aaaagaattt ttcaaaagtt agagatataa tacaaggggt taaatagtat gttaggctaa
     1861 aaacaatata taaaagaaaa tttgaaatct gctaaaagga tagtgttata aaagaaagag
     1921 taatattgca gtatttaagg ctcgcacaaa tatatgtttt gtgtgggctt ttttcttacc
     1981 atttttctgt atttctaaag ggtataaacg gtattccgtt tatatatttc agcattagca
     2041 gaaagggggt gagattatga ggacatcttc tttcgagcat atcgtcagat tacagttcaa
     2101 ttctttaatg ctgattgtca ttaagaacaa agtgaaaagt cgaaacagac agcttgcaag
     2161 acattctaaa cgtgaagtat tgttttgtga aatagccgaa acgaaacaag ttgaacgtgg
     2221 aacgattgat acctgttttt atgattgtgt atcatttaaa gtcttgcact ttacaattta
     2281 tgtatctgat gaaacacttg gtacagtttt acatagactg tcagaaaaac agcgtagtgc
     2341 gattttatta cgttattttc aaggaatgaa cgacagagaa atttcagaac tgtaccatgt
     2401 atcacgttct gctatttgca gtagaagaac tagaggacta aggaagttaa aaatgctttt
     2461 gagcgaaagg aagtaaagaa atgagtaaag attatggcta tccctctttt gaattgattt
     2521 gcaaagcgtc aagcggagat gaaatggcag ttaaagagat tttgaagttt tatgacggtt
     2581 atatttctaa attatgcttg cgtccttttt accactctga aagtggtaaa atcattatgc
     2641 aggttgatga agaactaaaa ggagaaattc atacagacat gatgaaagca atattgaaat
     2701 ttgaaatcgg agtgaagtaa tcaactgtat aaaaaaatcg ggagatacac agtttcgtgc
     2761 tatctccctt agtagtttta cggtcatatc aaggctcatt caatcgtggt aaattcgtgg
     2821 taagatgagt gtttttctat acttcaaaat gcttgaatgt atagtgttta tgcggataat
     2881 tgaaaattag atataaaatt tcaaataaat tactgatata aatttacaat aagaactctt
     2941 gtttctactt ttttttatga gaaaatttag ttttcaattt attgaaccat tttcggcttg
     3001 gatctcgacg ttttttgatt tgatgacccg tcacgtattc ttgaacaact gcattgatga
     3061 ttccgcccaa aatgatgatc gtagcagcaa aattaagcca aagcatgagt acaataaaac
     3121 ttcctatgat ctgataaccg ctgatcctcg ttgcaaaata cctagcatag atactaaaca
     3181 cttgtgataa cagcatccag ccgacagtag cgaagatcgt gcccggaatg atcgtcttca
     3241 acttcaactg agcattaggg acaattaaat aaatcaagaa catgatgaca aataaagcta
     3301 ccaatgtgat tggccacttc aaagtttgga actgatcgat aaagttcacg ggtatttgta
     3361 cgattggctg aatagcatcc aaaatcagct gtcccaaccc aagaacgata gttaccccac
     3421 taatagcaat catgaacaag acaatgacaa ttagtgagaa gaaacggacg atcaaaaaat
     3481 tttttctatt ttctacgcca tacgctttat tcatcgcaat ttgcaaagca ttgatacttt
     3541 gacttgcaga ccataaagcc gctaatgcag aaacggataa caagcctcct gaactttgtg
     3601 tcaaaaggtc tttgattgca ggaccgataa actggtaaac ggtctcagga atcacttctc
     3661 gtaaataggt cagtacagtc tcttgatcga tgtggagaaa cggtaaaata ttaccaaaag
     3721 caataatcaa tggaaataaa gacaagagta aataataagc aacgacgaca gaggtcgtac
     3781 tcatttctga atcagtgatg cgcttttgag tcgtctcaat aaagcgcatc agattttcat
     3841 tttgttttat tttgttccaa actttcatgt acctctcccg tacctattga ctggatagat
     3901 caataagtgc cttcttctcc ttgtgatgtc aaaattcttg gaccatcttt agtaatagcc
     3961 aatgtatgtt catattggca agaaagtccg ccgtctcttg tatacgcagt ccagccgtta
     4021 ggatccattt tcattttcca agttccggta ttaaccatag gttcgatcgt gataaccatg
     4081 ccttctttca accgcaaccc ttttccagct tctccgtaat gaggaactgc tggttcttca
     4141 tgaatcgtag gaccaacgcc atgaccaata aaatcacgaa cgatcgctag attttcgcct
     4201 tctacataag tttggatagc atgaccgatg tcaccaatcc gattccctac ttgtgcttgc
     4261 tcaataccga tgtacaaagc tttcttcgtt acttccatca atcggtcgat ctcaggtgta
     4321 gattctccaa ctacataggc ccaacaagaa tcagaaatgc cgcctttcaa atcgatacac
     4381 atatcgactt ttaccaaatc accgtttttc aaaggctttt tacgagggaa tccatgacaa
     4441 atttcatcat ttatactgat acaagtcgca tattcatacc cttcaaagcc aatttgcgca
     4501 gcaattccgc catgactctc gatatagttt ctgacaaata cctcaatatc ccaacttgtg
     4561 attcctggtt ttataaaatc acgcagattt ttatgtacat ctgctaataa agctccagat
     4621 tctgccatca tttcaatttc acgtgctgat tttaaagtaa tcatattttc gtctcctttt
     4681 tacaaatccc tctcatttta acatagcggc atacatttag ccaaatgaca ttccccaggt
     4741 gtctctaggt tttattttct tttcaatgat gttttggtat aattcataat tgttaaataa
     4801 ttataaacga ggaggcacat catggcttta gcaaaaatcg tatatgctag catgacagga
     4861 aacactgaag aaatcgccga tattgttgca gaagcatttg aaaatctgga tatcgaagtc
     4921 gaaatcaacg aatgtaccca agtagatgca gatgaattcg aggaggcaga tatttgcgta
     4981 gtagctactt atacatacga tgatggcgat ctgcctgatg agatcgtaga cttttatgaa
     5041 gacttgcaag aattggatct attgggaaaa atttacggtg tctgcggatc tggtgatact
     5101 ttttacgatg aattctgcaa atcggtggat gatttcgatg ctgcttttgc aaaaacaggc
     5161 gcatcaaaag gagccgaaaa tgttaaagtg gatctgaatg ctgaagaaga agatatcgaa
     5221 aatttagaag ctttcgctaa agaactagtc gcaaaaattt aatatacttg caataaaaga
     5281 gccatccaga tttattcaat ctgtatggct cttttattca ttgcattcat ggaagagttc
     5341 ttcgggagtt aaaaatgcat acgctgtaag aaaaacgaac ttttccttat tttgacacat
     5401 ctcttgttac ctactgattt gtggaacttt tgagtatatt caatcgattt cgattggttc
     5461 tttgaatttc aagatagcat ctatactgtc ttggatcatc ttttcaatct gttcatcagt
     5521 tggatggatg atgcttgaag acatcaaagc tgcgatccca gtagctatga tccatgtccc
     5581 attatacaaa gattcgatct gctcttctga aagattcaca taagctggat cgcgtttcac
     5641 tgtattcaca aagtaagtat aagagaaatt ctgcatctct tttcctccac catattcttc
     5701 taagtacagt gaacgataca acttcttctc tcttcttgca aaatggatat aattaaaagc
     5761 aagatcgata atcgtattcc ctgtatgctc aacagtgaac acttcatatt gtaaatgatc
     5821 atagatctgc tcaaaaagag cttgtctcaa gtcatccata tttttaaact caagataaat
     5881 cggttgcgtg gaacaattca ttttgttcgc gatgtttctc gcagtaaatc gtgtaaatcc
     5941 atcggtggca gctacttcat aagcagctgc caaaatctgt tctctagtaa tcgtcttttt
     6001 tcttgccata ttttttaatc tccttaattt ttttataacg cttccaccat tgattaattt
     6061 cattgtaaca tgtttttttg atatgcaaaa ttagtttttg attttcttca caagctgaaa
     6121 tagcaagtag taacatatat catgaaactt aaccttaaaa aaataacctc ctttttttat
     6181 ttaaagcctc attgtttgaa tcataattaa atggtatcat gctaatagtt accagaaaaa
     6241 atggtgagga ggaattttta ttatgttatc tgattttaat gaacttctaa aaaaatatgc
     6301 acgcttaatt gcagaaactg gcgtagccac agaaaaagga cacacagttg ttttacagat
     6361 cagcgtggaa caggcacccc ttgctcgatt gatcacacaa gaagcttata aactaggagc
     6421 cgctgaggtt atcgttcaat ggactgacga ccagatccaa agagaatttc ttttacatgc
     6481 tgcaacggat cgtattgaga atgtaccaca atctaaaatc gatcaagcgg atgaatggct
     6541 tgaaaaagga gcaagcagga tcagcgttgt ttctgccgat cccgatgcat ttgctggtgt
     6601 agatagccat cgagtagcgt cttatcaatc agcgaatggc aaagcactaa tgaatttacg
     6661 caaggcaact caagcaaata aagtcagttg gactgtcgtg gctgcggctg gtaaacaatg
     6721 ggcagcaaaa gtcttcccag aactgccaga agaaaaacaa gttgaggcat tatgggatca
     6781 gattttcaaa acgactcgtg tctatgaaga agaccctgtc ttagcatgga agaagcatga
     6841 tgaaaaatta gcaaaaaaag cagaagaatt gaatcgagaa caattttctg cccttcacta
     6901 tactgctccc ggtacagata tcattatcgg attgcctaaa aaccatcttt gggaaggcgc
     6961 tggaagctat aatgctcgtg gagaaaaatt catggcaaat atgccgacgg aagaagtgtt
     7021 cacagcacct gacagtcatc gagtagatgg ttacatttca agtacaaagc cactgagtta
     7081 tgctggcacc atcatctcgg gtatgaaatt tacttttaaa gatggaaaag ttgttgattt
     7141 ttctgccgaa caaggagaag atgtcttagc aaaactgctt gatacagatg aaggtgcgcg
     7201 tcgtttagga gaagtcgcac ttgtacctga cccttcacct atttctcaat ctggcatcat
     7261 ctttttcaat acattatttg atgaaaacgc atcgaatcac ttagcactag gttctgctta
     7321 tgccttcagt gtgaaaggcg gtacagaaat gtccgatgaa gaattagcag aagctggatt
     7381 gaatagaagt caaacccacg tagatttcat ggtaggatct gacaagatgg atatcgacgg
     7441 aatccgtgaa gatggctcta cagtaccgat tttccgaaat ggcgattggg cttgagtata
     7501 ctttcgcaca catgcaaaaa ataatggctc tttgtcaata aggactgatg agttgtgcaa
     7561 a
//
LOCUS       DAZKOR010000266         5280 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.guided.131, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000266 DAZKOR010000000
VERSION     DAZKOR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.guided.131"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..519
                     /locus_tag="ACG8ZM_003004"
     CDS             <1..519
                     /locus_tag="ACG8ZM_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0493858.1"
                     /translation="NFSSSEPKTDLFFGATNNGRKERAESYKQEFIKRDVTTNYFILP
                     HRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQKKLVTDNQAIA
                     HYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKRFITNDSTIYE
                     QYEYDLSSVENK"
     gene            complement(669..1610)
                     /locus_tag="ACG8ZM_003005"
     CDS             complement(669..1610)
                     /locus_tag="ACG8ZM_003005"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0493859.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1736..2857
                     /locus_tag="ACG8ZM_003006"
     CDS             1736..2857
                     /locus_tag="ACG8ZM_003006"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0493860.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3115..4617
                     /gene="eat(A)"
                     /locus_tag="ACG8ZM_003007"
     CDS             3115..4617
                     /gene="eat(A)"
                     /locus_tag="ACG8ZM_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0493861.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4684..5211
                     /locus_tag="ACG8ZM_003008"
     CDS             4684..5211
                     /locus_tag="ACG8ZM_003008"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0493862.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1721 a          947 c         1122 g         1490 t
ORIGIN      
        1 aatttttctt ctagcgaacc aaagacggat ctattttttg gtgcaacgaa taatgggaga
       61 aaagaacgag cagaaagcta taagcaggaa tttataaaaa gagatgttac gaccaactat
      121 ttcattcttc cacatcgagg gaatgaacaa cctggatatc ttacttatga agagtacctg
      181 aaaaaaacag gggaatcgcg cgggattctg gaactgttgc gggagggtca gcaaggagtg
      241 actttgcgga catttgaatc catgtatttt caaaagaaac tagtgacgga taatcaagcg
      301 attgctcatt atcattttta tcgtcctgaa aatatttttt tgcttcgaga acgtgattta
      361 gatgaattac cggaatttct gcatacacca tttaagccaa tcctatcaga gctattggaa
      421 ttttatgatg cagaaaattg ggcaaaacga tttatcacta acgattccac tatttatgag
      481 caatacgaat atgacctctc ctctgttgaa aacaaataaa aaacctacat tttgaagaga
      541 ttatgtactg aaacaaacaa gcacaattct tatggcatca tgagagttgt gttttctttt
      601 tgatctttta ttgggcttaa aaaaagagaa ccagctgctg attcagctga ttctcatcag
      661 agagcctttt attctgcgcc aaggattcct tcttttgcat ggactaattg ggtcaaaaag
      721 gcacaatatg gtgtagcaat atcatatttt tgtccttttc gccaaatggc accattgatg
      781 tagtcgattt cagtcaaacg atggtttttg atcaggtctt ggtacataga tgggtaatgc
      841 aacccgatgt tgtcaggatc ataagtagaa gcaatatgtt ggtacacttc ttcttgatct
      901 agtatgattc cttcttttgc tgcgatagaa gcaaattcag atacgattgt tcgaaccatg
      961 ctctctgccg ctttttgcgc gcctagttca gcaatattac aatccaaaat cgtgcataaa
     1021 ccattcaatg ttccatttac acaagctttt cgccaaatgg aataacgaac gttatggcta
     1081 tagatcggat ttaaatttgc ttcttgaaag acttctacaa ctttttttgt aaatgcttcg
     1141 ccttctggtt ccaagttctc tagttcgatt tctccatcac ctaacaatgt gacttttccg
     1201 ggaccagcta gaccagctgt ccacatcgtg atcccaaata agatattttt ctttggcaca
     1261 tatttttcca gtacatcttc atgacctaat ccatttagta aacacaaaac ataagtattg
     1321 tcagtaataa tggattggat cgaacaaaac atatcatcta attgattggc ttttgttaat
     1381 gctacgatca agtccacgtg ttcgttgctt tcgatgattt cttctggtga atagattggc
     1441 agttttgcga ccacttcttt accgttaaag tcagcaatca acccatttgt acgaatggct
     1501 tcaatgtgag caggccaacg atcgatcaag aggacctcat ttccgctttg atgtagcatc
     1561 aatccgattc ggctgcccat tgctccggcc ccagctattg cgattttcat gttttttctc
     1621 tcctttattc aatgagttcc cttttagtat atagaaaaat ggaagaaaat agcattgttt
     1681 tgtttgaaaa aaatgaaaaa aagtgtctta cttaagaaga aagtgaggga tacagatgga
     1741 tattttggcg gcaattgatt catttaaagg aagtgcttct tcatttgaac taaatcaggc
     1801 agttctaaat ggctgggaag atcgtaaagg aagaaaaatc aatgtgccga ttgcagatgg
     1861 cggagaaaat actgcagaag caatctatta cgcgctcagg ggaacatggc gtaccattga
     1921 tgggatggat ctcttattcc gtcagcgaaa aatcagctat ttattgacaa cttggcaagg
     1981 aaaaaacacc gcagtcatcg aatctgcaca agcattgggg ttagatctac ttgataaacc
     2041 aacggatcaa actatccgac ttgcttccag ttatgcctta ggagaactta ttaaagatgc
     2101 actgcttcaa gaagtagaac aaattattgt cactttagga ggaagcgggt gtagcgatgg
     2161 tggtctagga ttactccaat ccttgggtgc ttctttagaa gggtacacag aaggaaatcc
     2221 tttattatct actaaaaaac ttcattttga tcgaagaaac gttcttgttt ctaaagtaaa
     2281 attgattatc gctgcagatg ttaccaatcc atatacaggt caacaaggag ctgcttggca
     2341 atttggaaga caaaaaggag gaatagatac gacgctagct ttcttagata aacaagcagg
     2401 aaagattgct cagcaaataa aagatttctg ggaggtggat ttagatgata ttccaggaag
     2461 tggagcagca ggcggtattg gtggagccct ttttttgttg ggagcgaaga tggtttctgg
     2521 tttcgattta gtcagccaaa tcactggatt ggaaaaaaag atcgaacaag cagatgtcat
     2581 cattacagga gaagggcgga tcgatcagca aacgatccac ggaaaagttc catacggtgt
     2641 agcagtgctt gcaaaaaaat atcgaaaacc aataatcgct atttgcggaa gtagagacca
     2701 agagctagaa ggactttctg attttctccc aattgtctta agtattcatt tgggtccaat
     2761 aggtttagaa gaagcaatgg aacacaggca aacattagaa aaaacaaaaa tcttaggaca
     2821 gacacttagc agatttttca ccatcctccc gaagtagttg acaaaatcat tttctttatg
     2881 ctatcgtaat ttcaaatata ggacaaagtg agggatccgt gcattgaaaa attaagtcga
     2941 ttgatgaaag ctgcattcag actgtacctt gtggagacaa ggaagcagtg tgcagatttt
     3001 cgggattggc ttggtttttt cgatgtatgg gtctttttgt gtgtatccaa cccttgcatc
     3061 ctctttagcc agttctgaaa ataggactgg tcttttttta cgaggtgatg gaaaatgtct
     3121 aaaatcgaaa taaaaaatct gacattcggc tacgacagcc aaggcacatt attatttgaa
     3181 caagcaaatc taaattttga cacacaatgg aaactaggac ttatcggacg aaacggtcga
     3241 ggaaagacaa ctttactgaa tattctacaa aacaaactac cttatcaagg gcaagtaatc
     3301 catcagcaag aatttgccta tttcccgcaa cagacaaaag ataaagaacg tttaacctat
     3361 tacgtgttaa atgatattac ggattttgag atatgggaaa tcgaaagaga gctccaattg
     3421 atgcaaacag atcctgaaat cttatggaga gaattcagca cactatcggg gggagagaag
     3481 acaaaagtcc tactggcact tttatttgtg gatgacactc atttcccgtt aatcgatgaa
     3541 ccaacgaatc atttggatat ctctggtaga aaacaagtag cggcttattt gaaaaagaaa
     3601 aaacaaggct tcatcgtggt cagccatgac cggggattta tcgatgaagt agtggaccat
     3661 gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag ggaatttctc tatctatgaa
     3721 gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa atgaaaaatt gaaaaaagaa
     3781 gtcagtaggc taaagaaaac agcagctgaa aaagccgaat ggtctcgttc ccgagaagga
     3841 gataaaacaa agaaacaagt cggattcatc gatactgaat ctagacgagt gaataaagga
     3901 gcagtgggtg ctgatgctgc acggacgatg aaacgatcca aagcaatcgt gaatcggatg
     3961 gagacccaga tcagcgagaa agaaaaacta ttaaaagata tcgaatatat cgattctttg
     4021 acgatgaata gccaagcgtc tcaccataag cgacttttaa gcgtagaaga tcttcaatta
     4081 gggtatgaaa atctgttatt cgagccaatt cattttacaa tcgagcctca tcagcgggtg
     4141 gcgatttcag gtcctaacgg tgcaggaaag tcatccatta tccattatct tctgggggca
     4201 ttcaacggca aggttatagg agaaaaatac cagccaaaac atctgagcat tagttatgca
     4261 agccaaaatt atgaagacaa tcgaggaacg ttggcggaat ttgcagagaa aaaccaagta
     4321 gactaccaag catttttgaa caacctccga aagcttggga tggaaagaga tgtttttcat
     4381 aacaagatcg agcagatgag tatgggccaa cggaaaaaag tggaattggc taaatcttta
     4441 tcacagccag ctgaactata tacatgggat gaaccattga attatttgga tgtcttcaat
     4501 caagaacaat tagaacaact gatcttgaac gtgaaacctg ccatgttact agtggaacat
     4561 gatcaaacct tcctggataa agtatctact gagattattt ctcttgagag aatctaaccc
     4621 ttgtaatctc aagaacaaca aggtatggtt aaggggaact ttgccaacga aggagagaat
     4681 cacatgcttt atgtaacgag acacggagaa acaacttgga acgcacaagg attagtttgc
     4741 ggacgggcag atgtgccgct gactgaaaaa ggacagatgc aggcacagaa actagcagaa
     4801 aaggtagtag atttgcctgt tccaatcacg aaaatcatcc attcccctct tcaacgagca
     4861 agagatacag cacaagcagt tgctgatcgt ctcagcttgc cgctgaccgt tgatgaacga
     4921 ttggtagaaa tggattttgg ggattatgat ggactgccaa gcaaagatga gaactttcaa
     4981 aaagcacgtt tagcgttcgc tgtacgattc ccaaatgggg agtcagtctt agatgtgtat
     5041 gcacggatcg ttcctttatt gaaagaatgc atcgaagacg aagaaaatgt ctatctgtta
     5101 gtttgccata atgccttgat aagagtgatc aatgcttatt ttcatccgat gcctaatgag
     5161 gggttcttta cattcatggt agataataca gaattggttt cttatgagta attatgtttt
     5221 tgtgttattt aaataaaata tttcaaggca ctgcttttgc ttagcagtgc cttttttagg
//
LOCUS       DAZKOR010000267         4803 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.guided.141, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000267 DAZKOR010000000
VERSION     DAZKOR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.guided.141"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..787
                     /gene="vanR-A"
                     /locus_tag="ACG8ZM_003009"
     CDS             92..787
                     /gene="vanR-A"
                     /locus_tag="ACG8ZM_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0493863.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            765..1919
                     /locus_tag="ACG8ZM_003010"
     CDS             765..1919
                     /locus_tag="ACG8ZM_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0493864.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2134..3102
                     /locus_tag="ACG8ZM_003011"
     CDS             2134..3102
                     /locus_tag="ACG8ZM_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0493865.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3095..4126
                     /gene="vanA"
                     /locus_tag="ACG8ZM_003012"
     CDS             3095..4126
                     /gene="vanA"
                     /locus_tag="ACG8ZM_003012"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0493866.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4132..4740
                     /locus_tag="ACG8ZM_003013"
     CDS             4132..4740
                     /locus_tag="ACG8ZM_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0493867.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          922 c         1119 g         1280 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc ctataatctg ataaattatt
       61 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      121 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      181 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      241 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      301 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      361 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      421 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      481 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      541 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      601 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      661 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      721 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      781 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
      841 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
      901 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
      961 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1021 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1081 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1141 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1201 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1261 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1321 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1381 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1441 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     1501 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     1561 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     1621 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     1681 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     1741 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     1801 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     1861 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     1921 agatgtatat aattttttag gaaaatctca aggttatctt tactttttct taggaaatta
     1981 acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga
     2041 gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt
     2101 tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat
     2161 ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg
     2221 gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa
     2281 tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga
     2341 gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct
     2401 gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat
     2461 tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg
     2521 gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt
     2581 ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga
     2641 tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat
     2701 gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac
     2761 tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact
     2821 gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg
     2881 ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc
     2941 caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc
     3001 acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt
     3061 aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact
     3121 gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc
     3181 taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg
     3241 gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact
     3301 ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa
     3361 ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca
     3421 aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat
     3481 ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc
     3541 cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt
     3601 tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga
     3661 attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc
     3721 tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagttgttgg
     3781 cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc
     3841 ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg
     3901 aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc
     3961 ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct
     4021 gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact
     4081 tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata
     4141 ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact
     4201 tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca
     4261 tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga
     4321 ttgcttctat gggacggtta ccgtcctaag cgtgctgtaa actgttttat gcaatgggct
     4381 gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag
     4441 atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat
     4501 cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt
     4561 atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc
     4621 agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg
     4681 cactatgtat taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa
     4741 acttttaacc gttgcacgga caaacggttc tgttgcaaag ttttaaataa agaataaaat
     4801 ccc
//
LOCUS       DAZKOR010000268         4640 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.guided.144, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000268 DAZKOR010000000
VERSION     DAZKOR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.guided.144"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..198
                     /locus_tag="ACG8ZM_003014"
     CDS             <1..198
                     /locus_tag="ACG8ZM_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0493868.1"
                     /translation="QQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKA
                     KDEVEDTADKSQQIVEEHLRK"
     gene            525..1022
                     /gene="dfrG"
                     /locus_tag="ACG8ZM_003015"
     CDS             525..1022
                     /gene="dfrG"
                     /locus_tag="ACG8ZM_003015"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0493869.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1094..3046)
                     /locus_tag="ACG8ZM_003016"
     CDS             complement(1094..3046)
                     /locus_tag="ACG8ZM_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0493870.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3053..3289)
                     /locus_tag="ACG8ZM_003017"
     CDS             complement(3053..3289)
                     /locus_tag="ACG8ZM_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493871.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3691..3981
                     /locus_tag="ACG8ZM_003018"
     CDS             3691..3981
                     /locus_tag="ACG8ZM_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0493872.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(4253..4602)
                     /locus_tag="ACG8ZM_003019"
                     /pseudo
     CDS             complement(4253..4602)
                     /locus_tag="ACG8ZM_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         1514 a          773 c          799 g         1554 t
ORIGIN      
        1 caacaaatcg caacactgac acaagaaaaa acaaatgtag aaactcagtt aacagataca
       61 caaaagaaat tagacgaagc aactgctgaa aactcaagct tgaaacaata tattcaaaag
      121 ttagaaaaag caaaagatga agtggaagat acagctgata aatctcagca aattgttgaa
      181 gaacatttgc gtaaataatc tttcttaaca agtaaaccta aattagtaaa gaagcaacag
      241 taatctatac taagtagttc agttttggag tacaaaatga caaaatgtac ttcaaaatgg
      301 actacttttt tcattttaac ttacatacca aattacatac aaaatgattt ataaaatgca
      361 attgaaaatg ataattgaat gaaaatttgt gattttgtgg tattatgata atataactta
      421 tagaaaaaat tgcttattca actaacgggc aggataatga gaaaaggttt tttgatagaa
      481 ataagaaatt agatggtaaa ttagttaaaa aggctgtgtt ataaatgaaa gtttctttga
      541 ttgctgcgat ggataagaat agagtgattg gcaaagagaa tgacattcct tggaggattc
      601 ccaaggactg ggaatatgtt aaaaatacta caaagggaca tccgataata ttaggtagga
      661 agaaccttga atcaatcgga agagccttac ctgacagaag aaatattatt ctgacgagag
      721 ataaggggtt tacctttaat ggttgtgaaa ttgttcattc aatagaagat gtttttgagt
      781 tatgtaaaaa cgaagaagaa atttttattt tcggaggaga acagatttat aatttgtttt
      841 tcccttatgt tgagaaaatg tacatcacaa aaatacatca tgaattcgaa ggagatactt
      901 tttttccaga agtgaattat gaggaatgga atgaggtatt tgcccaaaaa gggataaaga
      961 atgataaaaa tccgtataac tactattttc atgtatatga aagaaaaaac ttattgagtt
     1021 aacgggtgcg ttaattgaag aagaacagta aagataaagc acaagagctt gggattactc
     1081 caagctcttt ttgtcatttg aaataaatta tatctttcca atcaaattgt ttatctgtaa
     1141 tccctaatct ttgagcaggt gtcaaaacct ttttatccgc tgatttaaaa gggcgacaaa
     1201 agttgtagta agttcttaga attgtaatcg caaattgagc atacttcgga ttgaagttag
     1261 cgtaaatata gctcttctta tcaccacgag ctgttgttaa tggtctttca agagatgata
     1321 tataacgtct tatctgttga ataaagctat tggtcgaatg gtcattaaca tttaatagca
     1381 tctttgcgat ttcgtttggt tccagagcag ataaatccgt tctacaatca actgaataaa
     1441 atcctttatc cttagtggct aatggatgtt tgatagggtt atcggcatat ttgcgatatc
     1501 tttctccatc tttactaata gcctcttcgt ggaattgatg acttgttagt aattcagtta
     1561 gataatgaga tgccaaagtc cgtagaaact ttgttttgaa tcctctgata tctccccaat
     1621 cgagcagttc tgcttttgct tgtccgaatt cttttaagca ttgttttctg cttttcgtct
     1681 tgttaacttg gcaaataaaa tgatgtgcat cgctcaacct taattctttc gtgaatactc
     1741 tataaaaggc gtttctaata gagaagtcat catcactaat cattcgccat tcgtcggcat
     1801 taaccatttg tttaattaac caatagtgag cagttgtagt ataaggagca tcaacattta
     1861 aaccatcgac aaatctactc ctattagtaa ttttccctaa ttcgtgaaga taagcattcc
     1921 tattttcgct atcgttagcg gatggttctt gtggataata agaccaatct aacctatcat
     1981 ttttacgact aaaagtattg agatggtctt ctttgaattt tcttgtatct tcatttaatt
     2041 catccattga aatattccaa tcataggcaa catcagaacg gaacacatat cttgataaga
     2101 cctcagcaga tacaactata tacgtttgca tattaaggtc ttcaaacccc gtatatttct
     2161 ttgaaccttg tcctttctta cggacgttat ttaggtagta gtgcatcttg tccgtattaa
     2221 gccacatctc gttaaatttc tttgtttgaa ggggttgtgt ctcatatcgt tctaaaaact
     2281 ccaaacaacg cctatacagc cactccagct tatgatagta ggttccaact ccaattccta
     2341 aaatatcaca agtacgactg actgaaaccc ttccaacaac catcttagcg aacattggaa
     2401 gaatggtgtt cttttgttgg tgataagtag tggtttcttc tctttttggt agaacattag
     2461 tgaacttctt acacgcctta cattgatagc gttgagattt gcccctactt ttcccccttt
     2521 tgtaaaattg cttcggctca ttgaatgggg ttgattcttc ttccgagcag ccttctttat
     2581 gaaattgata atctggctca acatcttgga tgctattgat ttctataagg cgtttaattt
     2641 cttcaacaac agaccaatta gataaagggg ttactgtaca gttttgcgat acacctctgc
     2701 caataggatt gtcattgcag tacatacctt tatgaccttt atctttggaa ctaccagtca
     2761 ttttatatcg acttggtttc ccttttacat ctttgaattt gtgctgttct ttcccaaaat
     2821 tacaacaaaa cggattgatg cagtaattat attgaatggg atattcagaa ccgttccatt
     2881 taaaagacgt tggcaagaac aacattttgt tataccttgt tgcaaatcgt tttggagtta
     2941 ataaatcata ttgtttagcc ctatctttta attcttcgtt tgaaatcgga attttaacat
     3001 ctacaatttc atcttccttc gtggctaatc ttttaagctt ggtcattgtc tatcaaccca
     3061 ctctatcttt gatacccatt ttttcaacaa tccgcttatt acttcgcttg ttggcaatcc
     3121 attcaataaa cttcttatcg tctaaaatgt tcaataagaa ttcatccact gcttcgcttt
     3181 cagtacgctc tgcatattca gcgtattgct taactatcac ccttacttgc tctgatattt
     3241 cccaatccac agacttagcg ttcttatttt ttggctcaag aaattgcata ggaaaatgca
     3301 cccctttttg cttagtaaat tgtaaccatt ttaacacacc aatttgcatt cctacaataa
     3361 ttatttcacg ttcgcatttt tatatacatt ttaacttaca ttttgtggtg taaattaaca
     3421 acaaacgttg atttaacaat aaaaaacgag tatcaaattc agttgcaatt tgatactcat
     3481 ttaggtttac attttcactt tttgtactcc aaaactgaac tacttaaatc tatactgctg
     3541 cctctttttt ttagtacatg tccttcatct tcgcaggaag tgccaatatg attttaagaa
     3601 aggtaataga caaaaagtca aaaagataaa acagagtttc ttctttacaa agggaatgta
     3661 tgttcgtata atggtattga ggtgaaggtt atggagaaaa aatatggaac agtagataag
     3721 atccgtactt tgcaagttag tgccagaccg ttgatccgtt tcagtttaaa tggggtcaat
     3781 tgtttgattg cttcccatag tttgaacttc ctagctgaag tagacgaagg gatgaagtta
     3841 gtcgttacag gttattataa taatagaaag caatttgttg tgagatccta tcatgtgtta
     3901 gggaaaccca aaattgtggt agaatatgag aaaagtctat acccaagaaa aaaggtccga
     3961 caagatgaaa gaataaacta gaaaacaagc ggctgagaca aaaatgtcct agccgcttgt
     4021 tttggaaaga tggatgcaga cgatgggatt tgaacccaca cgagcatacg ctcacaagaa
     4081 cctcaatcta tatataacta catgtattta agtatttgtg atgaatcact aaactttcca
     4141 aactcatttg agggatattg cttaaagatt gaacagatca attcgaagcc ctattagtat
     4201 tcgctcaatg gggggatcag aggccataaa aagagactag tcattactag tctcaggtag
     4261 tggaattgta ggattttaaa cgttcctgga gagtaccggt atgtaactcg aagaggtgat
     4321 tatcatagtc gtaaaagtaa attgaagaag cctcgccttt cacacgagaa cgtcctggtt
     4381 cgacagttaa acctaacatt tggatttttg agacaaacga atctatatct tgttcgtcaa
     4441 ttttaaaagc aatatgattg tatgtctttg gcagtttatt cgacgaattc tgcattactg
     4501 caatccatag gtccttaact aagaaaaatt tttctggata taatgaaaat ttttttgttg
     4561 actatcgtag atttcttttg catcaaataa ggtatttaac aattaagtat ttaaaagata
     4621 acttttaagt tatctttaag
//
LOCUS       DAZKOR010000269         1809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.guided.193, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000269 DAZKOR010000000
VERSION     DAZKOR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.guided.193"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..524
                     /gene="vanZ-A"
                     /locus_tag="ACG8ZM_003020"
     CDS             39..524
                     /gene="vanZ-A"
                     /locus_tag="ACG8ZM_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0493873.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            1043..1327
                     /locus_tag="ACG8ZM_003021"
     CDS             1043..1327
                     /locus_tag="ACG8ZM_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0493874.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
BASE COUNT          687 a          253 c          311 g          558 t
ORIGIN      
        1 atgatacaac ttaaacaaaa gaattagagg aaattatatt gggaaaaata ttatctagag
       61 gattgctagc tttatattta gtgacactaa tctggttagt gttattcaaa ttacaataca
      121 atattttatc agtatttaat tatcatcaaa gaagtcttaa cttgactcca tttactgcta
      181 ctgggaattt cagagagatg atagataatg ttataatctt tattccattt ggcttgcttt
      241 tgaatgtcaa ttttaaagaa atcggatttt tacctaagtt tgcttttgta ctggttttaa
      301 gtcttacttt tgaaataatt caatttatct tcgctattgg agcgacagac ataacagatg
      361 taattacaaa tactgttgga ggctttcttg gactgaaatt atatggttta agcaataagc
      421 atatgaatca aaaaaaatta gacagagtta ttatttttgt aggtatactt ttgctcgtat
      481 tattgctcgt ttaccgtacc catttaagaa taaattacgt gtaagatgtc taaatcaagc
      541 aatctgatct ttcatacaca taaagatatt gaatgaattg gattagatgg aaaacgggat
      601 gtggggaaac tcgcccgtag gtgtgaagtg aggggaaaac cggtgataaa gtaaaaagct
      661 tacctaacac tatagtaaca aagaaagccc aattatcaat tttagtgctg aggaattggt
      721 ctctttaata aatttcctta acgttgtaaa tccgcatttt cctgacggta cccccactag
      781 cagccatgct ttttttgaat ctttgtagga cgttttaaag agaattaccc actttaaaat
      841 taaacatgcg cttagaatcg ctttaggaaa cacgatccag tccaataatc gtcgataaaa
      901 acttttgaaa aaggttggtg aaattaccta cttttggaat aatcacaaat cacaagtgat
      961 taatcacttg tttattaaga tattaaaagc tataatttaa ataaagcgtg aattttatta
     1021 cacaaaaaga ggggggagaa acttggaact agcatttaga gaaagcttaa aaaagatgag
     1081 aggtaccaaa tcaaaagaaa aattctccca agaattagaa atgagtagat caaattattc
     1141 acgaatagaa tcaggaaaat cagatccaac cataaaaaca ctagaacaaa ttgcaaagtt
     1201 aactaactca acgctagtag tggatttaat cccaaatgag ccaacagaac cagaaccaga
     1261 aacagaatca gaacaagtaa cattggattt agaaatggaa gaagaaaaaa gcaatgactt
     1321 cgtgtgaata atgcacgaaa tcgttgctta ttttttttaa aagcggtata ctagatataa
     1381 cgaaacaacg aactgaatag aaacgaaaaa agagccatga cacatttata aaatgtttga
     1441 cgacatttta taaatgcata gcccgataag attgccaaac caacgcttat cagttagtca
     1501 gatgaactct tccctcgtaa gaagttattt aattaacttt gtttgaagac ggtatataac
     1561 cgtactatca ttatataggg aaatcagaga gttttcaagt atctaagcta ctgaatttaa
     1621 gaattgttaa gcaatcaatc ggaaatcgtt tgattgcttt ttttgtattc atttatagaa
     1681 ggtggagttt gtatgaatca tgatgaatgt aaaacttata taaaaaatag tttattggag
     1741 ataagaaaat tagcaaatat ctatacacta gaaacgttta agaaagagtt agaaaagaga
     1801 aatatctac
//
LOCUS       DAZKOR010000270         1325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.guided.211, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000270 DAZKOR010000000
VERSION     DAZKOR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.guided.211"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            260..1171
                     /locus_tag="ACG8ZM_003022"
     CDS             260..1171
                     /locus_tag="ACG8ZM_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0493875.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          480 a          192 c          264 g          389 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atatt
//
LOCUS       DAZKOR010000271         1229 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.guided.214, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000271 DAZKOR010000000
VERSION     DAZKOR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.guided.214"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1103
                     /locus_tag="ACG8ZM_003023"
                     /note="aph(2'')-Ia"
     CDS             <1..1103
                     /locus_tag="ACG8ZM_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFQ0493876.1"
                     /translation="TRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIE
                     DLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSV
                     AYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILG
                     YKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEE
                     YILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLT
                     GIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEY
                     YPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          529 a           95 c          201 g          404 t
ORIGIN      
        1 gtacaagata tattaaattg atttttgaat ttttgaaaaa agaaagaaat gctaatgcag
       61 ttattttaga ccctcataaa aataatccaa gagcaataag ggcataccaa aaatctggtt
      121 ttagaattat tgaagatttg ccagaacatg aattacacga gggcaaaaaa gaagattgtt
      181 atttaatgga atatagatat gatgataatg ccacaaatgt taaggcaatg aaatatttaa
      241 ttgagcatta ctttgataat ttcaaagtag atagtattga aataatcggt agtggttatg
      301 atagtgtggc atatttagtt aataatgaat acatttttaa aacaaaattt agtactaata
      361 agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt tttaaataca aatttagaaa
      421 ctaatgtaaa aattcctaat attgaatatt cgtatattag tgatgaatta tctatactag
      481 gttataaaga aattaaagga acttttttaa caccagaaat ttattctact atgtcagaag
      541 aagaacaaaa tttgttaaaa cgagatattg ccagtttttt aagacaaatg cacggtttag
      601 attatacaga tattagtgaa tgtactattg ataataaaca aaatgtatta gaagagtata
      661 tattgttgcg tgaaactatt tataatgatt taactgatat agaaaaagat tatatagaaa
      721 gttttatgga aagactaaat gcaacaacag tttttgaggg taaaaagtgt ttatgccata
      781 atgattttag ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg
      841 attttggaga ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata
      901 gtgaagaaga aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag
      961 atattgagaa agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta
     1021 ttgtttatgg aattaaaaat attaaacagg aatttatcga aaatggtaga aaagaaattt
     1081 ataaaaggac ttataaagat tgattatata atatatgaaa agctattata aaagacatta
     1141 gtattaaata gtttaaaaaa atgaaaaata ataaaggaag tgagtcaatt ccagactcct
     1201 gtgtaaaatg ctatacaatg tttttacca
//
LOCUS       DAZKOR010000272         1084 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.guided.221, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000272 DAZKOR010000000
VERSION     DAZKOR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.guided.221"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..194
                     /locus_tag="ACG8ZM_003024"
     CDS             111..194
                     /locus_tag="ACG8ZM_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0493877.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG8ZM_003025"
     CDS             319..1056
                     /gene="erm(B)"
                     /locus_tag="ACG8ZM_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0493878.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          410 a          175 c          167 g          332 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa aattttgtat aataggaatt
       61 gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gggattcgtc atgttggtat
      121 tccaaatgcg taatgtagat aaaacatcta ccgttttgaa acagactaaa aacagtgatt
      181 acgcagataa ataaatacgt tagattaatt cctaccagtg actaatctta tgacttttta
      241 aacagataac taaaattaca aacaaatcgt ttaacttctg tatttattta tagatgtaat
      301 cacttcagga gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt
      361 gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac
      421 gaaattggaa caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta
      481 acgtctattg aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaac
      541 actcgtgtca ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg
      601 tataaaattg ttgggaatat tccttaccat ttaagcacac aaattattaa aaaagtggtt
      661 tttgaaagcc atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc
      721 ttggatattc accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg
      781 cttaagctgc cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa
      841 cttacccgcc ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt
      901 tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca
      961 atgaaacacg ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt
     1021 tttaatagtt atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt
     1081 tgga
//
LOCUS       DAZKOR010000273         1010 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.guided.224, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000273 DAZKOR010000000
VERSION     DAZKOR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.guided.224"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..302
                     /locus_tag="ACG8ZM_003026"
     CDS             <1..302
                     /locus_tag="ACG8ZM_003026"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0493879.1"
                     /translation="KENNKIIGFAYCYTLLRPDGKTMFYLHSIGMLPNYQDKGYGSKL
                     LSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            303..>1010
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG8ZM_003027"
     CDS             303..>1010
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG8ZM_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HFQ0493880.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKG"
BASE COUNT          419 a           81 c          176 g          334 t
ORIGIN      
        1 ctaaagaaaa taataaaatt ataggatttg catattgcta tacactttta agacctgatg
       61 gaaaaacaat gttttattta cactcaatag gaatgttacc taactatcaa gacaaaggtt
      121 atggttcaaa attattatct tttattaagg aatattctaa agagattggt tgttctgaaa
      181 tgtttttaat aactgataaa ggtaatccta gagcttgcca tgtatatgaa aaattaggtg
      241 gtaaaaatga ttataaagat gaaatagtat atgtatatga ttatgaaaaa ggtgataaat
      301 aaatgaatat agttgaaaat gaaatatgta taagaacttt aatagatgat gattttcctt
      361 tgatgttaaa atggttaact gatgaaagag tattagaatt ttatgatggt agagataaaa
      421 aatatacatt agaatcatta aaaaaacatt atacagagcc ttgggaagat gaagttttta
      481 gagtaattat tgaatataac aatgttccta ttggatatgg acaaatatat aaaatgtatg
      541 atgagttata tactgattat cattatccaa aaactgatga gatagtctat ggtatggatc
      601 aatttatagg agagccaaat tattggagta aaggaattgg tacaagatat attaaattga
      661 tttttgaatt tttgaaaaaa gaaagaaatg ctaatgcagt tattttagac cctcataaaa
      721 ataatccaag agcaataagg gcataccaaa aatctggttt tagaattatt gaagatttgc
      781 cagaacatga attacacgag ggcaaaaaag aagattgtta tttaatggaa tatagatatg
      841 atgataatgc cacaaatgtt aaggcaatga aatatttaat tgagcattac tttgataatt
      901 tcaaagtaga tagtattgaa ataatcggta gtggttatga tagtgtggca tatttagtta
      961 ataatgaata catttttaaa acaaaattta gtactaataa gaaaaaaggt
//
LOCUS       DAZKOR010000274          834 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3421
            SAMEA115706705-rid22579813.guided.239, whole genome shotgun
            sequence.
ACCESSION   DAZKOR010000274 DAZKOR010000000
VERSION     DAZKOR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706705
            Sequence Read Archive: ERR13196440
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287739.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287739.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:04:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,028
            CDSs (total)                      :: 2,968
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 44 of 89
            Pseudo Genes (incomplete)         :: 45 of 89
            Pseudo Genes (internal stop)      :: 21 of 89
            Pseudo Genes (multiple problems)  :: 17 of 89
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706705-rid22579813.guided.239"
                     /isolate="VRE3421"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            221..769
                     /gene="aac(6')"
                     /locus_tag="ACG8ZM_003028"
     CDS             221..769
                     /gene="aac(6')"
                     /locus_tag="ACG8ZM_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0493881.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          297 a          125 c          178 g          234 t
ORIGIN      
        1 atctatatca tgatgaacaa acagagtttt ttcctccaat tgatggcttt cctgatcttt
       61 cctattttga ataaagtgtt tctgtataat tttttatttt ataagtaaga taaataatga
      121 aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt cgttttttca
      181 aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca gtgaatttga
      241 ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga cttggccgga
      301 agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag aacgaatcgc
      361 ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc ctcaatacgg
      421 tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa agaaccaaat
      481 aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag gaatcacgat
      541 ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg acctgtatga
      601 gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt atgaattcta
      661 tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc
      721 ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat aaaaagaggt
      781 tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt actt
//
